LOCUS       DAWTCI010000001       137782 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWTCI010000001 DAWTCI010000000
VERSION     DAWTCI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 137782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 137782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..137782
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.001"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(320..967)
                     /gene="adk"
                     /locus_tag="WM481_000001"
     CDS             complement(320..967)
                     /gene="adk"
                     /locus_tag="WM481_000001"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFA9079431.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVG
                     LDAVVEIDVSDSVIVDRMSGRRVHLASGRTYHVTYNPPKTEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQAVEAVKAEVLGALGK"
     gene            1182..2021
                     /gene="htpX"
                     /locus_tag="WM481_000002"
     CDS             1182..2021
                     /gene="htpX"
                     /locus_tag="WM481_000002"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFA9079432.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(2257..3555)
                     /locus_tag="WM481_000003"
     CDS             complement(2257..3555)
                     /locus_tag="WM481_000003"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFA9079433.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHEGLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQYLHNAGPVKAEDVMAVIEKVAPRIAPMIADVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPGGGKTDILPCGSDAVETCKPIYETMPGWRESTVGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(3660..4811)
                     /locus_tag="WM481_000004"
     CDS             complement(3660..4811)
                     /locus_tag="WM481_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFA9079434.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLPNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGSVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDNEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAVERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQIVKR"
     gene            5146..6525
                     /locus_tag="WM481_000005"
     CDS             5146..6525
                     /locus_tag="WM481_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     NorM"
                     /protein_id="HFA9079435.1"
                     /translation="MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTGEAGETGRQGIW
                     FGLILGIFGMILMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGVATMAVFWFSALALW
                     IYIAKEKFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWVL
                     AVITVLSLVLFRSPLASMYNDDPAVLSIASTVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAVALVWCLEKYSM
                     ELVKSHKAV"
     gene            6569..7609
                     /gene="murB"
                     /locus_tag="WM481_000006"
     CDS             6569..7609
                     /gene="murB"
                     /locus_tag="WM481_000006"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFA9079436.1"
                     /translation="MQPIRYRTDLTPYNTFGLHAQARAFIALKHADELRDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYDGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHT
                     VALGLSGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAKVAELSAGREATAKDVS
                     DAVSAIRNSKLPDPKVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLANKNNASSDDIWKLAQHVCNTVFTRFQVELH
                     AEPNWLPASFSL"
     gene            7621..8271
                     /locus_tag="WM481_000007"
     CDS             7621..8271
                     /locus_tag="WM481_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFA9079437.1"
                     /translation="MPVTRIAKTNTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVKLLTQLNGLNVINADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGVRRTLSLLRPYLLPEHRAEYDRKISNGNP"
     gene            8326..9102
                     /locus_tag="WM481_000008"
     CDS             8326..9102
                     /locus_tag="WM481_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9079438.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYRHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLNRTDTFQTALWRHHADKPVWFFLLPPSFLAHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDKARECNETAAPDPQTESARQILAAEQHLLDNGVPNIDILRLGG
                     LYCAERHPVSRLVQKQNIQGGNRPVNIVHRDIAVENLFQTASNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFSPDDSVGKIIRTVCDNGLSL"
     gene            9152..9718
                     /locus_tag="WM481_000009"
     CDS             9152..9718
                     /locus_tag="WM481_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFA9079439.1"
                     /translation="MSALLPIINRLILQSPDSRSELTSFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQAGIGSRATDIGHGIKQIGRNIAEQIGGFSREPESANTGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            9738..10628
                     /locus_tag="WM481_000010"
     CDS             9738..10628
                     /locus_tag="WM481_000010"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFA9079440.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGV
                     RECCIYTQDTDGCHIVNKTELGQYCDLVAVLGGDGTFLSAAREITPRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERALALNDAVLSRGG
                     AGQMIEFEVFVNQEFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQSFIDVQNLDRIIIRRYHNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            10653..11411
                     /locus_tag="WM481_000011"
     CDS             10653..11411
                     /locus_tag="WM481_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9079441.1"
                     /translation="MNSKISSDHTEDTIRLSKRMAQLGLCSRREADGHIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NCWEGDTSRISFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGDKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(11466..12130)
                     /locus_tag="WM481_000012"
                     /pseudo
     CDS             complement(11466..12130)
                     /locus_tag="WM481_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(12327..13124)
                     /locus_tag="WM481_000013"
     CDS             complement(12327..13124)
                     /locus_tag="WM481_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HFA9079442.1"
                     /translation="MNIKDKQRMNAIADVQSSRDLRNLPINQVGIKDLRFPITLKTAE
                     GTQSTVARLTMTVYLPAEQKGTHMSRFVALMEQHTEVLDFAQLHRLTAEMVALLDSRA
                     GKISVSFPFFRKKTAPVSGIRSLLDYDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKE
                     ISQYGAHNQRSHVTVSLTSDAEVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYE
                     NPKFVEDMVRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            13161..13307
                     /locus_tag="WM481_000014"
     CDS             13161..13307
                     /locus_tag="WM481_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079443.1"
                     /translation="MPSEKTLSAVLPANAGLYHLLLAAALIVSIFGIVIKSRLIHILQ
                     GRNR"
     gene            13304..14461
                     /locus_tag="WM481_000015"
     CDS             13304..14461
                     /locus_tag="WM481_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HFA9079444.1"
                     /translation="MKFPTRAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHNVKLVWLEMPSNPLLRLVDIKALAA
                     KAKAADALVGIDNTFATPYLQQPLDMGCDFAFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPTDCWLVLRGIKTLALRMEAHCKNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYALAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMTSSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSIGIEDVDDIWNDISAALDTTL"
     gene            complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="WM481_000016"
     CDS             complement(14525..15526)
                     /gene="hemB"
                     /locus_tag="WM481_000016"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFA9079445.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLE
                     GAAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGTQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="WM481_000017"
     CDS             complement(15710..15994)
                     /gene="yhbY"
                     /locus_tag="WM481_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFA9079446.1"
                     /translation="MADTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICNTLCEAVDAQPVRHIGKLLVLWRKNIEA"
     gene            16104..16724
                     /locus_tag="WM481_000018"
     CDS             16104..16724
                     /locus_tag="WM481_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFA9079447.1"
                     /translation="MAVRSKSSKAWLHEHINDQYVHMAQKDGYRARAAYKLLEINEKD
                     KIIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            16788..18755
                     /gene="ftsH"
                     /locus_tag="WM481_000019"
     CDS             16788..18755
                     /gene="ftsH"
                     /locus_tag="WM481_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFA9079448.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIRQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREKTEAPAPADTASTESGQQPENKA"
     gene            18977..19408
                     /locus_tag="WM481_000020"
     CDS             18977..19408
                     /locus_tag="WM481_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFA9079449.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMRVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            19401..19679
                     /locus_tag="WM481_000021"
     CDS             19401..19679
                     /locus_tag="WM481_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFA9079450.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRTAALQSGLDGIFENLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            19732..20310
                     /gene="pth"
                     /locus_tag="WM481_000022"
     CDS             19732..20310
                     /gene="pth"
                     /locus_tag="WM481_000022"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFA9079451.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEHR
                     RQIDDAVAKSLQAVPDIISGKWEEATRFLHSK"
     gene            20371..20577
                     /locus_tag="WM481_000023"
     CDS             20371..20577
                     /locus_tag="WM481_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFA9079452.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            20574..20936
                     /locus_tag="WM481_000024"
     CDS             20574..20936
                     /locus_tag="WM481_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFA9079453.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQILLLAKAFKTGKLPIPYRSLIAFAFCFAVGSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFAARSACRPD"
     gene            21165..22580
                     /locus_tag="WM481_000025"
     CDS             21165..22580
                     /locus_tag="WM481_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HFA9079454.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITITALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFGSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIAASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            22700..23209
                     /locus_tag="WM481_000026"
     CDS             22700..23209
                     /locus_tag="WM481_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9079455.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIESVSTKRHGYHDDVPTEP
                     VIIIKAEAV"
     gene            23377..24759
                     /locus_tag="WM481_000027"
     CDS             23377..24759
                     /locus_tag="WM481_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFA9079456.1"
                     /translation="MASITRDIFKAYDIRGIVGKTLTDDAAYFIGRAIAAKAAEKGIA
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGAYHDHI
                     VGHVKLKRPINIAIDAGNGVGGAFAGKLYKGLGNEVTELFCEVDGNFPNHHPDPSKPE
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPGAK
                     VIFDVKSTRLLAPWIKEHGGEAIMEKTGHSFIKSAMKKTGALVAGEMSGHVFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLDNLPQSISTPELNISLPEGSNGHQVIEELAAK
                     AQFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQAAIERIQNRFKAVI
                     ESNPHLIWPL"
     gene            complement(24815..25570)
                     /locus_tag="WM481_000028"
     CDS             complement(24815..25570)
                     /locus_tag="WM481_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9079457.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDG
                     GVIVEQGSPKELFDHLKHERTRRFLSQIQSAKI"
     gene            complement(25580..26296)
                     /locus_tag="WM481_000029"
     CDS             complement(25580..26296)
                     /locus_tag="WM481_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9079458.1"
                     /translation="MFNNFLASLPFMTETRADMLISAFWPMVKAGFTVSLPLAIASFV
                     IGMIIAVAVALVRIMPSGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIY
                     INPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(26286..27113)
                     /locus_tag="WM481_000030"
     CDS             complement(26286..27113)
                     /locus_tag="WM481_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9079459.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAISGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSEPYSWSGAVLVAHNDSNIKSIADIKGVKTA
                     QSLTSNYGEKAKAAGAQLVPVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            27398..28306
                     /locus_tag="WM481_000031"
     CDS             27398..28306
                     /locus_tag="WM481_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFA9079460.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFPEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDETIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            28383..31985
                     /gene="recB"
                     /locus_tag="WM481_000032"
     CDS             28383..31985
                     /gene="recB"
                     /locus_tag="WM481_000032"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFA9079461.1"
                     /translation="MSAPIRAFDPLTAPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDDTLSDGIAAYCAE
                     HHEGDTFLPALLEQALQKESRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSGDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRDAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHERLLKLSSDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEETTMIRLQLDLIEYLNSSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     PARAVAENWEIALIDEFQDTDPLQYEIFQKIFIARNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQLRWLHENDNEKANKDVLRRRAADYCADEIARALNEASGGRLNFKGRPLQ
                     SGDIAVLVRTHNEAVMISAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDIWQKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRAELLAK
                     AQLSESEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRRTYACEKDGITMLKRNWRRVADNAPAGTDFAFTEDAPPPAAYRGNADQAAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAEQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPGCRRPEMGFTLHTEDFGLKRLRDWLARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICIIDYKSNHLSAYTRQAMDEAVAHQHYYL
                     QALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
     gene            32024..32383
                     /locus_tag="WM481_000033"
     CDS             32024..32383
                     /locus_tag="WM481_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFA9079462.1"
                     /translation="MNIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIYEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            32508..32987
                     /locus_tag="WM481_000034"
     CDS             32508..32987
                     /locus_tag="WM481_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFA9079463.1"
                     /translation="MKHILPLIAASALCISTASAHPADKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRTAYTDTRALIVGSRISFDEVANTLANAEKLIQKTVGE"
     gene            complement(33262..34641)
                     /gene="radA"
                     /locus_tag="WM481_000035"
     CDS             complement(33262..34641)
                     /gene="radA"
                     /locus_tag="WM481_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFA9079464.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESFAAPEPK
                     NARFQSWAADTSTVQSLSAVTAAEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRSQRLELHAEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLSVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIHGVSSLQEA
                     IDICRDSRE"
     gene            34983..36107
                     /locus_tag="WM481_000036"
     CDS             34983..36107
                     /locus_tag="WM481_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9079465.1"
                     /translation="MLSKQISNLNSSSNKPKILSLFSGCGGLDLGFHQAGCETVWAND
                     FSHWACESFRKNIGDVIVEGDIEQINPNDPTIPDCDIILGGFPCQDFSMIWKQPGLEG
                     ERGNLYKSFLRFVNAKKPKVFVAENVKGLLTANKKKAIQQIITDFENCGYYVQAKLYN
                     FAEFGVPQFRERVLIVGVRLDTGFDFRHPEPTHNETGENGLKPYVTAGQAISNIPQNA
                     SNNELLKISDKTRRMLELIPEGGNFTDIPKDHPLYVKGMISHVYRRMHRNEPSKTIIA
                     AGGGGTWGYHFPEPRAFTNRERARLQSFPDDFEFVGSTTEVRRQIGNAVPPQGVVELA
                     KSILPIFSDNYEKVDLHEKLVEEKEILFHDRLSKIRGGKQ"
     gene            36104..37141
                     /locus_tag="WM481_000037"
     CDS             36104..37141
                     /locus_tag="WM481_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HFA9079466.1"
                     /translation="MNTVFSNIANAKITEKSLNAVWMDLFKSADEVLMATGYVSNDAV
                     VELHKILELNDHIQKIDLLVGMHYLEGFSHLQYDSLCKLNDFLRHEKRGAVYVSPFVK
                     FHGKMYSFKNYQKINGLIGSANLTCFWDSTERTYETMLHLNGKPAQILQADIQSTIHK
                     LGKNIQEVERPSKFIEHNSHLENCLGVQKIAPEQIRQLFAQTSEYHFSIPAKTEEKSN
                     LNVFFGEGRRDKRGFVKPRPWYEVELIVSKDITSQEGYPVLKSFTVITDDGWQFQCKT
                     SGDYSKNFRSENDLKTLGKWIKGRLESHGCLQNNEKITHETLREYGNDHFELRSTDNP
                     DVWLLSFKGKN"
     gene            37142..39031
                     /locus_tag="WM481_000038"
     CDS             37142..39031
                     /locus_tag="WM481_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9079467.1"
                     /translation="MLRTYLNQLTPPELADSVKNTVDGFMEKLSQTEPKIAQNVLLLG
                     NVQSGKTAQVLGVLSALADDGDHKVFLYLTTDSVDLQDQTVKRAKANLKNFIVLSEAD
                     DRSFMEVMKAENPILVVIKKNARVLKRWRNLFASQSSLKGYPLVIVDDEADAASLNTN
                     SDKPAKDASTINKLLNDIKNSCCQSLFIQLTATPQSLLLQHEESDWQPEFIHFFEAGE
                     KYIGGNFVFSDPPSYIVRFIDSELDDMKDESGEIAEGAKQALLSFLITCAEFALCDKA
                     NCNFALHPSYKIQDHQAFSKKIQAFLNDLVQAVNNGEDLAGSFKESYLDLQKTKPDIH
                     HFDEIYEKLTALLENKQISTLVVNSQTETDFDLEKGFNIIIGGNVIGRGLTIPKLQTV
                     YYSRTAKKPNADTFWQHSRIFGYDRDKSLLRLYIPFDVYYFFVQLNQANNLIIGQAKN
                     SGGNIQVIYPKNINPTRKNVLKFDSINQIVGGVNYFPLHPNEDNLSEINKILPSILKD
                     EIQSDLYQIDIEDLFLVLDKLGRYVPDDWNKEKFIAGVEALKAQRPSFKTYVLIKTGR
                     KLSRATGTMLSEDDRKLGEKYPNDLFLTLYQVVGNKDKGWQGKDFWLPNIKLPHNGLV
                     YQSAK"
     gene            complement(39094..40158)
                     /locus_tag="WM481_000039"
     CDS             complement(39094..40158)
                     /locus_tag="WM481_000039"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFA9079468.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHESDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLYKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRTEVARILTGYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(40233..41450)
                     /locus_tag="WM481_000040"
     CDS             complement(40233..41450)
                     /locus_tag="WM481_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFA9079469.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRSGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQMADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADSWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSIPARLVLAKVFDETAQSQKAEAQRNLVLA
                     SVAGENRPSAETR"
     gene            complement(41447..42781)
                     /locus_tag="WM481_000041"
     CDS             complement(41447..42781)
                     /locus_tag="WM481_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFA9079470.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAACALVLAALGLGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKTLDVRMTADDGLKTSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKAIEAPSLPSERKPEQPAKKQPAPEKAGRSPSAKGERA"
     gene            complement(42793..43557)
                     /locus_tag="WM481_000042"
     CDS             complement(42793..43557)
                     /locus_tag="WM481_000042"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFA9079471.1"
                     /translation="METAKPVMLIVRPSGRAKDDVEVCRRAGWQAEVLSPIEIETDEA
                     SLKRLPEMYARADAVFWVSPAAVETAVPYLNLSDGIKAHIAVGQGSRRALARYAGAGV
                     FAPEDGNDSEAVLRLPVWNSLPEGARVLSVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVQSLFAQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSYSSISVSDGMVFPGTSNQ"
     gene            complement(43575..43997)
                     /locus_tag="WM481_000043"
     CDS             complement(43575..43997)
                     /locus_tag="WM481_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079472.1"
                     /translation="MKKMFLSAALLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFSR"
     gene            complement(44279..45247)
                     /locus_tag="WM481_000044"
     CDS             complement(44279..45247)
                     /locus_tag="WM481_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9079473.1"
                     /translation="MVELQEIFDVSYGSKLDLNKMSSFNPTINFVGRSGKNNGVTASV
                     DLLKNTKPYPAGLLTVALGGSVLSTFLQNKPFYTAQNVAVLNPKTEMTEQQKLFYCAA
                     IFANAYRFSACGREANRTLRQLFVPSLDEIPSWVESVNLNPSAGVTEPKLKEPLDLPV
                     VRQSKRLDEIFTIQNGIAATKLKEFEQRQKDTVVYIRPASTQARTLRSYIARDSVDEK
                     HIFPCHTLFTSTNGEGSHTYSYVSTCEFVANSDVAVLTPIQSDMPIEVKLYYAKCITA
                     NRYLFSYGRKPKGEKLKSIMLPYFDQQEDFDYICRFIHTLLFSNNL"
     gene            complement(45247..46071)
                     /locus_tag="WM481_000045"
     CDS             complement(45247..46071)
                     /locus_tag="WM481_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9079474.1"
                     /translation="MFALAASNMILRGDGKANLHQSSCFMTDFQDLIKNPKPETGLKR
                     PNVGFLNPPYAQSKSDAELHELYFVKEMLDMLAEGGTGIAIIPVSCVIAPSKAKSEIV
                     KYHRLKAVMSMPSELFYPVGTVTCIVVFEAHKPHFQTVVIDPDTQEEISTKKACRKTW
                     FGYWRDDGFEKTKHLGRIDLYDRWQGIKARWLEHYLNNEVHTGESVTAFVTDNDEWVA
                     EAYLETDYSKITRADFEQVVREFALFQLLGAEVGPTENLDNESYEDDDNNDFGDDE"
     gene            complement(46101..46742)
                     /locus_tag="WM481_000046"
                     /pseudo
     CDS             complement(46101..46742)
                     /locus_tag="WM481_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(46911..47231)
                     /locus_tag="WM481_000047"
     CDS             complement(46911..47231)
                     /locus_tag="WM481_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFA9079475.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDAEGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLGKEAAEQGLIWFAEHVADARAHPGKHPNIDLLENVVQSGETLLLKP
                     LAAQ"
     gene            complement(47254..47880)
                     /gene="upp"
                     /locus_tag="WM481_000048"
     CDS             complement(47254..47880)
                     /gene="upp"
                     /locus_tag="WM481_000048"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFA9079476.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPISYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKEKGCRNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSRLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(47986..48297)
                     /gene="grxD"
                     /locus_tag="WM481_000049"
     CDS             complement(47986..48297)
                     /gene="grxD"
                     /locus_tag="WM481_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFA9079477.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVAVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(48398..48613)
                     /locus_tag="WM481_000050"
     CDS             complement(48398..48613)
                     /locus_tag="WM481_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFA9079478.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYCRSQH"
     gene            complement(48639..49418)
                     /locus_tag="WM481_000051"
     CDS             complement(48639..49418)
                     /locus_tag="WM481_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9079479.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAVAEAHEHIYCTIGVHPDSKEAEEFSIAEMAEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLAILKECKTNSGVIHCFSEDVGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRVV"
     gene            complement(49423..49773)
                     /locus_tag="WM481_000052"
     CDS             complement(49423..49773)
                     /locus_tag="WM481_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HFA9079480.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPASTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTISGSRPTFTM"
     gene            complement(49777..50754)
                     /locus_tag="WM481_000053"
     CDS             complement(49777..50754)
                     /locus_tag="WM481_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFA9079481.1"
                     /translation="MIYPWHQEQWRQIAEHWTSRPNAWLFVGKKGTGKTAFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGRGSHPDFYEITPLADEPENGRKLLRIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKKVLPAPSHGEALAYLRDRGVAEPEERLAFHSGAPLFQEEGELRELRAKLLEI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENR"
     gene            complement(50788..51900)
                     /locus_tag="WM481_000054"
     CDS             complement(50788..51900)
                     /locus_tag="WM481_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9079482.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL"
     gene            52086..52787
                     /locus_tag="WM481_000055"
     CDS             52086..52787
                     /locus_tag="WM481_000055"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFA9079483.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGRFSAYEGELAGK
                     RMVLALSGIGKVNAAVATAWLIRQFAPDCVINTGSAGGLGKGLKVGDVVIGTETEHHD
                     VDVTAFGYARGQVPQLPARFASDGILIETAKRAARTFEGAEVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFEEFLKTA
                     AASSAKMVAEIVKSL"
     gene            52929..54722
                     /gene="lepA"
                     /locus_tag="WM481_000056"
     CDS             52929..54722
                     /gene="lepA"
                     /locus_tag="WM481_000056"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HFA9079484.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHKG
                     RELASKMRELIPRQMFDIAVQAAIGSRIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            54868..55887
                     /gene="lepB"
                     /locus_tag="WM481_000057"
     CDS             54868..55887
                     /gene="lepB"
                     /locus_tag="WM481_000057"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFA9079485.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDIVEYRDKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIDQSGLNHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(55992..56099)
                     /locus_tag="WM481_000058"
                     /pseudo
     CDS             complement(55992..56099)
                     /locus_tag="WM481_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(56323..56472)
                     /locus_tag="WM481_000059"
     CDS             complement(56323..56472)
                     /locus_tag="WM481_000059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079486.1"
                     /translation="MDWQAVKTGKTKHADNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(56518..57425)
                     /locus_tag="WM481_000060"
                     /pseudo
     CDS             complement(56518..57425)
                     /locus_tag="WM481_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(57734..58666)
                     /gene="cysK"
                     /locus_tag="WM481_000061"
     CDS             complement(57734..58666)
                     /gene="cysK"
                     /locus_tag="WM481_000061"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFA9079487.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTKGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEAAEKAGKINKNTVIVEATSGNTGIGLAMVCAARGYKLAITMPESMSKER
                     KMLLRTFGAELILTPAAEGMAGAIAKAQSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWNDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSIAKVPNEAAFETARAMAEKEGILAGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(58862..59467)
                     /locus_tag="WM481_000062"
     CDS             complement(58862..59467)
                     /locus_tag="WM481_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079488.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GVKLMPGMWGMTRAAPLFIPHFYLTLGSIFFFIGYWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAMLMQVAVTAMLLAEIGR"
     gene            complement(59464..60315)
                     /gene="dapF"
                     /locus_tag="WM481_000063"
     CDS             complement(59464..60315)
                     /gene="dapF"
                     /locus_tag="WM481_000063"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFA9079489.1"
                     /translation="MKTLKFTKMHGLGNDFMVIDAVSQDFTPEDVPIAAWADRFRGVG
                     FDQLLVVGCSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSRIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVVLPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            60452..60766
                     /locus_tag="WM481_000064"
     CDS             60452..60766
                     /locus_tag="WM481_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079490.1"
                     /translation="MSRLFLLLFPWATLAACTRNIPASPAVSNCAHLHNRTVLYFDAH
                     GKPADSTAKGGFYRTVEIHDTNRILIQDFNSTGNALVKPYWSDCRKLSVFPDKHLTMS
                     HP"
     gene            60763..61431
                     /locus_tag="WM481_000065"
     CDS             60763..61431
                     /locus_tag="WM481_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9079491.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRISSYTAIGLMLGLIGQLGISLDQTRVLQNILYTASNLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSATTGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(61504..63627)
                     /gene="pnp"
                     /locus_tag="WM481_000066"
     CDS             complement(61504..63627)
                     /gene="pnp"
                     /locus_tag="WM481_000066"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFA9079492.1"
                     /translation="MMFNKYVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVFVA
                     VTTNKEVKEGQDFFPLAVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWN
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKISQDKI
                     RDVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEEITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPVREENAAE"
     gene            complement(63861..64724)
                     /locus_tag="WM481_000067"
     CDS             complement(63861..64724)
                     /locus_tag="WM481_000067"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFA9079493.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDAPI
                     PGKGEILTQISNFWFKKLAHIMPNHFTGDTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMREAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRDKAVRLYTEAAEYAKSRGIIICDTKFEFGLDEKGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIRKTVEK
                     YQEALTLLTQD"
     gene            64917..65780
                     /gene="rfbD"
                     /locus_tag="WM481_000068"
     CDS             64917..65780
                     /gene="rfbD"
                     /locus_tag="WM481_000068"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFA9079494.1"
                     /translation="MRILLTGSKSQLAHCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     VFDGKGKIPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYAGGKSVSWYE
                     FARHIFQTALQQDASFPVPELKAVSDEGSAAAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
     gene            complement(66152..66385)
                     /locus_tag="WM481_000069"
     CDS             complement(66152..66385)
                     /locus_tag="WM481_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFA9079495.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELIPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(66551..66751)
                     /locus_tag="WM481_000070"
     CDS             complement(66551..66751)
                     /locus_tag="WM481_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFA9079496.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(66923..67798)
                     /gene="xerD"
                     /locus_tag="WM481_000071"
     CDS             complement(66923..67798)
                     /gene="xerD"
                     /locus_tag="WM481_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFA9079497.1"
                     /translation="MENGLIDRLLETLWLDRRLSRNTLDSYRRDLEKIARRLSLCGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYVWMEREGMRADNPTRLLKPPKIDR
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITTLGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVRLHSVVKEHHSRN"
     gene            complement(67850..68290)
                     /locus_tag="WM481_000072"
     CDS             complement(67850..68290)
                     /locus_tag="WM481_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFA9079498.1"
                     /translation="MDMKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIVREWRKVKVAGHAQEVLETLSR"
     gene            68611..69549
                     /locus_tag="WM481_000073"
     CDS             68611..69549
                     /locus_tag="WM481_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFA9079499.1"
                     /translation="MSIRTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIYPADTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            69678..69971
                     /gene="hfq"
                     /locus_tag="WM481_000074"
     CDS             69678..69971
                     /gene="hfq"
                     /locus_tag="WM481_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFA9079500.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENKPQAAPASTLVQVETVQQPAE"
     gene            70056..70622
                     /locus_tag="WM481_000075"
                     /pseudo
     CDS             70056..70622
                     /locus_tag="WM481_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            70676..71050
                     /locus_tag="WM481_000076"
     CDS             70676..71050
                     /locus_tag="WM481_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFA9079501.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTV
                     YLLAMCCIACIVYLAKTKVLPF"
     gene            71060..71554
                     /gene="folK"
                     /locus_tag="WM481_000077"
     CDS             71060..71554
                     /gene="folK"
                     /locus_tag="WM481_000077"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFA9079502.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFINAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRL
                     LPDR"
     gene            complement(71682..72161)
                     /locus_tag="WM481_000078"
     CDS             complement(71682..72161)
                     /locus_tag="WM481_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFA9079503.1"
                     /translation="MPESIFKQISSDILRLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVETGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVLQI"
     gene            complement(72381..73118)
                     /gene="ubiE"
                     /locus_tag="WM481_000079"
     CDS             complement(72381..73118)
                     /gene="ubiE"
                     /locus_tag="WM481_000079"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFA9079504.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTIHTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTYKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(73161..73529)
                     /locus_tag="WM481_000080"
     CDS             complement(73161..73529)
                     /locus_tag="WM481_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFA9079505.1"
                     /translation="MNNIPVEIRLLKNRAVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            73649..75268
                     /gene="mnmC"
                     /locus_tag="WM481_000081"
     CDS             73649..75268
                     /gene="mnmC"
                     /locus_tag="WM481_000081"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFA9079506.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDSECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKSDKPWFALPQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLNTPYAEPLCGLYWQHGVW
                     LNPPAFVRTLLSHPLIELYENTTLTGISHDGEKWIASTPNGTFTATHIIYCTGAHSPC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SNTGWNEAEEASNRQALAHLNPALAESLFAANPNPQKHQGHAAIRCDSPDHLPVVGAL
                     GDIAAMRQTYAKLALDKNYRIDTTCPYLPNAYTNTAHGTRGLATAPICAAAVAAEILG
                     LPHLFSQRLRHALHPNRTVIRAIVRRQNLIP"
     gene            complement(75265..75414)
                     /locus_tag="WM481_000082"
     CDS             complement(75265..75414)
                     /locus_tag="WM481_000082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079507.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
     gene            complement(75727..75801)
                     /locus_tag="WM481_000083"
     tRNA            complement(75727..75801)
                     /locus_tag="WM481_000083"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75766..75768),aa:Glu,seq:ttc)
     gene            complement(75819..75895)
                     /locus_tag="WM481_000084"
     tRNA            complement(75819..75895)
                     /locus_tag="WM481_000084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75859..75861),aa:Arg,seq:acg)
     gene            complement(76006..77679)
                     /gene="recN"
                     /locus_tag="WM481_000085"
     CDS             complement(76006..77679)
                     /gene="recN"
                     /locus_tag="WM481_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFA9079508.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPALKAELREQGLLDEGGEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGGQLIDIHGQNDHHSLNQEAAQRELLDAFVGGRVQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGHSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAGRLSSETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(77863..78465)
                     /locus_tag="WM481_000086"
     CDS             complement(77863..78465)
                     /locus_tag="WM481_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFA9079509.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPETVPSDTLPN"
     gene            complement(78566..79507)
                     /gene="ylqF"
                     /locus_tag="WM481_000087"
     CDS             complement(78566..79507)
                     /gene="ylqF"
                     /locus_tag="WM481_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFA9079510.1"
                     /translation="MAIQWFPGHMNKARKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDDNSWLEWIAKKRGAVLSGGRVNYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(79588..80442)
                     /gene="rapZ"
                     /locus_tag="WM481_000088"
     CDS             complement(79588..80442)
                     /gene="rapZ"
                     /locus_tag="WM481_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFA9079511.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDLGYFCVDNLPLEMLPSLVSYH
                     IERADETELAVSVDVRSGIDIAQAREQIAYLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVVLESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLAQEM
                     VDGIERFVTRWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSGR"
     gene            complement(80423..81385)
                     /locus_tag="WM481_000089"
     CDS             complement(80423..81385)
                     /locus_tag="WM481_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HFA9079512.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANDLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(81389..81838)
                     /gene="ptsN"
                     /locus_tag="WM481_000090"
     CDS             complement(81389..81838)
                     /gene="ptsN"
                     /locus_tag="WM481_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFA9079513.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHASVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVASAEEARAILTEE"
     gene            complement(82023..82913)
                     /gene="ubiA"
                     /locus_tag="WM481_000091"
     CDS             complement(82023..82913)
                     /gene="ubiA"
                     /locus_tag="WM481_000091"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFA9079514.1"
                     /translation="MNLKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQFYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(82903..83310)
                     /locus_tag="WM481_000092"
     CDS             complement(82903..83310)
                     /locus_tag="WM481_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079515.1"
                     /translation="MNIIEIISRNRFLKQIYPSGIMDISLVSFSTDLSNCILTIRTST
                     KPSVEIEKWGLWLKDYDTVEIELRNSFIKGMKCQNWSHNNRNICQVEIKNQEDGLKII
                     RFYDNNSNWLLELEVYGLVFQGCKTYMKEGYES"
     gene            complement(83319..83414)
                     /locus_tag="WM481_000093"
     CDS             complement(83319..83414)
                     /locus_tag="WM481_000093"
                     /inference="COORDINATES: protein motif:HMM:NF026993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079516.1"
                     /translation="MRQPASKPHFNIRDSNLDTGHVDGTHGHYNF"
     gene            complement(83434..83910)
                     /locus_tag="WM481_000094"
     CDS             complement(83434..83910)
                     /locus_tag="WM481_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFA9079517.1"
                     /translation="MEHLFGKWLPDLPAPVSDGIDLPMSRLLKARSLTAALCALPHTF
                     SVELLKLGEAETEYGRRRVRDVLLKLDGTAVVQARSACSVGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSSEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            complement(83925..84572)
                     /gene="bioD"
                     /locus_tag="WM481_000095"
     CDS             complement(83925..84572)
                     /gene="bioD"
                     /locus_tag="WM481_000095"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HFA9079518.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADEQRLTMPEIFSHPASPHLAARLDGRGLDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDHIQQQAYPVILVTSGRLGSINHTLLSFV
                     VLKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLQCRLKADFPEAEWMELAKTGAV"
     gene            complement(84569..85870)
                     /gene="bioA"
                     /locus_tag="WM481_000096"
     CDS             complement(84569..85870)
                     /gene="bioA"
                     /locus_tag="WM481_000096"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFA9079519.1"
                     /translation="MPSEHQHTSSLLNFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVENQMKQMAHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKNRFDDEWDGADLQPVRALFEAHHVDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGILTKLADKTVQILKEHSK"
     gene            complement(86504..86989)
                     /gene="fxsA"
                     /locus_tag="WM481_000097"
     CDS             complement(86504..86989)
                     /gene="fxsA"
                     /locus_tag="WM481_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HFA9079520.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAVKSSGKVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFFHDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
     gene            complement(87165..87473)
                     /locus_tag="WM481_000098"
     CDS             complement(87165..87473)
                     /locus_tag="WM481_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079521.1"
                     /translation="MTNNVDLTIPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLKSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLEDLDKELC
                     "
     gene            complement(87457..87759)
                     /locus_tag="WM481_000099"
     CDS             complement(87457..87759)
                     /locus_tag="WM481_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFA9079522.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKGMVEHYYDKQR"
     gene            complement(87833..90196)
                     /locus_tag="WM481_000100"
     CDS             complement(87833..90196)
                     /locus_tag="WM481_000100"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFA9079523.1"
                     /translation="MQFSYSWLKTQANPDLSADKLEHLLTMAGLEVEEIDTAAPAFSG
                     VVVAEVKSVEKHPDADRLNVTQVDAGTGELVQIVCGAPNVKPGIKVPCSLPVAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGTNIREYLDLDDMLFT
                     LKITPNRADCLSVKGIAREVSALTQCAFTPVEIQTAPIGSEKKQAVRIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LIVRRAQNGETLACLNEKTVTLADNTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFEPEIIVGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGKLPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRITAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMSELPETRRPRFAVYNEMAARGYRE
                     VISYAFVDEQWELDFAANAAPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIACVFGKGSDGRFVQNERIGGLWYGAAMPEQWGEKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEVMNHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGVATEAGARLRS"
     gene            complement(90287..91036)
                     /locus_tag="WM481_000101"
     CDS             complement(90287..91036)
                     /locus_tag="WM481_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9079524.1"
                     /translation="MKKWTRDETLVALYLYYIIPFQKVSKDNPVIQEYAEILGRTPSA
                     LGMKIGNLGRLDPTLKVKNISGLSNGSKMDVVVWNEFSGDWEQLNKEFEGVISQYQSN
                     DENSNIEIESPEIPKGRERFARISVRVNQGFFRSSVLAAYNNQCCITGLKQPELLVAS
                     HIKPWGEDKDNRLNPRNGLCLNALHDKAFDRGLLGIDENFKIIFSPLLAKTEGFDDLF
                     KPYENRMIRLPERLNPSLEFLKFHRENIFQS"
     gene            complement(91042..91287)
                     /locus_tag="WM481_000102"
     CDS             complement(91042..91287)
                     /locus_tag="WM481_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079525.1"
                     /translation="MRLSDYILNNKININNLSDKQVQEIIDYFSSEIDDWLLASEKEL
                     KDYDVYLYKLVLDGQQYVQGIRKSVLYDFLMENQKDS"
     gene            complement(91301..91639)
                     /locus_tag="WM481_000103"
     CDS             complement(91301..91639)
                     /locus_tag="WM481_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9079526.1"
                     /translation="MIYKNKIKEDGQDERLKEKAAQYRLFENNDTFQTALKPKFTFID
                     LFAGIGGFRIAMQNLGGEYVFSSEWDEKAKLTYEANFGEVPFGDITLEEIKQYIPKQF
                     DVLCGFTLSK"
     gene            complement(91641..92054)
                     /locus_tag="WM481_000104"
     CDS             complement(91641..92054)
                     /locus_tag="WM481_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9079527.1"
                     /translation="MDKLTPEQRKKCMQSNKSTGTKPELVLAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQKKAVIKGNREFWIAKIERNIQRDIEVTG
                     RLKAEGWTVLRFWSNDVVKNTTCCAEKVKEIIQTR"
     gene            complement(92071..93060)
                     /gene="pheS"
                     /locus_tag="WM481_000105"
     CDS             complement(92071..93060)
                     /gene="pheS"
                     /locus_tag="WM481_000105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFA9079528.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFE"
     gene            complement(93301..93660)
                     /gene="rplT"
                     /locus_tag="WM481_000106"
     CDS             complement(93301..93660)
                     /gene="rplT"
                     /locus_tag="WM481_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFA9079529.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(93673..93870)
                     /gene="rpmI"
                     /locus_tag="WM481_000107"
     CDS             complement(93673..93870)
                     /gene="rpmI"
                     /locus_tag="WM481_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFA9079530.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(94017..94538)
                     /gene="infC"
                     /locus_tag="WM481_000108"
     CDS             complement(94017..94538)
                     /gene="infC"
                     /locus_tag="WM481_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFA9079531.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(94556..96469)
                     /gene="thrS"
                     /locus_tag="WM481_000109"
     CDS             complement(94556..96469)
                     /gene="thrS"
                     /locus_tag="WM481_000109"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFA9079532.1"
                     /translation="MLNITLPDCSVRQYESPVTVAQIAASIGAGLAKAAVAGKVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     EDMPEVEAMGMYHHQEYVDMCRGPHVPNIRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKDYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWRTI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVRLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFVGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVIGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(96522..96598)
                     /locus_tag="WM481_000110"
     tRNA            complement(96522..96598)
                     /locus_tag="WM481_000110"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96562..96564),aa:Val,seq:gac)
     gene            96777..97892
                     /gene="tgt"
                     /locus_tag="WM481_000111"
     CDS             96777..97892
                     /gene="tgt"
                     /locus_tag="WM481_000111"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFA9079533.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGETTREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEEFDFPGLAVGGLSV
                     GEPKPEMYRMLHAVGPMLPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKPDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAVEQGKFADWQAQFHENRARGVD"
     gene            97902..98984
                     /locus_tag="WM481_000112"
     CDS             97902..98984
                     /locus_tag="WM481_000112"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFA9079534.1"
                     /translation="MPSEAASDSILPLYPTCTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRIVELPKWLWYPILHGLVLTFRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSIADVLAELKSQGVGRLLA
                     IPLYPQYTASSSGAAVDKVCEQLLLQRNQMSVRTISRFYDDAGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDEYTVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALTGREQFYEAGGKNYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            99175..99642
                     /locus_tag="WM481_000113"
     CDS             99175..99642
                     /locus_tag="WM481_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c2"
                     /protein_id="HFA9079535.1"
                     /translation="MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKNQTDKMPSETGNPASDGIQTKPF"
     gene            complement(99639..101369)
                     /locus_tag="WM481_000114"
     CDS             complement(99639..101369)
                     /locus_tag="WM481_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFA9079536.1"
                     /translation="MDWMNSLFLLGGALLFLSVVSTTLSARLGMPLLLVFLAVGMLAG
                     EEGVGGIAFNNVVMANFISQLALAVILLDGGLRTQLSSFRIALKPASVLASWGVFATV
                     LPLGLFATFYLGLDWKFGVLMAAIVGSTDAGAVFSLLRNSGVRLNERVQATLEIESGA
                     NDPMAVFLVTALITMIMQPAESGAAAFVRMLALQIGFGLLTGWAGGKILAKLVRRLNL
                     AEGLYALMIVSGGLLVFAFTNTIGGSGFLAVYLAGIIVGNQRNRATEHVLRVMDGLAW
                     LAQATLFVMLGLLVSPAGVLDRAAEALAIAAFLMLVARPLAVFGGLWKFNYSLREKAY
                     ISWLGLRGAVPISLAMMPLVMGVPNSHLLFDVAFAVVVLSLLIQGTTIPVMARLLKVA
                     MPNKPEPKDTHDIWLAEKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFDARCFALIR
                     NGSRIEMQSDTVLQAEDLAWYILPDGKVDKMAKYFTETGIGVRENFDFFGEFVVSPAA
                     RSGDLALAYGLKLEAGEEGLSLAELFDKRSDSQEPVEGDRIDIGGFMLTAKEVDGDGN
                     IGSMGLKVPR"
     gene            101564..102304
                     /locus_tag="WM481_000115"
                     /pseudo
     CDS             101564..102304
                     /locus_tag="WM481_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            102373..103911
                     /gene="lnt"
                     /locus_tag="WM481_000116"
     CDS             102373..103911
                     /gene="lnt"
                     /locus_tag="WM481_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFA9079537.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFAFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAASSAYLFGLTAYTAQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPQGIKTGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLAIDNTARSGKRLLPAILIAALLAAGYTAQQT
                     DFTRPDGSRSTVALLQGNIAQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPI
                     MRQNLPENILAQFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPLLTTPLYKMMDMPLSDFRKGGGKQSAMMMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANVSNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYIGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            104175..105038
                     /gene="rpoH"
                     /locus_tag="WM481_000117"
     CDS             104175..105038
                     /gene="rpoH"
                     /locus_tag="WM481_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFA9079538.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNVLSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(105195..105671)
                     /locus_tag="WM481_000118"
     CDS             complement(105195..105671)
                     /locus_tag="WM481_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFA9079539.1"
                     /translation="MESRAFVAARKGNVSSKISKTRTGYGFFAKMIFASRWLQVPIYA
                     GLTVVRAICAYKFLKSLKYLVMNLDVSDENAIMLAVLNLIDVVMIANLLTMVQIGGYE
                     SFVSRLRIDDHPDRPEWLSHVNAPVLKVRLSMSIIGIHPSIYSKHLSIPPICRKSS"
     gene            105771..106655
                     /locus_tag="WM481_000119"
     CDS             105771..106655
                     /locus_tag="WM481_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HFA9079540.1"
                     /translation="MSSGNIRIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRHLENSIREQISSHLARGKVECKIQIQDAENGNQSLELNRVLVGQLAEINKNLR
                     KHYDLAKLGVADILRFPGVLASQKENTEALAKTIVELADKALKDFTAARRREGKKLGE
                     HLLQRIKNMEEIIDALSEIFPALVEAHKEKIRTRLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(106746..107261)
                     /locus_tag="WM481_000120"
     CDS             complement(106746..107261)
                     /locus_tag="WM481_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9079541.1"
                     /translation="MMILKTAPLKRRFAAILYEMLLVGAATCLAALLAGIAAIFLNPV
                     SIAVSALVTSILIMGAWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALVWLILPWGFALLNPDRQFLYDFLA
                     GTRLVDVKEQS"
     gene            complement(107337..107756)
                     /locus_tag="WM481_000121"
     CDS             complement(107337..107756)
                     /locus_tag="WM481_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079542.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(107877..108875)
                     /locus_tag="WM481_000122"
     CDS             complement(107877..108875)
                     /locus_tag="WM481_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFA9079543.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEAVDALRAAAKKQGY
                     LNREAYTADASFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKNGGEALQDFAARLP
                     EDTVTLVLLPKLEKTRLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHAVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVPRVCRLLDGLEEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKQLVVSLAE"
     gene            complement(108877..109356)
                     /locus_tag="WM481_000123"
     CDS             complement(108877..109356)
                     /locus_tag="WM481_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079544.1"
                     /translation="MNKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRILDYADNEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAE"
     gene            complement(109408..110187)
                     /gene="pgeF"
                     /locus_tag="WM481_000124"
     CDS             complement(109408..110187)
                     /gene="pgeF"
                     /locus_tag="WM481_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFA9079545.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPETVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWSGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTSMPEAAAAFEPIGGGKFLADLYALARLILRREG
                     VGGVYGGTHCTVLERDIFFSYRRDGATGRMASLIWLDGNAV"
     gene            110372..110563
                     /locus_tag="WM481_000125"
     CDS             110372..110563
                     /locus_tag="WM481_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079546.1"
                     /translation="MLQQGTAHQAPHCVLPERGCPPIRFYRYKQTGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
     gene            110781..111587
                     /locus_tag="WM481_000126"
     CDS             110781..111587
                     /locus_tag="WM481_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFA9079547.1"
                     /translation="MFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLPSKL
                     LNLPAEIAVGVILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLM
                     LAGEMLEIQAAGMLMSIVKMVLLPIVLGLIVHKVLGSKTEKPTDALPLVSVAAIVLII
                     GAVVGASKGKIMESGLLIFAVVVLRNGIGHLLGFFAAKWTGLPYDAQKTLAIEVGMQN
                     SGLAAALAAAHFAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(111740..112864)
                     /gene="rluD"
                     /locus_tag="WM481_000127"
     CDS             complement(111740..112864)
                     /gene="rluD"
                     /locus_tag="WM481_000127"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFA9079548.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPES
                     MDLDIVYEDDTVIVVNKPTGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAAVKFGGKPAVTHVKVLERYLAHSYIECSLGTGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            112863..113666
                     /locus_tag="WM481_000128"
     CDS             112863..113666
                     /locus_tag="WM481_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFA9079549.1"
                     /translation="MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESL
                     AILELAYKKLDKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(113736..115955)
                     /locus_tag="WM481_000129"
     CDS             complement(113736..115955)
                     /locus_tag="WM481_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFA9079550.1"
                     /translation="MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVTDMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRP
                     GLWQAFRPLGLTHLVSISGLHVTMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VSIPWFSWVLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVSYRPEAVPENEAAVTVWDAGQGLSVL
                     VRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRVYKFYWQKKPFE"
     gene            115987..116262
                     /locus_tag="WM481_000130"
     CDS             115987..116262
                     /locus_tag="WM481_000130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079551.1"
                     /translation="MIKQSHKTVITRFENDKNANEKPQYYPRQRQTFHRHSRKSGNPK
                     TKSRRNLSERTEPQKPASHLRLNNRTKKAEIKTSGFFFKLAIKHQNE"
     gene            116341..120765
                     /locus_tag="WM481_000131"
     CDS             116341..120765
                     /locus_tag="WM481_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFA9079552.1"
                     /translation="MKAKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWSASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRIGAGRQLVYEKGVYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQHDNAGTVKG
                     NGEHHWKTTGTNSHIGSTAVRLANNEGDANNGQNVTFEDNGTLVLDQNINQGAGGLFF
                     KGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQG
                     QLKVGDGTVILNQKADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRL
                     DANGNDLTFEHIRNVDEGARIVNHNTDHASTITLTGKSLITNPNSLSVHSIQNDYDED
                     DYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGNVNAPMPENGVAENNDWVFMGYTQEE
                     AKKNAMNHKNNQRISGFSGFFGEENEKGHNGALNLNFNGKSAQNRFLLTGGANLNGKI
                     SVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQ
                     SASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALN
                     SFDATRINGNVNLSQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLS
                     LADSHIHLNNASDAQSANKYHTIKINHLSGNGHFHYLTDLAKNLGDKVLVKESASGHY
                     QLHVQNKTGEPNQEGLDLFDASSVQDRSRLFVSLANHYVDLGALRYTIKTENGITRLY
                     NPYAGNRRPVKPAPSPAANTASQAQKATQTDGAQIAKPQDIVVAPPSPQANQAEEAKR
                     QQAKAEQVKRQQAEAGRKSAELSAKQRAGEEERRQTAQSQPQRRKRRAAPQDYMAVSQ
                     DRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDEDVSESV
                     DTSDKQPQDNTELHEKVEAVSLQPRAAQPRTQAAAQADAVSTNTNSALSDAMASTQSI
                     LLDTGASLTRHIAQKSRADAEKNSVWMSNTGYGRDYASAQYRRFSSKRTQTQIGIDRS
                     LSENMQIGGVLTYSDSQHTFDQAGGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSR
                     LQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGDDSVKVSS
                     MAVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGKSFAYKADNQQQYS
                     AGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(120841..122043)
                     /gene="trpB"
                     /locus_tag="WM481_000132"
     CDS             complement(120841..122043)
                     /gene="trpB"
                     /locus_tag="WM481_000132"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFA9079553.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALKELEQAYNEAKNDPE
                     FWAEFRRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     ARRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VIGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFYPYIEEENVRLVGVEAGGLGVDTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIEL"
     gene            complement(122126..122755)
                     /locus_tag="WM481_000133"
     CDS             complement(122126..122755)
                     /locus_tag="WM481_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFA9079554.1"
                     /translation="MSENLLEIETHPFDPVLPPKAAVMMMGTFPPKEDKRAMQFHYPN
                     FQNDMWRVYGLVFFNDAAHFRMPSHGEAAHSEKAFDAEKIKAFLHERGIASCPTVLKA
                     AREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGGKATEILLDIQGGGIKMPKTG
                     ETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAFFEMAGLCEKQL"
     gene            123031..123810
                     /gene="rsmA"
                     /locus_tag="WM481_000134"
     CDS             123031..123810
                     /gene="rsmA"
                     /locus_tag="WM481_000134"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFA9079555.1"
                     /translation="MKEHKARKRFGQNFLQDTRIIGDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGISGKKKIVG
                     NLPYNISTPLLFKLAEVADDVADMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKIDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFRQRRKTIRNN
                     LKELADDDDLQAVGISPQDRAEHIAPEKYVALSNYLADKAV"
     gene            123825..124394
                     /locus_tag="WM481_000135"
     CDS             123825..124394
                     /locus_tag="WM481_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HFA9079556.1"
                     /translation="MESNLQKTERLIREINRLHAQYSQDYFETGKVRKINLSHTLKNV
                     PTEHILSYRLNLHEAVNDYLAFADTRGIDFFYRVKTAESIHDKINRYLARGTQYPTNN
                     ILNDIFGARLIWPSETVAGILEKLDGWKTEYGLKNWYLRDAGGYIGIHIYFKNSSNFY
                     YPWELQVWDEKDAKANIENHMAYKRNFVR"
     gene            124505..124960
                     /locus_tag="WM481_000136"
     CDS             124505..124960
                     /locus_tag="WM481_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079557.1"
                     /translation="MKYTAALLTFLLTACMNPNDAFFQNRRYQMPEAQLNGSNAVFHY
                     GYSQNPDHDLLVDQVRVPDAYRASFARDWIAKEQGYRKNDCVLEKAESGGVTYYTCDA
                     EKGFNGTRFVIYVVNMKNDKGYVKVYRGMNKPNQEELTKLVADLNKFYP"
     gene            125002..125730
                     /locus_tag="WM481_000137"
     CDS             125002..125730
                     /locus_tag="WM481_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9079558.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLDNITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            125803..126162
                     /locus_tag="WM481_000138"
     CDS             125803..126162
                     /locus_tag="WM481_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079559.1"
                     /translation="MYFVDRTAAVLKPTARFLEWLKSTDENMPDLTIEQLRADCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            126167..126607
                     /locus_tag="WM481_000139"
     CDS             126167..126607
                     /locus_tag="WM481_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HFA9079560.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASIAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGRAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVRQCIMPDMTDKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            126638..127912
                     /gene="folC"
                     /locus_tag="WM481_000140"
     CDS             126638..127912
                     /gene="folC"
                     /locus_tag="WM481_000140"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFA9079561.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVSEVKKRMNLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKTGTLTSPHLLHYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFIREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLDDRLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRNLINLAYAQKRTAV
                     FSMLSDKDIDGVSETVKDQFDEWYIAPLDVPRGMTADALKAKLEQHHIENIQTFAAVR
                     DAYRAAASKAGEDDRIVVFGSFHTVADVMSVL"
     gene            127926..128966
                     /gene="ftsN"
                     /locus_tag="WM481_000141"
     CDS             127926..128966
                     /gene="ftsN"
                     /locus_tag="WM481_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9079562.1"
                     /translation="MSENKQNEVLTGYEQLKRRNRRRLVTASSLVAASCILLAAALSS
                     DPADSNPAPQAGETGATESQTANTAQTPALKSAAENGETAADKPQDLAGEDKPSAADS
                     EISEPENVGAPLVLINDRLEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATA
                     DSTDTVAVEKPKRTAEPKPQKAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAK
                     SDSAVKEAKKADKAEGKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAA
                     IQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKL
                     RVHGIAGQVTNE"
     gene            128959..129456
                     /locus_tag="WM481_000142"
     CDS             128959..129456
                     /locus_tag="WM481_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFA9079563.1"
                     /translation="MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPE
                     SLDDD"
     gene            129650..131194
                     /gene="purF"
                     /locus_tag="WM481_000143"
     CDS             129650..131194
                     /gene="purF"
                     /locus_tag="WM481_000143"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFA9079564.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVSEVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLALGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFNGTIIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLNKPYREGLIKNR
                     YIGRTFIMPGQSTRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            131294..131785
                     /gene="greB"
                     /locus_tag="WM481_000144"
     CDS             131294..131785
                     /gene="greB"
                     /locus_tag="WM481_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFA9079565.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGVDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            131844..132470
                     /locus_tag="WM481_000145"
     CDS             131844..132470
                     /locus_tag="WM481_000145"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFA9079566.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIK
                     AQKIAAALPPFVSVVALFINESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAATRFPNAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(132944..135775)
                     /gene="lbpA"
                     /locus_tag="WM481_000146"
                     /gene_synonym="tbpA"
     CDS             complement(132944..135775)
                     /gene="lbpA"
                     /locus_tag="WM481_000146"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFA9079567.1"
                     /translation="MNKKHGFPLTLTALAIATAFPAYAAQAGAAALDAAQSQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSRGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIKRKTTEPFFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDEKNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYADRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSAGRVTSSWKTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(135772..137757)
                     /locus_tag="WM481_000147"
     CDS             complement(135772..137757)
                     /locus_tag="WM481_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9079568.1"
                     /translation="MRLLRRNTAFHREDGTAIPDSKQAEEKLSFKEGDVLFLYGSKGN
                     KLQQLKSEIHKRDSDVEIRTSEKENKKYNYKFVDAGYVYTKNGKDEIEQNSGGKRFTH
                     RFGYDGFVYYSGERPSQSLPSAGTVKYFGNWQYMTDAKRHRTGKAVASDDLGYITFYG
                     NDIGATSYAAKDADDREKHPAEYTVDFDKKILKGELIKNQYVQKRNDPKEPKKPLTIY
                     NITADLNGNRFTGSAKVNTEVKTRHADKEYLFFHTDADQRLEGGFFGDNGEELAGRFI
                     SNDNGVFGVFAGKQKTNASGTNPAMPFGKHTKILDSLKISVDEATDENPRPFEVSTMP
                     DFGHPDKLLVEGREIPLVSKEKTIDLADGRKMTVSACCDFLTYVKLGRIKTERPAVKP
                     KAQDEEDSGINNGEESEDEEIAEESEDEVSEDDNGEDEDEIVEEEADEAEEIEEEAEE
                     EEPEEESPEEGNGVSDGIPPAPEALKGRDIDLFLKGIRTAEADIPKTGTAHYTGTWEA
                     RIGEPIQWDNKADKAAKAEFDVDFGNKSISGTLTEQNGVEPAFRIENGVIEGNGFHAT
                     ARTRDNGINLSGNGSTNPQSFKADNLLVTGGFYGPQAAELGGTIFNKDGKSLGITEDI
                     ENEVENEADVGEQLEPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT        34126 a        38647 c        33019 g        31990 t
ORIGIN      
        1 caacgtaaat tcaaaccgta ggccgggcat tgatgtccga cctacgcgga ctcaagcccc
       61 cgacaaactg tttcggataa atttgaaccg gtacatcagc cgtcattccc gcgcgggcgg
      121 gaatccattg ataaaactca gtttatcaga tttaaacagc gattgcccaa attcagaaat
      181 ggattcccgc ctgcgcggga atgacgccga atgattattt ttcggttgca acacgctgtt
      241 tatcaaaaac aggtcgtctg aaaacctgat tttctgtttt cagagtgcga agcctgcccg
      301 tgggtacgac cttttcgatt tatttgccca atgcgcccaa tacttcggct ttcacagctt
      361 ctactgcttg ggtaccgtcg actttgatgt atttcggcgc gtgttcgcct tccagcttgc
      421 tgtaaaaatc aaccaaaact tcggtttgct cgtggtaaac ggcaagacgt tttttcacgg
      481 tttcttcttt gtcgtcgtcg cgctgaatca aatcttcgcc ggttacgtcg tctttgcctt
      541 cggttttagg cggattgtag gtaacgtggt aagtacggcc ggaagccaaa tgcacgcggc
      601 ggccgctcat gcggtcgaca atcacgctgt cggatacgtc gatttcaacg accgcgtcca
      661 aacccacgcc tgcttccacc atcgcttcgg cttgcgccaa tgtacgcggg aaaccgtcga
      721 acaggaaacc gtttttgcag tcgtcttgcg cgatgcgttc tttgaccatg ccgataatga
      781 tgtcgtcgcg caccaaaccg ccttcatcga tgattttttt cgcttccaaa cccaagggcg
      841 tgcctgcctt aatcgcggca cggagcatgt cgccggtaga gatttgcgga atgccgaacg
      901 cggcggtgat gaattgcgcc tgagtgcctt tgcccgcgcc cggtgcgcct aaaagtaatg
      961 ctttcatgaa gtgatccttt taaatgtgtt tattggaatc aaacagatgg tttacgattc
     1021 tacacggctt ggcgcaatag ttcaaaaacc aactgcacaa actgccattt gcaagcaaat
     1081 gttttgcata cctttgccga gcctgatatt cacaaggcgg caactttttg ggacaatccc
     1141 caaagtttca aaacatcaca ccctaccaaa aaggaacatc cgtgaaacgc atttttctgt
     1201 ttttggctac caatatcgct gttttggtcg taatcaacat tgttttggcg gttctgggca
     1261 tcaacagccg gggcggcgcg ggcagcctgt tggcgtattc cgccgtcgtc ggcttcactg
     1321 gttcgattat ttcgctgctg atgtccaaat ttatcgccaa acaatcggtc ggtgcggaag
     1381 tcatcgacac gccgcgcacc gaagaagaag cctggcttct gaacactgtc gaagcccaag
     1441 cgcggcaatg gaacctgaaa acgccagaag tcgccatcta ccactccccc gaacccaatg
     1501 cctttgccac gggcgcatcg agaaacagct ccctgatcgc cgtcagcacc ggtttgctcg
     1561 accatatgac gcgcgacgaa gtggaagccg tgttggcgca cgaaatggcg cacgtcggca
     1621 acggcgacat ggttacgctg acgctgattc aaggcgtggt caataccttt gtcgtgttcc
     1681 tgtcgcgcat tattgccaac ctgattgccc gaaacaacga cggcagccag tcccagggaa
     1741 cttatttcct agtcagcatg gtattccaaa tcctgttcgg cttccttgcc agcctgattg
     1801 tcatgtggtt cagccgccaa cgcgaatacc gcgccgacgc gggcgcggca aaactggtcg
     1861 gcgcaccgaa aatgatttcc gccctgcaaa ggcttaaagg caacccggtc gatttgcccg
     1921 aagaaatgaa cgcaatgggc atcgccggag atacgcgcga ctccctgctc agcacccacc
     1981 cttcgctgga caaccgaatc gcccgcctca aatcgcttta aaccgatttg aaacgacaaa
     2041 aaaccgtacg cacaagcagt acggtttttt cgtatatgcc gactgaaaac atcaggtttc
     2101 cataccggca ccaaccgtat ccatttccgc accggatacg ccgcattgat atatccccga
     2161 caaaccgtgt ccggacagaa aatatcagat aaaacagcgg taaaaatgcc gtctgaaacc
     2221 aattagggct tcagacggca tttttactgc aaaaccttat gcgaacggat gatgcagcac
     2281 aatcgtttct tcgcggtcgg ggccggtgga gacgatggcg accggcgcgc cgcagacttc
     2341 ttcaatccgt ttcaaatatg ctttggcatt ggcaggcaat gcgtcgtagc ttttcacgcc
     2401 gacagtcgat tcgcgccagc cgggcatggt ttcgtaaatc ggtttgcagg tttccaccgc
     2461 atccgaaccg caaggcagga tgtcggtttt gccgccgcct ggcaattcgt agccgacgca
     2521 gatattgatg gtttcaacgc cgtccattac atcgagtttg gtaatgcaca taccggaaat
     2581 gccgttgatt tggatggagc gtttcagggc ggcggcatca aaccagccgc agcggcgcgc
     2641 gcggccggtt accgaaccga attcgtgtcc gcgctccgcc aaacctgcgc ctacttcgtc
     2701 gaacaattcg gtcgggaacg gacccgaacc gacgcgcgtg gtataggctt tgacgatgcc
     2761 caaaacataa tccagcattt gaggacctac gcccgcgcct gccgaagccg cgccggcgag
     2821 acagttggac gaggtaacga aggggtaagt gccgtagtcg atgtccaaca acgcaccttg
     2881 cgcgccttca aacagcagtt tttcgccgtt tttgtttttt tcgttcaaca cgcgggacac
     2941 gtcggcaatc atcggcgcaa tgcgcggcgc gactttttcg ataaccgcca tcacgtcttc
     3001 cgctttaacc ggtccggcgt tgtgcaggta ttggagttgg acgttgtaat aggcaaggac
     3061 ggcatccagt ttttcacgca gtttttcagg atgcagcaaa tcggcggcgc gaatggcgcg
     3121 gcgtgccact ttgtcttcgt aggcagggcc gatgccgcgg ccggtcgtgc cgattttgcc
     3181 tttgccgcgc gatgcttcgc gggcttggtc gagcgcgatg tggtaaggca ggatcagcgg
     3241 gcaggtcggc gcgattttca gacggccttc gacgtttttc acgcctgccg cgttcaactc
     3301 gtcaatttcg cccaacaggg cttcggggga aacgacaacg cccgaaccga tgaagcaatc
     3361 caatccttcg tgcaggatac cgctcggaat caggcgcaaa atggtttttt tgccgccgac
     3421 gaccaaggta tggcccgcat tgtggccgcc ttggaagcgc acgacgccgc cggcttcttc
     3481 cgccagccag tcaacgattt tacctttacc ctcgtcgccc cactgtgcgc cgattactac
     3541 aacattttta gccatagcca tataacctat cgatattaaa aattattgcg gaaaatcccc
     3601 aaaccggtgc ggctttgcgc cccgccccgg tttgcctttc atccgctatg aacgggtatt
     3661 tagcgtttca cgatctgcca aacgccgtcc gtctttttca gacggcctgc aagctcttcc
     3721 gaaacattgt gtccgatacc gtagtcaatc acgacacact gcccttgttc acgcaaggct
     3781 tcgaccgctt cgcgcgccgc ttctgcatct tccgcatcga ccaacacggc gggctgccgt
     3841 tcgacggcgg gcaaacgccc gataaagctg cgtaagtcga aactgaatcc cgttgccggg
     3901 cgcgccctgc cgaaatatcc gcccaatccg tcataacgcc cgccgcgcgc gaccgcgtcg
     3961 tggaaatcgg cggcataggc ggcatacagc aagcccgtgt ggtaattgtc gacgcgcagc
     4021 tcggacaagt cgatatggat ttcattatcg gggaatgcgt cgcacaccgc ctgcaattcg
     4081 tccaacgcgc cgccgaccgc cgacaaatcc ggcaaccgtc cgcgcgcgtc ggacaacact
     4141 tcacgcccgc cgtacaggcg cggcagcaac gagaatgctt ttgcccacat accgtccagc
     4201 ttccaagcct tgacctgcgc ttcgaccgac ccggtatctt tatcctgcat caaggcaagc
     4261 agcgttgcgg actgccccgc atccaaatgc gccgcatcgg acaaggcgcg gaatatgccg
     4321 atatgcccca gcgaaagcag cactttgccc atatcggcaa ttttcatgct tttcagcata
     4381 aggtctatca gctcgatgtc gccacggatg tcggcaaaac cgtacatttc cgcccctgcc
     4441 tgcaagggtt cgcgcatatt cggcagaccg tcgggctgcg cgtgcaacac cggaccggca
     4501 taacacaacc ggttaatccc ttggttggcg gacaaaagat gggcatcgat acgcgccacc
     4561 tgcggcgtga tgtcggcgcg tatgcccaac tgcctgccgc tgagcctgtc cgttaccaaa
     4621 atggttttca gtgaaagccc cgcatcgata tgcgtcagca gggaatgtgc gtactccatc
     4681 agcggaggct gtaccagttc ataaccgtgt acgcggaaca gtgccaacaa ctgctcgcgc
     4741 gcgctttcaa gctgccgcgc gttcgtgggc agtacgtcgg cgatatgttc cggaagctgc
     4801 catgtctgca tgagaatctg ccttctgttt tatcctgtaa acacaaaaga ctgcccgatc
     4861 cgcaatcaga cggcatcttt gccgcaacgc atcacgccgc tgtcaaaacg gcgatgcttc
     4921 cttccgattc agaatccgaa cagcccctgt ccgacggctc aatgccgtca agtcgttaaa
     4981 accaaacttt accataaaat acacacaatc tgaatatact tttatacccc gtatccgccg
     5041 tctgacggca cggtattttt tactatataa tgtgcaccat tatttctcat cacttcccgc
     5101 tgccgttttt ttgtttcaga cggcattttt attgactgaa cacttatgct gctcgacctc
     5161 gaccgctttt ccttttccgt cttcctgaaa gaaatccgcc tgctgaccgc ccttgccctg
     5221 cccatgctgt tggcgcaggt cgcgcaggtg ggcatcggtt tcgtcgatac cgtgatggcg
     5281 ggcggtgcgg gcaaggaaga tttggcggcg gtggctttgg gcagcagcgc gtttgccacg
     5341 gtttatatta cctttatggg cattatggcg gcgctgaacc cgatgattgc ccagctttac
     5401 ggcgcgggta aaaccggtga agcaggcgaa acggggcggc aggggatttg gttcgggctg
     5461 attttgggga ttttcggcat gattttgatg tgggcggcga ttacgccgtt ccgcaactgg
     5521 ctgactttga gcgattatgt ggaaggcaca atggcgcagt atatgctgtt caccagcttg
     5581 gcgatgccgg cggcaatggt acaccgcgca ctgcacgcct acgcttccag cctgaaccgc
     5641 ccgcgcctga ttatgttggt cagctttgcg gcgtttgtgt tgaacgtgcc gctgaactat
     5701 attttcgttt acggcaaatt cggtatgccc gctttgggtg gcgcaggttg cggcgtggcg
     5761 acaatggcgg tgttttggtt cagcgcgctg gcattgtgga tttatatcgc caaggaaaaa
     5821 ttcttccgcc cgttcggact gacagcgaaa ttcggcaaac cggattgggc ggtgttcaaa
     5881 cagatttgga aaatcggcgc gcccatcggg ctgtcttatt ttttggaagc cagcgcgttt
     5941 tcgtttatcg tgtttttgat tgcgcctttc ggcgaggatt atgtggcggc gcagcaggtc
     6001 ggcatcagtt tgtcggggat tctctatatg attccgcaaa gcgtcggctc ggcagggacg
     6061 gtgcgcatcg gcttttcgct tgggcggcgc gaattttcgc gggcgcgtta tatttcagga
     6121 gtgtcgctgg tgtcgggctg ggtgctcgcc gtgattaccg tgctttcctt ggtattattc
     6181 cgttcgccgc tggcaagcat gtacaacgat gatccggcag ttttaagcat cgcctccacc
     6241 gtcctgctgt tcgccggcct gttccaaccg gcagacttca cccaatgtat cgcgtcctat
     6301 gccctgcgcg gctacaaagt caccaaggtg ccgatgttca tccacgccgc cgccttctgg
     6361 ggctgcggcc tgctgccggg ctatctgctc gcctaccgtt tcgatatggg catttacggc
     6421 ttctggacgg cattgattgc ctcgctcacc atcgcagccg tcgccttggt gtggtgcttg
     6481 gaaaaataca gtatggagtt ggtcaaatca cacaaggccg tctgaaaacg cagcacactt
     6541 caattcaaat acagttaaag ctcaaaccat gcaacccatc cgataccgaa ccgacctcac
     6601 cccctacaac actttcggcc tgcacgccca agcccgagcc tttatcgcgc ttaaacacgc
     6661 cgacgaattg cgcgacatcg tccgactgcc cgagttcgac cgcgatactg ttttatggct
     6721 gggcggcggc agcaacatcc ttttgatgca ggattacgac ggcttggtcg tccatatgga
     6781 aaacaagggc atacgcgaga ttgcgcgctc agacggcatg gttctgattg aagcgcaggc
     6841 gggcgaaatt tggcacgatt tcgtgttgca caccgtcgcg ctgggtttga gcggtttgga
     6901 aaacctgagc ctgattccgg gtacggtcgg cgcgtcgccc gtgcagaaca tcggcgcata
     6961 cggcgtggag gcgaaagatg tgattcacag cgtgcgctgc ttcgatttgg atacggaaac
     7021 ctttgtaacc ctttccaatg ccgactgccg cttcgcctac cgcgaaagcc tgttcaagca
     7081 ggaaggcaaa gggcgttatg tgattgtttc ggtcgtattt gcattaaaaa cgcattttgt
     7141 gccgaacttg ggctacggcg atttggcggc gaaggttgcc gaattgagtg caggtaggga
     7201 ggcgacggcg aaagacgttt ccgatgcggt atccgccatc cgcaacagca aattacccga
     7261 cccgaaagtt ttgggcaatg tcggcagttt ctttaaaaac cccgtcgtca gcgcggaaaa
     7321 agccgccacc ctgttgcagc ggcatcccga tatgccgcgc tatccgcagc ccgacggttc
     7381 ggtcaaactc gccgccggct ggctgatcga ccaatgccgt ctgaagggct tccaaatcgg
     7441 cggtgcggcg gtacatgacc ggcaggctct ggttttggcg aacaaaaaca atgcctcttc
     7501 agacgacatc tggaaactgg cgcaacacgt ctgcaataca gtatttactc gatttcaggt
     7561 agaattacac gccgaaccca attggctgcc cgcttcgttc agcctgtaaa tccaaggagt
     7621 atgcccgtga cccgcattgc aaaaaccaat acttacaccc gcatcatcga cgccagcctt
     7681 gcgcttttca acgaggaagg cgagcgcaac atcagcacca accatattgc cgcccacttg
     7741 ggcatcagtc cgggcaatct ctattaccac ttccgcaaca aagacgaaat catcgtccaa
     7801 ctgttcaaac gttacagcga agccctgctg gaatacctca acgaagccgt gttgccctct
     7861 gatgtggaag actctatcaa ctatatggca ggcatttacg atgtcatgtg ggaataccgc
     7921 ttcctcttta gcgacgtaaa caccctgctt gcgcgcagtg ccgaattgtt gggcgaacac
     7981 aataccttta cccaagccaa agtctcgccg cttctagtca aactgctgac ccagctcaac
     8041 gggctgaacg tcatcaacgc cgaccaaacc gctatgaacg acctcgccgt caatatgtgg
     8101 atggttacga aatactggtt cgacttcgac agttccctgc gcggccgtac caagctgacc
     8161 gaagactcca aagcacgcgg cgtccgtcgt accttaagcc tccttcgccc ctatcttttg
     8221 cccgaacacc gcgcggaata cgaccgcaaa atcagcaacg gcaacccgta aacccataac
     8281 gtccgaatca gccttcagac ggcacttacc cgacggtatc agcgaatgcg cgcagtcccc
     8341 ccgccccata tctccatcac aggcctaggt tacctcggcc tgccgctggc acaaaagttt
     8401 taccgacacg gcagccgcgt cgccgccata aaacgcagcc tgacttcgga cgacatcaac
     8461 ctgcccatac acctcgacac cttcgacctc aaccgtaccg atacctttca gacggcattg
     8521 tggcggcatc acgccgacaa acccgtctgg ttcttcctgc tgccgccctc ctttttggcg
     8581 cattacgccg acaccgtcaa acaatgggca gagcttgccc gggcgtgcaa cgtgcaacac
     8641 ctgattttca caagcagcac aagcgtttac ggcgataaag cgcgagaatg caacgaaacc
     8701 gccgcacccg atccgcaaac cgagtccgcc cgacaaatcc ttgccgccga acaacacctg
     8761 ctcgacaacg gcgttccgaa catcgacatc ctgcggctgg gcgggcttta ttgcgccgaa
     8821 cgccatcccg tcagccgcct cgtgcaaaag caaaacatcc aaggcggcaa ccggcctgtc
     8881 aatatcgtcc accgcgacat cgccgtcgaa aacctgtttc agacggcatc caaccccggc
     8941 ggcaggcggc tgaaaaacat tatcgaaccg cgccacccga cacgccgcga attttatacg
     9001 gaagaagccg ccaaactcgg cctgcccgcg ccggatttct cacccgacga cagcgtgggc
     9061 aaaatcatcc gtaccgtttg cgataacggc ttaagcctgt aaaataagcg gcaacagcaa
     9121 caacaggcat tctccgcccc aatcagaaaa tatgtccgcc ctcctcccca tcatcaaccg
     9181 cctgattctg caaagcccgg acagccgctc ggaacttacc tcctttgcag gcaaaacact
     9241 gaccctgaac attgccgggc tgaaactggc gggacgcatc acagaagacg gtttgctctc
     9301 ggcgggaaac ggctttgcag acaccgaaat taccttccgc aacagcgcga tacggaaaat
     9361 cctccaaggc ggcgaacccg gggctggcga catcaggctc gaaggcgacc tcatcctcgg
     9421 catcgcggta ctgtccctgc tcggcagcct gcgttcccgc gcatcggacg aattggcacg
     9481 gattttcggc acgcaggcag gcatcggcag ccgtgccacc gacatcggac acggcatcaa
     9541 acaaatcggc aggaacatcg ccgaacaaat cggcggattt tcccgcgaac ccgagtccgc
     9601 aaacaccggc aacgaagccc ttgccgactg cctcgacgaa ataagcagac tgcgcgacgg
     9661 cgtggaacgc ctcaacgaac gcctcgacag gctcgaacgc gacatttgga tagactaacc
     9721 ttcagacggc atccgacatg aacagccctt ttcacaacat cggtatcgta acccgcccca
     9781 acacgcccga catccaagat accgcacaca cgctgattac ctttttgaag cagcacggct
     9841 ttaccgtcta tctcgacgaa gtcggcgtaa gggaatgctg catctatacc caagacaccg
     9901 acggctgcca tatcgtcaac aagaccgaac tggggcaata ctgcgacctg gtcgccgtct
     9961 taggcggaga cggcaccttt ctctctgccg cccgcgaaat caccccccgc gccgttccga
    10021 ttatcggcat caaccaaggt catttgggct ttcttaccca aattccccgc gaatatatga
    10081 cggacaagct attgcccgtt ttagaaggaa aataccttgc cgaagagcgc atcctgattg
    10141 aagccgcact gatccgcgaa ggcaaaaccg ccgaacgcgc cctcgccctc aacgatgccg
    10201 tcctctcccg cggcggtgcc ggacagatga ttgaatttga agtcttcgtc aatcaggaat
    10261 tcgtctatac acaacgttcg gacgggctga ttgtctccac ccccaccgga tcgaccgcct
    10321 attcgcttgc cgccggcggc ccaatcatgc aggcaggatt acacgccttc acgctcgtcc
    10381 ccatctgccc gcaatctatg actaaccgcc ccatcgccat tccagacacg tccgaaatcg
    10441 aaatcctcgt tacccaaggc ggcgacgcgc gcgttcattt cgacggacaa tccttcatcg
    10501 acgtgcaaaa cctcgaccgc atcatcatcc gccgctacca caatccctta cgcatcctac
    10561 acccgaccga ctaccaatat ttcaaaaccc tgcgccaaaa actgcactgg ggcgagcaat
    10621 tagtctaagc cggccttcac ccgaaagtac ccatgaattc caaaatttcc tccgaccata
    10681 cagaagatac catacgcctc tccaaacgta tggcacaact ggggctttgt tcgcgccgcg
    10741 aagccgacgg ccatatcgaa cagggctggg taacggtcaa cggcaaaacc gccgtactcg
    10801 gtcagaaagt ttcaccggca gaccgtatcg aactgaacaa gaaagcccac gaacagcagg
    10861 cggcacgcat taccatcctg ctgaacaaac ccgtcggcta tgtcagcgca caagcggaaa
    10921 aaggctataa atccgccgcc gaactgatta cccctgaaaa ttgctgggaa ggcgacacca
    10981 gccgcatcag tttcgatccg aaacacaaaa tcggtcttgc ccccgccggc aggctcgata
    11041 tcgattcagt cggattgctg gtgttgacgc aagacggccg tatcgccaag cagcttatcg
    11101 gcgaaaacag cggcagtgaa aaagaatatt tggtgcgcgt gcgcggcaaa ttggacgaaa
    11161 aaggacttgc cttactgaat cacggattga gtttggacgg tgataaactg cgtcccgcca
    11221 aagtagaatg gcaaaatgaa gaccaactgc gcttcgtgtt gaaacagggt aaaaagcggc
    11281 aaatccgccg tatgtgcgaa ctggtcggac tgcgcgtcgt cgggctgaaa cgcatccgca
    11341 tgggcaaggt caaactcggc aggctgccgc ccggcaaatg gcgttatctc gctcccggcg
    11401 aatcgtttta aataagcatt aaaaaatgcc gtctgaaaat ccttgcgttt tcagacggca
    11461 ctgccttatg cccaaataac ggtttcttcc aagggcctgc gcgctttcgt gacgatttcc
    11521 tgaacccggt agccgaatat cgcggcaacg gacacgcccc attctgccgc atcgaacaaa
    11581 ccgaactgcc cggacaatac gcgttccata tcggcatagt tgaaaccttc caccgggcag
    11641 gaatcgatac ccgccatcgc cgcacccgtc atcatgttgg ctaacgcgat ataggtctgg
    11701 cggcatcacc agtcaaacaa ggcgcgcgaa tcgtccaaaa tcttgatgtc gtcagcttga
    11761 aacgcctgat acctcgccaa agatttttct acggcatccg gttcggtaac gccgcgccgt
    11821 ttgagacttt ccaacataaa cgggctgtcg aagcgggcat ttttcttcgc caaaaacacc
    11881 accaaatgac tggcggtatc caaagcatcc gccatacccc aagaaaacag cttgattgcc
    11941 tgtcggattt cggggttttg aaccacaaca aaccgccaag gctccgaacc gaccgaactg
    12001 ggcgacaaac gcccgagttc taaaataaac tgaaaatcct cggcactgat tttacgtgcg
    12061 catcgtaatg ccggcacgat ttgcggtttt taaatgcgga tagaacctgc tctttgctta
    12121 atactgtcat cgtgcatcct tttattactt tgattgcaaa ttgatttcaa accgatacgg
    12181 cactgccgca ccctataagc cgtccccgcc atcaagcttc acccggattt tacccatctc
    12241 tctacggctg ggaaaacaaa gcggcggcag attcgggaat aactccccaa tgcctttcag
    12301 acggcatttg gacatcgcaa acgcgcctac gggtaggcga tataggcata agccgaatgg
    12361 ttgtggatag actcgaaatt ctcgctctcg acgacgaaac tcttgatgcg tttgtcggca
    12421 atcagcgaag tagcgacatc gcgcaccata tcttccacga atttcgggtt ttcgtaggcc
    12481 ttttcggtaa cgtatttttc atcggggcgt ttgagcaggc cgtagagttg gcagctcgcc
    12541 tgcgtttcca cataatcgat aacttcctcg ataccgactt cggcatcgga agtcaggctg
    12601 acggtaacgt gcgaacgctg attatgcgcg ccatattggg aaatttcttt ggaacacggg
    12661 caaagcgagg ttacgggaat catgaccttc atactgtggc cgtatgcccc gtctttcatt
    12721 tcacccgtga ggctgacatc ataatccagt aaggaccgga taccggatac cggcgcggtt
    12781 ttcttgcgga aaaacggaaa agaaacgctg attttgccgg cgcgggaatc caaaagcgcg
    12841 accatttcgg cagtcagcct atgcaattgt gcgaaatcca agacttcggt atgttgctcc
    12901 atcaatgcga caaagcgcga catatgcgtc cctttctgct cggcgggcag ataaaccgtc
    12961 atcgtcaggc gagcaaccgt ggattgtgtt ccttcggcgg ttttcagggt aatcgggaag
    13021 cgcaggtctt taatgccgac ctgattgatc ggcaggttgc gcaaatctct gctggattgc
    13081 acgtctgcaa tggcgttcat gcgttgtttg tccttaatat tcagataatt attgagatgt
    13141 gtgtattgta tggcaggcag atgccgtctg aaaaaacgct gtcggccgtc ctgcctgcaa
    13201 atgcgggatt atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag
    13261 tgataaaatc ccgacttatc catatcttac aaggcagaaa cagatgaaat tcccgacccg
    13321 tgccatccat tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta
    13381 tcaaaacagt atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gttattcgcg
    13441 cctgagcaac ccgacccgtc agattttgga agacaccgtt gccgatttgg aacacggtgc
    13501 ggcaggtttt gcgttttcca gcggtatggc ggggattgat gccgtgtggc gtaccttcct
    13561 gcgcccgggc gataccatcg tcgcggtcgc cgatatttac ggcggcgcat atgacttatt
    13621 ggtcgatgtt tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatcccga
    13681 taatttggac gaattgctca aagcgcacaa tgtcaaactg gtttggctgg aaatgccgtc
    13741 caatccgctt ttacgcttgg tagacatcaa agcccttgca gcgaaagcca aagcagccga
    13801 cgcgctggtc ggcatcgaca acacctttgc cacgccgtat ctgcaacagc ccttggatat
    13861 gggttgcgat tttgcattcc attccgctac caaatatttg tgcggccatt ccgacgtatt
    13921 gatgggcatc gtcgttgcca aaaccaaaga gctggctcag cctttgcacg atatgatggt
    13981 gcataccggc gcgattgcag gtccgacgga ctgctggctg gtgttgcgcg gcatcaaaac
    14041 gcttgccctg cgtatggagg cacactgcaa aaacgcactc gaaattgcgc gccgtttgga
    14101 agcccatcct gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattacgc
    14161 actcgcccaa gcgcaaatgc ccaaaggcat cggcggcgtg gttacggttt atctcaaaaa
    14221 cgacacgcgc gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatgacctc
    14281 cagcctcggc ggcgtggaaa gtctggtcaa ccattgctat tcccaatctc acagcggcgt
    14341 gccgcataat gtgaaaatgg aaatgggcat cagggtcgga ctcctgcgct tctccatcgg
    14401 tattgaagac gtagatgata tttggaacga tatttccgcc gcactcgata caactttgta
    14461 aaccgtaaaa aatgccgtct gaaaccatag tttcagacgg catttcaata ttgcgaatca
    14521 aatatcaacg cttcagcatc tttgccgcct caatggcgta ataggtcaaa atcccgtccg
    14581 cacccgcacg tttgaatgcc agcaggcttt ccaaaaccac tttgccgccg tccagccagc
    14641 cgttggcaac cgccgcctgc aacatcgcat attcgcccga aacctgatag gcataagtcg
    14701 gtacgccgaa ctcgtccttc acgcggcgga caacgtccaa atacggcaaa ccgggcttca
    14761 ccatcaccat atccgcacct tcctgaatat cgagcgccac ttcatgcagc gcctcatcgg
    14821 tatttgcagg atccatctga taggtctttt tatctgcctt tcccaaattg cccgaactgc
    14881 ctaccgcatc acggaaaggg ccgtagaatg cagaagcata tttggcggaa tatgccataa
    14941 tccgcgtatg gatatgtccg gcatcctcca aagcctcgcg gatggcgccg atacgcccgt
    15001 ccatcatatc ggaaggagca acgacctgcg tgcccgcctc tgcatgacat aaagcctgtt
    15061 tcaccaagac ttctacggtt tcatcattca tcacgtaacc gttttcgtcc gtcagtccgt
    15121 cctgaccgtg caccgtataa ggatcgagcg cgacatccgt cataatgccc agttcgggaa
    15181 acctctcgcg caaggctcgg acagttgacg gcacgagtcc ttcgggattg tacgcctcct
    15241 gcgcacgccc ggttttgttt gccgtaacca cgggaaagag tgccaacatc ggaataccga
    15301 gcttcaccgc ctcttccgcc gtaaacagca gcctgtccaa actctgacgc ttcacgcccg
    15361 gcatagaagg cacatcctcc tcgcgcgccg ccccctccaa tacgaacacc ggataaatca
    15421 aatcatcggc ggtcagcata tgctcgcgca tcaggcggcg tgaaaaatca tccctgcgca
    15481 tacggcgcat acgcgaagcc ggaacattgc ggtaaggaaa ttgcataagc cctccaatca
    15541 tcctgtcaac aattcaaatc atacggaagc cgccgcaact tgcagacggc atcgagcggt
    15601 aatcaaaatc cgttttcacc ggaaagggtt tcggcacgcc gaaatgccgc gccgaaggac
    15661 agcgtgccgc ccattgaaag aacaatagca aaacaaccac ttcaggctgt caggcctcga
    15721 tattcttacg ccacaatacc aaaagtttcc cgatatgccg aacaggttgc gcgtcaaccg
    15781 cctcacacaa ggtattgcag atttcgatac gctcggcacg gtcgtcgccg aatacgcgca
    15841 ctttaatcag ctcatgcgcc gtcagtgccg catcggtttc cttgatgact gcgtccgtca
    15901 gaccctgctg accgaccatc acaacaggat ggagatggtg cgcgcgcgct ttcaattcca
    15961 aaatttcttt ggtgttcaat ttggtatccg ccattttcct actgtttgaa taaaaaggat
    16021 aatgcagcat tgtacgcgat ttggcacggc aacggcaaaa ttaacgtaca atacaccgtt
    16081 tacattccga cccgcgaacc actatggctg tacgttccaa atcctcaaaa gcgtggctgc
    16141 acgaacacat caacgaccag tacgtccata tggcgcaaaa agacggctac cgcgcccgtg
    16201 ccgcatacaa acttttggaa atcaacgaaa aagacaagat aatcaaaccc ggcacggtac
    16261 ttgccgactt gggcagcgcg ccgggaagct ggtcgcaggt tgccgccaag ctgacgggta
    16321 cttccggagc ggttttcgcc ttggacatcc tgcctatgga agccataggg ggcgtgtcct
    16381 tcattcaggg cgacttccgc gagaacgacg ttttggcgca attcgaaacc ttgttggaca
    16441 accgcccgct cgaccttgta atctgcgata tggcgcccaa tatgtcggga aacgccgtaa
    16501 gcgatcaggc acgcagcttt tatctgtgcg aactggcttt ggacttcgcc tcgcaacacc
    16561 tgaaaaccgg cggcagcttt ttggtcaaag tctttcaggg tgcaggctat caggaataca
    16621 tggcagccat gcgcgaaatt ttcggcactg tgcagacgcg caaacccgaa gcctcgcgca
    16681 atcgctccag tgagatttat ttattgggca aaaataaacg ctgacaatac agacggcgtg
    16741 ctttacaatc atttccgttt tacacctcaa ttatggagcc ttgctaagtg gggaacacct
    16801 ttaaatcaat ccttgtctgg gtcgccttgg gtatcggcct gatggctgcg ttcaacgctt
    16861 tggacggcaa aaaagaagac aacgggcaaa tcgaatattc tcagttcatc cgacaggtca
    16921 acaacggcga agtatccggc gtcaacatcg aaggatccgt cgtcagcggc tacctgatta
    16981 agggcgagcg caccgacaaa agcaccttct ttaccaacgc gcctttggac gacaacctga
    17041 ttaaaacact gctcgacaaa aacgtccgcg taaaagtaac gccggaagaa aaaccgagcg
    17101 cgctggctgc cctgttttac agcctgctgc ccgtcctgct gctgattggc gcgtggttct
    17161 actttatgcg tatgcagacg ggcggcggcg gaaaaggcgg cgcattctca ttcggcaaaa
    17221 gccgcgcacg cctgctggac aaagatgcca acaaagtgac ctttgccgat gtcgccggct
    17281 gcgacgaagc caaagaagaa gtgcaggaaa tcgtcgatta cctcaaagca ccgaaccgct
    17341 atcaaagctt gggcgggcgc gtgccgcgcg gcatcctgct ggcgggcagc ccgggtacgg
    17401 gtaagacgct tttggcaaaa gccattgcag gcgaagccgg cgtgccgttc ttcagcattt
    17461 ccggttccga ctttgtcgaa atgttcgtcg gtgttggtgc gagccgcgtc cgcgatatgt
    17521 tcgagcaggc gaagaaaaac gccccctgca tcatctttat tgatgaaatc gacgcagtcg
    17581 gccgccaacg cggcgcaggt ttgggcggcg gcaatgatga gcgcgagcaa acattaaacc
    17641 aattattggt tgaaatggac ggttttgaga gcaatcagac tgtaattgtg attgcggcaa
    17701 ccaaccgccc cgacgtactc gatcctgcgc tgcaacgccc cggccgcttc gaccgccaag
    17761 tcgtcgtccc cctgccggac atccgggggc gcgaacagat tttgaacgtc cattctaaaa
    17821 aagtgccttt ggacgaatct gtggatttat tgtccctcgc gcgcggcacg cccggttttt
    17881 ccggcgcgga tttggcgaac ctggtcaacg aagccgccct gtttgccggc cgccgcaaca
    17941 aagtcaaagt cgatcaaagc gattttgaag acgccaaaga caaaatctat atgggtccgg
    18001 aacgccgcag tatggtgatg cacgaagacg aaaaacgtgc gacggcgtat catgagtccg
    18061 gacacgcgat tgttgccgaa agcctgccct ttaccgaccc cgtccacaaa gtaaccatta
    18121 tgccgcgcgg acgtgcgctg ggtctgactt ggcagcttcc ggagcgcgac cgcatcagta
    18181 tgtataaaga tcagatgttg agccagctct ccatcctgtt cggcggacgg attgccgaag
    18241 acatcttcgt cggacgcatc tccaccggtg catcaaacga ctttgaacgc gcaacccaaa
    18301 tggcacgcga aatggtaacg cgctacggca tgagcgacaa aatgggcgtg atggtttatg
    18361 cggaaaacga aggcgaagtc ttcttggggc gcagcgtaac ccgttcgcaa aacatttccg
    18421 agaaaaccca gcaggacatc gacgcggaaa tccgccggat tttggacgag cagtatcagg
    18481 ttgcctacaa aatcctcgat gaaaaccgcg acaagatgga aacgatgtgc aaagccctga
    18541 tggaatggga aaccatcgac cgcgatcagg tactggaaat tatggcgggc aaacaaccca
    18601 gcccgcccaa ggattacagc cacaacctgc gcgagaatgc ggacgcggcg gaagataacg
    18661 cgccgcacgc tccgactcgg gaaaaaaccg aagcacctgc cccggcagac accgcctcga
    18721 cagagtccgg gcagcagcct gaaaacaagg cttaacttcc cgaacaaacg gcagcccgca
    18781 agctgccgtt ttcccatccc gttttcagac ggcattttcc gctcaatgcc gtctgaaacg
    18841 ccaaagccgt atcggttttt gcgaatctgc gatatggcgg tttaaattga aatcagaacg
    18901 gggcaaggct gtaccggttt aaaattaaac cgctataata ccgcccttcg aatacacgcc
    18961 cgcattggtg atgcccgtga aaaaagttga aaaaaacatc ttggtgctgc acggcgcgga
    19021 caaaatgttc gagctggtcg acaaggttga ggattacccg cactttctgc cgtggtacag
    19081 caagaccgag gtcatcgggc gtagcggcaa cgaactgaag gcgcggctgt ttatggatta
    19141 tatgcgcgtt cgccaatcgt ttgccacgca caaccgcaac atccccggca gggaaatccg
    19201 tatggaactg ctcgaaggcc cgttcaaaac cttacgtgga acgtggaaat ttatcgattt
    19261 gggcgacgat atgtgcaaaa tcgaattcaa tttggaatac gatttttcca atgccgtttt
    19321 gtccgcctta atttcccccg tcttcaacca cctttccgcc acgctggtcg aagcgttcgt
    19381 caaagaggca gaccgccgtt atgcttgaaa ttgagattgt gtacggtctg cccgaccgac
    19441 aggttttgaa aaccatgcag cttgccgagg gaacaaccgt ccgcaccgcc gcactgcaaa
    19501 gcggtttgga cggcatattt gaaaatttaa acctgcattc cgcgcctttg ggcattttcg
    19561 gcaaagccgt caaagacgac acgccgctgc gcgacggcga ccgcatcgaa gtgtaccgcc
    19621 cgctgttgat cgaccccaaa gaagcgcgcc gcaaacgcgt tcaaaatcaa gaagaataac
    19681 catgccgtct gaagccttca gacggcattc gacagaaaca cggaaaatat catgtcaaac
    19741 acaatcaaaa tggttgtcgg cttgggcaac ccgggcaaag aatacgaaca gacccgccac
    19801 aatgcgggct tttggttcct cgacgaactg gcgtggaaat ggaaggcttc gtttaaagaa
    19861 gaaaaaaaat tcttcggcga agttgcccgc gccgccctgc ccgacggcga tgtttggctg
    19921 ctcaaaccgg ccacgttcat gaaccgttcc ggacaggcgg ttgccgcgct tgcacagttc
    19981 tacaaaatca aacccgaaga aatcctcgtc gtccacgacg aactcgacat cccttgcgga
    20041 cggatcaaat tcaaactcgg cggcggcaac ggcggacaca acggcttgaa agacattcag
    20101 gcaaaactcg gcacggcaga ctattaccgc ctgcgcctcg gcatcggcca ccccggcgac
    20161 cgcaacctcg tcgtcggcta cgtcttgaac aaaccgagcg cggaacaccg ccggcaaatc
    20221 gacgatgccg tcgccaaatc cctgcaggcc gtacccgaca tcatttccgg caaatgggaa
    20281 gaggcaacgc gcttcctgca cagcaaataa tccaatgccg tcttaagccc tttcagacgg
    20341 cattttcccg atttccgtat ccgaacagtc atgaccgaac tcaagcagct tatccaaacc
    20401 gaatccatcc ccgtcatcga agaaaccctc gatttcctgc tgtacgaatg cagcatcgac
    20461 gacgcaccgt ccgccgaaga agtggcacaa tggcgcgaca tacttgccgc acgcggcggc
    20521 aaattcctgc gcctgtccaa aatctgccaa acgtggctgg acgaggaggc ggcatgaagc
    20581 tgccgcgcaa ccgcttcagc ctgctttccg cattgtggtt tgccggcggc atctattcgc
    20641 tgctcttcaa agctgccgac accgcgccgc cgccgtttcc acatttcgac aaagcagcac
    20701 accttgccct gtttttcgca caaatcttgc ttctggccaa agcattcaaa accggaaaac
    20761 ttcccatccc ctaccgcagc ctgattgcgt tcgccttctg ttttgccgtc ggcagcgaat
    20821 gcgcgcaggc atggtttact gcaacgcgaa ccggcagttt gggcgatgtc cttgccgacc
    20881 tgacgggcgc agcccttgcc ctctttgccg cgcgttctgc ctgccgcccg gactaaatcg
    20941 gtttatttct cccaaacagg atgaatcccc ctgatgcatc ctgtttttcc ccttacatat
    21001 caatcccatc tcataaacaa aaccgacaaa tcgtttacaa tatatttaca ctacaccgaa
    21061 ttacaaatat actcgaagcg gttgcagaac cgacggtttc gcaaaccgtt tcggattgca
    21121 cgacacaaaa gaaaaaccga ttttgttgcc ttaaaggagc attcatgaac ctgcatgcaa
    21181 aggacaaaac ccagcatccc gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg
    21241 actttaaggg tttgctgacc accattattt ccgccgtcgt ctgtttcggc atttaccaca
    21301 tcctgcctta cagccccgat gccaataaag gtatcgcgct gctgattttc gttgccgcac
    21361 tttggtttac cgaagccgtc cacattacca taaccgcact gatggtgccg attctcgccg
    21421 tcgtactcgg tttccccgac atggacatca aaaaggcgat ggctgatttt tccaacccga
    21481 ttatctacat ttttttcggc ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg
    21541 accgtaaaat cgccgtcagc ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt
    21601 tgatgttgtt cctcgttact gcctttctgt ccatgtggat cagcaacacc gccaccgccg
    21661 cgatgatgct gcctctagca atgggtatgc tgagccacct cgaccgggaa aaagaacaca
    21721 aaacctacgt cttcctcctg ctcggcatcg cctattgcgc cagcatcggc ggcttgggca
    21781 cgctcgtcgg ctcgccgccc aacctgattg ccgccaaagc cctaaatctg gacttcgtcg
    21841 gctggatgaa gctcggcctg ccgatgatgc tgttgattct gcccttgatg ctgctctccc
    21901 tgtacgtcat cctcaaacct aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc
    21961 cttggacgct gcaccgcgtc atcgcgctgt tgattttcct tgccacagcc gccgcgtgga
    22021 tattcggctc caaaatcaaa accgccttcg gcatttccaa tcccgacacc gttatcgccc
    22081 tgagtgccgc cgtcgccgtc gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca
    22141 ataccgactg gggcgtgttg atgctcttcg gcggcggcat cagcctgagc acgctgttga
    22201 aaacatccgg cgcgtccgaa gccttgggac agcaggttgc cgccaccttt tccggcgcgc
    22261 ccgcattttt ggtgatactc atcgtcgccg ccttcattat ttttctgacc gagttcacca
    22321 gcaacaccgc ctccgccgcc ctgcttgtac cgattttctc cggcatcgct atgcagatgg
    22381 ggctgcccga acaagtcttg gtattcgtca tcggcatcgc cgcatcttgc gccttcatgc
    22441 tgccggttgc cacgccgcct aacgcgattg tgttcggcac gggcttaatc aaacaacgag
    22501 aaatgatgaa tgtcggcata ctgctgaaca tcctatgcgt agtattggtt gctctgtggg
    22561 cttatgctgt actgatgtaa accatcgacc taaacaacaa gaccgtctga aagaatattt
    22621 ttcagacgac cttgaagttt gtcgttcaga cacaatttgt cgaataattc aaaaccggat
    22681 tctaacgaaa ggaaaaccca tgattatcct gcacaccaac aaaggcgaca tcaaaatcga
    22741 actcgatttc gacaaagccc ctgttaccgc caaaaacttc gagcaatacg tcaaagacgg
    22801 cttctacgac ggcgtaatct tccaccgcgt catcaaaggc ttcatgattc aaggcggcgg
    22861 catggatgaa aacatgaacg aaaaagaaac ccgcgatccg attcaaaacg aagcgtccaa
    22921 cggcctgccc aacgataaat acaccatcgc catggcgcgc acttccgacc cccattccgc
    22981 cggcgcgcaa ttcttcatca acactgccga caacgctttc ctaaacttcc gttctaaaga
    23041 gctgtacggc aaaaccgtcg tccaagactg gggctatgcc gtattcggta aagtcgttga
    23101 cggttttgac gttgtcgatg ccatcgaaag cgtatccacc aaacgccacg gctaccacga
    23161 cgatgtgccg accgaacctg tcatcatcat taaagccgaa gcagtataaa ccggcaatcc
    23221 gaaagcagcc tgcataaaag gctgctttta tttcagacgg catgttattt gatgtaaccg
    23281 ggctatgccg tctgaaaacc ggacgcaagc cttcagacgg cataccgcta tgttaaaata
    23341 cgcccgtttt tcctaaacgg acacaaagga aaccttatgg caagcatcac ccgcgacatc
    23401 ttcaaagcct acgacatccg tggcatcgtc ggcaaaaccc tgaccgacga tgccgcttat
    23461 ttcatcggca gggccatcgc cgccaaagcc gccgaaaaag gtatcgcccg catcgcgctc
    23521 ggacgcgacg gacgcttgag cggccccgaa ctgatggagc acatccaacg cggcctgacc
    23581 gacagcggta tcggcgtact caatgtcggc atggttgcca ctcctatgct ctacttcgca
    23641 gccgtcaacg aatgcggcgg cagcggagtg atgattaccg gcagccacaa tccgcccgat
    23701 tacaacggtt tcaaaatgat gctcggcggc gacacgctcg caggcgaagc cattcaagaa
    23761 cttttagcta ttgttgagaa agacggtttt gttgccgccg acaaacaagg cagcgtaacc
    23821 gaaaaagaca tctccggcgc ataccacgac cacatcgtcg gacacgtcaa actcaaacgc
    23881 ccgataaaca tcgccatcga cgcgggcaac ggcgtgggcg gcgcgtttgc cggcaaactc
    23941 tacaaaggtt tgggcaacga agtgaccgaa cttttctgcg aagtggacgg caatttccct
    24001 aatcaccacc ctgatccttc caaaccggaa aacctgcaag atttgattgc cgcgctgaaa
    24061 aacggcgatg ccgaaatcgg cttggcgttt gacggcgatg ccgaccgctt gggcgtggtt
    24121 accaaagacg gcaacattat ttatcccgac cgccaactga tgctgttcgc ccaagacgtt
    24181 ttgaaccgca atcccggcgc gaaagtcatt ttcgatgtca aatccacacg cctgcttgcc
    24241 ccgtggatta aagaacacgg cggagaagcc ataatggaaa aaaccggcca cagcttcatc
    24301 aaatccgcta tgaaaaaaac cggtgcactg gttgccggcg aaatgagcgg acacgttttc
    24361 tttaaagaac gctggttcgg cttcgacgac ggcctgtatg ccggcgcacg cctcttggaa
    24421 atcctgtccg cctccgacaa tccgtccgaa gtgttggaca acctgccgca aagcatttcc
    24481 acgcccgaac tcaacatctc cctgcccgaa ggcagcaacg ggcatcaagt tatcgaagaa
    24541 ctcgccgcca aagcccaatt tgaaggcgca accgaaatca tcaccatcga cggcctgcgc
    24601 gttgaatttc ccgacggctt cggtctgatg cgtgcttcca ataccacgcc gattttggtg
    24661 ttgcgttttg aagcggatac gcaagcagcc atcgagcgca ttcaaaaccg attcaaagcc
    24721 gtcatcgaaa gcaatccgca tttaatctgg cctctgtaaa aatagaaaaa atgccgtctg
    24781 aaactttcag acggcatttt tcacaagtag ccaatcaaat cttggcagat tggatttggc
    24841 ttaaaaatct ccgcgtccgt tcgtgtttga ggtggtcgaa caactctttc gggctgccct
    24901 gctctacgat aacgccgccg tccatcacga cgacgttcgt ggcaacttcc agcgtgaact
    24961 tgatttcgtg ggtaacgacg accatcgtcc aaccttcccg cgccaattcc ttcatggcgt
    25021 ccaacacgtc ttgcaccaac tcggggtcca gcgcggaagt gggttcgtca aacagcatca
    25081 attcaggctg aatcgccagt gcgcgggcga taccgacacg ctgctgctga ccgccggaaa
    25141 gctggtaggg atacaaatcc actttatcgc ccaagccgac tttttccagc agtttcaaag
    25201 cctcttcgcg cgcttgggcg gcaggcttgc cctgtacggc aaccggccct tccatcacgt
    25261 tttccaacac ggttttatgc gggaagaggt tgtattgttg gaataccatt ccggacttgc
    25321 ggcgcagtgc caaaatatcg tgtttgcttg tttttttgga aaaatcaatg cgtaacggcc
    25381 gcgcgttgtc gaactcgatt tgtccgtctt cgggcatttc caacgcgttt aggcagcgca
    25441 gaaatgttgt tttacccgag ccggaaggcc cgaggatgac gaccacctgc cctttgccca
    25501 catccaaatc gatgccgcgc aaaatggtgt tttcgccaaa ggtcttatgg atattgcgga
    25561 ttttaatcat gacaactcct tatttggcga cataacggtc gaaacgtttt tccaaacgcg
    25621 cctgaatcaa aaacagcact ttacagaaac accaataaac caatgcagct tcgatataga
    25681 caggcaaaaa gtcataagtg cggtttgccg tttcctgtgc gacacggaaa agctccgtta
    25741 ccgttaccac ggcggcaagc gaggtgtttt tgaacaagcc gataaattcg ttgctcaacg
    25801 gcggaacggc gacgcggaat gcctgcggtg cgacgatgcg gcggaacgtc tgcatatagg
    25861 tcataccgat ggagaaacct gcttcccact gccctttcgg cacggacaaa atcgccgcgc
    25921 gtatggtttc ggaagcgtat gcgccgacat tgagcgaaaa gccgatgatg gcggcgggaa
    25981 tcggattgat atagatgccg acggacggca gcccgtaaaa cacaatcaca agctgaacca
    26041 acagcggcgt accgcgaacg acggaaatat aaaattccac cagcttcaac aagcattttt
    26101 ggaaaatacc gccggaaggc atgattctta ccaaagcaac ggctacggca ataatcatgc
    26161 cgataacgaa agaagcgatc gccaaaggca aagacactgt aaagccggct ttaaccatgg
    26221 gccaaaacgc gctgatgagc atatcagcgc gtgtttccgt cataaacggc agagaggcaa
    26281 ggaaattatt gaacactgat gtcttttccg aagaattgtt cgcccagttt tttcaacgtg
    26341 ccgtcggctt tcagctcgtt gattgccgtg ctgaatttcg ccacggcctc gtcattgccc
    26401 ttgttgacaa tcagaccgga accgactttt tcatcggcag gcgcggacca cacgattttc
    26461 acccccgcat tcgggttttt cttcagatag tccaaaaccg ccaattcatc gttcaacgtc
    26521 gcatcggcgc gtttttgttc aatcagggtc agcgattgcg ccaaaccgtc caccggcacg
    26581 agttgcgcac ctgcggcttt ggctttttcg ccgtagttgc tggtcaggga ttgcgcggtt
    26641 ttcacgcctt tgatgtcggc tatggattta atgttgctgt cgttatgcgc aaccaaaacc
    26701 gcaccgctcc agctgtaagg ttcggatttg tcaaatgtcg cctggcgttc ggggctggtc
    26761 aggccgactt ggtttgccac cacgtcgaaa cgtccggctt tcaaacccgc catcatcgaa
    26821 tcccattgcg tttctttaaa ctcgactttt acgcccagtt tttccgccac ggcgcgggtt
    26881 acttccacgt cgtaaccggt cagtttgccg tctttgtcgt ggtaggtaaa cggtgcgtaa
    26941 gtgccttccg tgccgacggt aaccgtgcct ttattgttga tgcgctcgat taaagaaccg
    27001 gaaattgccg attgtgcagg cgcggaagat gctccgctgc cgccttccga accgccgcag
    27061 gccgccaaaa ccaatgcggc aatgccgccg agtacgaatt ttttcaacat cattccctcc
    27121 tgtaaaaagt gtgcaagtgc ggcattctaa gtgaaattgc ggcaaacaca aagaatactt
    27181 tttttatatg gttattccaa atcggaatgg gaaagatatg ccgaagccgc cggaagcgga
    27241 aataaagggg gcgggaaagg cggcgggcgg cacgaaaccg aaaagcggca tcaggtatgc
    27301 aaacggcgga ttcacgcacg ccgttccggc aaaaatccgc ctgccgattc ggtgctataa
    27361 tccacccgaa acagatgaca ccaaaaagga acatctcatg aacacgcccc aatccgccat
    27421 cattcccgac cacgcccaag ccggcatctt tatcgaagcc gacttcgccg ccaaccgcct
    27481 caacgatatt aaagccgcct gccgcgcttc gctcgacgcg ttatccgcct tgaaagcccg
    27541 ctttccggac gatattttgg gtctgaccat cgccttcggc agcaaagctt gggcaacatt
    27601 cggacacacg gacgaaggca gtgaaatcaa acccttcccc gaaatgggca acgggcttgc
    27661 gccgtccacg cagcacgata tgtccatcca catccaatcc ttccgccaaa acgcagccta
    27721 cgcgcttgcc caatccgttt tgggcgcgtt cggcgacagc atatgcgtcg cgtccgaaga
    27781 acacggtttg cgcctgtatc aggatcgcgg gctggacggt ttcgtcgacg gcacggaaaa
    27841 cccgcaaggc gatgaaacaa tccgcgaagt cgccatcatc cccgaagggc tgcccgatgc
    27901 aggcggcagc tatgtcctgc tgcaaaaata cctgcacgat ttgaaaaaat gggatgccgt
    27961 ccccgtcgcc gaacaggagg cctcggtcgg acgcagcaag gaaaccgacg acgaattcag
    28021 ccgcgatgtc cgcctgcccg attcgcacct cggccgcgtc aacctgaaag aaaacggagt
    28081 cggcctgaaa atcgtccgcc gcagcctgcc cttcggcaaa atcagcggcg aacacggttt
    28141 gatgtttacc gcatactgcc gcacgctgca taatatcgaa gcgcaattat tgagtatgtt
    28201 cggcgacacg gacggcaaaa ccgacctgct gctccgacac ctctccgccg ccgtttcggg
    28261 cggatactac tacgcgccgt ctgtcgaacg cctgcaaaac ctctaagccg gacgatgccg
    28321 tctgaaaccc tttttcagac ggcattttcc cgttttaccg tccaaccgtt ttcatacacg
    28381 ctatgtccgc acccatccga gcattcgacc cgctgaccgc ccccatttcc ggcaccaacc
    28441 tgattgaggc ctctgccggc accggcaaaa cctacggcat tgccgccctg tttacgcgcc
    28501 tgatcgtatt ggaacaaaaa agcgtcgagc gcgtattggt cgttaccttc accaaagccg
    28561 ccactgccga gctgaaaaca cgcctgcgcg cgcgtttgga cgatgtgttg caggttttgg
    28621 aaagcaaaga aattgccgaa cttggagacg acacgctttc agacggcatt gccgcctact
    28681 gcgccgaaca ccacgaaggc gacaccttcc tgcccgcact cttggaacag gctttgcaaa
    28741 aagaaagccg gacgcgtctg attgtccgcc tcaaggccgc catcgggcaa ttcgacaacg
    28801 ccgccatcta caccatccac ggcttctgcc agcgcatcct gcgcgactac gccttcctgt
    28861 gccaagcacc gttcgatgtc gaactgaccg aagaagacgg cgaccgcctg cttgtcccgg
    28921 ctcaagattt ttggcgggaa cgcgtcagcg gcgacccggt gcttgccgca ttggcgttta
    28981 aacgcaaagc tgtgccgcaa accgtccttg cccaaatccg cgcctacctg tcccgcccgt
    29041 acctgaattt ccgccgtccg caggcggatt tgaaacaggc gcagcgcgac gccgaaacct
    29101 catggcaaac cgtctgccgc ctgctgcccg agctggaagc cggcttttgg cgcatccatc
    29161 ccgacctcaa cggcaacagt taccgcaaaa acagcttcgg caaccttttt aaagaactgg
    29221 cacaaaaatc cgccgccgga caactgccct ttctggacaa agacacgcac gaacggctct
    29281 tgaaactctc atccgacaaa ctcgaagccg gactgaaaaa aggcaaaacg cccgatgcgg
    29341 cagtatttgc cgaattgcag aaactggcag acttcgggcg cgatttgaac gcactcgaag
    29401 aagcggaaga aacaacaatg atccggctgc aactggattt aatcgaatat ctcaacagca
    29461 gccttgccga aatgaaaaaa tcgcgccgcg aacgcggttt cgacgacctg ctgctcgatg
    29521 tccacaccgc gctgaccgac aatccgcacg ccgaaacccc cgcccgcgcc gttgccgaaa
    29581 actgggaaat cgcgctgatc gacgagttcc aagacaccga cccgctgcaa tacgaaatct
    29641 tccaaaagat tttcatcgcc cgaaaccgcc cgctgttcct cgtcggcgac cccaaacagg
    29701 cgatttacag ctttcgcggt gcggacattt acgcctacct tcaggcggcg gaagacgcgc
    29761 ggcaccgcta cacgctcgcc accaactacc gcagccacgc cgcgcttatc ggcagcatcg
    29821 gcgcgctgtt ccgcctcaaa gaacgcccgt tcgttttgga aaacatcggc tattcggaag
    29881 tcggtgcggc gcgtgccgaa agcaggttgt cgcccaaacg tcctgccgta cagctccgtt
    29941 ggctgcacga gaacgacaat gaaaaagcca acaaagacgt tttgcgccgc cgtgccgccg
    30001 actattgcgc cgacgaaatc gcccgcgcgc tcaacgaagc ctccggaggc cgtctgaatt
    30061 tcaaaggccg cccgttgcag tcgggcgata ttgccgttct ggtacgcacg cacaacgagg
    30121 cggtgatgat ttccgccgca ctgaaaaaac ggcaggtgca aagcgtcctg ctttcgcgcg
    30181 aatccgtgtt cgcctcgccc gaagccgccg ccctgtccgc gctcatcggc ttctggctcg
    30241 aaccgcgccg cgccggaacg ctgcgctttg tcctgacaag cggcatattc ggttatgacg
    30301 cgcagcaatt gcacgacttc aaccaaaacg aaagcgagat tttgcattgg gcggaatctg
    30361 cccgaaccgc gctcgacata tggcaaaaat acggcatttt cgccgctatg cagcagtttt
    30421 cccaaacaca cggcatcgaa acgcgcctct taagccggaa caacgggcgc agcctgacca
    30481 attatttcca actgctcgaa ctgcttgccg ccgaagacgc gcaaaaccgc aaccccgccg
    30541 cactgcacaa atggctgcgc gatcaaatca gcctggccgg caacaacggc ggcgacaacc
    30601 gcgccatccg tttggaaagc gacgaagatt tggtcaaaat cgttaccatg cacgcctcga
    30661 aaggtttgca gtatccgctg gtgtactgcc cgtttgcgtg ggacgcgcaa gataccggac
    30721 cgtccgactg gcaaatcctc caccaaggcg caaaccgggc cgaacttttg gcaaaggcgc
    30781 aactgtcgga aagcgaacag aagcaatacg ccgacgaaga aatggcggaa cgcctgcgcc
    30841 tgctttatgt cgcgctgacg cgtgccgagg aacagctcaa catctacgcc gcctattctt
    30901 ccgataccgc cgacaacccc cttgcctacc tgatcgaagg cttgccgcaa gacagccgcg
    30961 aaaccgtccg ccgtacctat gcgtgtgaaa aagacggcat aacgatgctc aaacgcaact
    31021 ggcggcgcgt ggcggacaat gccccggccg gcacggattt cgccttcaca gaagatgcgc
    31081 cgccccctgc cgcctatcgc ggcaacgccg atcaagccgc cgaatttgcc gcaaacagca
    31141 ttcccgaacg cggattccga tttgtccgcc acaccagctt taccgcctta agccgccata
    31201 cccaaacgcc cgacggcggc gaagaagatg cctgcccgtc cttggatgcc gccgaaacct
    31261 cggtgccggc gatgccgtct gaaacgccga cggcttcaga cggcatatcg atacacgact
    31321 ttccgaaagg cacgcaggcg gggctgtgcc tgcacgaaat tcttgaagat ttcaaattcg
    31381 gacaagcagc cgccgaacag gaaaccctca ttgccgacaa gctgaaaaaa tacggttttg
    31441 aagaaatatg gctgcccgcc gttgccgaaa tggcggaagc ctgccgcaaa acgccgctga
    31501 ctggggcata cggcctgtcc gacatcccgc ccgggtgccg ccgtcccgaa atgggcttta
    31561 ccctccacac cgaagacttc ggcctcaaac ggctgcgcga ctggcttgcc cgcgacgaca
    31621 tcaggctgcc cgaagtctgc cgtgccgccg ccgagacgct cgacttccac accgtcaacg
    31681 gctttttaaa cggctttatc gatatggtct gccaagaccc cgacggcaat atctgcatca
    31741 tcgactacaa atccaaccac ctatccgcct acacgcggca ggcgatggac gaagccgtcg
    31801 cgcaccagca ctattacctt caggcactga tttacgccgt tgccgccgcg cgctacttca
    31861 aactgcgcgg acaaccgccc gccgccgttt ccgtccgcta cctgtttttg cgcggattgg
    31921 atggcaaagg cggcggcgtt tggcgttggg acatagatgc cgccgctttg gaacagataa
    31981 aataacttcc cgtcccttaa gacgacaacc gcaggagaac cctatgaata tcaaacaatt
    32041 gattaccgcc gcactcattg cctcagccgc ctttgccacg caggcagccc cgcaaaaacc
    32101 cgtatccgcc gcccaaaccg cgcaacattc agccgtttgg atcgatgtcc gttccgaaca
    32161 ggaatttagc gaaggtcatt tgcacaacgc ggtcaacatc cccgtcgacc aaatcgtccg
    32221 ccgcatatac gaagccgcgc ccgacaaaga cacgccggtc aacctctact gccgcagcgg
    32281 acggcgtgcc gaagccgccc ttcaagagct gaaaaaagca ggttatacaa atgttgccaa
    32341 tcacggcggt tatgaagacc tgctcaaaaa agggatgaaa tgacgcaatg ccgtctgaaa
    32401 ggacaggctt tcagacggca tacggcgttc cggcacgcgg cacggcatca ggcgcggcgc
    32461 gtgccgcccc gtccgaagcc cgataaacaa accgataagg aaatacgatg aaacacatac
    32521 tccccctgat cgccgcatcc gcactctgca tttcaaccgc ttcggcacac cctgccgaca
    32581 aaccgcccac ccaaaacgaa accgctatga ccacgcacac cctcacctcg aaatacagtt
    32641 ttgacgaaac cgtcagccgc cttgaaaccg ccataaaaag caaagggatg gacatttttg
    32701 ccgtcatcga ccatcaggaa gcggcacgcc gaaacggcct gaccatgcag ccggcaaaag
    32761 tcatcgtctt cggcacgccc aaggccggta cgccgctgat ggtcaaagac cccgccttcg
    32821 ccctgcaact gcccctgcgc gtcctcgtta ccgaaacgga cggcaaagta cgcaccgcct
    32881 ataccgatac gcgcgccctc atcgtcggca gccgcatcag tttcgacgaa gtggcaaaca
    32941 ctttggcaaa cgccgaaaaa ctgatacaaa aaaccgtagg cgaataagcc cgtcgccgac
    33001 atatggcgga tggaaatcaa atgcacacgc ccctcattcc cccgaagtcg ggaatccaag
    33061 cggttgggtt accgtgattt ccaatcattg ccgaaacgga cgattccgga ttcccgcctt
    33121 cgcagggatg acgggcgtat gcgttttgcc atatcacagg caactttgcc gcaaatctta
    33181 tctgtcgccg gtatgccttc ttgtttcaat ccgccacaaa ccaaatgccg tctgaaacct
    33241 gatttcagac ggcatttccg tttattccct gctgtcgcgg caaatatcga tggcttcctg
    33301 caaacttgaa acgccgtgga ttttcaggtt cggaaactct ttggcattgc gcggcatatt
    33361 ggctttgggg acgatggcgc gtttgaagcc gagcttttcc gcttctttga gccgctcttg
    33421 tccgcgtgcg acggggcgga cttcgccgct taggccgatt tcaccgaaga ccacggtttt
    33481 ttcaggcata gggcggttgc ggaagctgga aagcatcgcg aggatgaccg ccaaatccgc
    33541 cgccggttcg ccgattttca cgccgccgac ggcgtttaaa aaaacatcct gatcgaaaca
    33601 ggcgatgccg ccgtgccggt ttaacacggc aagcagcatc gcaaggcggt tttgttccaa
    33661 tccgacgctg aggcgtttgg gtgtgaagcc gtgcgcgtca tcgaccaatg cctgaatttc
    33721 gaccaaaagc gggcggctgc cttcctgtgt aaccaaaacg cacgaaccgg gcgtgtcgtc
    33781 gcggtagctg gcgaggaaga tggcggacgg gttggatacg cctttcaaac cgttttcggt
    33841 catcgcgaac acgcccaatt cgtttgccgc gccgaaacgg tttttgatgg cgcgtatcat
    33901 gcggtagttg gaatgttggt cgccctcgaa atacagcacg gtatcgacca tatgttccag
    33961 cacgcgcggg ccggcaatcg ctccgtcttt ggtaacgtgt ccgaccagta tcatggcgat
    34021 acccatttgt ttcgccatac gcgtcagttg ggcggcacat tcgcgcacct gcgacacgga
    34081 accgggggcg gacgtgattt ggtcggaata catggtttgg atggaatcga taacgacgac
    34141 ttcgggctga tgctgtttca aggccgcctg aatcgcttcc atgcggattt cggcaagcag
    34201 gtttacgcct tcggcgtgca gttccaaacg ttgcgagcgc agggcgacct gttgggcgga
    34261 ttcttcgccg gaaacgtaca gcactttgcg gctttgcgcc attttggcga tggtttgcaa
    34321 caacagcgtg gatttgccga tgccgggatc gccgccgagc aggatgaccg cgccatcgac
    34381 caaaccgccg cccaatacgc ggtcgagttc gcccataccg gtcggattgc gcggcacttc
    34441 ggcagcggta acggcggaga gggattggac ggtcgaagtg tccgccgccc aagattggaa
    34501 tcgggcgttt ttcggctcgg gcgcggcaaa gctttcctga agcgtgttcc actcgccgca
    34561 atgcggacat ttgccctgcc atttcgggga agtgccgccg cattcggtgc attggtaaag
    34621 ggttttaagc gtttttgcca tgatgttttc cctatgggat ttttgaagtg taaaaaaatc
    34681 gtctgaaact tcttttctaa aaaatttttt attattagtt tgttaatttt gattacaaga
    34741 ccatatcacg atacaaacaa gggtggcttt ctgttatttt gcggcataat gacggaaaat
    34801 acaaaacagg aatcccaaac aatgactttc ccattaaata taatggtaga attgcgttgc
    34861 gttgcgttgc gttgcgttgc gttgcgttgc gttgcgttgc gttgcgttgc gttcagagca
    34921 tagttcacct ttaaaatcca tacaaggatt tttatcaaat ttatgcatag gagtgaaatc
    34981 ctatgctatc taaacaaatc tcaaatctta attcttctag taacaaacca aaaatcctat
    35041 ctctattttc aggatgtggc ggtttggatt tgggctttca tcaagctggt tgtgaaactg
    35101 tttgggcgaa cgatttctcc cattgggctt gcgaaagttt ccgtaaaaat atcggcgatg
    35161 tcatcgtaga aggtgatatt gaacaaatta atccgaatga tccaactatt cccgattgcg
    35221 acatcatttt aggcggattc ccttgtcaag atttttccat gatttggaaa cagccgggct
    35281 tagagggtga gcgcggcaat ctttataaaa gctttttacg ttttgtaaat gcaaaaaaac
    35341 cgaaagtttt tgttgctgag aatgtgaaag gtttattgac tgccaacaag aaaaaagcca
    35401 tccagcaaat tattaccgac tttgaaaatt gcggttatta cgttcaggcg aagctgtata
    35461 actttgcaga atttggcgta cctcaatttc gtgaacgtgt gctgattgtc ggagtacgtt
    35521 tggatacagg atttgatttt cgccatccgg aaccgacgca caatgaaact ggcgaaaacg
    35581 gcttaaaacc atatgtaaca gcaggtcagg ccatatccaa tattccacaa aatgccagta
    35641 ataatgaatt actgaaaatc agcgataaaa cacgccgtat gttggaatta attcctgaag
    35701 gtggaaattt taccgatatt cctaaagatc atcctttata tgtgaaaggt atgattagcc
    35761 acgtttatcg tcgtatgcat cggaacgagc catcaaaaac aattattgca gcaggtggcg
    35821 gtggtacttg gggctatcac ttccctgaac cgcgtgcttt tactaataga gaacgagcaa
    35881 ggcttcaaag ttttcctgat gattttgagt ttgtcggatc aacaactgaa gtacgtcgcc
    35941 agattggtaa tgctgttcct cctcagggcg tggttgaact ggcaaaaagc attttaccga
    36001 ttttttcaga caactatgag aaagtagatt tgcatgagaa attagtcgaa gaaaaagaaa
    36061 ttttattcca tgaccgacta agcaaaattc gagggggaaa acaatgaata cagttttttc
    36121 caatatcgcc aatgccaaaa tcactgaaaa atctcttaat gcggtttgga tggatttatt
    36181 taaatcagcg gatgaagtgt tgatggcgac aggttatgta tctaatgacg cagtggttga
    36241 gttacataaa attttggagc taaacgatca tattcaaaaa atagatttat tggtcggaat
    36301 gcattatttg gaaggtttta gccatttaca atatgacagc ttatgtaaac taaatgattt
    36361 tttgcgacat gagaaaagag gagccgtcta tgtttccccc tttgtgaaat ttcacggcaa
    36421 aatgtattcc tttaaaaatt atcaaaaaat caatggatta attggttctg ctaacttaac
    36481 gtgtttttgg gatagcacgg aacgtactta tgaaactatg ttgcatttga acggtaagcc
    36541 tgcacagatt ttgcaggcag atattcaaag tactattcat aagctaggca aaaatatcca
    36601 agaagttgaa agaccaagta aatttattga acataatagc catttagaaa attgtttggg
    36661 tgttcagaaa attgcaccag aacagattcg gcaattattt gcccaaacgt cagaatatca
    36721 tttctcaatt ccagctaaaa ccgaagaaaa aagtaatttg aatgtatttt ttggagaagg
    36781 tcgacgagat aaacgagggt ttgtcaaacc acgaccttgg tatgaagtag agttgattgt
    36841 atccaaagac atcacttcac aagaagggta tccggttctg aagtctttta cagttattac
    36901 cgatgatggt tggcaatttc aatgcaagac atcgggggat tactccaaaa acttccgctc
    36961 agaaaatgat ttaaagacac ttggtaaatg gattaaaggt cgtttggaaa gtcatggctg
    37021 cctgcaaaat aacgagaaga ttacgcatga aaccctacgc gaatacggta acgatcattt
    37081 tgagttacgt tcaaccgata atcctgatgt ttggctgctc tcatttaagg ggaaaaacta
    37141 aatgttgcga acctacttga accaattgac ccctcccgaa ttagctgatt ctgttaaaaa
    37201 taccgttgat ggttttatgg aaaaattgtc acaaaccgag ccgaaaatcg cccaaaatgt
    37261 tttattgctg ggcaatgttc aaagtggcaa aacggcacag gttttgggtg tattaagtgc
    37321 gctggctgat gatggcgacc acaaagtttt tttgtatctt actactgaca gcgtagattt
    37381 acaggatcaa acagtcaaac gagccaaagc caatctgaaa aactttatcg tattgtctga
    37441 agctgacgac cgaagtttta tggaagtaat gaaggcagag aaccccatct tggtcgtgat
    37501 taaaaagaat gcccgtgtat tgaaacgttg gcgtaatttg tttgccagtc aaagtagcct
    37561 gaaaggctat cctttggtta ttgtggacga tgaagccgat gctgccagcc taaatactaa
    37621 ttctgataag ccggctaaag atgccagtac tatcaataaa ctgctaaacg acatcaaaaa
    37681 ttcctgttgt caaagcctgt ttatccagtt gactgccacg cctcaatcgc ttttattaca
    37741 acatgaagaa tctgattggc aacctgaatt tatccacttc tttgaagcag gcgaaaaata
    37801 catcggcggc aactttgtct tttctgatcc accaagctat atagttcgtt ttattgatag
    37861 cgaattggat gatatgaaag acgaaagtgg tgaaattgcc gaaggagcaa aacaggcatt
    37921 gcttagtttt ctaataacct gtgcagaatt cgcgctatgt gataaggcta actgtaattt
    37981 tgcactacat ccaagctata aaattcaaga tcatcaagcc ttttcaaaga agatccaagc
    38041 ctttttaaat gacttagtgc aagcagtcaa taatggggaa gaccttgcag gtagctttaa
    38101 agaaagttat ttggatttgc aaaagaccaa gcccgatatt catcactttg atgaaatata
    38161 cgaaaaattg acagcacttt tagagaataa acaaatctct actcttgtcg ttaactcgca
    38221 gacagaaaca gattttgact tagaaaaagg tttcaacatc attattggtg gtaatgtgat
    38281 tggtcgtggc ttgactattc cgaaactaca aacggtttat tatagtcgca ctgccaaaaa
    38341 accgaatgca gatactttct ggcaacattc gcgtattttt ggatatgacc gcgataaatc
    38401 attattacgg ctttatattc cgttcgatgt ctattacttc tttgtacaac tcaatcaagc
    38461 aaataatctg attattgggc aggcaaaaaa ttcaggcggc aatattcagg ttatttatcc
    38521 gaaaaatatc aatccaactc gaaaaaatgt attgaaattc gatagtatca atcaaattgt
    38581 gggtggtgtg aattattttc cactccaccc aaacgaagat aatttatcgg agattaacaa
    38641 aattttgcct tctattttga aagatgaaat tcaatctgat ttatatcaaa tagatataga
    38701 agatttattc ttggttctag ataaattggg tcgttatgtt ccagatgact ggaataagga
    38761 aaaatttatt gctggtgtag aagcattaaa agcacaacgt ccaagtttta aaacctatgt
    38821 tttgatcaaa accggacgta aactttctcg agcaaccggc acaatgcttt cggaagacga
    38881 ccgtaaatta ggagaaaaat atcccaatga tttattcctt acgctttatc aagtagtagg
    38941 aaataaagac aaaggttggc agggaaaaga tttttggtta cccaatatca agctaccaca
    39001 caatggcttg gtgtaccaaa gtgcaaaatg aagcatatgc cgtctgaaaa cttccgattc
    39061 ttcagacggc atatcgggcg gtttcctgcc ggcttacccg ccgtgatact gccgcgacag
    39121 ctcgtgtacg gtgtcgacta agattttggc gtgttcgggg tcggcgtgtt ggttgatgcc
    39181 gtgtccgagg ttgaagacat ggccgctgcc gtgtccgtag ccggtcagga tacgtgcgac
    39241 ctcggtgcgg atggattcgg gcgtaccgaa gagggcggac gggtcgaagt tgccttgcag
    39301 ggcgacttgg ttgccgacgc ggcggcgtgc ttcgccgatg ttgcacgtcc agtccaagcc
    39361 caatgcgtct gcgccgattt gggccatgct ttccagccac agcccgccgc ctttggcaaa
    39421 cacgataaca ggcacgcggc gaccttcgct ttcacgtttg agtccggcaa cgatttggcg
    39481 gatgtatttg aggctgaact ctttaaacgc cgcgtcgctc aacacgccgc cccaagtgtc
    39541 gaaaatctgc accgcctgcg cgcccgcgtc gatttgggcg ttgaggtagg cggtaacggc
    39601 ttgggcgttg gtatcgagga ttttgtacag caaatcgggg cgcgagtaca tcatggtttt
    39661 gatggtgcgg aattctttgc tgccgccgcc ttcgaccata taacaggcga gcgtgaacgg
    39721 gctgccggag aagccgataa gcggtacgcg gccgtccaat gctttacgga tggaagttac
    39781 cgcgtcgaaa acgtattgca gtttttccat gtcgggaacg tgcagcttgg cgatgtcgga
    39841 ttcgtgttgc aaggcgcgtt tgaatttcgg gccttcgcct tcggcaaaat acagccccaa
    39901 gcccattgca tcgggaacgg tcaagatgtc ggaaaacaaa atcgccgcgt ccaaatcgaa
    39961 gcgttccaaa ggctggatgg tgacttcggt cgccaatccg gtgtttttgc acaaatcgag
    40021 gaagctgccc gctttcgcgc gtgtggcttt gtattcgggc agataacgcc ccgcctggcg
    40081 catcatccaa atcggtgtgt attcgacggg ctgtttgagc agggcgcgga ggaaggtatc
    40141 gttttttaaa agagtcatac gggttttctg tcagtctgtt tgtcaagtga aatggttttc
    40201 agacggcatt tcaacgatgc cgtctgaagg tttcaacggg tttcggcgga agggcggttt
    40261 tccccggcaa cgcttgccaa aaccaagttg cgctgtgctt cggctttttg cgactgtgcg
    40321 gtttcgtcaa aaacctttgc caacaccaaa cgcgccggaa tactcggctt cagtgcaata
    40381 ctcgcttcaa ggtagccttt tgccttaccc caaagtttgc ggccgtaggc gagccggccg
    40441 agatacatca gcagaagcgc gttatcgggc tgttctttca gccaagaatc ggcaaaatcg
    40501 atggctttct gctgttcgcg ctcgcccaaa aagcgcacgc tttcgacaaa ggcttccaaa
    40561 agctcggggc ggcggttgtg cggataatgc tgtttgaccc atttgaccgc atcggcatac
    40621 agtcccaaac gttcgtactt ttccgcaacc gatacgctca attccccgtt tttgaggctg
    40681 tcgggaatcc gcttcaggca ggttttcaaa gcggcggcat cggcagcatc cgccatctgg
    40741 cggcggtatg cccaattttg ataccgttcc atttccgatt tgcccaacgc gcccgccttg
    40801 gaaagttttt cggtttttgc cagaacctgc aacgcatcgc cccgatcgaa ggcgtaacga
    40861 agttgcagac gcacgaggcg cgtaaggttg gcattcatct tcgccgccgc atgaagattg
    40921 gcttccgccg cttcgtaatc gcgccggttc agtgccgatt ccgccagcag aagatagcgg
    40981 gaaagctgct gtttttccgg cagtttggcg atttccgcaa gataacggtc gcgcagctcg
    41041 atattttcca tctgtcctgc cgcgtgcgcg cccagcatca atgccaaagt ccgattgtct
    41101 ccggcctctt tgttgcccaa cactcgagag gcttcgagtt ccgccttttc aaaacgccct
    41161 tcgaaatacg ccaaacctgc cttattcaag gcaagcgcgg ccttgcggcc tttccgcgcc
    41221 gaaccggaac gctgcatctt ttcggggata ttaagtacgc cgatgatgaa tttaaacagg
    41281 aaataccaca ccacgacggc aatcagcgaa cctaacacaa aagcgtgcag gttgattctg
    41341 agcatggtct gtccgagtac gatatacacg tcgccggtgt aaatgcccga agccagcgcc
    41401 agtccgacgg cggcggcaaa caggacgaca atccagacta ccgttttcat gcgcgttctc
    41461 ctttagcgga cggcgaacgc cctgcctttt ccggtgcggg ctgttttttt gcaggctgtt
    41521 ccggtttgcg ttccgaaggc agggacggtg cttctatggc cttcacatcc gatgcggcag
    41581 ccggttcgga agctgtgttt tcgtttgccg gttcggaagc cgcctgttcc gcttcttgat
    41641 tttccgccgc cgtcatgcgc gtaccgtcgc gataggcgcg gacggcattt aaactggtct
    41701 tcaagccgtc atccgccgtc atgcgtacat ccagtgtttt caattccgcc agttctttca
    41761 gccacgactg cgtggcgggc gacttggcat cgaaatactg tctgacggcg gcttcggcat
    41821 tgttcaaatc gccctgatag acttcgccgt tgcgctgcat caatgcggtg cgcgcatcca
    41881 acaggcgcag acgcaggttt tcacgcacaa aatatgcctg ttcgggagaa atcagcatgg
    41941 catcgttgtt ttccaaacgg cggatttcga ccagcccctt caatgtgccg agggattttt
    42001 cccatacgtt ctgccaccat gaagcggaaa cggcttcgtt gcgggcctgt acgcccggtt
    42061 tcaatacgcc gtccaaaatc agcggcagtc cggatacggc ggtttccaac cggtcgaggc
    42121 gcaatgccgt gccggaaata tcgacatagg gacggttttt cagttccgcc aaatcgctgc
    42181 tgaccgcctg tttgatcggc agaagctctg cctgatcgaa acgggacagg cggctgtcga
    42241 tatgctccaa cacgccgact gccgtttgga tattgccggt caacaccagc tgttgcgccg
    42301 ccagattgag tatggtctcg gtttcgtcca ccagccaatc ggcacgccct ttggtcagct
    42361 cgcgataggc tttttgcgtc atcaggattt gttcgccgtt ggctttgaca ccgctgtcca
    42421 aacggtcgag ttcggactgt atggcacttt ggcggttgag gttgtctttg agcagggcgg
    42481 cgttttccga ctcgcccaag gcggctttgt cgattttctg gttgaatgcc agctcttggt
    42541 ttttcaagac attctgtcct tggacaaaca aaaaaccact tgcacccaaa cccaatgccg
    42601 ccaataccaa ggcgcaggcc gccaaagcgt tgctgccgga ctgtttgacg ataaaaggag
    42661 cggcgggaat gtgtacttct gtttcgtttt ctttgcgtgg ggaagaggtt tcagacggca
    42721 tttcttttga tgcctgtatc tcgcgtacgg gttcggatga tttgttttca ggttcgccca
    42781 ccgttttgct ccttattggt ttgaggttcc gggaaagacc atgccgtctg aaacggaaat
    42841 ggaagaatag gaaagcgcgg cttccagcgt agggacggtt tcgaccgaac acacgccttc
    42901 gcgcttcaat gcctccgcaa tgcgcggatg atgggtaaag tatagcaagg atttgaagaa
    42961 tcgggaaaat tgcggcggaa gctgtgcaaa cagcgactgc acaagttctg tggacgtaat
    43021 ataggcggcg gcaatatttt cggtttggaa attttgaaag ttcaaaggtt tatgccgtct
    43081 gaaatagact tctgccacct ccgtccgaaa acctttctcc tgcaaggcat tcatcagaaa
    43141 atcccgcccg ccgtgtccgc gcacagacaa tacgcgcgca ccttcgggca gactgttcca
    43201 aaccggcagg cgcaaaaccg cctcgctgtc gttgccgtct tcaggtgcaa acacgcccgc
    43261 gcccgcataa cgcgccaagg cacggcggct gccttgccct acggcaatgt gcgcctttat
    43321 gccgtctgaa aggttaaggt acgggacggc ggtttcaacg gcggccggac tgacccaaaa
    43381 cacggcatcc gcacgggcat acatttccgg cagccgtttc aggcttgctt cgtcggtttc
    43441 gatttcaatc ggactcaaca cttccgcctg ccatccggca cgacggcaga cttccacatc
    43501 gtccttcgcc ctgcccgacg ggcggacaat cagcatgacg ggcttcgctg tttccatcgc
    43561 cttatccgcc ccgattaccg ggaaaaatcg tcatcgttga aattcgcctg cgcggttttc
    43621 ggcgtacagc ctacccgtgc gacttccttg cccctacgct ccacaacgac accgctgctt
    43681 tccgtatgag tcacggaatc gaagcctgtc catacggtgt acataaattc gccgttttgg
    43741 aatttcatcg ttgcccggcg tccgctgccc atgccttgcc acctgtcgga acgccccaac
    43801 aggtcagctt tgctgttgcg tatggcaatt tctttttttg ccgcactgcc gaacgaatat
    43861 tcgtaaagat tgcggttgat tttttgaact tctatgtatt tgttgttgtc cgttttacag
    43921 gaaaataccg tatccgccca cacggtttgg gcggcagccg acagaagcaa tgcggcagaa
    43981 aggaacattt ttttcatcat aatttcctta atggttgaaa ccccgccatt cggacatccg
    44041 tccttcgtgg cagcagcatc agattttatt tgaaaggggt gtaacccctt tcaaattagg
    44101 gcaacacata gggcggcgct ttatgtgtcg tcctgtgtgt tgaaacatca ttgcccgtat
    44161 cctgaaatag aaggctgtgt tttaacaatc cgttgattgc ttaagatgaa ttcacactaa
    44221 aaaacagttc tctatttcat ctattacggc gggttgtttt gtttataagg gtggtaatct
    44281 acaaattgtt actaaatagc aaggtgtgaa tgaaacggca gatataatca aaatcttctt
    44341 gttggtcaaa atacggtaac ataatgcttt ttaatttttc gcctttgggt ttacgtccat
    44401 aagaaaacag atagcggttt gctgtaatac atttagcgta atacagtttt acttctattg
    44461 gcatatcgga ctgtatgggt gttaatacgg cgacatcact atttgctaca aattcacaag
    44521 ttgaaacata gctataagta tggctacctt cgccattggt agaagtgaaa agcgtatggc
    44581 atggaaaaat atgtttttca tcaacgctat cacgagcgat ataacttcgt agcgttcttg
    44641 cttgcgtact ggcaggacgg atatagacaa ccgtgtcttt ttgccgttgt tcaaattctt
    44701 ttaatttggt tgcggcaatg ccgttttgaa tagtaaaaat ttcatcaagt cgttttgatt
    44761 gtcgaaccac aggcaaatca agcggttctt ttaatttcgg ttcggttaca cccgcagatg
    44821 gatttaaatt cacgctttca acccagcttg ggatctcatc caaagatggc acaaacagtt
    44881 gtcgtaacgt gcggttagct tccctaccgc aagcagaaaa acggtaggca ttggcaaaaa
    44941 tagcagcaca ataaaagagt ttttgttgtt ctgtcatttc tgttttggga tttaaaacag
    45001 caacattttg agctgtataa aatggtttgt tttgtaaaaa agtagataaa acagaaccac
    45061 ctaatgcaac agttaataat cctgctggat aaggttttgt atttttcaat aaatccacag
    45121 atgctgttac accattattt ttgcctgacc tgcctacaaa gttgattgtt ggattgaagc
    45181 tgctcatttt attcaaatct aattttgaac cgtaactcac atcaaaaatc tcttgcaatt
    45241 caaccattat tcatcgtctc cgaagtcgtt attgtcatcg tcttcatagc tttcattatc
    45301 caaattttca gtcggcccta cttccgctcc cagtagttga aataaagcaa attcacgcac
    45361 gacttgctca aaatctgctc gggtaatttt ggaataatca gtttccaaat aggcttcggc
    45421 aacccattca tcgttatcag ttacaaatgc tgttaccgat tctcctgtgt gaacttcgtt
    45481 gtttaaataa tgttccaacc agcgcgcttt aatgccctgc cagcggtcgt ataaatcaat
    45541 gcgtcccaag tgtttggttt tttcaaaacc gtcatcacgc cagtagccaa accacgtttt
    45601 gcgacaggct tttttcgtgc tgatttcttc ttgtgtgtcc gggtcaatca cgactgtctg
    45661 aaaatgcggt ttatgggctt caaatacgac aatgcacgtt accgtgccaa ctgggtaaaa
    45721 cagttcgctc ggcatagaca tcacggcttt taagcgatga tatttcacaa tttcgctttt
    45781 ggctttgctt ggtgcaatca cgcaactgac tggaataatg gcaatgcctg tgccaccttc
    45841 tgccaacata tccaacattt ctttgacaaa ataaagttca tgcaattcgg catcgctttt
    45901 ggattgggcg taaggtggat tgagaaagcc aacatttggg cgttttaagc ctgtttcagg
    45961 tttgggattt ttgattaaat cctgaaaatc agtcatgaaa caactgcttt ggtgtaaatt
    46021 ggctttaccg tctccgcgta aaatcatatt actggcggca agagcaaaca tttttgggtt
    46081 gttttcaatg ccaatcagtt gatatttttt agagatggct tacccaaatt gctaattcta
    46141 gcaacagatt taccgtctaa attgatacca caaggaacac aaacttgttc tttggaaatg
    46201 ccacgtttcg ttgctctcgt tcctcgtttg tgagcaggac gtggcagatt aaaatttttg
    46261 tggtgccctt tgtaagaaat cgtcgaataa gtttcatcag cttgcacaat gccgtctaac
    46321 tcaacttcat tcatcatgtt ttgaagtgcg tccaaaattt tgtgccgcca agtaaacgca
    46381 gtagcaagat tgattttgca aatttcagca catttgcgaa gtgggtattt ttcaatcata
    46441 caatgaatgt atttttccca aacttcaatg tctttttgcg tgttgtagag aatagtgcct
    46501 gtctgctcaa caaaagactt tttacaatca cgacacaaaa aacgttggtt gccacaatct
    46561 ttgccatttt tgacgaaatg tgtagattgg caatgtgggc acttcgtaac ttttcttggt
    46621 tcaatgactt ttttaacctg ttcttttgag ctgacagaag tcaaaaaagc ctgtttatcc
    46681 gtatcggaca aattttcaaa aagttgtttt aaaatgtcta attcttttgt tacgcccgac
    46741 attataaaat cccttatagt tcaatttgtt ataagggatt ttatcaaaaa ttagtgcaaa
    46801 aatcaacgga ttgttaaaac acagccaaat agaaaagaaa atgcccgttt tacggtcttc
    46861 tttccgacaa aatgccgtct gaaacgaatt tcagacggca tggtcgaaaa ttattgcgcg
    46921 gcaagcggtt tgagcaggag ggtttcgccg ctttgcacga cattttccag caaatcgatg
    46981 ttcggatgct tccccggatg ggcgcgcgcg tcggcaacat gttcggcaaa ccatatcaaa
    47041 ccctgttctg ccgcttcttt acccaatttt ccatcaaaac gttccgccaa ggcattgtac
    47101 agcttgagcg aaccgagctt gccctgaacg gcgggaatgt ggtgaaccgt tttgccttcg
    47161 gcatcctgaa catccaaacc gcccagatgg tcgattgaag gaaatgtggc gagatagtct
    47221 tggaaattca tatcaaatcc gaaaatcagt cagttagcgc gtgccgaaaa tcttgtcgcc
    47281 cgcatcgccc agaccgggaa taatatagcc gttttcgttc aaacggctat ccagcgcggc
    47341 ggtataaatc gtaacatcgg gatgcgcatc attaacagcc ttcacgccct caggggcggc
    47401 aaccaagact aaggctttga tgttgcggca acctttttct ttcaacaggt cgatggtggc
    47461 caccatcgaa ccgcctgtcg ccagcatagg atcgataatc aaagccggac gctcgtccat
    47521 actgtccaca aatttctcaa aataggaaat aggcttcagc gtttcctcgt cgcgctgcaa
    47581 accgaccacg ctgattttgg cagtcggaat caggtcgagc acgccgtcga gcatacccaa
    47641 accggcgcgc aggatgggga cgacggtcag ggttttgcct ttgatgcggt cgccttcaat
    47701 ctgtccgcac catccgtcga taaggtattt ttcaatttca aaatcacggc ttgcctcgta
    47761 tgccatcagg cgcgccaact cggtggtcag cgtacggaat ttgtaggtgc tgcaatccgc
    47821 ctccctcatc agggtcagtt tgtggcggac gagcggatgg ttgataacgt taacgttcat
    47881 cgcggtgttc cagtgtgatt ttgagacgcg tcattatact tttttttaat ttgccggaaa
    47941 agaaaatgcc gtctgaaaca cgttcagacg gcattgccga atccatcagg ctttcagcag
    48001 ctcttgcagc tcgcctgcct catacatttc catcaggatg tccgaaccgc cgacaaactc
    48061 gccgttcaca taaagttggg ggatggtcgg ccagtcgctg tattccttaa tgccttggcg
    48121 tacttcggga ttttccaata cgttgacagc aacgtaatcg gtgcagcctg ccgcgttcag
    48181 gatttgcacg gcgcgggaag agaaaccgca ttgcggaaac tgcttcgtac ctttcataaa
    48241 caatacgacg cggtgtgtcg ttactacttc tttaatttgg tcgtggatgg aagccatcgc
    48301 ttattccttt tgataaagtt gacaaaattt gtcaggtagg gcgcattata cgcaaaagcg
    48361 gaaacttccc acaatatttg ccgtatatcg cgcagtatca atgttgcgaa cggcaatatt
    48421 cccactcctc gacaatcctg ctgtccggca aatggcacag ggcatatccg ccgccgcttt
    48481 cgtctttttt tacttcccgt ctgccgcctt tggcaatgca gaatttggaa gcggaatttg
    48541 ccatgcctgt ccgaaacgtg ttggcaggca gccggcagca gcgcggacat gcatacggat
    48601 gaattgcctt catggttttc cttatcgtcg gcacagggtc agacgacccg catatcgggt
    48661 acttttttaa acagccggta aaagttttcc gtcgtatatg ccgcaacctg ttccaatgtt
    48721 tgattccgca atttggcaat atgttcggca gtatgacgca caaaagccgg ttcgttctgc
    48781 ctgcctcgtt tgggaacggg ggcaaggaac ggcgcatcgg tttccactaa aatgcggtcg
    48841 tccggaacgt atttcgccgc ctcctgaacc aagggggcgt ttttaaaggt aacgattccc
    48901 gagaaagaaa tataaagccc caaatccatg gcagcccggg caaaaccgac atcctcggaa
    48961 aaacagtgaa tcacacccga attggtttta cattctttta aaatcgccaa ggtgtccgcc
    49021 gccgcatcgc gcgtatggac gataacgggc agtccggttt gattggctgc ttcgatgtgg
    49081 tctgcaaagc gtttgtgctg ccaagacaaa tcgcctttgc accagtaata atccaaaccc
    49141 gtctcgccga tgccgaccac tttcggatgg gcggcggctt cggccatttc cgcaatggaa
    49201 aattcttcgg cttccttgct gtcgggatgt acgcctatgg tgcaatagat gtgttcgtgc
    49261 gcttcggcga cggcaaagac ttcggagaag ctttccctac tgacgctgat ggcgagtgcc
    49321 tgccccacgc cgtttgcttc catgttggac aaaacttcgg gcaggcgttc tttcaaacct
    49381 tcaaaattga ggtggcagtg cgaatcgatg atatgcatag cgttacatgg taaacgtagg
    49441 tctgctgccg ctgattgtgc tgcccagttc gacttcgatc tggtctttga ctttcaggca
    49501 gttttcgtgt tttgtgaatg ccagccccac ccctttgggt ttggaggaag tactcgcagg
    49561 attaatccag gcgaccttgg tcggcaggaa cagtttgggg aagttgagga tttctacggc
    49621 aagcagaata tcgtccccga tggaaaatac gtcgtcggtc tgcacaaaca gaccgccgtg
    49681 ttccaaaaac ggcatatagg agctgtacag cagattcatg tctttcagct gcaacgacat
    49741 catttttgcc ggaatatttt gtccgtctga cataattcac ctgttctctt tcttcaattc
    49801 caaatagttg atgagcagat gctcgatctg cattttgaca tttaaagtat gaaacccgta
    49861 aggggcaagc tgtttgagca tatcctccgc cgcaaataca ttgcgcggac ggaaacccga
    49921 tgccgtctga agcagcctgt cttcataagc gggataatag acgggtttca tgtgttgcag
    49981 gcacaagcct aaatcgacca gccatttctg catccacccg acaaatacgg cgagcggaag
    50041 tttttcctta tcgaaaagcg cggcgtaatc caaaatcttc aacaatctcg gctctgccaa
    50101 tatctccagc agctttgccc tcaattcccg aagctcgcct tcctcctgaa acagcggcgc
    50161 acccgaatgg aaagccagac gttcctcagg ctccgccaca cccctgtcgc gcagatacgc
    50221 caatgcttcc ccatgcgaag gagcgggcaa aaccttcttc cggcagcggc ttttaatggt
    50281 cggtaaaacc ttgtccgccg cgtggcttac cagcaaaaag accacttgtg gcggcggctc
    50341 ttccaacact ttcaacaaac tgttggcggc ttggacattc atactttccg caggatgaat
    50401 caggatcacg cgcaaaccgc cccgtaccga agtcaggtac acattatcga tgatttccct
    50461 gacggcatcg attttgatcc gcaacagttt gcgtccgttt tcgggttcgt ccgccagagg
    50521 cgtgatttcg taaaaatcgg gatggcttcc ccgtccaaac agatggcagg acatacattc
    50581 gccacagggt ttgcagcccg gtgcaggggt ttcgcacaac agcgccttcg ccgcaaagcg
    50641 ggcaaacgca gtcttccccg tccccttttt gccaacaaac agccacgcat taggacggct
    50701 cgtccaatgc tcggcaatct gccgccattg ttcctgatgc cacggataaa tcatgtcaaa
    50761 ctgcccgtcc ggaaatccat accgccatca gagcagttcc aaatcggggt cggaactttg
    50821 tgccctgcgg ctgcgcaact gtaccgccaa acgcaaatca tgtgcggaat cggcattttt
    50881 caaggcatcc tgcaaggaaa tctcgccttt ttcatacaat tggtaaaggt gttggtcgaa
    50941 ggtctgcata cccagggtag tggatttttt catcacttct ttgatttcat ggatgttgcc
    51001 gttgtgaatc aactccgaaa tcaggggcga attgagcagc acctcgactg ccgccaccct
    51061 gcccttgccg ccgtctcgcg gaacgaggcg ttgcgaaata aacgcctgaa ggttgagcga
    51121 caaatccgtc agcaattgtt cgcgccgctc ctcggggaag aagttgatga tgcggtcgag
    51181 cgcctgattg gtgctgttgg cgtgcagcgt cgccatacac aaatgccccg tttcggcaaa
    51241 ggcgatggcg tagtccattg tttcacggtc gcggatttcg ccgataagga tcacatccgg
    51301 cgcctgacgc agcgtatttt tcaacgccgc catccagttt tccgtgtcca cgccgacctc
    51361 gcgctgggta atgatgcagt ttttgtgttc gtggacaaac tcgatcggat cttcgatggt
    51421 gatgatgtgt ccgaacgaat tttcattgcg gtagtcgata agcgaggcga gcgaagtcga
    51481 tttgcccgag ccggtgccgc cgacaaaaat aaccagcccg cgttttttca gcgcaacatc
    51541 cttcaaggcc ggcggcaggt tcaggctttc aaacttggga atcttgctgg taatcgcgcg
    51601 gaataccaac gccgtcgcac cgcgctgtat catcgcattg acgcggaagc ggctggtgtc
    51661 cggcaggctg atggcgaagt tgcactcgtt ggtcgatgaa aattcttccg cctgcttcgc
    51721 actcataatc gaaaaggcga tttccataca tttttccgcc gtcagcggtt cgtccgtgat
    51781 gcgggtgatt ttgccgtcca gcttcatagc gggcgggaaa tgggtcgtca caaacaggtc
    51841 ggaacctttg tttttgttca tatggcgcag ccatgcgaac agttcttcct ttgcagtcat
    51901 attttcatcg gtaaacatcc tcgtccccca atcttcaaac aatctgtacc ttcccattct
    51961 accaaaaatc cgccgtgctg tctgaaaccg tttcagacgg cacggacaaa ccgcctccac
    52021 tcaatcctgc cgtgttagcg tgtataattc ggcgttctat ccgaaattca cagcaggtta
    52081 gcaatatgtc tttgaaaaca gtagccgtta tcggcgcgat ggaacaggaa atcgagcttt
    52141 tgcgcgagat gatggaaaat gtcaaagccg tctctttcgg cagattttcc gcctatgaag
    52201 gcgaattggc gggaaaacgc atggtgcttg cattgagcgg catcggcaag gtcaacgcgg
    52261 cggttgcaac ggcttggctt atccgtcaat tcgcaccgga ctgcgtcatc aacaccggca
    52321 gcgcaggcgg tttgggcaag ggtttgaaag tcggcgacgt ggtgattggc acggaaaccg
    52381 agcaccacga tgtcgatgta accgcattcg gctatgcccg ggggcaagtg ccgcaactgc
    52441 cggcaaggtt cgcttcagac ggcatattga ttgaaacggc aaaacgggcg gcgcggacgt
    52501 ttgaaggcgc ggaggtagaa caaggcctga ttgtcagcgg cgaccgcttt gtccacagca
    52561 gcgaaggcgt ggcggaaatc cgcaagcact tccccgaagt caaagcagtg gaaatggaag
    52621 cggcggcgat tgcccaaacc tgtcatcagt tggaaacgcc gttcgtcatc atccgcgctg
    52681 tttccgattc ggcggacgaa aaggcagaca tcagctttga agagtttttg aaaacggcgg
    52741 cggcaagttc tgcaaaaatg gtggcagaaa tcgtcaaatc tttataaaac ctttatcaaa
    52801 tcatgccaaa acagcggttt tccgttagaa tatcgggcta tttccacgtt gcgcccgata
    52861 ttcggacgta ccgctgtttg ttcagacggc atttctaaaa cctaccgttc agcattaaaa
    52921 acaactcaat gaaaaatatc cgaaatttct ccatcatcgc ccacatcgac cacggcaaat
    52981 cgacgcttgc cgaccgcttc atccaatatt gcggcggttt ggatttgcgc gaaatgagta
    53041 cgcaggtgct cgattccatg gacatcgaaa aagagcgcgg catcaccatc aaagcgcaaa
    53101 ccgccgcact caactacaaa gcacgcgacg ggcaggtgta tcagcttaat ttgattgaca
    53161 cgccgggaca cgtcgacttc tcttacgaag tttcccgttc gctgtcggct tgcgaaggcg
    53221 cgcttttggt cgttgacgcg tcgcagggcg tggaagcgca aaccgtggcg aactgctata
    53281 ccgcgattga tttgggcgtg gaagtcgtgc ccgttttgaa caaaatcgac ctgcccgccg
    53341 ccgaccccga gcgtgtggaa caggaaatcg aagacatcat cggcatcgat gccgtcggcg
    53401 cggtgcagtg ttcagccaaa agcggcatcg gcgtggaaga cgttttggaa gaaatcgttg
    53461 ccaaaattcc tgcgccgacc ggcgacgaaa acgcgccgct gcaagccgtt atcgtcgatt
    53521 cgtggtttga caattacgtc ggcgtggtca tgctgattcg agtgaaaaac ggcaccatca
    53581 aactgaaaga caaagtgcgc tttatgagca ccaaggcgga aacgcaggtc gagcagctgg
    53641 gcgtattcac accgaaatcg gttcaaaaac aagaactaaa agccggcgaa gtgggttttt
    53701 tgattaccgg cgtgaaagaa ttgggacagg cgaaagtcgg cgatacggtt actttggttg
    53761 ccaaccctgc cactgagccg ctgcccggtt tccaagaagt acaaagccaa gtatttgcgg
    53821 gtctctatcc tgtggaaagc cacgattacg aagccttgcg cgacgctttg gaaaaattgc
    53881 agctgaacga tgcttcgttg aaattcgagc ctgaagtttc tcaagcattg ggcttcggct
    53941 tccgctgcgg cttcttaggc ctgctgcatt tagaaatcgt gcaggaacgc ttggaacgcg
    54001 agttcgacat ggatttgatt accaccgcgc cgacggtggt ttacgaagtc gtgttgaaaa
    54061 gcggcgaaaa aatcgaagtt gaaaacccgt ccaaactgcc cgacatcggc agcatcgaaa
    54121 ccattctcga gccgattatt accgcgacca tccttgtgcc gcaggaatac gtcggcaacg
    54181 tcatgacttt gtgtaaccaa aagcgcggcg tgcaggtcaa tatgcagtat atgggccgcc
    54241 aagtgatgct gacttacgac ttgccgatga acgaagtcgt aatggacttt ttcgacaaac
    54301 tcaaatccac ttcgcgcggt tatgcttcgt tggactacca tttcaaagaa ttccaaccgt
    54361 ctgatttgat taagctcgac attatggtta acggcgaaaa agtcgatgcc ttaagcctga
    54421 ttgtacaccg tcaaagcgcg gttcacaaag gccgcgagct ggcatcgaaa atgcgcgaac
    54481 tgattccgcg ccaaatgttc gacatcgccg tccaagccgc catcggcagc cggattatcg
    54541 cccgagaaaa cgttaaagcc ctgcgtaaaa acgttttggc gaaatgttac ggcggcgata
    54601 ttacgcgtaa gaaaaaactg cttgaaaaac aaaaagcagg caaacgccgt atgaaacaag
    54661 tgggcaacgt ggaaattccg caaagcgcgt tcttggcgat tttgcaggtg agcgacaaat
    54721 aatatttcgg cacatagggc gggcgcgtcg ggaacacctc tcaaaccgcc ctgatttcag
    54781 cggcacgcgc ccgcaaacaa attttgcaat ttgccgcctt aaaacggttg cacggccggg
    54841 cgggttttta accgttaagg aacaacaatg aacacaatgc taatgtcggg cgcggctgcc
    54901 gcgctgcttg ccggcatcat cctttatttc aaaagcgaca agaaacggca ggaaaacggt
    54961 gaatggagtt ccggcctcga atacgcctat atcctgacgg cggtcggtgt gtttgccgct
    55021 ttgtccctgt ttatgagctt taccgccgtt ttcctgattt tcgttgtatt gtgcggtacg
    55081 gcttgggggg tatataaata ccgcctgaag actcatcccg aaatctcgga aagcagccac
    55141 ttcggcgatt atttcggcag tttcttccct accgttttgg tattgttcct catccggtcg
    55201 tttattgccg aaccgttcca aatcccgtcc agctcgatgc gcccgggatt aatcaagggc
    55261 gatttcattt tggtcggcaa attttcctac ggcctgcgcg tacccgtttt aaacaatgta
    55321 ttcatcccca cgggcaaaat cgaacggggc gatgtcgttg tttttaacta tcctttgcag
    55381 ccggagatga cctacatcaa gcgtattgtc ggcatcccgg gcgacattgt cgaatatcga
    55441 gataaagttt tgacggtaaa cggaaaaccg gcttccgaca ttcctgacgg cacataccgt
    55501 tatcctgacg ataccgaccc atccgaaatc cacaacacgg atatgttccg cagcggtctg
    55561 gacggcaaat ccttcaatat tctgaaaaaa gaaggacagc ctgccgtttc cctgcccgta
    55621 ttgggcaaat atacctccga tattatgtct gaaaacggat attccataga tcaaagcggt
    55681 ctgaaccatt gccaatatgc cgacgacggc agcggtttcg tgtgcaaagt tcccgaagga
    55741 cgctatttcg ctatgggcga caaccgcgac aacagtgccg attcgcgcta ctggggcttt
    55801 gtggatgaca agctggttgt cggcaaggca atgttcattt tgatgaactt cggcgatttc
    55861 ggcagggccg gcacggcaat ccgttaagcc ggaaaattac gccggaacat caaaaatgcc
    55921 gtccgaaccc tgtttcagac ggcattttta tcatcaaact ccttgtttcc ctaccggcag
    55981 cctcggggat aatatagtgg attaaatcta aaccagtaca gcctggcctc gccttgccgt
    56041 actatttgta ctgtccgcgg cttcgtcgcc ttggcctgat ttgaatttaa tccactatat
    56101 gcaccgcctt gccggcatta tcgatatggg cggcacatat ggattgcctg gaaaaatgat
    56161 gcgttcgggc ggcgtatgat ttgcctgtgt ttaaatcatc ggttgatttt atccgtcggc
    56221 aagtgaaaag ccggttattt ccgtttgtgt ggaaacccat cccattgaat aaagggcatt
    56281 tcgtccgact gacggtgtgg gcggcatatg gaaatacgga cattatttta gttgcattgc
    56341 cattcctgcg gaggcggctt ttttgattct gcccgccccg ataccgtctg aaagggcttc
    56401 agacggcatt ttccgctccg cgccgttgtc ggcatgtttg gtcttgccgg ttttgactgc
    56461 ctgccagtcc aatcgtacgc aggtttgcca cggcagaaaa tctgatgccg accggagtca
    56521 aagctcggac ggctgtttca gcgaaccgag gtaaatcgcc gaaagcgtca gcaccacccc
    56581 tgcccactgc acgccttcaa tcgttttgcc caatccgaaa taatcgatga acagggcggc
    56641 gaccggttcg gacaaaagca acagccccgt cagcgacagg gaaagcagcg gaatcgcata
    56701 ggcaaccatc gcccacgcaa aacactgcat caccacgccc cgtacacaag caccaaaccc
    56761 gaatctttcc aagtggtcgg ataaagtgcg ccgccgtcca tcagcaatgc cggaacaatc
    56821 ggcgatgccg cgccgcccaa actcaaaatc atcattgaag ggaaaagcgc cacctgttcg
    56881 acttcatgag tcttgcggac gaacaccata gatagggcaa gcatcagccc ggacaccaaa
    56941 ccgctggcga atccccaaac cgcattaccg ttgtagccga attccgcacc ggcaatcatc
    57001 gccacgccca caaccgccga catcaagctt gccgcctgca gcctgcccaa acgctcgccg
    57061 aaaaagaaaa caccgattgc cgacaagaaa aagatttgca ggctgttgag cagggtggaa
    57121 atacccggcc cgaccgcgtg tatgctttcg tgccaaagtg ccaaatcgaa agcgagaaac
    57181 acgcccgccg tcagggcata tcggacggtt tttctgtttt ttgggaattt ttgtccgaaa
    57241 aaccgtgcca aaaaccaaaa taccaatacc gaaatcagca accgccaaaa tgcgattgca
    57301 tacgggccga cggggacgga tctgacaatc aggctgccca atccgaacac cgcgcaaccc
    57361 agcaccagca atggtgcggc atattttttt ccgttaccga ccacaatatc ccctcccttc
    57421 cccatatggc tgccgcgccg ccggcaaaag tgccgccatt ccgaaaatat gccgtctgaa
    57481 gcccaaagcc ccggctgcgc ccggacaaac ggctgacttt acgggcaggc cggctatttt
    57541 ccgttggggc aaaatccgtc ctgccggata aaacccgaat aaaaaatatt tccctcaagc
    57601 aatgcggcgg cgccaacacc tgattaaaac acaaccggcg ttttcggtat atatgcaaaa
    57661 acgcattttc cggcacggcg ttacaccgat accgaaaaat gcgtctgaat gtttcgacaa
    57721 tccgattaaa gcattatgcc aaatctgcaa aaagtggcgt agagagatag cgttcgccat
    57781 aagaaggcag cagcacgact atcagcttgc cttcgttttc aggctgtttg gcaagctgca
    57841 acgcgctcca aaccgccgca ccggaagaaa tgcccgccaa aatgccttct ttttccgcca
    57901 ttgcacgggc ggtttcaaaa gccgcttcgt tcggcacttt ggcaatgctg tcgtagattt
    57961 tggtattcaa aacggtcgga ataaaacctg cgccgatacc ttgaatcggg tgcggacctt
    58021 tttcgccgcc gctcaatacg ggggaagcct caggctcgac ggcaaccact ttaacttcgg
    58081 gtttgtattt tttcaatact tcgcccacgc cggtaatcgt accgcccgtg ccgacgccgg
    58141 caacgaagac atcgacttta ccgtcggtat cgttccaaat ttcctcggcg gttgttttgc
    58201 ggtggacttc gggatttgcc tcattgtcga actggcgcgg cataaaataa gtgtctggat
    58261 gagcgtccac caaggattgc gctttggcaa tcgcgcccgc cataccttcg gcggcggggg
    58321 ttagaatcag ttccgcgcca aacgtgcgca acagcatttt gcgctctttg ctcatgcttt
    58381 ccggcatggt aatcgccagt ttgtagccgc gtgcggcaca taccattgcc aaaccgatac
    58441 ccgtattgcc gctggttgct tcgacaatga cggtgttttt gttgattttt cccgcttttt
    58501 cggcggcctc gatcattgct tcggcaatgc ggtctttgac gctgctgccc ggattaaaaa
    58561 attccagttt cacggcaacc tctgccttca aacctttggt caaacggttc agtttgacca
    58621 aaggcgtgtt gccgatcaat tcggtgatgc tgtttgcaat tttcatcttc tgctcctgaa
    58681 taataaggtt tcgacagtaa ggcgcaatcc ccatcggtat cctgtccgat tgcaggggat
    58741 ttgcgccgaa ccataaacaa atttcggata tggctttatc ctaaacctat gccgcctaaa
    58801 aaaccaatac ccaaaattta tttctttata actttttgtt ccgatacaga cggcatcaca
    58861 atcatctgcc gatttcggca agcagcatcg ccgttaccgc aacctgcatc aacatcgccg
    58921 ccaacacgcc gagtcgcagc tgcgaaccgc cgaacgttga cggcggctgc cggtctatac
    58981 cgcagcggtg gacataggca accgccatca atacaaactg caacacgaac caataaaccg
    59041 gcttcagcgc ataaagtttg agcaatgccg caaacacaaa cacttgaacc gttcccgaaa
    59101 agcaataatg caccaacgca catattccga caaaagcaag cgtcatcgat acattactga
    59161 cggcaaaaag cccgagcagc ggatgttcgg ggtctgcctg ccatccgttt ccatctgttt
    59221 tccggttcca atacccgatg aaaaaaaata tgctgcccaa agtcaggtaa aaatggggga
    59281 tgaacaaagg cgcggcgcgg gtcattcccc acatccccgg catcagcttt acccctaaaa
    59341 ccgagatgcc gagccacaac gcaatactcg cccaaaacca ttgcaacatt cctgcgcgga
    59401 atatcagaaa cagaaaaacc gccgcataca gtaaggcaga caaaaaatcc aaatgcagca
    59461 aaatcatgaa tacgccaact cgccttcaaa cacggtttcc gcagggccgg tcatcatcac
    59521 atcgccgccg caggcccatt cgatatataa agtcccgccc ggcaaaacca cctctaccgt
    59581 tttcccttca tccaacagcc ccagacggat acccgccacc acagccgcac acgcgcccgt
    59641 accgcacgct tgggtttcgc ccacgccgcg ctcgaacacg cgcaaacgga ttgccgtccg
    59701 tccgacaacc tgcataaagc cgacgttgac gcgttcggga aactgcctgt gcggttcgat
    59761 acgcgaaccg gtttcgcgca ccggcgcgca ttccacgtca tcgactacaa tcacagcatg
    59821 aggattgccc atattgacgc agctgacagg ctgaatgccg gattcgagat gcaccccgta
    59881 aatacaggca tcatccccct cgcccgattc ggggacaaac ggtatttcag acggcataaa
    59941 cttcggtttg cccatattga ccgtaaccat accgttatcg gacaatttcg gaaaaataat
    60001 gcccttggcc gtttcaacac aaatttcttt cttatcggtc aaacccttgt ctgcaacaaa
    60061 acgggcaaaa caacgcgctc cgttgccgca ttgcccgacc tcgctaccgt cggcattgaa
    60121 aatacggtaa cggaaatcca cgccttcggt ttccgaacac ccgaccacca aaagctggtc
    60181 gaagcccacg ccccggaagc ggtccgccca tgccgcaatc ggcacgtcct cgggggtaaa
    60241 atcctgactg accgcgtcaa tcaccataaa atcgttgccc aaaccgtgca ttttggtaaa
    60301 tttcaaagtt ttcatattgt tccgaaatcc cgtattgtct gttgcttccg aatccgaaat
    60361 ccccgagccc gaagggaaat gcttccatct cccgccgcct gaggattaaa atgagccatt
    60421 atactgtttg ttacccctta caagaaacac aatgtcccgc ctgttcctgc tgcttttccc
    60481 ttgggcaacc cttgccgcct gcacccgaaa cataccggca agccccgccg tttcaaattg
    60541 cgcgcatctt cacaaccgca ccgtcctgta ttttgacgca cacggcaaac ccgccgacag
    60601 cacagcaaag ggcggatttt acaggaccgt cgaaattcat gacacaaacc gcatccttat
    60661 tcaagacttt aacagcacgg ggaatgccct cgtcaaaccc tattggtcgg actgccggaa
    60721 attgtccgtt ttccccgaca aacatctgac catgagccat ccatgaacca cgacatcact
    60781 ttcctcaccc tgttcctgct cggtttcttc ggcggaactc actgcatcgg tatgtgcggc
    60841 ggattaagca gcgcgtttgc gctccaactc cccccgcata tcaaccgctt ttggctgatt
    60901 ctgctgctta acacaggacg gataagcagc tatacggcaa tcggcctgat gctcggatta
    60961 atcggacaac tcggcatttc actcgaccaa acccgcgtcc tgcaaaatat tttatacaca
    61021 gcctccaacc tcctgctgct ctttttaggc ttatacttga gcggtatttc ttccttggcg
    61081 gcaaaaatcg agaaaatcgg caaaccgata tggcgcaacc tgaacccgat actcaaccgg
    61141 ctgctgccca taaaatccat acccgcctgc cttgctgtcg gaatattatg gggctggctg
    61201 ccgtgcggac tggtttacag cgcatcactt tacgcgctgg gaagcggtag tgcgacaacc
    61261 ggcggactgt atatgcttgc ctttgcactg ggtacgctgc ccaatctttt ggcaatcggc
    61321 attttttccc tgcaactgaa aaaaatcatg caaaaccgat atatccgcct gtgtacagga
    61381 ttatccgtat cattatgggc attatggaag cttgccgtcc tgtggctgta agccccattc
    61441 attcccgata cccgtaaaaa ataccgtctg aaacctgttc agacggtatt ttcaccctaa
    61501 gcgttactcg gcggcatttt cttcacggac aggcgcatcc agcagggctt tgatggacag
    61561 acggacacga cctctgtcat ccacttccaa tgctttcacg ttcaccacct gaccgacttg
    61621 caggtaatcg ccgacattgc gtacgcgctc gtgtgcgatt tggctgatgt gtaccaaacc
    61681 gtctttgccc ggcatcacgc tgacaatcgc gccgacgttg ttgtcgagga ttttaaccac
    61741 agtaccttcg tacactttgc ccacttccac ttcggcagtg atttcttcga tgcgtttttt
    61801 cgctgcatcg cccgcttctt gagtagtcgc agcgatagtg atggtaccgt cttcagcgat
    61861 attgatttca gtaccggttt cagcggtaat cgaacgaatg gtttcgccgc ctttaccaat
    61921 cacatcacgg attttgtctt ggctgatttt catggtgaac aaacgcggcg cgtgtgcgga
    61981 cagctcttgc ggacccgcaa cggcagcttt catctgatcc aagatgtgca aacgcgcttc
    62041 tttggcctgt gccaaagcaa tttgcataat ctctttggta atgccttgga ttttgatgtc
    62101 catttgcagc gcggtaacgc cttcggtcgt accggccact ttaaagtcca tatcgcccaa
    62161 gtggtcttcg tcgcccaaaa tgtcggtcag gacggcaaac ttattgcctt ccaaaatcag
    62221 acccatcgcg ataccggcaa cgtgtgcttt caaaggcacg ccggcagaca gcaggctcaa
    62281 acagccgccg cagacagaag ccatagagga agagccgttg gattcggtaa tttcggagac
    62341 cacgcgcatg gtgtagctga aatcttcagg tttcggcaat acggccaaca acgcacgttt
    62401 agctaaacgg ccgtgaccga tttcacggcg tttcggcgcg cccatgcggc ccacttcgcc
    62461 ggtagagtac ggcggaaagt tgtagtgcag cataaagcgg tcggtatatt cgccggacaa
    62521 cgcgtcgatg atttgttcgt cgcgcgaagt acccaaagtc gctacggcaa gggcttgggt
    62581 ttcgccacgg gtaaacagtg cggaaccgtg cgtgcgcggc aatacgccgg tttggatgtt
    62641 cagcggacgg acggtgcggg tgtcgcggcc gtcgatgcgc ggttgtccgt ccaaaatttg
    62701 gctgcggacg acatcggctt ccaagtgttt gaaaatacct ttgatttcgt tggctgccag
    62761 cgtgtcggtt tcttcggtaa tcaaggcttc ttttaccgca ttccaagcct cgtccaattt
    62821 ggcggaacgc gcttgttttt gacggatttt gaacgcttct ttaatggttt cgccggcaat
    62881 cccgcgtact ttggcaacca gttcttcatt ggtttcaggt gctttccaat cccaaacttc
    62941 cggattcact tcgtcggcaa attcattgat tgcattgatg gcaacctgca tttgatcgtg
    63001 gccgtaaacc accgcgccca acatcacgtc ttcgggcagg attttggctt cggattccac
    63061 catcaacacg gctttggaag taccggcgac caccaagtcc aattgtgatt tcgccaactc
    63121 ggctttagtc gggttcaaaa cgtacacgcc gtttatataa cccacgcgcg ccgcgccaat
    63181 cgggccggcg aacggtacgc cgctcaacac cagcgcggcg gacgcgccca acattgcagg
    63241 aatgtcggaa tcgatttcgg gatcgacgga cacaaccatc gctacgattt ggatgtcgtg
    63301 gtagaaacct tcggggaaca gcggacgaat cgggcggtcg atcagacggc tggtcaggat
    63361 ttctttttcg ctttgtttgc cttcgcgttt gaagaagccg ccggggattt tgcctgcggc
    63421 ataagtgcgt tccaaataat cgacggccaa cgggaagaag tcctgacctt ctttgacctc
    63481 tttgttggtg gtaacggcaa caaaaacaac agtgtcgccc atcgatactt taacggcagc
    63541 ggcagcttgg cgggcaattt cgccggtttc caaagtaacg gtttgattac cgtattggaa
    63601 ggtcttaacg tatttattga acatcattgt tcctttcaaa ataccgcact gctaaaacac
    63661 taataatgca cactaaaatc cgaatgtgca tagttagggt ttcagaccgt gcggcaggtt
    63721 gtaaacaagc tttcagacgg catttcggac gctgaaaaca attgcggatt ataaaggcaa
    63781 ccatccttaa aatccagtat tgatacaata aaaaggccgt ctgaaacata tccttttcag
    63841 acggccttca gtcttcaaaa tcaatcttgt gtcagtaaag tcaatgcttc ctgatatttc
    63901 tcgaccgttt tccgaattac atcggcaggc actttaggcg caggggcttt tttgttccaa
    63961 ccgctttgtt ccagccagtc gcggacgaat tgtttgtcaa aagacggcgg attggtgccg
    64021 acttcatatt gatccgccgg ccagaagcgg ctcgaatcgg gagtcaatac ctcatccatc
    64081 agcgtcagcg tacctttttc atccaaaccg aattcaaatt tggtatcgca aatgatgatg
    64141 ccgcgcgatt tggcatattc cgccgcttcg gtgtaaagcc gaaccgcttt gtcgcgcact
    64201 tcttccgcca attctttacc gataatgcgt ccgcattctt caaagctgat gttttcatcg
    64261 tgatcgccga ctgcggcttt agttgagggc gtaaaaatca cttcgggtag ttgttgcgct
    64321 tcccgcatac cttcgggcag ccgaataccg caaaccgagc cggttttttg ataatctttc
    64381 caaccgctgc ctgccagata accgcgcaca atggcctcca ctttcaccgg agtgagtttt
    64441 ttagctacaa cggcgcgttt ttccaaagct ttggcttcgt tttcaggcaa aacatcgtac
    64501 acggtatcgc cggtgaaatg gttgggcata atatgcgcca gttttttaaa ccaaaaattg
    64561 gaaatctgcg tcaggatttc gcccttaccc ggaatcgggg cgtccaaaat cacatcaaac
    64621 gcggacaggc ggtcggaagc gaccatcagc atacgtttat cgtcgatttc atataaatcg
    64681 cgcacttttc cggaataaat ctttaccaaa ccaatctcac tcatttcgca cccccctgaa
    64741 aatatcttga aaataccgac ccgacaggtt tgaatcgcaa accgatattc tagccgaagt
    64801 cggcgcaaaa caatacccat ggcacaaaaa gccaacccgt caaccgtcgg caaagttttg
    64861 gcactataat accgacagca agtcctacaa tacactttca ccaaaggaaa tacctcatga
    64921 gaatcctatt gacaggctcg aaaagccaac tggcacactg cctgcgcgac cgtcttcctg
    64981 aagactggga aaccattgcg acggattccg catccctaga cattaccgat gccgatgccg
    65041 tctgcaacat ggtcaaaagt ttccaacccg acgctattgt caacacggct gcctatactg
    65101 ccgtcgacaa agcggaaggc gatgcggcag cggcatttgc cgtcaatgct tccgccgttt
    65161 acaaccttgc cttggcagca catcgcgccc atgcccgatt catccacatc tcaaccgact
    65221 atgtctttga cggaaaaggg aaaataccct atcaggaaag cgactttacc aacccttcca
    65281 atgtatacgg acaatccaaa accgccggcg aactgctcgc actgtctgcc aatcccgaca
    65341 gccttatcct gcggacttct tggctgttta gcgaatacgg ggacaacttt gtccgcacga
    65401 tgctgaacct tgcgcgggaa cgttccccgc tgtccgccgt ccacaaccaa atcggctgcc
    65461 cgacctatgc cggcgacttg tccgccgcca tcatccgcct gttgcagcag tccaatcccg
    65521 ttcgcggcat ttaccactac gccggcggca aatccgtatc ctggtacgaa tttgcccggc
    65581 acatctttca gacggcattg caacaggatg cctcttttcc cgttcccgaa ctgaaagccg
    65641 tttcggatga aggctctgcg gctgccgccc ccaggcccgc atacagcatt ttggactgcc
    65701 gcaaaatcga aaacgacttc ggcatcaaac cgtcagactg gcaaaaagcc cttgcacagg
    65761 tcgtttccaa gctgctctga tgccgcccgc ccctccgttt cctccgccaa gcaccgcctt
    65821 ggcggttttc ttataaaatc tactgattac acaaacacat atcatcttta cacaatcatg
    65881 cttccatcaa cagcaaaaca tgatatgatt gccaacaatg acatctgaca ataaattttc
    65941 taatttttat tgaaaaaatc aataaattaa gaattctccc aaccgacaaa taaaataaag
    66001 aaagggtcaa tatgcaacac cgtagaagat tggcaattta ccaggcatcc aaacgtgctt
    66061 cctttaccgg caggtcatca gccccacaaa aacgtaaaga acgttgattt gaaaaaaatg
    66121 ccgtctgaag tcctgcttca gacggcattt tttaccgttc gagaaactgt ttcaacctgt
    66181 cctcatccaa aaaccagtgg caaatttcct catctcccgc caacaaaacg ggaatcagct
    66241 cattgtattt ttcttccaaa acaggatttt catccacatc gaccacttcc agcccgaacc
    66301 cgtattcatc ctgaaaaggt ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt
    66361 cacgaaacat caaggtcaat ttcatccgcg tttccttatc tgtcaatttg cacacgccaa
    66421 agccttagac gcagcagaat catggtctat ttgggaaaaa acaatgtctt cgaggaagat
    66481 gatactcaag tcctgccaaa aacagcaaaa atgccgtctg aacagttcag acggcatttc
    66541 caaaaccgtt ttacgcttga acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg
    66601 ccgtcagaaa cgaagcggca agctccccgc agcagccgca gcagctcgcc acgcccaatt
    66661 gcgactgcaa atcgcccatt gtggtcgcgc cggcagcgat ggtttccttg atttgatggt
    66721 cggtaacggc attgcagatg cagacaaaca ttttgtgctc cgtgtgttct caaactatcg
    66781 taccgatagc ggcttttatt atcgtatgca aatataaata aaaacggttc gcattgcaag
    66841 gtcggcatac acggtttgtc ttggtaattt tttatcaagt ttgcattttc gattgatttc
    66901 ttattaaacc gaagcagaag cctcagtttc gggaatggtg ttccttaacc acgctatgca
    66961 gccgcacatt ggcaacatgg gtataaatct gagtcgtgtt caaatcggcg tgtcccaaca
    67021 tatcctgaac tacgcgcaaa tccaatccgt gccgcaccag atgcgtggca aaggcgtggc
    67081 gcaggctgtg cgggctgatg tgcccgatgc ctgccctgcc ggcatattct ttgacaatca
    67141 tccacgccaa ctgccgggaa atgcccgttt ttttctgact gacaaacaat gcgtcgcaac
    67201 tcctgccttt cagcagaagc gggcgtgcct ccgtataata gcgttccacc caatacgctg
    67261 actcctgtcc catcgggacc atcctctgct tgtcgccctt gcccagcgtg gtaatacaac
    67321 ccctgtccaa atccacattg ccgaaattca gcccgaccgc ctcgctgacg cgcaagccgg
    67381 tcgcgtacat caattcgagc agggccttat cccgcaaacc gtgcggcgtg tcggtatccg
    67441 gtgcggcaag cagtcgggaa atctgctgtt ctgtaatcag tgtcgggatg tttctgtcga
    67501 tttttggcgg tttcagcagg cgggtgggat tgtccgccct catcccttcc cgttccatcc
    67561 acacatacag gcgtttgcac gccgacagtg cgcgtgcctg cgaactccgt tgctccccgt
    67621 caacataaac cgccgccgcc aaatctgctt cgtccgcatc cttcagcgtt ctgccgcaca
    67681 aagacagccg gcgggcgatc ttttccaaat cccgccggta gctgtccaaa gtattccgac
    67741 tgagccgccg atccaaccac aaagtttcca gcagcctgtc aatcaaaccg ttttccataa
    67801 cgttccaaac caatgccgtc tgaatcttct tcagacggca tggttcacat catcgggaaa
    67861 gcgtttccaa cacttcctgt gcatgacccg ccactttgac tttccgccac tcccggacaa
    67921 tttcgccgtc cttgttcaga acgaacgtac tgcgctcgat gcctaacgac tctttcccat
    67981 acagtttttt caatttgatg acatcaaaca ggcggcacac cgtttcatcc ttgtcgctca
    68041 acagctcgaa ccggaaaccc tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc
    68101 gggaaatacc gaccacggta taacccaatg cctcaaactg ttccaaacgc gcattaaaat
    68161 ccaagccttc cgtcgtacag cccggcgtac tgtctttcgg ataaaaatac acgaccaaag
    68221 gcagatgttc tgccgaatga aaatccgcac cgctgctcga aggcagggta aattcatatt
    68281 tcatatccat agtcctactc ccgatattcc cattattcaa aacggcacgc agacgcaccg
    68341 ccgcaattgc caaaccaacc ccgattctac cgccccaaag gacaaggatt caaccgccgg
    68401 aaacatccaa accgacacac gacggcatga aaaatatcca tgtcaaacca caaaatatgt
    68461 tccgatttaa aaacagaatg ttataaaacc caactccaaa aacacaacaa gaccgcccgc
    68521 ctacgggcag tctcctgtca gacgacatac tttacagatg gctgtttttt caacaaaata
    68581 acgccaatac tcaaaaatac ggaaccaaaa atgtccatcc gcaccctgaa acgcctgccc
    68641 tcatcgctgc tgctcggcct ctgcctttcc ctgccgtcag cccacctttt tgccgacaac
    68701 gacattttag ggcaattttt agaacagaac atgcttacct cctccgatcc gatagaaata
    68761 ttcgccgaaa gcacgatata tcccgccgat acccaggcca ttacgggcgg gctgattctg
    68821 tcctcccaat ccgcactggt cgtcaacaac aaaacagggc aaatactgta tcagaaaaac
    68881 gccgacagga ttatgcccat tgcctccatt tccaagctca tgagcgcgat ggtcgttttg
    68941 gatgcaaact tggacatgaa cgaaaccgtt accatcacac ccgacgaaat cgaccgcctc
    69001 aaagggaccg gcagccgtct tgccataggt acggcactta cacgtaaaga actgctgcac
    69061 cttagcctga tgagcagcga aaaccgcgcc acccatgcat tgggcagaac ctaccccggc
    69121 ggcatgggcg catttgtcgc cgccatgaac cgcaaagccc aaagcctggg catgtacggc
    69181 agccgctttt acgaaccgac cggactcaac ttccaaaacg tttccaccgc caaagacctg
    69241 agcctcatgg tcaacgccgc cgcccaatat ccgcaaatcc gcaccaactc gacttccaac
    69301 tacgcctcgg tacaaaccaa aaacgggcag caaaactata aaaactccaa tgccctggtc
    69361 agggaaggca tgtggaacat cgaattgcag aaaaccggct acatccgcga ggcaggcagg
    69421 tctatggttg tcaaagccaa cattcaaaac caacccgtta ccatcgtatt gctgaactcg
    69481 cccacatccg ccacacgcgt caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa
    69541 cgctcctgac atacaaatgc ccggcggaaa accgcccaaa cacaaaaaaa ttggtaattt
    69601 ttcctattcc aggctacaat tcgacacgca cagggcatct gcccgatttc acaatcataa
    69661 tagcggagtt aaaaaatatg acagctaaag gacaaatgtt gcaagatccc ttcctgaacg
    69721 cattgcgtaa agagcatgtt ccggtttcga tttacttagt taacggtatc aaattgcaag
    69781 gtcaggtcga atctttcgac caatacgtcg ttcttctgag aaacacttcc gtaacccaaa
    69841 tggtttacaa acacgccatt tccaccatcg taccggctcg ctccgtcaac ctccaacatg
    69901 aaaacaaacc ccaagccgca ccggcttcga cccttgtcca agtggaaacc gtccagcagc
    69961 ctgccgaata atccgcacga agcatgacgt gtcatatctt tcaatacctt accggacaac
    70021 ggtaaggtat ttttattttc aggcggcatt taaaaatatt attgaaaaac atccttccat
    70081 tcgcccattg ccttttgcgg gaggcacttc ccgaaggcgg caatgcgctg gacggcaccg
    70141 ccggcaacgg acacgacacc cttttcctcg cacaaaccgc aggcaacagg gggaaagtgt
    70201 gggcattcga catccagccg caagccctga acaacacccg atgccgtctg caggaagcag
    70261 gttacagcaa tgtacggctc atcttggacg gacatgaaaa cctgaagcaa tatattccgg
    70321 agccgctgga tgcagccgtt ttcaatttcg gctggttgcc cggaggcgac aagaatctga
    70381 ccaccgacaa ggaaaccagc attcccgccc tttgagcaac cctctccctg ctgaaagaag
    70441 acggtatgct tattgccgtc ctctatccgg gacacgaaaa cggcaaacag gaggcagaag
    70501 ccgtcggaca atgggcaaaa aacctgcctc aagaacagtt tgccgtttta cgttacggct
    70561 ttaccaaccg gaagaatagc ccaccctatc ttttggcatt tgaaaaactg cgtcaaaaat
    70621 aactgtttgc ggtaaaataa gccgattcta acccttattt tgatacgagc cgaccatgca
    70681 gtatctgatt gtcaaataca gccatcaaat cttcgttacc atcaccattt tggtattcaa
    70741 catccgtttt ttcctacttt ggaaaaatcc agaaaagccc ttggtcggct tttggaaagc
    70801 actgccccac ctcaacgaca cgatgctgct gtttacggga ttgtggctga tgaagattac
    70861 ccatttctcc ccgttcaacg cgccttggct cggcacaaaa atcctgctcc tgttcgccta
    70921 catcgcactg ggcatggtaa tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt
    70981 ttacctgctc gctatgtgtt gcatcgcctg catcgtttac cttgccaaaa ccaaagtcct
    71041 gccattctga aacactgcca tgaacaacag acattttgcc gtcatcgcct tgggcagcaa
    71101 ccttgacaac cccgcacaac aaatacgcgg cgcattagac gcgctctcgt cccatcctga
    71161 catccggctt gaacaggctt cctcactgta tatgaccgca cccgtcggtt acgacaatca
    71221 gcccgatttc atcaatgccg tctgcaccgt ttccaccacc ttggacggca ttgccctgct
    71281 tgccgaactc aaccgtatcg aagccgattt cggacgcgaa cgcagtttcc gcaatgcacc
    71341 gcgcacattg gatttggaca ttatcgactt tgacggcatc tccagcgacg acccccgcct
    71401 taccctgccg catccgcgcg cgcacgaacg cagtttcgtc atacgccctt tggcagaaat
    71461 cctccctgat tttattttgg gaaaatacgg aaaggttgtc gaattgtcaa aacggctggg
    71521 caatcaaggc atccgtcttt taccggacag gtaattccgc acgcggatgc cgtccgaaca
    71581 ccggtttcag acggcatttt tcctttgccg ccaacacgcg tgcaaaaaaa atcgcccctt
    71641 gagaaagggg cgcaaaagga acacaaacca ctaccaaaac tttaaatctg caacactgcc
    71701 tgccgcatac tgtacccgac aggatataag gccctcacta aatcgtttcg agaaatccaa
    71761 acttcttcat caccgacaga aaatctgcct ttctccggca ccagctccaa caaaaacggc
    71821 tgaaccgccg tatgcagctt gtccttacac cgccccagct ttctggacaa cgcggacagg
    71881 gcgcgtttgt aggcattatc cttgcggtcg agctccctcg cactaccgac ccagctcaaa
    71941 cgaaggttgc ggctttcatc ttcaatcaac aaaatacctg tttcaacttc ccccacctcg
    72001 ggttcgcacg aaagcgcaat ataatatttg ccgtcaatat tgacatatgc cgcttcatgc
    72061 acctgacagt tgcaacccga ggttgcgagc agggaataaa cagaatcacg gtgcagcctc
    72121 aaaatatcgg aggaaatctg tttgaaaata ctttccggca tttttccctc ctttcgtctg
    72181 ctgcgttgtt tactggattg actgcattat acagacatct cccaatttta caaatgataa
    72241 ttgttcctat tttttaaaaa gtttataaat tattgataaa tatatgaatt aatttttaag
    72301 atttaaaacc tttgtctcaa aaaatacaaa aatgccgtct gaaacgtgtt cagacggcat
    72361 tggcttcaca ggcagtccgt ttaaaatttc acgcccttat gcagcgcgac gatgcccgca
    72421 ctcatattgt gataatccac gctgtcgaag cctgcatcca gcatcatctg tttcaaagtt
    72481 tcctgatcgg ggtgcatacg gatggattcg gcgagatact ggtagctgtc cgcatctttg
    72541 gcaatcagcc tgcccatcac cggcaacagc ttgaaagaat aaaaatcgta tgcgccttcg
    72601 agcggcttgt agattttgga aaactccaac accagcaacg taccgcccgg tttcaaaaca
    72661 cggtacattt ccttaagcgc ggcatcttta tacgtcatgt tccgcaagcc gaacgccacg
    72721 gagaccaagt tgaaataatt gtcggggaaa ggcagttttt ccgcatcggc aagcgacacg
    72781 ggtaaaatca tgccttcgtt caacagacgg tcgcgcccga cggtcagcat agaagaatta
    72841 atatcggtca gccaaacctc gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa
    72901 tcgcccgtac cgcccgcaat gtccaacact ttatcgcctt ttttcaggtg cgccgtatgg
    72961 atggtaaaat gcttccatac gcggtgcagc cctgccgaca tcacatcatt cataatgtcg
    73021 tagtttttgg cgacggagtg aaacacttcc gccacttttc cggctttctc atcttcgttg
    73081 accgtgctga acccgaaatg cgttttttgt ccgcccatat tctaaccacc tccctgatac
    73141 cgccctacgg catcccaaac ttaacaatct gattgctttt caaaacgcga cgcgcccgcc
    73201 gccgccaatt tgtccaaata ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc
    73261 agatacgccc aatcgtaaag accgctgtcg tgcccgtccg aaaaactgat tttcagcgca
    73321 tactgtccga caggctgcaa atccgtaatc tgcacatccg ccttaccggt ctgcaaaaca
    73381 tcctgtccca cgccgtgtcc gcgcacttcc gcactcggcg aatagacgcg taaaaattcg
    73441 gcaggcagat ttttaggttc gtccccataa gtcaaaacca acacggcacg gtttttcaac
    73501 aaacggattt caacaggaat attattcaaa aaacacctcg gttcggcata caatatccgg
    73561 caggcgtacg cgtgccttta aacggcgcgt attttaacag ctttatcccg ataccgatat
    73621 tcaaacccac acttcagacg gcattgatat ggacaatctc gtatgggacg gcattcccga
    73681 catccgcaca ctcgaccaaa ccatccgcaa acacgcacac ccgctcaacc tgattgtctg
    73741 cctccccgat aatcagattc ccgattttca aaccgcacaa gatgcttcgg actcggaatg
    73801 ccgtctgaag caccgtttgg atcaggcaac ccagtgcctc cagttcgaca gcatcaacct
    73861 catcgaacac atcctgcccg atgtccgctt ctggctggtt cccccttcac gcacccgccg
    73921 cctgcacgaa cacttccacc acatttcctg gcagaccgaa gccatcccgc aaaccgaaag
    73981 caagtccgac aaaccctggt ttgcacttcc acaaacatcc gaacggaaaa aaccggaaca
    74041 cgtcctcgtc atcggtgcag gcattgccgg cgcatcgacc gcccacgcct tagcatcaca
    74101 cggcatttcc gttaccgtat tggaagcccg aaaagccgct caagccgcca gcggcaaccg
    74161 gcaagggctg ctttacgcca aaatctcgcc gcacgacacc ggacagaccg aactgctgct
    74221 tgccggctac ggctacacca aacgcctgct cggacacatc ctgcccgact ccgacacttg
    74281 gggcggcaac ggcatcatcc acctcaatta cagccgcacc gaacaacaac gcaatcacga
    74341 attgggtttg caaaaacacc ataaccacct ctaccgcagc atcacgtctg cagaagccga
    74401 aaaaatcgcc ggcatcccgc tgaacacgcc ctacgccgaa ccattatgcg gactctactg
    74461 gcaacacggc gtatggctca atccgcccgc attcgtccgc accctcctca gccatccgct
    74521 gatcgaacta tatgaaaaca caacgttaac cggcatttcc cacgacggag aaaagtggat
    74581 tgcaagcacg ccaaacggca catttaccgc cacacacatc atctactgca ccggcgcgca
    74641 cagcccctgc ctgcccgaaa ccaacctcgc cgccctaccc ctcaggcaaa tacgcggaca
    74701 aaccggactc acaccgtcca ccccgttttc cgaacaactg cgttgcgccg tttcaggcga
    74761 aagctacatc agcccgtcgt ggcacggact gcactgctac ggcgcgagtt ttattcccaa
    74821 cagcagcaat accggatgga acgaagccga agaagcctca aaccgccaag cattggcaca
    74881 ccttaacccc gcccttgccg aatcattgtt tgccgccaac ccaaaccccc aaaaacacca
    74941 agggcacgcc gccatacgct gcgacagccc cgaccacctc cccgtcgtcg gcgcactcgg
    75001 cgacattgcc gccatgcggc agacttacgc caaacttgca ctggacaaaa actaccgcat
    75061 cgacaccaca tgcccatatc tgcccaatgc ctacaccaat accgcccacg gcacacgcgg
    75121 gcttgccacc gcccccatct gcgccgccgc cgttgccgcc gaaatcctag gcttgcccca
    75181 tctcttttca caacgcctgc gccacgccct acaccccaac cgcaccgtca tccgcgccat
    75241 cgtcagaagg cagaacctga ttccttaagc ctttgccgca tgccgtcccg accacaaaaa
    75301 atcatattca aataaacata ccaatcctga cggtaaaata tcgaaaacac ccataacgtt
    75361 ttcttcccaa aataaggagt aaggaatgcc taacaaattg cctttgctgt ccaagatgaa
    75421 ttttccgtaa cattcgaaga cttccttaac cctgccagcc gcttcggttt ctgtaccccc
    75481 tgttagataa ggaagggatg aaaaagccct gttacttgta tgaagcaaag ggcgttaatt
    75541 tttgttaatc ttcccttctc aggaactcaa tatataagga tgttaatttt tatttccctt
    75601 ctcgaggact caatagtatg agaaatgaga aatgagaaat gagaaatgag aaatgagaaa
    75661 tgagaaatga gaaatgagaa atgagaaatg agaaatgcct ccgatataat cggaggcatt
    75721 ttgaattggc acgcccacgg ggaatcgaac cccggttacc gccgtgaaag ggcgatgtcc
    75781 taaccgctag acgatgggcg catattgtat tttcttactg gcgcacccgg agcgattcga
    75841 acgcccgacc ctctggttcg tagccagata ctctatccaa ctgagctacg ggtgcggcac
    75901 aatcacttgt tcaagtgatt aagaaagcgt gataatacgg atttatctga gtcctgtcta
    75961 ttattttaga cttctatttt cagttatttt tgtaactgat tgattttaaa ataaactatt
    76021 tttcgacgca agttgcagca attctgccgc atgttgccgc gtcgtatcgg taataacttc
    76081 tccgcccaac atacgggcaa cctcttcgat ccgttgtatt tcatccaata tactgatttc
    76141 gctgacggtt tgctctccct cgctgtgctt gcgcacccgc cagtggtttt ctccgcagga
    76201 tgcgacttgc ggaagatggg taacggcaag cacctgatgt tttctgccca acgcacgtaa
    76261 tgccttgccg accatttcag ccactccccc tccaataccg gtatcgacct catcaaaaat
    76321 cagggtggga acttgggtat attggctggc aacaacctgt aaggcaaggc tgatacgcgc
    76381 caattcgccg ccggaggcga ctttgttcag cgggcggggc ggattgcctt tgttagcggc
    76441 aacttgaaat tgaacctgct ccaaaccgtg tgccgtcggc gacgaaggca acaggacgat
    76501 gtcgaaacgc gcgcctttca tggcaaggtg ttgcatatgc tcggtcgttt cgctactcaa
    76561 acgccctgcc gcctgatggc gcatggcaga aaggatgtgg gcagcttcct gatattcggc
    76621 aaaattgtgg gcaacattat gctcgagcgc gtccaaatca gcggcagctt gaagactttg
    76681 caggcgttct tcgatttctg ccaacttggc aggcaactct tcgggttcaa tccggtattt
    76741 ccgcgccatc cccatcagtt cgcccatacg ctgttcctgt gccgccaatt cattggggtt
    76801 gatgtcactg tgtcctgcca catcgcgcat attggcactg atttcgccca attcggcctc
    76861 gatgcttgcc aacatattca ggctctcggc aaagcgcggc tcgatgtttt gcagattggc
    76921 caatagtttt tgacattgat agatatggcg ttggatgccg ttgtcgccgt caatcttgct
    76981 tccgacttct tcggcggcct gcaacaattc ggcagaatgg gcaaggctgt cgtggctttg
    77041 gctgagagct tcccattcgc cttgtttaat gtccaactga ttcagttcgt taaactgcca
    77101 ttccagacgc tcccgctcga taatgacggc atcggcgtgt tcctgcgcct cttggagggc
    77161 ttttttcgca ttggcccagt tttgataaag ctgcctgacg gtttccgcct gcaccctgcc
    77221 gcccacaaat gcgtccaaca attcgcgctg ggcagcttcc tgattaagcg aatgatggtc
    77281 gttttgcccg tggatgtcga taagctgccc gccgacggct ttgagttgcg ccaaggtagc
    77341 cgcctgattg ttgataaaac tgcggctttt gcctttggca tcgataatgc ggcggatact
    77401 gagttcttcc ccgccctcat ccaacagccc ttgttcacgc aattctgctt ttaaagcagg
    77461 caaatgggaa atatcgaaca atgccgacaa ctgcgcttct tttgcgccgc tgcggacttg
    77521 gctgtaatcg gccttatcgc ccaacagcag accaatcgca tccaaagtaa tggacttgcc
    77581 cgcgccggtc tcgccggtca aaacggtaaa accgctttga aaatccagat tcagattttc
    77641 gacaatgaca aaatcacgca aagaaagtgt tagaagcatt gtgaggattc cttagcaatt
    77701 ctaccgacaa aagcaatatc aactttagat atacgaatta tatctaagat tttttaaatt
    77761 aaaaataagt ttttccttaa ttttgcttag tgtttgtttt tatctgctta tttgttgcaa
    77821 tgccgtctga agcaatgtgc gtttcagacg gcatttggaa tttcagttgg gcagggtatc
    77881 ggacggtacg gtttccggct cttcttcatc cgtaggcggc atttctatca aatcgggcaa
    77941 aaccagttcg cccagttcgg tcagcggcgg cagttcttcc aaaccgtcca agcgcaaatc
    78001 gctgagaaaa gttgccgttg ttgcccacaa tgcgggtttt cccaatgtgt cccgatgtcc
    78061 gatgacttca atccaccccc gatcctgcaa ggtctgcatc acgttctgcg acaccgccac
    78121 gccgcgtata ccttcgatgt cgccgcgcgt tacgggctgc tggtaggcga taatcgccag
    78181 tgtttccatc acggcgcggg agtagcgcgg cgcacgctgt tcttgcaggc tgcccagccg
    78241 ctcgaatgcc gtctgaacaa tctgaaaacg ccagccctcc tgcgtatgca ccagttgcaa
    78301 cgccctatcc tgccaacgcg ttttcaactg cgccaacaca tcaatcagtt tgtcttgcga
    78361 caacggcggc acacacagtt cgcgcataga tttttcggtc agcggttcgg tttgggtcag
    78421 cagtgcggct tcaatcagcg cgtcgggaga aattttgtcg gtcatacggg tatccgtgct
    78481 gaaacggcat gggtttggat atgccgtctg aaatcggttg gagtagagag aagctgcctg
    78541 aaaaatattt ttcagacggc attctttatg cttccgaagg cttctgccct ttgcgctctg
    78601 cttttctggc ttcgcgaatg gctttgagtt cggcttcttt ctgacgtgct tttttaagcc
    78661 aagtttccca ttggttcggt gtttcaaggg tgattctgcc gattttgcct tcgcggaagt
    78721 ccgtgaggat gttttcggcg gctttttggt agttgacccg tccgccgctg aggactgcgc
    78781 cgcgtttttt ggctatccat tcgagccacg agttgtcgtc ccagtggctg ctggggtctt
    78841 tgtcggcttg gtagcgttct tgcagcatag ggaggtagtg gcggcggagg tagtctaaaa
    78901 gttcgagggc gacttcttct tcgtccaatg cgttgcgtcc gactgcgccg ccggcggcga
    78961 ggttgtagcc gctttcttcg acgatgattt tcggccacag cattcccggg gtgtcgtaca
    79021 gccagaagtc gtcggcaagg aaaaggcgtt gttcggcttt ggtaatgccg ggttcgttgc
    79081 cggttttggc ggattttttg cctatcattc cattgatgag ggtggatttg ccgacgttgg
    79141 gaatgccgca aatcaaaacg cgcaggggtt tgtctatgcc ttgccggtgg ggaatcatcg
    79201 cgcggcaggc ttgggtgatt ttgccgtgtg cgccggtttc ggaggaatcg agggcgatgg
    79261 cccgggtgtc ggggcggctg ttgtagtggt caagccagac ctttgtgcgt tcagggtcgg
    79321 caaggtcttg cttgtttaaa attttgagtt tgggcttacc tttggaaagc tgcgccaaca
    79381 gtggattttc gctggaggcg ggcatacgcg cgtccagcat ttcaatcacc atatcaacgc
    79441 ttttggcacg ctcggcgata gcttttcttg ccttgttcat atggccggga aaccattgga
    79501 ttgccatgtc tgttctttct tctcaatatt tgaaatgccg tctgaaacgg cggacggggt
    79561 ttcagacggc ataatggttt gacgggatta gcggcccgac aggttttgcg cctgcctgtg
    79621 ccgtatcagc aattcataac gccctttcaa ccttcgggcg agtttttcga caatatagac
    79681 cgaacgatgc tgcccgccgg tacagccgat ggcgacggta acgtagctcc tgctttcatc
    79741 ctccaaacgc ggcaaccaac gcgtaacaaa cctttcgatg ccgtcaacca tttcctgcgc
    79801 caacggctgt ccgtccaaat aatcccaaac gggcttgtcc ataccggtgt aaggcctcaa
    79861 ctcgggatcg taatacgggt tgggcagact gcgcatatcg aacataaaat cggcgttgtt
    79921 cggcacaccg tatttgaacc cgaaggactc caaaaccacc agcagtccgg tacgctcaac
    79981 cttcagccac tgccggactg catggcggag ctgttgggca ttcatcttgg aagtgtcgat
    80041 acagtaggcg atttccttaa gcgggaacag ccattcgcgc tcttttttca ggctttccaa
    80101 cagcgtcata tcctgattgc tcaaaggatg tccgcgccgg gtttcggaaa agcggcggac
    80161 cagcacggcc tcttccgcct cgacaaacaa aacttccacc ctgtgtccga gtccgcgcag
    80221 ataggcaatc tgctcgcgcg cttgggcaat gtctatgccg gaacgcacat cgacgctgac
    80281 cgccaattcg gtttcgtccg cacgttcgat atggtacgac accagcgacg gcagcatttc
    80341 caagggcagg ttgtccacgc aaaaatagcc caaatcttcc atttggcgca gtgcaacgga
    80401 tttgcccgag ccggaaagcc cgctaatcag gacgattttc atgattctgt tcgttttctt
    80461 taagttgcgt ctgatggcgt tccaaaaatt cgcgcgtact gtctttgccg cgcaactgca
    80521 aaatgtaatt gcgtaccgcc gcctcaacca aaacggcaag gttgcgtccg acggcgacgg
    80581 gcagcgtaac cgaacggacg ttgacgttga ggatggattc ggtttcggtg cggatgctca
    80641 accggtcgag ctgcttcata tactcgtcgt ccgcctcgac caaattgata atgagttgca
    80701 ggattttttt ggggcggatg gaagtttcgc cgaaaatatg gcggatattg agtatcccca
    80761 agccgcgcac ttccaaaaaa tcgcgcagca taggcgaaca acgcccttcc aatgtttccg
    80821 gaccgatgcg gaacagctcg accgcatcgt cggcaatcag gctatggccg cgcgaaatca
    80881 gttccaatgc caattcgctc ttacccaaac cagaatgtcc ggtaatcagc acgccgattt
    80941 caaacacatc gagaaatacg ccgtgtttga cggacgatgc tgccaaggtg cgttgcaggt
    81001 aaatccgcaa cacatccatc agataagggc tttcgagttt ggaagtcaac agggggatgt
    81061 cgtttttgtg gcagtagtcg cgcagccccg gggaaaccgg caaatcgttt gccacaatga
    81121 ccaaagacat ggaaatatcg aacaggtctc cgaactgata acccgtctcc cccgattcga
    81181 ggcggttcag atattccgac tccgccaaac cgaccacttg gatttggttg ggatggatga
    81241 aattcaggtg tccgaccagg gcgaggacgg gcttgtccgc ctctacgccg atacggttgt
    81301 ccgcacccga attgccggcg gcccaggcaa gttgcaactt gtattggttg tcgtcaaaca
    81361 ggcggcggac ggagatactg ggcatatttt attcttcggt caggatggcg cgggcttctt
    81421 ccgcagaagc aaccgtcatc agcgattctc tgatgctttt ttgggaaaac ttgccggcca
    81481 gtttggataa gacttccaaa tgctcgcccg ttgcgttttc cggcaccagc aagataaaaa
    81541 ccagtgaaac cggtttgccg tcaggcgcgt cgaatccgac gggttcgcgc gtacggatga
    81601 acgcgcccgt cgcctgcttc acgctcgcgt ggcggccgtg ggggatggcg acaccctgcc
    81661 ccaaaccggt cgaaccgagt ttttcacggg caaaaagaca ttcgaaaaca tcagcatggg
    81721 acaatgagga ttcgcgttcc aaaagcaggc ccgcttcctc aaacagcctt tttttactgc
    81781 ccacctccat atccaaaaca atatgggaca aaggcaaaat ttcgccgata aggctcataa
    81841 gcttctcttt tcagacatcg caaaacagaa ggattgtacc gactgccggg gcaaacctca
    81901 atcccgcata cggtacgggc tgacataaca cagcgtttca aaaacatatt ttaacgcttt
    81961 tcggcacaga tagaaatgcc gtccaaagca gtttacggtt cttcagacgg cattgccctg
    82021 ccttatttcg cgaaaaacgt atgggcaaaa atagcggcaa accacaccca accgattctg
    82081 ttgtttgcca aaaacgtttc aaaacagatt tgccggacgc ggcttttgat ggcggcatat
    82141 tggcggtatt gcagcagcag gacgatgggg attgccgtcc aatatgccca tgccgcaccg
    82201 ataaccgcac ccaatactgc catcagcagg gtaaagccgc catgacacag cataacggcg
    82261 gcgatgtcgt aacgcccgaa cgtgacggcg gaggttttga tgccgatttt caaatcgtcc
    82321 tctttgtccg ccattgcata gacggtatcg taagccagtg tccataaaac attggcggca
    82381 aagagtatcc acgcttcaac gggaacgctg ttgcccactg cggcaaacgc catcgggatg
    82441 ccgaaggaaa aggcaagtcc cagataaaat tggggaatcg gaaaaaagcg tttggtaaac
    82501 gggtaagtca gcgcaaggaa cagcgcgggc aggctcatca gccatgtcag atgattcagc
    82561 ggaatcaggc acaatgcggc aagcagacac aaaaatgccg tcagcagcag ggcttctttt
    82621 ttcttgaccc tgccctgtgc gaacggacgg tttttggtgc gctcgacagc accgtcaaaa
    82681 tcgcggtcgg caaagtcgtt gatgacgcag ccggcactgc gcattaaaaa cgtgccgatt
    82741 gtaaacgccg ccaataccgc caaatcggga atgccgtctg aagccagcca caatgcccag
    82801 taggtcggcc acagtaaaag cagcgtccca atgggcttgt ccgccctcat cagtctgata
    82861 taaacgtcca aacgatcgga cagtcgtaaa aataaagggg atttaagatt cataaccctc
    82921 tttcatataa gtcttacacc cttggaaaac taatccataa acttctagtt ccaataacca
    82981 atttgaatta ttgtcgtaaa atcttattat ttttagacca tcttcttggt tctttatttc
    83041 tacttggcat atatttctat tgttatgcga ccaattttga catttcattc ctttaataaa
    83101 gctatttctt aattcaattt caactgtatc ataatctttt agccacagcc cccatttttc
    83161 gatttctaca gaaggctttg tacttgttcg gatagttaaa atacaattag acaagtcagt
    83221 tgaaaaagag actagtgaaa tatccattat gccactagga tatatttgtt ttagaaaacg
    83281 attcctactt attatttcta ttatattcat atttcttact aaaaattata gtgtccgtga
    83341 gtaccatcga catgtcctgt atctaaattg gagtctctta tattaaaatg tggtttagat
    83401 gcaggttgcc gcacagcttg aaaaaacggt attttatccg ataaagcgtt tcagttcggg
    83461 cagaaaatac tcggtcagca gcatttcctc gccttgatgg gaaaaccgag aacgccgcgc
    83521 ggcaaagtac cgtccgcatc cttcactgga aacggcaaac tcaaacgccg aacgcgcccc
    83581 ttccaaatcg gcttgaaaca gacgctcgcc caaagggcgt gtgccgcagt ccaaaatgtt
    83641 ttgccaaaac gccgaaccga cgctgcacgc gcttcgtgcc tgcacaacag cagtgccgtc
    83701 cagtttcaga agcacatcgc gcaccctccg ccgtccgtat tccgtttccg cttcgcccag
    83761 tttcagcagt tccaccgaaa atgtatgcgg caaggcgcac aatgcggcgg tcagcgaccg
    83821 ggctttcaat aaccggctca tcggcaggtc gatgccgtct gaaacggggg cgggcaagtc
    83881 gggcagccat ttcccaaata ggtgttccat atctttccca atctttatac cgcgcctgtt
    83941 tttgccaact ccatccattc cgcttcggga aaatcggctt tcagacggca ttgcagatag
    84001 ttcaggctgt cttgggcgac gtgtgcgtcg cggctgtcgt ggatgtggtt aaacacgagg
    84061 ctgtgcaggc gaatgccgta ttgtttgagc acgacgaaac tgagcagggt gtgattaatg
    84121 cttcccagcc gtccgctggt aacaaggatg acgggataag cctgttgctg aatatgatca
    84181 atggttaaca gcttttccgt cagtggtacc atcaacccgc ccgcgccttc gaccaaaacg
    84241 acttcgtact gcgccgccaa ttcttgtgtg gcggtgcgga ttttgtccaa gtccaaaccc
    84301 ctgccgtcca gtcgggcggc aaggtggggc gaggcgggat ggctgaagat ttcaggcata
    84361 gtcagacgct gttcatcagc ttcctgcatg ggtatgccca tgattttacg gtggacggcg
    84421 atgtcttcgg cgatgtcttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg
    84481 ccctgctgca acaattgttt tgccaacata ccggtggcga cggttttgcc gatgtccgtg
    84541 tctataccgc tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg
    84601 ttttatcggc aagtttggtc aaaatgccgt ctgaaatgat atagggcggc atcagataca
    84661 ccagcctgcc gaacgggcgt acccaaatgc cctgcgccac acaatccgct tgaaaacgcg
    84721 ccatatccac gcctttttcc aactctatca cgccgatagc acccaaaacg cgcacgtctt
    84781 tcacgccgcg aatgtcccac gcggctttca gacggccttt taagatgctt tcgatacggc
    84841 ggatatttgc ctgccagtct tgggataaaa gcagcttgac cgaagcacag gcaacggcac
    84901 acgccagcgg gttcgccata aatgtcgggc cgtgcataaa cacgcccgct tcgccgcgtg
    84961 aaatcgtttc ggtaactttt tgcgaagtga ttgccgccgc cagcgtcata tagccgccgc
    85021 tcaaaccctt gccgatacac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca
    85081 tcttgcccgt gcgcccgaat ccggtggcga tttcgtcaaa aatcaacacg atatcaaatt
    85141 cgtcgcacaa atcgcgcaag ccgcgaagat actgcggatg ataaaaatac atgccgcccg
    85201 cgccttgcac gaccggttct aaaataaagg cggcaatatc cacatgatgc gcttcaaaca
    85261 aggcgcggac aggctgcaaa tccgccccgt cccattcatc gtcgaaacgg tttttcggat
    85321 tatcgacaaa ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg
    85381 gatcacaaac ggacatcgcg ttccaagtat cgccgtgata cccgcgccgt accgtcgcaa
    85441 tattctgctt cgccgtcaga ccccgcgcct gctggtattg caccgccatt ttcaacgcga
    85501 cttctaccga aaccgaaccc gaatccgcat aaaaaatacg gtccagcccc tgcggcaaaa
    85561 tcccgaccaa caacttgccc agctccaccg ccggctcgtg cgtcaggccg ccgaacatca
    85621 cgtgcgccat ttgtttcatc tgattctcaa ccgcctgatt caaaacggga tgattgtaac
    85681 cgtgtatcgc acaccaccaa gacgacatcc cgtcaatcag ccgcgtgccg tccgccaatt
    85741 cgataaacac cccttctgca cgtttgacag gataaacggg aagcggatcg gtcatggaag
    85801 tatagggatg gagcagatgg gtacggtcga aattaagtaa tgatgatgtg tgttgatgtt
    85861 cagacggcat aagtttctct cttttcttat tactgcattc aaacgcaaaa agcgtattct
    85921 actccgacag accgcttccc acacctttcc atccgtttcg ggcgaaaaac cgcgaaacaa
    85981 atcgtccgca acataagcgc acaccgtttc gcattcctca agcctgattg gaatcagacg
    86041 acccaacgcc caataccgtt tcacacagcc tccgcccaac gcttcaatca atcatagata
    86101 aatacgatac agatcatagc aacagccatc gcaacagcgt tggcaaaatc aggggactcc
    86161 ggcataggcg catagcatct gccgatgcac ggctcctcat tcggctctat gaataccata
    86221 cccatcacaa aatccactgc taaaaccagg cacggcttct tatacttatg atagatttcc
    86281 accatcctgt cccatatatc ccaaacactc ataccgtata tcccgcaggc aacaaattcc
    86341 gattgaaggt tacagcccta ttttatagtg gattaaattt atagtggatt aacaaaaacc
    86401 agtacggcgt tgcctcgcct tgccgtactg gtttttgtta atccactata ttttcaaacc
    86461 ggaaaaatgc cgtctgaacc ttcagacggc attccattca tatttattcg tcttttttgt
    86521 gttcgatggc gtttcgggaa cgattgccgc cgtaaggctc ttcaaccgta tattctccct
    86581 cgataatatc gtcatcgtgg aaaaatccct cttttctgcc cgattggttc atgttgaaaa
    86641 aattttccgc acctcctgcc tgcaacactg cccctccctt aaacggcagc agcagcaata
    86701 ccgccaacac cgaggatacg aatcccggac tcatcagaca caccgccgcc accgtataac
    86761 ggataggcca caacatctga taaacagata ccttcccact actttttacc gccgcgccag
    86821 ccaataaaag accggacagc cccgtatgcc tgagcatcag cacaccggcg gcaaaggttg
    86881 ccgccattag aaacagcgtc caaccgccgc ccagccaatc ggcaacccac acaatcgaca
    86941 taatttccaa aaacagcagc accaaaaaac cgataccgaa aaatctcatt gaccgtcatc
    87001 cttatattta agtaaacagc aaaccgcccg aacaggattc caagcgagct gcctgtaaat
    87061 gattacaaaa ccatgtgctt caagccgaaa caatgtgaaa tcacgcaata attattttta
    87121 gcgtgtaaaa caaaaccggc tgcatacctg caaccggtct caaatcagca caactcctta
    87181 tccaaatcct ccaacaggtc tttcagaccg tctccgattt cctgaagcgt aaccggacgg
    87241 ttgccgtccg aacccgattc cgcaccttct gcatacggtg caaacgtgct cttcagtttc
    87301 aacagtttca ccacatccaa acgggtgtag cgttctccgc cgtaaccgac aaccacaccg
    87361 tgatcatgtt gccattgcgc aaaaccatag gggctgattt gcaacagtcc gcacaactcg
    87421 tccagcgtga aatagcgttt tgcaggaatc gtcaaatcaa cgttgtttgt catagtagtg
    87481 ttccaccatg cctttgagtt tctggctggc atggaaagtt accacgcggc gggcggtaat
    87541 cggcacttcc tcgccggttt tcggattacg gcccgggcgt tgcggcttgt cgcgcaactg
    87601 gaaatttccg aaaccggaaa ttttgatttc ttcgccgctt gccaaagtgc tgcggatttc
    87661 ttcaaaaaag agttcgacaa tttctttggc atcgtttttg gtgacgttgc tgactttatc
    87721 caccaaaata tcggccagtt ctgctttagt tagtgtcata tgattacctt ctgttgtaac
    87781 agttgtggat tattaccaat ttttatttaa aattcaagcg aatatttatt ttttaactgc
    87841 gaagccgcgc ccctgcctcc gttgccacgc caatcagttt tccaataagc ggctcgaccg
    87901 cctcatccgt cagcgtgttt tccatatcct gcaaaatcac tttaaccgcc acgctcttca
    87961 tcccttcggg cagacccgtg ccgcgataca cgtcaaacac gctgatttcc tgtaccaact
    88021 tgtttgctgc gcctttcaag acaagcagta aatcatcatg attcataact tccggcatca
    88081 caaacgccaa atcgcggcgc accggctgga atttcgatac ggcccgatag cgcgttttcc
    88141 cgcattccaa cacagccgcc atatcgattt caaataccag cggcgcttgc ggcaggtcgt
    88201 atttttgcag ccatttcgga tgcagttcgc cgacaaagcc gatgactttg ccgtctgaaa
    88261 cgatattggc ggcacgtccg ggatgcaggg cgggatgtcc ggttttaacg aactcgaccg
    88321 ctttgttttt caacagattt tccacgtccg ccttgatgtc gtaaaaatcc gcattgcgcg
    88381 ttttctcacc ccattgttcg ggcatcgccg cgccgtacca cagaccgccg atgcgttcgt
    88441 tttggacaaa ccggccgtct gaacctttgc cgaacacgca ggcgatttca aacacgcgca
    88501 cgcggttttg cttgcggttc aggttgtttt gcaaaatttc cactaagccg ccaatcaacg
    88561 tagaacgcat cacggcatat tgcgccgcca gcgggttttg caggcggatg ggggcggcgt
    88621 tggcggcaaa atcgagttcc cactgctcgt caacgaaggc atagctgatc acttcgcggt
    88681 agccgcgcgc cgccatttcg ttgtaaacgg caaaacgcgg acggcgtgtt tcgggcagtt
    88741 ccgacatttt cagacggcct gacgtgtaat cgtcggggat gttttcatag ccgtaaacgc
    88801 gtccgatttc ttcaatcaaa tcagcctcaa tttcgatgtc gaaacggaag ctcggcgcgg
    88861 taatgcggaa gccttccgcc gttttctcag gctgcagacc caagtgttgc aaaatggttt
    88921 ccacctgttc ggcaggaatg tccacgccca acacggtttt cagacgaccc aaacgcaatc
    88981 cgacctgctt ggcttcgggc aatttacctt gtgcttccac catctcgcct gccgcaccgc
    89041 cgcaaatctg caaaaccaat tcggtggcgc gttcaatggc atccgcctgc aaacggtaat
    89101 ccacgccgcg ctcgaagcgg aacgacgaat ccgaaccgaa accgtattgg cgcgatttgc
    89161 cgacgatgat ttcgggctca aaccaagccg cttccagcac gatattttgc gtgccgtctg
    89221 aaaccgcgct cgccgcgcca cccatcaagc cggccaagct caacgcacct ttttcatcag
    89281 cgaccaccag tgtattgtca gccaaagtaa ccgtcttctc attcagacac gccaaagtct
    89341 cgccattttg agcacggcgg acaatcaaac tgcccgacag cttatcagca tcgaaaacat
    89401 gcataggctg accgatttcc agcatcacat agttgccaat gtccaccaat gcggaaatgc
    89461 tgcggatacc gctgcgttcc aaacgttgtt tcatccaatc aggagtagcc gctttcgcgt
    89521 tgacattttc aataacgcgg ctgataaaac ggccgcagtc agccggtgca tcaatacgga
    89581 cagcctgttt tttctcactg ccgatagggg ccgtctgaat ttcaacaggc gtaaacgcac
    89641 attgagtcaa cgcagaaacc tcacgcgcaa tgcccttaac actcaagcaa tcagcgcggt
    89701 taggcgtaat tttcaacgta aacagcatat cgtccaaatc caagtattcg cggatgttgg
    89761 taccgacagg cgcatcttca ggcagaatgt gcaggccgtc tacaccatca tcaggcaaac
    89821 ccagttcatt ggtcgaacac agcataccgt ttgatggtac gccgcgcatt ttagtcggct
    89881 taattttaaa attgcccggc aaaacggcaa ccggcaacga acacggcact ttaatgcccg
    89941 gcttgacatt cggcgcacca caaacaatct gaaccaactc gcccgtaccg gcatcaactt
    90001 gagtaacgtt caaacggtct gcatcaggat gtttttcaac agattttact tcggcaacaa
    90061 ccacgccact gaaagcaggg gcggcagtat cgatttcttc cacttccaaa ccggccatgg
    90121 tcaaaagatg ttccagttta tcggcagaaa gatcaggatt ggcttgggtt ttcagccatg
    90181 agtaggagaa ttgcatgtta tgttctcaat caattaatta acttttgttt tatcaatttt
    90241 cgttcagaca gtctgtttta gaattctggt taatgttgat atttactcaa ctctgaaaaa
    90301 tattttctct gtgaaatttt aaaaattcca gacttgggtt tagtctttca ggcagtctta
    90361 tcatccgatt ttcatatggt ttaaataaat catcaaatcc ttctgttttt gccagtagtg
    90421 gagaaaatat aattttgaaa ttttcatcta ttcctaacaa ccccctatca aaagctttgt
    90481 catgtaacgc attcaaacat agcccattcc ttgggtttaa acggttatcc ttatcttccc
    90541 cccagggttt gatatgacta gcaactaaca attcaggttg ttttaatcca gtaatacaac
    90601 attgattatt gtatgctgcc aaaacactgg aacggaaaaa gccctgattg acgcgtactg
    90661 atattcttgc aaacctttcc cttccctttg gaatctcagg tgattctatt tcaatattac
    90721 tattctcatc atttgattgg tattgggaaa tgactccctc aaattcttta ttaagttgct
    90781 cccaatctcc tgaaaactca ttccatacga caacatccat tttactgcca ttacttaatc
    90841 ccgaaatatt tttgactttc agcgtaggat ctaaacgacc caaatttcct atcttcatac
    90901 cgagtgcgga cggtgtcctg cccagtattt cagcatattc ttgaataaca ggattgtcct
    90961 tactaacctt ttgaaaagga atgatgtaat aaagataaag ggcaaccagg gtttcatctc
    91021 tagtccattt tttcattttt atcagctatc tttctgattt tccataagga aatcgtacaa
    91081 tacagatttt cttattccct gaacatattg ctgtccatct agtactaatt tgtataggta
    91141 tacatcataa tcttttaact ccttctcgct tgccaataac cagtcgtcta tttctgaaga
    91201 aaaatagtca ataatttctt gtacctgttt atcagataaa ttatttatat ttattttatt
    91261 attcaaaata taatcagaaa gcctcatatt tctaaaatct ttattttgat aaggtaaacc
    91321 cacacagcac atcaaattgc ttcggaatgt attgtttgat ttcttccaaa gtaatatcgc
    91381 caaacggcac ttctccgaaa ttggcttcgt acgtcagttt ggctttttcg tcccactcgc
    91441 tcgaaaacac gtattccccg cccaagttct gcatcgcgat gcggaagccc ccgatacctg
    91501 cgaacaagtc gataaaggta aatttgggtt tcaaggccgt ctgaaaagta tcgttgtttt
    91561 caaaaaggcg gtattgtgct gctttttctt ttaaccgttc atcctgccca tcttccttaa
    91621 tcttgttttt gtaaatcaat tcatcttgtt tggatgattt ctttgacttt ttcggcacag
    91681 caggttgtat ttttgacgac atcgttactc caaaaacgta aaaccgtcca gccctcggct
    91741 ttcagacggc ctgttacttc tatatccctt tggatattgc gctcaatttt ggcaatccaa
    91801 aactcacgat ttccctttat taccgctttt ttctgttccc aatccttacc gtgccagaat
    91861 tccccatcga caaatacggc aactttatat tttttaaatg aaaaatcagg ttttccaaaa
    91921 atgcttccgc tatttttccg atacctgagc cccaaagccc acattgcctt cgccaacaca
    91981 agctcgggtt tcgtccctgt acttttgttg gactgcatac attttttgcg ctgctccggg
    92041 gttaatttat ccattgggtt tatttgagat ttattcaaac tgcttcaaga agttcaaatc
    92101 gttatcgaag aacaagcgca ggtcattgac gttgtagcgc aacatggcaa agcggtcgag
    92161 accaataccg aaagcgaaac cggtatattt ttcagggtcg atattgacat ttttcaacac
    92221 attaggatgt accataccgc aaccgcctac ttccaaccat ttgccgtttt ctcccatgat
    92281 gtcgatttcg gcagaaggct cggtaaacgg gaagaaagac ggacggaaac gcacttgtag
    92341 gtcatcgcgt tcaaagaagc gacggataaa gtcagtgaac actgctttta agtcggcaaa
    92401 agttacgccc tcttctaccc acaaaccctc ggcctgatgg aacataggcg agtgcgtggc
    92461 atcgctgtcc acacggtaaa cgcggccggg ggcgataatg cggatgggcg gctctttttt
    92521 atcgagcata tagcggattt gaatcgggga agtgtgcgta cgcaaaacat cgccgttttc
    92581 aacgtaaaac gtatcctgca tcgcacgggc aggatggttt gcagggatgt tcagggcttg
    92641 gaaattgtga aaatcgtctt cgatttcagg cccgtccgcc acttcgaaac ccattccgtg
    92701 aaagagttcg accacacgtt gcaaggtcag ggttacggga tgcaggctgc cgccttcctg
    92761 agcgcgtccg ggcagggtaa tgtcgagggc ttcggcggca agtcgggctt gcagcttggc
    92821 ttcgttgagg gcatcgcgtt tggcattaaa agccgtctga aaccggtttt tgcattcatt
    92881 gatatgcgca cctatggttt tgcgctcttc aggcgacatt tgccccaaag ttttcagaag
    92941 tccggtcaac tcgccggttt tgccaagata acgggctttg atttgttcta gagcgttgaa
    93001 gtcttgcgca gcttctactg cggcaatgcc ttctgcaacg atacggttta cattttccat
    93061 aatatcaagc ctgtcaatta atgatatgcc acctgaaaca acgaggccgc ctgtatagcc
    93121 tgacatatca gactcttcag acgaccttat tttcttcacg gccaatccat tggataaatt
    93181 ttctgtattt taacgcaaat ttatttaaaa aaatagactt taaagaaaga aaatcaatca
    93241 catagaattt ccgcaaacaa agaaaaaagg aagccgcagc ttccttttca attttttgga
    93301 ttaagcagcc aaagcagctt tggctttttc aaccaattgt gcaaaagcgg ctttatcgaa
    93361 cacggccaaa tcagccaata ctttgcggtc gatttcgata gaggcgcgtt tcagaccgtt
    93421 cataaatttg ctgtaagaca acccgttttc acgcgcacct gcattgatac gaacaatcca
    93481 caattgacgg aattggcgtt tgcgttggcg gcggtcacgg tatgcgtatt gaccagcttt
    93541 cattaccgcc tgcttggcaa cgcggtaaac gtttttacga cgaccacgat agcctttggc
    93601 taacgcgaag attttttggt gacgggcacg ggcggtaaca ccgcgtttta cgcgtggcat
    93661 attctaaact ccttaagcgt agggtaacat tttagcaaca gaagccaaat cgcgatcatt
    93721 taccatagag gtaccgcgca gttggcgttt gtttttggtg gtctttttag tcaagatgtg
    93781 gcgtttgaac gcatgagcgc gtttcacacc gccgttaccc agtactttaa agcgtttttt
    93841 cgcgctagac ttggttttca ttttaggcat gggaaaactc cattcgttat cggataaggc
    93901 attagggggt tttaaaacat cggtttcaaa cacttgaacc cacaatgcca cggttttttg
    93961 ccgcaattga aaacttactc cgaagcggca atcggagtaa gcggaaaatt atagctttat
    94021 tttttcttcg gcgcaatcat catcaccatt tgacggcctt ccattttggg aaaggactcg
    94081 atttgcgcca cttcagccaa atcttctttt acacgttcca aaagttgcgc gccgagttgc
    94141 tggtgagcca tttcacggcc gcggaaacgc aatgtcactt tgactttatc gccgtcggca
    94201 aggaagcggt tgatgttgcg catcttgatt tgataatcgc cctcatctgt acccggacgg
    94261 aatttgattt ccttgatttg cacctgcttt tggtttttct tggcttcgtc gcgcttcttg
    94321 gcttgttggt atttgtattt accgtaatcc atcagtttgc acacaggagg tttagcagtt
    94381 ggggaaatct ctaccaaatc gacatcctgc ccttcggcca tagccaaagc ttcacgaact
    94441 gaaacgacac caagctgttc gcctgactca ctgattaaac gcacttcttt ggcggtaatt
    94501 tcaccgttga ttcgtgcttc gcgttcttga gcgatgatga atactcctat aaaaattaat
    94561 gattgacgag ggcatcagtg atttcttgct gcaattgcgc aatgaaatca tccaaatcca
    94621 aagaacccaa gtcttccgct ttgcggcgta ccgcaacttt gttttcttgt ttctctttat
    94681 cgccgataac gatttgataa gggaaacggt attggctgtt gtcgcggatt ttgtaaccga
    94741 ttttttcatt acgcaaatcc aactcggcgc ggaagcctgc cgcctgcaat ttggcagcaa
    94801 cttcccgaca ataatctgcc tgattttcgg tgatgttcat aatcaccatt tgaaccggtg
    94861 caagccataa cgggaaagag cctgcatggt tctcaatcag aatgccgaca aaccgctcca
    94921 aagaacctaa aatggcgcga tgcaacataa caggccgcgc tcggtcgttg ttttctgtta
    94981 catattctgc atccaagcgt tccggcaaga cgaaatccag ttgtaatgta ccgcattgcc
    95041 aagaacgacc caaggcatct ttgacgtgat attcgatttt aggcccgtaa aacgcaccct
    95101 cgcccggcaa ttcgccccat tccacgccgc aggcagtcaa tgcctcgcgc aaaccctgct
    95161 ctgccttatc ccacacgtcg tctgaacctg cgcgtttttc agggcgaagg gaaagcctga
    95221 cggatacatc atggaaaccg aactgtttgt agatgcgaac caacaattca ttgaacgcac
    95281 gagcctcgct gacgatttga tcttcggtac aaaaaatatg cgcatcatcc tgcacaaaac
    95341 cgcgaaccct catcagaccg tgcagtgcgc cgctcggctc gttgcggtgg caagaaccga
    95401 attccgccaa acgcatcggc aaatctcgat atgaacgcaa accgttgttg aaaatttgaa
    95461 catgacccgg acagttcatc ggtttaaccg catattcgcg tttttccgaa ctggttacga
    95521 acatattgtc tttgtagttg tcccagtggc cggatttttc ccaaaaggtt ttatccataa
    95581 tttgaggcgt tttgacctct ttataaccgg cggcgttcag ctctttacgc atatgctgct
    95641 cgatagtccg ccacaaagcc caacctttgg gatgccaaaa caccataccc ggcgcttcgt
    95701 cctgcaagtg gaacaaatcc agttgcttgc ccaatttgcg gtggtcacgt ttttctgctt
    95761 cttcaatgcg ttggatatag tcttttaatt cgtctttcgt cgcccaagca gtgccataaa
    95821 tacgttgcag catttcatta ttgctgtcgc cgcgccaata tgcgcctgcc agcttggtca
    95881 gcttgaagtt tttcaggaag cggatgttcg gaacgtgcgg accgcggcac atatcgacat
    95941 attcctgatg atgatacata cccattgctt caacttcggg catatcttca atcaggcgca
    96001 gtttgtattc ttcgccgcgc tcttgaaaaa ttttaatcgc ctccgcacgc ggagtcatga
    96061 ttttgaccac atcataatct tgggcaatca attctttcat acgcgcttca atggcggcaa
    96121 catcttccgg tgtaaacggt ttttccgtgg cgatgtcgta ataaaagccc tcttcaatga
    96181 cggggccgat aaccattttt gcattaggat agagttgctt gacggcatgc ccgacaagat
    96241 gtgcgcagga atggcggatg atttcgatgc cttcctgatc tttcggagta atgatttgaa
    96301 cagcagaatc ctcaacaatc gggtcgcacg catcgaccaa tttgccgttt accttgcctg
    96361 ccaccgccgc cttcgccaaa ccggcaccga tagacgcagc aatttgagcc acggtaacgg
    96421 gggattcgta ttggcggact gaacagtccg gcaaggtaat attcaacatc aaacgctccg
    96481 aaagattaaa aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt
    96541 cgaaccaacg acccccacca tgtcaaggtg gtgctctaac caactgagcc acgtgccttc
    96601 aaagaatttt tgtattttat cgggtcgttt taatttttgc aagagagatg tgcatttctt
    96661 tatgcaacat tgtggcaaga tttggggata ccgtctgaaa aaccgtggga attcatttat
    96721 aatgccgtct tttaaaatat atttcgaccc ctgtcgccca acttacattc aatcttatgc
    96781 ttaaatttac tttacacaaa aaagacggtc tcgcccgacg cggcacgctg gagttgaacc
    96841 acggaaaaat cgaaacgccg gttttcatgc cggtcggtac ttacggttcg gtcaaagcga
    96901 tgaacccgca aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt
    96961 tgtggctgcg tccgggtttg gaggtcgtcg aacagtttgg agggcttcac ggttttatcg
    97021 gctgggacaa accgattctg accgactcgg gcggttttca ggttttttct ttgtcggata
    97081 tgcgcaagct gaccgaagaa ggctgtacgt ttaaaagccc gattaacggc gacaagctgt
    97141 ttttatcacc tgaaatttcc atgaaaatcc aaacggtttt gaactcggat atcgcgatgc
    97201 agttggacga atgcacgccg ggcgaaacaa cgcgcgaaca ggcgcgaaaa tccctgcaaa
    97261 tgagcttgcg ctgggcggaa cggagcaaaa aagcctttga agatttgaaa aacccgaacg
    97321 cgctgttcgg catcgtgcaa ggcgcgatgt atgaggattt gcgcgaagaa tctttgcgcg
    97381 gtttggagga atttgatttt cccggccttg ccgtcggcgg tttgtccgtc ggcgaaccca
    97441 agcccgaaat gtaccgtatg ctgcacgccg tcggcccgat gctgcccgaa cacaaaccgc
    97501 attacctgat gggcgtaggc acgccggaag acctcgtgta cggcgtggcg cacggcatcg
    97561 atatgttcga ctgcgtgatg cccacccgca atgcgcgcaa cggctggctg tttacccgtt
    97621 tcggcgattt gaaaatcaaa aacgccaaac acaagcccga caagcgtccg atagacgaaa
    97681 gctgcacctg ctacgcctgc caaaacttca gccgcgccta cctgcaccat ctgcaccgcg
    97741 ccggcgaaat cttgggcgcg caattgaaca ccatccacaa cctgcatttc taccaagtca
    97801 ttatggcgga aatgcgcgat gccgtcgaac aaggcaaatt tgccgactgg caggcgcaat
    97861 tccacgaaaa ccgcgcgcgc ggcgtggact gattcccggc aatgccgtct gaagccgctt
    97921 cagacagcat tttgccgctt taccctacct gcactatttt tcagaaaccg atcatgcttc
    97981 cattccttcc agaaccctcc ctttcctata cgcaacaaaa ccgtaccgcc gttttgctgt
    98041 tgaacctcgg cacacccgac gcaccgaccg cacaggcggt acgcccttat ctgaaatcct
    98101 ttctgaccga ccggcgcatc gtcgaactgc ccaaatggct gtggtatccc attctgcatg
    98161 gcctggtact gacgttccgc cccaaaaaaa gcgcgcacgc ctatgaaaaa atctggttta
    98221 aggaaggttc gcccctagaa gtttacacag cacgccaagc tgccgcgctt gccgaacgta
    98281 tgcccgacct catcgtccgc cacgccatga cttacggcaa tccttcgata gcggatgtat
    98341 tggcggagct gaaatcccaa ggtgtaggca gattgcttgc gattccccta tatccgcaat
    98401 ataccgcttc cagcagcgga gcggcggtag ataaagtgtg cgaacaactg ctgctgcaac
    98461 gcaaccaaat gagcgtccgc accatttcgc gcttttatga tgatgccggc tacattgatg
    98521 cgatgaaaaa ccacatcctc cgatattggg cggaacacgg gcgtgggaaa aaactgatgt
    98581 tgagttttca cggcgtaccg cagaagcact acgacctcgg cgacccctat cccgacgagt
    98641 gccgccacac cgccaaactg cttgccgaag cactggaact gaccgaagac gaatataccg
    98701 tatcgttcca aagccaattc ggcagggcaa aatgggttac accgagcacg caggatttgt
    98761 tcggcaaact gcccaaacag ggggtaaccg agctggacgt attctgcccg ggctttttgg
    98821 cagactgctt ggaaaccatg gaagaaatcg ccctgacggg gcgggaacag ttttatgaag
    98881 cgggcggaaa aaactaccgc tacatcccct gcctcaacga caaccccgac tggatagacg
    98941 cactcgtcgc acttgccgaa gaaaaccttg gcggctggcg ttgaaactgt ttgagccatg
    99001 tctaaaatgc cgtctgaacg ccgtgttcag acggcatttt cccgctactt tagcgtatga
    99061 tggcatctat gccggacagc acatcaagtg caaccttact accgattcgg caaaaaaacc
    99121 tttcacccgc tacgcaaaat caacaacaag gtttcaaaca ttaaggaaac gccaatgaac
    99181 acaacccgac tgccgaccgc cttcatcttg tgctgcctct gcgccgccgc ttctgccgcc
    99241 gacaacagca tcatgacaaa agggcaaaaa gtgtacgaat ccaactgcat cgcctgccac
    99301 ggcaaaaagg gcgaaggccg cggaaccatg tttccgccgc tctaccgctc cgacttcatc
    99361 atgaaaaaac cgcaggtgct gctgcacagc atggtcaaag gcatcaacgg tacaatcaaa
    99421 gtcaacggca aaacctacaa cggattcatg cccgcaaccg ccatcagcga tgcggacatt
    99481 gccgccgtcg ccacttatat catgaacgcc tttgacaacg gcggcggaag cgttaccgaa
    99541 aaagacgtaa aacaggcaaa aggcaaaaaa aaccaaacag acaaaatgcc gtccgaaacc
    99601 ggcaatccgg cttcagacgg cattcaaacc aaacctttct aacgcggtac tttcagaccc
    99661 atagacccga tattgccgtc cccgtcaacc tccttcgcgg tcagcataaa gccgccgatg
    99721 tcaatacggt cgccctcgac cggctcctga ctgtcggaac gcttatcgaa aagctcggca
    99781 aggctcaaac cctcttcgcc cgcttccagc ttcaaaccgt aagcaagtgc caaatcgccc
    99841 gaacgtgctg ccggcgaaac gacaaactca ccgaagaaat caaaattttc acggacgccg
    99901 atacccgtct cggtaaagta tttcgccatt ttatcgacct tgccgtcggg caggatgtac
    99961 cacgccaaat cctccgcctg aagcacggtg tcggactgca tttcgattcg gctgccgttg
   100021 cgtatcaatg cgaaacagcg tgcgtcaaac gaatcggaaa tcggttcgac cgtatcggga
   100081 tggtgtccct ccgcttcaga ttcggcaacc actttaaacg ccgacattct gacaatttcc
   100141 ttttccgcca gccaaatatc atgcgtatct ttcggttcgg gcttgttggg catggcaacc
   100201 ttcaacagcc gcgccatcac aggaatcgtc gttccctgaa tcagcagcga cagcaccacc
   100261 acggcaaacg ccacatcaaa cagcaggtgc gaattgggaa cgcccatcac cagcggcatc
   100321 atcgccagcg aaatcggtac ggctcctcgc aagcccaacc aactgatata cgccttttca
   100381 cgcaggctgt aattgaattt ccacaaaccg ccgaacactg ccagcggacg cgcgaccagc
   100441 atcaggaacg ccgcaatcgc caaggcttcc gccgccctgt ccaacacgcc ggcgggagaa
   100501 accagcagac cgagcatgac gaacaaagtt gcctgcgcca gccaagccaa accgtccatc
   100561 acacgcaaaa cgtgttccgt cgcacggttg cgctggttac cgacaatgat gccggcaagg
   100621 taaaccgcca aaaagccgct gccgcctatg gtattggtaa acgcaaacac aagcagcccg
   100681 cccgacacaa tcatcagcgc gtacagacct tccgcaagat tcagacggcg taccagcttt
   100741 gccaatatct ttccgcccgc ccaacccgtc agcagaccga aaccgatttg cagcgcaagc
   100801 atccggacaa acgctgccgc acccgattcc gccggctgca taatcatggt aatcagtgcc
   100861 gtaaccaaaa aaaccgccat cgggtcgttc gcacccgatt cgatttccaa agtcgcctgc
   100921 acccgttcgt tcaaacgcac gccgctgttg cgcaaaaggc tgaataccgc gccggcatcg
   100981 gtcgaaccga caatcgccgc catcagcacg ccgaacttcc aatccaaacc gagataaaaa
   101041 gttgcaaaca gtcccagcgg aagcacggtg gcaaacacgc cccacgaagc gagtaccgac
   101101 gcgggcttca acgcaatccg aaaactggaa agctgcgtcc gcaaaccgcc gtcgagcaga
   101161 ataaccgcca aagcaagctg gctgatgaaa ttcgccatca cgacattatt gaaggcaatg
   101221 ccgccaacgc cttcctcgcc cgccagcatg ccgacggcaa gaaacaccag cagcaaaggc
   101281 attcccaaac gtgcggacaa agtggtcgaa accacgctca ggaacaacag tgcgccaccc
   101341 agcagaaaca ggctgttcat ccaatccacg ccaacggctc ccccttaaaa ttcatgaaat
   101401 tagaatcgaa agattgtaac acggcatttt cttctcacga aactttattt catgtccgcc
   101461 accgccttca gacggcattt tcctgtttca tgccgcctga taccgcccaa acccgcgttc
   101521 tcctatataa tgtcggactt tgcgccgcat ttcaggattt tttatggcat ttgcctcact
   101581 atttactctg ccggactaca tcaccgccgt tttggacgat gttgccctga tgaccaaagc
   101641 cgccgcaaag aaaaccgccg gcgtggccgg cgacgatttg gcacccaatg ccaaccgggt
   101701 tacaggcgta tcggcagaac gcgagttgcc gattgtttgg ccggtggcga aaggttcttt
   101761 tgccaacaag ccggttttgg tgtctgcggc tctgctgctt tccgccttcc tgccgcaact
   101821 cattacgccg cttttgacgg caggcgggat ttatttgtgc tttgagggcg tggaaaagct
   101881 gctgcacaag tttctgcacc gacatgacgc gcacgatgac ggcggcgaaa cggcggttaa
   101941 cgagcaggct aaaatcaaag gtgcaatccg taccgacttt atcctgtccg cagaaatcat
   102001 catcatcgcg ctgtgcgtgg tcgaacaata cagcctgatg acgcgctcgc tcgtgatggc
   102061 ggcaatcggc atcggcatga ctgttttggt gtacggaatc gtcgccgtca tcgtcaaact
   102121 cgacgacttg ggcatgctgc tgatgcgccg cccacaacct cggccttttg cacgatttcc
   102181 tgcacgcgca acactggaac gctggctgga cagagtattc cgccaacctc gttgtcgggc
   102241 cgctttccgg ttcgattgcc tgcgccccgc cttgtcgctg atgaatcgtt tcggcaggca
   102301 ttgattcctt tttcaaatac cgatgccgtc tgaaagacgt tcagacggca tattccgaac
   102361 agacagacga atatgttttc caaactggac aaatactggc agcaccccgc cctctactgg
   102421 cctttgctca tcctttttgc cgccgccacc cccttcgcct tcgcacccta ctaccacttt
   102481 tggctgatgc ccttgatttt cggcgccttc gtccgcctca tcgaactgcg tccgcgtttt
   102541 gccgcctctt ccgcctacct gttcggtctg accgcataca cggcacagtt ctactggata
   102601 cacactgccc tgcacgacgt ttccggcctg cccgacctct atgccgtacc gctgaccttc
   102661 ctgctgcccg cctaccttgc cctttatccg gcactgtgtt tctggctgtg gaaaaaattt
   102721 accctgcccc agggcataaa gaccggtctg gtactgccca tcctgtggac gctgaccgag
   102781 tttgcccgcg agcgtttcct gaccggattc ggctggggcg caatcggcta ctcccaaatc
   102841 accccggaca gcccgctggc gggtttcgcc cccttgggcg gcatccacat ggttacactg
   102901 gcaaccgcct ttctcggtgt ctggctggtt ttggcgatcg acaacaccgc acgttcgggc
   102961 aaacgcctgc ttccggccat tctgattgcc gccctgcttg ccgcaggcta caccgcccaa
   103021 caaaccgact tcacccgccc cgacggcagc cgcagcaccg tcgccctgct tcagggcaac
   103081 atcgcccaaa ccctcaaatg gcgtgaagac caagtcatcc cgaccataca gaaatattac
   103141 gaacaggtcg gcaaaaccac tgccgacatc gtcatcctgc ccgaaaccgc cattcccatc
   103201 atgcgccaaa acctgccgga aaacatactg gcgcaattcg ccgaacaggc gcaaaacaac
   103261 ggcagcgcgc tcgccgtcgg catcagccaa tacacttcgg acggcaacgg ttacgaaaac
   103321 gccgtcatca acctgaccgg ctatcaggaa aacaatcagg acggcatccc ttactacgcc
   103381 aaaaaccacc tcgtcccctt cggcgaatac aaaccgctgc ccctcctgac cacaccgctt
   103441 tacaaaatga tggacatgcc cctttccgac ttccgcaaag gcggcggcaa gcaatccgcc
   103501 atgatgatga aaaaccaaaa aatcgccttc aacatctgct acgaagacgg attcggcgac
   103561 gaactgattg ccgccgccaa agacgccaca ctgcttgcca atgtcagcaa tatggcgtgg
   103621 tatggaaaat ccaacgccat gtaccagcac ctccaacaat cgcaggcgcg ggctatggaa
   103681 ctcggacgct atatggtccg cgccaccaac accggcgcaa ccgccatcat ctcccccaaa
   103741 ggcaacatca tcgcccaagc ccaacccgat acggaaaccg tattagaagg acacatcaaa
   103801 ggctacatcg gcgaaacgcc ctatatgaaa accggcagtt catggtggtt gatgggcata
   103861 ttgaccctag ccgcactgat tcttttcatc ttccgaaaca aagaacactg atacctgcac
   103921 agtccgtccg gacgggctgt cttttttcaa accggccgtt tacatacaac cacgtatgta
   103981 atataattct aaaatcgaat tgagctatca ggtttacaaa atcaatcaaa cttgccttac
   104041 aaaattttcc tgtctttcaa accttagcgt atatctcctg ccaaacatac aaaatacaag
   104101 ccaagcagtg atatactgca tcccaacaca ccaaaccgcc gatacgcagt ttcagccgaa
   104161 aggaaatgcc ccaaatgaat aacgctttcg cattacccgc catccaaagc ggcaacggca
   104221 gcctcgaaca atacatccac accgtcaaca gcatcccgat gctgtcccag gaagaagaaa
   104281 cccgcctcgc cgaacgccgg ataaagggcg acctcaacgc cgccaaacaa ctcatcctgt
   104341 cccacctgcg cgtcgtcgtt tccatcgcgc gcggctatga cggctacggg ctgaatcagg
   104401 ccgacctgat tcaggaagga aatatcggtc tgatgaaggc ggtcaaacgc tacgagcccg
   104461 gtcgaggtgc acgcctgttt tcatttgccg tgcattggat taaagcggaa attcacgaat
   104521 tcatcctgag gaactggcgc cttgtccgcg ttgccaccac caaaccgcaa cgcaagctgt
   104581 ttttcaacct gcgcagcatg cgtaaaaacc ttaatgtcct gtctcccaaa gaggcacaag
   104641 acatcgccga cgatttgggt gtcaaacttt ccgaagttct ggaaatggaa cagcgcatga
   104701 cgggacacga catcgccatc atggcagaca acagcgacga cgaggacagt ttcgccccca
   104761 tcgactggct tgccgaccac gattccgaac cgagccgaca actatcgaaa caggcgcatt
   104821 acgccctgca aaccgaaggc ctgcaaaacg ccttggcgca attggacgac aggagccgcc
   104881 gcattgtgga aagccgctgg cttcaagacg acggcggact gacgctgcac cagcttgccg
   104941 ccgaatacgg cgtatctgcc gagcgcatcc gccagattga agcgaaagcc atgcagaaac
   105001 tgcgcggttt cctgaccgaa gaagccgaag cggtttgaaa tatacacaat aaaatacccg
   105061 acaaaacaat ttgtcgggta ttttatttgg taaacatcaa tgcggtttgt gcgtcgtgcc
   105121 gtacacgatt ttatccgccc aagccatcgc aatcgccaaa atcaaaaagc agacatgcac
   105181 cagacactgc cacatcagct gcttttccga cagattggcg gaattgataa atgtttggag
   105241 taaatggatg gatgaatacc gataatcgac atcgacagcc ttaccttcaa taccggtgca
   105301 ttcacatggc tcaaccactc gggccggtca ggatggtcgt cgatacgcaa ccgggatacg
   105361 aacgactcat acccgccaat ctgcaccatg gtcagcaaat tcgcaatcat aaccacatca
   105421 atcagattta aaacagcgag catgatggcg ttttcgtccg acacatccaa attcatgacc
   105481 agatacttca acgatttcaa aaacttatag gcacaaatcg cccgtacaac agtcagcccc
   105541 gcataaatcg gcacttgcag ccaacggctt gcaaaaatca ttttggcaaa aaacccatag
   105601 cctgttcggg ttttggagat tttgcttgat acatttccct tccttgcagc aacaaaagcg
   105661 cgtgattcca atctaccgct gcgaaatctt caatttttca cacccgaaaa cgcttgccgc
   105721 catacagatg tgatatattg attccatttt aacgaacttc ggagaacgac atgagcagcg
   105781 gaaatatccg catccacagc atgaccggct ttgccaacgc ggcggcagag tgcggcagca
   105841 aacgcatcaa cctcgacatc cgcgccgtca accaccgctt tttggacatt caaatcagga
   105901 tgcccgacga tttgcgccat ttggaaaaca gcatccgcga gcagatttca tcccatctgg
   105961 cgcgcggcaa agtcgaatgc aaaatccaaa ttcaagatgc ggaaaacggc aatcagtctt
   106021 tggaactgaa tcgcgttctg gtcggacagc ttgccgaaat caacaaaaac ctccgcaaac
   106081 attatgattt ggcaaaactg ggcgttgctg atattttgcg ctttccgggc gtattggcaa
   106141 gccaaaagga aaatacggaa gcattggcga aaaccattgt cgaactggcc gacaaagcac
   106201 taaaagactt taccgccgca cgtcggcgtg aagggaaaaa attgggcgaa cacctgttac
   106261 aacgcattaa aaacatggaa gaaatcatcg atgctttgag cgaaatcttc cccgcgctgg
   106321 tggaagcgca taaagaaaaa atccgcaccc gccttgccga agccgtcggc agcatcgaca
   106381 acgacaggct gcaacaggaa tttgccctct ttatccaaaa atccgacatc gacgaagaat
   106441 tcagccgcct gcgcacccac attgccgaag tgcgccgtat cgttaccgaa cacaaaggca
   106501 gcagcggcaa acggttggac ttcctgatgc aggaactgaa ccgcgaagcc aacactttgg
   106561 gcagcaaatc catcgctacc gaatgcaccc aagcctcggt cgagctgaaa gtcctgatcg
   106621 aacagatgcg cgaacaggtg cagaatatcg aatagccctg cctttctctc caaacatgac
   106681 aatgccgtct gaaacttttt tcagacggca ttcttgatcg gctgaaaacc tctgttgcaa
   106741 gcaattcaag attgttcttt cacatccacc aacctcgttc cggcaaggaa gtcatacaga
   106801 aactgccggt cgggattcag cagggcgaac ccccacggca aaatcagcca aaccaatgcc
   106861 gcgccggccg cgcccttggg cggaatgccg aggaagtggc gcaatccggc ataagcaagc
   106921 ataggaatga agacgatgaa tatgcacgcc caaataaagc gcaggcgcag caggtgcaaa
   106981 gacggctgta tgccgttaag gtcgcacaag ccgattttcc atgtcctcat cgccaaggtc
   107041 tgcccctgac cgtgccagtt ggcgcggaaa taaagccacc atgctcccat tatcaggata
   107101 ctcgttacca atgcagaaac cgcgatagaa acgggattca gaaaaatggc ggcaataccg
   107161 gcaagcaatg ctgccaaaca ggtcgccgca ccgaccagca gcatctcata cagtatggcg
   107221 gcaaagcggc gtttgagcgg ggcggttttc agtatcatca tcttttcaat cggaatatgc
   107281 cgtctgaaac gccaacagac ttcagacggc atttgtgcaa agatgccttg cggcggttaa
   107341 cggtgcggac gtttctgacc gcgcgactgt tttgccgcca tttgattgat ttcctgacgt
   107401 gtcgggcgtt ttttgaacat agcgtgcgcc tcgtccaccg aacatttttg cgacaggcag
   107461 atcagggtaa tcgcatcttt ctggtgttgt ccgccgctgt ggtacatacg gcgcaggttt
   107521 gcctcgttga ttctgccttt ttcgggatcg atgtcgaatt ttttcagacg ttcgataaac
   107581 tggcgttgct gtcggcggga tgtccaaaca aagccgcccg cagcggcggc gacgatgacg
   107641 gcggcaagca ggatggcaag cggccaggaa tcgacaatca atacgataat gaggcttaaa
   107701 acggcggtgg tcagtaaaat caggccgccc aagcgcagtt tggtcttatt gtccataggg
   107761 ttgatgttcc tttattgagg tggtcgtgcc gtctgaaatg ttttcggacg gattggtgaa
   107821 tacttttcag gattaacggt attttacagt attttcggat tttggggatt accgctttat
   107881 tccgccagcg acacgacaag ctgtttgaat accgtccatg cgtcgccgtc ttccgcgcct
   107941 ttgatgattc ggtcgatttg ggcgcaggtt ttgagcgcgt caagcaggcg gacgacggaa
   108001 atccgcttga ccgcaagcgg tgcgagcgtc tgtttgtcgc cccaaagcct gaggctgttg
   108061 cggacggatt ggatgctctg cccctgtttc agggcagcgg caagccgaat cagcgtccgc
   108121 acgtcttcgg caaccgccca cagaagcagc accggttctt cgccttcttc ctccaacccg
   108181 tccaacagcc tgcatacgcg cgggacatcg cccttcatcc acgcgcctgc cagttggaac
   108241 gcgtcgaagc gggcaacatt ggcaacggcg gtttgcgcct catcgatatt gacggcatgc
   108301 cctttcggat acagcagcgc gagcttgtcg atttcctgac gtgccgccaa cagattgcct
   108361 tccacgcgct cggcaaacag tgccaatgcg tctgcctcga tacccaaacc gattttgtcc
   108421 agccgtccgc gtatccattg gggcaaagcc gccgcaccga ccggtttggc ttcccacact
   108481 tccccctttg ccgccaatgc ggcaaaccat ttggactgga gccgggtttt ctccagtttg
   108541 ggcagcaaaa ccagcgttac cgtatcttcc ggcagtcggg cggcaaaatc ctgcaatgcc
   108601 tcgccgccgt ttttgccggg ttttccgttg gggatatgca attccaacag tttcaaatcg
   108661 gcaaacagac ccgcactgcc tgcggtttgc agcagttcgt tccaatcgaa agacgcgtcg
   108721 gcggtatagg cttcgcggtt gagataaccc tgcttcttcg ccgccgccct caatgcgtcc
   108781 actgcctcga tacgcagcag ttcttcctcg ccgtggatga cgtacagagg tttcaaaggc
   108841 gcgtccgtat cgatgcgtcc gatatgtgcc gccacgtcat tccgccttca gaaaggtcag
   108901 gcggcggaca atctgttcgg caacatcctg ccgcatttcc gcccacaggg tttcttcttc
   108961 ttcctgtttg cccaaaattt cgttgtcggc ataatccaaa atgcggcgga cggacacggt
   109021 catcggtttg ccgaccggct cgccgcgttt caatacctgc gcttcaaccg tcaatatcaa
   109081 aagatattcg ttgatgactg ccgcacgggt aacggtatag gtttccttgt tttgggaaac
   109141 gctgtctata cgcagggtca tctgcgcgcc ggcagcatcg tccaccctgc ccgaagcctg
   109201 atacagcgcg gtttccaaag gaaactgcaa tgcctgtccg ccttcgatgt gccagctccg
   109261 gtaggtcagc ggcggagaaa tgccgtctgc acctttcagg tggaaaccgc acgcgcccag
   109321 caccaaggct gcggcagtaa ggaatatttt gttcatcgct tgcctttctc aaaagcataa
   109381 agtcggtaga ttatatcagc ggcgtgttca gacggcattg ccgtccagcc aaatcaggct
   109441 cgccatacgc cccgtcgctc cgtcgcggcg gtaggaaaag aaaatatccc gttccaaaac
   109501 cgtacaatgc gtgccgccgt acacgccgcc cacgccttcg cgcctcagaa tcaggcgggc
   109561 gagcgcgtag aggtcggcaa ggaatttgcc gccgccgatg ggttcaaatg cagcagcggc
   109621 ttcgggcatg gacgtgcaga acgcatcaaa cacgtcctgc ccgacttcaa acgcatccgc
   109681 actgatggca gggccgagat acgccataat ttcgacaggc ggaaccttca tcgcggcaat
   109741 ggtgttttgc aatacgccgc ccgccaaacc gctccagccc gcgtgtgcgg cggcaaccgc
   109801 cgtacctgcc ctgtcgcaaa atagaacggg cagacagtct gcagtcatca cggcacagac
   109861 ggctttaccc gtgtcgtcca cggaagcgtc ggcatcgggc gtgccgtcca atgcttcggc
   109921 agcattgacg acaatggtgc tgtggatttg attgagatag gcaacgggca atccgacctg
   109981 ctgttgcacg atttcgcggt tgcggcgcac ggtttcaggg tcgtcgccga cgtgcgtacc
   110041 gaggttcaaa ctctgatacg caccttggct cacgccgccg ttgcgcgtgg taatcagggt
   110101 tttcacattg gcgggcgcgg gccaatcggc ggtcaggaag tttttgcctt tcggggcgag
   110161 atttagggtt tctgtgatgg ttttcattgg atattggaat aggtgtgcgg cggaaagggt
   110221 tgattaactg ccttcggggc aaatggatgc ttgttttttc gtcattcccg cacaggcggg
   110281 aattcggaga ttcgggattg ttttcaaacg ttcgggtcgg attcttgccg gtgcgggaat
   110341 gatgccctta tatactttct ccgagcttta tatgcttcaa caggggacgg cacatcaagc
   110401 accgcactgc gtgttgcccg aacgaggctg ccctccgatt agattctatc gctataaaca
   110461 gacgggtttc aaccgaaaag gaatggggat aaagtccatt tccgacacct ctcgggcgat
   110521 gccgtctgaa aaccaatctc cactttcaga cggcattgtt taggcgcggt cattttgcaa
   110581 attaaggtta aggcgtgtat gcttgccttc ccttaaaaac aaacacgcaa cgcattatta
   110641 tgaatatcct cagtaaaatc agcagcttta tcggaaaaac attttccctc tgaacctcgc
   110701 tctttgccgc cgccgctttt ttcgcgcccg acaccttcaa atgggcgggg ccttatattc
   110761 cttggctgtt gggcattatt atgttcggta tgggtttgac gctcaaacct tccgacttcg
   110821 atattttgtt caaacacccc aaagccgtca tcatcggcgt aatcgcacaa ttcgccatta
   110881 tgccggcaac cgcctggctg ccgtccaaac tgttgaacct gcctgccgaa atcgcggtcg
   110941 gcgtgatttt ggtcggctgc tgcccgggcg gtacggcttc caatgtgatg acctatctgg
   111001 cgcgcggcaa tgtggctttg tcggttgccg ttacgtctgt ttccaccctg atttccccat
   111061 tgctgactcc cgccatcttc ctgatgcttg ccggtgaaat gctggaaatc caagcggccg
   111121 gtatgttgat gtccatcgtc aaaatggttt tgctccccat tgttttgggt ttgattgtcc
   111181 ataaggtttt gggcagtaaa accgaaaagc cgaccgatgc gctgccgctg gtttccgttg
   111241 ccgccatcgt gctgattatc ggcgcggtag tcggggcaag caaaggcaag attatggaaa
   111301 gcggcctgct gatttttgcg gttgtcgtac tccgcaacgg catcggccac ctgctcggct
   111361 tctttgccgc caaatggacc ggcctgcctt atgatgcaca aaaaacgctg gccatcgaag
   111421 tcggtatgca aaactcgggc ctggccgccg cgcttgccgc cgcacacttt gccgccgcgc
   111481 cggtcgttgc cgttccgggc gcattgttca gcgtgtggca caatatctcc ggctcgctgc
   111541 tggcaactta ttgggcggcc aaagccggta aacataaaaa accctaaacg cctttcatca
   111601 taatgccgtc tgaacggggc aaccgaaacc ggcggtttct gcccggcccc ttcagacggc
   111661 ctttgtttga aatcggcggg gacgattgcc cggctgctcc ggttcggctg cctgcgttcg
   111721 ccccgccttt caagccgcct cactccctta cataaaccac ttcgacatcg tagtcgtctt
   111781 cgttccaatc gtcatcgtcg tccgcgccga atttgtcctg ccattcctct tcattgctca
   111841 aagacgaatc caaaccggct tcaagacgga ggacggacaa caaatgatat atgtcgtccg
   111901 gaatcggtgc ctcaaacgaa acggtttcgc cgctttccgg atgggtaaaa ctcaagcggt
   111961 aggcgtgcaa cgcctgacgc gcgcccaaac ttttaacggc ttctttcacc gtgtcgccgc
   112021 acggatggcg cgggttgccg taaaccgggt cgccggcaag cggatggttg gcctcgcgca
   112081 tatggacgcg gatttggtgc gtcctgcccg ttccgagcga gcattcgatg tagctgtggg
   112141 caagatagcg ttccaacact ttgacgtggg ttacggcagg cttgccgccg aatttgacgg
   112201 ctgccatttt caggcggttg tgcggatcgc gtccgatttg ggtttcgatt ttaccgtcaa
   112261 aggggacgat gccgttggcg acggcgcggt agatgcgttt gaccgtgcgt tcttgaagct
   112321 gcctcacgag ggaattttgc gccggcaggg ttttggcaac caccatcagc ccgctggttt
   112381 ctttgtccaa acggtgtacg atgcccgcgc gcggaatttg ggacagctcg gggcaatgcg
   112441 ccaacaggcc gttgagcagc gtccccgtcc agttgcccgc cgccggatgc accaccagtc
   112501 cggtcggttt gttgacgacg atgacggtat cgtcttcgta aacaatatcc aaatccatag
   112561 actccggaac aaacgccaga ttttcctcac tcggacggac ggttacacaa atttgctcgc
   112621 cgcctatcat tttgtctttg ggttgcgaag gtttatcgtt tacaataacc gcgccttctt
   112681 taatccatga tgtcaggcgg ctgcgcgaat agtcgggcag aagtttcgcc aacaccgcat
   112741 ccaaccgccc gccggcaagc tcgagcggaa cggtcagacc gatacggttt tcagttccgg
   112801 aagctgacgc aaagtctgaa tcgtcgctat aatcggattc attatcaaag gaagtattct
   112861 gcatgaaaaa aattctttta acggtttcat taggtttggc actgagtgcc tgtgccactc
   112921 aaggtacggc cgataaagat gcccagatta cccaagattg gagtgtggag aagctctatg
   112981 ccgaagccca ggacgaattg aacagcagca attatacgcg ggctgtcaag ttatacgaaa
   113041 tcttggaatc gcgcttcccc accagccgcc atgcccggca atcccaactg gataccgcat
   113101 acgcctatta taaagacgat gaaaaagaca aggctctggc ggcaatcgaa cgcttccgcc
   113161 gcctccatcc gcagcatccg aacatggatt acgcgctgta cctgcgcggc ttggtgctgt
   113221 ttaacgaaga tcagtccttc ctgaacaaac tggcctcgca agactggtcc gaccgcgacc
   113281 cgaaagccaa ccgcgaagcg taccaggcgt ttgcggaact cgtccaacgc ttcccgaaca
   113341 gcaaatacgc cgccgatgca accgcacgca tggtcaaact ggtcgatgct ttgggcggca
   113401 atgaaatgtc ggtggcgcgt tactacatga aacgcggcgc atatatcgcc gccgccaacc
   113461 gcgccaaaaa aattatcggc agctatcaaa atacacgcta tgtcgaagaa tcactggcca
   113521 tattggaact tgcctacaag aagttggaca agccccagct tgccgccgat acgcgccgcg
   113581 tattggaaac caacttcccg aaaagcccgt ttttgacgca cgcttggcag cccgacgata
   113641 tgccctggtg gcgttactgg cattaagccc atcctcgaaa tgccgtctga agccctttca
   113701 gacggcattt tccgttccgg caccagcctt cccgttcact caaacggttt tttctgccaa
   113761 tagaatttat acactctcaa acgttgagcc tttacgcctc cgcgtcccaa gccgaattgc
   113821 agcgcgcccg acaaatccgt ccgcaacaac ttgatgccgt gtgcgcggac acggttctgc
   113881 accgcttcgg tcggatgctt gtaggcgttg gcataaccgc ttgaagcaac ggcatattcg
   113941 ggcgaaacgg cattgaggaa cacgcccgac gaggacgtat tgctgccgtg atgccccaac
   114001 accaacacct ggctgtacag gttgcctcca tacttgccga ccaggctttc ctcgcccttc
   114061 gtatccaaat cgcccgttac cagcagtgcc gcaccgcccg ccacaacacg caaaacacaa
   114121 cttttcccat tatcatcgat atttttgcgt tcagacggcc tcaaaaactc gaaatccacg
   114181 ccgtcccatt gccaacgctg ttccgcacaa tgccgcgccc cctcataaaa ttccggctgt
   114241 ccggcataaa tcccgccgtt cggtatcttg ccgactgcct gaaagccgcc gtcgtggtcg
   114301 ctgtcgtgat gcgacagaac cagcttgtcc aaacggcgga cacccgccgc attcaaactc
   114361 ggcacgatac ccgtttgtgc cgccgctacc gttcccgtat cgaaaagcag atggcggttt
   114421 gccgtccgca caagcaccga caagccctgc cccgcatccc aaaccgtaac cgcagcttcg
   114481 ttttcaggta ctgcttcagg acggtaggat acaaaccctg ccaacagcaa caccgcccac
   114541 ggacgcaaac ccaagccgcg cggcagcagc aacagcaaag cggcacacac cgccaacacc
   114601 agcagcggca aaggtgcggc ggcaacggca aactcaggcg acacatcggc aagccacacc
   114661 aaaaaccgca aagtgtattc cgccaaaaat gcccccgcct gctgaagcgg cgcaaacggc
   114721 acgaccgaac ccagcaacgc cagcggggtc aacacccaag aaaaccacgg tatcgacacc
   114781 gcattgacca aagggctgac caaaggcagc gaggcaaaca gataaccgag caaaaccaga
   114841 gacaacaccg aagccgccca ctgcccgcgc acggcggttt gccgtttccc ctcgtacaaa
   114901 cgccctgcac acgcccatat cagggccgcc accaaaccga aagacagcca agtaccgaca
   114961 cccaaaaccg ccaaagggtc gaacagcagc accgccgcca acgcctgcca ccacgtcgcc
   115021 cacgccgaca gtcttcccct gcgccacgcc cacgcgaacg ccgccaacat caaaacactg
   115081 cgctgcgtcg gcacggaaaa accggcaagc agcgcgtaaa acagcgcgcc cgcacacccc
   115141 gccgccaaaa cccacgcgcg cggccgggcg ggaaggcgcg gggaacaggc aagcagccgc
   115201 ttcgccagcc acgcaaacag caccgccacc atcgtaacgt gcaaacccga gatactgacc
   115261 aaatgcgtca gccccaacgg ccggaacgcc tgccacaatc cggggcgcaa tgccgactgt
   115321 tcgcccacgc tcaacgcgcg catcagcccg atgccgtctg aaagcccgcc gtccgcatcc
   115381 gcctgccgcc aattgcggct gatgcggctg cgccaaaccg caatccccca accgcttccg
   115441 ccatgcagca aaaccctgtc cgcaccgacc gtccccacgc cgcctacccc gttggataat
   115501 gcccacgcct cacggttcaa accgcgcagg ttcaactccc cgacgacggg gtgcacgcgt
   115561 gccgttatcc gccatctgct cccgaccgcc cattcgcgcc gtttgtagtc cgacagcagc
   115621 aaatcaaacg tccgaccgcc gctgtccaca gcctttgccg caaactgcac gcgccgcccg
   115681 tccgacctcg gcatatccgt cacttccacc gtcaaaggca cgcctgaaac cgcctccgcc
   115741 cgccattgcg aagacagtgc cgcttccgtt ctgaatacgc cgtaagccgc gcccgccaac
   115801 acgcacagca tcagaccggc aaacgcaaaa cgccttgcaa gcacagccaa aaccgcaaaa
   115861 gccgccaacc aaaacggcca atgcggtaca accgacagtg cgaacgaaac tgccacgccg
   115921 acaacccaac acggcaaaaa ccaacgacgg aacacgtctc cccactcccg atataaaaaa
   115981 caaacgatga taaagcaaag ccataaaacc gtcataacaa gatttgaaaa tgataagaat
   116041 gcaaacgaaa agcctcagta ttatccgaga caacggcaaa ctttccatcg tcattcccgc
   116101 aaaagcggga atccaaagac aaaaagccgc agaaatttat cggaaagaac cgaaccacaa
   116161 aaaccggctt cccaccttcg cttgaataac agaacaaaga aagcggaaat aaaaacatca
   116221 ggattctttt ttaaattggc aataaaacac caaaatgaat gagttacaca aaaaaatact
   116281 caacaccacc caaccggcgt aaaatgcaaa acattatcgc tattaaaacg gtaaaacctt
   116341 atgaaagcca aacgttttaa aattaacgcc atatccttat ccatctttct tgcctatgcc
   116401 cttacgccat actcagaagc ggcattggtg agagacgatg tcgattatca aatattccgt
   116461 gactttgcag aaaacaaagg caaatttttt gtcggcgcaa ccgatttatc agtgaaaaac
   116521 aaacaaggtc aaaacatcgg caacgcgtta tccaacgtac cgatgattga ttttagcgtt
   116581 gcagatgtca acaaacgcat agcaacggta gtcgatcccc aatatgccgt cagcgtcaaa
   116641 cacgccaaag cagaagtcca tactttttat tacggccaat acaacggtca taatgatgta
   116701 gccgacaaag aaaatgaata ccgcgttgtc gaacaaaata actatgaacc ccataaagct
   116761 tggagtgcga gcaacttagg ccgcctcgaa gattataata tggcgcgttt taataagttt
   116821 gtaaccgaag tggcaccgat tgcaccaact gatgccggcg gcggattaga tacttacaaa
   116881 gataaaaacc gtttttcctc ttttgtcaga atcggtgcgg gcaggcaatt agtttatgaa
   116941 aaaggggttt atcatcaaga aggaaatgaa aaaggctacg atttgcgcga tctttcacaa
   117001 gcctatcgtt atgccattgc aggtacgcct tataaagata ttaatattga ccaaacaatg
   117061 aataccgaag gtttgatcgg ctttggtaat cataataaac agtattcggc agaagaactt
   117121 aaacaagcac tttcgcaaga tgcgttaaca aattacggcg tgttgggcga tagcggctct
   117181 ccgttatttg cttttgacaa acaaaaaaat caatgggtct ttttgggaac ttacgattat
   117241 tgggcaggtt acggaaaaaa atcatggcaa gaatggaata tctataaaaa agaatttgca
   117301 gataaaatca aacaacacga taacgccggc accgtcaaag gtaatggaga acatcattgg
   117361 aaaaccacgg gtacaaacag ccatatcggt tcgacagcgg taaggcttgc caacaatgaa
   117421 ggagatgcga acaacggaca aaatgttacc tttgaagaca acggcacttt ggtattggat
   117481 caaaacatca accaaggcgc gggcggcctg tttttcaaag gcgattacac agtcaaaggc
   117541 gcaaataatg acatcacttg gttaggtgcg gggattgatg ttgccgacgg caaaaaagtc
   117601 gtttggcaag tcaaaaatcc gaatggcgac agattggcaa aaatcggcaa aggcactttg
   117661 gaaataaacg gcacaggcgt taaccaaggg caattaaagg tcggcgacgg tacggttatt
   117721 ctgaatcaaa aagccgatgc cgacaaaaaa gttcaggctt tctcccaagt cggcattgtc
   117781 agcggacgcg gtacattggt attaaatagt tcaaatcaga ttaatcccga taacctatat
   117841 ttcggtttcc gtggcggtcg tttggatgcc aatggcaatg acttgacttt tgaacacatc
   117901 cgcaacgtgg atgaaggcgc gcgcattgtc aaccacaaca cagaccacgc ctccacaatc
   117961 acactaacgg gtaaatcttt aattaccaat ccgaacagcc tttcggtaca tagcatacaa
   118021 aatgactatg atgaagatga ttatagttat tattaccgac caaggcgacc catcccacaa
   118081 ggcaaagatc tttattacaa aaactatcgt tattacgccc taaaatccgg cggcaacgtg
   118141 aacgcaccga tgcccgagaa cggtgtagca gaaaataacg actgggtctt tatgggatat
   118201 acgcaagaag aagccaagaa aaacgcgatg aaccacaaaa acaatcagcg tattagcggt
   118261 tttagcggtt tctttggcga agaaaacgaa aaaggacata acggggcatt aaaccttaat
   118321 ttcaatggca aaagcgcgca aaaccgtttc ttgttaacag gcggcgcgaa tttaaacgga
   118381 aaaataagcg taactcaagg caatgtcttg ttatcaggtc gtccaacacc acacgcaaga
   118441 gattttgtga acaaatcttc agcccgaaaa gacgcacatt tctccaaaaa caatgaagtc
   118501 gtatttgaag acgactggat aaaccgcaca ttcaaagccg cagaaattgc ggttaaccaa
   118561 tccgcatcat tctcttccgg aagaaatgta tccgacatca ccgccaacat caccgcaaca
   118621 gacaacgcca aagtaaattt gggttacaaa aacggcgatg aggtttgcgt gcgctcggac
   118681 tataccggtt acgttacctg caacacaggc aacttatccg ataaggcttt aaacagcttt
   118741 gatgcgacac ggattaacgg caatgtgaat ttgagtcaga atgcggcatt ggtcttgggc
   118801 aaggctgcat tatgggggca aattcaaggg caaggaaaca gccgtgtcag cctaaaccaa
   118861 catagcaaat ggcatttgac cggcgacagc caagtacaca atctgtcatt ggcggatagc
   118921 catattcatt tgaacaacgc ttccgatgcg caaagtgcaa ataaatacca cacgatcaaa
   118981 atcaatcatt tatccggtaa cgggcatttt cattatctga cggacttggc gaaaaatctt
   119041 ggggataaag tgcttgtgaa ggaatccgca tccggccatt atcagctcca tgttcaaaat
   119101 aaaacaggcg aacctaatca ggaagggctg gatctctttg atgcatcatc cgtacaagac
   119161 cgctcccgcc tttttgtttc cttggcaaat cattacgtcg atttaggcgc attgcgttat
   119221 acaatcaaaa cagaaaacgg tattacccgg ttgtacaatc cttatgccgg gaaccgccgc
   119281 ccagtcaagc cggccccgtc tcctgccgca aacacggctt ctcaagcaca aaaggcaaca
   119341 caaacggacg gtgcacaaat tgccaaacct caagatattg tcgtcgcacc gcccagcccg
   119401 caggcaaatc aagccgaaga agcaaaacgc caacaagcaa aagcggagca agtgaagcgt
   119461 cagcaagcag aagcagggcg taaatcggca gagttgtcgg caaaacaaag ggccggggaa
   119521 gaggaacgcc gtcaaacagc ccaatcccag ccgcaacgcc gcaaacgccg tgccgcaccg
   119581 caggattata tggcagtttc ccaagaccgt ccgaaacgtc gcggacgcag atctactctg
   119641 ccggcaccgc cctcgccatc atttgattca tcagcttacg cagcacccag ggccttgcat
   119701 aatccggact ggtatgaaaa tgattatgaa gaaatcccct tggatgcgtt ggaagatgaa
   119761 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat
   119821 gaaaaagttg aggcagtgag tttgcaacca agagccgcgc agccgcgaac ccaagccgcc
   119881 gcgcaagccg atgcagtcag caccaatact aactcggctt tatctgacgc aatggcaagc
   119941 acgcaatcta tcttgttgga tacaggtgct tcattaacac ggcacattgc acaaaaatca
   120001 cgcgctgatg ccgaaaaaaa cagtgtttgg atgtcaaaca ccggttatgg ccgtgattat
   120061 gcttccgcac aatatcgccg gtttagttcg aaacgcacgc aaacacaaat cggcattgac
   120121 cgcagcttgt ccgaaaatat gcagataggc ggagtattga cttactctga cagtcagcat
   120181 acttttgatc aggcgggcgg caaaaatact tttgtgcaag ccaaccttta tggtaagtat
   120241 tatttaaatg atgcttggta tgtggccggc gatattggtg cgggcagctt gagaagccgg
   120301 ttacaaacgc agcaaaaagc aaactttaac cgaacaagca tccaaaccgg ccttactttg
   120361 ggcaatacgc tgaaaatcaa tcaattcgag attgtcccta gtgcgggtat ccgttacagc
   120421 cgcctgtcat ctgcagatta caagttgggt gacgacagtg ttaaagtaag ttctatggca
   120481 gtgaaaacac taacggccgg actggatttt gcttatcggt ttaaagtcgg caaccttacc
   120541 gtaaaaccct tgttatctgc agcttacttt gccaattatg gcaaaggcgg cgtgaatgtg
   120601 ggcggtaaat ccttcgccta taaagcagat aatcaacagc aatattcagc aggcgccgcg
   120661 ttactgtacc gtaatgttac attaaacgta aatggcagta ttacaaaagg caaacaattg
   120721 gaaaaacaaa aatccggaca aattaaaata cagattcgtt tctaaaatac caaattcata
   120781 gcaaaataaa atgccgtctg aactcaagct tcggacggca tttttatcag actaacaaag
   120841 ctacagctca atgcctttga gtttcgccac ggtattgatg tctttgtcgc cgcgacccga
   120901 caggttgacc aaaatcactt ggtctttacc cattttcggc gcgtttttca ccgcccaagc
   120961 aacggcgtgg ctggattcga gcgcggggat gatgccctcg aatcggcaga gcaagtcaaa
   121021 ggcttcgagt gcttcgtcat ctttggcaac ggtgtattcg acgcgcttga tgtcgtgcag
   121081 atggctgtgt tccgggccga tgccggggta atccaagcct gcggaaacag agtgcgtccc
   121141 caagacttgg ccgttttcgt cctgcatcag atagctgcgg aagccgtgca gtacgccaat
   121201 cggtgcgccc gaagtaatcg gcgcggcgtg gtcaggcgta tccacgccca aaccgcctgc
   121261 ttctacgccg accaaacgta cgttttcttc ttcgatatag ggatagaaca aaccgatggc
   121321 gttcgagccg ccgccgacgc aggcaacggc gacgtcgggc tgtctgccga tagcttcctg
   121381 catctgcgct ttggcttcgt tgccgatgac gcattggaaa tcacgcacca tttcgggata
   121441 cggcgcgggg ccggcggcgg tgccgatgat gtagaacgta tcgtccactc gggcgaccca
   121501 ttcgcgcatc gcttcgttca tcgcgtcttt caaagtacgg ctgccactgt caacgccgat
   121561 gacattcgca ccaagcagct tcatacggaa cacattcggc atttggcgtt gaatatcgtc
   121621 cgcacccatg tacacgtcgc aagtcatgcc gaaacgtgcg gcgacggtgg cagaagccac
   121681 gccgtgctga cccgcgccgg tttcggcgat gacgcgtttt ttgcccatac ggcgggcaag
   121741 caatgcctga ccgatggtgt tgttgacctt gtgcgcgccg gtgtggttca agtcttcgcg
   121801 cttcaaccag atttgcgcgc cgcccaaatg ctcggacaac cgcgcggcgt ggtaaacggg
   121861 gctgggcctg ccgacataat gtttcaaatc gcggcggaac tccgcccaaa attcaggatc
   121921 gtttttcgct tcgttgtagg cctgttccag ctctttcaaa gcgggaatca gggtttcgga
   121981 aacataaagc ccgccgtgtt cgccgaaaaa gcccttttcg tcgggcgcgt ggtagttctt
   122041 catcggattc ttccttatgc ttcaggtttt cagacggcat ttccgttttt tcaaacatgc
   122101 cgtctgaacg ggaaattgtc ggcaattata actgcttttc acacaagccc gccatttcga
   122161 aaaacgcccg atacgccgct gcctttttcg ccaaactcaa aggataggcg cgcgaagtcg
   122221 aaggcaggcg cgtcagtgtc aaatcccgtc cggcaaacgg aaacggcacg gtttcgcccg
   122281 ttttcggcat tttgatgccg ccgccctgaa tatcgagcag gatttccgtc gccttgccgc
   122341 ctgtcgtaca aatatggcgg cagtctggta ttttcgccaa caccgccgcc aaatcgacgg
   122401 tttcaactac ctttaaaaac ttgtcggacg cattgccgtg ttcccttgcc gccttcaaca
   122461 cggtcggaca ggacgcaatc ccccgttcgt gcaaaaacgc cttgattttc tccgcgtcaa
   122521 acgctttttc agagtgcgca gcctccccgt gggacggcat ccggaaatgc gccgcatcgt
   122581 taaaaaacac cagcccgtaa acgcgccaca tatcgttttg gaaattcgga taatgaaact
   122641 gcatcgcgcg tttgtcttcc ttgggcggaa acgtccccat catcatgacc gcagccttcg
   122701 gcggcaacac gggatcgaag ggatgggttt cgatttcaag cagattttca gacacgttca
   122761 atcctccaac aggcaaagcg gcagacagcc ggtttgcacc aaaccgccaa acacggcaag
   122821 ccttcaaaca gcattatcac gccctgccgt tttcggttac aatgccgaca tcaaacggat
   122881 actgtaaaca catttatgtt ccaacacaca ggacgacaca taaagcaccg ccctatgtgt
   122941 cgtcctgatt tggaaggggt tacgccccgc ccaaacagag tctgatcctg ccgccctaaa
   123001 gggcggggtt tcaaccgaag aggaaacacg atgaaagaac acaaagcccg caagcgtttc
   123061 gggcagaatt ttttgcagga cacgcggatt atcggcgata ttgtcaacgc cgtgcgcccg
   123121 caggcggatg atgtcgtgat tgaaatcggc ccgggtttgg cggcaattac cgaacctttg
   123181 gcgaaaaagc tgaaccgcct gcacgttgtc gaaatcgacc gcgacatcgt atgccgtctg
   123241 aaaacgctgc cgtttgcgga taaactggtg attcacgaag gcgacgtgtt gcagtttgat
   123301 ttcaacggca tctcaggcaa aaagaaaatc gtcggcaacc tgccgtataa catctccacg
   123361 ccgcttttgt tcaaactggc ggaggtggcg gacgatgtcg ccgatatgca ctttatgttg
   123421 caaaaagaag tggtcgagcg catggttgcc gcgccgaaaa gcaacgacta cggtcgcttg
   123481 ggtgtgatgc tgcaatattt tttcgatatg gagctgctga ttgacgtgcc gcccgaatcg
   123541 ttcgatcccg cgccgaaaat cgattccgca gtggtgcgca tgattccggt gaaacaccgt
   123601 atcggcaagg cggacgattt cgagcatttc gccaaactcg tgaaactcgc cttccgccaa
   123661 cgccgcaaaa ccatacgcaa caatctgaaa gagcttgccg acgacgatga tttgcaggca
   123721 gtcggcatca gtccgcaaga ccgtgccgaa cacatcgcgc cggagaaata tgtggcgttg
   123781 agcaattatc tggcggacaa ggccgtctga aaaggaacgc ggcgatggaa agcaacctgc
   123841 agaaaaccga gcgtttgatc cgcgaaatca accgcctgca cgcacaatat tcgcaggatt
   123901 attttgaaac cggcaaagtg cggaaaatca atttgagcca cacgctcaaa aacgttccga
   123961 ccgaacatat tttgtcttac cgccttaatt tgcacgaagc agttaacgat tatttagcct
   124021 ttgccgatac gcgcggcata gactttttct accgcgtgaa aaccgcagaa agcatacatg
   124081 ataaaatcaa ccgctatctg gcacgcggca cccaatatcc gaccaacaac atcctgaacg
   124141 atattttcgg cgcgcggctg atttggccgt ctgaaacagt cgccgggatt ttagaaaaac
   124201 ttgacggctg gaaaacagaa tacggcttga aaaactggta tttgcgcgat gccggcggct
   124261 atatcggcat acacatttat ttcaaaaaca gcagcaattt ctactatccg tgggaattgc
   124321 aggtttggga tgaaaaagat gccaaagcca atattgaaaa ccatatggcg tacaaacgga
   124381 atttcgtgcg ttgaattgcc ggcaagccgc cttgaaaccg tttataactg tttataagag
   124441 aataaaccat aaaatacaca aacgtattcc tgacttttcc tttaaccgcc cgagtgaaca
   124501 aactatgaaa tacacagccg cactcctgac ttttctttta actgcctgca tgaacccgaa
   124561 cgatgcgttt ttccaaaacc ggcgttatca aatgccggag gcgcaattga acggttcaaa
   124621 cgccgttttc cattacggtt attcccaaaa tcccgatcat gatttattgg tcgaccaagt
   124681 ccgcgtgccg gacgcgtaca gggcttcatt tgcgcgggat tggatagcga aggagcaagg
   124741 ttaccgcaaa aacgactgcg tactggaaaa agcggaaagc gggggtgtga cgtactacac
   124801 ctgcgatgcg gaaaaaggat tcaacggcac acgtttcgtc atctatgtcg tgaatatgaa
   124861 aaacgacaaa ggctacgtca aagtttaccg gggcatgaat aagccgaatc aggaagagtt
   124921 aaccaaattg gttgccgatt tgaataagtt ttacccttaa tcagccagac ggattcagac
   124981 tgcccgaaaa gaatggacac tatgatcaaa ttcaaaaacg tacacaaaca tttcaaagac
   125041 ctgcacgtca tcaacggcgt aaacttggaa atcaaaaaag gcgaagtggt cgtcgtctgc
   125101 gggccttcgg gcagcggcaa atccacactc atccgcaccg tcaaccagct tgaaagcatc
   125161 gaaagcggcg agatttgggt ggacggcgtc aacgttgccg accccaaaac cgatttgaac
   125221 aaaatccgcg aagaagtcgg ctttgtgttt caaagtttca acctctaccc gcacctgacc
   125281 gtgttggaca acatcaccct cgcaccgatg aaggttaagg ggcaaaatgc ggaacaggcg
   125341 gagaaaaagg caatggagct tttggaacgc gtcggactgg cacacaaaaa agacgccttc
   125401 ccttcccaac tttccggcgg ccagcagcaa cgcgtggcga ttgcgcgcgg gctggcgatg
   125461 gagccgcgcg tgatgctgtt tgacgagccg acttccgcac tcgaccccga aatggtcggc
   125521 gaagtgttga aagtgatgaa agacttggcg gaaagcggca tgacgatgat gtgcgtaacc
   125581 cacgaaatgg ggtttgcccg cgaagttgcc gaccgcgtga ttttcgtcga taaagggcaa
   125641 atccttgaag acgaaacgcc ggaagcgttt ttcaccaacc cgaaacacga acgcgccaag
   125701 cagttcctgc aacaagtcat gacccattga tgctatgccg tctgaaaacc gaacgttcag
   125761 acggcatagc ccgacaccgc aagatgattg agaaagcaca ccatgtattt tgttgaccgc
   125821 accgccgccg tcctcaagcc gaccgcgcgc tttttggaat ggctcaaaag caccgacgaa
   125881 aatatgcccg acctgaccat agaacagctt cgcgccgact gttccgtctt cctcgttccg
   125941 cagttcgacg aaccggaagc cgtcgtttcc tacttcgacg aacgctaccg gcagattttt
   126001 gaggcggagc tggcaggttg ggacatcgac aaggacaaat ggccgcaaga tatggggctg
   126061 aaggcgtttt gggaattttt cgacattgaa atccacgata tggttttgga tatggaagaa
   126121 gcggatttga acatcacgcc cgtgttcgac aatatgatgt aacgccatgc gcgcctttca
   126181 gacggcattg aggccgtccc gcatcctgaa catcctgacc gtatcactcc actcggcttc
   126241 cattgccgtc tgcctgactt ggttttacgg ccggatgatg tggttcggtt tggcggcttt
   126301 ggcggcaagt tacgcatact cgctcaggat aacgaatctg aaacaccgcc atgccataac
   126361 cgccattacc atagaccgcg acggacgggc ggaaatcgta tccggcaaag acaaaacagc
   126421 ggcaacactt gcgggcagca gtatggttac gccttatgcc ttattcttac aatgggacac
   126481 aggcggcaaa accgtccgcc aatgcatcat gcccgacatg acggacaaag aatcctaccg
   126541 gcggctgaaa gtctgggtgc tttggcggca gccgaaaaaa accgccgaaa ccgatacatc
   126601 agactgattt cagacggcat ttgaaaacga aacactcatg aaaacattac aagactggct
   126661 ctcgcatttg gaaaccgccc acagcggcgg tttgatcgac atgggtttgg agcgcgtaag
   126721 cgaagtaaaa aagcgcatga acctgacacc gcaatgcccc gtcgtcgtcg tcgcgggaac
   126781 aaacggcaaa ggttcggtct gcgcctacct gacacagatt tacaaacagg caggcttcaa
   126841 aaccggcacg ctgaccagcc cgcatttatt gcattacaac gaacgcatcg ccatcaacgc
   126901 cgaacccgtt tcggacgata cgattattgc ctctttcgag cgcatcgaag ccgcgcgcgg
   126961 cgaaatatcg ctgacttatt ttgaattcaa tgccttggcg gctgtcgaca ttttcatacg
   127021 cgaacaagtg gatgtaatga tattggaagt cggcttgggc ggacgtttgg acgcggtcaa
   127081 cgcgtttgac ggcgattgcg cggtggttac tagtgtggat ttggaccatc aggcattttt
   127141 gggcgatacg gtcgaacagg tcggctttga aaaggcaggc gtgttccgca gcggcaaacc
   127201 cgccatctgc ggtcaaaacc ccgcgcccgc atcattggtc gcacacgccg aagccatagg
   127261 cgcgaaactg ctgatggtac agcgcgattt cgagtttcac gcgatggaaa acatccaatg
   127321 gaactaccgc ttccggccgc agcattcaga cggtcccgca cgcaaccgca atgccctgcc
   127381 cttccccgca ttgcgcggcg cataccagct ttctaacgcc gcctgcgcgt tgaccgtatt
   127441 ggaatgcctc gacgacagac tgccggtgga cattggtgca atcaaacgcg ggctgctgct
   127501 ggttgaaaat cccggacgct tccaagtcct gcccggccgg ccgctgaccg tattggatgt
   127561 cggacacaac ccccacgccg cccgcgccct gcgccgcaac ctaatcaacc ttgcctacgc
   127621 gcaaaagcgc accgccgtgt tcagtatgct gtccgacaaa gacatagacg gcgtgtcgga
   127681 aaccgttaaa gaccagtttg acgagtggta tatcgcgcct ttggacgtgc cgcgcggtat
   127741 gacggcggat gcactgaaag ccaaattgga acaacaccac atcgaaaaca tacagacatt
   127801 cgccgccgtc cgcgacgcgt accgggcggc agcctcaaaa gcgggcgaag acgacagaat
   127861 cgtcgtattc ggctcatttc ataccgtcgc cgacgtaatg tcggtactgt gaaaaggaaa
   127921 cttctatgtc cgaaaacaaa caaaacgaag tcctgaccgg ttacgaacag ctgaaacggc
   127981 gcaaccgccg ccgcctcgta acggcaagct ccctggttgc cgcctcctgc atcctgctgg
   128041 cagccgcact cagttccgat cctgccgaca gcaatcccgc accgcaggcc ggcgaaaccg
   128101 gcgcaacgga aagccaaacg gcaaacacgg cacaaacccc tgccttgaaa tccgccgccg
   128161 aaaacgggga aaccgccgcc gacaaaccgc aggacttggc aggcgaagac aagccttctg
   128221 ccgccgacag cgaaatcagc gagcctgaaa acgtaggcgc gccgctggtg ctgattaacg
   128281 accggctcga agacagcaac atcaaaggtt tggaagaatc cgagaaactg caacaggcag
   128341 aaaccgccaa aaccgaaccg aagcaggcaa aacaacgcgc tgccgaaaaa gtgtcggcaa
   128401 ctgccgacag tacggatacg gtagcggttg aaaaaccgaa acgcactgcc gaacccaaac
   128461 cgcaaaaagc ggaacgcact gccgaagcca agcccaaagc caaagaaacc aaaaccgccg
   128521 aaaaagttgc cgacaaaccg aaaactgctg ccgaaaaaac caaaccggat acggcaaaat
   128581 ccgacagcgc ggtaaaagaa gcgaaaaaag ccgacaaggc tgaaggcaaa aagacagccg
   128641 aaaaagaccg ttcggacggc aaaaaacacg aaacggcgca aaaaaccgac aaagcggaca
   128701 aaaccaaaac cgccgagaag gaaaaatccg gcaaggcggg caaaaaagcc gccattcagg
   128761 caggttatgc cgaaaaagaa cgcgccttga gcctccagcg caaaatgaag gcggcgggta
   128821 tcgattcgac catcaccgaa atcatgaccg acaacggcaa agtttaccgc gtcaaatcaa
   128881 gcaactataa aaacgcaagg gatgccgaac gcgatttgaa caaactgcgc gtgcacggca
   128941 tcgccggcca ggtaacgaat gaatagcctc cccattgccg acctcctcgc ctccgccgtc
   129001 atcgccgcct gcatcgtcat ttccacgatg cgcggcgtga ttgcggaagc aggttcgatg
   129061 gtggcatggg tggtttcctt cttttttgcc aaactctttg ccgcaccctt cgccgacctc
   129121 gcctttgcct cgttccaacc ccgcctgttt gcattggctc tgtcattcat ttccctgttc
   129181 gtcattgcct gtctgatcca gaaaatgctc cgttcgctgc tgaccggcgc agtttcggcg
   129241 gtcggtctgg gctttgccaa ccgcattttg ggcggtgtat tcggtgcatt gaaaggcgtt
   129301 ttgattgtta ccctgctgat catgcttgct tcaaaaaccg acctgcccga taccgaagaa
   129361 tggcaacagt cctataccgt accgtttttc gtatcgcttt ccgaagcggt gttaaaccat
   129421 acggacaacg cacccgaatc cctcgacgac gactaatccg catacgtggc gtttcagaca
   129481 gcatacctga tttatgccgt ccgaaacgcc ttttctttcc ttgacacacc ccgacagaca
   129541 aagcgcacct gcctgacttt acacaaatat tgtcaacaat tcgggcaaaa agccgccgtt
   129601 tgctttataa ttccgtttca cccttaaacc tcgaatcgga gaagacgata tgtgcggcgt
   129661 attaggtttg gtcagtcatg aacccgtgaa ccagcttctg tacgacggtt tgcagatgtt
   129721 gcagcacagg gggcaggatg cggcgggcat tgcaacggcg gaaggcggta ccttccatat
   129781 gcacaaaggc aaagggatgg tgagcgaagt gttccgcacg cgcaatatgc gcgatttgac
   129841 cggcaacgcc ggcatcgccc acgtccgtta tcccacggcg ggcaacgccg gcagcagcgc
   129901 ggaggcgcag cctttctacg tcagctcgcc tttcggcatc gttttggcgc acaacggcaa
   129961 cctcaccaac actgccgaac tgtatgaaaa cgtgtgtaac aaacacctgc gccacatcaa
   130021 caccggctcc gattccgaag ttttgctcaa cgtattcgcg cacgaattgc gccgcgaagt
   130081 ctctaaaaac gccgacccgc accggctcaa tgccgacaat attttcaacg ccgttgccga
   130141 agtccaccgc ctggtgcgcg gtgcatacgg cgtggttgcc atgattgcgg gctacggtat
   130201 gctcgccttc cgtgatcctt acggcatccg cccgctggca ttgggttcgc aaaccgacag
   130261 cgaaggcagg aaatcctatg ccgtcgcctc cgaatccgtc gccttcaacg cgcttgccta
   130321 cgatttggaa cgcgatatcc ggccgggcga agcggtattt gtcggcttta acggcacaat
   130381 catcgcccga caatgcagcg accgcgccaa actcagcccc tgcctttttg aatatgttta
   130441 ttttgcccgc cccgactccg tgattgacgg cgtgtccgtt taccagtcgc gcttggatat
   130501 gggcgtgtcc ttggcggaaa aaatcaaacg cgagctgccc gtggacggca tcgacgtcgt
   130561 gatgcccatt cccgatacca gccgccccag tgcaatggag cttgccgtcc acctcaacaa
   130621 accctaccgc gagggtttga ttaaaaaccg ctatatcggc cgcaccttta tcatgcccgg
   130681 tcagtcgacg cgcaaaaaat ccgtgcgcca aaaactcagc ccgatggaaa ccgaatttgc
   130741 aggcaaaagc gtgttgctgg tggacgactc catcgtgcgc gggacgacca gccgcgaaat
   130801 cgtcgaaatg gtacgcgcgg cgggcgcgcg caaagtctat atcgcctccg ccgcgcccga
   130861 agtgcgctat cccaatgtgt acggcatcga tatgcccacg cgcgaagagt tgattgccaa
   130921 cgggcgcagc gcggcggaaa tcgccgccga aatcggcgcg gacggcatcg tgtttcaaga
   130981 cttgggcgat ttggaagccg tcgtcaaagc actcaacccg aaaatcgaat ccttcgattc
   131041 atcctgtttc aacggcattt atcagaccgg cgacatcgat gatgcctacc tcgaccgcct
   131101 gtctgccgag aaatccggct gcggcggtct gaaaatccac ccgagcagga tggaacacag
   131161 catcagcatc agcgatgcgg gcgacgaaga ataaggcttc ccaaaccgcg atgccgtctg
   131221 aaatccgaac gttgggtttt cagacggcat ttcccatatc ggcatcacat ttcccgtttc
   131281 atccgcacac gatatgagta ccgaaaccaa aaactacatt acgcccgccg gctggcaggc
   131341 attgaaagac gagctttatc aactggtcaa taaagaacgc cccgaaatcg tccaaatcgt
   131401 caactgggcg gcaggcaacg gcgaccgcag cgaaaacggc gactatcttt acggcaaacg
   131461 ccgtatgcgc gaaatcgacc gccgcatccg tttcctgacc aaacgtttgg aggctgccgt
   131521 cgtcgtcgat cccgaattgc gcgaagcgac cgaccaagta tttttcggcg cgaccgtcgg
   131581 tctgttgcgc ggcgacgggc gcgagcaaac cgtcaaaatc gtcggcgtcg atgaaatcga
   131641 taccgcgcaa aacaaaattt cttggatttc gccgttggcg cgttgcctga ttaaagcgcg
   131701 cgaaggcgac gaagtggttt tgaacacgcc ggaaggccgt gaggaaattg aaatcctttc
   131761 tgtcgaatac atcaaaatcg actgatcggg aatgccgtct gaaacggcac aagcgcaccg
   131821 tttcccaaat aaaggaatac cgaatgagga aaatccgcac caaaatctgc ggcatcacca
   131881 caccggaaga cgcactgtat gccgcccacg ccggcgcaga cgcattggga ctggtttttt
   131941 acccccaaag cccccgcgct atcgacatca ttaaagcaca aaaaatcgcc gccgcactgc
   132001 cgccgtttgt cagcgttgtc gcccttttca tcaacgaaag cgcgcaaaac atccgccgca
   132061 tccttgccga agtgccgata cacatcatcc aattccacgg cgacgaagac gatgcattct
   132121 gccggcagtt cgaccgcccc tatattaaag ccattcgtgt tcagacggca tcagacatcc
   132181 gaaacgccgc cacgcgcttc cccaacgctc aggcactgct gttcgatgcc tatcaccctt
   132241 cggaatacgg cggcaccgga caccgcttcg actggacgct gttggcggaa tattcgggca
   132301 agccgtgggt gcttgccggc gggctgaccc ctgaaaacgt cggcgaagcc gtccgcatca
   132361 ccggagcgga agcggtcgac gtatccggcg gcgtggaagc gtctaaaggc aaaaaagacc
   132421 ccgccaaagt cgccgccttt atcgcaaccg ccaaccgcct atcccgttaa agcaacaaaa
   132481 attgccgccg gaatgactta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt
   132541 gccgtactat ttgtactgtc tgcggctcgc cgccttgtcc tgatttttgt taatccacta
   132601 taatctcaaa aatttatgcc attaaatcaa taatttctga tgaattttga aaacttaatc
   132661 ccgtcattcc cgcgcaggcg ggaacccggt tcattgagct tcagctattt agaataaatt
   132721 ttgaaactct aatcgcgtca ttcccacgaa agtgggaatc cggttttttg agtttcagtc
   132781 atttccgata aattgcctta gcattgaatg tccggattcc cgcctgcgcg ggaatgacgg
   132841 cggaaatctt gtttatattg aatcaaaaaa acctgcacct taatcagttg gcggtttagt
   132901 ccgacttttg gggtgcagat caagcttcca gacggcattt cctttaaaac ttcatttcga
   132961 gcgcgaggct gaagttcctg cccggtgcgg cataccttcc atagttgctg tcgccgccgt
   133021 gccggtttgc cgtgctttcc gcagtctggc gcaaggattc ccaagtaacg tagcggtagt
   133081 tgccgatatt gtagatagcc gccctcaagg tcagccgttt tttcagattc agataagcgg
   133141 aaacatctgc cgttttccaa gaagacgtaa cccttcctgc cgaatatcgt ttttgatcgc
   133201 ctgccagata agcaagctcg tcagggtttt tccctttgga ataggtcagc ataatgtttg
   133261 cgccccattt cccctcgggc tggtcgtatc cgaaccccaa aacataacgc gacggctgta
   133321 ccgcatccaa agcatagctg cggagggaca agtccggccg gttggatacc gatttcggtt
   133381 tgatacggtt gtacgccaat gtggtgtaca ggccttcggg cagtttgccg tatacgccgt
   133441 tccagtcgat tttccccaag atgttgatgc cttgaagcga catattttgg gcattgtaat
   133501 aatcgcgtat atcaatctct gtcaatcgtc ctgctgaatc cggcaatttg gttttttgat
   133561 cggcaacggc aatcatatcg gtataacggt tgcggaaact gctgatttcc aaaaagccga
   133621 aatcgccctt ccactgcaaa ccgatttccc ggttggctgc cttttccgat ttcagggcgg
   133681 gacgctgcca gcctttcgga taatcgtgat aaatgtctat cccgaaaagt tcttggaagg
   133741 aaggcgttct gaagccgctg gaggcgcggt aagacacgga aaaatgccgg ttcggtttga
   133801 acacgatgcc gctgttccac gaacggtcgg cataccgccc gctgcggacg agttcttccg
   133861 acgtggtgaa gtttttccgg tcgtacctgc cgcccaagct gaaatcaaaa tatttgccga
   133921 ttgaaaaacg gtcgttcaaa gaaatgtgga tattgctgcc gttgattttt cttggcacgc
   133981 atttgcggga acgcagggtt tcgatatagc cgcagaccga cccttcgacg acttcgggct
   134041 tacccaaacg gtacttatct tgattctttt catcgaatcc cgtggattcc gaaatccttg
   134101 ccgcattgtg ggaaagctgt tctgggcggg agatcgcttt ggaagcatcg taaccgaagc
   134161 ccaaagtcag atggtgtttc gtccatttgt ttttcagcga tttctcaaac gaggcattca
   134221 aaacattgtg ctgttcgcgg tagtggaaac ggtcgctgct gtcgtaggaa tacggtttgt
   134281 ccgccgacgc gcggcaggat ttgtccacgg caggatacac ggcgcaattc agcttcagcg
   134341 tgttgttgtc ggttgccacg ccctgtttgt caaacgacaa caccgcctta tccgcccagt
   134401 tgtccgagta tttctcattt tcataacggt acagcaaacc catacggcgg cggcggtgat
   134461 gttcgtcaat aaatttggtg cgggaatatt tcaaacctat gcccttgacc aaatttttat
   134521 cgcccttcca ctcttctata ttcggcacaa aatacaagcc gtcgcggaaa tcgtcgccgt
   134581 cgtacacccc gctcttgtct ctaaactttt ccgcctcgtc cgtaccgtaa tactgttttt
   134641 ccgtcatatc gcggatgtcg taacgctgtt tggtatcctc aaacacgccg ccgacataat
   134701 gcctgccgcc gaagcggtag cccagcttgg caagccaaga gccgctgcgg taatccatcg
   134761 gatcgggcaa tatcctgccg ccgcccgtgt aggcttgggc ggacaggttt tcgtggcgcg
   134821 cctgcgcctc ccgcacctgc gcctcttctt cagcacttaa aggctgattt tgttcaatac
   134881 gttcttttac ccagcggttg agctggcggt tcaaatatat cccgtagccc gccaattttg
   134941 ccacgggctt gagtgtattc tcgccctcta ctaagaaaaa tggctctgtt gtcttgcgtt
   135001 taatatcgta tgtctggcgg aacgcgtcca aacggtctat gccgtattcc accccgtccg
   135061 caatatcgcc gtgcgggcgc gtttcccgcc cttggcgttc ggttcggatt agcagccctt
   135121 cccaaccgtc tttgctgaac cccgcgccga gcgacttcat aaattggcgg tttttactgc
   135181 cgtaagcggt ttttgcctgt atcccccggc ttttgccgtc tgaaatcagg tctgccgcct
   135241 ctttggtgcg gaaggcgacc gcgccgccga gtgcgccgct gccgtgatcg gacgaaccgg
   135301 cgcctttgtc gatttccacc gtgctgatgt tttcatattc gatttcgttg attgcgccgc
   135361 tgccgccgcg tccgccgtat ccgctcaacg atccctgcac ggtaaacgcc tgtatttggg
   135421 caacgccgtc aaccgaaacc gccacacggt ttttatctac gccgcgtatc gagtagccgc
   135481 cgctcgcgcc gttgccctgt tcgacaaccg ccacgcccgg atcgtagcgc gtcaggtcgc
   135541 ggataccgag tacctgttct ttgttcaacg tttccgacgt tttgacgatt ttgcccaaac
   135601 ctgtcgcctc tttcgatcgc cgtcccactt tggcggcacg gacggtaacc tctttcaatg
   135661 attgactttg cgccgcatca agtgccgccg cccccgcttg ggcagcataa gccggaaaag
   135721 cggttgcaat ggccaacgcc gtcaaagtca gcggaaaacc gtgtttctta ttcatttttc
   135781 cacctcttta ttatctttct tcgcaccgaa taccacgccg aattggggtt taacttcagg
   135841 ttctaactgt tcgccaacat cagcttcatt ttcaacttca ttttcaatat cttcagttat
   135901 accaagagat ttcccatcct tattgaaaat agtgccgccc aattccgccg cctgcgggcc
   135961 gtaaaagccg cccgttacaa gaagattgtc ggctttgaaa ctttgaggat tagtcgaacc
   136021 atttcccgaa agattgatgc cgttatcccg agtgcgtgct gttgcgtgga aaccgttgcc
   136081 ctcaatcacg ccgttttcaa tacggaaagc aggttctaca ccgttttgct ccgtcagcgt
   136141 tccggaaatc gatttattgc cgaaatcaac gtcaaattct gcttttgccg ctttatccgc
   136201 cttattatcc cattgaatgg gttcgccgat acgcgcttcc caagtgccgg tataatgcgc
   136261 cgttccggtt tttggaatgt cggcttccgc cgtgcggata cctttcagga aaaggtcgat
   136321 gtccctgcct ttaagggctt ccggggcggg cgggatgccg tctgaaacac cgttgccttc
   136381 ttccggcgat tcttcttcgg gttcttcctc ttcagcttct tcttcgattt cttcagcttc
   136441 atcagcttct tcttcgacga tttcgtcttc gtcttcgccg ttatcatctt cggaaacttc
   136501 gtcttcgctt tcttcggcga tttcttcgtc ttcgctttct tcaccgttat taatacccga
   136561 atcctcttcg tcctgcgcct tcggtttgac ggcggggcgt tcggttttta tccgtccgag
   136621 tttcacatag gtcaaaaagt cgcaacaagc actgacggtc attttcctgc cgtcggcaag
   136681 atcgatggtt ttctctttgc taaccaaagg aatttcacgc ccttcgacaa gaagtttgtc
   136741 aggatgacca aaatcgggca tagtggaaac ctcaaacgga cggggatttt catcagtcgc
   136801 ctcgtcaacg gaaattttta gagaatccaa gattttggtg tgttttccaa acggcatagc
   136861 aggatttgtg cctgatgcgt ttgttttttg tttgcctgcg aatacgccga atacgccgtt
   136921 gtcgttactg ataaaccgtc cggcaagctc ttctccgtta tcgccgaaaa aaccgccctc
   136981 aagccgctga tcggcatcgg tatggaaaaa caaatattct ttatcagcgt gtctcgtctt
   137041 cacctcggtg ttaactttgg cactgccggt aaagcggttg ccgtttaaat cggcagtgat
   137101 gttgtaaatg gtcagcggtt ttttaggttc ctttggatca tttctctttt gcacatactg
   137161 atttttaatc agctcgccct ttaggatttt tttgtcgaaa tcaaccgtat attcggcagg
   137221 atgtttttcc ctgtcgtcgg catccttagc cgcataagaa gttgcaccaa tatcgttacc
   137281 ataaaatgtt atataaccca aatcgtcact ggcaaccgcc ttacctgtcc gatgacgttt
   137341 ggcatcggtc atatattgcc agttgccgaa atattttacc gttcccgcgc tcggtaaaga
   137401 ttgggaagga cgttctccgg aataatatac aaaaccgtca taaccaaaac ggtgggtaaa
   137461 ccgcttacca cctgagttct gttcgatttc atcttttccg tttttagtat atacataacc
   137521 ggcatccaca aatttataat tatatttttt attttccttt tctgacgtcc taatttctac
   137581 atcggaatca cgtttatgaa tttcgctttt aagttgttga agtttatttc cttttgaacc
   137641 gtataaaaac agaacatcac cttctttaaa cgacagcttt tcttctgctt gtttgctatc
   137701 gggaattgcc gtgccatctt cacgatgaaa agcagtattc cgcctcaaca accgcattgc
   137761 cgcaccgacg gcgggccggt tg
//
LOCUS       DAWTCI010000002        79297 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWTCI010000002 DAWTCI010000000
VERSION     DAWTCI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 79297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79297
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.002"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(305..1768)
                     /gene="guaB"
                     /locus_tag="WM481_000148"
     CDS             complement(305..1768)
                     /gene="guaB"
                     /locus_tag="WM481_000148"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFA9079569.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTDERVKALVEAGADVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVTVGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(1915..4473)
                     /gene="glnD"
                     /locus_tag="WM481_000149"
     CDS             complement(1915..4473)
                     /gene="glnD"
                     /locus_tag="WM481_000149"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFA9079570.1"
                     /translation="MPENLSSALETFKQQRNAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEHFQNSALCLMAVGGFGRGEPYPCSDVDLAVVSPAPLSDGIQEQIARFIQTLW
                     DCKLMPSVKSGSVDELCESVRDDITGDTAFLEARFLFGNRQTADELAEKMNVQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLAANLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIRLHLNAKRAEDRLLFDLQPQVAESMGYQDENRRR
                     QSEELMRVFYRAVKTVKQLGGILTPMLRSRVSSTPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRRRFAGF
                     FRSGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAVQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSAVAQKEDIQDPGVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRCLAGNDGNPHALFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFEPPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSCNRRISRRSRYMPIAPSITITPEEDYPDRYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERVEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            complement(4522..4842)
                     /locus_tag="WM481_000150"
     CDS             complement(4522..4842)
                     /locus_tag="WM481_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9079571.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQVPS
                     ESGI"
     gene            complement(5170..5643)
                     /gene="bfr"
                     /locus_tag="WM481_000151"
     CDS             complement(5170..5643)
                     /gene="bfr"
                     /locus_tag="WM481_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9079572.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELAKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(5671..6135)
                     /gene="bfr"
                     /locus_tag="WM481_000152"
     CDS             complement(5671..6135)
                     /gene="bfr"
                     /locus_tag="WM481_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFA9079573.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(6610..7593)
                     /gene="lipA"
                     /locus_tag="WM481_000153"
     CDS             complement(6610..7593)
                     /gene="lipA"
                     /locus_tag="WM481_000153"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFA9079574.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFTNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(7586..8209)
                     /gene="lipB"
                     /locus_tag="WM481_000154"
     CDS             complement(7586..8209)
                     /gene="lipB"
                     /locus_tag="WM481_000154"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFA9079575.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGIYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTQ
                     INPCGYAGMEMTQIADFVQPCPAPDEVASKLTAHLETQLTPKADNNE"
     gene            complement(8211..8486)
                     /locus_tag="WM481_000155"
     CDS             complement(8211..8486)
                     /locus_tag="WM481_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFA9079576.1"
                     /translation="MTEQKNKASLIEFPCAFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            8671..9741
                     /locus_tag="WM481_000156"
     CDS             8671..9741
                     /locus_tag="WM481_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFA9079577.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVYALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FIQNTLLPWLKNTIGGYVEIDQASIIAWFQAHTGELSNALKAWFPVLMKQGGNIVSSI
                     GNLLLPPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLMLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGELMGFVGMLAGLPLAA
                     VTLVLLREGAQKYFAGSFYRGR"
     gene            complement(9848..10795)
                     /locus_tag="WM481_000157"
     CDS             complement(9848..10795)
                     /locus_tag="WM481_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HFA9079578.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(10810..11217)
                     /locus_tag="WM481_000158"
     CDS             complement(10810..11217)
                     /locus_tag="WM481_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFA9079579.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDTGKYAEILRYTGGNRYEVFYRGT
                     HWQAQNTGQEVFEPGTRALIVRKEGNLLIIANP"
     gene            11298..11957
                     /gene="ung"
                     /locus_tag="WM481_000159"
     CDS             11298..11957
                     /gene="ung"
                     /locus_tag="WM481_000159"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFA9079580.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPEADVFNA
                     FRLTAFDRVKVVILGQDPYHGVGQAHGLAFSVRQGVRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGILLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSQHGIEPINWK
                     L"
     gene            complement(11994..12329)
                     /locus_tag="WM481_000160"
     CDS             complement(11994..12329)
                     /locus_tag="WM481_000160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079581.1"
                     /translation="MPNLVTAVLAGRFLRWRIPYPTHQTDNISSRILTARICWYESSA
                     QAEKAVAPKTVARINCLNFMMLLLLHKAFGIVKPAAYGFAAVAALQAVLQETLKHDHC
                     IKSCSNSAK"
     gene            12752..13639
                     /locus_tag="WM481_000161"
     CDS             12752..13639
                     /locus_tag="WM481_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFA9079582.1"
                     /translation="MSAPVILSAAATKGGVGKTTLIANVSAVLADIGLRVLMIDCDVQ
                     PSLSKYYPISHRAPNGIVELLLGENTEEIIRSTISNTVFPNLDIVISNNISAFVETEV
                     TSRPDRAFLLKSKLHSQYIQNNYDVVLIDTQGAVGPLLDTAAFAATCLLCPIMPEVLS
                     AREFLTGTQELLKRLQKGEAMRIPMPQLRAIIYAQDRTNDAKFIASSIKDFFSNTLDD
                     RRKLLSVAVPAAKAYKEATTLRVPVHCHERKQLGKMLPAYEIMHRIVYEIFPGIEQKN
                     LRGSCFNDMASLLQKEVQS"
     gene            13636..15126
                     /locus_tag="WM481_000162"
     CDS             13636..15126
                     /locus_tag="WM481_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFA9079583.1"
                     /translation="MNLDQNKATLSLHIGTPAQTNMSFGQEDTPSRGMLMSLPISEID
                     FFDKNPRRLHDVSSYAAIKESIRSSGIQQPVHVTQRPGESKYVLAQGGNTRLKILCEL
                     LEETGDNRFRMMPCIYIPYSDETTLQVAHLVENELRAEMCFWDKACKYAEIRDIFQDE
                     SDSGKLSLRELEVMFSDKGLTVSHKTLGLFFFASDNLQELQEYAFKLSHAKVEEIKKL
                     HSTLFSISKSIQLDDSFQYLWSDVLEQWHNQYSATELDVHELYQFVLDRFKFEYGSDA
                     FSETENSSDKDKIETAAKQDVNLHQKDSDMEFVPAKVNGTKADAEDEPKEAEPITLEQ
                     TSKKLFSAVRKLLAKVSLSDCFKIHKDFPYGWYVEYPAFEHISASSAGNAIYAIDVLH
                     EEAGNVFTFLWKLSGIDTFMFDVGDSSNPLLTLKSDSKLRIAYEDPDKYDEYTNCGIG
                     NKENIIASLLNWQTNTSHEFHANIVEILSLSTQYNNLRKTGAKSEE"
     gene            15116..15721
                     /locus_tag="WM481_000163"
     CDS             15116..15721
                     /locus_tag="WM481_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079584.1"
                     /translation="MRSKPLISNPIYRLFNTDPVYTNLALTRLASAPNTPRESSYNAL
                     VAREHEDVLNTLKRMNPKRIEKLASMCPDVVTYSIDLKVLKSKISEMEHYEQRFMEQM
                     QRLKWMLEHKASNLMIMNLCSLISTAEIKKLRIEMNIPVVKGRIVMPSIDVRVRVLKA
                     WSKSEKEPDLFIRYQLLIDEFPDYSLAQLHSIINDVKFFGV"
     gene            15718..16473
                     /locus_tag="WM481_000164"
     CDS             15718..16473
                     /locus_tag="WM481_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079585.1"
                     /translation="MSMYMDFSTSYDYETGFVPSKNMSTDNFIRSLVALWEEPATYVR
                     QFAQAVGSVSEGYFLSWFVEKCLPGEWVSLNNDLLFNEAGITAKQWRNIRNSLLQKGI
                     LLNRRTVAPTESQFTLNDALFEDLVSKHGKSDVLDMVSPPISINKLHLKTLADRGLAF
                     VSILYLSFLQSEAEYQDLSKRGDWSPWTHAAEKHVFYSTMLSRRQQERAIAELTSFGL
                     IESCVDGMPAARRCRYSLKMLADLTSEYLNGCV"
     gene            16473..17990
                     /locus_tag="WM481_000165"
     CDS             16473..17990
                     /locus_tag="WM481_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079586.1"
                     /translation="MVTATKLPPPKVFNSELTQCWQALQGGRRVAIYRCLIDITGSLK
                     TAAMLSQLIYWTRVSKEVAERDGWIFKSIAQMEAETGLTVREQRTCKNKLLETNLIQT
                     CRKGVGAALAIKVNLDAIAELIAKSSGTDDQLALTLADLQNTSSLYFRKHFSKRIAYH
                     RDLVSITGCINSAVLLSCVLNDAVGLVSQNPHLQRHAFATLTAADWEERTSLSYKSQL
                     TARNRLKNLNLIYERNFLASRRIFTLVNGHDIVISIKKLLAGKQDDGFSPYNSKKREI
                     LSLAERAKCDWRKGPNGLDKGFQSGFEGDAPLFPSMTSGSVEREYSDVSNWNFKKGRS
                     GKFISVDWGSSELPNRQIIYYINYKGVYNYNYMTVEPNKPRSSEQDHVVVVDDVDLIY
                     PKLFNVQMRQQADLIFTKHLSKSSWALRQEILDEIQGQKKEVTSPLGLLINLCRCASQ
                     GNFTALVAPQVRFQREQDQKLQAYLEKQNNQKQDSLDAMDVELARNNLRKFYSKR"
     gene            18128..19231
                     /locus_tag="WM481_000166"
     CDS             18128..19231
                     /locus_tag="WM481_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1845 family protein"
                     /protein_id="HFA9079587.1"
                     /translation="MQQQCFMKLFLITYKDLKMEFAKQRYIIKPGAIHVAQQFDLKVT
                     TKQSPFSDGYDMARAALALGDKVNQKLTEDDEQFQQWEEFKAHELLLKQHLQKETGRK
                     HHLVWGEYEILSSENDRFKNIGSLTSSGDDGFFMHTKQGIRMWEGNNNKKNGPRWPGI
                     RYGLTLSNELFGMAMSDNPYAQSRLLQIEKQMSEIEKFFETVKKQVHAQIDSLAAAGM
                     KITLIQNNNPVHIRLNVLRAYGFKLVKLLRDYDSFVCAVKTLNIKGMMANKQCNDTLY
                     NGGRMMRKLLNDLYIAVMETRAIKSIRRDSLLDPEQLSKLKSAVEQEILPRIPLSVWV
                     YESQPSLVYIQRKMNQEDLNKLVDIVRDNGLSG"
     gene            19318..19872
                     /locus_tag="WM481_000167"
     CDS             19318..19872
                     /locus_tag="WM481_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079588.1"
                     /translation="MNTMNTQISGMKKYLKFWEYKRLLRVRGIEDLEKEIKLKLVDFE
                     LLIDEAQSFHEACQGLNLIYPIVREHLKLSNKSLAGLDLKKYYIPNMIFFKNVVSSSG
                     RMSRKKFFKWADISTALDDTNASLDERLLHMKVYFDCRQYFCRGRQIAGDLAELFSMK
                     EIFDEILRIENLDRKNLPSNIMVK"
     gene            20252..20683
                     /locus_tag="WM481_000168"
     CDS             20252..20683
                     /locus_tag="WM481_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFA9079589.1"
                     /translation="MSVQLFVRGNLGAEFNLRPVASPKNDEASIVLNFSVASPNFKRN
                     AEGKSEVVSTEWISCEYWNRDAAHLHKILQVGMPVFVTGEERYESYTNRDGIEVRVRK
                     LRVQDVFIVPTERVEAIKLRPKREQSQDNNHVEDADDDIPL"
     gene            20875..22914
                     /locus_tag="WM481_000169"
     CDS             20875..22914
                     /locus_tag="WM481_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFA9079590.1"
                     /translation="MRLFLCEKPSQAKDIGKVLGVLGGRHDGYFQNGDTIVTWAFGHI
                     LTQAAPDAYGEQYADFGNISALPILPEVWQMQVPPKVSKQFKVIKSLLAKADEVVIAT
                     DADREGEVIAREILDYCGYRKKISRFWTSGLDTQSVKKALSSIMPGKDKENLYQAGLA
                     RGRADWLVGMNLSRAYTAAYSAGYGREHILSIGRIQTPTLKLVVQRDKEIDTFVPYNY
                     YTLSVDFETADKSVFTTLWQVPENWRNTDGYCTNLEAVKALVKQLEKSTGKVITSNKE
                     RKSTPPPLPYSLSALQKEAGKKLGLSASKVLKIAQTLYEKYKITSYPRTPCRYLPKSQ
                     MSEVKDVFAAIQTIDKSMLSVIEKADPERQSRVWNDNQVNKHSHHAIIPTKASNFDLS
                     VLDKNELTVYRMIRDRYIAQFYPDFEYDSTVVEVEACSHLFKASSQSPVISGWKVLLG
                     KDVFEGDQIDEGPALPHLKVNDEVDTLSFNPETKKTTPPPRFTEASLLDEMQTLKDFL
                     KNVEDEQIRKILKSTEGLGTEATRATIIDRLFEMGYMEKKRSKIYATEKGRNLIARIP
                     TMIADPITTAKWELALAGIEAGKLTLAEFMAYQQKVITELVGQAKKDAVGKARPPKQT
                     DSAGTKKQAVARNEDDVCPTCKEGRLRQRGFKENPDKRYWGCSRFPECKHFEWVK"
     gene            22929..23468
                     /locus_tag="WM481_000170"
     CDS             22929..23468
                     /locus_tag="WM481_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079591.1"
                     /translation="MKKLVKKLSDSIFGNTCIEYVLDENHNYKEQYNANKDLFIYING
                     IQVGSISPGELQELQYRVFYSSSLWWSIFKSILGFLLKPAIIALTALPLGVLLLLWLN
                     TDNPEAFHRLFMMLQDPAHFAKAISGLFRLFYCISFFGTFVKLVFNQDSIFSIFNKEL
                     SKNIQSNKTCSMDIVAVRD"
     gene            23572..24144
                     /locus_tag="WM481_000171"
     CDS             23572..24144
                     /locus_tag="WM481_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFA9079592.1"
                     /translation="MDRRNFLKKFVGVGALYTTGAAGLLLPKDVVAATSMADFWSRDR
                     TINCKRADTGEKHEIRFFQQQNGYDLDAYRNACWLMRDAKDGNAMVQIDVGLLNLMYA
                     MQEWARQSGRSNPVITINSAYRTPRRNATIEGAARNSLHMRGKAVDFTMRGVGIGELE
                     QMAKYYNVGGIGIYNSFVHLDTGRVRHWRG"
     gene            24408..24857
                     /locus_tag="WM481_000172"
     CDS             24408..24857
                     /locus_tag="WM481_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HFA9079593.1"
                     /translation="MTQSNQNYFNLTADGIGFLNRPREVQGKRKSSNYYACTIQASRG
                     DNGEKTRFDLRIVGGQAKELFARLLEEFPQLLSKNYRERPTVVVGFRAGDIQPLKFET
                     NNRETGEVLETLSIDGRLLKFKFIKVDGDMWYQSSSQDETQNNPDAS"
     gene            26042..26557
                     /locus_tag="WM481_000173"
     CDS             26042..26557
                     /locus_tag="WM481_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3275 family protein"
                     /protein_id="HFA9079594.1"
                     /translation="MSINLSGTLTVQVIQGRRGPFKTAKLATSIGTFDVKKPILKQFE
                     PGEYQGNFIIEQLKVKAYEWGGGATSYLDAALDWQHLAEFAQEFSSGSDSPQDDPSDP
                     FDPVPENGIRLPEYAETADQVGEMIAQNREHISLGEYILSDRDELNIARSMMKEAGYS
                     YRPQSQTWCLQ"
     gene            26567..27148
                     /locus_tag="WM481_000174"
     CDS             26567..27148
                     /locus_tag="WM481_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079595.1"
                     /translation="MLYLTSRNDLMADAFFIWNRQLMFASLHGRDADMLSFQAQLQVS
                     NERLGFRRPEEVLSCPRLTTAEHCLNLSKYMTKYQTLNYGSVTHMFLYSDILIQPNFD
                     SKTGWVMLDDVTADLDKAAWQLVRQLSDIPLLEHWQNAVLSVLEADKSIMRFTPRIDE
                     EYAVVGVQAVRVDIPEDFDVRLTAMLQSGRLHT"
     gene            27374..27781
                     /locus_tag="WM481_000175"
     CDS             27374..27781
                     /locus_tag="WM481_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079596.1"
                     /translation="MPTLSEKHEVLSGLNRQDAAAIVALIDLKTEDDMEKVLNKLDFM
                     TQNFDARLDAFEKSVDARISSFEKSVDARISSFEKSVDARFSSFENSVDARISSFENS
                     VDARFSAIDTKINAIYWFIGITTTVAVAAAKFL"
     gene            28020..28298
                     /locus_tag="WM481_000176"
     CDS             28020..28298
                     /locus_tag="WM481_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFA9079597.1"
                     /translation="MTATYADCRFEARITSDTRALLKHAAELQGRSMSEFVISAAREA
                     AQKAVADAEILMLSERDQLQFAQALLNPSKPNGALSRALEKHDELFGR"
     gene            28300..28791
                     /locus_tag="WM481_000177"
     CDS             28300..28791
                     /locus_tag="WM481_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9079598.1"
                     /translation="MPLPDFRIRLLDSADDRNAFERPSEPLNRYLQKQVSQDVKRKVA
                     TCFVAVTSDGQILGYYTLAATGVALSNIPAGFRKKLPRYPTVPAVLMGRLAVDGKYGG
                     FGLGSLLLADALKRIGKAEIAAYALIVEAKDEAAIGFYRHFGFTSFEDATKHLFFPLA
                     NLR"
     gene            28887..31370
                     /locus_tag="WM481_000178"
     CDS             28887..31370
                     /locus_tag="WM481_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9079599.1"
                     /translation="MYNPVSKIDELLPDFAGLDQTVTEMQERQNQITTDFNFIIRQVA
                     ETAQKSTEAAVLPEESPETELPDSDIPLTQFLAEFGDSLFNAVTEQNQPVYQGQTYPL
                     LEEVLDNLERPLFAAQREVVRAVLQQLVAEDKPAAIINAEMGTGKTMMSVAAAAAAHQ
                     AGLHRTLVLSPPHLVYKWRREILKTVPNARVWILNGADTLAKLLQIRALGRKPEVPEF
                     FVMGRVRMRMGYHWRPSYAVRRRRIPSEGASEGFAVSEYYCPKCGCEILDEENKPYNT
                     EGGVKNALAKNRRFCKGNHHGKPCKEPLWTLCRKEQSEDGVVSTVYDRVLKTMTTLPG
                     IGPKTAAKIIGMYGEQYLAEILENNIQAFSNLMDENGEFLFDDRQAARIDRDLGKAEF
                     SLGRGGYQPTEFVKRYLPKNFFGLLVVDEGHEYKNYGTAQGQAMGVLARCCNKILCLT
                     GTLMGGYAEDLFFLLWRLWPQMMIEDGFSYNKGNTLGSASMAFMRKHGVLKDIVRHLG
                     TEYSNGAFSSSKAERNSVRTAKAPGFSPLGIMRYVLPITVFLKLRDLGEGVLPGYKEV
                     FRPVEMTEDQQAVYKNMESVLRQHLKVALRNRDNTLTGVVMSALLSWPDCCYKPQDVY
                     WRRQNKILFHTEPQFDEEKISPKEADMLEVVKDSLAKGRKCLVYTVYTDTRDTTGRLK
                     EIFKRHGIKAAVMKATVKADQREDWVEDQLASGVQVVICNPELVKTGLDLLAFPTIYF
                     LQTGYNVYTLMQAARRSWRIGQKEDVEVYFAGYSDTAQQICLELMGQKVAVTQSTSGD
                     MPDSGLDILNQAEDSVEVQLAKRFVDRQD"
     gene            31618..31875
                     /locus_tag="WM481_000179"
     CDS             31618..31875
                     /locus_tag="WM481_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079600.1"
                     /translation="MKFFIFIKPDLPFHKESIESWIKLLEDIGKTALLAMLPLFWLNS
                     YSLTERASGVAVLALVAYFVQVYADKLRKYRDSLNRKDTSC"
     gene            32428..32652
                     /locus_tag="WM481_000180"
     CDS             32428..32652
                     /locus_tag="WM481_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079601.1"
                     /translation="MRYSKNKDYQFFIRQLVSGGEWMFLPKNGRKHSALKHLPTDRKI
                     PIPGSPGQDPRGLLNFKTMVRHIERGGTFD"
     gene            32723..33844
                     /locus_tag="WM481_000181"
     CDS             32723..33844
                     /locus_tag="WM481_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9079602.1"
                     /translation="METQNHLMHPRVAHNHRDNGYFPTDQATLSRIAACLDIPASAGG
                     LLRIFDPCCGTGEALASLASTLQSEKISCQTYGIELEKDRAQEAMSRLTSVIKSDVEN
                     CTMQGSAVGLLFLNPPYGFAAKDNLSNQRTKRLEELFFARTFGTLQPGGVLVMIVPEA
                     ALTEHLTGEIATHCTDVRMIRAAADTYKQLVIFGIRPKNKASISKKQADAQQRLLLDF
                     ENAPETLPAGMPAYCVPIAPNKVFRPRSFKLEQDILERELGQQQSRTLWPSFSQYFGT
                     SAVIAEKRRPLCALGQWHAALALAAGQVNGVVTSTSGRKLLVKGSTHKTKVETKSEEY
                     GKKDRLVVTITSTDRFSPSIRAIDLTEGSSDFGRVLTIK"
     gene            33921..34262
                     /locus_tag="WM481_000182"
     CDS             33921..34262
                     /locus_tag="WM481_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079603.1"
                     /translation="MEQLDSAAPAACDTPSLFDLGRVSMTATVDYRAKEHLPAQLYEN
                     SLYAQILLEAHRHGIWGDIPPEDAKPNQLALKPGEEGRIMSSYKIGDQKIWVVTEWDR
                     SLTTLLFPEDY"
     gene            34498..35079
                     /locus_tag="WM481_000183"
     CDS             34498..35079
                     /locus_tag="WM481_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079604.1"
                     /translation="MQNFIVWMNPDREESAAAAAKLNGAVLTAHTDGVMAAIRRELKR
                     FGLGADLAVFAQTCEEFAKQANADMVCISSSNGDAVAAVTYLEDGYAGKGVSAVADMR
                     YLMPAYRQYFNRPDKMVIFQGALQYLSDEDIAWIFRRHINAIIEWQSNEGLGLYEFGF
                     SLSDVLTDGCRHVLREVVTGWLNREILPELYNV"
     gene            35171..35686
                     /locus_tag="WM481_000184"
     CDS             35171..35686
                     /locus_tag="WM481_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079605.1"
                     /translation="MSIKVDNILLIEGTKESVAEAAKLILFRSPVHPEHWAFDFNRIL
                     PLPENITSDLAKQRIEAWGSSFSPYPTETFVTQREGFIEICFLTGLAPPFGIYRKLAE
                     IFANLDVCFTAKYINQYGSFGGRYEYRNSVLYHCLCEMADIRKFGIAEFDIWYEGLSD
                     RLESDGYLVED"
     gene            36257..37036
                     /locus_tag="WM481_000185"
     CDS             36257..37036
                     /locus_tag="WM481_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079606.1"
                     /translation="MPNWCSNSLHFQGNPQRIAEVAEQILIKTDKGYQFSFQHIVPMP
                     EELILPEVPDSYMQQTWFQIAQQPEKKLINLEREMLEQRDKSFWSVQYRLPDELFSEL
                     KLYFSEKFDWDNLTVGEVFLHFEANSEVASRFRYDEPFAKRIAENIRQYGHPNWYDWR
                     LANWGCKWDVNPDCTFVTVGESGIRISCDTAWGPPEGIYRELAKRFPDVEFEAKYLEE
                     GMWFAGTYEGHEGALFDYPCTDDGVRDFATEHFGCEYDDED"
     gene            37125..37589
                     /locus_tag="WM481_000186"
     CDS             37125..37589
                     /locus_tag="WM481_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079607.1"
                     /translation="MIENQIDKEITQASCEGRFILKQENGKRFLYLNLPEGSDELNTI
                     WQTDEYDFTVPDLEVSIDVESLHTAVRLLNENQGILHGISTKCSAYSFGFEGKLRYER
                     LDVKPFPIKSFSYYLEFYNDWTGTLYELDLSAFLDEFFGECDPESKLDACLK"
     gene            37993..38304
                     /locus_tag="WM481_000187"
     CDS             37993..38304
                     /locus_tag="WM481_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HFA9079608.1"
                     /translation="MKIIFDAVSTMTSKNQTTIPESVRKALGLEKQDKIRFSVLGGGQ
                     VLLEKNNTEEEEFEHDPVVGKFLHFLENSMTKNPNSIKPTSQSRFNRLRQLTWETDMK
                     D"
     gene            38306..38755
                     /locus_tag="WM481_000188"
     CDS             38306..38755
                     /locus_tag="WM481_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HFA9079609.1"
                     /translation="MPDSPMQINGYTIYEHECFTQQLDRLATTIESQKRSEKSSRSEE
                     AKLLERILGEIENIALNPDSPEYRLGKTLGPEYKFWRRAKFMGRYRLFFRYDSNSKII
                     ILAWVNDSGTKRTYGGKNDAYTVFKKMLGNGNPPSDWTALLEVVSKE"
     gene            38870..39940
                     /locus_tag="WM481_000189"
     CDS             38870..39940
                     /locus_tag="WM481_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079610.1"
                     /translation="MSRTNLKGGRKIALWRQTGYIGFYCCLILMMLFFPKDFNRLKII
                     AYTSPDGDEWVTALEAKLKHREGMIYYSRHRPGTRKKMVLTRRKATNFFRYYSEADSG
                     GASAPESLTHLLCKQVLNELSNLPGGLTTVLNCTEHAEQQPPVTIRLNRALSEYRIDI
                     DGKTFYIDVLLEFDQPGNTSLLRHEIRWQRKLAVEIWHTSRLASNAPKCLALSKIGIP
                     VVQIRADKGSFLYIDEDELLNYDNEEIKNRINRHVEKLRNTFRKQILCTLLRNPLSAD
                     FQTALMLHNQIKADEQQAEQIQEKFEALRNKHVLLEAEYSALAAQYAALLEHQNFQAH
                     SGKREIPKKHGILQRMASWFKS"
     gene            39993..40607
                     /locus_tag="WM481_000190"
     CDS             39993..40607
                     /locus_tag="WM481_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079611.1"
                     /translation="MKKIMFGLLAAMAISNAQAEGNFLNLASKPAKIDGVLEASLDKP
                     YPKFVQDWMKDSKLAGLGRIIQGKIFESTGTIERKFYARSESEHEKTVEDAVGTFKAY
                     CEHHQGTVETLTSDIFCINGRQVQWMGWWYRENYTNFAFSDGYTGEQAYRSKVTDVQP
                     GKKISTSYGSALVLEKGENGLIKIQPNRGSERWITSDSIQVTYK"
     gene            40662..41024
                     /locus_tag="WM481_000191"
     CDS             40662..41024
                     /locus_tag="WM481_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079612.1"
                     /translation="MECKLKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEATLNLFV
                     EGFWIIGLLQALAFTTIVDYCTESKHTNMLNNPACNDEDWDNDMLNNPACNDEDWDND
                     MLNNPAYSWSMFNIHHSD"
     gene            41228..41815
                     /locus_tag="WM481_000192"
     CDS             41228..41815
                     /locus_tag="WM481_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HFA9079613.1"
                     /translation="MIGFVKWYSYEKDFGFIFGEDGHEYFFRKSAGSDIPGQIVNFEP
                     FEHSRGLRARKVSNIHNPDPQQIQKLKTLCKDFQKVEKQPMSKKYNLLSAAILSLCLT
                     MFITLGFFYILAGFFDTDRETNIVLLFVFPAVTFSVLTFFLNRIFRNMKPGSDTDVAT
                     NDDWLKDFWSEDSLIYDPAYSNLNCNIFHRSDDDI"
     gene            41862..42185
                     /locus_tag="WM481_000193"
     CDS             41862..42185
                     /locus_tag="WM481_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079614.1"
                     /translation="MKTKSKKLLRLVILFVAAFSISTPAVMYFSTGEVTLNLFVEGFW
                     IIGLLQAAVFTFVVDCSSSDLGSLSQWQIGNDWDRQMDPFYAHEIGNIWHGTHLDSSS
                     EEYWK"
     gene            complement(42242..42400)
                     /locus_tag="WM481_000194"
     CDS             complement(42242..42400)
                     /locus_tag="WM481_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079615.1"
                     /translation="MSIAQIVVQIEEVDEGNYQVRMSYSGGNDLVSEEGKSAFEQIIT
                     SIGKVLET"
     gene            complement(42404..42949)
                     /locus_tag="WM481_000195"
     CDS             complement(42404..42949)
                     /locus_tag="WM481_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFA9079616.1"
                     /translation="MWRGISSGRHVRGNYVLKASTAHNQAELERYVHNPQVRAFLDFI
                     AASEGVKHGYQTAIGNYRITDFSRHPGNSVRRSFTRTDGSRSTSDASGRYQFMGYTWK
                     GLAKQYGFTDFSPATQDKAAVALILAKKGAMEAILNGDYEQAVMKLGGIWASFPTAPN
                     EYRQHKRSWGFVHNFFRQRGF"
     gene            complement(42991..45993)
                     /locus_tag="WM481_000196"
     CDS             complement(42991..45993)
                     /locus_tag="WM481_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFA9079617.1"
                     /translation="MMAVEYFTFPGNGEAVRAVFNGVATISGSTMMGGAMQAAALFGF
                     LVTLAVAVFKLDLKDNFSYLFVVLFCWMGMMVPKTTVLITESGGYGYTGRQYTVGNVP
                     VGLAYMGYFVSSFGQSITRKAEQVNHLPDDLNYSRTGMLFGTRLMENIREARIPDAQL
                     TQDWALFMYQCSFFDMNLYHFYNVQDLAQSADILATLTHTNQALFTNVSQIASRRGNM
                     LQYNGKSKTMTCNEAAKELKDRTRFYSKNYLPGYIADRVFAGLGTNSAGINRVNALVT
                     LGNSSFQYLLDNARFDTLKNIEQAAMVEVIRQAGIINGQRNRNPAAVQQAFAQVQARN
                     QYIAAQKTGSSMASWNLPLIRSAAEAILIGLFPFVMILAMLGGIMAFRLLSFYMMSML
                     WIQLWAPVASIINMIMTMNAKRLFSVEAANGVITPGTGDTLLMAAADAQAAAGAAMWL
                     IPVIAGALAMGGRSLMNGMMGMTSSAKSTGEAAGSQVGSGNYSAGNMNYNNSNANKHS
                     LNPVYTDPQMMTAQSAAGTSWRNMATGDSRAQMRNSSFGVSSQSQISQSESYSKAADR
                     AETSAQQWQASFRESVSQGNANRLAFATNYGMDRSASEGYGMGLDAAESRKLSYVIDQ
                     GKKIAQKFGVTNTSAVISAIAAGLGISGNTGSSTKAQNIKNALGAKGDLGWSGKSQNT
                     DQMVAEIGESIEAGKRQGISFDSNFTDKVSQSEAYKVALNSGNSLAQTAEANFSKARE
                     ASHMASESYQKAQSYREMAAQTYGNGITIAQDNTNALVQDNPELTMSELNAGQGGTYN
                     AAIRAGVNSQDRLKAAAENSGWKPNDLSPEDRPQNNTQAAYKHFSDKVSSGAGAAFGD
                     VQTQAHQAGIIPDGISAAINARGENLKQEYANKSDKVEGDINQGRNSHRQEVGRLATH
                     AKNAQDQTNGVTMRINTHALPWNSFGERYDNAMKDVREGKGYIGSGSDQAAAHLGNPA
                     LASEFDLPIPQRPGNSKSSGSAVFRPKE"
     gene            complement(46000..47511)
                     /locus_tag="WM481_000197"
     CDS             complement(46000..47511)
                     /locus_tag="WM481_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFA9079618.1"
                     /translation="MKFHIFRFPKPAKVPTVCASSAKYLCLAASLALPINSSAGIEKN
                     MADMFNSMGAEANYTEAGAFHGQSGSLYTGGGMSVRFPVSDISLGNIQLPSISAGCGG
                     IDFFAGSFSFVNKEQFIQFTRNLGNNAAGVAFDLALKALDPMIQDAIGGIRDLVNYVN
                     QNSLNSCKLSQDIVGGAMGKLGSLASSNCRAASVDSGSSNDPAEGAWYCQAAKNLVHE
                     ADKVRNTGKPQDTISFTGGNLTYEAMKNYTGQRSKLGIDMDFYYSMLGTIVFTPLTQH
                     EGDKDKYTVGIQSFEPKILSIQDLLNGKNNAGGEIREKVVLDMWTCKDGSKKLMENCT
                     EKNDVEIPSLRYMVHKRIETLPTAIRNNQGWNLSTRKQIAAIVNNSKLPILKMAVSDA
                     FLGTGYLKKTAVLDAIAVDIMANILDQSERQVRSTLGMYNKTDDASGEAVRKLFANLG
                     ELRSRIYQERIDSMKKIEIEESMMRAMEQFETQWRSAFVDTNNSMTFDSMNRF"
     gene            complement(47683..48474)
                     /locus_tag="WM481_000198"
     CDS             complement(47683..48474)
                     /locus_tag="WM481_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFA9079619.1"
                     /translation="MKRVRMAVMLIFSLHASNLRAADDGMGWYDQDIWKNENRAFLWY
                     PDAKKPKQAGQNRPQNTPVRNELAEFEMLQRNLDESRKIAVMNPTPQNLKRYIELQEV
                     VMNKSAAFADQWQRVIWQNPNLDYSQRGRPNNQLAQQQYDHNRQQQKAGAIRKLAGEN
                     GIMFVFKSDCPYCHTMAPILKNFADQYGIKVMPVSLDGGGINHFPRALPNNGIAQRLN
                     ITTVPAMYVMDTKTKQFTPIGFGVMAQTTLEDRFLAYSRPVGTLY"
     gene            complement(48471..48896)
                     /locus_tag="WM481_000199"
     CDS             complement(48471..48896)
                     /locus_tag="WM481_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079620.1"
                     /translation="MNINNWLAASAGIVSGMFFTGMALAASLPYTGGIALEKTEALAR
                     TSIPLPDGKPDALLALAMRHGSAKGYFSGRAAAAVKQKFRQDIPIFVEAVRMEEIKNK
                     PGCNRVRLTFHTSPALSQTFPSEIMDIGACPNRKNGGKK"
     gene            complement(48883..50466)
                     /gene="traN"
                     /locus_tag="WM481_000200"
     CDS             complement(48883..50466)
                     /gene="traN"
                     /locus_tag="WM481_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFA9079621.1"
                     /translation="MRAITSLLVMICHFMLITSASAAALRESAACTRTSSVCVDGPST
                     KNINGVDVTKDCWEYKEEYQCLEKDSADYCAPLKDPSAKCEVQGQTCLEQSNEGECLR
                     YTHKYSCDVDLRTLHQGRLPTKVEEMEHTHLISSQWDESSCQVQGKKCKAVATECLEP
                     GSTKTINGVPVTRDCWKERRTVQCTDGSDSETCSAYTSSDQCRLIGDKCTHQLPDGTC
                     QAREKQFECTEKGETTKEVSGCQDRDFAKTMTTMEFARETQRFYDPEKQRFFNGEAGQ
                     CSIKLDGALDSVFGGDCCRTKADPGKFVDFAVQTGTTMATTYFMASVASHYTFTTMFV
                     SSAAQAMGTALSAAGGITGTSQIGALGFSAAGQQGMGVIVGFNPAVFAAAIAVIAIQQ
                     WLKCPQSEILVAMKRKADLCHYVGSYCGSKILGACVTMIESQCCFISKLAKIVNVGGK
                     EQLKRGWGTPENPKCEGFTAQELEQLDFSKLDLSGFYEEIYANMDNVAKQGQKVSQKI
                     REASVNGKNLEVKNYYEYQ"
     gene            complement(50463..51707)
                     /locus_tag="WM481_000201"
     CDS             complement(50463..51707)
                     /locus_tag="WM481_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079622.1"
                     /translation="MKRHAIYLTVFGLMQTAFAADYGSYVGELGKLGAGTATNMMQPA
                     QYTPRYTDNVPMAEQYYGGGVSLPTQFGDAKFNDCKNLPANPDLYLRQECEGVNLIAG
                     SRTKRPDVTISSNEKLVKGTQKIAGDPAETLEKYKWKYPVNEDGSVGTVPSSACPTET
                     ITVPAVKTVKRCNEYHGAEMFLCEAALKVNVDPGWNYSCLESKYRNQQYQCGKKLKVV
                     CEKSGPNCTRSGIEAGSLQGDMTVRLTASGSAHRLMFGTIGDNYWRDGQYDREMTLNV
                     KNPEKLTLFTLSRVEYDDWLIVKVNGKIVYSSYNNQMFAADYTAKDEDGFPIGRRFAP
                     VRNEEGTRLGNAERGKSWRKNLNIDIRPYLHQGKNTIWTRTVVGGGGENAIFFNVHQY
                     CEPVCHDKWENSCSEYEKRVKQ"
     gene            complement(51704..52456)
                     /gene="trbC"
                     /locus_tag="WM481_000202"
     CDS             complement(51704..52456)
                     /gene="trbC"
                     /locus_tag="WM481_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFA9079623.1"
                     /translation="MDKSLRNVIVAGGVFLILLGGLAGVGSAYADADVEAARNRLRTT
                     MGKVTRVKNPNVGDNRQQPEIPDLKGIKHRVPDVRDYGNAKIKADPMSIAQKYRTASQ
                     EARQAAEKADLMVFVSFSMPEASLKRIARETAKAGGVMVIRGFKDDSLKATVKAAEEL
                     AALQGDMLIHPELFDHYGITEVPVTVIARNSEDGLDGCAVNSELGMCTEHVAVKGDVS
                     LHSALEHLIRESEDKKLSDIANAKLAVLEGRK"
     gene            complement(52471..53589)
                     /locus_tag="WM481_000203"
     CDS             complement(52471..53589)
                     /locus_tag="WM481_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HFA9079624.1"
                     /translation="MKIFVTPLKLLIPLLLLPLFSISHAAEAVPTAPIPNKLTCQGKM
                     LNPINDICWSCMFPIKIGGKINLNMDNQEDNGSSPNNWFCSCTHPSRSGVTVSFWEPT
                     HLVEVVRTPFCMVSLGGISLKNQKADTAETTGKADVSNKIQNLFGGSHKRGRNEQHDQ
                     HNAFYQVHWYKNPVVFWLEALLDNDCMEKGTLDIGWMTELDPTWKYPSLAVYQAPDSA
                     LFANVVAQAACAADCVKAAAGFPFNKLYWCAGCHGTIYPLSGWMSGHFSEVQAAKLMA
                     ARIQTKMHRDLVAWRTSGRDALCGYKLNPVMDKQNYKMAMTYPVAARKFRGRCCEPIG
                     RSTLVRDSGKAFPYKGNQFAFQLFRKRDCCGAYKFKTN"
     gene            complement(53586..54242)
                     /locus_tag="WM481_000204"
     CDS             complement(53586..54242)
                     /locus_tag="WM481_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFA9079625.1"
                     /translation="MSHKKKIASVASLVILATGISSWVAASTPSVEKVGDKIGTTYPI
                     AEKHAIREIEARLKAKQDSGQLAAMQKEVQDRINRSALNLQPLEGLAKATKGSVRYFD
                     PSYTLTETIYDQDGQIIAPAGTVVKPLEAAPIPFKMFFFDGREPDQIELAKKLAAEYG
                     ESFMPILTAGNWYELSVEMDQAVYYDQEGRMSKSFQLNEVPSLVSQEGGRLRVEVMKP
                     "
     gene            complement(54251..54796)
                     /locus_tag="WM481_000205"
     CDS             complement(54251..54796)
                     /locus_tag="WM481_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HFA9079626.1"
                     /translation="MFEAMIKISQKESNKAVMKRLYREFCQKYATPFLAVAVCLSAFN
                     QHYALAFNLSRSLPHHLYFIKKDANKLSDLKQGDYVAFAWQGGFYPIGTQVVKEVAGL
                     PGNHVTKANRTFFIDGKEVGTAKEYSLDGMKLAINPFEGEIPQRFMWVKTGHKDSLDS
                     RYELSGLIHSGQIIGKAVPIF"
     gene            complement(54796..55344)
                     /locus_tag="WM481_000206"
     CDS             complement(54796..55344)
                     /locus_tag="WM481_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079627.1"
                     /translation="MTEAKEKKENTANTDPEKNPEDNGVEVSLGSLVIGAISIAALAL
                     GVWNFMKTHEKPVHIVDLAGISMTYREQAKNQGLQNGISDRQRAAILQNYQAKMNTLQ
                     QVIEEKVIRCGCNIFVKSALVGRNTEVVDVTADIMKELDKRVPATAVVAAPVHQQVEK
                     TIPKPMAEEGKGTPVTEEEMDR"
     gene            complement(55372..57948)
                     /gene="traC"
                     /locus_tag="WM481_000207"
     CDS             complement(55372..57948)
                     /gene="traC"
                     /locus_tag="WM481_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFA9079628.1"
                     /translation="MGVLSKFKNWLAGSDDVDQPMEALHNNPVDPDFDHLSDYLNYGA
                     YDTKTELFTLMSVKGDKPLGMGFILELNPFLGGSDDLIGKWGSLYALLPEKTPIQVQI
                     FGSPDLRNYFKEYEQVQASRPIDDPMRSTFETLAKKRTEFWNKGTQKVLVENTAIRLR
                     NLRCILSVNLNIPPDNAVEVSKAVSLLARMRDSLRSMQIYGRTWNADDLLAWTTLLLN
                     PNRMFTGQQDEIDPVWDETKFLSEQMIETRTSIKVLDSGSGLRFGNRAAGDCVIAQCY
                     SANRYPEEFHLSNMGALIGDVIEANMNYTSPFLISMAMFKRDYDTSSNTVKLKAARAK
                     QTAESKMAAVMPEAAKIKRDYDICLEAFGKGGGGLVSLLHQVVIWERPENINLAESQA
                     VSIWQAQGFGLYRDQYLQLGSYLTALPMAIDKEVEKYLDSKKRWSTKTMTNAVCMSPV
                     IGEWHGLGRPVIGLFGKRGQAMGIDLFANPAGNYNFAIIGASGSGKSFFANEIVRNYM
                     GLGTQVWIIDVGRSYEKFCQMIGGQYIEFTYEAGLCFPPFQMVTDINEDIEMLKLIFS
                     LMASPSDSLTEQQEAKLQRIIEEVWYEFGQEGTVDHVRERCLKCLITGSNEGGKPGER
                     DLSMVAVADQLYTYCSEGIYGSYFNGKLNVEFKNDLVVLELEELKTKPDLQQIIMQLI
                     MYRIMQGMYLSRSQYKIMMIDEAWALLGDTGTAARFIEEGYRRVRKYKGACGTLTQGA
                     NDYYKTKAAEAALENADFVFQLRTGAKSLKTIQDKEPFEVNDGIMQILRTLSKTDYYS
                     EIFVSTPIGYGVGRLYSDPFNVLASSSKAEDVEAVNHYRNQGLNTAEAIEAVLRDRAA
                     TR"
     gene            complement(57958..58539)
                     /locus_tag="WM481_000208"
     CDS             complement(57958..58539)
                     /locus_tag="WM481_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraV family lipoprotein"
                     /protein_id="HFA9079629.1"
                     /translation="MMRLKALSITAAILILSGCSTLTMSGIGGSEKFRCPNARNSDDP
                     YCESISSNYKASVAGVLKDGMKRQVGQPYTDQSVRTLMLTQAYSSGTPVRSQSEIARI
                     WVAPYLDTDGDLNDQSFTYVVLNQGDWLIAHNGQQIINEYRPIRLLGDGAKTSGSSVE
                     TSPTNSNNTNDRIPDVGVNLHLQETSGIPGTVR"
     gene            complement(58536..59255)
                     /locus_tag="WM481_000209"
     CDS             complement(58536..59255)
                     /locus_tag="WM481_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFA9079630.1"
                     /translation="MKKKILATIAAITAFQTAFADVKTVGSTLHKLYPNTTFSSVKAT
                     PMASIYEVTMGDNIAYVQENGRYFIFGALYDMQEQKDLTEMARSAVTQKSYSRLPFKN
                     AIKIVKGNGGKGKREFALFSDPDCPFCRRLEETLAGMTDYTAYVFMFPIKSLHPDAIS
                     KAEHIWCSKDREKAWNNYMLMDKEPAAGNCKNPVSENIALAEQLKVRGTPSMIHKDGR
                     RTSGAMPRAELEKWLNGAGAE"
     gene            complement(59265..60578)
                     /locus_tag="WM481_000210"
     CDS             complement(59265..60578)
                     /locus_tag="WM481_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFA9079631.1"
                     /translation="MRVKVNKFKLSAARKRQLAIAGMVAGSMAAIVGSAIYVIEGNKK
                     KSAEVTQVQNKRQILRTDFTSPQAGITDNSLWMNTESSKIEYANRKISELETMVQELK
                     EKENSSNPDTSKGLGPDGLGKPPAISGIGDNGRLPPAPPAGTLPNGAPPADRPIERKI
                     VSGSMSEAELQPGTTGSVNTGNPNENVRVSPQLKEAEWVKSKTPRMNIEVVEDGGKVV
                     VSQGKFRARDSYIPSGTFFRSVLLGGVDAPTGGEAQNASPHPVLMRVTDFAQLPNRFK
                     YNFRECFVTGQAYGDISSERAYIRLQNLSCVGTDGRAIDMPVKGYVAGEDGKTGVRGN
                     LVTKQGQLLANALMSGVISGMGKGVSEAFKVTNNTAFGSTTSIRGSDQYRAGIASGIG
                     GAADRLAEYYIKLADKVFPVVEVNAGRQVDVVLTQGIEIDTGETK"
     gene            complement(60580..61314)
                     /locus_tag="WM481_000211"
     CDS             complement(60580..61314)
                     /locus_tag="WM481_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFA9079632.1"
                     /translation="MCSLLKKIVFSALLAAGPATSFAAQRVPATENTPHVVSISKRNL
                     SRIAIEGGRISSWKFMEGDLELQKDTTTGQLFVRSLTSNPTNLFVISEEGKTYLLVLK
                     PTSKQGDNIVIDVAGANRREAAVLASQTGPMPVTMNSTEYVRAIKKMMASMMRGTTGD
                     MGINHSHEYQTVPLWKNTLFIQNGSYTAADMQGLSFTLTNLGTKQLEIREQEFYRQGV
                     LAVAVRKQILQPGEITDVFIISRPGG"
     gene            complement(61316..61966)
                     /locus_tag="WM481_000212"
     CDS             complement(61316..61966)
                     /locus_tag="WM481_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFA9079633.1"
                     /translation="MLSELAIRTGIKVFFWILLGMSIAVNLFLSATLLFKGTIVQTTL
                     TPPEIRRSMTVSNIAFSKEYLEEMAPYAAYLLLNVTPKTVDYQNSQLLKIVSPDYKDA
                     LERELSLNALWVKKNNVGTTFSATEATADISDNTVSIRGVFEVKRNNALVEQKDRELL
                     ISFKNNYGTLQLLSIKEVQKRVTKAEPAGQEKSEVETEDVEISKTATEFKGGNRGN"
     gene            complement(61978..62259)
                     /gene="traL"
                     /locus_tag="WM481_000213"
     CDS             complement(61978..62259)
                     /gene="traL"
                     /locus_tag="WM481_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFA9079634.1"
                     /translation="MRDYHVPVSIDDPKKAFIWDLDVFILFMSGFGIGIVSRHFFISM
                     SISLFIAWRWGKLKSGKHQWFFLHVLYWYLPINEKNKRIPETNKREFLN"
     gene            complement(62281..62577)
                     /locus_tag="WM481_000214"
     CDS             complement(62281..62577)
                     /locus_tag="WM481_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HFA9079635.1"
                     /translation="MNIKRNKLVKSASAVALALYANLAMAATTGAEFKGLADMVTGWS
                     EGYLGMALGLTAFLIGLAVGLMKQTIMPCIVGLGVGLAATLGPGIIKGMFTLII"
     gene            complement(62633..63154)
                     /locus_tag="WM481_000215"
     CDS             complement(62633..63154)
                     /locus_tag="WM481_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFA9079636.1"
                     /translation="METIKNTLFVASAMILTACAAPKQEVAGLPQDATYTVEQNQLGE
                     FVFCRNEECSGFERKVLAEQPVYAQTQTNLQKDEIRVFFNLGSSHLGAEGVRTLKRVL
                     PKLKNTQTVYLRGWADSIGGRNTAINRRLAKQRAAVIGKWLRKHNVSAKIRTASSPAC
                     CNSDDTRTVVITW"
     gene            complement(63124..63585)
                     /locus_tag="WM481_000216"
     CDS             complement(63124..63585)
                     /locus_tag="WM481_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9079637.1"
                     /translation="MRLRAILLQFVVPGVLVLTSARAVAFCYKEAGSKYGIDPAMLQS
                     IALTESAFRPNIESHTADIGLMGINRSWLPVLHKKFGLTGADVWNPCTNVHIGAWILA
                     NSYRQHGKSWEAVGAYNAACTKLKGRACYRARMTYANKVYQNWKRLKTRSS"
     gene            64096..64515
                     /locus_tag="WM481_000217"
     CDS             64096..64515
                     /locus_tag="WM481_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079638.1"
                     /translation="MNETDLPKLLSVINRFTDIDKNWSCVLLFWIQRASGYLEMMTLD
                     DDENTATFLIEKLEKLLEPLPIDIDNTTFAEALADDFEILFLMAQYGSEYWNSLEESF
                     EEFCIRHTTLPNQLIADIPHAKKEKVTMWLKSLLKSS"
     gene            64512..67064
                     /locus_tag="WM481_000218"
     CDS             64512..67064
                     /locus_tag="WM481_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFA9079639.1"
                     /translation="MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQN
                     IVNDRFRILNAHELIQVLDLSPQISGIKMNLGLSDENWSKDALPFLEKYIAFVQRLPA
                     SESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKTEEIAKKTAVWRYGIMCAALL
                     HDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKSAYSTHAEI
                     AWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLR
                     SGSRQRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNV
                     PDYIRQYLRKNQHPAAGSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMD
                     GEKVRNIFSVLCFNAKTLYPDGDYPTEFLGNLSEVSAKKEIPDVVVSEDKVSTQIQGA
                     EDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAFDNSMNTAAPAENKSSESE
                     KAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEVKTETVD
                     KPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTH
                     QIDSEAVSLPESESAQNGMRELEQLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAE
                     LEAIVSERNLKKQSETESTEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRS
                     GATVHFIEQGMLLVTPAVFRDYAGGVFNKSDTESLGPLTQKGFEALRLHARTKKSSLH
                     RVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLDESDLLVMEQK"
     gene            67061..69103
                     /gene="traD"
                     /locus_tag="WM481_000219"
     CDS             67061..69103
                     /gene="traD"
                     /locus_tag="WM481_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFA9079640.1"
                     /translation="MSAHFPENVYRSPYEWISVAAYSTTAVSVAHLPVPPTIAYGSLA
                     IFSLVSVKRALSALRIMRFRKNIRKLPYYALTADEIPSSNKALFLGKGFRWTQTHTQR
                     LFMVRQPENDGLRNPSKLYKAARKHEIETMGTDWISRITRKSAWWNPVAPLPPVGGSP
                     ELHGIEIEEDDIWEDLGERVGHKVVLGTTRVGKTRLCETMIVQDIRRGDVTIAFDPKG
                     DADLMTRMYVESQKSNRPFYMFHLGFPEYSCRYNPIGDYGRITEVATRIANQLPSEGQ
                     SAAFRDFVWRFVNVLTKTMEGLSIKPSYENIYKSAANVDELAGDYFKKILDKHHPGWE
                     KDFDNFDMPESEAKSAVKTGRNLDTMKLGSFLKAKKHNEPLIDALGGILSNDRSYFEK
                     LVSSLYPLLEKLTTGEVAKLISPDTEDLSDPRIIMDWRKVMESNAVVYIGLDSLSDAE
                     VAAAVGNAMFADLTSLAGQIYKFGHGVGQSGKTQKRKVSIHADEFNELIGDEFIPLLN
                     KAGGAGYQVTVYTQTWKDVEAKIGSPAKAGQIGGNLNTMIMLRVKTVETAEMLTNQLP
                     EVKIMTSTLVSRAGDVAFPDSHEDFSSGNEDRIGAETVPMLTPADLTQLPKGQAFALI
                     EGGQLYKIRLPLPKKDKQEEIPAHIGEMAADMREVYRLSRPSAEENAFTEGSSYAV"
     gene            69093..69788
                     /locus_tag="WM481_000220"
     CDS             69093..69788
                     /locus_tag="WM481_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HFA9079641.1"
                     /translation="MPSNTSAWYMNSLPLKFLFLPFKAAYTLFLIVLLIFGSSVLTQN
                     YFGTGNLLEDEIKWSESVGHDAFPGKEVDDTINLQIARLSYKGLKTVFFDLTLVNESL
                     KAKSEADFGYKFKNYLVPRMKYLQQFDTTLKVISLRLGNLALIVGISVIFAIPVLIDG
                     LVMRAIRQENASRESAGIYHRAKYWRTGIIWLGCMIYMCVPISIPAYLLYLPLVAAIW
                     MIFMQAKYLKKYL"
     gene            complement(70218..70730)
                     /locus_tag="WM481_000221"
     CDS             complement(70218..70730)
                     /locus_tag="WM481_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079642.1"
                     /translation="MKSKLPLILINLSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPAPSPSNGGQAVKYKAPVKTVSKPAKSNTPPQQA
                     PVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQKINALQSNVLDRQQNI
                     QALQRELGRM"
     gene            complement(70821..71216)
                     /locus_tag="WM481_000222"
     CDS             complement(70821..71216)
                     /locus_tag="WM481_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9079643.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRTLPPPFYFTGINIRLFAALGFL
                     SLSGAAAQASIYHCNSNGKSVYTSDPSGSCADADLPKISSHQGGGYRLKIKKLSQEAK
                     IHTGNKKKNKKHAGKKNRQAAKAPKENQK"
     gene            complement(71203..73125)
                     /locus_tag="WM481_000223"
     CDS             complement(71203..73125)
                     /locus_tag="WM481_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9079644.1"
                     /translation="MIEIKNLTLQRGLKVLLDKASAAVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDISIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     VQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYSGNYDFYQTERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVHSEKLNIGYFAQHQLDTIRADQ
                     SPVWHIQQLSPEVREQEIRNFLGGFDFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIVVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAATAPAASTQSQNRKDTKRIEAQIRQEKARRSKPIQQKIDKA
                     EKEMVQLSEIQTGCEAFLTQEDAYLEANKEKLQNTLSELAKVKTQLAQIEETWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(73202..73573)
                     /locus_tag="WM481_000224"
     CDS             complement(73202..73573)
                     /locus_tag="WM481_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFA9079645.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLPQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            73769..75076
                     /locus_tag="WM481_000225"
     CDS             73769..75076
                     /locus_tag="WM481_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFA9079646.1"
                     /translation="MKPVNIGLLGLGTVGGGAAAVLRDNAEEISRRLGREIRISAMCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKEAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     DGVMNAVRVNANMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            75069..75503
                     /locus_tag="WM481_000226"
     CDS             75069..75503
                     /locus_tag="WM481_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079647.1"
                     /translation="MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCMKNIPFAEKWQNDLKARGLDADNTRLAVDYCKCMWEQPLD
                     GLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKAD"
     gene            complement(75679..75948)
                     /locus_tag="WM481_000227"
     CDS             complement(75679..75948)
                     /locus_tag="WM481_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFA9079648.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTNALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTLKFRAGKALKDAL"
     gene            complement(76130..78592)
                     /gene="lon"
                     /locus_tag="WM481_000228"
     CDS             complement(76130..78592)
                     /gene="lon"
                     /locus_tag="WM481_000228"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFA9079649.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAKLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLGLPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVRNPLFLLDEIDKLGSDFRGDPASALLEVLDHE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELVVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLNE
                     DKKRLSETKKTSKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGMIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNVKDLEEIPENVKTGLTIHPVKWIDEVLALGLESRPESWAEP
                     SAAEAAAESASKPKPRSRAIKH"
     gene            78844..>79297
                     /locus_tag="WM481_000229"
     CDS             78844..>79297
                     /locus_tag="WM481_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFA9079650.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWYYVRESGADGILKATYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGVHEWHK"
BASE COUNT        20319 a        18019 c        18782 g        22177 t
ORIGIN      
        1 atactttccc gaagccgccg cccgattttt tgatatggcg gccggcgcat catttccgac
       61 caatcgaaac gcccgtattg aaagattgcg tttatttcca ccgccgtttt aaaggccgac
      121 ggtattcggc agacggggtg caaacggcgt tcagacggca ttttcattct ttcggcgtgt
      181 ccgtcagaat ggctttgccc gtccgcgcaa tcagcccgtg cggcttgcct tgaacgaaca
      241 aaaatgccgt ctgaaactcg gaaattaggt ttcagacggc atttgtcctg aaaggctgtt
      301 caaatcagcg gtggtaattc ggcgcttctt tggtaatttg aacgtcgtga acgtgcgatt
      361 cgctcatacc tgcggaagtg atttccacaa attcggcttt ttcgtgcatt tcggcaatat
      421 tggcgcaacc caaataaccc atactggaac gcagtccgcc ggtcagttgg tggatgatgt
      481 tcacaatcgg gcctttgtag ggaacgcggc cttcgatgcc ttcggggacg tatttgtcgg
      541 tgctgtcggt tttgtcttgg aagtagcggt cggcagaacc ttggctcatc gcgcccaagg
      601 aacccatacc gcgataggat ttgtacgagc ggccttggta gagttcgatt tcgcccgggg
      661 cttcttccgt gccggcaaac ataccgccga gcatgacgct gtacgcgcct gcggcgaggg
      721 ctttggcgat gtcgccggag aagcggatgc cgccgtcggc aatcagcgga acgcccgtgc
      781 ctttgagggc ttcggcaacg ttgtgaatgg cggtcagttg cggcacgccc acgcctgcca
      841 cgatacgggt ggtgcaaatc gatcccggac cgataccgac tttgacggca tccgcgccga
      901 cggtgaccaa atccaatgcg gctttggcag tggcgatgtt gccgccgatg acttggatgt
      961 gcggataggt ttctttgacc caacgcacgc ggtcgatcac gccttggcta tgaccgtggg
     1021 cagtatcgac gacaatcacg tccgcgccgg cttcgaccaa ggctttgacg cgctcgtcgg
     1081 tgtcgccgcc ggtgccgact gccgcaccga cgcgcagacg gccttcggag tctttgttgg
     1141 cattgggaaa ctcggtggtt tttaaaatat ctttaacggt aatcaagcct ttaagctcgt
     1201 ctttttcgtt caggaccaaa acgcgttcga ctttgtgcgt gtgcatcagt tcgcgcgctt
     1261 cgtctatgct tgtgccttcg gggacggtaa ccagacgttc gcgcggggtc ataatggcgg
     1321 aaacgggcaa atcgacgcgg ttttcaaaac gcaggtcgcg gttggttacg atgccgacga
     1381 ctttgccgtt ttcaacgacg ggcaggccgg acattttgcg tttgcgctgc gcgcgcattt
     1441 ccaagacttc gcggatgagc gttgtcggcg caacggttac ggggtctttg accacgccgc
     1501 tttcgtggcg ttttactttg gaaacggcgc gcgcctgcat ttcgggcggc atgtttttat
     1561 ggatgatgcc gatgccgcct tcctgtgcca tcgaaatggc gaggcgcgcc tcggtaacgg
     1621 tatccatcgc ggcggaaagc agggggaggt tgagtgtgat ttcgcgggta agcttggttt
     1681 gaagtttaac gtctcgcggc agcacggtcg aatgtgcggg aaccaacaaa acatcgtcga
     1741 aagtataggc tttttctacg atacgcataa tgctcggtct ttcggtttgt gcaagatgca
     1801 cggcattata gcacgttgcc ggcggcttga cagtttatat tgtttgcaat atcccccgcc
     1861 ttcagacggc atacggcgtg gcggcgggat gccgtttgaa aaccggcagg gtgcttatgc
     1921 caactgttcc agcaaagcct gtttcaatga ggactggatt tttgggtttt tcaagtcggg
     1981 gctgaagacg gtaaagctgt cttcgacgcg ttcgtccaac gtggagattt tggcgtagcg
     2041 caggctgacg ttgtgggcga agaagacttc cgccatgtcg gcgagcagga aggggcggtt
     2101 gacggcggtg atttcgacgg aataccggtc cggatagtct tcttcggggg tgatggtgat
     2161 gcttggcgca ataggcatat agcggctgcg gcggctgatg cggcggttgc agctttgggt
     2221 ttcggcaacg gtgtgtccgt ggataaagct gttgagttcg gcttcgagcg cgctttggat
     2281 gtcggggtag tcttcggggg cgtgctgcga ggggatttgc acgatgaagg tgtcgaggat
     2341 gtagtcgtgt tcggtgataa aggcgcgggc ggcgaggata tcgaagccgt ggcggctgaa
     2401 gatgcggcag aggcgggcga acaggcgcgg gccgttgggc ataaagacca tcacttgaaa
     2461 gctgtcgctt tggggtagga tgcggctgcg gacgatgggg ggttcaaagt cgtgtacgag
     2521 gttggcggcg tgccacagga tttcgcggga ctgatggcgg gcgaaatagg cggaaccgag
     2581 cgcgttccat agttttttct gctgtttttc ggggacggcg gcgcgggtaa gtaagtcggc
     2641 ggcttcctgc cggcggcggc cgaagagggc gtgcgggttg ccgtcgttgc ctgcgaggca
     2701 gcgtccggcg gcgtggaaca ggctttccag caggctggcg cgccaggcgt tccacagctt
     2761 gggattggtg ccgcgtatgt cggaaatggt caggaggtag agcgcgctga ggcgttcgtg
     2821 ggtttggacg cgtttgcaga aggcatcgag tacgccgggg tcttggatgt cttctttttg
     2881 ggcgacggca gacataagga ggtggttttc aaccagccag gcgagcaggt cgctttcttc
     2941 ttcggtcagg aagtggtcgg cggcaaattg gcgcgcgtct gcgatgcctt gtacggcatg
     3001 gtcgccgccg cgtcctttgg cgatgtcgtg gaagaaggcg gcaaggtaga ggatgtcttg
     3061 tttttcaaag gactgcatca gcgcggaggc gtagggcagc tcatggctgt gcatatccag
     3121 ggcaaggcgg cggacgttgc ggacgacggc aaggatgtgg tcgtccacgg gatagatgtg
     3181 gaacaagtcg tgttgaagca ggccgacgat tttttcccac gcgggcagat agcggcccaa
     3241 cacgccgtag aggttgagaa agcgcagggt ttgggtcagt ccgttgccgc tgcggaaaaa
     3301 accggcgaag cggcgacggt tttcagaatt ttggtagaag ctgcggttga ttttgcgcgt
     3361 cgccccccac catgcgcgca gggtttgcgg ttcgagcgca gtaatgtcgt tgcgctgctg
     3421 catgatttcg acgattttga aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg
     3481 cgcggcgatt tggttgttga cttggatgta gtcgtcgtca atccgcaggg taacgcgcat
     3541 cggcgtggag gaaacgcggc ttcgcagcat gggcgtgagg atgccgccca gttgtttgac
     3601 ggttttgacc gcacggtaaa acacgcgcat cagttcttcg ctttgacggc ggcggttttc
     3661 atcttgataa cccatacttt cggcaacttg cggctgcaaa tcgaaaagca ggcggtcttc
     3721 ggcgcgcttg gcgtttaaat gcagccggat gcggatgtgg gcgaggcggc ggtagccgtg
     3781 cgaaagcata ccggcttcgg cacgtgtcaa aatccgctgt ttgagcaggt cgggcaggtt
     3841 ggccgccaag ccttgcgcct tcgctatcca aagcagggtg tggatgtcgc gcagaccgcc
     3901 cggacagctt ttgatattcg gctccaacac cgcccccgaa ccttgcgatt tggcgtggcg
     3961 gtgttccatc tccaccaatt ttgcttcgat aaatgccgct acattgcgtt gcacgttcat
     4021 tttttccgcc aattcgtctg ctgtttggcg gttgccaaac aaaaacctag cctctaaaaa
     4081 cgctgtgtcc cccgtaatat cgtcgcgcac gctttcgcac agttcgtcaa cgctgccgct
     4141 ttttacagac ggcattagtt tgcaatccca cagagtttgg ataaatcggg caatctgttc
     4201 ctgaatgccg tctgaaagcg gggcagggga gacaaccgcc aaatccacat ccgaacaggg
     4261 atacggttcg ccgcgcccga agccgcctac cgccatcagg cataacgcgc tgttttgaaa
     4321 atgttctgcc cacaatgccg ccagcaaggt ttcgactgcc gccgtgtatt ctctgaaaaa
     4381 taccgacacg cggttggctt tcaaataatg cgcttcggcg gcattgcgct gctgtttgaa
     4441 ggtttccagt gctgaagaca ggttttcagg catttttatt ctttcgattg gcgggaaaaa
     4501 gggaggcgga tggttcggcg gtcaaatacc gctttcagac ggcacttgtc gggtatcggc
     4561 ggaattggtc atcaggttca accgttcctg cggcggcagc agcaactgat acgccgccac
     4621 gcccaaagcc gccgccattt tttcgatatt cgacagggca atgttccagc gtttgcgctc
     4681 gactgccgac acataagtcc tgtccaaacc gcattgccgc gccaattcct cttgcgacca
     4741 acccttgttc acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc
     4801 gtcaggcaat tcggcaggca gagtcaattt gttgcccatc atttgtttcc ggataaatgg
     4861 ttaaaagtta agcatttgct gttacggatt ttacttcaca taaaagccaa aaaaaagggg
     4921 tggcggcagc cacacccaaa cacacacaca catcaagagg aaagaggtaa aatcaagacg
     4981 gattgggaaa cagcaaacaa acaatctaga tttcatatcg gtattatcaa acaaaaaccg
     5041 ttatcgagta ttgattcaaa tcaatttaga ttttattcgt aaaccggcaa agaaaaacgg
     5101 catacccgtt tatacggata tgccgtctga tgatgcaggg gactgctgta tttggcagtg
     5161 gtttgtgttt tagtcctctt gcgccgctgt ttgcaggtag ttcggcaaac cgattttgcc
     5221 gatcagttcc tgctgggttt cgagccagtc gatgtgttct tcgttagtat ctttttgttt
     5281 ttccaacaaa tcacggctga cataatcctg ttgcgcttct gctgtggcga tggcggcaag
     5341 cagggcttcg tgtttttcct gttctttggt caaatcgcag gcgatgattt cttcggtaga
     5401 ttcaccaatc aacagcttgg ccagctcttg caaattgggc aatccctcaa ggaagaggat
     5461 gcgttcaatc aaatcgtcgg cagctttcat ttctacgatt gattgtttga agaaatgttc
     5521 gcccagttct tcaaagcccc agtttttcaa aatacgggcg tgaaggaaat attggttaat
     5581 ggttaccagc agcaagccta agtttttgtt cagctcgcgg ataaccaaac ggtcgccttt
     5641 catacggact ccttttattc ctaatacgga ttacagttgg ctttggtagt agttgccttc
     5701 gccaatcagc tcgatcaggc gcagctgctg ttccaaccag tgtgcgtggt cttcttcggt
     5761 gtctttcagt tgggcaacca tcaggtcgcg cgtaacatag tcttgagcct cttcgcacag
     5821 tttgatgcct tttttcaaag cgtcgcgcac ttcgtattcg gtttgcaggt cggctttgag
     5881 gcaggaaacc acgtccgtgc cgatattcag ttcggcgcgt gccattttcg gcgtaccgcc
     5941 cagcatcagg atgcgtcgga tgaagtcttc ggcgtgtgtg gtttcttctt ccatctcgtg
     6001 attgagacgt tcaaaaagtt tggtgtagcc ccattcggag tagaggcggg agtggatgaa
     6061 gtattggtcg cgtgccgcca gctcgccgga cagcaattcg ttcatgtaat caacaacagc
     6121 ttgattgcct tgcataatat ctctcttttc ttaatttggg tttcgtgcgg ttaaacggcg
     6181 ttctgtaacg ctgttcggga tgcgtgcatt gtatgcaaaa gctgtcggca tgacaaattt
     6241 tctatttaaa atacaaacaa ttatcaaaat aaatagggcg tattccccga tgccttccaa
     6301 atcagtatgc tgtttcttat cggttttttt tgctgctaaa actaagaatc catctcatca
     6361 ataaagaaca tttacacttg ttaaccatag caaaaaacaa atagagcacg gttttttatc
     6421 aaaatttata atgaatcttt ctcattaacc gacagattct tttgagatta tcggattttg
     6481 ggaataaatt atgccgtctg aaggactttc agacggcatg gacaattcaa attgatgttg
     6541 tttacatcaa acaaggcggc agacagcagc gtaggaaatt attttttact tcggaacggt
     6601 gttccgagtt taatgatgcc cgcaaccgcc gtggctttcg cgtaaggctt ccgccgcctg
     6661 ttcgtcggca tggtaacttg aacgtaccat cgcgccgatg gcggcattgg taaagcccag
     6721 ttcgtatgct tctttttcaa atattttgaa ttgttcgggt gtaacgtagc gcaagacagg
     6781 caagtgtccg tctgaaggct ggaggtactg tccgatggta atcatctcga tattgtgcgc
     6841 ccgcatatcg cgcataattt cacgcacgtc ttcgtctgtt tcgcccaagc ctaccataat
     6901 gccggatttg gtcgggatat gcggcatcat ttctttataa cgttttaata agtctaaaga
     6961 atgttgataa ttggcaccgg gacgggcttt tctgtacagg ctcggatggg tttccaagtt
     7021 gtggttcatc acgtcgggcg gggtttcggc aaggattttc agtgcgatgt ccaagcggcc
     7081 tcggaagtcg gggacgagga tttcgatttt ggtgttcggg ctggtttcgc ggatggcttt
     7141 gatgcagtcg gcgaagtgtt gggcgccgcc gtcgcgcagg tcgtcgcggt caaccgaggt
     7201 gatgacgacg taacgcaggt tcatggcttt gacggattct gcgaggtttt tcggctcgtc
     7261 ggggtcgagc atattcggac ggccgtgtcc cacgtcgcag aacgggcagc ggcgggtgca
     7321 gatgtcgccc ataatcatga aggtcgccgt gcctttgctg aagcattcgc cgatgttggg
     7381 gcaggaggct tcctcgcaaa cggtgtgcat cttttgttcg cgcaaaatgt ctttgatttc
     7441 aaagaatttg cgcgatggga gtttggcgcg tatccattcg ggctttttca gtttttcctg
     7501 aagggggacg actttgatgg ggatgcgggc ggttttgtcc gcgcctctga gtttgatgcc
     7561 gcgtttgggg tcgtcggttt tgatttcact cattgttgtc tgctttcggt gtgagttgtg
     7621 tttcaaggtg tgcggtgagt ttggaggcga cttcgtccgg cgcggggcag ggttggacaa
     7681 aatccgcgat ttgcgtcatt tccataccgg cgtagccgca ggggttgatt tgggtaaacg
     7741 ggcttaaatc catattgacg ttgagtgcga gtccgtgata gacggaaccg tttttgatac
     7801 gcagccccag tgaggcgatt ttgcgttcgc cgacgtaaat gccggggcgt ttgggatctg
     7861 ccgccgcttc gatgccgtat tccgccaatg tggcgatgat gctgttttca agcgcggaaa
     7921 cgatgttgcg gacgctggtt ttgcgccgtt tgaaatcaat catcgtataa acgaccaatt
     7981 gcccgggccc gtgataggta atctgcccgc cccggtcgat ttggacgacg gggatgtcgt
     8041 ccctaatcag caggtgctcg ggttttcccg ccagtccttg cgtaaagacg ggcgggtgtt
     8101 cgacgaccca caattcgtct tcggtgtctg cattccgtcc ggcattaaag gttttcatcg
     8161 cttcaaaagt cggcagatat tcgaccaaac ctttgtgtat gattttcatc tcaaagtacc
     8221 actttgacca gttcgtgcga agtcagcgcg cggtagatgt tgtccaattg ttcttggttt
     8281 tcaaccttta cctgaacggt ggcaccggta tagttgcctt tgctgctcgg acgcgtggtg
     8341 atgtggtgcg cctgcgtgtc gggggcgtga aggcggacgg tgtccaatac cgcctgctcg
     8401 aactcgggat gcaccgcgcc catcactttc aatgggaagg cgcagggaaa ttcgatgagg
     8461 gatgctttgt ttttttgttc ggtcatgatg tgctgcctta tcgtgtacgg tatgccgtct
     8521 gaaggcgggt ttgcctttca gagggcatcg gatgtgcagt attttagcct aaaccacgat
     8581 aacaggctat cgggaaggcg gaggcttttt tgacggcgcg gcggttctgc tatactggcg
     8641 cgcaatatta ttttcggaag ggtggatttt atgtatcgga gaaaaggacg gggcatcaag
     8701 ccgtggatgg gtgccggcgc ggcgtttgcc gccttggtct ggctggttta cgcgctcggc
     8761 gatactttga ctccgtttgc ggttgcggcg gtgctggcgt atgtgttgga ccctttggtc
     8821 gaatggttgc agaaaaaggg tttgaaccgt gcatccgctt cgatgtctgt gatggtgttt
     8881 tccttgattt tgttgttggc attattgttg attattgtcc ctatgctggt cgggcagttc
     8941 aataatttgg catctcgcct gccccaatta atcggtttta tacagaacac gctgctgccg
     9001 tggttgaaaa atacaatcgg cggatatgtg gaaatcgatc aggcatctat tattgcgtgg
     9061 tttcaggcgc atacgggcga gttgagcaac gcgcttaagg cgtggtttcc cgttttgatg
     9121 aaacagggcg gcaatattgt cagcagtatc ggcaacctgc tgctgccgcc cttgctgctt
     9181 tactatttcc tgctggattg gcagcggtgg tcgtgcggca tcgccaaact ggttccgagg
     9241 cgttttgccg gtgcttatac gcgcattacg ggtaatttga acgaggtatt gggcgaattt
     9301 ttgcgcggtc agcttctggt gatgctgatt atgggcttgg tttacggttt gggattgatg
     9361 ctagtcggac tggattcggg atttgccatc ggtatggttg ccggtatttt ggtgtttgtc
     9421 ccctatttgg gtgcgtttac gggattgctg cttgccactg ttgcagcctt gctccagttc
     9481 ggttcgtgga acggaatctt ggctgtttgg gcggtttttg ccgtcggtca gtttctcgaa
     9541 agttttttca ttacgccgaa aattgtagga gaccgtatcg gcctgtcgcc gttttgggtt
     9601 atcttttcgc tgatggcgtt cggagagctg atgggctttg tcggaatgtt ggccggattg
     9661 cctttggccg ccgtaacctt ggtcttgctt cgcgagggcg cgcagaaata ttttgccggc
     9721 agtttttacc ggggcaggta gacggttccg aaacatattt gaagcggaat acaaccctgt
     9781 tcgggtttaa ataaaaatac cgtctgaaac ccgaaaacag agcttcagac ggtattttca
     9841 tcacggctta tttggcggtt ttgctgctgt cgataatttt catgccggca gaaatcaggc
     9901 tgccgatgtc ggcaacattg gcgggcataa tcagcgtatt gctttctttg gcaagattgt
     9961 tgaacgcggc tacgtattgt tccgcaatct tcagattgac cgcatccgcc ccgccttggg
    10021 tttgaagggc ggcggcaatt tgacggatgg cttcggcatt ggcttcggca acaaggcgca
    10081 gggattccgc ttcgcctttg gcgcggttga tgcgggcgat tttctcggca ttggacgcat
    10141 tgaccgcagc ctgagcctcg ccttcggatt gttggatttc ggcttcacgc tgaccactgg
    10201 caaggttgat ttgttcgatt ttacggcctt cggattcggc aatacgggcg cgtttttcgc
    10261 gttcggcggt aatttgtgcc tgcattgcgc gaaggatttc ttgcggcgga accaaatcct
    10321 tgatttcgta acggaggact ttcacacccc aagccccggc ggcttcatcg agggcggaga
    10381 cgacggtact gttgatttcg tcgcgttctt caaacgtttt gtccaactcc atacgcccga
    10441 taacggaacg cagcgtcgtt tgggcaagct gggtaattgc cataatgtag ttgctcgaac
    10501 cgtatgaggc gagtttggga tcggttactt ggaaatagat gatgccgtca acagtcaatt
    10561 gcgtattatc gcgcgtgatg cagacctggc tgggtacgtc taaagggatt tctttcagcg
    10621 aatggcggta ggcgacgcgg tcgataaagg gaatcaaaat attcaaaccg gccgtcaggg
    10681 cgcgatggaa acgcccgagc ctttcgacaa cgtggacttc ctgctggggg atgacgacaa
    10741 aggatttgaa gccgaaaacg gcgacggctg ccaacaagat aatgaaaaat tccataattc
    10801 ctccgagtgt taagggtttg cgatgataag aaggttacct tctttgcgga cgatgagggc
    10861 gcgcgttccc ggttcaaaca cttcctgccc cgtattttgc gcctgccagt gcgtaccgcg
    10921 ataaaaaact tcgtaacggt tgccgcctgt gtatcggagg atttcggcat attttccggt
    10981 atccaaatcc tgatatgaat ccgtttcaac ttttcccacg gcggttttgg catgtacgaa
    11041 ccaaatgccc agcgcggaaa gcagtgcggc ggtcaagacg gcggcaggcg tgctgccagt
    11101 cagcccgtag gcaatgcccg aacccgccaa agccgcgctg acaaccaaaa gataaaccgt
    11161 tcccgtcaat aattcgatga ttaagacggc aacagcggca acaaaccata cagtcataca
    11221 tttccccaca aagcgcgtcg tttgacaaaa taacgcaata tcagcagtat agccgaattt
    11281 gaaaggatag ggcagatatg gacacttggc acgatgcact cggcggcgaa aaacagcagc
    11341 cgtattttca ggaaatttta aatgcggtca ggcaggaacg tttgtcgggg caaattatct
    11401 atccgccgga ggccgatgtg ttcaacgcat tccggctgac agcgttcgac cgggtcaaag
    11461 tcgtcattct cggacaagat ccgtatcacg gggtagggca ggcgcacggt ttggcatttt
    11521 ccgtccggca gggtgtccgc ataccgccgt ctttactcaa tatttacaaa gaattggaaa
    11581 ccgacatcga aggcttttcc attcccgcgc acggctgcct gacagcgtgg gcggagcagg
    11641 gcatattgct tctgaacacg gttttgacgg tgcgtgcagg acaggcgcat tcgcacgccc
    11701 ttttaggctg ggaacgcttt accgataccg tcatcaggca gcttgcgaca caccgcaagc
    11761 accttgtctt catgttgtgg ggtgggtatg cacaacaaaa agggaggctg atagacagtc
    11821 aaaatcattt gatactgacc gcgcctcatc cgtctcctct gtcggcatat cgcggttttt
    11881 ttggctgccg ccatttttca caggcaaaca gctatttgag tcaacacggt atcgaaccga
    11941 taaactggaa gctatgaatg ccgtattcaa gcgtcaaagt ttgcgtgtca ggttcatttg
    12001 gcggaattcg aacagctctt gatgcaatga tcgtgtttta aggtttcctg caataccgct
    12061 tgtaaagctg cgactgccgc aaagccatag gctgcgggct tcactattcc gaacgctttg
    12121 tgcaaaagaa gcaacatcat gaaattcaag caatttattc ttgccactgt tttgggcgcg
    12181 acagcctttt ccgcttgggc ggacgattca taccaacata tccgtgcagt cagaatcctg
    12241 ctgctgatat tgtctgtttg gtgcgtaggg tagggaatcc gccaacgtaa aaaccgcccc
    12301 gccaacaccg ccgtcaccaa attgggcatt gggcgtatgc cctgatgctc gctgttccgc
    12361 cgcaggcatt gcccgtcaaa tcggcaatgg cggaggaatt cacggcacgc tggcattttc
    12421 attgctgttt ttgtcgctgg gacatattgg aatggttgcc taccaccaca tcaaaggcga
    12481 atccgttctg cctctgttct tgcttatcta ggttattgca aaatatgagt ttgaaagatg
    12541 gatatttttg tttgcttttt tatttgttct gattaaattt tttaagtgtc tgagttttga
    12601 atgagattgt aaaaatatct attcaatcca ttataaattt aacataatat acattatgcg
    12661 aactagaata aatatgtaaa tttcatattt taaacctgtt cgattgcgga tgtttttctc
    12721 tttataaatc aattaaatat aaggaaatat tatgtccgca cccgtaatat tgtctgcagc
    12781 cgctacaaaa ggtggcgtag gtaaaacaac actcattgct aatgtctctg ctgttctcgc
    12841 tgatatcgga ctgcgtgtct taatgattga ctgcgatgtt cagccatcat tgtcaaaata
    12901 ctatccaata agccaccgcg ctccaaatgg tattgttgaa ttacttctcg gtgaaaatac
    12961 cgaagaaatc atccgctcga caatttccaa taccgttttt ccgaatttag acattgttat
    13021 ttccaataac atttctgctt ttgtcgagac tgaagtaacc tctcgtcccg accgtgcctt
    13081 cttgctcaaa agcaaactgc attcccaata catacagaat aactacgatg tggttttaat
    13141 cgatactcag ggtgcagttg gtcctctctt agataccgca gcatttgctg ctacatgcct
    13201 gctttgccca attatgcccg aagttttgag tgcccgtgag ttccttaccg gtacgcaaga
    13261 acttctaaag cgtctgcaaa aaggcgaagc aatgcgtatc ccgatgcctc aattacgagc
    13321 catcatctac gcacaagatc gtaccaatga tgcgaaattc atcgcatctt ctatcaaaga
    13381 tttctttagc aatactctag atgacagacg taaattgtta agcgtagcag ttcctgctgc
    13441 caaagcatat aaggaagcta caactttaag agtcccggtt cattgtcatg agcgtaaaca
    13501 gcttggaaag atgctgcctg cttatgaaat catgcaccgt attgtttatg agattttccc
    13561 tggtatcgaa cagaagaacc tgcgtggaag ctgtttcaat gacatggcca gcctgctgca
    13621 aaaggaggtg caatcatgaa tttggaccaa aataaagcaa ccttgagcct gcacatcggt
    13681 acccctgcac agacaaatat gagttttggc caagaggata ctccttctcg gggcatgtta
    13741 atgtcgttgc caatcagcga aatcgatttc tttgacaaaa accctcgccg tttacatgat
    13801 gtttccagct acgcggccat caaagagtcc atccgttctt caggtattca acaacctgta
    13861 cacgttaccc agagacctgg tgaaagtaaa tatgtactgg cacagggtgg taatacccgt
    13921 ctcaaaatct tgtgcgaact cttggaagaa actggtgata accgcttccg tatgatgcct
    13981 tgtatttaca tcccctactc tgacgaaact acattgcagg ttgcgcatct tgttgaaaat
    14041 gaactccgtg ctgaaatgtg tttttgggat aaggcctgta aatatgccga aatccgcgat
    14101 attttccaag atgaatctga ctcagggaaa ctttctttgc gcgaactgga agtgatgttt
    14161 tctgataaag gccttacggt ttctcataag actttaggat tattcttttt tgcctccgat
    14221 aatttacaag agctgcaaga atacgcattc aaactttccc atgcaaaagt agaagaaatt
    14281 aaaaaacttc actctaccct attctcaatt tctaaatcaa ttcagttgga cgattctttc
    14341 caatatttgt ggtcggatgt actggagcag tggcataacc aatattctgc aacagagtta
    14401 gacgtgcatg agctttatca gttcgtgctc gacagattta aatttgaata tggctctgac
    14461 gccttttctg aaacagaaaa ttcttctgat aaagacaaaa ttgaaacagc cgccaaacaa
    14521 gatgtcaatt tgcatcaaaa ggattcggat atggaatttg tgccagccaa agtaaatggt
    14581 actaaagctg atgcagagga tgagccgaaa gaagccgaac cgattacttt ggagcaaacc
    14641 agcaaaaaac tgttttctgc tgttcgtaaa ctgttagcca aagtgagtct gtccgattgc
    14701 tttaagattc ataaggactt tccatacggc tggtatgtcg aatatccggc ttttgagcat
    14761 atttcagcat caagtgccgg aaatgccatc tatgctattg atgttttgca tgaagaagcg
    14821 ggaaatgttt tcacattctt atggaagctt agtggaatcg acacctttat gtttgatgtt
    14881 ggtgatagtt ccaatcccct gctgacactt aaaagtgatt ctaagcttcg tattgcatac
    14941 gaggaccccg ataagtacga cgaatatacg aactgcggaa ttggtaataa agagaacatt
    15001 attgcctctt tactgaattg gcaaactaat accagccatg aatttcacgc caatatcgta
    15061 gaaatattat ctctgagcac acagtacaac aacttgagaa aaacgggagc taagagtgag
    15121 gagtaagcct ttaattagta atcccattta tagactgttt aatacggatc cggtttatac
    15181 caatcttgca cttacaaggt tggcatccgc accaaatacg ccgcgtgaat cttcttataa
    15241 cgctttggtt gctcgtgaac atgaggatgt tttgaacacg ctcaaacgaa tgaacccgaa
    15301 gcggattgag aaattagcat ccatgtgtcc tgatgttgtg acctactcca ttgatctgaa
    15361 ggttttaaaa agcaaaattt cagaaatgga acattacgaa caaaggttta tggaacagat
    15421 gcagcgtctt aaatggatgt tagaacataa ggccagcaat ctgatgatta tgaatctatg
    15481 ttcattaatt tctacggcgg aaattaaaaa attgcgtata gaaatgaata ttcctgtcgt
    15541 taaagggcgc attgtgatgc catctattga tgttcgcgtc agggtattga aggcatggag
    15601 taagtcggaa aaagagccag atttgtttat tagataccag ctattgattg atgaattccc
    15661 cgattatagc cttgcccaat tacattcaat aattaacgat gtaaagttct ttggagtatg
    15721 agtatgtata tggatttctc tacttcctat gattatgaga ctggttttgt accatctaag
    15781 aacatgtcaa ccgataactt tatccgtagc ttggttgcct tgtgggagga accggccaca
    15841 tatgtgcgtc agtttgctca ggcagtcggt tctgtttcag agggatattt tttgagttgg
    15901 tttgtcgaga aatgtctgcc tggcgaatgg gtaagcctga ataatgatct gttgtttaat
    15961 gaagcaggta ttaccgcaaa gcagtggcgg aatattcgca atagccttct gcagaaaggt
    16021 attttgctta atcggagaac cgtagcgcca accgaatcac aattcacatt gaatgatgct
    16081 ctttttgagg atttggtaag taaacatggt aaatcagatg tacttgacat ggtttcgccc
    16141 cctatttcga ttaataaact tcacctgaag acattagccg atagaggtct tgcttttgta
    16201 tccattttgt atttgtcgtt tcttcaaagt gaagctgaat atcaagattt gagtaagcgt
    16261 ggtgactggt ctccttggac tcatgcagct gaaaagcacg ttttttattc aacaatgttg
    16321 tcaagacggc aacaggaaag agctattgcc gaattaacct cttttggttt gattgagagc
    16381 tgtgttgatg gtatgccagc agcgcgacgt tgtagataca gcttgaaaat gcttgctgat
    16441 cttacttctg aatatttgaa cggatgtgtg tgatggttac cgccaccaaa ttaccccctc
    16501 ccaaagtatt taactctgaa ctgacacaat gctggcaagc attgcaaggt ggtcgaaggg
    16561 ttgccatcta tcgatgttta attgatatta caggctctct gaagactgct gcaatgcttt
    16621 ctcagttaat ctattggaca agagtaagca aagaggttgc cgaacgggat ggttggattt
    16681 tcaagtctat tgctcagatg gaagctgaaa ctggtttgac agtgagagag cagcgcactt
    16741 gtaaaaacaa gcttcttgag acaaacctta ttcaaacatg ccgtaaaggt gtgggggctg
    16801 ctttagcaat caaagtgaac ttggatgcca tagctgagtt gatcgctaaa tcgagcggta
    16861 ctgacgatca acttgcactt acgctggctg atttacagaa cacaagttcc ctgtatttta
    16921 gaaaacactt ttctaaaagg attgcttatc accgagacct ggtttctatt acaggctgta
    16981 tcaactcggc tgtattattg tcgtgtgtgt taaatgatgc ggttggtttg gtttctcaaa
    17041 atccacattt acaacgtcat gcctttgcta ctttgactgc tgctgattgg gaggaaagaa
    17101 cctctctctc ttataaatct caattgacag ctcgcaatag gctgaagaat ttaaatctca
    17161 tttatgagag aaatttcttg gccagccgcc gtatttttac cctggtaaat ggtcacgata
    17221 ttgttatatc catcaaaaaa cttctagctg gcaaacagga cgacggcttt agtccatata
    17281 actccaaaaa acgcgaaatt ctcagtttgg cggaaagggc caaatgtgat tggcggaaag
    17341 ggccaaatgg attggataaa gggtttcaga gcggtttcga gggtgatgcc cccctattcc
    17401 caagtatgac atccggaagt gtcgaaaggg aatattcgga tgtgtcgaat tggaatttta
    17461 agaaaggtcg tagtgggaaa ttcataagtg tcgattgggg aagttcagaa ttgccgaatc
    17521 ggcagattat ttattatata aattacaaag gggtttataa ctacaactac atgactgttg
    17581 aacccaacaa accacgttct tccgaacaag atcatgtggt agtggttgat gatgttgatt
    17641 tgatttatcc aaaacttttt aatgtgcaaa tgcgtcaaca ggcggatttg atttttacaa
    17701 aacatctctc aaaaagttcc tgggctttga ggcaggaaat cttggatgag atccagggac
    17761 agaaaaaaga agttactagc ccccttggtc ttttgattaa cctatgccgc tgcgcttcgc
    17821 aaggtaactt tactgctctt gtcgccccac aggttagatt ccagcgtgag caggatcaga
    17881 aattacaggc ttatcttgaa aagcagaata accaaaaaca ggactcatta gatgccatgg
    17941 atgtcgagct tgcccgcaat aatttaagaa agttctactc caaaagataa aatttaattt
    18001 aaaaaaatat ttttgatgaa tttttgaaag acgaataaat ctattacttc gaagcggatg
    18061 taacggggcg ttacagtcat cggcaaagga cagatatgat ggtgtacatg actaattagc
    18121 ctctgatatg caacaacagt gctttatgaa gctcttttta ataacttata aggatttaaa
    18181 aatggaattt gcaaaacaaa gatatatcat caaacctggt gcgatccatg ttgcacagca
    18241 atttgatctc aaagtcacaa caaaacagtc acctttcagt gacggttacg atatggctcg
    18301 tgctgctttg gctttggggg ataaggtcaa tcaaaagttg actgaggatg acgaacagtt
    18361 ccaacagtgg gaagagttta aagcacatga attgctgctc aagcaacatc tacaaaagga
    18421 gacaggtcga aagcatcact tggtttgggg ggagtatgaa attctttctt ctgagaacga
    18481 ccggtttaaa aatattggca gcctgacctc ttccggtgat gatggttttt ttatgcatac
    18541 caagcaaggt atccgtatgt gggagggcaa taacaacaag aagaacggcc cacgttggcc
    18601 gggaatcaga tatggtctga cactcagtaa cgagttgttt ggtatggcta tgtccgataa
    18661 tccttatgcc caaagtcggt tattgcagat tgaaaaacag atgtccgaaa ttgagaaatt
    18721 ttttgagact gtgaaaaaac aggttcatgc tcagattgat agtttggccg ctgcaggtat
    18781 gaagataacc ttgattcaga acaataaccc tgttcatatc agattgaatg tattgcgggc
    18841 ttatggtttc aaactggtta aattgttgcg ggattacgat agttttgtat gtgccgttaa
    18901 gacacttaat atcaaaggca tgatggctaa taaacaatgt aatgacacgt tgtataatgg
    18961 cggtcggatg atgagaaagc tactgaatga tttatatatt gcggtaatgg agaccagggc
    19021 tatcaagagc attcgacgtg acagtctgct tgacccagag cagttgtcga agttgaaatc
    19081 tgctgttgag caagagattc tgcctagaat tccattgtct gtttgggtgt atgaatcaca
    19141 accctctttg gtttatatcc agagaaaaat gaatcaggag gatctcaata agttggttga
    19201 tattgttcgt gacaatggat tatcgggcta gttgtgaaat tggaacgtgg ggttacaatc
    19261 gagttttaag atggtattga ttggcccttg tttgtatgga tttttttatg atgtgatatg
    19321 aatactatga atactcaaat atcaggtatg aaaaaatact taaaattttg ggaatacaaa
    19381 aggcttttaa gggtaagggg gattgaggat ttagaaaaag aaattaaatt gaaattggtt
    19441 gatttcgagc tattaattga tgaagctcag tcgtttcatg aagcatgtca gggccttaat
    19501 ctcatatatc ctatagtaag agaacatctg aaattgagta ataaaagttt ggctggtttg
    19561 gatttaaaaa aatactatat tccgaatatg atttttttca aaaatgttgt ttcttccagt
    19621 ggccgtatgt cgagaaagaa gttttttaaa tgggccgata ttagtacggc gttggatgat
    19681 acgaatgcca gccttgatga acggttatta catatgaagg tttattttga ttgcagacag
    19741 tatttttgcc gaggtaggca gattgccgga gacttggcag aattgttttc gatgaaagaa
    19801 atttttgatg aaattttaag aattgaaaat ttggatcgca aaaatcttcc tagtaatatc
    19861 atggttaagt aagatattta atagccttaa aaatcatttg ccatcacttt tgtgatggtt
    19921 tttttgtgac tgtaacgccc cgttacagta gtggatgccg ccggcggatt catcggcttt
    19981 cgtcaacttc ggttctgtgc cgatgactgt aacgccccgt tacagtagtg gatgccgccg
    20041 gcggattcat cggctttcgt caacttcggt tctgtgccga tgactgtccg tgggcatttt
    20101 agctagatat agcataaggt ttaatttgta atagttgcta gttgacaaga ggaatttatt
    20161 tagttatgat aatagaaccc ttaatctcat attgggttat tttaccctgt cttttctgtc
    20221 atatttttaa atcctttttt tggagaaaaa tatgtcagtt caactttttg ttcgtggcaa
    20281 tctcggtgct gaatttaatc tgcgtcctgt agcaagccct aaaaacgatg aagcctcaat
    20341 cgtactgaat ttttcagttg cctcacctaa tttcaaacgc aatgccgaag gtaaatcaga
    20401 agtggtttca acggagtgga tttcttgcga atactggaat cgtgatgctg cccatctcca
    20461 taaaatatta caggtcggta tgccggtttt cgttaccggt gaagaacgtt acgagtctta
    20521 taccaatcgt gacggtattg aagttcgcgt tcgtaaattg cgtgttcaag atgtattcat
    20581 tgttccaact gagcgtgtag aagctatcaa gttgcgtcct aaacgtgagc agtctcagga
    20641 caataatcac gttgaagacg ctgatgatga catcccattg taaatatatg gccacgaaga
    20701 atacttcctt ttttcgtgaa atgaactgtt ggcttgctgc attatgggct gactttaagg
    20761 atatgttcat tgttaccctt atttatggtg gcttaattgc tttggcaatt tgggcttttt
    20821 tgactatcgc atcttggctt gttttgcagc tgaagcaaag cttgggattt taaaatgcgt
    20881 ttgtttttat gtgaaaaacc atctcaagct aaggacatcg gtaaagtgct tggtgttctt
    20941 ggcgggaggc atgatggtta ttttcagaat ggagacacta ttgttacctg ggcatttggt
    21001 catatcctga cacaggccgc gccagatgct tatggcgaac aatatgctga ctttggaaat
    21061 atctccgcat tgcccattct ccctgaagtt tggcagatgc aggtaccgcc gaaggtatcg
    21121 aagcagttta aagtcattaa aagcttgttg gccaaagcag atgaagtagt gattgctact
    21181 gatgcagacc gtgaagggga agttattgcc cgtgaaatat tggactactg tggctaccgt
    21241 aaaaagattt cccgattttg gacgagtggc ctagataccc aatcggtaaa aaaagcctta
    21301 tcttcaataa tgccgggtaa ggataaagaa aacctatatc aggccggttt ggcgcgcggc
    21361 cgcgccgact ggctggtagg tatgaacctg agtcgtgcct acacagctgc ttattctgcc
    21421 ggatatggcc gagaacatat tttgtcgatc ggacggattc aaacaccgac tttaaagctt
    21481 gtagtccaaa gggataaaga gatcgatact ttcgtgcctt acaactacta caccctttca
    21541 gtagattttg agacagcaga caagtcagtg tttactacac tgtggcaggt tcctgaaaac
    21601 tggcgtaata ctgacggata ctgtaccaac cttgaggctg taaaggcact tgtcaaacag
    21661 cttgagaaat ctacaggcaa ggttattacc tcaaataaag agcgtaaaag caccccgccc
    21721 ccgttaccgt acagcctttc ggctttgcaa aaagaagccg gtaaaaaact tggcctatcg
    21781 gcttccaaag tgctcaaaat tgcacaaacc ctctacgaaa aatacaagat cacgtcatat
    21841 ccccgcaccc cttgcagata cttgccaaaa tcgcagatga gtgaagtgaa agatgtattt
    21901 gccgcgatac agactatcga taaatccatg ctttcagtga ttgaaaaagc tgatcctgaa
    21961 cgtcaatcaa gggtatggaa cgataaccag gtaaacaaac attcccatca tgcgattatt
    22021 ccgaccaagg ccagtaattt tgatttgtca gtcctggata agaatgaatt aacggtgtac
    22081 cgaatgatac gagaccgtta tatcgcacag ttttatccag attttgaata tgactctact
    22141 gtagttgagg ttgaggcatg ttcacatttg tttaaagcct ccagccaatc accggttatt
    22201 tccggttgga aggttctttt gggcaaggac gtgtttgaag gcgatcaaat cgatgaaggt
    22261 cctgctctac cacatctgaa agtcaacgat gaggtcgaca cgctttcctt caatcccgaa
    22321 actaaaaaga ccacgccgcc accgcgcttt acagaggctt ctctactcga tgagatgcag
    22381 actttgaagg actttttgaa gaatgtagaa gacgaacaga tcagaaaaat tctcaaaagt
    22441 acggaaggct tgggaacgga agccactcgc gctaccatta tcgatcgttt gtttgaaatg
    22501 ggttatatgg aaaagaaacg gagcaaaatc tatgcgacag aaaaaggccg taatctgatt
    22561 gcccgtattc ccactatgat tgccgatccg ataacgacag ccaaatggga gctggctttg
    22621 gctgggatag aagccggcaa attaacttta gctgaattta tggcttatca gcagaaagtg
    22681 atcacagagc tggttgggca ggcgaaaaag gatgctgtag gaaaagctcg tccgccaaaa
    22741 cagacggatt cagctggtac aaaaaaacag gcggtcgctc gaaatgaaga cgatgtctgt
    22801 ccgacctgta aggaaggccg tctgagacag agaggattca aggaaaaccc tgataagcgg
    22861 tattgggggt gtagtaggtt tcccgaatgt aaacacttcg aatgggtcaa ataaaaaagg
    22921 actttgaaat gaagaagctg gtaaaaaaat tatccgattc aatattcgga aatacttgta
    22981 tagagtatgt gctagatgaa aaccataatt acaaggagca atacaacgct aataaagatt
    23041 tattcattta catcaatggc attcaagtag ggagtatttc acctggcgaa ctgcaagaat
    23101 tgcaatatag agtgttttat tctagtagcc tctggtggag tatctttaaa tctatactag
    23161 gatttttatt gaaacccgca ataattgcgc ttactgccct gccgctgggg gtcttgcttt
    23221 tactgtggtt gaacaccgac aatcctgaag cgtttcatcg attattcatg atgttacagg
    23281 atccggcaca ttttgcaaaa gcaatatccg gactttttcg tctgttttat tgtatatcat
    23341 tctttggcac ttttgttaaa ttggttttta accaagattc aattttttca atttttaata
    23401 aagaactttc taagaatata cagagtaaca aaacgtgctc gatggatatt gtggctgtca
    23461 gagattgagg aagctcaaag atgttgccct ctaccagcat actgggaagt ttgcatatca
    23521 atactgagtg ttgcttaaaa tttgattgga gtcattggtt tggaatttca gatggataga
    23581 agaaattttt tgaaaaagtt tgtcggcgtt ggcgccctat atacgaccgg tgctgccggt
    23641 ttgttattgc ctaaagatgt agtagcggca accagtatgg ctgatttttg gtcacgagac
    23701 cgtacaatca attgcaaacg ggcagatact ggcgagaaac atgaaatccg tttttttcag
    23761 caacaaaatg gatatgatct tgatgcttac cgcaatgcct gttggctgat gcgtgatgcg
    23821 aaagatggaa atgccatggt tcaaattgac gtagggcttt taaacctgat gtatgcaatg
    23881 caggagtggg cacggcaatc aggacggtca aaccctgtga ttacgatcaa tagtgcatat
    23941 agaacgccaa gacgaaatgc gactattgag ggtgctgcta gaaactcact tcatatgcgt
    24001 ggtaaagcag ttgactttac tatgaggggg gtgggcattg gcgagcttga gcaaatggct
    24061 aaatactaca atgtgggcgg tattggtatc tataatagtt ttgtccattt ggataccggc
    24121 agggttcggc attggcgcgg ttaaagattt tacgaaaaaa tattgacata cgaaattatc
    24181 gggaatatat tgtaatcgtt tttgtagttt gtgacttttc ttaaaagtct cgtcatttag
    24241 ccgttcatga atgagattcg attcgtcgaa tttacggtac ttctttttca gccagccttt
    24301 aaggcttcta agtcagttcg gctaccgtga aagccgcaac ttaacccttt ggggatatac
    24361 tgtccccttt gggattgata tatcccctgt ttttttggag atatatcatg actcaatcta
    24421 atcaaaacta ctttaacctg actgcagatg gtatcggttt tttaaaccgg ccacgtgaag
    24481 ttcaaggaaa gagaaaatct tccaactatt atgcttgtac gatccaagcc tcccgaggtg
    24541 ataatgggga gaaaacccgt ttcgatcttc gtatcgtcgg aggtcaagcg aaagagctct
    24601 ttgctcgcct tttggaagaa tttccgcaat tgctgtctaa aaattaccgt gaacgcccga
    24661 ctgtcgtggt cggtttccgt gccggtgata ttcaaccttt aaaatttgaa actaacaatc
    24721 gtgagacggg cgaagtttta gaaactttaa gtatcgacgg tcggttgttg aaattcaaat
    24781 ttattaaagt ggatggcgat atgtggtatc aatcatcctc tcaagatgaa acccaaaata
    24841 atcctgatgc ttcttagaaa gctattgaat attttgatta tttaagaatg gtaggaagct
    24901 ttcgggcttc cccgttctta gttttccagc tcattgcttg gaaaattaag cctctgtcga
    24961 aacggcggtt taattttcca agcaactcct gaattatagt gaagcaaagg gtaagtcccc
    25021 tcaaagactt ggctttcagc aatattgttt ttttatcgat gctcatatct attgtgagca
    25081 tcagtaaaaa aatacagttc tttaacaatc cgaatttttt atttttgttg cttttgaagc
    25141 aactgtttga aggctgccct ttgggcagct tacctgaatg cccctcccta ccataggtca
    25201 ttcaggtaag cttccccggt tgacagaccg tttcttctgt ctgcgtatag ccgtataacc
    25261 agtgcgcgca attattcgta attgttacgg ttttattact atccagacca ttatctgatt
    25321 acttcgtcgg tgcggactag gtaatcatta gccagggtaa tccccatttg aacctgcttt
    25381 gcaggttctc taagacgttt tgatatttgc agaacggctc agagaatctg tatttcaaga
    25441 gttttataca gctgacagac tgttttttct gtcattgcat agccgttgat tgcaagcctg
    25501 tttcaggtac ggtacagctg cttttttctg aagcagattt cttttaaccc atcggggtac
    25561 acttccccga atgggcatgg tgtacccttt attttggagt attaccatga atctaaatct
    25621 atcttttcac cgttcaggtg tcatccttcc cccaaattta ctaccggaaa gaatacgtat
    25681 cggtgacatc ctcacccacc gacatactca tcggaaagtt gtcgtctcat gcatacaaga
    25741 acatcatttg ttacttgttg atgcggatgg ccgtatttcg aaaatacgaa cccaaaaggc
    25801 ggtcaatcgg tattgtagga gcgtcaacga tgttcatagc cataaaaacg cttccgtagc
    25861 gttgaatatg gctattcggg cattggataa cgacaaacgt atttttacaa gattgggact
    25921 gcgtatgtcc caaaaagtat atttggataa gatttacagt gctattaagc gctaatttgt
    25981 tttttcccaa ccacgggata tctgcccgcg gccggtatcc cttttctttt ggagtgatac
    26041 catgtccatc aatctttccg gcaccttgac ggtgcaagtc atacagggtc gccgaggccc
    26101 tttcaaaaca gctaaattag ctacatcaat tggaacattt gatgtaaaaa aaccaatatt
    26161 gaaacagttt gagcctggtg aataccaagg aaacttcatt attgaacagc tcaaagttaa
    26221 ggcttatgag tggggtggtg gcgccacctc ttacttagat gcagctcttg actggcaaca
    26281 tctggcggaa tttgcgcagg aattttcttc cgggtcggat tctccgcaag atgatccatc
    26341 cgatccgttc gatccggttc ctgaaaacgg catccggttg cctgaatacg cggaaactgc
    26401 ggatcaggtc ggcgaaatga tcgcccaaaa ccgggagcat atcagtttgg gtgagtatat
    26461 cctttcagac cgtgacgagc tgaacatagc ccgttcaatg atgaaggagg cagggtattc
    26521 ctatcggccc cagtcgcaga cttggtgtct gcaataggag gttggaatgc tttacctgac
    26581 ttcccgcaat gatttgatgg ccgatgcctt cttcatttgg aaccgacagt tgatgtttgc
    26641 ttctctgcac ggccgcgatg cagacatgtt gtccttccaa gctcaactgc aagtaagtaa
    26701 tgaacggctt ggttttagac ggccggagga ggttttaagc tgtccgcgcc tgacgactgc
    26761 cgagcattgc ttgaatctga gcaaatacat gacgaaatat cagactctta attatggttc
    26821 ggtcacgcac atgtttttgt attcggatat tctgattcaa cctaacttcg atagtaaaac
    26881 aggttgggtt atgttggatg atgttaccgc agacttggac aaggcagctt ggcaactggt
    26941 gcggcaactc tccgatatac cgttattgga acattggcag aatgctgttt tatcggtact
    27001 tgaggctgat aaatccatca tgcggtttac tcctcgtata gatgaagagt acgcagtagt
    27061 tggagttcaa gcggtacgag ttgatatccc ggaagatttt gatgtgcgtt tgaccgctat
    27121 gctacaaagc ggccgcttac atacttaatc tgaagcccct agcgggcttt ttcagacggc
    27181 cttgcaaagg ctctctgaaa aagtctgtta cataagagtt ttacacagat gacggactgt
    27241 atattccgtt gatggatatt gctgctgagg ttatccggcc gtattcgtat ggttgcggca
    27301 tttttcaatt ttaccaaccc tgtggggata tacgtcccat ggggaggcat attcccaaac
    27361 ttaggaggat tttatgccta ctctatctga aaagcatgag gtactgtccg gcttgaaccg
    27421 tcaggatgca gctgctatag tagccttaat cgatttgaaa acggaggacg acatggaaaa
    27481 ggttttgaac aaactggatt tcatgaccca gaacttcgat gcccgactgg acgcttttga
    27541 aaaaagtgta gatgcccgca tcagctcatt tgaaaaaagt gtagatgccc gtatcagctc
    27601 atttgaaaaa agtgtagatg cacggtttag ctcatttgaa aatagtgtag atgcccgcat
    27661 cagctcattt gaaaatagcg tagatgcacg gtttagcgca atcgatacta aaatcaatgc
    27721 aatttattgg tttatcggta tcacgactac cgttgccgtt gctgccgcga agttcctgta
    27781 acattaaacc cgttccgtta aacggagctt tttatctgta accttcaatc ccaccatgtt
    27841 ccggcatccg gtgggatttt tatcacttct cactcaaccc tatggggata tacataccta
    27901 tggagatcgt atatctccgt tactttggag catgtgatgt tttctacaaa ataccctttc
    27961 atatcttgtt ttgtacggta atcagccgta caatctagaa ataatttagg agtatgttca
    28021 tgactgccac atatgccgat tgccgttttg aagcaagaat cacttcagat acccgtgctt
    28081 tacttaaaca cgctgcggaa cttcaaggac gcagtatgtc cgaatttgtc atcagtgctg
    28141 cccgcgaggc tgcccaaaaa gcagttgccg atgcggagat tctgatgctg tccgaacgtg
    28201 accaacttca gtttgcccaa gccctgctca atccgtccaa accgaatggg gcattgagca
    28261 gagcattgga aaaacatgac gaattgttcg ggagataaaa tgccgttacc ggacttccga
    28321 atccgcctcc ttgattctgc cgatgaccgc aatgcatttg agcgcccttc cgaaccttta
    28381 aaccgctatc tgcaaaaaca ggtttcgcaa gacgtaaaac ggaaagtcgc tacttgtttt
    28441 gtcgcggtta catccgacgg tcaaattctt ggctactata cattggctgc aacaggagtt
    28501 gccctaagta atatacccgc cggttttcgt aagaagctgc ccagataccc tacggttccc
    28561 gcagtgctga tgggccgttt ggcggtagat ggaaaatatg gcgggtttgg actgggttct
    28621 ttacttttgg ctgatgcact caaacgtatc ggtaaagcag aaatcgccgc gtatgcgttg
    28681 attgtggagg caaaagacga agctgccatc ggcttttaca ggcatttcgg ctttacttct
    28741 ttcgaagatg ccaccaagca tctatttttt cctttggcga atcttcgcta accccttttt
    28801 ccgcaccata cggtagcggt ttctaaccct gacggggcaa tcccccgtcg ccggggtttg
    28861 ctccgtcttt tttttaggag catacgatgt ataaccccgt atcaaaaata gacgaactcc
    28921 tcccggattt tgcagggctg gatcaaactg tgaccgagat gcaggaacgt cagaatcaaa
    28981 taactactga tttcaacttt atcatccgcc aagtggctga gactgcccaa aaaagtacgg
    29041 aagctgccgt attgccggag gaatctccgg aaactgaact gccggattcc gacatacccc
    29101 tgacacagtt tcttgccgag tttggggata gtctctttaa cgcagttact gagcaaaacc
    29161 aaccggtcta tcagggacaa acttatcctc tccttgaaga agtgctggat aacctggaac
    29221 gccctttgtt tgcggcgcag cgcgaagttg tgcgcgccgt attacagcaa ttggtggcag
    29281 aggacaaacc cgcagccatt attaatgccg aaatgggcac aggtaagacc atgatgtcgg
    29341 ttgcagcagc cgctgccgcg catcaagcag gtttgcaccg caccttggta ttgagtcctc
    29401 ctcatttggt gtacaagtgg agacgagaga tcctcaaaac ggtacccaat gcccgcgtat
    29461 ggattcttaa cggagccgat acattggcaa aactgttgca aatccgtgcc cttggccgca
    29521 aacccgaagt tccggagttt tttgtgatgg ggcgtgttcg gatgcgcatg ggctaccatt
    29581 ggaggccttc gtatgccgtt cgccgccgcc ggataccttc tgaaggtgct tctgagggtt
    29641 ttgccgtttc cgagtattac tgtcctaaat gtggctgtga aatcttggat gaggaaaaca
    29701 agccttataa cactgaaggt ggtgtgaaaa acgccctggc taaaaaccgc cgtttttgca
    29761 aaggcaatca tcatggcaaa ccgtgtaagg aaccgctctg gaccttgtgc cgcaaggaac
    29821 agagtgaaga cggggttgta agcacagtgt acgatcgcgt acttaaaacc atgaccaccc
    29881 tacccggcat cggtcctaaa accgcagcaa aaatcatcgg gatgtatggt gagcagtatc
    29941 tggctgaaat cttggaaaac aacattcagg cgttttccaa tttgatggat gaaaacggag
    30001 aatttttatt cgacgaccgt caggcggcgc gtatcgaccg agacttaggc aaagcagaat
    30061 tcagcctggg acgaggcggg tatcagccga ccgagttcgt caaacgctat ttaccgaaga
    30121 actttttcgg gctgttggta gtagatgaag gtcacgagta caagaactac ggtacggctc
    30181 aaggtcaagc catgggcgtt ttggcacgtt gctgtaataa gatcctgtgc ttaaccggta
    30241 cgctgatggg tggttatgcg gaagacctgt tctttctatt gtggcggctt tggccgcaaa
    30301 tgatgattga agacggtttc agttacaaca aagggaatac cttgggttcg gcaagcatgg
    30361 cgtttatgcg taagcatggc gttttgaagg atattgtccg acatcttgga actgaataca
    30421 gtaatggcgc attcagtagc tcaaaagcag agcgtaacag cgttcgcacc gccaaggcgc
    30481 cgggattttc ccctttgggc atcatgcgtt atgttttgcc tattaccgta tttcttaagc
    30541 tgcgtgattt gggagaaggc gttttacctg gctataaaga agttttccgg ccggttgaaa
    30601 tgactgaaga tcaacaggcg gtctataaaa atatggagag tgttttgagg cagcatttga
    30661 aggtggcatt aaggaatcgt gacaataccc ttaccggcgt tgttatgagc gccttgctgt
    30721 catggccgga ttgttgttac aaacctcaag atgtttactg gcgcagacaa aacaagattc
    30781 ttttccatac cgaaccccag ttcgatgaag aaaaaatctc accgaaagaa gctgacatgc
    30841 tggaagtcgt taaagacagt ctggctaaag gacgtaaatg tctggtctat actgtttata
    30901 ccgatacccg tgatacgaca ggccgtctga aagagatttt caaacggcac ggtattaagg
    30961 cggcggtaat gaaagcgacg gtcaaagccg atcaacgcga agactgggtt gaagaccaat
    31021 tggcttcagg cgtacaggtt gttatctgta atccggagct ggtcaaaacc ggtttggact
    31081 tgttggcatt tccaaccatc tactttttgc aaacaggata taacgtttat accctgatgc
    31141 aggctgcccg ccgttcttgg cgtattggtc agaaggaaga cgtggaagtg tatttcgccg
    31201 gttacagcga tacagcgcag caaatttgcc ttgaactgat ggggcaaaaa gttgccgtta
    31261 ctcagtcaac ctccggtgat atgcccgatt cgggtctgga cattctgaat caggctgaag
    31321 attcagttga agttcagttg gccaaacgct tcgttgaccg acaagattga tgcaattttt
    31381 gcatctaacc cgcgtttagt gggttttttc agacgacctc gtgttgaggt cttctgaaaa
    31441 agcccgtaag gtggaatttc acaggctggc agaccttatc tgcccgcgca ctgccgttac
    31501 ccattgcgca cgtccttcgg gacggacggc tctttttcaa ctttctttaa ccctgtgggg
    31561 atataccgta tccccacagg gcatcggtat atctcccttt ttcatggaga taccgatgtg
    31621 aaatttttta tattcatcaa acccgacctt ccttttcaca aggaaagcat tgaatcttgg
    31681 atcaaattgc tggaagatat aggtaaaaca gccttgttgg caatgctgcc gttgttttgg
    31741 ctcaattcat actctttgac cgaacgtgct tccggcgtag ccgtattggc tttggttgcc
    31801 tactttgtgc aagtatatgc tgacaaacta cggaaatacc gtgattcatt aaacagaaag
    31861 gatacgtcat gctgataggc ggtctgctat ttattctctt cctggtgctt gctttcggct
    31921 attggattac caagcagctt gaaacttcgc gtacgtcgga agaacacaaa aaccgttaac
    31981 gcggttcatt ctatgtagct caagtattta tcttttactt ggataaatat gaatcaaaga
    32041 ttgtcaaaca ggaacccaag gaaagggaaa aaccatgatc atgacctaca gcttactatt
    32101 tttgattgcc gtcatgttgt ttggcatatt taaactggta aagtttggcg agtctcagca
    32161 agacactaat acacatgacc ggtcaagtta attggtattc ccatctttta aatgctttgt
    32221 tgctatttaa taccaaagca ttttaaatag aacgctgata cgttcctttt tagataaatc
    32281 cggcttgaag ccgcaatccc gctgttccgt acgtctgtac ggcagtggga tttttcttgg
    32341 aagtcatgcg gttacgtttt ttttaatcgt atgcgtgtat ttttaaccat ttgcaggagg
    32401 aaaacctatt tttgtatcgg agaagaaatg agatatagca aaaacaaaga ttatcaattt
    32461 tttatacgcc aactggtatc ggggggagaa tggatgttcc tccccaaaaa cggtcgtaag
    32521 cacagcgcat tgaagcacct accgaccgat cgaaaaattc cgatacccgg ctcgccggga
    32581 caggatccgc gtggattgct gaattttaaa acaatggtac gtcatataga acgtggcggt
    32641 accttcgatt aaactttgat ttaaccctgc ggggactgtc ccgcacggca tgtctccgtt
    32701 ttttatttga atggagacca atatggaaac tcaaaaccat ctgatgcacc cccgtgtcgc
    32761 gcataaccac cgtgacaacg gatacttccc caccgaccag gccacattgt cgcgtatagc
    32821 cgcttgcctt gatattccgg catctgccgg aggtttgtta cgcatatttg atccttgctg
    32881 cggaacagga gaagccctgg cttctttagc ttctactttg caatcggaaa aaataagttg
    32941 tcaaacttac gggattgaac tggagaaaga ccgtgcccaa gaggcaatga gccgactgac
    33001 cagtgtcatc aaatccgatg tagaaaactg caccatgcag ggttcggcag tcggcctcct
    33061 gtttctgaat cctccttacg gctttgccgc caaagacaat ctgtctaatc aaagaaccaa
    33121 acggcttgaa gaacttttct tcgcacgtac cttcggtact ttgcagcccg gcggagtgtt
    33181 ggtaatgatt gttccggaag ccgccctgac agagcatcta accggcgaaa tcgctaccca
    33241 ctgtacggat gtccgtatga taagggctgc ggcggatacc tacaaacagt tggttatatt
    33301 cggcatccga ccgaagaaca aagcctctat cagcaaaaaa caggcggatg cccaacaaag
    33361 gctcctgctt gattttgaaa atgcccctga gactttgccg gccggtatgc ctgcatactg
    33421 tgtgcctatt gcacccaata aggtctttcg ccccagaagc tttaaattgg agcaggatat
    33481 attggaacgt gaactgggac agcagcaatc ccgcacgtta tggccatcat tttcgcaata
    33541 tttcggcacc agtgccgtta tagccgaaaa acgccggcct ttatgtgctc tgggtcagtg
    33601 gcatgctgct ttagcattag ccgcagggca agttaatggc gttgtcacct caaccagtgg
    33661 ccgaaagctg ctggttaaag gttcaaccca taaaaccaaa gtggaaacca aatcggaaga
    33721 gtacggcaag aaggacaggc ttgtggtcac gattacttca actgaccggt tttctccaag
    33781 catacgcgca attgatttga ccgaaggatc gtcggatttt ggacgagtcc tgacaatcaa
    33841 atagattggc ctattcaatt tttaaaccct gccgggtact tcccgcaggg gtgtatccgg
    33901 tttttttaag gatacgccta atggaacaac ttgattcggc agctccggcc gcatgtgata
    33961 ccccctctct gtttgacctg ggccgagtgt ctatgacggc aacagtagat taccgggcaa
    34021 aagagcacct gcctgcacaa ctgtatgaga acagtcttta tgcccaaata ttgcttgagg
    34081 cccaccgcca tggcatatgg ggtgatattc ctcctgagga tgccaagccg aatcagctgg
    34141 cactcaagcc aggtgaagaa ggcagaataa tgtcttctta caagattggg gaccaaaaaa
    34201 tttgggttgt tacagaatgg gacagaagcc ttaccacact cctgttccca gaagattact
    34261 gaccttgtaa tctttttata tatctcacct gccttcgggc aggtattttc agacggcttt
    34321 agtaaggccg tctgaaaata cctgtcttta caggctggca gaccttatct gtcttgtgtc
    34381 ctgccgttaa acatgacacg catccttatg gatgtacggc attttctttt atcaaccccg
    34441 tggggaacac aatccctacg gactgtgttc tccgttttct ttttaaggag caacacaatg
    34501 caaaacttta ttgtatggat gaatcccgat cgggaagaat cggcagctgc ggcagctaaa
    34561 ttgaacggtg cagttttgac cgctcacacg gatggtgtca tggccgctat tagacgtgaa
    34621 ctgaaaagat ttggattagg ggctgatttg gccgtctttg cccaaacctg tgaagagttt
    34681 gccaaacagg ctaatgccga catggtttgc atcagcagta gcaatggtga tgccgttgca
    34741 gccgtcactt accttgagga cggatacgcc ggtaaaggcg tatccgccgt tgccgacatg
    34801 cgctatctga tgcctgccta tcggcagtat ttcaaccgtc ctgacaaaat ggttattttt
    34861 cagggggcat tgcagtattt atcagatgaa gacattgcct ggattttccg ccgccacatc
    34921 aatgccatca tcgagtggca gtcgaatgaa gggctggggc tgtacgagtt cggtttcagt
    34981 ctatctgatg ttctgactga tggctgccgc catgtgctgc gcgaagtcgt caccggctgg
    35041 ctgaaccgcg aaatcctgcc cgagctgtac aatgtgtagg cataaccgcc gtgcttcttt
    35101 tccaactggg taaatgccca ttacctgcgg tcttttggcc gcaggggcag aaactctact
    35161 ggagatagtt atgtctatca aagttgacaa catccttcta atagaaggaa ccaaagaatc
    35221 ggttgccgaa gcggcgaaac tgattttatt tcgctccccc gttcatcctg agcattgggc
    35281 ttttgatttc aatcggattt tgcctttacc cgaaaatatc acgtctgacc ttgccaaaca
    35341 gagaatcgag gcatggggca gcagtttttc cccatatccc accgaaacct ttgttacaca
    35401 aagagagggt tttattgaaa tctgcttttt aaccggattg gcaccgcctt tcggtattta
    35461 tcgcaaactc gcggaaatct tcgccaactt ggatgtatgt tttacagcga aatacatcaa
    35521 ccagtacgga agtttcggcg gacgctacga ataccgtaac agcgtgcttt accactgtct
    35581 ttgcgagatg gccgacatcc gtaaattcgg tatcgccgaa ttcgatattt ggtacgaagg
    35641 cttgtccgat agacttgaaa gcgacgggta tttggtagaa gattagacta tgttacacgg
    35701 taggcagtcc gtcaaaccgc ccgcataccg ccgttaacca aatgcacatc cgtttcggat
    35761 gcacggctct tttatcaacc ctgaagggga tgtattcccc gtcatcggtg tggtggtctg
    35821 gagtaacgga tatagtgagg agatgaagaa tcttgccatg gcaggattgg tgctcctgtc
    35881 gtttgaaatg ctgtatgttt cattccttat ccgcattcac atctctaaac tggaggacaa
    35941 ataatggact tcgttaccgg aacttgggtc atggccgcag tcggcctggt tcttgttatt
    36001 gttgcgagaa tctatcttaa aacccccaaa aaataaccgt gcttaagcac atttttcatc
    36061 aagccggcgg aaccgtcgtt ccgcttccca aaggcaaagt taccgtttcg gtttctttgc
    36121 cttttatcta aagcacctgt cgtctgaaat gtgcgccggt ttccggtggc ggctttttta
    36181 tcaacccggg cgcaagccta tcaacctgcg ggtttcctta tccgcaggga tggggattcc
    36241 tgctcaggag actactatgc ccaactggtg cagcaacagc cttcattttc aaggcaatcc
    36301 tcaacgtatc gccgaagtgg cggagcaaat tctgatcaaa accgacaagg ggtatcaatt
    36361 cagctttcag catattgtac ccatgccgga ggagctgatt cttcctgaag tgcctgatag
    36421 ttacatgcag cagacctggt ttcaaattgc tcaacaacct gaaaagaagt tgatcaattt
    36481 ggaacgggaa atgctggaac agcgcgataa atccttttgg tccgttcaat atagattgcc
    36541 ggatgaactt ttctctgaat taaagctata tttttcggaa aaatttgatt gggacaatct
    36601 gactgttggt gaagtatttc tccattttga agctaactct gaagtcgcat ctcgtttccg
    36661 ttacgatgaa ccatttgcca agcgtattgc ggaaaacatc cgccaatacg gtcatccgaa
    36721 ttggtatgac tggcggttag ctaactgggg ctgcaaatgg gacgtaaacc ccgactgcac
    36781 ttttgtgaca gttggggaaa gcggcatccg gatcagctgt gacactgcct ggggtcctcc
    36841 ggaagggatt tatagagaac tcgccaaacg ttttcctgac gtagaatttg aagccaaata
    36901 cctggaagaa ggaatgtggt ttgcaggaac ctatgaaggt catgaaggag cattgttcga
    36961 ttatccgtgc acagatgatg gagtccgtga cttcgctacc gagcactttg gatgcgaata
    37021 cgatgacgaa gattaaagaa cttagatttc gttagacgta ttgccaactc accctgcggg
    37081 gacaacccgc agggttgtcc cccgcttttt tgtggagatt agaaatgatt gaaaatcaga
    37141 ttgacaaaga gatcactcag gcttcatgcg aaggccgatt catcctaaaa caggaaaacg
    37201 gcaaacgttt cctttacctt aacctgcccg aaggtagtga cgaattaaat accatttggc
    37261 agacggacga gtatgacttt accgtacccg acttagaagt cagcatcgat gttgaatccc
    37321 ttcacactgc tgttagactt ttgaacgaaa accagggcat tttgcacggc atcagcacaa
    37381 aatgcagtgc gtattcgttc ggtttcgaag gaaagttgag atacgaaaga ttggatgtaa
    37441 agcctttccc tatcaaatcg ttctcttatt atttggagtt ttataacgac tggacgggga
    37501 cgttgtatga actggactta tcagcgttcc tggatgagtt tttcggagag tgtgatcctg
    37561 aatccaagct cgatgcctgt ttgaagtagc atttgtgcct caaataatct ttttcaaaac
    37621 caacggccgc taatgcggcc gtttttttgt gcccatactc tgatattttt ggggtctctg
    37681 cccccaatcc cccggtctcg ccgccgacag ctcgggaaca ggaattacct ccggcctacg
    37741 cctgctccct cacttaagcg tgtcatcgct gccagtcagt aaatgggtat tcataaacgc
    37801 ccttgcaggt cgaaggggtt gggtattgac cctgtaagga aatttcctta caataaacag
    37861 aattctttaa tttaagcagt ttttaacaat atcaataatt tcccaagccg ttaagtagaa
    37921 gatgtgcgta actaggacat tgaagtcgca agtagcaaca gtaaatttga atgaaggcag
    37981 ggcaaagaga ccatgaagat tatttttgat gcagtgtcaa ctatgaccag caaaaaccaa
    38041 acaaccattc ccgagtcagt acgcaaggcg ttggggctgg aaaaacagga caaaatccgt
    38101 ttttccgtct taggcggcgg acaggtattg ctagaaaaaa acaatactga ggaagaagaa
    38161 tttgagcatg atccagttgt tggaaaattc ctgcattttt tggaaaattc tatgacaaaa
    38221 aatccgaatt ccatcaaacc cacatctcaa tcacgcttta accgccttcg ccaactgact
    38281 tgggaaacag atatgaaaga ctaatatgcc tgattcccct atgcagataa acggatatac
    38341 catttacgaa cacgaatgct ttacgcagca attagacaga ctggccacta cgatagaaag
    38401 ccagaaacga tccgaaaaat ccagtagatc cgaagaggcc aaactgctgg aacgaatctt
    38461 aggcgaaata gaaaatattg cccttaatcc ggatagcccg gaatatcggc ttggcaagac
    38521 actcggcccg gaatacaaat tttggcgacg tgccaagttt atgggtagat accgattgtt
    38581 tttccgttac gactcaaaca gtaaaatcat cattctcgca tgggtaaacg actcgggtac
    38641 gaaaagaact tatggcggca aaaatgatgc atatactgtg ttcaaaaaaa tgctggggaa
    38701 cggaaatcct ccttcagatt ggactgcact gcttgaagta gtatcaaagg aatagagcaa
    38761 aagaaaacag cccttagaag attctcatgg tcaaaataca gtattagact gaatgttaca
    38821 acattgtaat aataattgct gttatctata atgttgccca tgtccgtata tgagccggac
    38881 aaatctaaaa gggggaagaa aaattgccct ttggcggcaa acaggatata taggatttta
    38941 ttgttgtctt attctgatga tgcttttctt cccaaaggat tttaatcgtt tgaaaattat
    39001 tgcttatacg agtccggatg gtgatgaatg ggtaactgcg ctggaagcaa agctaaaaca
    39061 ccgggaagga atgatctact acagccgtca tcgtcccggt acaagaaaga aaatggtttt
    39121 gaccaggagg aaggcgacca atttttttcg ctattacagt gaagccgact ccggtggtgc
    39181 ttccgcccct gaatcgctaa cccacttgct ttgcaaacaa gtgctgaacg aactatccaa
    39241 cttacccggc ggcctgacga ctgttctaaa ttgcacagaa cacgccgagc aacaaccgcc
    39301 tgtcactatt aggctgaacc gtgcattatc cgaatatcgg atagatatag atggcaaaac
    39361 tttttatatt gatgttttgc ttgaatttga tcagccgggt aacacttccc tgctcaggca
    39421 cgagataagg tggcagagaa aattggcggt tgagatttgg catacatcac ggttggcttc
    39481 caatgcgcct aaatgtttag ccttatcgaa aataggtatc ccggtggttc agatacgggc
    39541 agataagggt tcatttttgt atattgatga agacgagctg ctcaattatg ataatgaaga
    39601 aatcaaaaac cgcataaatc gccatgtaga gaaacttcgg aacacctttc ggaaacaaat
    39661 tctctgcacc ctactccgta atcctttgag tgcggatttt cagacggcct tgatgttgca
    39721 taatcaaatt aaggccgatg aacaacaagc agagcaaata caggaaaaat ttgaggcgct
    39781 gaggaataaa catgttttat tggaagctga atattcggct ttggccgccc aatacgcagc
    39841 cttattagaa catcagaatt tccaagctca ctccgggaaa agggaaattc cgaaaaagca
    39901 tgggattctg cagcgcatgg ccagttggtt taaatcataa accactgttt agaacaaacc
    39961 ctaagtttca ataagatgag gaaaatattg caatgaaaaa aattatgttc ggattattag
    40021 cagctatggc catttcaaat gcccaagctg aaggtaattt cttgaatctt gcttcaaagc
    40081 cggccaaaat tgacggggta ttggaagctt ctttagacaa accctatccc aagtttgtac
    40141 aagattggat gaaagacagt aaattggcgg gacttggccg tatcatccaa ggcaagattt
    40201 ttgaaagtac gggaactatt gaacgaaaat tctatgcaag atccgaatcc gaacatgaaa
    40261 aaacggttga ggatgcagta ggaactttca aagcatattg cgaacatcat caggggacgg
    40321 ttgaaaccct gacttccgat attttctgta tcaacggcag acaagtccag tggatgggat
    40381 ggtggtacag agaaaactat acgaattttg cattctctga tggatataca ggggaacaag
    40441 cctaccgcag caaagttacg gatgtacaac ccggtaaaaa aatctccact tcctatgggt
    40501 ctgccttggt cttagaaaaa ggtgagaatg gcttaattaa aattcagccg aacagaggca
    40561 gcgaacgttg gattacaagt gacagcatcc aagtaactta taaataatgg gatgtgtatc
    40621 atataagaaa gaggattggt agctgaaatg ctgccaatca catggagtgc aaattgaaaa
    40681 caaaatctaa aaaacttttg aggcttgtta tcttatttgt agcggcattc tccatctcta
    40741 cacctgcggt aatgtacttc agcacaggcg aagcgacatt aaatcttttt gttgaggggt
    40801 tttggataat tggattactt caagcactcg cttttaccac cattgtagac tattgcactg
    40861 aatctaaaca tacaaatatg ctcaataatc cagcgtgcaa cgatgaagat tgggataatg
    40921 atatgctcaa taatccagcg tgcaacgatg aagattggga taatgatatg ctcaataatc
    40981 cagcgtactc ttggtcaatg ttcaatattc accattcaga ttaaaaaagc aggagcctta
    41041 tactcctgct ttccgcagca aagttacgga tgtacaaccc ggtaaaaaaa tctccacttc
    41101 ctatgggcct gccttggtct tagaaaaagg tgagaatggc ttaattaaaa ttcagccaag
    41161 cagaggcaat gaacgttgga ttacaaatga tagcattcaa gtaacctata aataaaggag
    41221 aagtatcatg atcggattcg taaaatggta tagctatgag aaagattttg gctttatctt
    41281 tggtgaagat ggtcatgaat actttttccg taaatccgcc ggttcagata taccaggaca
    41341 gatagtgaat ttcgaaccat ttgagcatag tcgtgggctg cgagcgcgaa aggttagtaa
    41401 tatacataat cctgatcctc aacagatcca aaaattgaaa accttatgca aagactttca
    41461 gaaagttgaa aagcaaccca tgtctaaaaa atacaacttg ctttcggcag ctatattatc
    41521 gctgtgtttg actatgttca taacactggg ctttttctac atcttagccg gcttttttga
    41581 tactgatagg gagactaaca tagttttgct gtttgtcttc cctgcggtaa ctttttccgt
    41641 gctgacattc tttctgaata ggatattcag gaatatgaaa ccggggtctg acactgatgt
    41701 tgccactaat gatgattggt taaaggattt ctggtctgag gattcattaa tttatgaccc
    41761 agcttattcc aatctcaatt gcaatatatt tcatcgatca gatgacgata tataagaaag
    41821 aggattggta gctgaaatgc tgccaatcat aggagtgcaa attgaaaaca aaatctaaaa
    41881 aacttttgag gcttgttatc ttatttgtag cggcattctc catctctaca cctgcggtaa
    41941 tgtacttcag cacaggcgaa gtgacattaa atctttttgt tgagggtttt tggataattg
    42001 gccttctcca agcagcagta tttacgtttg tggtagactg ctcaagtagt gatttaggtt
    42061 cattaagtca atggcaaatt ggaaatgact gggacagaca aatggatcca ttttacgctc
    42121 atgaaattgg taacatttgg catggaacgc acttagactc gagttctgag gagtattgga
    42181 aataaaaaac aggagattta taaactcctg tttttttatt tccaaaagat ggttaaatta
    42241 tctatgtttc tagtacctta ccaattgaag tgatgatttg ttcgaaagct gatttgcctt
    42301 cttcggatac tagatcgtta cctccactat aactcattct aacttgataa ttcccttcat
    42361 caacctcttc aatctgaaca acaatttgtg caatactcat cttttaaaat cctctctgcc
    42421 taaagaaatt atgtacaaaa ccccaacttc gtttgtgttg ccggtattca ttaggagctg
    42481 ttgggaagct ggcccagata ccacctaatt tcatgactgc ctgctcgtaa tcgccattta
    42541 aaatagcttc cattgcacct tttttagcta aaattaaggc gacagcagct ttgtcttgtg
    42601 tggcgggact aaaatccgtg aagccatatt gttttgccag tcctttccat gtgtatccca
    42661 tgaattgata gcggccggag gcatcagatg tgcttctact accatcggtt cgagtaaaac
    42721 tacgtctaac agagttaccc gggtgacggg agaagtccgt aattctataa ttacctattg
    42781 ccgtctgata accatgtttt acaccttctg atgccgcaat aaaatctaaa aaggcgcgca
    42841 cttgcggatt atgaacatat cgctccaatt cggcttgatt atgagcagtt gaagctttta
    42901 atacatagtt tcctctcaca tgtcttccac ttgatattcc acgccacata accccgttgc
    42961 ctccatatga ggtggcatta cgcaacgggg ttattccttt gggcggaata cagcggatcc
    43021 gctagacttc gaattccctg gacgttgagg aattggtagg tcaaattcgg atgctaaagc
    43081 cgggttgccc aaatgggctg ctgcttgatc cgacccagaa cctatgtatc cttttccttc
    43141 acgaacgtct ttcattgcat tatcgtatct ttcaccgaaa gagttccagg gtaaggcgtg
    43201 agtattaatt ctcatggtta ccccattcgt ttgatcttga gcatttttag catgcgttgc
    43261 caatctacca acttcttggc ggtggctatt tctaccttga ttaatatcgc cttcaacctt
    43321 atcagattta tttgcgtatt cctgtttcag attctcaccc ctagcgttga tagcagcact
    43381 aattccatcc ggaattatac cggcctgatg agcttgggtt tgaacatctc caaatgccgc
    43441 cccagctccg gatgaaactt tatcagagaa gtgtttataa gcggcttgcg tattgttttg
    43501 aggtctatct tcaggggaaa ggtcattagg tttccagcct gaattttccg cagcagcttt
    43561 taaacgatct tgactattta cacctgcacg aattgcagca ttgtaggttc ctccctgtcc
    43621 tgcgtttaat tcactcatag tcaattcggg gttgtcttgt accaatgcat ttgtgttatc
    43681 ttgggcaatg gtaatgccat taccataagt ttgagctgcc atttctcgat aagattgagc
    43741 tttttggtag ctttctgatg ccatatgcga ggcttctctt gctttgctaa agttagcttc
    43801 ggcagtttgt gccaaactat taccagaatt gagtgcaact ttatatgctt ctgattggga
    43861 aactttgtct gtaaaattac tatcaaagct aataccttgg cgtttacctg cttcaatact
    43921 ttccccaatt tctgctacca tttggtccgt attttgtgat ttaccactcc aacctaaatc
    43981 accttttgct cctaaagcat ttttaatatt ttgggcttta gtactactgc ccgtattgcc
    44041 actaatgcct aacccagctg cgatggcaga tataactgct gatgtattag taactccaaa
    44101 tttttgggca atcttcttgc cttgatctat aacgtaagat aattttctac tttcagctgc
    44161 atccaaaccc attccgtaac cttcagaggc acttctatcc ataccataat tggttgcaaa
    44221 ggcaaggcgg tttgcattgc cttgagatac ggactctcga aaggaagctt gccattgttg
    44281 ggcagatgtt tctgctcggt ctgcagcctt cgaatatgac tctgattggc taatctgtga
    44341 ttgagaggaa acaccaaaac tactattcct catctgcgct ctactatctc cagttgccat
    44401 attccgccat gatgttccgg ctgcagattg agcagtcatc atttgaggat cggtatatac
    44461 agggtttaag ctatgtttgt tggcattgct attgttgtag ttcatattgc cggcagagta
    44521 attccccgac cctacttggg aacccgctgc ttcaccggtt gatttggccg atgaagtcat
    44581 gcccatcatg ccgttcatca ggctgcgtcc gcccatggca agagcacccg cgatgaccgg
    44641 aatcagccac attgcagccc cagcagctgc ttgtgcatcg gctgctgcca tcaataacgt
    44701 atcacctgtt cccggtgtaa taacgccgtt ggcggcttcc acgctaaaca gacgcttcgc
    44761 attcatagtc ataatcatat tgatgatact ggctacgggt gcccaaagct gaatccatag
    44821 catactcatc atgtagaagc ttaataaccg gaatgccatg atgccaccca acatggcgag
    44881 aatcattaca aaggggaata aaccaatcag aatggcctca gcagcagaac gaatcaaggg
    44941 aagattccaa cttgccattg atgagccggt tttttgggcc gcaatatact gattacgagc
    45001 ttgaacttga gcaaatgcct gctgtacggc agccggattt cggttgcgtt ggccattgat
    45061 aatgcctgct tgacgaataa cctcaaccat cgccgcctgt tcgatatttt tcaatgtatc
    45121 gaagcgggca ttatccagca gatattggaa tgagctattg cctaaggtaa ccagggcatt
    45181 tacccggttt attcctgccg aatttgtacc caaacctgca aaaacacgat cggcaatgta
    45241 gccgggcaag taatttttgc tataaaaccg ggtacggtct tttagttctt ttgccgcctc
    45301 attgcaggtc atggtctttg atttgccgtt atactgcaac atgttgcctc ttcttgaggc
    45361 gatctgcgat acattggtaa acaaggcttg gttggtatgg gtcaatgtag ccagaatatc
    45421 ggcactctgt gccagatcct gcacgttata gaagtgatac aggttcatat caaagaatga
    45481 acactgatac atgaacagtg cccagtcttg agtcagttgc gcgtccggga ttcgtgcttc
    45541 acggatattt tccatcaggc gcgttccgaa cagcatgccg gtgcgtgagt aattgaggtc
    45601 gtccggcagg tggttaacct gctcggcttt gcgggtaata ctttggccaa aagaactgac
    45661 aaagtagccc atataagcaa ggccgactgg tacattgccg acagtatatt gacggcctgt
    45721 atatccatac ccacctgatt cagtaatgag tacagtggtt ttagggacca tcatacccat
    45781 ccaacaaaat aacacgacaa acaaataact gaagttatcc ttcaaatcca atttgaagac
    45841 tgcaacagca agagtgacca ggaatccaaa taaggcagca gcctgcattg cgccacccat
    45901 catcgtagat cctgatattg tggcaacgcc attgaaaacc gcccgtacag cttctccgtt
    45961 accagggaaa gtaaagtatt cgacagccat catctctcct tagaaacggt tcatcgaatc
    46021 aaaagtcatg gagttgttgg tatccacaaa ggctgagcgc cattgtgttt caaactgctc
    46081 catagccctc atcatgcttt cttcgatctc gattttcttc attgagtcaa tgcgctcctg
    46141 gtaaattctg cttcgcaatt cgccaaggtt ggcaaacaat tttcgaaccg cttccccgct
    46201 ggcatcgtct gtcttgttat acatacccaa ggtcgagcgt acctggcgtt ccgattggtc
    46261 caaaatattg gccataatat caacggcaat cgcatctaaa accgctgttt ttttcaaata
    46321 gccagttcca aggaaagcat ctgatacagc cattttcagt atgggcaatt tactgttatt
    46381 gacaatggca gcaatctgct tgcgcgtaga taaattccaa ccttggttat tccttattgc
    46441 agtcggaagc gtttcaatgc gtttgtgaac catgtaccgc aaagaaggta tttctacatc
    46501 atttttctca gtgcagttct ccattaattt cttagaaccg tctttgcacg tccacatatc
    46561 caatacaacc ttctcccgga tttctccccc ggcattgttt tttccgttta aaagatcttg
    46621 gatgctgagg atttttggtt cgaaactttg aatacctact gtgtatttgt ccttatcccc
    46681 ttcatgttgt gttaatggag tgaacacaat tgttcccagc atgctgtaat agaagtccat
    46741 gtcgataccc agtttcgagc gttgacccgt atagttcttc atggcctcat aagtcaaatt
    46801 accgccggta aagctaatcg tatcttgggg ttttccggtg tttcgcacct tatcggcttc
    46861 atggacaagg tttttagcgg cctgacagta ccaagcacct tctgccggat catttgacga
    46921 acccgaatcc actgaagcgg cgcgacaatt tgaactggcc aacgaaccca gtttccccat
    46981 tgcgccgccg acaatatcct gactcagttt gcaactgttt aaactgtttt gattaacata
    47041 attgaccaaa tcacgaatac cgccaattgc atcttggatc atcggatcaa gggccttaag
    47101 tgccaggtca aaggctacac ctgctgcgtt attgcccagg tttcgcgtaa attggatgaa
    47161 ttgttcttta ttgacaaacg agaaagagcc ggcaaagaaa tctataccgc cgcaaccggc
    47221 actgattgaa ggtaattgaa tattcccaag gctgatatcc gacaccggaa agcgaacgct
    47281 cataccgcct cccgtatata ggctgccgct ttgaccgtgg aacgcacctg cttcagtata
    47341 attggcttcc gctcccattg agttaaacat gtcagccatg ttcttctcta tgccggcact
    47401 actattgatc ggaagggcca gcgatgccgc caagcataga tattttgcag aggaagcaca
    47461 aactgtcggc actttggccg gcttcggaaa tcgaaagatg tgaaatttca taaccgctcc
    47521 atgaagcggt tatagcaggg aaatccttac tataaccaag ataagttaca gtaaagactt
    47581 gagattaagg gaatcttatt ttacctgaaa tatccgcgtt gtcaaccggt taatacatag
    47641 aaatataaat attgaaggcc ccgtataggg cctttgattt gattaataca atgtcccgac
    47701 agggcgggaa tacgctaaga aacgatcctc aagcgtggtt tgcgccatca ccccaaatcc
    47761 aatgggtgta aattgcttgg tttttgtatc catgacgtac attgcaggta cagtagtaat
    47821 attcaacctt tgggcaatac cgttgttcgg caaagcacgc gggaagtgat taatacctcc
    47881 gccatcgaga ctgaccggca taaccttaat accgtattgg tcggcaaagt ttttcaaaat
    47941 cggcgccatg gtatgacagt aagggcaatc cgatttgaaa acaaacataa tgccgttctc
    48001 tccggcaagt tttctaattg ccccggcctt ttgttgctga cggttatgat catattgttg
    48061 ctgggccagt tggttgttcg gacgaccgcg ctggctgtaa tccaaatttg ggttttgcca
    48121 aattaccctt tgccattgat cggcaaatgc cgcagatttg ttcatcacaa cctcttgaag
    48181 ttcaatatat cgcttcaaat tttgaggtgt ggggttcatg acggcaattt tacgcgactc
    48241 atccaaattt ctttgcagca tttcaaattc tgcaagttca ttacgaaccg gagtattttg
    48301 aggccggttt tgtccggctt gtttgggctt tttagcatcc ggataccata agaaagcacg
    48361 gttttcgttt ttccagatat cttggtcata ccatcccatg ccatcatccg cagctcgcag
    48421 attggaggcg tgcaggctaa atatcagcat gacggccatc cgcactcttt tcatttttta
    48481 ccaccgttct ttctgttcgg gcacgccccg atatccataa tttcagacgg aaaggtttgc
    48541 gacagtgcgg gcgaagtatg gaatgtcaag cgaaccctgt tgcatccggg tttgttttta
    48601 atttcttcca ttctgaccgc ctcgacaaaa ataggtatgt cctgccggaa tttctgtttg
    48661 acggcagccg cagcacggcc gctgaaatac ccttttgccg aaccgtgacg cattgccagc
    48721 gcaagcaagg catccggctt gccgtcagga agcgggatgg aagtccttgc tagagcttct
    48781 gttttttcca aagcgatacc gccggtataa ggaagggatg cggcaagtgc catacctgta
    48841 aaaaacatac ccgatacaat acccgccgac gcagccaacc aattattgat attcataata
    48901 gttcttcact tccaaatttt tcccattaac ggatgcctcc ctgatttttt gtgaaacctt
    48961 ttgcccctgc ttggccacgt tgtccatgtt ggcatagatt tcttcataaa acccggacag
    49021 atcaagtttg gaaaagtcta actgttccaa ttcctgtgcg gtaaatcctt cgcatttcgg
    49081 gttctccggc gtaccccaac cccgtttcaa ttgctctttg ccgccgacat tgactatttt
    49141 ggctaatttg gaaatgaaac agcactggct ctctatcatt gttacacatg cccccaatat
    49201 tttgctgccg caataagaac cgacataatg gcacaaatct gctttacgct tcatggcaac
    49261 taaaatttca ctttgcgggc acttcaacca ctgttggatg gcgattacgg caatagcagc
    49321 cgcaaatacg gccgggttga agccgacaat tacccccata ccctgttgtc ccgcagcaga
    49381 gaaacccaac gcaccaatct gagatgttcc ggtaataccg ccggccgcac tcagggccgt
    49441 acccattgcc tgtgctgcgg agctgacaaa catagtggta aacgtataat ggctggcaac
    49501 gctggccata aaatacgttg ttgccatagt tgtccctgtc tgtaccgcga agtcaacaaa
    49561 tttgcccgga tctgcttttg ttcgacagca gtctccgccg aatacgctgt ctaaagcacc
    49621 atccagcttg atagagcatt gcccggcttc tccattgaaa aaacgctgtt tttcgggatc
    49681 ataaaaccgt tgcgtctcac gggcgaattc catcgtcgtc atagttttgg caaaatcccg
    49741 atcctgacaa ccggatacct ctttggttgt ctctcctttt tcggtacatt caaactgttt
    49801 ttctctggct tgacaggtac cgtccggcag ctgatgcgta cacttgtccc caatcagacg
    49861 gcattggtct gaggatgtat aagcgctgca tgtttcggaa tcgcttccgt ccgtacattg
    49921 cacggtgcgc ctttctttcc agcaatcacg tgtaacgggt acgccgttga tggtcttggt
    49981 actgccgggt tccaaacact ctgttgcaac tgctttacat ttttttcctt gtacctgaca
    50041 tgacgactca tcccattgcg aactaatcag atgggtatgc tccatttctt cgactttggt
    50101 cggaagcctt ccttgatgta acgttctcaa atccacatcg caagaatact tgtgcgtgta
    50161 acgcaaacac tccccctcat tcgactgctc caaacaggtt tgtccctgta cttcacattt
    50221 tgccgaaggg tcttttaacg gtgcgcaata atcggcagaa tccttctcaa ggcactgata
    50281 ctcttctttg tattcccaac agtctttagt tacgtcgacc ccgttgatgt ttttggtcga
    50341 cgggccatca acacaaacgg aagacgtccg ggtacacgct gcactttccc tgagtgcggc
    50401 agcagacgct gaagtgatta acataaagtg gcagatcata accagcaaag acgttatagc
    50461 cctcattgtt ttaccctctt ctcatactcc gaacaactgt tttcccattt atcgtgacaa
    50521 acgggctcgc agtattggtg tacattaaag aagatagcat tttctccgcc gcctccgaca
    50581 acggtgcggg tccaaatggt atttttgccc tgatgcagat acggccgtat gtcgatgttc
    50641 aggtttttcc tccaggattt acctctttca gcgttaccca accgagtacc ttcctcatta
    50701 cgtaccggtg caaaccttct tcctatagga aatccatctt catcttttgc agtataatct
    50761 gccgcaaaca tctgattgtt atagctgctg tacacaattt tgccgttgac tttgacaatc
    50821 agccaatcat cgtactcaac ccggctcaaa gtaaacagcg tcagtttttc cggatttttc
    50881 acattgagcg tcatctcccg atcatactgc ccgtcacgcc aatagttgtc gccaatagta
    50941 ccgaacatca gcctgtgtgc gcttcccgaa gccgtcagcc tgaccgtcat atcaccctgc
    51001 aggctgcctg cttctatacc cgaacgggta cagtttggtc ctgatttctc gcataccacc
    51061 tttaatttct tgccgcattg atactgctgg tttcgatatt tgctctccag gcaggaatag
    51121 ttccagccgg gatcgacatt gaccttaagg gccgcctcgc acaagaacat ctctgcgcca
    51181 tggtattcat tgcaacgttt gacggttttt accgcaggaa cggtaatggt ttccgtagga
    51241 catgccgacg agggaactgt gccgacgctg ccgtcttcat tgacgggata tttccatttg
    51301 tatttttcca gtgtttctgc cggatcgcct gcaattttct gcgtgccttt aaccaacttt
    51361 tcatttgaag atatggttac atcgggccgc ttggtacggc ttcctgcaat caggtttaca
    51421 ccttcgcatt cttgacgaag atagagatcc ggattggcag gcagattttt acagtcgttg
    51481 aatttggcgt caccaaactg agtcggaaga gaaactccgc caccgtaata ctgttcagcc
    51541 attggtacgt tgtctgtata tcttggggta tattgggcag gctgcatcat gtttgttgca
    51601 gtcccggccc ccaattttcc cagttcgccg acgtaactac cgtaatctgc agcaaaggcc
    51661 gtctgcatga ggccaaatac agttagataa atggcatgtc ttttcatttt ctgccttcca
    51721 gaacggctaa tttcgcattt gcaatatcgg aaagtttttt atcttcagac tcacggatca
    51781 agtgttccaa agccgaatgc aaggacacat caccctttac ggcaacatgt tccgtacaca
    51841 tccccaattc tgaatttaca gcgcaaccgt ctaaaccatc ttcgctgttt cgggcgatta
    51901 ccgtaacagg gacttcggtg atgccgtaat ggtcgaagag ttcgggatga atcagcatat
    51961 cgccttgcaa agccgcaagt tcttcggccg ccttaaccgt ggctttcagt gagtcgtctt
    52021 taaacccgcg gataaccatt accccgcctg cctttgctgt ttcacgggca atgcgtttca
    52081 agctggcttc gggcattgaa aatgatacga ataccattaa atccgctttt tccgcagcct
    52141 gtcgggcttc ttgtgaggca gtgcggtatt tttgtgcgat agacatcgga tcagccttga
    52201 tttttgcatt tccgtaatca cgaacatcgg gcacacggtg cttgatacct ttcaaatccg
    52261 gaatttcagg ttgctgccga ttgtcaccca catttgggtt tttaactcgg gtaactttac
    52321 ccatggttgt acgcagacgg tttcgcgccg cctctacatc cgcatcggcg taagcactgc
    52381 ctactccggc caaaccgccc aaaagaatga ggaagacgcc tccggctacg attacattgc
    52441 gtaaagactt gtccatcact ccatctcctg tcaattagtc ttaaatttat atgcaccgca
    52501 gcagtcgcgc ttacggaaaa gctggaaagc aaactgatta cctttgtatg gaaatgcttt
    52561 gccgctgtct ctgactaatg ttgaacgtcc aataggctca cagcaacggc ctctgaattt
    52621 tcgggcggcc accggatagg tcatagccat tttatagttc tgtttatcca ttacgggatt
    52681 aagtttgtac ccgcataaag catccctgcc tgatgtcctc caagctacta agtccctgtg
    52741 catttttgtc tgaattctag ccgccatcaa tttagcggcc tgcacttcgg agaagtggcc
    52801 ggacatccat cctgacaaag ggtaaatcgt tccgtggcaa cctgcacacc aatacaattt
    52861 attgaatgga aagccggcgg ccgctttaac acagtcagcc gcacaggctg cttgtgcaac
    52921 gacattggca aagagcgcag aatcaggggc ttgataaacg gccaatgacg gatatttcca
    52981 agtgggatca agttccgtca tccaaccgat atccaaagta cctttttcca tacaatcgtt
    53041 gtccaataag gcttccaacc aaaatacgac cggattttta taccaatgaa cttgataaaa
    53101 ggcattatgc tgatcatgtt gttcgttacg accacgctta tgcgagccgc caaaaagatt
    53161 ttgaatctta ttggatacgt ctgctttacc tgttgtttcc gccgtatctg ctttttgatt
    53221 tttaagtgaa ataccgccta ggctaaccat acagaagggg gtgcgtacga cttctaccaa
    53281 gtgtgtgggt tcccaaaatg ataccgtcac gcctgaacgg ctcgggtgcg tacacgaaca
    53341 aaaccaattg tttggagaag agccgttatc ctcttgatta tccatattca gattgatttt
    53401 gccgccgatt ttgattggga acatacagct ccaacaaata tcatttatcg gattaagcat
    53461 cttcccctgg caggttaatt tattcggtat cggagcagta ggaacagctt cagcggcgtg
    53521 agatatagaa aataatggga gtagtaataa cggaatcagt aatttcagcg gtgtgacaaa
    53581 tattttcacg gtttcatgac ctctacacgg agccggcccc cttcttggga gaccaaactt
    53641 ggaacttcat tcagctgaaa acttttggac atccgccctt cttggtcgta ataaaccgcc
    53701 tgatccattt ctactgataa ctcataccaa ttgcctgcag tcagaatagg catgaatgat
    53761 tcgccatatt ctgctgcaag tttcttggcc agttcgattt ggtcaggttc ccggccgtca
    53821 aagaaaaaca ttttgaatgg gattggggct gcttccaaag gtttgacaac agtaccggca
    53881 ggagcaataa tttgaccgtc ttggtcataa attgtttcgg ttaaggtata acttgggtcg
    53941 aagtatcgaa cagagccttt agttgccttc gccagtcctt ccaagggctg tagatttaag
    54001 gcggagcgat ttattctatc ttggacctct ttctgcattg ccgccaattg gccgctgtct
    54061 tgtttagctt tcaaacgcgc ttcaatttcg cggattgcat gtttttccgc tattgggtat
    54121 gtggtgccga ttttgtcgcc aaccttctcg acagagggtg tcgaagcggc tacccatgag
    54181 gaaattccgg ttgcaagtat tactagcgat gcgactgaag ctattttttt cttgtgggac
    54241 atactcactc ctaaaaaatg gggaccgcct taccaataat ttgccctgaa tggattaagc
    54301 cggataattc atatcgactg tccaagctgt ctttgtgccc tgttttaacc cacatgaacc
    54361 gttgtggaat ttccccttcg aatgggttga tggccagctt catgccatcc agggagtatt
    54421 cttttgcagt acctacctct ttcccatcta tgaaaaaagt acgattcgcc ttcgttacat
    54481 gatttccggg aagtccggca acctctttga cgacctgcgt accaataggg taaaaaccac
    54541 cttgccatgc aaaggccaca taatcgccct gcttcagatc acttagcttg ttcgcatctt
    54601 ttttgatgaa gtaaagatgg tgaggcaagg aacgggaaag attaaatgcc aaagcataat
    54661 gttgattgaa tgccgacaag cagaccgcaa ctgctaaaaa aggggttgca tatttctgac
    54721 aaaactccct ataaagacgt ttcatcactg ctttgttgct ttctttttgt gatattttta
    54781 tcattgcctc aaacattaac gatccatttc ttcttcagta accggggtgc ctttaccctc
    54841 ttcggccatc ggtttcggaa ttgtcttttc aacttgttga tgtaccggtg ccgccactac
    54901 agctgtggcc gggacacgct tatccaactc tttcataatg tcagcagtaa catcaaccac
    54961 ctctgtgttc cgtccgacca atgcagattt gacaaagatg ttacagccgc accgaattac
    55021 cttttcttcg ataacctgtt gcaaggtatt catctttgct tggtagtttt ggaggatcgc
    55081 ggctcgttgc cggtcggaaa ttccattctg cagtccttga ttcttcgcct gttcccggta
    55141 agtcatggag ataccggcca aatctacaat atggaccggt ttctcatggg ttttcatgaa
    55201 attccatacg cctaatgcca atgcggcaat agaaattgcg ccgatcacaa ggctgcccaa
    55261 tgaaacctct acgccgttat cctccggatt cttttccgga tcggtatttg ccgtgttttc
    55321 tttcttttct tttgcttcag tcatcttatt aatatcctat cctctgccta attagcgggt
    55381 tgcggcacgg tcccttaaaa ctgcttcaat ggcctcggct gtattcaacc cctgatttct
    55441 gtaatggttc acggcttcaa cgtcttccgc ttttgagctt gaggcaagaa cgttgaatgg
    55501 gtccgagtac aacctaccta ctccataacc tatcggggta gagacaaata tttcactgta
    55561 gtagtcagtt ttgctcaaag ttcgcagaat ctgcataatc ccgtcattga cttcaaaagg
    55621 ttctttgtct tgaatggttt tgagcgattt agccccggta cgcaattgga aaacaaagtc
    55681 ggcattctct aatgccgcct cagccgcctt agtcttgtag tagtcatttg caccttgcgt
    55741 cagagtgccg catgcacctt tgtacttacg gacacggcgg tagccttcct caataaaacg
    55801 agcggctgta cccgtgtcgc ccaacaaagc ccaagcctcg tcaatcatca taattttgta
    55861 ctggcttcgt gataggtaca tgccctgcat aatgcggtac ataatcagct gcatgatgat
    55921 ctgttgcaga tccggtttgg tttttaactc ttcaagttcc agtacgacca ggtcattttt
    55981 aaactcgaca ttcaattttc cattgaagta actgccgtag ataccttccg aacagtatgt
    56041 gtacaattgg tcggcaacgg ccaccataga caaatctcgt tcaccgggct ttcctccttc
    56101 attactgccg gtaatcaaac acttgagaca gcgttccctt acatgatcga ccgtgccttc
    56161 ttgtccgaat tcgtaccata cttcttcgat tatccgttga agcttggctt cctgctgttc
    56221 cgttagggag tctgacggag aggccatcaa tgagaagatt agtttcaaca tctcaatatc
    56281 ttcgttgatg tccgttacca tttggaatgg cgggaagcac aggcctgctt cataggtaaa
    56341 ttcaatatac tgcccgccaa tcatttggca gaatttttca tacgaacggc caacgtcgat
    56401 aatccatacc tgagtcccca gccccatgta gttgcgcaca atttcattgg caaaaaatga
    56461 tttgccggaa ccggaagcgc caataatggc aaagttatag ttacctgccg ggttggcaaa
    56521 taagtcgatt cccatggcct gcccgcgttt tccgaacaaa ccgatgacag gtcttcctaa
    56581 accgtgccac tcgccgataa cgggagacat acagacagca ttagtcatgg ttttggttga
    56641 ccatcttttc ttactgtcca aatatttctc aacctctttg tcgatagcca ttgggagggc
    56701 agtcaaatat gaccccagtt gaagatattg gtcacgataa agaccgaagc cctgagcctg
    56761 ccaaatcgat accgcctggc tttcagccaa gttaatgttt tcgggacgct cccaaatgac
    56821 aacttggtgc aacaaactca ccagaccgcc gccgcctttg ccgaacgctt ctaggcatat
    56881 gtcgtaatca cgtttgattt ttgctgcctc cggcataact gcggccatct tggattccgc
    56941 cgtttgtttg gcgcgggccg ctttcagttt tacggtattg ctggaagtgt cataatcacg
    57001 cttgaacatc gccatggaaa tcagaaatgg tgatgtgtaa ttcatgtttg cctcaatgac
    57061 atcaccaatc aatgcgccca tattggacag atgaaactct tcagggtagc gattggcaga
    57121 atagcattga gcaatcacgc aatcccccgc ggcacggttg ccaaaacgca gccccgagcc
    57181 gctatcaaga accttgatag atgtgcgggt ctcaatcatt tgttcactca agaacttggt
    57241 ttcatcccaa acaggatcga tttcatcttg ttgtccggta aacatcctat tagggttgag
    57301 taggagcgtt gtccaagcca ataaatcgtc tgcattccaa gttcggccgt aaatctgcat
    57361 ggagcggagt gaatcgcgca tgcgggcaag gagcgaaacc gccttgctga cttccactgc
    57421 attatcaggt ggaatattca aatttacgct gaggatacag cgcagatttc gtaaacgaat
    57481 ggccgtattt tctaccaaga ccttctgtgt acccttattc caaaattcag tacgtttttt
    57541 ggccaaagtt tcgaaagtgc ttcgcatcgg atcgtctata ggtcgtgatg cttgtacttg
    57601 ctcatattcc ttgaagtaat ttcttaaatc gggggagccg aaaatttgaa cctgtatcgg
    57661 cgtcttttca ggaagcagcg catacaggct gccccactta ccgatcaggt cgtcactgcc
    57721 gcctaaaaat gggttaagtt ccagaataaa gcccatgccc aacggtttgt cgcctttaac
    57781 ggacatcagc gtaaacaact ctgttttggt atcgtatgca ccgtaattga gatagtcgct
    57841 caaatggtca aagtcaggat cgacagggtt gttgtgcaag gcttccatcg gttgatcgac
    57901 atcatccgaa ccggccaacc aatttttaaa ttttgacagg acgcccatta catacctcta
    57961 tcgaacggta ccgggaatac ccgatgtttc ctgcagatga agattcacgc caacatcggg
    58021 gatgcggtca tttgtgttgt ttgaattggt tggagaagtc tcaaccgaac tgcctgaagt
    58081 tttggcccca tcccccaata agcggatggg acgatattca tttataatct gttgcccgtt
    58141 gtgggcaatt aaccaatcac cctgattcag aacgacatat gtgaaacttt ggtcatttaa
    58201 atcgccgtcc gtatcaagat agggagcgac ccaaatgcga gcaatttcac tttgcgagcg
    58261 aacgggcgta ccggaggaat aagcttgcgt taacattaat gtgcgaacac tttgatcagt
    58321 gtaaggttgt ccaacctgtc ttttcatacc gtcttttaaa acacctgcaa cagatgcttt
    58381 atagttactg cttatagatt cacaataagg atcgtcactg ttccgggcat tagggcatcg
    58441 gaatttttca ctaccgccga taccggacat ggttaaggtt gaacatccgc taagaatcaa
    58501 tattgccgca gtaatcgata aggctttcaa acgcatcatt ctgctcccgc tccatttaac
    58561 catttttcca attcggcacg cggcatggca ccggatgtac gccgaccatc tttatgaatc
    58621 atgcttggag taccgcgaac tttaagctgc tctgcgagtg caatattttc tgatacaggg
    58681 tttttacagt taccggccgc cggttcttta tccatcagca tgtagttatt ccatgctttt
    58741 tcacgatctt tactgcacca gatatgttct gcttttgaaa ttgcatcagg gtggagggat
    58801 ttaatcggga acatgaaaac gtatgcggta tagtctgtca tgccggccaa tgtttcttcc
    58861 aaacgacgac agaacgggca atccgggtcg gaaaaaagtg caaattctcg cttgcctttg
    58921 ccgccattac ctttaacgat tttgatggcg tttttgaatg gcagtcggga ataactcttt
    58981 tgtgttaccg ctgaacgggc catctcggtc aagtctttct gctcttgcat atcgtacaac
    59041 gcgccaaaaa tgaaatagcg accgttttct tgtacatagg caatgttgtc acccattgtc
    59101 acttcataaa ttgaagccat gggggtagcc tttactgaag aaaacgtggt attcggatac
    59161 aacttgtgca gagtgcttcc aacagttttg acatcggcaa aggccgtctg aaaagctgtg
    59221 attgctgcaa tagtggccaa gattttcttt ttcatagatg gatcctattt ggtttcgccg
    59281 gtatcaattt cgataccttg agtcagtacg acatcgactt gacggcctgc attgacttca
    59341 actaccggga agaccttatc ggccagtttt atgtaatact cagcaagcct gtcagctgca
    59401 ccaccgatac cagatgcaat ccctgcacga tattgatcac tgccccgaat agaggttgtg
    59461 ctgccaaatg cggtattatt cgtcaccttg aatgcttccg atacgccctt acccataccg
    59521 ctaatcacgc cggacatcag tgcatttgcc aagagttggc cttgtttcgt tacaagatta
    59581 cctctcacgc ctgttttgcc gtcttcaccg gcaacgtaac cttttaccgg catgtcgatg
    59641 gctcggccat cggtacctac acagctgaga ttctgcagtc tgatgtaagc gcgttctgaa
    59701 gatatatcgc cgtaagcctg cccggttacg aaacattctc tgaaattgta tttaaaccgg
    59761 ttgggaagtt gcgcaaaatc cgttacgcgc attaaaacag ggtgggggct ggcattttgt
    59821 gcctcacccc cggtcggtgc gtcaacccca cctaacagta cggatcggaa aaaagtaccg
    59881 ctaggtatat acgagtcacg cgcccgaaac ttgccttgcg agacgacaac cttaccgccg
    59941 tcttcaacta cttctatatt catacgcggg gtttttgatt taacccactc cgcttctttt
    60001 aattgcgggg aaacccgtac attttcattc ggatttccgg tatttaccga tccggtcgtt
    60061 ccaggttgca gctctgcttc actcatactt cctgaaacga ttttccgttc aatgggccta
    60121 tctgccggag gcgccccgtt cggcaatgtg cctgccggag gggccggagg aagccggccg
    60181 ttatccccga taccgctaat tgcaggcggt ttgcctaatc catcaggccc cagcccctta
    60241 cttgtatcag gattactgct gttctctttt tcttttaact cctgaaccat ggtttccaat
    60301 tcgctgattt ttcgatttgc atattcaatc ttcgaacttt ctgtgttcat ccataaggaa
    60361 ttgtccgtaa tgcctgcttg ggggcttgta aaatcagttc tgagaatctg ccgtttgttt
    60421 tgaacctgtg tgacttcggc agatttcttt ttattgcctt caatgacata aattgcactg
    60481 cccacaatcg ccgccatgct gcccgcaacc atgcccgcga tggctaactg gcgttttcgc
    60541 gctgctgaaa gtttaaattt gtttactttc accctcatct caacctcccg gacggctgat
    60601 aataaatacg tcggtaattt ccccgggttg taggatctgt ttccgaaccg ctaccgccaa
    60661 aactccttgt cggtaaaatt cctgctctct aatttcaagt tgtttagtac ctaaattagt
    60721 taatgtaaaa gaaaggcctt gcatatcagc ggcggtgtaa cttccgtttt gaataaagag
    60781 ggtgtttttc cataatggga cggtttgata ttcatgacta tgattgatgc ccatatcgcc
    60841 ggtagtgcca cgcatcatag aggccatcat ttttttgatg gcccgtacat attctgtact
    60901 attcatcgtt acaggcattg gaccggtttg cgaagccaat actgctgctt cacggcgatt
    60961 agctccagcc acatcgatta cgatgttgtc accctgtttt gatgtaggtt taagaaccag
    61021 caaataggtc ttaccttctt cgcttataac gaaaagattt gtcggattgg aggtcaaact
    61081 acggacaaac agttggcctg ttgttgtatc tttctgaagc tcaagatcac cctccataaa
    61141 tttccatgat gaaattcgac cgccttcaat ggcgattctg ctcaagttcc gtttacttat
    61201 tgaaacaacg tgaggtgtat tttctgttgc cggcactcgt tgcgctgcaa aactagtcgc
    61261 gggtccggct gccaacaagg cagaaaagac aatttttttt aacagtgaac acatatcaat
    61321 tacctcgatt accgccttta aattctgttg ccgttttact gatttctaca tcctcggttt
    61381 caacttcgga tttttcctga cctgcgggtt ctgctttagt tacacgcttt tgaacttctt
    61441 taatcgataa taattgcagt gttccataat tatttttgaa agaaataagt agctcgcggt
    61501 ctttttgctc taccagggca ttgttgcgtt taacctcaaa aaccccacga atggaaactg
    61561 tattgtctga aatgtccgcc gttgcttctg tcgcgctaaa agtggtgccg acattatttt
    61621 tcttaaccca taaggcattt aaagacagct ccctctctag ggcatcttta taatcaggtg
    61681 aaacaatctt caataattga ctattctgat aatcgactgt tttaggggtt acatttaaga
    61741 gaagataggc agcataggga gccatttctt caagatattc tttagaaaaa gcgatgtttg
    61801 atacagtcat tgaccgccgg atttccggcg gggtcagcgt cgtttgaaca atcgtacctt
    61861 tgaagagcag ggtcgcggac aaaaatagat tgacagctat agacattccc aaaagtatcc
    61921 aaaaaaagac ctttatgccg gttcttatcg ccaattcact caacatatct tcagtcttca
    61981 atttagaaac tcccgtttat tcgtttcagg tatccgtttg tttttttcat taatgggtaa
    62041 gtaccagtaa agaacatgca aaaaaaacca ttgatgcttg ccagacttaa gcttgcccca
    62101 cctccatgca atgaaaaggc taattgacat ggaaatgaaa aagtgacgag agacaatacc
    62161 tataccgaag ccggacataa ataatataaa aacgtccaag tcccaaataa atgccttttt
    62221 gggatcgtct atcgataccg gcacatgata atctctcatc acttctctta tctagtttga
    62281 ttaaataatc aaggtaaaca tacctttaat gatgcccggg cctaatgttg cggccaagcc
    62341 aacacccaaa ccgacgatac atggcatgat ggtttgtttc atcaggccta ccgccaaacc
    62401 gatcaagaat gcagtcaagc ccaaagccat acccagataa ccttcagacc agcctgttac
    62461 catgtcagcc aagcctttga attcggcacc tgttgtagcg gccattgcca agttagcata
    62521 caatgccaat gcaaccgcac tagcggactt tactaattta ttgcgtttga tattcattat
    62581 gaacctcatt aaacagagct tatatcgcca agcttttgcg tccgatcatt ctttaccaag
    62641 tgattacaac ggttctcgta tcatcactat tgcagcaggc cggggaactt gctgttctta
    62701 tttttgcgga tacattatgt ttcctcagcc atttacctat tactgctgcc cgttgtttag
    62761 ccaaacgtct attaattgca gtatttcgac ctccgatact gtcggcccat ccgcgtaaat
    62821 aaaccgtctg agtatttttc aatttaggca gaacacgttt gagcgttctc acgccctccg
    62881 caccgaggtg cgaactgcct aaattgaaaa acaccctgat ctcatctttt tgaagattgg
    62941 tttgtgtttg agcataaacc ggttgttccg ccaaaacctt gcgctcaaat cccgaacact
    63001 cttcattgcg gcagaagaca aattcgccca gctgattttg ttccactgta taagttgcgt
    63061 cttgaggcag cccggccact tcctgttttg gggcggcaca ggctgtcaga atcattgcgg
    63121 aggctacgaa gagcgtgttt ttaatcgttt ccaattttga tataccttat ttgcgtaagt
    63181 cattcttgcg cgatagcacg cccggccttt aagtttggta catgcggcgt tataagcacc
    63241 aactgcttcc catgatttcc catgttgccg ataactattt gccaatatcc atgcaccaat
    63301 atgtacattt gtgcatggat tccatacatc cgcccctgtt aaaccaaatt tcttgtggag
    63361 tacgggtagc catgaacggt tgatacccat caagccaata tcagctgtat gactttcaat
    63421 attcggcctg aaagcgctct cggttaatgc gatcgattgc aacatggcgg gatcgatgcc
    63481 atatttgctg ccggcttcct tataacaaaa agcaactgct cttgcagatg tgagtaccaa
    63541 aacacccggt acgacgaact gtaaaagtat ggcccttaat ctcatgtctt tactgtcgtt
    63601 gtatgtatga ctaattttta tctcgattcg acaatttttg ttaaatcaaa cgaaattaac
    63661 taaatttttt agaaatgttt ctttaaagat agattatggt ataattaaaa gttacgctta
    63721 tattttatga aattaccgat tcataacata ttgatataaa aaggaatacg ataagggtaa
    63781 aggctcttat ccaaaagcca aaaggccgtc tgctaaaaaa aatcgcagcc ggcctttttt
    63841 aaaagctgta acggtaaagc aacagcctta atggttaaag gaagggttca agggaaaaag
    63901 ggtaaaagtt aaaggtaaaa caaaggtctg catgtaaaaa tgcaggcctt tgcctattaa
    63961 aaggggcagc attatacctt ataaatctta ttctgtgaca attctaatta aaataacatt
    64021 ttttattttg atttttgaca tggcaacttt tttttattat atgaatggtt tgacaaccct
    64081 gtctttttaa tcaaaatgaa tgaaacagat cttcccaagc ttcttagtgt cataaatcgg
    64141 tttacggata tcgataaaaa ctggtcttgc gtgttgttgt tctggataca gcgtgctagc
    64201 ggttatctgg aaatgatgac gctggatgac gatgaaaata cggcaacctt ccttatcgaa
    64261 aaactagaga aactactaga gccactgccg atagatattg ataacacgac ttttgcagaa
    64321 gcacttgccg atgactttga aatcttattt ctaatggccc aatacggttc tgaatactgg
    64381 aattcacttg aagaaagctt tgaggaattt tgtattagac atactacgct acctaatcaa
    64441 ttgattgctg atattccaca tgcaaaaaag gaaaaagtca ccatgtggct aaaatctttg
    64501 cttaaatcat catgaaaaca agccttctca ctattgcatc ctcactaatg cttgtcagcg
    64561 gctcaatgct atacctgata aatacgagaa aaagtacaga ttccgtgtca cataatgatg
    64621 aggttgccca tatcaatcag aacatagtaa atgaccggtt tcgcatttta aacgcgcatg
    64681 aactgattca ggtgctcgac ttaagccctc aaatatccgg tattaagatg aacttagggt
    64741 tatcggatga aaactggagt aaggatgccc ttcccttttt ggaaaaatat atagcctttg
    64801 ttcaaaggct tcccgccagt gagtctcatc accatgcagg tgatggtggt ttagtccgcc
    64861 atacactcga tgtggctgca ctggctcttg tggcatctac atcgcaatca tggcccccaa
    64921 atgccaaaac cgaggaaata gcgaagaaaa ctgccgtttg gcgttatgga atcatgtgtg
    64981 ccgccctgct tcacgacgtt ggtaaaactg tgacgggatt tcaggttgaa ctgtttgatt
    65041 cggctatcag tctcgaaaaa ttattatggt tgcctgatac aggctccatg gctgaaagtg
    65101 gcaagcttta ttatcgtgtc gaatttcctg atgcaaagtc tgcttacagt acacatgccg
    65161 aaatcgcctg gacattcttt caagctttgg ttccttcaca tgtcagacaa tggcttgcca
    65221 caacggatcc gaatttaatg attactttga gaaattatct gagtgggaaa aaggatggca
    65281 gtcctcttga gcagttaata aaaaatgcgg atatgacctc agtttcaagg gatttacgaa
    65341 gcggcagccg tcaacgcttc tcaaccgcca agcgtaaacc attcattgaa accattatgg
    65401 aaacattgaa ggagatgtta tccgatagag gagtccattt cagcatcgca acaactgccg
    65461 gtggtgacct gttccgtaag ggtgatcgca tctatttgat gtcaaaaaac gtacctgact
    65521 acatccggca gtatttgagg aaaaatcaac atcctgctgc aggctcattt ccggctgaca
    65581 accaaaggat atttgacaca ttattcgagt accgtgccgt cattccgccg gaaaacgatc
    65641 ctcacagggc aattaaccat atagaggttg agtttacccg tatggacggt gaaaaggtcc
    65701 gtaatatctt cagtgtactt tgtttcaatg ccaaaaccct gtatccggac ggagattatc
    65761 cgactgagtt cttaggtaat ctttcagaag tttccgccaa gaaggaaata ccggatgttg
    65821 tagtatcaga ggataaagtg tcaactcaga tacaaggtgc cgaagataac ccagtcacta
    65881 tgaccaagga caaaatggac ttcctgtcta tccaaccggt gacagcagaa caacctcccc
    65941 tacctgaaaa aaatcaaact gagacagcta actcggcttt tgacaacagt atgaatactg
    66001 ctgctccagc ggaaaataaa tctagcgagt cagagaaagc taaagaaccg ggttacggca
    66061 caattgataa tttaattgaa aactttaacc tgatagaagc tgattcagaa caaacagaat
    66121 caaccaaatc agaagccgag acagcagaaa atgccgttaa cctggttcaa gaaaatataa
    66181 gagtagaagt taaaactgag acagtggaca aacctcgcgc tgaggttaaa gaaactgaga
    66241 cagataaagc accaaaaaca attaaatcag gaaccaaagc aagccgtaag aaacttgccg
    66301 gccttttcgc agacaatcgt aaacctattg gaaaaacagc cgaacccgat gcacagatta
    66361 aaacaactga gacagtaact catcaaatag attctgaagc agtttcattg cctgaatctg
    66421 aatcagcaca aaacggtatg cgtgaactgg agcagctaag actaagagaa cctacatcta
    66481 gtccacgccc ggttcaagtc atggatgact ccgaaggttt agatagcctt gcaagcgggg
    66541 taaaaaatat ggccgaactc gaagccatag tttctgaacg taatttgaaa aagcaatcag
    66601 aaacagagtc gaccgaaata gtcgaggatt cagtcaaagc caaacatatg gaggccagag
    66661 accgtggaat gcagttttta cgatggctgg cagatggatt aggagacggc agcatagcag
    66721 tcaatagaag tggtgcaaca gtccacttta ttgaacaggg gatgcttttg gttacgccag
    66781 ccgtatttcg agattacgcc ggcggcgtgt tcaataaatc tgatacggaa agtctaggcc
    66841 ctcttacaca aaaaggcttt gaggctttga ggttgcatgc aagaacgaaa aaaagctcat
    66901 tgcatagggt tctgactaca aacggtagca atcgcagact cttctattgc taccttatcc
    66961 ctgaacataa tatcaaacat atcatacagc caggttcaag acctcagaat aataccgata
    67021 ttaaattaga tgaatccgac ttattagtaa tggaacaaaa atgagtgccc acttccctga
    67081 aaacgtttat cgatcaccct atgaatggat cagtgtagcc gcatactcca ccacagcggt
    67141 ttcagtagca catttgcccg tacctccgac aattgcttat ggcagtttag ctattttttc
    67201 gttggtttca gtcaaacgtg cgttatcagc attacgcatt atgcgtttcc gtaaaaatat
    67261 tcggaagttg ccctattatg cccttactgc cgatgaaatt ccttcttcta ataaagctct
    67321 ttttcttggg aaaggtttta gatggacaca gacacatacc caacgtcttt ttatggttcg
    67381 ccagcctgag aacgacggac ttcgtaaccc ctcaaaactc tataaggcag cgcgaaaaca
    67441 tgagattgag acaatgggta cagactggat ttcccgcata actcggaaat cggcatggtg
    67501 gaatccggtt gctcccttac ctccggtcgg aggttctcct gaattacatg gcattgaaat
    67561 tgaggaagat gatatttggg aagatttggg ggagcgtgtt ggccacaagg ttgtattggg
    67621 cacaacccgc gttggtaaaa cccgattatg cgaaaccatg attgttcagg atattcggcg
    67681 gggggatgtt acgatcgcat ttgatccaaa gggtgacgct gacctgatga ctagaatgta
    67741 tgtcgaatcg caaaaatcaa acaggccgtt ttatatgttc catttgggtt tccccgagta
    67801 cagttgccgg tacaacccta tcggggatta tggccgtatt accgaggtgg cgacaaggat
    67861 tgcaaaccaa ctgccttctg aaggtcagtc tgctgctttc cgagattttg tatggcgttt
    67921 cgtcaacgtt ttaacgaaaa ccatggaagg tttatccatt aaaccaagct atgaaaatat
    67981 ctataagtct gctgcaaatg tagatgaact tgcaggggat tactttaaaa agatattaga
    68041 taaacaccac cctggttggg agaaagactt cgacaatttt gatatgcccg agtctgaagc
    68101 aaaaagcgca gtcaaaactg ggcgcaactt ggacacaatg aagctcggtt ctttcttgaa
    68161 ggccaaaaag cataacgaac ctcttattga cgcattgggt ggtattttgt ccaatgacag
    68221 atcctatttt gagaagctag tcagctccct gtatccattg ctggaaaaac tgacgactgg
    68281 cgaagttgcc aagcttatca gcccggatac agaggatttg tcagatccaa gaatcattat
    68341 ggactggcgc aaagtcatgg aatcgaatgc cgtcgtctat attggattgg attccctatc
    68401 tgatgccgaa gttgcagcag ccgttggtaa tgcgatgttt gctgacttaa cctctcttgc
    68461 gggtcagatc tataaattcg gccatggggt tggacagagt ggaaaaaccc agaaaagaaa
    68521 agtctcaatt catgcagacg aattcaatga attaatcggt gacgaattca ttccgttatt
    68581 gaataaggcc ggtggtgccg gctaccaagt gacggtttat acacaaacct ggaaagacgt
    68641 agaagccaaa attggttccc ctgctaaagc aggtcaaatt ggcggcaact tgaatacgat
    68701 gattatgctt agggtgaaaa ctgtcgaaac agcagagatg ctgacgaatc agttgccgga
    68761 agtcaaaatt atgaccagca ctctagtctc aagggctggt gatgttgcct tccctgatag
    68821 tcacgaagat tttagcagtg gaaacgaaga caggattggt gctgagacag ttcctatgct
    68881 gacacccgcc gatttaaccc aattacccaa aggtcaagca ttcgcgctta ttgaaggtgg
    68941 ccagctatat aaaattcgac tccctctacc caaaaaggac aagcaggagg aaatccctgc
    69001 gcatattgga gaaatggcgg ctgatatgcg cgaagtttac agactaagcc ggccttcggc
    69061 tgaagaaaac gcatttacgg agggaagtag ttatgccgtc taatacaagc gcatggtaca
    69121 tgaattcatt gcctttaaag ttcttatttc tcccgtttaa agctgcttat accttattct
    69181 taattgtgct gcttatcttt ggtagttcgg tgttgacaca aaactatttt ggtaccggta
    69241 atctcttgga agatgaaatc aaatggtctg agtctgttgg ccatgatgcg tttccaggaa
    69301 aagaggttga cgatactata aatctgcaaa ttgccagact tagttacaag gggctgaaaa
    69361 ccgtattttt tgacctgaca ttagtcaatg aatcactgaa agccaagtca gaagccgatt
    69421 ttggttacaa atttaaaaat tatttagtcc cccgtatgaa atatctacag caattcgata
    69481 ctacgttgaa ggttatttcc ttacgtttgg gaaatttggc attaatcgtg ggtatctcag
    69541 taatctttgc aatcccggtt ttgatagatg ggttagtgat gagagctatc aggcaggaaa
    69601 atgcctcaag ggagagcgcc ggtatttatc accgtgcaaa atattggaga acagggatta
    69661 tttggttggg ttgcatgatt tatatgtgtg tgccaatatc aataccggcc tacttgctct
    69721 atttacctct ggtagcagca atttggatga ttttcatgca agctaaatat cttaagaaat
    69781 atctctaggc ttccagtgga atctaaatag tgctttttta gttcattaaa cgggggctta
    69841 atgctttaac tgacggtaca gaaatctcga aaagcatttt gcaaaatact taaaataaga
    69901 gtatagtaaa aatgaaatat ttcaagttta actaatagtt aacaatctag agtaaacaat
    69961 cgtaatcgtt aaatctgggt atagattgca taatatacat tatgcgaact actggaaaca
    70021 gctgtatgtg ttgcgttgac cttcaatacg atatacaggc agcaggcaca caggatatgc
    70081 cgcccatact tttttaatcc aaaacattat atcaacgggc gcaaacagta aatcgcccaa
    70141 cttatcccaa aaattctcct gctttttcat catttcttct gtcaaatcct tggaacggtc
    70201 gatttgaaaa cacggcctta catacgtccc aattctcttt gcagtgcttg gatattttgc
    70261 tgtctgtcca aaacattgct ttgcaatgcg ttgatttttt gatggttgat gttgccgcct
    70321 tttgccagac gtgcttgtga taacattttt tgggcttcag tcagggcttt gcgttcattg
    70381 cttaattctg cttcgagaat ggagcgtctg ctgttattta caggtgcttg ttgaggcggc
    70441 gtattggatt ttgccggctt ggatactgtt ttgaccgggg ctttatattt gacagcctgt
    70501 ccgccgtttg acggtgatgg tgccggttcg ggagtttggg gcgggatata gcgttcgctg
    70561 ctgtagttgc cgattggggg caaatcggtt gagtggcagc ttttagacgg cttggtggtg
    70621 taaacggttt ctccgttgat tgtgcaggta tagattttgg ccgcattcgc acccaatggg
    70681 cttgaaatca gggaaaggtt gattaggatt aaggggagtt ttgatttcat aatgtgtttg
    70741 atattcggat aataatttga ttttttgagg attttttgta aggttttctt ggcgttttat
    70801 gggcttttct tttcggttgt ttatttttga ttttccttcg gggctttggc agcctgtctg
    70861 ttctttttcc cggcatgttt tttgtttttc tttttgtttc ctgtgtgtat tttggcttct
    70921 tgactaagtt ttttaatttt caggcggtat ccgcctccct gatggctgct gattttaggt
    70981 aaatcagcat cggcacacga tcctgacggg tctgatgtat atacgctttt gccatttgag
    71041 ttgcaatggt atatggaggc ttgcgccgcc gcgccggaaa gggacaggaa acccaaggcg
    71101 gcaaaaagtc tgatgtttat accggtaaag taaaaaggcg gtggtaacgt ccgaattata
    71161 ccgttattgg caggcaaaga taagcaccct gcccgcgctt ctttatcgct cggcaaactg
    71221 tttctcgatt tcagtttcaa tccgttccaa ttcttcttga caagccagcc aagtttcttc
    71281 gatttgggca agctgtgttt tgacttttgc cagctcggat aaggtatttt gcaatttttc
    71341 tttgtttgct tcaaggtaag cgtcttcttg tgttaaaaat gcttcgcatc ccgtctgaat
    71401 ttcagaaagc tgcaccattt ctttttcggc tttgtcgatt ttctgttgta tcggcttact
    71461 gcgtcgggct ttttcttgac ggatttgcgc ttcgatacgc ttggtatcct tgcggttttg
    71521 gctttgagtg gaagcggctg gcgcggttgc ggcgttttcc tgtgccaagc gccattggcg
    71581 gtaatcgttc aaatcgccgt caaagttttt cagacgacct ttgtctatca agaggaaact
    71641 gtcggtcgtg gcttcaagca ggctgcggtc gtgcgatacg acgattaatg caccttggaa
    71701 actttgcaac gcgagcgtta aggcatgacg catatccaaa tccagatggt tggtcggttc
    71761 gtcaagcagc agcaggttcg gcttttgcca gataatcatg gcaagggcaa ggcgggcttt
    71821 ttctccgccg gaaaaaggtt cggttttctg taacgccata tcgccgacaa aatcgaagcc
    71881 gccgaggaaa tttcggattt cttgttcgcg tacttcggga gaaagctgct gtatatgcca
    71941 aacagggctt tggtcggcgc ggatggtgtc aagctggtgt tgggcgaaat agccgatatt
    72001 aagtttttcg gaatggacga tgctgccgga gagtaaatcg atttttcctg ccaaagcttt
    72061 gataaacgta gatttaccgc tgccgttgac acccaatagc ccgtagcgtg cgccgctttc
    72121 cagcgacagg gtaatgtcat gcaggacggt tttaccttcg taacccaaat cagcgtgctc
    72181 cagcttcagc aaaggattgg gcagatggtc gggattgtaa aactcaaagg aaaactcgct
    72241 gtccagatgt gcgggagcga tgcgttcgag cttcgccaac gccttcatgc ggctttgagc
    72301 ttgaacggct ttagtggctt tggctttgaa gcggtcgata aaggattgca gatgtttgat
    72361 ttgcgcctgc tgtttgacat atgcggcttg ttgttgcgcc agacgctgcg cgcgttcggt
    72421 ttggtaaaaa tcgtaattgc cgctgtattg cgtgagcttt tgctgcgaca attcaatggt
    72481 ttgggtggtg gccgcattga ggaaatcacg gtcgtgggaa atgatgattt gcgtgcaggg
    72541 taaagaggca aggtggtttt ccaaccacaa gacagtttcc aaatccaaat ggttggtcgg
    72601 ttcgtcaagc aagagcaaat cagcgcggca aatcagggct tgcgcaagat tcaggcgcat
    72661 acgccagccg ccggaaaagg atttgacggg acggctgtgt tcttcttgcg aaaaacccag
    72721 cccgttcaac aattttgccg cgcgggccgg cgcggtatag gcgtcgattt cttccaattt
    72781 ggcatgatat tccgcctgct tcatgccgtc attttgcact tccgcctgcc tcaatgccgt
    72841 ctgaaaagcc tgcaactcgg catcgccctg caaaacgtaa tccaaagcgg aaatatccaa
    72901 atcgggcgtt tcttgggaaa cggaagcgag ccgccagttt ttcggaatcg agatatcgcc
    72961 gccgtcctga gtgatttcgc ccttgattaa ggcaaacagg ctggatttgc ccgtcccgtt
    73021 cttaccgatc aaaccgacgc gctgaccggg attgacggca gcggaagcct tgtcgagcag
    73081 gactttcaaa ccgcgttgca gggtgaggtt tttgatttca atcataacgg aaacatcgtc
    73141 gggcgggaaa agcccgtatt ttacctgaaa gtcagtgccg atgccgtctg aaacgggaaa
    73201 tttacggttg aagccaagcc caagccctgc gcccttccga gtgcaggaaa accaatgtcc
    73261 tgaatgccga atcggtattc atgcattcca cgctgattcc gattcgggaa aaatccgcca
    73321 tgattttggg atggataaac tcctgagccg cgcccgtccc gataatcaat atttccggat
    73381 agtcaacagg tttgatgtcg gacaacaggt tttccggagt caggtcggac aaggttcggc
    73441 attgcggcag gcagaccgaa tccttatgta caagcacggg tttatggaaa ctttgccccg
    73501 ccagccggat tccgcccgcg ccgcattcat attctgcaaa ctgtccgtct atcggatttt
    73561 cttcaaacaa cattttttac cccgttgctg catcatctgc accgaaaggg gtgcaaaatc
    73621 aggcgaatta atgtaggatt ggcagatttc atctgacgcg cctgccgatt tcagacggca
    73681 tttgattcaa agtgcggcac aattataccg gcagcggata ttttcgtctt tcaatattta
    73741 catttcagtc ggcttacaag gagacacaat gaagccagta aacatcggtc ttttaggctt
    73801 aggtacggtc ggcggcggtg cggctgccgt gttgcgggac aacgcagagg aaatttcccg
    73861 ccgcttgggg cgcgaaatcc gtatttctgc catgtgcgac ttaagcgaag aaaaagcccg
    73921 ccaaatctgc ccgtccgccg cctttgtcaa agatccgttc gaactggtcg cgcgtaaaga
    73981 cgtcgatgtc gtcgtcgaat tgttcggcgg cacgggcatt gccaaagaag cggtgttgaa
    74041 agccattgaa aacggcaaac acatcgttac cgccaacaaa aaactgctcg ccgaatacgg
    74101 caacgaaatc ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg aagcggcagt
    74161 agcgggcggt atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa acaggattaa
    74221 atccatcgcc ggcattatta acggcaccag caacttcatc ctctccgaaa tgcgcgaaaa
    74281 aggcagcgcg tttgccgacg tattgaaaga agcgcaggca ttgggttatg ccgaagccga
    74341 cccgaccttc gacatcgaag gcaacgacgc aggccataaa atcaccatca tgagcgcgct
    74401 ggcattcggt acgccgatga acttctccgc ctgctatctc gaaggcatca gcaaactcga
    74461 cagccgcgac atcaaatacg ccgaagaact cggctaccgc atcaaactgt tgggcatcac
    74521 ccgcaaaacc ggcaaaggca tcgaattgcg cgtccatccg accctgattc ccgaaagccg
    74581 cctcttggca aacgtcgacg gcgtgatgaa cgccgtgcgc gtcaacgcca atatggtcgg
    74641 cgaaacctta tattacggcg cgggcgcggg cgcattgccg accgcttccg ccgtggttgc
    74701 cgatatcatc gacatcgccc gcctggttga agccgacacc gcccaccgcg taccgcatct
    74761 ggcgttccaa cccgcccaag tccaagcgca aaccatcctg cctatggacg aaattaccag
    74821 cagctactac ctgcgcgtcc aagccaaaga cgaaccgggc acgctggggc aaatcgccgc
    74881 gctgttggca caagaaaacg tgtccatcga agccttaatc caaaaaggcg ttatcgatca
    74941 gaccactgcc gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca tcaagtcggc
    75001 aatcgcagcc atcgaagcac tggattgtgt ggaaaaaccg attaccatga tccgcatgga
    75061 aagcctgcat gactgagccg aaacacgaaa cgccgacgga agagcaggtt gccgcgcgca
    75121 aaaaagcaaa agccaaaatc cgcaccatcc gcatttgggc gtgggtcatt ttggcgttgc
    75181 tcgcttcaac cgccctgctc tcccaatgcg cgatgtccaa accgcaggca aaacagaaaa
    75241 ttgtcgagtc ttgcatgaaa aatattccgt ttgctgaaaa atggcagaac gatttgaaag
    75301 cgcgcggctt ggatgcggac aatacccgtc tcgccgtcga ctactgcaaa tgtatgtggg
    75361 agcagccttt ggacggattg agcgagaaac agatcagctc cttcggcaaa ctcggtgcac
    75421 aagaacagct tgacctgctc ggcggcgcaa acgcgtttga aactcgagac aaacaatgtg
    75481 tcgcggattt gaaagccgat tgaacctcag gtctggaatg ccgtctgaac gcggggaaaa
    75541 gcgtttcaga tggcattttt ccatccgggt ttgacgggca gcccttcatc cgaatccgac
    75601 gggcaaaccc ctatcctgat tcggcgggga aaacaaaaag ccgattcttc aaggaatcgg
    75661 cttttgctga aaaacagttt acagtgcgtc tttcaatgct ttacctgcac ggaatttaag
    75721 cgttttggcg gcggcaatgg tcagaggctc gccggttttg gggttgcggc cttggcgttc
    75781 cgcacgttcg ccgacgtaga aagtaccgaa accgaccaga gtaacggtgt cgccttgttt
    75841 cagggcgttg gttactgcgt tggtagtggc atccaaagct ttttgtgcgg cggctttgga
    75901 aatatcggct tcttgagcaa tcgcttcgat caattcagac ttgttcacaa tcagtccctt
    75961 cctgtcttaa aaaatgatga aatgcccgaa tactcggggt ttgtactgct tgagcaactt
    76021 tcgctttata gcaattctga aattgccgtg tcaagcaaaa aatacggaat cgccctattt
    76081 tacaggcttt caggacgaaa ccgcattttt acaacacatt tcctgcgttt caatgtttga
    76141 ttgccctgct gcggggtttc ggttttgaag cggattccgc cgccgcttcc gcggcagaag
    76201 gttctgccca agactcaggc cggctttcca aacccaaagc caatacctcg tctatccatt
    76261 tgaccggatg gatggttaat ccggttttca cgttttcagg gatttcttcc aggtctttga
    76321 cattgtcttt cggaatcagg acgtgtttga tgccgccgcg aagcgcagcc aacagttttt
    76381 ccttcagacc gccgatcggc aggacttcgc cgcgcagggt aatttcgccc gtcatggcaa
    76441 catcggcacg caccggaatt ttggtaaagg cggacaccat cgccaaagtc atagcaatgc
    76501 ctgcgctcgg accgtctttc ggcgttgcac cttcgggaac atggacatga atgtcttttt
    76561 tctcgtaaaa atcaggagcc aaacctactg attccgcacg ggaacggaca acagaccatg
    76621 cagcggatac agattccttc atcacatcgc ccaactggcc ggtacactgg atcatgcctt
    76681 tacccggtaa tgctgcggct tcgacagtca gcaattcgcc gccgacttcc gtccaagcca
    76741 aaccggtaac ctgtccgatg cggttttcac tttcggcaac gccgtaatcg aagcggcgca
    76801 cgcccaagta gtcatgtagg tttttctcat tgactttaac cgccctaggc ttggctttgc
    76861 tggttttctt ggtttcagac aacctcttct tatcttcgtt caaggtaatc tgcatcacca
    76921 ccttacggca gattttggca atttcgcggt cgagcgaacg cacgcccgcc tctcgggtgt
    76981 aataacggat aatatcgcgt accgcgcttt cttcgaccac caattcgcct tctttcacgc
    77041 cgttgcgttt catttgcttc ggtacgagat actgcatcgc gatattgatt ttctcgtctt
    77101 cggtatagcc ggacagacgg atgatttcca tacggtcaag cagcggggtc gggatattca
    77161 ggctgtttga agtcgcaata aacatcacgt cgctcaaatc gtaatccact tcggcataat
    77221 gatcggcgaa cttgttgttt tgttcgtgat cgagcacttc aagcaacgcg ctggcgggat
    77281 cgccccgaaa gtcgctgccc aatttgtcga tttcgtcgag caggaacaac gggtttctta
    77341 cgcctgcttt tgccatattc tgcaaaatct tgccgggcat agagccgata taggtacggc
    77401 ggtgcccgcg gatttcgctt tcgtcgcgca cgccgcccaa ggccatacgg acatatttcc
    77461 gccccgttgc tttggcgatg gattcgccca aagaggtttt gcccacgccc ggagggccga
    77521 ccaaacacag aatcggacct ttgagcttgt ccatacgttt ttggacggcg aggtattcca
    77581 aaatccgttc tttgactttt tccaggccgt agtggtcggc atccagcacc agtccggctt
    77641 tggcgatgtc tttgctgacg cgggattttt tcttccacgg cagtccgagc agggtgtcga
    77701 tgtagttgcg tacgacggtg gattcggcag acatcggcgg catcattttg agttttttca
    77761 gttcggacag gcatttttct tccgcttctt tggtcatacc cgcctttttg atgcctgcct
    77821 ccaaggcatc cagttcgccg ttttcgtctt cttcgcccaa ttctttgtgt atcgctttaa
    77881 tctgttcgtt cagataatat tcgcgttggg atttttccat ttggcgtttg acgcgtccgc
    77941 gtatgcgttt ttcggcctgc ataatgtcga gttcggattc cagctttgcc agcaggaatt
    78001 ccatccgttt gccgatttcg ggaatctcca aaatctgttg gcgttgcgcc agtttcaact
    78061 gcaaatgcgc tgcgaccgta tcggttagcc ggctgttttc ggcaatgccg ttgatgctgc
    78121 cgataatttc ggcagggatt tttttattga gtttggcgta ttgttcaaac tgcgccaaca
    78181 gggtgcggcg cacggcttcg aggtcggtat tgccgcccgt gtcttcttcc acgaccgcct
    78241 ctatatgaga aacgaacagc ccgcccgtgt cttcgatggt caaaacacgt ccgcgataca
    78301 gcccttcgac cagcactttt accgtgccgt cgggcagttt caacacttgc aggacttgtg
    78361 cgacggtacc ggtctgatac aggtcggcgg caaccggttc ttctaccgcc gcatcggttt
    78421 gcgccaacag gaaaaccggt tcctcgcggg taatggcgtt ttccagtgcg gcgatggatt
    78481 tcggcctgcc gacgaacagc ggcagaacca tatgcgggta aacgacgaca tcccgtaaag
    78541 gaagggtcgc caaagcagca tattcctcaa aatgcttttc tttttgtgtc atggatactc
    78601 tcttgtgtct gacaggatac ggattgccgc gtaaattggg gttgaaagta ttatttcaag
    78661 catacgcggt ttatttatgg agtttgatgc catgccgtct gaaacattcc ggcttcagac
    78721 ggcatgggct tggaaagaca aggcgggaac aaaaaaacag ttctgtgttg ccgctccttg
    78781 ctgtaccatc cgtatggttt gcggttctgc cgccctattt tcaaaacatg acgcaggtat
    78841 cccatgtctt cttttatttt tcccgataac gggttcgaat ggcgcaacga aagtctgcaa
    78901 aagccgccca aaggttggta ttatgtccgc gaaagcggtg cagacggcat tttgaaggct
    78961 acctatcaaa atattgcaac tgtctggcag ggcgatttcc acaatgccaa acaggtgctt
    79021 tcggcaatga agaagaaggt ccgcaaacct gccgccgtcc gttccgatgc ggatatcgcc
    79081 gcccttttcc acgcccaccg tatgaagcag gcgcaacaga gccgtattct gaatatgctt
    79141 gccgttgaaa tccgccccgg tttcgtgttg gacaacaaac gcgcgcccga tatacgcgcc
    79201 gctttgctcg acgtgtacgg agaggcggac ggcaaaccgt tttttctgcc gctcaatctg
    79261 ctgctggggt ttatgggtgt gcatgagtgg cataaga
//
LOCUS       DAWTCI010000003        62699 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWTCI010000003 DAWTCI010000000
VERSION     DAWTCI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 62699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62699
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.003"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(200..1039)
                     /locus_tag="WM481_000230"
     CDS             complement(200..1039)
                     /locus_tag="WM481_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HFA9079651.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1324..1399
                     /locus_tag="WM481_000231"
     tRNA            1324..1399
                     /locus_tag="WM481_000231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1357..1359,aa:Arg,seq:ccg)
     gene            complement(1464..2657)
                     /locus_tag="WM481_000232"
     CDS             complement(1464..2657)
                     /locus_tag="WM481_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9079652.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGRMP
                     VLESVSRAETARTAVPAPSPYLPMEGLDVYRSAVQHLLFGKGNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSERQWDEVLQIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMDLPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSSELYALWQNEVYMMRDRIRAMRQKLYGVLTARIP
                     DRDFTYFIKQRGMFGYTGLSVGQVRRLRDEFAVYLLDSGRMCVAGLNTSNITYVADAL
                     AEVLK"
     gene            complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WM481_000233"
     CDS             complement(2677..3765)
                     /gene="trmA"
                     /locus_tag="WM481_000233"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFA9079653.1"
                     /translation="MNDYTQQLQGKKDYLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKASGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNHW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMKAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLRVGDRDFHYRQIEGSFTQPNAAVCRKMLEWACRAAEGLGGDLLELYCGNGNFTL
                     PLSRYFRQVLATEISKTSVSAAQWNIEANWIGNIKIARLSAEEFTEAYTGKREFTRLK
                     ESGIVLTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLTETH
                     TVGRAALFDQFPFTHHIESGVLLKKKIL"
     gene            3912..5012
                     /gene="aroC"
                     /locus_tag="WM481_000234"
     CDS             3912..5012
                     /gene="aroC"
                     /locus_tag="WM481_000234"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFA9079654.1"
                     /translation="MAGNTFGQIFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATAF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKKIRFEGSEHISQNPFFAANQSQIAELEHYMDGVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDIHGNPVELATRGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAADTPDISRSDK"
     gene            5103..5528
                     /locus_tag="WM481_000235"
     CDS             5103..5528
                     /locus_tag="WM481_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HFA9079655.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5602..7587)
                     /gene="parE"
                     /locus_tag="WM481_000236"
     CDS             complement(5602..7587)
                     /gene="parE"
                     /locus_tag="WM481_000236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFA9079656.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATEIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVSVKRDGKVHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTSLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARIRSVKKIEKKKGSGVAILPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHAINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAQLDI"
     gene            complement(7654..8178)
                     /locus_tag="WM481_000237"
     CDS             complement(7654..8178)
                     /locus_tag="WM481_000237"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFA9079657.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8344..9639
                     /gene="serS"
                     /locus_tag="WM481_000238"
     CDS             8344..9639
                     /gene="serS"
                     /locus_tag="WM481_000238"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFA9079658.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPPGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYGALEEMVGHAENILKALELPYRVITLCTGDMG
                     FSAAKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVTKLEVK"
     gene            complement(9712..10710)
                     /locus_tag="WM481_000239"
     CDS             complement(9712..10710)
                     /locus_tag="WM481_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFA9079659.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGLELEFFDFMLDAKTAKMAEG
                     AEAACIFVNDDASRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEVEKLGGRYVDLDELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKCRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WM481_000240"
     CDS             complement(10835..11911)
                     /gene="prfA"
                     /locus_tag="WM481_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFA9079660.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIDELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGGEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRTDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREVQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(12001..12771)
                     /locus_tag="WM481_000241"
     CDS             complement(12001..12771)
                     /locus_tag="WM481_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFA9079661.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATCPQDW
                     QDVAELSEMPSEHGQIRIALGIHPWFSDGIAEQDFSGLEAVLVRYPRAWVGEIGLDFY
                     DKTQTPPQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFTQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLENTV
                     NTPANILRIAEIAAEIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12774..13766)
                     /locus_tag="WM481_000242"
     CDS             complement(12774..13766)
                     /locus_tag="WM481_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFA9079662.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSAGLRPDTALVGQALAPFSDGMDFD
                     WHVCEPLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANMFALALQGL
                     DKPIVLTGSQWPYNAEGSDAPRNLVTAVAAFPLGLKQTLIAFDGKLYPAVGSSKISTE
                     TAAGFGNAHFGALAEWDETRGWHNVRIPNQDAADVSDGLEIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTSAGEGFIRAVQDFTQQGKLLLNISQVPQGC
                     AAAVYAQGDALRRAGVVNGGKCNLETATALMTLAVSEGWGADGVKHELQRLGLV"
     gene            complement(13833..14516)
                     /locus_tag="WM481_000243"
     CDS             complement(13833..14516)
                     /locus_tag="WM481_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9079663.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="WM481_000244"
     CDS             complement(14705..16039)
                     /gene="glmM"
                     /locus_tag="WM481_000244"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFA9079664.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNTYSDNGIKFFAEGGVKLSDEVELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPGHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGNEPNGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNRQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQILNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIAAAIGGI"
     gene            complement(16169..17020)
                     /gene="folP"
                     /locus_tag="WM481_000245"
     CDS             complement(16169..17020)
                     /gene="folP"
                     /locus_tag="WM481_000245"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFA9079665.1"
                     /translation="MARHVWRAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRSGADFVPPEEEWARVEPVLAEAAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMRGLPETMQDNPKYQ
                     DVVGEVARYLKTRSETCVAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGKLTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKVW
                     EALGVNR"
     gene            complement(17160..18053)
                     /locus_tag="WM481_000246"
     CDS             complement(17160..18053)
                     /locus_tag="WM481_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFA9079666.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWSNADAVFLDPFFTSANLYDTLSADEGRGRCRRLAEQAASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLGHTKGHPYRLDVESIVKVRQISHLPVIANITGLWSRDMPQEILY
                     GLAKAAGACGIVGTCFEKAGG"
     gene            18144..20246
                     /locus_tag="WM481_000247"
     CDS             18144..20246
                     /locus_tag="WM481_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFA9079667.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIRRRLLPRPTVILKNLTITEPDGGRVAVSVKETKIGLSWK
                     NLWSDRIQVEKWVVSGADLALTRDRNGAWNIQDLFDGAKHSASVNRIIVENSTVRLNF
                     LQQQLILKEISLNLQSPDSSGQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPALALK
                     NNSIKTGTVNGTFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRLQTNF
                     SLGSPLVWSRDNGLDAPRLHISTLQDTVDRLPQPRFISRLDGSLSIPNLQNWNAELNG
                     TFDRQPVAAKFKYTREGAPHLEAAAALQKLNLAPYLDEFRQQNGKIFPDILGRLSGNV
                     EAHLKIGSIQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGENRKQLIRSLQGSLSLNISN
                     GAWHGIDMDSILKNGLSGKISGSTPFYRFTLNSEISDGISRHIDTELFSDSLYVTSNG
                     YTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRKEKQK
                     ILEDTLLEQWQWLKPKEP"
     gene            complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="WM481_000248"
     CDS             complement(20310..21788)
                     /gene="ubiD"
                     /locus_tag="WM481_000248"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFA9079668.1"
                     /translation="MKYKDLRDFIAMLEQEGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLVMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPSTILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGSDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQGHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFSEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMTGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATSKWPGETDREWGRVIKKDPAVTVKIDGIWGKLGL"
     gene            complement(21808..22323)
                     /locus_tag="WM481_000249"
     CDS             complement(21808..22323)
                     /locus_tag="WM481_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFA9079669.1"
                     /translation="MHTCTSCGEKHEDMPATGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYSGQTDRFIRAALPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQSDNQRPLLQLHQSGGHPPVRDFYHGMGYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22401..22698)
                     /locus_tag="WM481_000250"
                     /pseudo
     CDS             complement(22401..22698)
                     /locus_tag="WM481_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22695..22802
                     /locus_tag="WM481_000251"
                     /pseudo
     CDS             22695..22802
                     /locus_tag="WM481_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(22960..23772)
                     /locus_tag="WM481_000252"
                     /pseudo
     CDS             complement(22960..23772)
                     /locus_tag="WM481_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23972..24190
                     /locus_tag="WM481_000253"
                     /pseudo
     CDS             23972..24190
                     /locus_tag="WM481_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702943.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24502..25167
                     /gene="fabG"
                     /locus_tag="WM481_000254"
     CDS             24502..25167
                     /gene="fabG"
                     /locus_tag="WM481_000254"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9079670.1"
                     /translation="MADDGFDIAVHCRSRRDEAEAVAEEIRALGRNARVLQFDVSDRE
                     ACREILTADIEANGAYYGVVLNAGLTRDNAFPAFSDDDWDVVPRTNLDGFYNVLHPLV
                     MPMIRRRKAGRIVCMASVSGLTGNRGQVNYSASKAGIIGAAKALAVELAKRKITVNCV
                     APGLIDTEIVDENVPVGEILKAVPAARMGLPEEVAHAVHFLMDEKAAYITRQVIAVNG
                     GLC"
     gene            25161..25979
                     /locus_tag="WM481_000255"
                     /pseudo
     CDS             25161..25979
                     /locus_tag="WM481_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; frameshifted; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            25956..26506
                     /locus_tag="WM481_000256"
                     /pseudo
     CDS             25956..26506
                     /locus_tag="WM481_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
     gene            26560..26635
                     /locus_tag="WM481_000257"
     tRNA            26560..26635
                     /locus_tag="WM481_000257"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26593..26595,aa:Ile,seq:cat)
     gene            26710..27468
                     /locus_tag="WM481_000258"
     CDS             26710..27468
                     /locus_tag="WM481_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9079671.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSADNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVRTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGGSVQGKVHETVQTPYPERRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVSFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            27469..28533
                     /locus_tag="WM481_000259"
     CDS             27469..28533
                     /locus_tag="WM481_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9079672.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     DELMRRRFSEVSSVFTTRLHTFNGLFSLYALTRFIRENHISHLMIHTGKIAALSVLLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRGVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVAARFTINHTADKLLDAI"
     gene            28767..30104
                     /locus_tag="WM481_000260"
     CDS             28767..30104
                     /locus_tag="WM481_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9079673.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAVRSLTLPDAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PNQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGAWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLGIL"
     gene            30171..30275
                     /locus_tag="WM481_000261"
                     /pseudo
     CDS             30171..30275
                     /locus_tag="WM481_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(30279..30878)
                     /locus_tag="WM481_000262"
     CDS             complement(30279..30878)
                     /locus_tag="WM481_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9079674.1"
                     /translation="MWKLYADYFAGRPDAETPPENSVKETLKRVGFGKVSFDDREIRF
                     AEKGMGLSLNGIAQGYITDKVVALLKANGVPAALVDMGEIRGFDTNGRRMWNVGIRNP
                     DDEEGVLANITMKDKAFATSGGYGTVMDKAGRLTHLFDPRTGVSTPRYKSMSVMADDA
                     AVADALSTAFSVMDLPLIRSVAESRRLKVRLAMPDNIVD"
     gene            complement(30911..31291)
                     /locus_tag="WM481_000263"
     CDS             complement(30911..31291)
                     /locus_tag="WM481_000263"
                     /inference="COORDINATES: protein motif:HMM:NF014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9079675.1"
                     /translation="MGRHFGRRRFLTAAAVAVAGAAVSFLPNPFAAGGEKRNMDKKRD
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLAAIFTR"
     gene            complement(31300..32094)
                     /locus_tag="WM481_000264"
     CDS             complement(31300..32094)
                     /locus_tag="WM481_000264"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFA9079676.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRSWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAVVSV"
     gene            32518..33852
                     /gene="gdhA"
                     /locus_tag="WM481_000265"
     CDS             32518..33852
                     /gene="gdhA"
                     /locus_tag="WM481_000265"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFA9079677.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFTSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSDGFVLFPDIGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCHVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWA
                     REEVDSRLFGIMQSIHESCLKYGKVGDKVNYVNGANIAGFVKVADAMLAQGF"
     gene            33947..34053
                     /locus_tag="WM481_000266"
                     /pseudo
     CDS             33947..34053
                     /locus_tag="WM481_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(34091..34870)
                     /locus_tag="WM481_000267"
     CDS             complement(34091..34870)
                     /locus_tag="WM481_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HFA9079678.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNILVARQILEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIRFWLEKFEEACGSGNLEHQGEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            35148..35447
                     /locus_tag="WM481_000268"
     CDS             35148..35447
                     /locus_tag="WM481_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9079679.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPTGMAVSSLSPQPD"
     gene            35432..35621
                     /locus_tag="WM481_000269"
                     /pseudo
     CDS             35432..35621
                     /locus_tag="WM481_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(35733..37136)
                     /gene="mtrE"
                     /locus_tag="WM481_000270"
     CDS             complement(35733..37136)
                     /gene="mtrE"
                     /locus_tag="WM481_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFA9079680.1"
                     /translation="MDTILKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANGSRQGSLSGGNVSSSYNVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVGTGSAELGGLFKSGTGVWS
                     FAPSIALPIFTWGTNKANLDAAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     AALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTGK"
     gene            complement(37192..40395)
                     /gene="mtrD"
                     /locus_tag="WM481_000271"
     CDS             complement(37192..40395)
                     /gene="mtrD"
                     /locus_tag="WM481_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFA9079681.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPNIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPETDEDL
                     AQVEVQNKLSEVLSMLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNIQISAGSIG
                     SLPAVQGQTVTATVTAQGQLSTAEEFGNVILRANTDGSNVYLKDVAKVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLLEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKAATKKAMGQISGAVIGITAVLMSV
                     FVPLAMFSGATGNIYKQFALTMAASIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFDNWTHGYEGWVAKVLRKTLRMMVVYVGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATKERTDATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     MLKDWNERKTPGSDAESVAGKLTGMMMGTLKDGFGIAIVPPPIMELGNGSGLSINLQD
                     RNNTGHAALLAKRNELIQKMRTSGLFNPSTVRAGGLEDAPQLKIDINRAAAAAQGVSF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADGDARMQPADILNLTVPNSSGIAVPL
                     SSIATVSWQMGTEQSVRFNGYPAMELSGSPATGVSTGQAMEAVQKMVDELGSGYSLEW
                     GGQSREEAKGGSQTIALYALAAVAVFLVLAALYESWSIPLAVLLVMPLGLAGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(40407..41645)
                     /gene="mtrC"
                     /locus_tag="WM481_000272"
     CDS             complement(40407..41645)
                     /gene="mtrC"
                     /locus_tag="WM481_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFA9079682.1"
                     /translation="MAFYASKAMRAAALAASVALALSSCGKADEAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEASLESARAQLATAQATLAKADADLARYKPLVAADAISKQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVDE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGTKDTVMIVNAQ
                     GGMEPREVTIAQQQGANWIVTSGLKDGDKVVVEGISIAGMTGAKKVTPKEWMPSENQA
                     AAPQSGVQTASEAKPASEAE"
     gene            41893..42525
                     /gene="mtrR"
                     /locus_tag="WM481_000273"
     CDS             41893..42525
                     /gene="mtrR"
                     /locus_tag="WM481_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFA9079683.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAANAEGGSWTVFRHTLLHFFERLQSN
                     DIHYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSGENFDLGKTAPRIIGIIMDNLENHPCLRRK"
     gene            complement(42618..42830)
                     /locus_tag="WM481_000274"
     CDS             complement(42618..42830)
                     /locus_tag="WM481_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079684.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQDNVHAMIGEIKNAI"
     gene            43162..44730
                     /gene="mtrF"
                     /locus_tag="WM481_000275"
     CDS             43162..44730
                     /gene="mtrF"
                     /locus_tag="WM481_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFA9079685.1"
                     /translation="MSQTDARRSGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVTSTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVAGSPFLKSIVVFIFLLFALPGIVYGRIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVG
                     LAGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVMKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            complement(44800..48009)
                     /gene="recC"
                     /locus_tag="WM481_000276"
     CDS             complement(44800..48009)
                     /gene="recC"
                     /locus_tag="WM481_000276"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFA9079686.1"
                     /translation="MFYLYQSNRLESLAALFARIQKVKPLKCALQPEQIVVQSQGMRR
                     YLNTCLARDLGVAANLAFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSA
                     EFQNGAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMHWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIEPYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPFFYALECVSDLLESRFEVGKVLALLETAPVLRRFGLTGDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAADRMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLSDIARIWRQPATVGEWVARCRDLLETLFQAGPDDQKAVQNLE
                     NEWVKWQEESTLAGFFGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLVAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPRPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFDPQHADQIAETYIEARREGQDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPFPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRVAFLLEHLIFCAVMPSEAETRQTFIVQSGETEVLAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGTGEA"
     gene            complement(48110..49519)
                     /locus_tag="WM481_000277"
     CDS             complement(48110..49519)
                     /locus_tag="WM481_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9079687.1"
                     /translation="MDTQIKTEAGQQNNRHYLSVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVFPQAVVQPLSVQAEAARGAINPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHGDMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWAKQRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWILELTPMPVS
                     GTTVGENGINPSEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVVFCLAV
                     IFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRKWFK"
     gene            complement(49863..51182)
                     /gene="ccoP"
                     /locus_tag="WM481_000278"
     CDS             complement(49863..51182)
                     /gene="ccoP"
                     /locus_tag="WM481_000278"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HFA9079688.1"
                     /translation="MNATSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPKGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSNKDGKAPVKKAEPAPAAEPAPSAPAEAAQ
                     AASEAKPAAAEPKAEEKAAPAAKADGKQVYETVCAACHGNAIPGIPHVGTKADWADRI
                     KKGKDTLHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            complement(51206..51376)
                     /locus_tag="WM481_000279"
     CDS             complement(51206..51376)
                     /locus_tag="WM481_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFA9079689.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDDAANSIFAE
                     NQDAQDKKSENR"
     gene            complement(51381..51992)
                     /gene="ccoO"
                     /locus_tag="WM481_000280"
     CDS             complement(51381..51992)
                     /gene="ccoO"
                     /locus_tag="WM481_000280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFA9079690.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            complement(52019..53452)
                     /gene="ccoN"
                     /locus_tag="WM481_000281"
     CDS             complement(52019..53452)
                     /gene="ccoN"
                     /locus_tag="WM481_000281"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFA9079691.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKVKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYMIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            53778..55526
                     /locus_tag="WM481_000282"
     CDS             53778..55526
                     /locus_tag="WM481_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFA9079692.1"
                     /translation="MIHYKIAPSPLDHEWHILLKFTQDNDFPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHTAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPPEWRIATTLPETGRLVFQTASYDELIDRP
                     VEMGLIEFLDFEAAGIPHTIALNGIYPDFDRDRLVSDIKKICETELAMFSSPAPFEKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDAYTTLLGLFSHEYFHAWNV
                     KSIKPAAFAPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPEFYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLTDFFQTALYSTEDLPLAEC
                     LATTGVKLTFLPLPRQHGGGYAEHIFPIPPTGDFGARFKQNADHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFAAQWARYHVRAKINIHFFRAGILRQTVLTVQATAADTA
                     ILHITDRNLLENWLFG"
     gene            55591..56019
                     /locus_tag="WM481_000283"
     CDS             55591..56019
                     /locus_tag="WM481_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HFA9079693.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(56520..56954)
                     /locus_tag="WM481_000284"
     CDS             complement(56520..56954)
                     /locus_tag="WM481_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HFA9079694.1"
                     /translation="MAFGSMNSSDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQANKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(56957..57619)
                     /gene="exbB"
                     /locus_tag="WM481_000285"
     CDS             complement(56957..57619)
                     /gene="exbB"
                     /locus_tag="WM481_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFA9079695.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSTVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(57685..58536)
                     /locus_tag="WM481_000286"
     CDS             complement(57685..58536)
                     /locus_tag="WM481_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFA9079696.1"
                     /translation="MDKERILTPAVVFSVALLHLAIVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGGGAPEGAGAPAAPEPQPAPDPPKPVEPPKPVLKPAVTKKADADIQQPKEK
                     PKPEEKPKPEPEPEAKPAPKPAEKPAEKPSEKPAEHSGNASAKAGSEQGNGEGKGTGT
                     KGDGTGRGEGSGKGSGGAKGEHGEGAGGSGGGTGVGSSKGNPLRANGSIPRPAYPALS
                     MENDEQGMVVLSVLVSPGGHVESVKVVKSSGFSRLDNAARKAAQNGHFQANAWTEFKV
                     PVKFELN"
     gene            58792..59430
                     /locus_tag="WM481_000287"
     CDS             58792..59430
                     /locus_tag="WM481_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFA9079697.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCIAASGP
                     LSNLAMAVLWGVVLVLTPYAGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLPAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(59502..60143)
                     /locus_tag="WM481_000288"
     CDS             complement(59502..60143)
                     /locus_tag="WM481_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFA9079698.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEVVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVCHFDREGRLKDEVRPEIQAWLDKVGGYNNKLVQPRL
                     IKIGLPEFVTPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGTSD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
     gene            60286..62499
                     /locus_tag="WM481_000289"
     CDS             60286..62499
                     /locus_tag="WM481_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9079699.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLA
                     QEHYPADAATPYGEPLPDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATSEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTR
                     ERGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRL"
BASE COUNT        14720 a        17739 c        15822 g        14418 t
ORIGIN      
        1 caaatgggtt aaaccagtat ccatacatca gcttttttat catcctactt tttattcatc
       61 cgatcgggca gacagatttc aaagatgaaa agcctattct attcacaccc atttgatgtc
      121 atttccacac ggacgaacaa ataaattccc ataaaaaaac ggagcagata cctgccccgt
      181 tttttaattt aatccgaatt tagaattttg caccggattg gtttgccata tagtcaacag
      241 ccgctttgac ttcgtcatcg ctcaaacctg cattgccgcc tttggccggc atcgcgttaa
      301 agccttcaag ggcatgtttg tgcaaggttt ctttgccttt tttgatacgc ggtgcccaat
      361 cgtctttttt gcctatgccg ggaataccgg gaatcgaacc gccgtggcac acctgacagg
      421 ttgcttcgaa gactttttta ccgtcaacgc cgaccgcagg ggctgccgca cccttgtctt
      481 ctgccttcgc ttctgccgga gctgcactat cggcaggagc agaagctgtt cctgaagcgg
      541 cattgtcggc aggcgcagcc tcatcaggat tcgggaaaga accgccgctt ttattcgcca
      601 tgtaggtaat cgcccgtttg agttcctgat cggtcaggtc tgccgcaccg cctttggcag
      661 gcatggcgtt aaagccgttc agcgcgtgtt ggaacaaggt atcgaagcct tgcgcgatac
      721 gcggcgccca gtcgccgttg tgttccagtt tcggagcgtt cggcacattg ctgtccgccg
      781 cgtggcattg gatacagatt ttgccgaaaa tctgttcgcc ttggcgttcg ccgacgggga
      841 tgccgtcacc catcgtcaat tgtccgacag gctggatgcg ggtctgcgtt gccgcttccg
      901 tagtggcatc gacatcgccg aacgatccac tgcccgccag cttaatcagg aaataaagga
      961 ctgcaataac aataacgata ccgctcacaa gggtaaacag tgcagagcct tgggctttgt
     1021 tgtcgcggag ttgtttcatt tggtaggcct cgccgtcagg ttaggttgtg ctgtagatta
     1081 tagtttggcg tgttaaacac agttaacaat attttgctgg attatactga attcacatgg
     1141 tctttccaat cgctatcatt gaaaatatga aaaaatttgc caatggtatc tgtacaaaac
     1201 aaataatccg ttgaaaatac cggattatct ccaagaaaac tccccttgtg ccgccatacg
     1261 ccgcctgccg gcgcaagata acctttgcca atttgcagaa tttacgttaa ccttacgttt
     1321 tccgcaccca tagctcagtt ggaagagtgt cagtttccga agctggaggt cacaggttcg
     1381 atccctgttg ggtgcgccaa ttataaagag gccgtctgaa agataaatat ttttcagacg
     1441 gccttttgac ttacttcaaa ttcttatttc aacacttcgg caagcgcatc ggcgacataa
     1501 gtgatatttg atgtattcag cccggcgacg cacatcctgc cggaatccag caggtaaacg
     1561 gcaaattcgt cgcgcaacct gcggacttgc cccacgctca accccgtgta gccgaacatt
     1621 ccgcgctgtt tgatgaaata agtgaaatcg cggtcgggga ttcgggcggt caatacgccg
     1681 taaagtttct gccgcatcgc acggatgcgg tcgcgcatca tataaacttc gttttgccac
     1741 aaggcgtaaa gttcggagct gttcatcacg tcggcggcga tatacgcgcc gtgcgcgggc
     1801 gggctggagt agatgcggcg gacggtgaat ttgagttgtc cgaacaccaa atccgcttct
     1861 tctttattgg ggcaaaccac gcttaagccg ccgacgcgtt cgccgtagag cgacaggttt
     1921 tttgaaaacg aattgctgac aaacagcggc aaatccattt ccaccgcttt gcggacggcg
     1981 taggcatcgc tgtccaaatc gccgccgaag ccttggtagg cgatgtccat aaacggaatc
     2041 agtttgcgcg ttttgatgat ctgcaacact tcgtcccatt gccgttccga catatccacg
     2101 ccggtcgggt tgtggcagca gggatggagg atgaggacgc tgttttcggg cagggtgttg
     2161 aaaaacgcgg tcatttcgtc gaatttcacg ccgacagtgg cagggtcgta atatgggtaa
     2221 gtgccgacct cgaaacctgc gccttcaaaa atgccgcgat ggttgtccca agtcggatcg
     2281 ctgacgtagg cgcgcgcttc gggaaaccag cggtgcagga aatccgcccc gactttgagc
     2341 gcgcccgagc cgcccagagt ctgtacggta acgatgcgtc cctgcgcgag cgcggggttg
     2401 cctttgccga acaacaaatg ctgcaccgcg ctgcggtaaa cgtccaaacc ttccatcggc
     2461 aggtagggcg acggcgcagg cacagcggtg cgggcggttt cggcacggct tacggattcc
     2521 aatacgggca ttctgccttc gtcgtcgaaa taaatgccta tgctcaaatt gactttttcg
     2581 gggcgcgggt cgtttttgaa ggtttcgacc aaactcaaaa tcgggtcgcc gggatagtat
     2641 tcgatgtgtc ggtacatagt ccttaccttt tgcttttcaa aggattttct ttttcaacag
     2701 tacgccgctt tcgatatggt gcgtaaacgg gaattggtcg aacagggcgg cacgtccgac
     2761 cgtatgggtt tccgtcaagg tgtccaaatt ggcgcgcagc gtttcgggat tgcaggaaat
     2821 gtagatgatg ttgtcgaact gcgacaccag cttcaaagtt tcctcatcga tgccggcacg
     2881 cggcggatcg acgaaaatag tggaaaatgc gtaatccgtc aaaacaatac cgctctcctt
     2941 aaggcgtgta aactcacgtt ttccggtata ggcttcggta aactcctcag cagacaggcg
     3001 ggcgattttg atgttgccta tccagtttgc ctcaatattc cattgcgccg cactgacgga
     3061 ggttttggag atttcggttg ccaaaacctg tcggaaatat cgggacagcg gcagggtgaa
     3121 attgccgttg ccgcaataca gttcgagcag gtcgccgccc aagccttccg ccgcgcggca
     3181 cgcccattcg agcattttcc ggcatacagc ggcattcggc tgggtaaaac tgccttcgat
     3241 ttggcggtag tggaaatcgc ggtcgccgac gcgcagggtt tccgtcacat agtcctgttt
     3301 taagactatt ttctgccccc tgctccgccc gataacggaa atatccaact gttgctgtaa
     3361 cgcttgcgcc gccttcatcc actcatcatc aagccttttg tggtaaatca tggtaaccag
     3421 catttccccg ctgagcgtgg acagaaattc gacggcatac caatggtttc tcagttcggc
     3481 agattgggcg gcaacggcaa tcaattcggg catcaggcag ttgactgcct ctgaagcagc
     3541 atcgaaacgg tcgcaacgga tcaggcttgc cccgctagcc ttctgccctt tttcaaacat
     3601 ggcgtaaaac atttcaccgc cttcgtgcca aatgcggaat tcggcgcgca tgcggtagtg
     3661 tttgtccgga gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt
     3721 aaggtagtct tttttacctt gaagctgctg cgtgtagtca ttcatatcgc tatcctgaaa
     3781 aatcaggccg gattataagc cgattttcct cgccgcacgc aaactctttg actgccgccc
     3841 cttcaatgac ggacgggctt ttgtgctaaa atccgccatc tttccacact ataccgataa
     3901 agggaaaaat catggcaggc aacactttcg gacaaatctt caccgtgacc accttcggcg
     3961 aaagccacgg cgcgggtttg ggctgcatca tcgacggctg cccgcccggc ttggaattaa
     4021 gcgaagcgga tatccaattt gacctcgacc ggcgcaaacc cggcaccagc cgccacgtta
     4081 cccaacgccg cgaagccgac caagtcgaaa tcctctccgg cgtattcgaa ggcaaaacca
     4141 ccggcacacc catcgccctc ctaatccgca acaccgacca gcgcagcaaa gactacggca
     4201 acatcgccac cgccttccgc cccggacacg ccgactatac ctattggcac aaatacggta
     4261 cgcgcgacta ccggggcggc ggcaggagtt ccgcccgcga aaccgcagcc cgcgttgccg
     4321 ccggagccgt tgccaaaaaa tggctgaaag aaaaattcgg cacggaaatc accgcctacg
     4381 ttacccaagt cggcgaaaaa aaaatccggt ttgaaggcag cgaacacatt tcccaaaatc
     4441 ctttttttgc cgccaaccaa agccaaatcg ccgaactgga acactatatg gacggtgtgc
     4501 gcaaatcctt ggattccgtc ggcgcgaaac tgcatatcga agccgccaac gtccccgtcg
     4561 gtttgggcga acccgttttc gaccgcctcg atgccgaaat cgcctacgcg atgatgggca
     4621 tcaacgccgt caaaggcgtg gaaatcggcg caggttttga cagcgtaacg caacgcggca
     4681 gcgaacacgg cgacgaactg accccgcaag gcttcctgtc caaccattcg ggcggcatcc
     4741 tcggcggcat cagcaccggg caagacatct gcgtcaatat cgccatcaaa cccaccagct
     4801 ccatcgccac accgcgccga agcatcgaca ttcacggcaa ccccgtcgaa ctcgccaccc
     4861 gcggcaggca cgatccctgc gtcggcttgc gcgccgcgcc gattgccgaa gctatgctcg
     4921 ccttggtttt aatcgaccac gccctgcgcc atcgtgcgca aaatgcggat gttgccgcgg
     4981 atacgcccga tatttcccgc tcggacaagt aaaaagattt agccaaaaca cgggctttat
     5041 actaaaatac cgaccattgc cgtgccgtct gaacgcacga ttcttagagg aatacaaccg
     5101 aaatgacaca agaaaccgct ttgggcgcgg cactgaaatc cgccgtccaa actatgagca
     5161 aaaagaaaca gaccgaaatg atcgccgacc acatctacgg caaatacgat gtattcaaac
     5221 gcttcaaacc gttggcgctc ggcatcgatc aggatttgat tgccgctttg ccgcaatacg
     5281 attccgcact gattgcacgc gtcctcgcca accactgccg ccgtccgcgc tatctgaaag
     5341 ccttggcgcg cggcggcaaa cgtttcgatt tgaacaaccg tttcaaaggc gaagttaccc
     5401 ccgaagaaca ggcgattgcg caaaaccatc ctttcgtgca gcaggctttg caacagcagt
     5461 ccgcccaagc tgccgccgaa acgccgtctg ttgaagccga agcagccgaa tcttccgcag
     5521 cagaataaat ccccaaacga aatgccgtct gaaaaccgat ttggtttcag acggcatttt
     5581 ttcgtatgcg gcaatcacgg ctcaaatatc gagttgcgcc gtatcgcctt cgcgttccat
     5641 ccaagcgcgg cgggcggcgg cttcgccttt gcccatcagt ttgacgaaga tgtcgcgcgt
     5701 ctcgtcatcc gcgccttcgg ggatttgcac ctgcaacagg cggcgggtgt cggggtgcat
     5761 ggtggtgtct ttgagctggt cggggttcat ctcgcccaag cctttgaaac ggctgatgga
     5821 ataggcggtt tctttaacgc cttctttttg cagccgctct aaaatgccgt caagttcgtt
     5881 ttggtcgagg gcgtagaatt tgcgggcggg tttgcttttg ccttgtgcgt tgacatcgac
     5941 gcggaacagc ggcggctggg cgacgtagat gtgtccgtcg gcgaccagtt tggggaagtg
     6001 gcggtagaac agggtcaaca gcaaaacctg aatatgcgaa ccgtccacgt cggcatcgga
     6061 caagatggct attttgccgt agcgcaggcc gcttaagtcg ggatggtcgt tgatggcgtg
     6121 cggatcgaca ccgatggcga cggaaatgtc gtggatttcg gcgttgccga aaagctggtc
     6181 ggggtggact tcaaagctat tgagcacttt gccgcgcagg ggcaggatgg cttgtgtggc
     6241 tttgtcgcgg gcgagtttgg cggagccgcc ggcggaatcg ccttcgacga ggaagagttc
     6301 gttttcgcgg atgtcttcgc tttcacagtc ggtcagctta ccgggcagga tggcgacgcc
     6361 gctgcctttt ttcttttcga tttttttaac cgaacgtatc cgtgcctgtg cctgacggat
     6421 ggcgagttcg gcgatttttt tgccgaagtc cacgttttgg ttcagccaca attccaaagg
     6481 atcgcccgat acggcggcga caagtttcag cgcgtcgcgg ttggtcagct tgtctttggt
     6541 ctgaccttgg aattgcgggt cgaggacgcg ggcggagagg acgaaggcgg tttttccgaa
     6601 cacgtcgtcg ctttgcactt taacgccgcg cggtaagagg ttgtgcaggt tgatgaaatt
     6661 gttgacggcg ttgaacacgg cttgtttcaa gcctgcttcg tgcgttccgc ccagcgaggt
     6721 cgggataagg ttgacgtagc tttcgttggc gcacgagcct tcttccagcc aagtcaaagc
     6781 aaacgcggca ccttcgccaa tgctgaaatc gccgttgtgg ccgtctgaaa tgtagttttc
     6841 gcaggagaac agcggcacgg cttcctgtgc gtcggcaatc aggtcggtca gatagctttt
     6901 cagaccgtcg gggtaatgcc aggtttgggt gtgcgcttcg tcttcgcctt tgaccggacg
     6961 ggtcagggaa acgcgcacgc ccggcagcag cacggctttg gcgcgcaata gacgctcgag
     7021 ttcgggaatg ctgtaattcg ggctttcaaa atatttgccg tccggccaca cgcgcacttc
     7081 ggtgccgctg tctttgacgg cgcatttgcc cacttgtgcc aacggttcga ccacatcgcc
     7141 gccggcaaac acgatgcggt ggactttgcc gtctcgtttg acactgactt caagtcgggt
     7201 ggaaagcgcg ttggtaacgg atacgcccac accgtgcagg ccgcctgaaa aggcatacgc
     7261 actgccgccg tcttttttgt tgaacttgcc gcccgcgtgc aggcgggtaa acacgagttc
     7321 gactacgggt acgccttctt cagggtgcag gccgacggga atgccgcgcc cgttgtcgcg
     7381 cacggaaagc gaaccgtctt catgaatttg cacgtcgatt tcagtggcga aaccgcccaa
     7441 cgcctcgtcc gccgcgttgt cgatgacttc ttggcagata tgtgtcgggc tgtcggtgcg
     7501 ggtgtacatg ccgggacgtt ctttgaccgg ctccaagcct ttgaggacgg tgatgctgga
     7561 ttcgctgtat tggttgtttt tagccatagg aataatctga aagtaagaaa aacaacgctt
     7621 tcagacggcc tgaaagcgtt gcgttccgtt gttttaccgg ttgccggaag gttggcgggc
     7681 ggcaaagtct tcataacttt ccataccgcg caggaagcgg gaagagagtt ctttcaacgc
     7741 ccccaaataa acgtcgcgtt tgaaatcaat cacttcgtca acgggcgccc aatattgatg
     7801 ccaacgccag ccgtcaaatt cggggtggcg ggtggcgcgc aggttgacat cgcaatcccg
     7861 gccggtcagg cgcaggagat accaaatctg cttctgcccg cgataagagc cgcgccattc
     7921 gcggcgcacc cagttgttcg gcacgtcgta acgcagccag tcgcgcgtcc gcccgacgat
     7981 tttgacgtgt tgcggcagaa gtccgacttc ttcgtaaagt tcgcggtaca tcgcggtttc
     8041 ggggctttcg ccgggcttga tgccgccttg aggaaactgc catgaatgtt cgcgcacgcg
     8101 cttaccccaa aagacttcgt tacgttcgtt aattaggata ataccgacat tggggcgata
     8161 gccttccctg tccaacacgg tgtcgccctc cgttaaattc aatcttggga ttttcccaca
     8221 aatcggacgg ttttgacaaa tcggacggta tggttcaagc atgtcgaaac acggacggat
     8281 tcgggaaaat atcttaaatt tggtttacaa taatacgttt caaattaatt ccggaatcag
     8341 actatgttag atatccaatt gctccgcagc aacaccgccg ccgttgccga acggcttgca
     8401 cggcgcggtt atgactttga taccgcgcgt tttgacgcac tggaagaacg acgcaagtcc
     8461 gttcaggtga aaaccgaaga actacaagcc tcgcgcaaca gcatttccaa acaaatcggc
     8521 gcactgaaag gtcagggcaa acatgaagag gcgcaggcgg ccatggatca agtcgcccaa
     8581 atcaaaaccg atttggaaca ggctgccgcc gatttggatg ccgttcaaaa agaattggac
     8641 gcatggttgt tgagcatacc taacctgccg cacgaaagcg tacctcccgg taaagacgaa
     8701 accgaaaacg tcgaagtccg caaagtcggt acgccgcgcg aatttgattt tgaaatcaaa
     8761 gatcatgtcg atttgggcga gcctttgggc ttggatttcg aaggcggcgc gaaactctcc
     8821 ggcgcacgat ttaccgtgat gcgcggacaa atcgcccgtc tgcaccgcgc cttggcgcaa
     8881 ttcatgctcg atacgcacac gctgcaacac ggctacaccg agcattacac gccttatatc
     8941 gttgacgata cgacgctgca aggcacaggt cagctgccca aatttgcaga agatttgttc
     9001 cacgttaccc gcggcggcga tgaaaccaaa accacgcaat atctgattcc gacagccgaa
     9061 gttaccctga ccaataccgt tgccggcagc attatcccgt ccgaacaact gccgctgaag
     9121 ctgaccgcgc attcgccctg tttccgcagt gaagcgggat catacggcaa agacacgcgc
     9181 ggcctaatcc gccagcacca gttcgacaaa gtagaaatgg tacaaatcgt tcatcccgaa
     9241 aaatcatacg gcgcgctgga agaaatggtc ggccatgccg aaaacatcct gaaggctttg
     9301 gaactgccct accgcgtgat taccctgtgt accggcgaca tgggtttcag cgcggccaaa
     9361 acgtatgact tggaagtttg ggttcccgca caaaatacct accgcgaaat ctcaagctgc
     9421 tccaactgcg aagatttcca agcacgccgc atgaaggcgc gtttcaaaga cgaaaacggc
     9481 aaaaaccgct tggtacatac cttaaacggc tccggtttgg cagtcggtcg cactttggtc
     9541 gcggttttgg aaaaccatca aaacgccgac ggcagcatca atatccccgc cgcactgcaa
     9601 ccgtatatgg gcggtgttac caagttggaa gtgaaataag ccttaaccct gaacataaat
     9661 gccgtctgaa acctgtttca gacggcattt cctttaaact cttaaaacac atcaaccgtc
     9721 ggcacgaacc gcattgccgc aatcgccggt ctgtccgacc tcgcggatat tggacagcgt
     9781 aacttccgaa atattaccca acgcctcttc cgtcaaaaat gcctgatgtc cggtaaacag
     9841 tacgttatga caagacgaca ggcggcggaa cacgtcgtcg gtaatcacat cgttggattt
     9901 gtcttcaaaa aacagctcgc gctcgttctc gtacacatcc atgcccaatg caccgatttt
     9961 ccggcatttc aacgcctcga tggcggcggc gctgtcaatc agcccgcccc ggctggtgtt
    10021 gataatcatc acgccgtctt tcattttgtc gaacgccgct tcgttcagca tatagtggtt
    10081 ttccggcgtg gcgggacaat gcagcgtgat gatgtccgat cgggcataca gctcgtccaa
    10141 atccacatac ctgccgccga gtttttccac ttcggggttg caaaacggat cgtaagccag
    10201 caggttcata ccgaaaccct ttaaaatccg catcgttgcg ataccgattt tccccgtgcc
    10261 gataacgccc gccgttttgc cgtacatatt gaagccggtc agaccttcca gcgaaaaatt
    10321 cgcatcgcgg gtacgctggt aggctttgtg gatacggcgg ttcaaagtca gcatcagacc
    10381 gaccgtatgt tccgcaaccg attcgggcga ataggcaggc acgcgcacga ctttcaagcc
    10441 caactcttca gccgccttca aatccacatt attgaagccg gcacaacgca acgccacagt
    10501 tttcacgcca atttgcgcca atttttccaa cacgggccgg ctggcgtcgt cgtttacaaa
    10561 aatacaggcc gcttccgcgc cttccgccat tttcgccgtt ttcgcatcca gcatgaaatc
    10621 aaaaaactcc agctcgaggc cgaaatgccg gttggcgcgg gtaaaatgtt cgcggtcata
    10681 gcttttcgta ccgtaaatcg caatcttcat caatatgtcc agttgtcgtc tatggttgag
    10741 aaacggcata atacaccgaa ttcaaacaaa tcagtagaat atggcggatt aaaattgatt
    10801 tcatgcacgg catttccatt tcaaaacaca aaactcaatc gcccatcgcc gccagaagct
    10861 ccgcctgatg ttcggcaatc agggcatcgg tgatttctgc caaatcgccg tccatcacaa
    10921 aatccagctt gtgcagggtg aggttgatgc ggtggtctgt taccctgcct tggggatagt
    10981 tgtaggtgcg gatgcgttcg ctgcggtcgc cgctgccgat gagggatttg cgttcggcgg
    11041 cttctttggc ttggacttcg cgtttttgcg cgtcgttcag gcgggcggcg aggactttca
    11101 tcgcctgcgc tttgttggcg tgttggctgc ggccgtcttg gcattcgacc accatgcctg
    11161 tcggcaagtg ggtaatgcgg acggcggagt cggttttgtt aatgtgctga ccgcccgcgc
    11221 cggatgcgcg gaaggtatcg gtgcgcaggt cggcggggtt cagttcgatg tcttcgagct
    11281 cgtccgcttc gggcatgacg gcaacggtac aggcggaggt gtggatgcgg ccttggcttt
    11341 cggtggcggg gacgcgctgc acgcggtgtc cgcccgattc aaatttcaga cggctgtacg
    11401 cgccgagtcc gacaatacgg gcgatgactt ctttatagcc gcccaattcg ctttcgttgg
    11461 cggacacgat ttcaacctgc cagcggttgc gttcggcgta gcggctgtac atacgcagca
    11521 aatcgccggc aaacagcgcg gcttcgccgc cgcccgtgcc ggcgcgtatt tcgataaaga
    11581 tgtttttgtc gtcgtcggca tctttgggca gcagcagttt ttgcaattcg gtatcgagtt
    11641 cgtcgatttt ggctttcgcc gcttcgattt cttcggcggc aaagtctttc atttcggggt
    11701 cggacaacat ttcttcggca tccgccaagt cgctttgggc aagccgatag ttttggaata
    11761 cttcgacgac gggggtcagt tcggcgtgtt cgcgcgtgag cttgcggtag ttgtccatat
    11821 cggatgtggc ttcgggttgc cctaaaaggt gggtaacttc ttccaaccgg tcgccgagtt
    11881 gttgcagttt ttctaagata gacggcttca taattcttcc ataacaaacg ccgcctgaat
    11941 ttcagacggc atcaaaacgc ggtcattata atatattttc tgaatattca aaaagataat
    12001 ctagggacgg agcagactgt cggcattgcg ttccgttatc gcggcgattt ccgccgcgcc
    12061 ggtaccgcgt atttccgcag caatttcggc aatccgaaga atgttggcag gcgtattgac
    12121 ggtgttttcc aacatgaacg ggctatccgt ttccaatacg aaatccccgt cgttcaaggc
    12181 tttaagcgta tcgcgcactt tacgcgcgtt cggattgagc agcagcgaac cgatgccgat
    12241 tttgaacccc agttttgtca acacgcgcgc ttcttccgcg ctgcctgaga aagcgtgaac
    12301 gatgccgcct tgagtaaagc ctgtctgttt gacggcggcg gcgatgtcgg cggtggcttt
    12361 gagattatgg ataatcacgc ggcggcgcag ggtttgcgcg atggcaagct ggcggctaaa
    12421 aacttgaatt tgccgttcac gctgcggcgg cgtttgggtt ttgtcgtaaa aatccaagcc
    12481 gatttcgccg acccacgctc gggggtaacg caccaatacg gcttccaaac cggaaaaatc
    12541 ctgttccgca atgccgtctg aaaaccaagg atggatgccc agcgcaatac ggatttgacc
    12601 gtgttcggac ggcatttccg acagttccgc cacgtcctgc caatcctgcg gacaggtagc
    12661 gggaacgata aaccgccaca cgcccgcctg tcgcgcttcg gcaagcacct gcgccagatt
    12721 gccgcgcaaa acggaatcgg caagatggca atgtgtgtcg gtcagcctca tcttcacacc
    12781 agccccaacc gctgcaactc gtgtttcacc ccgtccgcgc cccatccttc ggaaacggca
    12841 agcgtcatca atgcggttgc ggtttccaaa ttgcatttgc cgccgttgac cacgcccgcc
    12901 cgacgcagcg cgtcgccctg tgcgtaaacg gcggcagcgc aaccctgcgg cacttggctg
    12961 atgttaagca acaatttgcc ttgctgcgta aagtcttgca cggcgcggat gaagccttcg
    13021 ccggcggagg tattgccgtg tccgtagctt tgcaggataa gggcttgggc gggaagctgt
    13081 ccgagcccgt cggcaagttc ttggacggca aagccgggga taagcgtgcg gacggcgatt
    13141 tttgcctgcg ggtcgggata acggatttcg aggccgtctg aaacatctgc cgcgtcctga
    13201 ttcgggatgc ggacattgtg ccagccccgg gtttcgtccc actcggcaag tgcgccgaaa
    13261 tgggcattgc cgaaaccggc ggcggtttcc gtgctgattt tgctgctgcc gacggcggga
    13321 tacagtttgc cgtcaaaggc aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg
    13381 gcagtcacca gattgcgcgg agcgtcgctg ccctctgcgt tgtacggcca ttgcgaaccg
    13441 gtcaggacga tcggtttgtc caaaccttgc agggcgaggg cgaacatatt ggcggtatag
    13501 gcgagtgtgt ccgtgccgtg cagcaccaaa acaccgtcgt actgcggcag cgcggcggca
    13561 atgatgtcca gccaagcgca ccagttttgc agttggacgg cggaagaatc aatcagcggc
    13621 tcacaaacgt gccaatcgaa atccatgccg tctgaaaacg gcgcgagtgc ctgcccgacc
    13681 agcgcggtat cggggcgcaa gcccgcactg ctttgcgtca tgccgatggt gccgccggta
    13741 taaaggacaa aaattttctg tttcatggct cattctgaaa atgcaaagac cgcctgaaac
    13801 gcgaaatgcg tccggacggt cgaaaatccg atttactgct gcttctgtgc cgcttttttc
    13861 gctgcctttt ccgccttgcg tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga
    13921 taatgtcggc gtttgcgcca ccagaaccac gccagcgccg ccgccagcac gcccaatgcg
    13981 atgaagatgc ccgattgcag gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg
    14041 ccgtactcgc ccaagtaaat ccaaacgggc acggaaatca gcgcggccag cccgtccata
    14101 atcagaaagc gcagatacga tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc
    14161 cgcaaacccg gcaggaaacg ggcgacaaac agaacccagt tgccgtattt gtcgaatttt
    14221 tcctgaacct gcgcgtaacg tttcggcgtc atgatgcgcg caatcggttt gaacttgagg
    14281 attttctgcc cccagatgcg tccggcggca aacatcacgc cgtcgcccgc caacacgccg
    14341 agcataccga ccgcaaacat aatatgcgga ttggtataac ccatacccga aatcacgccg
    14401 cccgttacca aggtcaaatc ttcgggaatc ggcacgccga aaccgcagat gaccaaaacg
    14461 aaaaacacgg ccgcatagcc gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg
    14521 gatattccat tgtcggagat aaaaagtcag aacaagccga aacattttct gcgcgaaacg
    14581 gcattctatc aaagattatg ccgtctgaaa gcggcagggg gggcagattc gcgccccgcc
    14641 ggacgttcag acggcattta aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt
    14701 ttgcctaaat gccgccgatg gcggcggcga tacgctccgc cccgtcgcgc gcccaatcgg
    14761 cctgacgcgc ctcgaccatc acgcgcacga cgggttcggt tcccgaagcg cgcaacacga
    14821 cacgcccttt gccttcgagt tctttttcca cttcggccaa cacgtctttc gaagcctcct
    14881 gccatttctg acccttttgg atgcgcacgt taatcatcgt ttgcggatac ggctgccagt
    14941 cggcgcaaac ggtggcgagg tcttggttca ggatttgcag cgccgccaag acttgcagcg
    15001 cggaaataat gccgtcgccg gtgttatgtt tgtccatgca caaaatatgg ccgctggctt
    15061 cgccgccgat gagccagccg cgttggttca gctgttccaa cacatagcgg tcgccgactt
    15121 tggcgcggca gaaatcgacg ccctgctctt tcagggcgat ttccatcgcc atattggtca
    15181 tgaccgttcc gaccacgccg ccgatgttga taccttctcg ggcgcgggct ttggcgatga
    15241 cgtaaatcag gctgtcgccg tcgtaaacct gcctgttttt atcgaccatc atcaggcggt
    15301 cgccatcgcc gtccaaggca ataccgtaat cggcttcgtt ttgcaacacg gcggcttgga
    15361 gggttttggt atgggtcgcg ccgcattttt cgttgatgtt gtagccgttg ggttcgttgc
    15421 cgatgctgac gacctgtgcg ccgagttcgt ggaacacttt gggtgcaacg ccgtatcctg
    15481 caccgttggc ggtatcgata accaacttca aaccccgaag gtcggaatgg ccggggaagg
    15541 tggatttgca aaactcgata tagcggtcgt ccgcaccgct gatgcggcgg gcgcgtccga
    15601 gacgtgcgga cggttgggtt ttcatttctt catcgatttt ggcttcgatt tccaactcga
    15661 cttcatcgga aagtttcacg ccgccttcgg caaagaattt gatgccgttg tcggaatagg
    15721 tgttgtgcga cgcggaaatc atcacgccgg cggacaggcg caatgcgcgg gtcagataag
    15781 ccacaccggg cgtgggcaac ggcccggtct gtaccacatt cacacccgcc gccgtaaagc
    15841 ccgctaccag cgcggcttcc agcatatagc ctgaaatgcg cgtgtctttg ccgatgagga
    15901 cggtcggttt ctgatcggta tcgtgctgca ccagcacctg ccccgccgca tagccgagtt
    15961 tcaatacaaa atcgggcgta atcggaaact gaccgacttc gccgcgcacg ccgtccgtgc
    16021 cgaaatattt ttttgccatc atttgctccg tgaatgtgaa ccgttgtccg agattataca
    16081 gtcagtttgc gccctgctgc ctgcaccgtt gatgccgtct gaagccgtcc cgcccttttc
    16141 agacggcatt cggcgcaaaa accgccgttt accggttcac gcccaacgct tcccacacct
    16201 tcaacgcgtc cgccgtcgcc ttcacatcgt gcacgcgcac gatttgcgct ccgcgtgcca
    16261 cggaagccaa agccgctgcc acgctgccgt gtacgcgcgc cgccgcgtcc gcctcaccgg
    16321 tcagcttacc tatcatgctt ttgcgcgaca caccgatcag cagcggcaaa ccggtttccg
    16381 ccatcaattc gggcaaatgc cgcatcagtg cgatattgtg ttgcaggttc ttgccgaagc
    16441 cgaaacccgg gtcgagcgta atccgttgcg gcgcgatgcc tgccgcgaca caggtttctg
    16501 accgtgtttt cagataacgt gccacttcgc cgaccacatc ctgatatttc ggattgtcct
    16561 gcatggtttc gggcaagccc cgcatatgca tcaggcaaat gcccgtgtcc gcctggcgcg
    16621 ccagcaattc gaccgcacct tcgtcggtca acgccgccac atcattgata atatcgatgc
    16681 cgccgagtgc caacgccttt tccataacca ccgtgcggcg cgtgtccaaa ctgacgggaa
    16741 cgccccaccc cgccgcttcc gccaatacag gctcaacccg cgcccattct tcttcgggcg
    16801 gaacgaaatc cgcacccgac cgcgtcgatt cgccgccgat gtcgagaatg tccgcaccct
    16861 ctttcaaaag ccgttcggca tgcgccaaag ctgtttgggc gttttgcgaa tacgcgccgc
    16921 cgtcggaaaa agaatcgggc gtgagattca cgatgcccat gattttcggt ttgtccaaac
    16981 cgatttcaaa ccgtcctgcc cgccaaacgt gtcgtgccat ctgaactcct cccgaaataa
    17041 aaaacagatt atatgccgtc tgaaaccgtc ttgtgcgctt cggacggcac cgctattcgg
    17101 gcggcagacg gcatgttgtc ccaatgtctg cgccgccttt gaatctgccg gcatgcctgc
    17161 tatccgcccg ccttttcaaa acaggttccg acgattccgc acgcgcctgc cgcctttgcc
    17221 aagccgtaca ggatttcctg cggcatatcg cggctccata atcccgtaat attcgcaatc
    17281 acgggcagat ggctgatttg gcggactttc acgatggatt cgacatccaa acggtaggga
    17341 tggcctttgg tatggcccaa cacgcccgac tcgcccagaa tcaggtggcg gttgccgcgc
    17401 gagacgacat attctgcggc attcaaccaa tcttcggcag aatggtgctt gtctttacac
    17461 agaaccaagg gaatgttcag gcttccggct tcattcaata cggcaagatc ggacatcagc
    17521 ccgccgccca aatacaggat gtccgctccc gcattcaaag ccgcttcgac atggcggacg
    17581 ttgcgaacgc gcaccaatac gggtttccct gcgccatgcg ccgatgcggc ctgttccgcc
    17641 aaccgtctgc accgtccccg cccttcatcc gcacttaaag tgtcgtataa gtttgccgaa
    17701 gtgaaaaacg gatccagaaa cactgcatcc gcattgctcc atactgacgg ttctgcagcg
    17761 atacggacgg tttccccgcc gccgaaagcc acgcctttgg cggcaacgcg gctgtcttcc
    17821 gcccgatttt cccgactgac ggttttccat gtgtccaaaa tgcggacggc tttctcgacc
    17881 tccggcagcg tctgtacctc cctgacgctc aaaaccctat cgtcgccgat tgcgccgatg
    17941 acggtacgct cgtcgccgtg agaaatgtgt tctcgcagac ctctgctgcg gatgaaggcg
    18001 acaacgccgg caatgtccgc ttcggcggca cgcctgctca tgacaataat catatttcct
    18061 cctgacacaa gagacggccc gcccaaaata ggatttttgc aagccgtgtt atactgtggc
    18121 gtgttttaca gattgttcgg gctatggatt tattatcggt tttccacaaa taccgtctga
    18181 aatatgcggt ggcggtgctg acgatgctgc ttttggcggc agtcgggctg cacgcttccg
    18241 tatatcgcac cttcacgccc gaaaacatcc gcagccgcct ccaacaaagc attgcccata
    18301 cccaccggaa aatctcgttt gatgcggata tacggcgcag gcttctgccc cgcccgaccg
    18361 tcatcctgaa aaacctgacc attaccgaac ccgacggcgg ccgggtcgcc gtttccgtca
    18421 aagaaaccaa aatcggattg agctggaaaa acctgtggtc ggatcggata caggttgaaa
    18481 aatgggtggt ttcgggtgcg gatcttgccc tgacgcgcga cagaaacggc gcttggaaca
    18541 tccaagacct gttcgacggc gcgaaacact ccgcctcagt caaccgcatt atcgtcgaaa
    18601 acagcaccgt ccgcctcaat ttcctgcagc aacagcttat cctgaaggaa atcagcctca
    18661 acctgcaatc ccccgattcg tcggggcagc agtttgaaag ttcgggcata ctggtttgga
    18721 gaaagctgtc cgtcccgtgg aaaagcaggg ggctgttcct ttcagacggc atcggcacgc
    18781 ccgaaatctc accgttccat tttgaagctt ccacttcgct ggacggacac ggcatcacca
    18841 tttccaccac cggcagccct tctgtccgct tcaacgccgg cggagcggat gccgccggcc
    18901 tcggcctgcg tgcagacact tccttccgca acctccacct gaccgcgcaa atccccgcac
    18961 tggcactcaa aaacaacagc atcaaaaccg gcacggtcaa cggcacgttt accgccggcg
    19021 gcgaatatgc ccgatgggac ggttcgttca aactcgacaa agccaacctg cactccggca
    19081 tcgccaacat cggcaacgcc gaaatctccg gcagcttcaa aacaccgcgc cttcaaacca
    19141 atttctccct cggctcgccg ttggtttgga gtcgggacaa cgggctggac gccccgcgcc
    19201 tgcacatatc gacccttcag gataccgtcg accgcctgcc gcaaccccgt ttcatcagcc
    19261 ggctcgacgg ttcgctgtcc ataccgaatc tgcaaaattg gaatgccgaa ttaaacggca
    19321 cattcgaccg ccaacccgtt gccgcaaaat tcaaatatac gcgggaaggc gcaccgcacc
    19381 tggaagccgc cgccgcgctg caaaaattaa acctcgcccc ctatcttgac gaatttcggc
    19441 aacaaaacgg caaaatattc cccgacatcc tcggcaggct gtccggcaac gtcgaggcac
    19501 acctcaaaat cggcagcatc caactccccg gcttgcaact ggacgatatg gaaacctacc
    19561 tccacgccga caaagaccat atcgcgctca gccgtttcaa gtcagggctt tacggcggcc
    19621 ataccgaagg cggcatcagc atcgccaaca cccgtcccgc cacttaccgc ctgcaacaga
    19681 atgcaagcaa catccaaatc caaccgctgc tgcaagacct gttcggcttc cacagcttca
    19741 gcggcaacgg cgatgcggtc atcgacctga ccgcaagcgg cgaaaaccgc aaacagctta
    19801 tccgctcgct gcaaggcagc ctgtcgctga atatttccaa cggcgcgtgg cacggcatcg
    19861 atatggacag cattttaaaa aacggccttt ccggaaaaat ctcgggcagc acacccttct
    19921 accgattcac gctcaacagc gaaatttcag acggcatcag ccgccacatc gataccgaac
    19981 tcttctccga cagcctctat gttaccagca acggctatac caatctggat acgcaggaat
    20041 tgtctgaaga tgtccttatc cgcaacgccg tccatccgaa aaacaaaccg attcccctga
    20101 aaatcaccgg tacggtggac aagccgtcca ttaccgtcga ttacggcagg ctgaccggcg
    20161 gcatcaattc gcgcaaagag aaacagaaaa tcctcgaaga caccctgctg gaacaatggc
    20221 agtggctcaa acctaaagaa ccgtaaatac cctgcgtaca aaaatgccgt ctgaaacaca
    20281 ttaatcttca gacggcatga ccgtaaaacc tacaacccca atttccccca aatcccatca
    20341 attttaaccg taaccgcagg gtcttttttg attacccgtc cccattcgcg gtcggtttct
    20401 cccggccatt tgctggtcgc atccaaaccc attttgccgc cgagtccgct gacggggctg
    20461 gcgaagtcga ggtagtcgat gggcgtgttt tccaccaaaa cggtgtcgcg gacggggtcc
    20521 atgcgcgtgg ttaccgccca gatgacttct ttccagtcgc gcacgtttac atcgtcatcc
    20581 accacgatga tgaatttggt gtacataaac tggcgcagga acgaccagca gcccgtcatc
    20641 acgcgcttgg cgtgtccggc gtactgtttt ttcatgctga ccaccgccat gcggtaggaa
    20701 cagccttcgg gcggcaggta gaaatcggtg atttcggaga actgcttttg caaaagcggt
    20761 acgaacactt cgttcaacgc cacgcccaaa acggcaggtt cgtcgggcgg tttgcctgtg
    20821 taggtagagt ggtaaatcgg gttttcgcgc atggtgatgc gttcgaccgt aaacacaggg
    20881 aaatggccct gctcgttgta atagcccgtg tggtcgccgt atgggccttc caacgcggtt
    20941 tcgtttggat gaatcacgcc ttccaataca atttcagcac gggcaggcac ttgcaaatcg
    21001 ctgccgatac atttcaccag ctccgtccgc gaaccgcgca gcagtccggc aaactggtat
    21061 tcgctcaaag tatcgggaac gggcgttacc gcgcccaaaa tggtggaggg gtcgcaaccg
    21121 agtacgacgg cgacgggata cggcgtatcg ggattgagtt tgcggaattc ctgataatcc
    21181 aacgcgccgc cgcgatgcga cagccagcgc ataaccagct tgtttttgcc gatgagttgt
    21241 tggcggtaaa tgccgagatt ttggcgtttt ttgtgcggcc cgcgcgtgac ggtcaagccc
    21301 cacgttacca gcggcgcaac gtcttccggc cagcaatgtt gaatcggaag ctgatacaaa
    21361 tcaacgtctt ctccttccca cacgatttcc tgacacggcg cgtttttcac cacgttcggc
    21421 gccatgctcc aaatatcttt caacagcggc agtttggaaa acgcgtcttt gatgcctttg
    21481 ggcggttcgg gttctttcaa atacgccagc gtctgcccga tttcgcgcag cttggacacg
    21541 ctgtccgcgc ccatgcccat cgccacacgt tcgggggtgc cgaacaggtt cgccaacacg
    21601 ggataatcat agcgcgtacc gtcgggctta atcgggtttt caaacaacaa cgccggccct
    21661 tcggcgcgca acacgcggtc ggcaatttcg gtcatttcca aatgcgggga aacggggtgg
    21721 gcgacgcgct tgagcttgcc ctcctgctcg agcatagcga tgaagtcgcg caggtctttg
    21781 tatttcatgt ttatcctttt tgtcctttta tcctgaacaa tccgattcgg ataccgcccc
    21841 tatccttgcc tgcgcttcgg catatcccat gccgtgataa aagtcgcgta ccggcggatg
    21901 tccgccgctt tgatgaagtt gcagcaaagg gcgttggttg tcggactggg taacgacatt
    21961 gcagtgcaga ccgaaggtgt cggtttcata agggggcagc cgattgcaaa tcataccgaa
    22021 ataaacggcg ttttcagggt tgtcgtaaaa gcggctttga tagtcgttaa aactcttttc
    22081 gctgacggac acccacacgc cgtattccag cgtttcctga tgcccgataa tcggaatagg
    22141 cagcgccgcg cggataaagc ggtcggtttg gccggaatag cggatgatgc agaaatcaga
    22201 atcgcattcc gcttgataag caatgcgttc ttcttcactg agttgattat agggataggg
    22261 ggcggtaaag ccggttgcgg gcatatcttc atgtttttcg ccgcaggaag tgcaagtgtg
    22321 cataaggttt cagactttca acacaagttt gcggtaaagc cattcgccgg caaacagcat
    22381 ccccatcaat acataggcaa tcacgcctgt ataaaccgcc caccaatcat atcgccccaa
    22441 ccgggccaac aaagcggcaa gcgtcccgtt ggcaataaag aatacgcacc aaacctgcgt
    22501 tacccggcgg gtatagcgca cggctttttc aggcaggtcg ggctgttgca gccgcgcaag
    22561 tttttcaatc accgtctgcc cggcaaacag gctgctgccg aacaccgcca acatcatcaa
    22621 attaacgagg acaggatacc aatacattga atcacgccgc ccgaacacca atactgcggc
    22681 aaaaaacagt gtaatatagt ggattaaatt taaatcagga caaggcggcg aagccgcaga
    22741 cagtacaaat agtacggcaa ggcgaggcaa cgccgtaccg gtttaaattt aatccactat
    22801 accacaaagc gggataggca atgcttaata cggtcaaaaa aatatgtccg aaaaaaccgg
    22861 gtttcatttt gaatccgcac aaacgttttc agacggcatc cgataaaaac atgccgtctg
    22921 aaaaataatt agaaataccc gattagccgg cctgaatctt caataccgcc tgtaccacgt
    22981 cgttgacggt gcggacattg cggaaatctt cagcctgcag cttgcgggct gccacaacca
    23041 ttcacgattg cgatataccc ggcggcattc ccgactgccg ttcagcagaa aacgcaccca
    23101 ttccaaaccg ctccaatatg cctcgggcag cataccggct tcagacggca tatcgtccga
    23161 agcagacaaa gtcaggctgt aacgcgtccc tttggtcaga accatcgcca aagcataagc
    23221 aaacggcgcg cgcgttgccg atacggcata tccttccggc agcggatcgt ccgccgccaa
    23281 aaccaaaacc gacctgcatc cctcttccaa cagggacgcc gcttccgcca atgccgtttc
    23341 cacaccgtcg gcacacacgg ccaatgccgt ctgctcgctc atatcccgcc tcaatatcga
    23401 ccattgcccc gccgtcgcat tgtgcaccga caaaccgaac gaagtcggcg acacggtatg
    23461 cgatttcaac agttccaacc acaaatcgaa actacgcgcc atttccccgt cgtgcgaggc
    23521 ataaactacc ggactgccgg gatgggcgga ggcaatgtcc caagccgcgt cgcataccaa
    23581 acgcgccgcc ttactcaaac ggcggcgccg catagcgggc aggaacggca attcccgcct
    23641 gacatcgggc aaaccgtcgg caaaatccgg acattccgcc cactgcctct actgggccat
    23701 atcgcgcatt ttgccgcccg aaacccgcca gtcggcgatg tcgaagtgga accggcaaat
    23761 aatcggcggc atagtttctt tcaaaaattt acactgtgcc gcattctaac caaagcccat
    23821 ccccctgaca atgccgaaat tcaaacgcat ccacgccatt ttttccgaca acgccgcccc
    23881 tcggcaaacc gcccgaatta gcctgaattt acatttatca ttataatgcc cgattaccga
    23941 caccgcctcc ctgctgccgc acagcgggcg tatggttctg atagaccgca ttacccgata
    24001 cggcgatgat tttgtcgaag caggggcgca ggtaaaaccc gaccacatcc tgctggccgg
    24061 cggcatgctg ccctatacgg catttatcga actgatggcg taggctgtcg gcgcatatgc
    24121 cggcctccaa gcccgaaaaa aggcgcaacc ggtccggctc ggttttcctg cccggcacgc
    24181 gcaaacttga aatttttacc caatccgtcc ctgtcggaac acatttgccg gcaacggcgc
    24241 atatgtctat ccaagatgcc ggggtatggg cgtgtccgac tgcgaactgc gttggacgga
    24301 cgcgccggaa accttgtctg aaacactcct ttcagacggc attttggcgc gcgcctcact
    24361 caacgtgtac agccccgaac accctgtcga aacaacttaa tgccgtctga acaggcacgc
    24421 acatacggag aacaaccatg actgaaaccg tcctgattac cggctcaaac agaggcatag
    24481 gcaaagccgc cgcactcggt tttggcggac gacggctttg atatcgccgt ccactgccgc
    24541 agccgccgcg acgaagccga agccgtggcg gaagaaatcc gcgctttggg cagaaatgcg
    24601 cgcgtgttgc agtttgacgt gtccgaccgc gaagcctgcc gcgagattct gaccgccgac
    24661 atcgaagcaa acggcgcgta ttacggcgtg gtgttgaacg ccggcctgac gcgcgacaat
    24721 gccttccccg cgttttcaga cgacgattgg gatgtggtgc cgcggactaa tttggacggt
    24781 ttttacaatg tattgcatcc gctggttatg ccgatgatac gccgccgcaa agccggacgg
    24841 attgtgtgta tggcatcggt gtccggcctg acgggcaacc ggggacaggt caattacagc
    24901 gcgtcaaaag cgggcattat cggcgcggca aaagccttgg cggtcgaact ggcgaaacgc
    24961 aaaatcaccg tcaactgcgt ggcgccgggt ttgatcgata cggagattgt cgatgaaaac
    25021 gtacccgtcg gagaaatttt gaaagccgtc cccgccgcgc gtatgggact gccggaagaa
    25081 gtggcgcacg cagtgcactt cctgatggat gaaaaagcgg cgtatatcac gcgccaggtg
    25141 attgcggtga acggaggttt gtgttgaata ccagaagggt cgcagtaacg ggcataggcg
    25201 gcattaccgc cttcggccgg gattggcaaa gcatacaggc agcattcaaa gccgaaaaaa
    25261 acgccgtcaa atatatggat tggcgcgaac gtttccccga attggaagcg caactgggtg
    25321 cgccgattga ggattacgcg ccgccgaaac attggacgcg caagcggctc agaagtatgg
    25381 ggcgcgtgtc gtacctgtgc gtcgatgcgg cggagcaggc tttgatggat gcaggtttgc
    25441 tcggggacga aagcattacc gacggacgga tgggcgttgc ctgcggctct tccggcggcg
    25501 gcaccaaaga catcggcgat gtgggcgaat tgttgctgac cggcacgtcg cgcaacttcg
    25561 gcgccaatac ctatgtgcgt atgatgccgc acaccgccga cgccaatatc ggcatctttt
    25621 tcggattaat aaggacgcat catcccgaca tcgagtgcgt gttcttcggg cagccaaggc
    25681 ataggttatg cttacgaggc catcaaatac ggtctgaccg atataatgct ggcgggcggc
    25741 ggcgaagaat tttgcccgtc cgaagtgtat gttttcgact cgctctatgc cgccagccgc
    25801 cgcaacggcg aaccggaaga gaccccgcgt ccatacgacg cgaaccgcga cgggctggtc
    25861 atcggagaaa aggcgcgggg attttcgtat tggaagaatt ggaacacgcc aaacggcgcg
    25921 gtgcgaaaat ttacgccgaa ctcgtcggct acggcgccaa cagcgacggc atcaatatcg
    25981 gcgaaggcgc agccgtattc atcatgacgc gcgatgcgga tttttccggc ggtatgcagc
    26041 ttctgggtta cggcgcaagc agcgatgcct accatatttc cacgccccgc cccgacgcaa
    26101 aggcgcaatc ctcgcctttc agacggcatt gcagcacgcc ggccttgcgc ccgaagacat
    26161 cggccggatt aatctgcacg gcaccgggac gcaccacaac gacagtatgg aaagccgcgc
    26221 cgttgcagcg gttttcggca acaatacgcc ctgcacgtcc gccaagccgc aaaccggaca
    26281 cacgctgggc gcggcgggcg caatcgaagc cgcgttcgcg tggggcattg ccgaccggca
    26341 aagcaatccc gaagggaaac ttccgccccg gctttgggac gggcagaacg accccgacct
    26401 gcccgccatt aacctgaccg gcgacggctg ggaaaccgaa aaacgcattg ccgccagctc
    26461 gtcgtttgcc ttcgggggaa gcaactgcgt cttaatcatc ggatgaaata agtttgtcaa
    26521 tcccaccgct atgctataca atacgcgcct actcttgacg ggtctgtagc tcaggggtta
    26581 gagcagggga ctcataatcc cttggtcgtg ggttcgaacc ccaccggacc caccaattcc
    26641 caagcccgga cgtatgtttg ggcttttttg ccgccctgtg aaaccaaaat gctttgagaa
    26701 accttgataa tgaaaaaagt cagcgtattg attgttgcca aaaacgaagc aaaccacatt
    26761 cgggaatgta ttgaaagttg ccgtttcgat aaagaagtta tcgttatcga cgaccatagc
    26821 gccgacaaca ctgccgaaat tgccgagggt ttgggtgcaa aagtcttcag acggcatttg
    26881 aacggggatt tcggagcgca aaaaacattt gccatcgaac aggcaggcgg agaatgggtt
    26941 ttcctgattg atgcagacga acgctgcacg ccggaactat ctgatgaaat ctcaaaaatt
    27001 gtccgaaccg gcgattatgc cgcctatttt gtcgaacgcc gcaacctttt ccccaaccat
    27061 cccgccacac acggcgcgat gcgtcccgac agcgtatgcc gtctgatgcc gaaaaaaggc
    27121 ggttcggtgc aaggcaaagt acacgaaacc gtacagaccc cctaccccga acgccgtctg
    27181 aaacatttta tgtaccatta cacgtacgac aactgggaac aatatttcaa caagttcaac
    27241 aaatatactt ccatctcggc cgagaaatac cgggagcagg gaaagcccgt gagtttcgtt
    27301 agggacatta tcctccgtcc gatttggggt tttttcaaaa tttatatcct gaacaaaggg
    27361 tttcttgatg gaaaaatggg ttggattatg tccgtcaacc acagctatta cacgatgatt
    27421 aaatatgtca aactatatta tctgtacaaa tccggcggaa aattttaaat ggaaaaagaa
    27481 ttcaggatat taaatatcgt atcggccaag atttggggtg ggggcgaaca atatgtctat
    27541 gatgtttcaa aagcattggg acttcggggc tgcaccatgt ttaccgccgt caataaaaat
    27601 gatgaattga tgcgcagacg cttttccgaa gtttcttccg ttttcacaac gcgccttcac
    27661 acgttcaacg ggctgttttc gctctacgcg cttacccgct ttatccggga aaaccacatt
    27721 tcccacctga tgatacacac cggcaaaatt gccgccttat ccgtactttt gaaaaaactg
    27781 accggggtgc gcctgatatt tgtcaaacat aatgtcgtcg ccaacaaaac cgatttttat
    27841 caccgcctga tacagaaaaa caccgaccgc tttatttgcg tttcccgtct ggtttacgat
    27901 gtgcaaaccg ccgacaatcc ctttaaagaa aaataccgga ttgttcataa cggtatcgat
    27961 accggccggt tccccccgtc tcaagaaaaa cccgacagcc gtttttttac cgtcgcctac
    28021 gccggcagga tcagtccgga aaaaggattg gaaaacctga ttgaagcctg tgtgatactg
    28081 catcggaaat atcctcaaat caggctcaaa ttggcagggc acggacatcc ggattatatg
    28141 tgccgcctga agcggggcgt atctgcttcg ggagcagaac catttgtttc ttttgaaggg
    28201 tttaccgaaa aacttgcttc gttttaccgc caaagcgatg tcgtggtttt gcccagcctc
    28261 gtcccggagg cattcggttt gtcattatgc gaggcgatgt actgccgaac ggcggtgatt
    28321 tccaatactt tgggtgcgca aaaggaaatt atcgaacatc atcaatcggg gattctgctg
    28381 gacaggctta cgcctgaatc tttggcggac gaaatcgaac gcctcgtctt aaaccccgaa
    28441 gcaaaaaacg cactggcaac ggcaggacat caatgcgtcg ccgcccgttt caccatcaac
    28501 cataccgccg acaaattatt ggatgcaata taagccgctt tcagacggcc tatgccgtct
    28561 gaaagccttt gatgcaacaa accgccaaat tatattcgtt cattggaaaa aaacaccccg
    28621 aattcatcct tcaaaataag aaaatcccaa tatcccccga tatcgcgcag cctattggca
    28681 aagttttgca gcgtcttccc cggcttgtgc cgccgcgtca agtgctttgt tacaatgcac
    28741 taccttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
    28801 gtttcgtact tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
    28861 atacggcggg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
    28921 tggtcgccct gcccgtccag cttgccgaat tttatatcgg gcgcacgggc ggtaaaaatg
    28981 ccgtcgattc cttcagggtt ctgcgtccgg gtacgcaatg gctttgggtc gggcgcatgg
    29041 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
    29101 attatgtcgt ccacagtttt acaggggcaa tccatgcggg agcggacttt gaagccctgt
    29161 tcggcacaac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
    29221 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
    29281 tgatgccggg gctgtttatc ctctttattg cgctggctgt ccgttcgctg acgctgccgg
    29341 atgcaatgga gggcgtgtct ttcctgctca aacccaattg gtcgtacttt aaagccgata
    29401 cgatgattac ggctttgggc caggcgtttt ttgccctgag catcggcgtt tccgccatga
    29461 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgttccggc catacgatta
    29521 tgtggatgaa cctcttggtt tccctgcttg ccggcctggt gattttcccg gcggtattcg
    29581 ccttcggttt tgaaccgaac caggggccgg gattgatttt tatcgtattg cccgcagtgt
    29641 tcatgaaaat gccgttcggt acggttttat ttgcagtatt catgcttctg gtcgttttcg
    29701 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
    29761 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttc attgtcggca
    29821 tcccgtccgc gctgtctttc ggcgcatggg gtgagtttaa ggttttcggc aaaaccattt
    29881 ttgatttgtg ggactatgtt atttccgccg tcattatgcc gattggtgct ttgagtgttt
    29941 ccatctttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
    30001 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
    30061 ttgccattat tattgttttc gtcaattctt taggcatcct ttaaacatac cgcccgacgg
    30121 caaaatgccg tctgaaagcc tttcagacgg cattttgctt cagattcaac ctatttcgtt
    30181 caaagtatag tggattaaat ttaaatcagg acaaggcgac gaagtcgcag acagtacaga
    30241 tagtacggca aggcgaggca acgccgtacc ggtttttgtt aatccactat attgtccggc
    30301 atggccagcc taactttaag ccgcctgctt tcggcaaccg accgtatcag cggcaaatcc
    30361 atcacggaaa acgccgtcga caaggcatcg gcaaccgccg catcatccgc cattacgctc
    30421 atgcttttat aacgcggcgt actcacgccg gttcgcggat cgaacaaatg ggtaagcctg
    30481 cccgctttgt ccatcaccgt cccatagccg cccgaagtgg caaacgcctt gtctttcatc
    30541 gtgatatttg ccaacacgcc ttcttcgtcg tccggattgc ggatgccgac attccacatc
    30601 cgcctgccgt ttgtatcaaa ccccctgatt tcgcccatat cgaccagcgc ggcaggcact
    30661 ccgtttgctt taagcagcgc aacaaccttg tccgtgatat agccttgcgc gatgccgttc
    30721 aaggacaaac ccataccctt ttcggcaaaa cggatttcac ggtcgtcaaa cgagactttg
    30781 ccgaagccga cccgtttcag ggtttccttc acgctattct ccggcggcgt ttccgcatcg
    30841 ggacggccgg caaaataatc ggcatacagt ttccacaacg cctgaaccgt cggatcgaac
    30901 gcgccttccg tcagcgcgtg aatatcgcgg ccaggctcaa cagttccaaa aaatccgccg
    30961 gaggcgaagt cagataaccg tcgcggttca gacggctgat caggctgtct tcacggtaaa
    31021 ggctgaacat tttttccaaa cgcgccactt ccgccaaaac cttattgacc aaatccgccg
    31081 cctgtctgtc gtccacgccg aacaggcgca gctccgcgcc ggaacccagc gcgacacctt
    31141 tccagaaaaa cacattttca tcgcgttttt tatccatgtt gcgtttttcg ccgccggcgg
    31201 caaaaggatt cggcaaaaaa gaaaccgccg caccggccac agcaacggcg gcagccgtca
    31261 gaaaacgtct gcgcccgaaa tgcctgccca taccgcctcc taaaccgaca ctaccgcctt
    31321 gatgtgcgga tgcggatcgt agccttccaa ctcgaaatct tcaaacttga aggcaaacaa
    31381 atccttgact tcaggattga ttttcatcac aggcaaggcg cgcggttcgc gttccaactg
    31441 caatgcggcc tgttcgaaat ggttacggta caaatgcgcg tcgccaaacg tatggacaaa
    31501 ctcgcccgcc tccagcccgc acacttgcgc catcatcatc gtcaacagcg cgtagctggc
    31561 aatattaaac ggcacaccaa ggaaaatatc tgcactacgc tggtaaagct ggcaggacag
    31621 tttgccgtcg gcaacgtaaa actgaaacag cgcgtggcag ggcggcaagg ccatctcatc
    31681 gaccaaagcc ggattccacg ccgacacaat caggcggcgc gagtcggggt ttttcttaat
    31741 ttgttccacc acattggcga tttggtcgat atgcctgccg tcgggcgcgg gccagctgcg
    31801 ccattggtag ccgtaaaccg gacccaagtc gccgttttcg tccgcccact cgtcccaaat
    31861 ggaaacattg ttatccttca ggtatttgat attggtatcg cctttgagga accaaagcag
    31921 ctcgtggatt atcgagcgca aatgcagctt tttggtcgtc agcagcggaa aacctttgcc
    31981 caagtcaaaa cgcatctgat aaccgaatac ggagcgcgta cccgtaccgg tgcggtctga
    32041 tttgtccgta ccgttgtcga ggacgtggcg catcaagtcc aaataggcct tcatcatcat
    32101 ctttcatcaa attaaacggc acatattgta acatttccgg ccaacacccg agtccggaca
    32161 aacgcgcagg ccgccccgcc ggacgctcaa aacgcggatg aaggcaggca ggattgttgg
    32221 caatttcagc cctttttcac agcaaaagca ggcgtgaaaa caaaattatc ttgattggaa
    32281 tcaaaaaatc tagtttaact acttagaata aaattccaat aatatttttt atttgagaaa
    32341 ttaatttatg attattggtt gattatcggc aataaacatc atatatcgaa aatatgggaa
    32401 aaataatgtc aacaatttgt gcaaaattag gcttggcatc agaaaaaaat aggtttatat
    32461 tgccaactac aaatttgttt tcccattagt acaatatcaa ccaaaaggag tatccgaatg
    32521 actgacctga acaccctgtt tgccaacctc aaacaacgca accccaatca ggagccgttc
    32581 catcaggcgg ttgaagaagt cttcatgagc cttgatccgt ttttggcaaa aaatccgaaa
    32641 tacacccagc aaagcctgct ggaacgcatc gtcgaacccg aacgcgtcgt gatgttccgc
    32701 gtaacctggc aggacgacaa aggacaagtc caagtcaacc gcggctaccg cgtgcaaatg
    32761 agttccgcca tcggccctta caaaggcggc ctgcgcttcc acccgaccgt cgatttgggc
    32821 gtgttgaaat tcctcgcttt tgaacaagtg ttcaaaaacg ccttgaccac cctgcctatg
    32881 ggcggcggca aaggcggttc cgacttcgac cccaaaggca aatccgatgc cgaagtaatg
    32941 cgcttctgcc aagcctttat gaccgaactc taccgccaca tcggcgcgga caccgatgtt
    33001 ccggccggcg acatcggcgt aggcggacgc gaaatcggct acctgttcgg acaatacaaa
    33061 aaaatccgca atgaattcac ttccgtcctg accggcaaag gtttggaatg gggtggcagc
    33121 ctcatccgcc ccgaggcgac cggctacggc tgcgtctatt tcgcccaagc gatgctgcaa
    33181 acccgcaacg atagttttga aggcaaacgc gttctgattt ccggttccgg caacgtggcg
    33241 caatacgccg ctgaaaaagc catccaactg ggtgcgaaag tgctgaccgt ctccgactcc
    33301 gacggcttcg tcctcttccc cgacatcggt atgaccgaag cgcaactcgc cgccttgatc
    33361 gaattgaaag aagtccgccg cgaacgcgtt gccacctacg ccaaagagca aggtttgcaa
    33421 tactttgaaa accaaaaacc gtggggcgtt gccgccgaaa tcgccctgcc ctgcgcgacc
    33481 cagaacgaat tggacgaaga agccgccaaa accctgttgg caaacggctg ccacgtcgtt
    33541 gccgaaggcg cgaatatgcc gtcgactttg ggcgcggtcg agcaatttat caaagccggc
    33601 atcctctacg ctccgggcaa agcctccaac gccggcggcg tggcaacttc gggcttggaa
    33661 atgagccaaa acgccatccg cctgtcttgg gcgcgcgaag aagtggacag ccgactgttc
    33721 ggcatcatgc aaagcatcca cgagtcctgc ctgaaatacg gcaaagtcgg cgacaaagtg
    33781 aactacgtca acggtgcgaa catcgccggc ttcgtcaaag ttgccgatgc gatgctggcg
    33841 caaggcttct aagcaaacgc ctccgccccg caaacaaaat gccgtccgaa ccgcaaatgc
    33901 tgttcagacg gcatttcatt atccgcccgt tcgaatcggg taatactata gtgaattaaa
    33961 tttaaatcag gacaaggcgg cgagccgcag acagtacaaa tagtacggca aggcgaggca
    34021 acgccgtact ggtttaaatt tagttcacta taccgataca tccgaatatg ccagaccgtc
    34081 tgaacggtat tcatacctcc caatcctgca cgcgcttcaa atcgttttgc gccaaagtat
    34141 ccgcgtggcg gttacggctc tgatattccc tgtctttcaa tatgctgctc gccacataat
    34201 tcaaatgagc ctttgccgcc tccgaagcct cgcccggccg gcggttcgat atcgcctcat
    34261 acagcacgcg gtgctgcgcc atcagtttcg gacgcggatc ttcttcctga ttcagataaa
    34321 taaggctgct gcgcgtctgc cggtacagca ttttcaacaa accgcccgac aaatggctga
    34381 acaacaaatt gtgcgccgca tccgcaatcg tctgatggaa gctgacatcg gcttcgccct
    34441 gatgttccag attgccgctt ccgcacgctt cctcaaactt ttcaagccaa aaccgaatcc
    34501 gcttcaaatc ggcatccgtc cggcgttctg ccgccaatgc cgccatacag ccctcgatgt
    34561 ggcagctgaa atcaaaaaca tcctgttccc aattggaatg tttgcccaaa agctcctgcc
    34621 aactttgcaa aaaatcctgc tgcggcttga ccgaaacata ataaccatcg ccctgcctcg
    34681 cttccaaaat ctgacgggca accaaaatat tcaatgccga ccggaccgac ggacgcgaaa
    34741 cgccgaactc ttccgccaaa acgcgttcgg gcggaatctt gcccccttcc gcgtaaaccc
    34801 cttccacgat acggccttcc aataccgaca atacctgatc gctgattttc tgaggcctta
    34861 ccagtttcat cactcctcct ttataaagat tccctgcaga acccttccga aatacatcaa
    34921 acatcaaatt ggactggcca atcagggcgg attctaatga cacgccgttc ccgccgtcaa
    34981 cggcacttac ctcgcaccgc ccccgaaaca caggaaaaaa ctacaccaaa ctacaatttt
    35041 tgttcatgca aatatttgtt ttaacgggat ttaaatgaaa gctccgattc aaatctgccg
    35101 aaccgcccaa aaatatattg acctgaatat taaagtttcg taaagtaatg caacgttgct
    35161 ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcggaga tgttcaatta
    35221 tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg actgccgccg
    35281 ccgcactctt acccatcgtc tttttctttg ccgcgctgac cgtcctgaag ctgaaaggct
    35341 atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgtattc ggcttcggga
    35401 tgccgacggg tatggcggtt tcttccctgt cgccgcagcc ggattgaccc aacgccccat
    35461 acgctacact ggacttgacc gcgcattaca aaatcggtaa atccacccgc atcggtttgg
    35521 actttgaaaa cgtgttcaac aaacgctacc gcacgatgcc ggatattcat gtttacggca
    35581 cgccgcgcag cctgaccgca accgtcaaac ataccttcta aacagatacg gcctgccgtc
    35641 ttaaaaggca gaccggcacc ggaaaccgtt ttaaagaaag aaatgccgtc tgaaacctta
    35701 tcgtttcaga cggcatttga ttgccctggc ggttatttgc cggtttgggt atcccgtttc
    35761 aatccgccgc cgagcgcctt gtacaaatcg gcaaggtttt cggcacgggt cagttgtgcc
    35821 gacaaagccg caccctccgc cgaatagctg ctgcgttccg catcgagcaa gtcgagcgcg
    35881 ccggatacgc cgtgtttgta acgcaggccg accaagcgca acgcttcttt agaggcgcgg
    35941 ctttgtttgc ttaaagcggc ataggcttta tccagctgct cgcgcgccgc caatgcgttt
    36001 gccacgtctt gaaaggcgga ttgtacggcg gattcatagg caacgatttg tgcctgttgg
    36061 cgcagcttgg ctgcatcaag gttggctttg ttcgtacccc aggtaaaaat cggcagggca
    36121 atagacggcg caaacgacca aacgcccgtg ccgcttttga acagcccgcc caattcggca
    36181 gaacccgtac cgacgcttcc ggtcaggcgg atggatggga aaaaggcggc gcgtgccgca
    36241 ccgatattgg cgtttgcctg tttgagcgcg tgttcggcgg cgcgtatgtc gggacggtcg
    36301 agcaatactt cggaactcaa accggccggc agcttctcaa caaaaaactg cttgtccaac
    36361 ggcaaaccgg cgggcaggtc gtcgggtatc ggttggttaa tcagggttgc caaggcattg
    36421 cgcgcctgct cgcggctgcg cgcggcatgg gcataatcgg ctttggcgga ttcgatcagg
    36481 gcttcctgct gacgcagggc gacggcggaa attacgcctg ccttataccg caattcggac
    36541 agcttgtagg tttcctcgcg cgttttcaaa acacgctgcg ccaaagacat cgcttcttcg
    36601 gcataacgct cgttaaaata ggctttggca acggtggcaa tcaggctcaa atgtgccgca
    36661 tcgcggttgg cggtgctggc aaaatagcct tgcagtgccg cctcgctgct gctgcggacg
    36721 cgtccgaaca ggtcgagttc gtaagatgcc gcacccagtc cgacattgta gctgctgctg
    36781 acattgccgc cgctcaaact gccttggcgc gagccgttcg cattggcggc aagcgtgggc
    36841 agaaggttgt tgcgctcgat catgtattgt ttgcggtaga tttcgctgtt caatacggct
    36901 gtacgcaaac tggtattgcg ttcgagtgcg atgtcgatca gcttttgcag gcgcgggtcg
    36961 gcaaaatagt cgtgccaacc caaatcaacc gcgcggatgc cgctgtcggc ggtatcgttt
    37021 ttaaacgttt ccgcaacttc gactttgggc tgctcgtatt ggggaatcat ggtgcaggca
    37081 gacaatgcaa aagctgctgc aacagaagtc aaggtagttt tcaatatagt atccataaaa
    37141 aaagtcctga tgccgtctga aaacccgtgg gcgttcagac ggcatggttg cttaatgttg
    37201 gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca tttcgtgctc
    37261 gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa caaggaacac
    37321 ggataaaagc gtgccgatga gcatccccca gaatacggtc gtgccgatgg cgcgctggct
    37381 ggcagaactt gcaccaccgg caatatacag gggaaccacg cccaaaataa aggcaaacga
    37441 agtcataata atcggacgga aacgcaggcg ggccgcttcc aaagcggctt cgaccgcgct
    37501 tttcccttgc gcttgaaggt ctttggcaaa ttcaataatc aaaatcgcgt ttttcgcact
    37561 caaacccatc acggtaacga aaccgacttg gaagtagatg tcgttggcaa acgagggaac
    37621 gctgcccaac aatccttcaa acaggttacg cccggttacg cccgcagccg cgcccgccaa
    37681 gcccaaaggc atcaccagca gaacggccag cggaatagac cagctttcat aaagtgctgc
    37741 aagcaccaag aagacggcaa cggcggcaag cgcgtaaagg gcaatggttt gcgaaccgcc
    37801 ttttgcctct tcgcgcgact gtccgcccca ctccaggctg taaccgctgc ccaattcgtc
    37861 aaccattttt tgaactgcct ccatggcctg accggtggaa acaccggttg cgggcgaacc
    37921 ggacagttcc atagcgggat aaccgttgaa acgaacgctc tgttctgtac ccatctgcca
    37981 agaaacggtg gcaatcgaag aaagcggaac ggcaatgccc gagctattcg gcacagtcag
    38041 attcaagatg tcggcaggct gcatacgggc atccccgtct gcctgcacca tcacgcgctg
    38101 cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcgc ttgccaatgc
    38161 ggtgcggatg tcggcaaacg aaacgccttg cgccgccgcc gcggcacggt tgatgtcgat
    38221 tttcaactgc ggcgcgtctt ccaaaccgcc ggcacggacg gtacttgggt taaacaaacc
    38281 gctggtacgc attttctgaa tcaactcgtt gcgtttggcc agcaatgcgg catgaccggt
    38341 attgttgcgg tcttgcaggt tgatgctcaa accggaaccg ttgcccaact ccataatcgg
    38401 aggcggaacg atggcgatgc cgaaaccgtc tttaagcgtc cccatcatca taccggtcaa
    38461 tttgcctgca accgattccg catcgctgcc cggggtttta cgctcgttcc agtctttgag
    38521 catggcaaaa cccattgcca tattctgacc gctgcccgaa aagctgaagc cggaaacggt
    38581 aatgatgttt tcaatttcag gaatactttt tgccaactgc gttacttgcg ccagcgtcgc
    38641 atcggtgcgc tctttggtcg cacccgcagg cagttgcacg ctgaccataa cgaagccttg
    38701 gtcttcggtc ggcaggaacg aagtcggcag gcgcataaac aggaatacgc ccacaaccgc
    38761 caaaccaacg taaacaacca tcatgcgcaa agtcttgcgc aatactttgg caacccagcc
    38821 ttcgtagccg tgtgtccagt tgtcgaattt tttgttaaac caaccgaaga aacctttttt
    38881 ctcttcgtga tgccctttcg ggatggtctt caacattgtg gcacacaaag caggtgtgag
    38941 tgtcagcgca aggaaggcag agaatgcgat tgatgccgcc atagtcagtg caaactgttt
    39001 atagatattg cccgttgccc cgctgaacat agccagagga acgaatacag acatcaaaac
    39061 agcagtaata ccgatgaccg caccggaaat ctgacccatt gcttttttgg ttgcagcctt
    39121 aggcggcagg ccttcacccg ccataatgcg ctcgacgttt tcaaccacca caatcgcgtc
    39181 atcgaccacg ataccgatga ccaaaaccat cgcaaacatc gttaatacgt taatcgacat
    39241 gcccatataa gagatgaagg cgaaaccgcc caacagggaa atcggtacga cgatggtcgg
    39301 aatcagcgta taacggatgt tttgcaggaa gaggtacatc acgacaaaaa ccagcaccat
    39361 cgcctctaac aaggtatgga tgactttttc aatcgaaatt tcgacgaatt tggaagtatc
    39421 gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg ccatgcgttc
    39481 tttaaccgcc tttgccgtcg ccatcgcatt accgctgttg gacagcatca ccgccatacc
    39541 ggtggtattt accccgttca gacgggttga ggaagaatag tcttccatac ccaatccgac
    39601 tttcgccaca tccttcaggt aaacattaga accatcagta ttggcgcgga ggatgacgtt
    39661 gccgaattct tctgccgtac tcaactgccc ttgcgccgtt acggtagccg taacagtctg
    39721 cccctgaaca gcgggaagcg aaccgatgga acctgcggaa atctggatat tctgcgccga
    39781 cagcgcgctg ccgatatcgg caaacgacaa attgtagttc tgcagtttct taggatcgac
    39841 ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc cttcgatacg
    39901 ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcctcgg ttgactgtac
    39961 gtctgacgac agcatcacaa tcatcaaaaa attggaacgc gctttggata cggtaacgcc
    40021 gtattgctgg acggtagcag gcaacatgct caatacttcg gaaagcttgt tttgcacctc
    40081 cacctgagcc aaatcttcgt cggtttcagg cgtaaaggtc aggctcacgc tgccgctgcc
    40141 gctcgaatcg gcagaagtgg acatataatc caaaccttcc acgccgttca tattccgctc
    40201 gataacggaa agtacgctgt cttccatcac ttgtgcggat gcacccggat aagtagccct
    40261 cagggtaata ttcggtgcgg caacagacgg atattgcgaa acaggcaggc ttttaatgcc
    40321 gaaaataccc gccgcaatga tgaaaatcga aataacccac gcgaaaatgg ggcggtcgat
    40381 aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcaggttt ggcttcagat
    40441 gccgtctgaa cgccggattg aggcgcagca gcttggtttt cagacggcat ccattcttta
    40501 ggcgttacct ttttcgcacc cgtcatgccg gcgatactga tgccttccac aaccaccttg
    40561 tccccgtcct tcagacctga cgtaacaatc caattcgcac cctgctgttg tgcaatcgtt
    40621 acctcgcgtg gttccatacc gccttgggcg ttcacaatca tcacggtatc tttcgtaccg
    40681 cgcgttaccg cctgctgtgg aacgatgaat gcattatcca ccgccacttg atccatcagc
    40741 acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc ggcgcgcagg
    40801 gtaatctgac cggtcgattc gtcaacggtc gggtcggcaa acagcaggcg gcctttttca
    40861 ggataaaccg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc cgccgccagc
    40921 agcttgccct cggctatctg acggcgcagt ttcatcactt cggatgcaga ctgggtaacg
    40981 ttcacataca tcggattggt ttggcggatg gtggctaaaa cagttgtatc gcccgcattc
    41041 aacagcgtac cttcggaaac tttggactgg ccgataaagc cggaaatcgg cgcggtaatg
    41101 cgcgaacggt tcaggctgat gccggcagat ttgatcgccg cctgcgccgc tttaacgcct
    41161 gcttcggcag aacgtttcgc cgttaccgcc gcatcgtact cttgtttact gatggcatcg
    41221 gcggcaacca gcggtttgta acgcgccaaa tccgcatccg ctttggcaag cgttgcctgt
    41281 gccgttgcca gttgcgcgcg cgcgctttcc agacttgctt cataagtgga actgtcgatc
    41341 tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg tttttggata
    41401 atgccgccaa cttgcgcacg gacatcggcg gtacgcagcg attccaaacg tcccggcaac
    41461 tcgacggtca acgcaacggt ttgcggatgg acggtaacaa cgccgacgac gggagccgga
    41521 gcttcccgac cagcaggctg cccgccctgt gccgcttcat ctgctttgcc gcaagatgac
    41581 agtgccaacg cgacagaggc agccaacgcg gccgcacgca tcgccttaga agcataaaaa
    41641 gccattatct atcctatctg tatgatttta ttaaaggatt ttagatttaa cagaaattcc
    41701 cgtttcaaaa tacaaaaccg cctgcttcgg catccctgta ttcaaacggg ctgcaaagca
    41761 ggtgggttcg gcacttcggc actgggaaac tgtctcaatt tcaggggcgt tattatacat
    41821 acacgattac acggatacaa agtctttttt ataatcccaa ccgtcaaacc gcgacggaac
    41881 gaaaacgcca ttatgagaaa aaccaaaacc gaagccttga aaaccaaaga acacctgatg
    41941 cttgccgcct tggaaacctt ttaccgcaaa gggattgcgc gcacatcgct caacgaaatc
    42001 gcccaagccg ccggcgtaac gcgcggcgcg ctctattggc attttaaaaa taaggaagac
    42061 ttgttcgacg cgctgttcca acgtatctgc gacgacatcg aaaactgcat cgcgcaagat
    42121 gccgcaaatg ccgaaggagg gtcttggacg gtattccgcc acacgctgct gcactttttc
    42181 gagcggctgc aaagcaacga catccactac aaattccaca acatcctgtt tttaaaatgc
    42241 gaacacacgg agcaaaacgc cgccgttatc gccattgccc gcaagcatca ggcaatctgg
    42301 cgcgagaaaa ttaccgccgt tttgaccgaa gcggtggaaa atcaggattt ggcagacgat
    42361 ttggacaaag aaacggcggt catcttcatc aagtcgacct tggacgggct gatttggcgt
    42421 tggttctctt ccggtgaaaa tttcgatttg ggcaaaactg ccccacgcat catcgggata
    42481 ataatggaca acttggaaaa ccatccctgc ctgcgccgga aataatcaag ccttggtaac
    42541 aatgccgtct gaaacaaaca aaccctttca aacggcatca aaatgacaca aagcattctt
    42601 ctaaaaatac atattcacta aattgcattt ttaatttccc ctatcatcgc atgaacattg
    42661 tcttggtcaa aatgtccgtt ttcttctgaa taaacttcta acaaataatg ttcaatgaac
    42721 gttttatctg tcatcaacga tacatctttg gcaatgtctt catacgactc aaaatcatct
    42781 tcatgccatg gatcatattt atccatgatt ttttgaattt catttttcat atcatttacc
    42841 ttccaatatt tatttacaat taataacaat accattcgaa tgtaaactgc atttttctcc
    42901 ggcattcttg caaacaaaaa ccgaaaatcc cgtcattccc gcgcaggcgg gaattcgttt
    42961 ttttgagttc cagtcattcc tgataaggct ttaacgtcaa gttttcggat tcccgccttt
    43021 atgaaaataa cgatgtgggc attttccgtt ttaatctatt acggttatat acatatacga
    43081 ttattttagt ttgcttacaa aacacttcat gttaaattca aaaatttaat gcactcaata
    43141 tattttttta aggagaagca gatgagtcaa accgacgcgc gtcgtagcgg acgattttta
    43201 cgcacagtcg aatggctggg caatatgttg ccgcacccgg ttacgctttt tattattttc
    43261 attgtgttat tgctgattgc ctctgccgtc ggtgcgtatt tcggactatc cgtccccgat
    43321 ccgcgtcctg ttggggcgaa aggacgtgcc gatgacggtt tgattcacgt tgtcagcctg
    43381 ctcgatgccg acggtttgat caaaatcctg acgcataccg ttaaaaattt caccggtttc
    43441 gcgccgttgg gaacggtgtt ggtttcttta ttgggcgtgg ggattgcgga aaaatcgggc
    43501 ttgatttccg cattaatgcg cttattgctc acaaaatccc cacgcaaact cactactttt
    43561 atggttgttt ttacagggat tttatccaat acggcttctg aattgggcta tgtcgtccta
    43621 atccctttgt ccgccgtcat ctttcattcg ctcggccgcc atcctctcgc cggtctggct
    43681 gcggctttcg ccggcgtttc cggcggttat tcggccaatc tgttcttagg cacactcgat
    43741 ccgctcttgg caggcattac ccaacaggcg gcgcaaatca tccatcccga ctacgtcgta
    43801 ggccctgaag ccaactggtt ttttatggta accagtacgt ttgtgattgc tttgattggt
    43861 tattttgtta ctgaaaaaat cgtcgaaccg caattgggcc cttatcagtc agatttgtca
    43921 caagaagaaa aagacatccg gcattccaat gaaatcacgc ctttggaata taaaggatta
    43981 atttgggcag gcgtggtgtt tgttgcttta tccgccctat tggcttggag catcgtccct
    44041 gccgacggta ttttgcgtca tcctgaaaca ggattggttg ccggttcgcc gtttttaaaa
    44101 tcgattgttg tttttatttt cttgttgttt gcgctgccgg gcattgttta tggccggata
    44161 acccgaagtt tgcgcggcga acgggaagtc gttaatgcga tggccgaatc gatgagtact
    44221 ttgggacttt atttggtcat catctttttt gccgcacagt ttgtcgcatt ttttaattgg
    44281 acgaatattg ggcaatatat tgccgttaaa ggggcggtgt tcttaaaaga agtcggcttg
    44341 gccggcagcg tgttgtttat cggttttatt ttaatttgtg cttttatcaa tctgatgata
    44401 ggctccgctt ccgcgcaatg ggcggtaacc gcaccgattt ttgtccctat gctgatgttg
    44461 gccggttacg cgcccgaagt cattcaagcc gcttaccgca tcggtgattc cgttaccaat
    44521 attattacgc cgatgatgag ttatttcggg ctgattatgg cgacggtgat gaaatacaaa
    44581 aaagatgcgg gcgtaggcac gctgatttct atgatgttgc cgtattccgc cttctttttg
    44641 attgcatgga tcgccttatt ctgcatttgg gtatttgttt tgggcctgcc cgtcggcccc
    44701 ggcgcgccca ctttgtatcc cgcaccttaa acacgataaa caaaataccg tctgaaaacc
    44761 ttttttctgt tttcagacgg catttgcctt tccatcccgt caggcttctc cggtcccttc
    44821 cctcttttcc gctgcggcaa gcgtgtcggc gagcaggcgg gcgaggtttt caaacagggg
    44881 ctgttcgagc gggttttggc ttgcgttgcc gaacacgagg gcgacttcgg tatagtcttt
    44941 ctgccctttg ctgactttgc tgccgtaata ggcggtttgg gcttttttca gggcggcttc
    45001 ccaatcttgt ttttgggcaa acgcttcggc ggcggcaagc gaggttttgg caaaaaacgg
    45061 cagcgggcgg ttttgcccga tattgaaaaa cgccatccat tccgacaaca gctgcaatgc
    45121 cctgtcttgc gcgatttccg ccaatacttc tgtttctccg gattggacga taaaggtttg
    45181 ccgcgtttcg gcttcagacg gcataacggc gcaaaatatc aggtgttcca acaaaaaagc
    45241 cacacgttgc ggcgcattgg gtttgccgta ggcgtaaaac acctgaccgc agcggtacag
    45301 attgcccaag ctgcctttca ggatttgccc gtccgacggt atggcgtatg ggaacggagg
    45361 aagttttggg ctgtttaaaa ccgccgtgtc gatttgtttg gcggcggttt ggaagtcctg
    45421 ctgccaaagt ctgcccaact ctcccgacgg caggaggctt tccgccccga tgcgggcggc
    45481 ggtttgggca aaatcttgtc cttcgcgccg tgcttcgatg taggtttcgg cgatttgatc
    45541 ggcgtgttgc ggatcgaagg gttcggcagg ttcccaggct tcgccgatat ggggttcgct
    45601 ccacgcaagc tgctgctgaa gccatacttt gacggggttg cgccagaagc ggataaattc
    45661 gtcctgtccg atttcggcaa cgggttcggc gttttctacg ggttggtcga aaaaagggcg
    45721 cggcggttcg ggcgtttgtc cgagcgcggc ggcgtagtcg gtacgcgtgc cgaatatgcc
    45781 gtctgaacgt ccgccttctt ggaaatatcg gcgcgagaag gcttgcagcg gatgctgttc
    45841 tatccaattt tgtgcaagtt ggcggctgtt tgtgccggtc atggcggcaa ctgtgtcgat
    45901 gagttcgccc aacagggaag acggggcaag ctcttcgtct ttgcggatgt cgcgcccgat
    45961 gtaggacaaa tacaggattt cgcgcgcgct gatgagggct tcgaggaaca ggtagcggtc
    46021 gtcgtcgcgg cgggcgcggt cgcctttggc gggatgtttg gcaaccaggt cgaatacggc
    46081 ggctttggta ttgcggggaa aatctccgtc gttcaaaccc aacaggcaga tgactttgaa
    46141 cggcaggctc cgcatcggca ccatactgca aaaggtgatg ccgccgcgta aaaagcctgc
    46201 ctcgctttcg ctgtcgagga agcggcggat atggcggatg acggtttgtt gcggcaactg
    46261 tccgaaaaac ccagccaaag tgctctcttc ctgccatttg acccattcgt tttcgaggtt
    46321 ttggactgcc ttttggtcat cgggcccggc ttgaaacaat gtttcaagca aatcccggca
    46381 acgcgccacc cattcgccga ccgtcgcagg ttgccgccat atccgggcaa tatccgacag
    46441 ggtttcgagg aaggcggcaa aacgtccgaa cagggcggtt tggttcacgt cggcatacca
    46501 cgcgctgaca tcctgccaca tcgggttgcc gccttcgggc agcatccagc ccaatatcat
    46561 acggtctgcc gcctgcttcc aggtaaacag ctgatccgtg ccgccgcgca tttctccgtc
    46621 caaaccccag cggacgttca aatcggcaac catgtcgtgc aaaagcggta aatcgtcccc
    46681 ggtcagtccg aaacggcgca acacgggcgc ggtttccaaa agcgcgagca ctttgccgac
    46741 ttcaaaacgg ctttccagca agtcggacac gcactccaac gcataaaaaa acggttgccg
    46801 gcggctgatt ttcacgtccg aaacggaata cggcaatgcc tgcgcgccgg gctgcgcctg
    46861 tccgaacacg gcttcgataa aaggcgtata gggttcgatg ttcggggtta atacggcgat
    46921 atcatgcggc tgccaatcgg gatgttcatg cagaattttc aacagcttgt ctttgagtat
    46981 ctgcaattcg cgcaaagggc tgtgtgcgga gacgatgcgt atcgagccgt cgcccgtgtt
    47041 gacgcttccc acattttcag acggcatttt caggttttga atatcggttt gcagggcgtg
    47101 caaaagcgta tcgcgtccgc cttcttcaaa taccggcgtt tcgccttcta tttccatttc
    47161 gttcaaaaag tcgaaaaagt cgcgcccctg cttgcccaat gaggcaagca gcggatgccc
    47221 tgcctgcgtc agatcgggat cgccgccgcc tttgaggatt tgcgccgctt cgatgacgtt
    47281 gccccaatgc atcccgctcg gattgagtgc gaacacgaac acgtcgcaat gttcggacag
    47341 cttgtgcaaa agctgcaaat acatcggcgc catcgtggaa atgccgaaca cgaaataacg
    47401 ctcgggcagc ttatccttgt ccaacgcggc aagcagtttt tcccacaacg cgacacggtg
    47461 cggcgcgctc tgcctgccgt cgtcgaggta acgccacagt ttggactgcc agatttcgtc
    47521 gtcgcccaaa ccgagtatcc tgccctgctg ccaagcgtct atccactgag gacggtacac
    47581 gaggtattgg tcgaatatgt ctgcaagctg tcccgcaagc tggtaatccg ccgattctcc
    47641 gctgcccaga tagtcttgca gcacattcct cacatcttca aattccgctc cgttttgaaa
    47701 ttctgcgctg cggaacaaat ccagcagccg ccagcgcatg acttcgggtg caaacgggct
    47761 gagttccgga ataccgggaa tcagtttttt catcaacttc cacgtcaggc cggcgggcag
    47821 gctgaacgcc aaattcgccg ccacgcccaa atcgcgggcg aggcaggtgt tgaggtagcg
    47881 gcgcatcccc tggctctgca caacaatctg ttcgggctgc aaggcacatt tcagcggttt
    47941 gactttttga atgcgggcaa acagtgcagc gagcgattcg aggcggttgg attggtacag
    48001 gtaaaacatg gcttgctttt tcaaaatgac agtgatgcgg aattatatag cttctcgggc
    48061 aagccgcccg caaacaaaat gccgtctgaa cgcttcagac ggcatcgggt catttaaacc
    48121 atttcctcaa cacaggaatc cgcgacagca gcaaggtatc caacagccaa atcacggcaa
    48181 tggcaagcag cgcggtcggg aaaagcagtg caatcaacag cagcggcaat gccatcgccc
    48241 accagacggg cagtttgatt ttttgcgccg gaggaacaat gcccgccacg ccggacggac
    48301 ggcgtttcca ccacatcacg cagccgctga tgccgataaa aatcacggca aggcagaaca
    48361 cgacgttcgc caacacgctc caccagccca aagtccccat atgcagcgca atgcttgccg
    48421 ccataaattt gccgaacggg ttgtaatcgt caaaacggat gtcggcaaga atcttgccgc
    48481 tgtactggtc gatatgcacc gtgcggtcgg caaacgggct gatcatgtca taactcatag
    48541 aatcctgcga caaagtccat accccgtcct cgcctttggg caaattcaac tgataacgcc
    48601 ctttgaaacc gatttcccgc gcgaaacggt cgacggtttc caatgtcatc ggctcgctgg
    48661 ggttaatgcc gttttcaccc acagtcgtcc ctgagacagg cataggcgta agctccaaaa
    48721 tccacggcac ttccttaacc ttgccgtcat tcaatacctc gccgtgggtc ggcacgactg
    48781 aaacggggtt cggttcgaca ccccatttgc cggccgggaa ctgactccaa gcctgcacga
    48841 acttgccgcc ccaaatacct gcccaagcaa tacccgacag gcagaacaac agtaaaatca
    48901 acgataccca agttccaaac gcgccgtgca gattccgcca ccaagaacgc gccctgcctt
    48961 ttggcggcag cagcatcgct ttaatgccgc gctgtttcgc ccaccaaagg tacaagccgc
    49021 tgacaaccat aataatggtc agtgaagctg ccgtttccaa aagataatcg cctgccgcac
    49081 cgagcatcat atcgccgtgg atttcatcca tagtgtgata ccaaccctga ttgcgcggca
    49141 tggtgttgac cacttttgcc gtataaggat cgaccgcaac catggttgcc ttaccctcat
    49201 tgttgacacg gaacacagca accatatcat cggcacgcgg tgcaatatat tggacgacgg
    49261 acgaagtctc cggattaatg gcaccgcgtg ccgcttctgc ctgaacagag aggggttgca
    49321 ccactgcctg agggaaaacg tgaatacgct ctccctcttt accggtaata ttggcaaaca
    49381 acagcatacc caaacctgta acggcaagca gggtcaagaa gggcataacc aataaaccgg
    49441 cataaaaatg ccacctccaa acgctcagat aatgccggtt attctgctga ccggcttcag
    49501 ttttgatttg tgtatccatt aatcgtcctt ttgaaattaa ggctatcgtg atgatgcgcg
    49561 attataaaca ataaagacta attctttatg actaaaatca atttttttgc aacatatgaa
    49621 aaaagattca attttgaaga aacaaaccta tatgccgtct gaaagcatca aacaatgctg
    49681 tccgacaccc aactgcacca ttctttaaac catacaaaaa tatcagtcgg tctgactgct
    49741 taagcatcaa aatccccact ttcagacggc ataaatcaaa aacaggattt ccgttcaaaa
    49801 taaaaaatcc ggattcgata tgaatccgga tttctgcatc cgacaaagtc ggaaaacaac
    49861 ggttagaact taccgcctga ctggttgacc atatagtcaa ccgcagcttt gacctcatca
    49921 tcgctcaaat cgccgcgacc tccttttgcg ggcatcgtat taaaaccttc gatcgcgtgt
    49981 ttgtgcaacg tgtccttacc ttttttgatg cggtcggccc agtcggcttt ggtgcctaca
    50041 tggggaatac ctggaattgc attgccatgg caggcggcac aaacggtttc ataaacctgt
    50101 ttgccatccg ctttggcggc aggtgcggct ttttcctcgg ctttaggttc tgctgcggca
    50161 ggtttggctt cagaagcggc ttgtgctgct tctgcaggag cagatggtgc gggttctgcc
    50221 gccggtgcag gctcggcttt tttcaccgga gctttaccgt ctttattgga aagaccccat
    50281 acataagcag tcatgatatg cagtttgtct ttatccaaga aatgtcccca agcgggcatt
    50341 tggctgctgc gaccgttggt aatggtttcg ataatggatt tttgcgtacc gccccacaac
    50401 cacacgtcat cagtcaggtt cggacccaaa ccttggatac cttgtccctt atcgccgtgg
    50461 caagtgaaac agttggcagg cggaccgctg aacaaggctt gtccgcgcgc ggcacgttcc
    50521 tcatcatact gacctttggg ttttgaaagg gacatcacat aatgggcaac gtctttcacg
    50581 ccttcttcgc ccaaagcagg accccaggca ggcatagtcg caacacggcc tttttcgatg
    50641 gtctcgtgga ttttatcggg atcgccgccc cacaaccaat cgctatcggt cagattcgga
    50701 aaacctttag aacctttggc atcagagccg tggcactgga tacaataagt gttaaacagg
    50761 ttttgggcga tttgcttggc ctgtgggtct tttgccactt tttcaatcgg catatccgca
    50821 aacttggcat acagtttgcc gtattgctca tcggcttttt tgacctcttt ttcatattgg
    50881 ttatggctgg tccatttcag cagacctttg tagtcgccga cacccggata cataaccaga
    50941 taaccgatac cgaacagcca cgtcaaaaca cacagccaaa accaccagcg gggcagcgga
    51001 ttgtcgtatt cggcaatgcc gtcccactcg tgacccgtag tttgtacttc ttcgcccttc
    51061 ttcggacgtt tgacaacatt ttgagacagc agcagccaag ccaaagcgat aaagctcagt
    51121 aagacaataa ctgcaatata tatattccag aaattactgg taaattggga tgttgcgttc
    51181 attgttttgc tccgttatca caatattaac ggttttcgct tttcttatct tgcgcatctt
    51241 ggttttcagc aaaaatgctg tttgcggcat catcgtagtt tttcttattc cgcctgttga
    51301 agacgatata gagtactaac aggaaacaga taaagatcca taccgtgaaa agagcgcgaa
    51361 taccgttaat atccatgatg ttaccttacg tttttcaaag ccagacccaa tccttgcaga
    51421 taggcgacta cagcatccag ctcggatttg tttgccaaag cctcaggcgc tttcgcaatt
    51481 tcctcatcac tgtaaggagt acctacttta cgcaaagcct tcatgttggc aacggttgca
    51541 tcgacatcga ctttattgcg tgcaagccac gggaatgccg gcatattgga ctcaggcacg
    51601 acatcacggg gattcagcag gtggatgcgg tgccattcgt cggaatagcg gccgcccaca
    51661 cgtgccaaat caggaccggt acgtttggaa ccccattgga acggatggtc gtaaaccgac
    51721 tctccagcaa cagagtaatg accgtaacgc tcagtttccg cacggaacgg acgaatcatt
    51781 tgagagtggc agttgtaaca gccctcacgg atgtaaatat cgcgtccggc aacctgcagg
    51841 gcattgtaag gcttcacgcc cggcgccggc tgtgttgccg ccttggtaaa ggccaagggc
    51901 acaacttcaa tcaacagacc gacactgact acaagcagcg tgaacacaat cagaacgccg
    51961 attttttctt cagccaattg ttgtaatttc attttggtag cctatctttc ttagtatttt
    52021 agtggtgctg tgtttgggaa accgcaggga tttcggcatc gactgcttta ccaccgatgg
    52081 ctgtgcggta cacgttgtac gccataatgc acatgccact cagatacaat aaaccacctg
    52141 caaaacggat catgtagtaa ggcatggtgc gttttacgga ttcgacaaac gagtaggtca
    52201 gcgtaccgtc atcgttcaga gaactccaca tcaaaccctg catcacacct gcaatccaca
    52261 tcgcggcgat atacagaacc acaccgatag tcgcaatcca gaaatgcgcc tctaccagct
    52321 tggtgctgtg catctgctct ttgccgaaca gacggggaat catgtaatag acggaaccga
    52381 tggttacaaa gcctacccag cccaacgcac ccgcatgaac gtgcgcgacg gtccagtccg
    52441 tatagtggct caatgcattg accgttttaa tcgacatcat cgggccttca aaggtagaca
    52501 taccgtagaa ggacaaggat acaatcagga atttaagaat cgggtctgta cgcagtttgt
    52561 cccacgcgcc ggacaaggtc ataatgccgt taatcatacc gccccaagag ggtgcgaaca
    52621 aaatcaagga cagaaccata cccaaagatt gcgtccagtc aggcagcgca gtgtagtgaa
    52681 gatggtgcgg acccgcccac atataggtga aaatcaacgc ccagaagtga acgacggaca
    52741 ggcggtagga gtaaacgggg cgggctgctt gtttgggtac gaaatagtac atcataccca
    52801 agaagccggc agtcaggaag aagcccacgg cgttatgccc gtaccaccat tgaaccatag
    52861 catcaatcgc accggaatag acggggtatg acttcatcaa accggccggg atgctgatat
    52921 tgttgacgat gtgcaaaagt gcgaccgcca aaataaagcc gccgtagaac cagttggcaa
    52981 cgtaaatgtg ttttaccttg cgtttggcaa tcgtaccgaa gaatacgatg gcgtaagcca
    53041 cccaaaccaa agtaatcaga atgtcaatcg gccattccag ttcggcatat tccttacctt
    53101 gggtccaacc catagggaag ctgacgacgg cggcaacgat taccgcctgc caaccccaaa
    53161 aggtaaatgc cggcagccaa ccgccgaaaa gacgggtatt acaagtacgt tggacaacgt
    53221 agtatgatgt gccgatcaga ccgcaaccgc caaatgcgaa aataaccgca ttggtgtgca
    53281 gcggacgcag gcggccgaag tggaaccaag gtccgatatt agacaagtcg agggcaggag
    53341 caaaaagctg ggcggcgacg ataacgccga ccaacatgcc cacaatcccc caaactacag
    53401 tcatgatggc gaactggcgc accactttgt agttgtaagt ttgtgtgtcc atgagagtct
    53461 ccatgaatta tgggaataaa gatttttatc ctgccgcttc cgcagcctgt ttaaggtgca
    53521 atccgggcaa gcgtaatttt ttctaaattt aacatatctg ccttattacg ccaagcggaa
    53581 ttacattcgc gccgccggcg aaccctttgc ttaatctgtt ttttattaca tataaatcat
    53641 attgttataa taaattacaa cccggccgcc attgcttttg tttccaattt tccctttttg
    53701 tggcacttta ttgatgtagg ttaagctgca ttttaaaggc atttaatcca tcccgtttaa
    53761 cgatatattt gatagttatg attcattata aaatagcccc gtcccctcta gaccatgaat
    53821 ggcacatcct gctgaaattc acacaagata atgattttcc tatagaaata agccttccga
    53881 attgggtgcc gggcagctat ctgattcggg atttttcccg ccacatcact tctatccatg
    53941 catcctgcaa cggcacgtcc atgccgctcg aacaaattgc aaaaaaccgc tggcacactg
    54001 ccgccgtacg cggcgagtgg caaatccgct acaccgtata tgcattcgat ttgtcggttc
    54061 gaggttcttt cctgacgaca gaacgcggtt tttttgacgg atcgtgcctg tttttgaaag
    54121 tcgaaggaac ggaaacgctg ccgcaccgct tggaattgac aggcattcca cccgaatggc
    54181 gtattgccac aacgctgccg gaaacaggga ggcttgtctt tcagacggca tcttatgacg
    54241 aattgattga ccgacctgtc gagatgggct tgattgaatt tttagatttt gaggcggcag
    54301 gcattccgca cacgattgcc ttaaacggca tatatcccga tttcgaccgc gacaggctgg
    54361 tttcggacat caaaaaaatc tgcgaaacag aactggcgat gttctcctcc cctgccccat
    54421 ttgagaaata tttgttcctg ctccacgtcg gcgaccatat ttacggcggt ttggaacaca
    54481 ccgacagcac cgcactgctc gccgatcgcc acagccttcc gccgtacggt atgaccgatg
    54541 ccgacgatgc ctacaccaca ttgctcggac ttttctccca cgaatatttt cacgcgtgga
    54601 acgtcaaatc catcaaacct gccgcattcg ccccttatga cctcgacaaa gaaaactata
    54661 ccgaacaact atgggcattc gaaggcatta catcctatta cgacgatttg tttttggcac
    54721 gcagccgcac catctcgccc gaattttatt taaacctgct agcacaaggc attacgcgcg
    54781 tacaacaaac ccgcggccgt ttgaggcaga ccttggcgga atcgagtttt accgcgtgga
    54841 acaaatttta caaaccggat gaaaacagcc ccaacgccat cgtcagctac taccaaaaag
    54901 gcgcacttgc cgcattgtgc cttgatctga taatacgcaa ccgaagcaac ggcagacatt
    54961 cccttgatac ggtaatggac aaactctatc gggagtggag ggacacacac tcgggtattc
    55021 cggaaaaaca ctggcaaatc cgttgtcagg aaattaccgg cttggatttg acagattttt
    55081 ttcagacggc attgtacagc accgaagatt tgccgcttgc cgaatgcctg gcaaccacag
    55141 gcgtgaaact gaccttcctg ccgcttcccc gacaacacgg cggcggatat gcggaacaca
    55201 ttttccccat cccgccgacg ggcgatttcg gcgcacgctt caaacaaaac gccgaccata
    55261 tcgtcctgac ccatgtcttc aacggcggaa gtgcggaatc tgcggcactg tgcccgcaag
    55321 acaaaatcat tgctttagac ggttatgcct gcaccgactt tgccgcacaa tgggcccgat
    55381 accacgtcag ggcaaaaatc aatatccact tcttccgtgc aggcatattg cgtcaaaccg
    55441 tcttgacggt tcaggcaacg gcggcggata ctgccatcct acacatcaca gatcgcaacc
    55501 tgttggaaaa ctggttgttc ggttaaactt tcagacggca ttgcacacaa aatgccgtct
    55561 gaacaccaac cgcaaagcaa aggaaacaaa atggcgattc tcaaacttga cgaacatctc
    55621 tacatctccc cgcaactgac caaagccgat gcggaacaaa tcgcgcaact tggcatcaaa
    55681 accgtcatct gcaaccgccc cgaccgcgaa gaagaatcgc aacccgactt cgcccaaatc
    55741 aaacagtggc tggaacaagc aggcgttacc ggattccatc accaacccgt taccgcacgc
    55801 gacatccaaa aacacgatgt cgaaaccttc cgccaactca tcggacaagc cgaatatccc
    55861 gtccttgcct attgccggac cggtacgcgc tgctccctcc tgtggggctt ccgccgggcg
    55921 gcagaaggta tgccggttga cgaaatcatc cgccgcgccc aagcggcagg tgtgaatctg
    55981 gaaaacttca gagagcggct ggacaacgcc cgcgtctgat tacaagccga aacgtttaaa
    56041 ccacaccttc aagcggcatt ccaccgcaac ttgaaaaaga ggacggcaaa ccttactgcc
    56101 gtcctctgtc cttctccgtt tttacagtgg gtatagcgga ttaacaaaaa ccagtacggc
    56161 gttgcctcgc cttaactcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
    56221 ggttccgtac tatttgtact gtctacggct tcgttgcctt gtcctgattt ttgttaatcc
    56281 actataaaaa ttagaaatgc acattttcat tattctcgcg caggcaggac tccagactta
    56341 cccatttcag taatgtttga aaataaaaga aaaatcagat gtttgtattc ccgcctgcgc
    56401 agaaatggag acggtgctct gtcgtctcat ttttgtttta atcaactata tataactgat
    56461 taaacataag aaatgccgtc tgaaagactt tcaggcggca ttcgttcaag cgtcgaactt
    56521 tattgcgcct tggtttcggt tacaaaaccg attttggtaa ttcctgcctg acgggcggct
    56581 tctaaagctt tgtttacata atcgtattcc accgccttgt ctgccgcaat cgccacaatc
    56641 acgttttcat tctgctcctt ggcggctttc agacggcttt ccacttcccc gatttccact
    56701 ttgcttgcag aatccccgcc gacataatag ccgccgttcg catcaatcgt caggcgcagg
    56761 gggtctttag gctgtttgtc ctgcttgttt gcctgctcgg acgcggtcgg cagttccaaa
    56821 gggatggaat gcgtcagcac cggcatagta atcataaaca caatcagcaa caccagcatc
    56881 acgtccacca acggcgtaac gttgatgtcg gacatcggag aatcgtcgct ggaattcatc
    56941 gaaccaaatg ccataatcag ctatcctttt gattaagcag gcggacgtgc aaatcgtgcg
    57001 ccatcgcatc caaatcctgg gtcagtattt ttgtgccgcg attgaggaag ttgtatgcca
    57061 acaccgccgg aatcgccacg aacaaacccg ccgccgtcgc caccagtgcc tcgccaatcg
    57121 ggccggcaac cgccgcaata ctcatctgcc cgctttgccc gatattgatc agggcgtggt
    57181 aaatccccca aaccgtgccg aacaacccga taaacggcgc ggtcgcgccg atggaggcaa
    57241 gcgcggtcat cccgtaatca aaccggcgca taatctgcgc catactgttg cggatttgaa
    57301 tgaccaaata ctcgttcaac ggcaaagcct gcgccagttc ggacgcttcg tttcggcggt
    57361 agttgcggta agactgcaat gcctcttgcg ccagtttgga caaaggcgca tcgacggcgc
    57421 gcactttttc gaccgcgtcg ttcagcgaca aagtatcgcg catatgccgt ttgacggcgg
    57481 cattcccttt gcgcgcccga tacagcttga tgcagcgcaa gacaaccaga caccacgtta
    57541 cggtactcat caacagcatc aacacaaaca caccgatcag gacgggatcg cccgattcaa
    57601 acactaattt caaattcata atgattccaa cactgaaaaa accaatcaaa catccaagct
    57661 gccgcaaact gccgcgacaa ccgcctaatt caattcaaac ttgacgggga ctttaaactc
    57721 cgtccaggca ttggcttgaa aatgcccgtt ttgcgccgct ttgcgtgccg cattgtccaa
    57781 acgggaaaaa ccgctgcttt tcaccacttt gacggactcg acatgaccgc ccggagaaac
    57841 caaaacgctc aaaacaacca taccctgctc gtcattttcc atagaaagtg cgggataggc
    57901 cgggcgcggg atgctgccgt tggcgcgtaa aggattgcct ttgctgctgc cgacaccggt
    57961 tccgccgccg ctgccgccgg ctccttcccc gtgttcgcct ttggcaccgc cgcttccttt
    58021 accgctgcct tctccgcgcc ccgttccgtc gcctttggta ccggttccct taccttcccc
    58081 attaccctgc tcgctgccgg ctttggcaga agcattaccg gaatgttcgg caggtttttc
    58141 agacggcttc tctgccggtt tttccgccgg tttcggggca ggcttcgctt ccggttcggg
    58201 ttcgggctta ggtttttctt cgggtttcgg cttttcctta ggttgctgaa tatccgcatc
    58261 cgcctttttt gtaaccgccg gcttcaaaac cggcttgggc ggttcgacag gtttgggcgg
    58321 atcgggcgcg ggttgcggtt cgggcgcggc aggcgcgcct gcgccttcgg gtgcgccgcc
    58381 ccctccgcca aaatcgccca aatcgacaaa ttcgataaca tttcctgact ctatcacggg
    58441 cagcttgtgc gcctgccaga gcaatgccac tattgccaaa tgcagcagtg cgacggaaaa
    58501 cacgactgcg ggggttaaaa ttcgttcttt atccataatt cgggcataat aatagcaaca
    58561 attcctattt gcaacctatt ttttataatt tgttgtcatg tgaacgcctg ctccattcat
    58621 aggcaaaccg tgtttaaacg cggtattaca gcaaatcatc agataacggg ccggcagaaa
    58681 aaatgattcc gtctgatttc ttattccaat aaaatcaggt tagatgatat attgccgctt
    58741 ctgtctgtca gccgtttcgg gctgcacacc acatctgttc aaaggaaaac catgtttcaa
    58801 aattttgact tgggcgtgtt tctgcttgct gtcctgcccg tgctgctcgc cattaccgtc
    58861 agggaggtgg cgcgcggcta tacggcgcgc tactggggag acaacactgc cgaacaatac
    58921 ggcaggctga cactgaaccc cctgccccat atcgatttgg tcggcacaat catcgtaccg
    58981 ctgcttactt tgatgttcac gcccttcctg ttcggctggg cgcgtccgat tcctatcgat
    59041 tcgcgcaact tccgcaaccc gcgccttgca tggcgttgca ttgccgcgtc cggcccgctg
    59101 tcgaatctgg cgatggctgt tctgtggggc gtggttttgg tgctaactcc gtatgccggc
    59161 ggggcgtatc agatgccgct ggctcaaatg gcaaactacg gtattctgat caatgcgatt
    59221 ttgttcgcgc tcaacatcat ccccatcctg ccttgggacg gcggcatttt catcgacacc
    59281 ttcctgccgg cgaaatattc gcaagcgttc cgcaaaatcg aaccttatgg gacgtggatt
    59341 atcctgctgc ttatgctgac cggggttttg ggtgcgttta ttgcaccgat tgtgcggctg
    59401 gtgattgcgt ttgtgcagat gttcgtctga ctggctctca gacggcataa acgttccaaa
    59461 aaacgcggaa ggacatattg ccctgccgct tttttttggt tttacaatgc cctgtcgaaa
    59521 taaagcggca cgcctgattt ttcactcata ccgattaaat aatccatcgt cttccgaggg
    59581 aacgcgatgc cgcgcaccac ggtcagattc ctcaaaacgg ggaaaaccaa aatatcctcc
    59641 ataccgattc caccgttgat gccgtctgaa gtaccgtcca tcaaattctc caactcttgc
    59701 aaatctgcgt ttatccgttc aaggtattgg gcggttttat tcaaattggc ggaaaagctg
    59761 ccgatgcttt tctctttttt gtctgtgaaa tatttcaccg cttccggcgt tacaaattcg
    59821 ggcagcccga ttttgatcaa gcgtggctga accaatttgt tgttgtatcc gcctaccttg
    59881 tccagccacg cctgtatctc ggggcggact tcgtctttca gacggccttc tcggtcgaaa
    59941 tggcacacaa tgtccaaact ctcgcccata aacgaaccgt cttctttttg caggacgggc
    60001 acttgtttcg caccgatcat accgatcggc gttgcctcgt cgtcgtttgc cagcacgact
    60061 tcttcaacgt cagcaccaaa cagcccggca gccatccgcg cacgcacgca aaacgggcaa
    60121 tggtcgtaaa tatacagttt catcaaaata ttcctcgtca acctgtcggt accggctacc
    60181 ttaacacccg gcgccgcccg aaacaagttt atcttcccgc ctatgcaccg taaataaata
    60241 agctgttaca atgaactcgt ttttatcgga acggaagatc ccatcatgac cgccatcagc
    60301 ccgattcaag acacgcaaag cgcgaccctg caagaattgc gcgaatggtt cgacagctac
    60361 tgcgccgctc tgccggacaa cgataaaaac ctcatcggta ccgcatggtc gctggcgcag
    60421 gaacattatc ctgccgatgc cgccacgccg tatggcgagc cgctgcccga ccacttcctc
    60481 ggcgcggcgc aaatggtcga cgaactcgac ctgctgcccg atgccgtcgc cgccaccctg
    60541 cttgccgaca tcggacgcta cgtccccgat tggaacctat tggtttccga gcgctgcaac
    60601 agcaccgtcg ccgagctggt caaaggtgtg gacgaagtgc agaagctcac ccacttcgcc
    60661 cgggtggaca gcctcgccac ttccgaagag cgcgcgcagc aagccgaaac catgcggaaa
    60721 atgctgctgg cgatggttac cgacatccgc gtcgtattaa tcaaactggc gatgcgtacg
    60781 cgcaccctgc aatttttaag caacgccccc gacagccccg aaaaacgcgc cgtcgccaaa
    60841 gaaaccctcg acatcttcgc cccgctcgcc aaccgcttgg gcgtgtggca gctcaaatgg
    60901 cagctcgaag atttgggctt ccgccatcaa gaacccgaaa aataccgcga aatcgccctg
    60961 cttttggacg aaaaacgcac cgaacgcctc gaatacatcg aaaacttcct cgatatcctg
    61021 cgtacggaac tcaaaaaata caatatccac tttgaagtcg ccggccgtcc gaaacacatc
    61081 tactccattt acaaaaaaat ggtgaagaaa aaactcagct tcgacggcct gttcgacatc
    61141 cgcgccgtgc ggattctggt cgataccgtc cccgagtgtt acaccacgct gggcatcgtc
    61201 cacagcctct ggcagcccat tcccggcgag ttcgacgact acatcgccaa ccccaaaggc
    61261 aacggttata aaagtttgca caccgtcatc gtcggcccgg aagacaaagg tgtggaagtg
    61321 caaatccgca ccttcgatat gcaccaattc aacgaattcg gtgtcgccgc ccactggcgt
    61381 tacaaagaag gcggcaaagg cgattccgcc tacgaacaaa aaatcgcctg gttgcgccaa
    61441 ctcttggact ggcgcgaaaa tatggcggaa agcggcaagg aagacctcgc cgccgccttc
    61501 aaaaccgagc ttttcaacga cacgatttat gttttgaccc cgcacggcaa agtcctctct
    61561 ctgccaacgg gcgcaacccc catcgacttc gcctacgccc tgcacagcag catcggcgac
    61621 cgctgccggg gcgcgaaagt cgaagggcag attgtgccgc tgtccacccc gctcgaaaac
    61681 ggacagcgcg tcgaaatcat taccgccaaa gaagggcatc cttccgtcaa ctggctttac
    61741 gaaggctggg tcaaatccgg caaggccatc ggcaaaatcc gcgcctacat ccgccagcaa
    61801 aacgccgaca ccgtgcgcga agaaggccgt gtccaactcg acaagcagct tgccaaactc
    61861 acgcccaaac ccaacctgca agagcttgcc gaaaatctcg gctacaaaaa gccagaagac
    61921 ctctacaccg ccgtcggaca aggcgaaatt tccaaccgcg ccatccaaaa agcctgcggc
    61981 acgctgaacg aaccgccgcc cgtgcccgtc agcgcaacca ccatcgtcaa acagtccaaa
    62041 atcaaaaaag gtggcaaaac cggcgtgctc atcgacggcg aagacggctt gatgaccacg
    62101 cttgccaaat gctgcaaacc cgcgccgccc gacgatattg ccggcttcgt tacccgcgag
    62161 cgcggcattt ccgtccaccg caaaacctgc ccctctttcc gacaccttgc cgaacacgcg
    62221 cccgaaaaag tactggacgc aagttgggcg gcgttgcagg aagggcaagt gttcgccgtc
    62281 gatatcgaaa tccgcgccca agaccgctcc gggcttttgc gcgacgtatc cgacgcgctc
    62341 gcccgccaca aactcaacgt taccgccgta caaacccagt cccgcgactt ggaagccagc
    62401 atgaggttca cgctcgaagt caaacaagtc aacgacctcc cgcgcgtcct cgccggcctc
    62461 ggcgatgtca aaggcgtatt gagcgttacc cggctttaaa tacaaaaatg ccgtctgaaa
    62521 gccggataac gcttcagacg gcattttgat tgccgggttt tgctattttt tgttgtaata
    62581 atcaaatcgc acgttgacta tgtctttctc ggtaaaaata taacggagca tcgttgtgaa
    62641 tctttcataa cgttcatgaa ttcccacact atcaggcaac caaggggaag atttaattg
//
LOCUS       DAWTCI010000004        46165 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWTCI010000004 DAWTCI010000000
VERSION     DAWTCI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 46165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46165
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.004"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            423..1963
                     /locus_tag="WM481_000290"
     rRNA            423..1963
                     /locus_tag="WM481_000290"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2064..2140
                     /locus_tag="WM481_000291"
     tRNA            2064..2140
                     /locus_tag="WM481_000291"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2098..2100,aa:Ile,seq:gat)
     gene            2146..2221
                     /locus_tag="WM481_000292"
     tRNA            2146..2221
                     /locus_tag="WM481_000292"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2179..2181,aa:Ala,seq:tgc)
     gene            2568..5459
                     /locus_tag="WM481_000293"
     rRNA            2568..5459
                     /locus_tag="WM481_000293"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5556..5669
                     /gene="rrf"
                     /locus_tag="WM481_000294"
     rRNA            5556..5669
                     /gene="rrf"
                     /locus_tag="WM481_000294"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5959..6321
                     /locus_tag="WM481_000295"
     CDS             5959..6321
                     /locus_tag="WM481_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFA9079700.1"
                     /translation="MLIRHIVARCGLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNI
                     NAASQQELEALPGIGPAKAKAIAEYRAQNGAFKSVDDLIKVKGIGPAVLAKLKDQASV
                     GAPAPKGPAKPVLPAVKK"
     gene            complement(6726..7175)
                     /locus_tag="WM481_000296"
     CDS             complement(6726..7175)
                     /locus_tag="WM481_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HFA9079701.1"
                     /translation="MNTFFRQTAQAMTAKHIGRFPLSELDQVIDWQPIEQYLNRQKTR
                     YLRDRRGRPAHPLSSMFKAVLPGQWHSLSDPELEHSLITRIGFNLFCRFDGPGIPGCS
                     TLCRYRKFRYARAAYFGLLKVGAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            7400..8710
                     /locus_tag="WM481_000297"
     CDS             7400..8710
                     /locus_tag="WM481_000297"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFA9079702.1"
                     /translation="MSVTVEILENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVDREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEPTLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALIKAVELKVPVALVNEEAARLANEMKQNFVNQGMTDAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADTSRLQAPTSLAVESNVVDFVLGKAKVNKKALSFDEVMGAQA"
     gene            8806..9420
                     /gene="clpP"
                     /locus_tag="WM481_000298"
     CDS             8806..9420
                     /gene="clpP"
                     /locus_tag="WM481_000298"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFA9079703.1"
                     /translation="MSFDNHLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLRL"
     gene            complement(9474..10739)
                     /gene="ispG"
                     /locus_tag="WM481_000299"
                     /gene_synonym="gcpE"
     CDS             complement(9474..10739)
                     /gene="ispG"
                     /locus_tag="WM481_000299"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFA9079704.1"
                     /translation="MNTLQRRKTHQVLIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLVSSAPKPPEEVMKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVHDLIQVYRE
                     LGSRCVYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGNNIASEFL
                     AIVEEYVKTNYGKNSSKRNKGKVIPIQSL"
     gene            complement(10755..11516)
                     /gene="pilW"
                     /locus_tag="WM481_000300"
     CDS             complement(10755..11516)
                     /gene="pilW"
                     /locus_tag="WM481_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFA9079705.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSNPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNVQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(11519..12613)
                     /gene="rlmN"
                     /locus_tag="WM481_000301"
     CDS             complement(11519..12613)
                     /gene="rlmN"
                     /locus_tag="WM481_000301"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFA9079706.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGVNDKAQHAY
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRIFRDILQQAEFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(12757..13182)
                     /gene="ndk"
                     /locus_tag="WM481_000302"
     CDS             complement(12757..13182)
                     /gene="ndk"
                     /locus_tag="WM481_000302"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFA9079707.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPTEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(13241..14392)
                     /locus_tag="WM481_000303"
     CDS             complement(13241..14392)
                     /locus_tag="WM481_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HFA9079708.1"
                     /translation="MNNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHRRLKALKSESNPLKSVAAEITKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSGFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRTIWFDF
                     RALCFSPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(14922..15749)
                     /gene="fghA"
                     /locus_tag="WM481_000304"
     CDS             complement(14922..15749)
                     /gene="fghA"
                     /locus_tag="WM481_000304"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFA9079709.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPDNRENQPLG
                     VIYWLSGLTCTEQNFITKSSFRRYAAEHQVVVVAPDTGPRGEQVPNGAAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEEHFPTNGKRSIMGHSMDGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTKDFIETCRAANQPIDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(15758..16868)
                     /locus_tag="WM481_000305"
                     /pseudo
     CDS             complement(15758..16868)
                     /locus_tag="WM481_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
     gene            17018..17425
                     /locus_tag="WM481_000306"
     CDS             17018..17425
                     /locus_tag="WM481_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFA9079710.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLYLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(17639..17935)
                     /locus_tag="WM481_000307"
     CDS             complement(17639..17935)
                     /locus_tag="WM481_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFA9079711.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLA"
     gene            complement(17946..19631)
                     /gene="rpsA"
                     /locus_tag="WM481_000308"
     CDS             complement(17946..19631)
                     /gene="rpsA"
                     /locus_tag="WM481_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFA9079712.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QATRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(19784..20440)
                     /locus_tag="WM481_000309"
     CDS             complement(19784..20440)
                     /locus_tag="WM481_000309"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFA9079713.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSALAKKLPAVFSGNRILLDGEDVSDGIRTEAIGMGASAVAQWP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGFTFERILSDIETRDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(20673..22043)
                     /locus_tag="WM481_000310"
     CDS             complement(20673..22043)
                     /locus_tag="WM481_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9079714.1"
                     /translation="MSSPSNTNRQTWSSRLTYILTVAGATVGFGATWRFPYLVGENGG
                     GAYVFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPVAKIWKLVGRMGL
                     LGAFGIMAYYMVLGGWVISYIVNIIGGNLNISSPVDGVVTKGFFTEHIENSPWEIAFY
                     TLLFVAVNQWILVKGVIGGIEKTAKYLMPLLFLFLIAMVVRNVTLPGAMEGVAFYLKP
                     DFSKITAELFVFVLGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAVL
                     AGFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSHMWAGPVFAVIFFSLLLIAALTTSLT
                     IYEVLITTIQEKTKIRRTAAITIVLSVIFVFGNIPSILSYGPWKDVSVFGKNIFDAFD
                     YISGNILFMLTALGSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLI
                     FVSNLF"
     gene            complement(22517..23209)
                     /locus_tag="WM481_000311"
     CDS             complement(22517..23209)
                     /locus_tag="WM481_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFA9079715.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIRGGYVSINGTCMDDTDADIDSS
                     YVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVPLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(23277..25253)
                     /locus_tag="WM481_000312"
     CDS             complement(23277..25253)
                     /locus_tag="WM481_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9079716.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILD
                     LPPTKQYFQSGSLWDELRQGFRMGEVNPELVRRHESKFIASRSYFDRVVNRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKNKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLHTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVSIARIQPAAAQTADITVAPLPQETVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQVVPPNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAETSPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            25432..26097
                     /locus_tag="WM481_000313"
     CDS             25432..26097
                     /locus_tag="WM481_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFA9079717.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFNRYVASYRFSGMCAVQVGGQWL
                     SLSEDIVCVPRDMSMSAENMALADVFADMLLLPHTLECGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFCRWFDGVRLPEKRFCLPLPELKRQLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYWGGKALAAGAAR
                     VAD"
     gene            26150..26977
                     /gene="mutM"
                     /locus_tag="WM481_000314"
     CDS             26150..26977
                     /gene="mutM"
                     /locus_tag="WM481_000314"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFA9079718.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVILRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEERHPLLEKLGPEPLSEAFCTDYLYAGLKAQKRAV
                     KLALMDNTVVVGVGNIYANESLFRAGISPHRPANRLKKKECAVLVETVKAVLQRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCLRCGGLVVKETLGQRGTFYCTNCQK"
     gene            27065..27832
                     /locus_tag="WM481_000315"
     CDS             27065..27832
                     /locus_tag="WM481_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9079719.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPKSRNRAV
                     IALGKGALAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVAGDADVAV"
     gene            27935..28045
                     /locus_tag="WM481_000316"
                     /pseudo
     CDS             27935..28045
                     /locus_tag="WM481_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(28095..28406)
                     /locus_tag="WM481_000317"
     CDS             complement(28095..28406)
                     /locus_tag="WM481_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFA9079720.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFR
                     R"
     gene            28937..31216
                     /gene="nrdA"
                     /locus_tag="WM481_000318"
     CDS             28937..31216
                     /gene="nrdA"
                     /locus_tag="WM481_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HFA9079721.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYNHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEVRLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVSGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPDGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMDRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGNKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            31529..32662
                     /gene="nrdB"
                     /locus_tag="WM481_000319"
     CDS             31529..32662
                     /gene="nrdB"
                     /locus_tag="WM481_000319"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HFA9079722.1"
                     /translation="MSYSTFPKTKNDALKEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWVETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDPEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLSAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            32783..33073
                     /locus_tag="WM481_000320"
     CDS             32783..33073
                     /locus_tag="WM481_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HFA9079723.1"
                     /translation="MARIGTNKGLFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLAGEDEGLSDSLLK"
     gene            complement(33114..33392)
                     /gene="ftsB"
                     /locus_tag="WM481_000321"
     CDS             complement(33114..33392)
                     /gene="ftsB"
                     /locus_tag="WM481_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFA9079724.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(33560..34846)
                     /gene="eno"
                     /locus_tag="WM481_000322"
     CDS             complement(33560..34846)
                     /gene="eno"
                     /locus_tag="WM481_000322"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFA9079725.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKCNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGK"
     gene            complement(34893..35333)
                     /locus_tag="WM481_000323"
     CDS             complement(34893..35333)
                     /locus_tag="WM481_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFA9079726.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGTVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSVAGGSFGMWHGISSMTFMATALLSAVLSWRLSGKEAV"
     gene            complement(35348..36190)
                     /gene="kdsA"
                     /locus_tag="WM481_000324"
     CDS             complement(35348..36190)
                     /gene="kdsA"
                     /locus_tag="WM481_000324"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFA9079727.1"
                     /translation="MDIKINDITLGNNSPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLENFLIRIKALDDLIKSQPI
                     LTIE"
     gene            complement(36212..36595)
                     /locus_tag="WM481_000325"
     CDS             complement(36212..36595)
                     /locus_tag="WM481_000325"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFA9079728.1"
                     /translation="MFRTILGGKIHRATVTEADLNYVGSITVDQDLLDAAGICPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYIQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            complement(36757..37059)
                     /locus_tag="WM481_000326"
     CDS             complement(36757..37059)
                     /locus_tag="WM481_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2004 domain-containing protein"
                     /protein_id="HFA9079729.1"
                     /translation="MDAFAAFLSELEKADDAARAALTEYLKRDSRYIDFHTEDTQTSR
                     DAAKFVRTMQLIYISLWAKNPAFAVMDYMPANMESDEILAVKLHLDGSIFSIDWES"
     gene            complement(37285..40989)
                     /gene="mfd"
                     /locus_tag="WM481_000327"
     CDS             complement(37285..40989)
                     /gene="mfd"
                     /locus_tag="WM481_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFA9079730.1"
                     /translation="MTCPIPKPREKSRWFNLSQGSLPLALARYLPHKRLKAVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSETPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWNDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKAHSLPDLAVNRQSDEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSSSQQQTVPASEGEGKAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDGLKAAAVSTESSLYLVASDLHGQTRQQSAPSP
                     VGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHNRRKKHAAVSDGLLRDLAEINIG
                     DPVVHEEHGIGRYTGLVTMDLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSGQAHE
                     SVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQSGHKFEINESDYQAFADG
                     FGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTEVALRAAFVAVMGGKQVAV
                     LAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKATLEGMADGTVDIVIGTHK
                     LVQDDIKFKNLGLLIIDEEHRFGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGL
                     RDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRER
                     LETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIIN
                     RADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLA
                     MQDLEIRGAGEILGEGQSGEMMQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEI
                     KLHSPALLPEDYCPDIHERLVLYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIE
                     SHHLRLAAKELGIDAIDATSEAVTVTFGKHHCIDPTGIILLIQTDKKYRLAGADKLRF
                     AAEMENIEVRINTVKTVLKTLQGKRLPKGN"
     gene            41174..42601
                     /locus_tag="WM481_000328"
     CDS             41174..42601
                     /locus_tag="WM481_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9079731.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEGK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGGEAQVAQGLDMIFKGEGGGLGVTEPETSGAAIAREMQSCYEYTDEQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILDNLNRYVRNDIRFV
                     DYERREIQRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGIKLDKN
                     QTLLDRLQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     EEHEDTTAFLLNDIRKDILDCRYCG"
     gene            42749..43840
                     /gene="mltB"
                     /locus_tag="WM481_000329"
     CDS             42749..43840
                     /gene="mltB"
                     /locus_tag="WM481_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFA9079732.1"
                     /translation="MEKRKILPLAICLAALSACTAMEARTPRANEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGRAKFHGARRFYAENRAVIDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(43963..45969)
                     /locus_tag="WM481_000330"
     CDS             complement(43963..45969)
                     /locus_tag="WM481_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFA9079733.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFNPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGGIIALMALLKIRIGTLDLSPFLASLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLSKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIINHPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGFGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKGQGHPPSDKP"
BASE COUNT        10947 a        10872 c        12915 g        11431 t
ORIGIN      
        1 ttcaggcggc atattcacaa aggcgcatca gccggaggag aagagggggg ttgttggagg
       61 cggcgcagcg tttggcggag ataaaaaacc ttatccgaca gcgacatgac gaatttcccc
      121 aaaaaaaatc ccgctgaaag tattgactgt tttcccctgc gggcgtatag ttcggttctt
      181 cgccgctgcc gaagcggcgg acgaactgaa aagtatagca cggaatgtcg gaaatatcga
      241 gagatatctt agacaggcgg aagggatact ttataatttc gcaacgctct ttaacaaaac
      301 agattaccga taagtgtgag tgccttgggc ctcgcactgt ttgaaagaca gacgagatga
      361 tgttttgaac attgtcctgt cggtttcttt gaagcagacc agaagttaaa aagttagaga
      421 ttgaacataa gagtttgatc ctggctcaga ttgaacgctg gcggcatgct ttacacatgc
      481 aagtcggacg gcagcacagg gaagcttgct tctcgggtgg cgagtggcga acgggtgagt
      541 aacatatcgg aacgtaccgg gtagcggggg ataactgatc gaaagatcag ctaataccgc
      601 atacgtcttg agagggaaag caggggacct tcgggccttg cgctatccga gcggccgata
      661 tctgattagc tggttggcgg ggtaaaggcc caccaaggcg acgatcagta gcgggtctga
      721 gaggatgatc cgccacactg ggactgagac acggcccaga ctcctacggg aggcagcagt
      781 ggggaatttt ggacaatggg cgcaagcctg atccagccat gccgcgtgtc tgaagaaggc
      841 cttcgggttg taaaggactt ttgtcaggga agaaaaggcc gttgccaata tcggcggccg
      901 atgacggtac ctgaagaata agcaccggct aactacgtgc cagcagccgc ggtaatacgt
      961 agggtgcgag cgttaatcgg aattactggg cgtaaagcgg gcgcagacgg ttacttaagc
     1021 aggatgtgaa atccccgggc tcaacccggg aactgcgttc tgaactgggt gactcgagtg
     1081 tgtcagaggg aggtggaatt ccacgtgtag cagtgaaatg cgtagagatg tggaggaata
     1141 ccgatggcga aggcagcctc ctgggataac actgacgttc atgtccgaaa gcgtgggtag
     1201 caaacaggat tagataccct ggtagtccac gccctaaacg atgtcaatta gctgttgggc
     1261 aacttgattg cttggtagcg tagctaacgc gtgaaattga ccgcctgggg agtacggtcg
     1321 caagattaaa actcaaagga attgacgggg acccgcacaa gcggtggatg atgtggatta
     1381 attcgatgca acgcgaagaa ccttacctgg ttttgacatg tgcggaatcc tccggagacg
     1441 gaggagtgcc ttcgggagcc gtaacacagg tgctgcatgg ctgtcgtcag ctcgtgtcgt
     1501 gagatgttgg gttaagtccc gcaacgagcg caacccttgt cattagttgc catcattcgg
     1561 ttgggcactc taatgagact gccggtgaca agccggagga aggtggggat gacgtcaagt
     1621 cctcatggcc cttatgacca gggcttcaca cgtcatacaa tggtcggtac agagggtagc
     1681 caagccgcga ggcggagcca atctcacaaa accgatcgta gtccggattg cactctgcaa
     1741 ctcgagtgca tgaagtcgga atcgctagta atcgcaggtc agcatactgc ggtgaatacg
     1801 ttcccgggtc ttgtacacac cgcccgtcac accatgggag tgggggatac cagaagtagg
     1861 tagggtaacc gcaaggagcc cgcttaccac ggtatgcttc atgactgggg tgaagtcgta
     1921 acaaggtagc cgtaggggaa cctgcggctg gatcacctcc tttctagaga aagaaggggc
     1981 tttaggcatt cacacttatc ggtaaactga aaagatgcgg aagaagcttg agtgaaggca
     2041 aggttcgctt aagaagggaa accgggtttg tagctcagct ggttagagca cacgcttgat
     2101 aagcgtgggg tcggaggttc aagtcctccc agacccacca agaacggggg catagctcag
     2161 ttggtagagc acctgctttg caagcagggg gtcatcggtt cgatcccgtt tgcctccacc
     2221 aaaactttac aaatgaaagc aagtttgctg tttttagcag cttattttga tttgcgaagt
     2281 agaataacga cgcatcgatc tttaacaaat tggaaagccg aaatcaacaa acaaagacaa
     2341 tgagtttgtt ttgatttttt attctttgca aaggataaaa acctctcgca agagaaaaga
     2401 aaacaaacat agtatttggg tgatgattgt atcgacttaa tcctgaaaca caaaaggcag
     2461 gattaaggca caacaaagca gtaagcttta tcaaagtagg gatttcaagt ttgcttactt
     2521 agtcaacggg taggtaaacg aagtcaaaga agttcttgaa atgatagggt caagtgaata
     2581 agtgcatcag gcggatgcct tggcgatgat aggcgacgaa ggacgtgtaa gcctgcgaaa
     2641 agcgcggggg agctggcaat aaagctatga ttccgcgatg tccgaatggg gaaacccact
     2701 gcattctgtg cagtatccta agttgaatac ataggcttag agaagcgaac ccggagaact
     2761 gaaccatcta agtacccgga ggaaaagaaa tcaaccgaga ttccgcaagt agtggcgagc
     2821 gaacgcggag gagcctgtac gtaataactg tcgagataga agaacaagct gggaagcttg
     2881 accatagcgg gtgacagtcc cgtattcgaa atctcaacag cggtactaag cgtacgaaaa
     2941 gtagggcggg acacgtgaaa tcctgtctga atatgggggg accatcctcc aaggctaaat
     3001 actcatcatc gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag aaccccggga
     3061 ggggagtgaa acagaacctg aaacctgatg catacaaaca gtgggagcgc cctagtggtg
     3121 tgactgcgta ccttttgtat aatgggtcaa cgacttacat tcagtagcga gcttaaccgg
     3181 ataggggagg cgtagggaaa ccgagtctta atagggcgat gagttgctgg gtgtagaccc
     3241 gaaaccgagt gatctatcca tggccaggtt gaaggtgccg taacaggtac tggaggaccg
     3301 aacccacgca tgttgcaaaa tgcggggatg agctgtgggt aggggtgaaa ggctaaacaa
     3361 actcggagat agctggttct ccccgaaaac tatttaggta gtgcctcgag caagacactg
     3421 atgggggtaa agcactgtta tggctagggg gttattgcaa cttaccaacc catggcaaac
     3481 tcagaatacc atcaagtggt tcctcgggag acagacagcg ggtgctaacg tccgttgtca
     3541 agagggaaac aacccagacc gccggctaag gtcccaaatg atagattaag tggtaaacga
     3601 agtgggaagg cacagacagc caggatgttg gcttagaagc agccatcatt taaagaaagc
     3661 gtaatagctc actggtcgag tcgtcctgcg cggaagatgt aacggggctc aaatctataa
     3721 ccgaagctgc ggatgccggt ttaccggcat ggtaggggag cgttctgtag gctgatgaag
     3781 gtgcattgta aagtgtgctg gaggtatcag aagtgcgaat gttgacatga gtagcgataa
     3841 agcgggtgaa aagcccgctc gccgaaagcc caaggtttcc tacgcaacgt tcatcggcgt
     3901 agggtaagtc ggcccctaag gcgaggcaga aatgcgtagt cgatgggaaa caggttaata
     3961 ttcctgtact tgattcaaat gcgatgtggg gacggagaag gttaggttgg caagctgttg
     4021 gaatagcttg tttaagccgg taggtggaag acttaggcaa atccgggttt tcttaacacc
     4081 gagaagtgat gacgagtgtc tacggacacg aagcaaccga taccacgctt ccaggaaaag
     4141 ccactaagct tcagtttgaa tcgaaccgta ccgcaaaccg acacaggtgg gcaggatgag
     4201 aattctaagg cgcttgagag aactcgggag aaggaactcg gcaaattgat accgtaactt
     4261 cgggagaagg tatgccctct aaggttaagg acttgctccg taagccccgg agggtcgcag
     4321 agaataggtg gctgcgactg tttattaaaa acacagcact ctgccaacac gaaagtggac
     4381 gtatagggtg tgacgcctgc ccggtgccgg aaggttaatt gaagatgtgc aagcatcgga
     4441 tcgaagcccc ggtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg
     4501 tcgggtaagt tccgacccgc acgaatggcg taacgatggc cacactgtct cctcccgaga
     4561 ctcagcgaag ttgaagtggt tgtgaagatg caatctaccc gctgctagac ggaaagaccc
     4621 cgtgaacctt tactgtagct ttgcattgga ctttgaagtc acttgtgtag gataggtggg
     4681 aggcttggaa gcagagacgc cagtctctgt ggagtcgtcc ttgaaatacc accctggtgt
     4741 ctttgaggtt ctaacccaga cccgtcatcc gggtcgggga ccgtgcatgg taggcagttt
     4801 gactggggcg gtctcctccc aaagcgtaac ggaggagttc gaaggttacc taggtccggt
     4861 cggaaatcgg actgatagtg caatggcaaa aggtagctta actgcgagac cgacaagtcg
     4921 ggcaggtgcg aaagcaggac atagtgatcc ggtggttctg tatggaaggg ccatcgctca
     4981 acggataaaa ggtactccgg ggataacagg ctgattccgc ccaagagttc atatcgacgg
     5041 cggagtttgg cacctcgatg tcggctcatc acatcctggg gctgtagtcg gtcccaaggg
     5101 tatggctgtt cgccatttaa agtggtacgt gagctgggtt taaaacgtcg tgagacagtt
     5161 tggtccctat ctgcagtggg cgttggaagt ttgacggggg ctgctcctag tacgagagga
     5221 ccggagtgga cgaacctctg gtgtaccggt tgtaacgcca gttgcatagc cgggtagcta
     5281 agttcggaag agataagcgc tgaaagcatc taagcgcgaa actcgcctga agatgagact
     5341 tcccttgcgg tttaaccgca ctaaagggtc gttcgagacc aggacgttga taggtggggt
     5401 gtggaagcgc ggtaacgcgt gaagctaacc catactaatt gcccgtgagg cttgactcta
     5461 tcatttgaag gacttcaaga gataaaagct tactgactga ttcagtcatt accgaatata
     5521 ttgattaagg ctttaccgat ttgcaacagt ttaagtttgg cggccatagc gagttggtcc
     5581 cacgccttcc catcccgaac aggaccgtga aacgactcag cgccgatgat agtgtggttc
     5641 ttccatgcga aagtaggtca ctgccaaaca ccctttcaga aaacccccgg atatccgggg
     5701 gtttttgctt tgcccggaaa aaatgtcggg gatggcggga cggcatctgt acggtgtccg
     5761 gtcgggtttg cggaggaacg gcttgaaact ttgggatatt cattttagaa tgacccgttt
     5821 tatagcggat taacaaaaat caggacaagg cggcgggccg caggcagtac ggatagtacg
     5881 gaaccggttc gcccggtgct tcatcacctt agggaaccgt tccctttgag ccggggcggg
     5941 gcaacgccgt accggttttt gttaatccgc catatcgtcg caagatgcgg tttgttgttt
     6001 gcaaccctta aaggaaaaac catgaaaaaa atgtttgtat tgttctgtat gctgttctcc
     6061 tgcgccttct cccttgcggc ggtaaacatc aatgcggctt cgcagcagga gctggaggcg
     6121 ctgccgggca taggcccggc gaaggcgaag gccattgcgg aataccgcgc gcaaaacggc
     6181 gcgttcaagt ctgtggacga tttgatcaag gtgaagggca tcggtccggc ggtgctggcg
     6241 aagctgaaag accaggcttc cgtcggcgcg cccgcaccaa aaggccccgc caaaccggtg
     6301 ctgcctgcgg ttaaaaaata ggggaacctg taaaggaagg ggcatcggct gccgccggcg
     6361 cttttttgtt ctttgtttgg aagggaaatg gctggagaaa gacctgcgct atatcgggga
     6421 atgaatcgat gcaaaggaaa tgccgtctga acatgcgttc gggcagcgtt tttataacaa
     6481 aaacacttaa tggcggttgg ttttatacct atctaagttt ttgtgtcgtg catatctgaa
     6541 aagtgtattc cgtttcagtt tgtaagctgt ggcattctga gacctttgca aaaaagccct
     6601 tccttcgaca gccgaaaccc aaacacaggt tttcagctgt tttcgcccca aatacctcct
     6661 aattttaccc gaatatcccc ttaatcctcc ccggataccc gataatcagg catccgggcc
     6721 gccttttagg cggcagcggg cgcacttagc ctgttggcgg ctttcaacag gttcaaacac
     6781 atcgccttca ggtggctttg cgcacccact ttgagcagcc cgaaataggc tgcccgcgca
     6841 tagcggaatt tacggtagcg gcataaggtg ctgcaaccgg ggatgcccgg tccgtcaaaa
     6901 cggcaaaaca ggttgaaacc gatgcgggtg atgaggctgt gttcgagttc gggatcggag
     6961 aggctgtgcc attgtccggg caggacggct ttgaacatgg acgacagggg atgggcggga
     7021 cggccgcggc ggtctcggag gtaacgggtt ttttgacggt tcaggtattg ttcgatcggc
     7081 tgccaatcaa tcacctggtc caactccgat agcgggaagc ggccgatgtg tttggcagtc
     7141 atggcttggg cggtttgccg gaagaaggtg ttcatgggaa atcccctaaa tgccttggtg
     7201 ggaatttagg ggattttagg ggaattttgc aaaggtctcg gcatattatg tttcaggcga
     7261 aacattgtga tatacttgcc agctaaaatt tcccctttgc ggcaatgcgg ttcaaatatc
     7321 gtaccgttgc gctgtttgct tccccatgca gggaagaagg tttatcattt tatcaacaca
     7381 acaaatttaa gggcttatga tgagcgtaac tgttgaaatt ttagaaaatc tggaacgcaa
     7441 agtagtgttg tccctgcctt ggtccgaaat caacgcagaa accgataaaa aactgaaaca
     7501 aacccaacgc cgtgcaaaaa tcgacggttt ccgtccgggt aaagcacctt taaaaatgat
     7561 tgcccaaatg tacggtgcga gcgcgcaaaa cgacgtgatc aacgagctgg tgcaacgccg
     7621 cttctacgat gttgccgttg cccaagagtt gaaagtggca ggctatccac gttttgaagg
     7681 cgttgaagaa caagacgata aagagtcttt caaagttgcc gccatttttg aagtgttccc
     7741 cgaagtcgtt atcggcgatt tgtctgcaca agaggtcgaa aaagtaaccg cttccgtcgg
     7801 cgatgccgaa gtcgaccaaa ctgtagaaat cctgcgtaaa caacgtaccc gcttcaacca
     7861 tgttgaccgc gaagcccgaa acggcgaccg cgtcatcatc gactttgaag gcaaaatcga
     7921 cggcgaacct tttgccggcg gcgcatccaa aaactacgcc ttcgtattgg gcgcaggtca
     7981 aatgctgcct gaatttgaag ccggcgtagt cggcatgaaa gcgggcgaaa gtaaagacgt
     8041 taccgtcaac ttccctgaag aataccacgg caaagatgtt gccggtaaaa ctgccgtgtt
     8101 caccattacg ctgaacaatg tttccgagcc cactctgcct gaagtcgatg cagattttgc
     8161 aaaagccttg ggtattgcgg atggcgatgt tgccaaaatg cgtgaagaag tgaagaaaaa
     8221 cgtaagccgc gaagtggaac gccgcgtgaa cgaacaaacc aaagaatccg taatgaacgc
     8281 gctgattaaa gccgtagagt tgaaagttcc ggttgctttg gtcaatgaag aagccgcccg
     8341 cctggcaaac gaaatgaaac aaaacttcgt taaccaaggt atgaccgatg ccgcgaactt
     8401 ggatttgcct ttggatatgt tcaaagaaca agccgaacgt cgcgtatctt tgggtctgat
     8461 tttggccaaa ctggttgacg aaaacaaact ggaaccgact gaagagcaaa tcaaagccgt
     8521 tgtcgccaac ttcgcagaaa gctacgaaga tcctcaagaa gtgattgact ggtactacgc
     8581 agatacttcc cgcctgcaag ccccgacttc tttggcagta gaaagcaacg ttgttgattt
     8641 cgttttgggc aaagccaaag taaacaaaaa agctttgtct tttgacgaag tgatgggcgc
     8701 gcaagcctga ttgttctgaa aatgctgtct gaaggcagtt tttgaaagca ccgaagccgt
     8761 ttttaaagcc cgctttggtg cttttccatc atgaaaggag acgaaatgtc ttttgataac
     8821 catcttgtcc ctaccgttat cgagcagagc ggtcgcggtg agcgtgcatt cgatatctat
     8881 tcccggcttt tgaaagagcg catcgtattt ctggttggcc cggtaaccga tgagtctgct
     8941 aatctggtgg tcgcccaact gttgtttttg gaaagtgaga atccggataa ggatattttc
     9001 ttctacatca attcccccgg cggctcggta acggccggta tgtcgattta cgacacgatg
     9061 aatttcatca agcccgatgt atcgactttg tgcttggggc aggcggcaag tatgggcgcg
     9121 ttcttattgt cggcaggcga gaaaggcaaa cgtttcgccc tgcccaacag ccggattatg
     9181 attcaccagc ctttaatcag cggcggcttg ggcggtcagg catccgacat tgaaatccac
     9241 gcacgcgagt tgttgaaaat caaagaaaaa ctcaaccgcc tgatggcgaa acattgcggc
     9301 cgcgatttgg cagatttgga gcgcgacacc gaccgtgata atttcatgtc tgccgaagaa
     9361 gcaaaagaat atggtttgat cgaccaagtt ttggaaaacc gcgcttcttt gcggctttaa
     9421 taagaaatct gagagaaaca aatgccgtct gaaacgttca gacggcattt gttttataga
     9481 gactgtatcg ggatgacttt tcctttattg cgtttagaac tgtttttgcc ataattggtt
     9541 ttaacatatt cctctacaat agctaggaat tcgcttgcaa tattattacc tttcagtgtg
     9601 actttgcgct ccccatctac ataaacaggt gcgacgggtg tttcccccgt accgggaagg
     9661 ctgattccga tgtcggccaa tttgctttct ccggggccat tgacaacgca gcccattacg
     9721 gcaacgttca gggattcaac cccgggataa agggtacgcc atatagacat tttttggcgc
     9781 aggtaatttt gaacatcttg tgccagctct tgaaatacgg tactggtggt acgcccgcat
     9841 cccgggcagg cggtaaccat cggcgtaaac gagcgcaaac ccatagtctg taaaatctct
     9901 tgcccgacga cgacctcctg agtacgcggg ctgcccggtt ccggagtgag tgaaatgcgg
     9961 atggtgtcgc caattccttc ttgaagcaaa acagataatg ccgccgttga tgcgacaatg
    10021 cctttgctgc ccataccggc ttcggtcaaa cccaaatgca gcggatagac gcaacggctg
    10081 cccagttcgc ggtaaacctg aatcaaatca tgaaccgcgc tgactttgca cgacaggatg
    10141 attttgtctt cgggcagtcc caatagaacg gctttttcgg cagattccaa agcggagaca
    10201 atcagtgctt ccttcatcac ttcttcgggc ggtttcggcg cggaagaaac gaggttggca
    10261 tccatcatac gtttggcgag gctctgatcc aaagaacccc agtttacgcc gatacggacg
    10321 gctttatcgt tttcagcagc agtccgaatc ataaaggcaa atttttcatc gccttttacg
    10381 cctttgccga cattgccggg attgatgcgg tatttggaca atgctttgcc gcattcggga
    10441 aattccgcca acaggcgttc gccgttaaaa tggaaatcgc caatcagtgg tgtggcatag
    10501 cccatatcgt ccaagcggcg gcggatttcg gcaactttgg acgcggcttc ggggctgttg
    10561 acggtaatcc gcaccatttc cgagccggca tcgctcaatt ccttaatctg caatgcggtg
    10621 gcttttgcat cggcagtgtc ggtgttggtc atagattgga taacgacggg tgcttctgaa
    10681 ccgacggtga tatgatcgat gaggacttga tgcgtcttgc ggcgttggag tgtgttcata
    10741 tggtttgaat ctgtttattg accggtgagg acggtttgca attcttccga gtaggggaaa
    10801 tttgcctgca attgtgcttc atattcgtat gccgcctgca cgttgccgag ggctttggca
    10861 attttccagc ctagcagcaa atcatcggcc tgaaggactt ctaccctgct ttggtatttt
    10921 ttaaagtagt aatcggcatc gcccaactgc ccggccagca ttttggtgcg cgccagttct
    10981 ttaaatgcgg gtgggaactg cggctgggcg gcgagggaac gtttcaaata ggcttccgcc
    11041 aatccgaatt gcccctgttt tgcgctgcat atacctttat tcaggttggc aatataaggg
    11101 gtcgggtagg tggggtcggc cagggctttg tcgaaatatg ccatagattc ggcagggcgg
    11161 ttgagcctgc cgcacaggaa ccagccgtag ttgttgttga tttcggcact gtcgggtttg
    11221 atggagaggg cttgccggaa actttcctgc gccttgtcgt taactttcag gtattgatag
    11281 atttcggcac ggaccagcca ggcaagctcg tttttagggt tcgatttcaa ggcatcttca
    11341 atacttgccg ttgcctgacg gtagtcctga ccgcgcatat attccatcgc caactgggtt
    11401 ttgatattgg aaacctgatt ggctttttct gcccgcgagg ggcggtagga agtgctgcac
    11461 gcgcccaagg caagaacaag taataaagag attcgtttgg atggcttaaa aggcataatt
    11521 acccctgttg tccgattaaa atctgctgcc atttttgttg gcggcgcgtt ttatcctgaa
    11581 cctgacctgc caactgtccg caggcggcat cgatatcgtc gccacgagtt tttcgaacgg
    11641 taacgacaaa ttcggcttgt tgcaggatat ctctgaaaat acggatgttt tcgttacttg
    11701 aacgttcata tcccgaatta gggaaaggat taaacgggat taaattgaat ttgcagggaa
    11761 catctttgac cagttcaata agttcgtatg catgttgtgc cttatcgttg acaccgtcca
    11821 gcatcacata ctcgaaagtg atgaaatccc tgggcgcttt gaccagatag cgttggcatg
    11881 cggccatcaa ttctttcaag ggatattttt tgttcaacgg tacgatttgg ttgcggactt
    11941 cgtcattgga agcgtggagg gaaaccgcca aggccaccgg catgacatcg cgcaacctgt
    12001 ccatttgggg aaccataccc gaagtggaaa cggttacgcg gcggcggctc aaaccgtagc
    12061 cgtggtcgtc cagcatgatg ctcaaggcgg taacgacatt gtcgaagttc gccatcggct
    12121 cgcccatgcc catcatgacg acgttggaaa tcacacgctc gtttttcggt gtaacgccca
    12181 tcgctttgtt tgcccaccac aactgtccga tgatttcggc agcggttaaa ttgcggttga
    12241 agccctgccg gccggtcgaa caaaatgtac attccaaagc gcagccgact tgcgaggaaa
    12301 tgcagagcgt gccgcgatcc gattcgggga tgaagacggt ttccacgccg ttccccgtac
    12361 cgacatccaa aagccatttt cgagtgccgt ctgaagattc ttgggacatc atcagcttgg
    12421 gaatttcgat gcttgcctgt tcgttcagtt tatggcgcaa cgattttgcc aaatcggtca
    12481 tttcgtcaaa attttgcgcg ccggattggt gcatccaacg cataacctgt ttggcgcgga
    12541 atggtttttc acccatatcg gcaaaatgtc gggttagccc ttgaaggtcg tagttgagca
    12601 gattggtttt catgtatttg tgtttttctt aaattaaggc ttcaaataaa aaattgcagg
    12661 gcaggcaagt ttgatttgaa gtcttttgca acagagtttt cagacggcat ggatatattt
    12721 tatgccgtct gaataggaat tgggcggtgt attgtatcaa cgggggcaga tttcggtttg
    12781 gctgaaaaag taggcaattt ccaaagcggc attttccaca ctgtcggaac cgtgtacggc
    12841 gttaatgctg accgaagtgg caaagtccgc gcgtatcgtg ccttctgcgg cttcggtggg
    12901 attggtcgcc cccatcagtt cgcggttttt cagaacggcg ttttcgcctt ccagtacttg
    12961 aatcataacc ggaccgccgg tcataaattc aaccaatccg gcatagaagg ggcggtcttt
    13021 atgaaccgca taaaattctt gggcttcttt gagggtaagc tgcttcattt tggcggcaac
    13081 gattttcaga ccgttctcct caaagcggct gtatattttg ccgataacgt ttttgccgac
    13141 ggcatcgggt ttgatgatgg atatggtacg ttcaatcgcc atgctatatc cttattttta
    13201 cttaagaaga atcaaatcgg tattctacca aaaaaataac ttatccgcct gaagccttgg
    13261 gagataaggt caggtgcggc tgttccaaat aaacttcgga ctgcatttcg accatccggc
    13321 tggcggtgcg ggtaaattct tcggcaaaat cgccttccgt gtagatatgg ttgacatcta
    13381 ccgcgccggt ggcacacagt ttgacccgga aatcgtagag tacgtcaatc agccaagtca
    13441 gccgtcgcgc ctccgccttt tcttgcggtg agagttgttc caaacctgaa ataaaaacca
    13501 tttcataatg ttcggccaaa tacagatagt cggactgtga gcggggactg aagcacagtg
    13561 cgcggaaatc aaaccatatg gtacgaccgg actcggcttt gtggggaatt tcccggccgt
    13621 ggatggtgct gatgccgggg ttcaaatcgg taatgcctgt catttctttg aacagttttg
    13681 ccagttttgc ctcattttct tcattggcag gcgtaaagaa aatctcggcg gggcggaggg
    13741 tacgcagccg gtagtcttcc cctccgtcaa cgtttaagac ggtcagactg gactcgatga
    13801 gcgcgattgt gggaagaaaa ccgctccggt tttgaccttg cgggtagagt tcggaaggcg
    13861 cgtagtttga agtcgccacc aaaacaacgc cctcgttaag caggttttcc agcagacggc
    13921 ctaaaatcat tgcatccgca atatcgctga catgaaattc gtcaaaacac aatactcggg
    13981 tttctttggt aatctcggcg gcaacggatt tcaacgggtt gctttcgctt ttcagggctt
    14041 tcagccgccg gtggatttcc gccataaagg catgaaagtg gacgcggcgt ttgcggcggt
    14101 aagggaggca gccgaaaaaa gcgtccatca gaaagctctt gccgcgtccg actccgccat
    14161 agaaataaag ccctttggga acttgcgggg aacgcaaact cctgcctaaa aaacggtttc
    14221 ttttgcgttt gaacatcatc aattcggtcc aaagccggtc aaggtgttcg atggcggccg
    14281 cctgtgcgtc gtcgcggatg aagttgggca gttgtgaggc agcctgatac caggtcagcg
    14341 ggctgtggtt ttcaaacggc ggggctttaa aaagttggtc tctattattc atatttaacc
    14401 ttgttatact tcttgcggct aagaaagaaa atgcttaccg ctggtattat aaatgatttc
    14461 cttcaaactt ggcgtttccc gtggaaaaga ataatgacgc gcacaaaggg ggtataagca
    14521 aaggcggcgt tttaatatgg tgcagatatg agccaaaatg ccttttattt caggctgcgt
    14581 tttaatattg aaggaggaca aatgaaacag atggtgaatc gcgaagctgt tacgccaccg
    14641 tcagcttgga gggggcgaaa acacaacagg aacaggttgc ttatgcaaat tcggcaacga
    14701 ataatacgaa gcccgaaagt gaagtaggaa atacgggcga ttttaatgtt tgatcaatca
    14761 gggtttaagc ttaaaaatca tactaatcaa atcaagggca gggtttggta atccgtaggg
    14821 ccggctcctt ctggttcagg cggcatacgt ttatgccggc acttcttacc gccctcgtgc
    14881 cggctattcc aaactgcttt gaaaactgac cgctctttga cttacttcaa aaatgcggcg
    14941 tgataagcga tatgctcacc aataaaactg gcgatgaagt aatagctgtg atcgtatcct
    15001 ttatggaaac gcacatcgat tggctgattt gccgcacgac aggtttcgat aaaatctttg
    15061 gtacgcaatt gtgtcggcaa aaactcatct tccaagccct gatcgatacg cataccttgc
    15121 actttatagc cttgttgaat gagtgagtta gcatcatatt gctgccattt ttcacggtct
    15181 ttccctaaat aagcagtaaa ggctttttct ccccacggta cgaggcttgg cgataaaata
    15241 ggcgaaaagg cagaaacact ttgataatgt tcccgattcc gtagtgccaa taccaatgcg
    15301 ccgtgtccgt ccatcgaatg ccccataatg gaacgtttgc cgttggtagg aaaatgctcc
    15361 tcaatcagac ggggcaactc gttcaaaatg taatcataca tttgataatt cgccgcccaa
    15421 ggctgttcgg tcgcattcaa ataaaagcct gccccctgtc ctaaatcata agcggcaccg
    15481 ttcggcactt gctctccgcg agggccggta tcgggggcga ccacaaccac ttgatgttct
    15541 gccgcataac gccggaagct cgatttggta atgaaatttt gttccgtaca cgtcaagccg
    15601 gaaagccaat aaatcacacc aagcggttga ttttcccgat tatccggcaa atagacggca
    15661 aatttcattt cgcattgaag cgtttgggca tgatgcgccc aaacttgttg cgaaccacca
    15721 aaaatttgat gttgttcaat cagtttcatc gtggacctta gtaatgaata acagcgcgga
    15781 tcgatttacc ttcgtgcatt aactcaaagg cttcattgat ttgatcaagg gtcattgcat
    15841 gggtcacaaa tggttctaat tggatatcgc ctttcattga atcctccacc attttcggaa
    15901 gctcggagcg gcctttaaca ccaccaaatg ccgagccttt ccaaacacgg cctgttaccg
    15961 attggaacgg acgcgttgaa atttcttgtc ctgcatccgc tacgccgatg atgatggatt
    16021 gtccccaacc tcggtgtgca ctttctaatg cctgacgcat tacgtttaca ttgccgatac
    16081 attcaaaggt gtggtcaatg ccccatttgt tgatgtctaa caacacatct ttgattggtt
    16141 tatcgtaatc tttcgggctc aaacagtcag ttgcaccaac actgttttgc caactcgaat
    16201 tttgcaggat tggtatcaat ggcgataatg cgaccggctt tggcttgacg cgcaccttgc
    16261 accacagcca aaccaatcgc tcccaagcca aacacggcaa cagaatcgcc ttcttgtact
    16321 tttgccgtgt tatgtaccgc accaatacca gtggtaacgc cgcagccgag caaacatact
    16381 tgttcgtggt tggctttcgg gttgattttc gccagtgaaa cttcggcaac aacagaatat
    16441 tcactgaaag tcgaacagcc catatagtga taaatcggct gaccttgata agaaaaacgc
    16501 gtcgtgccgt ctggcattaa gcctttacct tgtgtatcac gcactgagac gcataaattg
    16561 gttttacccg agaggcaaaa ctcacatttg ccacattctg cggtgtaaag cggaatgacg
    16621 tgattacccg gttttacgct tgacacaccc tcgcccacag caacgaccac acccgcacct
    16681 tcgtgtccaa gcaccacagg gaatacgcct tcaggatcgc ttcctgataa ggtaaatgca
    16741 tcagtatggc acacgccagt gtgggtgtta cgaattaaca cctcgtcttt acgcggcatt
    16801 tccacgtcga tttccacaat ttgtaagggt tggttagggg cgaatgccac cgccgcacga
    16861 gatttgatgg ttgaatcggt ttgtttcatt tccattgtct ttcctcttaa ttcattaaca
    16921 aaatagcctt gcctgagcaa catagcaaca gtttacattt tagagttcac tctaaggcaa
    16981 gtgttttttt accgtttttt gtttagaggg agggaaaatg acttatacca ctgccaaagc
    17041 tgccgaaaaa ataggcatct ccgcccacac tttacgcttt tacgacaaag aaggcttgtt
    17101 gcctaatatc ggacgtgatg aatacggtaa ccgttgtttt accgataacg atttgcaatg
    17161 gttgtactta ttgcaatgct tgaaaaatac gggaatgagc ttaaaagaca tcaaacgctt
    17221 tgcggaatgt accgtcattg gcgacgatac cattgaagaa cgcctttcct tgtttgaaaa
    17281 tcaaatagaa aatgtgaagt gtcaaattgc cgaattaaaa cgttatttag atttgcttga
    17341 atacaaattg gcgttttacc aaaaagcgaa agcattaggc tcggtaaaag ctgtaaattt
    17401 gccgcaaatt cctgaaacgg cttagtttta gtgcaataag caggattttg tcctgttcgt
    17461 aatagaaatc aacaatttgt tgtttaaaaa gcggattgta agcaatggta aaaaatccac
    17521 gctctaatcc gttgcttgct tggcttaagg tattgaagca gcggatgaag ttaaaccaaa
    17581 gaaaaaacgc cgcgtaccag cggcgttttg atgctaaggt ttaattggca ttttcttttc
    17641 aagccaagtc gacccgctcg cgcaattctt taccaggctt gaagtggggt acatgttttt
    17701 caggtacttc cacgcgctcg ccggttttgg gattgcgacc gatgcgggca ggacgatggt
    17761 tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg acctcgggca agcgatcggg
    17821 tcatggtgtc aaccaagact tttacgctgt actctacgtc ttttgccaga agatgcgtgc
    17881 cgtttttggc ggcaaatact tctgccaagc gaaccattaa ctcagacttt gtcatgtctg
    17941 caaccttatt cttgttcgcc ggagagtttg gctttcagca ggtcgcccaa gctggtggta
    18001 ccggcattcg cattggcggc ggcattgacg gagttcagtg cttcgcggct ttctttggca
    18061 tctttggctt taacggaaag tttgatgctg cggtttttgc ggtcaacggt aacgatgacg
    18121 gcttcaactt cgtcgccttc tttcagtttg gtggtcaagt cttcaacgcg gtcggctgca
    18181 aattcggaag caggcaggta gccttctact tcgtcagaca gggcgataac agcgcctttg
    18241 gcatcaacag atttcacgga acctttaacc aaagaacctt tgtcgttcac gctgatgaag
    18301 ttgccgaaag gatcgccttc cagttgtttg atacccaagg agatgcgttc tttttccaca
    18361 tcgattgcca atacgacggc ttcgacttct tctccttttt tgtatttgcg tacggcttct
    18421 tcgccggatt cggtccaaga caggtcggac aggtgaacca gaccgtcgat gccgccgggc
    18481 aggccgacga atacgccgaa atcggtaatg gatttaaccg caccggagat tttgtctcct
    18541 ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt ggcattgttt catacccaaa
    18601 gagatacggc ggcggccttc gtcgatttcc aaaatcatga cttcgacttc gtcacccagt
    18661 tgtacgactt tgctcgggtg tacgtttttg ttggtccagt ccatttcgga gacgtgtacc
    18721 aaaccttcga tgccttgttc gatttcgacg aatgcgccgt agtcggtcag gttggatact
    18781 ttgccgaaca ggcgggtggc ttgcggataa cggcgggtca gaccgctcca aggatcttcg
    18841 cccagttgtt tcatacccaa ggaaacacgt tgtttttctt ggtcgaattt caatactttg
    18901 gcttcaactt cctgaccgac ttccaagact tcgctcgggt gtttcacgcg acgccatgcc
    18961 aaatcggtga tgtgcagcag gccgtcgatg ccgcccaggt caacgaatgc gccgtagtcg
    19021 gtgatatttt tgacgatgcc tttgatgacg gagccttctt gcaggttttc cagcagggct
    19081 ttgcgttctt cacccaaagt ggcttccaaa acggcgcggc gggaaacaac gacgttgttg
    19141 cgttttttgt ccagtttgat cactttgaat tcgatctctt tgccttcaaa atgggaagtg
    19201 tctttaacgg gacgtacgtc gaccaaagaa cccggcagga atgcgcggat gctgctgatc
    19261 ataacggtca ggccgccttt gactttaccg ttgatgatgc cggacaggat gttgccgttt
    19321 tccatggctt cttccaaagc gatccaatcg gctgcgcgtt tggctttttc gcgggacagt
    19381 ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa cggtaacgaa gtcgccgact
    19441 ttaacttcaa tttcgccttg agcgtttttg aattcagcta catcgatcag ggattctgat
    19501 ttcagacctg cgtttacggt aacgaagttt tggtcgattg ccactacttc agcggtaatc
    19561 acctcacccg ggttcatttc ttgcagggta aagctttctt ccaacagctg agcaaaattt
    19621 tccatagaca tatataactc ttttcggtac accgccaagg ggtgcgggtt gggttggtgg
    19681 gtgtctgcag tccttggcac ggcaggcggg taaataaggt ttcagacggc acatggaaat
    19741 tcatgctgtc tgaagcgcaa tgcgggcgat tatacctgaa aatttaaact ttacgatacc
    19801 aatcaagcac tttttttaca gtttcttcta tagttaggcg gctcgtatcc aaaagcaggg
    19861 catcgggctg ttgtttcagg ggggcaacct tgcggtttcg gtctgcttcg tctctggtct
    19921 cgatgtcgga caggatgcgc tcgaatgtga aaccttcgca gggaatgccg atttgtttgg
    19981 cgcggcgttc ggcacggatt ttagattcgg ctgtcagaaa aattttgagt tcggcttggg
    20041 ggaagacgac cgatccggtg tcccgtccgt cggcaaccag tcctttttcg gtcagaaaat
    20101 cgcgttggcg ttgcagcagt gcggcgcgga ctttgggcca ttgcgcaact gcggatgcgc
    20161 ccataccgat ggcttctgtc cggatgccgt ctgaaacgtc ttcgccatca agtaggatgc
    20221 ggttgcctga aaatacggcg ggcagttttt tggcaagtgc ggaaacgttt tcttcatcgt
    20281 gccattccac gccttgtttt tgtgcatata gggcggtcag gcggtagagc gcgccggtat
    20341 cgagataatc gtatcccaat gcggcggcaa cgcgggcggc gaccgtgcct ttgcccgatg
    20401 cgcccggacc gtcgatggcg atgacttttt gtctgttcat aagggggatt cctgatggtt
    20461 tggggtatgg gttttgccgt ctgaagggtg tgtttcccgt tggggcggat tctacctgtt
    20521 ttaaaggacg attgtctaag cagacggaac gccgcctgcc cggaaagcgt ccgacagaag
    20581 ccggcaagcc ggttcggatt ccggcgtggc gggcatcaat gcaatgccgt ctgaaagagg
    20641 tatgaccttc agacggcatt gattgctgcg gattagaaca ggttgctgac gaaaatcagc
    20701 agaatcacca gcggcacaag gtatttcaca taggcaaacc aaatattgac cgtcgtatgg
    20761 ttgcctttat aaagcaattc ttccttcgct tcgtccttca tcacaaaacc gacaaacaat
    20821 gcggaaccga gcgcggtcag cataaacaag atgttgccgc tgatgtagtc gaaggcatcg
    20881 aaaatatttt tgccgaacac ggaaacgtct ttccacgggc catagctcaa aatagacggg
    20941 atgttgccga aaacgaagat gacggacaat acaatcgtga ttgcggcggt acggcggatt
    21001 ttggtttttt cctgaatggt cgtaatcaac acttcataaa tcgtcagcga agttgtcaac
    21061 gcggcaatca ggagcagaga gaagaaaatg acggcgaaca caggtcctgc ccacatatgt
    21121 gagaacacaa tcggtaaact ttggaacacc aaagtcgggc cggaattggg cgcgacgccg
    21181 aagctgaaga gcgacgggaa aatcataaag cccgcaagta cagcgatgat ggtattggta
    21241 attgccgtga taactgccgt ctgaaccaga ttttcgtttt tatccagata gctggataag
    21301 gtaatcatca cgccgaaacc caggctcagg gcaaaaaata cctgtcccaa aacgaagacg
    21361 aacagttcgg cggtaatctt gctgaaatca ggtttcagat agaaagcaac cccttccatt
    21421 gcgcccggaa gggtaacgtt gcggacgacc atcgcgatta ggaacaaaaa cagcagaggc
    21481 atcaggtatt tcgccgtttt ttcaatgccg ccgataacgc ctttgaccaa aatccattgg
    21541 ttcacggcga caaaaagcag cgtataaaac gcaatttccc aagggctgtt ttcaatgtgt
    21601 tcggtaaaga agccttttgt aaccacaccg tcgacggggc tggaaatatt caaatttcct
    21661 ccaataatat taacgatata gctgattacc cagccaccga gtaccatata ataagccatg
    21721 atgccgaacg cgccgagcag ccccatccgg ccgaccagtt tccaaatttt ggcaacaggt
    21781 ttgccgttca tcgggccgcc gaacgcatcc agcgcgttca cgcctttgcg ccgtccgatg
    21841 acgttttcca ccaaaatcat cgggatgccg ataaccagca tcgcgataca gaataaaaac
    21901 acatacgcgc cgccgccgtt ttcaccgacc aaatacggga aacgccacgt cgcgccgaaa
    21961 ccgacagtcg cgccggcaac ggtaaggata taagtcaaac ggctggacca ggtttgacgg
    22021 ttggtatttg aaggggaaga catattgaaa ccgtgtccga ttgagataaa gcggaaattt
    22081 tacacgtgat tcttaacaga aattgactga atgtttttca actgcctagt gattttccat
    22141 gtaaaatgca tatatttcag tgaatatttc tgatgagtat aaaccgatga ggaagaatcg
    22201 gagtttatga acagattaat ctgttatttt tctacatcgg tgtacggaag tgaaattttg
    22261 ttaatcatat taatgatagc atttcaatga tgatgaaaga gagggaattt gaaatagaaa
    22321 accatctata caagggggga agtatagatg gcaaaacaca ttacggggaa aacgtcttgc
    22381 tcataatcct gcttgaacag gcgttactta gacaagcgga ttatattgta aactaactgt
    22441 tttttgagtt aatttttgta aaactgctta tttaataagc atatggttga atatagggaa
    22501 attatggaat tttgtttcag aatttcggac attcgataaa tttccattcc ccgggtttga
    22561 ggttttctgt ttccaaatgt gcgaatcgcc ggcggtgaag gtgttgcacg cggttgccgg
    22621 cggcggcaac catgcgtttg acttggtggt attttccttc ggtaatggtc agcagcaggg
    22681 tggtcggatt ttccaaaacg gcatcggcgg cacaaacggt ttcgttttcg tcgtggagcg
    22741 gcacgccgtt tttcaaagtt tcgcagagcg tttctcctgt agggtgtttg agcgttactt
    22801 cgtatagctt gggaattttt ctgctcggcg aagtcaggtt gtggttcagt ttgccgtcgt
    22861 tggtaatcag caatacgccg gtcgtatccg catccagcct gccgaccgcc tgcatatcga
    22921 tgttccgcat attgtcgggg aacagactga atacgctgcg gtagtgtttc ggtttgtgcg
    22981 aagtttcgta atcttcaggc ttgttgagca ggatgtagaa atagggttcg ggaacgacgg
    23041 ttactgcttc cccgtcaata tccaacgttt cgacgtatga ggaatcgatg tctgcatcgg
    23101 tgtcgtccat gcaggttccg ttgatggaga cgtagccgcc cctaatcagc cattggcact
    23161 gcttgcggct tcctatgcct tgatattgca ggtatttgat gagtttcatg atggtatggg
    23221 aaatgtggga tgaaaaagac aggactgaga aagtcctgtc atgtgccgta tccgaatcag
    23281 cttccaatca gtttgaccct ctgtcccgga ttgatggtgt tcaggttggg attgagccgg
    23341 cggatgtcgt cgatatggat attgaagcgc gcggcgatac ttttgaaggt gtcgcctttg
    23401 cgcgcggtgt aggatacttt ttcaatacgg gtttgggcag gggctgcctg cgccaggcgg
    23461 agtacctgtc ctttttggat ggtgttgcct ttgatgttgt tggcgacaat caggtcggct
    23521 acgctgacgt tgtagcgttt ggcaatgttg aacagggtgt cgccttctac aacgcggtgg
    23581 atgctggcat ggagcgggga ggtttcggct tcgccgtcgg cagctttggc tacgcgcgtt
    23641 tccaaacgtg cccggcgttt gccgtcggcg gcaacggtat gttgcggaga tgaggaaatg
    23701 ttgtgttcgg caactgtgtc aggcgtgccg atgacggcgg agatggtttc ttcagcctgc
    23761 cggcgcaggt tgtttcgggc aacaagctgc atgagttcgt ctgccgcctg catgtcgttt
    23821 ggggggacga cctgcgcgag tgttgcggtt tgggtttcag acggcatggc agtctgtttt
    23881 tcggtttgag gttgcgctgc ggctgtcgca agggcgggtt cggcaatgcg gacaagggga
    23941 tcgggttccg tacggacggt ttcttgcggc aaaggtgcga cggtaatgtc cgctgtctgt
    24001 gcggcggcgg gttggattcg ggcaatgctg acgttcaccg tgcctgccgg catattggaa
    24061 cggtaggtgt cgggcgtatt gtcgatgtcg atggaaacga cggattccga tgccgtatga
    24121 agggtcttgc cgttcttggc gacaaggatg ctgcgtcctg cgttgacaag gttgccgttc
    24181 aggttgttga ggcgtttgat gtcggcaatg ctcatgccgg ttgccgtcga gatgtcggac
    24241 aggctggttt tggcggcagg cgtatagact tcccatgaaa acaggctgtc gggtgcggcg
    24301 ttgaggtagt tgctttggaa ggtttggacg gacgcgacag gaagcagcag tttgcgtttg
    24361 tttttgggga tgaacgcggg gacgttgaat gcaggattca gggcgagcag ctcgctttgc
    24421 gtgatgccgg caagccgggc gatggcttcg ttgtcgagcg gacggcccgg ttcgactgcc
    24481 tgaaaatagg gtttgttgtc tatgtcgctg atattcatgc cgaaagattg gggggtggca
    24541 ataatgttgc gcacggcgag cagcttgggg acatagttgc gcgtttcgtt gggcatacgc
    24601 aggttttcgt aggtcggttc gagcccttgg tcgcgggcgc ggttgacggc gcgtccgacg
    24661 ttgccttcac cccagttgta ggcggcaaag gcgagcggcc agtcgccgaa cagtccatag
    24721 agatattgca gatagttgag tgcggcatcg gtggctgcgt aaacgtcgtg cctgccgtcg
    24781 taaaccggtg ttttttccaa gccgtaatgc ctgccggtag cgggcatgaa ctgccacagg
    24841 cccgatgcgc cgacgtgtga tttggctttg gtgacgaacg cgctttcgat gaagggaagc
    24901 agggcggctt cggcgggcat attgcgtttt ttgacttcgt tggcgatatg gtacatatag
    24961 ggtcggctcc ggttgacgac cctgtcgaaa tagctgcggc ttgcgatgaa tttgctttcg
    25021 tggcggcgta ccagttcggg attgacttcg cccatccgga agccttggcg cagctcgtcc
    25081 cacaggctgc cggattggaa atattgtttt gtcggtggca ggtcgagtat tgaagagttt
    25141 aaccgcataa tcgctaaacc gacttggtgt gatgaggtgt tttgggcata taggaaaccc
    25201 ggacaaacgg acagacctga tgcggtcagg gcgatggttt tgagtttgga catgatttat
    25261 actcggcgga ataaacatat tttatcgtcc gatggtactg tttgactgaa aaagcgtcaa
    25321 gttttacagg aactttatgc ggattcggaa atgggcggcc gatgtgtttt ttgacagaaa
    25381 tcctgtttgg aataaaatat cggcaggcaa tctgtttgaa acggacgcaa tatggatgca
    25441 tggtttgaag atacggcgat ggggcggtat gttgcaaaat tggaacagga cttctttaat
    25501 cggtatgtgg cgtcataccg tttttcggga atgtgtgcgg ttcaggtggg cggtcagtgg
    25561 ctgagcctgt ctgaagacat tgtctgtgtg ccgcgagata tgtcgatgtc ggcggagaat
    25621 atggctttgg cggatgtttt tgcggatatg ctgcttttgc cgcatacgct ggaatgcggc
    25681 gtaccttcgc aaatcctgtc ggaagcgcac cggatattga aaccgtccgg acgtttgatg
    25741 ctgacgggtt tcaatccgta ttcgctttgg ggattctgcc gttggtttga cggtgtccgt
    25801 ctgccggaaa aacggttttg tctgccgctg cccgagttga aaaggcagct tgccgatgcc
    25861 ggtttcgata ttgaatttgg gaaattcatg gtgtatctgc ctcccgtttc gtcgctcggg
    25921 caaatacgct tttggcggtt tatggaaaag gcgggcgacc gttggtggcc gcaatgtgcc
    25981 gcagtgtacg gtttggtttt ggtcaaaagg gcggcgggcg taacgcccct gcccgcgtgg
    26041 gacgggtatt ggggcggcaa agcccttgcg gcgggtgcgg caagggttgc ggattaaggg
    26101 acggccgtct ttcagacggc atctgtcaga agaaatgtcg ggaggggtta tgccggaatt
    26161 gccggaagtg gaaacgacgt tgcgcggcat cgcgccgcat attgaaggaa aaacggtgga
    26221 agccgtgata ttgcgccaac tgaagctgcg ctggcagatc aatcccgatt tgggggagat
    26281 tttgtccggc cggcaggttt tgtcctgcgg caggcgtgcc aaatatctga ttgtccgttt
    26341 ccaaacgggg attttgctga ttcacttggg gatgtcgggc agcttgcgga tctttacgcc
    26401 gtcggacgga cgtatcggca ggccggacag acacgatcac gtcgatattg tgttttcaga
    26461 cggcacggtc atgcgttacc gcgatccgag aaagttcggc gcgatacttt ggtatgaggg
    26521 aatcgaagaa cgtcatccgc tgttggaaaa actggggccg gagcctttgt cggaggcatt
    26581 ttgcacggat tatctgtatg cggggctgaa ggcgcagaaa cgcgcggtca aactcgctct
    26641 gatggacaat accgtcgtgg tcggcgtggg caatatttat gccaacgaga gtctgttcag
    26701 ggcgggcatt tcgccccacc gtcctgccaa ccgcctaaaa aagaaagagt gcgcggtttt
    26761 ggttgaaacc gtcaaagcgg tgttgcagcg cgccattgaa acgggcggca gtacgctgag
    26821 ggattttgtg gacagcgacg gcaaaagcgg ctactttcaa caggaatata cggtgtacgg
    26881 gcggcacaat cagccgtgcc tccggtgcgg cggtttggtt gtgaaagaaa ctttggggca
    26941 gcgcggcacg ttttattgca cgaactgtca gaaatagggc ggaaaacggt ttctgacggc
    27001 attttatcgg tatgccgtcc gaacgtttca acaacaaaca ccgattatcg ggaaagaatt
    27061 gcccatgtct tcaaataaag cttcattttt tacacgtctg cgccgcttgt gccgcttgac
    27121 ggtctggctg ttcaaaaccg gcaaaaacct gcgcggtatt gacggcggct gccccaagtc
    27181 gcgcaatcgg gcagttatag cgttgggcaa gggcgctttg gcggctttgg atatcggatt
    27241 ggaggtgggc agacccgcac ccgaacatcc gaacggtgtc ttggttgccg ccaaccacgt
    27301 gtcctggctg gatattttcg cgatgagcgc ggtttatccg agtagcttta tcgccaagca
    27361 ggaaatcaaa agctggccgg tattgggcaa gatggggcag aacgcgggaa cggtgttcat
    27421 caaccgcaat tcgcggcgcg acatcgaacc gattaaccgc gccgtctgcg aaaccttgca
    27481 acgcggtcaa aacgtcagtt ttttccctga ggcgcgaact tcctccggac tggggctcct
    27541 gccgttcaag gctgcgctgt tccaatccgc catcgatgcg ggggcaaagg ttttggcggt
    27601 cgcactgcgt tattatgacg aaacgggaaa aaggacggcc cgtccgtcgt atgccgatgt
    27661 cggtttgccg acttgcctgt ggcgaatcgt gtctatgaaa aaactgacta tcaaagtcga
    27721 tttcgtttgc gtggcggacg cggcggaaag cgaagaccgt tatgctttaa aagataaaat
    27781 cgaagaaagc atccgtgccg ttgtcgcagg cgatgcggat gttgccgtct gaaagcggtt
    27841 gtcggaatgt ggcggtatga ttcgcttttg cggatgcctt aaggctgcgg taacgggagt
    27901 acctcaaaag aaatgccgta aggatagttt gttgtatagt ggattaaatt taaatcagga
    27961 caaggcggcg aagccgcaga cagtacaaat agcacggcaa ggcgaggcaa cgccgtaccg
    28021 gtctaaattt aatccactat atctatggaa aatgccatct taacttgtgt tcagacagca
    28081 tattttatat gggattaacg gcggaacctg ccgccgtcga aatcatcgaa agcctcataa
    28141 gccgcccggc attggatttt ctgttccgca taaggcaggg tgcggaatgc tttgcgcgcc
    28201 tttttgtttt cgcgcaattc ctgcacgttg ccttgcgatc ggtaccacgc ggcacggcgt
    28261 tccaaatatt tcctgcattc ggggtgcagc atctgcttgt ttgccttgct gatatgtttg
    28321 gtatgtcggt gggcgggttc tgacgtaccg gcagcggcag ttgttgatac cagtcccagt
    28381 attgcagaca acagtatggc aagcatcttt attgacggac atgacgatcc tttcagtttg
    28441 taattccttc cagtatagga tggaaggatg gaacgggcaa actgtcggca gtttcagact
    28501 cattcagccg acaaaacttg ccgtttgata tgcaaattca aataacatat taatctcaat
    28561 aataaattcc aatcggtaag aaaatggaat ttgtcggcgg cggcgtggca aaaatcatac
    28621 tttgcaaaat ttaacaattc gcaggggcgg aaaaccggaa gttttccttt ttcgtcggaa
    28681 aatccttatt tcatcgcctt gtagccggag ccggtcaaaa ggcaaaaaat ttacccgttt
    28741 tttatcggta aagaattatc agataaaaca aatgttatag gaaaaatacg acaggcgggt
    28801 tttatcgcgc attgcctgaa actgaaaaat gcaatcgttg tcaagactgg agaaaatgct
    28861 aaaaatccac tatattgtct gccttaattt atttgaaaag accgtgtctt gaatatcaag
    28921 agtggaagag gaagcgatga atacaccgac tgatttaaaa gtaaccaaac gagacggaag
    28981 attggaagcc attgatttgg ataagattca ccgtgtcgtt acttgggcgg cggaaggatt
    29041 ggaaaatgtt tccgtgtcgc aggtcgagtt gaaatcgcac atccagttct acaacggcat
    29101 ccgcaccgac gacatccacg aaaccatcat caaagccgct gcagatttaa tttcggaaga
    29161 taccccggac taccaatacc ttgccgcacg tttggcgatt ttccatcttc gtaaaatagc
    29221 ctacggcgaa tacgagccgc cgcaccttta caaccatgtt aaaaagctca ccgatgccgg
    29281 aaaatacgac aggcatatcc ttgaggatta cagccgcgaa gaatttgacg aactgaacgc
    29341 ctatatcgac cacgaacgcg atatgtcctt ttcctatgcc gccgtcaaac agctcgaagg
    29401 caaatatctg gtacagaacc gcgttacccg ccagatttac gaaacgccgc agtttttata
    29461 tgttttggtg gcgatgtgcc ttttcagcaa atacccgaaa gaggtgcgct tgggttacgt
    29521 caaacggttt tacgatgccg tttccacatt caaagtatcg ttgcccacgc cgattatgag
    29581 cggcgtgcgt acgcctacgc gccagttctc aagctgtgtg ctgattgaat gcgacgatag
    29641 tttggattcc atcaatgcca ctaccagcgc gattgtgaaa tacgtttccc aacgcgcggg
    29701 catcggcatc aatgccggac gtatccgcgg gctggacagc gaaatccggg gcggtgaagc
    29761 ccggcatacc ggctgcattc ccttctttaa gatgtttcag gcggcggtca aatcttgttc
    29821 gcaaggtggc gtgcgcggcg gcgcggcaac cttgttctac cccttgtggc atattgaagc
    29881 cgaaagcctg ctggtgttga aaaacaaccg gggcgtggaa gacaaccgta tccgccagct
    29941 tgattatggc gtgcaaatca accgcctgct gtacacccgc ctgattaagg gcggcaacat
    30001 tacgctgttt tcgcccaacg aggtttcggg gttgtacgaa gcgttttttg ccgaccaaga
    30061 cgaatttgag cggctctata cgaaatacga gcaagacccg aacatacgca agcgtatcat
    30121 tccggctgcc gacctgtttt ccacgctgat gcaggagcgt gccgggaccg ggcgcatcta
    30181 cattcaaaac gtcgatcact gcaatacgca cagcccgttc gatccgcgcg ttgcgcctgt
    30241 tcatcagtcc aacttgtgta tggaaatcgc cctgccgacc aaaccgctgg acaacatcaa
    30301 cgaccctgac ggcgaaatag ccctgtgtac actgtccgcc ttcaacttgg gtgcattaaa
    30361 cagcttggac gaattggaag ggcttgccga tttgaccgtg cgcgcactcg atgcactttt
    30421 agattatcag ggatatccgg tagaagccgc gcgtacctct actatggacc ggcgttcgct
    30481 cggcatcggt gtcatcaact acgcctacta tctggcgaaa aacggtgtcc gctacagcga
    30541 cggttccgcg ctcggtctga cccaccgtac ctttgaagcc atacagtatt acctgctcaa
    30601 agcatcggca aaccttgcca aagaatacgg agcgtgcacg ctctttaacc aaaccgttta
    30661 ttcgcaaggc aaactgccca tcgacaccta caaaaaagat ttggatgccg tctgcggcga
    30721 gcctttgcat tacgactggg aaagcctgcg tgccgacatc gtcaaatacg gcctgcgcaa
    30781 ctctactctg accgcgctca tgccgtctga aaccagctcg caaatcgcca acgcaaccaa
    30841 cggcatcgag ccgccgcgcg gattggtaac ggtcaaagca tcgaaagacg gcattttgaa
    30901 acaagtcgtg ccggagtttg aaaccttgaa aaatgcctac gaaaccctgt ggcagcttcc
    30961 gggcaacgaa ggctacctga aacttgtcgg cgtaatgcaa aaattcgtcg atcaggcgat
    31021 ttccgccaac actgcttacg acccgggcaa attcgaaggt aacaaagttt ctatgaaaca
    31081 aatgctcaaa gacctgctga ctgcctacaa atacggcgtc aaaaccctgt actaccacaa
    31141 cacccgcgac ggcgcggacg acacgcagac cgatattcaa gatgacggct gcgctggtgg
    31201 ggcttgtaag atttaaaaca ggttaatttt tgaaatatat ccaaggcttc tagatagtag
    31261 gaggcttcaa ggattttctt aaattagtgc ctactactat atgaggctaa gttgttattt
    31321 tccagaaaag gaaaagtatg gatgtcattc ggtcattctt agttgaatat aaattttata
    31381 tatttattag tgttctttta gtatttgcta ttttaggatg gcactttatt gatagaattc
    31441 aacatgtttt atatggtgaa ttaaaaatta atagaaaagt cgactaattt ttaaaaagag
    31501 agacgttatg tcctgcgaac acctagtcat gtcatacagc acctttccca aaaccaaaaa
    31561 cgacgcgctg aaagagccga tgtttttcgg tcagccggta aatgtcgccc gttatgacca
    31621 gcagaaatac gaggtatttg aaaaactgat tgaaaaacaa ttgtcgtttt tttggcggcc
    31681 ggaagaaatc gacgtgtcgc gcgaccgtat cgactacgcc aacctgcccg aacacgaaaa
    31741 acatattttc atcagcaatc tgaaatatca aacactgctc gattccatcc aagggcgcag
    31801 tccgaatgtt gccttgctgc ctttggtttc gattcccgag ctggaaactt gggttgaaac
    31861 gtggagcttc agcgaaacca tccactcgcg cagctatacc cacatcatcc gcaatattgt
    31921 gaatgatccg tcggtcgtgt ttgatgatat tgtcgaaaac gaatacatta ccgcccgcgc
    31981 cgaagacatt gcctgctatt atgatgattt aatcgaatac acccaatatt acaacctgtt
    32041 gggcgaaggg gtgcacaatg tcggcggaaa acccgttact gtgtctttgc gcgggttgaa
    32101 gaaaaaactc tatctctgcc tgatgtgcgt caatgtgttg gaagccatcc gtttctacgt
    32161 ttcatttgcc tgctcgtttg cttttgctga gcgtgagttg atggaaggca acgccaaaat
    32221 catcaaactg attgcccgcg atgaagccct gcacctgacc ggcacgcagc atatgcttaa
    32281 tctgatgcgt tcgggtgttg atgatccgga aatggcggaa attgccgccg agttgcagga
    32341 cgaatgtttc caactcttca aaaaagcggc ggaacaggaa aaagaatggg cggcatattt
    32401 gtttaaagac ggttcgatga tcggtttgaa caaagaaatc ttatcccaat acgtcgaata
    32461 tattaccaat ctgcgtatgc aggcggtggg gctgtcggcc ggatttgaag gcgcaaatca
    32521 aaacccgatt ccgtggatta atgcgtggct gtcgtccgac aacgtacagg tcgcgccgca
    32581 ggaagtggaa atatcctctt atttgattgg tcaaatcgat tctgaagtga atacggatga
    32641 cttgggcgat tttgagttgt aaatgttttt tgaaaaaatg ccgtctgaag ccggatgttt
    32701 cagacggcat ttgtgtgttg tatgatggga aaaacagcag gtaggatcgg ttattgttcc
    32761 ttttattttt gattggtcgg aaatggcgcg cattggtacg aataagggcc tttttgagct
    32821 cttggagggt gagaccctgt tggaaggctt ggaacggacg gggcatatgg tggaatatca
    32881 gtgccgaagc ggatattgcg gatcgtgccg ggtaaagatt ttggagggaa gcgttactta
    32941 tcgggagccg cctttggctt ttttggggcg ggacgagata ctgccgtgct gctgttgcgt
    33001 cgagggggat gtcaggctgg attgcggttt ggcgggggag gatgaggggc tgtctgacag
    33061 tttgttgaaa taaaagccgt ccgaacattg gttcggacgg ctttttgacg gtattaccgg
    33121 ttatgcctga tgagtcggta aaaggtttcg ccgtcttgga tataacccag ttctacccgg
    33181 gcgatttccg aaatggcttc ttgaccgttt tccaaatcat agacttcggc ggcaagggaa
    33241 tgattgcgta gggcgagtgt ctggtttttt tcttcttgaa cggcaatctg ttctctcaga
    33301 ctgctgttgc gtccgatgct gcctttgccg aaccacaggc tgtattggca acagacaagt
    33361 gcgaaggata aaacgacagt tacccacttc atcaacgttt ccttactggt tgaaacccca
    33421 ccactcggat gtccgtcctt cgtggcggta aaattagact ttatttggga gaggtgtaac
    33481 ctcttccaaa tcaggtcaac acacagggcg acgcttcatg tgttgccctg tgtgttgaaa
    33541 catacatcca cttttttatt tatttgccca gttggtagaa tgcggctttg ccggggtagt
    33601 aggcggcttc cgccaattct tcctcgatac gcagcagttg gttgtatttc gccatgcggt
    33661 cggaacggct caaagaaccg gttttaatct gcatacagtt ggtggcgact gccaagtcgg
    33721 caatggtact gtcttcggtt tcgccggagc ggtggctcat cacgctggcg tagcggttgc
    33781 attttgccag atcgacggct ttcagggttt cgcttaaagt accgatttgg ttgactttga
    33841 ccagcaatgc gtttgctacg cctttttcga tgccttcggc aagaattttc ggattggtta
    33901 cgaacaagtc gtcgccgacc aattgaactt ttttgcccaa tttttcggtc agcagtttcc
    33961 agccttccca gtcgttttcg tccatcccgt cttcaatgga aataatcggg aattcgttaa
    34021 ccaagccttc caagtattcg gcaaattccg cgttggtgta ggagcggcct tcggcttcca
    34081 agtggtattt gccgtctttg tagaactcgc tggacgcgca gtccaatgcg aataatacgt
    34141 cttcgcccgc cttgtagccg gcggcttcgg ccgcttcgac catcagttgc agggcttctt
    34201 tgtggctgtt caggttgggg gcgaaaccgc cttcgtcgcc gactgtggtc gggaagcctt
    34261 tactgtcgca cagttttttc aaggcgtgga aaatttccgc accgcagcgc aacgcttcgc
    34321 ggaaagattt tgcgccgacg ggcataatca taaactcttg gatgttcagg ctgttgttgg
    34381 cgtgttcgcc gccgttgatg acgttcatca tcggtacggg cagggacatc ggacctgcgc
    34441 cgcccaagta gcggtaaagc ggcaggcctg agtcttcggc agcggcgcgt gcaaccgcca
    34501 tagagaccgc caaagtcgca ttcgcaccca aattgccttt gttttcagta ccgtccaatt
    34561 cgatcatgat ttggtcgata taagattgct cgttggcatc gataccgatg agggcttggg
    34621 cgatttggtt gttgacgtgt tcgaccgcct tcaatacgcc tttgccggaa tagcgggatt
    34681 tgtcgccgtc gcgaagttcc aaagcttctt tctgaccggt ggatgcgccg ctcggtacgg
    34741 ccgcacgtcc cattacgccg gattcgagca atacatcaca ctcgactgtg gggttgccgc
    34801 gcgagtccaa aatttcgcgg gcgaaaatat caacgattgc gctcatgaat gttctccaag
    34861 tgaagtgaag ggaggtaaaa aatgggaggg cttcagacgg cctctttgcc ggaaagccgc
    34921 caacttaaaa ctgctgaaag tagggcggtt gccatgaaag tcatgctgga aataccgtgc
    34981 cacattccga aggatccgcc tgcaaccgac aacagccaat tttcatgccc gtatttcagt
    35041 gcctcgataa cgggtgtaac caaaaattgg ttggcggcaa gggcggacaa taaaaaaagc
    35101 agcagggcgg tttgccgccg ggttagggcg gcaaaggcgg cagccagtac cgtgccccaa
    35161 acggcaagcc cgctccaaga gaggatgtcg aacaatacgc cggcaatttc gcccgcctgc
    35221 attttgggca gcattttgaa cagcaccggt gcggcgatat aacctgccat aatctgcata
    35281 ccgagccaca aggttgcggc atacaggctg atttttcgga aagtctgcat cataaggcga
    35341 aaccgtgtta ctcgattgtt aaaatcggtt gtgatttgat taaatcgtcc aatgctttga
    35401 tgcggattaa aaaattttct aaaaggtgta gcggcagcgc gctggggccg tcgcattttg
    35461 ccagtttcgg atcggggtgc gattcgagga acaggccggc aaggcgggtt gccatgcctg
    35521 caagtgccaa atccaaagcc tgtgcgcgac gaccgccgga tgcggcagaa ccggcatcgc
    35581 gggtttgcag ggaatgggta acgtcgaaaa taaccggcag gttgccgcag gtttgtttca
    35641 tcacgccgaa accgagcata tcgacaacga ggttgtcgta gccgaagctg ctgccgcgtt
    35701 cgcataaaat cagcttcccg ttgccggctt cgcggaattt ttccacgatg tttttcattt
    35761 gcgaagggct gaggaactgg ggttttttga tgttgataac attgcccgtt tccgccattg
    35821 cggccaccaa atcggtctgc cgcgcaagaa aggcgggaag ctggatgaca tcgcacactt
    35881 cggcgacggg ttggcattga tggggttcgt gtacgtcggt aatgacgggg atgccgaact
    35941 ctgctttgac tttttcaaaa atctttaagc cttcttccaa gcctacgccg cgataggaat
    36001 ggatagacga gcggtttgcc ttgtcgaaag aggctttaaa gatatagggg atgcccagtt
    36061 tgcgggtaac ttcgacgtaa tgcgcacagg tttggagggt ggaatccaaa tcttctaaaa
    36121 cgttgatgcc gccgaatagg acgaaaggcg aattgttgcc gagggtgatg tcgttgattt
    36181 taatatccat aatcgatgtc cgtttgcggg attacagtac ggtgtgcggc ggctcgtagg
    36241 agatgatgtc gcggatttta ttgtttccgt ccactaagac gactttgggt tcgtgtgcgg
    36301 cgatttccgg ttcggaaagt tggatataag acataatgat gacgatgtcg cctttctgta
    36361 ccagcctggc tgcagcaccg ttcaggcaaa tcacgccgct gccgcgtttc cctgcaatgg
    36421 tataggtttc aaaacgttcg ccgttgttgt tgttgacgat ggcgactttt tcgttggggc
    36481 agatgcctgc cgcgtctaac aggtcttgat cgacggtaat gctgccgacg tagtttaaat
    36541 cggcttcggt tacggtggcg cggtggattt ttccgccaag tatagtacgg aacatgtatt
    36601 tccttttggt ttgtgtgcgg tctgacgggc gtgatgccgt ctgaaaatct gaatgccgta
    36661 ttatagtgga gtttccttca aattcataat ggcaaggctt cgggcaggga agctgttttc
    36721 agacggcatt tcatttttgc cgattggcaa tccggctcaa ctctcccaat cgattgagaa
    36781 aatgctgccg tccaaatgca gtttgacggc aaggatttcg tcgctttcca tattggcggg
    36841 catataatcc ataacggcaa aagccggatt ttttgcccat aagctgatat agatcaactg
    36901 catagtgcgg acaaattttg ccgcatcacg gcttgtttgg gtatcttcgg tgtgaaagtc
    36961 gatatagcgg ctgtcccgtt tcaaatattc cgtcagtgcg gcgcgtgcag catcgtcggc
    37021 tttttccaat tcggacagga aggcggcaaa tgcatccaat gtttcggttg gcaggctgtt
    37081 ttcattttct gcctacagga atacctcgac aagattgcct ttgacggtca gctcgtcttc
    37141 ccagaggaca tcttcttctc cgtcaaaacc ttcggtggac agttgttgga aataggggtg
    37201 aaaaatcgac atgattgttc cttttggtga ggattggggg attgtatgcc gtctgaacgg
    37261 tgttcttcag acggcatcgt tacatcaatt tcctttgggc aggcgtttgc cttgcagggt
    37321 ttttagtacg gttttgacgg tattgatcct gacctcgatg ttttccattt ccgctgcaaa
    37381 cctcaatttg tctgcccctg ccagccggta ttttttatcc gtctgaatca gcaggataat
    37441 ccccgtcggg tcgatacagt ggtgtttgcc gaaggtaacg gttacggctt cacttgtggc
    37501 atcgatggca tcaataccca gttcttttgc cgcaagccgc aggtggtggc tttcaatgag
    37561 ggttttgacg ggttgttcgg gcaggccgaa gcggtcgacg agttcttcgt gtatggcgtt
    37621 gatttgctgc acggtttcgc atacggcgag gcgtttgtag aggacgaggc gctcgtggat
    37681 gtcggggcag tagtcttcag gcagtaatgc cgggctgtgc agtttgattt cggtggtaat
    37741 gcccaacggt gcgtcgaggt cgggttggcg gccttttttg aggtcgcgca cggcctgttt
    37801 gagcatttcg gtgtagagcg tgaagccgac ctgcatcatt tcgccggatt gtccttcgcc
    37861 gaggatttcg cctgcgcctc ggatttccaa atcctgcatg gcgagggtga aacctgcgcc
    37921 gagttcgtct gccgccgcga tggcgtcgag gcgtttttct gcgtctttgg taatgtattc
    37981 gggcgtgagc aggtaggcgt aggcttggtg gtggctgcgg ccgacgcgtc cgcgaagctg
    38041 gtgcagttgg gccagcccga atttgtcggc gcggttgatg atgatggtgt tggcgttggg
    38101 gatgtcgatg ccggtttcga tgatggtgga acagagcaac acattgaatc tttgctgcaa
    38161 aaagtcgcgc atgacttgtt ccagctcgcg ctcgcgcagt tgtccgtgcg ccacgccgat
    38221 acgggcttcg ggcagcaggg tttccagccg ctcgcgcata ttctcaatcg tatctacttc
    38281 attgtgcagg aaaaatacct gtcctccgcg tttgagttcg cgcaacacgg cttcgcgcac
    38341 gctgccttcg ctgaacggtt tgacaaaggt tttcacggcg aggcggcggc tcggtgcagt
    38401 ggtaatcagc gagaagtcgc gcagaccttc gagcgccatg ctgagggtgc gcggaatcgg
    38461 cgtggcggtc atggttagga tgtcgacatt ggcgcgcagg cgtttgagct gctctttctg
    38521 tcgcacgccg aagcggtgtt cttcatcgat aatcaataaa cctaagtttt tgaattttat
    38581 gtcgtcctgc accaatttgt gcgtaccgat aacgatatcg acagtaccgt ccgccatgcc
    38641 ttcgagcgtg gctttggtgg ctttgctgtt gttgaaacgc gaaaggctgg cgacttttac
    38701 ggggaaatcg gcgaaacggt cggcgaagtt ttgcgcgtgc tgctcgacca gaagcgtggt
    38761 cggggcgagt acggcgacct gtttgccgcc catcaccgcc acaaacgcgg cgcgcagggc
    38821 gacttcggtt ttgccgaaac cgacatcgcc gcacacaagt cggtccatcg gcttcgcctg
    38881 cgtcaaatct ttaatcacgg cggcgatggc ggcggcctgg tcttcggttt cctcgtagcc
    38941 gaagccgtcg gcaaacgcct gatagtccga ctcgttgatt tcaaacttgt gtcccgattg
    39001 ggcggcgcgt tgggcgtaga ggttgagcaa ctcggcggcg gtatcgcgcg ctttttcggc
    39061 ggctttgcgc ttcgccttgt tccacgcgcc gctgccgagc ttgtgcaggg cgacgctttc
    39121 atgcgcctga ccggagtagc ggctgattaa atgcagttgc gaaacaggca cataaagctg
    39181 cgcttcgccc gcgtattcga gcaacatcat ttcgttggtt tcgccgccta aatccatcgt
    39241 taccaagccc gtataccgcc cgatgccgtg ttcttcgtgc acgacgggat cgccgatatt
    39301 gatttcggca aggtcgcgca acaggccgtc tgaaacagcg gcgtgtttct tacggcggtt
    39361 gtggacgcgg gaacgggcga cgtattggta aagatcggat tcggtgatga cggcgatggc
    39421 actttgagcg gcaaccgctt tgccctctcc ccaaccctcc cccacggggg agggggcaga
    39481 ttgctgtcgg gtttgcccgt gcagatcact tgcgacgaga tacaggctgc tttcggttga
    39541 aacggcggct gctttcaggc cgtctgaaac ggcggcggca ctttgttccc tctcctgcgg
    39601 gagagggctg gggagagggt ttgttgcgga tgcggaaaat tcggtttggt cggtaaccgc
    39661 tttgccttct ccctcggagg cgggaacagt ttgctgttgg cttgaagatt gcagtccgcc
    39721 cagtttgaat ccgtaagcca acggcgcgac cgtaatcatc agcggctcgt gcgccgacaa
    39781 gaagccctgc cagtcggaca caggtttggc tttcaaaccg ttttgctgca agaaaccgag
    39841 catggtttcg cgccgtccca aactttcggc acacagcaaa atccgcccgt caaaggccgt
    39901 ctgaaaatcc ttcaatgcct gcaaaggctc gtcggattgg cggttgacgg caaggtcggg
    39961 cagggagtgt gccttgccgg aaacatcagg cagaacttgt ccgtagtttt tcagacggcc
    40021 tgcgaacaca tcggcagaga gatacaaatg ctgtggaagc aaaggcggat aggtttcgtc
    40081 gccctgcgcc atggcgtaac gtgatttaac gtcgttccaa aagcggtttg cctcggcatg
    40141 aacatcgccc aaagagacaa acagcgcatc ttcgccgata tagtcaaaca gcgtttccag
    40201 ctcgttttca aaaaacagcg gcagataata ttccacgccc gcgccgaagt ggccgttgct
    40261 gacggctttg tacacggcgg catcgttcgg attaccgtcg acttcctcgc ggaagcggct
    40321 gcggaagatt ttttgcgcct cgctgtcggt ggggaactcg tgcgccggca gcaggcggat
    40381 ttcggaaacg ggggaaatgg tgcgctgcgt gtcggtatcg aaggttttaa tgctgtcgat
    40441 ttcatcgtca aacaaatcga tgcggtacgg cgtttcgctg cccatcggga acaaatcgac
    40501 aatgccgccg cgcacggcaa actcgcccgc cgcgacaacg tgggaaacat gattgtagcc
    40561 cgcatccacc aaatcggttt tcaggcggcc tatatccaaa gtctgccccg ttttcagcca
    40621 aaacgtgcgc cccgccaaaa acggcacggg cggcagcttc tgcatcgccg tggcaaccgg
    40681 tacgaacaac acgtccgccg cgccgctttt aatctgccac aacgccgaca gccgctccga
    40741 caccaaatcc tgatgcggag aaaaacgctc gtaaggcagc gtttcccagt ccggcaggaa
    40801 caccgccgtg tcgtgcggac ggaaaaaccg ccatgccgtc tgaaggcgca acgcctgttc
    40861 cgcatcttgg gtcagcacgg ctttgagccg cttgtgcggc aaataacgcg ccaaagccaa
    40921 gggcagcgag ccttgcgaaa gattgaacca acgggatttt tcacggggtt tggggatggg
    40981 gcaggtcata gtgcggcaat cggcggacag tgatgcaaac ggtcatttta gcacgtgagg
    41041 gtgcgcgatg ccgtctgaaa ggccggcgga ttgttgcccg gtgcactttg tggtaaagtt
    41101 tagaaaatta actctaaact gtggcaggcg gtttgaccgg ccgccgatta atgggaaccg
    41161 acagaggaag ccgatgatac acaccgaacc gagcgcgcag ccgtcaacca tggacacggc
    41221 tgctttttta aagcacatcg aatccgcatt ccgccgcatt ttttcagacg gcatcgacct
    41281 gatgcgatac ctgcccgaag gcaaatggct tgccttgaag caggcgggtt tgctgttgcc
    41341 cttcctcgac aaaaaacacg gcgggcgcaa gggcagtcag tttgaaatcc aagaagtcct
    41401 aaggattgcg gggcattacg gcgtgcccgt tacgctgcgt accggcatcg aaggcgcgct
    41461 ggtgttgcag cctctgcaag agttcggcgg cgaagcgcaa gtcgcacaag gtttggacat
    41521 gattttcaaa ggcgaaggcg gcggtttggg cgttaccgaa cccgaaacct ccggcgcggc
    41581 gattgcacgc gaaatgcagt cctgctacga atataccgac gaacaaacca tttacgtcaa
    41641 cgccgcgaaa tactggcagg gcaattcgca aagcgacttc ctcctcgttg ccgccaaaga
    41701 gcgcaaaaac ggcaaactcg ccaaagtcat cgacctgctg ctcgtcccca aaacatacat
    41761 ccgctgcgaa accctcgcat ccgaaggcct gcgcgccgtc cgttacgccg tcaaccgcat
    41821 cgatgccgaa atgcctgcaa ccgccgtgat gaaactctcc cggggcgacg ctgccggttt
    41881 gcgcgcgttc caaaacatct ttatccgcag ccgcctgcaa ctgatcggca tgacgcacgg
    41941 cattatggaa tacatccttg acaacctgaa ccgatatgtc cgcaacgata tcagattcgt
    42001 cgattacgaa cgccgcgaaa tccaacgccg ccatcaggtt tccgaaatcc tttaccgcta
    42061 cgtctgccat tccgtttcgc ccgtcgcgcc cgtcgcccat caattgatgg aggcgaacat
    42121 cgtcaaaacc ctcgccacgg aatacactta cgccgccgcg caaatgttgc aaaaactctt
    42181 gggcgcgaag ggttttgaac gcggacaccc agccggcaat atcgccatcg atatccgccc
    42241 cttcacgatt tttgaaggcc cgaacgatat gctttatgcc gaaatttacg accagttcgt
    42301 ccgcgccacc gccgaagaaa aagaagcagg cattaagttg gacaaaaacc aaaccctgct
    42361 cgaccgcctg caaaccgatg tccgctttgc cgccgttgcc cgcgactacg ctttgcccga
    42421 agacatccgc agcttcctgc aggaacacac cctgaccgac gcctgcgccc tgcaaaaagt
    42481 cttcatcggc aaaatcatcg cccgactctt tgtcttcgta caggaggaac acgaagacac
    42541 cacagccttc ctgctgaacg acatccgcaa agatatattg gactgccgat attgcggata
    42601 gcgggcaaat acggcggcag gcagatttcg ggcggcgcaa aaggcaacgc gcggcattct
    42661 gcaaaaccgc cgattgcggc ggataccgca tttcgcgcct cacccttgtg ccgctttccc
    42721 acttacccta tttgttcgga atgtgtttat ggaaaagaga aaaatactgc cgctggcaat
    42781 ttgtttggcg gctttgtctg cctgtacggc gatggaggcc cgcacacccc gggcaaatga
    42841 agcccaagcc ccccgcgcgg atgaaatgaa aaaagaaagc cgccccgcgt ttgacgcggc
    42901 agccgtaccg gtatccgaca gcgggtttgc cgccaatgca aatgtccgcc gttttgtgga
    42961 cgatgaagtc gggaaagggg atttttccca ggcggaatgg caggattttt ttgacaaagc
    43021 ggcttacaag gcggacatcg tcaagattat gcaccgaccc tccacatcgc gtccgtggta
    43081 tgtgttccgc acgggaaatt cgggcagggc gaaatttcac ggcgcgcgca ggttttatgc
    43141 ggaaaaccgc gcggttatcg atgatgtggc gcaaaaatac ggcgtgcctg ccgagcttat
    43201 cgtggcgatt atcgggattg aaacgaatta cggcaaaaat acgggcagct tccgtgtggc
    43261 ggacgcattg gcgaccttag gctttgatta tccccgccgc gccgggtttt tccaaaaaga
    43321 attggtcgag cttttaaagc tggcaaaaga agaaggcggc gatgttttcg cctttaaggg
    43381 cagctatgcg ggcgcaatgg gtatgccgca atttatgcct tcgagctacc ggaaatgggc
    43441 ggtggattat gacggggacg gacatcggga tatatggggc aacgtcggtg atgtcgcggc
    43501 atcggttgcc aattatatga agcagcacgg ttggcgcacg ggcggtaaaa tgttggtgtc
    43561 ggcgacgttg gcgccgggtg cggatgttca ggcaatcatt ggcgaaaaaa ccgccctgac
    43621 gcggacggtg gcggatttga aggcgtacgg catcatcccc ggggaaacgc tcgcagatga
    43681 tgaaaaggcg gttttgttca aactggaaac cgcacccggc gtgtttgaat attatttggg
    43741 cttgaacaat ttttatacgg tatggcagta caaccacagc cggatgtatg taacggcggt
    43801 cagggacatt gccaattcgc tcggcggccc gggattgtaa tgttttttaa aatgctgtct
    43861 gaaccacgtt tcggttcaga cggcattttt gcatcctttt atggggattt cccgcatcag
    43921 ggcggtattc ggggcgaatc ccgtttcaga cggcattgcc ggtcaaggct tgtccgaagg
    43981 aggatgtccc tgtcctttgg cgggcgggag gaaaagtatg caaggattgc ttttaatctg
    44041 attccaaaaa cttttgatca ggttgcggat gctgccgatt tttgtgccgc gcgagcgcag
    44101 ggcgacgaag agggcgaggc tgaagctgac gcagaggttg accaagccga tggcgaggac
    44161 gctgaaaatg ccgagtacga atgtaccaaa gccgacgttg ccgctgacgg cggcatagcc
    44221 gaggttggcg gaggaaaagg cgacgtggcg gatgtccagc ggcagcccga ggaggtgtcc
    44281 gaaatagccg gtcataccca agagcattcc gaagatgaag ttgccgacca gcgagccgta
    44341 gtgtttgtgg atgtaggcgg cgaggacgcg gcggggcccg gggcgcatga ttttacgcag
    44401 caagggattg aagggcaggc gttggcgcag gttgaggtag tcggcgcggt tgtcgaaaaa
    44461 acctgcgatg atgccggagc agaacagcca caagcctgca atagcggcat accatagggt
    44521 aggatgattg atgatgtcta tggatttgaa ctggtaggcg gcggtgtggg catcgagtat
    44581 gggcagccgg tacagatggg catagccgaa cgatatggcg cacgccaaaa ggatggcgat
    44641 ggaaacgttg ccgaagacgg cgacgctttg ggagcggcat acgtcgatga ggagcttgga
    44701 gagtttgttg tccaccgctt tgccgccttc gttgagatcg acctgttcgg caaagctggc
    44761 ggcagtcatc gcgggctgct tggtggcgac ggtgcagtgc agcatatgga tgatcataaa
    44821 gccgatgccg tagttgaacc cagccgacaa ggaagcgagg aagggactga ggtcgagtgt
    44881 gccgatgcgg attttgagca gcgccatcag ggcgatgatg ccgccgccgc ctgctgccga
    44941 gtagaacatt ttgaaatatt ctttgcggtt gcgggtgatg tagtgttcgc cgtggtggct
    45001 tttgttttcg gtaatgctgc gggcgagcat acggatgctg ctgcggcgca ggtggcgggt
    45061 gctgtattgt tcgaccgccg cgtagatgag ggagttcatc agggcgatgg tcaggcggtt
    45121 gctgttgccg gcaccggttt gtatgtctgt gagcagcttc agacggccta cggtctgccg
    45181 gagtcgttcg agcaggtggg cgactttgac cgacgaaccg gagccggcgc ccgtcccttt
    45241 gcgacgcaaa tagtcaatct ggctgaaaca ttggtcgaac atcacttcga ggtgggcggt
    45301 gtcgtaaggc gcggtgccgt tgcggtagtg ttcgaccagt ttggcggttt cgcgttggag
    45361 ggcgacgaag gaagaatcgg cttccagcag gcgcggggcg atacggatga gatcgggttc
    45421 gatggcttcg gatgccgtcc agacagacag catttcgatg gcgcgcaaac gcgcatcggc
    45481 aaggcggatg cccgccgtct gcaatttgga cggctcggcg tggctacgga tgagttcgta
    45541 taaaaccagc cattggcgga tattgagcgt ttgcagccat ttgtcgtcgt tttcggaatg
    45601 gaaaagataa aggaagactt cgcccaagtt ggcaaaatct ttatatgacg ggttgaagcg
    45661 ttcgtatatg cgtatgccca tttcccgggc aaagctgtgg cgcgagaaaa tgccgagttt
    45721 gatgagtgcc ggataaatgt gtatttgcgc gagccagatg taaaaacacc ggctgaagcg
    45781 ggacagtaat tccctgtctt gtttgaatgt gtcgataatc aggtcgaaac gctcccccgc
    45841 acattttttg ccgccacggc gcaaaaattt aatcaatgcg ttgaggacgt tgacaaaatc
    45901 ggtatgtccc aagctttccg aaagcagggg acgcaggttt tgaggggtaa ttttcttcat
    45961 atctgccatt gtaccgcatc ggcaatccgg cgttaaacgc cccgatggcg gggagggcgc
    46021 atctgcggcg ggcggcgcat tttccgccgg tttgaaatca aactatatta atgatataat
    46081 aaatatcgat aaatcaatct gatagaataa tccgtattga tttttaattt tttcgattat
    46141 aacaatttcg gcgggtctgt actag
//
LOCUS       DAWTCI010000005        43693 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWTCI010000005 DAWTCI010000000
VERSION     DAWTCI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 43693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43693
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.005"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(258..1043)
                     /locus_tag="WM481_000331"
     CDS             complement(258..1043)
                     /locus_tag="WM481_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFA9079734.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGFHGKATAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYSPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            1287..2102
                     /locus_tag="WM481_000332"
     CDS             1287..2102
                     /locus_tag="WM481_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9079735.1"
                     /translation="MTALKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHRLT
                     IVTPNLMATPMTENPPVFNPDDVQSFALPEESFILASGAADVLHNAEIVATLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVPELLQAANRGEKVALVFGNETFGLSIEEVRACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTDSPMTHLQQEDHAATHEQIKGMLAHME
                     SVMDDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            2143..3012
                     /locus_tag="WM481_000333"
     CDS             2143..3012
                     /locus_tag="WM481_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFA9079736.1"
                     /translation="MTDIRISMNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELS
                     VRELLGERGFRYLLALDADPAPLTDYLAQRAPFDHRLGIYAEELLAFWFTNAPHTKLH
                     AYNLTVFSDGQTLGAADFVVSLNQQPYHIELACKYYGGDQVQNLRGLNPKDTLTDKAA
                     KLVQQFQLVHTSQGKATLAAQDLPENPLPASIVRGIGFFPQGFHAFEPPLNPYGWRGI
                     YIQDWAEYGFERQEARYHLLDRMVYLAPARVAETETLNATEIRRIDQGLIAVLECRPD
                     GFWHEIERIMKAV"
     gene            3048..4304
                     /locus_tag="WM481_000334"
     CDS             3048..4304
                     /locus_tag="WM481_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFA9079737.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFRKHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFFG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWSEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESPDAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQGEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERIGSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGCLVYATCSVLPEENELQIKRFLSEHPEFEPVNCAELLQNLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(4362..4502)
                     /locus_tag="WM481_000335"
     CDS             complement(4362..4502)
                     /locus_tag="WM481_000335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079738.1"
                     /translation="MEYGYATVYFGKEKENCRQARIKSDEARDSAFGVESVESAVFDI
                     FN"
     gene            4650..5078
                     /locus_tag="WM481_000336"
     CDS             4650..5078
                     /locus_tag="WM481_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9079739.1"
                     /translation="MNFALSVIAFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTLQTKPAVKPKPAVDTNADSAKENEKDIAEKNGQLEEEKKKIAETERQ
                     NKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(5127..6470)
                     /locus_tag="WM481_000337"
     CDS             complement(5127..6470)
                     /locus_tag="WM481_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFA9079740.1"
                     /translation="MFHSVNVFTGETLYRRPDQDYAEFERRLADLKMHGRAFAQLGVT
                     ERAARLQKFAGRLEAEKERFAEMVCEEVGRCLHECRAEIVKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSRALFDLASDGIPLAGAWLDEADTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFIQLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKVPQGSGWFY
                     PATVLDRVNPACRVWREEVFGPVALILRAENEEHAICLANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(6475..7128)
                     /locus_tag="WM481_000338"
     CDS             complement(6475..7128)
                     /locus_tag="WM481_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFA9079741.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGALLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            7278..7568
                     /gene="gatC"
                     /locus_tag="WM481_000339"
     CDS             7278..7568
                     /gene="gatC"
                     /locus_tag="WM481_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFA9079742.1"
                     /translation="MALTLADVDKIARLSRLQLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMTHPHEAALRLREDEVTETDRAVEYQAVAPEVRNRLYIVPQVIEE"
     gene            7631..9076
                     /gene="gatA"
                     /locus_tag="WM481_000340"
     CDS             7631..9076
                     /gene="gatA"
                     /locus_tag="WM481_000340"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFA9079743.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACSSKMLDNFVSPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGMKIGLPKEYFSEGNSTDVQTA
                     LQHTIDLLKAQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSDGGLPIGV
                     QLIGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            9073..10005
                     /locus_tag="WM481_000341"
     CDS             9073..10005
                     /locus_tag="WM481_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFA9079744.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQDAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LAASVQGPSGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAALKLDGAIGIAQKHQV
                     KLRWKLKKRVKMNYDTFWQEYGASLERDGYWHLPQTPARKDLADIESKKRSMYRKRYE
                     MLDNMVAEMKDSLKTEARGISDGIQTEKPLRRTA"
     gene            10048..11478
                     /gene="gatB"
                     /locus_tag="WM481_000342"
     CDS             10048..11478
                     /gene="gatB"
                     /locus_tag="WM481_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFA9079745.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASAAFGSEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDARINQKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQEDFGTRREIKNLNS
                     FRFLDQAINYEAEAQIEILEDGGTVQQATMLFDPEKGETRVMRLKEDAHDYGYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRVQAAYFEEAAK
                     ESGQGKPTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSGKLAKKAF
                     EAMWAEPETSIAEIIEKHSLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKTSKGKANPAQVQELIKAKLA"
     gene            11570..12580
                     /locus_tag="WM481_000343"
     CDS             11570..12580
                     /locus_tag="WM481_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HFA9079746.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELASGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDININIPGCKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILPATDTGYAP
                     IRSILLDLIRQNSSRAAHFYWGARHQDDLYALEEAQGLACRLKNTCFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(12710..13342)
                     /gene="pdxH"
                     /locus_tag="WM481_000344"
     CDS             complement(12710..13342)
                     /gene="pdxH"
                     /locus_tag="WM481_000344"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFA9079747.1"
                     /translation="MDLHNIREDYSKRELSEADCADNPIEQFERWLDEAVRAEVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLELNPFAAMTFFWPELE
                     RQVRVEGRVGRLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            13402..13554
                     /locus_tag="WM481_000345"
     CDS             13402..13554
                     /locus_tag="WM481_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079748.1"
                     /translation="MHKLCPQSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAEE
                     PPYQAS"
     gene            13563..14612
                     /locus_tag="WM481_000346"
     CDS             13563..14612
                     /locus_tag="WM481_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HFA9079749.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETRKTAAQAY
                     GQKASDGIKLQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTTEQMRSLTEEGVMLEDGLAKVERIYEQGGEGANKWY
                     NIVIKEGRNREVRRIFESQGLTVSRLVRVAFGPIGLPNRLKRGQFYELNPAEVANILK
                     WADMLLPGERRRKKA"
     gene            complement(14671..16212)
                     /locus_tag="WM481_000347"
     CDS             complement(14671..16212)
                     /locus_tag="WM481_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFA9079750.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLFYLALMMFGAFTIRVPADGWK
                     PEGYTVPKTQNKPVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSAGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     SIAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGDGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(16471..17826)
                     /locus_tag="WM481_000348"
     CDS             complement(16471..17826)
                     /locus_tag="WM481_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFA9079751.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAQPLKEGDHIEVAGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPAAVQGAGSGFQIAQAIKTASQRAECDVLIVCRGGGSIEDLRAFNE
                     EPVVRAIEACTIPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLVQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMTQNLRA
                     HTARFERQTQALQHCRPDVSVYRQDIVRLQTALPAAFSRLLARRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFSDGETDVRVSKEQGQQDLFDC
                     I"
     gene            18023..18844
                     /gene="nadE"
                     /locus_tag="WM481_000349"
     CDS             18023..18844
                     /gene="nadE"
                     /locus_tag="WM481_000349"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFA9079752.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAAQANAKGFVVGVSGGI
                     DSAVVSTLAARTGRTTLLLDMPIRQHPGQLERARRHIRNLQGQYANVSAQTANLTDTF
                     QTFEQTVGVHRTAFANQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKVEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRRREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(18915..19388)
                     /locus_tag="WM481_000350"
     CDS             complement(18915..19388)
                     /locus_tag="WM481_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein DnrN"
                     /protein_id="HFA9079753.1"
                     /translation="MTDFSVWEAAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMRDELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTGNFHAPEGACGSWTRLYALAKEMADDLNDHIHLENDILFARVLDS"
     gene            19686..20018
                     /gene="trxA"
                     /locus_tag="WM481_000351"
     CDS             19686..20018
                     /gene="trxA"
                     /locus_tag="WM481_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFA9079754.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(20070..21017)
                     /locus_tag="WM481_000352"
     CDS             complement(20070..21017)
                     /locus_tag="WM481_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9079755.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPGEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVIAENGRKFWVNLDKYLDTGFFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDMERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMPGILDIQRDHQKLIDGAVNLLASDGILYFSNNLRSF
                     VLDDSVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            21345..22733
                     /locus_tag="WM481_000353"
     CDS             21345..22733
                     /locus_tag="WM481_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9079756.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGCDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLAPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGTSFGYQTRALSKPVDLIVATPGRLTDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPADRQTLLFSATWDGAVGKLARKLTKDSEIIEVERVDGQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLTDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPARKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDSFKKTGEGFKGKRKAGDS
                     FAGKSERRYKDR"
     gene            complement(22847..22953)
                     /locus_tag="WM481_000354"
                     /pseudo
     CDS             complement(22847..22953)
                     /locus_tag="WM481_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(23084..23284)
                     /locus_tag="WM481_000355"
     CDS             complement(23084..23284)
                     /locus_tag="WM481_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079757.1"
                     /translation="MPSEGLSDGIFVFVRGIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVIPPLGFQTASYRTVKP"
     gene            complement(23298..23852)
                     /locus_tag="WM481_000356"
     CDS             complement(23298..23852)
                     /locus_tag="WM481_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079758.1"
                     /translation="MKKIIASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAQAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLIGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPVNYRRPAMRGFGRRR"
     gene            complement(23937..24437)
                     /locus_tag="WM481_000357"
     CDS             complement(23937..24437)
                     /locus_tag="WM481_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079759.1"
                     /translation="MDAMPQTAVQNENLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANKRACRFAEKHRLFFTPAQAELSLNKHKNRRLLTIETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            24840..26033
                     /locus_tag="WM481_000358"
     CDS             24840..26033
                     /locus_tag="WM481_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFA9079760.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVKHSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQAARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCAL
                     DTPYRGRSSEIAATSLKHGVMILVAGADVLRFAPSLLLNDEDIAEGLRRLEHVLTEFA
                     AANRP"
     gene            complement(26140..27384)
                     /gene="clpX"
                     /locus_tag="WM481_000359"
     CDS             complement(26140..27384)
                     /gene="clpX"
                     /locus_tag="WM481_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFA9079761.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCLEILYEG
                     DNGGTPPENAGGEPEESGKLPTPAEIVANLDDYVIGQGQAKKALAVAVYNHYKRLRHP
                     KADGGVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQHGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTANILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENAGITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGIENVGLEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            27567..27938
                     /gene="rbfA"
                     /locus_tag="WM481_000360"
     CDS             27567..27938
                     /gene="rbfA"
                     /locus_tag="WM481_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFA9079762.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITINEVE
                     VTRDYSHATVFYTVLNQDTREITEEVLEHARGHLRSELSKRIKLFKIPELHFKYDESL
                     ERGMSLSALIDQVAAEKPVED"
     gene            27949..28473
                     /locus_tag="WM481_000361"
     CDS             27949..28473
                     /locus_tag="WM481_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079763.1"
                     /translation="MNNLIPEHLAAYAHSDNLQIEGGHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSQNQKQNRTLTDL
                     DEYTYRVPIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISIDLID
                     EKHSVRELLNEELS"
     gene            28531..29451
                     /gene="truB"
                     /locus_tag="WM481_000362"
     CDS             28531..29451
                     /gene="truB"
                     /locus_tag="WM481_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFA9079764.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEAAKFAQYLIDADKAYTATLKLGEASSTGDAEGEIIAAARADI
                     SLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYSIDIA
                     EFDAPKAVISVRCSKGTYIRTLSEGIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDGLLLPCDVLVSHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRMKALRLMNTAASSA"
     gene            29669..31855
                     /locus_tag="WM481_000363"
     CDS             29669..31855
                     /locus_tag="WM481_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9079765.1"
                     /translation="MKTGIQTELAQALLSHEKIWANEEKTILAKNILLDLVEKTDPSI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSINNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRNNSQPASQPASQPASQPASQPASQL
                     YTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRHTADGKQPVGEIKRRSDGTPAE
                     NLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYNTETDSFAYNDKFSHSTWLTFMKNRL
                     EIAKELLKDDGLIFVQCDDKEQAYLKVLLDETFTRENFINCIAVKMSEPSGNKMAHTS
                     HRLPKIKEYILIYKNKNIKLNPIREQKSEWDNEYNIFLENFTQEDKKFIDLIVNSQTE
                     NKEINGNTLKEIDILLKKISPISVNQKLAQLNIKDNEVIKWKLDNAYRIVRTAASSSV
                     KKLADEKKEICQQQFFSVISKRDKLLYIVKSDYSKDAKAPRVQVLFAEDYLSISLCDL
                     WTNINTTGLEAEGNVELKNGKKPESLIETIIKLATNENDIVLDYHLGSGTTAAVAHKM
                     NRQYIGIEQMDYIETLAVERMKKVIDGEQGGISKAVNWQGGGEFVYAELSPFNETAKQ
                     QILACENSDGIKTLFEGLCERHFLKYNVSVNEFSQIIEEPEFQSLALDEQKQMMLEML
                     DLNQMYISLSEMDDEQFAGCLNDDDKALSRAFYQSVKHQAEKKDGE"
     gene            31845..34619
                     /locus_tag="WM481_000364"
     CDS             31845..34619
                     /locus_tag="WM481_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFA9079766.1"
                     /translation="MANNKTLFDWVEDRKSMLEEMEQTDFFALPEFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMIAALILYYFEKGYRH
                     FLFFVNKNNIVYKTENNFIDPTHPKFLFTEKILQGDTVIPIRKVETFSQYSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEKEMNE
                     RTSDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENTEVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSGTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWVENVQADDFSFLTTFSTSLNDSNNANEQGKTRTEQALKFMQENK
                     FEFVHLANWIKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQVHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLD
                     IQNRNELQNKLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDIPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDIKTAIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFVLLLKDKQKSSSDSVDDFLHYQIFI
                     EPKGGHLVENDSWKEAFLKSITVEYGRDKILQKNTPHYRLIGLPFFTDHQKNGQFTEL
                     FPLGAASLEK"
     gene            complement(34768..35940)
                     /locus_tag="WM481_000365"
     CDS             complement(34768..35940)
                     /locus_tag="WM481_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFA9079767.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGGDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTAEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDVSMAFTGHRDIQDVDASILRSKDWGRETV"
     gene            complement(36061..36291)
                     /locus_tag="WM481_000366"
     CDS             complement(36061..36291)
                     /locus_tag="WM481_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079768.1"
                     /translation="MVVVARVGFFIVGGIVQYLSVSVKYGGAVADCLFKWRYSLILLY
                     LFGFLNQLVMFFEERRLGRFAGKRFQNDLIYF"
     gene            36290..36736
                     /gene="iscR"
                     /locus_tag="WM481_000367"
     CDS             36290..36736
                     /gene="iscR"
                     /locus_tag="WM481_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFA9079769.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPPAQINIAQIISAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            36765..37979
                     /locus_tag="WM481_000368"
     CDS             36765..37979
                     /locus_tag="WM481_000368"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFA9079770.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WTAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISIMWVNNEIGVV
                     QNIPAIGEICRERKIAFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELAQDTAHYLKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            37964..38275
                     /locus_tag="WM481_000369"
     CDS             37964..38275
                     /locus_tag="WM481_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079771.1"
                     /translation="MGGALRLMSNKGCLRKNTMKVKIINLPNGYKRIIYGKYFEQFDL
                     DYEQDLDVLKKDIEFALSVIEYNRSIFKKFSSLFENKIIFVYQGGHHLDIIDRDKGSL
                     K"
     gene            38608..39075
                     /locus_tag="WM481_000370"
     CDS             38608..39075
                     /locus_tag="WM481_000370"
                     /inference="COORDINATES: protein motif:HMM:NF014691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9079772.1"
                     /translation="MAHLQTIHRELFGNVYDWAGKIRRVDISKGNTRFANFAFIENES
                     RKLLEKLKNENYLRVLDKDKFAERAAYYLDELNVLHPFREGNGRTLRLFMTQLAIKNG
                     FQIHWQNISAEQMIQACIQAYHADSSLLARLIIRQFRTIVFFRRLKHQQTTIF"
     gene            39088..39474
                     /gene="iscU"
                     /locus_tag="WM481_000371"
     CDS             39088..39474
                     /gene="iscU"
                     /locus_tag="WM481_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFA9079773.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENK"
     gene            39561..39881
                     /gene="iscA"
                     /locus_tag="WM481_000372"
     CDS             39561..39881
                     /gene="iscA"
                     /locus_tag="WM481_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFA9079774.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEANGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEDLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(39841..40062)
                     /locus_tag="WM481_000373"
     CDS             complement(39841..40062)
                     /locus_tag="WM481_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFA9079775.1"
                     /translation="MGGKAQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKRRDGFDTVPPFLCLKRGSSRRSRRSL"
     gene            40144..40644
                     /gene="hscB"
                     /locus_tag="WM481_000374"
     CDS             40144..40644
                     /gene="hscB"
                     /locus_tag="WM481_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFA9079776.1"
                     /translation="MSQYFTLFRIEPAFDIGTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFAPDFLMQQMEWRET
                     LMEARAGNNLESLKNLDNEIRAEQEKLFCGLKQSFARQDCDTAAQQVRQGRFLDKLRH
                     EISSAL"
     gene            40742..43492
                     /gene="gyrA"
                     /locus_tag="WM481_000375"
     CDS             40742..43492
                     /gene="gyrA"
                     /locus_tag="WM481_000375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFA9079777.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDFAVYATIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLTDTINACLRLLDEPKTEIDELID
                     IIQAPDFPTGATIYGLGGVREGYKTGRGRVVMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKKQGYYLSEIQADAILRMSLRNLTGLDQKEIIESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     DLEDETADNENTLPSGKNGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGSIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSGASVISNVTEPEA
                     EN"
BASE COUNT        11633 a        11959 c        10594 g         9507 t
ORIGIN      
        1 attccccggt gtctgttttc tgccacagtc gatttagatt ccagcaaacc gcctttaaac
       61 aattgaaaga aaagggacaa attgcacagg gttctccgtt taaccagata cggatttcca
      121 ttcccggcaa tttcgggcat ttgaccgttt ccgccttcct gccccgccgc caataaaaat
      181 gccgtctgaa atatcggcac gatacttcag acggcatacc cgcccgtttt cccgcatttc
      241 cgggagctgg ctgaaattta aacgtgtgcg gaaatgattt ttaacatttg cgccagtact
      301 ttgggattgg cagccacaat atcgccgctt tccaaccaag cgtcctcacc cgacatatcg
      361 gtaacgatgc cgcctgcttc ttggacaatc aatgcaccgg cggcaatgtc ccacggtttg
      421 aggttgaact cgaaaaagcc gtcaaaacgt cctgttgcta cggcgcacaa atccaaagaa
      481 gccgcgcctt cgcggcggcc tccggcggtt tttgccaaaa agtctttcaa aatcgccaga
      541 tatttgtcca tcatgctttg atcgacaaca gggaaaccgg taccgatcag gcagcggttc
      601 aattcgatgc ggttggagac acggatgcgg cggtcgttaa gcaacgcgcc tttaccgcgc
      661 gaagccatgt acacgtcgtt acgttcgggg gagtacacca aagcttcctg caacacgcct
      721 ttgtgcagca gtgccataga gatggcgtat tggggatggc cgtgaaggaa attggtcgtc
      781 ccgtcgagcg gatcgataat ccattcgtac tcggctgtag ctttgccgtg gaagccgctt
      841 tcttcgcaag tgattttgtg atgcggatag gcttctttca aagcctcaac caggatgatt
      901 tcggaattgc ggtcaacatc ggaaacaaaa tcgttgaagg ctttgctgtc ggttttgacg
      961 gcatcgaggt tacctgcggc gcgaatcatc atctgaccgg cacggcgggc ggctttaaag
     1021 gctgtattca aaaacggatt catcagattt ccttaagggt ggcataccgc cggttcggac
     1081 gtgcagtcct tcggagcggc aaaatcggag tttatctggt tggggcaaac cctgccaaat
     1141 cgggtaaaat accgcctgac gcgtgtctgc ttcaggcgca acgttaaatt tccgacgttg
     1201 ttaaagaaca gttcaaacgg catttcaccg tccgaacgaa aaagacggcg cattataccc
     1261 tattccattc cgaccgaaaa ccgaacatga ctgctctcaa acccgccctg cccgactacc
     1321 tcggcaacat ccgcatcatc ctcacacgca ccagccaccc cgccaacatc ggctctgccg
     1381 cacgcgcgat gaaaacgatg ggtctgcaca ggctgaccat cgtcaccccc aatctgatgg
     1441 caacgccgat gacggaaaat ccgcccgttt ttaatcccga tgacgtgcaa agttttgcac
     1501 tgcctgaaga aagtttcatc ctcgcttccg gcgcggcgga cgtattgcac aacgccgaaa
     1561 tcgtcgccac actggacgaa gcccttgccg acaccaccat cgcctgcgcc ctgaccagcc
     1621 gccgccgcga aattaccgcg ccgctgcaaa ccccgcgcga tttggtgccc gaattactgc
     1681 aggccgccaa ccgcggcgag aaagtggcgc tggtcttcgg caacgagact ttcggcttga
     1741 gcatcgaaga agtccgagcg tgcaaccgac tgatgaccat caacggcaac cccgactatt
     1801 tctcgctcaa cctcgcccaa gccgtgcagg tcgtgtgcta cgaaattttc agccaaaccg
     1861 attcgcccat gacccatctt caacaggaag accacgccgc aacccacgag caaatcaaag
     1921 gcatgctcgc ccacatggaa agcgtgatgg acgacatcgg ctttttcaac cgccgcaacg
     1981 gcgagcgtct gatgcgccgt atgcagagcc tgttcggacg cgccaacacg caaaccgaag
     2041 acatcgacat cctgcgcggt tttttcaata ccgtcagcca ccgtatccat aaaaaagact
     2101 gattaaggcc gtctgaaaac attacaagct ttttcagacg gcatgactga tattcggata
     2161 agcatgaatt acgccctaga cgcattatgg tggaaactca ccagccaacc cgtccgcgac
     2221 ctcgcctcgc tgctgactgc gccgcctttg tggcaaagcg gttgcgaatt gagcgtgcgc
     2281 gaactactgg gggaacgcgg tttccgctac cttttagcat tggatgccga tcccgcgccg
     2341 ctgacggatt accttgcaca acgcgccccg ttcgaccacc gtctcggcat ttatgccgaa
     2401 gagctgctgg ctttttggtt taccaacgca ccgcacacca aactgcacgc atataatctg
     2461 accgtttttt cagacggcca aactttaggc gccgcggatt ttgtcgtttc cctcaaccaa
     2521 cagccctatc atatcgagct ggcgtgtaaa tactacggcg gagaccaagt gcagaacctg
     2581 cgcggtctca atcccaaaga cacgctgacg gacaaagccg ccaaattggt gcagcaattc
     2641 cagctggtgc atacgtcgca aggcaaagca accttagccg cacaagacct gccggaaaat
     2701 ccacttcctg cttccatcgt gcgcggcatc ggattttttc cacaaggttt ccatgctttt
     2761 gagccgccgc ttaatccata cggctggcgc ggcatctata ttcaagattg ggcggaatac
     2821 gggtttgaac gccaagaagc gcgctatcac ctgctcgacc gcatggtcta tctcgcgcct
     2881 gcgcgtgtcg ccgaaaccga aacattaaac gcaaccgaaa tccgccgcat cgaccaaggc
     2941 ttgattgccg ttttggaatg tcggccggac ggcttttggc acgaaatcga acgtattatg
     3001 aaggccgtct gaaacccttt accaacttta agagaaccca ttacattatg aacgccgcac
     3061 aactcgacca taccgccaaa gttttggctg aaatgctgac tttcaaacag cctgccgatg
     3121 ccgtcctctc cgcctatttc cgcaaacaca aaaagctcgg ccgccaagac cgccacgaaa
     3181 tcgccgaaac cgcctttgcc gcgttgcgac actatcaaaa aatcagtacc gccctgcgcc
     3241 gtccgcacgc gcagccgcgc aaagccgcac tcgccgcgct ggttctcggc agaagtacca
     3301 acatcagcca aatcaaagac ctgcttgatg aagaagaaac agagtttttc ggcaatttga
     3361 aagcccgtaa aaccgagttt tcagacggcc tgaataccgc cgcagaattg ccgcaatggc
     3421 tggtggaaca actgcaacag cattggagcg aagaagaaat cctcgctttc ggccgcagca
     3481 tcaaccaagc tgcgccgctc gacatccgcg tcaatacctt gaaaggcaaa cgcgacaaag
     3541 tattgccatt gttgcaagcc gaaagccccg atgcagaggc aacgccttat tctccttggg
     3601 gcatccgcct gaaaaacaaa atcgcgctta acaaacacga actgtttttg gacggcacgc
     3661 tggaagtcca aggcgaaggc agccagctgc ttgccttatt ggtgggcgca aaacgcggcg
     3721 aaatcattgt ggatttctgt gccggagccg gcggtaaaac cttggctgtc ggcgcccaaa
     3781 tggccaacaa aggcagaatc tacgccttcg acatcgccga aaaacgcctt gccaacctca
     3841 aaccgcgcat gacccgtgcc ggactgacca acatccaccc cgaacgtatc ggcagcgaac
     3901 acgatacccg tatcgcacga ctggcaggca aagccgaccg cgtgttggtc gatgcgccct
     3961 gctccggctt aggcacactg cgccgcaatc ccgacctcaa ataccgccaa tccgccgaga
     4021 ccgtcgccaa acttttggaa cagcaacaca gtatcctcga tgccgcctcc aaactggtga
     4081 aaccgcaagg ctgtttggtt tacgccacct gcagcgtgtt gccggaagaa aacgaactgc
     4141 aaatcaaacg tttcctgtcc gaacatcccg aatttgaacc cgtcaactgc gccgaactgc
     4201 tgcaaaacct gaaggtcgat ttggataccg gcaaatacct gcgcctcgat tccgcccgac
     4261 accaaaccga cggcttcttc gccgccgtat tgcaacgcaa ataaaccggt ttgaacaaaa
     4321 tgccgtcaga acccttttca aagcgttcgg acggcatttc atcaattaaa aatatcaaaa
     4381 accgcagatt caaccgattc aacaccaaag gcagaatcac gcgcctcatc ggattttata
     4441 cgcgcctgcc tgcaattttc tttttctttg ccaaaatata cggtagcata accatattcc
     4501 aatgatattt ttgggaatct gttttacccc aatatataaa gcaccatatt aaggcggagt
     4561 gtcttccctg ctttgacccg aacccggaaa agacaccgcc cgaaccaatc ccgattctgc
     4621 ccgggcagtc agccatcaag gaaaccctaa tgaactttgc tttatccgtc atcgcattta
     4681 ccctcgcctc tttcctgccc gtcccgcctg ccggagccgc cgtctttact tggaaagacg
     4741 gcggcggcaa cagctattcg gatgtgccga aacagcttca tcccgaccag agccaaatcc
     4801 tcaacctgcg gacgctccaa accaaaccgg cggtcaagcc caaacctgcc gtcgatacga
     4861 atgcggacag tgcgaaggaa aacgaaaagg atatcgccga gaaaaacggg cagcttgagg
     4921 aagaaaagaa aaaaattgcc gaaaccgaac ggcagaacaa agaagaaaac tgccggattt
     4981 caaaaatgaa cctgaaggcg gtgggaaact caaatgcgaa aaacaaggat gatttgatcc
     5041 gtaaatacaa taacgccgta aacaaatact gccgttaatc ggctctagcg caaacccgat
     5101 gccgcctgaa gcggcacggg gtttgtttat ttctgccagt aggttttgac gttgacgaac
     5161 tcgtacaacc cgaattcgga cagttcgcgc ccgtaaccgg aatctttgac tccgccgaaa
     5221 ggcaggcgca aatcgctgct ggtatggcgg ttgataaaca ccgatccggc ctgtattttt
     5281 tcggcaaacc gccaggcgcg ttcagtatcg gcggtataaa tgcaggcacc gagcccgaac
     5341 ggggaatcat tggcaaggca gatggcgtgt tcttcgtttt cggcgcgcaa aatcagggcg
     5401 acggggccga atacttcttc tctccagacg cggcaggcag gatttaccct gtctaaaacc
     5461 gtcgcagggt aaaaccaccc cgacccctgt ggaactttgc ctccggtcag gcacaccgcg
     5521 ccgtttgaaa cggcatcttc aacctgcccg tgaacccggt cccgcaaatc ttcgcggtgc
     5581 agcggtgcaa gcgtggtatc gggatgtttc gggtcgccca ttttcaattt ggcgcattcg
     5641 gcgagaaaca gctgaataaa gcggtcggct gcggcttcgg ttacgatgat gcgcttggcg
     5701 gcgttacacg attgccccgc atcgcggaaa cgggaataac aggcttctgc ggcggcgcgt
     5761 tccaaatcgg catcgggcat cacgataaag gcgttgctgc cgccgagctc caacacggtt
     5821 ttcttgaggt ttgcgcccgc atgtgccgca agaatgcgcc ccgtatgcgt cgaaccggta
     5881 aacgccatcg catcggtatc ttcgactgcc ttgagcgtgt ccgcctcatc cagccacgcg
     5941 cctgccagag gaataccgtc tgaagccaaa tcgaacaacg cccgactgac gcgtgccacg
     6001 ctgggcgcgg gtttgacggc gcacgcgttg cccgcgcaca tagcgggaac ggcaaaacgc
     6061 aatacctgcc agacgggata gttccaaggc atgacggcaa acaccacgcc caaaggctcg
     6121 aagcgcacct gactcaaact cgcctgcgtc gcaatggttt tgtgggcgag cagttcgggg
     6181 gcgaggcggg cgtaatagcg tatcagttcg atagacttga cgatttcggc acggcattcg
     6241 tgcaggcagc gtccgacttc ttcgcacacc atttccgcaa aacgctcttt ttccgcctcc
     6301 aaacggccgg caaatttttg cagacgcgcg gcacgttcgg ttacgcccaa ttgcgcgaac
     6361 gccctgccgt gcattttcaa atccgccagc cgccgttcaa actccgcata atcttgatcg
     6421 gggcggcggt aaagcgtttc gcccgtaaat acattgacac tgtgaaacat cgaatcaacc
     6481 tgccagttgc gggaatatcg ttttcagtcc cgacacaata atctccaccg ataccgccgc
     6541 cagcatcata cccataatgc ggtttaaaat cgtcagcccc gtcgcgccca gcaggcggct
     6601 gaccttcccg gcaacgatta aaatggcata acaaatcgca ctgaccacca aaccggccgc
     6661 gataatcagc gcgatatcgc tgtacgtttt ggctgccgaa gcgtaaataa tcacggtcga
     6721 aataccgccc ggaccgatgg tgatcggtat ggcgatgggc acgacggcaa tcgcccctgc
     6781 attgcgggcg gggcgcgctt gccccgtttc cggctgcgcg ccgagattct gcttggcggg
     6841 attgtcgttg ccgttcatca tcgaaatggc gatcagcagc accaaaatcc cgccgccgac
     6901 ctgaaacgaa ccgacgctga tgcccaaaac cttcaatagc gcaccgccga tcagcgcaaa
     6961 taccgcaatc acggcaaaca cggcgacggc ggccgtccgc gcgaccttcc tgcgctcctt
     7021 cgtgctgtgt ccgttggtca ggtcaaggta aagcgacaac gcgctaaacg gattgatcag
     7081 caccaaaaaa gccacaatca gcttgccgat ttccataccc aatcccatta tttcccctct
     7141 ttcaaacccg tgcggcaggc atccgatgct gcaaattgcc gccgcaacgg atttttccgt
     7201 tataattaaa aatccaagca atacgcccca tcatacccga acgacggtat ctttaccatc
     7261 agacaaggat gcttttcatg gcactgacac ttgccgacgt agacaaaatc gcccgactct
     7321 cccgactgca gctgactgcg gcagaaaaag aaaaatcgct tcaagaatta aacgacattt
     7381 tcgctatggt cgaacagatg caaaccatta acacagacgg catcgaaccg atgactcacc
     7441 cgcacgaggc cgccctgcgc ctgcgcgaag acgaagtaac cgaaaccgac cgcgccgtcg
     7501 aatatcaggc ggtcgcaccg gaagtacgca accgtctgta catcgtaccg caagttatcg
     7561 aagaataatc cgaatatgct tcagacggca tcagcaatac cgcctgaaac cctttaggga
     7621 tggaagattt atgacacaat acacattgaa acaggcaagc gtcctgttgc agtccaaaca
     7681 gatttccgcc gtcgaactag caagcgcata ccttgccgcc atcgccgaaa aaaatcctgc
     7741 cctcaacggc tacatcacca tcgaccaaga taaaaccctc gcagaagccc gtgccgccga
     7801 tgaacgcatc gcgcagggca acgcctccgc gcttaccggc gttcccgtcg cctacaagga
     7861 tattttctgc caaaccggct ggcgcagcgc gtgcagctcc aaaatgctgg acaacttcgt
     7921 ctccccctac acagccaccg tcgtccaaaa cctgctcgac gaaggcatgg taacgctcgg
     7981 ccgcaccaat atggacgagt tcgctatggg ttcgaccaat gaaaactcat tctacggcgc
     8041 agccaaaaac ccgtggaatc ttgagcacgt acccggcggt tcgtccggcg gttccgccgc
     8101 cgtcgttgcc gcgcgcctcg cccctgccgc gctcggttcg gataccggcg gctctatccg
     8161 ccaacccgcg tcgcactgcg gcattaccgg catcaaacct acctacggca cggtttcccg
     8221 cttcggcatg gtcgcctacg cctccagctt cgaccaggcc ggcccgatgg cgcaaactgc
     8281 cgaagactgc gcgattctgt taaacgcgat ggcaggtttc gaccccaaag actccaccag
     8341 cctcgagcgc gaaaaagaag actacacccg cgatttgaac caaccgctta aaggcatgaa
     8401 aatcggcctg cccaaagaat acttcagcga aggcaacagc accgatgttc agacggcatt
     8461 gcaacatacc attgacctcc tcaaagccca aggcgcggaa ttggtcgaag tttccctgcc
     8521 gcaaaccaag ctgtccatcc ccgcctacta cgtcctcgcc tccgcagaag ccagtaccaa
     8581 cctttcacgt tacgacggcg tacgttacgg acaccgtgcc gcccaattcg gcgatttgga
     8641 agaaatgtac ggcaaaaccc gcgccgaagg tttcggcagc gaagtcaaac gccgcatcat
     8701 gatcggcact tacgtactat cgcacggcta ctacgatgcc tactatctca aagcccaaaa
     8761 actgcgccgc ctcgttgccg atgactttca gacggcattt gcacggtgcg acctcatcct
     8821 cgcgccgacc gcaccctccg ctgcacccaa aatcggagcg gatgcttcgc cggttgaaac
     8881 ctacttgagc gacatttaca ccatcgccgt caacctcgcc ggactgcccg cattgaccct
     8941 gcccgcaggc ttcagcgacg gcggactacc catcggtgta cagcttatcg gcaactactt
     9001 cgccgaagcc aaaatcctcg gtgcggcgca tcaaatccaa ctcaatagcg attggcacgg
     9061 caaacgaccc gaatgaagca gaaccgcacc tttaccttcc ccgattttcg caccgtttac
     9121 agctatgccc ctttatatcg gctgcaacat ttaaaataca cattgcgaaa atttttcgga
     9181 aaaaaagaaa tttacgcctt cgagcagttt gtcaacgcct cccctatccg tcaggggctg
     9241 ttcctccact gcccgcagga tgcctatccg ctgctgcgcg aatttgttga caggcgtttt
     9301 aactgcaaac gccgtttaga tgcgatgacg gcagattttc ttatggcgga aaaactgttc
     9361 ggcacagaca ttctgcacca aatggaagac taccgcttcc atctggtctt ggcgcacctt
     9421 tcagacggca tcagcttgtg gctcaaccgc aacgacaact gcgtcgaaga aggcgcgtgg
     9481 tctttatctt tgcgcgatga agcaggcaac cggctgtata tggcgacctt cgcctttgtc
     9541 ggcacacacc tgctggcagc ctccgtacaa gggccttcgg gcgaagaggc caaagacacc
     9601 gtccgccgca tcaccaaaca acttcacggc ctgcgtcccc aacaactgat ggtaaccgca
     9661 ctgcaatatt tcgccgccgc attaaaatta gacggcgcga taggcattgc acaaaaacat
     9721 caggtcaaac tgcgttggaa acttaaaaag cgcgtcaaaa tgaattacga cacattctgg
     9781 caggaatacg gcgcaagttt ggaacgggac ggctactggc atctccctca aactcccgcc
     9841 cgcaaagacc ttgccgacat cgaaagcaaa aaacgctcga tgtaccgcaa gcgttatgaa
     9901 atgctggaca acatggttgc agagatgaag gacagtctga aaacagaagc acgcggcatt
     9961 tcagacggca tccaaacgga aaaaccgctc cgccggacag cctgacgaga agactatcga
    10021 attgatattt tagagaaaga acctcctatg acctgggaaa ccgtaatcgg cttggaaatc
    10081 cacgtccaat tgaacaccaa atccaaaatc ttcagcggcg catcggccgc attcggctca
    10141 gaacccaacg cgcacgccag cgtagtggag tgcgcgctgc cgggcgtact gccggtaatg
    10201 aaccgggaag tcgttgaaaa agccatcaaa ttgggtttgg ccttagatgc gagaatcaat
    10261 cagaaaaacg tgttcgaccg caaaaactac ttctatcccg acttgcccaa aggctatcaa
    10321 atcagtcagt tggacttacc gattgtcgaa cacggcaaat tggaaatcgt agtcggcgac
    10381 gatgtgaaaa ccatcaacgt aacccgtgcc catatggaag aagacgcagg caagtccgtg
    10441 catgaaggct tgaacggcgc gaccggtatc gacctgaacc gcgccggcac gccgctgttg
    10501 gaagtggtat ccgaacctga aatgcgctcc gccgccgaag ccgttgccta cgccaaagcc
    10561 ttacacagct tggtaacctg gctagacatt tgcgacggca atatggcgga aggttcattc
    10621 cgcgtcgatg ccaacgtatc cgtgcgcccg aaaggtcaag aagatttcgg cacgcgccgc
    10681 gagattaaaa acctcaattc cttccgtttc ttggatcagg cgattaatta cgaagccgaa
    10741 gcgcaaatcg agattttgga agacggcggc acagttcagc aggcgacgat gctgttcgac
    10801 ccggaaaaag gcgaaacccg cgtgatgcgc ctgaaagaag acgcgcacga ctacggctac
    10861 ttccccgatc ccgatttgct gcccgtcatc atttcagatg cccaaatgca aaaagccaaa
    10921 gcagaaatgc ccgagctgcc gaaagaaatg gcggcgcgtt tcgtggcgga ttacggcgtg
    10981 tccgaatacg acgcgcgcct gctcaccgcc agccgcgtgc aggctgccta ttttgaagaa
    11041 gccgccaaag agagcggaca aggcaagccg actgccaact ggatgaacgg cgaacttgcc
    11101 gctacgctga acaaagaagg catggaactt gccgacagcc cgattaccgc cccacgcctt
    11161 gccgcgctgg tgggcaaaat tgccgatggc acattaagcg gcaaactggc gaaaaaagcc
    11221 tttgaagcca tgtgggcgga accggaaacc agcattgccg aaatcatcga aaaacacagt
    11281 ttgcaacaga tgaccgatac cggcgcggtt gaagcgatgg tggacgaagt gctggcaaac
    11341 aacgccaaag ccgtggaaca gtttaaatcc ggcaacgaaa aagccctgaa tgcgattgtg
    11401 gggcaagtga tgaagaccag caaaggcaaa gccaatcccg cgcaggtgca ggagttgatt
    11461 aaagccaaac ttgcctgata agcaagatgc cgtctgaaac ccaaatccga cgttcagacg
    11521 gcattttcac agtctgacca cattaaaaca gcataaaaaa caatattcca tgaaccacac
    11581 cgttaccctg cccgaccaaa ccacctttgc cgccggcgac ggcgaaaccg ttttgtccgc
    11641 tgccgcccgt caaaacctca atctgcccca ttcctgcaaa aacggtgtct gcggacaatg
    11701 caaagccgaa ctggccagcg gcgatattca aatgggcgga cactcggaac aggctttatc
    11761 cgaagcagaa aaagcgcaag gcaagatttt gatgtgccgc accaccgcgc aaagcgatat
    11821 caacatcaac atccccggct gcaaagccga tgccctaccc gtccgcaccc tgcccgcacg
    11881 catcgaaagt atggttttca aacacgatgt cgccctcctg aaacttgccc tgcccaaagc
    11941 cccgccgttt gccttctacg ccgggcaata cattgattta ctgctgccgg gcaacgtcag
    12001 ccgcagctac tccatcgcca attcgcccga ccaagaaggc attttggaac tacacatccg
    12061 caggcgcgaa aacggtgtct gctcggaaat gattttcggc agcgaaccca aagtcaaaga
    12121 aaaaggcatc gtccgcgtta aaggcccgct cggttcgttt accttgcagg aagacagcgg
    12181 caaacccgtc atcctgccgg caaccgacac aggctacgcc cccatccgca gcatcctgct
    12241 cgaccttatc cgccaaaaca gcagccgcgc tgcccatttc tactggggcg cgcgtcatca
    12301 agatgatttg tatgccctcg aagaagcaca agggttggca tgccgtctga aaaacacctg
    12361 cttcaccccc gtattgtccc gtcccggaga gggctggcag ggaagaaaag gccacgtaca
    12421 agacatcgcg gcacaagacc accccgacct gtcggaatac gaagtgtttg cctgcggctc
    12481 tccggctatg accgaacaag caaaaaatct gtttgtgcaa cagcataagc tgccggaaaa
    12541 cttgtttttc tccgacgcat tcacgccgtc cgcatcataa ttcctcggta taaagaggat
    12601 tcgggctttc cgttcggaac acaaaaaact tcccgtccgt attttccccg tgaaaaaatg
    12661 ccgtctgaaa cctgattccg gttttcagac ggcatatgtt ttttcctgtt caaggcgaca
    12721 accgctcgcg tatccagccg ccatccagca aacggtattg gatgcggtcg tgcagcctgc
    12781 tcggcctgcc ctgccaaaac tcaatcaaat cgggaatcac aatatagccg ccccaatgcg
    12841 gcggacgcgg cacgtgcaaa ggatgtttga gtccgaccgc cgccgccttt gccaccaata
    12901 ccgccttgtt cggaatcacc tcgctctgcg cgcttgccca cgcacccaaa cggctctgat
    12961 acgggcgact ctcaaaatat tcgtccgaca acttctccgc cagccttccg acacgccctt
    13021 ccacgcgcac ctgacgctcc agctccggcc aaaaaaacgt catcgccgca aacggattga
    13081 gttccagcga acgccccttg cggctgtggt aattcgtaaa gaaaacaaaa ccttctgaat
    13141 taacctcctt caaaagcacc atacggctgt tgggcctccc gcgcccgtcg accgccgcca
    13201 cattgaccgc cgtcggctcg ttgacctcgg cgcgtaccgc ctcgtccaac caccgctcga
    13261 actgctcgat cggattatcg gcgcaatcgg cttccgacaa ttcccgtttg ctgtaatctt
    13321 cccgaatatt gtgcaaatcc atttaccgct cctcttatca tttgaaagat tctactcccg
    13381 cacgcaaacc gatttcaacc gttgcacaaa ctttgtcccc aaagccgcag cgacgatttc
    13441 atccgcaaaa ccgccgcatc aggtacaata tcgaaccgtc cgaccgagga cggcatttta
    13501 tcaacccgtc ctgccgcaca cgccgcagaa gaaccgcctt atcaggcgag ttaggaaaaa
    13561 tgatgtccaa gcaacccacc agcaaacgcc aatggcgcga cggcgcagcc ccgtctgcca
    13621 agaaaaccgc caaacctttc aaaagcaaag cccgtcccaa agacgaaacg cgcaaaaccg
    13681 ccgcccaagc ttacggacaa aaagcttcag acggcatcaa gctgcaaaac gcccccaaac
    13741 agcgcgccgc caaagccaaa aaactcgtcg tccgcaatcc caaccaaaaa attatggaac
    13801 acgcgcgcga tttgaaagaa cgccgcagcg acctgtcgcg tatggaaccc gaacgcctgc
    13861 aaaaagtgct tgcagcctcc ggcgtcggct cgcgccgcga aatggaagaa tggatcaaca
    13921 acggctgggt aacggtcaac ggcaaaaccg cgcaactggg cgacaaagtt acccccgacg
    13981 accacgttac cgtcaaaggc agcatcatca aactcaaatg ggccgaccgc ctgccgcgca
    14041 tcatcctgta ttacaaacaa gaaggcgaaa tcgtttcccg cgacgacccg caaggccgcg
    14101 tcagcatatt cgaccgcctg ccgcaggccg ccagcagccg ctgggtcgcc atcggacgct
    14161 tggacatcaa caccagcgga cttctgattc tcactacctc cggcgaactc gtccaacgtt
    14221 tcgcccaccc cagcttcgaa gtcgaacgcg aatacgccgt gcgcgtactg ggcgggctga
    14281 ctaccgaaca aatgcgcagt ctcaccgaag aaggcgtgat gctcgaagac ggcttggcaa
    14341 aagtcgaacg catctacgaa caaggcggcg aaggcgcaaa caaatggtac aacatcgtga
    14401 ttaaagaagg ccgcaaccgc gaagtgcgcc gcatattcga aagccaaggc ctcacagtca
    14461 gccgcctcgt gcgcgtcgcc ttcggcccca tcggactgcc caaccgcctc aaacgcggac
    14521 agttctacga actcaacccc gccgaagtcg ccaacatcct caaatgggcg gatatgctgc
    14581 tgccgggcga acgccgccgc aaaaaagcct gaaagtaaaa atgccgtctg aaacacctgc
    14641 tgtttcagac ggcattttat tcgggcgttt tcaggagaaa aggtcgagtg ctttgacaaa
    14701 gaccatcacc acgccgtagg cgagcggtat gacggccaat gcccaacgcc accagaccga
    14761 tatgccgtcg ccggaaactt tttcgcccac aaggtaagcg tcggatatgg cggtttcgtc
    14821 gtcggggttg ccgctgcgtg cggcggtttt gatgtctttt tcgtggtgtt tttcgtgtac
    14881 ggatttgacg gcgaggttgc acaacaaacc gataatcagc aggcccgcca taatgtacat
    14941 ggttacgctg tatgcctgtg ccgccggtat gccgctgtcg atttggcttt ggcgtatgta
    15001 attgaccagt accgggccga tgacggcggc gattgaccag gccagcagga tgcgcccgtg
    15061 aatcgcgccg acctgatagg tgccgaacag gtctttcagg taggcgggaa tggcggcaaa
    15121 tccgccgccg tacatggaaa taatcacgca aaagccgatg atgaacaggg ctttgctgcc
    15181 gccttcgccg atggagggaa cggcgaaata cagcagcgaa ccgagtacga agaagatggt
    15241 gtaggtgttt ttgcgtccga ttttgtcgga aacgctcgac cacaaaaagc gtccgcccat
    15301 attaaacagg ctcaggaggc tgacgaagcc tgccgccgca cctgcgccga ctgccgcctg
    15361 cctgcctgcg gaggtttcgg aaaagagttc ctgaatcatc acggatgcct ggcccaatac
    15421 gccgatgccg gcggttacgt tcaggcacaa tacccagaac aacagccaaa actgcggcgt
    15481 tttcatggct tgggacacgt tgacatgatt gctgctgacc ggcttgtttt gcgttttcgg
    15541 cacggtatag ccttcaggtt tccagccgtc ggcaggtacg cggatggtaa acgcgccgaa
    15601 catcatcagt gcgaggtaaa acaggcccaa tacggcaaag gtttcggcaa ccccgaccga
    15661 agcggcgttt gaaaaggcgt tcatcagtga tacggaaagc ggcgaggcca gcattgcgcc
    15721 gccgccgaaa cccataatcg ccaaaccggt cgccataccc ggcttgtcgg gaaaccattt
    15781 catcagtgtg gaaaccggcc cgatgtagcc caaacccaag cctacgccgc cgatgacgcc
    15841 gttgcccaaa tagagcagga agaggttgtg cgtacgcacg ccgaatgcgg atacgaagaa
    15901 gcccaggctg aaacagcagg cggcggcaaa tatggctttg cgcggcccta cccgttccat
    15961 ccatgtgccg aacagggcgg ccgacgcgcc cagcatcgcg agtgcgatac tgaaaatcca
    16021 acctacggtc gtcagcttcc aatctccggc cgccgattcg gttatgccga taagtttggt
    16081 cagcggcgcg ttgaatacgg aataggcgta aatctgcccg atggcaaggt gcaccgctaa
    16141 tgctgcgggc ggtacgagcc aacggttgaa acccggcttg gcaatgcttg cctcacggtc
    16201 taaaaacttc ataacatcct ctttctgtca gttgaaaaat aaaatttcat ttgcccaatg
    16261 gaaacttatt aaaaattata aaaaaatatc gggttgggtt tttatccgcc ccaagatgcg
    16321 ccgtctgaaa catttcgggt gtgcggagag gtttctgttt tttccgacaa attcctgcgg
    16381 ctttccgctt ccggattccc gctttttagg gactggattc ccgttttcgc gggaatgacg
    16441 gcggaggggg agcggttttt ccgattgggt ttaaatgcaa tcgaacaaat cctgctgccc
    16501 ttgttctttg cttacgcgca cgtcggtttc gccgtcggag aaggtaatgt gcagcttctg
    16561 cccctgcttc aaaacatcgg cgttacggat gacttgtccg cgtgtatttt tgacaacgga
    16621 gaagccgcgt tccagaatgt gctgcggcga aacggcttcg agcaatgcgg cttgggcggt
    16681 caggctttga cggcgacggg cgagcagccg gctgaaggcg gcgggcaatg ccgtctgaag
    16741 gcggacgata tcttgccggt aaacggaaac atcaggacgg caatgttgca gggcttgggt
    16801 ttggcgttca aaacgggcgg tgtgggcgcg gaggttttgc gtcatcgagt aagacagcgt
    16861 ttgcgccagc ttgccgattg aagcgcgctg ctcgtcaagt ttttggcgcg ggtggcggat
    16921 ttgccgcgcg agccagtcga gtttttggct ggcatcgaaa tagcgttgct ccaaaacggt
    16981 tttcagacgg ccttgggctt ggacgaggcg gtgcagtgat tcttggcggt tggggctgac
    17041 cagttccgcc gcgccggtcg gcgtgggcgc gcgcacgtcg gcaacgaaat cggcgagcgt
    17101 gaaatcggtt tcgtgtccca cgccgctgac gacgggaatc gtgcaggctt cgatggcgcg
    17161 cacgaccggt tcttcgttaa acgcccgcaa gtcttcgatg ctgccgccgc cgcgacagac
    17221 aatcagcaca tcgcattcgg cgcgttgcga tgcggtttta atcgcttggg caatctgaaa
    17281 cccgctgccc gcgccctgaa cggcggccgg ataaacgatg acggggattt cgggcgcgcg
    17341 gcgtttcaag gtggttacga catcgcgcaa agccgccgcc gccagactgg taacgatgcc
    17401 gatacattgc ggacggacgg gcaaaggttt tttgcgttcc gccgcaaacg cgccttccgc
    17461 ctgcaactgc gccttcaacc gctcgtaggc ttcgtaaagc tgccccaaac ctttgagccg
    17521 tacttcgttt acggtaatct gaaattcgcc ccgcgcctcg taaatactga ttttgcccgc
    17581 cacttcgata tggtcgcctt ctttcaaagg ttgcgccaaa cgcgccgccg cacccttgaa
    17641 catcgcgcaa cgcacctgcg cgcggctgtc tttgagtgag aaataataat gcccgctggc
    17701 ggcgcgggtc agattggaca cttcgcccgc aatccacagg ccggcaaggt ggttttccaa
    17761 aatacttttg gcaaatgcgt tcaactcgga aacggacaac acgtcagaat gaaaaaaatc
    17821 agacatcgaa tcaatcaaat aataaaaaat atgaatatgt tttgaagccc aaggcggcat
    17881 cgtgccgtct aaattgtcaa caatattata acacgcgcca tcttgccgcc agccttttcc
    17941 tatatgactt ttttaagcgg ggaatgggaa aaatattcat caacccgccc tcaatctatt
    18001 caaattgcac cgccggcagg ctatgatgcg gatattttcg acaggaggaa aaatggatac
    18061 gcaggcagtt atcacacata ttgtccgatg gttggacgaa tacgccgctc aggcaaatgc
    18121 aaaagggttc gtcgtaggcg tttccggcgg catcgattcc gccgtcgtct ccacgctcgc
    18181 cgcccgcacc ggccgcacca cgctgcttct ggatatgccg atacgccaac accccggcca
    18241 gctcgagcgg gcaaggcggc acatccgcaa tctgcaaggg caatatgcca atgtaagcgc
    18301 gcaaacggcc aatctgaccg acaccttcca aacctttgag caaaccgtcg gcgttcatcg
    18361 gacggcattt gccaatcagc cgctttcgct cgccaacgcc agaagccgcc tgcgtatgct
    18421 gaccctgtac tactacgggc agatacacgg actgctggtt acggggacag gtaataaggt
    18481 tgaagatttc ggcgtgggtt tttttaccaa atacggcgac ggcggcgtgg acatcagccc
    18541 gattgccgac ctgaccaaaa cgcaggttta ccggcttgcc gaagcattgg gcgtggacga
    18601 ggcgattcaa aaagccccgc cgaccgacgg cctgtgggat acggaacgca ccgacgagga
    18661 acagatgggc gcaagctatc ccgaactgga gtgggcaatg ggcgtgtacg gcacgcgcaa
    18721 acccgaagat tttgaaggac ggcggcgcga agttctggaa atctatacgc ggcttcaccg
    18781 cgcgatgcag cacaaaatca acccgattcc tgtgtgccgc attccgcccg aattgctggg
    18841 ctgaaacatg aaaatgccgt ctgaaacgga aaaaccgtat ttcagacggc attgaaatat
    18901 ccgactccta tcccttaaga atcgagtacg cgggcaaaca gaatatcgtt ttccaaatga
    18961 atgtggtcgt tcaaatcgtc cgccatttct tttgccagcg cgtaaaggcg cgtccaactt
    19021 ccgcaagccc cttcgggtgc gtggaaatta ccggtcagct ctttgagccg tgcgatggcg
    19081 cggtcgtgtt cttcatgttc gtgcatcatc acgctgatgg gcatcgccgc accgcgtccg
    19141 acaccctgat taatcatcgg aaacagcatc cgctcttcct tcatcatatg catcagcagc
    19201 tcgtcccgca tatcggcaag cagcccggca atttccgccg gaaaggtatc ggcatggact
    19261 tgggccactt tctgcgccag cggcaccaat tcttcaaatt gtgcgcggtg gacattgtgg
    19321 tagcgttgca ggatatgatc aacggttgcg ccaaagggag cagcttccca aacggaaaaa
    19381 tcagtcattg cagtgttcct tttatagggt ttcgggtttg gttttgaaca ttcatacttt
    19441 aagaatctat ttaaacggag catacaccgc ccgcgcgttt ctgtacagcc tcaaaaacat
    19501 tccttatact ttgataataa aagtaatttt caaaaataaa atactgtccg aaccgttttt
    19561 tagaatttgc aaaggcgatt ggggcggtac agaaaaacta ttatcccgcc cgcccacttg
    19621 aaatttttat gcccaagccc tatcctgcac gctatcgtgc gaatcccaac cgaaaaggaa
    19681 aaataatgag cagcgaatta atcgtacaca ccggcgatgc cgccttcgag caagatgttt
    19741 tgaaatccga cctccccgtc ctgctggact tttgggctcc gtggtgcggc ccctgcaaaa
    19801 tgattgcccc gattttggac gacattgccg ccgaatttga aggccgtctg aaagtagtca
    19861 aaatcaacat cgacgacaac gaagccaccc cgtcccgttt cggcgtgcgc ggcattccga
    19921 ccctgatggt gttcaaaaac ggcgaagtcg tcgccaccaa agtcggcgca ttggcaaaag
    19981 gtcagctgac cgcctttgtc gaagcctcca tcgcctgata aagcgcaatc gaaaagccgc
    20041 cggaagattc cggcggcttt ttcgcaccct taagatttgt ggcggatttc ccaacaccgg
    20101 tggatttttt tgttgcggaa atcttcggga acggattgtt tggaaatgtc tttcacggcg
    20161 tattgttccg ataccgaatc gtccaaaacg aagctgcgca ggttgttgga aaaatacaaa
    20221 atgccgtctg aagcgagcag gttcaccgcg ccgtctatga gtttttgatg gtcgcgctgg
    20281 atgtcgagga tgccgggcat tttcttgctg ttggaaaaac tgggcgggtc catcacaatg
    20341 aggtcgaact gcctgccttc cccatatgcc gtctgaaggt attggaacac gtcggcgcgg
    20401 acgattttgt gccgttccat atcgatgccg ttcaattcaa aattgcgcct cgcccaatcg
    20461 agataagtgt tggacaaatc gacggtttcg ctggatgccg cgccgccggt ggcggcatag
    20521 acggtgaagc tgccggtgta ggaaaacagg ttcaaaaaac gtttgcccgc cgccgtttcg
    20581 ccgacttttt tgcgcgtgtt gcggtgatcg aggaagaagc cggtgtcgag gtatttgtcg
    20641 aggttgaccc aaaacttgcg gccgttttcg gcaatgacga aatcgtcgcc cgccttgccg
    20701 gttttctcgt actgctgcaa acctttttgg cgttcgcggc gtttgagacg gatttgttcg
    20761 ggttcaaaac cggtaacgaa agcgacggct tccaatactt cggcaagcca cgcttcgtat
    20821 tctccgggcc gcatcagcca gccggtatcg tattcctgaa ggtggattcg atcgccgtaa
    20881 acatcggcgg caaaagggaa ttgggggatg tcgcggtcgt aaatgcgcca ggcttcgatg
    20941 ccgttgcgtt tcgcccattt cataaggtgt ttgatgtttt tgcccaagcg gttggcaaac
    21001 ggtgtgatgt cggtcattga tttcaggcgg aataaagtgg aaaacggaaa ttttactgta
    21061 attaacgccc gattgcttga ccttttcggg caaaccctat accatccgtc gcttatcttg
    21121 tcatacgaag ccatcgcctt ccaacctaaa ccgcccttac gggcgcgttt cttctgttgc
    21181 tttgattttg caaagcatat ctgtgcaggt tgccgtcgat gtaaaccaca agcaagccgc
    21241 ttgcgacaac cctgtaactt cacattcccc gtatcgttac ccttccctgc ttcaggccgt
    21301 ctgaaccttt cggacgcggg cgttgttgtc ttccaaggat agccatgtct attaaatttg
    21361 ccgatttgaa ccttgataaa aacattttgt ccgccgtcag cagcgagggt tacgaaagcc
    21421 cgacgccgat tcaggcgcag gcgattccgt ttgctttgga aggctgcgac atcatggctt
    21481 cggcgcaaac cggctccgga aaaaccgcag cctttctgtt accgactttg cagcgactga
    21541 ccaaacgcag cgaaaaaccg ggcaaaggcc cgcgcgcgct ggtgttggca ccgacccgcg
    21601 aactggcggc gcaagtcgag aaaaacgcgc tggcgtatgc caaaaatatg cgctggttcc
    21661 gcaccgtcag catcgtcggc ggcacatcgt tcggctacca aacccgcgcc ctgagcaaac
    21721 cggtcgattt gattgtcgcc actccgggcc gtctgacgga cctgatgcaa agcggcaaag
    21781 ttgattttga acgtttggaa gtgctgattt tggacgaagc cgaccgtatg ttggatatgg
    21841 gctttatcga cgacatcgaa accatcgtgg aagccacccc ggccgaccgt cagactttat
    21901 tgttctccgc cacttgggac ggcgcagtcg gcaaactggc gcgcaaactg accaaagact
    21961 ctgaaattat cgaagtcgaa cgcgtggacg gccaaggcaa aatcgaagag cagttgttgt
    22021 actgcgacga tatgcgccac aaaaaccgcc tgctcgacca catcctgcgc gatgccaaca
    22081 tcgaccaatg tgtgattttc acgtccacca aagcgatgac cgaagtcatc gcggatgaat
    22141 tgtacgaaaa aggtttcgcc gccaactgcc tgcacggcga tatgccgcag ggctggcgca
    22201 accgcacgct gacggatttg cgtaaaggcc gctgcaaaat tttggttgcc accgacgttg
    22261 ccgcacgcgg tattgacgta ccgaccatta cccacgtcat aaactacgac ctgccgaaac
    22321 aggcggaaga ctacgtccac cgcatcgggc gcaccggccg cgcaggccgc acgggtattg
    22381 cgattacgtt tgccgaagtg aacgaatacg tcaaagtcca caaaatcgaa aaatacatta
    22441 accgcaaact gcccgaactg accatcgaag gcatggagcc ggcccgcaaa cgcaaatccg
    22501 caggcggcaa gccgaaaggc aaaggcggct ggggcgatag gaaatccggc ggccggcgcg
    22561 gcgatcataa accgggcaaa gaaggcttcg gcggcaaaac gcgcggcgaa ggtttcaaga
    22621 aagaaggctt taagaaagac agtttcaaaa aaaccggcga aggcttcaaa ggcaaacgca
    22681 aagccggcga ctcttttgca ggcaaaagcg aacgccgtta caaagaccgc taagccccaa
    22741 cctgccgcgt aaaccaatgc cgtctgaaac cgatttcgag tttcagacgg catttttgta
    22801 atgtttcagc accgcccggc tttgataccc aaaggattaa tctgcaatat agtggattaa
    22861 atttaaaccg gtaccgcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg
    22921 tcgccttgtc ctgatttttg ttaatccact ataaaaaccc ttttccgctt tggcaacgat
    22981 tgaaaatttc cgtaaattca aatatctaga ttccttcctg catgggaatg acgcagaagg
    23041 gtttcagatg caggatggac attcctgcct acccaatctc gccctacggc ttgacggttc
    23101 gatatgatgc cgtctgaaaa cccaacggcg gtatgacaat gccgccctgc caacgcacgt
    23161 agatcagaat tgccatcccg acatcaaacg cttggaaaca aaatgccgtc tgaaaatcaa
    23221 acggcaacat aacaatgccc ctaacaaata caaaaatgcc gtctgaaagc ccttcagacg
    23281 gcattgacgc gccgggttta ccgccttctg ccgaaaccgc gcatagcggg acggcggtaa
    23341 ttgacgggcg ggcgcgttgt cgggcggtaa cgctgcgcct gcgccgcctg ttgttttgca
    23401 cggaggctgc gcgtgttcaa atccctgctg gtgcgcgcat tggggcgtgc ggactgatgg
    23461 taggcagcac gcgcgcgacg ggcgacgggg ctgtcttggt tgcccgcccg tgtgaatttg
    23521 tttgccagcg cgttgccgat aaacgcgcct gccgccgcgc caatcaggct ttgcagcagc
    23581 cagcttcctg tcgattggtc gtaaatatac tgctgcccgt ctttgccggt aacgggctga
    23641 ccgttgttgc cgtttgcctg cgcctgggca ggaatggtgt ctttgactgc ttcgggagtc
    23701 agttggtaaa ccgtatcgtc tgcctgctgt gcgagctgct gttgcagggc ttcaatctgt
    23761 ttctgctgcc gttcgagccg cgcctgcgtg tcgtcttggc aggcggtgag tgcgaatgtt
    23821 gcgataagcg cggaggcgat gatttttttc atgtgtgtcc tgttcgggtg gaaaatcggt
    23881 tgtattgtat cgccgtcggg aattttggca agcattctgc cggcaaatcg tgatgtttac
    23941 aggggtaggg tgtgcagttt gcggacaaat gcgaggctgt tggcgacagg gttgcctttg
    24001 ttttcgactt cgtgttcggt ttctatggtc agcaggcggc ggtttttgtg tttgttcagg
    24061 ctcaattcgg cttgtgcggg tgtgaaaaac aggcggtgtt tttcggcaaa gcggcaggcg
    24121 cgcttgtttg cggttttcaa aatctgaagc agcgatacgt ccagatggaa gcgtcgcacg
    24181 cccaatacga gaatccgtgc ggcaaaaaaa tcggcggcat cggtaaactc ggcggggctg
    24241 ctgcggctga agtcgtgccg ttcggcggct atcagggcgg caacggtgcg gatttgcgga
    24301 atcatcgatt cgctgatgag cgtgtcgtcc cgccctgcat cgagaagcat gggggaaaaa
    24361 tcctgcgcat catcgacaat aatgctgcaa gtgtgcaggt tttcgttttg tacggcggtt
    24421 tggggcattg catccatggt cattttcctg attctgtcgt gtgttgccga atcgggcgac
    24481 ctgtgtgaag gtaacaaaaa agccgccccg ttttcgagcg gcctgttttg cgtatgggat
    24541 ggatttcaag caagcgcaaa aaagcaccgc acgtctgtgt gataccaata gcagtaagcg
    24601 gttgtaaatg ttttgccttg catgatgaaa tgccgtccga agataaaaat attggggaga
    24661 ttctaaatca aaacgctgcc gcgcctcaag cattatatcg aacttttttg ttttttcagc
    24721 tatccggttg aaaatattta ggtttatttt taccactgcc cgatattgtc ggcaatttcc
    24781 ctttatccgc cttgaaaaac ggtgcataat cccgagcaaa accgcaatca ggagcaatta
    24841 tgcaaaacta tctgaccccc aatttcgcct ttgccccgat gattcccgaa cgcgcttcag
    24901 gcagccgcgt ttgggatacg gaagggcgtg aatatattga tttttcaggc ggcatcgccg
    24961 ttaacgctct ggggcactgc caccctgccc ttgtcgatgc tttaaacgcg cagatgcaca
    25021 agctgtggca catttccaat atctatacga cgcggccggc gcaggaattg gcgcaaaaat
    25081 tggtgaaaca cagttttgcc gacaaggttt ttttctgcaa ctcgggcgcg gaagcgaatg
    25141 aggcggcgtt aaagctggca aggaaatatg cgcgcgaccg tttcggcgga ggcaaaagcg
    25201 aaatcgtcgc ctgtatcaac agtttccacg gacgcacgct gtttaccgtg tccgtcggcg
    25261 gacagccgaa atacagtaag gattatgcac cgctgccgca aggcattacg cacgttccgt
    25321 tcaacgatat tgccgcgctg gaagctgccg tcggcgaaca gacctgcgcg gtcatcatcg
    25381 agccgataca gggcgaaagc ggcatcctgc ccgccactgc ggaatatttg caggcggcgc
    25441 gccgtctgtg cgaccggcac aatgcgttgt tgattttgga cgaagttcaa accgggatgg
    25501 ggcatacggg caggctgttt gcctatgaac attacggcgt tgttcccgat attttgagtt
    25561 cggcaaaagc cttgggctgc ggctttccga tcggcgcgat gctggcgaca gaaaagattg
    25621 ccgccgcctt ccaaccgggc acgcacggct cgactttcgg cggcaacccg atggcgtgtg
    25681 cggtcggcag ccgcgccttc gacatcatca acgcgccgga aaccttgcac aacgtccgca
    25741 gtcaggggca gaaacttcag acggcattgc tggatttggg caggaaaaca ggcttgttct
    25801 cacaggttcg cgggatgggg ctgctgctcg gctgcgcgtt ggacacgcct tatcgcggac
    25861 gctcatccga aatcgccgct acctccttga aacacggcgt gatgatctta gttgcgggtg
    25921 cggacgtatt gcgtttcgcg ccttcgctgc tgttgaacga tgaggatatt gcggaaggtt
    25981 tgcggcgttt ggaacacgtg ctgacggaat ttgccgccgc aaaccgcccc taggcagaaa
    26041 gcagatgccg tctgaagacg ggaaggcctc agacggcatc agaaacaaaa aaccgcttcg
    26101 gaaacgtgat ccaacgttcc gaagcggttt tgtttgccat caggactcga acaccaattc
    26161 cggttccctg ccctcttcga tgactgcctt gccgaccacc acttttttca agccttgcaa
    26221 atcgggcagg cggtacatcg tatcgagcag gcagcgttcg acgatggaac gcaggccgcg
    26281 cgcgccggtt ttgcgttcca ttgcctgccg cgcgatggaa cgcaatgcgc cttcttcaaa
    26341 ttccaacccg acgttttcta tgccgaacaa ggcttgatac tgcttgacca aagcgttttt
    26401 cggctcggtt aaaatattaa tcagcgcgtc ctcatccagt tcttctaaag ttgcaatcac
    26461 gggcaaacgt ccgattaatt ccggaatcag gccgaatttg attaaatctt ccggttcgac
    26521 gatgccgaac agcttggtaa tgccggcatt ttcgtccttg ctgtgaacgg acgcgccgaa
    26581 accgatacca cctttctcgg tgcgctggcg aatcactttt tccaagcctg caaacgcgcc
    26641 gccgcagata aacaggatgt tggcggtatc aacgttgata aattcctgat tcggatgctt
    26701 gcgtccgcct tggggcggaa cgcttgccac cgtaccttca atcagtttca gcaaggcttg
    26761 ctgcacgcct tcgccggaca cgtcgcgcgt gatggacggg ttgtcgcttt tacgcgaaat
    26821 tttgtcaatt tcgtcaatat agacaatgcc gtgctgggct ttttcgacat cgaaatcgca
    26881 tttgcccaac agtttggtaa tgatttgttc gacatcttcg ccgacatagc cggcttcggt
    26941 cagcgtggtc gcatccgcca tcacgaacgg cacgtccagt ttgcgtgcca aagattgcgc
    27001 caacagcgtt ttacccgatc cggtcgggcc gataagcagg atgttggatt tcgacaattc
    27061 gacaccgccg tcggctttcg ggtggcgcag gcgtttgtaa tggttgtaaa ccgcaaccgc
    27121 cagcgccttt ttcgcctgcc cctgcccgat gacataatcg tcgaggttgg caacgatttc
    27181 ggcgggcgtg ggcagtttgc cggattcttc cggctcccct ccggcatttt ccggaggcgt
    27241 gccgccgttg tcgccttcgt acaatatttc gaggcagttt gagacgcatt cgtcgcagat
    27301 aaaggcgttt tcgccctcaa tcaaatgttt gacgtgtgat ttggattttc cgcaaaagga
    27361 acaggtacgg ttttcgttgg acatggcttt ctttacaatg tgtgcgttgc agaaacggca
    27421 tgcgccgttc gggttgccaa gtataataac tatatccgtg cttatcaacg tattacctta
    27481 aaatcccgcc gattcggcta taatacgccc ctttgcaacc gccccggcgg cagaaatgcc
    27541 gtctgaaacc aaatctgagg atattcatga gaaaacccca acgcggctac gcccgccaag
    27601 accgtgtcaa agaacaaatt atgcgcgagc ttgccgaact cgtccgtacc ggactgaaag
    27661 acccgcgcgc cggcttcatc actatcaacg aagtcgaagt tacccgcgat tacagccacg
    27721 ccaccgtgtt ctacaccgtt ttgaaccaag atacgcgcga aattacggaa gaagtgctgg
    27781 aacacgcgcg cggacacctc cgcagcgaat tgtccaaacg catcaagctg ttcaaaatcc
    27841 ccgaactgca tttcaaatac gacgaatctt tggaacgcgg tatgagcctg tccgccctta
    27901 tcgatcaagt ggcggcggaa aaaccggttg aagactgata ctgccagaat gaacaatctg
    27961 attcccgaac atttagccgc ctatgcacat agtgataacc tgcaaattga aggcgggcat
    28021 cgttgctttt cattatcctg ccaaggtaga gatactttcc acatccgtta ctatggagag
    28081 ccttttgatg gattgattac cgatactgat aaggcgccgg taaaaattgt ggcggtagaa
    28141 gctgtaagcg gcgatgaaat cgtattgttt gatggggcgg aacatggcta taacgctatg
    28201 ttctgcgaca aatatagcca aaatcaaaag caaaacagaa cgttaactga tttggatgaa
    28261 tatacttacc gagttccgat tcatctttat tacaatatag actacgaaga tgaatatgaa
    28321 gatttcgtca attctgaagg acaagtcccc ttaattgatg gccgcatcat tagttttgac
    28381 tcattaaaac gaaatggctt tgatgcaatc agcattgatc taattgatga aaaacactct
    28441 gttcgtgaat tattgaatga agaattaagc tgacacgcag gccgtccgaa ccctttcaga
    28501 cggtctgaac attaatattc cctaattaat atgaccaata aacccgccaa acgcccggtc
    28561 aacggcgttc ttcttctcga caaacccgaa ggcctttcca gcaacaccgc cctgcaaaaa
    28621 gcgcggcgtt tgttccatgc cgaaaaagcc ggacataccg gcgtgctcga tcctttggca
    28681 accggacttt tgcccgtctg cttcggcgaa gcggccaagt tcgcccaata tctgattgat
    28741 gccgataaag cctacaccgc cacgctgaaa ctcggcgaag ccagcagcac gggcgatgcc
    28801 gaaggagaaa tcattgccgc cgcccgcgcc gatatttcct tagccgaatt tcagacggcc
    28861 tgccaagcac tgacaggcaa catccgccaa gtgccgccga tgttttccgc cctcaagcac
    28921 gaaggcaaac cgttgtacga atacgcgcgc aaaggcattg ttatcgaacg caaaccgcgc
    28981 gacattaccg tttactctat cgatattgcc gagtttgacg cgcccaaagc cgtgataagc
    29041 gtgcgttgca gcaaaggcac ctacatccgc accctcagcg aaggcatcgc caaacacatc
    29101 ggcacattcg cccacctgac cgccctacgc cgcaccgaaa ccgccggctt taccatcgcc
    29161 caaagccaca cgctcgaagc tttggcaaat ttaaacgaaa cagaacgcga cggtctgctg
    29221 ctgccctgcg acgtattggt ttcacacttt ccccaaaccg ttttaaacga ttatgccgtc
    29281 catatgctcc aatgcgggca acggccgcgt ttcgaggaag acctgccttc cgatacgccg
    29341 gtacgcgttt acacggaaaa cggccgcttt gtcggtttgg cggaatatca aaaagaaata
    29401 tgccgtatga aagccttgcg cctgatgaac acggcggcat cctccgcctg agcggcggtt
    29461 aaaaatacgg gctgcgtttg aataatgtgt tgatatttcc gtaaaatcac gacacactcg
    29521 gacacccgcc ccgcttatcg caactttgcg aacttccccc ggaaacagca aagacatcaa
    29581 agaattgatt ttattataat ttatttgtaa agccatttgc cgttacacaa gaatggcaca
    29641 ttaaaataac tgatgaggat ttataacgat gaagacaggc attcaaaccg aattagccca
    29701 agccctgcta tcacacgaaa aaatatgggc gaacgaagaa aaaaccattt tggccaaaaa
    29761 catcctgttg gatttggtgg aaaaaaccga cccgtccatt atcggtttgt tattgaataa
    29821 tgatgagtta aaacgccatt tctttgtgga agtgaatggt gttttggtgt ttaaattgca
    29881 ggatttccgt tttttcttgg acaaacacag catcaataat tcctacacaa aatacgccaa
    29941 ccgcattggt ttgacggacg gcaaccgctt tttgaaagac aattcggata ttgtgttgga
    30001 ttttccgttt aaagattgcg tattgaatgg cggacaaagc accgaagaag gcgaagaaat
    30061 ttattttaaa cgcaataata gccagccagc cagccagcca gccagccagc cagccagcca
    30121 gccagccagc cagccagcca gccaattata caccaaatta accagaaaaa gacaagaaat
    30181 cttttttaat caaacgcttg cttttgatga aattgaccgg ctttttgacg caaaagcatt
    30241 ctcaaaattc tcccgccaca ccgcagacgg caaacaaccc gttggcgaaa tcaaacgccg
    30301 ttcagacggt acgccagccg aaaatcttat tatcaaaggc aataatctga ttgccctgca
    30361 ttcgcttgcc aagcagttta aaggcaaagt aaagttgatt tatattgatc caccgtataa
    30421 tacagaaacc gatagctttg catataacga taaattttca cattccactt ggctaacatt
    30481 tatgaaaaat cgcttggaaa ttgcgaaaga gttattaaaa gatgatgggc taatctttgt
    30541 tcaatgcgat gataaggaac aggcttattt aaaagtttta ttagatgaaa cttttacaag
    30601 agaaaatttt ataaactgca ttgctgtaaa aatgtcagag ccatcaggta acaaaatggc
    30661 acatacatcg catagactac caaagataaa ggaatatatt cttatctata agaataaaaa
    30721 tataaaatta aaccctatta gagagcaaaa atcagaatgg gacaatgagt acaatatttt
    30781 tttagaaaat tttacacaag aagataaaaa atttattgat ttaatagtaa atagccaaac
    30841 agaaaataaa gaaataaatg gtaatacttt aaaagaaatt gatattcttt taaagaaaat
    30901 atctcctatt tcagttaatc aaaaattagc ccaattaaat attaaagata atgaagttat
    30961 aaaatggaaa ctagataatg cgtatagaat tgtaagaaca gcagctagta gtagcgtaaa
    31021 aaaattagca gatgaaaaaa aagaaatttg ccaacaacaa tttttttcag ttatttctaa
    31081 acgagataaa ttattgtata tcgtcaagtc agattactca aaagatgcaa aagcaccacg
    31141 agtgcaggtt ttgtttgcag aagattattt gagtattagt ttatgcgatt tatggacaaa
    31201 tataaatacc acaggactag aagcagaagg aaatgtagag ttaaaaaatg gtaaaaagcc
    31261 agaaagttta attgaaacca tcattaaact agcaaccaac gaaaacgaca tcgttctaga
    31321 ctaccacttg ggcagtggca caaccgccgc cgtcgcccat aaaatgaacc gccaatacat
    31381 cggcattgaa caaatggatt atattgaaac gcttgccgtt gaacgcatga aaaaagtaat
    31441 tgacggcgag caaggcggta tctcaaaagc tgtgaattgg caaggtggcg gcgagtttgt
    31501 ttatgccgaa ctttccccat ttaacgaaac cgcaaaacaa caaattttgg cgtgcgaaaa
    31561 ttcagacggc atcaaaacac tgtttgaagg tttatgcgaa cgccatttct tgaaatacaa
    31621 cgtcagcgta aatgaattta gccaaattat tgaagagcct gaatttcaat ccttggcatt
    31681 agacgaacaa aaacaaatga tgcttgaaat gttggattta aatcaaatgt atatttcatt
    31741 atccgaaatg gacgatgaac aatttgcagg ctgcctgaat gatgatgata aagcgttaag
    31801 ccgtgcgttc tatcaatcag taaaacatca agcggagaaa aaagatggcg aataataaaa
    31861 cgttgtttga ttgggtggaa gaccgcaaat caatgctgga agaaatggaa cagacggatt
    31921 ttttcgcgct gcctgaattt gtttccaata atttaaaata tccgtttttt gaatggcaaa
    31981 aatcggcttt agaaaatttt gtgatttttg accgcacttc aaaattaaag gatttccccg
    32041 acatcaaaaa ccgccctacc catttgctgt tcaatatggc aacaggtgca ggcaaaacga
    32101 tgatgatagc ggcattgatt ctgtattatt ttgagaaagg gtatcggcat tttttatttt
    32161 tcgtcaataa aaacaatata gtatataaaa cggaaaataa ttttatcgac ccgacgcatc
    32221 caaaattttt atttaccgag aaaattttgc aaggtgatac ggtaattccc attcgcaaag
    32281 tggagacatt tagccaatat tcagacggca ttgaaattaa atttaccagc attcaaaaac
    32341 tatataacga tattcacacc gagcgggaaa atcaaaccac attggcggat ttgcacgaat
    32401 taaatcttgt gatgctgggc gatgaagcac accatttaaa cgcgcaaacc aaaggcaaaa
    32461 aacaaggcga attagattta gaaaaggaaa tgaacgagcg taccagcgat gccgaatttg
    32521 aacgcaaagg ctgggagcat atggttttgg agttgttact caataaaaat ggcaatcccg
    32581 gccaaaatgt gctgttggaa tttaccgcca cgctgcctga aaatactgaa gtgcaacaaa
    32641 aatatgctga taaaatcatc acaaaatttg gtttaaaaga atttttgcaa aaaggttata
    32701 ccaaagaaat caatttggta tccggtacgc tgaataagaa agagcgggtg ttgcacgctt
    32761 tattgtttgc ctggtaccgc catcaaatcg cgttgaaata cggcattgcc aatttcaagc
    32821 ctgtgatgtt gtttagaagt aagacgattg atgaatcaaa agcggattat tcggcgtttt
    32881 taaattgggt agaaaatgtg caagcggatg atttttcgtt tttaactaca ttttcaacaa
    32941 gcttaaacga tagcaataac gccaacgaac aaggcaaaac ccgtaccgaa caagccctga
    33001 aatttatgca ggaaaataaa tttgaatttg tgcatttggc aaattggatt aaacaaaatt
    33061 atcaaaaaca caatgtcatt attaccaact ccgaaaccaa caaaaacaaa actgaaaaaa
    33121 ccgacagcga aaccgaaaaa ttgctgaaca atctggaagc ggctgataat ccgatccgtg
    33181 ccatttttac ggtggataga ttaaccgaag gttgggacgt tttaaatttg tttgatattg
    33241 tgcgtttgta tgaagggcaa aacggtggcg gttcgaataa aaaatcaggc aaaacggctg
    33301 ccgccaccgt atcggaaaag cagttgattg gtcgcggcgt gcgttatttt ccatttgcgt
    33361 ttgaaggtaa acagccgaat aaacgcaaat ttgacaacga tatgcaacat gaattgcgta
    33421 tcttggaaga attgttttat tacacgcacg atgagcaatc ccgctatatt acagaactga
    33481 aaaacgagtt acgaaaagac ggttatttgc ctgaaaaaga cgatgataag gtattggcaa
    33541 catttaagct caaatctgaa tttgcagata ataaggattt tagagagttg ttaatttggg
    33601 cgaataaaaa aatccccaac ccaaacgcca aaagcaataa tgcagacagc ctgcaagcca
    33661 atccgcaaac gctttcattc caagttcacg gtaatcaact gttgcaggaa acgcaattta
    33721 cagccgatga aaatgatgaa acagcccgac aaatcggcac acaaaataat tttactcaaa
    33781 ccataaaaat gagtgaaatg gagcggcata ttttcaataa agcattacat atcaaaggga
    33841 aaaatagtca atctttattc cattttgacc gcctgcaaag caaactcgac attcaaaatc
    33901 gcaatgaatt gcaaaataaa ttgttgaaag actggcagat tgaatttttg ggattagagc
    33961 aagataaaca ggttcgcccg gatgataaac ttgcaggctg cctgaaaatc ttggaaatgg
    34021 ttgaaaaaca tctgaatgaa agtgatatac cgtttatcgg tacaaaagaa tttacaccta
    34081 aaaaattgtg ggaaattttc ggcacgccaa aacaaaaatg ggtcaaaaaa gatgatataa
    34141 aaactgctat tgccacgcaa aatgattggt atgtgatgga taattttgcc ggaacgggtt
    34201 tggaagaagc gttaattcaa tttatttcag cgcgcttggg cgatttgaaa tctcaatatg
    34261 acgttcattt aatccgcaat gaagaagtgt ttaaattgaa taactttgcc gatggcgaag
    34321 gatttatgcc ggactttgtt ttattgctga aagataaaca aaaatcttct tccgacagcg
    34381 tggacgactt tttgcattac caaattttca ttgaaccgaa aggcgggcat ttagtggaaa
    34441 atgattcgtg gaaagaagct tttttaaaat caatcacagt ggaatatggg agggataaga
    34501 tcctgcaaaa aaatacaccg cattatcgtt tgatcggttt gccgtttttt actgatcatc
    34561 agaaaaatgg acaatttaca gagttattcc ctttaggtgc ggcatcgctt gaaaaataga
    34621 gtagtgcatt gcaggcagcc ccgttcggca aaacttcctt tacaaaaggg cgttttgtca
    34681 gatatttaat caacacatta ttaaaataca accaaatttt aatgccgtcc gaaccatgta
    34741 ttcagacggc atcgtatttt tcagtatcta aaccgtttcc ctgccccaat ccttactcct
    34801 caaaatcgag gcatcgacat cttgaatatc gcggtgtccc gtaaacgcca tcgacacatc
    34861 catttccttg tacaaaatct ccagcgcgcg cgttacgcct tcttcgccat acgcacccaa
    34921 accatacagg aacgcccgac ctatcatcgt gcctttcgca cccaaagccc acgccttcaa
    34981 aatatcctgg ccgctgcgga taccgctgtc catccaaact tcgatgtcac tgccgactgc
    35041 ggaaacaata tcgggcaagg ccttgatggc ggacacagta tcgtcgagct ggcggccgcc
    35101 gtggttggaa acgaccaatg catccgcacc gcttttcgcc gccttttccg cgtcttcggg
    35161 ttccataatg cctttgataa tcagcttgcc gccccacaaa tctttaatcc gcgcaacgtc
    35221 gtcccagctt aagcgcgggt cgaactgttc cgccgtccac gaagaaagtg aagacaaatc
    35281 gccgacgttt ttggcgtgtc cgacgatatt gcggaacgtg cggcgttccg tgttcagcat
    35341 tttcatgcac cattcgggct tggtcgccag attgattaaa ttggcgatgg tcggtttcgg
    35401 cggcgcggac aggccgtttt tgatgtcttt gtggcgttga cccaaaacct gcaaatcggc
    35461 agtcaatacc aatgccgaac atttggcatc tttcgcacgc ttaatcaggt tttccataaa
    35521 ttcgcggtcg cgcatcacat aaagctgaaa ccaaaacggc gcgctggtgt tctcggcaac
    35581 gtcttcaatc gagcagatgg acatggtgga caacgtaaac ggaataccga acttctccgc
    35641 cgcacgcgcc gccaaaattt cgccgtctgc gtgtgccatg ccggtaaaac ccgtcggcgc
    35701 aatcgccacc ggcattttca catccccccc gatcattttg gtttcgaggc tgcgtccctc
    35761 catattgacc aatacctttt gtcggaagcg gatatctttg aaatccgaag tattctccct
    35821 ataagtcgtt tccgtccacg aacccgaatc gatgtaatcg taaaacatac gcggcatttt
    35881 gcgcttggca acgcggcgca agtcttcgat gcaggtcatt ttgctcaaat cacgtttcat
    35941 cttgtttttc cctgtttaac agcctgatgg aatatgccgt ctgaaactgc ttgagccttc
    36001 aaacggcatt tcgggcaata cccaaataaa tcactaagat ttagataatc tatatcaata
    36061 ttaaaaataa atcaaatcgt tttgaaaccg cttgccggca aaacgcccca accgccgctc
    36121 ctcaaaaaac ataactaact gattcaaaaa accaaacaaa taaagcaaaa tcagactata
    36181 ccgccattta aacaaacaat cggcaacagc tcccccatac ttgactgaaa cactcagata
    36241 ttggacaatt ccgcccacta taaaaaagcc gacacgggca accaccacca tgagactgac
    36301 caccaaaggg cgtttcgccg ttaccgctat gctggatttg gcgatgaacg cgcaaaccgg
    36361 cgccgtcaaa ctcagtgcca tcagcgaacg ccaaaatata tccctctcct atctcgagca
    36421 attgttcggc aaactccgcc gcgccggact ggttgaaagc ctgcgcgggc ccggcggcgg
    36481 ctacatcctc gccgcccctc ccgcacaaat caacatcgcc caaatcattt ccgccgccga
    36541 agaccgtctg gacgcgaccc aatgcggcag caaagccaac tgccaccacg gcgcgccctg
    36601 cctgacgcac gacctttggg agaatttaaa caaaaccatc aacgactatc tcggcagcgt
    36661 taccctgcaa agcatcatcg aacagaaaaa caacggcgac ggcagccgcg tcgtccaatt
    36721 tacacacatc cattaaataa cacccgaaaa agaaagagca aaccatgacc gtcaaaaccc
    36781 ccgtttacct cgactacgcc gccaccacac ccgttgacaa acgcgttgcc gaaaaaatga
    36841 ttccctatct gactgaaacc ttcggcaacc ccgcctccaa cagccacgca ttcggctgga
    36901 cggcagaaga agccgtcgaa aaagcccgcg ccgacatcgc cgccctgatt aacgccgacc
    36961 ccaaagaaat cgtcttcacc agcggtgcga ccgagtccga caacctcgcc atcaaaggtg
    37021 cggcaaactt ctacaaaacc aaaggcaaac acctcatcac cgtcaaaacc gaacacaaag
    37081 ccgtgctcga cacgatgcgc gaactcgaac gccaaggctt tgaagttacc tacctcggcg
    37141 tgcaggaaaa cggtttgatt gatttggaag aactcaaagc cgccatccgc gacgacacca
    37201 tcctgatttc cataatgtgg gtgaacaacg aaatcggcgt ggtgcaaaac attcccgcca
    37261 tcggcgaaat ctgccgcgaa cgcaaaatcg ccttccacgt cgatgccgcc caagcctgcg
    37321 gcaaagtgcc tgtcgatgtc gaagccgcca aaatcgactt gctctcgatg tccgcgcaca
    37381 aagtgtacgg ccccaaaggc atcggcgcgc tgtacgtccg ccgcaaaccg cgcgtccgcc
    37441 tcgaagccca aatgcacggt ggcggtcacg agcgcggttt ccgttccggc acattgccga
    37501 cccatcaaat cgtcggcatg ggcgaggcct tccgcatcgc caaagaagaa ttggcacaag
    37561 acacagcgca ctacctgaaa ctgcgcgaca tcttcctcaa aggcatcgaa ggcatcgaag
    37621 aagtctatat caacggcgac ctcgaacacc gcgccccgaa caacctgaac gtcagcttca
    37681 acttcgtcga aggcgaaagc ctgattatgg cggtgaaaga actcgccgta tccagcggat
    37741 ccgcctgcac ctccgcctcg ctcgaaccca gctacgtcct gcgcgcgctt ggccgcaacg
    37801 acgaactggc gcactcatcc ctgcgcatca ccttcggccg catgaccacc gaagaagaag
    37861 tgcaattcgc ggcagaactg atcaaatcca aaatcggcaa actgcgcgaa ctgtcgccgc
    37921 tgtgggaaat gttcaaagac ggtattgatc tgaactcgat tgaatgggcg gcgcattaag
    37981 attgatgtca aacaaaggct gtctgagaaa gaataccatg aaagttaaaa ttattaattt
    38041 gccaaatggt tataaacgta ttatttatgg aaaatatttt gaacaatttg acttagatta
    38101 tgaacaagat ttagacgtat taaaaaaaga tattgaattt gctttatctg ttattgaata
    38161 caatcgatct atattcaaaa aattctcttc cttatttgag aataaaatca tttttgttta
    38221 tcaaggagga catcatcttg atattattga tcgtgataaa ggaagtttga aatagttaac
    38281 tattgaagat cattggacaa aagatgttga tgatatcact ggttcatgga aaatggagaa
    38341 tttagaaatt agccgacaaa ctcaatatgc aatgcaactt ctaaagattg gtaaaacaaa
    38401 tattgagaaa gaaataagaa aaattaaaca aaggttctaa aaaaatgagt aaatacggtg
    38461 gtgaagacgt aatctatatg aatgatggtg tatttagaaa taaattagcc attcaagatc
    38521 cacttaaatt taaaaaaggt cgaaagagac atttctgcta tttccgccca aaatctgctt
    38581 ttgcaaccca ttacaggcaa tttcgatttg gcgcatttac agaccattca tcgcgaactg
    38641 tttggcaatg tttatgattg ggcaggaaaa atccggaggg ttgatatttc taaaggtaat
    38701 acccgatttg ccaattttgc ttttatcgaa aacgaaagcc gtaaactttt ggagaagctg
    38761 aaaaacgaaa actatttgcg ggtgttggat aaagataaat tcgccgagcg tgcagcctat
    38821 tatttggacg agttgaacgt cctacatcct tttcgtgaag gaaacggcag aacattgcgc
    38881 ctgtttatga cacaattggc aataaaaaac ggttttcaga tacattggca aaatatctct
    38941 gccgaacaaa tgattcaagc ctgtattcag gcataccatg ccgatagcag cttattggca
    39001 cgcctaatta ttagacaatt tagaacaatc gtctttttca gacgactaaa acatcaacaa
    39061 accacgatat tttaaggaaa ccacatcatg gcatacagcg ataaagtaat cgaccactac
    39121 gaaaatcccc gcaacgtcgg cactttcgac aaaaacgacg agtccgtcgg caccggcatg
    39181 gtcggcgcgc ccgcctgcgg cgacgtgatg cgcctgcaaa tcaaagtgaa cgatgaaggc
    39241 atcatcgaag atgcgaaatt caaaacttac ggctgcggtt ccgccatcgc ttcgtccagc
    39301 ctgattaccg agtgggtcaa aggcaaaagt ctggatgacg cgctggcaat caaaaacagc
    39361 gaaatcgccg aagaactgga attgccgccg gtaaaaatcc actgctccat cttggctgaa
    39421 gatgcggtaa aagcggccgt tgccgactac cgcaaacgtc aggaaaacaa ataaagccct
    39481 tcagacggca tcgtcccaca atgccgtccg aaccgcccgc cgcttcggat ccgtccgggg
    39541 cggtacaaca aggaagaaat atgattaccc ttaccgagaa tgccgcaaaa cacatcaatg
    39601 actatctcgc caaacgcggc aaaggcttgg gcgtacgctt gggtgtaaaa accagcggct
    39661 gctcggggat ggcgtacaac cttgaatttg tcgatgaagc caacggcgac gacctgattt
    39721 tcgaaggaca cggcgcgcgc atttatatcg acccgaaaag cttggtttat ctggacggca
    39781 cacaagtcga ttacaccaaa gaagatttgc aggaaggttt caaatttgaa aaccccaatg
    39841 tcaaagactc ctgcggctgc ggcgagagct tccacgttta aggcataaaa acggcgggac
    39901 agtatcaaaa ccgtcccgcc gttttttcgc ttcctgcctg ttatagctgc ctttgccttt
    39961 ccttttccgt tccaccttgt gccggaacaa atcggatttt actaaggctt ttaaagcatt
    40021 gtcgcgtatt ttgcctttat tgtgctgcgc tttgccgccc atattcagtc ctttcgttta
    40081 agaagccgca gattataagg caaaaacagt tttctgccaa aatcttacat ttatcatcct
    40141 actatgtccc aatatttcac cctcttccgg attgaacccg ctttcgatat cggcaccgaa
    40201 aacttggaac aaacctaccg cgccttggcc gcccgtttcc atcccgacaa attcgcttcc
    40261 gcttccgctt tcgagcagaa acaggcggtt atgatgtctt ccaccatcaa cgatgcctac
    40321 cgcaccttga aaaatcccat cgaccgcgcc gcgtacctgc tgaaaacatc gggtatcgat
    40381 gccgacgcgc cggaacatac ctctttcgcc cccgacttcc tcatgcagca aatggaatgg
    40441 cgcgaaacgc tgatggaggc acgggcaggc aacaaccttg aatccttgaa aaatctcgat
    40501 aacgaaatcc gcgccgaaca agaaaaactg ttctgcggtc tgaaacagtc attcgcgcgc
    40561 caagactgcg acaccgccgc acaacaagtc cgccaaggca ggtttctcga caaactccgc
    40621 cacgaaattt cctccgcatt ataatccgca ccgtgtttca gacggcataa ccgccgcacc
    40681 gtttcatgtc gaaatatgct aaaataagca acaatttttt gccatacgaa acattgaaac
    40741 catgaccgac gcaaccatcc gccacgacca caaattcgcc ctcgaaaccc tgcccgtcag
    40801 ccttgaagac gaaatgcgca aaagctatct cgactacgcc atgagcgtca ttgtcgggcg
    40861 cgcgctgccg gacgttcgcg acggcctaaa gccggtgcac cggcgcgtac tgtacgcgat
    40921 gcacgagctg aaaaataact ggaatgccgc ctacaaaaaa tcggcgcgca tcgtcggcga
    40981 cgtcatcggt aaataccacc cccacggcga tttcgcagtt tacgccacca tcgtccgtat
    41041 ggcgcaaaat ttcgctatgc gttatgtgct gatagacgga cagggcaact tcggatcggt
    41101 ggacgggctt gccgccgcag ccatgcgcta taccgaaatc cgcatggcga aaatctcaca
    41161 tgaaatgctg gcagacattg aggaagaaac cgttaatttc ggcccgaact acgacggtag
    41221 cgaacacgag ccgcttgtac tgccgacccg tttccccaca ctgctcgtca acggctcgtc
    41281 cggtatcgcc gtcggtatgg cgaccaacat cccgccgcac aacctcaccg acaccatcaa
    41341 cgcctgtctg cgtcttttgg acgaacccaa aaccgaaatc gacgaactga tcgacattat
    41401 ccaagccccc gacttcccga ccggggcaac catctacggc ttgggcggcg tgcgcgaagg
    41461 ctataaaaca ggccgcggcc gcgttgttat gcgcggtaag acccatatcg aacccatagg
    41521 caaaaacggc gaacgcgaag ccatcgttat cgacgaaatc ccctatcagg tcaacaaagc
    41581 caagttggtc gagaaaatcg gcgatttggt tcgggaaaaa acactggaag gcatttccga
    41641 gctccgcgac gaatccgaca aatccggtat gcgcgtcgtt atcgagctga aacgcaacga
    41701 aaatgccgaa gtcgtcttaa accaactcta caaactgact ccgctgcaag acagtttcgg
    41761 catcaatatg gtggttttgg tcgacggaca accgcgcctg ttaaacctga aacagattct
    41821 ctccgaattc ctgcgccacc gccgcgaagt cgttacccga cgtacgcttt tccggctgaa
    41881 gaaggcacgc catgaagggc atatcgccga aggcaaagcc gtcgcactgt ccaatatcga
    41941 tgaaatcatc aagctcatca aagaatcgcc caacgcggcc gaggccaaag aaaaactgct
    42001 tgcgcgccct tggcgcagca gcctcgttga agaaatgctg acgcgttccg gtctggattt
    42061 ggaaatgatg cgtccggaag gattggctgc aaacattggt ctgaaaaaac aaggttatta
    42121 cctgagcgag attcaggcag atgctatttt acgcatgagc ctgcgaaacc tgaccggcct
    42181 cgatcagaaa gaaattatcg aaagctacaa aaacctgatg ggtaaaatca tcgactttgt
    42241 ggatatcctc tccaaacccg aacgcattac ccaaatcatc cgtgacgaac tggaagaaat
    42301 caaaaccaac tatggcgacg aacgccgcag cgaaatcaac ccgttcggcg gcgacattgc
    42361 cgatgaagac ctgattccgc aacgcgaaat ggtcgtgacc ctgacccacg gcggctatat
    42421 aaaaacccag ccgaccaccg actatcaggc tcagcgtcgc ggcgggcgcg gcaaacaggc
    42481 ggctgccacc aaagacgaag actttatcga aaccctgttt gttgccaaca cgcatgacta
    42541 tttgatgtgt tttaccaacc tcggcaagtg ccactggatt aaggtttaca aactgcccga
    42601 aggcggacgc aacagccgcg gccgtccgat taacaacgtc atccagctgg aagaaggcga
    42661 aaaagtcagc gcgattctgg cagtacgcga gtttcccgaa gaccaatacg tcttcttcgc
    42721 caccgcgcag ggaatggtga aaaaagtcca actttccgcc tttaaaaacg tccgcgccca
    42781 aggcattaaa gccatcgcac tcaaagaagg cgactacctc gtcggcgctg cgcaaacagg
    42841 cggtgcggac gacattatgt tgttctccaa cttgggcaaa gccatccgct tcaacgaata
    42901 ctgggaaaaa tccggcaacg acgaagcgga agatgccgac atcgaaaccg agatttcaga
    42961 cgacctcgaa gacgaaaccg ccgacaacga aaacaccctg ccaagcggca aaaacggcgt
    43021 gcgtccgtcc ggtcgcggca gcggcggttt gcgcggtatg cgcctgcctg ccgacggcaa
    43081 aatcgtcagc ctgattacct tcgcccctga aaccgaagaa agcggtttgc aagttttaac
    43141 cgccaccgcc aacggatacg gaaaacgcac cccgattgcc gattacagcc gcaaaaacaa
    43201 aggcgggcaa ggcagtattg ccattaacac cggcgagcgc aacggcgatt tggtcgccgc
    43261 aaccttggtc ggcgaaaccg acgatttgat gctgattacc agcggcggcg tgcttatccg
    43321 taccaaagtc gaacaaatcc gcgaaaccgg ccgcgccgca gcaggcgtga aactgattaa
    43381 cttggacgaa ggcgaaacct tggtatcgct ggaacgtgtt gccgaagacg aatccgaact
    43441 ctccggcgct tctgtaattt ccaatgtaac cgaaccggaa gccgagaact gaaaatcatc
    43501 tcccgatgcc gtctgaagat tcagacggca tttatttttc cctcatccgt catccagctt
    43561 ctcacaatat agcggattaa caaaaatcag gataaggcga cgaagccgca gacagtacaa
    43621 atatagtgga ttaaatttaa actagtacag cgttgcctcg ccttgccgta ctatagtcaa
    43681 ttaaaaacaa ggg
//
LOCUS       DAWTCI010000006        39928 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWTCI010000006 DAWTCI010000000
VERSION     DAWTCI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39928
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.006"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            101..1282
                     /locus_tag="WM481_000376"
                     /pseudo
     CDS             101..1282
                     /locus_tag="WM481_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            1319..1867
                     /locus_tag="WM481_000377"
                     /pseudo
     CDS             1319..1867
                     /locus_tag="WM481_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1971..2639
                     /gene="hda"
                     /locus_tag="WM481_000378"
     CDS             1971..2639
                     /gene="hda"
                     /locus_tag="WM481_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFA9079778.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHDPFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     NMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            2636..3304
                     /locus_tag="WM481_000379"
     CDS             2636..3304
                     /locus_tag="WM481_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9079779.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKLHLAPLARYSKEELAEFHREFIAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGIANMIGTQLETGPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(3895..4791)
                     /gene="argB"
                     /locus_tag="WM481_000380"
     CDS             complement(3895..4791)
                     /gene="argB"
                     /locus_tag="WM481_000380"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFA9079780.1"
                     /translation="MEFENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKETMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLVDTPEQNSVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDGLIADGTLYGGMLPKIASAVEAAANGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGRGEDA"
     gene            4961..6091
                     /locus_tag="WM481_000381"
     CDS             4961..6091
                     /locus_tag="WM481_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HFA9079781.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDMKI
                     WITARQHPGETMAEWFVEGLLGRLLDSQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVRGKMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALEAQFKTALLNASPDFQDEYGYEKDAPGKANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            6165..7016
                     /locus_tag="WM481_000382"
     CDS             6165..7016
                     /locus_tag="WM481_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFA9079782.1"
                     /translation="MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFSRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRITDINAFWA
                     MGFPQAHYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKP
                     RPTGQTAALFLGGYGVFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(7101..7763)
                     /locus_tag="WM481_000383"
     CDS             complement(7101..7763)
                     /locus_tag="WM481_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HFA9079783.1"
                     /translation="MDSRKFTEASKRRLGELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLAPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYDDGEG
                     GSDFYTWCNAYLYGLDIVPTDWFEAADDEAFEELFYPVMALGGIYDEEENGAIRLQFT
                     EGELAELASELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            7797..7903
                     /locus_tag="WM481_000384"
                     /pseudo
     CDS             7797..7903
                     /locus_tag="WM481_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7962..9755)
                     /locus_tag="WM481_000385"
     CDS             complement(7962..9755)
                     /locus_tag="WM481_000385"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFA9079784.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKRHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGADIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGKAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSQIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNAESYKFISQKISTETGEEPRADIVFGIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVANGQGADTD
                     ILAATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            10001..11110
                     /gene="proB"
                     /locus_tag="WM481_000386"
     CDS             10001..11110
                     /gene="proB"
                     /locus_tag="WM481_000386"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFA9079785.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAIPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIAAESGVPVYICSSLKPDSLAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            11145..12386
                     /locus_tag="WM481_000387"
     CDS             11145..12386
                     /locus_tag="WM481_000387"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFA9079786.1"
                     /translation="MPLPKRQKKSVNTADAEEKNRALLAMADSLEAAAEDILAANRLD
                     LKAAAGKIPDSMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPNGLEIVKK
                     RVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKTGLAQT
                     RIDPEAVQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIETGTGI
                     VHIYIDKDADWDKALRIVYNAKTGRPSVCNSMEVLLVHEGIAADFLPKLERLLVRGRI
                     EAGLPPVRFRLDPQAARYIGGEAAGADDFDTEFLDYILAVKTVASVEEAVGHIEARGT
                     HHSDGIVTENRHAADYFTTHIDSAAVYINASTRFTDGGEFGLGCEMGISTQKLHARGP
                     MGLKELTSYKYIVQGTGQVRE"
     gene            complement(12562..15606)
                     /locus_tag="WM481_000388"
     CDS             complement(12562..15606)
                     /locus_tag="WM481_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFA9079787.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPEFSLMLFHAVKTAVYWLFVGAVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMQSESKTSPVRPVFKEITLEEATRALSSAALRETKKRYIDAFEKNGTAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQEKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPEVAVPEIDILPPPPVSEIYNRTYEPPAGFEQAQRSRIAETDHLAADVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFEDVPSERPSCRVSDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGITHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            15771..16451
                     /locus_tag="WM481_000389"
     CDS             15771..16451
                     /locus_tag="WM481_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079788.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASVGGGQLAQMNYHLLAVNLVTLMVDTSAALWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     IGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSERGIWL"
     gene            16480..16839
                     /locus_tag="WM481_000390"
     CDS             16480..16839
                     /locus_tag="WM481_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HFA9079789.1"
                     /translation="MLSNILPLSIGAIFGTTARWLLNLAVPASLSPATGNLFANWTGA
                     LLIGIFAETVSHPQWKLLLITGFFGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            16917..17023
                     /locus_tag="WM481_000391"
                     /pseudo
     CDS             16917..17023
                     /locus_tag="WM481_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(17444..17980)
                     /locus_tag="WM481_000392"
     CDS             complement(17444..17980)
                     /locus_tag="WM481_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFA9079790.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLSNDEDEITETVLMSKDEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(18014..18370)
                     /gene="folB"
                     /locus_tag="WM481_000393"
     CDS             complement(18014..18370)
                     /gene="folB"
                     /locus_tag="WM481_000393"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFA9079791.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVHVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            18426..19028
                     /gene="plsY"
                     /locus_tag="WM481_000394"
     CDS             18426..19028
                     /gene="plsY"
                     /locus_tag="WM481_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFA9079792.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALVATTAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            19120..19965
                     /locus_tag="WM481_000395"
     CDS             19120..19965
                     /locus_tag="WM481_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFA9079793.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGLYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLIRIGDKLRLFELHPADFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     VILIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLYAELHVHAPKTDGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DNKIR"
     gene            complement(20074..20406)
                     /locus_tag="WM481_000396"
     CDS             complement(20074..20406)
                     /locus_tag="WM481_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079794.1"
                     /translation="MKNLKNISVVAVCAVLLAACASENSVANYAIGDDSAVIKAGRNR
                     AEARISRAELAQHRRQRKNVSEELALEREKRANKHDAIRQGMGTAAGGLMLLNGVVGT
                     VGVMKSVF"
     gene            complement(20456..20704)
                     /locus_tag="WM481_000397"
     CDS             complement(20456..20704)
                     /locus_tag="WM481_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079795.1"
                     /translation="MKMKKLILLSVAAMLVTACTYADRRFVTQESAAEIQAKSRAIQI
                     SERAERAEYRKERREEMMDAARAIKKANENSPNIYFIR"
     gene            complement(20761..20922)
                     /locus_tag="WM481_000398"
     CDS             complement(20761..20922)
                     /locus_tag="WM481_000398"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079796.1"
                     /translation="MPSERRQIAAGRSFGAFRRISRGIVVEKNGAGQVGTRQADREVQ
                     DAKFIFKKY"
     gene            20947..21921
                     /locus_tag="WM481_000399"
     CDS             20947..21921
                     /locus_tag="WM481_000399"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFA9079797.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILAKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYVFTLNAENEFVLTKENPKVPESTKEFAINMSNRRHWLPPVQQYVDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYLQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNAYQAMLDIQPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(22080..22298)
                     /locus_tag="WM481_000400"
     CDS             complement(22080..22298)
                     /locus_tag="WM481_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFA9079798.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLANNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
     gene            22492..22755
                     /locus_tag="WM481_000401"
     CDS             22492..22755
                     /locus_tag="WM481_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HFA9079799.1"
                     /translation="MKGTPQFPQCGFSARAVEVPTQIGRPFAFVNILENPEIRSTLPL
                     IANWPTFPRLWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            22923..24691
                     /gene="ggt"
                     /locus_tag="WM481_000402"
                     /pseudo
     CDS             22923..24691
                     /gene="ggt"
                     /locus_tag="WM481_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            24755..24919
                     /locus_tag="WM481_000403"
     CDS             24755..24919
                     /locus_tag="WM481_000403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079800.1"
                     /translation="MSNQTKIQAIKQASEQILTICETPNTALQAIHLILRHGGAGRIV
                     LASGVSAGYG"
     gene            24864..25106
                     /locus_tag="WM481_000404"
     CDS             24864..25106
                     /locus_tag="WM481_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079801.1"
                     /translation="MAGQEELSWQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLP
                     LISLVLNKGDETLKTGMLNKLPDNAKENLRIMGYLP"
     gene            25173..26423
                     /locus_tag="WM481_000405"
     CDS             25173..26423
                     /locus_tag="WM481_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFA9079802.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDRRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPAKRYYGGCEYVDIVEQLAIDRVKELFGAAYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAVMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNETDARVLSNLVADVLANPE
                     DEANLAKVRGQVTALCDKYPVYGN"
     gene            complement(26496..26885)
                     /locus_tag="WM481_000406"
     CDS             complement(26496..26885)
                     /locus_tag="WM481_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9079803.1"
                     /translation="MEVHDKIRTLREVNQWTQEEMAEKLEMSVNGYSKIERGKSGINL
                     DKLRQIAQIFNIDVVELLAEQNRSFFFSIGDNTNNHHNIIGSDEMLVFENEKLRSLLD
                     AKDELIRQKDSEIAVLKKLVILLEEKK"
     gene            27825..28616
                     /locus_tag="WM481_000407"
     CDS             27825..28616
                     /locus_tag="WM481_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFA9079804.1"
                     /translation="MKKALLALTIAAISGTAMAQLPDFLGKGEYTVRTDISKQTLKNA
                     DLKEKHKVQKNIGFRADMPFDDIHHGMRFEVSHSRDKKDMYVVTESTTKPFGKDVKEK
                     RTDVYAGYTYTQPLSEATKLRAGLGLGYEKYKDAVANEKGTVSTEREAFYTKAHADLT
                     SDLGGGWYLNPWAEVKVDLDAKLKHNATVAGASADINAKTRGWGVGVGANIGKQITDT
                     VGIEAGPFYKHRHFKASGSFVLDGGNIRVDPTKINEYGVRVGVKF"
     gene            28767..29411
                     /locus_tag="WM481_000408"
     CDS             28767..29411
                     /locus_tag="WM481_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9079805.1"
                     /translation="MLASVIDFILHIDQHLLSLSAQYGVWIYAILFLIVFCETGLIVT
                     PFLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRCEYLGKTRRFYEKHGGKTIIIARFVPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGIIEIARAKLAAKSER"
     gene            29430..31340
                     /locus_tag="WM481_000409"
     CDS             29430..31340
                     /locus_tag="WM481_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9079806.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILTGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEICDLLRRYH
                     HVSHELENGSSELLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGKKSGKIIAELEHASFAYDDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGQGRLKEYIGGYQDYIDAKSRENKIQTASAPKASDVEPAKEKPKANRTVKLSYKE
                     QRELDALPDEIAALETEQAEINAQLSDPGIFKDYEKAGALQNRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            31362..31728
                     /locus_tag="WM481_000410"
                     /pseudo
     CDS             31362..31728
                     /locus_tag="WM481_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31840..33315
                     /locus_tag="WM481_000411"
     CDS             31840..33315
                     /locus_tag="WM481_000411"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFA9079807.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKAGGKHVDVYQNGEIVEQYDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETVYNFEHFAHRLKNTAKANPLGTPDILLMLSQEL
                     AVIDNLSGKIHLIVYADPSQPDSYERARERLEDIRTQLRQSCAIPLSLGSKQTQAVSE
                     FGEEPFKACVDKIKDYIFAGDCMQVVPSQRMSMEFTDNPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGCLKEGVTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEIEPEKRGIYGGAVGVWGFNNDMDLAIAIRTAVIKNNTL
                     FIQSGAGVVADSDPTSEWQETQNKARAVVRAAQMVQEGLDK"
     gene            33399..34337
                     /locus_tag="WM481_000412"
     CDS             33399..34337
                     /locus_tag="WM481_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9079808.1"
                     /translation="MQFTSLEICAGAGGQALGLERAGFSHVALIEIEPSACQTLRLNR
                     PDWNVIEGDVRLFQGEGYDGIDLLAGGVPCPPFSKAGKQLGKDDERDLFPEAIRLAKE
                     TDPKAIMLENVRGLLDPKFENYRNHITEQFAKLGYLGQWKLLYAADYGVSQLRPRVLF
                     VALKNEYTNFFKWPEPNSEQPKTVGELLFDLMSENNWQGAHNWRLKAAQIAPTLVGGS
                     KKHGGADLGPTRSKRAWAELGVDGSGLWDSAPPEDFSGMPRLTVRMTARIQGFPDNWQ
                     FFGKKTPMYRQIGNAFPPPVAEAVGRQIIKALKKEN"
     gene            34338..35198
                     /locus_tag="WM481_000413"
     CDS             34338..35198
                     /locus_tag="WM481_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme NgoMIV"
                     /protein_id="HFA9079809.1"
                     /translation="MNPLFTQERRIFHKKLLDGNILATNNRGVVSNADGSNTRSFNIA
                     KGIADLLHSETVSERLPGQTSGNAFEAICSEFVQSAFEKLQHIRPGDWNVKQVGSRNR
                     LEIARYQQYAHLTALAKAAEENPELAAALGSDYTITPDIIVTRNLIADAEINRNEFLV
                     DENIATYASLRAGNGNMPLLHASISCKWTIRSDRAQNARSEGLNLVRNRKGRLPHIVV
                     VTAEPTPSRISSIALGTGEIDCVYHFALYELEQILQSLNYEDALDLFYIMVNGKRLKD
                     ISDLPLDLAV"
     gene            35215..36351
                     /locus_tag="WM481_000414"
     CDS             35215..36351
                     /locus_tag="WM481_000414"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFA9079810.1"
                     /translation="MNTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFDDRAALDELAKCAAVTTEFENVNADAMRSLAKHTNVSPSGDCVS
                     IAQNRIQEKARIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTLDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQTAQRLADELDYVGVLAVEMFVVGDTHELLVNETAPRTHNS
                     GHHTIDACAADQFQQQVRIMCNLPPADTKLLSPCCMANILGDVWQEDGGEPDWLPLQS
                     RPNAHLHLYGKKTAQKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            36398..36880
                     /locus_tag="WM481_000415"
     CDS             36398..36880
                     /locus_tag="WM481_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9079811.1"
                     /translation="MSLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LRQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            37033..38034
                     /locus_tag="WM481_000416"
     CDS             37033..38034
                     /locus_tag="WM481_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9079812.1"
                     /translation="MQPRSRFKPSVRTKCPGQYRIRRKKHYPAQCLIRCDTVFLQRIQ
                     PLIYQNIPIRTPRHIRQHPTIPRRLQQTGIIRSQRPSSRCRNHEPIFRHRPARKKGLV
                     EKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGANIVIAKTSGNGRYAFL
                     GAYGYGLKANNGNEQEAQKLVASILKNTPVFENGGRAAATTFTQRNIGDVLITFENEA
                     NYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQKTARAYLEYLWSEPAQEL
                     AASLYLRPRNPEVLARHKADFPDLDTFPPEEKFGGWDNIMKTYFADGGVFDRLTAQK"
     gene            38614..39219
                     /locus_tag="WM481_000417"
     CDS             38614..39219
                     /locus_tag="WM481_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFA9079813.1"
                     /translation="MNTDFQKILKQLGKQAKKEAAEKQEAEKNKQKQEYDLDFSQAVG
                     QVSPLKNRQQYYAQSDKTPIKARPKDNRTDEENYFYIGSTYNDPPASFCKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYVEPREGNDGCVRILLKRKLRQQD"
     gene            39282..39644
                     /gene="ssrA"
                     /locus_tag="WM481_000418"
     tmRNA           39282..39644
                     /gene="ssrA"
                     /locus_tag="WM481_000418"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT        10142 a        11159 c         9380 g         9247 t
ORIGIN      
        1 caagcaatat gccctgaccg gcaaaacttc cccaaacttc atcaaaaaat caactgtacc
       61 cgtctttttc agacggcatt gataccgtaa gagatttccc atgcaaaacc ggcaaaccga
      121 gctttattcc acgccgtctt ggctgttgca gaccttattg atgattaccg ccgcctcggc
      181 ggtgattctg tttttcgcgc gcaacacgcg cttggggcgg gagtttgcct atatcctgcg
      241 cctttgcctg acaccgaaaa gcacggtcaa agtattgctg ctgattacgg caatggttac
      301 gctgctgtta accgaagtgc ggttgaatgt gttgagtacc ttcatgtcca aaggacttta
      361 cgattcgatg caggatttga atgcttcggc attttggatg tttgcagcga tgaacgccgg
      421 cgtggtactg atacgggcgt ttaacaacgt cgttaacgac tttctcgatt aaggcttggc
      481 gattaaatgg tcggaacggc tcaatgaagt tttgacttcg cgctggcttg ccgacaaaaa
      541 ctactaccgc ctgcaaatgc gccgccacgc gccggacaac atcgaccagc gtatccaaca
      601 ggatgcgcag gaattcatcg cttcgaccat cgaatttgtg cgcggcatgg tcaattcggt
      661 cgttacttct ctggaatttg ccgttgtttt gtggggcttg gcaggcatcc tgaccgtgtt
      721 cggcttcgac attccgcacg gcattgtttg gtttgtcttt attttcatga ttttggcgat
      781 ctttatcgcc atgtggatag gcaacccttt gattcgttac aattatgaaa acaaaaaact
      841 caacggcgac taccgttatt ccctcatcct cgtacgcgac cacgccgaaa gcgtggcgtt
      901 ttacagcggc gaacaacacg aacacggccg gctttccgac cgctttaaag ccattatccg
      961 caaccgttgg cgcatcgcgc ggcaaagcgt ctgcttaagc ggctttaacg atatgttcac
     1021 caacggcatc aaactcttcc tgattatttt gcaagctccg cgcctgtttg ccggacaaat
     1081 caaaatcggc gacatccagc agaccgtcca agccttcgcg cgactgcaaa acgcgctgtc
     1141 tttcttccga atgttctaca acaaattcac cgcctgccac gcccgactgg agcgtctgta
     1201 cgtctttttg ctgagtaccg aagaacaaca cggcgcgcag caacccgaga ttagcgaagt
     1261 ttcagacggc attgcactgg aaaacgtcgc cctgttccgc cacaacggcg aagttttatt
     1321 ggacggcatc aacatcaaac tcaaaagcgg cgattccctg ctgatacgcg gcccgagcgg
     1381 tttaggcaaa acctcgctgc tgcgcgcgct ggcggggctt tggccgttcg gcagcagcgg
     1441 caaagtcagc cgtccgccgc atcaaggcat cctcttcctg ccgcaacgcc cgtacacggc
     1501 acaaggcagc ctgcgcgacg cggtttgtta ccccgatatc gacaaacagc atcccgagtt
     1561 agccgaagcc atgaacacct gccgcttggg ttatctgatt gacaaattgg ataaaacaga
     1621 cggctggcag cacaaactct cccccggcga actgcaacgc gtcgccttcg tccgcgccct
     1681 gctctccaag cccaaaatcg tcctgctcga cgaagccgcc gccgccttgg acgaaccggc
     1741 cgaagcctag ctctaccgcg ccttaaagca aaaactgccc gacagcatca tcagtatcgg
     1801 acaccgaagt acgctcaacg cgttccgcaa tatgcacctc gatgtcggca acgctgcctg
     1861 cggctgaacg atgccgtctg aagccgtttt cagacggcat cgttcaaatt ggaaagacac
     1921 gcgaaaacaa cgtacaataa acccgattcc aacccatccg gtatgccttt gtgaaccagc
     1981 ttattttcga ctttgccgca cacgactatc cgagtttcga caaattcctc ggcacggaaa
     2041 atgccgaact ggtctatgtg ttacagcata aacacgaccc gtttatctac gtctggggcg
     2101 aagaaggcgc gggcaaaagc catctgttgc aggcgtgggt cgcacaggcg ctcgaagccg
     2161 gcaaaaacgc cgcctatatc gatgccgcct ccatgccgct gaccgatgcc gcgtttgagg
     2221 cagaatacct cgccgtcgat caagtcgaaa aactgggcaa cgaagaacaa gccctgcttt
     2281 tttccatctt caaccgcttc cgcaacagcg gcaaaggctt tttgctgctc ggttcggaat
     2341 acacgcccca gcagctcgtc atccgcgaag atttgcggac gcgtatggca tactgcctcg
     2401 tttacgaagt caagccacta accgaccaag aaaaaatcga cgcgctcgcc aatatggcgg
     2461 cggcacgcca agtaaccgtc gattccgaaa ttttcgaata cctgctcaaa cactggcggc
     2521 gcgatatgga cagcctgatg atgatgctcg acacgctgga caactacgcc gtaaccatgg
     2581 gcaaacgcat caccctgccg ctgcttcgcc agcttttgaa acaacaggaa acccaatgaa
     2641 aaacctcgcc atcttcgacc tcgacaacac cctcatcaac accgattccg accacgcctg
     2701 gccgcaatac ctcatcaaaa aagggttggt tgacgctgcc gaaaccgaag cgcaaaacga
     2761 aaaattctac cgcgactacc aaaacggctg cctcgacatc gacgcattcc tcaaactcca
     2821 cctcgccccg ctcgcccgtt acagcaaaga agagctggcg gagtttcacc gcgaatttat
     2881 agcggaatac atcatccccc acatctcgcc tatgcagcgt atgctggtgc agagccacca
     2941 aatggcaggc gacgaaaccc tcgtgatttc ctcaaccaac gagttcatca tcacccccgt
     3001 ctgccgcctt ttcggcatcg ccaacatgat cggcacacaa ctcgaaaccg gtcccgacgg
     3061 ccgctacacc ggcaattaca tcggcacgcc cagcctcaaa gaaggcaaaa tcacccgtct
     3121 gaaccaatgg ctcgccgaac gcggcgaaac gctcgaaagc tacggcaaaa cctattttta
     3181 cagcgactcc aaaaacgacc tgccgctgct gcgcctcgtc agcgaacccg tcgccgtcaa
     3241 ccccgatgcc gaactggaaa aagaagccaa agaaaaaggc tggccggttt tgaatttcaa
     3301 atgacgcaat gccgtctgaa gcccgattcg gcgttcagac ggcattgagg ctgaaatccc
     3361 aaaaacccaa agccacagga attcatccgg aaaaacaaaa aacacaggaa ttcatcggga
     3421 aaaacaacaa tctttccacc gtcattccca cgaaagtggg aatcttgatc cgtccgcacg
     3481 gaaacttata tttcgtcatt ccaccaatcc cacaccacct ttaccggttc aaatccgcac
     3541 aaaaatccac atcccgtcat tcccacgaaa gtgggaatct aggacgtaaa atctaaagaa
     3601 accttttttt cccgataagt ttccgtgccg acaggtctgg attcccgcct gcgcgggaat
     3661 gacgaaattt caaagttatg gcgttatcgg aaaaacagaa aatcaaagcc ggagaattta
     3721 tccaaaacaa ccggatttca aaaaaaccaa attcccggcg ggaatgacgg attttaggtt
     3781 tctgttgtgg tttctgtttg ctcgggaata acaacttaga aattgcccga aaccccaaaa
     3841 acagaaaccg gacaaacagg ttttccgcca aagccggcat tttccgactt tgcttcaggc
     3901 atcttcccct ctgcctaaaa tcatcgaccc gataccggca tcggtaaaga tttccagcaa
     3961 aagcgcgttg ggcaacctgc cgtcgatgat gtgcgtggct ttcacaccgt tggcggcggc
     4021 ttcgaccgca gaagcgattt tcggcagcat accgccatac agcgtgccgt cggcaatcag
     4081 cccatcaatc cgtttcggcg tgagtttggt cagcagattg cccgttttgt ccatcacacc
     4141 ggcgatattc gtcatcatca agagtttttc ggcgttcaat tcttccgcca atttccctgc
     4201 caccaaatcg gcgttgatgt tgaacgcttc gcctttttca cctacgccga cgggggcgac
     4261 gacgggaatg cagccgcgtt ctatcagccc tttaaccaaa ccggtatcga tgctttccac
     4321 cgtaccgacc tgtccgatgt ccacgctatt ctgttcgggc gtatcgacca aaagtttctt
     4381 cgccttaatg aaatggtcgt cgcgcccgct cacgccgacc gcgtgccctc catatgtgtt
     4441 aatcatcgac acgatttcct tgttgacgtg cccgcccaat accatttcga caatatccat
     4501 cgtctctttg tcggtaacgc gcattccttg gacaaattcg ccctttttgc cgactttttc
     4561 aagcatcgca ttgatctgcg gcccgccgcc gtgaacgatg acgggatgaa tgccgaccag
     4621 cttcagcagc acgacatcgc gggcaaaccc ttctttcaag gcaggttcgg tcatcgcgtt
     4681 gccgccatac ttgatgacgg cgaccgaacc ggaaaaccgg cggatgtaag gcagtgcttc
     4741 ggcaaggata cgcgccttgt cggcggcgga aataatgttt tcaaactcca taacggctcc
     4801 atattgttga caatttcggc ttatgttaac gccaaatgcc gtctgaaaca atcttcagac
     4861 ggcgtttggc acaccttcgg gcaggttgaa gccttgcgat acgggcggta aaatcgcggt
     4921 ttatccggtt cgagaaaccg gcgacagcaa ggactttccc atgattaaaa tcagcacccg
     4981 cttcgatgcc ggctcggtcg tcgtcaaaga cctgaccgac ccttccaaca tccgcctcgc
     5041 cctgcgtccc gacaacgcct ccgatttcgc acaatggttc tacttccggc tgcaaggcgc
     5101 ggcctatcaa aactgcatca tgcattttga aaacgcggca gaagccgcct acccgaaagg
     5161 ctgggaaggc tatcaggcgt gtgcctcata cgaccgccgc aactggttcc gcgtaccgac
     5221 ttcctacgaa aacggcgtac tgaccgtcaa tcatacgccg ctgtccaaca gcgtgtatta
     5281 cgcctatttc gaaccctatt ccgaagagca gcacctcaac ctcctcggcg acgcacaagg
     5341 cagcggcctg tgccgcatcg acgacttggg cagcaccgtg caaggccgcg acatcaatct
     5401 gctgaccata ggcaaccaag tcgaaagcga catgaaaatc tggattaccg cccgccagca
     5461 cccgggagaa accatggcgg aatggtttgt cgaagggctg ctcggcaggc tgctcgattc
     5521 gcaagacccg acagcacgca ccctgctcga ccgcgccacg ttctacatcg tccccaatat
     5581 gaatcccgac ggttcggcac tgggcaacct tcgtaccaac gccgccggtg cgaacctcaa
     5641 ccgcgaatgg gaaaacccga ctttggaaaa aagccccgaa gtgttcttcg tgcgcggaaa
     5701 aatgctggaa accggcgtgg atttgttttt ggacatccac ggcgatgaag gtctgccctt
     5761 tgtctttgtt gcaggaacgg aaggtgtgcc gaactacaat ccgcgcattt ccgcgttgga
     5821 agcgcagttc aaaaccgccc ttttgaacgc cagccccgat ttccaagacg aatacggtta
     5881 cgaaaaagac gcgccgggca aagcgaatat gaccttggcg accaactggg tcggcaaccg
     5941 tttcaactgc cttgcctaca cgctggaaat gccgttcaaa gacaacgcca acctgcccga
     6001 cgacgacttc ggctggaacg gccagcgttc tttgcggctt ggggaagcgg tgctttccgc
     6061 cattttgaac gtcgccggcg atttgcgctg acactcccca ctgaaaatgc cgtctgaaag
     6121 cccttttgcg tctttcagac ggcatttgaa aacgaaagcc cgttatgatt atccatcacc
     6181 aattcgatcc cgtcctcatc agtatcggcc cgcttgccgt gcgctggtat gccttaagct
     6241 acatcctcgg atttattctt tttacctttc tcggcagaag gcgcatcgcg caaggcttgt
     6301 ccgtttttac caaagaatcg ctcgacgact tcctgacatg gggcattttg ggcgtgattt
     6361 tgggcggacg cttgggctat gtcctgtttt acaaattctc cgactacctc gcccatccgc
     6421 ttgatatttt caaggtatgg gaaggcggaa tgtcgttcca cggcggcttt ttgggtgtag
     6481 ttattgccat atggttgttc agccgcaagc acggcatcgg cttcctcaaa ctgatggaca
     6541 cggtcgcgcc gctcgttccg ctgggtctcg cttcgggacg tatcggcaac tttatcaacg
     6601 gcgaactttg gggacgcatt accgacatta acgcattttg ggcaatgggc ttcccgcaag
     6661 cgcattacga agatgccgaa gccgccgcgc acaatccgct ttgggcagaa tggctgcaac
     6721 aatacggtat gctgccgcgt catccctcgc agctttatca gtttgccctt gaaggcatct
     6781 gcctgttcgc cgtcgtttgg ctgttttcca aaaaaccgcg cccgaccggg cagactgccg
     6841 cgctttttct cggcggctac ggcgtgttcc gctttattgc cgaatttgcg cgccaacccg
     6901 acgactatct cgggctgctg accttggggc tgtcgatggg gcaatggttg agcgtcccga
     6961 tgattgtttt gggtatcgtc ggctttgtcc ggttcggcat gaaaaaacag cactgaccgc
     7021 ctgtgaaaaa taccgtctga aacatggaaa tccggtttca gacggcattt ttacgtttgg
     7081 ttcagatgaa aacaaacgct tcaattctta ccgcaacacg ccttgtattt cctgccgctg
     7141 ccgcacggac agggatcgtt cctgccggtt ttttcccctt ccctgcggac ggtttgcggt
     7201 ttgttgatga ccgcctgcca gtagcggtag atgtctgcca gcgcgtaagg cagttcggac
     7261 gcaagttccg ccagctcgcc ttcggtgaat tgcaggcgga tagcgccgtt ttcctcttcg
     7321 tcgtaaatgc cgcccaatgc catcacgggg taaaacagct cttcaaacgc ttcatcatcg
     7381 gcggcttcaa accaatcggt cggcacaatg tccaaaccgt aaagataggc gttgcaccaa
     7441 gtgtaaaaat cgctgccgcc ctcgccgtcg tcgtagagcc acaaatcggg cagcttttta
     7501 tccgacatcg cggcggttgt ttccatcgcc attgccaaaa ccagccgttc gatttcggaa
     7561 cgttcggcgg cggtaaattg cgattcgtcg cccaacactt cgggcagcca gtcgagcggt
     7621 gccaatttgt ccggcccgct caacagcgcc gtcataaaac cttgaacctc gtcgcaacgc
     7681 atcgtgttgc cttgttcgct tttggcatcc aacaattcgc ccaaccgccg tttggatgct
     7741 tcggtaaatt ttcgggaatc catcattttc cttttcaaat gggttttgcg ccctattata
     7801 gtggattaaa tttaaatcag gacaaggcgg cgagccgcag gcagtacaaa tagtacggca
     7861 aggcgaggca acgccgtacc ggtttaaatt taatccacta tatcgccgca atgccgtctg
     7921 aagccttttt atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgccc
     7981 tgcgccttcg gtttggcggc actaaattcc agcttgctca aagcggaaag gtaagctttg
     8041 gcggtcgccg ccaaaatgtc ggtatccgcg ccctgaccgt tggcgacgcg gttgccgcgc
     8101 gccaaacgga cgctggtttc gccttggctt tccgtacctt gcgtgacggc gttgacggaa
     8161 taaatctgca aagtcgcgcc gctttgcgcc acgctctcaa tcgctttgaa aattgcatca
     8221 acagggccgg agccggttgc ggaagcgcgt ttttcttcgc ctttgatgcc gaacacgatg
     8281 tcggcgcgcg gctcttcgcc ggtttcggtg ctgatttttt gagaaatgaa tttatagctt
     8341 tcggcattca tattgcccat ttcgtccgac accagcgcgt gcaggtcttc atcgaagatt
     8401 tcgcgttttt tgtcggcgag ttctttgaag cgtgcaaatg ccgcgttcag tgcctcttcg
     8461 ctttccaact cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat
     8521 ttgcccaagc tcaatcggtt tgccgaccaa ccgaccgatt cggcagacat aatctcgtaa
     8581 gtttcgcggt gtttcagcac cccgtcctga tggatgcccg attcgtgcga aaaggcattc
     8641 gcgccgacaa tggctttatt gggctgcacg ggatagcccg taatggtgga caccagtttg
     8701 gacgaaggca cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc
     8761 accttcaacg ccatcacgat ttcttcaacc gaagcattcc ccgcgcgttc gcccaaaccg
     8821 ttaacggtac attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc
     8881 gccaagccca aatcgttgtg gcagtgtgcc gaccagacga ctttgccgcc gttgggcgtt
     8941 ttggcaatca gttcgcggaa aaattcttcg gttttgtacg ggatggaata gccgacggta
     9001 tcgggaatat tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg
     9061 aaatcgattt gcgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca
     9121 cgagcgattt ttaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcagc
     9181 ttgtactcca tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg
     9241 ggcgcaacgg ccttacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag
     9301 accgttgatt tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg
     9361 gcggcggcaa aacccgcttc gatgatgtcc gcgcccaatt tttccaactg gcgggcaacg
     9421 cggatttttt cctctttggt catagcggcg ccgggcgatt gttcgccgtc gcgcagggtg
     9481 gtgtcgaaaa taataacgcg gttggcctgt gtcatttctg ttctctccag agattcgttt
     9541 ttttgtaaaa aagcattcaa atccagcggt cttccttgcg attcggcaag cgcggtcaat
     9601 ttttgcagtt gcgccaaagg cagcgagccg cgtgtgcgcc atttgtagat ggcggcggtc
     9661 gtcgcggcat tttcaggatc gtgccgtttc aacgcttcgg caagcgcgtt cacgccgccg
     9721 aaataggcga ccagacggtc tatatctaat tgcatggtag tccttgtcta aaataaatca
     9781 gcccgcagca gaaatatgga caggattata cgcattttgg acaaaacggc aatattaatt
     9841 tgaaaaaatc tcaataagtt gctataaatt ttattaattt tatacatttt gtctatttta
     9901 ttgcagatgc ggcgtgattg ttgacaattg cgacagaaca gggcaaattc ggcggtacaa
     9961 ggcggcaacg agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt
    10021 atttaaagtc ggcacatctt cgattaccca ttcggacggc agcctctcgc gcggcaaaat
    10081 ccaaaccatc acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt
    10141 gtcttccggc gcggttgctg cagggttcgg cgcgctgggt ttcaaaaaac gtccggtcaa
    10201 aatcgccgac aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac
    10261 ggcaaacctg tcttcagacg gcatcgtgtc cgcacaaatc ctgctcagcc gtgccgactt
    10321 tgccgacaaa cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc tgcaacgccg
    10381 cgcgattccc atcatcaatg aaaacgacac ggtttcggtt gaggagttga aaatcggcga
    10441 caacgacaca ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct
    10501 gaccgacata gacggtcttt acaccggcaa cccgaacagc aatcccgatg ccgtacggct
    10561 ggacaaaatc gaacacatca accatgaaat catcgaaatg gcgggcggct cgggttcggc
    10621 aaacggcacg ggcggtatgc tgaccaaaat caaagcggca accatcgccg ccgaatccgg
    10681 cgtaccggtg tatatctgtt cctcactcaa acccgattca ttggccgaag ccgccgaaca
    10741 tcaggcggac ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacac agaagcaatg
    10801 gctggcgttc tattccgaaa gccggggcag cgtttatgtg gacgaaggtg cggaacacgc
    10861 tttgtccgaa caagggaaaa gcctgctgat gtcgggcatt gccggaatcg aagggcattt
    10921 ttcccgtatg gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg
    10981 gcgcgtcctt ttcggctccg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg
    11041 cgtgttcatc catcgggacg attggatttc catcacgccc gaaatacgcc tgcttctgac
    11101 cgaattttag aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc
    11161 ggcaaaaaaa atccgtcaac acggcggatg cggaagaaaa aaaccgcgcg ctgcttgcta
    11221 tggcggacag tctggaagcg gcggcggaag atattttggc ggcaaaccgt ttggatttga
    11281 aagcggcggc aggcaaaatt cccgacagca tgaccgaccg ccttttgttg gacggcaaac
    11341 gcatttgcgc gatggcggac ggcatcaggg cggttgccgc gctgcccgac cccgtgggcg
    11401 aaatactgga aacctcgact ctgcccaacg gcttggaaat cgtcaaaaag cgcgtggcga
    11461 tgggcgttat cggcattatt tacgaaagcc gcccgaacgt tacttccgat gcggcggctt
    11521 tggcactgaa aagcggcagc gcggtcgtac tccgcagcgg caaagatgca tttcaatccg
    11581 cacgcgccat cgttgccgcc ctgaaaacgg ggttggcaca aacccgcatc gaccccgaag
    11641 ccgtacagct catcgaagac acagggcgtg agggcagtta cgaaatgatg agggcgaaag
    11701 attatctaga cctgctgatt ccgcgcggcg gggcagggct gatacgggcg gtggttgaaa
    11761 atgccgtcgt gccggtcatt gaaacgggga cgggcatcgt ccacatttat atcgacaagg
    11821 acgcggattg ggacaaggca ctccgtatcg tgtacaacgc caaaaccggc cgcccgtccg
    11881 tgtgcaactc gatggaagtg ctgctggtgc atgaaggcat tgctgccgac ttcctgccca
    11941 agctcgaacg gctgttggtt cgcggccgta tagaagccgg actgccgccc gtccgcttcc
    12001 gtttggatcc gcaggcggcg cggtatatcg gcggcgaagc ggcgggtgca gacgatttcg
    12061 ataccgagtt tttagactac atcctcgccg tgaaaaccgt cgcttcggtt gaagaggcgg
    12121 tcgggcacat cgaagcccgc ggcacgcacc attcagacgg catcgttacc gaaaaccgcc
    12181 acgctgcgga ctatttcacg acccatatcg attctgccgc cgtgtatatc aacgcgtcca
    12241 cgcgcttcac cgacggcggc gaattcggct tgggttgcga aatgggcatc tccacgcaaa
    12301 aactccacgc ccggggcccg atgggtttga aagagctgac gagttacaaa tacatcgtac
    12361 aaggcacggg acaggttagg gaataatccg aattcaaacg tctgatatag tggattaaat
    12421 ttaaaccggt acggtgttgc ctcgccttgc cgtactattt gtactgtctg cggcttcgcc
    12481 gccttgtcct gatttttgtt aatccactat aaaaatgccg tctgaaacag tcttcagacg
    12541 gcatttctat ttgcaaaaaa atcaagcatt gtccaagggg acgagaatcg tacggttgcc
    12601 gttgtgttcc ggtgcggaca caatgccttc cgcctccatc tggtcaatca gacgcgcggc
    12661 gcggttgtag ccgatgcgca aggcgcgctg tacgcccgaa atgctggctt tgcgcgtttt
    12721 caggacaacg gatacagcct cgtcgtacat cggatcggtt tcgccgtcgc cgctgcgccc
    12781 gataccgggc agctcttcgc tgccgccgcc gctcaaaata tcgtcaacat agtccggctc
    12841 gccaaactgt ttcaaatatt cgaccacgcg gtgcacctct tcatccgagg caaacgcgcc
    12901 gtgaacgcgt tgcggatagg cagtacccgg cggcaggaac agcatatcgc cctgaccgag
    12961 caggttttcc gcgcccattt ggtcgagaat cgtgcggctg tcgattttgc tggacacttg
    13021 gaacgcgata cgcgtcggga tgttcgcctt aatcagaccc gtgatgacat cgacgctggg
    13081 gcgttgtgtg gcaaggataa ggtggatgcc tgccgcgcgg gctttttggg cgaggcgcgc
    13141 aatcagttct tcgattttct tgcctgccgt catcatcaaa tcggcaaact catcgaccac
    13201 gaccacgata aacggcagtt tttccaaagg ttcgggatcg tcgggcgtga ggctgaacgg
    13261 attgccgatt ttttctcccc ttgctgcggc ttcggcgatt ttttggttga agcccgcaag
    13321 attgcgcacg cccataaagc tcatcaggcg gtagcgtttt tccatttcgt taacacacca
    13381 gttcagcgcg tttgccgcca gcttcatatc ggtaacgaca ggggcgagca ggtgcgtgat
    13441 gccttcgtaa atgctcaatt ccagcatttt cggatcgatc ataatcatac gcacgtcttc
    13501 cggcgcggct ttgaaaagca tggacagaat catcgcgttg acacccaccg atttgcccga
    13561 accggtcgtg ccggcaacca gcaaatgcgg tgctttgccc aagtcggtta cgacgggctg
    13621 tccggtgatg tcctgaccga gcgcgagcgt cagcttggat ttggattcgg caaactcggg
    13681 cgaattgaaa atttcgctca ggcgtatcat ttggcgtttc gggttcggaa gttccaaacc
    13741 catgcaggtt ttgccgggga tggtttcgac aacgcggatg gaagccacgc cgagcgaacg
    13801 cgccaagtct ttttccaaat tcagaacgga attgccgcgc acgccgacat cgggttcgat
    13861 ttcataacgc gtaatcacgg ggccggaata agaatcgaca accttgacct tgactttgaa
    13921 ctccgccaat ttttcttcga tggtgatgct gttttccaac agttcttctt cggtttgcgt
    13981 cgcctcggga ttgaacagcg gaggcagaag caggtcggtt gtcggcaggt gttcggatac
    14041 cgcaccggtc tcttccgatt ggaacgcccc ttcatccgct tccgtatccg atacccggca
    14101 ggacgggcgt tcagacggca catcttcaaa cggacaaacc gcctgactgt catgcccgaa
    14161 cgcttcggtt tccgacagat attgcccgct tgaccgctct gccgcaccct cactgccgtc
    14221 atctgcaata gcggcggttt cctcctgcca acctccattc aaaacatcag cggcaagatg
    14281 gtcggtttcg gcaatgcggc tgcgttgcgc ctgctcgaat cctgccggcg gctcataggt
    14341 acggttgtag atttccgata cgggaggcgg cggcagaata tcgatttcgg gtacggctac
    14401 ctccggcact tcgggtggtt cgaccaccca agcatcgggc gcggcaggct cttcaatatg
    14461 gatatcggca gcttcatcat aaaccggacc gccgtatccc acagacgaaa cggtttccgt
    14521 gaaaacattt tcggtttcag acggcatttc gacatcccgt ttcccgacaa ccgtccgact
    14581 ttccggaatc aggcgcgaaa tccgcgcctc cgcagaaacc gttgccttgt tcggttcttt
    14641 caaattgacg gaaacgcggc ggcgggcatc gcggacggct gtgccgtctg aaacggactg
    14701 cccttgggaa acatccgggg attttgcctc cgcctgccct ttctcctgcc ctgcatgacg
    14761 gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag acggatgctg
    14821 cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt ctttgtccgc
    14881 atcaaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga taatctgcag
    14941 cccttccatc ggggtatcgg acacgcgtac tttggggacg gctgttccgt ttttctcaaa
    15001 tgcatcgata tagcgttttt tcgtttccct taaagccgcg ctgcttaaag cacgcgttgc
    15061 ttcttccaaa gtgatttcct taaaaaccgg acggacgggc gaagttttgc tttcagactg
    15121 catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga ttacggcagt
    15181 tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgctgctt ccgtttccgc
    15241 ttcttccgtc ccgtttccac tgtctgaata cccgtcggat gcggtcggaa catccgcacg
    15301 gtttgcagaa gcaggcggaa cgggcctgtc cggttcggat tcgtgcgcca gatagtttcg
    15361 gcagaaacgg acggcaccga caaacagcca atacactgcc gttttgacgg catggaaaag
    15421 catcagggaa aactcgggaa aatcgggcat accgtctttg atttcaggca gctccgcctg
    15481 tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc gttcggattg
    15541 tgcgcggaca aaaaacaggc cggcaagcgc aagcaacaaa ataacgatca aaactatcca
    15601 aaacatatgc cgtttcccgc aagacaaacg attaaccgag tttattttaa cgtgtttcca
    15661 gcccaaaaaa aacaaccggc cggcaattct gccgaaaccg attcggcttt cggtttgccc
    15721 ccgccgacag tataatagcc gccccttccc ttaatctttg cggcattacc atgattttcc
    15781 aaacagtctg gttttcagat gtggtactgt cggtttcgtg gattgtcctt atcctgatac
    15841 tggcagcttc cgcgccgtcg gcattccgtt cgcttgcccg ctaccgttcc gcccttcccc
    15901 tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgtcggc ggcggacagc
    15961 ttgcccagat gaactaccac ctgcttgccg tcaatctggt tacactgatg gtggatacct
    16021 ccgccgccct ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg tttgccggtt
    16081 ctgccggcgc atatccgccg aacgctttgg tgctgatact gcccgcgctt gtcgtcaacc
    16141 gcctgtcgcg tatgctggtc aaccgtctgc cgcccaatat tttcatcttt attttcgtca
    16201 acggctttct cgcttccgcc gccggcattc tgctgaccgg gctggtgctg atcggtattt
    16261 tggatgccgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg cccgtcttta
    16321 ttctgctggc gtgggcggaa gccttcctca gcggcatttc aactgccatt ttcgtggcac
    16381 tgaagcccca ttggatcaac actttcgacg acaaccgcta tctgaagtcc gagcgcggca
    16441 tatggctgta gccttcagac ggcataggca gacaaaacca tgctttccaa catcctcccc
    16501 ctttccatcg gtgcaatatt cggcacaacg gcgcgttggc tgctcaacct cgccgtcccc
    16561 gcatcgttgt ctcccgccac aggcaacctg tttgccaact ggacgggcgc gctgctcatc
    16621 ggcatctttg ccgaaaccgt cagccatccg caatggaagc tgctgctgat taccggtttt
    16681 ttcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct gctccaatcg
    16741 aaccgtcccg cttcggcact tgccaatatt ttcctacaca cggcaggttc gctgctgctg
    16801 acttggctcg ggctgaagat agggacggca gtcaaataac cgcaaaaaat tgcttccttt
    16861 ggttttgaca taatctataa attggcttcg cttgtttaaa tactgcctgt gccaattata
    16921 gtggattaaa tttaaatcag gacaaggcgg cgagccgcag acagtacaga tagtacggca
    16981 aggcgaggca acgccgtacc ggtttaaatt taatccacta taccgttctc aaattcaaat
    17041 tgggctttta ggtctataac aaagaaaaag ccttgggagg ataaacctac caaggctttt
    17101 tcttaaaaaa cgctctatca ctgtgcctaa aaatgcaaaa aagtaaccgt cataatcctt
    17161 ttgccaaaat caatatcatc atccgatgaa tgaacggacg gtatataaga aagcgttaat
    17221 aaactcataa accagtaaga aaaatcagta acgaagatat tcattattac ccattcttaa
    17281 aaattatcac gttcgtttgg gtaattatca tacccttttc cctcttatta ctaaagcacg
    17341 aattacttta aagtacgcac ccagagacaa gccgaaaagg catatccgat ccgccaaaca
    17401 aaatgcccgc cgacttactc cggcgggcgg ttccaacatc ctgtcaatcc ttcatcagcc
    17461 agtattgcaa accgattaat gttttaccgt ctttaatttc atcgtttgcc aatgcctgac
    17521 ggacttcatc tttcgacatc agcacggttt ccgttatttc atcttcatca ttagaaagcg
    17581 tactgcccaa gcgcacgcct tccgcctcga acagatacat tttttcattg caaaaaccga
    17641 ccgccgtata aaaactgtac agcaggcgca cgctgtcggc aacataaggc gtttcctccg
    17701 ccaattctcg cagcgcacac gctgccatat cctcgcccgc cacatccagc ttgcccgcag
    17761 gaagttccaa tgtcgcctga tttgccgcat aacgccactg ccgcaccaaa acgacctttt
    17821 cgtcatccgt taccgccaac acgcacgccg cgcccggatg ccggatgacg atgcgctgcc
    17881 cttcattgcc gttgggcagc ctgaccttat ccctgctgat actgacgaaa ccgccctcgt
    17941 aaatggtttc gccgcctaat tttacttccc tcaaatccac ttcatatctc ctttccgttt
    18001 cctattctgc cgttcaatct tcacgcttgc cgcgctcgat ttccactcca acctcgcgca
    18061 cgccttccaa aatacccggc ttgacgattt tcacgtgcac ccacaccgcg ccgaaatatc
    18121 ccaaaaccaa atcggcaata tattccgcca acgcttccaa aagcaggaaa tcctgttctt
    18181 tcagatgtcg gcgcagcgtt tcgcatacct cggcataatg caccgtattg gcaatatcgt
    18241 cgtccgaacc cgctttctcg ggaacgccga tgtccaaatc gacaatcagg gtctgcaacc
    18301 gttcgcgttc ccagccgtac acgccgataa gcgtatccgc cttcatgccg tgcaaaaaga
    18361 ttttatccat aatccgcccc gttttttgct aaaataaccg caccattcta agtaaagtac
    18421 cgcaaatgtt caacataccg gccgtcgccg tttcctattt aatcggttca ctttctttcg
    18481 ccgtcatcgt ttccaagtat tacgggatgg acgacccgcg cacctacgga tcgggcaatc
    18541 ccggcgcgac caatgtttta cgcagcggca aaaaaaaggc ggccgcgctg acgctcttgg
    18601 gcgatgccgc caaaggtttg gttgccgttt tgcttgcacg cgtgcttcaa gaaccgctcg
    18661 gtttatccga cagcgcaatc gccgccgtcg cactcgccgc gctggtcggg catatgtggc
    18721 cggtgttttt cggatttaag ggcggcaaag gcgtggcaac ggcattgggc gtgcttctgg
    18781 cactctctcc tgcaactgcc ttggtctgcg cgttgatttg gcttgtgatg gcattcggct
    18841 tcaaagtatc ctcccttgcc gcgctggtcg ccacaaccgc cgcccccctt gccgcactgt
    18901 tttttatgcc gcatacttct tggattttcg caaccctcgc aatcgccata ttggtgttgc
    18961 tccgccataa gagcaacatc ctcaacctga ttaaaggcaa agaaagcaaa atcggcgaaa
    19021 aacgctgatt cggcatttgt tttccattac gtcatgccgt ctgaaaccat gttcccgcgt
    19081 tcagacggca tttccgcaca aacggctttc atacatacca tgctcagtta cagacacgca
    19141 tttcacgccg gcaaccacgc cgatatgctc aaacacttca ccttgttttt agtgctgcaa
    19201 tatttcaacc gcaaagacaa gccctactgg tacatcgaca cgcacggcgg cgcgggtttg
    19261 tacaatctcg aaggcagcga ggcgcagaaa gtcggcgaat accggcaagg catcgccctg
    19321 ctccgacagg cgcaaaacct gcctgccgaa ctctctgact ttgccgcgca catacaaaaa
    19381 atcctaccct cgcccgaact ttactgcggc tctccgtggc tggcgcaatc gctgatccgc
    19441 atcggcgaca aattgcgcct gtttgagctg caccctgccg actttgtcca tctgcaaaac
    19501 aatatgggcg aagcgggatt gggcaaacgc ggacaagtgt tgcgcgaaga cggttacaaa
    19561 gggctgattt ccctgctgcc gcccccgccg cgacgcgctg ttattctcat cgacccgccc
    19621 tatgaggaaa aacaggacta ccggcgtgta acggaaacgc tgaaagccgc tttaaaacgg
    19681 tttgagtccg gctgttatct catttggtat ccctgcctca gccgcgaaga aagccgcaag
    19741 ctgccggaag aattgaaaaa actcctgccc gacaactacc tttacgccga actgcacgtc
    19801 cacgcgccga aaaccgacgg tttcggtatg cacggcagcg gaatgttcgt gattaatccc
    19861 ccttaccttt tagccgaaca attggcggca aacctccccg ccctgacacg gctgctggcg
    19921 caagacgaag gcgcgcgcta cctgttggac aacaaaatcc gctgaccccg atgccgtctg
    19981 aaacccttca gacggcatct tccgtcaaac aaacaaaacc gggcgataca cactccatat
    20041 cgtccggttc tgcataaaac cagtattcaa aaatcagaac acgcttttca ttacacccac
    20101 ggtaccaacc acaccgttca gcagcatcag accaccggca gccgtaccca taccttgacg
    20161 gattgcgtca tgcttattgg cacgtttttc acgttccaaa gccaactcct cagaaacatt
    20221 tttacgttga cggcggtgtt gcgccagttc cgcacggcta attcgcgctt cggcacggtt
    20281 acgaccagcc ttgattacgg ccgaatcgtc gccgatagca tagttggcta cagaattttc
    20341 agaagcgcag gcagcaagca aaacggcaca cacagcaaca acggaaatat ttttcaagtt
    20401 tttcatacct gtcctattat ctcaaaataa agaaaaaact aatccaactg gagttttatc
    20461 ggataaaata aatattgggt gaattttcgt ttgccttttt gatggcgcgt gccgcatcca
    20521 tcatttcttc ccggcgttct ttacggtatt cggcacgctc ggcacgctcg cttatctgaa
    20581 tggcacgact tttcgcctgt atttccgctg cagattcttg agttacaaaa cggcggtctg
    20641 cgtaagtaca tgccgtcacc aacatcgcag ccaccgacaa caaaatcagt ttcttcattt
    20701 tcatctacct atatttaagt ttatttgtaa aatatgttat cactatatat atatataatg
    20761 tcaatatttt ttaaaaataa atttggcatc ttgtacttct cgatccgcct gccgcgttcc
    20821 gacttgcccc gcgccgtttt tttccactac aatcccacgc gatatccggc gaaacgcacc
    20881 aaaggaacgc cccgcagcaa tctgccgcct ttcagacggc acaaaccttt ccgaaacgag
    20941 caaaccatgg acacactgac ccgattcctc cccgaacacc tgcaacaaaa ccaactgccc
    21001 gaagcactcg gcggcgtttt gctgtccgtc gtttccgcct gtaccgaaat caacgccaaa
    21061 gtccgcctcg gcgcacttgc cggcgtattg ggtatggcgg gcacgggcaa tattcagggt
    21121 gaagaccaga aaaaactgga tgttatcgcc aacaacatta tgattgacac actcaaagcc
    21181 aaccctgccg ttgccggttt ggcaagcgag gaagaagaca ctttcgtaag tgccggtgaa
    21241 aacggacgct atctcgtcct attcgaccct ttagacggat cgtccaatat tgatgtcaac
    21301 atttccgtcg gtacgatttt ctccattctc gccaaacccg aaggcgcatt ggcaaccgaa
    21361 tcattccttc aaacgggcag acagcagctt gccgcaggct atgtcctcta cgggcctcaa
    21421 acccagctcg tattcacatt cggacacggc gtgtacgtat ttacactcaa tgccgaaaac
    21481 gaatttgtgc tgaccaaaga aaacccgaaa gtacccgaaa gtaccaaaga attcgccatc
    21541 aatatgtcta accgccgcca ctggctaccc cccgttcaac aatacgtcga cgagctcttg
    21601 gcgggcgaaa ccggtacgcg cggcaaaaac tacaatatgc gctgggtagc cagtatggtt
    21661 gccgaaatcc accgcatcct gatgcgcggc ggcgtgttca tgtatctgca agacaaacgc
    21721 gacccgtcca aacccggcaa actgcgcctg atgtacgaag ccaaccctat ggccctgata
    21781 ctcgaacagg cgggcgcatc ggcaagcaac gcatatcaag ccatgctcga tatacagccc
    21841 gaaagcctgc accaacgcgt cgccgtcatt atgggcagca gcgaagaagt ggattacctc
    21901 aaccgtctgc attcaaaata ggcagagacc gtactgataa agaccggcgg cagcgtgaat
    21961 ccgatactgc gccgatattt caagcatacc ctccggaaaa acagaaaact gcctttccga
    22021 acgataaaaa taccgtctga aaaattttca gacggtattt taaaaattaa agctcaaatt
    22081 tatttgcccg ccgctttttg caggatggcg tacaactcgt ctttcagttt caatttggct
    22141 tttttcagct catcgatggt ttccgcaccg ctggtaaccg gattgttggc cagaccggta
    22201 attttatcgt ccagctcgtt atgctcgtcg aacagacggg cgaagcggga attttcctgt
    22261 ttcaatttgg aaatcaaatc acggtattct gggaacataa tcgctttcct ctcttggttt
    22321 acggttaaaa agtcaaattg aatctttaca ttaattataa caaagataat atttttatcc
    22381 agtgttttgt tatacacggc aacaccgtcc gcagaaaacc gccccaccgt ccgataaaat
    22441 cgttgacaat cttccgcttt acgcataaat tttcagccct tcctactgca tatgaaaggc
    22501 acgccccagt ttccccaatg tggattttca gcgcgcgcag tagaggtgcc gacccaaatt
    22561 ggcagaccct ttgcttttgt taatatccta gaaaatccag aaattcgcag tactctacca
    22621 ctcatcgcca attggccaac ctttccacgg ctttgggtta atggtgagtt aatcggtggt
    22681 agtgatatta tcctacaaat gtaccaatca ggtgagctaa aaccactcat tgaagagcat
    22741 agcccaaagg tctgattact taatatctta aaataaatat caaaaaagac tgctgatgac
    22801 attagcggtc tttttttgat ggtcaagatt gatagtttgg attgaattat ttagccatat
    22861 tacatacaat aatcagcatg ctttgtatga attatcaatc aaactcaggc gaaggagtgc
    22921 ttgtggctaa aacatattta ttgactgcat tgataatgtc tatggtaatc tccggatgtc
    22981 aagtcatcca tgccaatcaa ggtaaggtta atactaattc tgctgtcatc gcaggtgcag
    23041 acgctcacac gcctgaacat gtaacgggac tgaccgaaca aaagcaggtg attgcaagtg
    23101 attttatagt agcgtcagcc aatccattag caacacaagc tggctatgat atcttaaagc
    23161 aaggcggtag cgctgcagat gcgatggtgg cggtgcagac gacactaagc ttggtagagc
    23221 cacagtcgtc aggcttgggc ggtggtgcat ttgtgttgta ttgggacaat accgccaaaa
    23281 cattgaccac atttgatggg cgtgagacgg caccgatgcg tgcgacgcca gaattatttt
    23341 tggataaaga tggttaacca ttgaaattta tggaagcggt ggtcgctcgg taggtacgcc
    23401 tgctatccct aaactgacgg agacaataca tcagcgatac ggtgtattgc cttggggaaa
    23461 attatttgat acgccgatcc acttggcaaa acaaggcttt gaggtgtcgc caaggcttgc
    23521 catctcggtt gagcaaaatc agcagcattt ggcacgctat ccaaaaacag ccgcttattt
    23581 tttgccgaat ggtgtgccgc tacaagcagg cagcttgctg aaaaatttag aatttgctga
    23641 cagtgttcag gcgttagcag ctcaaggtgc aaaagctctg catactggta aatatgccca
    23701 aaatattgtt tcagttgtcc aaaatgctaa ggataacccc ggtcaattat ccttgcaaaa
    23761 tttatctgat tatcaagtgg tggaacgccc gcctgtttgt gtgacttatc gtatttatga
    23821 agtatgcggt atgggtgcac caagctcagg tgggattgct gtgggtcaga ttttggggat
    23881 tttaaatgaa ttttcaccca atcgggtggg gtatgatgct gaaggtttgc gtctcttggg
    23941 cgatgcttct aggcttgcgt ttgcggatcg tgatgtatat ttgggcgacc ctgattttgt
    24001 accagtaccc attcgccagt tgatttctaa agactaccta aaacatcgca gccagctgct
    24061 tgagcagtcg gataaggcat tgcctagcgt atctgcaggc gattttattc atgagtgggc
    24121 gtcctcacaa gcgattgagt taccttctac cagtcatata tcaattgtgg ataaggcggg
    24181 caatgtgcta tcaatgacga cttccattga aaatgcgttt ggctcaactt tgatggcaaa
    24241 tggctacctg ctgaataatg agctgacaga ctttagtttt gagccaataa agcagggcaa
    24301 acaggtcgcc aatcgtgtcg agcctggcaa gagaccacgc tcctcaatga caccaaccat
    24361 cgtatttaaa gcaggcaagc cttatatggc aatcggctct ccaggtggta gccgtatcat
    24421 tggctatgtc gctaagacga ttgtggcaca tagtgactgg aatatggata tccaagatgc
    24481 catcagtgca cccaatttat taaatcgctt tggtagctat gaactagaaa cgggtacaac
    24541 cgccgtccag tggcaacaaa cattaaatga tttgggctat aagactgatg tgcgtgagct
    24601 aaattcaggt gttcaagcca ttattatcga gccaagcagg ctggttggtg gtgtcgatcc
    24661 acgccgagaa ggtcgagtga cgggtgatta gaggtgaagc ttgtattttg tgaatttgac
    24721 cgcattaagt taattattaa aataggggga aattgtgagt aatcaaacaa aaatacaagc
    24781 catcaagcaa gcttctgagc agatcttgac aatttgtgag acgccaaaca cagcattaca
    24841 agccattcat ctgattttaa gacatggcgg ggcaggaaga attgtcttgg caagtggtgt
    24901 atcagcgggt tatggctgat aaagatgtgg ttggtgctgg ttatttgatt gattttgcac
    24961 aaactgccga aaatttacca tttgatgtat tgcctttgat tagccttgtg ctaaacaaag
    25021 gtgatgagac gctaaaaacc ggcatgttaa ataaattacc tgataatgcc aaagaaaatt
    25081 tacgcattat gggctatctg ccttaggttt tgaagtattt ttttatacga aaaacgctca
    25141 tgataccctg attacacaaa ggagccgaga atatgttttc aaaaagcgtt accctcgcac
    25201 aatatgaccc cgatttggca gcagccattg cccaagaaga ccgacgccag caagaccacg
    25261 tcgagctgat tgcctctgaa aactacgtca gctgcgccgt gatggaggca caaggttcgc
    25321 aattgaccaa caaatacgcc gaaggctatc ccgcaaaacg ctactacggc ggctgcgaat
    25381 acgtcgatat tgtcgaacaa ttggcaatcg accgcgtaaa agaactgttt ggcgcagcct
    25441 atgccaacgt tcaaccgcac tccggttccc aagccaacca agccgtatat gcttccgttt
    25501 taaaaccggg cgacaccatt ttgggtatgt ctctggcgca cggcggacac ctgacccacg
    25561 gcgcgagcgt taatatttcc ggcaaactct acaatgccgt tacttatggc ttggatgaaa
    25621 acgaagtcct cgattatgcc gaagtcgaac gcctcgcgct cgaacacaaa cccaaaatga
    25681 ttgtggcggg cgcgtctgcc tacgcgttgc aaatcgactg ggcaaaattc cgcgaaatcg
    25741 ccgataaagt cggcgcatac ctctttgtcg acatggcgca ctatgccgga ctggttgccg
    25801 gcggcgaata tcctaacccc gtgccattct gcgacttcgt aaccactacc acccacaaaa
    25861 ccctacgcgg ccctcgcggc ggtgtgattt tgtgccgtga caatacccac gaaaaagcgc
    25921 tgaactcttc catcttccca agcctgcaag gcggtccgtt gatgcacgtt atcgccgcca
    25981 aagccgtagc gtttaaagaa gcgttgcaac ccgagttcaa acaatacgca aaacaagtga
    26041 aaatcaatgc tgccgtcatg gcggaagagt tggttaaacg cggtttgcgc atcgtttccg
    26101 gccgcaccga aagccacgtt ttcctcgtcg acctgcaacc gatgaaaatc accggcaaag
    26161 ccgccgaagc cgctttgggc aaagcgcaca tcaccgtcaa caaaaacgcc atcccgaacg
    26221 atccggaaaa accgttcgtt acctccggca tccgcatcgg ttccgccgcc atgaccactc
    26281 gcggctttaa cgaaaccgac gctcgcgtat tgtcgaactt ggttgccgat gtattggcta
    26341 atcccgaaga cgaagccaac ctcgccaaag tccgcggaca agtgactgcc ttgtgcgaca
    26401 aataccccgt ttacggaaac taaacgcccg atgtttccat acaaaatgcc gtctgaacat
    26461 tttccagacg gcattttttg ttttcggtaa cattgctatt ttttctcttc cagcaaaatg
    26521 accaactttt tcaatactgc gatttcacta tctttctgcc tgatcaattc atcctttgca
    26581 tccagcagcg acctcaattt ttcattttca aacaccagca tttcatcaga gccgataata
    26641 ttatgatggt tattggtatt gtcgccgata gagaagaaaa acgatcggtt ctgctccgcc
    26701 agcagctcaa ccacatcgat attgaaaatc tgggcaatct ggcgcagctt gtcgagattg
    26761 atgccgcttt tcccgcgttc tattttcgaa tacccgttaa ccgacatttc caatttttcc
    26821 gccatctctt cctgcgtcca ctgattgact tcccttaacg tccgaatctt gtcatgcact
    26881 tccatcctgc cctccaaggt taattttcac cactcaaaca accgtaaccg gtatcttcag
    26941 ttgatggtcg gatattttaa aaataaataa tataacacaa tagtattttg ataaaaattt
    27001 acctaacgag gtacagacat gtattcagaa aacaagccta aacgcaccgt aaaaatgaaa
    27061 aaatactggc agcctgcacc ctttccctgt ccctttctgc ctgtacttcg actcaagtaa
    27121 cttcttttgt cgagggactc tccgagggac tcgtagatgt cagcgaagcc tttatcacaa
    27181 caaaggcaga atcatattct gccccgtctc ttcaggataa gcaaattttc ataaatcgcg
    27241 tctcagaacg agcacaaaaa aattacaaag aaagaaggaa aaagaaagaa gcagacttac
    27301 taaattcagc atcgtcatta aatgaatcaa catcagaagc cctatctgcc cttaaaaacg
    27361 ctatcgtaat tcctaaacgc cctaaacgct ccttgggcaa aaaatataaa aacggttttc
    27421 atcctgtaaa tacgtgtgcg gatgtggaag ctcggtgggc aaacggagca atgagagatg
    27481 ttagggacat agaaaggcta ataagccgaa acgtcccttt acgtcgtatc ggagacgcca
    27541 ttcaaaacat gtgcatggaa accaatgtgt ccattaggat gtatcgccag gagtgccgtt
    27601 cagaatcgtc agatttttat gacgaaatgc ttgagcatca cgagaatttc ataggatcct
    27661 ccaaatcatt cgcatcatca cttcttcccc ttagcgttac aaactttaca acagacatgg
    27721 cgtgtactcc cgaattttaa tctaatcaca ctccacaact ccctatcaat ataactgcta
    27781 cttctctgag tagtatcaac caacccttaa caaaggacta aaaaatgaaa aaagcactgc
    27841 ttgcactgac tattgccgcc atctccggta ctgctatggc ccagttgccc gactttctgg
    27901 gtaaaggcga atataccgtc cgtacagaca tctccaaaca aacgctgaaa aatgccgatt
    27961 tgaaagaaaa acacaaagta caaaaaaaca tcggtttccg tgccgatatg ccgtttgacg
    28021 atattcacca cggcatgcgt ttcgaagtat cacacagccg agacaaaaaa gacatgtacg
    28081 ttgtgaccga aagtactact aaaccattcg gcaaagacgt taaagaaaaa cgcaccgacg
    28141 tgtatgccgg ttacacctac acccaaccgc tcagtgaagc caccaaactg cgtgccggtt
    28201 taggtttggg ctatgaaaaa tacaaagatg cggtagctaa tgagaaagga acagtcagca
    28261 ccgagcgcga agccttctat accaaagcgc acgccgacct gacttccgat ttgggcggcg
    28321 gctggtatct gaatccttgg gcagaggtta aggttgacct ggacgccaaa ttgaaacaca
    28381 acgcaactgt agccggtgcc tcagccgaca ttaacgcaaa aacacgcgga tggggcgtgg
    28441 gcgtaggtgc gaatatcggc aaacaaatta ccgataccgt cggcatcgaa gccggcccgt
    28501 tctacaaaca ccgtcacttc aaagcatccg gcagcttcgt tttagacggc gggaacatcc
    28561 gagtcgatcc taccaaaatc aacgaatacg gtgttcgtgt cggcgtgaaa ttctaatcgc
    28621 ggcgatatgc cccagacaca aaaccgcaag ccttttggtt tgcggttttt gatgttgtag
    28681 cagaccctaa taccgtctga aacttttcag acggcatttt tccaatcggt taaaataccg
    28741 cctcgacatt gtgttttgga aaacccgtgc ttgcttctgt gattgatttt atcctgcata
    28801 tcgaccaaca cctgctcagc ctgtcggcgc aatacggtgt gtggatttat gcgattctgt
    28861 ttttgattgt tttttgcgaa accggtctga ttgttacgcc cttcctgccc ggcgattcgc
    28921 tcttgtttgc cgccggcggg attgccgcgc tgggcggtat ggatattcat ttgatggttg
    28981 cattattaag ccttgccgcc atactcggcg acgcgctcaa ttttaccgtc ggcaaatatt
    29041 tcggcggcag gctgttcgcc aaccccgatt ctaaaatctt ccggtgcgaa tacctcggca
    29101 aaacccgccg tttttacgaa aaacacggcg gcaaaacgat tatcatcgcg cgcttcgtgc
    29161 ccatcgtccg caccttcgcc cccttcgtcg ccggtatggg aaaaatgcac tatgccaaat
    29221 ttatccgcta caacatcatc ggcggcttgt tgtgggttat cttgttttct tacgcgggct
    29281 atttctttgc caacttcccc gtcgtaaaaa acaatctggg cttggtgatg ggcggcatca
    29341 ttattgtttc cgtcctgccc ggcatcatcg aaatcgcccg cgccaaactt gcggcaaaat
    29401 ccgaacgtta attgttggaa attgacatta tgaatatatt atccgtagaa aacgcttcct
    29461 ttgccgtcgg acatgtcgcc ctgctcgaca aaacgtcctt ccaactcgac agcggcgaaa
    29521 aagtcggctt gatcggacgc aacggcgcag gcaagtcttc gtttttaaaa atcctcaccg
    29581 gcgtgcaaaa actcgacgac gggcagatta ttgttcaaaa caatctcaaa atcgtttatg
    29641 tgccgcagga gtcctttttt gataaggacg caaccgtatt tgatactgtt gccgaaggtt
    29701 tgggcgaaat ttgcgattta ttgcgccgtt atcatcatgt cagccatgag ttggaaaatg
    29761 gttcgagtga gctcttattg aaagagctta acgaattgca acttgaaatc gaagcgaagg
    29821 acggttggaa actggatgcg gcagtcaagc agactttggg ggaactcggt ttgccggaaa
    29881 acgaaaaaat cggcaacctt tccggcggtc agaaaaaacg cgtcgcctta gcgcaggctt
    29941 gggtacagaa gcccgatgta ttgttactgg acgaaccgac caaccatttg gatatcgacg
    30001 cgattatttg gttggaaaac ctgctcaaag cgtttgaagg cagcttggtc gtgattaccc
    30061 acgaccgccg ttttttggac aatatcgcca cgcgcattgt cgaactcgat cgcggtattt
    30121 tgcgctccta tcccggctcg ttctccaaat acagcgagaa aaaagcgcaa gagttggcgg
    30181 ttgaagccga acacaaccgc ctcttcgaca aattccacgc gcaggaagag gcgtggatac
    30241 gcaaaggcat cgaagcgcgc cgtacccgca acgaaggccg cgtgcgccgt ttggaagaac
    30301 tgcgccgcca gcgtgccgaa cgccgcaatg tacaaggaca ggtcaacttc aaactcgaca
    30361 gcggcaagaa aagcggcaaa atcatcgccg aactggaaca cgcttcgttt gcctatgacg
    30421 acaaagttat catggataaa ttttccgcca tcttgcagcg cggcgacaaa atcggcttaa
    30481 tcggcccaaa cggtatcggc aaaaccacct tccttaagct gattttgggc gaattgcagc
    30541 cgacctacgg cagaatccgt atcggcagca agcaggaagt cgcctatttc gaccagttcc
    30601 gcagcgcctt gaatgaaaac gatactgtgt tttacacgct cggacagggc aacgattacg
    30661 tcgaagtcgg cggcaagaaa aagcatgtga tgagctattt ggaagatttc ctgttccccc
    30721 ctgcccgcgc gcaaagtcct gtttcttcac tctccggcgg cgaacgcaac cgccttttgc
    30781 tggcaaaact ctttactcgt cccgccaata ttttggtttt ggacgaaccg accaacgact
    30841 tggacatcga cacccaagaa ttgcttgaag atctgttgcg cgattaccaa ggcacggtct
    30901 tcctcgtctc gcacgaccgt atgttcctcg acaatgtaat tacccaaagc attgttttcg
    30961 aaggacaagg ccgtctgaaa gaatacatcg gcggctatca ggactatatc gacgcaaaat
    31021 cgcgggaaaa taaaattcag acggcatccg cacccaaagc atccgatgtc gaacccgcca
    31081 aagaaaaacc caaagccaac cgtacggtca aactttccta caaagaacag cgcgaactcg
    31141 acgccctgcc cgacgaaatc gccgccttgg aaaccgaaca ggcagaaatc aacgcgcagc
    31201 tttccgatcc cggaattttc aaagattacg aaaaagcagg agcattgcaa aaccgcgccg
    31261 aagaaatcga aatgctgctt ttggaaaagc tggaacgctg ggagctgctg gaaaccaaac
    31321 agaacggcaa tgccgtctga aatcaaacag gaagaaaaaa aatgttcaaa cgacctgaag
    31381 aaatcatcgt cctgatactt gccgtcctct ggattgccgg tacgtatttt ctcgccgccc
    31441 tgtttggagc ggatgcttat accgtgttaa aaatcactgc ccttaccctg ctttggtctg
    31501 ccgcatcatt cctcctttgg caaaaaaaac ctcagccggc atatctggcc gctgctgctc
    31561 ggctgcctga ctacctgttg gtggccgtat ctgaatcaat cggtcgcacc cggttcttta
    31621 ccttggcatg catcatggac gttcaaaacc atcttagccc tgattcccgc aaccgcaggt
    31681 tatctgttta aatggaaaca ataccggcag cgcaaaacaa agccgtaagc ccgtcttttt
    31741 tcagacggca tctccggtca gactgtcaac aattccgata aaatgttata ctcgcgcccg
    31801 ttgccgcgcc gtccgaaacc gccttttggg aaagcagaaa tgatcagcaa acaagaatac
    31861 caagcccaag ccgcccaagg ctacaaccgc atcccgctcg tgcaagaact ccttgccgac
    31921 ttggatacgc cgctttccct ctatctcaaa ctcgccaacc gcccctatac ctacctgctc
    31981 gaatccgttg tcggcggcga acgtttcggc cgctattcct ttatcggcct gccttgcagc
    32041 cactatctca aagccggcgg caaacacgtc gatgtttatc aaaacggcga aatcgtcgaa
    32101 caatacgacg gcaatccgct accctttatc gaagctttcc acaaccgttt caaaacaccc
    32161 gaaatcccaa gcctgccgcg ctttaccggc ggcttggtcg gctacttcgg ttacgaaacc
    32221 gtctacaatt tcgaacactt cgcccaccgc ctgaaaaaca ccgccaaagc caacccgctc
    32281 ggcacgcccg atattttgct gatgctgtcg caagagttgg cggtaatcga caatttgagc
    32341 ggcaaaatcc acctcatcgt ttatgctgat ccctcgcagc ccgacagcta cgaacgcgcc
    32401 cgcgaacgcc tcgaagacat ccgcacccag ttgcgccaaa gctgcgccat cccactttcc
    32461 ctcggcagca aacaaaccca agcagtcagc gagttcggcg aagaaccttt caaagcctgc
    32521 gtcgataaaa tcaaagacta cattttcgca ggcgactgta tgcaggtcgt ccccagccaa
    32581 cgcatgagca tggaatttac cgacaatccg cttgccctct accgcgccct gcgcacgctc
    32641 aacccttcgc cttacctctt ttactacgat ttcggcgatt tccacatcgt cggttcctcg
    32701 cccgaaatcc tcgtccgccg cgaacgcgac gacgtcatcg tccgccccat cgccggcacg
    32761 cgcctgcgcg gcaaaacccc cgccgaagac cttgccaacg aacaagattt gttaagcgac
    32821 gccaaagaaa tcgccgaaca cgttatgctt atcgatttgg ggcgcaacga tgtcggacgc
    32881 atcagcaaaa caggcgaagt caaagtcacc gacaaaatgg tgattgaaaa atactcccat
    32941 gtgatgcaca tcgtttccaa cgtcgaaggc tgtctgaaag agggcgttac caatatggac
    33001 atcctcgccg ccaccttccc tgccggcaca ctctccggcg cgccaaaagt ccgcgcgatg
    33061 gaaatcatcg aagaaatcga accggagaaa cgcggcatct acggcggcgc cgtcggcgta
    33121 tggggcttca acaacgatat ggatttggca atcgccatcc gcaccgccgt gattaaaaac
    33181 aataccctct tcatccaaag cggcgcgggc gtggttgccg actccgaccc aacttccgaa
    33241 tggcaggaaa ctcaaaacaa agcccgcgca gtggttcggg cggcgcagat ggtgcaggaa
    33301 gggttggata aataaatttc agacaacctt cgcagatgaa aagaaggtcg tctgaaaatc
    33361 acaaaacaac atcagagata cttacataaa aaatcacaat gcaattcaca tcattagaaa
    33421 tttgtgccgg agcaggcgga caggctttgg gcttagaaag ggcaggcttt tcccatgttg
    33481 cgttaatcga aatcgaaccg tcggcttgtc aaaccctacg tttaaaccgt cctgattgga
    33541 acgttattga aggagatgtc cgcttgtttc agggcgaagg atatgacggt attgatttat
    33601 tggccggcgg cgtgccttgc cctccttttt ccaaagctgg aaaacaactg ggaaaagatg
    33661 atgaacgcga tttattccct gaggctattc gtctagctaa ggaaactgac cctaaagcca
    33721 tcatgttaga aaatgtgcgg gggttacttg acccaaaatt tgaaaattat cgcaaccata
    33781 ttacagaaca atttgccaag ttgggttacc ttggacaatg gaaactactt tatgctgccg
    33841 attacggagt atcgcaactc agacccaggg ttttatttgt tgcgttgaaa aacgaataca
    33901 ccaatttttt caaatggcct gaaccaaatt cggaacaacc gaaaaccgtt ggagaactgc
    33961 tttttgactt aatgtcagaa aataactggc agggggcgca taactggcgt ttgaaggccg
    34021 cgcagattgc accgaccttg gtcggcggtt caaaaaaaca cggcggtgct gacttggggc
    34081 ctacacgatc caaacgcgca tgggcggagt tgggtgtgga tggttcaggt ttgtgggata
    34141 gtgcgccgcc tgaagacttt tcgggaatgc ctagattgac tgtccgaatg acggcgcgta
    34201 tccaaggatt tcctgataac tggcaatttt ttggtaaaaa aacgcctatg taccgccaaa
    34261 tcggcaacgc ttttcccccg ccggtagccg aagcggtggg caggcagatt atcaaagcct
    34321 taaaaaagga aaattgaatg aatccgcttt ttacccaaga acgcaggatt ttccataaga
    34381 aattattgga tggcaatatt cttgcaacca acaatagagg cgtagtcagc aatgcggatg
    34441 ggagcaatac ccgaagtttc aatatagcca aggggattgc cgacttactg cattcggaaa
    34501 cggtttcgga aagattaccc ggtcagacat cgggaaatgc ttttgaagca atatgcagtg
    34561 agtttgtcca atctgctttt gagaaattgc agcatatcag acccggcgac tggaatgtta
    34621 agcaggtcgg ttctcgcaac cgtctggaaa tcgcacgtta tcagcaatac gcacatctga
    34681 cagccttagc caaggctgcc gaagaaaacc ccgaattggc tgccgccttg ggcagtgatt
    34741 acaccattac tcccgatatt atcgttacca gaaatttgat tgcagatgca gaaatcaacc
    34801 gtaatgaatt tttagttgat gaaaatattg ccacatatgc cagtttgcgt gcgggtaatg
    34861 gcaatatgcc gctgctccac gccagtatct cttgtaaatg gacgattcgc agcgacaggg
    34921 ctcaaaatgc ccgttctgaa ggattgaatt tggttagaaa cagaaaaggc agactgccgc
    34981 acattgttgt cgtaacggca gaaccaacgc caagccgcat ttcatctatt gccttgggta
    35041 caggggaaat agattgcgtt taccattttg ccttatatga gctggaacaa attttacaat
    35101 cattgaatta tgaagacgct ttggatttgt tttacatcat ggtcaatggt aaacgattaa
    35161 aagatatttc cgaccttccc ttagatttgg cagtttaaac agaattattc cactatgaac
    35221 acacccccca tccttcctcc cgccatgctc ggcatcctcg gcggcggaca attaggcaga
    35281 atgtttgccg ttgccgctaa aaccatgggc tacaaagtaa ccgttctcga tcccgacccg
    35341 aatgcgccgg cggcggaatt tgccgaccgc catttgtgcg cgccgtttga cgaccgggcc
    35401 gcgttggacg aattggcaaa atgcgcggcg gttacgaccg aatttgaaaa cgtcaatgcc
    35461 gacgcgatgc gctctctggc aaagcatacc aacgtttccc ccagcggcga ctgcgtgtcc
    35521 attgcacaaa accgcattca ggaaaaagcg cggatacgca aagcaggctt gcaaaccgcg
    35581 ccgtatcagg cggtttgcaa ggccgaagac attactgaag caagcgcgca atttttgccc
    35641 ggcatcctga aaacggctac gttgggctac gacggcaaag gtcaaatccg cgtcaaaacg
    35701 ttggacgaac tcaaagccgc gtttgccgaa cacggcggcg tggattgcgt tttggaaaaa
    35761 atggtggact tgcgcggcga gatttccgtg atcgtatgcc gtctgaacga tgaaaacgtg
    35821 caaaccttcg accccgccga aaacatccac gaaaacggca tcttggctta ttccatcgtc
    35881 cccgcgcggc tgagtgccga cgtgcagcaa caggcgcggc agacggcgca acgcttggcg
    35941 gacgaattgg attatgtcgg cgtattggcg gtagaaatgt ttgttgtcgg cgacacacat
    36001 gaattgctcg tcaatgaaac cgccccgcgc acgcacaatt ccggccacca tacgatagat
    36061 gcctgcgccg cagaccagtt ccaacagcag gtacgcatta tgtgcaacct gccgcccgcc
    36121 gacaccaaat tattatcccc ttgctgtatg gcgaatattt tgggcgacgt ttggcaggaa
    36181 gatggcggcg aaccggattg gctgccgttg caaagccggc cgaatgcaca cctgcaccta
    36241 tacggaaaaa aaaccgcaca gaaaggtcgg aaaatgggac actttaccgt tttgaccacc
    36301 gattcggaca ccgcatttca agaagcaaaa aaactgcatc agtccctata atccgaccaa
    36361 cggcacagat gccgtctgaa gccgccgaca taccgccatg agcctgctga cactgttacg
    36421 ccccgccacc gtgcaagact gcaaagacat cttcaaagtc cacctgcact ccgtccaata
    36481 cacctgcatc ctcagctaca acgaacacgc cctaaaagta tgggaaggct tgttaaacac
    36541 agaaagctac ctgccgacca tttccgatcc cgacaaagca ctttgggttg ccgaatacaa
    36601 aggcaacata cagggatttt tccaaatcga ctgccaagaa gcacaactcg atgcgcttta
    36661 cgtccacccc ctgttccaca atctcggact gggcactgcc ctgctccgtc aggcagaaac
    36721 catcgcccac aaatccggtt tgagctttct gaaactctac gcatctctca actccgtccc
    36781 gttttacctt ctcaaccgct acgaatcgct cggttcggca gtattgcagc ttgaccccag
    36841 catcaaaatc aaatgcgaac tgatgcgcaa acacctctaa tcccgcctta taaccaaaac
    36901 tgataatctt caaatcaaac attatttcca caagcgcaga cagccgtata aagttgcccc
    36961 atctaccaac cgacaggaca agcatatgaa aacctacgca caggcactct ataccgcagc
    37021 cctgctcacc gcctgcagcc ccgcagccga ttcaaaccat ccgtccggac aaaatgcccc
    37081 ggccaatacc gaatccgacg gaaaaaacat taccctgctc aatgcctcat acgatgtgac
    37141 acggtatttt tacaaagaat acaaccactt atttatcaaa acataccaat ccgaacaccc
    37201 cggcacatcc gtcagcatcc aacaatccca cggcggcttc agcaaacagg cattatccgt
    37261 agccaacggc cttcaagccg atgtcgtaac catgaaccaa tcttccgaca tcgacctgct
    37321 cgaaaaaaag gactggtaga aaaaggctgg caacaagccc tccccgatca cgccgcaccc
    37381 tacaccagca ctatggtttt ccttgtccga aaaaacaacc ccaaacagat ccgcgattgg
    37441 aacgaccttg ccaaagacgg cgctaacatc gtcatcgcca agacctcggg caacggacgc
    37501 tacgccttcc tcggcgcata cggttacggt ctgaaagcca acaacggcaa cgagcaggaa
    37561 gcccaaaaac tcgtcgcatc catcctcaaa aacacacccg tttttgaaaa cggcggacgc
    37621 gccgccgcca ccaccttcac acaacgcaac atcggcgacg tactcatcac ttttgaaaac
    37681 gaagccaact acgtcagcaa aaaactgacc caaggacagt ttgaaatcgt ctatccgagc
    37741 tacaccattt ccgccgaaag ccccgttgcc gtcgtcaaca gcgtcgtcgc gaaaaaaggc
    37801 acgcaaaaaa ccgcccgcgc ctatctcgaa tacctttgga gcgaaccggc gcaagaactc
    37861 gccgcctcac tctacctgcg tccccgcaac cccgaagtat tggcacgaca caaagccgac
    37921 ttccccgact tagacacctt ccctcccgaa gaaaaattcg gcggatggga caacatcatg
    37981 aaaacctact tcgccgacgg cggcgtattc gaccgcctga ccgcacaaaa atgaccatta
    38041 tgccgtctga accgcctttc agacggcatt tcacacatca aaacagaaaa ccgcacccaa
    38101 aatcatcatc acaagacaca acggcaaacc ggatacaaaa cccaacaccg ccgccaaaat
    38161 gccgtctgaa gccgtcggcg caaccatcct tatctggggc gcgcctcatc aaaggggcgc
    38221 ataactttcc ttaaccatcc cttttgacag ccaaataaac agtgcttttg cctgtcagaa
    38281 acaaaaccaa tattttgcgg ttcgtatcgg aacatttata aaacatcaga tttccgtgtt
    38341 tcgcgtttcc ggcaattcaa gattagcaaa accattaacc tgcttcgcaa ggcaggtttt
    38401 taaaacttta tcaggcgttc gagcatatcg gttcgaccga cccgcatttc cggcaatcaa
    38461 cactgccgac ggttgccgca tttctttttg atatacaatc acactttcaa aaaacatacg
    38521 acggcaacaa aatcatcctc cccccgcttg gcaaacatcc ggcaaattaa aaccccgccc
    38581 gaaacagcca tcggacaaat aacaggaaaa aatatgaata ccgacttcca aaagatcctc
    38641 aaacaactgg gcaaacaggc caaaaaagaa gcggcagaaa aacaggaagc cgaaaaaaac
    38701 aagcaaaaac aagaatatga tttggatttt tcacaagcag tcggacaagt ctccccatta
    38761 aaaaaccggc agcaatatta tgcgcagtcc gacaaaaccc ccatcaaagc ccgtcccaaa
    38821 gacaaccgca ccgacgaaga aaactacttc tacataggca gcacatacaa cgacccaccc
    38881 gccagcttct gcaaaaacgg acagggaaaa aacgacatcc aacgcctgaa aaacggatac
    38941 tatcccgtcg ttaccgatgt cgacctgcac ggctacaccc aagaagaagc acaaaaagtc
    39001 ctcaacgaat tcatcgcatt cacccaaaaa cgcggcgtat gcggcgaaat catccacggc
    39061 agcggattag gttccaaagg ctacaaaccc gttctgaaaa acatgacccg aaactggctg
    39121 atgcaacacc ccgacgtact cgcctacgtc gaaccccggg aaggcaacga cggctgcgtc
    39181 cgcatcctgc tcaaacgcaa attaaggcag caagactgac aaatttctcc gacagacttg
    39241 gcagcaccgc acaatatccg tataatttgc aaaccatttc tgggggcgac cttggtttcg
    39301 acgggggttg cgaagcagat gcgggcatac cggggtctca gattcccgta aaacactgaa
    39361 ttcaaatagt cgcaaacgac gaaacttacg ctttagccgc ttaaggctag ccgttgcagc
    39421 agtcggtcaa tgggctgtgt ggcgaaagcc accgcaacgt catcttacat tgactggttt
    39481 ccagccgggt tacttggcag gaaataagac ttaaggtaac tggtttccaa aaggcctgtt
    39541 ggtcggcatg atggaaataa gattttcaaa tagacacaac taagtatgta gaacgctttg
    39601 tagaggactt tcggacgggg gttcgattcc ccccgcctcc accatgccct attttcagca
    39661 gtatcctgca acacaaaaaa tccttgaact tcaaaagttt caaggctttt ttattgcctt
    39721 gaatatgatg gcgtatgctt gagttttcgc aatttatggg gtaacttttg gggtaacttt
    39781 tcaggaatca ccctaaaaaa tatcttaaaa atacaaaagc catcacgcat tttttaaaaa
    39841 ggttacccaa aacttaaatg aagcctttat ttataaggct tgcagctttc caaacaagtt
    39901 acccccaaac ggataaaaaa tgccgaag
//
LOCUS       DAWTCI010000007        39695 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWTCI010000007 DAWTCI010000000
VERSION     DAWTCI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39695
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.007"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            42..2219
                     /locus_tag="WM481_000419"
     CDS             42..2219
                     /locus_tag="WM481_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFA9079814.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPFGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     PDGTGEPSATVNLVRKHPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQLERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWSNSRNRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHSTAATDLIPGYWHADPRTHSASMSLTGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYDTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRAGSYNSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQLQ
                     KDEHGSYLKPVTGNNLEADIKGEWLEGRLNASAAVYRARKNNLATAAGRDQSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKPRDQDGSRPNPDSVPERSFKLFTAYH
                     LAPEAPSGRTIGAGVRRQGETHTDPAALRIPNPAAKARAVANSRQKAYAVADIMARYR
                     FNPRTELSLNVDNLFNKHYRTQPDRHSHGALRTVNAAFTYRFK"
     gene            complement(2482..2805)
                     /locus_tag="WM481_000420"
     CDS             complement(2482..2805)
                     /locus_tag="WM481_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFA9079815.1"
                     /translation="MDIVQLPSAALKAWMDEGRMFCLLDVRTDEEAAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLAEAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(2886..3542)
                     /locus_tag="WM481_000421"
     CDS             complement(2886..3542)
                     /locus_tag="WM481_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFA9079816.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAERQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVTLVPEVL
                     KEQLKDGGRMAVIVGRRPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDAHPFDSFNF
                     "
     gene            complement(3644..4147)
                     /locus_tag="WM481_000422"
     CDS             complement(3644..4147)
                     /locus_tag="WM481_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFA9079817.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            4331..5104
                     /locus_tag="WM481_000423"
     CDS             4331..5104
                     /locus_tag="WM481_000423"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFA9079818.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            5111..5461
                     /gene="secG"
                     /locus_tag="WM481_000424"
     CDS             5111..5461
                     /gene="secG"
                     /locus_tag="WM481_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFA9079819.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            5475..5560
                     /locus_tag="WM481_000425"
     tRNA            5475..5560
                     /locus_tag="WM481_000425"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5509..5511,aa:Leu,seq:gag)
     gene            6362..6730
                     /locus_tag="WM481_000426"
     CDS             6362..6730
                     /locus_tag="WM481_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFA9079820.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIEL"
     gene            6727..7038
                     /locus_tag="WM481_000427"
     CDS             6727..7038
                     /locus_tag="WM481_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFA9079821.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            7148..7358
                     /locus_tag="WM481_000428"
                     /pseudo
     CDS             7148..7358
                     /locus_tag="WM481_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7808..8363)
                     /locus_tag="WM481_000429"
                     /pseudo
     CDS             complement(7808..8363)
                     /locus_tag="WM481_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8360..9061)
                     /locus_tag="WM481_000430"
                     /pseudo
     CDS             complement(8360..9061)
                     /locus_tag="WM481_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950970.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(9298..10089)
                     /locus_tag="WM481_000431"
     CDS             complement(9298..10089)
                     /locus_tag="WM481_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFA9079822.1"
                     /translation="MNLFFDTQLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKDWLLAVMKCIDQLTEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNMIEFIGRGLYKKIDKLHPTPKAF"
     gene            complement(10318..12948)
                     /locus_tag="WM481_000432"
     CDS             complement(10318..12948)
                     /locus_tag="WM481_000432"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFA9079823.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            13215..13731
                     /locus_tag="WM481_000433"
                     /pseudo
     CDS             13215..13731
                     /locus_tag="WM481_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            13738..14181
                     /locus_tag="WM481_000434"
     CDS             13738..14181
                     /locus_tag="WM481_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFA9079824.1"
                     /translation="MPPRLLSGILCCLLTTAPVFAQGQPDAVSAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            14350..16407
                     /gene="ppk1"
                     /locus_tag="WM481_000435"
     CDS             14350..16407
                     /gene="ppk1"
                     /locus_tag="WM481_000435"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFA9079825.1"
                     /translation="MTEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRPDNGKTPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFRLTDAELYQVKGPVNLVRLNAVPDLVNRPDLKFPPHTPGRLKALGKNSPIFD
                     LVRQSPILLHHPYQSFDPVVDMIREAAADPAVLAVKMTIYRTGTRSELVPALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGLITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIGRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIVGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     ATPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWDL
                     LRG"
     gene            complement(16556..17659)
                     /locus_tag="WM481_000436"
     CDS             complement(16556..17659)
                     /locus_tag="WM481_000436"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFA9079826.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGRGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDNRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLGQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(17739..19295)
                     /gene="dnaA"
                     /locus_tag="WM481_000437"
     CDS             complement(17739..19295)
                     /gene="dnaA"
                     /locus_tag="WM481_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFA9079827.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRPAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEKPVKPAASKTAADILAERMKNLPHEPRQAAGPASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAENPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            19587..19721
                     /gene="rpmH"
                     /locus_tag="WM481_000438"
     CDS             19587..19721
                     /gene="rpmH"
                     /locus_tag="WM481_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFA9079828.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            19724..20089
                     /locus_tag="WM481_000439"
     CDS             19724..20089
                     /locus_tag="WM481_000439"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFA9079829.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            20154..20375
                     /gene="yidD"
                     /locus_tag="WM481_000440"
     CDS             20154..20375
                     /gene="yidD"
                     /locus_tag="WM481_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFA9079830.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGLRLAIKRIARCHPFGGHGHDPVP"
     gene            20548..22185
                     /gene="yidC"
                     /locus_tag="WM481_000441"
     CDS             20548..22185
                     /gene="yidC"
                     /locus_tag="WM481_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFA9079831.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQKQAATA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFVLFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYTLNGDTVEVRLSAPETNGLKIDKVYTFTKD
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCHIDI
                     KRRSDKLYSASVSVPLAAIQAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIVVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQT
                     IKEKYGDDRMAQQQAMMQLYKDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(22266..22976)
                     /locus_tag="WM481_000442"
     CDS             complement(22266..22976)
                     /locus_tag="WM481_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9079832.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLQHEQRAVVGLTDFVVEAEKTARA
                     HLKHLGITTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFNGYLPSEKNERIQSLNALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQLVVSKTVADWRRM
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(23038..23628)
                     /gene="maf"
                     /locus_tag="WM481_000443"
     CDS             complement(23038..23628)
                     /gene="maf"
                     /locus_tag="WM481_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HFA9079833.1"
                     /translation="MGLELPLVLGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPGALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMHRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCAAKSEGL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            23776..24276
                     /locus_tag="WM481_000444"
     CDS             23776..24276
                     /locus_tag="WM481_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFA9079834.1"
                     /translation="MSDPNLIDPEIFAAERQNLQGSFLLEELDERVSLHDYPADRRTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSDEESLDES
                     MLADEELEGILIEKELDVRALVEDQILMSLPFSPRHGHCGNTLPESANQDKPNPFAVL
                     AGLKSS"
     gene            24310..24489
                     /gene="rpmF"
                     /locus_tag="WM481_000445"
     CDS             24310..24489
                     /gene="rpmF"
                     /locus_tag="WM481_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFA9079835.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALFVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            24664..25191
                     /locus_tag="WM481_000446"
     CDS             24664..25191
                     /locus_tag="WM481_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFA9079836.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNAGVKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEVLRGKNVLVVDEVDDS
                     RVTMEFCLKELLKEDFDTVGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            25281..26327
                     /gene="plsX"
                     /locus_tag="WM481_000447"
     CDS             25281..26327
                     /gene="plsX"
                     /locus_tag="WM481_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFA9079837.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALNAAGAPMERIDICHTTQVVGMDESPQSALKNKKYSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDKTGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALEAAQNETAASL"
     gene            26465..26716
                     /locus_tag="WM481_000448"
     CDS             26465..26716
                     /locus_tag="WM481_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFA9079838.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFDILCTYIEDFDIRLTEQESREISFINKEFEIESTSD"
     gene            26743..26961
                     /locus_tag="WM481_000449"
                     /pseudo
     CDS             26743..26961
                     /locus_tag="WM481_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27115..27381
                     /locus_tag="WM481_000450"
     CDS             27115..27381
                     /locus_tag="WM481_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079839.1"
                     /translation="MPIIQTILEGGDIKVKNLKTENYSGKAREILGDSYPIEHISKNI
                     STIQNIISEFIRQKQTPAPNNLMETQQSRCRLKQLGLSDGISFT"
     gene            27333..27536
                     /locus_tag="WM481_000451"
     CDS             27333..27536
                     /locus_tag="WM481_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079840.1"
                     /translation="MPSEAAWAFRRHFLHLKQRIGNPAPPVHGNPHLKTICMPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            27563..28525
                     /locus_tag="WM481_000452"
     CDS             27563..28525
                     /locus_tag="WM481_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HFA9079841.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALADAKLNINDIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAEQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDAGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            28572..28943
                     /locus_tag="WM481_000453"
     CDS             28572..28943
                     /locus_tag="WM481_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFA9079842.1"
                     /translation="MKRQAFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWTGAYLGSRMFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            28969..29895
                     /gene="fabD"
                     /locus_tag="WM481_000454"
     CDS             28969..29895
                     /gene="fabD"
                     /locus_tag="WM481_000454"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFA9079843.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEHAIVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEVGGKTPAAVAGHSLGEYTALVAAEA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICAESAQGEVVEAVNFN
                     SPGQVVIAGNAAAVGRTMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDAGKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQITAFIEAH"
     gene            30034..31899
                     /gene="msbA"
                     /locus_tag="WM481_000455"
     CDS             30034..31899
                     /gene="msbA"
                     /locus_tag="WM481_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFA9079844.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAGIISTLQNWREQFTYMVWGTENKIWTVPL
                     FLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLMN
                     MLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRYY
                     RDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSKK
                     ITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKSL
                     ANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKAL
                     DNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLRA
                     QFALVSQDVFLFDDTLFENVRYSRPDAGESEVLSALQAANLQSLIDASPLGLHQPIGS
                     NGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGII
                     VAHRLTTVESADRIIVMDGGKIIEQGTHDQLMFQNGYYTMLRNISGKDTAAVQTA"
     gene            31999..33564
                     /gene="guaA"
                     /locus_tag="WM481_000456"
     CDS             31999..33564
                     /gene="guaA"
                     /locus_tag="WM481_000456"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFA9079845.1"
                     /translation="MTQDKILILDFGSQVTRLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSGLTRGIQDDAPNTLDVWMSHGDKVSKLPDGFAVIGDTPSCPIAMMEN
                     TEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYYYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            33626..34372
                     /gene="fabG"
                     /locus_tag="WM481_000457"
     CDS             33626..34372
                     /gene="fabG"
                     /locus_tag="WM481_000457"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFA9079846.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKIIGTATGESG
                     AAAISKRLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            34437..34922
                     /locus_tag="WM481_000458"
     CDS             34437..34922
                     /locus_tag="WM481_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFA9079847.1"
                     /translation="MNTHHHLPCPCGSGTSYAGCCRPLHLRQILPPTAEALMRSRYGA
                     YVLHLIDYIIATTVPAQQTFLDAAELMQWSREAEWLGLNVIAHRNLGKQHAQVEFEAY
                     FQDGGNRTVHHELSAFVKIAEQWYFIDPAVPLPTMKQPCICGSGKKFKACCGKYLQPV
                     A"
     gene            complement(35140..35934)
                     /locus_tag="WM481_000459"
     CDS             complement(35140..35934)
                     /locus_tag="WM481_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFA9079848.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSRRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            36038..36763
                     /locus_tag="WM481_000460"
     CDS             36038..36763
                     /locus_tag="WM481_000460"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFA9079849.1"
                     /translation="MPRFYLPENLSVGQTVDLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHTARLTVLEKHRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTRLIMSINRACKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFT"
     gene            complement(37031..37873)
                     /locus_tag="WM481_000461"
     CDS             complement(37031..37873)
                     /locus_tag="WM481_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9079850.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLERA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGVPYVAVFEDDVLFGKDAEKFLA
                     EDTWLEERFDKDSAFVVRLETMFAKVIVRPDKVLNYENRSFPLLESEHCGTAGYIISR
                     EAMRFFLDRFAVLPAERIKAVDLMMFTYFFDKEGMPVYQVSPALCTQELHYAKFLSQN
                     SMLGSDLEKDREQGRRHRRSLKAMFDLKRALGKFGREKKKRMESQRQAELEKVYGRRV
                     ILFK"
     gene            complement(37892..38905)
                     /locus_tag="WM481_000462"
     CDS             complement(37892..38905)
                     /locus_tag="WM481_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9079851.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLSAVVGQTWRNLDILIVDDGSTD
                     GTPAIARRFQEQDGRIRIISNPRNLGFIASLNIGLDELAKSGGGEYIARTDADDIASP
                     GWIEKIVGEMEKDRSIIAMGAWLEVLSEENNKSVLAAIARNGAIWDKPTRHEDIVAVF
                     PFGNPIHNNTMIMRRSVIDGGLRFDPAYIHAEDYKFWYEAGKLGRLAYYPEALVKYRF
                     HQDQTSSKYNLQQRRTAWKIKEEIRAGYWKAAGIAVGADCLNYGLLKSTAYALYEKAL
                     SGQDIGCLRLFLYEYFLSLEKYSLTDLLDFLTDRVMRKLFAAPQYRKILKKMLRPWKY
                     RSY"
     gene            complement(38965..>39695)
                     /locus_tag="WM481_000463"
     CDS             complement(38965..>39695)
                     /locus_tag="WM481_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFA9079852.1"
                     /translation="LEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLDTDVLVR
                     DGLKPLWDTDLGGNWVGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINLKKWRRH
                     DIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMANGFASRH
                     TDPLYLDRTNTAMPVAVSHYCGSAKPWHRDCTVWGAERFTELAGSLTTVPEEWRGKLA
                     VPPTKRMLQRWRKKLSARFLRKIY"
BASE COUNT         9842 a        11864 c         9525 g         8464 t
ORIGIN      
        1 ctatacaacc ccaccgcaca ccaacacaca aaggaaaccc catgacacgc ttcaaatact
       61 ccctgctttt tgccgccctg ctacccgtgt acgcgcaggc cgatgtttct gtttcagacg
      121 accccaaacc gcaggaaagc accgaattgc cgaccatcac cgttaccgcc gaccgcaccg
      181 cgagttccaa cgacggctac accgtttccg gcacgcacac cccgttcggg ctgcccatga
      241 ccctgcgcga aatcccgcag agcgtcagcg tcatcacatc gcaacaaatg cgcgaccaaa
      301 acatcaaaac gctcgaccgc gccctgttgc aggcgaccgg caccagccgc cagatttacg
      361 gctccgaccg cgcgggctac aactacctgt tcgcgcgcgg cagccgcatc gccaactacc
      421 aaatcaacgg catccccgtt gccgacgcgc tggccgatac gggcaatgcc aacaccgccg
      481 cctatgagcg cgtggaagtc gtgcgcggcg tggcggggct gccggacggc acgggcgagc
      541 cttctgccac cgtcaatctg gtacgcaaac acccgacccg caagccattg tttgaagtcc
      601 gcgccgaagc cggcaaccgc aaacatttcg ggctgggcgc ggacgtatcg ggcagcctga
      661 acgccgaagg cacgctgcgc ggccgcctgg tttccacctt cggacgcggc gactcgtggc
      721 ggcagctcga acgcagccgc gatgccgaac tctacggcat tttggaatac gacatcgcac
      781 cgcaaacccg cgtccacgca ggcatggact accagcaggc gaaagaaacc gcagacgcgc
      841 cgctcagcta cgccgtgtac gacagccaag gttatgccac cgccttcggc ccaaaagaca
      901 accccgccac aaattggtcg aacagccgca accgtgcgct caacctgttc gccggcatag
      961 aacaccgctt caaccaagac tggaaactca aagccgaata cgactacacc cgtagccgct
     1021 tccgccagcc ctacggtgtg gcaggcgtac tttccatcga ccacagcact gccgccaccg
     1081 acctgattcc cggttattgg cacgccgatc cgcgcaccca cagcgccagc atgtcattga
     1141 ccggcaaata ccgcctgttc ggccgcgagc acgatttaat cgcgggtatc aacggctaca
     1201 aatacgccag caacaaatac ggcgaacgca gcatcattcc caacgccatt cccaacgcct
     1261 acgaattttc ccgcacgggc gcctatccgc agccatcatc gtttgcccaa accatcccgc
     1321 aatacgacac caggcggcaa atcggcggct atctcgccac ccgtttccgc gccgccgaca
     1381 acctttcgct gatactcggc ggcagataca gccgctaccg cgcaggcagc tacaacagcc
     1441 gcacacaagg catgacctat gtgtccgcca accgtttcac cccctacaca ggcatcgtgt
     1501 tcgatctgac cggcaacctg tcgctttacg gctcgtacag cagcctgttc gtcccgcaat
     1561 tgcaaaaaga cgaacacggc agctacctga aacccgtaac cggcaacaat ctggaagccg
     1621 acatcaaagg cgaatggctt gaagggcgtc tgaacgcatc cgccgccgtg taccgcgccc
     1681 gtaaaaacaa cctcgccacc gcagcaggac gcgaccagag cggcaacacc tactaccgcg
     1741 ccgccaacca agccaaaacc cacggctggg aaatcgaagt cggcggccgc atcacgcccg
     1801 aatggcagat acaggcaggc tacagccaaa gcaaaccccg cgaccaagac ggcagccgcc
     1861 cgaaccccga cagcgtaccc gaacgcagct tcaaactctt caccgcctac cacttagccc
     1921 ccgaagcccc cagcggccgg accatcggtg cgggtgtgcg ccggcagggc gaaacccaca
     1981 ccgacccagc cgcgctccgc atccccaatc ccgccgccaa agcccgcgcc gtcgccaaca
     2041 gccgccagaa agcctacgcc gtcgccgaca tcatggcgcg ttaccgcttc aatccgcgca
     2101 ccgaactgtc gctgaacgtg gacaacctgt tcaacaaaca ctaccgcacc cagcccgacc
     2161 gccacagcca cggcgcactg cggacagtga acgcggcgtt tacctatcgg tttaaataag
     2221 gtcgtctgaa aacggagttt ctgcgaagct aaaagcagcc tgcacattga aaatgccgcc
     2281 caagcaaacc tgcagtttgc cgccctctcc ctagccttct cccacggggc gagggaattg
     2341 gggtgcaggc tgcctttaag gttcaggcaa attttaactt cgttgatact gcgctttagc
     2401 ttcgcagaag ctgcactttc agacgacctt tatggaacac cagaggtaca cgcatttaag
     2461 gaatgccgtc tgaagccgcc ttcaataccg cgccatttcc gcgtcaacct caaccgccca
     2521 cgcgtcgatg ccgccctgaa ggttgtacag gttttcaaaa cccgcctccg ccaaatacat
     2581 cgccgtatgc aggctgcgga taccgtgatg gcaatacacc acaagcggca catcgtccgg
     2641 caactcgttt tgccgcagcg gaatcagatt catcgggata tgcagcgcat ttggcagcga
     2701 acaaaccgcc gcttcttcat ccgtacgcac gtccaacaaa caaaacatcc gcccttcgtc
     2761 catccacgcc ttcaatgccg ccgacggaag ttgcacaata tccatcacaa accccccgaa
     2821 aaaaaccaag caaaatgccg tctgaagccc caaacccgct ttcagacggc atcacaatcc
     2881 gaacatcaaa aattaaaact gtcgaaagga tgggcatcct tgtcgtccaa atgcgccacc
     2941 aaagtatcga acagcacctt ctcttcaaac acatcgcccc tgcgcgtaat caaaagcgcg
     3001 cgctgcaccg gcctgcgtcc cacaataacc gccatacgcc cgccgtcctt caactgttcc
     3061 tttaacactt caggcacaag ggttaccgcg ccgccgacgt aaaccgcatc aaacggcgca
     3121 cctgcggaaa gttcggtcaa cccgttattt tgcacataat cgatgttttc caaagacaag
     3181 ccgtccaaaa ccgctttggc gcgcttttgc cgttcggcat cgatgtcgtc cgaaaccaca
     3241 cgaccagcca attttgccaa aagcgcggtc gcatagcccg aacccgtgcc gatttccaaa
     3301 accgtatcgt ttttcgtcag cttcaagccc tgcgccagcc gcgccacgac tttcggctcc
     3361 agcatcttat gaccgttggc aagcggcagc gccatatccg catacgccaa accctgcaaa
     3421 tcctcatcga caaaaagctc gcgcggaatc tccgccaaag cgtccaaaac gtcaaaatcc
     3481 aatacatccc acggacggat ttgctgttcg accatattga accgcgcttt ttcaaaatcc
     3541 atttagtgct ccgtcaaata attgtttcaa caatggcagg cattataaaa ccacactccc
     3601 gccgcgccaa cttcggcacg cgccgcccgc ccctttccat cactcaagcc gcctggcgcg
     3661 aagcctcaaa ccgcttctcc aaaatcttcg ccagttcgcc caaatacaaa gcatctgtct
     3721 catcaaactg cgccaaatgt tcgctgtccg cgtccaacac gccgatacag cggccgtctg
     3781 aaaacagcgg cacgacaatc tccgaacgcg acaaagacga acaggcaata tggtcgggat
     3841 gcgcgtccac atccccgacg accaccgtcc cgcccttcgc ccaagcctga ccgcacaccc
     3901 cgcgaccgaa cggaatccgc gtacacgcca aaggcccctg aaacggtgcc aaaaccaatt
     3961 cgtccgaacg cgtatcgacc aaataaaaac ccacccaaaa ccaaccgaac gcctccttca
     4021 aaaccgccgc cgtgttcgcc aaattcgcca cccaatccgt ctcgtccgcc accacagact
     4081 caatctgcgg caacacctcc cgataaagcg cggccttgtc cgaagccgaa aaatgaagcg
     4141 cgtgcatcac atctcctata gttgcataca tatcaggcgg ccattataaa acaacctgcc
     4201 cgaaacaaca ttccaaaccg cccccgcagc cgcccgcccc aagttgcgaa cccgccgcat
     4261 atccactaaa cttcacattg caacgcgcca cacgcggcag acagaaaaaa cacgacacgg
     4321 agcaaaaaag atgtatcacc aaatcggaat gtgggatcaa aaatgggtca tcggcaactg
     4381 gaaaatgaac ggccggctcc agaacaacaa ttcgctcatg caccgcttcc gcatcctccc
     4441 caccgccgaa cgcgtcctca tcggactcgc cgccccgacc gtttacctgc tgcaactgca
     4501 caacgccatg caaatcgtct taaacaaccg catcctcacc tgcgcccaag acgtgagccg
     4561 cttccccgac aacggcgcgt acaccggcga agtgtccgcc gaaatgctcg ccgacaccgg
     4621 cacagacatc gtcctcatcg gacactccga acgcagcctt tatttcggcg aaaaaaacga
     4681 aatccaacgc cgcaaaatgg aaaacgtcct caacgtcgga ctcatcccgt tattgtgcgt
     4741 cggcgaaagc ctcgaagagc gcgaagccgg caaagaacac gaagtcatcg cccatcagct
     4801 ttccatcctg caagggctgg ataccaaaaa catcgccgtc gcctacgaac ccgtctgggc
     4861 gatcggcacc ggcaaagtcg ccaccgtcga acagattgcc gatatgcacg cattcatcta
     4921 caaagaaatc ttgtctttgt gcggaagcga tgttaaaatc cgcgtccttt acggcggaag
     4981 tgtgaaagcg gacaacgcgg ccgacatctt cgcagtacct tatgtggacg gcgcactcgt
     5041 cggcggcgcg tcattgtcgt acgactcctt taccgccatc atcagtgccg cacaaaatgc
     5101 gtagaaaaat atggaagcct tcaaaaccct aatttggatt gttaatataa tttccgcttt
     5161 ggccgtcatc gtgttagtat tgctccaaca cggcaaaggc gcggatgccg gcgcgacttt
     5221 cggatcggga agcggcagcg cgcaaggcgt attcggctct gccggcaacg ctaacttcct
     5281 cagccgctcg accgccgttg cagcaacatt tttctttgca acctgcatgg ctatggtgta
     5341 tattcacacc cacacgacaa aacacggttt ggacttcagc aacgtacaac aaactcagca
     5401 agcacccaaa cccgtaagca ataccgaacc ttctgcccct gttcctcagc agcagaaata
     5461 acagtttttc aaatgccgac atggtgaaat tggtagacac gctatcttga gggggtagtg
     5521 gccgtaggct gtgcgagttc aaatctcgct gtcggcacca acacacaaaa cgcttagaaa
     5581 ttctaagcgt tttttttcgt ttaatcctgt ctaatgccgt caaccaatcc caatcaatac
     5641 aaggatttca ggctgattgt tatcctgccg tcccccttcc tgacagtgca atcccgtcca
     5701 atccgcccta attgaactaa cctgattttt tacagcatcc gaaaataccg cgagaaaata
     5761 ccgtaaaaat aaagctgaac gaccaccaaa tcaggaatgc caagcgggaa atagcttgcg
     5821 gggaatactg acgcaccgta gggaatgagg gggaaaccgt ccaagatgca gagcggtttt
     5881 ttttgggttt ttggaaaaaa cctatactat agtggattaa atttaaaccg gtacggcgtt
     5941 gcctcgcctt gccgtaccgg tttaaattta attcactata aaataagaaa aagataaaaa
     6001 attggtaaca aatgcggtaa caaatggtaa cgaatcggta acaacttttg gggttttccg
     6061 gtttttcacc gtcttggcag tgggagcgta gcggaatgaa aagccaaaac gcacggaacc
     6121 gcgcctattt tgagcaggaa tggcggttaa accgcttggt tatatacggg gcataggaag
     6181 acagcgaaac gcgctaaaaa cgcaatttga gccattaaaa gcgattgatt aaaaaataat
     6241 caggttagcc gccggtgttt caggcggcat aaacggagaa attgcggggc ataaaaaagg
     6301 cagcttgccg tgttgtctgt ctctggtata attccaagta tcactaatca acggctacac
     6361 aatgcggata ttcaaaaacc aatggatagt gaaatttgcc aagaagcaca aaatcaacga
     6421 ttccgagctg ctggaagcgg tagagcgggc ggataacggg ctgatagacg cagatttagg
     6481 cggcggtgtg attaagcagc gcatagcaag gcaaggacaa ggcagaagcg gcggctatcg
     6541 cagcctgata ttgttcaaac aggcagatag ggcatttttc gtttacgcct ttgccaagaa
     6601 cgacagggaa aacatttcgg ataaagaact ggacgtttac cgaaaagccg ccgcatatta
     6661 tctgaaatac acgcgggcag agctggcggc tttgaaagaa gacggcatta tcacggagat
     6721 agaattatga aatacaaaag cgaggcatta gccgccattc atgaaatgat ggaaggggct
     6781 tacaacatcg gcgcaatcga caaaaagact atgcgcggct ttgacaagtc ctgcctgacc
     6841 gaaatcaaac cgttgagcgg cggggacatc aaggcaatca gggagaagga ggcactatcg
     6901 caagccgctt tcgccatcta tctcaacgtg ggaaaaaatc acgtttcggc ttgggagcgg
     6961 ggcgttaaaa agccgagcgg cgcggcgttg aagctgctga ccatcgtcaa aaacaagggt
     7021 atcgaagcca ttgcgtagcc gacttggcaa acggcaaaat cagcaagttc acaatagacg
     7081 cgctgctgaa tatgcctgcc aagacaggca agaccgccga accgaatatc agggcatagc
     7141 cgcacaaatg cccgaccgca tcaaaccaag ccgaaacggc ggcggtgcag acggcatagc
     7201 ccgacagcaa ggcacggcgc agacgggcgc gaaacccgaa acatccccga ccgcgaggca
     7261 cggggatttt ttgcgcccgt tgcaggggga ttagatttaa gcggcgcggg cttgaaggca
     7321 aaacgggcgg ggcgcagaac tgtttaaatg ccgtctgaat ctcaaacgat ttcagacggc
     7381 attttgaaac aacggctcaa attctcgatc cccttccctt aacgccgacg ttttttatta
     7441 acgcgcccct tatttctgac actttgctca taaaccggca taacggtcgg caacaaccgt
     7501 tttagatttt ctatacaggc attgtttaca gcatcagcat aagacaccgt actctggaat
     7561 accgcctaaa ccctgcggat agttttggat gtggtatcga cattgtcggc agacttgttt
     7621 aactcgaccg ttttcaaata atctttgaca gatgctgcat tcagtgtcgc ggaatccgcg
     7681 tattttgaac aaaaggtcgg gctactttat cacaatagca gaaagatgga aaacggcggg
     7741 gattggtgaa aggtcgtctg aaatgatatt cagacgacct tgaacggttg tgttttaaat
     7801 atggtattta ttcatttgtt tcaccatcag gcagtccaaa ccaccttaag ggaatcgtgt
     7861 taaattggta atggcagtta acactgataa tataatctaa aacatcttta atattccggt
     7921 tttgtaaacc aatcattcaa aacatagaca tatccaaccc gccaacctaa agagtgaact
     7981 aactttgtat attgttttct cttaaaatcg caagtttgta atttctcatc tactgaaccg
     8041 ggagtttgtt ggaatttgat ctcaataata aataaggtat cacggatgat gataaataag
     8101 ccattatcgg gcagtaatct tttagacaga tggtctttcc aattaatatt gtattccagt
     8161 ctttccaaaa atcggtaaaa ctcatatttt ttgaaacaat aagccttcaa ttctccatta
     8221 aaccaaactt cataacccgc attatcatct gtttttctta agtcgtaccc gggaatttct
     8281 tcaaacaact ttcgtaagtc tgtacgttct tcgaatctga gtcctgtttg tgtccttgca
     8341 ccgccgattc cgccaataat catttttcta accttttctt tgctaattca aaaaaatctt
     8401 cttccagttc gcaaccgcaa aatctccgac catgttttaa ggcggcaacg cctgttgtgc
     8461 cgctgcccat aaaagggtca aaaattaaat ctccgatatt tgaagccgat agtatgcagc
     8521 gttcaagtaa ggggagtggt ttttgtgtcg gatgtttgcc gaatgttttt tcggttttat
     8581 ttggtggagg aaatgtccaa acacatttca tctgtttgcc atcattttgt gttttcatgg
     8641 tttcataatg aaacgtgtgt ttagcttttt tatttttctt tgcctataag attgtttctg
     8701 tcgaatgggt aaagaaacgg caggacaaat tagggggagg attgggtttt tcccaagtaa
     8761 tattgttcaa aatatggtag ccgacggttt gcatcaggta gccgattaaa tagatattat
     8821 gaaatgtgcc gcaaacccaa attgtgccgt ttggttttaa taaggcgtaa cacagtcgga
     8881 gccattcttc gtaaaattcc aaatctgccg ccattccttt ggatttatcc cagttgccct
     8941 tattgacgga aaccatttgc ccgttttgac aactgaaacc gtcattggaa agaaagtaag
     9001 gcgggtctgc gaaaatcata tcaaaacagc cgtttggatg tttttccaat atcttccgca
     9061 tgacgttaag ggaattttcg ttgtagagga caaggtcgtc tgaaacgatt ttttcaaacg
     9121 actttgataa atggcagctt cttcagccgg tttgtcaaaa tcaatcataa attgccaata
     9181 attctccgac ttttttacga ctgctgccct ttgcggcaat attgcgttgt acttcaaccg
     9241 tttttaaaat ggcagaagaa taaatatttc tcgtaaattc agtatcatga tttgaaatca
     9301 aaacgccttg ggagttgggt gcaatttgtc gatttttttg taaagtccgc gaccaatgaa
     9361 ttcgatcata ttttgatcac gcaaaatttg caactgttgg cggattttgt ctttgatatg
     9421 gttgttttgg ggaaattgga tggatagttt gttttcaaat tcatacattt gcgacaatgt
     9481 gaattcttcg gtgagttggt cgatacattt cataacagcc aaaagccagt ctttgcgctc
     9541 cgcattttgg ttgcgtaaaa acaggttgga ttgccatttt ttcagaacgg tttcgggttc
     9601 gataatgcgg gaatcgtcta ttaagaatat ttttccgctt tcaggcaaag gggcgagatt
     9661 gatggaacac ataatgtggt gcggtcggtt tttaatgcct ttatttctgg gaataatcat
     9721 atccggcgtg atgaaatgtt tgggtacaag caccaattgc cgtatggagt aatccgcttt
     9781 tttatatgca agaaagaaaa agttggggtt ggtatctgac cggatgcgct ccaacatagt
     9841 gcgatatgca ccgtcaggca cgctgttgcc tatggttttt tgatttttac tctttaattc
     9901 atattgctcg tggcaatttg ggcaaaagag gtctgcaaca ggtttgttat tggcaaatct
     9961 ctgcatcggc ttgcttccgc aacaggggca atagccgttt ttttccagcc aagcctcgct
    10021 cattacacgg attttgtggg ttgctttatt ttgttgcttt cccaattggg tatcgaaaaa
    10081 taaattcata ttttggattt tgagattaat ttttagtaga ttaacaaaaa tcaggacaag
    10141 gagacgaagc cgcagacagc agggcaaggc ggggcaacgc cgtaccggtt taaagttaat
    10201 ccgctatatt tgaggtctgt cttgcagaca acccaatctg ccttaaaaag gccgtctgaa
    10261 accctgtttc caagtttcag acggccttta tccgtatggc taaaccttaa aagcggttta
    10321 gacgacgatg ttcaccagtc tgcccggtac gacgatgatt ttcttcgcag gcttgccttc
    10381 catgaatttc actgcgcctt cgttggcgag tgcggcggct tcgaggtcgg ctttggaggc
    10441 gtcggcggcg acggtgattt tgccgcgcag tttgccgttg acttgaacca tcacttcgat
    10501 ttcggatttg accagggcgg cttcgtcgac tgtcggccag cctgcttccc acagtttcgc
    10561 gccgttcaat tcgctccaca gggtttcgca gatgtgcggc acgatgggcc acaacaggcg
    10621 tacggcggct tccaatactt cttgggcgac ggcgcggcct tgttcgctgc cggtgtcggt
    10681 tttgtcgtat tggttgagca gttccatcac ggcggcgatg gcggtgttga actgctggcg
    10741 gcggccgtag tcgtcgctga ctttggcggt ggtggaatgc agtttgtggc gcaggtcttt
    10801 gagttcttta gacaaaccgt cttggttgcc tgcaaatgct ttgaccgcgc cgccttgctt
    10861 caggtattcg taaacggtac gccacagacg gcgcaggaag cggtgtgcac cttcgacgcc
    10921 gctgtcgctc cattcgaggg actgttcggg cggtgcggcg aacatcatga acaggcgggc
    10981 ggtgtccgcg ccgtaggcgt taatcagttc ttgcggatcg acgccgttgt ttttggattt
    11041 ggacattttt tccgtgccgc tgatgacgac gggcagtccg tcggctttga ggacggcgga
    11101 aacggggcgg cctttgtcat cgaaagtcag ctcgacatcg gcggggttga tccagtcttt
    11161 gccgcctttg tcgttttcgc ggtagtaggt ttcgcagacg accatacctt gcgtgagcag
    11221 gcgttcaaac ggttcgtcaa cattgaccaa accttcgtcg cgcatcagtt tggtgaagaa
    11281 gcgcgcgtac aggaggtgca aaatcgcgtg ttcgatgccg ccgatgtatt ggtcgaccgc
    11341 gccccagtat ttcgcggatt ctgccgatac catgccgtct gaaaacttgg gcgacatata
    11401 gcggaagaag taccagctcg actccatgaa ggtgtccatg gtgtcggttt cgcgtttcgc
    11461 cgcgccgccg cagcacgggc aggaagtttc gtaaaactcg ggcatttttg ccagcggcga
    11521 acccataccg tcgggtacga cgttttcagg caggacgacg ggcagttggt cggcagggac
    11581 gggaacgtct ccgcattttt cgcaatggac gatgggaatc gggcagcccc agtagcgttg
    11641 gcgcgaaatg ccccagtcgc gcaggcggta ttgggttttc ggttcgcccg caccttggct
    11701 ttgcagcttg gcggcaacgg catcaaatgc cgtctgaaaa tccaagccgt ccaagtcgcc
    11761 gctgttgacc aatacgccgt tttctttgtc gccgtaccat tcttgccatc ggtttgcgtc
    11821 gaatgcgttg tcgccgacgg caatgacttg ttttttcggc agattgtatt tggcggcaaa
    11881 ctcgaaatcg cgttcgtcgt gcgccggaac agccatcacc gcgccgtcgc cgtagcccca
    11941 caagacatag ttggcaatcc acacttccag cttgtcgccg ttgagcgggt tgacgacgta
    12001 gcggccggtc ggcacgcctt ttttctccat cgtcgccata tcggcttcgg caaccgaacc
    12061 ggctttgcat tcggcgataa atgcctgcaa ttcgggtttg tcggcggctg cggctgttgc
    12121 cagcggatgt tcggcggcaa cagcgacata agtcgcaccc atcagcgtgt cggggcgggt
    12181 ggtataaact tgcaggaatt tcgcgtaatc gccttccaaa ccttgcttgc tgtcgtctga
    12241 aacggcgaag cgcacggtca tgccgcgaga tttgccgatc cagttgcgct gcatggtttt
    12301 gacttgttcc ggccagtgtt ccagcttgtc caaatcgttg agcagctctt cggcgtaatc
    12361 cgtgattttg aagtaataca tcgggatttc gcgtttttcg atcaacgcgc ccgaacgcca
    12421 gccgcgtccg tcgatgactt gctcgttggc aaggacggtt tggtcgaccg ggtcccagtt
    12481 caccgtgccg tttttgcgat agacgatgcc tttttcaaac agcttggtaa acagccattg
    12541 ttcccagcgg tagtattcgg gtttgcaggt ggcgacttcg cgttcccagt caaccgcaaa
    12601 acccaggctt ttgagctggg ttttcatgta ttcgatgttg tcgtaggtcc aagcggcggg
    12661 ggcgacgttg tttttcatcg ccgcattttc cgccggcatg ccgaacgcgt cccaacccat
    12721 aggctgcata acgttgaagc cgtttaaaag tttgaagcgg ctcaatacgt cgccgatggt
    12781 gtagttgcgt acatgcccca tatgcagctt gccgctaggg taagggaaca tcgaaaggca
    12841 ataatatttg ggtttggaag cgtcttcgga gacgttggaa atacgggcgt cgtcccattt
    12901 tttctgcgcc gcaggctcga tggcggcggg ctggtaatgt tcttgcatgg tcattttgtt
    12961 tttcgcttga aaacgttagg aatttaaagg aggaattata acaggttgcc gatgtcggcg
    13021 aatcggcaaa ttgccggcag gatgcgtaaa ttcgcacgcg catcattccg tatgccgtac
    13081 aaatacaccg cgtttactga tacgcacgtt ttttatgcta atattacgaa ccaaaatcaa
    13141 acgtttaaaa ctctcctgat gcggctcttc cgaacaaaag gcagacgggc atcgggtaaa
    13201 aaaggattct gcatatgaaa atcaaacaaa tcgtcaaacc gagcttggca gtattggcgg
    13261 caggcattct gtctgcctgc gcaaccataa agcaacgtca aagccgacgg cacgaccgac
    13321 aatccggttt tcccggaacc ctattccgta acgctcgaca acaagcgcgg cacattcccg
    13381 acttatgacg aactggatca gatgcgcccc ggtctgacca aagacgacat ctacaaaatc
    13441 ccgggccgcc cgcattacga cgaaggtatg tacggcgtgc gcgaatggga ttacctgttc
    13501 cacttccaca ccccgggcgt gggtatcgac cctgaaaaca cttccggcgt ggaaggcgtt
    13561 actacctgcc aatacaaagt gattttcgat aaagacaaat ttgcccgcag cttctactgg
    13621 aaccccgtct tcccgaaaga tgccgtctgc ccgccgcccg cacccaaagc cgagccgcaa
    13681 gtcatcatcc gcgaaatcgt gccggcaaaa ccgaaacgta tccgccaata agccgccatg
    13741 ccgccccgcc tgctttcagg gatattgtgc tgcctgctga cgactgcccc cgtattcgca
    13801 caggggcagc cggatgcggt cagtgcctat attcaaaaga aaaaagtcat cgtcgacaca
    13861 tccaaggcgg aactctgttt tgccgacgac cgtcagtgcc accccgtcct catcggcact
    13921 gccacaccca aggggacgtt cggactgacg ctgaacagta ccgacaagcc cggttacggc
    13981 ggcgaagtca tcggtttcaa gcaggagggc gattttcttt tcgccctgca ccgcgtttgg
    14041 aatcagatac cgtccgaaag gcgaaacgaa cgcatcgcct ccccgtccgt gtccgacagg
    14101 attatgacca acggctgcat caacgtcagc gatgcggtgt acgaaaaact gcgtcattat
    14161 tttgtgttgg aagtgatttg aaacagacgg ataccgcacg tgccggtatc cgttttcaca
    14221 ttgccccgat gcctgaaaca gaccgtccgt cacgtcatgc cgcctgaaac cggcgcagat
    14281 gccgccaagc cttcagacgg cattgtccgc ccgctccgac cgaacaacaa ccatctttgg
    14341 gagaacctta tgaccgaaca aaaccgcatc ctctgccgcg aactgagcct gctggcattc
    14401 aaccgccgcg tgttggcaca ggcggaagac aaaaacgtcc cccttttgga acgcctgcgc
    14461 ttcctgtgca tcgtgtcgtc caatcttgac gagttttttg aagtccgcat ggcgtggctc
    14521 aagcgtgaaa acaagctgca cccccggcgc aggccggaca acggcaaaac gccgtctgaa
    14581 accatcgccg acgttaccga agcggcgcgc tccctgatac ggcaccagta cgacctgttc
    14641 aacaacgtcc ttcagcccga gttggcgcgg gaaagcatcc atttttaccg ccgccgcaat
    14701 tggacgggca cgcagaaaaa atggatcgaa gactatttcg accgcgaact gctgccgatc
    14761 ctgaccccca tcggactcga cccttcccac cctttcccgc gcccgctgaa caaatcgctc
    14821 aacttcgccg tagaactcga cggcacggac gcgttcggca ggccttcggg tatggcgatt
    14881 gtccaagcac cgcgcatcct gccgcgcgtt gttcccctgc cgtccgaact gtgtggcggc
    14941 ggacacggct tcgtcttcct gtcgtccatc ctccacgccc acgtcggcaa actcttcccg
    15001 ggcatgaacg tcaaaggctg ccaccagttc cgcctcacgc gcgacagcga cttgaccgtt
    15061 gacgaagaag acgtacaaaa cctccgcgcc gccattcaaa acgaactgca cgaccgcgaa
    15121 tacggcgacg gcgtgcggct cgaagtcgcc gacacctgtc ccgcctacat ccgcgacttt
    15181 ctgctcgcgc aattcaggct gaccgacgcc gaactctatc aggtcaaagg cccggtcaac
    15241 ctcgtccgcc tcaacgccgt ccccgaccta gtcaaccgcc ccgatttgaa atttccgccg
    15301 cacacgccgg gccgtctgaa agccttgggc aaaaacagcc cgatcttcga tttggtgcgc
    15361 caatcgccca tcctgctgca ccacccctac caatctttcg atcccgtggt cgatatgata
    15421 cgcgaagccg ccgccgatcc cgccgttctt gccgtcaaaa tgacgattta ccgcaccggc
    15481 acgcgctccg aactcgtccc cgccctgatg aaggcggcac tcgccggcaa acaagtaacc
    15541 gtcgtcgtcg aactgatggc gcgttttgac gaagccaaca acgtcaactg ggcgaagcag
    15601 ctcgaagagg cgggcgcgca cgtcgtgtac ggcgtgttcg gctacaaagt ccacgccaaa
    15661 atggcactgg tcatccgccg cgaagacggc gtgctcaagc gttacgccca cctcggcacg
    15721 ggcaactacc accaaggcac atcgcgcatc tacaccgact tcggcctcat taccgccgac
    15781 gaacaaatca ccgccgacgt gaacacatta tttatggaaa tcacaggctt gggcaaaccc
    15841 gggcggctga acaaactcta ccaaagtccg tttaccctgc acaaaatggt tatcggccgc
    15901 atcgctcgcg aaaccgaaca cgcaaaagcc ggcaaaccgg cgcggattac cgccaagatg
    15961 aattcgctca tcgaaccgac tgtcatagaa gccctgtatc gggcaagcgc ggcaggcgtg
    16021 caaatcgatt tgattgtgcg cggtatgtgt accttgcgcc cgggtgtaaa aggcttgtcc
    16081 gaaaacatcc gcgtccgctc catcgtcggc aggcagctcg aacacgcgcg cgtatattgt
    16141 ttccacaaca acggcgcgga cgacaccttt atttccagcg cggattggat ggggcgcaat
    16201 ttcttccgcc gcatcgaaac cgccaccccg attaccgcgc ccgaactcaa aaaacgcgtc
    16261 atccgcgaag gtttggaaat ggcactggca gacaataccc acgcgtggct gatgcagccc
    16321 gacggcggct atatccgcgc cgcgcccgcc gaaggcgaaa gcgaagccga cctgcaaaac
    16381 gatttatggg atttgctccg aggctgaccc gcacaagcac ttcccccata cgctcccaac
    16441 cccaatccgc cgcattccag acggcattgc ctttccggca gccgaacaaa accccgaatc
    16501 cgtctgcacg gttcggggca tatctccaat acgggcatcg tgttccggaa aaccgtcaaa
    16561 tccgcatcgg catcacaata tatttgaaat tcggattgtt cggcacggta aacagcgtcg
    16621 agcggttggc gtcgccgaag gcaagctgca tatcgtcgga atggatgttg cgcaacacgt
    16681 ccatcaaata gccgatattg aaaccgactt cgagttcgcc gccctgatag gcgatttcga
    16741 tttcttcgcg cgcttcttcc tgctcgttgt tgctgcacac gacgctcaac aggccgggtt
    16801 gcaggaacag gcgcgcgcca cggaattttt cgttggcaag aatggacaca cgttccagcg
    16861 cgcccaaaag ttcggcacgg gacaacacga aaatcttgtc gttgtccaaa ggaatcacgc
    16921 ggttgaaatc ggggaatttg ccgtcgatga ccttgctgac gatggtcgtg ccgttgcatt
    16981 ggaaacgcac ctgcttgtcc agcagctcga tttgaatcgg atcgtcgggg ttgttcaaca
    17041 gtttgaacag ttccagcacc gttttgcgcg gcaaaatcac ttcggcgcgc ggcaaatccg
    17101 catcaatcgc gcaggctgca taggcaaggc ggtgtccgtc ggtcgccaca aggcgcaact
    17161 ggctgccctc aacctgcatc agcagaccgt tgagataata gcggatgtcc tgcaccgcca
    17221 tgctgtactg cacttgcgac agcatggttt tgaaacgctc ctgccccagc gagaaagtcg
    17281 cgctgatgtc ctcgccgaca ttcatcatcg gaaaatcggc ggcaggcagg gtttgcaggg
    17341 caaaacgcga tttgcccgcc ttcagcgtca gacggttgtc gtcccaatcc agcgacacca
    17401 gcgcaccggc aggcagcgcg cgcaaaatgt cctgaaattt cttggcattg gtagtgatgc
    17461 ggaagtcgcc cgcacctccc tcgggacccg cggtgtcgat ttggatttcc aaatcggttg
    17521 ccaagagttt ggtctgaccg cccctgccct caatcagcac attggacagg atgggcaggg
    17581 tgtgtcggcg ttcgacgatg ccggtaacgg cttgcagcgg cttgagcagg ctgtcgcgct
    17641 cggcttgtaa aatcaacatt ttcgctcctt aaatcggttg gatttcctac ccttcggacg
    17701 gcatggtcaa tgtcatgccg tctgaaaggc gtgtccggtc agttttgaat cagaatcagc
    17761 agtttctcgt aatcctgcgc caactcgggg tcttcctcgc gcagtttcgc caccgccctg
    17821 atgccgtgca tgacggtcgt atggtcgcgt ccgccgaacg aatcgccgat agacggcagg
    17881 ctcaaagtgg tcaattcttt ggtcaggctc atagcaacct gacgcggacg ggcaatgttg
    17941 cgcgtgcgtt ttttgccgag tacgtcgctg attttgatgc ggtaatattt cgccaccgca
    18001 tcgatgatga tgtcggcggt gatgactttg tgcttctcgg caataatgtc ctgcaaagcg
    18061 gtacgcgcca aatcgatgtc gatgacggga cggttcataa agcggctgct cgctccgaca
    18121 cggttgaacg cgccttccag ttcgcgcacg ttggaacgga tcagattggc aatgaacagc
    18181 gcggcttcgt cttcgatact gatgcccgcc gcttccgcct ttttctgcaa aatggcgata
    18241 cgcatttcca attcgggcgg ctcgagttcc aaagtcagcc cccacgaaaa gcgggatttg
    18301 aggcggtcgt ccataccttc gattttggcg ggtaaaacat cgcaagtgag gatgagctgt
    18361 tttttctcat tgtgaaaatg gttgtacaga tagaaaaatt cttccatcgt acggtctttt
    18421 cctttgatga actgaatatc gtcgataatc agcaggtcgt attgcttgta ttgctgcttg
    18481 aacacgtcgt aggtattgtt gcgaaccgcc ttcataaagc tgcggatgta gtcgtccgaa
    18541 tgcatatagc gcactttggc atcgggacgg tttttcaaca gctcgttgcc gaccgcctgc
    18601 acaaggtggg ttttgcccaa acccgtgctg ccgtacagga agaacgggtt gtaactctgc
    18661 cccgggtttt ccgcaatcgc ctgcgccgca gccgccgcaa ggcggttgcc cttaccttct
    18721 accaacgtat caaacgtgta atccggagac aggttggttt gttcgtaacg cgcttcttcc
    18781 gcatcacgct gcgcatccgt ccgcgctttg gcaactgccg ccgattccgg ccgggaagca
    18841 ggcccggcag cctgacgcgg ctcgtgcggc aggtttttca tacgttccgc caaaatatcc
    18901 gccgccgttt tcgacgcagc gggtttgaca ggcttttcag acggcagctc gtccaacaga
    18961 acctcctgca cgggcatctc ctccgacccc gcgtgcaagg acggctcggc aggttcgaca
    19021 gcaccttcaa ccgccgccat ctcataacgc acgccttctc ccggtttgaa tacgaaggcg
    19081 ggacggccgg cagccaactc ttccctcacc gcttctattt ttccggcaaa ctggctcttg
    19141 agcatattgc aggcaaactg gttcttgccg tacaccaccc atacgccacc ctcctcacca
    19201 accgtaaggg gcgcaatcca ttgcgcaaac tgcccgtgag gcaacatatc gtgaagacgg
    19261 cggaggcaca gcggccaaaa ctctgctaat gtcatggata ggctcgaatc ggtaaaaatg
    19321 aaatcgaaaa caaagaaaat ataatatttt caaaaagaaa acaaatctgt tgaatacgcg
    19381 tcggttcaaa acgcagcgaa ccgattatac cgactcgccg atattttatc cacaacccgt
    19441 gcaaaaattt atccacagaa aggcggcaga aatccgcagg caatcgggca atcctcctgc
    19501 aaagtttcta tattgattga caaaagcggc aaattggagt gtaattcacg gtttaattat
    19561 ctacccattc tattttagga accatcatga aacgcactta tcaaccttcc gttaccaaac
    19621 gcaaacgcac ccacggcttc ctcgtgcgct ccaaaacacg cggcggacgc gcagtattgg
    19681 ccgcacgccg tgccaaaggc cgcaaacgcc tggcagtgta attttggact accgcttcgg
    19741 aaggcagtac cgcttgttaa aaacggatga tttttcatcc gtttttgcat tcagaaaccg
    19801 ccgcagccgc gacctgctgc aagtttcgcg ctcaaacggc aacgggctgg accatccccg
    19861 catcggtctg gtggtcggca aaaaaaccgc caaacgcgcc aacgagcgga actatatgaa
    19921 acgcgtcatc cgcgactggt tcagattgaa caaaaaccgg ctgccgcctc aggatttcgt
    19981 cgtgcgcgtc cgccggaaat tcgacagggc taccgcaaaa caggcaaggg cggaactggc
    20041 acaactcatg ttcggcaacc ccgcaaccgg atgcgggaaa caggtatgat cagaacggta
    20101 ccctgcaggc aaggttcaga cggcaacggg tttcccatac aaggaacatc ccgatgaact
    20161 tcctattgtc caaactgctg ctggggctga taaggtttta ccaatactgc atcagcccgc
    20221 tgattccgcc gcgctgccgt tatacgccga cctgttcgca atacgcggtc gaagcggtca
    20281 aaaaatacgg cgcattcaaa ggcctccggc tcgccatcaa gcgcattgca cgctgccacc
    20341 ctttcggcgg acacggacac gaccccgttc cctgacccga cgcaatattc aaattgcacg
    20401 ctttcctttt atttcccatc ggtttctata taatgccgtc tgaaccttcg ggctggcggc
    20461 acggccgtcg ggtatgaagc ccgcccttat tccccgtcta tcggaacacg caacctgcgg
    20521 catttccgac cattcaggaa actctatatg gattttaaaa gactcacggc gtttttcgcc
    20581 atcgcgctgg tgattatgat cggctgggaa aaaatgttcc ccaccccgaa acccgtcccc
    20641 gcgccccaac aggcggcaca aaaacaggca gcaaccgctt ccgccgaagc cgcgctcgcg
    20701 cccgcaacgc cgattaccgt aacgaccgac acggttcaag ccgttattga tgaaaaaagt
    20761 ggcgacctgc gccggctgac cctgctcaaa tacaaagcaa ccggcgacga aaacaaaccg
    20821 ttcgtcctgt ttggcgacgg caaagaatac acctacgtcg cccaatccga acttttggac
    20881 gcgcagggca acaacattct gaaaggcatc ggctttagcg caccgaaaaa acagtacacc
    20941 ctcaacggcg acacagtcga agtccgcctg agcgcgcccg aaaccaacgg actgaaaatc
    21001 gacaaagtct atacctttac caaagacagc tatctggtca acgtccgctt cgacatcgcc
    21061 aacggcagcg gtcaaaccgc caacctgagc gcggactacc gcatcgtccg cgaccacagc
    21121 gaacccgagg gtcaaggcta ctttacccac tcttacgtcg gccctgttgt ttatacccct
    21181 gaaggcaact tccaaaaagt cagcttctcc gacttggacg acgatgcgaa atccggcaaa
    21241 tccgaggccg aatacatccg caaaaccccg accggttggc tcggcatgat tgaacaccac
    21301 ttcatgtcca cctggatcct ccaacctaaa ggcggccaaa gcgtttgcgc cgcaggcgac
    21361 tgccacatcg acatcaaacg ccgcagcgac aagctgtaca gcgcaagcgt cagcgtgcct
    21421 ttagccgcta tccaagccgg cgccaaagcc gaaactgccg tcaacctgta tgccggtccg
    21481 caaaccacat ccgttatcgc aaacatcgcc gacaacctgc aactggcaaa agactacggt
    21541 aaagtacact ggttcgcatc gccgctcttc tggctcctga accaactgca caacattatc
    21601 ggcaactggg gctgggcaat cgtcgttttg accatcatcg tcaaagccgt actgtatcca
    21661 ttgaccaacg cctcctaccg ttcgatggcg aaaatgcgtg ccgccgcacc caaactgcag
    21721 accatcaaag aaaaatacgg cgacgaccgt atggcgcaac agcaagcgat gatgcagctt
    21781 tacaaagacg agaaaatcaa cccgctgggc ggctgtctgc ctatgctgtt gcaaatcccc
    21841 gtcttcatcg gcttgtactg ggcattgttc gcctccgtag aattgcgcca ggcaccttgg
    21901 ctgggctgga ttaccgacct cagccgcgcc gacccctact acatcctgcc catcattatg
    21961 gcggcaacga tgttcgccca aacctatctg aacccgccgc cgaccgaccc gatgcaggcg
    22021 aaaatgatga aaatcatgcc gttggttttc tccgtcatgt tcttcttctt ccctgccggt
    22081 ttggttctct actgggtggt caacaacctc ctgaccatcg cccagcagtg gcacatcaac
    22141 cgcagcatcg aaaaacaacg cgcccaaggc gaagtcgttt cctaaatgcc gcagcatgaa
    22201 aaatgccgtc tgaaacctgt tcagacggca tttttattgt tcacccccta tcggggcgga
    22261 aatcttcaac ccgcatacat cacaaaaatc gtcgggcgtt ttttcagatt gggcatttcc
    22321 ttcatcctgc gccaatccgc aaccgtttta ctgaccacca actgagtcgg caacgtcaaa
    22381 tccgtagccg tacacaagcg cgtttcagga tgcaggtttt ccaccgcatc ggcaagcagc
    22441 gcatcattgc ggtaaggcgt ttcaataaaa atctgcgtct cgccgcactg ccgcgaacgt
    22501 tgctccaaag cattcaaact ctgaatgcgc tcattttttt cagacggcag ataaccatta
    22561 aacgcaaagt tctgcccatt cgcacccgaa gccatcagcg ccagcagcag gctggacggg
    22621 ccgaccaaag gccgtacttc aaaaccgtgt ttatgcgcca atgccaccaa attcgcgccc
    22681 ggatcggcaa cagccgggca acccgcctcg ctgacaatcc ccatactgcg cccttcttgc
    22741 aaaggtttca gcaattccgg caaagtcttc aaatccgtgt gttcattcaa cgtttgcaga
    22801 ttcagttcgc gtatcggcgt cgttatcccc aaatgcttca aatgcgcacg cgccgttttt
    22861 tccgcttcca cgacaaaatc cgtcagcccg acaaccgccc gttgttcatg ctgcaacagg
    22921 cacggcgtgt caggcgtacc caaaggcgta ggaatcaaat acaaaacagg agacatcatt
    22981 ccctcactca tcggttaaaa atgccgtccg aacccttcag acggcataaa cgggcagtta
    23041 caaaacatcc acgccctcgt ttttcaaaaa atcgaccaga cggaaaaccg gcagaccaat
    23101 caaggcattc ggatcggtac tttcaatccg ttcgatcaac agcgcgccca agccctcgct
    23161 tttcgccgca cacgaacaat aaaccgcatc cggctcgcgc tccaaatagc ggaggatgtg
    23221 cagctcgtcc aactgcctca tcacgaccac cgtcttatcg atatgccgat gcatcctgcc
    23281 cgtaaccgta tttagcagaa cgaccgcact gtaaaactca atctccctgc cgctcaaatg
    23341 catcaacatc ttttgcgcgt tggcaaggtt catcggcttg ccccactgcc tgccgtcgca
    23401 ccacgccacc tggtccgcac cgacaatcaa cgccccgggg aaacgcccgg tcaacgaccg
    23461 cgccttaccc tcggcaaggc gcaatgccgt ctgaggggcg gattccccca acatcggcgt
    23521 ttcgtcaaaa tcgggggatg ccgcctgaaa ggcaatgccg agcctttcca tctgttcgcg
    23581 gcggaaaacc gaactcgtac ccaaaaccaa aggcagttcc aaacccatcc catcctcctt
    23641 accgttgaaa acacgcccga aggggcggta aaatccagcc atgcgccgaa acacggacgc
    23701 ccgccttcgg cgtaccgcaa catttttctt aaaaatattg acgttagaac atctaaatta
    23761 tatcataccc cgtttatgtc agaccctaat ttgattgacc cggaaatttt tgccgccgaa
    23821 aggcagaacc tgcaaggcag ttttctgctg gaagaattgg acgagcgagt cagtttgcac
    23881 gattatcccg ccgacaggcg gaccaaaata tcgtttacac tgaccggcgg tcgcgaccgg
    23941 ctgcaacgcc tgttcctcga cctgaacgtc aaagccgata tgcccctgat ttgccagaga
    24001 tgtatcaaac ccatgccgtt catgctcgat gaaagcagcc gtatcgtcct gttttccgac
    24061 gaagagtcct tggacgaatc catgcttgcc gacgaagaac tcgaaggcat actgattgaa
    24121 aaagaactcg acgtgcgcgc attggtagaa gaccaaatcc tgatgtccct gcccttttcg
    24181 ccgcgacacg gacactgcgg caataccctt ccggaatccg ccaaccaaga caaacccaac
    24241 ccctttgctg ttttggcggg tttgaaaagc agttaattag gacacagttt atttatctag
    24301 gagcttgaaa tggccgttca acaaaacaaa aaatcccctt ccaaacgcgg tatgcaccgt
    24361 tcgcacgacg cactgaccgc gcccgcactg tttgtcgaca gcacaaccgg cgaagtacac
    24421 cgcccgcacc acatctcccc caacggtatg taccgcggcc gcaaagtggt caaagccaaa
    24481 ggcgaataat ccctattcga ctgactgaaa aagccagaac attgccatgc aattactggc
    24541 tttttttaca ttggacgcac catccgtcca aactttcgcc atacgtcaac acacaggggc
    24601 aaagcgttcc gtataatacc ccgtgaaaat attccaaaag ccccaaccac caaggaaatt
    24661 ccgatgaaac agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg
    24721 gcagaaaaaa tccggaacgc cggcgtcaaa tacgatgcca tgatcgctat cggcggcggc
    24781 ggctttattc cggcacgcat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc
    24841 accgcctatt acgacagcga caacgaagga caagttaccg aagaagtcaa aaaagtccaa
    24901 tggctagacc ccgtccccga agtcctgcgg ggcaaaaacg tgctcgtcgt cgatgaagtg
    24961 gacgacagcc gcgtaaccat ggagttctgc ctgaaagaac tgctcaagga agacttcgac
    25021 actgtcggcg tcgccgtcct gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa
    25081 ggcattccct atttcagcgg cattaccgta gaagactggt ggatcaacta tccgtgggac
    25141 gcactcgaca tcgacgaaca caaccgcctt gccgaagccg gccgaggcta accctttcag
    25201 acggcatatt ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa
    25261 aatctaccgg agaaacccct atgattacat tggccgtaga tgccatgggc ggcgaccaag
    25321 gacttgccgt taccgtaccc ggcgcaaccg cattcctcca agcacacccc gatgtccgcc
    25381 tgattatgac cggcgacgaa acgcagctcc gccaagccct gaacgcggca ggcgcgccga
    25441 tggaacgcat cgacatctgc cataccaccc aggttgtcgg catggatgaa tcccctcagt
    25501 ccgccctaaa gaacaaaaaa tactcctcca tgcgcgttgc catcaaccaa gttaaagaag
    25561 gcaaagccca agccgccgta tccgcaggca acacgggcgc gctcatggca accgcacgtt
    25621 tcgtcctcaa gaccataccc ggcatcgaac gccccgccat cgccaaattc cttccttccg
    25681 acaccgacca cgttaccctc gccctcgacc tcggcgcaaa cgtcgactgc acacccgaac
    25741 agcttgccca atttgccgtc atcggcagcg aactcgtcca cgcactccat cctcaaaaag
    25801 gacagccgcg cgtcgggctg gtcaacgtcg gcacagaaga catcaaaggt acggataccg
    25861 tcaaacaaac ctacaaactg ctgcaaaaca gcaaactcaa ctttatcggc aacatcgaaa
    25921 gcaacggcat cctgtacggc gaagcagatg tcgtcgtcgc cgacggcttt gtcggcaacg
    25981 tcatgctcaa aaccatcgaa ggcgcagtca aattcatgag cggagccatc cgccgcgaat
    26041 tccagagcaa cctgttcaac aaacttgccg ccgttgccgc cctacccgcc ctcaaaggct
    26101 tgaaaaacaa actcgatccg cgcaaattca acggggccat cctgctcggg ctgcgcggca
    26161 tcgtcatcaa aagccacggc ggcacagaca aaaccggttt ccgctacgca ctcgaagaag
    26221 cctaccacga agccaagtcc gccggccttt ccaaaatcga acaaggcgtt gccgaacaac
    26281 tcgccgcact ggaagccgcc caaaacgaaa ccgccgccag tctgtaacac gcacgatgcc
    26341 gtctgaacgc ccccgcccct ttcagacggc atccggcacc cgtccaaacc tgcgggcgcg
    26401 gacggcgatg cgcctgtccg gcacttccca aatacttccc tgtaaaataa ggagtatttg
    26461 aaaaatgaag acattagaaa aacggatgaa agctctagac aaacggatga tgaagttcgg
    26521 aaaatccctt gaaggcaggc ttgatgcccg tctgattgaa tccgcattgg attatattca
    26581 ttattcggaa cgttttttgg cttttgacat cctgtgtact tatatcgaag atttcgatat
    26641 ccggctgacg gaacaagaat cccgggaaat ttcttttatc aacaaggaat ttgagataga
    26701 aagcacgtcc gattaaccaa taaagccaat gatttgataa gaatgaaaac atcgacggtc
    26761 gtttttggcg gattttttat ggcagacaac ggggagcgaa tccaaatccc cgttttggaa
    26821 aatcctgaca ttagggaaat caatcgcttt ttttccgtat caaattttga gaaaaaagcc
    26881 ggcgtccttg ttttcagaat catccccgag ccggaatttg gcaataccga attaactgtc
    26941 tattttgaaa aaggatatta tagtggatta acaaaaaccg gtacggcgtt gccccgcctt
    27001 ggctcaaaga gaacgattcc ctaaggtgcc caagcaccaa gtgaatcggt tccgtactat
    27061 ccgtactgtc cgcggcttcg ccgccttgtc ctgatttttg ttaatccact atatttgcca
    27121 ataattcaaa caattttaga aggcggggat attaaagtaa aaaaccttaa aacagaaaat
    27181 tacagcggga aggcaaggga aatcttggga gattcctacc ctattgaaca tatctctaaa
    27241 aatatttcta caattcaaaa tatcatttcc gaatttatca gacaaaaaca aacaccggca
    27301 ccaaataatt tgatggaaac ccaacaatca agatgccgtc tgaagcagct tgggctttca
    27361 gacggcattt ccttcactta aaacagcgta tcggcaaccc cgccccgccc gtccacggca
    27421 atccgcatct gaaaaccatc tgtatgccaa accacacccc catccctgtt tccgtcattt
    27481 gcaccctgcc cgtattgggc aatcatcggt ttttcgctta caatagccga atctgaacca
    27541 actctctaaa aaggccgttc ccatgcagta tgcaaaaatt tccggcacgg gcagctatct
    27601 tcctgccaac cgcgtcagca atgacgacct tgcccaaaag gtagatacct ctgacgagtg
    27661 gattaccgcg cgtacgggca tcaaattccg ccatattgca gccgaaaacg aaaaaaccag
    27721 cgatcttgca gccgaagcgg cgcggcgcgc tttggcagat gccaaattaa atataaacga
    27781 cattgatttg attatcgtgg cgacggcaac gccggatatg cagtttcctt ctaccgccac
    27841 catcgtgcag caaaaattgg gcatcaccaa cggctgcccc gcgtttgacg tgcaggcggt
    27901 gtgcgcaggc tttatgtacg cgctgactac ggcaaatgcc tacattaaaa gcggtatggc
    27961 gaaaaacgcg ctggtcatcg gcgcggaaac cttcagccgc atcgtcgatt ggaacgaccg
    28021 cacaacctgc gtgctgttcg gcgacggcgc gggcgcggtg gttttaagcg cgtccgacaa
    28081 accgggcatc atccacagca aactcaaagc cgacggcaat tatctgaaac tcttaaacgt
    28141 ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg tacatttcga tggacggtcc
    28201 cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc gccgatgacg ttatcgaaga
    28261 agcaggttac accgccgaac aaatcgactg gattgttccc catcaggcaa accgccgcat
    28321 tatcgaatcg accgcgaaac atttgggttt gagtatggac aaagtcgtcc tgaccgtcca
    28381 agaccacggc aacacgtccg ccgcatccat tccgctggct ttggacgcgg gcatccgcag
    28441 cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc ataggcggcg gtttcgcgtg
    28501 gggcgcggtg ctgttgcaat attgaaaccg gtgccgtctg aaacaggctt tcagacggca
    28561 tttcccatat catgaagcgg caggctttct tcaaaccgat ggcgtgtgcg gcatttctgt
    28621 ccgccgtttc gctgcgcctc cccgtattgg gcgcgtgtta cgcaatattg tccctctatg
    28681 cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg gggaaaacgc cgcattcccg
    28741 aacaccgcct gctcctgcct gccttgttcg gcggttggac gggcgcatac ttgggtagta
    28801 ggatgttcag gcataaaacg gcgaaaaagc gttttgttgt gctgttccgt ctgactgttt
    28861 cgggcaatgt cctggcgacc tgcatcctga ttgattattt cgttccgccc gaactttttg
    28921 taaaactcgg gcaacatctc tgacaatcca acccaacaaa ggaatactat gtctttcgca
    28981 ttcttcttcc ccggacaagg ttcccaaagc ctcggcatga tgaacggctt tgccgaacac
    29041 gccatcgtca aaaacacatt tgacgaagcc tccgccatat tggggcagga cttgtgggcg
    29101 atgattaacg gcagcgatgc cgaaatcatc ggtcaaacag tcaacaccca gcccattatg
    29161 ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag tgggcggcaa aacgcctgcc
    29221 gccgttgccg gacacagcct cggcgaatac accgcgcttg tcgccgccga agcattggat
    29281 tttgccgatg ccgtcaaact cgtccgcctg cgcgccgaac tgatgcagtc cgccgtaccg
    29341 caaggcgtag gcgcaatggc ggccattttg ggtttggaag atgaacaggt caggcagatt
    29401 tgtgccgaat ccgcacaggg cgaagtggtc gaagccgtca acttcaactc gcccggacaa
    29461 gtcgtgattg caggcaacgc cgccgccgtc ggacgcacca tggctgccgc caaagaagcc
    29521 ggagccaaac gcgccctgcc gctgccggta tccgttcctt cccattgcag cctgatgaaa
    29581 cccgccgccg acaaacttgc cgaagcactc aaaaccgttg aaatcaagca gccgcaaatc
    29641 cgtgtcatcc acaacgccga cgttgccgcc tacgatgatg ccggcaaaat caaagacgcg
    29701 ctcgtccgcc agctttacag ccccgtgcgc tggacggaaa ccgtcaacgc ccttgtttca
    29761 gacggcattg ccgaatccgc cgaatgcggt ccgggcaaag tgttggcggg cttggcaaaa
    29821 cgcatcaaca aggccgccgc ttgcagcgca ctgaccgatg ccggacagat taccgccttt
    29881 atcgaagcgc actgacttcg ttccgcaaaa agcagcctgc cctcttcagg ctgcttttca
    29941 cgtccgaacg gcaaacagcc gcatatttgc gctataattc atccctacca aaccaccgac
    30001 agcggctgcc gttgcagttc ccgccctacc gatatgatag aaaaactgac tttcggactg
    30061 tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg cgtacgtccg cccctacaaa
    30121 atccgcatcg ttgccgccct gattgccatt ttcggcgttg ccgccaccga aagctacctc
    30181 gccgccttca tcgcccccct gatcaaccac ggcttttccg cacccgccgc gccgcccgag
    30241 ctgtccgccg ccggcatcat ttccaccctg caaaactggc gcgaacagtt tacctatatg
    30301 gtttggggga cggaaaacaa aatctggacc gtccccctct tcctcatcat cctcgtcgtc
    30361 atccgtggca tctgccgctt taccagcacc tatctgatga cttgggtctc cgtgatgacc
    30421 atcagcaaaa tccgcaaaga tatgtttgcc aaaatgctga ccctttcctc ccgctaccat
    30481 caggaaacgc cgtccggcac cgtactgatg aatatgctca acctgaccga acagtcggtc
    30541 agcaacgcca gcgacatctt caccgtcctc acgcgcgaca cgatgatcgt taccggcctg
    30601 accatcgtcc tgctttacct caactggcag ctcagcctca tcgtcgtcct gatgttcccc
    30661 ctgctctccc tgctctcgcg ctactaccgc gaccgtctga aacacgtcat ttccgactcg
    30721 caaaaaagca taggcacgat gaacaacgtg attgccgaaa cccatcaggg acaccgcgtc
    30781 gtcaagctgt tcaacgggca ggcgcaggcg gcaaaccggt tcgacgcggt caaccgcacc
    30841 atcgtccgcc tcagcaaaaa aatcacgcag gcaacggcgg cacattcccc gttcagcgaa
    30901 ctgattgcct ccatcgccct cgccgtcgtc atcttcatcg ccctgtggca aagccaaaac
    30961 ggctacacca ccatcggcga atttatggca ttcatcgtcg cgatgctgca aatgtacgcc
    31021 cccatcaaaa gccttgccaa catcagcatc cctatgcaga cgatgttcct cgccgccgac
    31081 ggcgtatgtg catttctcga caccccgccc gaacaggaca aaggcacgct cgcgccgcag
    31141 cgtgtcgaag ggcgcatcag cttccgcaac gtcgatgtcg aataccgttc agacggcatc
    31201 aaagccctcg acaacttcaa cctcgacatc agacaaggcg aacgcgtcgc cctggtcgga
    31261 cgttccggca gtggcaaatc caccgtcgtc aaccttctgc cccgctttgt cgaaccgtct
    31321 gccggcaaca tctgcataga cggtatcgac atcgccgaca tcaaactcga ctgcctgcgc
    31381 gcccaattcg ccctcgtctc ccaagacgta ttcctgtttg acgacaccct gtttgaaaac
    31441 gtccgataca gccgtcccga cgctggcgaa tccgaagtcc tgtccgccct ccaagccgcc
    31501 aacctgcaaa gcctgatcga cgcatccccg ctcggactgc accagcccat cggatcgaac
    31561 ggcagcaact tatccggcgg acagcggcaa cgcgtcgcca ttgcccgcgc cattttgaaa
    31621 gacgcgccga tattattatt ggacgaagcc accagcgcat tggacaacga atccgaacgc
    31681 ctcgtccaac aagcgctcga acgcctgatg gaaaaccgca ccggcatcat cgtcgcccac
    31741 cgcctgacca ccgtcgaatc cgccgaccgc atcatcgtca tggatggcgg caaaatcatc
    31801 gaacaaggca cacacgatca actcatgttc caaaacggct actacaccat gctgaggaac
    31861 atctccggaa aagataccgc cgccgttcag acggcataaa caaaatgccg tccgaaatgg
    31921 tataatcgcc ccgacccctt cagacggcac tatatccgcc gacccatccg attatcttca
    31981 accaccgtaa aacccattat gacccaagac aaaatcctca tcctcgactt cggttcgcaa
    32041 gttacccggc tgatcgcccg ccgcgtgcgc gaagcacacg tttactgcga gctgcattcc
    32101 ttcgatatgc ctttggacga aatcaaagcc ttcaacccca aaggcatcat cctttccggc
    32161 ggccctaatt ctgtttacga atccgactat caagccgata ccggtatttt tgatttgggc
    32221 attccggttt tgggcatctg ctacggcatg cagtttatgg cgcaccactt gggtggcgaa
    32281 gtgcagcccg gcaaccagcg cgaattcggt tacgcgcaag tcaaaaccat cgacagcgga
    32341 ctgacacgcg gcattcaaga cgacgcgccc aacacactcg acgtatggat gagccacggc
    32401 gacaaagtgt ccaaactgcc cgacggtttc gccgtcatcg gcgacactcc gtcctgcccg
    32461 attgccatga tggaaaacac cgaaaaacaa ttctatggca tccagttcca ccccgaagtt
    32521 acccacacca aacaaggccg cgccctgttg aaccgctttg tcttggatat ttgcggcgcg
    32581 caaccgggct ggacgatgcc caactacatc gaagaagccg ttgccaaaat ccgcgaacaa
    32641 gtcggcagcg acgaagtgat tttaggtctg tccggcggcg tggactcttc cgtagccgcc
    32701 gcgctgattc accgcgccat cggcgaccaa ctgacctgcg tgttcgtcga tcacggtttg
    32761 ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg cccgcaactt gggtgtgaaa
    32821 gtgatacacg tcgatgccga agggcagttt atggcgaaac tcgccggcgt gaccgaccct
    32881 gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag tatttgatgc cgaagagaaa
    32941 aaactcacca acgccaaatg gctggcgcaa ggcacgattt accccgacgt aatcgaatcc
    33001 gccggtgcga aaaccaaaaa agcccacgcc atcaaatccc accacaacgt cggcggcctg
    33061 cctgaaaata tgaagctcaa actgcttgag cccttgcgcg acttgttcaa agacgaagtg
    33121 cgcgagttgg gcgtggcttt gggcctgccg cgcgaaatgg tgtaccgcca ccccttcccg
    33181 ggccccggtt tgggtgtgcg catcttgggc gaagtgaaaa aagaatacgc cgacttgctg
    33241 cgtcaggcgg acgatatttt catccaagaa ttacgcaata ctaccgacga aaacggcacg
    33301 tcttggtatg acctgaccag ccaggcattt gccgtgttcc tgcccgtcaa atccgtcggc
    33361 gtgatgggcg acggccgcac ttactactac gtcgtcgcac tgcgcgcagt catcaccagc
    33421 gactttatga ctgcacactg ggcagagctg ccatactcac tgctcggccg cgtgtccaac
    33481 cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt acgatgtcag cggcaaaccg
    33541 cccgccacca tcgagtggga ataaacagca aacagggctg caccgtccgg cgcagtcctt
    33601 cgattatcgg aaaaaaggaa aaaatatgag cacacaagat ttaagcggca aaatcgcttt
    33661 ggtaaccggc gcatcgcgcg gcatcggcgc ggcaattgcc gacacactgg cggcagccgg
    33721 tgccaaaatc atcggtacgg caaccggcga gagcggtgcg gcggcgatta gcaaacgtct
    33781 ggcacaatgg ggcggcgaag gccgcgtatt aaattccgcc gaacccgaaa ccgtcgaaaa
    33841 cctgattgcc gacatcgaaa aaacgttcgg caaactcgac attctggtca acaacgccgg
    33901 catcacccgc gacaacctgt tgatgcgtat gaaagaagaa gagtgggacg acatcatgca
    33961 ggtcaacctc aaatccgtgt tccgcgcttc taaagccgtc ttgcgcggca tgatgaagca
    34021 gcgcgccggc cgcatcatca acatcacctc cgtcgtcggc gtgatgggca atgccgggca
    34081 aaccaattat gccgccgcaa aagcgggctt gatcggtttt gccaaatcta tggcgcgcga
    34141 agtcggcagc cggggcatta ccgtcaactg cgtcgccccg ggctttatcg ataccgatat
    34201 gacccgcgcc ctgcccgaag aaacccgcca aacatttacc gcccaaaccg ccttgggcag
    34261 attcggcgac gcgcaagaca tcgccgatgc ggttctgttc ctcgcttccg atcaggcgaa
    34321 atacatcacc ggtcaaacgc tgcacgtcaa cggcggtatg ctgatgccct aacggacagg
    34381 cttttcaacc atgccgtctg aagccctttc agacggcatt tgcattccca aacaaaatga
    34441 acactcacca ccacctgccc tgcccctgcg gttcaggcac aagctatgcc ggctgctgcc
    34501 gaccgcttca tctgcgccaa atcctgccgc ccaccgccga agccctcatg cgttcgcggt
    34561 acggcgcgta tgtgctgcac cttatcgact acatcatcgc tactaccgtt ccggcgcaac
    34621 aaacattcct cgatgccgcc gaactgatgc agtggagcag agaagccgaa tggctgggct
    34681 tgaatgtcat cgcacaccgc aacctcggca aacagcacgc ccaagtcgaa tttgaagcct
    34741 acttccaaga cggcggaaac cgaacagtgc atcacgagct gtcggcattc gtgaaaatag
    34801 cggagcaatg gtatttcatc gatccggctg ttccgctgcc taccatgaaa cagccctgca
    34861 tttgcggttc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgcaa cctgtcgcat
    34921 aaaaatgccg tctgaacgtt cagacggcat tttcaacgcg caaaaaaaac cattcatacc
    34981 aagggtaagt atgaatggtc aatacattgc gggaaaacat cttacttgct gcactgccga
    35041 aaagggagaa acggcagcgt taaccagcaa aagcattgta cctttattaa tgttaaaaaa
    35101 cattaatttt gaaaatatat taacgaatat atctggaacc tactggtttt cccgtatcca
    35161 cccgacccag cgttcaaaca gcttcggttc gagcgcggcg acgaccgagc gtttgaacac
    35221 gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc ccgcctcctc
    35281 gaaaatcaac gcacccgcag cataatccca cagcttctgc ccgccgtgga cataaacatc
    35341 ataacgcccg cacgccagat aacaccagtc cagcgtgctg ctgcccatac tgcgtatcgt
    35401 cccgaaaggc gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca gatatttgat
    35461 ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag gcaggcgtgt
    35521 cccattaaga aacgcaccct gcccgcgttc ggcataaaaa cattcgccgc tgaccggatt
    35581 gtagattacg cccaattcgg cgcgcccgtt gcggacaaac gccaccgaca cggcaaaatg
    35641 cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc acagcccctt
    35701 ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt cctcgcccaa
    35761 catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaat gccgtctgcg cggcaatgtc
    35821 cgcctcgctc aacatcgaac cgtcttcctt gcggcgggac ggcgtattca aaaaacgcgg
    35881 cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta acacttccta
    35941 ctgtcctcat attttgaact tgcggcgcgc gaacgtataa tgtccgtttc catcacgccg
    36001 ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg cccagattct acctgcccga
    36061 aaacctttcc gtcggacaaa ccgtcgacct tcccgacaac atcgtccgcc acctcaacgt
    36121 cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca aggcacatac
    36181 cgcacggctg accgttttgg aaaaacaccg cgccgaagcc gaaatcctgc acgaagacac
    36241 aaccgacaac gagtccccgc tcaacatcac gctcatccaa tccatttcct ccggcgatcg
    36301 catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac agcccgtcat
    36361 cagcgaacgc tgcatcgtcc gcctcgacgg agaacgcgcc gccaaacgcc tcgcacgctg
    36421 gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc cccccgtact
    36481 gcccatcatc ggctaccgtg aagcactcga caaaatgccg tctgaaagca ccaggctgat
    36541 tatgagcatc aaccgcgcct gcaaactcgg cgacatccgc cacccgtccg gcgcaatcgt
    36601 ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg catttgaagc
    36661 cggctttcag gcggttacac tcggaaaacg gattttacgc acagaaaccg ccccactcgc
    36721 cgccatcgcc gcgatgcaga cgctttgggg cgattttacc tagcagatga aaaaacacgg
    36781 gattttatcc gaaaaagcaa caatcccccc gccgtcattc ccgcgaaagc gggaatctag
    36841 aaatttaacg ttgcggtgat ttatcggaaa tgactgaaac ccaacgaacc ggattcccac
    36901 tttcgcggga atgacgaagt ggaagttacc cgaaacttaa aacaagcgaa accgaacgga
    36961 ctggattccc gcctgcgcgg gaatgacagt gtgtccattt ctgattttaa tcccctatat
    37021 tttacacaaa ctatttgaac aatatgaccc gcctgccgta aactttctca agctccgcct
    37081 gcctttgact ctccattctt ttcttctttt ccctaccgaa tttacccaaa gcacgcttca
    37141 agtcaaacat cgccttcaac gaacggcggt gtcttcttcc ttgttcccta tctttttcca
    37201 aatcgctacc caacatactg ttttgactga gaaacttggc ataatgcaat tcttgggtac
    37261 ataaggcggg actaacctga taaacaggca tcccctcctt atcaaagaaa taagtaaaca
    37321 tcatcaaatc taccgcttta atccgctctg ccggcaaaac ggcaaacctg tccaagaaaa
    37381 accgcatcgc ctcacgcgaa atgatatagc cagccgtccc acaatgttcg ctctccagca
    37441 aaggaaatga ccggttttca taattcagga ctttatccgg tctgacaata actttcgcaa
    37501 acatcgtttc caagcggacg acaaaggcgg aatccttatc aaaacgctct tccaaccaag
    37561 tatcttcggc aaggaacttt tctgcgtctt tgccaaacag gacatcatcc tcaaatacgg
    37621 caacatacgg tacgccttcg tccaatgcct gtttccacaa tacggcgtgg ctcataaagc
    37681 aggctttttc cactccgctc aaataggggt gcgccgacaa gccggggacg agttccgcca
    37741 tcgcccgttc cagcctttca gacggcatca gtgcgtcgaa aaactggaac gggatgccgc
    37801 gactgccgaa ggtatcggca atgtgcgccc tgcgctctgc ggcggaagcc aagctgataa
    37861 cgtggttttg catgatttat cctgttcggt ttcaatagct gcggtatttc caagggcgta
    37921 acattttttt caggattttc ctatattgcg gtgcggcaaa cagcttcctc atcacgcggt
    37981 ctgtcaagaa atccagcaaa tcggtcaaag aatacttttc caacgacaag aaatattcgt
    38041 acaggaacag gcggaggcat ccgatatcct gtccggacaa ggctttttcg tacaacgcat
    38101 atgccgttga tttcaaaagc ccgtaattca ggcagtccgc cccgacggct atgcctgccg
    38161 ccttccaata ccccgccctg atttcttctt tgattttcca cgccgtcctg cgctgttgca
    38221 ggttgtattt ggaagaagtc tggtcttgat ggaagcggta tttgaccaag gcttcgggat
    38281 aataagccag cctgcccagt ttgccggctt cgtaccaaaa cttatagtct tcggcgtgga
    38341 tataggctgg atcgaaccgc aaaccgccgt caatgacgct gcgcctcata atcatcgtgt
    38401 tgttgtgtat ggggttgccg aaagggaaaa cggcgacaat gtcttcatgc cgggtcggtt
    38461 tgtcccaaat tgcgccgttt cgggcaatgg cggcaagcac gcttttattg ttttcttccg
    38521 acaaaacttc caaccacgcg cccatcgcaa tgatgctgcg gtctttttcc atctcgccca
    38581 cgattttctc aatccagccg ggggaggcaa tatcgtcggc atcggtgcgc gcaatatatt
    38641 cccccccccc cgactttgcc aattcgtcca gcccgatgtt taaagaggcg ataaagccca
    38701 aattgcgggg attggaaatt atcctgatcc tgccgtcctg ttcttggaaa cgccgggcaa
    38761 tggcgggcgt gccgtccgtc gagccgtcat cgacaatcaa aatatccaag ttgcgccaag
    38821 tctgccccac tacggcggac agcgattggg caaaatattt ttctacgttg taggcgcaaa
    38881 tcaatacgct gactaaaggc tgcaatccgt ttctcctttc cgatacgtcc gatgccgtct
    38941 gaaggcttca gacggcctgc cccgtcaata aatcttgcgt aagaatctgg cagacagctt
    39001 tttgcgccat ctttgaagca tacgctttgt cggcgggacg gcaagtttgc cgcgccattc
    39061 ttcgggaacg gtcgtcaggc tgccggccaa ctctgtgaaa cgttccgcac cccaaacggt
    39121 gcagtccctg tgccacggct ttgccgagcc gcaataatgg ctgacggcga cgggcatcgc
    39181 cgtattggta cggtcgaggt aaagcgggtc ggtatggcgg gacgcaaacc cgttcgccat
    39241 aaaggcataa ttggtcggca taaagttgaa acggctgttc gcataacaca ccccgccttt
    39301 aaacagcccg ttcaaaatgt cctgatcctg atattgcatc acgtccttgt attgttccac
    39361 ccattcgcag gacattttga aaatatcgtg ccgccgccac tttttcaggt tgatcagcaa
    39421 tacgccggca ttgaaataat attctccgtc cgccataccg attttttgtt tgtatccttc
    39481 ctgcctttcg acaaacaaat cgatgcacgc gccgacccag ttaccgccca aatcggtatc
    39541 ccataagggc ttcaggccgt ccctgaccaa tacgtccgta tccagataca ggactttgtc
    39601 gcaatcggca atgtattcgc ccaatttcag gcgggcataa gtcgtaatgg aaatgtgcct
    39661 gatgtttaag gggaagccgg cgaaatcttc gaggc
//
LOCUS       DAWTCI010000008        38654 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWTCI010000008 DAWTCI010000000
VERSION     DAWTCI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38654
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.008"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..2081)
                     /locus_tag="WM481_000464"
     CDS             complement(<1..2081)
                     /locus_tag="WM481_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9079853.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGRGGQAQAQTYKYAIVMN
                     AQNQPEVKSNVPYSIKDKDRKRVFDYTSQKSSLGRTNNFISFNNNDEVVARKNGTAVF
                     GTATYLPPYGKVSGFDTDSLKGRANAVDWIRTTRPGLAGYAYTDVICRDAGQCPQLVY
                     ETKFAFGQQGLQRNGNKLDIYEDKSRDNSPIYKLKDYPWLGVSFNLGSENTVKNGRSS
                     GRLISSFSENNNNQTIVSTTRDRPISLGDRQREHTAVAYYLNAKLHLLDKKRIKDIAQ
                     GKTVDLGTLRPRVETTGRNWLSYWATWKIEDKGNITVRLGLPEVKAGRCINKPNPNPK
                     SALSPALTAPALWFGPVQNGKVQMYSASVSTYPDSSSSRIFLQELKTQTEPGKPGRYS
                     LKSLNDGEIKSRQPSFNGRQTIIRLDDGVHLIKLNGSKDEVAAFVNLNGNNTGKNDTF
                     GIVKEANVNLEADEWKKVLLPWTVRGPDNDNKFKSINQKPEKYSQRYRIRDNNGNRDL
                     GDIVNSPIVAVGGYLATSANDGMVHIFKKTGTDQRGYELKLSYIPGTMPRKDIESNDS
                     TLAKELRAFAEKGYVGDRYGVDGGFVLRQVNLNGQDRVFMFGAMGLGGRGAYALDLSK
                     INGNYPAAAPLFDVKDGDNNGKNRVEVKLGYTVGTPQIGKTQNGTYSAFLASGYAAKK
                     IDDSTNKTALYV"
     gene            2796..3188
                     /locus_tag="WM481_000465"
     CDS             2796..3188
                     /locus_tag="WM481_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079854.1"
                     /translation="MQKKRHSRRHLFIPTSSKHISGLNIHSIPVLKGTGSHSVKKSGM
                     SSAQQANYPQNLFPTKNNPQVCTKGNPPYRHPFKTAIQMILKIKKSYPQKIPHINIII
                     VLLFKPYILFITLQGNGTAKRKGIFSKN"
     gene            3268..4716
                     /gene="gnd"
                     /locus_tag="WM481_000466"
     CDS             3268..4716
                     /gene="gnd"
                     /locus_tag="WM481_000466"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFA9079855.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKETGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFVEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLAEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGRLFARTATPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            4779..6050
                     /gene="waaA"
                     /locus_tag="WM481_000467"
     CDS             4779..6050
                     /gene="waaA"
                     /locus_tag="WM481_000467"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFA9079856.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPHP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRRRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEDMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLAVVPRHPEHFQTTFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWVGDSMGELYAYYLCADVAFVGGSLVGSGCQNII
                     EPLSCGVTTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSGEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            6091..6540
                     /locus_tag="WM481_000468"
     CDS             6091..6540
                     /locus_tag="WM481_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFA9079857.1"
                     /translation="MSIYAVAHIIHLYCATAFVGGVFFEVLVLSVLHTGRVSREARRE
                     VEKAMSYRAVRVMPFAVGLLFASGIVMAANRYLPISGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            6573..7547
                     /locus_tag="WM481_000469"
     CDS             6573..7547
                     /locus_tag="WM481_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9079858.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGIKET
                     LAWSGLWVAVSCLFAGWLYFELAGNPGYGAAAAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTAFHGEKFFTVENGKKIATPLFLVLIMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            7616..7691
                     /locus_tag="WM481_000470"
     tRNA            7616..7691
                     /locus_tag="WM481_000470"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7649..7651,aa:Lys,seq:ttt)
     gene            7912..9165
                     /gene="murA"
                     /locus_tag="WM481_000471"
     CDS             7912..9165
                     /gene="murA"
                     /locus_tag="WM481_000471"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFA9079859.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVATTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVNQHLKGLEAMGAEIAIEHGYVKAKGKLKGARVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     GELYGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVERIY
                     HLDRGYENIEKKLGSVGANIERVSG"
     gene            9246..10439
                     /locus_tag="WM481_000472"
     CDS             9246..10439
                     /locus_tag="WM481_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFA9079860.1"
                     /translation="MAFLKLTEQNVRGKTVLIRADMNVPFKGGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYAALCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTEQIGYISTGGGAFLEFLEGKELPAVAALEKTRR
                     VNGLI"
     gene            complement(10491..10739)
                     /locus_tag="WM481_000473"
     CDS             complement(10491..10739)
                     /locus_tag="WM481_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9079861.1"
                     /translation="MLTPEQVKALIAGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(10832..11749)
                     /gene="ftsX"
                     /locus_tag="WM481_000474"
     CDS             complement(10832..11749)
                     /gene="ftsX"
                     /locus_tag="WM481_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HFA9079862.1"
                     /translation="MSIIHYFSLHVESARSALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLTRDKRLDNIRFIGKEDG
                     LAELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSIFSAAVSLGLCGWLLSAVHPLVDAIFKPYGLNIGWRFFYVGELG
                     LVFGFVIALGVFGAWLATTQHLLCFKAKK"
     gene            complement(11746..12396)
                     /locus_tag="WM481_000475"
     CDS             complement(11746..12396)
                     /locus_tag="WM481_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFA9079863.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSMGKVLFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILNDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(12591..13073)
                     /locus_tag="WM481_000476"
     CDS             complement(12591..13073)
                     /locus_tag="WM481_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9079864.1"
                     /translation="MKRLNLAAIALAATFAAHTASADELAGWKDNTPQNLQSLKAPVR
                     IANLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            13197..13550
                     /gene="arsC"
                     /locus_tag="WM481_000477"
     CDS             13197..13550
                     /gene="arsC"
                     /locus_tag="WM481_000477"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFA9079865.1"
                     /translation="MSEIKIFHNPRCSKSRAAVSLLEERGIAAEAVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            13547..14203
                     /locus_tag="WM481_000478"
     CDS             13547..14203
                     /locus_tag="WM481_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFA9079866.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            14319..15713
                     /locus_tag="WM481_000479"
     CDS             14319..15713
                     /locus_tag="WM481_000479"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFA9079867.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFDRYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCAVVDDYDMGVTHIIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTTEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPDGKLTELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAKAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALISKEEVLKRIRA"
     gene            15734..16129
                     /locus_tag="WM481_000480"
     CDS             15734..16129
                     /locus_tag="WM481_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079868.1"
                     /translation="MPSDVGMRLQTAFRRKLNMKKILYFLMFVFSTSVWAGGAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEEMRQIGVRLDHAWNAVYGAGERKRRGN"
     gene            16133..16624
                     /locus_tag="WM481_000481"
     CDS             16133..16624
                     /locus_tag="WM481_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9079869.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSAAVKRHDMENFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDV
                     DKV"
     gene            complement(16900..19494)
                     /gene="mutS"
                     /locus_tag="WM481_000482"
     CDS             complement(16900..19494)
                     /gene="mutS"
                     /locus_tag="WM481_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFA9079870.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYELFLDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTADK
                     LNDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDSKEHAVSIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKTPTLFSILDGCATHMGSRLLALWLHHPLRNRAHIRARQEAVTALESQYEPL
                     QCHLKSIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSATGSSLLETLKAVFP
                     ETLPVAETLKAAVMPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQDQALALEKQLFDGVLKNLRTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVVHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPVDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSI
                     VLMDEVGRGTSTFDGLALAHAIAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKAAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVDCFVDKAEEKHFEGILAAALENLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            19925..20929
                     /gene="gap"
                     /locus_tag="WM481_000483"
     CDS             19925..20929
                     /gene="gap"
                     /locus_tag="WM481_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9079871.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVAAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(21081..21761)
                     /gene="tsaA"
                     /locus_tag="WM481_000484"
     CDS             complement(21081..21761)
                     /gene="tsaA"
                     /locus_tag="WM481_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFA9079872.1"
                     /translation="MTYTITPIGAARSPYKQKFGIARQPGLVSAAEACIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGSDLLDGTPIVDIKPYIPFIESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     INLSPTPL"
     gene            22221..22856
                     /locus_tag="WM481_000485"
     CDS             22221..22856
                     /locus_tag="WM481_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFA9079873.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMAAKWLVDNPVDMLKPTGFSDADLTWVAHAMVGTRGYELLDGLPSVKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTNMVIVGGLATDYCVKTTVLQL
                     LKGGRWQVIVNEAACRGIAPDTIEAAWQEMRSSGAIILKNAEKIKKYINNQ"
     gene            22893..23861
                     /gene="waaC"
                     /locus_tag="WM481_000486"
     CDS             22893..23861
                     /gene="waaC"
                     /locus_tag="WM481_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFA9079874.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCSDVELHWLCEAGFAGI
                     ARLHPFVKKVHVMKWRQWRKHLFQAETWREMGRLKQALLQEAFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKHSAREGVAALAYDKKYVVPKGRNAVWRNRDLFAQVFGYAMPETQV
                     FGLSVPEASRLKNLAQPYYAALHATSRDSKLWPMENWRELLQKLNQKQQCNVYLPWGN
                     EAEKVRAEQIADGLPFTIVCAKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSAIAKNVGNIGQIPTADLVYQTLMDCVSADKG"
     gene            24087..24836
                     /locus_tag="WM481_000487"
     CDS             24087..24836
                     /locus_tag="WM481_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFA9079875.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVHLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHIETDVKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFVSKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLDSADLATDISPRLKTV
                     KFAEPKARQTGVKVASVAELVEKLKNEAKVI"
     gene            24847..25782
                     /locus_tag="WM481_000488"
     CDS             24847..25782
                     /locus_tag="WM481_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFA9079876.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVAAAAKLGKVDLLVVGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRVTAFDAVAAQGGN
                     AEVINVKTTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSVEKFSEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLIELLKN"
     gene            25845..26456
                     /locus_tag="WM481_000489"
     CDS             25845..26456
                     /locus_tag="WM481_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFA9079877.1"
                     /translation="MITTYKTITSPTQAEFKDKGSRFIAFAYPIRTLADVKKYLNLLK
                     EEHYKARHWCYAYRLGVNGMQFRGNDDGEPPGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGALLGVPGLIHAYKEATAQALAIAEVVEKNIEKTVWLKCEYPFLNEAICIAKQYQAD
                     ILEQDLQLDCRLTVSLSLVNYEACVAAWKNTRQVEVNTEKPFE"
     gene            complement(26508..26846)
                     /locus_tag="WM481_000490"
     CDS             complement(26508..26846)
                     /locus_tag="WM481_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFA9079878.1"
                     /translation="MKKEQANTQKRIDEIQSKYREWCHLLLQLEENIRRWKYAVALIR
                     DMDNFYTHEYQVRHQTIEDGVELNLQTEGEYSIMSEDALWNAPGEFHQLAWLYLCSSV
                     DTLDRYTQEN"
     gene            26983..28254
                     /gene="purD"
                     /locus_tag="WM481_000491"
     CDS             26983..28254
                     /gene="purD"
                     /locus_tag="WM481_000491"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFA9079879.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIESKLQ
                     NIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLVAGKGVIVAMTLD
                     EAHAAIDDMLLGNKMGNAGERVVIEDFLQGEEASFIVMVDGNHVLPMATSRDHKRLLD
                     GDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDDVNRIGKVFHAGTTVNEKGDVLTNGGRILCVVGLGDDVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            28288..28890
                     /locus_tag="WM481_000492"
     CDS             28288..28890
                     /locus_tag="WM481_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFA9079880.1"
                     /translation="MTDALQTAFYIMLFSRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQLEQLQPLLQMPFENIQTMLRNEWPA
                     PKTFLLLAKSGVLPALRGKRRSKLAVRVPDHTGARRLCQALGMPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            28905..29420
                     /locus_tag="WM481_000493"
     CDS             28905..29420
                     /locus_tag="WM481_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079881.1"
                     /translation="MKTFLHTQRKNFLDGISLHDCYRTAIKVEGRNVCFDFEDGFVVL
                     DNNPNNQAGKHLKTDFSRVVLTHENRNAEDDYLVDIFEDIVFLGKRLFTVRKFLDFSE
                     LLEMINAQGYFLEFLYLYECIGVKTSDYFLEAVLVTGRSRECKKCFIKIMRASSFVYQ
                     WNNLRLNSEIV"
     gene            complement(29572..30012)
                     /locus_tag="WM481_000494"
     CDS             complement(29572..30012)
                     /locus_tag="WM481_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079882.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(30244..30423)
                     /locus_tag="WM481_000495"
     CDS             complement(30244..30423)
                     /locus_tag="WM481_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFA9079883.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYMHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            complement(30429..31016)
                     /locus_tag="WM481_000496"
     CDS             complement(30429..31016)
                     /locus_tag="WM481_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFA9079884.1"
                     /translation="MPLPDLTDAELMESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSNEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            complement(31038..31784)
                     /locus_tag="WM481_000497"
     CDS             complement(31038..31784)
                     /locus_tag="WM481_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFA9079885.1"
                     /translation="MQPETSAQYQHRFSQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSKEWSRLKEGFVRDARAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTHDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPKADNWKNILLGKWSG
                     WIEQRIIIPSLSAISENMEGNSPSQNHLSA"
     gene            complement(31774..32616)
                     /locus_tag="WM481_000498"
     CDS             complement(31774..32616)
                     /locus_tag="WM481_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HFA9079886.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGRAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMCRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAGRVCYIHIAGHDAETPELLIDT
                     HGAAVLPTVWDLLELAYTKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            complement(32734..33042)
                     /locus_tag="WM481_000499"
     CDS             complement(32734..33042)
                     /locus_tag="WM481_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079887.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAAHKPASNATGVQKSAQGSCGA
                     SKSAEGSCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            complement(33116..33565)
                     /locus_tag="WM481_000500"
     CDS             complement(33116..33565)
                     /locus_tag="WM481_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9079888.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNFAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKLFCPKCRLKQD"
     gene            33905..34798
                     /locus_tag="WM481_000501"
     CDS             33905..34798
                     /locus_tag="WM481_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFA9079889.1"
                     /translation="MVTFSKIRSFLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVA
                     LALGGGASKGFAHIGIVKVLKENGIPVKVVTGTSAGSIVGSLLASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHKYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNVGQGFFSYLDQTLNVMSVSVLQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            34901..36004
                     /gene="prfB"
                     /locus_tag="WM481_000502"
     CDS             34901..36004
                     /gene="prfB"
                     /locus_tag="WM481_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFA9079890.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKESKILEGIVLTLDNIASGIEDNRMLIEMAVEENDEEGFAAVK
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            36083..36442
                     /gene="rnpB"
                     /locus_tag="WM481_000503"
     ncRNA           36083..36442
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WM481_000503"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(36769..38226)
                     /locus_tag="WM481_000504"
     CDS             complement(36769..38226)
                     /locus_tag="WM481_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9079891.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISSGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT         9254 a         8972 c        10814 g         9614 t
ORIGIN      
        1 acatacagcg cggttttatt tgttgagtcg tcaatttttt tagccgcata accggaggcg
       61 aggaaggcgg agtatgtgcc gttttgggtt ttgccgattt gcggcgtgcc gacggtgtag
      121 cctaatttca cttccacgcg atttttgccg ttattatcgc catctttgac atcaaacagg
      181 ggagcggcgg ccggataatt tccgttgatt ttgcttaaat ccaaggcata cgcgcctctg
      241 ccgccaaggc ccattgcgcc gaacataaac acgcggtctt gtccgtttaa gttaacttga
      301 cgcaagacaa agccgccgtc cacgccgtag cggtcgccca catagccttt ttcggcaaag
      361 gcgcgcagct ctttggcgag tgtagaatcg ttgctttcaa tatccttgcg cggcatcgta
      421 cccgggatgt agctgagctt cagttcgtag cctcgttggt ctgtgccggt ttttttgaag
      481 atatgcacca tcccgtcgtt ggcagaagtt gccagatacc cgccgaccgc gacaatcggg
      541 ctgttgacga tgtcgcccaa atcgcgattg ccgttgttgt cgcggatgcg gtatctttgg
      601 ctgtattttt ctggtttttg gttaattgat ttaaatttat tgtcattatc gggaccccga
      661 accgtccaag gcagcagcac ttttttccac tcgtcagctt caagattgac gttcgcttcc
      721 ttaacaatgc cgaaagtgtc gtttttgccg gtgttgtttc catttaaatt gacaaaagcg
      781 gcgacctcat ccttgcttcc attcagtttg atcaaatgta cgccgtcatc caatcggatg
      841 attgtttgcc gcccgttgaa actcggctgt cgacttttaa tctcaccatc attcaaagat
      901 ttgagggaat agcggccggg tttgccgggt tcggtttgag ttttcagctc ttggaggaag
      961 atgcggctgc tcgaactgtc ggggtaggta gaaaccgaag cggaatacat ctgcaccttg
     1021 ccattttgca cagggccgaa ccacagcgcg ggggcggtca gtgccggcga aagggctgat
     1081 ttgggattgg ggttcggttt gttgatgcag cggcctgctt tgacttccgg caggccgagg
     1141 cggactgtaa tgttcccttt atcttcaatc ttccacgtag cccaataact tagccagttt
     1201 cgtcctgtcg tctcgacgcg cggtctcaag gtgcccaaat ccactgtttt gccttgggcg
     1261 atatctttaa tccttttttt gtccagcagg tgcagcttgg cgttcagata ataggccacg
     1321 gcggtatgtt cgcgctgccg gtcgccaagg gaaatagggc ggtctcgtgt cgtagagacg
     1381 atggtttgat tattattgtt ttcactaaaa gaagatatca atctgccgga tgatctgcca
     1441 tttttgacgg tattctcgct gcccaaattg aaagatacgc ccaaccatgg ataatccttc
     1501 aatttgtaaa tgggcgaatt gtcgcggctt ttgtcttcgt atatatccag cttgttgcca
     1561 tttctttgca acccttgttg accgaaggca aatttggtct catagacaag ttgggggcat
     1621 tgccctgcgt ctctgcatat gacgtcggtg taggcgtagc ctgccagccc gggccgggtg
     1681 gtacgaatcc aatcaacggc attggcgcgc cctttcagac tatcggtatc aaaaccggaa
     1741 accttgccgt agggcggcag gtaggtggct gtgccaaaaa cggcagtacc gttttttcga
     1801 gcaacaactt catcgttatt gttgaatgag ataaaattgt ttgtcctgcc taagctgctt
     1861 ttctggctcg tatagtcaaa tacccgcttc ctgtctttgt cctttattga atatggcaca
     1921 ttcgacttta cctcgggctg gttttgcgcg ttcatcacaa tagcgtattt atacgtttgc
     1981 gcctgcgcct gccccccccg cccgccggta tgggaaaaca tcaagatggc ggcataaagc
     2041 gcggtatggc ggaaaacctg ccgtttcaaa gttttattca tattttattc cttaagtttg
     2101 ccttcgcggg acggcgcgcc gcgaacgccg gggttcggta aaccgcccga ttccgcgccc
     2161 gccgaattgc tgattgaaaa gcttacctcc ccattttaac tttgcgcact tatcacggta
     2221 aagcgttttt tagcaaaccg ccgcagatgc ccgaccggtc cggattcccg cctgcgcggg
     2281 aatgacgaat ccatccatac agaaacctgc accgcgtcat tcccgcgaac ctacatcccg
     2341 tcattcccac gaaagtggga atccagaatc ccggactttc agataatctt tgaatattgc
     2401 tgttgtccca cgttctagat tcccgcctgc gcgggaatga cggcatcggt ttgacggtat
     2461 ttaattgaat tgcggaaatt gacggattca gtgagattgg cgggatgaag cctaccctat
     2521 agcccgcctt ttatagcgga ttaacaaaaa ccggtacggc gttgccccgc cccggctcaa
     2581 agggaacggt tccctgaagg cgcccaagca ccgggcgaac cggttccgta ccatttgtac
     2641 tgcccgcggc ccgccgcctt gtcctgatct ttgttaatcc gctatgctta tacacaacca
     2701 gccaaaagcc atcttattga aatatcataa gatttactca aaaatcttat tttctccacc
     2761 acttatgcac aaactcggat acacactctg ccaatatgca gaaaaaaaga cattcgcgcc
     2821 gacacctttt catccccaca agttccaaac acatttccgg cttaaatatc cacagcattc
     2881 ccgttctcaa aggcaccggc agccattcgg taaagaaaag cgggatgtcg tccgcacaac
     2941 aagcaaacta tccacaaaac ctattcccca cgaaaaacaa tccacaggta tgcacaaaag
     3001 ggaatcctcc ttaccgacac ccttttaaaa cggcaattca gatgatttta aaaataaaaa
     3061 aaagttaccc acagaaaatt ccccatatca atataatcat cgttttatta tttaaacctt
     3121 atattttatt tataaccctt cagggaaacg gcacggcaaa gcgtaaaggc atcttttcaa
     3181 aaaattaaaa agttaaggat tgttaaacaa gtgtaaatca ggtagagttg cagccgataa
     3241 attaaatcta taacggagct tcctgcaatg aaaggcgata ttggcgtaat cggtttggcg
     3301 gttatgggtc aaaacctgat tttgaatatg aacgattgcg gatttaaggt tgtcgcctac
     3361 aaccggacaa tcggtaaagt ggacgaattt ttaaacggtg cggcaaaaga gacgggcatt
     3421 atcggcgcgt attccctgca agacttggtc gataagctgg caaaaccgag aaaaatcatg
     3481 atgatggttc gcgcaggttc ggtggttgac gactttgtcg aacaactgct tccgctgttg
     3541 gaagaaggcg acatcattat cgacggcggc aatgccaatt accccgacac gacacggcgg
     3601 acacattacc ttgccggaaa agggattttg tttgtcggcg cgggcgtatc cggcggcgaa
     3661 gaaggcgcgc ggcgcgggcc gtccatcatg ccgggcgggg ataagcgcgc ttgggaagcg
     3721 gtcaagccga ttttccaggc gattgccgcc aaaacgccgc agggcgaacc gtgttgcgac
     3781 tgggtcggca aggatggcgc gggacatttt gtcaaaatgg tgcacaacgg catcgaatac
     3841 ggcgatatgc agctgatttg cgaagcgtac cagtttatga aagacggttt ggggctgtcc
     3901 tacgacgaaa tgcaccgcgt gtttgccgag tggaacaaaa ccgagctgga ttcttatctg
     3961 attgaaatca cagcggcaat tttgggctat aaggacgaag gcggcgaacc gctggccgag
     4021 aaaatcctcg atacggcagg gcaaaaaggc acgggcaaat ggacgggcat caatgccttg
     4081 gatttgggca ttccgctgac gctgatttcc gaagccgtat tcgcccgctg tgtttcgtcg
     4141 ttcaaagaac agcgcgtgca gaccggcagg ctgttcgccc gaaccgccac gcccgtcgaa
     4201 ggcggcaaac aagaatgggt cgaggcgttg agacaggctt tgcttgcctc caaaatcatt
     4261 tcctacgcac aaggctttat gttgatccgc gaagcgggcg aaagttacgg ctgggatttg
     4321 gactacggca acactgcgct gctgtggcgc gaaggctgca tcatccgcag cgcgtttttg
     4381 ggcaatatcc gcgacgcgta tgaggccaat cccgatttgg tgttcttggg tgcggatccg
     4441 tatttcaaaa atattttgga aaactgcctg ccggcatggc gcaaggtggt tgccaaggcg
     4501 gtcgaatgcg gcatccctat gccctgcatg gcttcggcga ttacgttctt ggacggctat
     4561 acgaccgaac gcctgcctgc caacctcttg caggcacagc gcgactactt cggcgcgcac
     4621 acttacgagc gcaccgacaa accgcgcggc gaatttttcc ataccaactg gacgggcaag
     4681 ggcggagata ccgcttcgac aacctacgat atttgattcc gttgccgaaa tgccgtccga
     4741 accgttcgga cggcatttgt tttgcaggaa atagaaagat gttccaatgg ctttatgatg
     4801 tattgtggct gcttgcgccg atatggatac ggcgttattt ggacaaacgc tccggaagtg
     4861 ccccggcata ccgggcgcat agggacgagc gtttcggcaa gccgcatccg aatcccgtta
     4921 ccggcgcggt ttggatacac gccgtttcgg tcggagaaac gcgtgccgcc cagcccttga
     4981 tacgcgagtt gcggcggcgt tttcccgatg cgccgctgct gatgacgcag atgaccccga
     5041 cgggacggga aaccgcgcaa gttctgtttc ccgatgcgca atgccgctat cttccgtatg
     5101 acaaaaaaac gtgggtacgg cagtttttgc gcgaacaccg cccgatgttc ggcattttga
     5161 tggaaaccga aatctggccc aatctgatga aggaatgccg gcgagcgggt gtgccgctgt
     5221 ttttggcgaa tgcgcggctg tcggaaaaat cgctgaacgg ttatctgaaa gtccgccgcc
     5281 tgatccgtcc cgcagccgct tcgctgacgg gctgtctggc gcagacggag gcggatgcgg
     5341 cgcggttggc gaaattgggc gcggcatccg tgcaggtgtg cggcaatacc aaatacgacc
     5401 tgatgccgtc tgaagacatg aaaacgctgg cggggcagtt tgaaaaacgc atcggaggcc
     5461 ggccggttgc cgtgtgcggc agcacgcgcg tttatcgggg cgaagacgag gcggaaaaac
     5521 tgctggcggc gtggcaacaa tatcgcggcg atgccctgct ggccgtcgtg ccgcgccatc
     5581 ccgagcattt tcagacgaca tttgaaacgg cgaaacgctt cgggtttaag gttcagcggc
     5641 gcagcgacgg tttgccggtc gaacccgata cgcaggtgtg ggtaggcgac agtatgggcg
     5701 agctgtatgc gtattacctg tgcgccgatg tcgcttttgt cggcggcagt ctggtcggtt
     5761 cgggctgtca gaacatcatc gaaccgcttt cctgcggcgt tacgacgata ttcggctttt
     5821 cgacctacaa tttttccgaa gcctgccggc acgccttggc atcgggtgcg gcggttcaag
     5881 tcgaatcggc ggacgcgtgg cgtgaagccg ttgaaaaaaa cttatctggc gaggggggcg
     5941 gaatgcagat gcaggcgcgc gtggacggct ttatcgcaca acatcgcgga gcgggcgcga
     6001 gaatcgccga ggcggtgcgg gaagcggtat gcggacatcg ggggcgatag tgatacaatc
     6061 cgtatccgag ttttccggtt ggagcatcgt atgagtattt atgccgtcgc gcacatcatc
     6121 cacctgtatt gcgccaccgc ctttgtcggc ggcgtgtttt ttgaagtgct ggttttgtcc
     6181 gtcctgcata cgggacgggt gtcgcgcgag gcgcggcgcg aagtggaaaa ggcaatgtct
     6241 taccgcgccg tcagggtgat gccgtttgcg gtcggactgc tgttcgccag cggcatcgtg
     6301 atggcggcaa accgctacct tcctatatcg ggcgaaccgt ttgccacttc cttcggtacg
     6361 atgctgacgc tgaaaatcct gttggcgttc agcgtgttgg cgcacttcgc catcgccgtc
     6421 gtcaaaatgg cgcgttccac gctgacggtc ggctggtcga aatacataca cgccgtcgtc
     6481 tttacccata tgctgctgat tgtctttttg gcaaaagcga tgttttatat cagctggtaa
     6541 cacaactaca tttcaaatca ggagattgaa ccatgacaga atatcccggc atcgggtcgc
     6601 cgttgtttta cggcgttttt tttgcggcag tgctggtcat gattgccttg gatatgtttt
     6661 cgctgaagaa aaacggcagc cataaagtcg gcatcaaaga aaccttggca tggagcggct
     6721 tgtgggttgc cgtatcctgc ctgttcgcag gctggctgta tttcgaactc gccggcaatc
     6781 ccggctacgg cgcggcagcc gcaaaagaaa aagtattgga attctttacc ggctacattt
     6841 tggaaaaatc gttggcagtc gacaatattt tcgtgttcct gatgatattc ggctacttca
     6901 aagtcgcacc gcagtttcaa caccgcgtgc tgctgtacgg cgtattgggc gcattggtat
     6961 tgcgcaccgt catgattttc gtcggcgcgg cactggttcg gcagttcgag tggattctgt
     7021 atctgttcgg cgcgttcctg ctctataccg gcatacacat gatgaagccc gaaggagatg
     7081 aggaagagga tttggcaaac agcaggctgc tgaatgccgt caagaaagtc gttccggtcg
     7141 gcacggcgtt tcacggcgag aaatttttta ccgtcgagaa tggcaaaaaa atcgccacgc
     7201 cgctgttttt ggtgctgatt atgattgaat tgagcgatgt cgtttttgcc gtggacagta
     7261 tccccgccgt ctttgccgtt accaccgatc cgtttatcgt gctgacttcc aatattttcg
     7321 ctattttggg tttgcgggcg atgtatttcc tgctggcgga tgtggcggaa cgctttatct
     7381 tcctgaaata cggcttggca ttcgtgttgg gttttatcgg tgtgaaaatg ctggtgatgc
     7441 attgggtaca catcccgatt tccgtttcgc tgtcggtcgt gttcggcgcg ttgggtgcat
     7501 cgatactgac ctcgttaatt tatacgaaaa aacagcctga taaataaaac gccgtctgaa
     7561 cgccgacaaa atggttgaca gcatcaggct tgatgtataa actttcaaat ttttcgggtc
     7621 gttagctcag tcggtagagc agcggacttt taatccgttg gtcgagcgtt cgaatcgctc
     7681 acgacccacc aggtaaagca aaaaagccga gttttcaaaa ctcggctttt ttttgttttc
     7741 cgacaatcct tgttcggaaa gcgcggcatt tcgtcaaatt ctttggtgcg gcttccgtat
     7801 ttttcccgaa aatcccttat aattccgcct ctgcaacctt catcgggtga tgctttatgc
     7861 attgtcccga gtattgaaat attagcaagt acccaccagg agggaacaaa cgtggacaaa
     7921 ctgaaaatct ccgcaaacgg cccgctcaac ggggaaataa cggtctcggg cgcgaaaaac
     7981 gcggcattgc cgctgatgtg cgcgggtttg ctgacgtcgg gtacgttgcg cctgaaaaac
     8041 gtccctatgc tggcagatgt cgcaaccacg caaaagctgc ttcagggcat gggcgcgcgc
     8101 gttctgaccg acaatatcag cgaatttgaa atcaacggcg gtacggtcaa caatacctgc
     8161 gccccttacg aattggttcg gacgatgcgc gcttcgattt tggtgcttgg cccgacgctg
     8221 gcgcgtttcg gcgaggcgca agtcagcctg ccgggcggct gcgccatcgg ttcgcgcccc
     8281 gtcaatcagc atttgaaagg cttggaagcg atgggggcgg aaatcgccat cgaacacggt
     8341 tacgtcaaag ccaaaggcaa actcaaaggt gcgcgcgtgg cgatggatgt cgttaccgtc
     8401 ggcggtacgg aaaacctgct gatggcggcg acgctggcgg aaggtacgac ggttttggaa
     8461 aactgcgcca tcgagcctga agtggtcgat ttggcggaat gcctggtcaa aatgggcgcg
     8521 aaaatcagcg gcatcggaac atcgaccatg attgtggaag gtgcgggcga gctgtacggc
     8581 tgcgaacaca gcgtcgtccc cgaccggatt gaagcgggga cgttcctgtg tgcggtggcg
     8641 ataaccggcg gcagggtggt tttgcggaat gccgccccga aaacgatgga agtggtgttg
     8701 gacaaactgg tcgaggcagg tgcggtgatt gaagcgggcg acgattggat cgccatcgat
     8761 atgcggcagc gtccgaaggc ggtggacatc cgcacggtcg tccatcccgg cttccccacg
     8821 gatatgcagg cgcagtttat ggcattgaat gctgtggcgg aaggaagctg ccgcgtggtg
     8881 gaaacgattt ttgaaaaccg ctttatgcac gttccggagt tgaaccggat gggggcgaac
     8941 attacaaccg agggcaatac ggcatttgtg cagggtgtgg aacggctttc cggcgcggtc
     9001 gtcaaggcga cggatttgcg cgcgtccgcc agcctcgtta tcgccggttt ggcggcgcgc
     9061 ggcgaaaccg tggtcgaacg gatttaccac ttggatcgcg gttatgaaaa tattgaaaaa
     9121 aaactcggca gcgtcggtgc gaacatcgag cgcgtatccg gctgatcggg ttgcgatgcc
     9181 gtctgaaggc ttcaggcggc atcaattgtt tctgtatcgc aaacactgat tgaaaaggac
     9241 tgattatggc atttttaaaa ctgaccgaac aaaacgtgcg gggcaaaacc gtcctcatcc
     9301 gcgccgatat gaacgtgccg ttcaaaggcg gcaaaatcag cgacgacacc cgtatccgcg
     9361 cctcgctcgc gtccgtcaaa tactgcttgg acaacggcgc gtccgttatt gtgatgaccc
     9421 acttgggccg cccgaccgaa ggcgagttcc accccgaaga cgacgttgcg cccgttgccg
     9481 cgcacttggg cggcctgttg ggcaaagacg tgaaagtatt gaacgactgg cgcgaaaaca
     9541 aaccggcttt gaacgcgggc gatgtcgtga tgctgcaaaa cgtgcgtatc aacaaaggcg
     9601 agaagaaaaa cgatttagaa ctgggcaaag cctacgccgc cttgtgcgac gtgttcgtca
     9661 atgatgcgtt cggcaccgca caccgcgccc aagcttcgac cgaagccgtt gcccaagccg
     9721 cgcccgtcgc ctgcgccggc gtattgatgg cgggcgaact cgacgctttg ggcaaagcct
     9781 tgaaacagcc cgcgcgcccg atggtggcga ttgttgccgg cagcaaagtg tccaccaagc
     9841 tgaccatcct cgaatcgttg gcggacaaag tcgatcagct tatcgtcggc ggcggcatcg
     9901 ccaacacatt cctgttggcg gaaggcaaag ccatcggtaa atcgctggcg gaacatgatt
     9961 tggtggaaga atccaaaaaa attatggcga aaatggcggc caaaggcggt tctgtgccgc
    10021 tgccgaccga tgtcgtcgtt gccaaagcct ttgccgccga tgccgaagcg gtggtaaaag
    10081 acatcgccga cgttgccgaa gacgagatga ttttggacat cggcccgaaa tccgccgccg
    10141 cgcttgccga gttgcttaaa gccgccggta cggtcgtttg gaacgggccg gtcggcgtgt
    10201 tcgagttcga tcaattcgca ggcggcacaa aagcccttgc cgaagccatc gcccaaagca
    10261 aagcgttctc gattgcgggc ggcggcgaca cgctggcggc gattgccaaa ttcggcgtta
    10321 ccgaacaaat cggctacatc tccaccggcg gcggcgcgtt ccttgagttc ttggaaggca
    10381 aagagctgcc tgccgtagcc gctttggaaa aaacgcggcg agtaaacggt ttgatataaa
    10441 aaaatgccgt ctgaaacgaa atggtttcag acggcatttt tgtgtggcgg ttattgtgct
    10501 ttggcagccc attccgccgg agtggcggca accgaaatgg aaagtgcgtg cagttcgttg
    10561 ctttccagtt gggctttgag tccgtcttta atcaggcggt ggcgcgcgag gcgtgccttg
    10621 ccttcaaatt ctgatgaaac gatgacggcg aaaaaatggt gtccgtcgcc ttctacttcg
    10681 atgtgttcgc attttgccac gcctgcaatc agggccttga cttgttcggg tgtcagcata
    10741 gtggggagtc cttattgggg aaatgcgcca ttatactggc aaatcgaaat tccgtctgaa
    10801 aacggcttca gacggaattt ttgacggtgt tttatttttt ggctttgaag cagagcaggt
    10861 gttgtgtggt ggcaagccac gcgccgaata cgcccaacgc gatgacgaag ccgaacacca
    10921 gcccgagttc gccgacgtag aagaaacgcc agccgatgtt caagccgtag ggtttgaaga
    10981 tggcatcgac caaggggtgc acggcagaga gcagccaacc gcaaagccct aagctgacgg
    11041 cggcggagaa gatactctgc cacatagcct gataaaggaa ggggcggcgg ataaacgacg
    11101 cgggcgcacc caggagtttg gtgatttcga tttcttcttt gcggctgagg atttgcagtc
    11161 ggatggtgtt gtgtgcgaca aggacgaaag ccatacccag cgtcagggaa agaaaccata
    11221 agattttgcg gatgaactcg ttgatttggt acagcgtttg cacccattcg gtatccatag
    11281 acgcggattc gaccataggc agcttggtaa tgtcccggta gattgcctgc atttgggcgg
    11341 gcgtggttgc cgggtcgggg gtaacgataa agacatccgg cagggggttg ccgtcaagca
    11401 tggaaatcag attttggtcg aggttggact gtaattccgc caaaccgtct tccttgccga
    11461 tgaagcggat gttgtcgagc cgtttatcgc gcgtcagcag gctgcgtacg gtatcgctgt
    11521 cgctttgtgc ggcggcggtt tccatataga cggtgatttg cggcgactcg ttgagtttgc
    11581 ccaacacgct ttgcccgctt tggatgccca gatacataaa cagcggcagg gtcatcgcga
    11641 cggcaagcat tatgagggta agcagtgtgc cgaagggttg gcgcagaagc tgtttgagtg
    11701 cggagcgcgc ggattcgacg tgcagcgaga agtagtggat gatgctcatg cgagtcgtcc
    11761 tttcgagagg cgcaggatgc ggtgtccgta gtccgccatc agggtttcgt catgtgcggc
    11821 gacgatgacg gtggttcccg cttcgtggaa ggttttgaac aattccataa tatcgagcgc
    11881 gtaggcacgg tcgaggttgg cggagggttc gtcggcaatc agcaggccgg gctggtggac
    11941 gacggcgcgg gcgatgcaca ggcgttgttg ttcaccgccc gagagggtta cgggatcgtc
    12001 caattctcgt cctttcaagc cgactttttc gatggcgatg cgggcgcgtt cttcggcttt
    12061 gcgcggcgga tagccgataa tccgcagggg caggatgacg ttttgcagga cattgcggtc
    12121 gttgaggatt ttgtggtctt ggaacacgat gccgatgtgt tggcgcataa agccgatttg
    12181 gttgtcggac aatgtgccga ggtcctgccc gttaaacagg actttgccca tgctcggctt
    12241 ggtgatgccc gaaatcagct tgaggacggt ggatttgccc gaaccggagt gtcccgcgat
    12301 aaaaatcatc tcgcctttgt tgatttggaa gctgacgttt ttcagggctt caaaaccgcc
    12361 gggataggtt ttggaaactt gttcgaaacg gatcatagga ggtcctgtaa ggaagggcgg
    12421 catatcgttt cgggcggttg cgtccgcagg gatatgccgt ctgaacaata ggtaagacag
    12481 taccgattat aaagattccg cctgttttta gatagtggat aagtttggcg gataccgaag
    12541 gccgggcggg aaaaatgccg tctgaagcgg cttcagacgg catccggcgt ttaacggcat
    12601 tttgaatggg cgagtttgac ggcttcggtc aggcttttct cgttcacctc tcctgtaatg
    12661 gtctgcctgt atccgcattt cggggcttcg acgacggtaa agggcagtac gccgacattg
    12721 tttccgtagg atttcataaa gtttcggctg ttcgccccgg tgtaacgcca aatcgggtag
    12781 ctgaccgggg tctgcttgag aaagttgccg atattgtcgg atgtgtcgag cgcgatgccg
    12841 accatatcga cgctgccttt tttctgcgct ttgtaccatt tggacatggc gggcatctct
    12901 tttcgacacg gaccgcacca agtcgcccat aggttggcga tgcggacggg ggctttgagc
    12961 gattgcaggt tttgcggggt attgtctttc catccggcca gttcgtctgc cgaggcggta
    13021 tgtgcggcaa atgtggcggc aagggcgatt gcggcgagat tcaagcgttt catcgttttg
    13081 tcctttgtat gtcgattcgg gtattgtaaa ccgtttggta cggttggagg cggggtattg
    13141 agtaaaaccc tgcgtaagcc gataataagg ggattgtcga gaagtaggga aatgtcatgt
    13201 ctgaaatcaa aattttccac aatccgcgtt gcagcaaatc gcgtgccgcc gtgtccctat
    13261 tggaagaacg cggcattgct gccgaagcgg tcaaatattt ggatacgccg cccgacttgt
    13321 ccgaattgaa ggatattttc aacaaattgg gcttggaatc ggcgcgcggg atgatgcgcg
    13381 tgaaagatga tttgtacaag gaattgggtt tggacaaccc cgatttggac aatgacgcgc
    13441 tgctgcgtgc catcgccgat catcccgccc tgttggagcg tccgattgtt ttggcaaacg
    13501 gtaaggcggc tgtcggcaga ccgttggaaa atatcgaagc ggtattgtga acaaacggct
    13561 gttttgcagc cgcaacggct tgcgctatta cctgttgggc ggtttctgtt tgagtgtttt
    13621 tccgcttttg ctggtgtttg cctcatccgt ttgggcggtt tatcggacgg gcgggcaggt
    13681 tttgccgccg tatgtccggg cggatgccgc gctggtgttg ggcgcagccg cgtgggacaa
    13741 acgcccttcc ccggtttttc gggaacgcat caaccacgcc atcgcgcttt accaaagccg
    13801 cagggtaggc aaaatcgtgt ttacgggagg gcgaaccaaa aaaggctata tgaccgaagc
    13861 cgaggtcggg cggcggtacg cgctcaaaca gggggttccg gcgcgcaaca tcctgtttga
    13921 aaacacttcg cgcaacactt atgaaaacct gaacaacatc cgtccggttt tgcgtgccaa
    13981 cggtattgcc agcgtggtga ttgtcagcga tccctaccat ttggcgcggg cggcggaaat
    14041 ggcggaggat ttgggtgtcc gcgtttatat gtctgccacg ccgaccacgc ggtttgatgc
    14101 gggaaataag aaaaaaatct ttatgctgca agaggggtat gcgttatccc tctaccgttt
    14161 ggaaaaatgg ggcagccgct ttttgggatg gctgtctgat tgaagcggta gaaaatgccg
    14221 tctgaaactt atccgcaggc agggttcgga cggcattttt gatgcctaag cacggtaaaa
    14281 acagtaaaat aaacgctttc aaagatttcg gagccattat gaccgtcaaa acccgtttcg
    14341 cccccagccc caccggttac ctgcacatcg gcggcgttcg caccgccttg ttttcgtggg
    14401 cgtttgcccg ccatcataaa ggcgaatttt tattgcgtat cgaagacacc gacttggcac
    14461 gttccaccgc cgaatccgtc aacatcatcc ttgacggcat gaaatgggtc ggtctcgatt
    14521 acgacaacgc cgacaacgtg gtgtaccaaa cccgccgttt cgaccgttat aaagaagtca
    14581 ttgccgaact cttggcaaaa ggcgatgcct actactgcta ttgcagcaaa gaagaattgg
    14641 aagcgatgcg cgagaaagcc gaaaaagaag gcacggcgac ttacgaccgc cgctggcggc
    14701 cggaagcagg caaaaccctg cccgaaattc ctgcaggcgt gcaacccgtc gtccgcttca
    14761 aaacgccttt ggacggcgtt accaaatgga cagacttggt caaaggcgaa atctccatcc
    14821 ccaacgaagc cctagacgac ctgattattg cccgcgccga cggtacgccg acctacaact
    14881 tctgcgccgt ggtagacgac tacgacatgg gcgttaccca cattatccgc ggggacgacc
    14941 atgtgaacaa caccccgaaa caaatcaaca tcttgaaagc catcggcgcg aacctgcccg
    15001 aatacgggca tctgccgatg attctcaacg aacaaggcaa aaaaatctcc aaacgcagcg
    15061 gcgataccgt cgccattacc gatttcggcg caatgggcat tctgcccgaa gcgatgctca
    15121 actatttggc gcgtttgggc tgggcgcacg gcgacgatga gtttttcacg acggaacaat
    15181 tcatcgaatg gtttgatttg aaagacgttt ccccgtcccc aagccgtatg gatttgaaaa
    15241 agctctattg gatcaacggc gaacacatca aaatcacacc cgacggcaaa cttaccgaac
    15301 tcgtcaaacc gcgccttgcg ctgcgcgata ttcatgaaac cgaaaaacct gcgttggaag
    15361 acgtgttggc actggtcaaa gaccgcgccc aagatttgaa cgccttggca gacgaatgcc
    15421 tgtatttcta caaaaaacaa gtccctgccg aagccgatgt tgccaaacac tgggacgacg
    15481 aagccgccgc ccgtatgctg cgcttcgccg aacgcctcga agggctggaa gactggaacg
    15541 ccaaagccat tcacgacctc ttcaaacctt tctgcgacga agaaggcatc aaaatgggca
    15601 aactcggtat gcccctgcgc cttgctgtct gcggtacggc aaaaaccccg agcgtcgatg
    15661 ccgtgttggc attaatcagc aaagaggaag tgttgaaacg catccgtgct taagttatcc
    15721 acaggtgcgg gcaatgccgt ctgatgtcgg aatgcggctt cagacggcat tcagacggaa
    15781 gttgaacatg aaaaaaatat tgtattttct gatgtttgtt ttttctacaa gcgtatgggc
    15841 agggggtgcg gaagacaatt tgctcagcat ccaatccggt taccgtgctt tactgcaaaa
    15901 gcaaaacaat ctggacggaa aaatcatcgg gatgcagtcg gatttggaag atgcgcgccg
    15961 gcgtttgcag gcggctcagg cagacatcgc ccgtttggaa gcggaaattc ctgcagcaat
    16021 ggcccaaaaa gcccggcagg ctgaagagat gaggcaaatc ggagtgcgtt tggaccatgc
    16081 ttggaatgcg gtttacggcg caggggaacg aaagcgtcgg gggaattgac cgatgaattc
    16141 cctctttgtg gacaacactg tttttattac acggctgaaa gccgggcata tcggcaggtt
    16201 ggttcaggcg ttgtttgagg agtggcacgg atttgaaccg tggtcttctg tggataagat
    16261 tcgtgcctat tacggcagat gtttgaagga tgacgaattg ccgctggctt ttgcggctgt
    16321 ggatgattcc ggaaccctgt tgggttcggc tgcagtcaag cggcatgata tggaaaattt
    16381 tccacagtat gaatattggt tgggcgatgt ctttgtttta cctgaatatc gcggaaaagg
    16441 cattggcagg aggctggtcg cccactgcat aggcgcagcg cgttcgctgg ggataaagtt
    16501 cttgtatctt tatacgcctg atgtgcaaat attttatgaa tcattcggct gggcggttgt
    16561 cgggcgacat ttccataacg gggaatgggt tacggttatg cgtttggatg tggataaggt
    16621 ttaagccgaa tgggcagatg ccgtctgagc tgttcggacg gcatttttta tgtttggaaa
    16681 tttcctgtca gatatagtgg attaacaaaa accggtacgg cgttgccccg ccccggctca
    16741 aagggaacga ttccctaagg tgcccaagca ccgggcggat cggttccgta ccatttgtac
    16801 tgcctgcggc ccgccgcctt gtcctgattt ttgttaatcc gccataaagt ttatccacag
    16861 atttttccat atggtttgat gctgctccgc caacggaaat taagatacgg atttgcacaa
    16921 atctttcaga cggtacagtt ctgacaatgc ttcgcgcggg gtcaggctgt cgggatcgag
    16981 gttttccagg gctgctgcca atataccttc aaaatgtttt tcctctgctt tatccacaaa
    17041 gcagtccaca ttcggttcat ctcctttttc agacggcatg gtactgaaaa tatccagttg
    17101 gggacggttt gcggcggctt ggttttccag tccgttcaaa tgcttttggg cggctttcaa
    17161 tgcgcgcaca ggcaggccgg cgagtttggc gacggcgatg ccgtagcttt ttccggcggg
    17221 accgggttgg atttggtgca ggaacacgat gtcccgtccc tgttcgagcg cggaaaggtg
    17281 catattgacg gcggcggcgt gggcttcggg caggtaggtc agctcgaaat agtgggtggc
    17341 aaacaggctg aaggatttgt ttttttgcag caggtgttcg gcgattgcgt gcgcgagggc
    17401 gaggccgtcg aaagtggaag taccacgtcc gacttcgtcc attaaaacaa tgctttgttc
    17461 ggtggcgtga tgcaggatgt aggcggtttc gctcatttcg accatgaagg tggagcggtt
    17521 ggaggcgagg tcgtccgatg cgccgatgcg ggtgaagatt tgatcgacag gcccgattgt
    17581 ggcggcatcg gcaggcacga aacagccggt gtgtgccaat aaaacaatca gcgcgacttg
    17641 gcgcatatag gtggatttgc cgcccatatt ggggccggtg agcagcatga ggcggtgttt
    17701 gtggtcgagg tcggtgtggt tggctgtgaa gtggcgtacc tgctgttcga caacgggatg
    17761 gcggccgttt tcgatgtgga caaccggata gtcggcaaac tcgggacgga caaagttccg
    17821 ctcttttgcc aaggctgaaa atgtggacaa cacgtccagc gcggcggcgg ctttggcggc
    17881 tttttgaagc tgcggcaatg ccgtccgaag gtttttcaat acgccgtcaa agagttgttt
    17941 ttctaaggcg agggcttggt cttgagcagt cagcactttg tcttcaaagg ctttcagttc
    18001 cggcgtgatg aagcgttcgg cgtttttgag ggtttgccgg cgttggtagt cggcaggtgc
    18061 ttgttcggct tgggttttgg acaattcaat gtaaaagccg tgaacgcggt tgaactcgac
    18121 tttgagtgtg gacaaaccgg tacgttcgcg ttccttggct tccaaatcca gcaaaaattc
    18181 gtcgccatgg ttttgaatgc ggcgcaattc gtccagttcg ggatgaaaac cgtggttgat
    18241 gacattgccg tctttcagcc agacggaagg ttcgggcatc acggcggctt tgagggtttc
    18301 ggcaactggc agggtttcgg ggaaaacggc tttaagggtt tccaacaggc tgctgccggt
    18361 tgcggacaag tcgatttgtg ccaactcgaa cagactgtcg cgtaaagatg ccaaatcgcg
    18421 cgggcgggca ttgccgacgg cgatgcgggc ggcgatacgt tcgatgtcgg caatgctctt
    18481 cagatggcat tggaggggtt cgtattggct ttccagcgcg gttacggctt cttggcgcgc
    18541 tcggatgtgg gcgcggttgc gtaaagggtg gtgcagccac aatgccaaca ggcggctgcc
    18601 catgtgggtg gcgcagccgt cgagtatgga aaacagggtc ggcgtttttt tgccggagag
    18661 ggtttgcgtg atttcgagat tgcggcgcgt ggcggcatcc atgccgatat attggctgtc
    18721 ggtttcgagc gacaggccgt ccaaatgttg cggcatcagg ttttgcgtca ggcggatata
    18781 gttcaacagc gcacctgccg cgccaatcga aacggcgtgt tctttgctgt ccaaaccgaa
    18841 gccgcgcaaa tcttggcagc cgaaatattc cgtcagcagt ttttcccccg cgtcggcggc
    18901 aaactgccac gcgttcaggc gtgtaacacc cgatgccgtc tgaagttgcg gtgcgttttt
    18961 actgtccggc agcaaaattt ccgccgcctg caggcgcgcc agttcgtcgt tcaatttatc
    19021 cgcagttgtc agcttggttt tgaattcgcc gctttgcagc gatgcccacg ccaaaccgat
    19081 gtattttttg tcgggagaca cggcaacgat gcggttggtt tccttgtctt ccagtaatgc
    19141 ggaatcggtt agcgtgccgg gcgttacgat gcgcacgact ttgcgctcca ccggcccttt
    19201 gcccgcgccg acttcgccga cctgttcgca aatcgccacg cttttgccca acttgaccag
    19261 gcgcgccaga tactgttccg ccgcgtgaaa cggaacgccc gccattttaa tcggcacgcc
    19321 gtccatctgc ccgcgtgtgg tcagggtgat gtccaacaat ttcgccgctt ccaccgcgtc
    19381 atccaaaaac agctcgtaaa aatcacccat acggtaaaac actagtttgt cggtatgttg
    19441 cgcttttatg ccgaggtatt gctgcatcat tggggaaacg gcggatttgg acatggggct
    19501 gccttattat taggaaaacg gtattgtagc aaaaggtttt gtcttcgacg gaatgagact
    19561 gaccgtaagc ttgtcaataa agttgtggag acaaatagga aaacggcaaa gttatccata
    19621 agaatattat taagtcaaaa aacatccacg cttatctgtc ttatacggca agccttaaac
    19681 tcaaatttat ttaacaaaaa ggtaatgaaa atattggata tttgctactt atacacaagc
    19741 aggtcggcaa tcatcaaaat catcattaaa attttaaaat caaatattga atgcgcgttt
    19801 ttgaaatagc gaaattcacc tgcgtcaaac aaaacgcaag taattttatg taatagtgga
    19861 atgtagtttt atttcagaat acaatacagg catcggactt tatttcacaa ccgggagaca
    19921 aaatatgagc atcaaagtag cgattaacgg tttcggccgc atcggccgcc tcgcattgcg
    19981 tcaaattgaa aaagcccacg gcatcgaagt cgccgccgtc aacgacctga cccctgccga
    20041 aatgctgctg caccttttca aatacgacag cacacaaggc cgtttccaag gtactgccga
    20101 attaaaagac gatgccatcg tggtaaacgg cagagaaatc aaagttttcg ccaatccgaa
    20161 tcccgaagaa ttgccttggg gcgagttggg tgtggatgtc gtcctcgaat gtaccggttt
    20221 ctttaccaac aaaaccaaag ccgaagcaca tatccgtgcc ggcgcgcgca aagtcgtgat
    20281 ttccgcaccc ggcggcaatg atgtgaaaac cgtcgtatat ggcgtaaacc aagacatctt
    20341 ggacggtagc gaaactgtca tttccgccgc ttcctgtacc accaactgcc ttgccccgat
    20401 ggcggcagtc ctgcaaaaag agttcggcgt agtcgaaggc ctgatgacca ccatccacgc
    20461 ctacaccggc gaccaaaaca cccttgacgc gccgcaccgc aaaggcgatt tgcgccgcgc
    20521 ccgcgccgcc gcgctcaaca tcgtgcccaa cagcaccggc gccgccaaag ccatcggtct
    20581 ggttatcccc gaattgaacg gcaaactcga cggctccgcc caacgcgtcc ctgttgccac
    20641 cggctcgctg accgaattgg tttccgtcct cgaacgccct gcaaccaaag aagaaatcaa
    20701 cgccgcgatg aaagccgcct ccagcgaatc ttacggctac aacgaagatc aaatcgtttc
    20761 ttccgacgtt gtcggcatcg aatacggctc gctctttgac gcaacccaaa cccgcgtgat
    20821 gaccgtgggc ggcaaacaac tggtgaaaac cgttgcctgg tacgacaacg aaatgtccta
    20881 cacctgccaa ctcgtccgta ctttggaata cttcgcaggc aaaatctaag gcttgaacaa
    20941 acctgtggat aagtaaatgc cgtctgaaca cattgcgttc agacggcatt gtttatctgt
    21001 aacttgatat tgcggcttat ggcgaacaaa caacagccgt ttgttcaact gctatgcggc
    21061 aaaaccggat tttgcccgat ttaaagcggg gttggggaaa gattaataac cgtcgcgcgg
    21121 ttttcctcga tttgaaacct gacttcgtaa tccgcaatat tcatcacata aatccgttcg
    21181 ggaatattct gataggcggg gcgcggatct tgggcaatgc tttggctgat aaggtttttg
    21241 gtgtttgcag ataaattttc cgcgccgatg ttttcctgcc aaacgacttc caactctacg
    21301 ggtttgccgc tgacgaaacc ggatgctgca tcgggtttgg attcgataaa ggggatataa
    21361 ggcttgatgt ccacaatcgg cgtgccgtcc agcaggtctg agccgctgca atagaggcgg
    21421 acgggtttgc cggtttcgat gcgttcgagt ttaaggagag agagtccgag atggttgggg
    21481 cggtgggggc tgcgcgtggc gaacacgccc attttttgtt tgccgccgag ccgtggcggg
    21541 cgcaccattt gcgcccaacc ttcatccaat acgccgtgga agataaaact tatccacaca
    21601 taatcgaaat cttccaaccc gcgcacgctg tctgcggtaa attcgggatt cagctcgatg
    21661 caggcttctg cggcggagac caaaccgggc tggcgggcga tgccgaattt ctgtttgtag
    21721 ggcgagcggg cggcgccgat gggggtaatg gtataagtca taagattgtg gataagtttt
    21781 ttggaaaaac cttatcttat cacagccggt tttaccgctt tcgggtagta atttgttata
    21841 atacagggca tttaaatcgc gttgaaataa agtagcacag aatcctcgtg tccaagacgc
    21901 tcttaaaaag gcattggagt attgataaaa aagaagcata acggttcgtt ataacgaaga
    21961 gggaatcccg tatattacac ccaagtcagg ttgttatctt tcctttcaga tctcacctac
    22021 aggaggtgag atctgaaatt ttgggtaata aaatcggatt acagctttta gcaaggagca
    22081 ccttaggtat agctaaaatg gaggaggtgg atatgacata tcatatccac ctagatggat
    22141 tgtgctcctt aaataaagaa ggtaagagtt ttgttattcg taaaatagag aatgacgatc
    22201 ttaagtgaag aagtaatgag atgattgttt ccattgatgt tgatgcacaa aaaacgttta
    22261 cgccactgtg tcctgacgaa ctgcccgtga acgaggggca tttgattgtc gaggagttaa
    22321 atgcccaagc cgctttggcg gatttgcgtg tgatgacgaa agatgcgcat catatggcgg
    22381 cgaaatggct tgtggataac cctgttgata tgttgaagcc gacaggtttt tccgatgcgg
    22441 atttgacttg ggtggctcat gcgatggtcg gtacgcgcgg ctatgaattg ttggacggtc
    22501 tgccttccgt caaggaatac gattattgcg tttggaaagg tgtcgatccc gaattgcatc
    22561 cttacggcgc gtgttttcac gatattgagg aaaaactgag cactggattg attgagtggc
    22621 tgcgttgtca aaatacgaat atggtcattg tcggcggctt ggctacggat tattgtgtta
    22681 aaacaacggt tttgcagtta cttaaaggcg gtcgttggca ggttatcgtg aatgaagcgg
    22741 cttgtcgggg tattgcgccc gataccattg aagcagcgtg gcaggaaatg cgttcttcgg
    22801 gtgcgataat tttgaaaaac gccgaaaaaa ttaaaaaata tattaataat caataactta
    22861 taattgtttc acgtgaaaca ccctgtctac atatgaaaat tttgcttgtc cgtttgtcca
    22921 gtatgggtga tttaattcac actttgcccg caatcgaaga tttggcgcga caatgctctg
    22981 atgtagaact gcattggcta tgtgaagccg ggtttgcggg tattgcacgg ttgcatccgt
    23041 ttgtaaaaaa ggtccatgta atgaagtggc ggcaatggcg caaacatctc tttcaggctg
    23101 aaacttggcg ggagatgggt cgtctgaaac aggctttgct gcaggaagca tttgattttg
    23161 tattggacag tcagggtctg attaaaagcg cgtgtttcgc caaaatggca aaatccccta
    23221 tttatggttt ggataaacac agtgcgcgtg agggggtggc tgctttggcg tatgataaaa
    23281 agtatgtcgt accgaaaggt agaaatgccg tttggcgcaa ccgtgatctg tttgcccagg
    23341 tgtttgggta tgcaatgcct gagacgcagg tatttggttt gtccgttcct gaagcaagcc
    23401 gtctgaaaaa tttagcgcag ccgtattatg cggctttgca cgcgaccagt cgggacagta
    23461 agttatggcc tatggaaaat tggcgggagt tgctgcagaa actgaatcaa aaacaacaat
    23521 gcaatgttta tctgccttgg ggaaacgagg ctgaaaaagt gcgtgcggaa cagattgcag
    23581 acgggctgcc gtttaccata gtgtgcgcta aaatgaattt attacaggcg gcgtatctgt
    23641 tgaaacacgc ggttggaata gttggtgtag ataccggttt gctgcatttg gcaaatgcct
    23701 tggaaaaacc tgtggtcggc atttataccg ataccgatcc gattaaaaca ggcgttcaag
    23761 tttcggctat tgcaaaaaat gtgggcaata tcgggcagat tccgaccgca gatttggttt
    23821 atcaaacgct gatggattgt gtttcagcag ataaaggcta aagggtcgtc taaaactgat
    23881 atggcggcat gatattttcc aatttattag atacaggaat gtatatccgc cagactgagt
    23941 aaatacttta aaaatggtat aatcggtaaa attatccgaa agattgctaa ataaattcgt
    24001 ttttaaggaa aacggatttg attttataaa gaagaaggtt tggtttcgct ggatggtgaa
    24061 agcagcgttc tttttaggag aattcaatga aagcactggt cgcagtaaag cgcgtagtgg
    24121 actacaacgt caaagtccgt gtgaaagccg atggttcgga tgtggacatc ggcaatgtca
    24181 aaatgtcgat gaacccgttt gatgaaatcg ctgtggaaga agccgtccat ttgaaagaag
    24241 ccgggaaagt aagcgaaatt gtagcggttt ctttgggtga aaagaaatgc gaagaaacct
    24301 tgcgtaccgc tttggcaatg ggtgccgacc gtgccattca tattgaaacc gatgtgaaat
    24361 tagagccgct ggcagttgcc aaactgctga aagctgttgc ggacaaagaa aatccgcaaa
    24421 ttttcttttt gggcaaacaa gcgattgatg acgatgccaa tcaagtggcg caaatgctgg
    24481 cagctttgtt gaatgcggcg caaggtacat ttgtttcaaa agtacaaatt gaaggtgatg
    24541 aagtacagat tgtgcgcgaa attgatggcg gcgaagaaac gattgcgttg aaactgcctg
    24601 cagtcatcag cgcagatttg cgtttgaacg agccgcgctt tgtcaaactc cccaatatta
    24661 tggcggcaaa gaaaaaacct ctggaaaaac tggattctgc cgatttggct accgatattt
    24721 cgcctcgtct gaaaacggtg aaatttgccg aacctaaggc gcgtcagaca ggtgtaaaag
    24781 tagcaagcgt tgccgaattg gttgaaaaat tgaaaaacga agccaaagtg atttgaggag
    24841 gctgaaatga gcgtattgat tattgccgaa cacgacaaca aacagttgaa tcctgccact
    24901 ttgcatgctg ttgccgctgc cgccaaactg ggcaaggtcg atttattggt tgtcggaaac
    24961 ggcgcatcgt ccgtcgtgga attcgcgaag caagtagcgg gagtggaaaa agttttggtt
    25021 gcagatgctg ctcattatgc tgaaggtttg gctgaagagt tggctccgct ggttgtcaaa
    25081 ttggcggcgg attaccgcta tgttgcagca acggcaacca cattcggtaa aaaccttttg
    25141 ccccgcgtag ccgccttatt agacgtaccg caaatttctg atttgaccga gattgtagat
    25201 aacacgactt ttgtacgtcc gatttatgca ggtaatgcat ttgaaaccgt gcaagccgat
    25261 tcagaaaaat tggtgctgac cttccgtgtg acggcttttg acgcagtggc ggcgcaaggt
    25321 ggtaatgctg aggtaattaa tgttaaaaca acccctgccc aaaacctgag tcgttttgtt
    25381 aaccgtcaac tttcccattc cgatcgtccc gaactgactc aggcaaaagt gattgtttcc
    25441 ggtggtcgtg cgttgggtag tgtggaaaaa ttcagtgaag tgctgacacc gttggcggat
    25501 gttttaggtg cggcaatcgg tgcatcccgt gcggcggttg atgccgagta tgcaccaaac
    25561 gatgctcaag tcggacaaac cggtaaggtg gttgcgccgc agctctattt tgcaatcggt
    25621 atttcaggtg cgattcaaca cgttgccggt atgcaggaca gtaaggtgat tgttgcaatc
    25681 aacaaagatg ccgatgcgcc gattttcaat gtagccgatt acggattggt tggcgattta
    25741 tttgaaattg ttccacaatt aatagaatta ttgaaaaatt gatgtttcac gtgaaacttt
    25801 caggcgactt tgttattaat gaggtcgtct gaaaacattt cagcatgatt acgacttata
    25861 aaaccattac ttccccgacg caggctgagt ttaaagataa aggcagccgt tttattgcat
    25921 ttgcctatcc gattcggaca ttggctgatg tgaaaaaata cctcaatctg ttaaaggaag
    25981 agcattataa agcgcggcat tggtgctatg cctatcgttt aggtgtgaac gggatgcagt
    26041 ttcgcggcaa cgatgatgga gagccaccag gaagtgctgg gcggccaatt ttgggacaga
    26101 ttgattcggt gggtatcact gatgttttgg tcgtggtcgt ccgctatttc ggtggtgctt
    26161 tattgggcgt tcccggcttg attcacgcat acaaagaggc aacggctcag gcgttggcga
    26221 tcgcggaagt ggttgaaaag aatattgaaa aaaccgtttg gctgaaatgt gagtatccgt
    26281 ttttgaatga agcaatatgc attgccaaac aatatcaggc ggatatattg gagcaggatt
    26341 tgcagttgga ttgccgattg acggtaagtt tgtcgttggt aaattatgag gcctgtgttg
    26401 cggcttggaa gaatacacgg caggttgaag taaatacgga aaagcctttt gaatgaaagg
    26461 ttgtatgaaa tgctttgtaa tggtatttca ggcaacctct tcgctgatta attttcttgt
    26521 gtatatctgt ctaaggtatc gacgctggag cataaatata gccaagccaa ttgatggaat
    26581 tcgcccggcg cgttccataa tgcatcttca ctcataatgc tatattcgcc ttctgtttgc
    26641 aaattcagtt ctaccccgtc ttcaatagtc tgatggcgca cctgatactc gtgggtatag
    26701 aaattgtcca tatcgcgaat taaagcgacg gcatatttcc aacggcggat gttttcttcc
    26761 agttgcagta gtaaatgaca ccattcgcgg tatttgcttt ggatttcatc aatacgtttt
    26821 tgcgtgtttg cttgttcttt tttaatttgt gaataagttg aataggttgc agagtggaat
    26881 gaagtggtct tttgtttgat tctactaaaa tttcttcaga tgggacaaaa gttcctttgt
    26941 atcgagtttt cgttaaaagt acatatttgt caaggagatg gcatgaaact gctggttatc
    27001 ggtaatggcg gtcgcgaaca cgcgctggct tggaagttgg cgcagtcgcc caaagtggaa
    27061 acagtatttg ttgcgcccgg taatgccggt acggcgattg aatccaagct ccaaaacatt
    27121 gccttgaccg cgtatcagga tttgattgaa ttctgccgta aagaaaatat tgtttttacc
    27181 gtcgtcggcc ctgaagcgcc tttggcggca ggtattgtgg atgattttcg tgccgcaggg
    27241 ctgaaaatat ttggtccgac acaatatgcg gcgcagttgg aaagttctaa agatttcgcc
    27301 aaagcattta tggtgaaata caatattccg accgcgcaat accaaacctt tgaaaatgcc
    27361 gatgccgcac atgattacgt caatcagaaa ggtgcgccta tcgttatcaa agccgatggt
    27421 ttggtggcag gtaaaggcgt gattgtggca atgactttgg atgaagcgca tgctgcgatt
    27481 gacgatatgt tgttgggcaa taaaatgggc aatgccggcg aacgcgttgt gattgaagat
    27541 tttctgcaag gcgaagaagc gagctttatc gttatggttg atggcaatca tgtattacct
    27601 atggcgacca gtcgagacca caaacgtctt ttagacggag ataaaggccc gaatacgggc
    27661 ggtatgggtg cgtacagtcc cgcgcctgtg gtaacgcctg ctgtgtacga gcgtgcgatg
    27721 aacgagatta ttttgccgac cgtagcgggt atgaaagcgg aggggcacga gttcaccggc
    27781 tttttgtacg caggtttgat gatcgatcaa agcggtgcac cctatacgat tgagtttaac
    27841 tgccgtttcg gcgatcccga aacccagccg attatgagcc gtctaaacag cgatttggcg
    27901 gatttggttg aagcggcaat agacggcaga ctggacagcg tcaaagcgga atggaatccg
    27961 caaactgccg tgggtgtggt actggcggcg caaaattacc ccgaaacacc caagaaaggc
    28021 gatgttattt ccggtttgga tgacgtcaac cgaatcggca aagtattcca tgccggcaca
    28081 acggtaaacg agaaaggcga cgtgttgacc aacggcgggc gcatattgtg tgttgtagga
    28141 ttgggcgacg atgtggcgca agcgaaagcc aaggcgtatg gcgcgttgga aaaaatcagc
    28201 ttcgacggga tgcaataccg taaagacatt gccgataaag cgattaaccg ttaattagaa
    28261 agaggatgat gccgtctgaa gccaagtatg actgatgcgc ttcagacggc attttatatt
    28321 atgttatttt cgagaattat cgcagcatca gcgcaacgta agttaagcgt ttatttgaaa
    28381 aaaggcggtt tggttgctta tccgaccgaa tcttgttatg gcttggggtg tcttccgaca
    28441 ttagccaaag cactcggtaa gttggcgcat ttgaaaaaaa ggccgcaaca caagggtatg
    28501 attgtcatcg ggaatcaact tgaacaattg cagcctttac ttcaaatgcc gtttgaaaac
    28561 attcaaacca tgcttagaaa cgaatggcct gctcctaaga ctttcctatt gttggctaaa
    28621 tctggcgtat tacctgcgtt gcgcgggaag aggcgcagca agcttgcggt cagagtgccc
    28681 gatcatacag gtgcaaggcg tttgtgtcag gcattgggta tgcctttggt ttccacttca
    28741 tgtaatcggg cgggtaaacg cgcgtgtcgg acggagaggg aagtcagaag gcagttcgga
    28801 cgcgatgtgt ggattgtcgg tgggagaatc ggcaggcaga aatcgccgag tcagattatc
    28861 gatggggaaa caggtaaacg tttgcgttag atatggatgc ttgagtgaaa acttttttgc
    28921 atacacaaag aaaaaatttt ttggatggta tttctctaca tgattgttat cgcacggcga
    28981 taaaggtaga aggacgcaac gtctgctttg attttgagga cggttttgtc gttctggaca
    29041 ataatccgaa caatcaagcg ggcaagcatc tgaaaacaga tttttcccgt gttgttttaa
    29101 cccatgaaaa tagaaacgcc gaagacgact atttggtcga tatttttgaa gacatcgtct
    29161 ttttagggaa acgtttgttt acagtcagaa aatttcttga tttttctgaa ttgcttgaaa
    29221 tgatcaatgc acaaggctat tttcttgaat ttctgtatct gtatgaatgt atcggtgtca
    29281 aaacaagcga ttatttttta gaggctgtgt tggttacggg acggagccgg gaatgtaaaa
    29341 aatgctttat caaaattatg agggcaagca gttttgtgta tcaatggaac aatttacgtt
    29401 tgaatagtga gattgtatag cgggtttata tttcggatgg aagccggttt aaaaaaataa
    29461 tgtggataat ttcagggaat ggcaaaatgt cgtctgaaat gctgatattt cagtattcca
    29521 gacggcattt ttataataag gcttatggat aaatttgtgt ataagttaaa atcagagttt
    29581 tttcccgcat acggtttcat attgtttttc agttaatgtc ccggacagga tgctcatcgg
    29641 gcggagggaa gaggctgtgt agttttgtgt ggaaatttcc gtgttttttg tatcaaataa
    29701 ctgtagcgaa cttaagcggt atgttttgtt gttgcagtgg atttcccact cggcaatggc
    29761 agtcttgtat gcgggggtgt tggcaaaacg ttcttgtttc agattggtaa caactttttt
    29821 atcttggaaa atcatcagat ttccgttttt tctcacactg tctttattaa tatatgtatg
    29881 aatattgccg tttgagattg tgccgatatt ttcccatcgg gcagacttgt ccggtgtgct
    29941 gcaggcggtt atcaaaatag ggagtaaaac gaaagatagg aagattgggc gcattagggt
    30001 ttccttaatc attgaaatcc gctcattcag aaagaacggg ttgggccgga ggggtaatgc
    30061 cgtctgtgaa atgaggcaaa tatgggaaat tgatgcagcc ttcgtgtttt acgtatttat
    30121 atattccgta tggaggaata tgctgctttt gaagagtgta ttatggatga actgccgata
    30181 acagtaaatc ggtttgattt gtttccgtca gcttatgccg tctgaagccc tttcagacgg
    30241 catttatttt gaagttctgg ttgtttttgc cagtgatatt ttgatggttt gaagctgtct
    30301 tttatattcc cggcaatgcg ggcagaacag caggtgcata tatatggaaa tcttctcgcc
    30361 cggggtggtt tcccggtcct gatgtttgga aagaagcagg gcaatatcgc ggcatttttt
    30421 cataacgttt acttcgggtt ttcttggttg aaccatttga tttgcaggca ttggcgcaat
    30481 gattctcggg cgcggtgcat aatggtgtgg tagttggacg tgctgatacc gcacatttgt
    30541 tgtatttcgt tggatgaaaa accgagtatt tccttcaggg tgaatacccg tgcggtgttt
    30601 tcaggcaggt tgtataggca gctttgcaga attttttgga attcgttgtt atttaatgat
    30661 ttttccggag tgttccaatg ttgcggctgc ccttccggcg tccaatgccc gttttgggaa
    30721 aaatggcttt caaatgcttc atccagcagc tcgtcatcca gtgcggtaaa gactttcctc
    30781 tgcctcccga tttgacgtaa tgcgtcaata attttgtttt tcaatatggc aaaaagccag
    30841 ctgttgacaa gtgccctgcc ttgaaaactg tcgcctgcgc tgtatgcgga cagcaatgtt
    30901 tcctgcacta aatcttcagc caaatcaggg tggtcgggca actggagtcg cgcaaaatgc
    30961 agaagcagtt tgcgcgactc cattaattcg gcatcggtca ggtcgggtag cggcatagta
    31021 tcgggaacaa ggtaatttta tgcggataga tggttttggc tcggggaatt gccttccata
    31081 ttttcggata tggcggacaa ggaggggatg ataatccttt gttcaatcca gcctgaccat
    31141 ttcccaagca aaatattttt ccaattatcg gctttaggca taaattcgac aagggtttgg
    31201 gcgagggtgt caaacgaaag cgcggaggaa cccattatct ccagcagcat catatcgaag
    31261 ccgtccaatg tttggtacat cacatcatct tcggcgtttc gccatattaa caaggctgtt
    31321 tccgcttcct gcaaatcatg ggtaacatcg tatcgatatt gccggataaa ggccgcaggg
    31381 gagggcgtgt atttgctgtc atttgaataa tgaatgtcgg gaatatccgg aatttgtgca
    31441 acttctgcca gcaattgggt atattcaaaa tccatcagtg ccaaaatgcc gtctgaaagc
    31501 ggcaggcttt ggcaatattg taggaactcg ccggggattt cttgaaaata gggcgtttgg
    31561 gcacgcgcgt cgcggacgaa accttctttc agacggctcc attctttgct gtcaaagtat
    31621 tgccgcgttt cggtatagca gcggtcgata aagctgtgga tattgttgcg tatcaggcgg
    31681 atatagacgt tcaatcggtc ttgcggcaga ccgtctgcgg cttcgccccc gcgtatggct
    31741 tgggagaaac ggtgctggta ttgggcggag gtttcaggct gcacggcggt attcctttcc
    31801 ggcacgcgtt tgataatcgg caattttggc aacttcggct tcgagttcgg caaaaggtgg
    31861 gaaattgaaa tcgcgttcca acagggtggg agggatggtg ggaagcttgg tgtaggcgag
    31921 ttcgagcaag tcccaaacgg tcggtaaaac cgccgcgccg tgggtatcaa tcaaaagctc
    31981 gggggtttcg gcatcgtgtc cggcgatgtg gatgtagcac acgcgccctg cgtccacatt
    32041 ctctaaaaag gcctcaggcg acagcaggcc gtgattgacg gcgttgacgt aaatattgtt
    32101 cacatccaaa tgaataccgc agtcggcttc acgggcaacc gcgttgagga actcgacctc
    32161 gttcatctcg gcaagcggag aatgcaggta gtaggacgtg ttttccacgg cgatgcggca
    32221 gcccaaacgg tcttgcactt cgcggatacg ccgcgccgta tgatgcacca tttcttcagt
    32281 gaaaggcagc ggcaacaaat cgtaaagatg accgccgtcg tggcagtagc tcaaatgatc
    32341 ggagaaaaac gtgcagtcat aacggcacat catttctttg atgccgtcta tcaaatcggt
    32401 atccagcggg gcttgtccgc ccagcgacat agataatccg tgcaacgcca gcggcagccg
    32461 ttccgccaca cggtcaaact gtttgcgcgc cctgccgccc attttcagcc agttttccgg
    32521 tgcggcttcg ataaagcata tcgggctgtt ttcggaaagc gagagaaagt cttccgccaa
    32581 gtcgcggcgg tagcccaagc ctgcgtgttg aatcatattt ttacccggtt cggatggcac
    32641 aaaagaataa tgtgcttgtt taaaaaaatc tttgtttgcc gttaaaaaag ccgcatttta
    32701 ctgaaaaacg cggcttggtt tgaaggggag ggattattta gaaccgcatt tgccttcgcc
    32761 gcatttgcct tcggcagatt tggctttggc tttagatgct ttggtgtgtt tgtgggcttt
    32821 ttttacagtt gcaccgcatt tgccctcgcc gcatttgcct tcgcctgctt tagaagcagc
    32881 cgcgccgcac gaaccttcgg cagatttgga tgcgccgcaa gagccttggg cggatttttg
    32941 aacgcctgtt gcgttgcttg ccggtttgtg ggcggcaacg gcgccggcgg ccaaagacag
    33001 ggataaagca ccggcgagtg cggcagcaat atttttgttc atttctattt ccttttcaga
    33061 atatcaagtt aacgagataa ggttggaatg tttaaagtcg gattccacga ctgaatcaat
    33121 cttgtttcag acggcatttg gggcaaaaca gttttttcag cagcgtatcc agcgaccatc
    33181 cgcccgcacc ctggaaaagc agcgggatta ataccacgat ataaattaaa gccattttat
    33241 aaccgttgtc gcagacattg taacccgaac cggcgtgaac cgcagcccaa gcgacggcgg
    33301 taacgaccat cagccccaat gccgacaggc gcgttgccaa acccaaaagc aacagtacgg
    33361 gcagcaaaag ctccgcatac atggcgaaat tccagtttaa cgcgtccggc agcaagttga
    33421 acgggaatgg aaactgatcg ttgatttcgg aaaaccaatt ctccccgttc catttttgca
    33481 aacccgattc caaaaattca tatgcggcaa acagacgcaa aaccagcata gcggatgccg
    33541 tctgccaagt tgttccgatt ttcataagtg ttcctgtttt tgttcctgtt gataccccgt
    33601 tagacggatt ttgtcggaaa aactgacacc cgtcagctaa taatttaaca agttaatgaa
    33661 tataaaaaga aagtattttg acggaaagaa agggaaaaag gcgggatatt tggataacgg
    33721 cggtgttgtg agataattcc cgccttggac ggtatgcctt caaatacaat ttccgtaaag
    33781 gaaaaaagaa aatattaatt ttgatgatga tttaacaaag cccctttcgc ttggctgtca
    33841 aaggagattg ttaagaaaag caatctccct ttgacggggt gcgatatgta aaggaatgga
    33901 aaatatggta acgttttcaa aaatcagatc atttttggca atcgccgccg ccgcgttgct
    33961 tgccgcctgc ggtacggcgg gaaacaatgc cgcccgcaag ccggtgcaaa ccgccaaacc
    34021 cgccgcagtg gtcgctttgg cactcggtgg cggcgcatct aaaggatttg cccatatagg
    34081 aattgttaag gttttgaaag aaaacggtat tcctgtgaag gtggttaccg gcacatcggc
    34141 aggttcgata gtcggcagcc ttttggcatc gggtatgtcg cccgaccgcc tcgaattgga
    34201 agccgagatt ttaggtaaaa ccgatttagt cgatttaacc ttgtccacca gtggttttat
    34261 caaaggcgaa aagctgcaaa attacatcaa ccgaaaagtc ggcggcaggc agattcagca
    34321 gtttcccatc aaatttgccg ccgttgccac tgattttgaa accggcaagg ccgtcgcttt
    34381 caatcagggg aatgccgggc aggcggttcg tgcttccgcc gccattccca atgtgttcca
    34441 gccagtcatc atcggcaggc acaaatatgt tgacggcggt ctgtcgcagc ccgtgcccgt
    34501 cagtgccgct cggcggcagg gggcgaattt cgtgattgcc gtcgatattt ccgcacgtcc
    34561 gagcaaaaat gtcggtcaag gtttcttctc ttatctcgat cagacgctga acgtgatgag
    34621 cgtttccgtg ttgcaaaacg agttggggca ggcggatgtg gttatcaaac cgcaggtttt
    34681 ggatttgggt gcagtcggcg gattcgatca gaaaaagcgc gccatccggt tgggcgagga
    34741 ggcagcacgt gccgcattgc ctgaaatcaa acgcaaactg gcggcatacc gttattgacg
    34801 ctatgccgtc cgaacgtgtt tccagaaggc tttatccata gaaaaaaata cccgaatccc
    34861 gtacaatacg cccctaaaat tttgaaagaa cgaaagaatc atggaagccg aagtaatcaa
    34921 ccagctcaac aacaccctga atgatttgga gaagcgtagc gaagacatcc gcgtttatat
    34981 ggattatcag ggcaagaaag accgattgga agaagtcatc gggctttccg aagaccccga
    35041 gctttggaac gacccgaaac gtgcccaaga aatcggcaaa gagagcaaaa tccttgaagg
    35101 catcgtgctg acgctcgaca acatcgcttc gggtatcgaa gacaaccgta tgctgattga
    35161 aatggccgtc gaagaaaacg acgaagaagg ttttgccgcc gtgaaggaag acgtggcggg
    35221 gctggaaaaa cagatggcgg atttggagtt caaacggatg ttcaaccagc ccgccgaccc
    35281 gaacaactgc tttatcgaca tcaccgccgg cgcgggcggt accgaagcgg aagactgggc
    35341 ggggatgttg ttccgtatgt acagccgcta tgccgagcgc aaaggcttca aaatcgaaat
    35401 ccttgaggaa gacgacggcg aaatcgcagg catcaaccgc gccaccatcc gagtggaagg
    35461 cgaatacgct tatggtttgc tgcgtaccga aaccggcgtt caccgcttgg ttcgctactc
    35521 gccgtttgat tcgaacaaca aacgccatac ctcgtttgcc tccgtgttcg tttaccctga
    35581 aatcgacgat tccatcgaaa tcgaaatcaa ccccgccgat ttgcgcatcg acacctaccg
    35641 tgcatcgggc gcgggcggtc agcacatcaa caaaaccgac tccgccgtgc gtattaccca
    35701 cgagccgacg gggattgtgg tgcaatgtca aaacgaccgt tctcaacacg ccaacaaagc
    35761 cgccgcgatg gaaatgttga aatccaaact gtatgaattg gaaatgcgca aacgcaacga
    35821 agagaaacag gcattggaag aaggtaagtc ggatgtgggt tggggcagcc aaatccgttc
    35881 gtatgttttg gattcctcac gcatcaaaga cttgcgtaca ggctacgaag tcggcaacac
    35941 caaagccgta ttggacggcg acttggacgg ctttatcgaa gccagcctga aacagggtgt
    36001 gtagtttagg ctgtatgccg tctgaaacct gtaccgtttc agacggcatt tttgcgtttc
    36061 gtgttataat ccgcgccgca cacgggacgg gcagacagtc gccgcgtatc gcgtaaggca
    36121 tacggggagg aaagtccggg ctccgcaggg tagaatgccg gttaacggcc gggcgcggta
    36181 acgcgacgga aagtggaaca gaaagcaaaa ccgccgatgg ctgctttggc agcacaggca
    36241 agggtgaaaa ggtgcggtaa gggcgcaccg cgcatttggt aacaatatgc ggcaggccaa
    36301 accccattcg gagcaagacc aaacagaacg caatgacgct gcccgccgag cgttcgggta
    36361 ggttgcttga gcataccggc aacggtatgc ctagaggaat gactgtccga gacagaaccc
    36421 ggcttaccgc ctgtccggtg tgcatccgac acgcaaatgc cgtctgaaac ctgaaatacg
    36481 gttcggacgg catttttatt taggctgtgt ttttatattc cgttgatgca gccctgatga
    36541 gtcggacaaa atgcctttta ctcaatagga ccggtttccg cccaagcgga aataaacggc
    36601 tatttccctt gcaaacagac ggaatcaggc gggtatttaa atgacaatcg gcaggctgcc
    36661 ttccggttgg gcgggagcct gtcctgtcgg gtggaaacgg tttggtttca ttatgcggaa
    36721 aaatgccgtc tgaagggttc agacggcatc ggcgcatcgg ggcaggactt agtctgtgcc
    36781 ttcgagcatt ttgttcaatt tggggacgag caggagcagc aggaagcctg tggctgcgcc
    36841 gatgtagaac agcagccggt agaagccgat ttcgtcgccc gcctgatagc ctttttcaaa
    36901 caatacgccg cccaaagtga agcctaatga aaaggcaagg aaattaaggg cgaccatttg
    36961 ggttttgaat aaaggcggtg cgattttggt ggagatggac agcgcaatcg gggaaatcat
    37021 cagttcgcct atcgtgatgg cgaggacgat cagtgcgaaa accgcaatag gcatcggcgt
    37081 tccggaggaa ataaagggaa cgaatcccaa aaacgacgcg ccggtaacaa ataccgccat
    37141 agcgaatttc agcggggttt tgggttgttt gcgccccatt tttgtccaca ttgccgccat
    37201 gagtccggaa aacaggatga cccacaggct ttgcatagaa tctttccagg cgacgggtac
    37261 ggtaaacgaa ccgatggtgc ggttgacggt ttcgtcgaaa tagacggttg ccacggtgta
    37321 aatctgaaac cagacggccc aaaacataca gatggtcagg aaaagcggga tgtaggcgat
    37381 gatgtgccgt ttgttgtcgg aactgacgcg ggggttggtc agcaggcggg cgaaataggc
    37441 gatgacggca aggatgacgg tagataagag gatgccggag aaattgtcga ggttgacaag
    37501 cccggttttg atggcggttg caagtgcggc gatgagggtg atgccgacgg cggccgcagt
    37561 tttgccctgt ccttttgaaa gcggatgggg gacggtgggg tggggcaggt ttttacgtcc
    37621 caaggaataa cgccacaagc cgaatgccat accgaccgcc gccgcgccga aaccataatg
    37681 gaaaccgatg ttttcttgaa gcagtccggt caataagggg cctaagaagc cgccgatatt
    37741 gatggcgatg tagaaaatgg aaaatcccgc atcgcgcagc gggcgcattt cgtcctgttc
    37801 gtataatgcg cccaccatag aactggccgt agatttcacg ccgccgctgc ccaatgcgat
    37861 gaatatcagc ccgattagaa ggccgtacag gcccggtgcg gcggcaagga cgatgtgccc
    37921 gagcattacg acgatgcccg agaggaagag ggttttttcc gcaccccata cgcggtcggc
    37981 aaaccacgcg cccaaaatgg tggacaggta cacgctgccg ctgtatgcgc cgacaatgcc
    38041 gcctgcgagg gttttgtcta tgcccaagcc gcctttgtcg gcggtgtagt agaggtaaat
    38101 cagcaggatg ccctgcattc cgtaaaatga aaaacgttcc cacaattcga tatggaagag
    38161 ggtggaaagc tggaaggggt ggccgaaaaa tgttttttct ccggtgcggg cgggatgccg
    38221 ggacataaag gttctcctaa aagattttct ctcaataccg tctgaaacgg atgcggacgg
    38281 cggcggtgtt tttttgtatg aaaatatcgg tttttaaccg atattttcat tctttgtcaa
    38341 acgacgcgct gccgtttttc gcgggcggat gtttttatat ttgtttcaat tcaatggatt
    38401 gtattttaga ggacgtgttc cgatacggcg cgggtaaatc cttttctgtc aatggcttat
    38461 ccgatagggc ggtttttact tagatgggaa atcacttcct tatatatccg ccacgccgaa
    38521 gggcgcatta tggtggatta actttaaacc ggtacggcgt tgccccgccc cggctcaaag
    38581 ggaacggttc cctaaggcgc ccaagcaccg ggcgaaccgg ttccgtacca tttgtactgc
    38641 ctgcggcccg ccgc
//
LOCUS       DAWTCI010000009        38071 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWTCI010000009 DAWTCI010000000
VERSION     DAWTCI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38071
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.009"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(80..571)
                     /locus_tag="WM481_000505"
     CDS             complement(80..571)
                     /locus_tag="WM481_000505"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HFA9079892.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQRLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(645..1403)
                     /locus_tag="WM481_000506"
     CDS             complement(645..1403)
                     /locus_tag="WM481_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9079893.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQRPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAGMNRGDVTRNPVKLGERYYLFKLGAVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(1473..2339)
                     /locus_tag="WM481_000507"
     CDS             complement(1473..2339)
                     /locus_tag="WM481_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9079894.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAVYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKRTGAPDGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(2388..2666)
                     /locus_tag="WM481_000508"
     CDS             complement(2388..2666)
                     /locus_tag="WM481_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HFA9079895.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGLSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(2666..2956)
                     /locus_tag="WM481_000509"
     CDS             complement(2666..2956)
                     /locus_tag="WM481_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFA9079896.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHFKRLETLKSEGRLLTAGPN
                     LLPDNPERVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(2959..3489)
                     /locus_tag="WM481_000510"
     CDS             complement(2959..3489)
                     /locus_tag="WM481_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFA9079897.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAAAAVALVAGVVQAAFLYWK
                     HKRLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPTVLFWCGALFLLGSHLAGKNGLK
                     ASIGREIQLPDAVWGKLTYMWVGFLIFMGIANWFVFTRFEAQWVNYKMFGSTALMLFF
                     FIIQGIYLSTYLKKED"
     gene            3797..4822
                     /locus_tag="WM481_000511"
     CDS             3797..4822
                     /locus_tag="WM481_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9079898.1"
                     /translation="MKLIAASIAVAASFQAHARLGGLNIRSNLDEPFSGSITVTGEEA
                     KALLGGGSVTVSEKGLTAKVHKLDDKAVIAVSSAQAVRDPVLVFRIGAGAQVREYTAI
                     LDPVGCSPKTKSALSDGKTHRKTAPKAESQENQNAKALRKTDKKDSANSAVKPAHNGK
                     THTVRKGETLKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRI
                     KAAAPKKIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPIEKPVEKPVEKPEAKVAA
                     PEAKAEKPAVRPEPKPAVSETPASATERQPGPVPAANTAASETAAESAPPRSRRFCHR
                     HADRRNR"
     gene            4959..6113
                     /locus_tag="WM481_000512"
     CDS             4959..6113
                     /locus_tag="WM481_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HFA9079899.1"
                     /translation="MLLAGGGTALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAAD
                     DGIKITFAEVETPATPEPAPKNDVNDTLALGGESEEELSAKQTFDVETDTPSNRIDLD
                     FDSLAAAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTP
                     EPESVAQTAENKPETVDTDFYNNLFSNNHIGTEETASAKPAAPSGLAGFLKASSPETI
                     LEKTVAEVQTPEELHDFLKVYETGAVAETAPETPDFNAAADDLSALLQPAEAPAVEEN
                     AAEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSD
                     APSEAKYDLAEMHLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(6286..6702)
                     /gene="gloA"
                     /locus_tag="WM481_000513"
     CDS             complement(6286..6702)
                     /gene="gloA"
                     /locus_tag="WM481_000513"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFA9079900.1"
                     /translation="MRLLHTMLRVGNLEKSLDSYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGDAYGHIAVEVDDAYEACERVKRQGGNVVREAGL
                     MKHGTTVIAFVEDPDGCKIEFVQKKSGDDSVAYANT"
     gene            complement(6727..7896)
                     /gene="lapB"
                     /locus_tag="WM481_000514"
     CDS             complement(6727..7896)
                     /gene="lapB"
                     /locus_tag="WM481_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFA9079901.1"
                     /translation="MDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETAQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(7907..8221)
                     /locus_tag="WM481_000515"
     CDS             complement(7907..8221)
                     /locus_tag="WM481_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HFA9079902.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAK
                     QP"
     gene            8531..9529
                     /gene="ilvE"
                     /locus_tag="WM481_000516"
     CDS             8531..9529
                     /gene="ilvE"
                     /locus_tag="WM481_000516"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFA9079903.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLTLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWLLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKNQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(9774..10559)
                     /gene="fabI"
                     /locus_tag="WM481_000517"
     CDS             complement(9774..10559)
                     /gene="fabI"
                     /locus_tag="WM481_000517"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFA9079904.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(10714..11535)
                     /gene="dapD"
                     /locus_tag="WM481_000518"
     CDS             complement(10714..11535)
                     /gene="dapD"
                     /locus_tag="WM481_000518"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFA9079905.1"
                     /translation="MSLQNIIETAFENRADITPTTVAPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNAVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGAIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSLPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(11745..13388)
                     /gene="pgi"
                     /locus_tag="WM481_000519"
     CDS             complement(11745..13388)
                     /gene="pgi"
                     /locus_tag="WM481_000519"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9079906.1"
                     /translation="MDAFTRAWYALERHYQDTCHILLRDRFAAEPDRFERMHERLDGM
                     LFDYSKNRFGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGLYQGITGKRIADFVHIGIGGSDLGPAM
                     CVQALEPFRRQISVHFVSNADPACLDEVLCRLNPETTMFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGAEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPCNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            13898..15130
                     /gene="pilG"
                     /locus_tag="WM481_000520"
     CDS             13898..15130
                     /gene="pilG"
                     /locus_tag="WM481_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFA9079907.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            15204..16064
                     /locus_tag="WM481_000521"
     CDS             15204..16064
                     /locus_tag="WM481_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFA9079908.1"
                     /translation="MSDLSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEHLNLPLTDDESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYSSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGRHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVR"
     gene            16061..16693
                     /locus_tag="WM481_000522"
     CDS             16061..16693
                     /locus_tag="WM481_000522"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFA9079909.1"
                     /translation="MTAWVGLTGGIGSGKSAAAQYFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMLLHAFYSGIFASKPTQGKHNG"
     gene            16686..16895
                     /locus_tag="WM481_000523"
     CDS             16686..16895
                     /locus_tag="WM481_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFA9079910.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDGAYR"
     gene            complement(16981..18657)
                     /gene="pilB"
                     /locus_tag="WM481_000524"
     CDS             complement(16981..18657)
                     /gene="pilB"
                     /locus_tag="WM481_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFA9079911.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVGILDVAYKEGMVDLRRAGILKIMQGITSLEEVTANTND"
     gene            complement(18903..18974)
                     /locus_tag="WM481_000525"
     tRNA            complement(18903..18974)
                     /locus_tag="WM481_000525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18939..18941),aa:Arg,seq:cct)
     gene            complement(18995..19441)
                     /locus_tag="WM481_000526"
     CDS             complement(18995..19441)
                     /locus_tag="WM481_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFA9079912.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITVSATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGCG
                     VPLMGRQPVLVTGLLIGTVIGVAFMGGIPVGPLIAADILSFVAGKV"
     gene            complement(19445..20419)
                     /locus_tag="WM481_000527"
     CDS             complement(19445..20419)
                     /locus_tag="WM481_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9079913.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIRVIQYKTAKLFEAAAQVGAILGKASPGHEQALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRGYFEKIRDYV
                     VRSDALAYSIGEARKAVDCAVAALDALSDSEVKDAMIQLAKESLVRVS"
     gene            complement(20435..20623)
                     /locus_tag="WM481_000528"
     CDS             complement(20435..20623)
                     /locus_tag="WM481_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079914.1"
                     /translation="MVNIRTPLCEIGHCIVICLFCQSLRGSGNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            20643..20951
                     /gene="rplU"
                     /locus_tag="WM481_000529"
     CDS             20643..20951
                     /gene="rplU"
                     /locus_tag="WM481_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFA9079915.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAQLDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            20976..21248
                     /gene="rpmA"
                     /locus_tag="WM481_000530"
     CDS             20976..21248
                     /gene="rpmA"
                     /locus_tag="WM481_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFA9079916.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKIDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            21483..22667
                     /gene="ubiM"
                     /locus_tag="WM481_000531"
     CDS             21483..22667
                     /gene="ubiM"
                     /locus_tag="WM481_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFA9079917.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEIVSQLDNVSILTDTAVKEVKTSDNEAQVFLENGDILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEKRLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(22856..23011)
                     /gene="rpmG"
                     /locus_tag="WM481_000532"
     CDS             complement(22856..23011)
                     /gene="rpmG"
                     /locus_tag="WM481_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFA9079918.1"
                     /translation="MRDKIKLESGAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(23043..23276)
                     /gene="rpmB"
                     /locus_tag="WM481_000533"
     CDS             complement(23043..23276)
                     /gene="rpmB"
                     /locus_tag="WM481_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFA9079919.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(23526..25049)
                     /gene="farB"
                     /locus_tag="WM481_000534"
     CDS             complement(23526..25049)
                     /gene="farB"
                     /locus_tag="WM481_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFA9079920.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFSVANAVSVPLTGFLAKRIGEVKLFTAAAAGFVIASWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRMLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKHRETATVRTPTDYVGLTLMMVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSAWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDGQTLGIINNTITQQGFIIGSNEIFLAGSILFIVLIPIVWLAKPPFHSGGGGH"
     gene            complement(25073..26227)
                     /gene="farA"
                     /locus_tag="WM481_000535"
     CDS             complement(25073..26227)
                     /gene="farA"
                     /locus_tag="WM481_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFA9079921.1"
                     /translation="METHTDETKLQNTQVKRKRRLTALTLLFALSAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RTAVSQAQAAVKAALAEESSARAALGGDVSLREQPEVQTAIGRLKDAWLNLRRTQVRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      26468..26512
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            26516..26989
                     /gene="queF"
                     /locus_tag="WM481_000536"
     CDS             26516..26989
                     /gene="queF"
                     /locus_tag="WM481_000536"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFA9079922.1"
                     /translation="MSRNNEELQGISLLGNQKTQYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAVHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            27064..27750
                     /locus_tag="WM481_000537"
     CDS             27064..27750
                     /locus_tag="WM481_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFA9079923.1"
                     /translation="MYALTAAQQQKALFRLVLFHILIIAASNYLVQFPFRIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     QFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWIAPAASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCTLFFLPAYGVILNLLTKKLTALQTKQAQ
                     DRPVPSLQNP"
     gene            complement(28100..29392)
                     /locus_tag="WM481_000538"
     CDS             complement(28100..29392)
                     /locus_tag="WM481_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFA9079924.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTEGTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGSAREVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQ
                     VAAGDILAAEVVKGDTTHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHS
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            29469..30437
                     /locus_tag="WM481_000539"
     CDS             29469..30437
                     /locus_tag="WM481_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079925.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSPHRQSCTASA
                     FYARYLWCGRLAQRPAQSLNMPSETVALATPVWQKMGLHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLNAHPVNHNRKKRGLPELNGLWLWDSLHGSAQGGTLFADTVWSRFH
                     PNRRALPDSFRAYAETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRTGKIKRLDIATDGQHGGTLTFKPTDRRKFWRCTKTFDGIW"
     gene            complement(30638..32107)
                     /locus_tag="WM481_000540"
     CDS             complement(30638..32107)
                     /locus_tag="WM481_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9079926.1"
                     /translation="MVIFYFYFCGKTFMPARNRWMLLPLLASAAYAEETPREPDLRSR
                     PEFRLHEAEVKPIDREKVPGQVREKGKVLQVDGETLLKNPELLSRAMYSAVVSNNIAG
                     IRVILPIYLQQARQDKMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLA
                     AALFEDRQNEAAADQFDRLKTEDLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHN
                     INQAPKQQQYGNWTFPKQVDGTAVNYRFGAEKKWSLKNGWYTTAGGDVSGRVYPGNKK
                     FNDMTAGVSGGIGFADRRKDVGLAVFHERRTYGNDAYSYANGARLYFNRWQTPRWQTL
                     SSAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWTGGLDFYRERNPADRGDNFNR
                     YGLRFAWGQEWGGSGLSSLFRLGVAKRHYEKPGFFSSFKGERRRDKESDTSLSLWHRA
                     LHFKGITPRLTLSHRETWSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(32349..33387)
                     /locus_tag="WM481_000541"
                     /pseudo
     CDS             complement(32349..33387)
                     /locus_tag="WM481_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(33727..33972)
                     /locus_tag="WM481_000542"
     CDS             complement(33727..33972)
                     /locus_tag="WM481_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079927.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGLDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            34084..34512
                     /locus_tag="WM481_000543"
     CDS             34084..34512
                     /locus_tag="WM481_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFA9079928.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGRWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            34524..35000
                     /locus_tag="WM481_000544"
     CDS             34524..35000
                     /locus_tag="WM481_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFA9079929.1"
                     /translation="MPIEIERRFLIENDKWRQYADEPLLLKQGYLSVEKERTIRIRIA
                     GKRAWLTLKGYISEISRSECEYEIPLADAEKMMETMCPFKMEKRRYPVRWGGSLFEVD
                     VFLGENSPLVVAEIELPAENADFDRPDWLGREITSDGMFTNAYLSKHPFSSWKNAV"
     gene            complement(35030..35518)
                     /locus_tag="WM481_000545"
     CDS             complement(35030..35518)
                     /locus_tag="WM481_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFA9079930.1"
                     /translation="MLKITIIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETVASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAERTERRVSSKGVAYTFVHYL
                     KG"
     gene            complement(35586..36641)
                     /gene="aroG"
                     /locus_tag="WM481_000546"
     CDS             complement(35586..36641)
                     /gene="aroG"
                     /locus_tag="WM481_000546"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFA9079931.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDRRLLVIIGPCSIHDPKAALEYAERLLKLRKRYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAVEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIADQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANTKRMARAG"
     gene            complement(36803..37030)
                     /locus_tag="WM481_000547"
     CDS             complement(36803..37030)
                     /locus_tag="WM481_000547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079932.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFARMNGGYCFYQSVSFSLK"
     gene            36966..37216
                     /locus_tag="WM481_000548"
                     /pseudo
     CDS             36966..37216
                     /locus_tag="WM481_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37258..37554
                     /locus_tag="WM481_000549"
     CDS             37258..37554
                     /locus_tag="WM481_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079933.1"
                     /translation="MRFKAEYARSKNDADDKRGNAHIANGAIGKMAAVYAGYTYTQPG
                     GESTRFRGGLERVQVKRYLGEKCCGYACPKSERFTLQADGEIRQNLGEGWFLKL"
     gene            37551..37790
                     /locus_tag="WM481_000550"
     CDS             37551..37790
                     /locus_tag="WM481_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079934.1"
                     /translation="MSGVAVYLRNKNKIAIASQDANLNSKGRFVSSGLNVGKQLTGSL
                     GVEFDPYYRHRAIRKSAEFVPNTTKTKTDSEKFNE"
BASE COUNT         9084 a        10847 c         9404 g         8736 t
ORIGIN      
        1 gggcgacgga aaggcggcgc gggggaaggg tgtgtgtatt gtcgttcatt gtcgatattt
       61 catcattgcg gactcaattt caatccggaa caaccttcat ttttggggaa atccgcgtca
      121 tgaaagagtc cagcagtatg gttaccggcc cgtcgttgca gagcgacacc tgcatatgcg
      181 tgcggaaacg ccctgtttcg acatgaatcc cgtgtccgcg taacagctcc gccgttcgca
      241 ggtaaagccg ttgcgcctgt tctgccggcg cggcttggga aaacgaaggc cgccgcccgc
      301 ttgccgcgtc ggcataaagc gtaaactgcg acaccagcag caccgcgccg ccgacatctt
      361 tcaaagacag gttcagcttg cccgcttcgt cttcaaacac gcgcaaatgt gcgattttgt
      421 cggcgatata gcgtgcatct ttttctgtgt cgctatgcgt tacaccgagt aacacgacaa
      481 acccgccgtc gattttgccg caggtttccg tgccggcttc ggacacgaca tccacctttg
      541 cacctaccgt tttctgtatg accgcacgca taagccgtcc tccttacctg aacgtgcaat
      601 gccgtctgaa aaggcttcag acggcatcgg tatcggaaat gccattacgg tttgacaccg
      661 ttttcttcca aaagggcatc gattttcaag cgggcttttt cctgcctcaa accttgttcc
      721 aactggtttc tgaccaactc gaaaggctgc gcgtcggggt ttttcccgac cgcgccgagt
      781 ttgaacaggt aatagcgttc gcccaatttg accggattgc gggtaacgtc gccacggttc
      841 ataccggcaa actgcgaagc cagcggctcg ggaagctgct gcgccataat gaaaccgtcg
      901 aacgcctgct cgtcgttcgg ataacgcttc atcagccctt caaaagacag ccctttgagc
      961 aggagctgct gcgcctgacg cgcctcctct tcggttgcga agctgacctg ctgcaatttg
     1021 atcatgcgga tttgccgctc ataaaactga cgcagtgcgc tttcggaaac cgtttccgaa
     1081 cgttccagaa aacggacgta ctcctcggca taaaaagacg cttcggcgat tttaaagcgg
     1141 ttttggacat ccttatcctt atccaaacct tccttcaatg ccctgttttt caaaacttcc
     1201 aaagtttgca gccggcggac ggcatcgttt cggattgcct gcccgtccgg tctttgggac
     1261 tgctccgcat gccggtctgc ctgctgcatg atctgcgcca ccagcgtatc caccaaagcc
     1321 gggtcgattt cgggtgcttt ggctgccgca aaacctgcca acattgcagc aataactgcg
     1381 gcagcggtct ttttctgttt catggtattc cctgtcctgc gcggcggcaa aacgcctgta
     1441 tgaacgtctt gccgccgtat cccgttttca gattattttg caggtttgat gtttgcctta
     1501 cccaacagcg caccgacggc acggtcaatc cgttccgcct gaaggttgcc ggcaatctgt
     1561 cctttcattt cgtcaaaaga aggcactttc acctcgcggc tgtcgttgac ataataaacg
     1621 ccgtagaaat cgccgttttt cagcggcgtt gccgtaaatt cgcctttttt caagtcctta
     1681 attgcctgat aaagcggcgg aacaccctgt tccaaatctt tcagcggcac atatccgtcc
     1741 ggcgcgccgg tccgtttggt gcggtcgttg agcgagtatt gtttcaaaac ggcatcaaaa
     1801 ccttttttcg ccttcaaatc ggcaaccgct tttttcgcat tttcttcctt gtcggtcagg
     1861 atttcgccca actggacttc ctgcgtgcct ttataaaaac cgctgatatt gtcgtaaacg
     1921 gcttttactt cctgctcgga aaccggttgg gttttggcga tatgcagtgc gtatgcctcg
     1981 ccgttcaagc catattttac cgcctgccaa acggttttga aggacggttt cttgtcgtcg
     2041 cccgactttt tcgcttcggc acgcaatttg gcaagcgcat ctttaaactc tgccgaccgg
     2101 tcgagtttca ggcgtttcac ttcctgtgcg accacggtgt tgaccacttc gttttccagc
     2161 agggattggc gcagttgcgg cgtgtcttcg gcacggctgt tttccgcacg gaatgcggca
     2221 acctgcgcat cgatgacgga actgtcgatt ttctgaccgt taacggttgc cagcgtttgg
     2281 gcaaacaggc tgccggaaca ggcaagcagc gcaacggaag tcaggatttt tgctttcata
     2341 atcttctctc tttaaatttt aaattgaaat attcaggcag acttcattca gtccgccgta
     2401 tggaaatact cgtcaggggt agccgcccgg atgccgagcg cgtgaatcat gccgcctgaa
     2461 aataaatctt tgagcagcga tttgaccgtt ttctgacggt tcagacggct taaaccttca
     2521 aaacggccgc tgacgaccaa aacggcataa tgtccgccgc cggtattgcc cgcgtgtcct
     2581 ttgtgcagat ggctctcatc gccgatttcc aacaccagag gatcgagcgt ctgcaggcgt
     2641 tcgcggatca aatcgacagc cggcattatt tgaacaccgc tttaaacggc ttgatcagca
     2701 cttcgctgta cacgccggca tgaacatagg gatcgtcttc agcccaagcc tgcgccgcat
     2761 ccaaagactc gaactgtgcc acaatcaagc tgcccgaaac acgttcggga ttgtccggca
     2821 gcaggtttgg gcctgccgtc agcagacggc cttccgattt cagcgtttcc agccgtttaa
     2881 agtgttcggg acgtgccgcc atacgcgctt catgcacatc ctccccgtct gttgccagca
     2941 acataaaata ttccacagtc aatcctcctt tttcagatag gtactcagat aaataccctg
     3001 aataataaag aaaaaaagca tcagcgcagt cgaaccgaac atcttatagt taacccattg
     3061 cgcttcaaac ctagtaaaca caaaccagtt ggcaataccc ataaaaatca gaaaaccgac
     3121 ccacatatat gtcaattttc cccatacggc atccggaagc tgaatctccc tgccgatact
     3181 cgctttcaag ccgtttttac ccgcaaggtg gctgcccagc aggaataacg ccccgcacca
     3241 gaacaatact gtcggcttcc acataatgaa gcggctgtcg cccaaaacaa tggttgcgcc
     3301 gccgaatacg acaatcagca ccagtccgac ccactgcatc gtatccagcc ttttatgctt
     3361 ccaatacagg aaagccgcct gaaccacgcc tgcaaccaag gcaaccgccg cagcggcaat
     3421 catatttttg gtaacggtat aagtagcaaa aaacaagatg acggacaaaa ggtcgctgac
     3481 aaatttcata tccaaatgag aaagcaaatc aaataaacgc taatcataca ggcagaaccc
     3541 tatctttgca cctgcaaaac caacaaattt tgttttgatt aatgcaaata tgcacagatg
     3601 tttttgaaaa aagatggaaa tatgtcataa tttataaaat tggctttaaa atacagcttc
     3661 ggtcggaacc tctgcctatc cgcaccctat cgtccgtatt gcctatgcct gatgacgaaa
     3721 tttacagact gtacgcggtc aaaccgtatt cagccgccaa cccacagggg atacatcttg
     3781 caaaaacaac agacaaatca aactgattgc cgcctccatc gcagttgccg catcctttca
     3841 ggcacatgcg cgactgggcg gactgaatat ccggtccaac cttgacgaac ccttttccgg
     3901 cagcattacc gtaaccggcg aagaagccaa agccctgcta ggcggcggca gcgttaccgt
     3961 ttccgaaaaa ggcctgaccg ccaaagtcca caagttggac gataaagccg tcattgccgt
     4021 ttcttccgca caggcagtcc gcgatcccgt cttggtgttc cgcatcggtg caggcgcgca
     4081 ggtacgcgaa tacaccgcca tcctcgatcc tgtcggctgc tcgcccaaaa ccaaatctgc
     4141 actttcagac ggcaagacac accgcaaaac cgctccgaaa gcagagtccc aagaaaatca
     4201 aaacgccaaa gccctccgca aaaccgataa aaaagacagc gcaaactcag ccgtcaaacc
     4261 ggcgcataac ggcaaaaccc ataccgtccg caaaggcgaa acgctcaaac agattgccgc
     4321 cgccatccgt ccgaaacacc tgacgctcga acaggttgcc gatgcgctgc tgaaggcaaa
     4381 cccaaatgtt tccgcacacg gcagactgcg tgcgggcagc gtgcttcaca ttccgaatct
     4441 gaacaggatc aaagcggcag cacccaaaaa aatcaaagcg gaacaaccca aaccgcaaac
     4501 ggcgaaaccc aaagccgaaa ctgcatccat gccgtctgaa ccgtccaaac aggcaacggt
     4561 agagaaaccg atagagaaac ctgtcgaaaa accggttgaa aaacctgaag caaaagttgc
     4621 cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc gaacccaaac ccgcagtgtc
     4681 cgaaactccg gcttcggcga cagaacgcca acccggacct gtacccgctg caaatactgc
     4741 cgcatcggaa accgctgccg aatccgcccc cccaagaagc cgccgcttct gccatcgaca
     4801 cgccgaccga cgaaaccggt aacaccgttt ccgaacctgt tgcggaaaca gccagtgaag
     4861 ttgccgccga acccgttgcc gaacctgtcg aacaggtttc tgccaaagaa gaaaccgaaa
     4921 gcggactgtt tgacggtctg ttcggcggtt cgtacacctt gctgcttgcc ggcggaggca
     4981 cggcattgat cgccctgctg ctgcttttac gccttgccca atccaaacgc gcgcgccgta
     5041 ccgaagaatc cgtccctgaa gaagagcctg accttgacga cgcggcagac gacggcataa
     5101 aaatcacctt tgccgaagtc gaaactccgg caacgcccga acccgctccg aaaaacgatg
     5161 taaacgacac acttgcctta ggtggggaat ctgaagaaga gttatcggca aaacaaacgt
     5221 tcgatgtcga aaccgatacg ccttccaacc gcatcgactt ggatttcgac agcctggcag
     5281 ccgcgcaaaa cggcattttg tccggcgcac ttacgcagga tgaagaaacc caaaaacgcg
     5341 cggatgccga ttggaacgcc atcgaatcca cagacagcgt gtacgagcct gagaccttca
     5401 acccgtacaa ccctgtcgaa atcgtcatcg acacgcccga accggaatct gtcgcccaaa
     5461 ctgccgaaaa caaaccggaa accgtcgata ccgatttcta caacaacctg ttctcaaaca
     5521 accatatcgg cacagaagaa acagcttccg caaaacctgc cgcaccctcc ggactggcag
     5581 gcttcctgaa ggcttcctcg cccgaaacca tcttggaaaa aacagttgcc gaagtccaaa
     5641 caccggaaga gttgcacgat ttcctgaaag tgtacgaaac cggtgccgtc gcggaaactg
     5701 cgcctgaaac gcccgatttc aacgccgccg cagacgattt gtccgcattg cttcaacctg
     5761 ccgaagcacc ggccgttgag gaaaatgcag cggaaatcac tttggaaacg cctgattcca
     5821 acacctctga ggcagacgct ttgcccgact tcctgaaaga cggcgaggag gaaacggtag
     5881 attggagcat ctacctctcg gaagaaaata tcccaaataa tgcagatacc ggtttccctt
     5941 cggaatctgt aggttctgac gcgccttccg aagcgaaata cgaccttgcc gaaatgcatc
     6001 tcgaaatcgg cgaccgcgat gccgctgccg agacagtgca gaaattgctg gaagaagcgg
     6061 aaggcgacgt actcaaacgt gcccaagcat tggcgcagga attgggtatt tgattcccaa
     6121 ctgccctttc gcagatcaag gatgccgttt cagacggcat ctttttttgc cttatcggtg
     6181 taacggataa agtttgaacc ggcacaggct caaacagcag gccgacggca acaaaatgcc
     6241 gtccgaaacc cctaaaggct tcagacggca ttggcggcgg cggtatcagg tattcgcata
     6301 ggcaaccgaa tcgtcgccgc ttttcttttg aacgaactcg attttgcatc cgtcggggtc
     6361 ttctacgaag gctatcacgg ttgtgccgtg tttcatcagg ccggcttcgc ggacgacgtt
     6421 tccgccctgc cgcttcacgc gttcgcaggc ttcgtaggca tcgtccactt caaccgcgat
     6481 gtgtccgtag gcgtcgccca agtcgtatcg ttccgtatcc cagttgtgcg tcagttccaa
     6541 aaccgtgctg tcggtttcgt cgccgtaacc gacgaaggca agggtaaatc tgccttcggg
     6601 atagtctttt cggcggagca gtttcatacc caaaacgttt tggtaggaat cgagggattt
     6661 ttcgagattg cccacgcgga gcatagtatg gagtaagcgc atttttcgtg ttcctctcgg
     6721 tggtggttaa acttcgattt tattcggcgt aaacgtctgc catttgttgc aggcgggaca
     6781 gtgccagaaa aagacttggg atttgaagtg gcagttgcgg caacggtaca tcacgctgcg
     6841 ctggagctgc cgtccgataa ccgaacgcat catgtcggca tcggctttcc aagccggatc
     6901 caaatcgctg agtttcaaac cgagcaggcg gtacacgccg ttaaggtcgg gcttgcggcg
     6961 gacaagctcg acggcggttt gcgcggcttc tttctcgccc ttaagcagca gggatttctc
     7021 gtacacgaca ttgatcaggt caagttcggg aaacgtctgc atatatcctg tcagacggtt
     7081 caagccttct tcaggttttc cctgcgcggc ataggcttcg taaagcttct cgccgaccat
     7141 gctcaagtat gcatggtttt gctgctcgat ggcggcatag gcttcgacgg cggcagggaa
     7201 attgccttgt cggtgttcaa tgtcgcccaa aatcatgttg gcgcgggtgc attttttgtt
     7261 ggcttcgagt gccttgccga cattgaaacg cgcggcatcg aaattggact tgaacagcgc
     7321 ggcttgggca agttcgcaat aaaactgtgc aatctcaaac tgatatgtct gttcgtcgtg
     7381 actaagaagt tgggcggttt caaccgcttt ttcccaatcc ctgtcctgct ggtagatatt
     7441 gagcaggtgc tgtctggctt cacgcgccat ttcaccgtct tgcagcccca aaaaaatctg
     7501 ttcggcacga tcgaccaaac ccgcgctttg gtagttttgc gccaattcaa acaggacgcg
     7561 cgcgcgcttt tcgccgaccg tatcgggaga atcgagcatt gtccggtgta tgttgatggc
     7621 tttgtcgttt tcgccgcgct gacggtaaag tttgccgagg gtaaggttca aatcatacga
     7681 ttgcggccgg ccgtcgacga cttccgccaa ctcccttgcc gcgcgcccgc tgttgcggtc
     7741 gaccaaagcg tccaggcttt tataaaatcc cgaagggatg ctttttgcct gcttcaatac
     7801 ggttttcata tccacgcggg cggcaaacca gcccatcgtg aagaagacgg gcaaaaggat
     7861 aatcggcagc aggataatcc acaattcgtt gtccatatcg gctttcttaa ggctgtttgg
     7921 cagattcggc agcattttgt atcggcggtg cagtcaattt ctgtccgctc aagcgcgcac
     7981 ttttcttcac ttccgcacgc aggcggctgt tttcgccgcg caaggacagc agccgcccga
     8041 acagggcaaa cattccgaac acgatgccga cgacaaacgc gccgaacaat acgacaatca
     8101 gcggcagatt gacactctgc cccggaagat aggaaaaggt aacggcatcc atattaatga
     8161 cggcaagcag caggaagagc agcaggataa tgattttgat gacggtatag ataagtttca
     8221 tgtttgctcc gtaaataaaa acgccgcacg gacagcttcg cactctgaac caagattgcg
     8281 ttagtttaaa cgatttgcac ggtttcggat agaatgcggc agcgtgttcg gacggcatac
     8341 ggagtatgcg attgccgaca attttaccaa atacaccgct cctttccatt tgaaaaataa
     8401 cggattggac accgcatcga cagaaaaacc cgccgcgcac ttgtcaaaac cctgtttgca
     8461 ggcgtatctt tacaaccttc aaattcaaac cgttcattga aacatatcag aataagaaag
     8521 gctttacatc atgagcagac ccgtacccgc cgtattcggc agcgtttttc acagtcaaat
     8581 gcccgtcctc gcctaccgcg aaggcaaatg gcagccgacc gaatggcaat cttcccaaga
     8641 ccttaccctt gcacccggcg cgcatgccct gcattacggc agcgaatgct ttgaaggttt
     8701 gaaggccttc cgtcaggcag acggtaaaat cgtgctgttc cgtccgaccg ccaatatcgc
     8761 gcgtatgcgg caaagtgcgg acattttgca cctgccgcgc cccgaaaccc aagcttatct
     8821 cgatgctttg gtcgaactgg tcaaacgtgc cgccgacgaa attcccgatg cgcctgccgc
     8881 gctgtacctg cgcccgacct taatcggtac cgatcccgtt atcggcaagg ccggttctcc
     8941 ttccgaaacc gccctgctgt atattttggc ttcccccgtc ggcgactatt tcaaagtcgg
     9001 ctcgcccgtg aaaatcttgg tggaaaccga acacatccgc tgcgccccgc atatgggccg
     9061 cgtcaaatgc ggcggcaact acgcttccgc catgcactgg ttgctgaagg cgaaagccga
     9121 atacggcgca aatcaagtcc tgttctgccc gaacggcgac gttcaggaaa ccggcgcatc
     9181 caactttatc ctgattaacg gcgatgaaat cattaccaaa ccgttgacag acgaattcct
     9241 gcacggcgtt acccgcgatt ccgtgctgac tgtcgccaaa gatttgggct ataccgtcag
     9301 cgaacgtaat ttcacggttg acgaactgaa agctgctgtg gaaaacggtg cggaagcgat
     9361 tttgaccggt acggccgccg tcatctcgcc cgttacttcc ttcgtcatcg gcggcaaaga
     9421 aatcgaagtg aaaaaccaag aacgcggcta tgccatccgt aaggcgatta ccgacatcca
     9481 gtacggtttg gcggaagaca aatacggctg gctggtcgaa gtgtgctgat gctttaaata
     9541 aaaaaatgcc gtctgaaacc cgtttggcgt ttcggacggc attttcgcgt tcggaccgtt
     9601 tccgccgcac ctgcagcaag tcggtacaaa ggcaatcggt taaaacaagc gtccgcattt
     9661 cccatcccgc ctgccgcaag tcgggcattt ccctggaaat acgcttcaaa cggcaaaccg
     9721 ccgcccgaaa ccgatactcg gcacggacgg cgcacggatt tgaaaacggc ggattatccc
     9781 tcggtgctca aggcattgat gctgtaaccg ccgtcaacat aagtgatttc gccggtaatg
     9841 ccggacgaca ggtcggacag caggaaggcg gcagtattcc cgacttcttc gatggtaacg
     9901 ttgcggcgca gcgggttgtg ggcggcgacg tgtcccaaga gtttgccgaa atcggcgatg
     9961 ccggaggcgg cgagcgtttt gatcgggccg gcggaaatgc cgttgcagcg gatgccctct
    10021 ttacccagac aggcggcggt aaagcggatg cctgcctcaa ggctggcttt tgccataccc
    10081 atcacgttgt aattcggaat cgcgcgtacc gcgcccaagt agctaagggc gacgatggcg
    10141 gagtttctgc cgcgcatcat cggacgggcg gcttttgcca atgcgggcag gctgtatgcg
    10201 gaaatttcgt gtgcggtgtt gaacgcttcg cggctgatgc tgtcgaggaa gtcgccgctc
    10261 aaggcttctt tcggcgcgaa gccgatggaa tgcaccaaac cgtccaagcc atcccaatgt
    10321 ttgcctaagt cggcgaacac ttggttgatt tcgtcgtcgc tggcgacatc gcagcggaat
    10381 acgagttcgg aatctaattc cgccgccatt ttgcggacgc gctcttccag tttgtccaca
    10441 acgtaggtaa acgccagttc cgcgccctgt tcgcggcagg ctttggcgat gccgtaagcg
    10501 atggaacgct cggaaatcat gccggtaatc agaatttttt tgccttgcag aaaacccatt
    10561 ttgtatcctt caaatagtgt tcggttcgtt ctaaaccgtt gattatagca aattgtccct
    10621 gtttctgtgt tttcacgttg cagcgtgcag acggcaatgc cgtccgaagc ggatttcaga
    10681 cggcattgga cgtttcaaat acggtttaag gcatcagatg ccgcgcaaga gttcgttcac
    10741 gctggttttg gcgcgggttt gcgcgtccac gcgtttgacg atgacggcac agtaaaggct
    10801 gtggctgccg tctttggaag gcaggctgcc ggacacgacg accgaacctg ccggtacgcg
    10861 gccttggtag atttcgcctg tggtgcggtc gaagattttg gtggattgac cgatgaacac
    10921 gcccatcgaa atcacgctgc cttcttcgac aatcgcgcct tcgacgattt cggaacgcgc
    10981 gccgatgaag cagttgtctt caatgatggt gggtgcggcc tgcaggggct cgagtacgcc
    11041 gccgatgccg acaccgccgc tcaagtggac gtttttgccg atttgcgcgc aagagccgac
    11101 ggtcgcccaa gtatcgacca tcgcgccttc gtcgacgtat gcgccgatgt tgacgtaaga
    11161 cggcatcagc acggcgtttt tggcaacaaa gctgccgcgt cgggcaaccg cacccggaac
    11221 tgcgcggaag cctgcgtttt tgaactcgtc ttcagaccag tcggcaaatt tggtcggcac
    11281 tttgtcgaag tatttgttca cgccgtcgtt gaggacttcg ttgtcttgga tgcggaagga
    11341 cagcaacacg gcttttttcg cccattcgtt gactttccac tcgcccacgc ccaagcgttc
    11401 ggcgacgcgc agtttgcctg aatcgagttg gcggatggtt tccaacacgg cttctttgac
    11461 ttcgggggca acggtggtcg gagtgatgtc cgcgcggttt tcaaaggcgg tttcgataat
    11521 gttttgcaaa gacataatgt ttccttggtt cgagatattt cgggacgctt gccggtattg
    11581 ttcagacggc atggcggttg gaacaaacgt gctattgtgc catacaaacg cccgcccgtc
    11641 agccgcgccg caggcttgcc ggatgccggc gtttcggaat cgggcgggcg gcaggcatat
    11701 gtctttttcc gcgcccgaat caatacagaa aggcggcggt acttttatgc cgcgccgccg
    11761 cctttcagac ggcattcgcg gtaaaacgcc atcagccctt cggtcgaggc atcgtgtacg
    11821 gacgtgccgc cttccagttc gccgatgatg gtttttgcca actgtttgcc gtactcaacc
    11881 ccccactgat cgaaggggtt gacgttccat atcgcgcctt ggacgaaggt tttgtgttcg
    11941 taagccgcca tcagcatacc cagattgcag ggcgtgaggc ggtcgagcag gatgctgttg
    12001 ctggggcggt tgccggggaa ctctttgtgc ggcgcgaggc gttcgcgttc cgcttcgggc
    12061 aaatctgcca gttcggcgcg tgcttcgtct aaggttttac ccttcatcag ggcttccgct
    12121 tgggcaaagg cgttggcaac ggtaaaacgg ctgcgtccgt cctccgcgcc ctgcgccgtc
    12181 atcgggacga taaagtcgca gggaatcagg cgcgtgcctt ggtggagcag ttggaaatag
    12241 gcgtgctggc agttgacccc ttcaccgccg aacacgatgc cgcccgtttt gcacgcggcg
    12301 ggactgccgt ctgaagcgcg gcttttgccc aaactctcca tatcgagctg gttcagccac
    12361 gccggcagca ggcgcaggtt gtggctgtac ggaacggcgg tctgcccgtc cgcgtgctgg
    12421 aaattgttat accacacggc aatcagtgcc atcagaacgg gaatgttgcg acgcgggggc
    12481 gtgtggaaaa aatggctgtc catcgcgtgc gcccccgcta gcagttcgcg gaaacgcgcc
    12541 ccgccaaccg caaccatcac gggcaaaccg acgggcgacc agacggaata gcgtccgccc
    12601 acccagtcgt acatcgcaaa cacgcgttcc gccgcaatgc cgaaactttg cgccgcttcc
    12661 gtatcggcag acaccgcgca aaaatggcgc gccgtttcgg attccgagaa ccccgcaccg
    12721 cgataccacg ccttgaccgc ctctgcattg agcagggttt ccggtgtttt aaaggacttg
    12781 ctggcaacgc aaaacattgt tgtttcgggg ttcagacggc ataagacctc atccaggcag
    12841 gcagggtcgg cattagaaac aaaatggacg gaaatctgcc gtctgaacgg ctcgagcgcc
    12901 tgcacgcaca ttgccggccc gaggtcggat ccgcctatgc cgatgtggac aaaatccgca
    12961 atccgttttc cggttatccc ctgatataaa ccgtcgtcca aactgtgtgc aaacttcaac
    13021 gcacgattta actcgcggcg gatttcgggc aacacgtccc tgccgtccgc ataaacggca
    13081 tccgcaccgt cgggcaggcg caaagccgta tgcagcgcgg cacgcccctc gctgccgttg
    13141 actttcgcac ccgtccgcaa agcacgcatt ttcccttcca aatccgccgt ctccgcaaga
    13201 cggcagagca gttgcagcgt atcttcgcca aaacggtttt tgctgtaatc gaacaacatc
    13261 ccgtccaaac gctcatgcat acgctcaaaa cggtcgggtt cggcagcaaa gcggtcgcgc
    13321 aaaaggatgt ggcacgtatc ctgataatgg cgttcgagcg cataccatgc acgggtaaag
    13381 gcatccatct gttttccttg atttttcaga accggattaa aatatagcag aatgtagtct
    13441 aacaaacggc agacacttta gcgaatctca ggaacaccgc ccgccgcgtc caataacacc
    13501 ttatttccat atggcgaaaa tatagtgaat taacaaaaat caggacaagg cggcgggccg
    13561 caggcagtac ggatagtacg gaaccggttc gcttggtgct tcatcacctt agggaatcgt
    13621 cctctttgag ccggggcggg ccaacgccgt accggttttt gttaattcac tataccctcc
    13681 ggccggcatt tgacccgcaa caatatcctg caagatttat tgtgtgcgca taaatgccgg
    13741 acagccgcct aaattaaggt ataatcgcat ccgattctgt cccgtttgcc gcccgcaggc
    13801 gggatgtcgg cggttttcat cttaccggga acacgccgat actgtttttt aacgatgttt
    13861 cttacatttt attccaatta ctttacgggg ctagaatatg gctaaaaatg ggggattttc
    13921 tttgttcgca aagaaagaaa aacgctttat ctttgaaggc aggcattctg cctccgacaa
    13981 actggtcaac ggcgaagtat ccgcgtttac cgaagaagag gcacgcaaaa aactggcaaa
    14041 acgcggcatc cgcccgttgc agattacccg tgtgaaaaca agctccaagc gcaaaatcac
    14101 acaagaagac attaccgttt tcacccgcca gctttccacg atgattaaag cgggcctgcc
    14161 gctgatgcag gcatttgaaa tcgtggcgcg cggacacggc aacccgtcta tgacggaaat
    14221 gctgatggaa atccgaggcc aagtggaaca gggcagctcg ttgagccgcg cattctcaaa
    14281 ccacccgaaa tatttcgacc gcttctactg caacctggtt gcggcgggcg aaacgggcgg
    14341 tgtattggaa agcctgctgg acaaattggc aatttacaag gaaaaaaccc aggccatccg
    14401 caaaaaggta aaaaccgcgc taacctatcc ggtatccgtc atcgccgtcg ccatcggttt
    14461 ggtattcgtg atgatgattt tcgtactgcc cgcctttaaa gaagtttacg ccaatatggg
    14521 cgcggagctt cccccactga cccaaacagt gatggatatg tccgactttt tcgtctcata
    14581 cggctggatg gtgctgatcg cactgggctt tgccatatac ggcttcctta aattgaaggc
    14641 gcgttcgatt aaaatccaac ggcgtatgga tgccatactg ctgcgtatgc cgattttcgg
    14701 agacattgtc cgcaaaggga cgattgcccg ctggggcagg acgacggcaa cgctgtttgc
    14761 cgccggcgtg cctttggtcg atgtattgga ctccactgcc ggcgcggcgg gcaatttaat
    14821 ctatgaagaa gccacccggg aaatccgtac gcgcgtcatc caaggtctgt ctatgacttc
    14881 ggggatgcgt gcgacggaac tgttccccaa tatgatgttg cagatgtcct ccatcggcga
    14941 ggaatcgggt tctttggacg atatgctcaa caaagccgcc gaattttacg aagacgaggt
    15001 ggacaatgcg gtcggcaggc tgtccgctat gatggagccg attattattg tgattttggg
    15061 cttggtcatc ggtacgcttc tggtcgctat gtatctgccg ctgttcaact tgggcaacgt
    15121 ggtcgcctga tttgccgcac agatccggcg cggattggtt ctgcgccggt ttgtttttgc
    15181 tttgaatata tcaaggacaa aatatgtctg atttgtctgt attgtcgccg tttgccgtgc
    15241 ctttggcagc agttttgggg ctgctggtcg gcagcttcct gaatgtcgtc atttaccgcg
    15301 tacccgttat gatggaacgc ggctggacgg tatttgccaa agaacattta aacctgccgc
    15361 tgaccgacga tgaaagccgt accttcaacc tgatgaagcc ggattcctgc tgtcccaaat
    15421 gccgtgtgcc gatacgcgcg tggcagaaca tcccgattgt cagttacctg ctcctgcgcg
    15481 gcaaatgcgc ttcctgccaa accaaaatca gcatacgtta tcccttaatc gagctgctga
    15541 ccggcgtatt gttcgggctg gtcgcctggc aatacggctg gtcttggatt acgctgggcg
    15601 gtttgatact gaccgcgttt ctgatttccc tgacctttat cgatgcggac acccaatacc
    15661 tgcccgactc gatgacatta cccttgatct ggctggggct gatatttaat ttggacggcg
    15721 gcttcgtgcc tttgcagtct gccgttttag gtgcggttgc cggctatagt tcattatggc
    15781 tcttatgtgc agtgtataaa ctgctcacag gaaaaaccgg tatgggcaac ggagatttca
    15841 aactgattgc cgcattgggc gcgtggctcg gcatatccgc attgcccgtg ctgatttttg
    15901 tttcctctct gatcggtttg gtcgcggcaa tcgttatgcg cgtcgccaag gggcggcatt
    15961 ttgccttcgg ccccgcactg acagtttcgg gctggataat ttttacggca aacgattccg
    16021 tatggcgggc ggtcaactgg tggctgaccc atccggtgag atgacagcat gggtcggact
    16081 gaccggcgga atcggcagcg gcaaatcggc agccgcgcaa tattttgccg atttgggcgt
    16141 gccgcgcatc gatgcggacg cggcggcgca ctcgctgacg gcttcagacg gcatcgccct
    16201 gccggaaatc aggcggctgt tcggcgacac cgttttcgac acacagggtt tgttgcggcg
    16261 cgacatattg cgtaaagaaa tctttgcctc cccatcgcgc aaagccttgc tcgaatccgt
    16321 gatgttgccg ctgattttct cagaaatcaa aaaacagcaa gaaaccttta ccgatgcggt
    16381 ttacggcatt gtcgaaattc cgctgctgac ggagaagcgt caatttatca gcctgatacg
    16441 gcgtgtcctg accataagcg cacctttgga aaaacgtatc ggcagggtaa tggcacgcag
    16501 cgggctgacg cgcggcgagg tcgcggacat catcagccat caggcatccg aatccgaacg
    16561 cctgctgctt gcagacgatg tactgctcaa tgacggcagc ctcaaaagcc tacgtgagaa
    16621 aacaatgctc ctgcacgcgt tttattcagg gattttcgcc tcaaaaccaa cacaaggaaa
    16681 acacaatggc tgaatcgcgg caaacacgcc ttcaagtcaa atgtccgacc tgtcaaacgg
    16741 cagtagtatg gaaaccggaa aacgcattcc gcccgttctg ttcgcaacgc tgcaaactga
    16801 tcgacttggg cggatgggca gacgggaaat atacggtttc cggccaaacg gaaagtttgc
    16861 cggaaatatc cgaacccgac ggggcatacc gctgaccgcc ccgccttccc ggcaaacacc
    16921 ctgaaaggtc aaatgccgtc tgaaacaaac acgcttcaga cggcattttc attctcaaac
    16981 ctaatcgttg gtatttgccg ttacctcttc caatgaagta atgccctgca taattttcaa
    17041 aataccggcc cggcgcaaat ccaccatacc ctccttatag gcaacgtcca aaatacccac
    17101 ttccgtaccg ttgttcataa tcacacgctg catttcttcg ctgatgggca taacctcata
    17161 cacgcccgca cgccccttat aaccctgccc ccggcaacgg tcgcaaccga cggcgcggta
    17221 aagtttccaa tcttttgcaa gatcctcatc ggtgaaaccg acttccttca aagcagaggc
    17281 agacgggcgt tccacttcct gtttgcagct cgaacacagc ctgcgtaaaa gacgctgcgc
    17341 cataatcagg ctgaccgaac tggcaatatt aaacggcgcg acacccatat tcagcatacg
    17401 cgacaacgtc gccggcgcat tattcgtgtg cagtgtggaa aacaccatat gccctgtttg
    17461 tgccgcctta atcgcaatat cggcagtttc caaatcacga atctcaccga ccataatgat
    17521 gtccgggtcc tgacgcagga aagacttcaa agcagcggca aaagtcagac cctgcttatc
    17581 attgacgtta acctgattga tgcccggcag gttaatctcg gcagggtctt ccgccgttgc
    17641 aatatttacc gactccgtat tcaaaatatt caaacaggta tagagcgaca ccgtcttacc
    17701 cgaacccgtc gggccggtta ccagcaccat cccgtaagga cggtgaatcg cttccaacaa
    17761 caattttttc tggaacggct caaaaccgag ctggtcgatg ttcaaagacg cggcatcgga
    17821 attcaaaatc cgcatcacga ccttttcgcc aaacagcgtc ggcaatgtgc tgacacggaa
    17881 atcgacaggc ttgccgccct tttgaaaggt cagctgcatc ctaccgtcct gcggtatccg
    17941 tttttcggaa atgtccaaac gcgacattac cttaatccgg gaagcaagct gcccccttac
    18001 cgcaatgggc ggctgaacca cctcgcggag ctgcccgtcc acacggaaac ggatacgcgc
    18061 attgtgttcg taaaactcga aatggatgtc ggatgccccg ctacgcaagg catccgacaa
    18121 agttttatgg ataaacctcg gaacagggcc gtcttctgcc tcctcgttgt cgatatacag
    18181 ggtgtggctt tcctcttcct cttgcccctc cccaagctcc tgaagcagcg atgtcgaacg
    18241 cgaacccacc caatcgagca aacccgccaa ctggtcatcc tcgacaatga ccaactcaac
    18301 cgcaatccct gcggcagaaa cggttttctg aatttgcggc atctgggtcg gatcggaaac
    18361 cgcaaaaaat actttgtcgc cccgacggaa aaccggcaca cagtggaact ccaccatctg
    18421 ctcctccgtc aacaccccca tcagcaccct gtggcgcgga taatgacgca aatcaagaat
    18481 cgaataactg aacaccctcg caatcaatgc cgcaagcgac ttgggcgaaa tgacaccgtc
    18541 tgaaaacagc atcggcaaca cttccttacc cgcctgcgac tcattgtagt aatgctcggc
    18601 ctgctcaaca gtaaccacct ggttttgaac cagaatcctc agcaaaccta cgctcatacg
    18661 accttatccc caaatttatt cattgttata cctgtacaga ttttatcata aaacaacaga
    18721 tttccaagcc tgaacatctt ttacgaagcc tgaaaatcat ttcacgattc tgccgtccta
    18781 aaggtcggtt tttcaagcag gaagaaaaag ttttcagatg gcaaaaaagc cctccagcac
    18841 tgaaaggcct tatatcggaa acttcccgca acacgggaaa cagacaaatg caaaccgtca
    18901 aacctcgcca acaggaatcg aacctgtatt ttacgcttag gaggcatacg ttctatccgt
    18961 tgaactatgg cgagccgaaa tgaaaaggga gattttaaac ctttccggca acaaaagaca
    19021 agatgtcggc cgcaatcagc ggcccgacag ggataccgcc cataaatgcc accccgatga
    19081 ccgtaccgat cagcaagccc gttaccaata ccggctgccg tcccatcaaa ggcacgccgc
    19141 atcccgccag ccaagccacg aaaataccga taaaaacggc ggatatcatt ttaaaattca
    19201 aaaattcggc aacaggagga acctgcgcct ttcctgaaac caacggactc aaaaccccta
    19261 tggtcaaaag aatgataccg aggttcaacc cgtgcttctc gaccaacggg acaaactgta
    19321 ccaatgccgt ctgctgcatc agcagcaata tggttgccga gacggtaatc gaattgttgt
    19381 tgctgaccac ccccaacaga atcagcgtaa ccagaaacag gggaacaaaa ctgaaattca
    19441 tcggttacga caccctgacc aaagattcct tcgccagctg aatcatagca tccttcactt
    19501 cgctgtcgga cagggcatcc aacgcggcaa cggcacaatc gactgctttg cgcgcctcgc
    19561 ctatcgaata tgccaaagca tccgaacgga cgacataatc gcggattttt tcaaaatagc
    19621 cgcgatctgc attttccaaa gcagtacgca catcgttcgc aacctgttcg gaaccctgac
    19681 gcatcagata aatcaaaggc agggtcggtt ttccttccgc caaatcgtcg ccgacgtttt
    19741 tacctgtttc ttcggtttcc cccgaataat ccagcacatc gtcaataatc tggaatgccg
    19801 taccgacgta cataccgtag tctttcaagg cctgttcgtg cccgggggaa gctttgccca
    19861 aaattgcgcc gacttgagcg gcagcttcaa acaattttgc cgttttatat tggatgactc
    19921 ggatatattg ttcttcggta atgtccgtat tgccgatgtt catcagctgc atgacctctc
    19981 cctcggcaat gatgttggtc gcatccgcca tcacttccaa aacgcgcata ctgcccgagg
    20041 caaccatcag ttgaaatgca cgggtatata aaaagtcgcc aaccaacact gccgccgcat
    20101 tgccgaacag attgtttgcc gttgcccgcc cacggcgcaa atcgctttca tcgacgacat
    20161 cgtcgtgcag gagtgtggaa gtgtggataa actcgaccat cgctgccagc gaatacagtt
    20221 tctcgtcatc ataaccgacc gccttacccg ccaaaatcgt cataatcgga cgcaggcgtt
    20281 tgccgcccgc gctgatgata tatgtaccga tttgcgaaat cagtgcgaca tcggattgca
    20341 ccgcacggtt gatgacttca ttgactttgg caaggtcttc aggcagatgt cgctggaaat
    20401 agggcagatt ctcgagcatg gcagactctc aggtttacgt ttccgtcaaa cggcagatac
    20461 cgcttgaaag ctgattcaaa tagcgggcaa ttatatcagc aaagccatca gggtacatct
    20521 tttcagacgg catatgattg cccgaaccgc gcaaactttg acaaaacagg caaataacaa
    20581 tacaatgccc aatttcgcac aatggtgtgc ggatattaac cataaccctt atggagttga
    20641 gtatgtacgc ggtcgtaaaa accggcggca aacagtataa agtttccgtc ggcgaaaaat
    20701 tgaaagtaga acagatacca gcccaactcg acagccaaat cgaactgacc gaagttttga
    20761 tgattgctga cggcgaatct gtaaaagttg gcgcaccctt tatcgaaggt gcaaaagtaa
    20821 cggctaaagt agtggcacac ggtcgtggcg aaaaagtccg catcttcaaa atgcgccgcc
    20881 gcaaacacta ccaaaaacgc caaggccacc gccaaaattt cacccaaatc gaaatcgtgg
    20941 caatcgccta accttaaaac ttaggagcat tacaaatggc aagtaaaaaa gcaggcggca
    21001 gcacccgcaa cggtcgcgat tcagaagcca aacgcttggg cgttaaagcc tacggcaacg
    21061 agctgattcc cgcaggttcc atcatcgtac gccaacgcgg taccaaattc cacgcaggcg
    21121 acaacgtagg tatgggcaaa gaccacactt tgttcgccaa aattgacggt tatgtcgaat
    21181 tcaaaaccaa aggcgcgctg aaccgtaaaa ctgtcagcat ccgtccttac accggttctg
    21241 aagaataatt caaccgatac ctgaagccgc atcttttcac gatgcggctt tttacatacc
    21301 ccatacccga tttgatacac attaagctga aagtaaaaat ccgcatacac cctcccctgc
    21361 atatttcttc aacaacgggt tttgatataa tcgcctatct gttacagata gttcaaacgg
    21421 cattcaaacc cttacaaatg ccgtctgaaa tcatcggcat ccgcctatgc aaaggatatt
    21481 ttatgagttt acacagcgac atcctcgtcg tcggcgcagg cccggcgggt ttaagttttg
    21541 cagccgaact tgccggcagc ggtttgaaag ttactctgat cgaacgcagc ccactgactg
    21601 ttctgcaaaa tcctccctat gacggacgcg aaatcgccct gacccatttt tcccgcgaaa
    21661 tcatgcagcg tttgggtatg tgggacaaaa ttcccgaaaa cgaaatctac cccttgcgcg
    21721 atgccaaagt gctgaacgga cgttccgact accagctcca ctttccccaa ccgactgaag
    21781 cgcgcggcga gcctgccgac tgtttgggct atctgatttc caaccacaat atccgccgcg
    21841 ccgcctatga aatcgtatca caactcgaca atgtcagcat tctgaccgat acggccgtta
    21901 aagaagtcaa aacatctgat aatgaagcgc aagtcttttt ggaaaatgga gacattctaa
    21961 ccgcgcgtct ccttttggca gcagacagcc gtttctcaca aacccgccga caactcggta
    22021 tttcttcaga tatgcacgac tacagccgaa ccatgttcgt ctgccgcatg aaacataccc
    22081 tttccaacca acataccgct tacgaatgct tccattacgg acgcaccatc gcactgctgc
    22141 cactggaaaa gcgcctgacc aataccgtca ttacagtcga taccgataaa atcaacagcg
    22201 ttcaaaacct ttcgcccgaa gaattggcgg caagcgtaaa agaacagctc aagggcaggc
    22261 tcggcgacat ggaactggtc agcagcatcc accattaccc ccttgtcggc atgattgcca
    22321 aacgtttcta cggcaaacgc agcgcgctga tcggcgatgc ggctgtcggt atgcaccccg
    22381 taacggcaca cggcttcaac ctcgggcttt ccagcgcgga tattctggca aaactgatac
    22441 tcgaagccga acagcgcggt caagacatcg gcgcggcttc cttgttggaa aaatacagca
    22501 gcaaacatat gctgcacgcc catccgcttt atcacggcac caatatgatg ctgaaactgt
    22561 ttaccaacga aaccgcgccg gcaaaactgt tgcgcggttt ggtattacga gccggcaaca
    22621 acttcccgcc gctgaaaaag ctgattacca aacaactgac cggttgaaaa ctgccgaaat
    22681 gccgtctgaa acgtttcaga cggcattttt gtattatgaa ccgttttgcg gtttccatat
    22741 ccttggcaga cggtttgcag cagattcgca tacggcagat gtttcaagca gaaaggaaac
    22801 acaataaaaa taaaaaagcc tccgaacagt cggaggcttt gctttcaaac tgaaattatt
    22861 tcagtttggt ttctttgtac actacgtgtt tgcgggcaac cggatcgaat tttttgattt
    22921 ccagtttgcc gggcatagtg cgtttatttt tggtagtggt gtagaagtgg ccagtacctg
    22981 caccggattc cagtttgatt ttatcgcgca ttgcagtaat ccttaattaa atagtgttta
    23041 aattaagctt cgccgcgagc acgcaaatca gccaatacga catcaatgcc tactttgtcg
    23101 atggtacgca atgcagcgtt ggaaacgcgc aggcgaaccc agcggttttc actttctacc
    23161 caaaaacgac gtgattgcaa gttgggcaaa aaacggcgtt tggttttgtt gttggcgtgc
    23221 gatacgttgt tgccggacat cgggcgttta ccggtcactt tgcaaactcg tgccataatc
    23281 agtctccaaa cttctaaaat agtaaaacgc gatttatacc acgaaaaaca atgccgtttc
    23341 aagtgtttta aagaaaaaca gatgttttgc gcgggtaagc cgcccgagtg ccgcatctgc
    23401 ggaacgtttg caggtgcgaa acagatgggg cgcgattata gcggatttcg ggagcttctg
    23461 cattcaaaat gccgtccgaa catatttcca gtattttcag acggcatttc aagttttcaa
    23521 atccctcaat gtccaccgcc gccgctgtgg aacggcggtt tcgccagcca gacaatggga
    23581 atcaggacga taaacaaaat gctgccggcg aggaatattt cgttcgatcc gatgatgaag
    23641 ccctgttgcg taatggtatt gttgatgatg cctagagttt gaccgtcgga aatgccttgt
    23701 tgggacaaat gcgccgccgt ttcgtgcaat gttgcggaat agggcgtgat gtgttcggca
    23761 aaacgcgtgt ggtgcaacgc ttcgcgccgt tcccacaggg tgctgacgac ggatacgccg
    23821 acaccgccca tcagcacgcg caggaaattc gacaggctgc cggcggcggc aatctgtccg
    23881 cccttcatgt gcgacagtgt gatggtggtc agcggcagga agaacatggc aacgcctacc
    23941 ccctgccaaa actgcggcca gatgacgttg ccgatatcca tgtcggcata aaaatcagtg
    24001 cgccaataaa aagtgaaggc aaaggtcagg aagctggcgg ttacgagcag gcgcatatcg
    24061 attttattgc cgaacctgcc gattaacgga gataggaaga cggggaggat gccgacgggc
    24121 gcggcagcaa gccctgccca cgcggaggta tagcccaagt tggtctgcaa cactaacggc
    24181 agcagggtca gcgtccccat atacaccatg aaacccaacg aggtggcaat tacgccgacg
    24241 gtaaaattcc gatctttaaa cagcgataaa tcgacaatcg gatatttttc tcccaattcc
    24301 caaacaataa aatacgacaa gcacaccagt gcgactacgc ccaaggtaat gatttctcca
    24361 gaggcgaacc agtcaagttc cttacccctg tccagcatca tctgtaacgc gccgataccg
    24421 accatcatca gcgtcagccc gacatagtcg gtcggcgttc tgacggttgc cgtttcgcgg
    24481 tgtttcaaat gtttccatgt aatccatgcc gatatgatcc cgatggggat attaatgaag
    24541 aaaatccaac cccaatgcca gtttccggaa atccaaccgc cgagtatcgg cccgaggaca
    24601 ggggcgacaa cgacggtcat tgcccacaat gccagtgcca gcatccgttt tgcgggcgga
    24661 taggatgcca ttaacaggct ttgcgacagg ggaatcaacg gcccggcgat aaagccctgc
    24721 aaaatgcgga aaacaaccag cgactgaagg ttgggcgcga tgccgcacag ccacgatgcg
    24781 atgacgaaac cggcggcggc ggcggtaaac aatttgacct cgccgatacg ttttgccaaa
    24841 aagcccgtca gcggcacgga aacggcgttt gccacggaaa aagaagtgat gacccacgtc
    24901 ccctgcgtgg ttgccgcacc gaggttgccg gcaatgacgg ggacggcgac gttggcgatg
    24961 gtcgtatcta aaacctccat aaatacggca agccccaaag acagcgtaac ccacgccaat
    25021 gccgcaccct taagcggtgg ataatccatt gtgtcctccg tttcagacgg catcagggcg
    25081 cggattgccc gaggatttca tcgaccgtcc gatcgacttc cgaccagtcg gtactttcca
    25141 tttccggcaa tgccgcaccc ggcgtttttg aaacaggcgc gcctgcggcg gaagtatcca
    25201 ctttaaccgt catcgacaaa ccgatacgca acggatgcct gtccacatct tcgcggttca
    25261 gcacgatacg gacggggacg cgctgcacca ctttaatcca gttgcccgtt gcgttttgcg
    25321 ccggaatcag cgaaaacgcg ctgcccgtac ctgccgaaaa ccctgccacc ctgccgcgat
    25381 aaacaatttg tttgccgtac aaatcggaca ccagctcggc aggctgtccg attttcatat
    25441 gccgcaactg cgtctcttta aaattggcat ccacccacac atccgacagc ggcaccaccg
    25501 ccatcagcgg cgcgcctgcc gccacctgct gcccgacctg caccgaacgc ttcgccacct
    25561 gaccgtccgc cggcgcgcgg acttgcgtcc gccgaaggtt cagccacgca tctttcaacc
    25621 tgccgattgc cgtctgaacc tccggctgtt cgcgcaaaga aacatctccg cccaaagccg
    25681 cgcgtgccga agattcttcc gccaaagccg ccttaaccgc cgcctgcgcc tgagacactg
    25741 ccgtacgggc gtgtgccagc tcttcggcgg acaccgcacc cgattccgcc aaagcggacc
    25801 ggcggcgcaa atcatcctgc gcgcgcgcca aatccgcacg gcgcaaggca acctgcgccc
    25861 ccgcctgcga agtggcggca ttttgccggc ggttttggcg caccgcctga accaactcgt
    25921 tttttgcccg ctcgtaagcc agcacatcat tatcgtcatc caacaccgcc agcacgtcgc
    25981 cttttttcac ggcatccgta tcgtcgtgca aaaccttccg caccgtgccg cccttttgcg
    26041 gcgtaacctg aaccacgcgt ccggcaacat aagcgtcttc cgtttcctct tcgtgctgcc
    26101 accataaaaa aaacgccgat ccggcggctg cggcggaaag cgcgaacagc agcgtcaatg
    26161 ccgtcaggcg gcgtttgcgt ttgacttgcg tgttttgaag ttttgtttcg tctgtgtgcg
    26221 tttccataag attgggttcc gaatttcctg atttcatata aacaatgttc tttcaatagg
    26281 aaatagattt tatatccgcg caataattca atcaagtcag tattttagat gcaatattca
    26341 aaaaacaacg cttcaggcgg caaaactgaa aatatatttc gtttatatat gacatggcaa
    26401 acaccgccgc ccgcaccatg ccgtctgaag aaaactacac aaataccgcc gcttatatta
    26461 caatcgccgc cccgtggttc gaaaacctcc cacactaaaa aactaaggaa accctatgtc
    26521 ccgcaacaac gaagagctgc aaggcatctc tcttttgggt aatcaaaaaa cccaatatcc
    26581 gaccggctac gcgcctgaaa ttctcgaagc attcgacaac aaacatcccg acaacgacta
    26641 tttcgtcaaa ttcgtctgcc ccgaattcac cagcctctgc ccgatgaccg ggcagcccga
    26701 cttcgccacc atcgtcatcc gctatatccc gcacatcaaa atggtggaaa gcaaatccct
    26761 gaaactctac ctcttcagct tccgcaacca cggcgatttt catgaagact gcgtcaacat
    26821 catcatgaaa gacctcatcg ccctgatgga tccgaaatac atcgaagtgt tcggcgagtt
    26881 cacaccgcgc ggcggcatcg ccgttcatcc gtttgccaac tacggcaaag caggcacaga
    26941 gtttgaagca ttggcacgca aacgcctgtt cgagcacgac gcacaataaa ccatccttcc
    27001 catatttcag acggcattcc ttcgatacgg tatgccgtct gaacgccttt ccgaaaggtt
    27061 tttatgtacg cattgaccgc cgcacagcaa cagaaggcac tcttccggct ggtgcttttc
    27121 catatcctca tcatcgccgc cagcaactat ctggtgcagt tccccttccg gattttcggc
    27181 atccacacca cttggggcgc gttttccttt cccttcatct tcctcgccac cgacctgacc
    27241 gtccgcattt tcggttcgca cttggcgcgg cggattatct tttgggtgat gttccccgcc
    27301 cttttgcttt cctacgtctt ttccgttttg ttccacaacg gcagttggac gggcttgggc
    27361 gcgctgtccc aattcaacac ctttgtcgga cgcatcgcgc tggcaagttt tgccgcctac
    27421 gcgctcggac aaatccttga tattttcgta ttcgacaaat tacgccgtct gaaagcgtgg
    27481 tggattgccc cggccgcatc aaccgtcatc ggcaatgcac tggacacgtt agtatttttt
    27541 gccgttgcct tttacgcaag cagcgatgaa tttatggcgg caaactggca gggcatcgct
    27601 tttgtcgatt acctgttcaa acttaccgtc tgcaccctct tcttcctgcc cgcctacggc
    27661 gtgatactga atctgctgac gaaaaaactg acggccctgc aaaccaaaca ggcgcaagac
    27721 cgccccgtgc cctcgctgca aaatccgtaa aacagctctg tagggaatcg ggacggttgg
    27781 gttttctttt atttgcaaac actgccggaa cggataaatc cggattccca cccacttcgt
    27841 cattcccgcg aaagcgggaa tccggtgcgt tggggttcag tcatttccga taaactcctg
    27901 ccgcattgta tttctagatt cccgctttcg cgggaatgac ggcggatggg tttccgtgcc
    27961 gtggtgtccg gattcccgcc ggtacgggaa tgacggcagc gcttccttgg cttagtttta
    28021 ttttaagcca ctataaatgc cgtctgaaaa cctgtttgca gattttcaga cggcattgtt
    28081 ttcgtcgtcg gttcaaactt caatccgatt gcgacacggt aaccggtatg ccgcgcaagc
    28141 gcgcaagcag cgcgtccgcc ttctgtttct gcgcggcaaa cgccgccttg tccgcctgcg
    28201 tcaattcggg tgtcggcaat gcgaccgaaa caggattgac gggctgcccg ttgatgcgcg
    28261 cctcgtaatg caggtgcggc ccggtcgaac gccctgtcga accgacaaaa ccgatgacct
    28321 cgccgccgcg cacattgcct tgtgcctgcg aaaacgcgct caagtgcgcg tacagcgttt
    28381 ccacaccgtt ggagtggcgt atcatcaccg cgttgccgta tccgcccttc cggcctttaa
    28441 aggtaatcac gccgtcggcg gaagccctga ccggcgttcc ctgcggtgcg gcataatcga
    28501 tgcccgtgtg cagccgccat gtgtgcagga tggggtgcat acggtagccg aacggcgaag
    28561 aaatgcgcgt atagaccagc ggctcgatgt tgaagccgcc tttttcctgc aacaccctgc
    28621 cgtcttcatc gtaataattg ccgccccctc cgccttcctt gtccgaacgg taatagaacg
    28681 cctgatgggt tgtgtcgccc ttgacaactt ccgccgccaa aatatcgccc gccgccacct
    28741 gctgcccgtg gaaatacagg ctgtcgtaaa gcaggcgcac ggcatcgcct tccttcaaac
    28801 cgtcaaggct gaagcggccg gcaaaaatcc cgcttaagga ttcgcggatt tcgacgggca
    28861 cttccgcccg cgccagcgaa ccgcgcgccg acgttttgac cacgaccgaa cgcagtgtcg
    28921 gcaaaacctt catatccgca tcagaagccg accgccgcca tatgccgcct tttttttcca
    28981 aagcgaccag attgcgctcg ccgtcttcgt cggtaaaaaa ctgcacttcg cgcgcactgc
    29041 cgtcgccgcc gaccaaaaca tgaaccgact ggtcggcacg caaatgccgc aaatcggctt
    29101 cgccgccata tttttccgtg attcgggcaa tctcgtcccg cgccataccc gaacgcgcca
    29161 gcacgtccgc cagcgagtcg cccggctgca ccgcctcctg cacccaatat gccgtctgaa
    29221 cgccgctgcc gccccaagac agcggcggca gtttttgttc cacgcgctgc ggtctgacgc
    29281 gctccgtccc ctctgtcgaa gcaatgtatg ccgccgacac cagaataatc gaaacggcaa
    29341 gcgcgcgcag cgcgtatttc cgatgttttg ccgaaagtgg gaagacagcc atattgttcc
    29401 tttttgacgg cgtttgcgcc gaatcctcta taatatccgc ccgatcctca tttgtcaggc
    29461 atcaaacgat gaaactcacc ctcgccctcc cctcgctcaa tttggatgaa gacgaaatcc
    29521 gaatacctct atgcctgccc gccttcaaca aaatcctgca atacggctcg ccgcaccgcc
    29581 aaagctgcac ggcttcggca ttttacgcac gttatttgtg gtgcggaaga ctggcacaac
    29641 gcccggcgca aagcctgaat atgccgtctg aaaccgtcgc gctcgccacg cccgtctggc
    29701 aaaaaatggg gctgcatcag gcaaatgtgc tgacggcgga atatttggat gtcggtacgg
    29761 atgaagcgga acggctctgc cgcgatctgt cggcgtttta cggcgacatt ccgtggcgtt
    29821 tcgttcccgt cctgcccgaa ttatggctgg tttcccttcc ccgcgcgtac cgctggggcg
    29881 cgaagcccgt tttggacttg ggcggactgt tgggcgcgga cgatcagccg gacggggaag
    29941 acgcgttgga atggctgcgc gtccaaaccg aaatccaaat gtggctgaac gctcaccccg
    30001 tcaaccacaa ccgcaaaaaa cgcgggctgc ccgaattgaa cggcttatgg ctttgggaca
    30061 gtctgcacgg aagcgcacaa ggcggcacgc ttttcgccga tacggtttgg agccgttttc
    30121 acccgaaccg ccgagcattg cccgacagct tccgagctta tgcggaaacg gcggcacacc
    30181 tgcccgatac gcaccacatc ctgtttatgg acgatttgcg cctgaccgcc ctgacagggg
    30241 acagggagcg gtatgcggca atattgcagc agtgggaaga acgctggttc gcaccgcttt
    30301 acgaagccgt ccgcaccggc aaaatcaaac ggctggacat cgccacggac ggacaacacg
    30361 gcggtacgct gaccttcaaa cccacagaca ggcggaagtt ttggcgctgc acgaaaacct
    30421 ttgacggaat ctggtaaacc cgcccgccgc aaccgcgaca atgccgtctg aagcccgaat
    30481 cgggtttcag acggcatttt accccgcatc gccgttatgc cggaaaaacc ggaatacgtt
    30541 tccgcacacg cggcggcacg caaaacaccg cccgaaagcc gccgatgccc gccgtgacac
    30601 cgccgccggc agggtttccg ccgaaaagga acagcaatca gaacgttttg ttaaactcga
    30661 caaacgccct gtttttctcg tattcgttaa acacatcgtt gctccacgtt tcgcggtgcg
    30721 acagcgtcag gcgcggcgtg atgcctttga aatgcaatgc ccggtgccaa aggctcaagg
    30781 atgtgtccga ttctttatcc ctgcgccttt cccctttaaa actgctgaag aagccgggtt
    30841 tttcataatg ccgtttcgcc acgccgaggc ggaacagcga agacaggccg ctgccgcccc
    30901 attcctgccc ccaggcaaag cgcaggccgt aacggttgaa attgtcgcca cggtcggcgg
    30961 ggttgcgctc gcggtaaaaa tccaaaccgc ccgtccaata ttggcgcgca ttccggtaaa
    31021 acaccagcga attggaaatt tgcaaatggg tattgtcgga acgcgcccgg cgcgtattct
    31081 tcaaacgccc ccactccgcc gaagacagcg tttgccatct cggggtttgc caacggttga
    31141 aataaaggcg tgcgccgttg gcgtaagaat aagcgtcgtt gccgtaggtg cggcgttcgt
    31201 ggaacactgc cagcccgaca tctttacgcc ggtcggcaaa accgatgccg ccggaaacac
    31261 ctgccgtcat atcgttgaat ttcttattcc ccggataaac cctgccggac acgtcgccgc
    31321 ccgccgtcgt gtaccagccg tttttcagcg accatttttt ctccgcgccg aaccggtaat
    31381 tgaccgccgt gccgtccacc tgtttcggga aagtccaatt gccgtactgc tgctgtttcg
    31441 gggcttggtt gatattgtgt tcgcgggtaa cgctgaaacc gccgtttacc ttccacgcat
    31501 cgcgttcgcg caatgccttg cggtacagct cgacctgctc cataagctgc ggcggcagat
    31561 cttctgtttt caggcggtcg aactggtctg ccgccgcctc gttctgcctg tcttcaaaca
    31621 atgccgccgc caaacgcata cggacggcgg gcgcgtcggg ttgggcggca atcaattccc
    31681 ggtaatggga aaccgcctcc ttcaccctgc cctctgcctg cgccaaaatc ccttgtgcat
    31741 aaagtgccaa catcttatcc tgccgcgcct gttgtaggta aatcggcaaa atcacgcgga
    31801 taccggcaat attgtttgag accactgcgg aatacatggc acgcgacaac aattcgggat
    31861 ttttcagcag ggtttcgccg tcaacctgca aaacttttcc tttttcccgc acctgccccg
    31921 gtaccttctc cctgtcgatc ggtttgacct ccgcttcatg aagcctgaac tcgggacggc
    31981 ttctcaaatc cggttcgcgc ggtgtttctt cggcgtatgc cgcgcttgcc aataaaggca
    32041 gcagcatcca tctgtttcgt gcaggcataa atgtcttccc acaaaaataa aaataaaaaa
    32101 taaccattat caaccggcgg attgtatcag ttttttatgc cgctgttcca aaccctgccg
    32161 cccgatgccg cgacaatgcc gtctgaagcc agaatcgggt ttcagacggc ggcaaaaaac
    32221 cggcggtttt ccgccggttt catcattatg ccccgccctt ctgggcggca gggttcggat
    32281 cggtttgcgg ggcggcgcac ggggccgtgc aggtgtgccg cctgtgtgtc aaaacatttc
    32341 ccggcgcatc aaaacaacca tttgaccgac aatgcggctt ctttgtcgcc gtccgtcctt
    32401 ttgccgtatc cgatgcgtgc ggacatatgg cctttccaac cggcttcaat gccaaaccgc
    32461 ccttcgagcg ccgtcctgcc tgccagcgtc tgtttttcgc cgtccatttc catgccgaaa
    32521 gattttgacc tgtgcaaaac attaaaagcg gcaaaaggct gaagattgac accgttacgc
    32581 aaagcaaaac gggtttttgc ccgaatgccg gcgcggattt gccactgacc gctgccgagc
    32641 agcccgaccg ccgcccctcg ctgtcggtaa agccgccgtt tacgcccaag taggtaaact
    32701 gcgcctgcgg ttgcaggtaa aactgcatat tactgcctta tatattgcgt ccctaagaag
    32761 gggcgattaa caaaaattaa cgccccttct tagggacgca atatataagg agtgagtacc
    32821 tgttcttcta ttaaagaatt aatcttttga ggcattttct gttgaagttt attaaacaca
    32881 tcttcagaaa ttccaaactg ctttattatc tcttccctaa cccgtttttt attttcttcc
    32941 caagcctcgg gtcttgtttt gtataaatcc tgtaattgct ttttaactgc ctgctttaaa
    33001 tcagttcggt aattatctgt aatattgatt tcgtcataca aggcttcaga tgatttttga
    33061 tcggttttac cgctggagaa ataatggaaa acaccgtttg ccggcttgtc tgaaaatgaa
    33121 aaatcggcag tatcgccaac atttgtagga cgagtatccg aaccgggctg ataactgaaa
    33181 gcaccgaagc aatcataaag atatgctgca cgggccgcgc caaacgtggg aatttctgtc
    33241 ttccgccgct tctaaaataa atttgttttt aatctcggct tctttgacgg gatcgtacgc
    33301 atccgaaccc caagcatatg gcgtgcaaaa cagcgataaa acagataatg ttaatagatt
    33361 aacccgaccg cttgctttca gtgtcatgag aaattttcct ttgtcaagtg ttaaagttat
    33421 aatgattata tactatatca cactacatcg caatgaaaaa tcgggaaaaa caaaaaaccc
    33481 ctccgccgtc attcccgcga aagcgggaat ccagaatttc aacgcctcaa gaatttatcg
    33541 gggatggccg gggtttaaag gtctgggttt ccgcctgcgc gggaatgacg gtttagaaat
    33601 cacccgaaac gagaaaaaga accgaaaccg gacaagtcgg attcccgcgc gggcgggaat
    33661 gacgataagc gtaatttcaa tccgccatat tgtcttttca cttgcctgaa aacagatttc
    33721 agccgcctat ccctgcatcc ggcggctgat gtagtcggca atcaaatccc gcttttccca
    33781 aaaaaacttc tgcatttttt gttcggaata cagtttgcgg accagagtgg gcagaccgct
    33841 gtggacgatg gcggcgattt ctttgttgcc ttcttcttcc tgcattgccg tctgaaaatc
    33901 caaacccctg cctttcagat atgctgcaag gcggttgcgg atgtgtgaat tgagttcgat
    33961 ggatttgagc attttgcctc cgttttgcgg taaggattta ttcgatttgt ttatccgcct
    34021 gattttatca cgtttcccaa gcggtttgcc tgtacaatac cgcttttcgg aagggtgtgg
    34081 gcgatgatgt tttcttggtt caagctgttt cacttgtttt ttgtcatttc gtggtttgca
    34141 gggctgtttt acctgccgag gattttcgtc aatatggcga tgattgatgc gccgcgcggc
    34201 aatcccgagt atgtgcgcct gtcggggatg gcggtgcggt tgtaccgttt tatgtcgcct
    34261 ttgggtttcg gcgcggtcgt gttcggcgcg gcgataccgt ttgccgccgg ccggtggggc
    34321 agcggctggg ttcacgtcaa actgtgtttg ggcttgatgc tcttggctta tcagttgtat
    34381 tgcggcgtgc tgctgcgccg ttttcaggat tacagcaatg ctttttcaca ccgctggtac
    34441 cgcgtgttca acgaaatccc cgtgctgctg atggttgccg cgctgtatct ggtcgtgttc
    34501 aaaccgtttt gacggagcat cttatgccga tagaaatcga acgccgtttt ttgattgaaa
    34561 acgacaaatg gcggcaatac gccgatgagc cgctgctgct gaaacagggc tacctgtctg
    34621 ttgaaaaaga acgcaccatc cgcatccgca ttgccggaaa acgggcgtgg ctgacgctga
    34681 aaggctatat ttcggaaatc agccgcagcg agtgcgaata cgaaatcccg cttgccgatg
    34741 cggaaaagat gatggaaacg atgtgcccgt ttaagatgga aaaacggcgt tatccggtca
    34801 gatggggcgg cagcctgttt gaagtcgatg ttttccttgg cgaaaactcg cctttggtcg
    34861 tcgcggaaat cgagttgccc gccgaaaatg ccgattttga ccgtccggac tggctggggc
    34921 gcgaaatcac ttcagacggt atgtttacca atgcctattt gagcaaacat ccgttctcaa
    34981 gctggaaaaa tgccgtctga agcggcttca gacggcatct gccttgcggt tatcccttga
    35041 ggtaatgcac gaatgtatat gcaacgcctt tgctgctgac acggcgttcc gtccgctctg
    35101 cttctttcca atgcgtccgg tctatttctg ggaaaaatgc atctccttcc acagacaaat
    35161 ccacttcggt tatccgcaaa tcggtcgcta atggcatcgc ttgtccgtat atctgcgcgc
    35221 cgcccataat gacggcttct tccgcgccgg cgcacaatgc caatgccgcc tccaaacttg
    35281 ccaccgtttc cgcgcctgcc gcgcaataat ccgcctgccg gctgatgacg atgttcctcc
    35341 gtccgggcag gggtttgacg ggcagggatt cccacgtttt ccgccccata atgacgggtt
    35401 tgcccaaggt ataggctttg aaaaatgcga aatcttcggg gatgtgccaa ggcatagcat
    35461 tgcccgcccc gatgcacagg ttttccgcac acgccgcaat tatggttatt ttgagcatat
    35521 tttctccaca aaaatgccgt ctgaacgctt cagacggcat tttatgacat tctgcgtcaa
    35581 aaatctcaac ctgcgcgcgc catacgtttt gtgttcgcac ccgccaacaa tgccaacaat
    35641 tcttcagtcg cgccccaacc gatacacgca tcggtaatgc tcttgccgta cacttccggc
    35701 ttgtcctgcc tgccttcgac caaatggctt tccaccatca cgcccatgat attgccgccg
    35761 tcctgttcca attggtcggc aatgtcttgt gccacttcca tctgccgggt gtaatccttg
    35821 cggctgttgg cgtggctgca atcgatcatc agcttgtcgg ttacccctgc cgcacgcagt
    35881 tgttccaccg cctcgctgac gtgtcccgca tcataattcg gctcttttcc gccgcgcaaa
    35941 atgacatgac agtcgggatt gccgccggta tggacaatgg cggaatgccc ggccttggtt
    36001 acagacaaga aatgatgcga atggctcgcc gcaccgattg cgtcgatcgc aatcttcaaa
    36061 ttgccgtccg taccgttttt aaagccgacg gggcaggaca gaccgcttgc caattcgcgg
    36121 tggacttggc tttcggtcgt ccgcgcgccg attgcccccc aagaaatcag gtcggcataa
    36181 tactggggtg taatcatatc caaaaattcg gtagaggcag gcatgcccat attattcagg
    36241 gacaacaaca ggctgcgcgc ctgacgcaaa ccgaaattga tgtcgaacgt accgtccaaa
    36301 tgcgggtcgt taatcaaacc tttccaaccc accgtcgttc tcggcttctc gaaataaacg
    36361 cgcatcacga tcaaaagctc gttttcatac cgcttgcgga gtttcaacag acgttccgca
    36421 tattccaacg ccgctttcgg atcgtgaatc gaacacggcc cgataatgac caacagccgc
    36481 ctgtccctgc cgtgaaccaa atcggaaatt tcctgacggg tgcggtgaac caagcccgaa
    36541 gcctctttgg aaatcggcag ctcgtaaaga tgggcaatcg gcggcaacaa ctctttaact
    36601 tctttaatct taatatcgtc tgtggggtaa tggtgtgtca tcttgcatct ttcctgatgg
    36661 gtttttatac gcggcttagg ttacatcgtt taccttgcac aaacaagtgc aacatattac
    36721 tacaaagcta ttttatttca aaataactga tatatctttt ttcatagatt ttaaatttca
    36781 tatttttaat attttaggaa ttttatttca aagaaaaaga aacagattga taaaaacaat
    36841 acccgccatt cattcgggca aaaccgcacg gaacatccgt tttcccgttt ccacgacaat
    36901 ccggtacagg acaccccgct gccgattccg cctgcacatc cggcaaaatt tcatacgcaa
    36961 ggaaaatgtt gaaaacaaaa agaactaatt gcttgaaaaa agatttaatt ggaatgcggg
    37021 tatttcccac actttctcca tttatttatc ctcattataa aaacatgaaa aaaaacccct
    37081 gcttgcactc tcacttgcag ccgtgtccgc cgttacggta gccgatattc ccgagtctaa
    37141 cggcaaaggc gaatacaaag tctttaccgc ttatgttaaa ggcaaagcca ctgccggcaa
    37201 tttgaaagaa aaataacgca caccgtccat cggttttcgt tcaacgaacg ccacgatatg
    37261 cgttttaagg cagagtacgc ccgaagcaaa aacgatgcag acgacaaaag agggaatgca
    37321 cacatcgcta acggcgcaat aggcaaaatg gctgccgtgt atgccggtta tacctacacc
    37381 caaccgggcg gcgaatccac acgtttccgt ggcggtttgg agcgtgtcca agttaaacgt
    37441 tatctgggag aaaaatgctg cggctacgcc tgtccaaaaa gcgaacgctt caccctgcaa
    37501 gcagatggtg aaatccgtca aaaccttggc gaaggctggt ttctgaagct atgagcgggg
    37561 tggctgtcta tctccgcaac aaaaacaaaa tagcaattgc ctcccaagat gccaacctaa
    37621 actccaaagg ccggttcgtc agcagcggtc tgaatgtggg caaacaactg accggcagtc
    37681 tcggcgtaga gtttgaccca tactaccgcc accgcgcaat ccgcaagtct gccgaatttg
    37741 tcccgaacac taccaaaacc aaaacagaca gcgaaaagtt taacgagtag ggcttccgcg
    37801 tagccgcaac gttctaattc ccttacgtat aaaaagcagc ataaaaatgc tgccttttcc
    37861 catttccaag ggaagtattc cgtagtgcag cttacctcat tgtttcacac aaaccgaaaa
    37921 tgccgtctta aaaccaattt tcagacggca tttgtccctt aaaacacgtt ttttcaagcg
    37981 cgaccatacc aaacacccac aggttgcctt ccacccttcg tacaaccttt gctccatcaa
    38041 acatccactt cctaaatact gatgtatact g
//
LOCUS       DAWTCI010000010        36289 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWTCI010000010 DAWTCI010000000
VERSION     DAWTCI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 36289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36289
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.010"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            416..1753
                     /gene="ribB"
                     /locus_tag="WM481_000551"
     CDS             416..1753
                     /gene="ribB"
                     /locus_tag="WM481_000551"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFA9079935.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQNRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTGRPPGHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAVNFMIKHARGLVCLPMEGAMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGDAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHHTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLHVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(1942..3543)
                     /locus_tag="WM481_000552"
     CDS             complement(1942..3543)
                     /locus_tag="WM481_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFA9079936.1"
                     /translation="MTEQHFTEQIKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKEKPDEPIEFVNMDIDGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAACNADLFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHVIGEDKCMIYTQDISQKSSNLLRLNLSLNNLVHSLNNVVQG
                     NTILSPAHKDASGCLKKFDFIVSNPPFKLDFSDFRDRLESDENHERFFAGIPKIKPTK
                     KEKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAKSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKVNKDKVVLIDASGLGEKISINDNQKTVLSHEEEQKICH
                     TFTHKQAVEDFSVVVGYDEIKAKNHSLSAGQYFEVKIDYVDISADEFVQKIAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(3610..4449)
                     /locus_tag="WM481_000553"
     CDS             complement(3610..4449)
                     /locus_tag="WM481_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079937.1"
                     /translation="MNFVLAKITCKKDLVKILSDDHIFPDFSYENLNFITYNYDYNLD
                     DDTWFQIENLKNQDFCPKFLDNSNLFDSKMFSEIKKEEINIEKLKYLVSCTNDALFFQ
                     KITSSLLLKKKHLLTICGNGAKLCEPQDLLVIKDIPDAVYIIKDDKLIFRTLSSISNI
                     FKGIEDLYREATNTEVQQFLESDFIDLKEDFLSEKVSIPNRKRIALVQDRLNNMTLDQ
                     RQELLNYLAEYNNILKFNADGSRVEISTDVQLKHLLYGIDERYYTTALGKEKRLANSV
                     QPI"
     gene            complement(4460..5020)
                     /locus_tag="WM481_000554"
     CDS             complement(4460..5020)
                     /locus_tag="WM481_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079938.1"
                     /translation="MDKLFKILLNISSTSLLLSIYFIKSQYYILDLTWFYRVISNHWI
                     EPISKLSLLFYFLIPFIATATVLWLSKYLGKDEFKQGEVKELEYVNDNFLPSYLGYFF
                     VALSIPDNNLFLLFVMYGIIFLLVSCSKSFYFNPVFFLFGYRFYQAKTESGLLLVLIS
                     KQEFRTPQSVSSIAVYRINNFTFLEK"
     gene            complement(5013..6215)
                     /locus_tag="WM481_000555"
     CDS             complement(5013..6215)
                     /locus_tag="WM481_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9079939.1"
                     /translation="MNHFKKQQIQNIADFNPREQLAKGALAKSVPMAMLKEFQRQITG
                     YEIKAFNGGAKFRNGDTLLAKITPCLENGKTAFVDILDDGEVAFGSTEFIVLRAKNET
                     NPEFLYYFAISPDFRKRAIECMEGTSGRQRVNENALKTLELPIPEPQIQQSIAAVLSA
                     LDKKIALNKQINTRLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGDMVFDETLKREIP
                     KGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNPQDA
                     HIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHF
                     KFLKEFKIILPSKDISQKYNEIANTFFVKVRNNLKQNHHLTQLRDFLLPMLMNGQVSV
                     RCSGARDG"
     gene            complement(6265..6495)
                     /locus_tag="WM481_000556"
                     /pseudo
     CDS             complement(6265..6495)
                     /locus_tag="WM481_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(6544..9549)
                     /locus_tag="WM481_000557"
     CDS             complement(6544..9549)
                     /locus_tag="WM481_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9079940.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNANWDRQTNIFPEI
                     FVDSLCRINPDLPPDDARRLLADIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEAFRPDIALLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     PKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSACGKPVFNYFREEHKLNLTEL
                     LDTLSDDLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLAFLLQHGL
                     TYVKASQGSVQKHIMRYPQLFATLAIEKHLSNGGKKGVIWHTQGSGKTAPAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLIVHTIDSREAFAADIKSAQTLHN
                     HAGKPEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKEERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTAKELYNILRLQGSHEFLAHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDLEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQTVYTKIKEPNRQNDLLRHKYGGDAKYARIH
                     KRLMENAALYGDKLKVFNALNGVKTDADQRVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQTINRLLVREYLKESGRI"
     gene            complement(9669..10979)
                     /locus_tag="WM481_000558"
     CDS             complement(9669..10979)
                     /locus_tag="WM481_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFA9079941.1"
                     /translation="MTDLFVREPDAPLAERLRPHTLDDVIGQQHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYRDFTIEADVQELLVNTADGDARRLLNLLEQLLRAADTR
                     RLKILTTEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            11250..12227
                     /locus_tag="WM481_000559"
     CDS             11250..12227
                     /locus_tag="WM481_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079942.1"
                     /translation="MELITTGEGADGDLLVNGSYGIRSFADLKLEYKPELSVRSNHDI
                     IFNNDFQSVDVQPYSGNIHLDAKGKIQFNLNVADKVTDPTTHQEKLVNDNALVRYVNN
                     ITPSDENDKGSISFHSKTGNLFNMNVNVHKGTHHFGIHNLRNTNVNLTAEDGDNVVAI
                     KGKLAEPMPAGKVESTSFLRGIETAFKSNTVLTGNNNKISITTEGDGLGLGVNGLQSN
                     SPYSGHTTGTFINNQPSNTTQPSLDGTSTIELKAVKGNNEVDLNVKNHASVKGIITSH
                     SAKATLEAEEDNIVRVKNPDTKTALINAWKEKYPNNSPEPYRVGGSIHD"
     gene            12157..12810
                     /locus_tag="WM481_000560"
     CDS             12157..12810
                     /locus_tag="WM481_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079943.1"
                     /translation="MPGKKNTPIIRQNHTGWGVQSTIDSDVVLSAANNIVEVGGSPMN
                     QEGITAHGNATITLKAKENNKITVENAAYSSDGISTLINRTGARPGTRDDGNKIILEA
                     GGDNIVTMKSGDADADYVNNSKVLTETPYYKSKRGSNGIFAYGDKSLVKLIGENNIVK
                     SEISEKSKALNGGFRHIGIYSWQNAKVELSAKSDNIVQGGIWGLYSNNSSISLKGKK"
     gene            12928..14532
                     /locus_tag="WM481_000561"
     CDS             12928..14532
                     /locus_tag="WM481_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9079944.1"
                     /translation="MNYKDDTRITGATVSDKGLVAIKPLNNTNIVADTIHYKGDVLAV
                     NKGKVELDFTPNIRLVGRLDNFSGLTDSKHKNLFENYVANLDSKSAGEINFNLAKDAL
                     WTMTGQSWLDKLEGQGTIDFNNDAKTSGRALHIGELAGANKFLMHLNKDGIHSDMLYV
                     KKGTSTPQEVVVKNLSEVLDSMNYGERLRFATVTNSKNEFVNGKKYIDDTHLMEDALT
                     VEYSAHNGDKNNKDDYNKSFNGSEMTAEKAGDDYVNKTYTDNRQNVYLVKQATGNPSR
                     NVKNINDMFDSTAHYAFTLDTYAKREGERAFSTLDKKEGDWIRLTHTRVIQSNAFRFH
                     NNDFEIGYDRFSLNEQEKKRKWGISLDYGHGRTSLWNTFGKDKIRKYELALYNTTQYI
                     DKEGDETGYIDNVLKIGKLRNRVIARNHMGQLWGKGKYSNTLFSISTEYGRRKFLDDD
                     KLWRITPQVQLQYSYLRGTGYRIDNGINVNLSHANSLIGRLGLDVVRKFDGGKKLFYI
                     KGNIFHEFLGSRSFKAFEGKSHYAQK"
     gene            14563..15034
                     /locus_tag="WM481_000562"
                     /pseudo
     CDS             14563..15034
                     /locus_tag="WM481_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15060..15976)
                     /locus_tag="WM481_000563"
                     /pseudo
     CDS             complement(15060..15976)
                     /locus_tag="WM481_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            16231..17694
                     /locus_tag="WM481_000564"
                     /pseudo
     CDS             16231..17694
                     /locus_tag="WM481_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
     gene            17945..18313
                     /locus_tag="WM481_000565"
     CDS             17945..18313
                     /locus_tag="WM481_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079945.1"
                     /translation="MNKTLSILPAAILLGGCAAGGNTFGSLDGGTGMGGSIVKMTVES
                     QCRAELDRRSEWRLTALAMSAEKQAEWENKICGCATEEAPNQLTGNDVMQMLNQSTRN
                     QALAALTVKTVSACFKRLYR"
     gene            18386..19795
                     /locus_tag="WM481_000566"
     CDS             18386..19795
                     /locus_tag="WM481_000566"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFA9079946.1"
                     /translation="MRYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMCLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     CLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIRQVYEQDKELIDPH
                     TANGVKVAREVREAGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            19842..20636
                     /locus_tag="WM481_000567"
     CDS             19842..20636
                     /locus_tag="WM481_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079947.1"
                     /translation="MTLPMQETRFSILLDELAAKQEATIAPYLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKRQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAGTVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            20824..21600
                     /locus_tag="WM481_000568"
     CDS             20824..21600
                     /locus_tag="WM481_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9079948.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCIRDESLRFENGQFVMVGLMA
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDEVLISKKPTGTLVAC
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHRGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(22259..24730)
                     /locus_tag="WM481_000569"
     CDS             complement(22259..24730)
                     /locus_tag="WM481_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFA9079949.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCVGCQAVAQSIIDS
                     GLGSYYKRRTADAKKTELPPQEILDQIRLYDLPEVQSDFVETHNGTHEAVLMLSGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQTGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRAGMDTPIAAAIIMTFIAGIYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVRESAVVKLQ
                     AGDIVMVKPGETIPVDGTVLEGNSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLSQTTD
                     IIFDKTGTLTQGNPAVRRIELLGSMTEAQVLAVAQSLEQQSEHPLARAILNCRISGGS
                     VPEIQVGQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLQDPLKDSAAEAVRQLAGKNLTLHILSGDREEAVAETARALGIAHYRA
                     QAMPEDKLEYVEALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKILSEQ"
     gene            complement(24727..25905)
                     /gene="hflX"
                     /locus_tag="WM481_000570"
     CDS             complement(24727..25905)
                     /gene="hflX"
                     /locus_tag="WM481_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFA9079950.1"
                     /translation="MSGRTGRNSATQAQPERVMLVGVMLDKDDTGSNAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPACSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAHDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAEYCAAAPNTDETEMP
                     "
     gene            complement(25911..27191)
                     /locus_tag="WM481_000571"
     CDS             complement(25911..27191)
                     /locus_tag="WM481_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFA9079951.1"
                     /translation="MKSLFIWLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRTQIGLHALAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDALMSAVYHRLSLLDRHTDE
                     AGAAFVRENGKTVLIFNQGNGSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMP
                     VTELLYTAYPVGGGALPYFYGERPDPVPEYEITGNPASIDFSEAAGKIAMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAHQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRRQFGNSLSILRHE
                     AGGIVFSVSGMAGSRIRLTPEDSPERGVTLYLQD"
     gene            complement(27343..28068)
                     /locus_tag="WM481_000572"
     CDS             complement(27343..28068)
                     /locus_tag="WM481_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFA9079952.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENMRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVAYWDFNWRKGGGGARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPV
                     KLGVLYYGDESTQYEPAPVRVDK"
     gene            complement(28141..28818)
                     /gene="radC"
                     /locus_tag="WM481_000573"
     CDS             complement(28141..28818)
                     /gene="radC"
                     /locus_tag="WM481_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFA9079953.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILEEELQEEIT
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQTVRSF
                     RQLGLMP"
     gene            complement(28941..30290)
                     /gene="gshA"
                     /locus_tag="WM481_000574"
     CDS             complement(28941..30290)
                     /gene="gshA"
                     /locus_tag="WM481_000574"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFA9079954.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWNAHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETEKSVLIIPE
                     NHTRNTFYLQNVYALGEIFRSAGCEVRLGSLNPEVTEPAEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGIGQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIDIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGIAD
                     KPFVIVKADAGTYGMGVMSVKSSDEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGVYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            30542..31951
                     /gene="leuC"
                     /locus_tag="WM481_000575"
     CDS             30542..31951
                     /gene="leuC"
                     /locus_tag="WM481_000575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFA9079955.1"
                     /translation="MTAQTLYDKLWNSHVVREEGDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDQNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLNIGGKVPNPAEETDPVKRSGIERALEYMGLKAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGHKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPRQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGHFTDIRTMA"
     gene            32046..32300
                     /locus_tag="WM481_000576"
     CDS             32046..32300
                     /locus_tag="WM481_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079956.1"
                     /translation="MNKLFVTALSALALSACAGTWQGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGKKIENATE"
     gene            32362..33003
                     /gene="leuD"
                     /locus_tag="WM481_000577"
     CDS             32362..33003
                     /gene="leuD"
                     /locus_tag="WM481_000577"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFA9079957.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNGKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAIIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADKIKAFEEKRRQSQPWLFNG"
     gene            33170..34441
                     /locus_tag="WM481_000578"
     CDS             33170..34441
                     /locus_tag="WM481_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9079958.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETLEALGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            34457..35188
                     /locus_tag="WM481_000579"
     CDS             34457..35188
                     /locus_tag="WM481_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFA9079959.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNACPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            35222..>36289
                     /gene="leuB"
                     /locus_tag="WM481_000580"
     CDS             35222..>36289
                     /gene="leuB"
                     /locus_tag="WM481_000580"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFA9079960.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKFIAQGLDADYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEHEGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLAAL"
BASE COUNT         9172 a         8933 c         9033 g         9151 t
ORIGIN      
        1 tcatcccttg cttatcttcc atttgcgaat cctcaaaaac gggcaaaaaa aagccctgtt
       61 acttgtagaa agtaaaaggc gatccgtccg gtttttatcg ggaaggcggc attcatcata
      121 ggattgccgg tattgcgcct gctcgtaccg cctatgccgt acccggcaat caaggctttt
      181 tatcagaatt tttttcggaa taaccctccg gaaaccccat aatcagaagc gtgcgggaag
      241 aaacagccgc cccgccggcg gggttgcggc aatgccgtct gaagccacga atccggcttc
      301 aaacggcatc tgtttaccaa aaggcaaata atccgatttg gcgaaaaaca gatttgcttt
      361 ttggtaaata cgcgcttaca atccgctaca tccgatttct acaaaggata aaacgatgac
      421 cgacacagcc ggcttgcgcc gccgcaacct gcggcaatgg atagcggaac actacggcgg
      481 actgcaaaac cgttttgccg aagccgttgc cctcaacaca ggcgaactct ccgccctttt
      541 gaaaaacaaa tccttcggcg agaaaaaagc ccgcaaaatc gaacaggcgg caaaaatgcc
      601 cgccttctgg ctcgataccg aacacaccgg ccgaccgccc ggacacacag gaaaacacac
      661 catgtcccat atttccccca tccccgaaat cctagccgac atcaaagccg gtaaaatggt
      721 catcatcacc gatgccgaag accgcgaaaa cgaaggcgac ctgctgatgg cggcgcaatt
      781 cgttaccccc gaagccgtca actttatgat caaacacgcg cgcggcttag tctgcctgcc
      841 tatggaaggt gcaatggtcg aaaaactcgg tctgccgatg atgacccaaa aaaacggcgc
      901 gcaatacggt accaacttta ccgtttccat cgaagccgcg cacggcatta ccaccggcat
      961 ttccgccgcc gaccgcgccc tgactattca aaccgccgtt tccccgaccg ccaaacccgc
     1021 agacatcgtc caacccggtc atatcttccc gcttcgcgcc caaaaaggcg gcgtactcgt
     1081 ccgtgccgga cacaccgaag ccggcgtcga cttggcgcaa atgaacgggc tgattcccgc
     1141 cgccgtcatc tgcgaaatca tcaacgacga cggcacgatg gcgcgtatgc ccgaactgat
     1201 gaaattcgcc gaagaacaca agctcaaaat cggcacgatt gccgacctca tcgaataccg
     1261 cagccgcacc gaaagcctgc tcgaagatat gggcgacgcg cccgtacaaa ctccgtgggg
     1321 cgagttccaa caacacgttt acgtcgacaa actctccggc gaaacccacc tcgccctcgt
     1381 caaaggcaca ccctccgccg acaccgaaac cctcgtccgc gtccacgaac ccttcagcgt
     1441 gatggacttc atccaagcca atccgcgcca ttcctggtcg ctgcccaaag cccttgagcg
     1501 cgtccaacag gccgaaagcg gcgtcgtcat cctcctgcac cacaccgaag acggcgcatc
     1561 cctgctcgac cgcaccctac ccaaaggcgc aaaccaagcc tacaaatggg acagcaaaag
     1621 ctacggcatc ggcgcgcaaa tcctcgccgg cctccacgtc aaaaaactgc gcgtcctcgg
     1681 ccaaccgtca tctttcaccg gcctcaccgg cttcggcctc gaagtcgtcg gctttgaaga
     1741 agcggaaaaa taatatagta aattcaaatg ctttatattt gcttcattta ttgcattatt
     1801 tccgtacaaa cgaaaacccg gtctgttggg ttggattttg cttttttcgg atttcgataa
     1861 gtattttcca aaccaaacaa aatgccgtct gaaaggcttt cagacggcat tttaagtttg
     1921 accggtttca ttcatcggta tttatgaatt gaatttcaac atcgccaatc tatccttaat
     1981 ctccttttcc aattcggcag attcggcgaa aagtttatcc aaatccgctg aaaatcccgc
     2041 tattttttgc acaaattcgt cggcggatat atccacataa tcaattttta cctcaaaata
     2101 ctgccccgcc gacaagctgt gattcttcgc tttgatttca tcatagccga ccaccacgct
     2161 gaaatcttct actgcctgtt tgtgcgtgaa ggtatggcag attttttgtt cttcttcgtg
     2221 ggaaagtaca gttttttggt tgtcattaat actaattttt tcacctaaac cggaagcatc
     2281 gattaataca actttatcct tattgacttt atcaataaac aagatagaaa cattggttcc
     2341 cgtcgtagcg aaaatattgg aaggcataga caccacgccg gcgagcattt tgttttccac
     2401 cagatattcg cgaatttttt tatcgatgcc cgattttgcc gtgataaagc cggtcggtaa
     2461 tacaatcgct gccttgccgt tttctttcag gctgaacaga atatgctgga taaaaagctg
     2521 atagatttcc atcttctctt tctttgttgg cttgattttc gggataccag caaaaaagcg
     2581 ttcgtggttt tcatcgcttt ccagccgatc gcggaaatcg ctgaaatcca atttaaacgg
     2641 cggattggaa acaatgaaat caaatttttt cagacagcct gaagcgtctt tgtgggcggg
     2701 cgacaaaatc gtattgccct gaaccacatt attcagcgaa tgcaccagat tgttcaaact
     2761 cagattcagc cgcaacaaat tggacgattt ctgcgaaata tcctgagtat aaatcatgca
     2821 tttatcttcg ccgatgacat gggcgacatt catcaacagc gttcccgaac cggccgacgg
     2881 gtcgtaaacg tccacgctgc ggatttgccc gcgcacctcc tccggcacca aaatatccgc
     2941 cataatccgc gcaaccgcgt gcggcgtgta gtattcggca tatttgccgc cggaattact
     3001 gttgtagtct ttaatcaggt attcaaaaat cgtggcgaaa aagtcgaatt tttgcgcaaa
     3061 aatcgcctcg aagctgaaac ccgccagctt ggaaatcagc gcgcggcaaa aatcgtcgcg
     3121 tcggccttcg tcggtaatgt actgactgat acgctcgaac aacacgattt tcgccccgcc
     3181 ttcggttttc accgaaaaca aatccgcatt acaggcggca atatcggtga gcgtatcgtc
     3241 aaagagtttg gcaaaatccg cgccgttttg ccgctctgat aaatacttga tggaatgttc
     3301 gggctttaaa accgctgttt ttccatcaat atccatattg acgaactcga tcggctcgtc
     3361 gggcttttct ttgcgaattt ttttaacttc aaaatcgtat ttgtcgttta ggaatttata
     3421 caaaaacgcc tgactgatga ttttaaattc gttgccgtca ttgcccaaac catagttggc
     3481 gcagatggtt tttaagctgt cgattaggga tttgatttgt tcggtaaagt gttgttcggt
     3541 catggaatgt ctcggtttgt tgctagcgtc gcttaaattt tgtttagcat tagttggttt
     3601 gctggcagat taaataggtt gaacactatt tgccaacctt ttctcttttc ctaatgctgt
     3661 tgtgtaataa cgttcatcaa taccatataa caaatgtttc agctgtacat cagtagaaat
     3721 ttctacacga gagccatctg cgttaaattt caagatatta ttatattcag caaggtaatt
     3781 taatagttct tgtctttgat ctagcgtcat attattcaat ctatcttgaa ctaaagctat
     3841 tcttttccta ttgggaatag atactttttc acttaaaaaa tcttctttta aatcaataaa
     3901 atcgctttct aagaattgct gtacttcagt atttgtagct tctcgataca aatcttcaat
     3961 ccctttaaat atatttgaaa tagaagaaag ggttctaaat attaatttat catcctttat
     4021 tatataaaca gcatcaggaa tatctttaat aaccaataag tcttgtggtt cgcatagttt
     4081 cgcaccattt ccgcatatag ttagcaagtg cttctttttg agtaataatg aacttgtgat
     4141 tttctggaag aatagtgcat cattagtaca agatactaaa tactttagtt tctctatatt
     4201 tatttcttcc ttttttattt cactaaacat ttttgaatca aataaatttg agttatccaa
     4261 aaacttagga caaaaatctt gattctttaa attttcaatt tgaaaccaag tatcatcatc
     4321 aagattgtaa tcatagttat atgttataaa atttaaattc tcgtaagaaa agtcagggaa
     4381 aatatgatca tcagataata tttttactaa atctttcttg caagtaattt tagctaaaac
     4441 aaaattcatt attgaactcc tatttttcta agaaagtaaa attattaatt cgatatacag
     4501 caatcgaact tacactttgt ggcgttctaa attcctgttt actaatcagc actaataata
     4561 accctgattc agtttttgct tgataaaatc gatagccaaa taagaaaaaa acagggttaa
     4621 aataaaatga cttagagcat gaaactagta agaaaataat tccatacata acaaatagca
     4681 aaaaaagatt attatcgggt atgcttagag caacaaaaaa ataaccaaga tagctaggta
     4741 agaaattatc gttaacatat tctaattctt tcacctctcc ttgcttgaat tcatccttcc
     4801 ccaagtattt gcttagccat aagacagtag cggttgcgat aaatggtatt agaaaataaa
     4861 aaagtagact taacttagaa ataggttcta tccaatgatt tgaaattaca cgataaaacc
     4921 aagtaaggtc taatatataa tattgcgatt taataaaata tattgataac agcaaagatg
     4981 tactacttat atttaataat attttaaata gtttatccat ctcttgctcc gctacatctt
     5041 acggatacct gcccgttcat cagcatgggt agtaggaaat cgcgtagttg ggtcaggtgg
     5101 tggttttgtt ttagattatt tcgaacttta acaaagaaag tatttgctat ttcattatat
     5161 ttttgagaaa tatctttact aggcaaaatt attttaaatt cttttaagaa tttaaaatgt
     5221 ctagcataac cataacttga taaatctatt tgatttatga tttggtaaaa caaatagttt
     5281 ggcattcgtt cattattgct taatattact tgtgtaccat ctgctccccg cgcatattga
     5341 aaattaacta acttaacaat tcttgtatga tcaccaaaaa taatatgagc atcttgtggg
     5401 tttaatatag atttttcatc attggtaaat ccacaaataa aatcttgact ttggtcaaca
     5461 acaggatatt ttccaaaatc cttaatatct ttatttaata tttttgtagt atttgggatt
     5521 tttgctaagc aagattgtaa ttctatactt ccccacccct tcggaatttc ccgtttcaag
     5581 gtttcgtcaa acaccatgtc gccgcctgag gatttgtagg gcttgccgtt ggcgtcgggg
     5641 aaatcgaact gcacaaacca gtagtcgtac agggttttcg ccatctcttc caggcgcgtg
     5701 ttgatttgtt tgttcagggc gatttttttg tccaaagcgg ataggacggc ggcgatggat
     5761 tgttggattt gaggttctgg aataggcaac tccagtgttt ttaatgcatt ttcattgaca
     5821 cgttgcctgc cggaagttcc ttccatacat tcaatcgctc gttttctaaa atcgggactg
     5881 attgcaaaat aatagagaaa ttcgggattg gtttcatttt ttgctcttaa cacgataaat
     5941 tcggtagaac cgaaagccac ttctccatca tctaaaatat ctacaaaagc ggttttccca
     6001 ttttccaaac acggggtaat cttagctaat aaggtatcac cattgcgaaa cttagctcca
     6061 ccgttaaatg ccttgatttc gtaacccgta atttgtcgtt gaaattcttt caacattgcc
     6121 atcggaacac tttttgccaa tgctcccttg gctagttgct ctctcggatt aaaatcagca
     6181 atattttgaa tttgttgttt cttaaaatga ttcatgatgt tccgttacaa aactcttcaa
     6241 aggccgtctg aaaaaaacat ttcatcggta gttcttgccg tctgcggcag ttgtaaactg
     6301 aaagtttaca actgaatcgt ccagttcttg ttctgcaaat atggctttga tgtgtttacc
     6361 gatgttttgt ttgctggttt ggtagagttc ggcaatttct ttttgggcaa gccatagttc
     6421 gccgtcaagc tcgcgcaggg taaattcgga tagtccgtct tccgtggtgt agatgatgat
     6481 gtcgttgttg tgcatagctt tcctgtatct ataaatggtt aaggctacct gaaaatctac
     6541 ttctcaaatc cgcccgcttt cttttaaata ttcccgtacc aacaggcggt tgatggtctg
     6601 gatgtctgcg ggttgaacag gaaattgctg ttccgttcta aagcgtttta atacgatgcc
     6661 ctgcatttgt ttttcaaagt aattctggtt gtccaaaatc tgctccatat ccagcactct
     6721 ttggtcggcg tcggttttga cgccgtttag cgcgttgaag actttgagtt tgtctccgta
     6781 gagggcggcg ttttccatca accgcttgtg gatacgtgcg tatttggcgt cgccgccgta
     6841 tttgtgccgc aagaggtcgt tttgtcggtt cggctctttg attttggtat agacggtttg
     6901 gagtgtggca atgtttgcct gcatttcttc ctgcccgact tcggcgaggt tctttttctt
     6961 gaagatgcgt tccagctctt cgcgcaggct gatgtattca aggtcgtctt ggtcgaagtt
     7021 gcccgccagc ccttcgcgca ctttgcgcat gatgtctttt aaatcgtcgg caagtttgag
     7081 ttccgcctcg ccgattttga cgaattgaaa gtaaacgtct tccagcgctt cattgagcaa
     7141 atgggcggtg tcgccttgct gtaatttttc ggcaaggttt aaggtatcca agcgggcggc
     7201 ggtttcgcgg tacaaaagat tgagcttgtc aaaatccaga tgcgcaagaa attcgtggct
     7261 gccttgcagg cgcaggatgt tgtacaactc tttggcggtt tgcagggctt tttttaaggc
     7321 cagaagctgt tttttgtctt ctatttggct aatttggctg caaaattctt cggcgttttc
     7381 ggtatcgaaa tcaaacaggg cgtttttaat gtctgcaatt tcctgttcga tttcttcggc
     7441 ggttttgaat agctggctgt aactgccgat ttcgtcgccc aattcgttgg aaagttcgtc
     7501 ccaataggcg cggttggttt tgtcaaattc gcgttcgata tcggcaaaat cgacgacata
     7561 gccgtagcgg taagatttgt aggtgcggtt gacgcgggtc agggtttgta aaaggttatg
     7621 ggcttttatc agtctgccca aatagagctt tttcaggcgc ggcgcgtcaa agccggtcaa
     7681 aagcatgttg tacacaaaca agatgtcggt tttgcccgct ttgaaatctt taacccattg
     7741 gtcgcgttct tccttagtgc cgacgtcgtg caatatcaac gcggcggtga aattgtggtc
     7801 tgaggccgtc tgaaaatgct caaaaagctg gcgtgcctgt tctgcgctgt cgcaaacgac
     7861 catggcaccg aggctctcgt cttggttggt ttttctgaat ttggcgaagt cgtccaagat
     7921 gtagtcaagc atcgggtgga cgaagtgcgg atgggcgtag atttctttgc ggtcaaagct
     7981 gcctttttcg atttcaagtt gcgccagtgc ttcttgtaat tgcgccttgt atcggctgcc
     8041 gatttcttcg cgtatcaggc gcagggtgta gccgtcggca atggaggcgt tgtaatagta
     8101 tttgtggatg tagtcgccga agagttcgcg ggtgttgacg ttgcctgcgg ttacgccgat
     8161 aagcggcgtg ccggtcagcc cgattttgac ggcgtttacg tcggactgat taaggttggc
     8221 aagaaatgag cctttggggt tgtagctgcg gtgtacttcg tcgagaaaat agacgcgctg
     8281 aatggcgagg tcgtagtcgt tacgggcgac gacatcgggg tcgtcttgga atttttggat
     8341 gttgacgacg gtgatttccg gtttgcctgc gtggttgtgc agggtttggg cggatttgat
     8401 gtcggcagcg aaggcttcgc ggctgtcgat ggtatggacg attaagtcgc gggcggtaaa
     8461 ttcccgctgc gcctgtttca ataaatcgag cctgtccacg atgaaataga atttcggcac
     8521 gataccctgt ttggcgtaat agtgggtcag gtaacgggtg ttgtaatagg caggcgcggt
     8581 tttccccgat ccttgggtat gccaaatcac gcctttttta ccgccgttcg ataaatgttt
     8641 ttcaatggca agggtggcaa acagttgcgg ataacgcatg atgtgttttt gcaccgaacc
     8701 ttgacttgct ttgacgtaag tcagcccgtg ttgcaggaga aaggcaagac gttcgcggca
     8761 aagcagcgaa gtcaggatgc ggttggtcgg cgtatcgggc gatttattgc tgataaattc
     8821 ggggctgtgt ttgataacgg gcagattatt gtcttgtaga acgttgtttt caaggtcgtc
     8881 tgaaagcgtg tccaataatt cggtaaggtt taatttatgc tcttcgcgga agtagttgaa
     8941 tacgggcttg ccgcaagcag acgaggcgta aaacgcgcct tgtgccggct cggttgcgcc
     9001 gtcgtcgtat tccatgttgt tggaaaaaat catgaattgg gtgatgttga taaaacggcg
     9061 gaatttggga tttttcgcac gtttgcccat gcgctcccgc tcttcgccga tgccgccttt
     9121 attgttgggc tttttgactt cgataaagac caaaggcatg ccgtttacca gcagggcaat
     9181 gtcggggcgg aatgcttcgt cgccgttgat acaaggcaac tcagttacaa cgtggaaact
     9241 gttgttgtca aaattttgga aatcgatcag cttcttaccg ccggattgat tggtgaggcg
     9301 ttcgtaaaat ttttgcccca aatcttcgtt atccagctct aagcgtatgt cggcaagcag
     9361 gcggcgtgca tcatccggcg gcaaatcggg attgatgcgg cagaggctgt ctacaaagat
     9421 ttcgggaaag atattggttt gtctgtccca atttgcattt tttagcgaaa gataatcata
     9481 gcccaagcgc atcaggtgca aaacggcggg gattttgacg cgggaatttt cgttgtgggt
     9541 ttgggacata aacgtaacgt aatgggtcat caaaatttat tgaaacggat tataacacct
     9601 gaaaaaagcc gatacgcctt ttcggtgtac cggctttgcc atactgttct gcttcagacg
     9661 acatcgcttc attttgcctt taatgcctct tcgtccagcg atttcaacca tgccagcttt
     9721 tcgccgattt tgatttccag cccgcgcggg acgggttggt aaaagtccgg ttcgtccaag
     9781 ccgtcgggca tataactttc gccggcggcg taggcgttcg gttcgtcgtg ggcgtagcgg
     9841 tattcgcgtc cgtagcccaa ttccttcatc agcttggtcg gggcgttgcg caggtggacg
     9901 ggcacttcgt cgctggcgtt ttctttgacg aagcggcgca tttggttgta tgccttgtag
     9961 cccgcgttgg atttggcggc ggcggcaagg tacagcacgg cttgcgccag cgcgagttcg
    10021 ccttcgggcg agcctaagcg ttcaaaggtg gcggcggcat cgttggcgat ttggaaggcg
    10081 cgcgggtcgg caagcccgat gtcttcccaa gcgatacgca cgatgcggcg ggcgagatag
    10141 cgcgggtcgg tgccgccgtc gagcatacgg caaaaccaat acagcgcggc gttcggatgc
    10201 gaaccgcgca cggatttgtg cagggcggag atttggttgt agaaactctc gccgcctttg
    10261 tcgaaacggc ggatttgcgc gcccaagctg tcggcgagaa attcggtggt caggattttc
    10321 agacggcgcg tgtcggcggc acgtaaaagt tgttccaaca aattcaataa tctgcgcgca
    10381 tcaccgtcgg cggtattgac gagcagctct tgcacatcgg cttcaatcgt gaaatcccgg
    10441 tattcgggca aagccaacac tttggcaatc agtttcttga ggtcgtctga agacaaggat
    10501 tgcaaaacat atacctgcgc gcggctcagc agcgcgggat tgacttcaaa cgacggattt
    10561 tccgtcgttg cgccgataaa ggtcagcaaa ccgctttcga catgcggcaa aaacgcgtcc
    10621 tgctgcgcct tgttgaagcg gtggacttca tcgacaaaca aaatcgtcgc gcgtccctgc
    10681 tgcaaagcga tttcagcctt atcgattgcc ccgcggatgt ccttcacgcc ggaaaacacg
    10741 gcggaaacag gcaaaaactg ggcattgaaa ctctgcgcca aaatccgcgc caacgttgtc
    10801 ttgcccacgc ccggcggccc ccacagcaac atagaatgcg gcttcccgcc ttccaccgcc
    10861 acgcgcagcg gcttgccttc gccgattaaa tgctgctgcc cgataacatc atcaagcgta
    10921 tgcgggcgca gacgttcggc aagcggtgcg tcgggttctc ggacaaacaa atcggtcata
    10981 acggctccgt caacaggttt tcagacaata tgattatacg gcagggaaag gcggcgtgcc
    11041 gcatacggat accgccccac cgtttgtact aagccgatct taggcgtaca attgggaaat
    11101 ttcaggaaat ttatacgcta tgccatccat gcagctttgc aaccggaccc gcaaagacca
    11161 ggtcccgttt taaccatgcg gggaagttaa gattacagta aacaaaacta ttttgcctta
    11221 ttggaagaaa ggggaacagt tatgagaaat tggaacttat taccaccggg gaaggggcag
    11281 atggagactt actggtcaac ggttcttacg gcatccgttc ttttgcagac ctaaaattgg
    11341 aatacaaacc cgaattatcg gtgagaagca atcatgacat tattttcaac aatgattttc
    11401 aatctgttga cgtacaaccc tattccggaa atatccattt ggatgccaaa ggaaaaattc
    11461 aatttaattt gaatgttgcc gataaggtaa cagatccaac cacacatcaa gaaaagttag
    11521 tgaacgataa cgctttagtg cggtatgtca acaatatcac cccctccgat gaaaatgaca
    11581 aaggcagcat atccttccat tcaaaaacag gcaatttgtt caatatgaac gttaacgttc
    11641 ataaaggcac acaccatttc ggtattcaca acctccgcaa taccaatgtt aatctgactg
    11701 ccgaagacgg cgataatgtg gttgcgatta aagggaagct tgccgagcct atgccggcag
    11761 gaaaagttga gagtacaagt tttttaaggg gtattgaaac ggcttttaaa tccaataccg
    11821 tattaacggg gaataacaat aaaatatcca tcaccactga gggcgacggc ttgggattgg
    11881 gcgtaaatgg cctccagtcc aactccccct attccggaca cacgacgggt actttcatca
    11941 ataatcaacc aagcaatact acccaacctt cccttgacgg caccagcacc atcgaactca
    12001 aagccgtcaa ggggaataat gaagttgatt taaatgtaaa aaaccacgcc tccgtaaaag
    12061 gcattatcac gtcgcattct gcaaaagcaa cgcttgaagc cgaggaggat aatattgtcc
    12121 gggttaaaaa tccggacacc aagacggctt taatcaatgc ctggaaagaa aaatacccca
    12181 ataattcgcc agaaccatac agggtggggg gttcaatcca cgattgacag cgatgtagtt
    12241 ttgagtgcgg caaacaatat cgttgaagta ggaggcagcc cgatgaacca agaagggatt
    12301 accgctcacg gaaatgccac gattaccctc aaggcgaaag aaaacaataa aattaccgtg
    12361 gaaaacgccg catacagcag cgacggcatt tcgactctga ttaacagaac gggggcaaga
    12421 cccggaacaa gggatgatgg aaataaaatc atactggaag ccggcggcga taatattgtt
    12481 accatgaaat ccggcgatgc ggatgcggat tatgtaaaca attccaaagt attaacggag
    12541 acaccatatt ataaaagcaa acgaggttcc aacggcattt ttgcctatgg cgacaaatcg
    12601 ctggtcaaac tgattggcga gaataatatc gttaagagtg aaatcagtga aaaatctaag
    12661 gcattaaatg ggggatttcg ccatatcggc atttattcat ggcaaaacgc gaaagtcgaa
    12721 ttgtctgcga agagcgacaa tatcgtacaa ggcggaattt ggggcttata ctccaacaac
    12781 tcctcaattt ccctcaaggg gaaaaaataa tgtgatttca aacccgaaat ataatgtttt
    12841 cgcctacaaa aaggcaaagg tggatttggt caaattacca ttgactccaa cgttgccaat
    12901 cttgcaaggc aagatggttc aattcatttg aattataaag acgatacccg tatcacaggg
    12961 gcaaccgtat ctgataaggg tttggtagcc atcaaacctt tgaataacac gaatattgtt
    13021 gccgacacta ttcactataa aggcgatgtc ttggcggtaa ataagggtaa agtggaatta
    13081 gatttcacgc cgaacatccg tttagtggga cgtttggata attttagcgg cttaaccgat
    13141 tccaaacata aaaatttatt cgaaaactat gttgcaaatt tagacagcaa aagtgcgggc
    13201 gaaattaact ttaatttagc caaagacgca ttatggacga tgacaggtca aagctggctg
    13261 gataaattgg aaggacaagg cactatcgat tttaataatg atgctaaaac aagtggacgc
    13321 gccttacata tcggtgaatt ggcgggtgcc aataaattct tgatgcatct gaataaagac
    13381 ggcattcaca gcgatatgct ctatgtgaaa aaaggcactt cgacaccgca agaagtcgtc
    13441 gtcaaaaatc tgtccgaagt gctcgacagt atgaattacg gcgaacgttt gcgtttcgct
    13501 acagtaacaa actcaaaaaa tgaatttgtg aacggtaaaa aatatattga cgatacgcac
    13561 cttatggagg atgccctgac tgtcgaatac tccgcacata acggcgataa aaacaacaag
    13621 gatgactata ataaatcctt taacggctct gaaatgacgg cggaaaaagc tggagacgat
    13681 tatgtcaata aaacctatac cgacaacagg caaaatgtct atttggtcaa acaggctacc
    13741 ggcaatccga gccgaaatgt caaaaatatc aatgatatgt tcgattcaac cgcacattat
    13801 gcgttcactt tggatactta tgccaaacgc gaaggggagc gggctttttc aacgttggat
    13861 aaaaaagaag gcgattggat aaggctgacg catacccgtg tgattcaatc caatgcgttt
    13921 aggtttcata acaacgattt tgaaatcgga tatgaccgat tcagcctcaa cgagcaggag
    13981 aaaaaacgca aatggggcat aagtctcgac tacggccacg gcaggacatc attatggaat
    14041 acgtttggca aggacaaaat caggaaatat gaattggctc tgtacaatac tacccaatac
    14101 atagataaag aaggagacga aacagggtat atcgacaatg tattaaaaat aggaaaactg
    14161 cgtaaccgtg tgattgcacg aaatcatatg gggcaattat ggggcaaggg aaaatatagc
    14221 aacaccctat tctctatcag caccgaatac ggccgccgta aatttttgga tgacgataaa
    14281 ttgtggcgga ttacaccgca agtacagttg caatattcct atttgagagg taccggctat
    14341 cggatcgata acggcataaa cgtcaattta agccacgcaa acagcctgat aggccgcttg
    14401 ggtttggatg tcgtgagaaa atttgacgga ggcaaaaaac ttttctatat caaaggcaat
    14461 atctttcatg aatttttggg cagtcgttcc tttaaggcat ttgagggcaa aagtcattat
    14521 gctcaaaaat gaaatacgag ggggacttgg tattccgccg gattgggcta caatgcgcgg
    14581 acaggcaaaa aaaacgaatg tctttgccga tgcggaaaaa agagttttcc ggcgggaaaa
    14641 aaggatcgta caacatccgc ttatccgttt cccatcagtt tgactgaatg atatcggggc
    14701 cgcgccatta ttcggcaggc cccattttat tgccacacat tgcctgcccg attcccgttt
    14761 caacagcaaa cccttccaat attttgttca aacttggatc gcggcagatg ggggcgtgca
    14821 agccccgcaa ggtttttttg gcttttacgg aacagcacgc atttcgccaa acggcaaaat
    14881 tgcattaacg gaattgaaaa cttttggaat tgtgcaaaac ggcatttgcg tcagtttaac
    14941 ggcatttcca aagggcattt taagccgtat tcgagagaat gcggatggcg ttttcagtgt
    15001 tttcagcgtg ataaaattca aatttctatt ctaaaacaat cagcaaagca ggatttattt
    15061 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaacgcac
    15121 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
    15181 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
    15241 tcggctttgt ttccctgcac cattgccaca cctataccgc ctgcaaccaa atcgataagg
    15301 gtttgggggt agtcgcacaa tatctgtttt tttgggtgag agccggttgc tgcgccaaaa
    15361 ctggtgcagg tgcttcctac ttccggacac gcccgacatt tctatccata cgcattcttg
    15421 caggctctgc ggaagggagc gggtcagccg ggaatattgg ctttgcgggc aaatcagcgc
    15481 gtaggtcagg ttttgcagga atacgctgcg gatgccgcct tgggcggcgt tgccgagtat
    15541 aaagccgccg tgcagggttt tgtgttggat gcgcgagagg atttcgccgc tcattccgta
    15601 ttggatgtgc aggcgcgttt tggggggctg tttgaccgat attgtccgtc agcgcgacga
    15661 gttttgccga atcgatgggg tggataatgc ccaaactggt ctcttccgaa taatcgcctg
    15721 ccagcgtttt ggcaaaatac tccaacttgt gtttgtattg cagcagggat tccgcttcgg
    15781 gcaacagtat ttcgcccgcc cgcgtcaata ccatgccttt ccccgtgctc ctgaacagcg
    15841 gcgtgccgac atattcttca agggctttaa tttgggcgga aacggcgggc tgggaaagga
    15901 aaagtcgttt ggcggtttgg gtaaggttgc cctcgtgcgc gacggcgaca aatgatttta
    15961 attgtacggc atccatgtat ccctccttgt gcggatgttt tctatatttg tgcaatcgaa
    16021 atcttttagg cggattgttg ctgaaaatta actttttaat caagtggttt gtaaattgta
    16081 tcagttttcc ggatgatgat tattaaaaaa aaattggttt tattgcctgt ttgggcttta
    16141 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccccttt
    16201 tacgaggcgc gatatataag gaggaaggtt atggaaaaac ataatgggac ttatcgggat
    16261 ttacaccgtc cggcttcgga atttgcgacg cgggacgaat atttggaaca tgaattgcag
    16321 attatgcaac caaaacgctg gcggcccaac ctgccctttc gcgattaccg cttcgagtgg
    16381 gaggatttga ttcctgcgat ggcgggaacg attggaaaag tggtgatggt gggggggcgg
    16441 cggcgtttgc cgcacctttg gggctgcctg acagctttgt actggaaaat gtgcgctatg
    16501 agcttttaat cgccgccgcg tttatcttat tggtatcggg cttttttctg cccggcgcca
    16561 acctgcccgg tacgcacggg ccgctgattc cgatgattcc catcgttgtg tcggcaggcg
    16621 ggcatccttt ggcgttcggc atttcgattg cggttttagg tctgctgatg gctttatttc
    16681 gcggcggcag tattatggcg aagctgacaa gcaacggcgt atgcggcgga ttattactct
    16741 atttgggctt tatcggcacg acggggcagg taaaaaaatt gttttcgtgg gcaggcggtt
    16801 ttaatatgcc ctacatcgct tttaccgtca ttattgtaac gattgtgatg tacgctttgt
    16861 tggagcattg gaaaaaacgc tggttggccg tgcctttggg atgcttgatt gccggtgtgg
    16921 cggcatttgc attgggtgcg ccgtttgagt ttcacaccgc cccggcctgc ctccaatgag
    16981 tcctgcttat tggtagggtg aaaacagcgg ctggcatctg gggttgccga cggcagaaag
    17041 ttttttggtt gtctttccat ttgcggtatt ggctgttgca atgtggtcgc ccgatttttt
    17101 aggacatcaa gtgttccaaa aaatcagcta tccggaaaaa accgataagg tattgatgaa
    17161 tatagacgac accatgacaa gttgttctgt ccgtcaagca gtgggttcta ttttaggggg
    17221 tgcaaatttt acctcttctt ggggaactta tatcgtgccg gcatcgattg ccaaacgccc
    17281 cattccgggc ggtgcggttt taacggcggt tttatgtatt atcgccgggt tgtggggcta
    17341 tccgatggac ttggcgattt ggcagccggt attgagcgta gccttgatcg taggcgtata
    17401 cttaccgctt ttggaagcgg gcatggaaat gacgcgcaaa ggcaaaacca cccaatccgc
    17461 cgccatcgtg gtgttctctt ccgtctggtc aatccggttt tcggctgggc gttgacgatg
    17521 ctgttggata atttgggctt aatcggctgc aaagaacgca gcgcgcaatt aggttttgtc
    17581 ggacgagtat tgatacccgc agtaggtttc ttaatcttgt gtgtggcgat gggtgcggtc
    17641 gggatgctgc ccggtatccc tccgtttttg gagcagttca aatctttggg ctaggctgaa
    17701 atcgcaaatg ccgtctgaac cggtttcaga cggcattttt gcaaacaggc aaaatgacgg
    17761 cggcgggatt ttttattttc ccgattgaag tataatgttg ccgggcttca accggatatt
    17821 caaacggttt gctccaacac tcggaacggc gcataaaacg ccgcccttcg cgttatcccg
    17881 aacggggcgg ctaatcagat cctatcgcca taaaaggcgg ggtttcaacc gaaaaggaat
    17941 tgagatgaat aaaaccttgt ctattttgcc ggcggcaatc ttactcggcg ggtgcgccgc
    18001 cggcggcaac acattcggca gcttagacgg cggcacgggt atgggtggca gcatcgtcaa
    18061 aatgacggta gaaagccaat gccgtgcgga attggacagg cgcagcgaat ggcgtttgac
    18121 cgcgctggcg atgagtgccg aaaaacaggc ggaatgggaa aacaagattt gcggctgcgc
    18181 taccgaagaa gcacctaacc agctgaccgg caacgatgtg atgcagatgc tgaaccagtc
    18241 cacgcgcaat caggcacttg ccgccctgac cgtcaaaacg gtttccgcct gcttcaaacg
    18301 cctgtaccgc taaccttctt cagacggcat gaggcagatg ccgtctgaat ccgttttcct
    18361 gcaatttcga caaacgagaa aaatcatgag atacatcagc acgcgcggcg aaaccgcaca
    18421 caaaccgttc agcgaagttt tattgatggg gcttgcaccc gacggcggac tgatgctgcc
    18481 ggaacattat ccgcaaatcg ggcgcgaaac cttggacaaa tggcgcggtt tggcttatcc
    18541 cgaattggcg tttgaaatca tgtgcctgtt cgttaccgat attccggagg acgatttgcg
    18601 cgacattctg aaccgtactt acacggaagc ggcgttcggt actaaggaaa tcacccccgt
    18661 ccgcacgctt tcagacggca tcaaaatcca agccttgtcc aacggcccga cgctggcgtt
    18721 caaagatatg gcgatgcagt ttttgggcaa tgcgtttgaa tatgttttaa acaaagaagg
    18781 caaaaaactc aatatcttgg gcgcaaccag cggcgatacg ggttcggctg cggaatatgc
    18841 cttgcgcggc aaaaaaggcg tgaacgtatt tatgttgtcg cccgacggta aaatgagcgc
    18901 gttccaacgc gcgcagatgt acagcctgca agacgggaat atccacaata tcgccgtgaa
    18961 ggggatgttt gacgactgcc aggacattgt gaaggcagtg cagaacgatg ccgcgttcaa
    19021 ggaaaaatac catatcggta cggtcaattc gatcaactgg gggcgcatcg tcgcgcaagt
    19081 ggtttattac tttgcaggct atttcaatgc cacttcaagc aatgacgaaa ccgtcagctt
    19141 ttgcgtaccg agcggcaact tcggcaacgt ttgcgcggga cacatcgcca aacaaatggg
    19201 cctgcctgtc cgctgcctga ttgtcgcgac caatgaaaac gatgtgctgg acgagttttt
    19261 caaaaccggc gcataccgcc cgcgcaacag cgcgcatact tatgtaacct ccagcccgtc
    19321 tatggacatt tccaaagcgt ccaacttcga gcgtttcgtg ttcgacctga tggatcgcga
    19381 tcctcaggaa atcaatacgc tgtgggcgga agtcgcggca ggcaaaggct ttgacctgcg
    19441 gtttgccttg gacaaagtcg gcggcaaata cggctttacc tccggcaaat ctacccacgc
    19501 cgaccgcctc gccaccatca gacaggttta cgagcaagac aaagaactca tcgacccgca
    19561 tactgccaac ggcgtaaaag tcgcccgcga agtgcgcgaa gcgggggaaa cggtggtttg
    19621 tttggaaacc gcgttggcgg cgaaattcga tgcgaccata cgcgaagccg tcggcgatgt
    19681 cgccattccg cgccccgccg cgctggaagg tttggaaaac ctgccgcagc gcgtgcaaac
    19741 cgtgccgaac agtgcggatg cggtaaaagg catcatcgaa caaacccttg cctgatgttc
    19801 cgtatgggcg gcattccatc cgaacttaag attgaaagaa aatgacctta cctatgcagg
    19861 aaacccgttt ttctatactg ctggacgaat tggcggcaaa acaggaggca accatcgccc
    19921 cctacctgct tgccgacggg accaaggtat gggtacgcaa agcaggcagg cacaatgcgc
    19981 gatggcgtta cgcgctgctc ggtatggttg cccgatattt gaaactgggg gtgttgaagc
    20041 cggttcccag cctcggcggc gagcctgcca ttgcaaccga atcaaaacgc ctgtacgaat
    20101 tgcgttcggc agggatagcc gttcccgaat tgctcgccgt ccggaaaaat gcccttatgt
    20161 tcggcaattt ggaaggcatc ccgctcgata cgcaaatccg ccaagaagcc gaagccggaa
    20221 aggcggacgc ttggcttgcc ggtttggaag ccattgcacg cgtgcataaa aaacggcagt
    20281 tcctcagtca ggcgtttgcg cggaatatga tgtgggacgg aaaaaacatc agctttttgg
    20341 attttgaaga cgacccttcc gaggtcctga ccatagcgca atgccaagcc cgcgattggc
    20401 tctgctacat ccattcgacc gcgctgatat tgaaaaacgg cggactgctg gaagcggctg
    20461 cggaaaaatg gggcggcgta ttgtcggatc agcctgccga aatacaaaag ctgattgccg
    20521 gcaccgtcaa accgatcctc ccgatacgca ggctggaaca cccccgctgg ggacgggacg
    20581 cgctcaggct ggcagcctcg atttccttaa tttccctcgc cgatatgccg ccgtaagcca
    20641 ccgcccgccc cacgatgccg tccgaagccc ttcagacggc atataaccaa cgcgcatcag
    20701 gcattgtcgc aaccatccgt ttcgtttaga attttgactt tatcccgaat cgggcagaca
    20761 tttccactct gccgcacgtc ccgaacaagc actatccatt tcttttactg acaggaaacc
    20821 caaatggcag cattcaatac ccaaaaagta ttgtccgtac accactggac agacgcatat
    20881 tttaccttta cctgcatccg cgacgaatcg ttgcgctttg aaaacggaca gttcgtcatg
    20941 gtcgggctga tggcggacgg caagccgctg atgcgcgcat acagcgtcgc ctccgccaac
    21001 tgggaagaac acctcgaatt tttcagcatt aaagtccaag acggcccgct gaccagccgc
    21061 ctgcaacacc tcaaagtcgg cgacgaagtg ttaatcagca aaaaaccgac cggaactcta
    21121 gttgcctgcg atctgaatcc gggcaaacac ctttacctgt tgagtaccgg taccggcatc
    21181 gcccctttct tgagcatcac caaagacccc gaaatttacg aacagtttga aaaaatcatc
    21241 cttgtacacg gcgtgcgcta caaaaaggat ttggcatact acgaccgctt taccaaagaa
    21301 ttgcccgaac acgaatacct cggcgacttg gttaaagaaa aactgattta ctacccgatt
    21361 gtttcccgcg aagaattcga acaccgcggc cgcctgaccg acctgatggt aagcggcaaa
    21421 ctgtttgaag acatcggcct gcccaaaatc aacccccaag acgaccgtgc catgctgtgc
    21481 ggcagccctg cgatgctgaa agacacctgc aaagttttgg acgatttcgg tctgacagtc
    21541 tccccgaaaa ccggcgtgcg cggcgactac ctgattgagc gcgcgtttgt ggatcaataa
    21601 gccgcttgaa aatgccgtct gaacggcatc agcgataaaa aaacccgaat cctgccaatc
    21661 aggattcggg atttttatag tgggtaaaaa ttagaaatgc acatttccgt cattccaaca
    21721 caggtgggaa tccggactta cctgtttcat tatcgtttga aaataaaaga gaaaccaaac
    21781 atatagattc ccgcttgcgt gggaatgacg acggtgtttt gccgtagcca aagcaggaaa
    21841 acatccttat tgtcatttca tcatgatagg acagtaagga tgttaaaaat taattatcat
    21901 tatatgattg atatataata aaaattatcc caataagaaa tgttatgcag aaaataaaat
    21961 gaaaaggatg gttttcattg aaaatggttg ttctgccacc gtcatacaca gaaccggtat
    22021 ttggaaaaaa aaataatcca tattgacatc attattccat ttatcgcata aatccacttt
    22081 ttaatttttt tcctcattaa tataatctct tatttaataa aatcaaatac ctagcaattc
    22141 gatctcaaat tttaaacgcc aacgtgtttg agcggtaaca atgcatactt taagtttgta
    22201 atccttcatc gtctcatttt tgttttaatc gactatacgt ctaaagcaac gccgcccgtc
    22261 attgttcgga cagtattttt tcagacggca ttttcccccg tttgtgaagg cgcaaggcat
    22321 tgcccaaaac cgccagcgaa ctgaaactca tacccagtgc ggctatccac ggttggacat
    22381 agcccaaaac ggcaagcggt acggcgatga tgttgtacgc gcccgcccat atcaggtttt
    22441 gccggataat atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat
    22501 cttcgttcaa taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg
    22561 cggcggatac gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca
    22621 gcactttttt cccttctttt tgcaaggctt cgacgtattc cagcttgtct tccggcattg
    22681 cttgggcgcg gtagtgcgcg atacccaagg cacgggcggt ttcagcaact gcttcttcac
    22741 ggtcgccact gaggatgtgc agcgtcaggt ttttgcctgc caactgccgc actgcctccg
    22801 ccgcgctgtc tttgagcggg tcttgcaggt agaacacggc ttggaaaccg ctttgactgc
    22861 cgaggtaaac cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcaa
    22921 cataggatgc cctccccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcac
    22981 ccacgccttc cccgatgcgg ttgaggcgtt gtccgacttg gatttcgggg acgctgccgc
    23041 ctgaaatgcg gcagttgaga atggcgcgcg cgagcgggtg ttcggactgc tgttccaaac
    23101 tttgcgccac tgccagcact tgggcttcgg tcatgctgcc gagcagttca atccggcgga
    23161 cggcgggatt gccttgggtc agcgtgccgg ttttgtcgaa aatgatgtcg gtggtttggg
    23221 agagggtttc gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc agcgtaccgg
    23281 tagaagctgc cagcgcggtc ggcgtggcaa gcgacagggc gcaggggcag gtaatgacca
    23341 gcagggcgac ggtaatccac aatgcggtgt gcgcatcggc gtacagcgtc cagccgatga
    23401 agacggggac ggcaagcagg agctcgccga atatgaaaga tgaggcgtat tgttccgcca
    23461 actcggcagt acgcggtttt tgcgccaagg cgcggtcgag caggcggacg atgtgcgaca
    23521 ggcgcgtgcc gccgccggtg cggtcggtgc gtataatcag agggctttgc gtgttgagtg
    23581 tacctgcggt tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca
    23641 tagattcgtt gacggcactg tttccttcca gcaccgtgcc gtcaacgggg atggtttcgc
    23701 cgggtttgac catcacaata tcgcctgctt ggagtttgac gacggcggat tcgcggacat
    23761 cctgtacggc aggatagccg ggcatccggt ggcagaaggc gggtatcagt ttcaccagcc
    23821 tttcggcggc atcgcctgcc ttgcgtcggg caatgtgttc cataaagcgt ccgcccagca
    23881 ggaaaaacag cagcatcgcg atggattcga aatacatccc ctgccctgcg ttggtggcga
    23941 ggctgtaaat gccggcgatg aaggtcatga tgatggcggc ggcaatcggc gtgtccatac
    24001 cggcgcggcg gtttttcagg tcgcgcaacg cgccttgata aaacgggacg gcgcaataaa
    24061 atacgacggg cagcaccatc aggaagccgc cccaatggag gatctgcagg aaatcgggtt
    24121 cgatgtcgcc gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcataccca
    24181 gtccggcaac ggcaaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga
    24241 ttttttgcgc gtcatagggt gcggcggtgt agcctgtctg ccggattttc aacagaatgt
    24301 cggagaggcg gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga
    24361 ggtcgatgcg gacgatgccg tctgtgcgca aaagctgctg ttcgatcagc cagacgcagg
    24421 cggcgcaggt aatgccgctg agcatcagca ctgcttcgtg cgtgccatta tgggtttcca
    24481 caaagtcgga ttggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg
    24541 gcggcagttc ggtttttttc gcgtcggcgg tgcgtcgttt gtaataactg cccaagccgg
    24601 aatcgatgat gctttgtgcg actgcctgac agccgacgca gcaggtttcg cggtcttcgc
    24661 cttcgtagcg gacggtcaga tgcaggtttt cgggaacgtc cagcccgcag tggaaacagg
    24721 tttttttcat ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaat actcggcaat
    24781 ggcttcgcgc agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat
    24841 ttttcccgca gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt
    24901 tttgttgtac accttgatgc acggaatatc gtgggcatgg atttcttgca gtacgttttc
    24961 cacgtcttca atctgctgtc cgctgttcgg ggcagcggca tcgacgacgt gcagcagcac
    25021 atcggcttgc gcggtttctt ccagcgtggc ggaaaatgcc gaaatcagtt tgtgcggcag
    25081 atcgctgacg aatccgacgg tatcggtcag gataatgctg catgcgggac tgatgtacag
    25141 ccgccgcgcc gtcgtgtcga gagtggcgaa aagctggtct ttcgcatata tgcccgactt
    25201 ggtcagccgg ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt
    25261 tttgattctg cccgactcgc gggacttgcg gcgcagggcg cgctgttttt tgaggttggc
    25321 aagctgtttt ttcaaggcgt tgatccgatg ggcggttaat cggcggtcgg tttccagttt
    25381 ggtttcgccc ggccctttca tgccgatacc gccgcgctgg ctttgcaaat gtccgtaacc
    25441 gcgtatcaag cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc
    25501 ctgcgtgcgg gcgcggcggg cgaaaatcgc cagaatcagc cccactctgt ccaatacgcg
    25561 gcattggagg attttttcca aattgcgttc ctgcgtggga gtaagttcgt ggttgaatac
    25621 gaccaaatca atgccgtctg cggcaactgc ttccgacagc tccgccgcct tgcccgtgcc
    25681 gacaaacagc gcagtgtgcg ggcggtcgcg tttggcagtc tccacgcgta cggaatcgcc
    25741 gcccgccgct ttgaccagct cgacggcttc cgccaatgcc gtctgaaaac cgttcagacg
    25801 ggcggcattg ctgcccgtat catctttatc caacattacg cccaccagca tgacgcgttc
    25861 cggttgcgcc tgagtggcac tgttccgtcc tgttctacct gacataactg tcaatcctgc
    25921 aaataaaggg ttacaccgcg ttccgggctg tcttccggag taagcctgat gcggcttccc
    25981 gccattccgc tgacgctgaa gacaatgccg cccgcttcgt gccggagtat ggacaggctg
    26041 ttgccgaact gccgccggta ggtataacgg gtacacgctt ccagacacca gcgtccgcgc
    26101 cagtggatga aatatttttc gccttttcta accgcaagtg tctcgccgcc gtttacctca
    26161 aaataagggt aatcgggttt tcgggttcta aactgccatt tcgcctgcgc gcgccgtccg
    26221 ttgcggacat agtcgaatac cgccgtataa agcgtgccgt attccaaagg tttgagcggg
    26281 aaaagggcga attggtgcgc ggtcagcctg ccgttagggt cgttgccggc ggttaaaacc
    26341 ctgacggggc ggatttcgtt tttaccctga tacagcttga aacttttcat cgcaattttg
    26401 cctgccgcct cggaaaaatc aatgctggca ggattgcctg tgatttcata ttccggcacg
    26461 gggtcgggac gttccccgta aaaataaggc agcgcgccgc cgccaaccgg ataggcggta
    26521 taaagcaatt ccgttacggg catggcttcg tcagcataaa cggccgcacc gttgtggcaa
    26581 gcgttgcggt aatatttccg tcctgcttcc ggctgccgcc ttccttttgc acaggcgcgc
    26641 tcgaagctgc cgttgccctg attgaatatg aggacggttt tgccgttttc gcgcacaaat
    26701 gccgcacctg cttcgtcggt atggcggtca agcagcgaaa ggcggtgata gacagcactc
    26761 atcaaagcgt ccacttggcg ttgctgcgtg cggatgtcgc tgtcggacga ttcggcggct
    26821 tcctcttccg tgctgatgtt ttcatgcacg ccgttgtaga gatacccggc aaggcgtgtg
    26881 cgttcggtca gcttttgtgc ggtgtagtgc ggattgtcgg gatggtgttc gccgtgtccg
    26941 tcttcgggat tgagcgtgag atagcgtgcg tgcctgcggg cggaattttc caaaaccggc
    27001 gcgtgtgcca gcgcgtgcaa accgatttgt gtgcggatgc ggttgaggta gtgcagcgca
    27061 tcgccgccgt ccctgatttg cggtgcggac ggatagacaa gttcgcccgc gggcagggat
    27121 tggttttggg tatggtagaa aacgcctgcc gccgagccca atagaagcag ccaaataaaa
    27181 agggatttca taatgccgcc ctcccgacgc ggcaacgggg tgttttatca aaacggcaaa
    27241 cttgccaatg cggtttccgc ttgccgattg ccaaaccggc aaacctttcc cgtgccggtg
    27301 ccgaacccga tgccgtctga accttcagac ggcatcgggt gtttatttgt ccactcggac
    27361 gggtgcaggt tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgacggg
    27421 gatgcgtccc tgcgatttgc ggtgggcgtt ggaatcgcgc aaggaatagg cgcagccgca
    27481 gtattcctgc tggtagaagt tttcgcgttt gctgatttca atcatacgcg cgccgccgcc
    27541 gcctttgcgc cagttgaagt cccaataggc cacatcatcg taaggcgcgg cggcacggtg
    27601 tccgcagtcg ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa
    27661 gacgggaaaa ccgtgttcgt gcgcgtattg cgccgctttt tcaaaacgca tatcgaaaca
    27721 catggtgcag cggatgccgc gttccggctc aaactccatg cctttggctt tggcaaacca
    27781 ttctttgcgg tcgttttcgt agtcgtcgtc tttatcgatg aaagggatgc cgaacttttc
    27841 cgcaaagcgc atgttttcct cttttcggag catatactct ttgtgcggat ggatattggg
    27901 attgtaaaaa taaatggtgt agtcgatgcc ggaggcaagc atggcttcca tcacttcgcc
    27961 gctgcacggg gcgcagcagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag
    28021 tatggggcgg tcaatgtcgg taacggtagg tttgttttgt gtttccatta acttaacaat
    28081 atctgtcgaa tacggtcaaa gcggctattt taccagagcc gccgaatgta aaacagggtg
    28141 tcagggcatc agcccgagct gcctgaacga acggacggtt tgcgaggtaa cgataaaatg
    28201 gtcgagcagc gaaacatcaa ccagcgacat ggcctgtgcc agccgccttg tgaacatgat
    28261 gtcttcctgc gaaggttcgg gcgagccgcc cggatggttg tgcgcgataa tcaggctgtc
    28321 ggcatattcg tccaatgcca gtttgacgat ttcgcggatg taaatcgtgt tttccgcaac
    28381 cgtaccgcgc gacagctctc tgaccgcaat cagttggttt tggcggttca gcagcagcgc
    28441 gacgctgact tcgacttttt cctgccccaa atgaaagcgt aaataatcgg caacggtatc
    28501 gggatcggat aaggtaattt cttcctgcaa ttcttcctcc aatatccgcc gcccgatttc
    28561 cctgaccacg gcgaactgtg tgaaacttgc cgtccccatc cctttgtatg ccgacagttt
    28621 gccgacctcc gcgctcatca gcctccccaa gctgccgaac tcctgcaata ggtagcgcgc
    28681 caaatcgacc gcgctcatcc cgcgcgtccc gacgcgtaaa aggattgcca aaagttcggc
    28741 atcgctcaaa gccgccgccc cgcgttccaa cagcttttcc ctgggccttt cgccttccgg
    28801 ccattgcttg atgctcatgt tttccctttt ttaaatagtc tgagatgccg tctgaaacag
    28861 atgttcccac acgccaaata tccgaaatgc cgtctgaacc ggatttccgc ttcagacggc
    28921 atctgtattt caaaacagtc ttacgccgtt tcttccaact cgattgaagc cgccaaaagc
    28981 gacagacgtg ccattacgcc gtaaacgtag aggcggttat gttcgttgtc cacgtcgcag
    29041 tctttttcag aacgcggcat accgagcgaa tcccattgtt cgaatacgcg tttgcaggct
    29101 tctggagctt cttgcgaatt atcgccgtta ccggtaggaa tgctgttgga cagcggcaca
    29161 aacaccatac cgccggcgtt gaggttttcg tccgcaccgc gcccttcgtg tacgcggaaa
    29221 aagccgccga tgacgaaacg gtccatcata tagacgacgg gttcgcacac cgcgccgttc
    29281 aaggtttcat aggtataaac cccttcttgg acaatcactt cgctgacttc caagccctct
    29341 ttcaccttcg ccattttgtt gcggtttttg cggttcaagc cgcgcacttc gtcggaggat
    29401 ttgacgctca tcacacccat gccgtaagtg ccggcatcgg ctttgacgat gacgaaaggt
    29461 ttgtcggcaa tgcccgattc gtcgtatttg gcttgaattt tcgccagcac gcgttctacc
    29521 gcttccgcca gcgcatcttc accttcgcgc ccttggaagt ccaaaccgcc gattttttca
    29581 aaatacgggt tgatctgcca ttcatcgatg tcaatcagct tggcaaattc ggcggcaact
    29641 tggttgtacg cgccgaaatg atttgttttg cggcgcgtcg tccaaccgcc gtgcagcggc
    29701 ggcaaaacgg tttggccgat gcctttgaga atgtcgggaa tgccggcgga caaatcgttg
    29761 ttcaacaaaa ccacgcaagg cgaaaagccg tcggcaaggt ggacgcggtc gcgggttcgc
    29821 agcaaaggct caagcaggat tttgtcgccc aatgcagttt caaattcggc cggttcggtt
    29881 acttccggat tcaggctgcc caagcgcact tcacaccctg ccgaacggaa aatctcgccg
    29941 agggcgtaaa cgttttgcag gtaaaacgta ttgcgcgtgt ggttttcggg aataatcaat
    30001 acggatttct ccgtttcgca ggcgcgttgc accgcatctt gcgcggcaac cgccgccagc
    30061 gggatgaaat tcggattcag attattgaag ccgcccggaa acaaattcat atcgatagac
    30121 gaaattttgt aaccggcgtt gcgtatatcg accgaaccgt aaaacggcgg gcggtgcgcg
    30181 ttccactgcg tgcggaacca cgcctcgatt ttggcgtgat tggacaggat ttttgcctca
    30241 aacgcctgaa gttgcgccga atgttcgggc gacataaccg gtaatttcat ctttcgtctg
    30301 cctctgtgtg ttttttgaca aggtacagat aataatgctt ttgaaagaat gacgacaagt
    30361 attctttcgt atcgaatcat tctttagata taacgaaatg aacaatatgt ctaatttttt
    30421 cgttaaaatt gtttacaaaa taacaccgac tcaaaaatta gacaaaatct gttgcgcagt
    30481 ataaagaata cgtctaaaat ccgcccaatc cgcatcacca tttatccaaa agaacaacat
    30541 catgacggca caaaccctct acgacaaact ctggaacagc cacgtcgtcc gcgaagaagg
    30601 agacggcacc gtcctgcttt acatcgaccg ccatctggtt cacgaagtaa ccagcccgca
    30661 ggcgtttgaa ggtctgaaaa tggcggggcg caagctgtgg cgcatcgaca gcgtggtctc
    30721 caccgccgac cacaacaccc cgaccggcga ttgggacaaa ggcatccaag acccgatttc
    30781 caagctgcaa gtcgatacgt tggatcaaaa catcaaagag tttggcgcac tcgcctactt
    30841 cccgtttatg gacaaaggcc agggcatcgt acacgttatg gggcccgaac aaggcgcgac
    30901 cctgcccggg atgaccgtcg tctgcggcga ttcgcacact tccacccacg gcgcattcgg
    30961 cgcactggca cacggcatcg gcacttccga agtcgaacac acgatggcga cccagtgcat
    31021 taccgcgaaa aaatccaaat ctatgctgat tgccgttgac ggcaaattaa aagcgggcgt
    31081 taccgccaaa gacgtggcgc tctacatcat cgggcaaatc ggcacggcgg gcggcacggg
    31141 ttacgccgtc gagttcggcg gcgaagccat ccgcagcctt tcgatggaag gcaggatgac
    31201 cctgtgcaat atggcgattg aggcaggcgc gcgttccggt atggttgccg tcgatcaaac
    31261 caccatcgac tatgtcaaag gcaagccctt cgcacccgaa ggcgaagctt gggacaaagc
    31321 cgtcgagtac tggcgcacgc tggtgtctga cgaaggcgcg gtttttgata aagaataccg
    31381 tttcaacgcc gaagacatcg aaccgcaagt tacttggggc acgtcgcccg aaatggtttt
    31441 aaacatcggc ggcaaagtgc cgaatcccgc cgaagaaacc gatccggtca aacgcagcgg
    31501 catagagcgc gcactcgaat atatgggttt gaaggccggt acgccgctga acgaaatccc
    31561 tgtcgatatc gtctttatcg gctcttgcac caacagccgc atcgaagact tgcgcgaagc
    31621 cgctgctatc gccaaaggcc ataaaaaagc cggcaacgta cagcgcgtgt taatcgtccc
    31681 cggctccggt ttggttaaag aacaagccga aaaagaaggc ttggacaaaa ttttcatcga
    31741 agccggtttt gaatggcgcg aaccgggctg ttcgatgtgc cttgcgatga atgccgaccg
    31801 cctcgcgccg aggcaacgct gcgcctccac gtccaaccgc aattttgaag gccgccaagg
    31861 caacggcggg cgcacccacc tcgtcagccc cgctatggcc gccgccgccg ccgttaccgg
    31921 acactttacc gacatccgca cgatggcgta agtattttca ggcagtcttt aagaatttga
    31981 cgaggccgtc tgaaatatgt cccgtaaggc agaaacacct aatattcaaa ccaaggagtt
    32041 taaccatgaa caaacttttc gttaccgccc tgtccgccct cgccttgtcc gcctgcgccg
    32101 gcacttggca gggcgcgaaa caagacaccg cccgcaacct tgacaaaaca caggccgccg
    32161 ccgaacgcgc cgccgaacaa acaggcaacg ccgtcgaaaa aggttgggac aaaaccaaag
    32221 aagccgtcaa aaaaggcggc aatgccgtcg gacgcggcat ttcccatctc ggcaaaaaaa
    32281 tcgaaaacgc caccgaataa ccgatttgcg atgccgtctg aaaccgtttc agacggcatc
    32341 gtcagcacaa gacgcaaacc catgaaagcc tttaccaaaa tcaccgccat cgtcgccccg
    32401 ctcgaccgca gcaacgtcga taccgatgcc atcatcccca aacaatttct gaaatccatc
    32461 aaacgcagcg gcttcggccc caacgccttt gacgaatggc gttacctcga ccacggcgaa
    32521 ccgggcatgg acaacggcaa acgcccgttg aaccccgatt tttccctgaa ccagccgcgt
    32581 taccaaggcg cgcaaatcct gttgacgcgt aaaaacttcg gttgcggctc ttcacgcgaa
    32641 catgcccctt gggcattgga cgactacggc ttccgcgcca ttatcgcccc cagcttcgcc
    32701 gacatcttct ttaacaactg ctacaaaaac ggccttttgc ccatcgtgtt gaccgaagaa
    32761 caagtcgacc ggcttttcaa agaagtcgaa gccaacgaag gctatcggct ctccatcgac
    32821 cttgccgagc aaaccctgac caccccgggc ggcgaaacat tcacattcga cattaccgaa
    32881 caccgcaaac actgcctctt aaacggcttg gacgaaatcg gactgaccct gcaacacgcc
    32941 gacaaaatta aagcctttga agaaaaacgc cgccaaagcc agccttggct gtttaacggt
    33001 taaaacctat gccgtctgaa ggctgttcag acggcattcc tgataacata taattgctgc
    33061 ggctaatgta aacagagggg cgataaattg ttttcatctt ttaagcgggc ttagcaacac
    33121 tctaaattaa tgcaactctc catttcaaat aaatttttag atttttaata tgcaacaaat
    33181 taaatttatt gacttatttt ccggaatgag tggcatcagg aaaggattcg aacaagcctg
    33241 tcggaaacag tcggttgctt gcgaatgtgt ttttacttca gaaatcaaac cggcagcttt
    33301 ggaagtattg aagcaaaact accctgatga agtgccgtat ggagatataa cgaaaattga
    33361 aacaggggat attcccgatt ttgacatcct gttggcagga ttcccttgtc aggctttttc
    33421 tttcgccgga aaaagattag gctttgaaga tacacgggga acgcttttct ttgatgtggc
    33481 aaggatttta aaggcaaaaa aaccaaaagg ttttattttg gaaaatgtgg aaggattggt
    33541 gacacacgat agaaaagatt cgacacaaaa aatagggcgc accctgaccg ttattttgga
    33601 aaccttggaa gcattgggct actatgtttc ttggaaagtt ttaaatgcaa aagatttcgg
    33661 cattccccaa aacaggaagc gcatttatct gacaggcagt ttgaaatcca aacccgactt
    33721 atctttcgaa acaagcccaa gtccgaaatt aaaaaatatt ttggaatcgg gactgcctac
    33781 tgaaagcagc cctttcatca aaaaattgct aaaaaaattt cccccgtccg aactgtacgg
    33841 aaaatcagta aaagacaaac ggggagggaa aaacaatatt cacagttggg atattgaatt
    33901 aaaaggcgca gtaaccgaag aagagaagca attgttaaat atccttctaa aagaacggag
    33961 gaaaaaaaaa tgggcttcag aaatcggcat agattggatg gatgggatgc ctttgacaaa
    34021 agcgcaaatt tcaactttct ataaacaccc cgatctccaa aatattttgg acagcctgac
    34081 agacaaaggc tatttggttt tagagcatcc gaaacaaaaa attggcgggc aaagaatcaa
    34141 agatgaatcc ctgcccaaag gctacaatat tgtttcaggt aaaaaatctt ttgaaatcaa
    34201 taaaatatta gatccaaacg atgttgcgcc aaccttggtt gcaatggata tggagcacct
    34261 tttcgtcgtt gacaacggcg gtttgcgtac acttaccgga aaagaagggt tacgcttatt
    34321 cggctatccg gacgattatt cgtttgatat tcccaaaaaa gacagatgcg atttattggg
    34381 taataccgtt gccgtccctg tgattaaggc ggtatctgaa agacttctgc atactttata
    34441 aaccgacagg gataaaatga taaaacttac tgcacaacaa atatttgata agttgctgga
    34501 tgaagaaaaa atcttatcgg ccaatggtca aattagattt ttcttaggag atgtggatat
    34561 tatcgtcaaa caaaaagatg ttgtcggcaa catcattcag gaatggctcg gcggatggtt
    34621 gagaaaaagg gaaattgaat ttgatgtttc aaccaatacc caaatgcctc ccgatttctt
    34681 tttaaataaa aaagaccgca gcagagaatt gttggaagta aaggcgttca accgaaatgc
    34741 ctgcccgggt tttgatattg cagattttaa aatgtattct gatgaaatca ttcataagcc
    34801 ctatatgctg gatgtagatt atttaatatt cggttacgat atggacgaca acggcaatgt
    34861 aaccatcaag gatttatggc ttaaaaaagt atggcaaatt accagaagca tggatgggtg
    34921 ggcaattaat cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg gtgtttggta
    34981 cagcataaat aaaaagaata tgcctatgtt tgaatgcttg gaagattttg tttccgcaat
    35041 tgaagaaacc gtttatcaaa acccggcaac acggcataac gcatccttat ggaaaaagaa
    35101 atttgaggaa gcttataaaa aacactacaa ccgatcgatt tccatacccc gttggcacga
    35161 aattgcacac aaatacaaaa agaaataacc cttcaaccat caacccataa ggaaacttga
    35221 aatgaccaaa catatcgcca tcctccgagg cgacggcatc ggtcccgaaa tcgtcgccga
    35281 aaccgtccgc gtactcgaca aatttatcgc ccaaggcttg gatgccgact acgaatacgc
    35341 gcctttgggc ggcgaagcct acgacgaata cggccatcct tatccagaat ttacgcaaaa
    35401 cctctgccgc aaagccgatg ccgtcctgct tggtgcagtc ggttcgcccc aatacgacaa
    35461 tctcgaccgt ccgttgcgcc ccgagcgcgg cttgttagca attcgtaagg atttgaacct
    35521 gtttgccaat ttgcgtcctg ccgttttata tcccgaatta gccaacgcct ccacgctcaa
    35581 gcctgaaatc gttgccggtt tggatatttt gattgtgcgc gaacttaccg gcgatattta
    35641 tttcggcgaa ccgcgcggca tccgtgtttt ggaaaacggc gaacacgaag gctacaacac
    35701 catgaaatac agcgaaagcg aaatccgccg catcgcccac gtggccttcc aatccgccca
    35761 aaaacgcagc aaaaaagtct gctccgtagg caaagccaac gttttggaaa ccaccgaact
    35821 gtggcgcgaa atctttgaag aaatcggcaa acaatatccc gatgtcgagc tttcccatat
    35881 gtatgtggac aatgccgcca tgcaactcgt gcgcgcgccc aaacagtttg acgtgattgc
    35941 caccggcaac atcttcggcg acatcctctc cgacgaagcc tccatgctga ccggctccat
    36001 cggtatgctg ccttccgctt ctttggacga aaacggcaaa ggcctgtacg aaccgtctca
    36061 cggctccgcc cccgacatcg ccggacaaaa caaagccaac ccgctggcca ccatcctctc
    36121 gcttgccatg ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac aagtcgaaaa
    36181 cgccgtccaa aaagtgctgc aacaaggctt gcgtaccggc gatatttacg aagaaggcac
    36241 aaaactcgtt tcctgctccg aaatgggcga cgcggtactc gccgccttg
//
LOCUS       DAWTCI010000011        35653 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWTCI010000011 DAWTCI010000000
VERSION     DAWTCI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35653
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.011"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(244..861)
                     /gene="thiE"
                     /locus_tag="WM481_000581"
     CDS             complement(244..861)
                     /gene="thiE"
                     /locus_tag="WM481_000581"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFA9079961.1"
                     /translation="MTFPPLKSLLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGNE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPGYIASGAIFQTTTKQMPTAPQGLDKLREYVEQARGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            complement(883..1983)
                     /locus_tag="WM481_000582"
     CDS             complement(883..1983)
                     /locus_tag="WM481_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9079962.1"
                     /translation="MTRIAVLGGGLSGRLTALQLAEQGYQIELFDKGTRQGEHAAAYV
                     AAAMLAPAAEAVEATPEVIRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADEIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPQDLQAQYDWVIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIAAGLRPTLNHHNPEIRYSRERRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIGRQD"
     gene            complement(1980..3206)
                     /gene="cytX"
                     /locus_tag="WM481_000583"
     CDS             complement(1980..3206)
                     /gene="cytX"
                     /locus_tag="WM481_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFA9079963.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKCGSVLFSVANMLQLAGWTAVM
                     IYVGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATL
                     TATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGITGILAVVLSTVTTTFLDT
                     YSAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVS
                     VRLFFKKTQSLQRNPS"
     regulatory      complement(3272..3376)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3451..4935
                     /gene="tldD"
                     /locus_tag="WM481_000584"
     CDS             3451..4935
                     /gene="tldD"
                     /locus_tag="WM481_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFA9079964.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLCPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQIDQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGGA
                     GGGGRYDLAYFDETLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRQSYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFGASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     gene            5079..5738
                     /locus_tag="WM481_000585"
     CDS             5079..5738
                     /locus_tag="WM481_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9079965.1"
                     /translation="MNWPYLIDAVPKFADAAKLTLELSVYGVVLSLLFDLPVAVVTAY
                     RIRPFYALARAYIELSRNTPLLIQLFFLYYGLPKMGIKWDGFTCGVIALVFLGASYMA
                     EAVRAGILAVPKGQVGAGKAIGLSRFQVFRYVELPQVWAVAVPAIGANILFLMKETSV
                     VSTVGIAELLFVTKDVIGMDYKTNEALFLLFAAYLIILLPVSLLARRIENRVRSAKYG
                     V"
     gene            5728..6396
                     /locus_tag="WM481_000586"
     CDS             5728..6396
                     /locus_tag="WM481_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9079966.1"
                     /translation="MAFEWLFEGKNAARLGEGLLLTAQISLISVAASCVLGTLFGLVL
                     RSRNRLVRFVGRFYLETIRIVPILVWLFGLYFGLSVWTGIHIGGFWVCVWVFSLWGVA
                     EMGDLVRGALESIEKHQVESGLAPGLSRGQVFRCIELPQSIRRVLPGAVNLFTRMIKT
                     SSLAWLIGVIEVVKVGQQIIENSLLTQPNASFWVYGLIFMLYFFCCWPLSLLAAKLEQ
                     KWEH"
     gene            6398..7129
                     /locus_tag="WM481_000587"
     CDS             6398..7129
                     /locus_tag="WM481_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFA9079967.1"
                     /translation="MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKST
                     LLRCVNGLEPHQGGSIVMDGVGEFGKDVSWQTARQKVGMVFQSYELFAHMTVIENILL
                     GPVKVQNRDRAEAEAQAGKLLERVGLLDRKNAYPRELSGGQKQRIAIVRALCLNPEVI
                     LLDEITAALDPEMVREVLEVVLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVE
                     SSDPETFFSAPKSERARQFLAGMDY"
     gene            7242..8096
                     /locus_tag="WM481_000588"
     CDS             7242..8096
                     /locus_tag="WM481_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9079968.1"
                     /translation="MKLNAKLKALLASAAIAVGLTACGGGSGDAQSSQSSGAATVAAI
                     KEKGVIRIGVFGDKPPFGYVDANGKNQGFDVEIAKDLAKDLLGSPDKVEFVLTEAANR
                     VEYVRSGKVDLILANFTQTPERAEAVDFADPYMKVALGVVSPKNKPITDMAQLKDQTL
                     LVNKGTTADAFFTKSHPEVKLLKFDQNTETFDALKDGRGVALAHDNALLWAWAKENPN
                     FEVAIGNLGPAEFIAPAVQKGNADLLNWVNGEIAAMKKDGRLKAAYEKTLLPVYGEKV
                     KPEALLAE"
     gene            8513..9334
                     /gene="prmC"
                     /locus_tag="WM481_000589"
     CDS             8513..9334
                     /gene="prmC"
                     /locus_tag="WM481_000589"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9079969.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEI
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSERQWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVEILPDLAGLDRVTLGKYMKHLK"
     gene            9420..10808
                     /locus_tag="WM481_000590"
     CDS             9420..10808
                     /locus_tag="WM481_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFA9079970.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNVIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLVAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCTGL
                     GFSPLATAAIVGTAGALGDAGSPASDSTLGPTMGLNADGRHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            10809..11033
                     /locus_tag="WM481_000591"
     CDS             10809..11033
                     /locus_tag="WM481_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFA9079971.1"
                     /translation="MDAVQELERRIVELEIQTALQEDVISGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(11433..12326)
                     /locus_tag="WM481_000592"
     CDS             complement(11433..12326)
                     /locus_tag="WM481_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HFA9079972.1"
                     /translation="MPHPCQTVHTLTTHVPKCRLKLNAARRRRYNRPQLFFPNIIMTA
                     TEHDNDDALLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAETLAGRLKRINHTVDVRAVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAAYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVLSPLVGIVGCTQAAEALKILLEAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAAR"
     gene            12369..15071
                     /gene="ppc"
                     /locus_tag="WM481_000593"
     CDS             12369..15071
                     /gene="ppc"
                     /locus_tag="WM481_000593"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFA9079973.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDERQTYDLTLACGLFAQILNIAEDVHHERRRQIHEDAGHNA
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLNFNRRIRALL
                     PQRERCTNADALARLRREIDTVLLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVRVPNILKIGGWIGGDRDGNPFVSGETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARSLGLGMGCKFGFLEPYASAQKFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELGHQRPLYSP
                     FITYSDHTRRELAIFNEARKIKDEFGEDAVTQSIISNCEQPGDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLHQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGSPETLAALRGHAQNNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPEKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            15194..16183
                     /locus_tag="WM481_000594"
     CDS             15194..16183
                     /locus_tag="WM481_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFA9079974.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLAEALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGSVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            16237..16566
                     /locus_tag="WM481_000595"
     CDS             16237..16566
                     /locus_tag="WM481_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079975.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELERAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENARYRALLEQSREKISALAARLP
                     QRQETQQ"
     gene            16577..16882
                     /locus_tag="WM481_000596"
     CDS             16577..16882
                     /locus_tag="WM481_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFA9079976.1"
                     /translation="MNIEQVYIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKITDMDNACQKALSRLGQE"
     gene            16888..17067
                     /gene="ssrS"
                     /locus_tag="WM481_000597"
     ncRNA           16888..17067
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WM481_000597"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            17245..17676
                     /gene="rplM"
                     /locus_tag="WM481_000598"
     CDS             17245..17676
                     /gene="rplM"
                     /locus_tag="WM481_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFA9079977.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVATEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQDQFPDRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            17689..18081
                     /gene="rpsI"
                     /locus_tag="WM481_000599"
     CDS             17689..18081
                     /gene="rpsI"
                     /locus_tag="WM481_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFA9079978.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLTKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESLDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(18462..18956)
                     /locus_tag="WM481_000600"
     CDS             complement(18462..18956)
                     /locus_tag="WM481_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079979.1"
                     /translation="MRLTKYPYNSIRELEDYLINTYQKYIILQEGGKEIYRCFILSFY
                     KEFNIGIGLAVSCISIPPKVLMLDDKNIFIGFDSVVFCISIQNSKVNILNIDGIVFDI
                     YLLDNQKICIIHELGAIITDKNLIRENSVSTDIISDWEIDKVNKLIILKGLDSEKIIS
                     LNYD"
     gene            complement(19149..20078)
                     /locus_tag="WM481_000601"
     CDS             complement(19149..20078)
                     /locus_tag="WM481_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9079980.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQSSIS
                     FHPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            20207..20956
                     /locus_tag="WM481_000602"
     CDS             20207..20956
                     /locus_tag="WM481_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFA9079981.1"
                     /translation="MVLRRDFLAWCDETLQTASFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     VHPILGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVAI
                     GNPEREIRRVAWCTGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            21079..21660
                     /gene="petA"
                     /locus_tag="WM481_000603"
     CDS             21079..21660
                     /gene="petA"
                     /locus_tag="WM481_000603"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFA9079982.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            21679..23028
                     /locus_tag="WM481_000604"
     CDS             21679..23028
                     /locus_tag="WM481_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFA9079983.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            23043..23831
                     /locus_tag="WM481_000605"
     CDS             23043..23831
                     /locus_tag="WM481_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFA9079984.1"
                     /translation="MKQAMKNWFAALLSAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            24163..25953
                     /gene="rmuC"
                     /locus_tag="WM481_000606"
     CDS             24163..25953
                     /gene="rmuC"
                     /locus_tag="WM481_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFA9079985.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAQLAEKAA
                     RCDFIEQAHAEIASELAALDGKYEHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIDLQEKAQGLAVENERLATQLGQERKAFAEQYALERQIRQRVETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAYLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVRQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADEAARELAAYIASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERANEQTAFALGKPSEQEAADEAE"
     gene            complement(26020..26652)
                     /locus_tag="WM481_000607"
     CDS             complement(26020..26652)
                     /locus_tag="WM481_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFA9079986.1"
                     /translation="MTLRYEILPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLETHKVPVLGPHREDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFAFPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            26745..27569
                     /locus_tag="WM481_000608"
     CDS             26745..27569
                     /locus_tag="WM481_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFA9079987.1"
                     /translation="MIKKIIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRRHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNLPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            27638..28201
                     /locus_tag="WM481_000609"
     CDS             27638..28201
                     /locus_tag="WM481_000609"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFA9079988.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVAYEITRDLGD
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            28284..28721
                     /locus_tag="WM481_000610"
     CDS             28284..28721
                     /locus_tag="WM481_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFA9079989.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEGQD
                     DIINNAISALQEFPENHGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMIKAV
                     QYSPAAEDFAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            28942..29211
                     /locus_tag="WM481_000611"
     CDS             28942..29211
                     /locus_tag="WM481_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFA9079990.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFKSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGLDEAAAMKALTDLINDYFGEGE"
     gene            29211..30986
                     /gene="ptsP"
                     /locus_tag="WM481_000612"
     CDS             29211..30986
                     /gene="ptsP"
                     /locus_tag="WM481_000612"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFA9079991.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAQADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIISPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGEVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNAGQLEGDIAKVIRCEDE
                     EKSEKLIKQINSVSVAEEAEVKGRK"
     gene            complement(31113..32054)
                     /locus_tag="WM481_000613"
     CDS             complement(31113..32054)
                     /locus_tag="WM481_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9079992.1"
                     /translation="MLELNGLCKCFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIRLNGENITCMPPEKRRISLMFQDYALFPHMSALENTAFGLKMQK
                     MPKAEAERLALSALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LIQTPAGVQVARLMGLPNTDDDRHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHP
                     EHGELTLNLTVGQHTDGISGNGTVRIRVDEGRIVRFR"
     gene            complement(32063..32938)
                     /locus_tag="WM481_000614"
     CDS             complement(32063..32938)
                     /locus_tag="WM481_000614"
                     /inference="COORDINATES: protein motif:HMM:NF012514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9079993.1"
                     /translation="MPTVRFTESVSKQDLDALFERAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERIKKDWEAGCSESSDGIFLNADGWPDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLVESNGRWHFWIGRRSPHKAVDPGK
                     LDNIAGGGVSGGEMPSEAVCRESSEEAGLDKTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVAGFEKMDIGGLLDAMLSKNMMHDAQLVTLDAFYRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(33121..33197)
                     /locus_tag="WM481_000615"
     tRNA            complement(33121..33197)
                     /locus_tag="WM481_000615"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33161..33163),aa:Met,seq:cat)
     regulatory      33346..33445
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33536..35437
                     /gene="thiC"
                     /locus_tag="WM481_000616"
     CDS             33536..35437
                     /gene="thiC"
                     /locus_tag="WM481_000616"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFA9079994.1"
                     /translation="MTTPKKTAKISGNEARELSDLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGAYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIECAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFAAIRERMKLDELFRRPEYAKLLKQHTGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQTLEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         8427 a         9650 c         9826 g         7750 t
ORIGIN      
        1 ctagccgaat tactttattt tttgatacgc aaccggccgg ttgccgtcat tcccgcgcag
       61 gcgggaatcc atttttgaaa ttcggaaact gtttttcaaa tgatggtttc tcaagtttta
      121 cggcggattc ccgcctgagc gggaatgacg gaatttgatg atatgccaca tttaaagtta
      181 agcatatttg cgataacggg ctaagttttg cctacacggc aattgaattt tcttctttcg
      241 gttttatcca tcccacaaag cctgaaacgc tttaaccacc gcttcgggat ttgccgcttc
      301 ggttacggcg cggacggcgg cgagtgagga aactccggtg gccagcacgg ctcgggcgtt
      361 gttcaaatcg atgccgccga tggcgacgac gggcgtgccg cgagcctgtt ccacatattc
      421 gcgcaatttg tccaagcctt gcggggcggt gggcatttgt ttggtcgtgg tctggaaaat
      481 cgcgccgctg gcgatgtagc cggggtgtac ggacagggcg cggtcgagtt cggcgacgga
      541 gtgcgtactc aaacccaaac gcaaaccggc ggcggcgatg gcggcaaggt cggcggtgtc
      601 catgtcttcc tgcccgagat gtacgccgta tgcgcccgct tcgattgcct cgcgccagtg
      661 gtcgttgatg aaaagctgtg tgcggctgcc ttgacaggcg gcgacgcagc gggcgatttc
      721 gcgtttcaat tcgttgccgt gcaaggtttt acagcgcagt tgcaccgtgt cggcacctgc
      781 tttgaccatg cgccccaccc aatcggcggt gggaacgacg gcgtagaatt taagtaggga
      841 ttttaggggc gggaaggtca tagggtgtcc tttcgggcgg ctttaatctt gtcttccgat
      901 atacgccaaa ccgctttctt catcacgttc gggcgcgtct tttccgtcaa acagtgccac
      961 tgccaatctg acggcggcgg cggttacggc gggggaaatc ataaagccgt gccggaaaag
     1021 gccgttgatt tcgatgaggc ggcgttcgcg gctgtagcgg atttcggggt tgtggtggtt
     1081 gagcgtgggg cgcaggccgg cggcgatttc gaggatgtcg gcttcgccga aggcggggtg
     1141 gacggcatag agcgcggata agagttccag cccggaacgt acgctggcgg gggcttggct
     1201 ttcgctttcg atttgggtcg cgccgatgac gaagacgtgg ttttctttcg gggcgatgta
     1261 gagcggatag cgcgggtgca gcaggcgcac ggggcggttg agcgtgattt cgggcgtgta
     1321 aacccgcgcc acttcgccgc gtatgccgcg caaggtgctg gtgtgctcgg gggattggtt
     1381 ccacgcggtt ttcgcgccgt agccccggca gtcgattacc cagtcgtatt gggcttgcag
     1441 gtcttggggg gcgcattcgt gttcccaatg gcaagggacg ttcagttcgt ccaaagcgtc
     1501 ggcaagtgca gacaatattt gccgcccgtc gagctggcct tcggtcggca ggtagatgcc
     1561 gtctgaaaaa cgtccgccga gttgcggttc gcgttcggcg atttcatcgg cgcgccaacg
     1621 gacgatttcg tcatccgcta cgccgccgcg tttgagatgg cggacgaact cgctggataa
     1681 tggcttgtcc tgcccgtgcc acacaatcag gctgccgttt tcctgcatca tcgtgtgcgt
     1741 gttcagacgg catcggatgc cgcgccaaag cggaatgctc tgcctgccca gcctgatgac
     1801 ttcgggcgtt gcctcgaccg cttccgccgc aggcgcgagc atcgcggcgg caacataggc
     1861 ggcggcgtgt tcgccttggc gggtgccctt gtcgaaaagt tcaatctgat aaccttgttc
     1921 tgcaagctgc aatgcggtca gccttccgga aaggccgcct ccgaggacgg cgatacgggt
     1981 catgacgggt tcctttgtaa agattgggtt tttttaaaga aaaggcgtac cgatacggtg
     2041 gcaatggcaa cggcagacat tacgggggcg gtcagaccga tgctgctttc ccaaccggac
     2101 gagagcagga agcggtagag gatgaagcct gccagccaca gaaccagtcc ggcaaagtca
     2161 aagccttcaa tctcctcacg ccgttttaag acgaaaaagt cggcaatcaa aaccgccgcc
     2221 atcggcgcaa ataccgagcc gataagcagc aggaagtttt catattcggt aacgggcagc
     2281 atgacggcaa gcaccgtgcc gatcagggta acgccgacag cgacgggtgt ttccgcaaaa
     2341 cgcgcggaaa tgttgttcgc actcgcgccg gcggaatagg tatcgagaaa cgttgtggta
     2401 acggtggaga ggacgactgc cagaatgccc gttatgccca agcccgcgcc caacaggatt
     2461 ttcgccacgt cggtttctcc ggtaaacaga gccgccgcca aacccaaggc atacatccag
     2521 cagcccgtca gcgtataggc gagcgttgcc gtcagggttg ccgcaaacgg gcggcgtgct
     2581 tggcgcgtgt agtcggcggc cagcggcagc caggaaagcg gcatgacggc ggacagttcg
     2641 actgccgttc cgaaggtcat gccgtctgaa acggcgggcg cggcgtttgt gccggacgaa
     2701 gcgaacactt cgacgctcaa ccacaacacg gcaagcagca tcagcagcat cgaaacggtt
     2761 ttcagcccgc ccgttctgcg tgcgccgaaa accagccaca gcacgatcag tgcgccgttt
     2821 gccaatgccc accagacaaa ggattcgccg tcccacaaca ctttgcccaa agcggagctg
     2881 accgttgcgc cgacgtaaat catcaccgcc gtccagccgg ccagttgcag catattcgcc
     2941 acggaaaaca gcactgaacc gcatttgccg aacgacaggc gcacactttc catcgagctg
     3001 cgtccggtca gtgcgccgat atacgctgcc gcaaaaaaca gcgcgccgcc gacggcatga
     3061 cccaaaagca gggccgccag accgcgctgc cagcccaagg gggcgagcag cgtacccgtg
     3121 ctgatttcgg caatcgatac cgccgcgccg aaccaaatca gcccgatggc ggcggaagat
     3181 gaaggagagg aggcattgcc cgacatatga cgtcctttaa aaagaaggaa ggtgggcggg
     3241 gaagtgcgga aaagggaacg gaagcccgaa agggcgggga ggttttgttt cctacgcagg
     3301 cattaccccg acaggttcta cggattttgc tttcgcaatc tcagccgccc ggcgggcggc
     3361 actccgacaa gaaggcttta agtttacaca aagcaacgtg cgttggcaaa attcttacat
     3421 tttcgacggc acggcgcatt tccataacaa atgccgtata atcaagcccc tgatttttta
     3481 tgcgccgaca ctatgcatcc gacctattcc gccgtacagg cgcggctgct cgaagccaac
     3541 cgcctttgcc ccgaactgct cgccaaaagc ctgtgcatca tcggcgcgca ccacgtcgat
     3601 tatgccgata tctactgcca gcgcaccgct tatgaaagct ggcatttgga agagggcatc
     3661 gtcaaatcgg gcagcttcca aatcgatcag ggtgtgggcg tgcgcgccgt ttcgggcgac
     3721 aaaaccgcct ttgcctacgc cgacagcctg tgcatcgatt cgataaaccg ttccgcccgc
     3781 gccgtccgcg cgattggggc ggcaggcggc aaggtgtccg ccaaaatgcc gtctgaaacg
     3841 cgcggcaagc cggtttgttc cgcatccgac cccattgccg gcctcgattc cgccgccaaa
     3901 gtcgcgctgt tgaacaaagt ggaagcaatc gccaaagccg ccgatccgcg catcgtgcaa
     3961 gtgatggccg gtttgacctg cgaatacgat atggtttacc tcgcccgtct ggacggcaaa
     4021 cacgccgccg acatccgccc gatggtgcgc ctgaacgtta ccgtcatcgc caaacagggc
     4081 gaacgccgcg aacagggcgg cgcgggcggc ggaggacgct acgacttggc ttatttcgat
     4141 gaaaccttgg tgcggcagtt tgtcgatgcc gccgtcaaac aagccctgac caatctcgaa
     4201 tcccgtcctg cgcctgccgg cgagatgacc gtcgttttgg gcaacggttg gccgggcgtg
     4261 ttgctgcacg aagcggtcgg acacggtttg gaaggcgatt tcaaccgcaa ggggaccagc
     4321 gtcttttccg gccggatcgg cgaacgcgtc gccgccaaag gcgttaccgt cgtcgatcag
     4381 ggcgatattg ccggccggcg cggttcgctc aacatcgacg acgaaggcaa cgaaacacgc
     4441 cgcaccgtat tgattgaaga cggcatcttg gtcggctata tgcaggacga aaccaacgcc
     4501 cgcctgacgg gtacgcaatc gaccggcaac ggccgccgcc aaagctacgc ctccgtcccg
     4561 atgccgcgta tgaccaacac ctttatggaa aacggcagct acgagccgga agaaatcatc
     4621 gcgtccatcg acaagggcat ttacgccgtc aacttcggcg gcggacaggt ggacatcacc
     4681 agcgggaaat ttgtcttcgg cgcgtccgaa gcgtggtggg tggaaggcgg caggctgcaa
     4741 taccccgtca aaggcgcgac cattatcggc aacggccccg aagtgctgaa acacgtttcg
     4801 atgataggca acgataccgc gctcgacagc ggtgtcggcg tgtgcggcaa agaagggcag
     4861 agcgtccccg tcggcgtggg gcagccgacc ctgcgtatcg atgccgggct gaccgtcggc
     4921 ggcagcgcaa tctgacgcgg aatccggcac gatgccgtct gaaaggtttt cagacggcat
     4981 cgccttattc ttttttccga tgtttgatat tcctaagcat tctttaacat tatcttacaa
     5041 gttcattaaa ataccgcctt tcgttaagct gatttctgat gaactggcct tatttaatcg
     5101 atgccgtacc caaattcgcc gatgcggcaa agctgacgct ggaattgtcc gtttacggcg
     5161 ttgttttgtc gttgctgttc gacctgcccg tcgccgtggt aacggcatac cgcatccgcc
     5221 ccttctacgc attggcgcgc gcctatatcg agctgtcgcg caatacgccc ctgctgatcc
     5281 aattgttttt cctatattac ggtctgccga aaatgggcat caaatgggac ggtttcacct
     5341 gcggcgtaat cgcgctggtt ttcttgggcg caagctatat ggcggaagct gtccgcgccg
     5401 gcatcctcgc cgtccccaaa gggcaggtcg gagcgggcaa ggcaatcggt ttgagccgct
     5461 ttcaagtgtt ccgctatgtc gaattgccgc aggtttgggc ggtcgccgtt cccgccatcg
     5521 gcgcaaacat attgttttta atgaaagaaa catccgtcgt cagcacggtc ggcattgcgg
     5581 aattgttatt tgttaccaaa gacgtcatcg gtatggacta caaaaccaat gaagccttgt
     5641 tcctgctgtt tgccgcttat ttgattatcc tgctgcctgt ttctttgttg gcgcggcgga
     5701 ttgaaaaccg cgtacggagt gcgaaatatg gcgtttgaat ggctgtttga aggaaaaaat
     5761 gcggcacggt tgggcgaagg cctgctctta acggcacaaa tttctttaat ctctgttgcg
     5821 gcttcttgcg tattgggcac gctgttcggc ttggttttgc gttcgcgcaa ccggcttgtc
     5881 cgctttgtcg gacggtttta tctcgaaacc atccgaatcg tgccgatttt ggtgtggctg
     5941 ttcggcctgt atttcggcct atccgtctgg acaggcatcc atatcggcgg attttgggtc
     6001 tgcgtctggg tattttccct gtggggcgtt gccgaaatgg gcgacttggt gcgcggcgca
     6061 ctggaatcga ttgaaaaaca ccaggtcgaa tcgggtcttg cgcccggctt gagccggggg
     6121 caggtattcc gctgcatcga gctgccgcaa agcatccgcc gcgtattgcc cggcgcggtc
     6181 aacctgttca cgcgcatgat caaaaccagc tcgctcgcct ggcttatcgg cgtgatcgaa
     6241 gtcgtcaaag tcgggcagca aattattgaa aactcgttgc tgacgcagcc caatgcttca
     6301 ttttgggttt acggcctgat ttttatgctg tatttcttct gttgctggcc gctatctcta
     6361 ctggcggcaa aacttgaaca aaaatgggaa cactgacatg gctttactga gcatccgcaa
     6421 gctgcacaaa caatacggca gcgtaaccgc catccaatcc ttagacttgg acttggaaaa
     6481 aggcgaagtc atcgtactgc tgggcccgtc cggctgcggc aaatccaccc tcctgcgctg
     6541 cgtcaacggt ttggagccgc accaaggcgg cagcatcgtg atggacggtg tcggcgaatt
     6601 cggcaaagac gtttcctggc aaaccgcccg gcaaaaagtc ggtatggtct ttcaaagcta
     6661 cgaactgttt gcccacatga ccgtcatcga aaacatcctc ttaggcccgg taaaggtaca
     6721 aaaccgcgac cgtgccgaag cagaggcgca agccggcaaa ctgttggaac gcgtcggact
     6781 gctagaccgc aaaaacgcct atccgcgcga actttccggc ggtcagaaac agcgcatcgc
     6841 cattgtccgc gccctgtgcc tgaatccgga agtcatcctg ctggacgaaa tcaccgccgc
     6901 acttgacccc gaaatggtgc gcgaagtctt ggaagtggtt ttggaactcg cccgcgaagg
     6961 gatgagtatg ctcatcgtaa cccacgaaat ggggttcgca cgcaaagttg ccgaccgcat
     7021 cgtctttatg gacaaaggcg gcatcgtcga atcgtccgac cccgaaacct ttttttccgc
     7081 accaaaaagc gaacgcgccc gccaatttct ggcaggtatg gactactgat ttaccgcaaa
     7141 ccgtccgaac ccttttcaga cggcatttca tagggcagtg ctttatgtgt cgtcctgtgt
     7201 gttgaaacat cccaagcaaa cattcaaaaa ggagatttcc aatgaaattg aacgccaaac
     7261 tcaaagccct tttggcttcc gccgccatcg ccgtcggtct gaccgcctgc gggggcggct
     7321 ccggcgatgc ccaatcttca caaagcagcg gtgcggcaac cgttgccgcc atcaaagaaa
     7381 aaggcgttat ccgcatcggc gtattcggcg acaaaccccc gttcggctat gttgacgcaa
     7441 acggcaaaaa ccaaggcttt gacgttgaaa tcgccaaaga cctggccaaa gacctgctcg
     7501 gcagccccga caaagtcgaa ttcgtcctga ccgaggccgc aaaccgcgtc gaatacgtcc
     7561 gttccggcaa agtcgacctc atcctcgcca actttaccca aacccccgaa cgcgccgaag
     7621 ccgtcgattt cgccgatcct tacatgaaag tggcgttggg cgtggtttcc cccaaaaaca
     7681 aaccgattac cgacatggcg caattgaaag accaaaccct gctggtcaac aaaggcacca
     7741 ccgccgacgc tttcttcacc aaaagccatc ccgaagtcaa gctgttgaaa ttcgaccaaa
     7801 acactgaaac cttcgacgcg ctgaaagacg gtcgcggcgt agcactcgcg cacgacaacg
     7861 ccctgctgtg ggcgtgggcg aaagaaaacc cgaactttga agtcgccatc ggcaatctcg
     7921 gtcctgccga atttatcgcc cccgccgttc aaaaaggcaa cgccgacctc ctgaactggg
     7981 tcaacggcga aatcgccgcc atgaaaaaag acggccgtct gaaagccgcc tatgaaaaaa
     8041 ccctcttgcc cgtgtatggc gagaaagtca aaccggaagc attgttggcc gaataagttt
     8101 gtttgacaag aaaatccgta tccgcatttg aaggcgggtg cggatttttt ttcagccgcc
     8161 gaaaatattg caaattcaaa ggaaaaatca actatctcat cgctttattt attttattat
     8221 tcttgcgcgt gcggaaatca agttcgtccg gttttgcctg tttttgaatt tcgggaaact
     8281 tccaaatcgt cattccagat aaaacgcttc agccttatgt ttccggatga ccgttttcgc
     8341 gggatgacgg cggagcgttt ctgttattcg gataaattcc tacggtattg aaacggcgat
     8401 aaggtgtttt cctttattat ataatttcaa tgcaaaatga tattttttga acaagcgtgc
     8461 cgcaaagccg gaaaaaatgt tcaagccgca tcggcggcag acgggacgaa aaatgacgtt
     8521 tgacgaatgg ttgggcttgt caaaactgcc taaaattgaa gcaaggatgc tgctgcaata
     8581 tgtttcggaa tatacgcgcg tgcagttgtt gacgcggggc ggggaagaaa tgccggacga
     8641 aatccgacag cgggcggaca ggctggcgca acgccgtctg aacggcgagc cggttgccta
     8701 tattttaggt gtgcgcgaat tttatggcag acgctttaca gtcaacccga acgtgctgat
     8761 tccgcgcccc gaaaccgaac acttggtcga agccgtattg gcgcgcctgc ccgaaaacgg
     8821 gcgcgtgtgg gatttgggga cgggcagcgg cgcagttgcc gtaaccgtcg cgctcgaacg
     8881 ccccgatgcg tttgtgcgcg catccgacat cagcacgccc gcccttgaaa cggcgcggaa
     8941 aaatgcggcg gatttgggcg cgcgggtcga atttgcacac ggttcgtggt tcgacaccga
     9001 tatgccgtct gaaaggcaat gggacatcat cgtgtccaac ccgccctata tcgaaaacgg
     9061 cgataaacat ttgtcgcaag gcgatttgcg gtttgagccg caaatcgcgc tgaccgactt
     9121 ttcagacggc ctaagctgca tccgcacctt ggcgcaaggc gcgcccgacc gtttggcgga
     9181 aggcggtttt ttgttgttgg aacacggttt cgatcagggc gcggcggtgc gcggcgtgtt
     9241 ggcggagaat ggtttttcgg gagtggaaat cctgccggat ttggccggtt tggacagggt
     9301 tacgctgggg aagtatatga agcatttgaa ataattgttt gcaaaattgg cggattttcc
     9361 gttattgggt tatgatgtgt tttgatatgt aaacatttat aggtttggag cgataaacaa
     9421 tgaatgcagt cgtagttgcc gtaatcgtta tgctggtgct gtcgctgtcg cgcgtgcacg
     9481 tggtattgag cctgacggtc ggcgcgtttg tcggcggcgc ggtggcgggt atgccgctgc
     9541 aaaacattgc cgatgcggcg ggacaggtca gtcaggcggg gattatcccc gtgttcaaca
     9601 aaggtttgga aggcggcgcg aagattgcgc tttcttatgc gatgcttggt gcgtttgcga
     9661 tggcgattac ccattccggc ctgccgcagc agcttgccgg cgcggtcgtc cgcaagctga
     9721 accggggcgg tatgcccgac agcgtgcgtt cgggcgaggg cgcggtcaaa tggctgctgc
     9781 tttccatcat ccttgtgatg ggcattatga gtcagaacgt catacccatc cacatcgcct
     9841 ttatcccgat gattgttccg ccgctgcttt tggtgtttaa ccgcctgaaa atcgaccgcc
     9901 gcctgattgc gtgcgtcatc actttcgggc tggttacgac ttatatgttc ctgccttacg
     9961 gcttcggcgc gatttttttg aacgaaatcc tgttgggcaa catccattcc gccgcgccgc
    10021 agcttgatgt gaaaaacatc aacgtgatgg cggcaatggc gattcccgca ttgggaatgc
    10081 tggccggact cctgctggcg tttgtccact accgcaaacc gcgactgtac caaagcaaca
    10141 atgccgatac ggcgggcaac gccgatgcgg caaaccgtcc gcagccgtcc gcctaccgca
    10201 gcctggttgc cgccgccgcc atcgccgtat gctttgccat acagttgatg tatgaagatt
    10261 cgctggtgtt gggcgcgatg ctcggtttcg ccgtatttat gatgttgggg gtcattaacc
    10321 gcgacaaggc aaacgacgta ttcggcgaag gcatcaagat gatggcgatg gtcggcttca
    10381 ttatgattgc cgcgcagggt tttgccgccg tgatgaatgc gaccgggcat attcagccgc
    10441 tggtggaaag cagtatggcg atattcggca acagcaaagg tatggcggcg ttggcgatgc
    10501 tggtggtggg gcttttggta acgatgggca tcggttcgtc cttttccact ttgccgatta
    10561 ttgccgcgat ttatgtgccg ctgtgtaccg gtttgggctt ttcgccgctt gccaccgccg
    10621 ccattgtcgg cacggcgggg gcgttgggcg atgccggttc gcccgcgtcc gattccacgc
    10681 tgggcccgac gatggggctg aacgccgacg ggcggcacga ccatatccgc gattccgtca
    10741 tcccgacctt catccactac aacatcccgc ttctgattgc cggctggatt gccgcgatgg
    10801 tgctgtaaat ggacgcggtt caagagttgg aacgccgtat tgtcgaactg gaaatccaaa
    10861 ccgcgcttca ggaggacgta atctccggtc tgaacgcgat ggtggcggaa ttgcggcaga
    10921 cgttggattt gcagcaggct cagttgaggc tgctgtatca aaaaatgcag gacaggaatc
    10981 ccgacgcgca agagccgtat tccctgcgcg acgagattcc gccgcattat tgatgcgccg
    11041 ccgtatccgg atttccttaa ataaaggctg tgtttgaata ttccgccggt gcaaccctaa
    11101 gatgtaacct taatcagtcg gacaaaatgc cttttatccg atgaaaccgg ttcttgtccg
    11161 aacggcgatg agcggcttcc cccgctgcaa acagataaaa ccaaccgggt attcaaacac
    11221 agccaaaaaa agccgtccga acccaaaggg gacagacggc caaacacatt acggggaaaa
    11281 cgtcttactc aatgagtctg cgaaacagac attgctaaaa caactgcaat tataaggtaa
    11341 gtttattttt ttgcaaagaa atgtaaattt ttaaattttt aaaaccgttc cgccgcatcc
    11401 gttcggatgc cgtctgaaac ggcggcaggg ttttaccgtg ccgcgccgca aaccgggcat
    11461 tcggggttgc gcggcaggtc gaaatattgc cagccccctt ccaaggcacg gtaaaccgcc
    11521 agcctgccgt gcgacggttc gcccgcttcc agcaggattt tcagagcctc cgccgcctgt
    11581 gtgcagccga cgatgccgac cagcggcgag agcacgccga agagggaaca gatgccgtct
    11641 gaagccgatc cgccgtcgaa caggcaggcg taacacggcg agtcgggcaa gtcgggacgg
    11701 tatgcggcaa gctgtccttc aaaacgtacc gccgcccctg aaaccagcgg tgttttcgtt
    11761 tgcacgcagg cacggttgac cgcctgccgc gtggcgtagt tgtcgcagca gtctaaaacg
    11821 atgtcggcgg tttgaaccaa accggtcagg cggcagccgt cgagtttttc gttgacggcg
    11881 cggacatcga cagtatggtt gatgcgtttc aggcggcctg ccaaggtttc ggctttaggc
    11941 ttgccgacat cgccctcgtc aaatgcgact tggcgttgca ggttgtgcag ctcgaccgtg
    12001 tcggaatcgg ctatggtcag cgtgccgacg cccgaggcgg cgagataggg cagggcggcg
    12061 gcgcccaatc cgccgcagcc gacgaccaaa atatgcgcgg cggaaagttt ctgctgccct
    12121 tcgatgccga tttcgtccaa gaggatgtgg cggctgtacc gcagcaggag tgcgtcgtcg
    12181 ttgtcgtgtt cggtcgcggt catgatgatg ttcggaaaaa acagttgcgg gcgattgtaa
    12241 cgccgccgtc gggcggcgtt caacttcaga cggcatttcg ggacatgagt ggttaaagtg
    12301 tgaacggttt ggcacggatg cggcatttga ggtacattta cagtatttac ggcaatgaga
    12361 ggaaaatcat gcaactgcat atcctgaaca atccgaagga cgcggctttg gcggcggacg
    12421 cggaattttt gaaacagtcc ctgttcaacc tcctgcacga agaagcctcg ccgttggttg
    12481 tcgaaacggt caaactcttg tccacttccg acgacagcgc ggcattgatt gaaaaagtat
    12541 tgccgcaatt ggacgaacgg cagacctacg atctgacgct tgcctgcggg ctgttcgccc
    12601 agattctgaa tatcgccgaa gacgtgcacc acgaacgccg ccgccaaatc cacgaagatg
    12661 ccggccacaa cgcagcagaa ggcagcctga cggaaaccgt ccgcaggctc aaagcgggga
    12721 aagccgacgg caaatcggtg cagcggcagt tggacaatac gtccgttacc gccgttttga
    12781 ccgcgcaccc gaccgaagtg caacgccaaa ccgttttgaa tttcaaccgc cgcatccgcg
    12841 cgctgctgcc gcaacgcgaa cgctgcacca atgccgacgc gctggcacgg ctgcgccgcg
    12901 aaatcgacac cgtgcttctg ggcttgtggc agaccagcga aacgcgccgc cacaaactca
    12961 gcgtcaacga cgaaatcaac aacggcgtgt ccatcttccc gatgagcttt tttgaagccc
    13021 tgcccaaact ctaccgcaag atggaacacg actttcagac ggcctatccc gatgtccgcg
    13081 ttccgaacat ccttaaaatc ggcggctgga tcggcggcga ccgcgacggc aatccgtttg
    13141 tttctggcga aaccctgcgc tttgccttcc gccgccacgc cgatgccgtg ttccgttttt
    13201 accgcagcga actcgacaaa ctttaccgcg aactgccgct ttccatccgc cgcgtcaaag
    13261 tcaacgacga cgtaatggca ctggcagccc tctcgcctga cgaagaaatc gcccgcaccg
    13321 aagaacctta ccgccgcgcc attgcctaca ttatggcgcg cgctatgggc aaagcgcgct
    13381 cgctcggttt gggcatgggc tgcaaattcg gctttctcga gccttatgct tcggcccaaa
    13441 aatttctcga tgatttgaaa aaattgcaac gttcccttat cgacaacggc agccgcctgc
    13501 ttgccgaagg ccgtttggca gacctcatcc gttccgtgtc cgtgttcggc ttccacatga
    13561 tgccgctcga cctgcgccaa cacgcaggca aacacgccga tgtggttgcc gagcttttcc
    13621 aacacgcagg cttggaagac tacaacagcc tgaacgaaga gcaaaaacaa gccgccttgt
    13681 tgcgcgaatt gggccaccaa cgtccgctgt acagcccgtt catcacatac agcgaccata
    13741 cccgccgcga attggcgatt ttcaacgaag cgcgcaaaat caaagacgaa tttggcgaag
    13801 atgccgtaac acaaagcatt atttccaact gcgaacaacc cggcgacctg ctcgccttgg
    13861 cattgctgct gaaagaaagc ggcctgttgg cggtggaaaa cggcaaaccg cacagccgca
    13921 tcaacatcgt gcctctgttt gaaaccatcg aagcgctcga aaatgcctgt ccggtcatgg
    13981 aaaccatgtt ccgcctcgac tggtatgatg ccctgctcga aagccgcggc aacatccaag
    14041 aaatcatgct cggctattcc gactccaaca aagacggcgg ctacgttacc agctcatggt
    14101 gtctgcatca ggcggaattg ggcttggtcg aactcttcaa aaaatacgat gtccgcatgc
    14161 gcttgttcca cggtcgaggc ggcagcgtcg gacgcggcgg cggcccgtct taccaagcca
    14221 tcttggcgca acccgcaggc agcgttgccg gacaaatccg cattaccgag caaggcgaag
    14281 tcatcaccgc caaatacgcc gaccccggca acgcccaacg caacttggaa accttggttg
    14341 ccgcgacttt ggaagccagc atcctgccgg ataaaaaaga ccctgatgcc aaactgatgc
    14401 aggcattgtc ggacgtatcg ttcaaatact accgcgaact gattacccac cccgacttca
    14461 tcgactactt cctgcaaacc agcccgattc aggaaatcgc caccctcaac ctcggcagcc
    14521 gtcccgccag ccgcaaaacc ctggcgcgaa ttcaggattt acgcgcgatt ccgtgggtat
    14581 tctcctggat gcaaaaccgc ctcatgctgc cggcttggta cggtttcggc agcgcggtgg
    14641 aaaccttgtg cgaaggcagc cccgaaaccc tcgccgccct gcgcggacac gcccaaaaca
    14701 accccttctt ccaagcgatg ctctccaata tggaacaagt gatggcgaaa accgacatca
    14761 ccttggcgga aaactacgcc ggtttgagcg aatcacccga aaaggcaaaa gtcatcttcg
    14821 gcatgattaa ggaagaatac cgacgcagcc gcaaagccct gctcgacctg ctgcaaaccg
    14881 aagaactttt gcgcgacaac cgcagcctcg cccgttcgct cgctttgagg attccatacc
    14941 tgaacgcgct caacggtttg caagtcgcca tgctcaaacg cctgcgcaaa gaacccgaca
    15001 atccgcacgc cctgctgatg gtgcacctga ccatcaacgg cgtggcgcaa ggtttgcgca
    15061 ataccggctg atagtgccgc atcggggcaa aatgccgtct gaacgccttt cagacggcat
    15121 ttacctgacc gcacttgcag agaaacaccg attgttttaa agtgaacggc agtgatatgt
    15181 tgaaagacga ccaatgaaaa ttaccgttat cggcgcaggt tcgtggggta cggcgctcgc
    15241 cctgcatttt tcccaacacg gcaaccgcgt atccctgtgg acgcgcaacg cagaccaagt
    15301 ccgccaaatg caggaagcgc gtgaaaacaa acgcggactg cccggctttt cctttcccga
    15361 aaccttggaa gtgtgtgcgg atttggcaga ggcgctcaaa gacagcggac ttgtccttat
    15421 cgtaacctcc gttgccggat tgagaagcag cgcagagctg ctcaaacagt acggcgcggg
    15481 acacctcccc gtcctcgccg cctgcaaagg cttcgagcag gatacagggc tgctgacctt
    15541 ccaagtcttg aaagaagtat tgcccgacaa taagaaaatc ggcgtacttt ccggtccgag
    15601 ttttgcacag gaactcgcca aacaactgcc ctgcgccgtc gtcctcgcct ccgaaaacca
    15661 agagtggatt gaagaactcg taccgcagct caacacgacc gtcatgaggc tttacggcag
    15721 taccgacgtt atcggcgtgg cagtaggcgg ttcagtcaaa aacgtcatgg caatcgctac
    15781 cggactctcc gacggactcg aatacggact caacgcccgt gccgcactgg ttacgcgcgg
    15841 actggccgaa atcacccgcc ttgcctccgc aatgggcgcg cagcccaaaa ccatgatggg
    15901 gcttgccggc atcggcgatc tcatcttgac ctgtaccggt gcactttcgc gcaaccgccg
    15961 cgtcggcttg ggtttggcag aaggcaagga actgcatcag gtgctggtcg aaatcgggca
    16021 cgtttccgaa ggggtcagca ccatagaaga agtcttcaat accgcctgca aataccaaat
    16081 cgatatgccg attacccaaa ccctgctgca actcatccgc aaagaaatga cccctcagca
    16141 ggtcgtcgaa aggctgatgg aacgcagcgc gcgtttcgaa taaacaacaa acagatgccg
    16201 tctgaacccc cagacggcat acagacaggt aaggtcatga aacaaaatat cgaaaaactc
    16261 gaaagcagcg tttatacgtt ggtacaaaaa ttcgaaaccc tcgtcagcga aaaccgccgc
    16321 ctcaaagaaa ccgtcgccga actcgaacgg gcgcacgagc ggcaaaaact cgaacacgag
    16381 accgccgtcg acgaactcag cgaagccctg ctcgtccaag tcggcaaact caaagaagac
    16441 ctgcaaaaca aaatcgacag cctgacggaa gaaaatgcac gataccgcgc cctgctcgaa
    16501 cagagcaggg agaaaatcag cgcactggca gcgcgcctcc cccaacggca ggaaacgcag
    16561 caataaggat taaaggatga acatcgaaca agtctacatc gaagtcatgc acgcccggct
    16621 gaccgtcaac acgccggcag aagaaaaaga cacactgttg caggcagtcg gaatgctcaa
    16681 cggcaaagcc gaagccatcc gcgaaggcgg ccgcgtcgcg gacagcgaaa aaatcgtcat
    16741 tatggccgcg ctcaacgtcg tccacgacct attgaaaacc tccctgaacg gcggcgattt
    16801 ggcaatcggc gattttgcgc gtaaaataac cgatatggac aacgcctgcc aaaaggcact
    16861 atcccgcttg gggcaggaat aacccctttt tccctgcggt gtccgcgaag gcatatattc
    16921 ctttgaacca ataagttcgc ttaaggttgc cgggagcagt agtgggcgcg agcgtccttt
    16981 tgcggacgca cccgaaacta cccggggcag ccgccttgtc agcaaggttc aagcggattc
    17041 ggccgaaacg gctctcgcgg ggattcccat tcaaaaccgc atcgcaacga tgcggttttt
    17101 cacattggcc gccgccccgc ccgaaaaagc agtatgcagt ctgccgaaat ccggaaaatc
    17161 cttccgtaac gcggtaatgc gattgacaaa atcaaatcct gtctttaaaa tccataactt
    17221 tctatatcta tctggatttc cactatgaaa accttttcag cgaaacccca cgaggtgaag
    17281 cgcgaatggt tcgtcatcga tgcccaagac aaagtcttgg gtcgcgttgc aaccgaagtc
    17341 gccagccgtc tgcgtggcaa acacaaacct gaatacaccc cccacgtcga taccggcgat
    17401 tacatcatcg tcatcaatgc ggacaaactg cgtgtaaccg gtgccaaatt cgaagataaa
    17461 aaatacttcc gccattccgg ttttccaggc ggcatctacg agcgcacttt ccgcgaaatg
    17521 caagatcaat tcccggaccg cgctttggag caggctgtaa aaggtatgct gcccaaaggt
    17581 ccgctgggtt acgccatgat taaaaaactg aaagtgtacg ctggtgcgga gcatgcccat
    17641 gctgcgcaac aacccaaagt tttggaactg aaataataag gacacgacat gaacggtaaa
    17701 tactactacg gcacaggccg ccgcaaaagt tcagtggctc gtgtattcct gactaaaggt
    17761 acgggtcaaa tcatcgtaaa cggccgtccc gttgacgaat tcttcgcacg cgaaaccagc
    17821 cgtatggttg tccgccaacc tctggttctg actgaaaacg ccgaatctct cgacatcaaa
    17881 gtaaatgttg tcggcggcgg cgaaaccggc cagtccggcg caatccgcca cggtattacc
    17941 cgtgccctga ttgattttga cgctgctttg aaacctgcct tgtctcaagc cggtttcgtt
    18001 acccgcgatg cgcgcgaagt cgaacgtaaa aaaccgggtc tgcgcaaagc acgccgtgca
    18061 aaacaattct ccaaacgttg attttggaaa ttcaaaaaac cctgcttatc gcagggtttt
    18121 ttatttgtaa caggcggttt cccattggta atctaaagat tacggattgg gcaaaaatca
    18181 aaaacagcac cagtatcgac tacacggacg aactgaccgt cagggatggc tgttactgga
    18241 gaggacatcg tatttctact ttgtaaaata acttttaatt tatttagagt tattgaaaaa
    18301 atagaacggc tatttcaact agcctataaa aatggcaatc tattagggtt agcataggtg
    18361 ttaccgccgc cgttcaagcc gatcagtaaa ggaacagttt cctagttttt agctaacctg
    18421 tattatataa tggggagaat atcttaaata accaagcatc cttaatcata atttagagaa
    18481 attatttttt cagagtctaa cccttttaat atgattaact tattaacttt atctatttcc
    18541 cagtctgata ttatatcggt agatactgaa ttctctctta ttaaattttt atctgtaata
    18601 attgcaccca actcatgaat aatacaaatt ttttgattat ccaataaata aatatcaaaa
    18661 actattccat ctatatttag tatgttaact tttgaatttt gtatagaaat acaaaaaacg
    18721 actgaatcaa atccgataaa aatattttta tcatcaagca ttaatacttt tgggggaata
    18781 cttatgcaag aaacagctaa tccaatacct atattgaatt ctttataaaa agaaagaata
    18841 aaacatcgat aaatctcttt tcctccctct tgtagaataa tatatttttg ataggtgttt
    18901 attaaatagt cttctaactc tcttatggaa ttataaggat atttcgttaa tctcattatt
    18961 ttgttccatg tctaaaacgt cctgttgatc catatatttc acccgttatg agtctgttcc
    19021 catctcatat agtttcaagt ttaacaaccc cttgaggcta tgttaaaaga gtttttctaa
    19081 taactattcc gttttcttaa atataaaaaa atagaaaaat agcccgggta tagtttgaac
    19141 aagggaaatc agaccggttc caactcactt aatccgggca agtccgcgaa gccgcgttca
    19201 cgtatgattt ggcaaaagtt gttcagataa cttttatctg tgtcttcagt gcggatggcg
    19261 gcatacagtt tgctttgcag tccgtcggca gtaatttggc ggtggacgac atagcctttt
    19321 tcaaggtagg gcatgactgt ccaataggga agggcggcaa tgccacgtct gctggcaacc
    19381 agttggataa tggcgatggt cagctcgctg tgccggcgcg gcgggttgat gtttttcgga
    19441 atcaggattt ttttgggtaa atccagcatt tcatcgggaa cgggataggt aatcagggtt
    19501 tccccgataa agtcttccgc cgtccaaacg tttttggcgg caagcggatg gtccggggcg
    19561 caaatgccga ccatttcgta ggcaaacagt gggtggaagc tgatgctgct ttgtttttcg
    19621 gcttcggaga caatggcgag gtcggcgcgg tgttgcagca gcagcccgac gggatccgct
    19681 tggaatcctg atacgatatc cagttcgact tggggccaca tcgggcggaa ttcgcccatg
    19741 gcgggcatca gccagtcgaa acaggtatgg cattcgacgg caatccgcag ctctcccgcc
    19801 tccccttccg tgattcgcgc caaatctcgt tctgcaacag caacttgagg tatcagttcg
    19861 tgggcgaggc gcagcagcct ttcgcccacc ggggtaaagc gcaggggcgt ggatttgcgt
    19921 tcgaacagcg gcgtgccgta gtggttttcg agcatacgga tctggtggga aagggcggat
    19981 tgggtaagga aaacccgttt ggcggcaagg gagacgctgc cggtttcttc aagtgccagc
    20041 agggttttga ggtggcgcaa ttcgataatg gaatccatgg ggcttcagac ggcatattga
    20101 accggcgcat attaaaataa ttcatatgcg ggtgcaaatc ggaaaaatgg gcgtttcggg
    20161 catttgcggt tagaatgccc gcttgtttgg aaatgacagg aaaaatatgg ttttgcgcag
    20221 agatttttta gcctggtgcg acgaaacgtt gcagacggca tcgtttaaag attacgcccc
    20281 caatggtttg caggttgaag ggagggaata tatcgggaaa atcgttacgt cggtaacggc
    20341 aagcagggca gcgattgatt ttgctgtgga gcagaaggca gatttgcttt tggtacatca
    20401 cggtatgttc tggaaaaacg agctgccgac cgttaccggt tggaaaaaag aacggattgc
    20461 cgcactgtta cggcacgaca tcaatatggc aggctaccat ctgcccctgg atgtgcatcc
    20521 catactgggc aacaatgccc aactcgccga cagattgggt tttgcgacag aaaaacggtt
    20581 cggcgaacaa aacctgctca actcgggcag cctgaaacaa gccaagacac tcggcgcatt
    20641 ggcggcgcat attgaaacag ttttgcaacg taagcctgtc gccatcggca atcccgaacg
    20701 cgaaatccga cgggttgcat ggtgtaccgg cggtgcacag ggcttttttc agacggcaat
    20761 agatgaaggc gtcgatctgt atctgacggg tgaaatttcc gaagcccaat accacctcgc
    20821 caatgaaacg ggtacggctt tcatttcggc agggcatcac gcgacggaac gttacggcgt
    20881 acgcgcgctg gcagaatcgg cggcagaggt tttcgggttg gaagtgtgcc attttgacga
    20941 aaacaacccg gcttgagtct tgagaaatat cataaaactt taccttattt taaataatgc
    21001 tttgagtatc actgcaaact gggtaaaatt gcaatgttta atggctcggc attcccaaaa
    21061 tattaggacg actgaataat ggataatcaa gaaatcaaca acggccgccg ccgtttcctg
    21121 acacttgcga cctgcggcgc aggcggagtg gcagcattgg gtgtggcaac gccgtttgtg
    21181 gccagttttt tcccttcgga aaaagccaag gccgccggtg ctgccgtcga agtggatgtc
    21241 agtaaaatcg aagcgggtca gctgctgacc gccgagtggc aaggcaaacc gatttgggtg
    21301 ctcaaccgta cagatcagca gcttaaagac ctgaaaggcc tgaacggcga acttaccgat
    21361 cccaattccg atgcggaaca gcagccggag tatgctaaaa acgagacccg ttcgattaag
    21421 ccgaacatcc ttgtcgccat cggtatctgc acccatttgg gctgctcgcc caccttccgt
    21481 cccgacattg cccccgccga tttgggcgca gactggaagg gcggcttctt ctgcccgtgc
    21541 cacggttcga aattcgactt ggccggccgc gtatataaag gtgttcctgc cccgaccaac
    21601 ctggttgtcc cgccatataa atacttgagc gacacaacta tcttggtggg cgaagactaa
    21661 gaataaggaa cgataattat ggcaaaccaa accaatagca aagcaaaagc attgttaggc
    21721 tggatggacg ctcgcttccc cctgtctaaa atgtggaatg agcatttggc gcaatactat
    21781 gcgccgaaaa actttaattt ttggtactat ttcggctctt tagccctgct cgtcctcgtg
    21841 attcaaatcg tcagcggtat tttcctgacg atgaactaca aaccggacgg caacctgaat
    21901 gcctaccatc tgcctgctgc ctttaccgca gtagagtaca tcatgcgcga cgtgtccggc
    21961 ggttggatta tccgctacat gcattccacc ggcgcatctt tcttcttcat tgttgtttat
    22021 ctgcacatgt tccgtggttt gatttacggt tcatataaaa aaccgcgcga attggtgtgg
    22081 attttcggtt ctttgatctt cttggcattg atggcggaag cctttatggg ctacctgctg
    22141 ccttggggtc agatgtcctt ttggggtgcg caggtgatta tcaacctgtt ctccgccatt
    22201 cccgtcatcg gtcctgattt gtccacttgg attcgcggcg acttcaacgt ttccgacgtt
    22261 accctgaacc gcttcttcgc cctgcacgtt atcgccgtac cgctggtatt gctcggttta
    22321 gtcgtggcac acatcatcgc actgcacgaa gtgggctcta acaaccccga cggtgtggaa
    22381 atcaagaaaa acaaagatga aaacggcatt ccgcgcgacg gcatcccgtt ccacccttac
    22441 tacactgtta aagacatcct gggcgtcgtt gtattcctga ttgtcttctg tgccgtattg
    22501 ttctttgccc ctgaaggcgg cggctacttc ctggaagcgc cgaacttcga tgcggcaaat
    22561 gcgctgaaaa cacccccgca cattgcgccg gtatggtatt tcacaccgtt ctacgccatt
    22621 ttgcgtgcga ttccttcctt cttgggaacg caggtttggg gtgtaatcgg tatgggcgcg
    22681 gcggtcgtgt tgattgccct gctgccttgg ctggacaggg gcgaggtgaa atccgtccgt
    22741 taccgcggtc cgattttcaa aaccgcattg gtcttgttca tcatttcctt tatcggtttg
    22801 ggtattttgg gcgcgatggt ggcaaccgat acgcgtactt tggtcgcgcg catcctgtcc
    22861 ttcgtctatt tcgcattctt cctgggtatg ccgttctata ccaagctgga taaaaacaaa
    22921 ccggttcccg aacgcgtaac catgagcacc gccaaacaaa aaatcatgtt ctttgtttac
    22981 gtcggcatta ccgccgtggg tgcttacctg tttgcaacca acatctgatg agggcagtca
    23041 aaatgaaaca agctatgaaa aattggtttg ctgccttgct gtcggcagta cccatgggtg
    23101 cggcatttgc ttcgggcggt catgcacact atgagaaagt cgatatcgat ttgcgcgacc
    23161 aagtcagcct gcagcgcggc gcacaaatct ttaccaacta ctgtttgtcc tgccactcgg
    23221 caagcggtat gcgtttcaac cgtttgaaag acatcggttt gactgacgaa gaaatcaaga
    23281 aaaacctgat gtttaccacc gataatgtcg gcgatgtcat gcattcggcg atgaacccga
    23341 aagatgcggc aaaatggttt ggtgctgctc cgcccgattt gacattgatt gcgcgttcca
    23401 aaggtgcaga ctacctttac gcttatatgc gcggcttcta taaagatccg acccgtccga
    23461 gcggctggaa caatactgta ttcgataaag tcggtatgcc ccacccgttg tgggaacagc
    23521 aaggcgttca agccgttgag ttggatgcca aaggtcagcc ggttatggta aaagacgaac
    23581 acggcgagat gaagcctaag ctgtattggg aatctaccgg tttgcacagc cgccgtctgc
    23641 ctaacggcaa agtgatccag aaagagtacg acgcatatgt acgcgatttg gtcaattacc
    23701 ttgtgtacat gggcgaacct gcacaactgc aacgcaaacg tataggctat gtcgtgatga
    23761 ttttcctatt tgcggttatg ctgcctttgg cgtatttcct gaaaaaagaa tattggaaag
    23821 acgtacacta agcgtttgga acaaaagggc aaatccttta gggtttgccc ttttttcatt
    23881 ttgcctgccg tttgaaaagc ctgaatccgt atgccgtctg aacatagctg caacatttca
    23941 gacggcatca ttctaaaaat gtcaggcatc gggaacttaa tcagggtttg tggcagattg
    24001 atattgaatt aggaaaaggt ctgaaaccgc aagcagcatg gtaaggcgat gtcgaacagg
    24061 tttaaagcca atccgctata ttttttggag ctgcaccgtg caaacaggta gaatggcggc
    24121 ggttttgtcc ggtgtgaaaa tgtttgaata ttaggatgat tgatggagct gatgactgtt
    24181 ttgctgcctt tggcggcgtt ggtgtcgggc gtgttgttta catggttgct gatgaagggc
    24241 aggtttcagg gcgagtttgc cggtttgaac gcgcaattgg cggaaaaggc ggcaagatgt
    24301 gattttattg aacaggcaca cgcagaaatt gcatcagaat tggccgcttt ggatgggaaa
    24361 tacgagcatt tgcaggacga aaattatgct ttgggcaacc gtttttccgc agccgaaaag
    24421 cagattgccc atttgcagga aaaagaggcg gaatcggttc ggttgaagca gtcgtatatc
    24481 gatttgcagg aaaaggcgca gggtttggcg gttgaaaacg aacgtttggc aacgcagctc
    24541 gggcaggaac ggaaggcgtt tgccgagcaa tatgcgctgg aacgccaaat ccgccaaaga
    24601 gtcgaaaccg atttggaaga aagccgccaa actgtccgcg acgtgcaaaa cgacctttcc
    24661 gatgtgggca accgttttgc cgccgccgaa aaacagattg cctatttgca ggaaaaagag
    24721 gcggaagcgg agcggttgag gcagtcgcat accgagttgc aggaaaaggc gcagggtttg
    24781 gcggttgaaa acgaacgttt ggcaacgcaa atcgaacagg aacgccttgc ttctgaagaa
    24841 aagctgtcct tgctgggcga ggcgcgcaaa agcttgagcg atcagtttca aaatcttgcc
    24901 aacacgattt tggaagaaaa aagccgccgt tttaccgagc agaaccgcga gcagctccat
    24961 caggttttga acccgttgaa cgaacgcatc cacggtttcg gcgagttggt caggcaaact
    25021 tatgataaag aatcgcgcga gcggctgact ttggaaaacg aattgaaacg gctccaaggg
    25081 ttgaatgcgc agctgcacag cgaggcaaag gccctgacca acgcgctgac cggcacgcag
    25141 aacaaggttc agggcaattg gggcgagatg attctggaaa cggttttgga aaattccggc
    25201 cttcagaaag ggcgggaata tgtggttcag gcggcatccg tccgaaaaga ggaagacggc
    25261 ggcacgcgcc gcctccagcc cgacgttttg gtcaacctgc ccgacaacaa gcagattgtc
    25321 attgattcca aggtttcgct gacggcttat gtgcgctaca cgcaggcggc ggatgcggat
    25381 gaggcggcac gcgaactggc ggcgtatatc gccagcatac gcgcgcatat gaaaggcttg
    25441 tcgctgaagg attacaccga tttggaaggt gtgaacacat tggatttcgt ctttatgttt
    25501 atccctgtcg aaccggccta cctgctggcg ttgcagaatg acgcgggctt gttccaagag
    25561 tgtttcgaca aacggattat gctggtcggc cccagtacgc tgctggcgac tttgcggacg
    25621 gtggcgaata tttggcgcaa cgagcagcaa aaccaaaacg cgctggcgat tgcggacgaa
    25681 ggcggcaagc tgtacgacaa gtttgtcggc ttcgtacaga cgctcgaaag cgtcggcaaa
    25741 ggcatcgatc aggcgcaaaa cagctttcag acagcattca agcaacttgc cgagggacgc
    25801 gggaatctgg tcggacgcgc cgagaaactg cgtctgttgg gcgtgaaggc aggcaaacag
    25861 cttcaacggg atttggtcga gcgtgccaac gaacagacgg catttgcttt gggcaaacct
    25921 tcggaacagg aagcggctga cgaagccgaa taggaaaggc ggatgcgttt cagacggcat
    25981 cagtgcagat gatgccgtct gaagactttg ggaagtcggt tagaaaaacg gattgtgccg
    26041 tttttcgtgt ccgatggaag tcatacgccc gtgtccggcg acaacttgca cggtttcggg
    26101 gaaggcgaat aatttgttgc ggatattgtt gattaagtcg gcgtggttgc cgcgcggaaa
    26161 atcggtcctg cctatggttt cgtaaaacag cacgtcgccc gcaatcagca gctccgcttc
    26221 ggcacaatag aaaaccacgt gtccgggcgt gtgccccgga atatgcagca cttggaaggc
    26281 atagcgtccg accgtgagcg tttcgccttc ttcgagccaa cggttcggcg caaaggcggg
    26341 cgagacgggg aatccgtatt gcgcggtggt ttgcggcagc gattggagca ggaattcatc
    26401 ttcgcggtgc gggccgagga cggggacttt atgcgtttcc agcatctcga ccacgccgcc
    26461 cgcgtgatcg agatgaccgt gcgtcagcca gattgccgtg agcgtgagtt tgcggtttgc
    26521 caacgcttgc agcaggaacg gcacgtcgcc gccgacatcg gtcaggacgg cttcgccgct
    26581 ttcgtcgtcc caaatcaggg tgcagttttg gcggaagggg gtaacgggga gaatttcgta
    26641 acgtagggtc atcggcgtgt tcctaaaacg gtttttcaga cggcatcggg tttgccgttt
    26701 gttttatggc ggtttgccgc ctgttttgat attgggggaa tcaggtgatt aagaagataa
    26761 tcggcggcat cataccgatt tttacggcgg ttttcatccc tgcatcggca ggcgcggcgg
    26821 atttgatgct ggcgcaggaa tacaaagggc aggacattgc cggctgggcg atgagcgaga
    26881 agctcgacgg cgtgcgcgcc tattgggacg gaaagcacct gataagccgt cagggctacg
    26941 cgtttacgcc ccccaaaggc tttaccgcgc agtttccgcc ttatcctttg gacggcgaat
    27001 tgtatagcgg gcgcggtcag ttcgagcaga tttccgctac cgtgcgttct gtttcttcag
    27061 actggcgcgg catccgcctg cacgttttcg acgtacccaa ggcgcagggc aacctctacc
    27121 aacgtttggc agtcgcaacg cagtggctga aaacgcatcc gaacgcgccg attaccatca
    27181 tcccgcaaat caaagtgcgc gaccggcggc acgcgatgga ctttttaaaa caaatcgaag
    27241 cgcagggcgg cgaaggcgtg atgctgcgcc agcccgaatc ccgttacagc ggcggcagga
    27301 gcagccaatt attgaagctg aaaagccaat acgacgacga atgcacggta acgcggcact
    27361 atgagggcaa agggcgaaac gccggacggc tgggcgcggt cggctgcaaa aaccgacacg
    27421 gcgaattccg catcggcagc ggtttcaagg acaaagaccg cgacaacctg cccaaaatcg
    27481 gcacgctgat tacctaccgt taccgtggct ttacgcggaa aggcacgccg aaatttgcca
    27541 catttgtgcg cgtgcgtacc gaccgctgag caggacagtt cagacggcat ccgatacttt
    27601 ggtttataat tttcctttta ccgaccgatt ccgacatatg accgatttag aaaccaaacg
    27661 ccttgaaaca caggcgatgc ttgaaaacgc cgatcttttg ttcgaccaag gccaatgccg
    27721 tgccgcactg caaaaagtgg cgtacgagat tacgcgcgat ttgggggata aatatccgtt
    27781 gctcctgccc gtgatgggcg gggcggtggt gtttacgggg cagttgctgc cgctgttgcg
    27841 ttttccctta gattttgatt atgttcacgt ttcccgttac ggcgacaagc tggagggcgg
    27901 cgcgttcaac tggaagcgta tgcccgatgc ggaacaaatc cggggcaggc acgtcgtcgt
    27961 gctggacgat attttggacg aagggcatac gatgtccgcc attcaagcca aacttttgga
    28021 aatgggtgcg gcaagctgcc gtgcggcggt gttcgccaac aagctgattg acaaacccaa
    28081 gcctatccga gccgattatg tcggactgga tgtgccgaac cgttatgttt tcggttacgg
    28141 catggatgcg gcgggctgct ggcgtaattt gggcgagatt tacgcattgg acggaaaata
    28201 aggacgcgat gccgtctgaa ggcctttcag acggcatcgc gcccatacgc cggcaggatt
    28261 aatgaggaac aggacgcatc aatatgatag ggcttttaat catcacacac gaaaccatag
    28321 gcgaagccta ccgcaagctg gcgcatcatt tttttccggg cgggctgcct gaaaacgtcc
    28381 gcatactcgg cgtgcagccg acggaaggcc aagacgacat tatcaacaac gccatttccg
    28441 cgctgcaaga gtttcctgaa aaccacggtg tattgattat gaccgacatc ttcggcgcga
    28501 ccccctgcaa tgccgcccgc cggctcgtgc gcgaaaacaa atcggcgatt ttgaccgggc
    28561 tgaacgcgcc gatgatgatt aaggccgtcc aatattcgcc ggcggcggaa gactttgccg
    28621 cctttaccga atgcgtcagg gaggcggcgg taaaaggcat tttcgccatc acgtccgcgc
    28681 ccgaagattt ggtgtgccgg cgcagcggcg atgccgtctg aagaagcggc agggcaggaa
    28741 aacattttaa acggcgtctg ctgccgatat atagcggatt aacaaaaatc aggacaaggc
    28801 ggcgggccgc agacagtaca aatggtacgg aaccggttcg cctggtgctt cagcacctta
    28861 gggaatcgtt ccctttgagc caaggcgggg caacgccgta ctggtttttg ttaatccact
    28921 atacataaca cgggaaccga aatgctcaaa caatccatcg aaatcatcaa caaactcgga
    28981 ctccacgccc gcgcgtccag caagttcacc caaaccgcgt cccaattcaa aagcgaagtc
    29041 tgggttacga aaaacggcag ccgcgtcaac ggcaaaagca ttatggggct gatgatgctc
    29101 gcagccgcca agggtacggt catcgaactg gaaaccgacg gcttggacga agccgcagcc
    29161 atgaaggcat tgaccgactt aatcaacgac tacttcggcg agggcgaata atgagtatcg
    29221 tgctgcacgg cgtggcggcg ggcaaaggca ttgccgtcgg ttgcgcccac ctgattgcgc
    29281 gcggtacgga ggaagtgccg cagtatgatg ttgctcaggc ggacaccgat gccgaagccg
    29341 aacgtttcga tgccgccgtc aaagccacgc gcaaagagtt ggaacagctc cgcagcgcga
    29401 ttcccgaaaa cgccccgacc gagttgggcg cgttcatctc gctgcacctg atgctcttga
    29461 ccgatgttac cttgtcgcgc gaacccgtcg atattttaag ggaacaaaaa atcaacgccg
    29521 agtgggcatt gaagcagcag agcgacaaac tcgccgccca attcgacaat atggacgatg
    29581 cctatttgcg cgaacgcaag caggatatgc tgcaagtcgt ccgccgcatc cacaacaacc
    29641 tgatcgggca gggcaacgag ttggaggttg ccgacaacct gtttgacgaa accgttctga
    29701 ttgcaaacga cctttcaccc gccgacacgg ttttgtttaa agagcagcgc attgccgcct
    29761 tcgttaccga tgccggcggc cccaccgggc atacggcgat tttgggcagg agcttggaca
    29821 tcccgtccgt cgtcgggctg cacaacgcgc gcaaactgat taccgaaggc gaaacggtca
    29881 tcgtggacgg catcaacggc gtgttgatta tttcgccgga tgagtcggtg ttgaacgaat
    29941 accgccgccg cgcccgcgaa taccgcagcc acaaacgcga tttgaacaag ctcaaaaaaa
    30001 ccgccgccgc caccgccgac ggggtctgca tcgagcttgt gggcaatata gaatccgccg
    30061 aagacgtgaa accgctgcac aacctcggcg cagacggcat cgggctgttc cgcagcgagt
    30121 ttctttacct gaaccgcgat acgatgccgt ctgaagacga gcagtacgaa gtgtacagcg
    30181 cgattgtcaa aaaaatgaag ggcaaaagcg taacgatacg gacagtcgat ttgggtgtgg
    30241 acaaaaatcc gcgctggttc gggaaaaaca gcacgcccaa cggcagcctc aaccccgcgc
    30301 tgggcatgac cggcatccgc ctgtgccttg ccgaaccggt catgttccgc acgcagatgc
    30361 gcgccatcct ccgtgcggcg gtacacggtc ccgtccgcat gatgtggccg atgattacct
    30421 ccgtatccga agtgcgccag tgcctcatcc acctcgacac cgcgcaacgc cagcttgccg
    30481 aacgcggcga tgccttcggc gaagtcggca tcggctgcat gattgaaatt ccgtctgccg
    30541 cgctgaccgt cggcagtatt ttgaaactgg tcgatttcat ctccgtcggt accaacgatt
    30601 tgatccaata tattctgtcc gtcgatcgcg gcgacgacag cgtcagccat ctctaccaac
    30661 ccggacatcc ttccgtgctg aaaatgctgc aacacgtcat ccgtaccgcc aaccgcatgg
    30721 acaaagacgt gtccgtatgc ggcgagatgg cgggcgatac cgcgtttacc cgcgttttat
    30781 tgggtatggg gctgcgccgt ttttccatga atccgaacaa catcctgccc gtcaaaaaca
    30841 tcattctgca cagcaatgcc ggacagctcg aaggcgatat tgcgaaagtc atccgctgcg
    30901 aagacgaaga gaagtcggaa aagctgatca aacagatcaa cagcgtgtct gtcgcggaag
    30961 aagccgaagt caagggacgg aaataaatat tgttgacaat gcgagcgggt tgcagtcaaa
    31021 taacgccctt aaaaaataca gctgctttgg tcaagaaatg ccgtctgaaa aattttcaga
    31081 cggcattttg tttggcaggc atttctgccg catcatcgga aacggacgat acgcccttca
    31141 tcgacgcgga tgcggaccgt accgtttccg gaaataccgt ccgtatgttg tccgacagtc
    31201 aggtttaagg tcagctcgcc gtgttcggga tggacggcgg aaagccggag cgagtcgggc
    31261 aggcggacga gggacagcag gcggcattcc gttccatgat tgtccaagca cacggcattt
    31321 tgcggaatat ggcggtcatc gtcggtattg ggcagcccca tcagacgggc gacctgcacg
    31381 ccggcaggcg tttgaatcaa ggtttcgggc gtaccgcatt gaaggatttt cccctcgtgc
    31441 atgacggcga tttcgtccgc cgccgtgcag gcctcttcgg gcgaatgcgt taccaaaacg
    31501 gcagggatgc cgcccttgcg gatgcgttcg gcggtcatac ggcgcagccg gtcgcgcaaa
    31561 tgcgtgtcca aactggaaaa cgattcatcc agcaacagca gggaagggcg gacaaccaaa
    31621 gcgcgcgcca gtgccaaccg ttgcttctcg cctccggaaa gtttttcagg cttgcggtgc
    31681 gcctcgtttt ccagcccgac ttcggcaagt gccgacaagg cgaggcgttc ggcttcggct
    31741 ttcggcattt tttgcatttt caaaccgaat gccgtatttt ccagcgcact catatgggga
    31801 aacagcgcgt aatcttgaaa catcagcgag atacggcgtt tttcgggcgg catacaggta
    31861 atgttttccc cgttcagccg aatttcgccg ccgtccggcc ggacgatgcc cgcaatcata
    31921 ttcagcaggg tggatttgcc gcagcccgac cgccccagta cggcgagtat tttgccgcgc
    31981 ccgacagtca ggcagatgtt gtcggcgacc gttttgccgc cgaagcattt gcagagtccg
    32041 ttcagttcaa gcatggcgca tcctataaac gtatgccgtc cagccactcg gacagcggat
    32101 gggcggcatc aatcagaccg taacggtaaa acgcgtccag cgtaaccagt tgcgcgtcgt
    32161 gcatcatgtt tttcgacaac atggcatcca ataggccgcc aatgtccatc ttttcaaaac
    32221 ccgctacctc gccatcctga ttttcaggca ggaaggtttc gggcaggacg gcatcgaaca
    32281 catacaggat ttcattgtgc acacctcggc tgacggggcg aaggctgtgc agccgcgata
    32341 ctgggcggat gagcggaaac agcgttttat ccaaaccggc ttcttcgctg ctttcgcggc
    32401 acacggcttc agacggcatt tcgccgccgg aaacaccgcc gccggcaata ttgtcgagct
    32461 tgccgggatc gactgctttg tgcggactgc gcctgcctat ccaaaaatgc catctgccgt
    32521 tcgattcgac caaaccgttg agatggacgg cgcggctgag tagtccgaac ggacggaaag
    32581 cggcgcgttc gagcgtgaac aaggggttgc cgccgccgtc ggtcaggtcg aaacactcgt
    32641 tgcgccatcc gtgaagcagc cccgccttgt tccatgtgcg ggcgaggtgc tgcaagcgtc
    32701 cgcccatatc cggccagccg tccgcattca gaaaaatgcc gtctgaagac tcggagcagc
    32761 ctgcctccca gtcttttttg atgcgctcag cccattccgg cgacagattg cccaaaggaa
    32821 gacggttcag atacagcgtt ttccagcaac tttcggcacc gtaacttgct tttgcccgct
    32881 cgaacagggc atcaaggtct tgtttgctga cggattcggt aaaacggacg gtcggcatag
    32941 ccggctttct tgaacacaat gcggaagatt gtagccaaac cgcctgaatt tgcaaccgca
    33001 aacagacggc ggcctgccgt tccgatattg cggcaggaag gaaaatccgg caaaaaacgg
    33061 aggcggcagg aaacaggaaa aacaaaaccc cctcggcatt gaaccgaggg gtttgatatt
    33121 tggttgcggg agcaggattc gaacctacga ccttcgggtt atgagcccga cgagctacca
    33181 tgctgctcca tcccgcgtca gaagatgaaa ctatacggca gattttttat gttgtcaaac
    33241 attatttccg tgaaaaatca taaagttttc cgtccggcgg cttatgcctg atttgaaata
    33301 tcattttctt tacaaaagtt catgttcgtg atttaatttt ggttaacatc gaaacagggg
    33361 tgctgcctga tgttcaggcg gctgagaaat accctttaca cccgatcggg ataatacctg
    33421 cgtggggagt tttcacggat tctgtctttc agacggcatt ggttttcaaa tgccgtctga
    33481 aaacgcaaaa cgctcctgtt tctttattct aaacgagaaa acaggagcat tttttatgac
    33541 tacgccaaaa aaaaccgcca aaatttccgg caacgaagcg cgcgaacttt ccgacttgag
    33601 cgaagacatc ggcatccgct tcaaatatcc gaactccgaa cgcgtgtatc tgcaaggcag
    33661 ccgcgacgac atccgcgtac ctttgcgcga aatccgtcag gacgacacct acacggcgca
    33721 aggtacggaa gccaacccgc ctattccggt gtacgacacc agcggcgcat acggcgatcc
    33781 ggcggcgcac atcgacctga aacaaggcct gccgcacatc cgcacggcgt ggctggacga
    33841 acgcggcgat accgaaatcc tgcccaaact ctccagcgaa tacggcatcg aatgcgcaca
    33901 cgatccgaaa accgcccatc tgcgcttcaa ccaaatcacc cgcccgcgcc gtgccaaagc
    33961 cggccgcaat gtaacccagc tccattacgc ccgccaaggc attatcacgc ccgaaatgga
    34021 gttcgccgcc atacgcgaac gcatgaagct ggacgagctt ttcagacggc ctgaatacgc
    34081 caagctcttg aaacagcaca cagggcaaag tttcggtgcg aacatcccga cccgtcccga
    34141 ccaaatcacc cccgaattcg tgcgccaaga aatcgccgcc ggacgcgcga ttatccccgc
    34201 caacatcaac caccccgaac tcgaaccgat gattatcggc cgcaacttcc gcgtcaaaat
    34261 caacggcaac ttgggcaatt ccgccgtaac ctccagcctg accgaagaag tcgaaaaaat
    34321 ggtgtggtcg ctgcgttggg gcgcggacac gattatggac ttatccacag gcgcgcacat
    34381 ccacgaaacg cgcgaatgga ttatccgcaa cgcgcccgtc cccatcggca cagtgccgat
    34441 ttaccaaact ttggaaaaaa ccggcggcat cgccgaagat ttgacttggg atttgttccg
    34501 cgacacctta atcgagcagg cggaacaagg cgtggactat ttcaccatac acgcgggcgt
    34561 gttgctgcgt tatgtgccga tgaccgccaa ccgcctcacc ggcatcgtat cgcgcggcgg
    34621 ttcgattatg gcgaaatggt gtttggcaca tcatcgggaa aacttcctct acacgcattt
    34681 cgacgaaatc tgcgaaatca tgaaggcgta tgacgtatcg ttcagcttgg gcgacggcct
    34741 gcgccccggc tgcattgccg atgccaacga cgaatcccaa ttcgccgaat tgcacacctt
    34801 gggcgaattg accgataaag cgtggaaaca cgacgtacaa gtcatgatcg aaggccccgg
    34861 ccatgtgccg ctgcaacgcg tcaaagaaaa tatgaccgaa gagttgcaac actgctttga
    34921 agcaccgttt tacacactcg gcccgctcgt taccgacatc gcccccggct acgaccacat
    34981 cacttcgggc ataggcgcgg ccaatatcgg ctggtacggc acggcgatgc tttgttacgt
    35041 tacccccaaa gaacatttgg ggctgcccga caaagaagac gtgcgcaccg gcatcatcac
    35101 ctacaaactc gccgcccacg ccgccgacct cgccaaaggc tggccgggcg cgcaattacg
    35161 cgacaacgcc ctgagcaagg cgcgtttcga attccgctgg cgcgaccaat tccgcttaag
    35221 cctcgacccc gaacgcgccg agagcttcca cgacgaaacc ctgcccgccg aaggcgcgaa
    35281 aatcgcccat ttttgctcaa tgtgcggccc caaattttgc tcgatgaaaa tcacgcagga
    35341 agtgcgcgac tacgccgaca agcaaaaagc ccaacggcag ggtatggagg aaaaagcggt
    35401 cgagttcgtc aaaaaaggcg cgaagattta cagttgaaac gtcaagcaaa aaatgccgtc
    35461 tgaagcccgg aaaaaaggct tcagacggca ttttttcgct tgtgaaaatg attaaacaac
    35521 cgatcattaa agtaattttt aaaattttta tattcacata gatgggaatg atggatttag
    35581 ggttctgatt ttgttttgag agaatgaagg aatttgagat tgtgggcgat tatcgggaaa
    35641 aatagaatct ttc
//
LOCUS       DAWTCI010000012        35096 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWTCI010000012 DAWTCI010000000
VERSION     DAWTCI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35096
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.012"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            58..444
                     /locus_tag="WM481_000617"
                     /pseudo
     CDS             58..444
                     /locus_tag="WM481_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(827..1717)
                     /gene="sucD"
                     /locus_tag="WM481_000618"
     CDS             complement(827..1717)
                     /gene="sucD"
                     /locus_tag="WM481_000618"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFA9079995.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLDLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="WM481_000619"
     CDS             complement(1728..2894)
                     /gene="sucC"
                     /locus_tag="WM481_000619"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFA9079996.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREKAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFAKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(2966..3253)
                     /locus_tag="WM481_000620"
     CDS             complement(2966..3253)
                     /locus_tag="WM481_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9079997.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAVMYLLARMWLPFSK"
     gene            complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="WM481_000621"
     CDS             complement(3373..4806)
                     /gene="lpdA"
                     /locus_tag="WM481_000621"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9079998.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGAKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="WM481_000622"
     CDS             complement(5067..6248)
                     /gene="odhB"
                     /locus_tag="WM481_000622"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFA9079999.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATVAAEAPAAAPAEAAPAAVPAA
                     AQNNAAMPAAAKLAAETGVDVNVLQGSGRDGRVLKEDVQNAAAKPAAAVAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVTALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPVRLLLD
                     L"
     gene            complement(6348..9176)
                     /locus_tag="WM481_000623"
     CDS             complement(6348..9176)
                     /locus_tag="WM481_000623"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFA9080000.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALRFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQVEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREKIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLLTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRNMGEGLGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="WM481_000624"
     CDS             complement(9381..10664)
                     /gene="gltA"
                     /locus_tag="WM481_000624"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9080001.1"
                     /translation="MSKSIKLNVPGRAGLELPVLEASIGHDVVDIRGLTKNTGLFAFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDITNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(10776..11024)
                     /locus_tag="WM481_000625"
     CDS             complement(10776..11024)
                     /locus_tag="WM481_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFA9080002.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALIHGHSETDKGHLIPMLEKIRRA"
     gene            complement(11028..11735)
                     /locus_tag="WM481_000626"
     CDS             complement(11028..11735)
                     /locus_tag="WM481_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFA9080003.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRSLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="WM481_000627"
     CDS             complement(11855..13618)
                     /gene="sdhA"
                     /locus_tag="WM481_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFA9080004.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVQEIMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="WM481_000628"
     CDS             complement(13621..13962)
                     /gene="sdhD"
                     /locus_tag="WM481_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFA9080005.1"
                     /translation="MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLVVLFALPKEY
                     PAWQAFFSQAWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSVKVIWG"
     gene            complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="WM481_000629"
     CDS             complement(13956..14333)
                     /gene="sdhC"
                     /locus_tag="WM481_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFA9080006.1"
                     /translation="MSVKLRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGILWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(14620..16023)
                     /locus_tag="WM481_000630"
     CDS             complement(14620..16023)
                     /locus_tag="WM481_000630"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9080007.1"
                     /translation="MKKIQADIVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMRRVKSERDRFVGFVV
                     TDVEEWPADKRIMGSAKFIDEHTVQIDDHIQIAAKSFVIATGSRPVILPQWQSLGDRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIDKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGSGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(16272..17009)
                     /locus_tag="WM481_000631"
     CDS             complement(16272..17009)
                     /locus_tag="WM481_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFA9080008.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAICCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKVKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(17288..19564)
                     /gene="metE"
                     /locus_tag="WM481_000632"
     CDS             complement(17288..19564)
                     /gene="metE"
                     /locus_tag="WM481_000632"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFA9080009.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLDVAKDLRE
                     KNWKHQAAANADYVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLPLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQTLGLKAKPTVVGPL
                     TFLWVGKEKGSVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLNKVSAKILLGTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFAGY
                     DKVLSAGVIDGRNIWRANLNKVLETVGPLQAKLGERLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPVNADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSASDYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQNLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLAAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(19700..20578)
                     /gene="metF"
                     /locus_tag="WM481_000633"
     CDS             complement(19700..20578)
                     /gene="metF"
                     /locus_tag="WM481_000633"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFA9080010.1"
                     /translation="MNYAKEINALNNSLSDLKGNINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASHDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPKWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGARP"
     gene            20775..21050
                     /locus_tag="WM481_000634"
     CDS             20775..21050
                     /locus_tag="WM481_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFA9080011.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAGTHGKTMVWTDGKE
                     YPLFSLDTSSSSHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            21050..21175
                     /gene="ykgO"
                     /locus_tag="WM481_000635"
     CDS             21050..21175
                     /gene="ykgO"
                     /locus_tag="WM481_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFA9080012.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKSRQR"
     gene            complement(21261..21479)
                     /locus_tag="WM481_000636"
     CDS             complement(21261..21479)
                     /locus_tag="WM481_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080013.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRVLREVAEISAWVLLG
                     AAAATLFWYLFMLYFKHIPDSYRRKNAV"
     gene            21622..22761
                     /locus_tag="WM481_000637"
     CDS             21622..22761
                     /locus_tag="WM481_000637"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFA9080014.1"
                     /translation="MSQNTSVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPMVTVKDWVKSQAALADYLGIEQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYSVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVTAYMNNVDKDCRL"
     gene            22758..23339
                     /gene="metW"
                     /locus_tag="WM481_000638"
     CDS             22758..23339
                     /gene="metW"
                     /locus_tag="WM481_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFA9080015.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALAEHKKCSGYG
                     IEIDTDNVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RLAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            23456..23647
                     /locus_tag="WM481_000639"
     CDS             23456..23647
                     /locus_tag="WM481_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080016.1"
                     /translation="MKNKTSSLPLWLAAIMLAARSPSKEDKTKENGASAASSSASQSD
                     LQPAASAPDNVKQAESAPL"
     gene            23690..24253
                     /locus_tag="WM481_000640"
                     /pseudo
     CDS             23690..24253
                     /locus_tag="WM481_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
     gene            24251..24358
                     /locus_tag="WM481_000641"
                     /pseudo
     CDS             24251..24358
                     /locus_tag="WM481_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(24434..24994)
                     /gene="efp"
                     /locus_tag="WM481_000642"
     CDS             complement(24434..24994)
                     /gene="efp"
                     /locus_tag="WM481_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFA9080017.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVDYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(25037..26188)
                     /gene="earP"
                     /locus_tag="WM481_000643"
     CDS             complement(25037..26188)
                     /gene="earP"
                     /locus_tag="WM481_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFA9080018.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPFVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLN
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYRE
                     AVRFDTEALRRRLVLPEKNAPEWLLFGYRGDVWAKWLDMWQQAGSLMTLLLAGAQIID
                     SLKQSGVIPQNALQNDGGVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     TQLAGKPFFWHIYPQDENVHLDKLHAFWDKAYGFYTPETASVHRLLSDDLNGGEALSA
                     TQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(26207..26485)
                     /locus_tag="WM481_000644"
     CDS             complement(26207..26485)
                     /locus_tag="WM481_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080019.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFETIKHHLKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
     gene            26836..27777
                     /gene="miaA"
                     /locus_tag="WM481_000645"
     CDS             26836..27777
                     /gene="miaA"
                     /locus_tag="WM481_000645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFA9080020.1"
                     /translation="MPTPKAFTLLGPTACGKTALALKIAETLPVEIISLDSALLYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLTGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGRPMSAHLNGQPEHTLPFELYTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGE"
     gene            27781..28500
                     /locus_tag="WM481_000646"
     CDS             27781..28500
                     /locus_tag="WM481_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFA9080021.1"
                     /translation="MLATPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTPPQEMSQAHKDCLLAELKNHPPVAAFPPQEEMGYFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIITSAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            28505..28993
                     /locus_tag="WM481_000647"
     CDS             28505..28993
                     /locus_tag="WM481_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFA9080022.1"
                     /translation="MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGILWTVGLISLGGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILLILLVKYIAY
                     LF"
     gene            29059..29811
                     /gene="rlmB"
                     /locus_tag="WM481_000648"
     CDS             29059..29811
                     /gene="rlmB"
                     /locus_tag="WM481_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFA9080023.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSITELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDIGGNADLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNKKA"
     gene            complement(29873..31247)
                     /locus_tag="WM481_000649"
                     /pseudo
     CDS             complement(29873..31247)
                     /locus_tag="WM481_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
     gene            31627..32502
                     /locus_tag="WM481_000650"
     CDS             31627..32502
                     /locus_tag="WM481_000650"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFA9080024.1"
                     /translation="MLQGSLVALITPMNQDGSIHYDQLRQLIDWHIENGTDGIVAAGT
                     TGESATLSVEEHLSVIEETVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAVPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNVGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGNIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCGPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            32511..33707
                     /gene="bamC"
                     /locus_tag="WM481_000651"
     CDS             32511..33707
                     /gene="bamC"
                     /locus_tag="WM481_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFA9080025.1"
                     /translation="MPSEPFGRHNMTNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIESEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGDKNKDTTMWQPSASDPNLEAAFLTRFMQYLGVDGRQAENALAKKPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTGLALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(33764..34696)
                     /gene="pip"
                     /locus_tag="WM481_000652"
     CDS             complement(33764..34696)
                     /gene="pip"
                     /locus_tag="WM481_000652"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFA9080026.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSHPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEGVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTVIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPRLL"
BASE COUNT         8682 a         9481 c         8698 g         8235 t
ORIGIN      
        1 cactatatat agaaggtata tatcctagta ggcgtaatgg cagctattac aaaatgtctg
       61 catcaggttc acgaaacgca agtcgaaagc gtcaaacaac tgattgcccg ttttgaccgg
      121 ctgattgacg aatcggacaa acaaatcgac aaccacaccc gcacgcattt tgacggcaaa
      181 gcccaagtgg cggagcaaat caaaggcatc ggttcgataa cgacggctac gctgatggcg
      241 atgctgcccg aattggggcg gctgtcgcac aaacggatag cgggtttggt cggcattgcc
      301 ccgcacccga gggagagcgg ggaaaccaaa ttcaaaagcc gctgcttcgg cggaaggtct
      361 gcggtgcgta aggcgctgta tatggctgcc gcggcagcga cacgttttga accgcttatt
      421 cgggatttcc accaacgcct gctgtatagc ggattaacaa aaaccggtac ggcgttgccc
      481 cgccccggct caaagggaac gattccctaa ggtgatgaag caccgggcgg atcggttccg
      541 taccatttgt accgtctgcg gcccgccgcc ttgtcctgat ttttgttaat ccgctatggt
      601 ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca acgttgagtg
      661 ttgtactatt ttgtttttaa tccactatat tctgactgcc tcaaagggca aaaataatgt
      721 gagtatgttg aggctgcctc tttttgaaat gacaaacaaa atgccgtctg aaacccgatt
      781 tccggtttca gacggcattt gatgttcaat cagttgtcaa acctgattat gccaaacctt
      841 ttgctttcaa cacttccagc atcgtcgtgc ccaactcggc agggctgcgg gtgtaggcga
      901 taccggcttt ttcaaacgcg gcgaattttt cttcggcagt acctttaccg ccggagataa
      961 tcgcaccggc gtggcccatg cgtttgcctt taggagcggt aacaccggcg atgtaaccga
     1021 caacaggttt ggttacgttg gattggatgt attcggctgc ttcttcttcc gcagtaccac
     1081 cgatttcacc aatcatgatg atggcatcgg tatctgggtc ttcttggaag agtttcagtg
     1141 cgtcgatttg gttcatacca ggaatcgggt cgccgccgat accgatacag gttgattgac
     1201 ccaagcccag tttggtggtt tgtgccacgg cttcgtaagt caatgtaccg gaacgggaaa
     1261 taataccgat gcggcccgga gtgtggatgt ggcccggcat aatgccgatt ttgcactcgc
     1321 ccggagtaat cacgcccggg cagttagggc cgaccaaacg ggtaccgttg ccgttggttt
     1381 ccaagtaacg tttggctttg agcatgtcca gagttggaac gccttcggta atcacaacga
     1441 ccaagcctac gcctgaatca acggcttcaa cgatagaatc caacacaaac ggagcgggaa
     1501 cgtaaatcac ggatgcgtca gcgccggttt ctttaacggc ttctttcatg gtgttgaata
     1561 caggcaggtc taggtgggtt tgaccgcctt tgcctggggt aacgccgccg acaactttag
     1621 tgccgtaagc cagagcttgt tcggagtgga aagtaccgtt tttaccggtg aaaccttgaa
     1681 ccaatacttt ggtgtcttta ttaatcaata cgctcattct tttctcctta ggcgtttacg
     1741 gctgcaacaa ttttttcggc tgcgtcattc agaccgtctg cagaagtcag tttcagacct
     1801 gattcgttca ggattttcgc gccgagttcg gcgttgttgc cttccaaacg aacaacgaca
     1861 ggaacgttga cgttgatttc tttaacggct gccacgatgg cttccgcaat catgtcgcaa
     1921 cgtacgatac cgccgaagat gttgatcaat acgcctttaa cggatttgtc ttccaaaatc
     1981 agtttgaacg cttcaaccac gcggtctttg gttgcgccgc cgccaacgtc caagaagttg
     2041 gcaggttggc cgcctttgag tttgatgatg tccatggtgg ccatcgccaa accggcaccg
     2101 ttaaccatac agccgatatt accttccagg gcaacatagt tcaggtcgaa ttcagaagct
     2161 ttcagttcac gttcgttttc ttgagatttg tcgcgcaatt cagcgatttt tggcaggcgg
     2221 tagagcgcgt tgctgtcgat gccgattttg ccgtccacgc aagccagtgc gccgttttcg
     2281 cgaactgcca gcgggttgac ttcaaacagg gcgaagtcgt tttcgacaaa cgctttgtac
     2341 gcgccggtca tcagtttggc gaactcgttg atttgtttgt ctttcaagcc cagttggaag
     2401 gcaacttcgc gagcttggca aggttgcagg ccgaccagcg gatcaacggt tactttgaag
     2461 attttttctg gagtttcagc agcaactttt tcgatttcca cgccgccttc ggtagaagcc
     2521 atgaatgtaa cgcggcgggt agaacggtca accactgcgc ccaagtacag ctcggtttga
     2581 acaggataca tgtcttcgca aaccaaaacg ctgttgacag gttggccgtt ggcatcggtt
     2641 tggtaagtta ccaagttggt gccaatcagg ctttcagcca cttctttggc tttttcgcgg
     2701 cttttaacga cttttacgcc gcccgcttta ccgcggccgc cggcgtgtac ctgtgctttg
     2761 acaacagcga atttgccgcc caatttgtcg taagctgcag cggcttcttc gccgttgtgc
     2821 gccaaaatac cgccttgtac gggcaaaccg tagctagcca gcagttcttt agcctgatac
     2881 tcgtgtaaat tcatggattt ctccttaagg tgagtgggtt ttaggaatcg ccctgcggcc
     2941 aatcccatat tttcagacgg tcctactatt tactgaaggg caaccacatc cgcgccagca
     3001 gatacatgac tgcaccgcct cccccgagaa taaaaaagac gatgcctttt ttacgcgaat
     3061 tcggacagag cagataaacg gcaaggctga aactgataaa caacaatgcc acgcgccaaa
     3121 ccaattcttt atctttgaaa cggttgagcg tataagtttc ggtcatcatc acatacatct
     3181 gccacaatgc ggccatacac atgctgatga tgcccatcgg tataaagaaa ataccgacta
     3241 tttctttgtt cataatgaat gtaaatgctg tttgtctgac ggaattataa atcagaaaac
     3301 gaagctctcg gaacattcaa tttgccggag gccttaaggc cgtctgaaaa tttcagacgg
     3361 ccttaatgtc tatcaaccgt gcaaagcgcg tttgtcggcc gccaatgcag cttcgtgaac
     3421 cacttcggac aaggttgggt gggcatggat aatacgggcg atatcttcgc tgctggcgaa
     3481 gaattcgagc gcagtcacgc cttcggtaac caattcgctg acaaccgggc cgatcatgtg
     3541 tacgcccaag atacggtcgg ttttggcatc tgccaacact ttaaccgtac ctttggcttt
     3601 gcccattgcc aatgcacggc cgtttgcacc gaaacctgaa gtaccttttt tgtactccac
     3661 gccttcggct ttgagctgct cttcggtttt acccacccaa gcgatttcag ggtcggtgta
     3721 aatcacaaac ggtacgttgt tgaagtcgat atgcggtttt tgaccggcaa tgcgctcggc
     3781 aacggcaacg ccttcgtcgc tggctttgtg tgccaacatc gggccgcgaa ccacgtcgcc
     3841 gattgcccat acgttaggca ggttggtacg gcattcgcca tccactttga taaagccgcg
     3901 ctcgtctttt tccaagccta cggcttccgc gttcaggcct ttggtgtttg gaatacggcc
     3961 gatggcaacg atcagtttgt cgaatacttc ggttttggct tcgccggcag cagtttcgta
     4021 agcaacggaa acacctttgc cttcagactt gatgtcgccg attttcgcac ccaattcgat
     4081 gctcagacct tgctctttgg tgaagtattt gaaggcttct ttggcgattt gttggtcggc
     4141 ggcagccagg aaggtcggcg cggcttcaag aatggtaact tccgcaccca cgcggttcca
     4201 tacggaaccc atttccaaac caatcacacc ggaaccgatt acgccgagtt tggcaggtac
     4261 ttcggtcagg ttcaatgcgc cttcgttgtc caatacgttc acgttgtcga tagcgacttg
     4321 cggcaacgga cgcggtacgg aaccggttgc tacgatgacg tgtttggctt cgataacggt
     4381 tttctcgcct ttgttatcga cttcaatttg gtaagcatcg ccatttttac cggcaaagga
     4441 agcagtaccg aacaggctgg ttactttgtt tttttggaac aggaatttca cgccgccggt
     4501 cagtttggtc acgatggcat ctttgcgctc aatcattttg gccgcgtcaa attttacgtc
     4561 gccgacagtg ataccgtgtt cggcaaaatc gtgttgcgca gcgtggaaat gttcgctgga
     4621 ttgcaacagg gctttagaag ggatacagcc tacgttcaag caagtaccgc ccaatgcagg
     4681 ggcattgcct gctttgttaa cgcctgcatc gacacaggca gttttgaaac ccagttgtgc
     4741 ggcacggatg gcggcaacgt atccgcccgg acctgcacca atcactacta catcatattg
     4801 agacatcgtg tcatcctttg cttttagcag tatctcaaaa taaaaacgat acttttcgtt
     4861 tcttttttta ttttgaatga ctgcgttttg atggttttaa gaaaaagccg tctgaaagtc
     4921 catatctgcc gattcattcg aatcaaatgt ttcagacggc ctcaatcgtt cgttgagcct
     4981 attgtagcac gatactacat tacgctacat ctaaataaaa attcagacgg ccttttcatt
     5041 aacaaataaa aagccgtctg aaacgattac agatccaaca acaggcggac cgggtcttcc
     5101 aacgcgtctt taatggctac caaggtcaat acagcttcgc ggccgtcaat gatacggtgg
     5161 tcgtaagaca gagccagata catcattgga cggacaacaa cttggccgtt ttcaaccaca
     5221 gcgcgctctt tagtggcatg catacccaaa atcgcagatt gaggcgggtt gatgatcggg
     5281 gtggacatca tagaaccgaa agtaccgccg ttggtaatac tgaatgtacc gccggtcagg
     5341 tcttcgatag cgattttgcc gtctttggct tttttcgcgt aatcaacaat tgcttgttcg
     5401 atgtcggcaa tgctcatttg gtcggcatcg cgcaggattg gtacaaccaa accgcgtggg
     5461 ctgccaattg cgataccgat gtcgaagtag ccgtggtaca caatgtcttt gccgtcaaca
     5521 gaagcattca caaccgggta ttttttcagg gctgtaacag cggctttaac gaagaaggac
     5581 ataaagccca gttttacgcc atgttctttc tcgaattttt ctttgtactt cgcacgcaag
     5641 tccatgatcg gtttcatgtt gacttcgttg aatgtagtca gaatggcgtt ttcttgttga
     5701 gaagccaaga ggcgttctgc aacacgggca cgcaggcggc tcattggtac gcgttcttca
     5761 ggacgtgcgc cggcaggaag tgcaacggca ggggccacgg ctgcggcagg tttggcagcg
     5821 gcattttgta cgtcttcttt caatacgcga ccgtcacggc cggaaccttg caatacgttc
     5881 acgtcaacac cggtctcggc agccagtttt gcagcggcag gcatggcggc gttgttttgt
     5941 gcagcagcag gaacggcagc cggggcagct tcggcaggag cggctgcagg tgcttcagca
     6001 gcgacagtag cagctgtatc gatacgggcc aaaacttggt cggcaacaac ggtttcaccg
     6061 tcttgcgcta cgatttcaac caatacgccg gcttgtggag aaggtacttc caaaaccact
     6121 ttgtccgttt cgatatcgat caggatttcg tcacgggcaa ctgcttcgcc aacttttttc
     6181 ttccattcca agagtgtgcc ttcagatacg ctttcagaca gcataggtac ttttacatca
     6241 ataatcattt tgtgtctcca atggcccttt cagacggcct gttgtgtgtt tttaattctg
     6301 catatgggca ttccgcctga gcagactcag acggaatgct acttggttta taacgccaaa
     6361 gcgtcttcaa ccaattgttt caattgagca atgtgtttgc ttgagtagcc cactgcaggc
     6421 gatgcgctgc ttggacggcc ggcataagac agtttttgct cttcgctgat aacgtcttcg
     6481 atgcggtggc ggatttggta gaacgcgcct tggtttttcg gctcttcttg cgcccaaacc
     6541 acagattttg cgttcggata tttcgccagt tcggctttaa cctcgtcgta tgggaacgga
     6601 tacagctgct caacgcggac gatagcgaca tcatcttcca gtttacgttc ggctcgaccg
     6661 gcttccaagt catagtaaac ctgaccggca cacaatacca cgcgtttcac gctgtcgttg
     6721 cttgcgcgtt cggcggtatc accaataacc ggacggaaag tcgaaccttc ggtgaagttt
     6781 tccagcgggc tcattgcacc tttgaagcgc aacaggcgtt tggacatgaa aatcaccagc
     6841 ggtttgcggt atgaacccaa gacttggcgt tgcaagagat ggaacatttg cgacgcttca
     6901 gacggcatga tgacttgcat attgttctca gaacacagtt gcaaccaacg ttctacgcgt
     6961 gcagaagagt gttcagggcc ttgaccgtcg tagccgtgcg gcaggatggt ggtcagaccg
     7021 cacaagcgac cccacttggt ttcgcctgaa gacaggaatt gatcaatagt tacttgcgcg
     7081 ccgttggcga agtcaccgaa ttgagcttcc caaatggtca gcttgtcagg agcggagcag
     7141 gcaaagccgt actcgaacgc catcacggct tcttcgttca agatagagtc gataaccagg
     7201 aactcgccca agccttcgcc catgttgcga agaggaacat aagtaccgtc gtcccatttt
     7261 tcgcgttttt gatcgtgcag aacggcatga cggtgtgaga atgtaccgcg gccggagtcc
     7321 tcaccggaga tacgcacgcc atgacctttg gtcagcaggc ttgcgtatgc gagggtttcg
     7381 gccatacccc agtcgatggc ttgtttgccg gatgccatgg ctttacgcgc ttcgatcaca
     7441 cgttttgcag tcggatgcag ggcaaagcct tccggcacag cggtgaattt ctcagtaaga
     7501 cgctcaatgt cggcggcagg caaaccggtt tcgatttttt cgcgccaatc tttaccttgg
     7561 tatttgctcc agtcgatttg tgtgcgttgg aagttgctca acgttgtttg ctcaacatgt
     7621 tcgcctttgt ccaaagcatc acggtaggct tggatgtaac cgtcggcctc aacttgagtt
     7681 accacgcctt cggcaatcag ttgctcggtg tacaaagcac gcgcacccgg atgttgcgat
     7741 acttttttgt acatcatcgg ttgggtcaaa gtcggatcat cgccctcgtt gtgaccccat
     7801 ttacggtagc agacaacgtc gattacgatg tctttatgga attttttgcg gtaatccaaa
     7861 gcggcttgaa tggcaaagca aacgcgttcg ggatcatcgc cgttcacatg gataaccgga
     7921 gcggaaacca tttttgcgat atcggtacag tgtacggttg aacgggtatc gcggatatcg
     7981 gaagtggtaa agccgatttg gttgttgatg acgatatgga ccgtaccgcc tgtggtataa
     8041 ccgcgcgttt tagacaggtt gaatgttgct tggttgacac ccaaaccgat aaatgcggaa
     8101 tcgccgtgaa tcaataccgg caagacttta tcgcggccgt tttcgcccaa acgtttttgt
     8161 ttggcgcgtg cagaaccttc cactaccgga ttgacgattt ccaagtgtga cgggttgaac
     8221 gccaaagaaa cgtgcatcgg gccgtgaggg gtggcaatat cggagctgaa gcccatatgg
     8281 tatttcacgt cgccactagg cagtttgatt tcggcacggc cttcaaattc ggcaaacaaa
     8341 tcgccgggtt ttttgcccaa aatgttcacc aaaacattca gacggccacg gtgcgccata
     8401 ccgatgatga cttcttccac gccgtcctta ccggcgtttt gaatcaggta gttcaaaccg
     8461 gcaatcgcgc tttcgccgcc ttcgacaccg aaacgtttct gaccgacata tttggtatgc
     8521 agataacgtt ccaaagtctc ggcagcggtc atctctttca agatacggcg tttttgatcg
     8581 gcattgtaat gcggtgtgga caatacgctt tcaaaatagt tgcgtaccca acggcgctct
     8641 tcggtattgg gaatatagat atattccaat gcgatgtggc cgcagtaggt ttgtttgagg
     8701 ttgctgatga tttgggacaa gggcagtttg ccgcgattgg caaaatcacc ctcgcccata
     8761 ttgaatcgaa gcgccatatc ggcatctgac agaccgtgga atttcggatc gagggcttca
     8821 atatcgcgcg gagggatacg tttgagcgga tcaagttggg ccgcacccac gccttggata
     8881 cgataggcag aaatcagccg taaaacgctg acttgctttt tcagcattgc ctcatccgca
     8941 ccgcccgcaa cggcagatgc aattttcttt ttcgccaaag taacaaatga ttcgcgaatc
     9001 ggtgtgtgtg cgacatcgac agcaaccgtc cccggctgtt tgctcaaatc ggtgaaatac
     9061 tgcttccatt tttcatcaac cgcatcgggg ttttccaaaa aagcctcgta caattcctca
     9121 atgtagggtg cgttcgaacc gaacagataa gagaaattga gtttttcgtc catcatggcg
     9181 tgcgctcttt tctttaaaat ataaacaaag ggtagaagtc cgcgtcctga cggctgaact
     9241 gatgccgtct gaaaagacat tttattctac cctattttaa accggattgc ttgtcttgat
     9301 ttcatccgac tacaatcaga cggattcagg aaacaatccg gttctctgat attgcctgtt
     9361 tgacaatata ttaatgtatg ttacctctcg cctgccggca cataatcgcg gcgttccgaa
     9421 ccggtataaa gctggcgcgg gcggccgatt ttcagcgaag gatcgctaat catctcgtgc
     9481 cagtgcgaaa tccagcccac gctgcgcgac agggcgaaga tgacggtaaa catttcggtc
     9541 gggatgccca gcgcggacag gacgatgccg gaatagaaat cgacattggg atacagtttg
     9601 cgttcgataa agaacgggtc tttcagcgca atctgttcca attccatcgc cagtttgaat
     9661 ttcggactgt cttccaagcc caattccttc aaaacttcat agcaggtttc gcgcataatg
     9721 ctggcacgcg gatccatatt gcggtatacc cggtgtccga agcccatcag gcggtatttg
     9781 cgctgtttca cgccttccat gtatgcggca acattggaca catcgccgat ttcatccagc
     9841 attttcaaca cggcttcgtt cgcaccgccg tgcgaagcac cccacaggca ggcgataccg
     9901 gcagcaatac aggcaaacgg gttcgcaccc gaagagcctg ccagacggac ggttgaagtt
     9961 gaggcgtttt gctcgtggtc ggcatgcaaa ataaagatgc ggtcgagtgc gcgtgccaaa
    10021 acgggattag gtttgtagtc ttcacacggt gtggcaaaca tcatatgaag gaagttttcg
    10081 gaataagaaa gattattctt cggataattg aacggcagac cgtttgaata gcggtaacac
    10141 attgccgcaa tggtcggaat tttggaaata aggcggtaaa tcgcgatttt gcggtgttcg
    10201 ggattagtaa tgtccaagct gtcttggtag aacgcagaca gcgcgccgac cacgccgacc
    10261 atcatcgcca tcggatgcgc gtcgcggcgg aaaccccgga agaaccaagt cagctgttca
    10321 tgcaccatcg tgtggcggcg gactgtattg tcaaattctg ccttttgctc gggagtcggc
    10381 agttcgccgt aaatcaacag gtagcagact tccaaataat cggacttttc ggccagctgc
    10441 tcgatggggt atccgcgata ataaagcaag ccttgatcgc cgtcgatgta agtaatttta
    10501 gactcacagc ttgcggttga aacaaatccg gggtcgaagg caaacaaacc tgtatttttt
    10561 gtcagccccc gaatgtcaac cacatcatgc ccgatgctgg cttccaatac cggcagctcc
    10621 aaacctgccc gacccggtac gttgagtttg attgatttgg acatattatc gctcctttgc
    10681 aagttataac ccacgttact gcatccgcag cacgttcttt gctttcagac ggcctttgca
    10741 ttttgaaatc tgccctcaga caaatttcag atgcctcatg cccgtctgat tttttcaagc
    10801 atcgggataa ggtgcccttt gtccgtttcc gaatgcccgt gaatcaaggc aagcaattct
    10861 tgatcttgaa attcaaggat ttcggaaaac tcggacagct ctttatcgct caaatgctcg
    10921 aattcttttt ccataaacct gccgaagatt aaatctagtt ccaacaatcc ccggcgggtt
    10981 tgaaaacgga ttttccgttt ggcaatgtcg tcaaaaacca tcatttctta aacaacccgt
    11041 ttcaacataa tctctttaat cttaccgatg gcccgggtcg gattcaagtg tttgggacat
    11101 acgtccacgc agttcataat ggtgtggcag cggaacaaac ggtacggatc gttcaaatta
    11161 tccaaacgct cattagtgat ggtatctcgg ctgtccgcaa tgaagcggta ggcgttcagc
    11221 aagccggacg gaccgacgaa tttgtcggga ttccaccaga atgacggaca ggcggtcgaa
    11281 cagcaggcgc acaaaataca ttcgtacaaa ccatccaact ctttgcgctc ttcctgagtt
    11341 tgcagacgct ctttgtccgc atcaatcgga ttgtcgttaa caacataagg tttgacggaa
    11401 tggtattgtt tgaagaactg ggtcatatcc acaatcaggt cgcggatgac gggcagaccg
    11461 ggcagcgggc ggattttgac aggctgtttc aagctgcgta aatcggtcag acacgccaag
    11521 ccgtttttgc cgttgatgtt cataccgtcc gatccgcaaa tcccttcgcg gcaggagcgg
    11581 cggaaagaca aggtatcgtc ttgtgctttc aggcgtacca aagcgtccaa aagtttcacg
    11641 tcggtcggtt ccaattccaa ctcgtaacgc tgcatataag gcttggcatc aacgtccggg
    11701 ttgtaacggt aaatttcaaa actcattttt tccatgagaa gtgttccttt tctcatcaac
    11761 tgcccaatga ttttgtatta taaattcaag cagttcaatg tgggtatggt taggtttcag
    11821 acgacctttg aggcgaagac cgtctgaaaa cgcatcaata aacgcgtttg gccggtttga
    11881 tgtattccac gctcaaaggc ttggtgtgta ccggtttgta agacaaggta ttggtgtccg
    11941 aatgatacag ggtgtgtttc atccaatttt cgtcatcgcg ctcaggatgg tcgtctgaag
    12001 cgtgcgcacc gcgtgattct ttacgtgctt cggcagacac caaagtcgct ttcgccactt
    12061 caatcaggtt atccaattcc aaagcttcga tacgcgcggt attccacact ttgctcttgt
    12121 ctttgatttc ggtacgtttc acacgctcgg caatcgccat gatttcttga acgcctttgc
    12181 tcagaatctc atcggtacgg aacacgccgg cgtgcagttg tacggagcgt tgcagttcgc
    12241 gacgcaatgc atcaacgttt tcaccatcgg tttgactgtc caaacgctcg atacgttggc
    12301 gggtcaactc accggcatta gcaggcagag gtttccaatc gctttgctct ttgatgaatt
    12361 taatcatgct gtcaccggca gctttgccga acaccaccaa gtccagcagg gagttcgtac
    12421 ccaaacggtt cgcaccgtgt acggaagcac aggcgcactc acctgcggca tacaggcctt
    12481 ttacaggtac ttcgtactcg tcgccttgcg gaacaacaac ttcaccgtga taattggtcg
    12541 gaatgccgcc catcatatag tgggtagtcg gcacaaccgg aatcgggtct ttaatcggat
    12601 cgataccggc aaactgaatg gaaatctcgc ggatgcccgg cagtttttcc ataatttttt
    12661 ctgcaccgat atggtcgatt ttcagtaaga cgtggtcttt gtttttacca cagccgcgac
    12721 cttcatagat ttccatcgcc atcgcgcgtg aaaccacgtc gcgagaagcc aagtctttta
    12781 cggtcggcgc atagcgttcc ataaagcgtt cgccgtcggc gttcaacaga ataccgccct
    12841 cgccgcgtac gccttcggta atcaacacgc ccgcacccgc cacgccggtc gggtggaatt
    12901 gccagaattc catatcttcc aacggaatgc ccgcacgggc gcaaatgccc aaaccgtcac
    12961 cggtattcat ataagcattg gtagaagaag cataaatacg accgccgcca ccggtagcaa
    13021 acatcacggc cttggcgtgg aaaatataaa cttcgcccgt ttccatttcc atggcggtta
    13081 cgccgacgac atcgccgttt tcatcacgaa tcaaatcttg cgccgtccat tccacaaaga
    13141 attgtgtatt ggcacggacg ttttgttggt acaaagtatg caacatcgca tgaccggtac
    13201 ggtcggcaac cgcacatgca cgttctaccg cacgtttacc atgttcggca gtatgtccgc
    13261 cgaaaggacg ctgataaatt ttgccgcttt caacgcggtc aaaaggcata cccatgtgtt
    13321 ccaactcaat caccgcttca ggcgcagcgc gacacataaa ctcaatcgca tcttggtcac
    13381 ccagccagtc ggaacctttc acggtatcgt acatgtgcca gtcccaacgg tcctcctgca
    13441 cattacccag agaggcggaa ataccgccct gcgccgctac ggtatgcgag cgggtcggga
    13501 acactttaga caaaacggca caattcaaac cggatttgga taattggagg gctgcacgta
    13561 aacctgcacc gccaccgccg acaatcacgg catcaaactt gcgaacagga aaacccatac
    13621 ttacccccaa atcactttaa ctgaatacac gaggcagccg accagccaga caatggtggc
    13681 aacctgcaaa aacaaacgca cgccgaaggg tttgatatag tccatccaca aatcgcggat
    13741 acccacccaa gcgtgcaaga atacggcgat aaagctcact tgggtaaata cttttaccca
    13801 agcttgacta aaaaatgcct gccatgccgg atattcttta ggcagggcaa atagaaccac
    13861 taaaagtgca acggtataaa tcaacataat aaccgcagtc gcacgctgca ttacccaatc
    13921 gcgcaaaccg taatgggcac cggtcaattt acgttctacc ataacaacgc tcccaaaacg
    13981 acagtcaaaa ccaatgcaga agcaaatacg gctttagcgg tattgcgcgc agtattcagc
    14041 tcaaggcctt tgtgcgcatc caaaaataaa aagcggatac cggcgagaga atggtgcaga
    14101 taagcccaca atataccgat taaaaccagc ttgaccaaag gatgggaaac aatggcacgg
    14161 taagtttcaa atgcagactc ttgactcagg gtaccggaca gaaaatacag caggaaaggc
    14221 agcataataa acagcccgac cccgctaatg cggtgaagga tggaaactat cccgggtatc
    14281 ggcagacgga tgttcggcaa atccagataa acagggcgca gtttgacaga catagtcaat
    14341 tcctctgtag ttatatatcg actcaaaaac cgtaaaccca ttgctacact gggttacatc
    14401 aacgttaatt tacctgtttt atcccatttt gaacagtgta ttttaaaaac aaactgatag
    14461 attttgacaa acacgcgtgg aaacagcaga ttaaaagaaa gatttttagt tttgccgctc
    14521 ccttcccgac acggtatcag catccgaccc ccaaccccat ccccaaaaat aaaatgccgt
    14581 ctgaaaaaaa tttcaggcgg cattgttttg ccatatcggt caggctttca atttcgcatc
    14641 ggcatcgcgc aacgcggtac gcagaccttc ctcgataaca ggatggtaga acggcatatc
    14701 cagcatttgc ggaacggtca tcttcatttg atgcgcccat gccaacaagt gagccaaatg
    14761 ttcggcggca gggcctacga tttccgcgcc gataaagcgg ccggtggctt tttcggcata
    14821 caggcgcata tggcctttgt ttaccagcat cacgcggctg cgaccttgat ttttgaacga
    14881 tacttcgccg atgacaaatt cgtcggcttg gtattgcgcg gcaacctgcg cgtatttcaa
    14941 accgacaaag ccgatttgcg gactggtaaa caccacgcca atggtgctgc ggcgcaaacc
    15001 gctgccgata ttcgggtagc ggcccgcgtt atcgccggca atcttacctt ggtcggcggc
    15061 ttcatgcagc aggggcagtt ggttggacgc gtcgcccgca ataaagatat gcggaatgct
    15121 ggtctgcatg gtcagcggat cggcaacggg tacgccgcgc gcgtctttgt cgatattgat
    15181 gttttccaaa ccgatattgt caacgttcgg acggcgacct acggctgcca acatatattc
    15241 ggcaacaaat acgccttttt cgccatcctg ctcccaatgg acttctacat tgccgtctgc
    15301 gtcgagtttg acctcggttt tagcatccag atgcagtttc aattcttctc cgaacacggc
    15361 tttcgcctcg tctgaaacaa cggggtcgga aatgccgccg atgattccgc ccaaaccgaa
    15421 aatttcaact ttcacaccca aacggtgcaa tgcctgaccc agttccaaac cgataacgcc
    15481 cggcccgaac acggcaacgc gcttaggcag cgtatcccac gagaaaacgt cgtcgttgat
    15541 aatcaaacgg tcgcccaaag actgccactg cggcaggatg acgggacgcg aaccggtagc
    15601 aatcacgaaa ctttttgccg caatttggat atggtcgtcg atttggacgg tatgctcgtc
    15661 gatgaattta gccgagccca tgatgcgctt gtcggcaggc cattcttcca catcggtaac
    15721 gacaaagccg acaaaacggt cgcgctcgga tttgacgcgc cgcataacct cttctccgtt
    15781 gacgacgatg ctgtctttgt ccaaatgcac gccgaagggg tcggtatgca atgcgtggtg
    15841 acgcgcttct gcggcggcaa tcaagagttt ggaaggcata cagcccacgc gcgcgcaggt
    15901 tgtgccgaac acattgtttt caatcaggta aacattatcc gaatgtaaac gggcattgcg
    15961 aaacgcaccc ataccggcag taccgccgcc gattacgacg atatccgctt gaattttttt
    16021 cataatcttt gtccttccgt tttcgtctga aacattgccg acacagaaca tatcggtttc
    16081 agacagcatt taaatatctt cccaatcaaa aacattattc agataaataa aaacaaaatt
    16141 tcgattgagt gcgacaatat ccaaatcttc ttgtaaatat agagttattc ggagcggcag
    16201 tatagaaccg cccgaataac tctatccggt atatcagaca gtttaatcaa actgccttaa
    16261 gcaaacagct atcagttttt agccaagtaa gcttccaaat cttcgctgcc gccgatgtat
    16321 ttgccgccga tgaagacttg aggggcagtc atcttgccgg taatagcgcg aacggaagta
    16381 acggttgcat ctttgcccaa tacgatttct tcgtaagaca aacctttgtc ttgcaaagct
    16441 tgtttgactt tggcacagaa ttggcaacct ggtttagtga aaattgccac agactcttga
    16501 gccttccaat cgggagcaac gaatttcagc atagtatctg catcggatac tttgaaagga
    16561 tcgcccggtt cttcaggttc gatgaacatt ttttcaacca cgccgtcgtt aaccagcatg
    16621 gagtaacgcc aagagcgttt gccgaagccc aagtcttctt taccgaccag catacccata
    16681 ccttcggtaa attcgccgtt gccgtcagga atcatgtaga tgttgtctga ttcttcttcg
    16741 gcagcccaag cgttcattac gaaagtatcg tttacagata cgcagcagat tgcgtcaacg
    16801 ccgttttctt tgaacgcgcc gaataattcg ttgtaacgcg gcaggtgtga agaagaacaa
    16861 gtcggggtaa atgcaccggg cagggagaat acgactactt ttttgccttt gaacaaatca
    16921 tcggtagaca catctttcca agtgtcgccg acgcgggtgc ggaatactac ggaaggtact
    16981 ttttgaccgg tacgatcttg caaagccatt ttgagctcct taatagattg gtttgagaaa
    17041 taattgtgaa tcgccgcgca ttctacccca tgcaaaatga tttgtataat ttatagttta
    17101 aataatataa attatttaaa actatcaaat acaactccga tttgcctgtt ccccacatat
    17161 aaagccgaat tagagaaaaa caagctgcaa tgaaaagatt ttccggcaac tgctcccctg
    17221 cgccgcaact caggacaaaa tgccgtctga aaggctttca gacggcattt gttcatacgg
    17281 tctcggctta tttcgccaat tcggcacgca gtttgcgggt tacgttcatc attacttgaa
    17341 gctgttccag agtttctttc cagccgcgtg ttttcaggcc gcagtccggg ttaacccaca
    17401 gacgctcaac cggaacaacc tcaatggctt tgcgcaacag gtgttccact tcggcttctg
    17461 tcggtacgcg cgggctgtgg atgtcgtaaa cgcccgggcc gatgtcgttc gggtatttga
    17521 attcgccgaa cgcagccaag agttccatgt cggaacgtga agtctcaatg gtgatcacgt
    17581 ccgcatccat tgcagcaatc gcaggcagga tgtcgttgaa ctcagagtag cacatatgag
    17641 tgtggatttg agtgctgtct tcgcaacctg cagaggatag gcggaaggat tcgcccgccc
    17701 agttcaggta ggcatcccaa tcggcacgtt tcaaaggaag accttcgcgg atggcaggtt
    17761 cgtcaatttg gatgacttta atgccggctt tttccagatc caatacttcg tcgttcagag
    17821 ccagtgcgat ttgtttgcac acggtagcgc gcggaatatc gttgcggacg aaagaccatt
    17881 gcagaatggt tacaggaccg gtcaacatac ctttcatcgg gcgtttggtc aggttttgtg
    17941 cgtaagtaga ccaagcgaca gtcatagctt caggacggct tacgtcaccg aagatgatag
    18001 gtggtttaac gcagcgtgag ccgtagcttt gcacccagcc gtattgggtg aatgcaaaac
    18061 cgctcaacag ttcgccgaag tattcgacca tgtcgttacg ctcggcttcg ccgtgcacca
    18121 gtacgtccaa gtccagtttt tcttgctctt caaccaccaa ggcgatttct tttttcattg
    18181 cggcttcgta atcggaggca gacagttcgc cttttttgaa ggctgcgcgt gcttggcgga
    18241 tttcggtagt ttgcgggaaa gaaccaatgt tggtcgtcgg cagcagaggc aggttcaacc
    18301 atgcttgttg cgctttgata cggtcggcaa acggtgattt gcgttggtct gcgttgacag
    18361 gcaggtcggc caagcgtttg gcaacgtctg cacggtggat ttcactgctg ttggcacggg
    18421 agtcggcagc agcttggctg gcggccagtt cttcggcaac agaatcacgg ccttcattca
    18481 atgcggcttt cagaacacgc aattcttggg ttttttgcag agtgaatgcc aaccaagagt
    18541 acaggtcggg tttgttggct ttcagttttt cttcgactga caagtcaaac ggagtgtgca
    18601 gcagcgagca agagctggaa atccacaaac gctcgcccag tttggcttgc agaggcccga
    18661 cggtttccaa aactttgttc aggttggcgc gccaaatgtt gcggccgtca atcacgccgg
    18721 cagacaggac tttgtcgtag ccggcgaacg cgtccagttg ctcgggtgcg cgtaccaagt
    18781 cgatgtgcag gccgtcaaca ggcagggatt tcaacaatgc ggcgtgttcg gcaacagaac
    18841 cgaagtaagt gcccaacagg attttggcgc ttactttgtt caaagtagcg taaacgtctt
    18901 tgtatgcttc tacccattct ttaggcagat cgacagccaa agcaggctcg tcgatttgaa
    18961 tccactcggc accggcttca accaaagcag tcaggatttc aacgtaaaca ggcaacagtt
    19021 taggcaacag gctcagacgg tcgaattcaa cggagccttt ttctttaccc acccacagga
    19081 aagtcaaagg accgacaacg gtcggtttgg ctttcaagcc caaagtttgg gcttcttgca
    19141 gttgttgaac gtagtgtttg gcgttggctt tgaattcggt atcggcatgg aattcaggca
    19201 ccaggtagtg gtagttggtg tcgaaccatt tggtcatttc gatggcgaat tggtctttgt
    19261 tgccgcgcgc cagttggaag aattgttcca aaggcaggtt ttggctatcg aagccgaaac
    19321 gggcgggaat cgcgccggtg gcaacttgca ggtcgaggat gtgatcgtag aaagtgaaat
    19381 cacctacagc aacgtaatca gcgttggcag cagcttggtg tttccagttt ttctcgcgca
    19441 ggtctttggc aacgtcgagc agctcttgtt cactgatctc tttgcgccag tatttttctt
    19501 gggcgaattt caattcacgg aaggcgccga cacgcgggaa gcctgagaaa tgtaatgttg
    19561 tcatgttgaa ctctccagtt ggattttgta aggaccggtt caatccgaat cggccggtat
    19621 tgtaaatcag aatggctcgg atagggcggc gttttgcttt tcagacggcc tcggatgctg
    19681 tctgaagccg ttttcggctt taagggcgcg cgcctaaaat atggcagatg gcgtaagtca
    19741 gctcgctgcg gttgagcgtg tagaagtgga aatctttcac gccttcgcgg gacaggactt
    19801 taaccatatc gatggcgata ctggcggcga cgaggttgcg cgtgccttgg tcgtcgtcca
    19861 agccttcata catttgcgac agccatttcg ggattttgac gttggttact tgtgccattt
    19921 tgccgagttg cttgaagttg gtaacaggca ggatgccggg aacgatttcg acgtcgatgc
    19981 ccaacatcac gcagcggtcg cggaagcgca ggtagcgttc cacatcgaag aagaattggg
    20041 tgatgacgtg gttcgcgccc gcatcgattt tgcgcttcag attaatcagg tcggcttgtg
    20101 cggatttcgc ttcgggatgt acttcggggt atgctgctac agagatgtcg aagtcggcga
    20161 cggagcgtaa tagcttaacc aagtcttcgg cgtaaaacgg ttttttctca taaccggccg
    20221 gctcgtcgcc acgcagggca acgatgcggc ggataccgct gtcccaatag tctttggcga
    20281 tttggcgcaa ttcgtcgtgg gatgcatcga tgccggtcag gtgcggagct gcttccaagc
    20341 cggtttcctg tttgatgcgt ttgacgatgc cgtgcgtgcg gtcgcgttcg ccggagtttg
    20401 cgccgtaagt tacggatacg aacttgggat gcagggtttg cagacggtgg atggaatccc
    20461 acagcatcgt ttccatttgt tcgtttttcg gcgggaaaaa ttcaaacgaa acgttgatgt
    20521 tgcctttcaa atcggaaagg ctgttgttta acgcattgat ttcttttgca taatttatct
    20581 gcataccttc gttatctttt acatgatgtt tcaggcggta tgataaggtt gcaccgaata
    20641 tgagtcaatt tcaaaatttt caggtttatg atgaaaattt ttaatattta gcaaaaacta
    20701 tacgcttttc ttgtacaatc cttctaaatt atgataaagt ttgttatgtt ataacacttc
    20761 aatcaaggca atatatgaaa ccgaatattc atcctgacaa ttatcgaacc gtcctttttt
    20821 tcgacagcag tgccaatgaa ggctggctga ttcgttcttg cgccggaacg cacggaaaaa
    20881 caatggtttg gacagacggc aaagaatatc cgctcttttc cttagatact tcctcttcct
    20941 cacatcccgt ctataccggc aaacagcgta acgtcaatac cgaaggccgt gccagcaagt
    21001 tcaaccaacg tttccaatcc gtcatgtcat cgtttagaaa ggacaaataa tgcaggttct
    21061 gtcttcgctc aaaaccgcca aacaacgcca ccgcgactgc caaatcgtca gacgcagggg
    21121 caaagtgtac gtcatctgca aaagcaatcc gcgttttaaa tcgcgccaac gctgaccccc
    21181 tgcccttcct gcttcggttg taaagcccct cctaccgcaa gcccttttca aatatgccgt
    21241 ctgaaacaga aaaatgcgtt tcagacggca tttttccgtc gatacgaatc cggaatgtgt
    21301 ttgaaataca gcataaacag ataccaaaac agcgtcgcgg cggctgcacc gagcagtacc
    21361 catgcgctga tttcggcgac ttcgcgcagg acacgtttct gcctgcggct aaagaggcgg
    21421 ttgataatca gcgcggcaag tgcgaaaaag aaaaaaatac ggaaaagtct gcctatcatg
    21481 tcgggaagcg aaaagtatgg ttcgggcgcg gcggcatggg aaaggatgtt tcagcgcgtc
    21541 aggctgcttg tccgaccgtg tcaaaaagtc atgccgtttg cgatataata acgcttttcg
    21601 ccgcatttgt tggaacagga tatgagtcaa aatacctcgg tgggcattgt aacgccccaa
    21661 aaaattccgt ttgaaatgcc gctggttttg gaaaacggta aaactttgcc gcgtttcgat
    21721 ctgatgattg aaacctacgg cgagctgaat gctgaaaaaa acaatgcggt tttaatctgc
    21781 cacgcgctgt cgggcaacca tcacgttgcg ggcaggcatt cggcggagga taaatatacg
    21841 ggctggtggg acaatatggt cggtcccgga aaaccgattg atacggaacg ttttttcgtg
    21901 gtcgggttga acaatctggg cggctgcgac ggcagcagcg ggcctttgtc gatcaatcct
    21961 gaaacgggca gggaatacgg cgcggatttt ccgatggtta cggtgaagga ctgggtaaaa
    22021 tcacaagccg cgcttgccga ttatctcggc atcgaacaat gggcggcggt tgtcggcggc
    22081 agcttgggcg gcatgcaggc tttgcagtgg gcgatttcct atcccgaacg tgtgcgccac
    22141 gccttggtga ttgcgtctgc gccgaaactg tccacgcaaa atatcgcgtt taatgatgta
    22201 gcacgtcagg cgattttgac cgaccccgat ttcaatgaag gacattaccg cagccacaac
    22261 accgttcccg cgcgcggttt gcggattgcc cgtatgatgg gacacattac gtatcttgcc
    22321 gaagacggtt tgggcaaaaa attcggacgc gatttgcgtt ccaacggcta tcaatacggc
    22381 tatagcgttg aatttgaagt agaatcctat ctccgctatc aaggcgacaa attcgtcggg
    22441 cggtttgatg ctaatacata tttgctgatg accaaagctt tggactattt cgatccggcg
    22501 gcggatttcg gcaacagcct gactcgcgcc gtgcaggatg tgcaggcaaa attctttgtc
    22561 gccagcttca gcaccgactg gcgtttcgcg cccgaacgtt cgcacgaact ggtcaaggca
    22621 ctgattgccg cccaaaaatc cgtgcagtat atcgaagtca agtccgcaca cgggcacgat
    22681 gcctttttaa tggaagacga agcctatatg cgcgccgtaa cggcttatat gaacaatgtt
    22741 gacaaggatt gccgattatg aacctgcgcg atgatttgca actgatttac gaccggatac
    22801 ccgaaggcag ccgcgtctta gacttgggct gcggcgacgg cgaattgctg gcggctttgg
    22861 cggaacacaa aaaatgcagc ggctacggca tcgaaatcga caccgacaac gtgattgctg
    22921 cgatgtcgcg cggcgtaaat gttatccaag ccgatttgga agaagggttg accgcattca
    22981 acgatcaaag ttttgatgtg attgttttga gccaaaccat ccaagcgatg cagaataccg
    23041 aaaaaatcct gcgctgcctg atgcgcctcg ccaaacaggc aatcgtcagt ttcccgaatt
    23101 tcggctactg gcgcaaccgc gtccaaatcg cactcggcgg acatatgccg gtttccgaac
    23161 gtatgcccta ccattggtac gacacgccca atatccattg gtgcacgctc aaagactttg
    23221 atttgttgtg cgccaaaaac aatatccgcg tcctcgaacg cgccgtgatg acgggcaaca
    23281 gacaggtcaa acatttcccc aaccttttgg gcagccttgc gttttaccgc gtcgggtaag
    23341 acaggttgaa aaccttgtcg gcaataacgg gtttctcggt tctattaaca acagtccgtc
    23401 tgaaaaatga aacggcaggt tttcagacgg ccttttcaat attggaaaca atagaatgaa
    23461 aaacaaaacg tcatcacttc ccttatggct tgccgcaatc atgctggccg cgcgttcccc
    23521 gagcaaagaa gataaaacga aagaaaacgg cgcatccgcc gcttcgtctt ccgcgtccca
    23581 atccgatttg caaccggccg catccgcccc tgataacgtc aagcaggcag aaagcgcgcc
    23641 actgtgaaat tgcaccggcc tgcaccccgc cgccggcatt ggcgatctca tacagcaaat
    23701 cgccgaacac atcgactcgg actgtctgtt tgccctttcc cataacgaac tggaaacccg
    23761 tttcggctta cccggcggcg gctatgacaa catacagcgg ctgctgtttc ccgacatccg
    23821 ccctgaagat cccgactacc atcagaaaat catacaggca attgaagact tgcgttacgg
    23881 aacgcgcacc atcagccggc aggcacaaga tgccatgatg gaacaggaac gccgcctccg
    23941 agaagcgacg ctgctgctga cacaaggcag tcaagaaacc cgcggacaag gcgaggaacc
    24001 gaaacgcgca cgttattttg aagtttcggc aacatctgcc tatttgaacc ggcacaacaa
    24061 cggactcggc ggcaatttcc aatacatcgg ccaattgccc ggctatctga aaatgcacgg
    24121 agaaatgctt gaaaaccaat cactcttccg gctgtccaac cgtgaacgca atcccgacaa
    24181 accgttttta gacatccatt ttgacgaaaa tggcaaaatc acgcgtattg tcgtttacga
    24241 aaaaaatatc tatagtggat taaatttaaa tcaggacaag gcgacgaagc cgaagacagt
    24301 acagatagta cggcaaggcg aggcaacgct gtactggttt aaatttaatc cactatactt
    24361 caatccaaac acggggcgaa tataaaaaaa tgccgtctga aagcctttca gacggcattt
    24421 ttcaagtagg atatcaggcg cgtttgcgga attcgccggt acgggtatcg atttcgactt
    24481 tgtcgccgtt ttcgatgtaa gacatcactt ggatttcggt gccgccgacc agacgcgcgg
    24541 ttttcatcac tttgccggaa gtatcgcctt tgacggcagg ctcggtgtag tcgacttcgc
    24601 gcacgatgat ggtgggcagt tcgacagaaa tgggattgcc ttcatagaag gtaacttcgc
    24661 attggtcttc cataccgtca acgatgaatt tcaacgcgtc gccgatgttg tcggcttcga
    24721 tttcgtattg gttgaattct tcgtccataa agacgtacat cgggtcggca aaatagctgt
    24781 acgtacagtt tttgcgggac aggatgacca cgtcgaattt gtcgtcggct ttgtaaatgg
    24841 tttcggaagc agcgccggtc agcaggtttt tcagtttcat gctgactttg gcggaagagc
    24901 ggccgccttt gatgtattcg gttttttgaa cgaccatagg atcgttgccg accataaata
    24961 cattgccggc gcgcagttct tgtgctgttt tcattgtgat tttccaatca aatcggttgt
    25021 cgtaaaacgc gctattctag cgtatttttt gatgctttga aacaaaggcg gcgagttttt
    25081 cggatgcgga aggctgcccg aaaagataac ggctccaatc ctccgcgcct tgccgccagc
    25141 cgttttgatg ttgttgcagg gtttgccaac attcgaggcg ttgtgttgcg gataaagcct
    25201 ctccgccgtt gaggtcgtcc gaaaggaggc ggtgcaccga tgcggtttcg ggcgtgtaga
    25261 agccgtatgc cttatcccaa aaggcgtgga gtttgtcgag atggacattc tcgtcttgcg
    25321 ggtagatgtg ccaaaaaaag ggttttccgg caagctgggt acgcacgaaa ctgtcttcgc
    25381 cgcgtatcac ggcgcagtcg gcgaggtgca gcaatttgtc gaagtcctgt tgcggcacga
    25441 acgggatttt gacaaggcgg acggatgccg tctgaaagac accgccgtca ttttgcaggg
    25501 cgttttgcgg aataacgccg ctttgtttga ggctgtcgat aatttgcgcc cccgccagca
    25561 gtagggtcat caggctgcct gcctgttgcc acatgtccag ccactttgcc caaacatcgc
    25621 cccgatagcc gaaaagcagc cattcggggg cgtttttttc gggcagcacc agccgccggc
    25681 gcagggcttc ggtatcgaaa cggacggctt cgcggtaatc gcgttcgcgt atcaacccgc
    25741 cgcttttttc gctgaaaccc ataaaccaaa aatatttttg aacgccctcc tgcggcgaag
    25801 gcatcaggtg cagcctttca ttgctttcct ccgcgctcaa atattcccaa ttcagccaaa
    25861 gcggtttgtg tcggcggatg atgttcagca cattttcggg caggtcgcag gcaaaagttt
    25921 cgataacggc atcgggaacg ggcgcggtat caatgtctgc cgcatcggaa tgccaagtgc
    25981 ggacatgaat atcctgatga acgaagggaa catcgggcaa atcgggacaa agcgcgcgca
    26041 aggcggacac gtcgtccgtc cacaaatgca cctgccaacc gagttcgcgg tgcaaaacac
    26101 gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc gatgaccttg caaaaaatcc
    26161 aacagacagg aaaagcgtat gtattcataa ccatcgggaa aacaagctaa aacgcgccga
    26221 aatagtggaa caaatacaca accagcaaaa caatcggaat aaacagaaac atggtttgac
    26281 gtttcaaatc aaacccttgt ttaagatgat gcttgatggt ttcaaagagg acgaatttaa
    26341 acaaaaacgt cgcttcgcag gcaaggatga tgccgttgat cagatgctgg ggtttgtctt
    26401 cgggcggatt ggcggcgaaa acggcaagcg tcgccaaggg gatgaggcag acggcgagcc
    26461 agcggacaag caggtttcgg gacattgcaa ggctttcaaa aaagcaaaac gccattatcg
    26521 cctcaagaac gcaccttgtc taacggacgc gccggagatg ccgtctgaag ccttgccgcc
    26581 ggaaaacgcc gcaccaccga acctttaaat atacccggga cgggcgttcc ccgagaaatc
    26641 ggacgctgcc gccgatgcct gcggacaagg cggcatcggg cgcgggcata ttgaaagccc
    26701 gacgcatcgg tttgaacgca ttttataaag gcgtacggca aacccgcccc ttgccgtcgg
    26761 cgtgcttccg caaagcaaaa tataagggcg gcgcgctttc cgctataatc cgcatttttc
    26821 caaacctgaa acattatgcc gaccccgaaa gcctttaccc tgctcgggcc gaccgcctgc
    26881 ggcaaaaccg cgctcgccct caaaattgcc gaaacccttc cggtcgaaat catcagcctc
    26941 gattctgcac tgctctatac cggtatggac atcggcacgg cgaaaccgtc cgcctccgaa
    27001 cgcgcctttg tcccgcacca ccttatcgac atcattacgc ccgttcagac atacagtgcc
    27061 gcccgttttg tcgaagactg cacgcgtctg accggcgaaa tcacggcgcg tgggaaatgt
    27121 ccgctgattg tcggcggcac aatgatgtat ttccgcgccc tgacccaagg tttgaacgat
    27181 ttgcccgaag ccgatgcctg cctgcgtgcc gacttggacg aacaaaaaca gatgtacggt
    27241 ttggattttc tctaccgcac cctgcaaaaa gtcgaccctg aaacagcatg ccgtctgaaa
    27301 ccgaacgaca gccagcgcat cggacgcgcc cttgaagtct attacctgac cggcaggccg
    27361 atgagcgcgc acctgaacgg acagcccgaa cacaccctgc ccttcgaact ctacaccgcc
    27421 gccctgattc ccgaaaaccg cgcccgactg cacgaaaaca tcgccctgcg tttccacctg
    27481 atgcttgaac agggctttat cggcgaagta gaaaacctgc gccgccgcta tcccggcctg
    27541 accgccgact cccccgccat ccgctgcgtc ggctaccgtc aggcgtggga acacctcgac
    27601 ggtgcaaccg acagacaaac cttcatcgaa aaaggcattg ccgccacgcg ccaacttgcc
    27661 aaacgccaac tgacttggtt acgcaaaaca cctttagact gcgttgccga cccgttttca
    27721 gacggcactt caggcacgcg cctgattgag gcggcaaaac ggtttttcgg cgaataaacc
    27781 atgctggcca cgccgccgct cgcccccaaa accgtcgccg ccctgcacag gctcggtatc
    27841 cgtacgctcg aagaactgcg gcaaaacggt tcggtcaaag cctttctgct gctcaaagca
    27901 tcgggtttga ccctcacgaa aagcacgctg tggcaactcg agtcgctgct gaacggcacg
    27961 ccgccgcaag aaatgtccca agcgcataaa gactgcctgc ttgccgagtt gaaaaaccat
    28021 ccgcccgtcg ccgcctttcc gccgcaggaa gaaatgggat acttcatgcg cgaagcactg
    28081 cgccaagcag aacaatccgc cgccgacggc gaaatacccg tcggggcagt catcgtttca
    28141 gacggcaaaa tcatcacatc ggcacacaac acctgcattg ccgactgcaa tgtcagccga
    28201 cacgccgaaa tcaacgcctt ggcacaggca ggcagcgaaa tgcaaaacta ccgccttgac
    28261 ggatgcgaca tctacatcac cctcgaaccc tgcgccatgt gcgcgtccgc actgatacag
    28321 gcacgaatca ggcgcgtgat ctacggtgcg gcagaaccca aaaccggcgc ggcaggcagc
    28381 atcgtcaacc tgtttgccga caaacgcctc aatacgcaca ccgccatacg gggcggaatc
    28441 ctgcaagaag aatgccgcgc cgtattaagc cgttttttcc aaaacaaaag aaaaggttga
    28501 acccatgacc gccctactcg tcatcctcgc cctcgccctg atagccgtcg gcacggcagg
    28561 catcgtctat cccgccctgc ccggcttggc attgatgttt gccggaacat ggctgcttgc
    28621 ctatgccggc ggctatcaaa tctacggcgc aggcatcttg tggacggtcg gactcatcag
    28681 ccttggcggc atactggcgg actatatggc aggcatgttg ggggtaaaat acactggggc
    28741 aggcaaactc gccgtccgag gtgcattggc cggcagcatc atcggcatat ttttctccct
    28801 gcccgggctg atactcggcc cctttatcgg cgcggcggca ggcgaactga tcgatcggcg
    28861 caatatgctt caggcaggta aagcgggctt gggtacgctg ttggggcttg tcgtcggcac
    28921 ggcgttcaaa atcggctgcg ccgtatccat cttgcttatc ctgttggtga aatacatcgc
    28981 ctacctgttt taaccccttc agacggcata ccggcaaatt gctataatgc cgtctgaaat
    29041 ttccgacaaa gcaccattat ggcaaaccaa agacccatct acggcttcca cgccgtcaac
    29101 gcccgattgt ggcaaaaccc caaatcgatt accgaactct atatccaaga aggcaaatcc
    29161 gacgcacgca cgcgcgaagt gctggaaaag gcggcaaacg aaaacatccg cgtatatttt
    29221 gccgatgccg accgcctcaa cgccatcagc aaaggcgcgc gccatcaggg cgtggtcgga
    29281 tttatcgatg cctccaaaaa ccacgtccac ctcgaagacg tattggaaaa cctcagcgaa
    29341 ccgccgctgc tgctgatact cgacggcatc accgatccgc acaacctcgg cgcgtgcctg
    29401 cgtaccgccg acgcaatggg cgtacacgcc gtcatcgcgc cgaaagacaa aagcgcggga
    29461 ctgaacgcca ccgtcagcaa agtcgcctgc ggcgcggcgg aaaccgtccc ctacatcacc
    29521 gtaaccaacc tcgcccgcac cctgcgcgaa ttgaaagaat acggcatctg gattatcggc
    29581 accgacatag gcggcaacgc cgacctttac cattgcaatc tgcccgacag cgcggcatgg
    29641 gtgatgggca acgaaggcga cggtatgcgc cgcctcacgc gcgaacattg cgacatgctg
    29701 gtgtccatac ccatgttcgg cacggtcgaa agcatgaacg tctccgtgtc cgcaggaatg
    29761 gtattaagcg aaacccgccg gcaacgcgta ttgaaaaaca aaaaagcgta agcctcaaaa
    29821 acaatgccgt ctgaaacagg gattccgttt cagacggcat tcgttttcaa aatcagtgtt
    29881 ccaaatggtc ggtatcaaac ttgaccgccg cctcggtttt atcttccggc aagaccaaat
    29941 tcagcaagac tgccgtaatg ccgccgccgg aaatagagtt ttggaacaag acgggcaggt
    30001 ttttaaacac ttccggctca aaccccacgc ccaagcccaa accgaccaac gttgccgcaa
    30061 tcaccgcctc gcgcctgcgg atgccgtggc cgaccaaaat ccgcacgccc gcaatcgcaa
    30121 tcaggccgaa catcaaaacc atcgcgccgc ccaacagcgg actcggaatc gtcgtaaacg
    30181 cgcgtccgac aacggggaac agacccaaca gcaccaaaat cacggcaata tatttgccca
    30241 cgtggcgcga agccactccg gtcatctgaa tcacgccgtt gttttgcgca aacgtcgtca
    30301 gcggcagcga acccaaagcc gtcgcaatca ccgataccaa gccgtcagcc aacacgccgc
    30361 cgcgcaggcg tttggtgtat tcttcgcctt caatcggctg gtcggacacc attgccgttg
    30421 ccgtcaaatc gccgaccgcc tcaaacacgc ccaacaagaa aatcgcaccc gccgcaataa
    30481 atgcgtgcca gtcaaaagca aaaccatatt taaacggtac gggcagcgta accggcggca
    30541 ggttttgcag tgcggaaaaa tccaccttgc ccaaaaacag cgcgacaatg tagccggcaa
    30601 tcagaccgac cgcaatgccg ctcatccgca gcagcggatt tttcatgcag ttgaacacca
    30661 gcacaatcag cagcaccagc gaagccagcc ccaagttttc catcgagccg aacgtgccgt
    30721 ccgccttcgc gccgaagccg ccgccgaaat cggcaatacc gacgtgtacc aaactcagac
    30781 caatcagcat cacaaccacg ccgctgaccg tcggcgtaat cacttttttc aaatacggca
    30841 gaagccacgc cgagaaacac accaaaaacg caccggcaaa cgatacgccc aagagcgtcg
    30901 aaaccatcgc cccctcactc aaaccgccct ctttcatccc cgcgccgagc gcaatcatga
    30961 cggtaacgct ggatggacag catccccgaa ccgaccggcc cgaagcggtt gacctgcaaa
    31021 taagtgccga cacccgacgc aaccatcgcc atcgacacga gatacgccgt catttccacc
    31081 ggtagttcca gcgcgccgcc cacaatcagc gcgggcgtaa tcatcggcac gaaaatcgcc
    31141 aaaagatggg taaccgcgct caagagcgca ttaccgaacg gcggcctgtc ttccaaaccg
    31201 tacaccaaat caggcgaatc cgcctgtttt ttcattgttt cagccattgg agtctttcta
    31261 tccgttgcca aacgttaaaa aattttaaga acgaaattgt aacgaatcaa agattcctta
    31321 tcaattcaaa tcctcatgtg caaaaacacc accggttgtc cgaattcaaa tttctttgaa
    31381 tttccgcacc ggacagtcct tcatttccag agagccttca tgcgcttaac acattttaat
    31441 cctcaacggc gcatccactg cttaaaccca ataatttcat gattataaaa aaaatattat
    31501 ttattaagat atgatgggac ttaagccttg gaaccaaccg tgcggccgat atgtttcaca
    31561 caaacaacaa agtaagctac aatccgaagc ataatcgacc tcactcaatg aataaaaggt
    31621 ttccgtatgt tacaaggtag cttggttgcc ctgattaccc cgatgaatca agacggcagc
    31681 atccattacg accaactccg ccaattaatc gactggcaca tcgaaaacgg cacggacggc
    31741 atcgtcgctg ccggcacgac aggcgaatcc gccaccctct ccgtcgaaga acatttaagc
    31801 gtaatcgaag aaacggtcaa acacgttgcc aaacgcgtcc ccgtcatcgc cggcacaggg
    31861 gcgaacaaca ccgtcgaagc catcgccctt tcccaagccg ccgaaaaagc cggcgcggac
    31921 tacaccctct ccgtcgtccc ctattacaac aaaccttccc aagaaggcat gtaccgccat
    31981 ttcaaagccg ttgccgaagc cgccgcggtt ccgatgatac tctacaacgt gcccggcaga
    32041 accgtcgtca gcatgaacaa cgaaaccatc ctgcgccttg ccgaaatccc caacatcgtc
    32101 ggcgttaaag aagcaagcgg caacgtcggc agcaacatcg aactcatcaa ccgcgcgccc
    32161 gaaggcttcg tcgtcctttc cggcgacgac cacaccgccc tgccgttcat gctctgcggc
    32221 ggacacggcg ttattaccgt agccgccaac gccgcgccca agctctttgc cgatatgtgc
    32281 cgtgccgcgc tgcaaggcaa cattgccctt gcacgcgaac tcaacgaccg gctgattccg
    32341 atttacgaca ccatgttctg cgaacccagt cccgccgcac cgaaatgggc ggtatccgcc
    32401 ttgggcagat gcggaccgca cgtccgcctg ccgctcgttc cgctgacgga aggcggacag
    32461 gcaaaagtcc gcgccgccct gaaagcctca ggacaacttt aaatatagcc atgccgtccg
    32521 aaccgttcgg acggcataac atgacaaaca ctttaatatc catcacacag gatgacacga
    32581 tgacccatat caaacccgtc attgccgcgc tcgcactcat cgggcttgcc gcctgctccg
    32641 gcagcaaaac cgaacagccc aagctcgact accaaagccg gtcgcaccgc ctgatcaaac
    32701 tcgaagtccc gcctgatttg aacaaccccg accaaggcaa cctctaccgc ctgcctgccg
    32761 gttcgggagc cgtccgcgcc agcgatttgg aaaaacgccg cacacccgcc gtccaacagc
    32821 cagccgatgc cgaagtattg aaaagcgtca aaggcgtccg cctcgagcgc gacggcagcc
    32881 aacgctggct tgtcgttgac ggcaaatccc ccgccgaaat ctggccgctt ctgaaagcct
    32941 tttggcagga aaacggcttc gacatcgaat ccgaagaacc cgccatcgga caaatggaaa
    33001 ccgagtgggc ggaaaaccgt gccaaaatcc cccaagacag cttgcgccgc ctattcgaca
    33061 cagtcggttt gggcggcatc tactccaccg gcgagcgcga caaattcatc gtccgtatcg
    33121 aacagggcaa aaacggcgtt tccgacatct tcttcgccca caaagcgatg aaagaagtgt
    33181 atggcgacaa aaacaaagac acgaccatgt ggcagccttc cgcttccgac cccaaccttg
    33241 aggccgcttt cctgacgcgc tttatgcaat atttgggcgt tgacggacgg caggcggaaa
    33301 acgcattggc aaaaaaaccg acccttcccg ccgccaacga aatggcgcgt atcgaaggca
    33361 aaagcctgat tgtctttggc gactacggca gaaactggcg gcgcaccggc cttgccctcg
    33421 accgcatcgg actgaccgtc gtcggtcaaa acaccgaacg ccacgccttc ctggttcaaa
    33481 aagccccgaa cgaaagcaat gcagttaccg aacaaaaacc ggggctgttc aaacgcctac
    33541 tgggcaaagg caaagcggag aaacctgccg aacagccgga actgattgtc tatgccgagc
    33601 ctgtcgccga cggttcgcgc atcgtcctgc tcaacaaaga cggcagcgca tatgccggca
    33661 aagacgcatc cgcactgtta ggcaaactcc attccgaact gcgttaaacc gttcaaaaca
    33721 aaagcagctt ccaaacagaa gctgcttttt tgtgcggaac tttttacaac aaacggggca
    33781 aaatatcctc aaccgcctga accaacgcat ccgccaaagg cggatcgaac gcacaatgcc
    33841 ccgcctggac caccctcaat tccgcttcgg gaaaggcttt cgacagctcc cacgcactct
    33901 gcataggcgt acacaaatca taccgcccct gcacgataac ggtcgggata tgccgtattt
    33961 tgccgatatt gttcaaaatc gccttatcac cctgcaacca accgccgttg acaaaataat
    34021 ggttttccaa acgcgcgatt gccagtgagg cataagcatc ttcatccact ccctccggct
    34081 cgaaacgaat cagatagctt tcccaatccg cccaagcctt cgcggcagac aggcacactt
    34141 cttcatcttg atgaaacagc aacccgtgat acgcctcaat cagccggttc cgccgatttt
    34201 cagcaatcgg cgcgacaaat ttttgccatt gttccggata aatccggctc acgccgcccg
    34261 cctcgttcag ccacgccgtt tcagacggcc tgcacaaaaa tatcccgcgc aacaccaatc
    34321 cctttacccg ttcgggatgg gtttgggcat aagccagcga caaagtgctg ccccacgaac
    34381 cgccgaacac cagccatttc ccgataccca gcatttcacg gactttttca atatccgcca
    34441 ccaaatccca agtcgtattg tcttccgcac aagcatacgg gtgcgaacgt ccgcaaccgc
    34501 gctggtcgat gatgacgatg cggaacacat caggattgaa aaaaccccga cattcaggcg
    34561 aagcccccgc gcccggcccg ccgtgtaaaa aaatgaccgg cacaccgtcg ggattgcccg
    34621 actcttccca ataaatttga tgaatttcag acacctgcaa gtatccgcta tgaaaaggct
    34681 gttttatttc atacatcatc ttctcccata ttgcttctga caatccgaac atcccgacac
    34741 gcgacatccc gcacgaaaca caagccgccc cgaaaggcaa atgccgaaaa atcgcgataa
    34801 aaaccatcaa tcggaaaata ttgcataaac aggcgaaaat atatcattga aaagaaaacc
    34861 catacaatgt ctgacagaaa caagccgctt tgccattgtt tcctaacagt taaaccccgc
    34921 ccttcaaggc ggcggggcag ggcttgattc aaaatggcgc aagcccctgc cctcaaatcc
    34981 aacacgcagg attaaaccat aatagcggct ttcttattat ttcttattga aacaccgccc
    35041 ggaacccgat ataatccgcc tttgaagcat cagtgaaaat ctttttttta accggt
//
LOCUS       DAWTCI010000013        33956 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWTCI010000013 DAWTCI010000000
VERSION     DAWTCI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33956
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.013"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(22..1332)
                     /locus_tag="WM481_000653"
     CDS             complement(22..1332)
                     /locus_tag="WM481_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFA9080027.1"
                     /translation="MDISKQTLLDRVFNLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFIGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFVMVVVLGYFRVQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPGIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPVFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTGDVPPMVWVVAVLWALKFWYLG"
     gene            complement(1468..2646)
                     /gene="ftsZ"
                     /locus_tag="WM481_000654"
     CDS             complement(1468..2646)
                     /gene="ftsZ"
                     /locus_tag="WM481_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFA9080028.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRSVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFSYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDPTPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2765..4009)
                     /gene="ftsA"
                     /locus_tag="WM481_000655"
     CDS             complement(2765..4009)
                     /gene="ftsA"
                     /locus_tag="WM481_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFA9080029.1"
                     /translation="MEQQKRYISVLDIGTSKVLALIGEVQDDDKINIIGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIELCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGT
                     VQEREGGGGLLARLKRWIENNL"
     gene            complement(4095..4823)
                     /locus_tag="WM481_000656"
     CDS             complement(4095..4823)
                     /locus_tag="WM481_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFA9080030.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWNVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRHAPDGLPEKESEE"
     gene            complement(4813..5727)
                     /locus_tag="WM481_000657"
     CDS             complement(4813..5727)
                     /locus_tag="WM481_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFA9080031.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKERGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLYDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVTGVGFADLCIEILKAAHVG"
     gene            complement(5839..7248)
                     /locus_tag="WM481_000658"
     CDS             complement(5839..7248)
                     /locus_tag="WM481_000658"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFA9080032.1"
                     /translation="MMKNRVSNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLSSLGIQVYPGHTAEHVNGADVVVASTAVKKENPEVVAALERQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCV
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQDGDVVLNM
                     GAGSINRVPSALLELSKQI"
     gene            complement(7403..8470)
                     /gene="murG"
                     /locus_tag="WM481_000659"
     CDS             complement(7403..8470)
                     /gene="murG"
                     /locus_tag="WM481_000659"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFA9080033.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGIRGNGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPEEVRPQMYHQ
                     SGRNKLGNLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8474..9745)
                     /locus_tag="WM481_000660"
     CDS             complement(8474..9745)
                     /locus_tag="WM481_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFA9080034.1"
                     /translation="MGDGVHTLLLDRPIVRDGRKFDAPLLWMVVLMTAFGLLMIYSAS
                     VYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRTWRRLVPWIFALSGLLLVA
                     VLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREEVLRSMESLGWQSI
                     WRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQPDFGSFVVITV
                     ITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWKDPQGAGYQL
                     THSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVLIFCYGWL
                     VVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLPLMSYG
                     GSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9924..11261)
                     /locus_tag="WM481_000661"
     CDS             complement(9924..11261)
                     /locus_tag="WM481_000661"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFA9080035.1"
                     /translation="MTFQNKKTLVAGLGGTGISMIAYLRKNGAEVAAYDAELKAERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAELQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVMNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDVFCRAMKRAGREVKRFSLEHEADFWLERGTGCLKQGNEDLISTQDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLN
                     LTDCVTLEEAVQTAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(11346..11726)
                     /locus_tag="WM481_000662"
     CDS             complement(11346..11726)
                     /locus_tag="WM481_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFA9080036.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWAGDVSDFRENLQAAEQGNAAAQ
                     FNLGVMYENGQGVRQDYVQAVQWYRKASEQGDAQAQYNLGLMYYDGRGVRQDLALAQQ
                     WLGKACQNGDQNSCDNDQRLKAGY"
     gene            complement(11851..12933)
                     /locus_tag="WM481_000663"
     CDS             complement(11851..12933)
                     /locus_tag="WM481_000663"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFA9080037.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAVIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(13178..13354)
                     /locus_tag="WM481_000664"
     CDS             complement(13178..13354)
                     /locus_tag="WM481_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080038.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVPAR"
     gene            complement(13357..14715)
                     /locus_tag="WM481_000665"
     CDS             complement(13357..14715)
                     /locus_tag="WM481_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFA9080039.1"
                     /translation="MKPLDLNFICQALKLPMPSENKPVSRIVTDSRDIREGDVFFALA
                     GGRFDAHDFVGGVLSAGAAAVVVSREDCAALGGALKVDDTLAALQTLAKAWRDNVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVSATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYAGLCSDG
                     MALIPQEDANMAVFKTATFNLNTCTFGVDSGDVRAENIVLKPLSCEFDLVCGDERTAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAVDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLSHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(14840..16486)
                     /locus_tag="WM481_000666"
     CDS             complement(14840..16486)
                     /locus_tag="WM481_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFA9080040.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSVGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMEKIAGDKDWREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSRTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLTGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGEWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVPGEGNNLAFIDRQSDDPAPWYAGKGKSAKNTSKK"
     gene            complement(16509..17987)
                     /locus_tag="WM481_000667"
     CDS             complement(16509..17987)
                     /locus_tag="WM481_000667"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFA9080041.1"
                     /translation="MFSKLSPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACQG
                     EYTDGRSYIPAAVANGAAFVFWDDDGRFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIIGTVGNGFWGALEETAHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINTDDGYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALSTLQEIKPQGAALWCVFGCGGNRDCG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPQDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(18012..19760)
                     /locus_tag="WM481_000668"
     CDS             complement(18012..19760)
                     /locus_tag="WM481_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HFA9080042.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAMAVLFACLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLYGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPQNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVR
                     MHSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPLSF
                     EKQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNG
                     RYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILG
                     ISPTKPLTAAAVKTPS"
     gene            complement(19821..20084)
                     /gene="ftsL"
                     /locus_tag="WM481_000669"
     CDS             complement(19821..20084)
                     /gene="ftsL"
                     /locus_tag="WM481_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFA9080043.1"
                     /translation="MNKSNFFLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRVQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            complement(20111..21067)
                     /gene="rsmH"
                     /locus_tag="WM481_000670"
     CDS             complement(20111..21067)
                     /gene="rsmH"
                     /locus_tag="WM481_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFA9080044.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIVRAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHEPLPSWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPE"
     gene            complement(21064..21519)
                     /gene="mraZ"
                     /locus_tag="WM481_000671"
     CDS             complement(21064..21519)
                     /gene="mraZ"
                     /locus_tag="WM481_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFA9080045.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVATLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLVPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            21798..23045
                     /locus_tag="WM481_000672"
     CDS             21798..23045
                     /locus_tag="WM481_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9080046.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTFIMLLPSPEARQSSLNLQTLIAE
                     EIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTPLFAQTLARQL
                     QELLPQTAGNIYEFGAGTGQLAADLLGSVSDSINCYYIIEISPELAARQKNLIQARAP
                     EASQKVVHLTALPEAFDGIIIGNELLDAIPVEIVRKNEGGLLEHIGVCTDNGRFAYSA
                     RPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQ
                     YYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYLPQ
                     SHFLLNLGITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGIDWA
                     GFRFGDICHKLQPSCRLNPLQTA"
     gene            23141..23217
                     /locus_tag="WM481_000673"
     tRNA            23141..23217
                     /locus_tag="WM481_000673"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23175..23177,aa:Met,seq:cat)
     gene            23225..23300
                     /locus_tag="WM481_000674"
     tRNA            23225..23300
                     /locus_tag="WM481_000674"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23258..23260,aa:Ala,seq:ggc)
     gene            complement(23486..24307)
                     /locus_tag="WM481_000675"
     CDS             complement(23486..24307)
                     /locus_tag="WM481_000675"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFA9080047.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTVMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(24340..24984)
                     /locus_tag="WM481_000676"
     CDS             complement(24340..24984)
                     /locus_tag="WM481_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9080048.1"
                     /translation="MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDTYLRTEHVVWRPEMLGLARMAAAVKLSGL
                     KYQANSAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(24987..25850)
                     /gene="ftsN"
                     /locus_tag="WM481_000677"
     CDS             complement(24987..25850)
                     /gene="ftsN"
                     /locus_tag="WM481_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFA9080049.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILLYLNQGGQNAFKIPA
                     PSKQPAETEILKLKNQPKEDIQPEPADQNALSEPDVAKEAEQSDAEKAADKQPVADKA
                     DEVEEKAGEPEREEPDGQAVRKKALTEEREQTVREKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAAKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTFGKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSEVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHG
                     VASLIRAIEGK"
     gene            complement(25956..27452)
                     /locus_tag="WM481_000678"
     CDS             complement(25956..27452)
                     /locus_tag="WM481_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFA9080050.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHSARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLAEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHGAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARGKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(27466..27786)
                     /locus_tag="WM481_000679"
     CDS             complement(27466..27786)
                     /locus_tag="WM481_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFA9080051.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            28274..29800
                     /gene="putP"
                     /locus_tag="WM481_000680"
     CDS             28274..29800
                     /gene="putP"
                     /locus_tag="WM481_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFA9080052.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGASTVVVWAEWV
                     KKPARAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            30052..33657
                     /gene="putA"
                     /locus_tag="WM481_000681"
     CDS             30052..33657
                     /gene="putA"
                     /locus_tag="WM481_000681"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFA9080053.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALGRLIGKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDRLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHGDVVGTVSFADAALAQKAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLILGAGD
                     TGAALTNDASIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKAAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALIHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTGLAASGVQAVVEPDSPLASYTADLDGLLQISGKPENTGVNHVAALSP
                     LDSSRKQELANRDGALIRILPSGQGLDILQVFEEISCSINTTAAGGNAGLMAVAD"
BASE COUNT         7972 a         9790 c         8423 g         7771 t
ORIGIN      
        1 gcatttttaa tatcgaatca atcagcccaa ataccagaat ttcaatgccc acaatacggc
       61 aacaacccat accataggcg gcacgtcccc agtccggcga cacaaaagtt tgaccacggc
      121 atagctgatg aagccgaagg cgatgccgtc tgcaatcgaa taggtaaacg gcatgaagac
      181 aatggtcagg aacgcgggcg cggcttcagt catatcgtcc caatcaatgt ccctcgcact
      241 gcggagcatc tgcgtgccga cataaagcag tgcgggcgcg gtggcaaata ccggaacact
      301 tttcgccaat ggggagaaca tcagacacgc cagcatcaat acgccgacgg taaccgccgt
      361 caggccggtc cgtccgcctg ccgatacgcc cgccgcgctt tccacataag gcgtggttga
      421 agaagtaccc aaagccgcac ctgccacaat ggcggtagag tctgcaagca gtgcgcgttt
      481 caggcggggc agcttaccgt ccaccagcag tccggcacgg tgggatacgc cgaccagcgt
      541 tccggtactg tcaaacaaat cgaccaagaa gaagacgaaa atcacgctga ccatgctgac
      601 ggtaaacaga cctttaaaat ccatctgcat aaaggtcggc gcaatgcccg gtacttcgcc
      661 gaccacgccg tgaaactcgt tcaaacccat caggctggca atgacggtaa tcgtcagaat
      721 ggtgatgatg attgcgcctt gaacgcggaa ataccccaat acgaccacca tgacaaaacc
      781 gaacaatgcc aacagtgcgc tgggctgatg aatatcgccc aagccgacca aggttgccgg
      841 attggcaacg ataatgcctg cgcctttcag ggaaatcagt gccaaaaaca aaccgatacc
      901 ggcggcaatc gacattttca aacccatagg cagtgcgttg accagcattt ccctgacttt
      961 aaaaaagctg aacaggatga aaatcagacc ggaaatgaac accgcaccca acgccacctg
     1021 ccaaggcacg cccataccct taacgacggc aaaggtgaaa taggcattca gccccatccc
     1081 cggggcaagc gcaatcggat agttgccgat aaaacccatg acaaaacagc cgatggcgga
     1141 tgcgatacag gtggcgacga ataccgcccc catatccatt ccggtctcgc ccaaaatcag
     1201 gggattgacg ataacgatgt agcacatcgt caaaaaggtc gtcaaacccg ccatcaactc
     1261 ggtacgtacc gtcgtaccgt ttgccttcag gttaaaaacc ctatccagca atgtttgttt
     1321 tgaaatatcc atatctagca gtccgtttca agtaaaaaat ctgaacccgc acaccgcaaa
     1381 ccgcacagga ttcggcatat tgaaaaatat acctcccaaa tcgggaaaac cggttcggga
     1441 ggcaataagt ttacggcaga cggcacatta tttgtctgaa ttgtgttgac gacgcaaaat
     1501 tgcaggaatt tcaaaatcgt caaggacaga ctggttatcg aaatccgctg ctgtcagatt
     1561 catggtgcgg ataccgcgat tggtgcggat cataccttcg acattgtggc tttgctcctg
     1621 tttggacggg gcaaccgctt ctacttccct tgccggagta ggatcgaccg ctcctttttc
     1681 tttcagaccg gtagcgataa tggtaatccg gatggcatct tcgctcatgg tctcgtcttc
     1741 agcggcaccg aatttgcatt ccaaatcggg atgcgcgctt tggttgacga ttttcatgac
     1801 ttcagacaac tcggacattt tcaggcaacc cggagcagtc gtaatattga ccagcacacc
     1861 gcgtgcacca tccaaagtta catcgtccag cagcggactg gaaatggcct ggtcggtcgc
     1921 catacgcgca cggtcgatac cttgggcata acccgaaccc atcatagcga taccgcggtt
     1981 gctcatcacg gttttcacgt cggcaaagtc gaggttgatg atttcgctcg ggcaagttac
     2041 cacttcggaa atgcctgcga ccgcatcgcg caatacattg tccgccgcgc ggaaggcttc
     2101 gcgcatagtt acgtcttcac ccaatgcggt catcagtttg tcgttcggga tgataatcag
     2161 cgaatcgacg tgttctttca actgttccaa ccctgcctgt gccacatgga cgcgtttgcc
     2221 ttcataagaa aacggacggg taactacggc aacggtcaga atgcccaaag acttggcaat
     2281 ctcagcaaca accggcgcgg aaccggtacc ggtaccgccg cccataccgg tcgtgataaa
     2341 cagcatattg gcaccgcgaa tggcttcttc aatggcctcc cgatcttcct gtgctgccgc
     2401 acggccgata tcgggattcg cacccgcgcc caaaccgcgt gtcagattcg tacccaactg
     2461 gattctcttc gccgcatggt tttttgccaa agactgcgca tctgtattgg cactgataaa
     2521 ctccacactg cgcacattgt tggcaaccat gttattgatt gcattgcaac cgccgccgcc
     2581 caagccgatt actttaatta ccgcagggct gactgccgat tctgccacgt cgtaaacaaa
     2641 ttccattcaa aaactcctga tcgccccgtt cagaggacgg tttaaataaa ttattattta
     2701 ttatataaga agtatcttac tgctggcaaa atacttctca cctgtcaaac ggcaatccaa
     2761 ctgttcagag gttgttttca atccaccgct tcaatcttgc caacaaaccg ccgccccctt
     2821 ctctctcttg cactgtaccg ttttccggtt gcggcaagtt tccttccagc ttgcatgctg
     2881 catgaagcag cccgatagcg gtagaaaagc gcggtgtgcg gacacggtcg gacaaaccgc
     2941 ccatttcttg gggcgcaccg gtgcgtacag gcaaatcgaa gattttttcg gcaaattcca
     3001 caatcccggt catcatggac acaccgccgg tcagaacgat acccgcattc agcacttctt
     3061 tggggaaacc cgatttttgc agttcgccca gcactacgcc aaaaatctcc tgaatacgtg
     3121 cgctgatgat tgctgccaaa accttactgg aaacctgacg cgatgtccgg tcacccacgc
     3181 ccggaacttc aatcatctcg cccaagcctt ccgtatcgca tgatgccacg ccataatgga
     3241 ttttaatgta ctcggcggca tcgagaggtg ttctcaacga tttggacaaa tctttggtaa
     3301 tcagattacc accggccgga atgacggacg tatggcggat ggcgccgttc atataaacgg
     3361 caatatcggt cgttccgcca ccaatgtcaa tgacgcatac gccgaggtct ttttcatctt
     3421 cagtcagcac cgcctgcccg cttgccaacg gttgaagcat gatctgatcg cttttcaagc
     3481 cgcacagctc gatacatttt tggacattct gcactgccgt acttgcaccg gtaatgatgt
     3541 gcacccgcgt atccagacgc acaccgctca taccgatggg ctcccttaca ccaagttggg
     3601 tgtcgataat gtagtcttga accacggcat cgagaatttt ttgatcgggc gggatattga
     3661 ttgcctttgc cgtttcaatg gcgcggtcga tgtctgcctg tgttacttct ccgtctttga
     3721 ttttaaccac accttgcgaa ttaaggctgc ggatgtggtt gcctgcgata cctgtggtaa
     3781 cgtgggtaat tttggtatcc gccatcagct cggcatcatt gaccgcctgc ctgatggctt
     3841 ggacggtggc atcgatattg gttaccatgc ccgcgcgcaa gccccgtgaa ggagcctgcc
     3901 ccaaaccgat gatgttgatt ttgtcgtcat cttgaacttc cccgatcagt gcgaggactt
     3961 tagacgtacc gatatccagt acgctgatgt atcttttctg ctgttccatt gttcgtctgc
     4021 ccttaaaact gattgaaatt tgcgtcgcac cgtttcagac ggcacggccg taatatgtcc
     4081 gatacctgtt cccactattc ttcggattct ttttcgggta aaccgtcggg agcatggcgg
     4141 actgaaaatc cgtccttata cctcatatcc acataggata accgattttt attcttacgc
     4201 aacagatgct gccacgcttc ggtaaaaagc cggaggcgtt tcatctcgtt ttcccgtccg
     4261 agcctgacgg tgatgccgtt gtccaaaacg acattccacg ccgaacgtgc cgtataggtc
     4321 atctctttga tgcccaaacc ctgttttgcc aaaacagtcg aaaattcgtc ataacggcgg
     4381 agcatttcgg cagacgttcc ttccgcgcct ctgaataccg gcattccggg tctgtccaag
     4441 cgggcttcaa aaacattgcc ttcgccgtcc accaaggcat ggtcgcccca acgtgcaacc
     4501 ggcttgcgct cggtcaggac gacctcaacc gtatcgggaa aacggcggcg caccatgacc
     4561 gacgcaatcc acggataccg gcggtaggct tcctgtgcgc cattgatgtc cgtcctcaaa
     4621 atattcccat ggatgtattc tttcgccaaa ctgcccaatg ccttcttatc ggaataaacc
     4681 aggttgccct tcagcgacac ctgcttgacg ggcagatgat tcgaattgta aaaccaaacc
     4741 agcccggacg cagcaagcag catcgccatc atgacaagca gccagcgcgt cagccgttcc
     4801 atcgcttcgg cattatccca catgtgcggc cttcaaaatt tcaatacata aatcggcaaa
     4861 acccacgccc gtaacggcag cggatttcgg tactaaacta tggccggtca taccgggcag
     4921 ggtgttgatt tccaacagat agagtttgcc gtcggtatct ttgaggaaat cgacgcgcac
     4981 gcagccttcc gcaccgattg cctgtgcgcc gcgaaccgcc agttcgcgca tcaggctttc
     5041 ttcggcttcg gtcaaatctt ccgaaggaca ttgataaatg gtgtcgtctc ggttgtactt
     5101 ggcttcgtag tcgtaaaact cggttgcggg gatgatgtgt atgccgggca gccctttgcc
     5161 gttcaggacg gggcaggaat attcgccgcc gccgataaaa cgttcggcaa tgatttcgcc
     5221 ctgaaggtgt ttcaattctt cgtaaacgct tttcagacgg cctttttctt tgacttttac
     5281 cacgccgacg ctgctgcctt cggccgccgg cttcacaaac atcggcagac ccaatttttc
     5341 ttcgacggca tcgaaatcgg tatcatcgta cagtacggcg aactcgggaa cgggtaatcc
     5401 caatgcctgc caaatcagtt tgcagcggta tttgtccatg ccgatggcgg aggcggcgac
     5461 accgctgccg gtatagggaa tgcccaacag ttccaatgca ccctgaacag ccccgtcttc
     5521 gccgtaagta ccgtgaagga tgttgaatgc cgtctgaaag ccccgctcct tcagttcgga
     5581 taacggcgtt tccttagggt cgaaggcgta tgcgtctatg cctttgcttt ttaaggcgtt
     5641 caaaatagcg gtaccgctgt ccagcgagat ttctcgttcg ctggaaaaac cacccatcaa
     5701 tacggccact ttgccaaaat tctgcattgt ttttgttctt tcctaattgc tttatgcttg
     5761 ttgtcagagg tcgtctgaaa cctgattgcg gtttcagacg acctttatat gatgttccgt
     5821 ctgtcaggcg ggtgtatctc aaatctgttt cgacaattcc aacagcgcgg agggaacgcg
     5881 gttgatgctt cccgcaccca tattcaacac aacatcgcca tcctgtaaaa cattcatcag
     5941 catttgcggc aggtcggcga cgttttcgca gtaaatcggc tcaagtttgc ccaatacgcg
     6001 gatagcacgc gccaaggcgc gggagtcggc ggcggcaacc ggctcttcgc cggcggcata
     6061 aacttcggtc agtaccagcg catcaacggt attgagtact ttggtaaagt cttcaaacaa
     6121 atcgcgcgtg cgggtatagc ggtgcggctg gaaggcgagt accaaacgtt tttccggata
     6181 cgcgccgcgt gcagcggcaa gggttgccgc catttcgacg gggtggtgtc cgtaatcgtc
     6241 caccagcaaa gcggtcccgc cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg
     6301 gccgacgcct tcaaagccga gcaagccttt ttggatcgct tcaaccgatg cgccgacttc
     6361 cagcgccacg ccgatggctg ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag
     6421 cacgacttca aacgacccct gctcatgtcc tttcatttga acatggacgg tgaatttcat
     6481 ttgcgcgccg acgttttcga tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta
     6541 agtagcataa ggtttgctca ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaacaca
     6601 caaaaaggct ttgccgtaga agggcatacg gtggatgaaa tcgataaacg cctgatgcag
     6661 tttttcgacg ctgtgcccgt aggtatccat atggtcttcg tcgatattgg taacgacgga
     6721 cataatcggg gtcagatgta ggaaagaggc atcggattcg tcggcttcgg caacgatgta
     6781 ttcgcctttg cccaagcggg cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa
     6841 agtggggtcg agtcctgccg cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt
     6901 gccgtgcgta ccggcaatgg cgatgccgtc acggaagcgc atcagctctg ccagcatcaa
     6961 ggcgcgcgga ataacgggaa tttgccgctc caacgcagcg acaacttcgg gattttcttt
     7021 cttgacggcg gtagaggcaa cgacgacatc cgcaccgtta acgtgttctg cggtatggcc
     7081 gggataaact tgaatgccca ggctgctcaa atgctcggta gcggcatttc gcgcctgatc
     7141 cgaaccggaa actttaaagc ccaaattgtg caagacttcg gcgataccgc tcatgccgac
     7201 gccgccgata ccgacaaaat ggatgttgct tactcgattt ttcatcataa tgttgcgttc
     7261 cggttggttt ttagtgcgta aaggcgttat tttaaaggtc tgaccgtttg cgcaccatag
     7321 ttttctgaca aatatatagc ggattgaaat aaaaaacatc catgccgtct gaacggcttt
     7381 tcagacggca tggttcggca gtttacgccg cacacgcaat cgcggcttcg gcaacgtcat
     7441 ccgcgctgtg cggcaatgcc aacgtacggg cgttttccgc ccatttgagg catttttcgc
     7501 ggttgaggct gccgaggatt tcggcgagtt tttccgccgt caactgggtt tgcggcaaca
     7561 gcagccccgc ttctgcctgc accatgaaac gcgcgttggc ggtttgatgg tcatcaacgg
     7621 cgtgaggata cggcactaac aacgcgccca gccccgccgc cgtcaactcg gcaatcgtca
     7681 gcgcgccggc acggcaaatc accaaatcgg catcacggta ggcggacacc atgtcggtaa
     7741 taaattccac gcattccgct ttcacgccca acgcgtcata atccgcctga agattgccca
     7801 gcttgttacg ccccgactgg tggtacatct gcgggcgcac ctcttcaggc agcagtgcca
     7861 acgcctgcgg tacggttttg ttcaaaacgt ccgcacccaa actgccgccg accaccaaaa
     7921 ttttcaggcg gccttcgcgc ccttggaagc gttcggcagg cacgggcagg ttgctaatat
     7981 cggcgcggac ggggttgccg accaaaccgc cttcgtggct gaacgctttc ggaaaagcgt
     8041 acaacacccg tttcgcccag cgcgacaggt ggcggttgga caagcctgcc acggcgtttt
     8101 gctcgtgaat cacaatcggc acgcccaaga gtttcgccgc cagaccgccg ggaaaggtaa
     8161 caaaaccgcc gaagccgatg acgcactcga cacggtgttt gcggataatc cgctgcgctt
     8221 cgcggacggt tttgtacaga gtaaacggaa gcatcagctt gcgtttgatg ccgttgccgc
     8281 gtattccttt aatcgccagc gtttccaagc gtatgccgta ttgcggcacg atgcgctctt
     8341 ccatcgaatc cttgctgccc agccaaatta catgatgacc gcgcacgcgc aatgaatccg
     8401 ccacagccag agctgggaaa atgtgtccgc ccgttccgcc cgccatcagc ataaaggttt
     8461 taccgcccat gatttactcc acccggtaac cgcgcatttt ccggcggttt tcataatcga
     8521 tacgcaacag cagcatcatg ctgatcagca tgaaaaagac tgacgaaccg ccataggaca
     8581 tcaacggcag cgtcagacct ttggtcggca aagcaccgat gttcacaccg atattgaaga
     8641 aactttggat accgatccaa atgccgatac ccgaagcgat ataggcgttg aaagtcaaac
     8701 ccaaatcgcg cgactgcttg ccgatggaaa acgcccgcac caccagccag ccgtaacaga
     8761 atatcagcac gcacatcccg aagaagccga attcttcagc gatgatggca aaaataaaat
     8821 cggtatgcgc ttccggcaga aagccgcgtt tgctcaaact cgcacccaaa cccataccga
     8881 accactctcc gcgcccgatt gccatcagag agtgggtaag ctggtagccg gcaccctgcg
     8941 ggtctttcca cgggtccaaa aatgccacta cccgctgcac acggtaggga gcggcggtaa
     9001 tcatcagcac catcccaccc aagacgctgc ctaccaggac aaaaaaatat ttccacggca
     9061 atcctgccag aaacagcatt ccaacggtaa tgacggtaat gacgacaaac gaaccgaaat
     9121 ccggctgtac cattatcagc accaaaccga acgccaccag cataatcggc aggatgatcg
     9181 cccggaaacg gccgtacatt tctaatgttt cacgacgtgc ctgcggattg gtggcggaca
     9241 taatcaggtt ggccgtcccc cgccaaatcg actgccaacc caaactttcc atgctgcgca
     9301 acacttcttc acggcgcgtg aacaggcttg ccaaataaag gatgactgcc agcttgaaca
     9361 gctcggtcgg ctggaaattc aacggaccca aaggtatcca acgggtcgcg ccattgattt
     9421 cgcgcccggc aatcaatacg gctaccagca acaggccgga taaggcaaaa atccacggca
     9481 caagccgccg ccatgtcctc atcctgcaaa gaaaccataa aaaaccgctc gctataaggc
     9541 cggcaacgac gaaccccgcc tgcctggtca aatagaaaaa ctgatcgccg ccttccttcg
     9601 atgccaaata cacagaagcc gaataaatca tcagcaggcc gaacgccgtc atcagcacca
     9661 ccatccacaa aagcggcgcg tcgaatttcc tgccgtcgcg cacaatcggc ctgtcgagca
     9721 gcagagtgtg gacaccgtcg cccactttta ccaatacttc cgaaatcttc aaaaaaccac
     9781 ctgccagtct gtttgcacct gccgcaaagg gcaaaaattt cagacggcag acaatgccgt
     9841 ccgaacatac gatacatccc aaatcggtat tctaaatctt tacttgccgc ccaacaatga
     9901 cggcgtttgt gtttcggacg gcatcacaaa gccttaaacg cttcgataaa cacttccgaa
     9961 cggtgcgcgt agcctttaaa catatcgaaa ctcgcgcagg cggggctgag caagacaata
    10021 tcgcccgctt cggcttgggc gtatgccgtc tgaaccgcct cttccaaagt gacgcagtcg
    10081 gtcaggttca agccgcagcc gtccaaatcg cggcggattt gcggcgcatc gacgccgatc
    10141 aggaacacgc cttttgcctt atctttcaac gcgtcgcgca ggggcgtgaa gtcctgccct
    10201 ttgcccatgc cgcccaaaat cacgaagagc gggttttgca aaccggcaat cgcggcggcg
    10261 gtcgcgccga cattcgtgcc tttgctgtcg tcgatgaata ccacgccgtt tttctcgccg
    10321 attttttcca cacggtgcgg caagccttgg aaggttttga cgtgttccag caatgcttcg
    10381 cgcggcaaac cgacggcctc gcacaaagcc acggcagcca taacgttggc ggcgttgtgc
    10441 aaaccttgca gcgggatgtc ttgcgtagaa atcaaatctt cattgccttg ttttaaacag
    10501 cccgtcccgc gttccaacca aaaatcggct tcgtgttcca aggaaaaccg tttcacttca
    10561 cgccctgccc gtttcatggc gcggcagaac acgtcgtccg cattcaaaac ctgcactcca
    10621 tcgccacgga aaatcttggc tttggtatgc gcgtagtcga gcaaatcgtc gtagcggtcg
    10681 agatggtctt cggaaatgtt catcaccgtc gccgcagtcg ggcgcaggct ttcggtgttt
    10741 tccagttgga agctggaaag ctccaacacc cacacgtccg cctttttgcc ttcgcgctgc
    10801 aattctgcct ccaaaaccgg cgtaccgata ttgcccgcaa tgacggtatc cagcccgcac
    10861 ttgatgcaga gatagccgac caggctcgtt accgtggttt tgccgttgct gccggtaatc
    10921 gcaatcacct tgtcgccgcg gcggttcaca atgtccgcca gcaattcgat gtcgcctagc
    10981 acgcgcccgc cgttttgctt gaacgcctcg atatccggct gccgctcgct gatgccggga
    11041 ctgagcgcca gaatatcgaa accgttgtcc aacgcatctt tcagacgacc cgtgtaaaac
    11101 accagcccgt caaacatctt accgatttgc gccacgcgct cggctttcag ctccgcatca
    11161 taagcggcaa cctccgcgcc gtttttgcgc agataggcaa tcatggaaat acccgtgccg
    11221 ccaagtccgg cgacgagggt ttttttgttt tggaaagtca ttttggtttt gtcctaaaac
    11281 aaatcatatt gggcaggaga cgtccgccct tgcccaagcc gctttcagac ggcatcgcga
    11341 gccgattaat aacccgcctt caggcgttgg tcattgtcgc agctgttttg gtctccgttt
    11401 tgacaagcct tgccaagcca ttgttgagcg agcgcaaggt cttggcgcac gccgcgtcca
    11461 tcgtaataca tcaagcccaa attgtattgg gcttgggcat ccccttgttc tgaagccttg
    11521 cgataccact gcactgcctg tacataatct tgacgaactc cttgtccatt ttcatacatc
    11581 acgcccaaat tgaattgggc tgctgcattt ccctgttctg ccgcctgcaa gttttcccga
    11641 aaatccgata cgtcacccgc ccacaccgct tggttcaagc ccaaggcaat cagggcggcg
    11701 gcaagccatt tgactgtctg tttcatggtt ttctcctgtt ttagtataag gcgggtttca
    11761 gccgccgtta acgataggac ggggcggatt gccgccgcag gtttattgcg cgttcaaatg
    11821 ccgtctgaaa ggtgttcgga cggcataggt tcagcgaatt ttgagggtac tcaaaccgat
    11881 caataccaag acgatggtaa taatccagaa acggacgacg acttgggttt ctttccagcc
    11941 tttttgttcg taatggtgat gaatcggcgc catcaggaag atgcgttttt tggttttctt
    12001 gtaccagccg acctgaagca taacggacac ggcttctacg acgaacagac cgcccataat
    12061 gacgaggaca aattcttggc ggacgatgac ggcaacggta ccgagcgcgg cacccaatgc
    12121 cagcgcgccg acatcgccca taaagacttg cgcgggatag gcgttgaacc acaaaaatcc
    12181 gaggcacgcg ccgcacatgg cggtgcagaa tatagcgact tcgttcgcgc cggcgacata
    12241 gggaagctgg aggtattggg aaaattggta gtgtccgctg acgtaggcga aaatggcgag
    12301 cccggcggca acgaggacga cggggaaggc ggcaaggccg tccaagccgt cggtgaggtt
    12361 gacggcgttg gatgtgccga cgatggtcag gtaagacaac accaaaaagc cgaccacgcc
    12421 cagcggcagg gcgatttgtt tgaaaaacgg gacaatcaaa atattgttgg cggaattggc
    12481 ggcaaggtaa aacaatgcca aaccggcgat aacggcaacg cttgactgcc acaccatttt
    12541 gaatttggcg gacacgccgt tggggtcttt ataaacgact ttgcgccagt cgtcgtaaaa
    12601 accgagcgca ccggtggcaa gcagtacgcc caagagaatc cagatatacg ggttcgccca
    12661 gttgccccac aacagggtgg acacggtaat ggcggtcaga atcagcgaac cgcccatcgt
    12721 cggcgtgccg tttttgacga ggtgggtttg cgggccgtcg gtacgcactg cctgcccgca
    12781 tttgagcgcg gtcagcctgc gtatcgtcca cgggccgaac atcagggaaa acgccaaggc
    12841 ggtcaacgcc gccataacgg cgcggaatgt ggtgtattga aaaatattca gaccggttaa
    12901 ccagttgctg aaatgtgcga gccataaaaa catggggctt ccttttttgt tttgtcgtta
    12961 tagcggatta acaaaaaccg gtacgtcgtt gccccgcccc ggctcaaagg gaacggttcc
    13021 ctaaggcgcc caagcaccgg gcgaaccggt tccgtactat ccgtactgtc tgcggcccgc
    13081 cgccttgtcc tgatttttgt taatccgcta tacatcgggc ttgcgcccgc ttgaggtttg
    13141 cttgctgtcc ggcaaggttt cggacggctt ttgcagatta acgtgcaggg actttcccaa
    13201 tcacgcaaat cgggtagctc cccggatttt tgccgccgcc caagtctaaa cataaatctt
    13261 cataaagata cgcctgcgtc cgcattccgg cataaaacgc gccgaccagc gcggcaatca
    13321 gcaataaggc tttaaaaaaa cgtcggcttt tcattttcac ttatcctcca atgcctcgac
    13381 cacttcttcc atctgcataa agcgcgaacc tttcaccaac acggtggcgc gttcgggcaa
    13441 atcgtggctc aacacttgaa tcaacgggtc tttggcggcg aaccacaaac cgtccgcgcc
    13501 aaatttttcc gccgcttcga cgctgttgtc gccgacaaaa taagccgctt cgatgccttg
    13561 gtcgcgggcg tacgcgccga cttcggcgtg catggcggcg gcttcgtcct cgcccagttc
    13621 gcccatatcg cccatcacga aaatgcgcgg cgcaggcata cgcgccaaca cgtcaaccgc
    13681 ggctttcata ctgtcgggat tcgcattata agtatcgtca atcagggttg cgcccttgat
    13741 gccggctttg acgttcagac ggcctttgat gttgctgaag ccttgcaaac cttccgccac
    13801 atcgttcaaa ctcaaaccgg cagccaaagc cagcgcggcg gcagcggcgg cgttgtggac
    13861 attgtggcgg ccgggaacag gcagcaccac ggcagtgcgc tcgtcgccgc acaccaaatc
    13921 aaattcgcac gacaaaggtt tcagcacgat attttccgcg cggacatcgc cgctatcgac
    13981 gccgaaagtg cacgtattca aattaaacgt tgccgttttg aagacagcca tattggcatc
    14041 ttcttgagga atcagtgcca tgccgtctga acataagcct gcataaatct cgcttttcgc
    14101 tttggcaata tcgcccactc cgtcgaaacc gcatccgaca tgggcgcgca gggcgttgtt
    14161 gaccaaagcg gcatcgggtt tggcgatttg cgttaaaacc gccagttcgc caaaatggtt
    14221 catgcccatt tcaatcacgg catagcggtg tttttcgttt aatttcaata aagtcagcgg
    14281 caatccgatg tggttgttga agttgcctgc cgtcgccgaa acggcatcat cgccgaaacg
    14341 gcggcgcaat accgcagcca gcatctcctt caccgtcgtc ttgccgcccg aaccggtaat
    14401 gccgaacaca aacgggttca cattatcgcg ccacgccttc gccaacgttt gcaacgcggc
    14461 aagcgtgtca tcgactttca acgcgccgcc caaagccgcg caatcttcgc gcgaaaccac
    14521 aaccgccgcc gcgcccgcag acaatacgcc tccaacaaaa tcatgcgcgt caaaccgccc
    14581 gcccgccaat gcgaaaaaca catcgccttc ccgaatatcg cggctgtcgg ttacgatgcg
    14641 cgacacgggt ttgttttcag acggcatcgg aagcttgagg gcttggcaga tgaaatttag
    14701 gtccagtggt ttcatatttg ctttcgttaa tattcgggcg gcggacacat cggtagcggc
    14761 tgattttttt atcgcctgtt ttgctgtggt aaaacacaga ttattttccc attctcattc
    14821 ggcatttttt ctgtacgtat cattttttag acgtattttt agccgatttg ccttttcccg
    14881 cataccacgg cgcggggtcg tcggactgtc tgtcgataaa ggcaaggtta ttgccttcgc
    14941 ccggcacatc ggggacattc ccccaaaaat catagccgtc atcgggcaac tcgtcggttt
    15001 cgatacccgt ccaactgccg aatccgcgta aaaaattaaa cgcgctgcgc ctcactttaa
    15061 tcatttcgcg ccgcgtgtca tcggacgaaa ttttaaccag cggcacgccg tagctttgac
    15121 gcttccactc gccgtgtgtc aacgttcgct cgccgccttt gccgacatgc atcaaaccgt
    15181 ggtcggaaaa gtaaaccaaa gaccagcttt ctttattttc attcaatatc ttaaccgtat
    15241 cttctaaaaa tttatcggtt tgcgcgatgg tggaaacata gcaggatatt ttttcagttt
    15301 gatactgaaa ccgccgcgca tccttatcca aacgtgtgca aaaatcactg tgcgaaccca
    15361 tcaggtgcat cacaatcagc cgaggcttcg tgcctgcatt tcctgtcaaa acccgtttga
    15421 acgccggcaa caaaaggcgg tcgctcaacc ccgcgctttt gccataatca cccctttggg
    15481 taaaccacgg ataatcgctg cgtagggcat aggtggaaat ttcgttggca aaatgcccca
    15541 acattccttg attagacagc cacgccgtcc gaaaacccgc ctgcttcgcc aagctgacaa
    15601 tgttattgtt cggctctccc ggcagcccca aagtctgcgg cagcgaaaga ttcgtcgcgt
    15661 gggcggtcga ttggtaaccg tttatcaaca gccctttggt ccgactcaaa aaaggcgtat
    15721 cgggcaatgg gaaaccgtaa acattcatat aatccgaacg cgcgctctca ccgataacca
    15781 caacataatt tttatacttg ggcgcaacat gacggatatg ccatgttgac gctttttttg
    15841 ctgcttccaa aatgtgggcg cgcttggcgg catattgtgc cggcgcggaa gccaaatcgt
    15901 aatacaggtc gaaaatattc aacaacaggc cggcatcagg ttctcgccaa tctttatcgc
    15961 cggcgatttt ctccatcacc gcgcaggata aaatcaaagt caataatatg gtcagccata
    16021 ttttgctgcg gcgtttatag ttttttacgt cagcaaatac ccccacagat acacaatatt
    16081 tccaaactgt caagccaaaa atgaaaaata atgcctgcac aaaataaagc gacccgggaa
    16141 gattgccgac aaattcacgc gcctcggcag gattgctttc caatatcgaa ccgactatct
    16201 gataagaagg cgcaccatac agccagccga ccggcaaata tagggcggtt gtgccgacgt
    16261 aaatcagtaa aacaactgaa gaaacgcgcg ggaagccgcg tgccaataat aataaaaata
    16321 caaccgaagc caaagcgccc acggcagaat aacggtagcc gtattcatat tccaagtgat
    16381 aacctgatgc gatggccgcg ccaaaacaaa aggctaaaag cgcgcaaatg cccaatgtat
    16441 tttttaatgt ctgatccata ttttttattc gggcggattg tttcatacga tgccgtccga
    16501 atatcccatt atttacgagt taacaaagcc tgcccgacga tttcaagatc ggaaaaacgg
    16561 tgcttcacgc cctgtacatc ctgatagttt tcgtgtcctt tgccggcaat caggatgatg
    16621 tcgtttgcgg cggcctgctc aaccgcataa cggatggcgg cggcacggtc ggcttcgacg
    16681 cattcgggcg cgggcacggc gggcaggatg tcgttgatga tgtcttgcgg attttccaaa
    16741 cgcgggttgt cgctggttac gacgacttta tccgcgccct gtacggctgc cgcgcccatc
    16801 aatgggcgtt tgccgcaatc gcggttgccg ccgcaaccga atacgcacca taaggccgca
    16861 ccctgcggtt tgatttcctg caaagtggag agtgcttttt ccaatgcgtc gggcgtgtgg
    16921 gcgtaatcga cgacgaccaa gggcttgccg ctgttcatga tgcagtccat gcgccctgaa
    16981 gcgggacgga tttttgccag cacatccaat accttatcaa gcggataacc gttggcacaa
    17041 agcaaggcga tgcaggcggc gaggttttgc gcgttgaacc gtccgagcag gcgggtgcgg
    17101 cattttcctt cgccccacgg ggttcggaat acggcttcta taccgtctga agaggcggtg
    17161 aaatcggtaa tgcggatgtc ggcgtgttcg ctgaaaccat agctgtaaac ggctaaatcg
    17221 ggacagtctt ttttcagacg acctgcgagt tccgcgccgt atccgtcatc ggtattgata
    17281 acggcgtgtt tcaagccgtg ccagtaaaac aggcgcgact tgatagcacc gtaggcttcc
    17341 atcgtgccgt ggtagtcgag gtggtctcgg gtgaggttgg tgaagattgc gctgcggaac
    17401 ggcacgccgt tgactcgcga ctggtcaagg ccgtggctgg aaacttccat cgcggcggct
    17461 gttgcgcctt gttgacggaa acggtagagc agggtttgga catcgacggg ggcgggggtg
    17521 gtgtgcgcgg tttcttccaa tgcaccccaa aagccgttgc cgaccgtgcc gataatggcg
    17581 gttttttcgc ccaacaaatc ggcggcttgc gccagccatt gcgtgatgga ggttttgccg
    17641 ttggttccgg ttacgcccca gactttgaga ccgtctgaaa cgttgccgta aacctgtgcc
    17701 gccaatatgc cggcacggtg tttcaaatct ttgatgcctt gattggggac tttccattcg
    17761 ggattccacg cgaatctgcc gtcgtcgtcc caaaaaacaa aagccgcgcc gttggcaacg
    17821 gcggcgggga tataactgcg gccgtcggta tattcgccct gacaggcaac gaaaatatcg
    17881 ccttgtttga tttggcggct gtctgaatgc aacaaacgcc ctgccgcgtt tgcacacgac
    17941 agggtcggga tgccggtttc agccaaaggg cttaacttgc tgaacataaa acaatctcgt
    18001 tgatactcgg attaagacgg tgttttgacg gctgcggcgg tcagtggctt ggtcggggaa
    18061 atgcccaaga tgttcaggct gccgcccata atttttttga agggcggccc tgccactacg
    18121 ccgccgtaat agccgtgggc agtcggttcg tcgatggtaa cggccacaat cacacggggg
    18181 tttttggcgg gggcaaaacc gataaacgta ccgacgtgtt tgttgtccac atagcggcca
    18241 ttgaccagtt tgcgcgccgt accggtttta gcgccgacat cgaaaccgtc caccgcaccc
    18301 gccgtaccgg tgccgcccgg ctcggttacg gaaaccatca gattgcgtac ctcgcgcgcg
    18361 gtcgattctt tgaatatgcg tttgccttgc ggcgcaaccg cctgcttctc aaagctgagc
    18421 ggcagcaaaa cgccgtcgtg cgtcagtgcg gtataggcgc gcgccaattg cagcaggctc
    18481 aattgcaggc cgtaaccgaa agacatcgtc gcctgttcga tgggccgcca cctgcgccaa
    18541 tttctcaaca aacctgcagt ttcccccgga aagcccgagt gcatacgcac accgatgccc
    18601 aattcatgat agaagtcata catttcttcg gcgccgaaac gcgcagacag tttgcttgtg
    18661 ccgacgttgg acgatttctg cataatgccg cgcacatcca aagaggggta aacatgggta
    18721 tcgtcgcgca cgggagacgg tccgatttta taaggctgcg tattcagccg ttcgttcaaa
    18781 tcggttttgc ccgcatccaa tgccttcgca atcacgaacg gtttgattgc cgaaccaggt
    18841 tcgatcatat cggttacggc acggttgcgc cgctgttcgc tgtctgcccg gccgggtctg
    18901 ttgggatcgt aggcgggcgt attggccaag gcgaggattt cccccgtgcg ggcatccaaa
    18961 accaccaccg ttccggcttt tgcctgatgg tattcgaccg ccttgttcaa ctcttcatag
    19021 gccaaggtct gaatcctctg atcgagggaa aggatgatgt ctttgccgtt ttgcggtgct
    19081 ttattgcgcg gggagtccaa gctgtccaca atattgccct gccggtcccg caaaacaact
    19141 tccgcgccgt cttcgccata caggctgtct tcaagcgaaa gttccaaacc ttcctgacct
    19201 ttgccgtcaa tatcggtaaa tccgatgacg tgtgcaaaca ggttgcccat cgggtaatgg
    19261 cgttttaatt ctttttcaaa tacaaagttt tccaaaccca aggctttgac ctcttcggca
    19321 accttgggat cgagctgccg cttgatccaa ataaacgact tgcctttctg ttcgagtttg
    19381 ttcctcaaaa catcgaccgg cacatcgaca agctcggaca ggcgttccaa ttgggcggca
    19441 gacggcattt ccttcatatc tttaggcacg gcaaacaggg actccgtcgg cgcgctcaac
    19501 gccaaaaccg caccgttccg gtccgaaacc gtaccgcgtg tagccggcaa tgcttgagtc
    19561 cgcacaatcc ggttgtcgcc ctgttctttc aaaaagttat acgttaccgt ctgcagatac
    19621 agcccgcggg caatcagaca ggcaaacaag accgccattg ccatcaggac gaagctaatc
    19681 cgtccgttac tggtcatcgg ctttttgacc tgctcttctt tgggcagcat ccggggctta
    19741 tattcgcttt taatcaacat ttttacttct cgttattatt atcctgatgc aggaatccga
    19801 ttccggcaca caggctgctt ctatctttga tgttccacca taaaggtatt gcccgaaacc
    19861 ggcggatgga ggttttgttt ttctgccgcc gccctgatcg cttcgtgatt cgccaaacgc
    19921 gcctgttgca ccctcatttg cgcataatcc tgctccaagg cgatttcctg ttttttcgcc
    19981 ttgtccaaag ctgtgaagtt gagcctgtac tggttttgct gcatcacaac ggaaaaagcg
    20041 gaaacgcaca ccgcaagcag cagaaagaaa ttcgatttgt tcattgccgt tcagacgttt
    20101 ttctctgtta ttattccggt atcggaccgg cagtccgctc cgccacacgc aaaactgcgc
    20161 tcctcgccct cgggttggcg gcaatttccg cttcacccgg ctttaatgcc ctgcccacga
    20221 ttttcagggg cggctcgggc aaatccgctt ctctgaccgc cgcccagctc ggcaggggct
    20281 cgtgttgcga atattttttg acaaactgct tcacaatgcg gtcttccaac gaatggaaag
    20341 caatgaccgc caaacgcccg ccctctttca gacggcacat gacctgcggc aataccgccc
    20401 ctacttcttc aagctcgcgg ttaataaaga tgcggattgc ctggaaggtg cgcgtcgcag
    20461 gatcctgccc ccgctcgcga gtacggacgt tttgtgccac gatctgcgcc agcttgcggg
    20521 ttgtatcgat tggactttcc gcccgttgcg cgacaatggc gcgcacaatc tggcggctaa
    20581 accgctcttc accataattc ttgattacct cgtgcaaatc ctgttccgac gcaaccgcta
    20641 tccactctgc ggcagacata ccgcgcgtcg tatccatacg catatccaaa ggggcatcga
    20701 aacggaagct gaaaccgcgg ctgccgtcat cgatttgcgg agacgaaatc cccaaatcaa
    20761 acagcgcacc gtccaccttg ccgataccca aaccgtccaa tgccgtctga aacgaagcaa
    20821 aaccgccatg cacgacaccg acccgtttgt ccgaacgcgc cagctcttct gccacagcaa
    20881 tcgcctgcgg gtctttgtcg aaaacaatca accgccccgc atcgcccaaa cgcgacaaaa
    20941 tcagccggga atgccctccc ctgccgaacg taccgtccac atagacaccg tcttcgcgca
    21001 cggcaagcgc atccaccgcc tcattcagca agaccgtgat atgccggtaa ctttctgctc
    21061 cactcacaat tgcaaatccg tctgactcaa ctggaaggca agttcgtcag gatcgtcatc
    21121 caaagcctga accatctcag cctcccactg ctcgcgaccc caaagctcca aacggttggc
    21181 acggccgacc aaaacaactt cacggtcgaa atccaccctt ttcctcagtc cggcaggaac
    21241 cagcaccctg ccggcgctgt cccattccaa aatttccgcg ttatgcagca aaagattttg
    21301 aaaccgccgc aaaacagggt tatccgccac ttttaagttt aaaagttgcg ccgcaacctt
    21361 ttcccactcc gcaacagggt acatcaacag cttatgtttc gactcgagcg ttgccactac
    21421 ggcaggcgta tagaggcgcg acagaatgtc gcggaatttg gcaggaacag ccaaccgccc
    21481 cttactgtcg atgcttaatt cgtgtgcgcc gccgaacatg acatgcccca agccgaaatc
    21541 aaaatcacaa gggtaaaaga gacactttgc cccacaattc cccaccaatc gacactataa
    21601 gaaattttaa acactcggtc aaatcagggc atgaaaactc attaacatat ctgaaatttt
    21661 tattctgttt aaaaacaata agataaaaaa tgacgacaac ggcacggcgg gtgcagtaca
    21721 gagtgaccga accaataaac aactatatat gattaattta ataatataaa cacaatatat
    21781 agtattaaga taaagccatg acagcacccg taccaacgtg taatatgtcg ggaaatccaa
    21841 taaatttaca caagctaaca tttatcatgc tcctcccctc ccccgaagca cggcaatcct
    21901 cgctcaatct gcaaaccctc attgccgaag aaatcggcaa acacggcaat tggattccat
    21961 tttcacgttt tatggaattg gttttatacg ctccgcaata cggctactac accggcggca
    22021 gccataaaat cggcaatacc ggggatttta ttaccgcacc gaccctcacc cctctgtttg
    22081 cacagacact ggcacgccaa cttcaagaac tcctgcccca aacggcgggc aatatctatg
    22141 aattcggcgc gggaaccgga cagcttgccg ccgatttgtt gggcagcgtt tcagacagca
    22201 tcaactgtta ctatattatt gaaatatcgc cggagctggc agcacgtcag aaaaacctga
    22261 ttcaagcccg tgcaccggaa gcatctcaaa aagttgtcca cttgaccgca cttcccgaag
    22321 cgtttgacgg catcatcatc ggcaacgaat tactcgatgc catacctgtc gaaatcgtcc
    22381 gtaaaaatga aggaggatta ctcgagcaca tcggcgtttg cacggataac ggccgtttcg
    22441 cctattctgc aagacccttg cacgacccgt cgctttccac ctcggcttcc ctctattttc
    22501 ctcaaacaga ttatccctat accagcgaac tgcacccgca acaatatgcc tttatccgca
    22561 cccttgcctc aaggcttgaa cgcgggggta tgatattcat cgactacggt tttgatgcgg
    22621 cgcagtatta ccacccgcaa cgcaatcaag gcacattgat cggacactac cgacaccacg
    22681 ttatccacaa tcctttcgac ttcatcgggt tggcagatct gaccgcccac gtcaatttta
    22741 ccgacattgc ccaagccggt acggatgcag gactggattt gacaggctat ctgccccagt
    22801 cccattttct gctgaatctg ggcataaccg aactgttggc acagacgggt aaaacagatt
    22861 cggcagccta tatccgtgaa gccgccgctg ttcaaaaact gattgaccag catgaaatgg
    22921 gcgaactgtt taaagtcatc gcattcggaa aaaatatcgg catcgactgg gcaggattcc
    22981 gcttcggcga catctgccac aaactccaac cctcgtgccg tctgaatccg cttcagacgg
    23041 cataaacttt ttaacattta aaaacaagaa actaattcaa aattaaaaaa tacggcttgt
    23101 caaaaaaaca aaaaaacata taatagctaa ttcacgaaac ggcgaattag ctcagtcggt
    23161 tagagcagag gaatcataat ccttgtgtcc ggggttcgag tccctgattc gctaccagat
    23221 tttcgggggt atagctcagt tggtagagcg cttgcatggc atgcaagagg tcagcggttc
    23281 gatcccgctt acctccacca gataaaaaag cacagaccgt aaaaggtatg tgctttttta
    23341 ttgcctgatt gccggaaaat aaagaataaa ccactgcctt caaaacaggc aatcgacttt
    23401 aaacctattg ccccgcctgt cctgatttta tcagcccgcc agacaaaccc gacctgaata
    23461 atgtcttcag gtcgggttta tggtttcatt cccaacttat ccagcccgac aaccacaata
    23521 ttatgatgac aatgccgaaa acaatgcggt aataggcaaa cgggatatag tttttcttgg
    23581 aaacaaactt cagcagtgct ttaaccgcta ccaaaccgga aacaaaagcg gcaataaagc
    23641 ctatcaaaat caaaccgaca tcatgcaggg tgaaaaatcg gtaatgtttc aggacatcat
    23701 aagccgttgc tgcaaccatc atcggaacgg ccaagaaaaa tgaaaactcc gttgccgttt
    23761 tccgctcgat tccccaaagc atcccgccca taaccgtact gcccgaacgg gacgtacccg
    23821 gaaccagtgc aaacacttgg gcaacaccga tcatcaacgc atcaatcgga cgcaatgcat
    23881 caacatcggc aattttaggc tctgctcggc tttggcgttt ctccacccac aaaataaaaa
    23941 aaccgcccaa aaccagcatg actgcaacac tcaaggggtt aaacagatac tctttgattt
    24001 gtttgtcgaa caacagcccc atcacggcgg caggtataaa agcaatggca agattgagga
    24061 cgaaacggtt ggctttccgg tcttttccca cgccatgcaa cacattgctg aaacgctgcc
    24121 ggtattcaaa cactaccgcc aaaaccgcac cgagctggat ggcaatttca aaaaccttgt
    24181 gattgctgtg aaaaccaatc agattgccga acacaatcaa atgtccggtg ctggaaatcg
    24241 gtaaaaattc ggtaaaacct tctaccaagc ccatcatcag ggctttcagg acaatcagaa
    24301 aatccattgc ttgcgcttct ttcggatacg ggagttcggc tatttctgta cagcaggggt
    24361 ctgacgcttg cgttcttccc tgactttggc aaccaattct ttaatcgtat gaacgccgcc
    24421 atcaaagcca ttattgaaga taacgcggta ttttccgccg acaataacgg tcggcgtgct
    24481 gtcaataccg tattgttccg tcagtttctg catttttaat gcgacggcgg cagcttcggg
    24541 ggaatcatag gcgcgcatca gttttttgcc gtcaaaacct ttttgagata aagcccattt
    24601 tccggcaaca gccctgtttt ccaaacggat tttttgttcg taaactgctt taaacacagc
    24661 agagtttgcc tgatatttca aacccgacag cttgaccgca gcagccattc ttgccagacc
    24721 gagcatttca ggccgccaga ccacgtgctc cgtccgcaga taagtatcag acggcaatgc
    24781 cttgcccagt ttcaataaca aaggatcgaa atgatggcaa tgtacgcaaa aatagccgaa
    24841 aaattccaaa acctcaattt ttcccggctg ttcttgagga atgggtttat ccaacacaag
    24901 atagtcttcc ccttccgtca gggcatatgc ctgcgcggac aacactgccg acagcagcag
    24961 cggcaacaga tgtttaaact tcataattat ttgccttcaa tcgcacggat caggctggca
    25021 accccatgct ttttcaactc gtcctgcatt tttttcaccg catcggcaga catattgccg
    25081 ctttgcacgc ggtaaagcgt tttatgtccc gcctgatagc cgaccacttc ggaagatatg
    25141 cccaagattg ccagtttggc acgctgccct tccgcgctcc ggcggtcggc atacgcgccc
    25201 atttgcagat aatgcgttgc ttccgccttg ccaaacgttt tcattttctg cacttctttg
    25261 gcagcggcac tgcgcgcttt ttcgatgctg ccgctgttga ggatttgttc cggggtcggt
    25321 ttgggtgcaa ctttttcctt cgccgccttt ttctcttctt ttgaagcttt tttctctgtt
    25381 tctttagacg gttttaccgc ttgtttttta accgtttcgg catctttctt ctgcgctttt
    25441 tccctgacgg tttgttcacg ctcttccgtc agtgctttct tgcgcactgc ctgtccgtcc
    25501 ggctcttccc gttccggctc gcccgccttt tcttcaacct cgtcggcttt gtcggcaacg
    25561 ggctgcttgt cggcagcttt ttccgcatcc gactgctctg cctctttcgc aacatccggt
    25621 tcggacaagg cgttttgatc ggccggttca ggttggatgt cttccttagg ctggtttttc
    25681 agtttcagga tttccgtttc tgcaggctgc ttcgacggag ccgggatttt gaacgcattt
    25741 tgaccgccct ggttcagata aagcaaaata ccggcaataa tgaccgttgc cagtatcaaa
    25801 ccgaagaaga aaccggacag accttttccg gattgggaaa atttgttcat aaacatacct
    25861 taatgtgttt cagacggcat taacgccgtt tgcttacggg cggatattct aacaatatcg
    25921 ccatatttgg gcaaaacctg cttccattcc cgttcctata aagcacgacg gaagcctatg
    25981 gctttcatga cgtggcttct gccgacttct tcatcgcccg ccaaatccgc caatgtacgc
    26041 gccacgcgca taatgcggtg gaagctgcgg gcggaaagag acaatttttc cagcaggcta
    26101 cccaaggctt cctgcgcttc tttttggatg caggcttggg aatcgagttc gctgacgctc
    26161 aaagcggcgt tgactttgcc ctgccgtgcg tattgtttgc cgcgggcggc gatgacgcgt
    26221 tccaaaacgg acgcactgct ttcgcccgct tcctgctgca tcagttcggc ggcggacagg
    26281 ctggggactt cgatggtcaa atcgatgcgg tcgagcagcg gaccggaaat tttgcttcgg
    26341 taacgcgcga cactttcggg cgtgcagcgg cagggtttga cgggatgccc gagataaccg
    26401 cacggacacg ggttcatggc agcgacaagc tggaatttgg cgggatagac ggcttggcgc
    26461 gccgcgcggg aaatgtggat ttcgccgttt tccaacggtt cgcgcaaaac ctccaaaact
    26521 ttgcggtcga actcgggcaa ttcgtccaaa aataaaacgc cgtgatgcgc caatgaaatc
    26581 tcgcccggac gcggatccga gccgccgccg accatagccg ccgcgctggc gccgtgatgc
    26641 ggactgcgga aaggacggtt gctgtcgagt tgttgttggt ggttgggcag gagcgaacgc
    26701 aatgcccaaa cttctaccaa ttcgtcttcg gccagcggcg gcaggatgcc gggcagccgt
    26761 tgggagagca tagacttgcc tgttcccggc gggcccatca tcaagaggct gtgtccgcct
    26821 gcggcggcga tttccaaagc aaggcgggca gagtgctgac ctttcacatc acacaaatca
    26881 ggttgtccgc catgttcaga cggcatttgc ggtacggaac attcggtttg cgccaaaggt
    26941 tcgataccgt tcaaatgagc ggcgacttcg cccaaagagc gcgcgccgta aacggtaatg
    27001 ccgcgcatca cggcggcttg tcctgcgttt tcttcaggca aaacaaatgc acgttttgcc
    27061 tgcataccct gccacgccat cgccaacgcg ccgcgcacgg ggcgcaacaa ccccgacagt
    27121 gccaattccc ccgcaaactc gtattccgcc agcttttcgg gtgcaacctg ccccgatgcg
    27181 gcaaggatgc cgattgcaat cggcaaatcg aaacgccccg actctttggg caggtcggca
    27241 ggggcgaggt tgacggtgat ttttttggcg gggaattcaa aaccgctttg aataatggcg
    27301 gcacggacgc ggtcgcggct ttcctttact tccgtatcgg gcagtccgac gatgttgaaa
    27361 tgcggcaggc cgttggcaag gtgggcttcc acttcgacca acggcgcatt cataccgctc
    27421 aaggcgcggc tgtaaaccaa ggcaagcgac atatttcaga cggccttatt cgccggcttc
    27481 ggtttgctgc ctgatttcgg cgacggcttc ttcggcagcg gcttcagccg cttccaatgc
    27541 tgcccgttcg ggattttgcg cggcttcgag tttcgccaga cgtgcttcca aagccgccag
    27601 tttggtacgg gttttaatca aaacctgctg ctggatgtcg aattcttcgc gcgtaaccaa
    27661 atccatacgg ttgaacgcgc cgcccagcat cgccttaata tttttttcca catctttggc
    27721 agggctgttg gcgatggttt cgctgatttt cgagccgact tcttcaaaaa gctgcttgcc
    27781 gaacataatc tgcatccttc ctgaacatat caaattcaag cggctattgt ataaggaaaa
    27841 atgccgtctg aaaacgggcg gcggataatc gtcaaaacat accgcgcccc ctttgcggtt
    27901 gctaaaatta cacttcgaga cacctgccgc ctgaaaacgg atttcatatt ccgcgcaacg
    27961 cccgattaca ttacaagaca ctacaaaaca atatgctgtt ttaaatgatt tttccgatgc
    28021 gcatcgtttc aaactcggct ttttaagcca ttaagtgctt tgcaaacaac agaaattggg
    28081 ttatgctgaa acggattatt tacaatttca tatagtttca ttacatatct tattgtaatt
    28141 gaagataatt tatccgaatc ccctttcggg tatccggatt ttccgttgta ctttcattag
    28201 aaaaacattt caggcgcaag ttgcttgcaa tttcaaagcc tgtaattctg aagttttaga
    28261 cgaggaatag cacatgaatc ccatgtacat cacttttgca atctatttgg ttgcagtcct
    28321 tctcatcggg cttgccgctt atttttccac gcgcaatttc gatgattata ttttgggcgg
    28381 gcgcagcctg ggcccgtttg ttacggcgat gtcggcaggc gcgtccgata tgtccggctg
    28441 gcttttgatg ggtctgccgg gcgcgattta tttgagcggt ttgaatgagg cttggattgc
    28501 catcggcctc ttggtcggcg cgtatttcaa ctggctcttg gtggcgggcc gcctgcgcgt
    28561 gcataccgaa tatgccaaca atgcgctgac gctgccggat tatttcttcc accgcttcgg
    28621 cgcgggcgga cacttgatga aagtggtttc cgcactgatt atcctgtttt tcttcacgat
    28681 ttattgcgcc tcgggcattg tggcgggcgc aaccctgttc caaagcctgt ttgaaggtat
    28741 gacttacaat caggcaatgt ggctgggcgc gggcgcgacc atcgcctata ccttcttggg
    28801 cggcttcttg gcggtcagct ggacggatac gctgcaggct tctttgatga ttttcgccct
    28861 gattttaacg ccggtgatgg tctatctggg cttgggcggc gcggaacaga tgtctgccgc
    28921 aattcaaagc gtcgccgcag gcacgggcaa agaatacggc agcctgtttg ccggtacgac
    28981 cgtcatcggc atcatttcca ccgccgcctg gggcttgggc tatttcggac agccgcacat
    29041 tttggcgcgc tttatggccg ccgaaagcgc gaaatcgctg gtatccgcac gccgcatcgg
    29101 catgacttgg atggcgttgt gccttgcggg cgcggtagcg gtcggttatt tcggcattgc
    29161 gtatttcggt gcaaaccccg acaaagtttc ttctatgagc ggcaaccacg aacgcatctt
    29221 catcgcgctt tccaccctgc tgttcaaccc ttggatcgcc ggcatcattt tgagcgcgat
    29281 tttggcggcg gtgatgtcca ccctgtcctg ccagcttttg gtttgctcca gcgcgattac
    29341 cgaagacttc tacaaaggct ttttgcgtaa aaacgcgcaa cagtcggaat tggtatgggt
    29401 cggccgcctg atggtattgg cgattgccgt gatttccatc ctgattgctt ccgatccgaa
    29461 cagcaaagta ttgggactgg tgtcttacgc gtgggcaggt ttcggtgcgg cattcggccc
    29521 gattgtgatt ctgtccgtat tgtggaaacg catcactgcc tacggcgcgt tgtcaggcat
    29581 ggttgcaggt gcgtcaaccg ttgttgtgtg ggcggaatgg gtaaaaaaac cggctcgagc
    29641 ggcaggcgaa agcggcttgt tgacgatgta cgaaatcgtc ccgggcttta ttgtttgctt
    29701 gatcgttgcc gtgttggttt ccctgttcaa caaagaacct tcgcgcgaaa tccaagaacg
    29761 ctttgaaaaa gccgacgcag attaccgcgc cgcccgataa acccctttca gacggcacgg
    29821 catcccggtt cgtgccgtct gaacccgctt ccgggccaaa cacaaataaa ccttgcctgc
    29881 atacaggcct tatttgtgtt ttacccgttt cattttcaaa gaaacggaag caccgattct
    29941 gcccgcattt gaagccttgt atttcatctg cggatttatt acgatattac cgtattcagg
    30001 ccgcaccgat gccgtctgaa ccccccgaaa aaactttgga ggatccaaaa aatgtttcat
    30061 tttgcatttc cggcacaaac tgccctgcgc caagcgataa ccgatgccta ccgccgtaat
    30121 gaaatcgaag ccgtacagga tatgttgcaa cgtgcacaga tgagtgacga agagcgcaac
    30181 gccgcctccg agcttgcccg ccgtttggtt acccaagtcc gcgccggccg caccaaagcc
    30241 ggcggcgtgg atgcgctgat gcacgagttt tcactctcca gcgaagaagg catcgcgctg
    30301 atgtgtctgg cagaagccct gctgcgtatc cccgacaacg ccacgcgcga ccgcctgatt
    30361 gccgacaaga tttcagacgg caactggaaa agccatttga acaacagccc ttccctcttc
    30421 gtcaatgctg ccgcctgggg tctgctgatt accggcaagc tgaccgccac aaacgacaaa
    30481 caaatgagtt ccgcactcgg ccgcctgatc ggcaaaggcg gcgcaccgct catccgccaa
    30541 ggcgtaaatt acgccatgcg gcttctgggc aaacagttcg tcaccggaca gaccattgaa
    30601 gaagccctgc aaaacggcaa agaacgcgaa aaaatgggct accgcttctc cttcgatatg
    30661 ttgggcgaag ccgcctacac ccaagccgat gccgaccgct actaccgcga ctatgtcgaa
    30721 gccatccacg ccatcggcaa agatgcggca ggacaaggcg tttacgaagg caacggcatt
    30781 tccgtcaaac tctccgccat ccatccgcgc tactcgcgcg cccaacacgg ccgcgtgatg
    30841 ggcgaactgt tgcctcgcct gaaagaattg ttccttttgg gtaaaaaata cgatatcggt
    30901 atcaacatcg atgccgaaga agccaaccgt ctggagctgt ctttggattt gatggaggca
    30961 ctcgtttccg accccgattt ggccggctac aaaggcatcg gtttcgttgt tcaagcctac
    31021 caaaaacgct gcccgttcgt catcgactat ctgattgacc tggcccgccg caacaaccaa
    31081 aaactgatga tccgcctcgt caaaggcgcg tattgggaca gcgaaatcaa atgggcgcaa
    31141 gtggacggct tgaacggcta tccgacctac acccgcaaag tgcataccga catctcctac
    31201 ctcgcctgcg cgcgcaaact gctttccgcg caagacgcgg tattcccgca attcgccacc
    31261 cacaacgcct acaccttggg cgcaatctac caaatgggca aaggcaaaga ttttgaacac
    31321 caatgcctgc acggtatggg cgaaactttg tacgaccaag tcgtcggtcc gcaaaactta
    31381 ggccgccgcg tgcgcgtgta cgccccggtc ggcacacacg aaaccctgct cgcctacttg
    31441 gtgcgccgcc tgttggaaaa cggcgcgaac tcgtctttcg tcaaccaaat cgttgatgaa
    31501 aacatcagca tcgaccgcct gattaagagc ccgttcgaca ccatcgccga acaaggcatc
    31561 cacctgcaca acgccctgcc gctgccgcgc gatttgtacg gcaaatgccg tctgaactcg
    31621 caaggcgtgg acttgagcaa cgaaaacgta ttgcagcagc ttcaagaaca gatgaacaaa
    31681 gccgccgcac aagacttcca cgccgcatcc atcgtcaacg gcaaagcccg cgatgtcggc
    31741 gaagcgcaac cgattaaaaa ccctgccgac cacggcgatg ttgtcggtac ggtcagcttt
    31801 gccgatgccg cgcttgccca aaaagcggtt ggcgcagccg ttgccgcgtt ccccgaatgg
    31861 agtgcgacac ctgccgccga acgcgccgcc tgcctgcgcc gtttcgccga cctgctcgaa
    31921 cagcacaccc ccgcgctgat gatgcttgcc gtgcgcgaag cgggcaaaac gctgaacaac
    31981 gccattgccg aagtgcgcga agccgtcgat ttctgccgct actacgcaaa cgaagccgaa
    32041 cacaccctgc ctcaagacgc aaaagccgtc ggcgcgattg tcgccatcag cccgtggaac
    32101 ttcccgctcg ccatctttac cggcgaagtc gtgtccgccc tggcggcagg caacaccgtc
    32161 atcgccaaac ctgccgaaca aaccagcctg attgccggtt atgccgtttc cctcatgcac
    32221 gaagccggca tcccgacttc cgccctgcaa ctcatcctcg gcgcaggcga cacgggtgcg
    32281 gcattgacca acgatgccag catcggcggc gtgattttca caggctcgac cgaagtggcg
    32341 cgcctgatca acaaagccct tgccaaacgc ggcgacaatc ccgtcctgat tgccgaaacc
    32401 ggcggacaaa acgccatgat tgtcgattcc accgcacttg ccgaacaagt ctgcgccgac
    32461 gtattgaact ccgccttcga cagcgcggga caacgctgct ccgccctgcg tatcttatgc
    32521 gtccaagaag acgttgccga ccgtatgctc gacatgatca aaggcgcgat ggacgaactc
    32581 gtcgtcggca aaccgattca gctcactacc gatgtcggcc ccgtcatcga tgccgaagcg
    32641 cagcaaaacc tgttgaacca catcaacaaa atgaaaggcg ttgccaagtc ttaccacgaa
    32701 gtcaaagccg ccgccgatgt cgattccgaa aaatccacgt tcgttcgccc catcctgttt
    32761 gaattgaaca acctcaacga actgcaacgc gaagtcttcg gccccgtcct gcacgtcgtc
    32821 cgctaccgcg ccgacgaact cgacagcgtc atcgaccaaa tcaacagcaa aggctacgcc
    32881 ctgacccacg gcgtacacag ccgcatcgaa ggcacggtac gccacatccg cagccgcatc
    32941 gaagccggca acgtttacgt caaccgcaac atcgtcggtg cagtcgtcgg cgtacagccc
    33001 ttcggcggac acggtctgtc cggcacaggc cccaaagcag gaggttcgtt ctacctgcaa
    33061 aaactgaccc gcatccccga atgggttgcc ccgaccctga gccaaatcgg acaggcagac
    33121 gaagccgcgc tcaaacgcct cgaagccctg attcacaaac tgccgttcaa cgccgaagag
    33181 aaaaaagccg cagcggccgc tttgggacac gcccgcatcc gcaccctgcg ccgtgccgaa
    33241 accgtcctta ccggcccgac cggcgagcgc aacagcatct catggcacgc gccaaaacgc
    33301 gtttggatac acggcggcag cacggttcaa gcctttgccg cactgaccgg acttgccgcc
    33361 tccggcgtac aggcagtggt cgaacccgac agcccgctgg cttcctacac cgccgacttg
    33421 gacggtttgc tgcaaatcag cggcaaaccc gaaaacaccg gtgtgaacca cgtcgccgcc
    33481 ctaagcccac tggacagcag ccgcaagcag gaactggcaa accgcgacgg cgcactcatc
    33541 cgcatcctcc cgtccggaca aggcttggac atcctgcaag tgtttgaaga aatttcttgc
    33601 agcatcaaca ccacagccgc cggcggcaac gccggcctga tggcggttgc cgactgattt
    33661 tgccaaaccg cccgggcgcg gcccgtgaac aaatgccgtc tgaaaacctt tcaggcggca
    33721 ttttatagtg gattaacaaa aatcaggaca aggcggcgag ccgcagacag tacaaatagt
    33781 acagcaaggc gggccaacgc tgtactggtt taaattcaat tcactatatg tgttggcaca
    33841 tcgctccaaa cctgatataa tccgcctttc aacatcagtg aaaatcgttc tttttagtca
    33901 gttaacatta aatttcggag tcgaaaaatg aatccagccc caaaaaactt ctcttc
//
LOCUS       DAWTCI010000014        32645 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWTCI010000014 DAWTCI010000000
VERSION     DAWTCI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32645
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.014"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            115..364
                     /locus_tag="WM481_000682"
                     /pseudo
     CDS             115..364
                     /locus_tag="WM481_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1241..1512
                     /locus_tag="WM481_000683"
                     /pseudo
     CDS             1241..1512
                     /locus_tag="WM481_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1748..2034
                     /locus_tag="WM481_000684"
                     /pseudo
     CDS             1748..2034
                     /locus_tag="WM481_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2459..2683
                     /locus_tag="WM481_000685"
     CDS             2459..2683
                     /locus_tag="WM481_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080054.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQGSIGKTEEPSYFLMFMFL
                     NSLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            2739..3395
                     /locus_tag="WM481_000686"
     CDS             2739..3395
                     /locus_tag="WM481_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9080055.1"
                     /translation="MEQKRRFAASLLLAAALPLCAHSFPFAEENPIAYGKVKIQSWKA
                     RRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMP
                     DLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVLRGVDGNTVLLADPSPGHVSM
                     SRAQFLEAWQTREGNLAGKILAVVPKKAEAISNKLFFTHHPKRQTEFAVGQIRQGRAE
                     "
     gene            3523..4818
                     /gene="hisS"
                     /locus_tag="WM481_000687"
     CDS             3523..4818
                     /gene="hisS"
                     /locus_tag="WM481_000687"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFA9080056.1"
                     /translation="MAQKIQSVKGMNDLLPVGQKDFKLTAVFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEAQLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENSRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELADGTVTLKDMNGAHGQQTVSATDLTDTLQQWKNA"
     gene            4819..5448
                     /locus_tag="WM481_000688"
     CDS             4819..5448
                     /locus_tag="WM481_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFA9080057.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELSKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQEKSQEALKNYGQALEKMPQDSVGRELLQMKLDSLK"
     gene            5598..7055
                     /gene="der"
                     /locus_tag="WM481_000689"
     CDS             5598..7055
                     /gene="der"
                     /locus_tag="WM481_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFA9080058.1"
                     /translation="MKPTIALIGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYFVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGDRAVLAAEFYELALGEPHVISGAHGDGVYYLIEEIL
                     ENFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEKRVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDISRKLYFLDFAKFHFISALKERGI
                     DGLFESIQAAYNAAMIKMPTPKITRVLQTAVGRQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            7086..7985
                     /gene="rdgC"
                     /locus_tag="WM481_000690"
     CDS             7086..7985
                     /gene="rdgC"
                     /locus_tag="WM481_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFA9080059.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTRLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            8037..8426
                     /locus_tag="WM481_000691"
     CDS             8037..8426
                     /locus_tag="WM481_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080060.1"
                     /translation="MLKHLAFLLPAMMFALPAQTAVLSPYQETGCTYEGGIGKDGLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAAGREVFLEPFNSDSTKFRNMALSGTFK
                     QGLAHGRFAASQNGETLFYYEMRTRHD"
     gene            8521..9087
                     /locus_tag="WM481_000692"
     CDS             8521..9087
                     /locus_tag="WM481_000692"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFA9080061.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGQRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEVCETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(9153..9857)
                     /locus_tag="WM481_000693"
     CDS             complement(9153..9857)
                     /locus_tag="WM481_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFA9080062.1"
                     /translation="MLRSVLAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKAEGRDFDALNRFIADVQTDASLEDTDFSVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAA
                     PGVEEISISINGTVQF"
     gene            10024..10611
                     /locus_tag="WM481_000694"
     CDS             10024..10611
                     /locus_tag="WM481_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9080063.1"
                     /translation="MVSTIRTGCGRNIFPKSSTGPPTQRHQNRHPRPHVPLSAERNSK
                     HPDRSLRFCPIPAKRFGHLRMAQHVFLPRFPFIDIDRIRWVRAGLKAFKHYIRENGLP
                     DLIHAHCMNYAGILAQKISEKYGIPYVLTEHSSTITRGLIRHHQWQPMEKAAAPASAR
                     LAVSRHFAHVLQHKYGCEWQYLPNIPGGIFKQTFE"
     gene            10639..11196
                     /locus_tag="WM481_000695"
     CDS             10639..11196
                     /locus_tag="WM481_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9080064.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGIAHAVTFLGALQPEAILDLMRNSDAFILASRTETFGVVYIEALSQGLPV
                     IAIHCGGAESIVSDGNGYLVSVDDADVLAFLKIYEHHADFSAEQLRTDCLATFGEDAV
                     IGRLIAVFRQAARNTARKRPKNRLK"
     gene            11222..12175
                     /locus_tag="WM481_000696"
     CDS             11222..12175
                     /locus_tag="WM481_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFA9080065.1"
                     /translation="MTHTVHLHLEETDNPTLQRLCGSFDSNLDSLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDSIIRLAAVETQTEDAGHQEKNHEHAYYF
                     RTKRGSIGGRTPRQNGYIRALLDHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEITPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVAGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(12280..12603)
                     /locus_tag="WM481_000697"
     CDS             complement(12280..12603)
                     /locus_tag="WM481_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080066.1"
                     /translation="MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCGDEAIR
                     CRKFD"
     gene            complement(12776..14134)
                     /locus_tag="WM481_000698"
     CDS             complement(12776..14134)
                     /locus_tag="WM481_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFA9080067.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNISAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGNAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDIHPLLNALRVSDGIAGKM
                     TALALKNTDERLRADKSVSVGFVLAALMWPELDRHWKSNLQQGLKPTPALSDAINTMR
                     ETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAETG
                     NADRALAEWWTAFQTASAEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGTD
                     WE"
     gene            complement(14492..16192)
                     /gene="recJ"
                     /locus_tag="WM481_000699"
     CDS             complement(14492..16192)
                     /gene="recJ"
                     /locus_tag="WM481_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFA9080068.1"
                     /translation="MSVKIQTRPVNTDVFNHLLAAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQSLTNCEAAAGRLADAVERKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLTALRAELRRRNYFSDGIKEP
                     NLGGLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAARLNNLNIERREIEQSM
                     LRDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTYITDGSLPACDITLEQAQNLACHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRDNLELQLYIDYWEAA
                     "
     gene            complement(16261..16974)
                     /locus_tag="WM481_000700"
     CDS             complement(16261..16974)
                     /locus_tag="WM481_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080069.1"
                     /translation="MLFRKTTAAVLAATLILNGCTMMLRGMNNPVSQTITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFAVNPEDSAKLTGLLKAGLDKPFQIVEDTPS
                     YARHQALPVKFEAPGSQNFSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     AAVLVLPMALIAAANSSDK"
     gene            complement(17021..17611)
                     /locus_tag="WM481_000701"
     CDS             complement(17021..17611)
                     /locus_tag="WM481_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HFA9080070.1"
                     /translation="MQGVRCKKPSERGGILLKYAIGKNSRIKMFEQEDEWISKTQMKK
                     QMNGLQDLGMELTRLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRD
                     TDPAPIEAFLAKLRGDDAAHNAFLQRVEQARVRLLADEGALTQFMSDFPNADAGRLRT
                     LVRNTKKEQEQDKPPKNFRALFQELKTVMENGGAEI"
     gene            17681..19012
                     /gene="pmbA"
                     /locus_tag="WM481_000702"
     CDS             17681..19012
                     /gene="pmbA"
                     /locus_tag="WM481_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFA9080071.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALDTDSRIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGTLSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLGSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(19311..19514)
                     /locus_tag="WM481_000703"
     CDS             complement(19311..19514)
                     /locus_tag="WM481_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFA9080072.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            19759..20061
                     /gene="clpS"
                     /locus_tag="WM481_000704"
     CDS             19759..20061
                     /gene="clpS"
                     /locus_tag="WM481_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFA9080073.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLSVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            20063..22354
                     /gene="clpA"
                     /locus_tag="WM481_000705"
     CDS             20063..22354
                     /gene="clpA"
                     /locus_tag="WM481_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFA9080074.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDAAVPNV
                     LKLCGADLKAVSEQLAASVAENTPLIPDHLLDTVETRPTLGFQRVIQRAMVHTQSAGK
                     GLAEPLDVLVALMSETDSHAVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYFSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALADGLAHQIVNDDIPDALKEAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTGGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVAETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINGRFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLNNMVYGQEDAIDALVSAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTE
                     VAKQLAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHRLLDKK
                     VEAEFTPALRKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(22394..25492)
                     /locus_tag="WM481_000706"
     CDS             complement(22394..25492)
                     /locus_tag="WM481_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFA9080075.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPAENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTGFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSPDSVNGSESTAGLKRNLEKDGNKIIVTTIQKLNNLMKS
                     EDNLPVYHRQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKELSAAENRQALL
                     HPERIREITQYILSRFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKTATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNREVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNIVLEKSYEEYMNGYTDSQTG
                     EARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRKVPMPSERAVQDYRSAYNDIRDWLRRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEGLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGA
                     DI"
     gene            complement(25724..26947)
                     /locus_tag="WM481_000707"
     CDS             complement(25724..26947)
                     /locus_tag="WM481_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFA9080076.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGEVLVRTKGTKITAGQMKEM
                     HKDNAPLKIFAGGKTFALVDFDDVPDKDIHREPSIIVKSRGIIEFEYYDKPFSHKNEM
                     WSYHSVNKHIYIKYVYYFLKTQENYFRNIGSKMQMPQIATPDTDNYKIPIPSLETQQK
                     IVKILDKFTELEATLEATLEAELALRKRQYRYYRDLLLDFDNQIGGGIADGYQCRLKN
                     VVWKTLGEVAEYSKNRICSDKLNEHNYVGVDNLLQNREGKKLSGYVPSEGKMTEYIVN
                     DILIGNIRPYLKKIWQADCTGGTNGDVLVIRVTDEKVNPKYLYQVLADDKFFAFNMKH
                     AKGAKMPRGSKAAIMQYKIPIPPLPEQEKIVAILGKFDTLTHSVSEGLPHEIALRRKQ
                     YEYYREQLLAFPKAA"
     gene            complement(27114..27965)
                     /gene="dinD"
                     /locus_tag="WM481_000708"
     CDS             complement(27114..27965)
                     /gene="dinD"
                     /locus_tag="WM481_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFA9080077.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSARTLQQILEYSEWRNFQRAID
                     KAITACETSGNDKNHHFVETNKMIALGKGGQREVADYRLSRYACYLIVQNGDPSKSVI
                     AAGQTYFAVQARRQELQDEAAFRSLGEDKQRLLLRRQLREHNTDLAAAAKDAGVEKPV
                     EYAVFQNHGYRGLYGGLDKQGIHSRKGLKKSQRILDHMNASEPAANLFRATQTEEKLR
                     RKNIQGKTQANRVHFEVGQKVRQTIEELGGIMPENQPVPEKSIKQLENEEQKRLAATE
                     QHQNGKK"
     gene            complement(28081..29625)
                     /locus_tag="WM481_000709"
     CDS             complement(28081..29625)
                     /locus_tag="WM481_000709"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFA9080078.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQGIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGNFEDHRIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSIDWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTCIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEAEDTREVIDIRQLNAEISETVAKIERLRREIDEV
                     IAEIET"
     gene            complement(29700..30704)
                     /gene="rfaD"
                     /locus_tag="WM481_000710"
     CDS             complement(29700..30704)
                     /gene="rfaD"
                     /locus_tag="WM481_000710"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFA9080079.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLTKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKINLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKSELSLKEL
                     VEEELIRYIPFPDALKGKYQGFTQADITKLREAGYKEEFFDVKAGVNRYVKWMLENLA
                     "
     gene            complement(30768..31730)
                     /gene="hldA"
                     /locus_tag="WM481_000711"
     CDS             complement(30768..31730)
                     /gene="hldA"
                     /locus_tag="WM481_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFA9080080.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAAAALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHVGKTVLIDPKGDDYEKYVGATLITPNCAELKEVVGSWKNEGDL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELVEALDGQ"
     gene            complement(31786..32526)
                     /gene="pyrF"
                     /locus_tag="WM481_000712"
     CDS             complement(31786..32526)
                     /gene="pyrF"
                     /locus_tag="WM481_000712"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFA9080081.1"
                     /translation="MNPLITDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPALCQIK
                     IGKELFTATGRSLAESLIHQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGRDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTRAADPVAVLREVNRVANLEAN"
BASE COUNT         7769 a         8570 c         8213 g         8093 t
ORIGIN      
        1 aaaatcccgc actcatcgct ttcctcgaaa gcacggttta cgaaaaatcg cgcaacaaag
       61 cctcgttaat caaaaaaagg gggctttgcc ccttttataa cttaagggta aattatgaat
      121 attaaaaatg aaatagttgc tttaatttct aaaaaaattg atgggggggc aatatagttc
      181 tttagaaaat tttgctttat ccgtactaag ggaaaaaatt ataaacaata ctgtttccag
      241 tgatgaaaaa ttattgatta ttaatgattt tttatcaggt tttcttgagt ttgatcctga
      301 gacaggggaa cctgtcgggg agacgcttaa aattgagcaa atgattgatt tctttttagt
      361 ctaaatcata cataccagtg gacaagctag gcgtagatcc ggcttttaaa tacgattgtc
      421 aggtttatac ttgggataaa atcaaaaata aagttgttta aactatagtg gattaacaaa
      481 aatcagggca aggcgacgaa gccgcagaca gtacagatag tacggaaccg attcactcgg
      541 tgcttcagca ccttagggaa tcgttccctt tgagccgggg cggggcaacg ccgtactggt
      601 ttttgttaat tcactatatt gcatggtgca tgcattaaat cagtcttcta gcgccgcaat
      661 gggaaaacat agaagataaa cgaaatggat gatctaatac tatatttttt atcaggtata
      721 ttcggaaatc aagttgctga atatattata aaaaacaacc gcgagataaa agttcccttt
      781 attgcgcttt acgctatatt ttttacgttg atttatactg tggctttgtt gtttctcagt
      841 ctgatctatt gggtcaatgg tgcggagatt gcatggaagg ggataggtat tttcagtatg
      901 tcggtcagtt tttgtatagt cttctgtctt tatttggttg acaaggcagg aagatgtaag
      961 gataagaaac aatagggatc agatgcggca gcaaacgtag attgggtcta ggctcaacct
     1021 gcgtttgctg catctttgga agaaatagtg gattcgaaaa aataggaaga attctgatta
     1081 atggtatgcg aggtgtagca gtgggggctg ttgctggtgg cataactggt tatgcaggtt
     1141 ctacgggtaa gaacactgac attcgccgtt aaataataaa taaaccaact tccaaccaaa
     1201 cctgctatcg gtttggttgg aagtgagagg aggtaaggta gtgtttgctg ttattttctt
     1261 ttcgacattg ggctgtattt tagcatggat aagagatatt cctaaaataa aatcaaaaaa
     1321 aattttggca agatccttat atattatagg aataataaat gtaataatca gctatgtatt
     1381 aattaaaaat atattggttt ctgtttcgga tggaggtgga ataaaatatg ttgcaatata
     1441 tttatcaaat cttttttttg gacagtattg atgtatgctc ttgtgaaaag attatctaaa
     1501 aagccaagct gaaacccagt acacaacaca tcaataaaga ttccttttgc acggaacaat
     1561 gacaatattc aaattgctgg atttgcaatc tgatttacct attgaaattg ggtgtgcaac
     1621 atacgttcta agcaggcttt gatttcccaa agaggctgaa gggtaagaga cgcttatatc
     1681 atagataaat aatggcaaaa caggtaatga attaaaggca ttacctgttt ttacagaaat
     1741 ttttaaaata gatgatttga ttgcagcttt ttaggctatt tatcaggtaa atacagtgaa
     1801 gggaaaataa gcaggttaga aatatcccta acggcaggaa gcattttctt tgcagtattc
     1861 tttattcatg aactgataat ttttattgtt gatggaaata aggaaaatct ttggaatttt
     1921 attactggga taatgaaaga tttattttta ttttcatgtt ctgtttgttt attattctat
     1981 ttcttattat tgattgcaga aaccattgga aaaacaatca ataagattaa ataagatatt
     2041 taagccgcta aaaaaccatt tgagtatttt atcttctatg cggcatccat cttggaattg
     2101 aaatactgca atcgggaggc taaggcttcc gttgtaacgt ttaccatata tttcattatt
     2161 ttaatttcat caaaaaacaa aattcaagat tatacatacc gccaatcgtt tcttgattgt
     2221 tttgtatagt ggagtaaatt taaatcagga caaggcgacg aagccgcaga cagtacaaat
     2281 agtacggaac cgattcactc ggtgcttcag caccttagag aatcgttctc tttgagctaa
     2341 ggcgaggtaa tgccgtaccg gtttaaattt aatccactat atatttatcc gatatatcaa
     2401 catctctcta tcttattccg aattttattg ttgaaacttg cattaaaaac catacaagat
     2461 gatgaagaac aaatatattt taataaaagc agcaatcggc ataaccgcca tatccatatt
     2521 ttatcttctg actcaaggaa gcatcggcaa aactgaagaa ccatcatatt tcttgatgtt
     2581 catgtttctc aactcgcttt ggtttgaaga aaataaaaca gttatggctg ccgttacggc
     2641 aatgattgcc gcccacttta tatttgtcgc actatccgat taataagcaa acaaaaaggc
     2701 tgcttggaaa tttcaggcag cctttcagga ggattttaat ggaacaaaaa aggcggtttg
     2761 ccgcctccct gctcctcgcc gccgccctgc cgctttgtgc acattctttt ccctttgcgg
     2821 aagaaaaccc catagcctac ggcaaagtca aaatacagag ttggaaagcg cggcgggatt
     2881 tcaatattgt aaagcaggat ttggattttt cctgcggggc ggcttcggtg gcgacacttt
     2941 tgaacaattt ttacgggcaa aagctgacgg aagaagaagt gttggaaaaa ctgggtaagg
     3001 aacagatgcg cgcgtcgttt gaggatatgc ggcgcattat gcccgatttg ggttttgagg
     3061 cgaagggcta tgccctgtct ttcgagcagc tcgcgcagtt gaaaatcccc gtcatcgtgt
     3121 atctgaaata ccgcaaagac gaccattttt cggtattgcg cggagtggat ggcaatacgg
     3181 ttttgcttgc cgacccgtcg ccgggtcatg tttcgatgag cagggcgcag tttttggagg
     3241 cttggcaaac ccgtgaggga aatttggcag gcaagatttt ggcggtcgtg ccgaaaaaag
     3301 cggaggcgat ttcaaataaa ttgtttttca cacatcatcc caagcggcag acggagtttg
     3361 cagtcggaca aatcaggcaa gggcgcgcag agtaagtcaa atgccgtctg aaaactgttt
     3421 cagacggcat ttttacccgt ccgcaaacca tctccatcct cacaagccct taaaaatccg
     3481 ctataatcgc ccgcaatttg atttcagcac cccctttttg aaatggcaca aaaaatccaa
     3541 tccgtcaaag gcatgaacga ccttctgcct gtcgggcaaa aagatttcaa actcacggct
     3601 gtgttttggc aggcgtttga ggatacggta aaccgttgga cgcgagctta cggttatcag
     3661 caaatccgta cgccgattgt cgagcaaacc ggtttgtttg tccgctccat cggcgaggaa
     3721 accgatgtag tcggcaagga aatgtacacc ttctccgatt caaacgattc tttgagtttg
     3781 agcctgcgtc ccgaaggtac ggcctcttgt ctgcgtgcgg tggtcgaaca caaccttctg
     3841 tacaacagcc cgcaaaagct gtggtatatg gggccgatgt tccgccgcga gcgtccgcaa
     3901 aaaggacgct accgccaatt tcatcaggtc ggtatcgagg ctttgggttt tgaagggccg
     3961 gatattgatg cggaaatcat cgcaatgtct gccgacttgt gggaaaaatt gggtattcgc
     4021 gaatacctga ctttggaaat caacagcttg ggcaaccgtg aggaacgcgc ggcacaccgt
     4081 gcggcattgg ttgaatatct gacccgctat gaagcgcaac tggacgaaga cagcaaacgc
     4141 cgtctgaaaa ccaatccttt gcgcgttttg gatacgaaaa accctgattt gcaggaaatc
     4201 tgcaatgccg cgccgcgttt ggtagattac ttgggcgagg cttcgcaaaa ccactatgca
     4261 cgcttcaagg cgatgttgga cggtttgggt attcaatata ttgaaaattc gcgcttggtt
     4321 cgcggtttgg attattacaa tcagacggtt tttgagtgga cgaccgacaa actcggcgca
     4381 caggcgactg tgtgcggcgg cggacgttac gacggtttga ttgaagaact cggcggcaag
     4441 cctgcgccgt ccatcggctt tgcgatgggc atcgaacggc tgctgctttt ggtgagcgaa
     4501 tacggttcgc tggaagtgaa cgccgcgcct gatgtctatg caatgcacca aggcgaaggg
     4561 gcggacttgc aggtgatgaa atacgcacaa gccttgcgcg cgcaaggttt caatgtaatg
     4621 cagcattccg gctatcaaag cctgaaggcg caaatgaaaa aagccgacaa cagcggcgcg
     4681 cgctttgccc tgattgtcgc gcaagacgaa ctggcggacg gtaccgttac gctcaaagac
     4741 atgaacggcg cacacggtca gcaaaccgtt tccgccaccg atttgacaga tactttacaa
     4801 caatggaaga acgcataaat ggcagcccat ctcgaagaac aacaagagtt agacaacttt
     4861 aaatattttt ggaaaaccac gggcaaatgg ctgtttgccc tgctgatttt ggcggcactc
     4921 ggctacttgg gatacacggt ttaccaaaac cgtgcggctt cccaaaatca ggaagcggcg
     4981 gcggtgctgg caaacatcgt ggaaaaggcg caaaacaaag ccccgcaaag cgaaatcaat
     5041 gccgaactgt ccaagctcca acaaagctac ccccattcca tttccgccgc ccaagccacg
     5101 ctgatggcgg cggcaaccga atttgacgcg cagcgttacg atgttgccga aggtcatttg
     5161 aaatgggtgt tgtccaacca aaaagacagc ctgattcagg cgttggcggc gcagcgtctg
     5221 ggcgttgtgt tgttgcaaca aaaaaaatac gatgccgcgc ttgccgcact cgacacgccg
     5281 gttgaggcgg acttcgcccc cctgctgatg gaaactaaag gcgatgttta tgccgcacag
     5341 gaaaaaagcc aggaagcctt aaaaaactac ggacaggctt tggaaaaaat gcctcaagat
     5401 tctgtcggtc gcgaattgct tcaaatgaaa ctcgattcgc tgaaataaat accgtctgaa
     5461 atgccgctct ctcttcagac ggcatttcag acaaccccat cctacggcaa ttcccgtaaa
     5521 accatccgcc gtgtcccaac ggacacctgc tcgtgttcag ttctaccgaa cggcacttga
     5581 cacagaaaga cccaatcatg aaaccaacca tcgcgcttat cggccgcccc aacgtcggca
     5641 aatctacctt gttcaaccgt ttgacgcgca ccaaagacgc gctcgtgcat gacctgccgg
     5701 gtctgacccg cgaccgccat tacggacacg gcaaagtcgg cagcaaacct tattttgtca
     5761 tcgataccgg cggtttcgag ccggttgtgg acagcggcat tttgcacgaa atggcaaaac
     5821 aaaccttgca ggctgtcgat gaagccgatg cggttgtgtt tttggtggac ggccgtaccg
     5881 gtttaacacc gcaagacaag attattgccg accgtttgcg ccaaagtccg cgtcccgttt
     5941 atttggccgt gaataaaggc gagggcggcg acagggccgt acttgccgcc gagttttacg
     6001 agctggcatt gggcgagccg cacgttattt ccggcgcgca cggcgacggc gtgtattacc
     6061 tgattgaaga aattttagaa aacttccccg agcctgaagc cgaagaagcc gatgcaaaac
     6121 atcccgtttt tgccgttatc ggtcgtccga acgtcggcaa atctacgctg gttaacgcca
     6181 ttctcggcga aaaacgcgtc atcgccttcg atatggcagg cacaacgcgc gacagcatcc
     6241 acatcgattt cgagcgcgaa ggcaaaccgt ttaccatcat cgataccgca ggcgtgcgcc
     6301 gtcgcggcaa agtggatgaa gcggtggaaa agttctccgt tatcaaagct atgcaggcgg
     6361 ttgaagcggc aaacgttgct gttttggtat tggacgcgca acaggacatt gccgaccaag
     6421 atgcgacgat tgccggtttt gctttggaag cgggacgcgc gctggtagtt gccgtcaaca
     6481 aatgggacgg catcagcgaa gagcggcgcg aacaagtgaa acgcgacatt tcccgcaaac
     6541 tgtatttcct cgattttgcc aagttccact ttatttccgc gttgaaagag cgcggtatag
     6601 acggtttgtt tgaaagcatt caagctgcct acaacgcggc gatgattaag atgccgacgc
     6661 cgaaaatcac gcgcgtattg caaaccgccg tcggacgcca acagccgccg cgtgccggct
     6721 tggtgcgtcc gaaaatgcgt tatgcccacc aaggcggcat gaatccgccg gtgattgtgg
     6781 tacacggcaa ttcgctgcac gcgatttccg acagctatac gcgctatctg acccagacct
     6841 tccgcaaagc cttcaacctg caaggcacgc cgttgcggat tcaatacaat gtttcggaaa
     6901 acccgtatga aaatgcggaa gacaaaccga agaaaaaacc gctgcgccgc gtcagcctga
     6961 gcaaccgtat tgagaaacgc gaaggccgaa aggaagagaa aaaccgcttc aagaagaaaa
     7021 ccaaagtcag tgtgaaaaaa caattcagca aataattccc gacatttcaa acaataggaa
     7081 acattatgtg gttcaagcag attagttttt atccgctcaa caaagaaaag ctgcctgagg
     7141 cggacgtact tgccgataaa cttgctgaag ctgaatttac ccattgccaa ggtttggact
     7201 ggttcagcga aggctttacc gcaccggttt ctttttcgcc cgaactcgtt ttccctgccg
     7261 actttacctt gcgcgtcgcc ctgaaaaaag aggaaaaagt cctgcctgcc ggcgtcatcc
     7321 gcgatatttt ggaagagaag gtagcggaaa tccaaaacaa tgaagcccgc aatgtcggtc
     7381 gcaaagaaaa acaagagctt aaagagcaaa ttacagacga cctgctgccc cgagcgttta
     7441 cccgcagcag ccgtacagaa gcggtgttta acacccgcca cggctacctg ctcgtcaata
     7501 acgcggcttc cgccaaagcg gaaaacatcc tgaccaagct gcgcgaagct ttgggaggtt
     7561 tggaagcctc gctgccgaat accaaacaat cgccctcttc cctgatgacc ggctggctgt
     7621 tgcaagggca ttgcgaaggc ggttttgaat tagacagcga ttgcgaactc aaaggtacgg
     7681 gcgatattgt tcccgtcgtc aaagtatcca aacaagactt aaccgccgac gaagtggttc
     7741 aacacgtcaa aaacggtaaa accgttaccc ggctcggttt ggtttggcgc gaacaaatcg
     7801 cctttatcct cactcaagac ttcacactca agcgcatcca atacctcgac gtattgcagg
     7861 aagaagccga aagcaacggc gacgatgccg ccggccttgc cttcgcctcg caaatcctga
     7921 tggcggaatc cgtcagcacc atgttggaag agctggtttc ctatttgggc ggctggcaag
     7981 attgattttt cagacggctt ttataccgtc agccaaaatc caatggaata acccttatgc
     8041 ttaaacatct cgcattccta ctgcccgcca tgatgttcgc cctccccgcc cagaccgccg
     8101 tcctaagccc ctatcaggaa accggctgca cctacgaagg cgggatcgga aaagacggac
     8161 ttccttcagg caaaggcata tggcgttgcc gggatgggcg cggttatacc ggttcattca
     8221 aaaacggcaa attcgacggg caaggcgttt ataccgttgc cgccggccgc gaagtatttc
     8281 tcgagccgtt caattccgac agtaccaaat tccgcaatat ggcattgtcg ggcacgttca
     8341 aacaaggctt ggcacacggc aggttcgccg cctcgcaaaa cggcgaaacc ctcttttatt
     8401 atgaaatgcg aacacggcat gattaaagaa gtgaaactgc ccaaaaacaa ataacccccc
     8461 gtttttcaaa cagcatcccc caatgcttta tccgaacacc gcaaaaaaca aggaaacccc
     8521 atgagcatca agtccgacaa atggatacgc cgaatgagcg aagaattcgg catgattgat
     8581 cccttcgagc cgaaccaaat caaagaagcc gacggacagc gcatcatctc ctacggtacg
     8641 tccagctacg gctacgatat ccgctgcgcc aatgaattta aaatttttac caacatcaac
     8701 agcaccatcg tcgatcccaa aaacttcgac ccgaaaaatt tcgttaccgt cgaagacgac
     8761 tgctgcatca tcccgcccaa ttccttcgca ctggcgcgca cggtcgaata tttccgcatt
     8821 ccgcgcaacg tcctgaccgt ctgtttgggc aaatccacct acgcccgctg cggcatcatc
     8881 gtcaacgtta ccccgttcga gccggaatgg gaaggctacg ttaccctcga gttttccaac
     8941 accacccccc tgcccgccaa aatctacgca ggcgaaggcg tggcgcaagt cctcttcttc
     9001 gagagcgacg aagtgtgtga aacttcatac aaagaccgca acggcaaata catgggacaa
     9061 accggcgtaa ccctgcccaa agcctgaaat acaaaatgcc gtctgaaccc ttatcgtcgg
     9121 gtttcagacg gcatctacct atttatccgt ggttagaact gaaccgtccc attgatgctg
     9181 atgctgattt cctccacacc cggtgcggct gaatccgtac ccttcatatt gacgcttgcc
     9241 gccatcggca tcgcgcgcag catttttgcc cgaacagccc catcgcccgc aatatggctg
     9301 ccgatttgcc caaaattcaa tttgacgatt ttataaccgg acgcacccag aacgcccgcc
     9361 agtttttcgg cacgcgcctt gaaacgcaaa acggcatcct tgctgacctg atcgatgacc
     9421 tcgtttcggc gttcgcgcga cacgctgaaa tcggtatctt caagcgaagc atccgtctga
     9481 acatcagcaa taaaacggtt taaagcatca aaatccctgc cctccgcctt aaattccgca
     9541 cgctcctccc agcctgtttg aatgcgtctg ccgttggtat attgatagcg cggcatcgca
     9601 ctgcgcgata ccaattcggt tttaaagcta ccatttttcg attttctggt aaaattgttg
     9661 aattttttaa caaactcggc attgacggca tttttgtccc gtccttccgc cgccacctgg
     9721 aaacgcgcgg acattgtatc ctgagccacc tcgatacccg ccgattcgga aaattcgaca
     9781 atattgtaat tcaatgcctc agccgccgcc ggaaaagata ccgccagcag ggaagccgcc
     9841 aaaacagaac gcagcatatc cgtatcctta cttgaaaaac cgccaatcta acatataggt
     9901 acgctaaatt gcgttagcat aagacaacaa tttaaatatt cgatcggtct gccttataat
     9961 aaccgtcttt ccgatactga atcatattat gtccgaaccg ttgcacgtcc tcgtcatccc
    10021 cttatggtat ccacgatccg aacaggatgt ggacggaata tttttccaaa atcaagcaca
    10081 ggccctccaa cgcaaaggca tcaaaaccgc catcctcgcc cccatgttcc gttatctgcg
    10141 gaaagaaaca gcaagcatcc tgaccggtcc ttacggtttt gcccgatacc ggcaaagcgg
    10201 tttggacatc tacgcatggc gcagcatgta tttcttcccc gctttccgtt tatcgacatc
    10261 gaccgcatcc gctgggtgcg cgccggtttg aaagccttca aacactacat ccgcgaaaac
    10321 ggactgcccg acctgattca cgcccactgc atgaactatg caggcatact tgcccaaaaa
    10381 atttccgaaa aatacggcat tccctacgtt ttaaccgaac acagcagcac catcacgcgc
    10441 ggtctgatcc gtcaccacca atggcagccg atggaaaaag cagcggcgcc cgcctccgca
    10501 cgtctcgccg tcagccgcca tttcgcacac gtcctgcaac acaaatacgg ctgcgaatgg
    10561 cagtacctcc ccaacatacc gggtggaata ttcaagcaaa catttgaata aaaagaaaaa
    10621 aataacaaac cgcatttcgt gttctgcacc gtctcgcacc ttcgccgcct caaaggacac
    10681 gatgtcctgc tcactgcttt tgcccgggcg ttggcacaat gcccgcaact gcgcctgaac
    10741 atcggcggca gcggacagga agaacagcgg ctgaaacagc aggcggcaga cttgggcatc
    10801 gcccatgccg ttacattttt aggcgcactg caacccgaag caatcttgga tttgatgagg
    10861 aacagcgacg ccttcatcct cgccagccgc accgaaacct tcggcgtggt ctatatcgaa
    10921 gccctctcgc aagggttgcc cgtcatcgcc atacattgcg gcggcgcgga atccatcgtt
    10981 tcagacggca acggatacct cgtttccgtt gacgatgccg atgtcctagc ctttctcaaa
    11041 atatatgaac accatgccga tttttccgcc gaacagctca gaaccgactg cctcgcaaca
    11101 ttcggcgaag atgccgttat cggcagactg attgccgtat tccgtcaggc ggcgcggaac
    11161 acggcgcgaa aacgcccgaa aaatagatta aaatgacgca aaacaacctt cggattcaaa
    11221 tatgacccat accgtccacc tgcacttgga agaaaccgac aacccgacgc tgcaacgcct
    11281 gtgcggttct ttcgacagca acctcgattc gctcgccaaa gcactcgaca tccatatcag
    11341 ccgccgtttc gagcatttta ccttcaacgg tgcatttgca cacgccggca aacgcgcact
    11401 gctcaaactc ttggaaacgg cgcagacgcg cgacctaaac gacagcatca tccgccttgc
    11461 cgccgtcgaa acccaaaccg aagatgccgg gcatcaggaa aaaaatcacg aacacgccta
    11521 ttatttccga accaagcgcg gcagcatagg cggcagaacg ccacggcaga acggctatat
    11581 ccgcgccctg ctcgaccacg acatcgtatt cggcctcggg ccggcaggta cgggcaaaac
    11641 ctatcttgcc gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat
    11701 tttagtgcgg cctgccgtcg aagccggtga gaaactgggc ttcctgcccg gagacctgac
    11761 ccaaaaagtc gatccctacc tccgtccgct ttatgatgcc ctctatgacc tgatgggctt
    11821 tgaccgtgta accaagctga ttgaaaaagg cctgattgaa atcaccccgc tcgcctatat
    11881 gcgcggcagg acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc
    11941 cgaacaaatg aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg
    12001 cgacaccagc caaatcgacc tgcccaaaaa catcaaatcg ggattgaaag acgcacgtga
    12061 gaaactgcac ggcgtggcag gactgtattt ccataccttc accggcgaag acgttgtccg
    12121 gcatcctctg gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacactc
    12181 caatgccgtc tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga gacggaagcc
    12241 aagctccgta tccgtttctt tatccgtcca accgatacat caatcgaatt ttctgcatcg
    12301 gattgcctca tctccgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt
    12361 ctgccgcgcc acgccttcat tccgatcgct tgcctcaaac gtcttctgaa aaggcgtaac
    12421 gctgcataca tacatggtgg aatttgaacc gaaaaggtcg attttgacgg tattgccgcc
    12481 gctttccaat acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgctgat
    12541 tctgtcggca aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt
    12601 catcaatttt tccttgttgc ttgaaccctg cccacccgga catccttttt ccgggcggca
    12661 gggtcagact ttatttgcag ggatgaagcc ccttacaaat cagaataata catgaatcgg
    12721 cactttatgc ttcatcctat gtgttgaaac ttcattgctc cttgtctgtt gaccgttatt
    12781 cccaatccgt tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg
    12841 cctgtccttc gtttttttcg tgccgggcgg cggcctcgtt tttggtcatt tccgtccgct
    12901 gttccgccga tgccgtctga aacgccgtcc accactcggc aagtgcgcgg tccgcattgc
    12961 cggtttcggc gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca
    13021 gtttgtgcgg acgtgcgcct ttgcggtttt caaactgcgg ctggaacatc caaatttcac
    13081 gcatcgtggc ggaaaagcgt tgcggcacgc cccaaccgcg ttcgacggtt tcgcgcatcg
    13141 tattgattgc atcggacagg gcgggcgtag gtttcaaacc ctgttgcaga ttgcttttcc
    13201 aatggcggtc caactctggc cacatcaggg ccgccagtac gaaaccgacc gaaaccgatt
    13261 tgtcggcacg cagccgctca tcggtatttt tcagggcaag cgccgtcatt tttccggcga
    13321 tgccgtctga aacgcgcaaa gcattgagca gcggatggat gtccggtatg tcaaatccgt
    13381 tcaaacgttt cagacactcg cgcgcgtgcc ctgaaaacag cagtttcatg atttcgtcga
    13441 acagccttgc cacaggttcg tgcttcagac ggcatatcga ttcggcaatc ggtgcggcgg
    13501 tttcttccga cagctcaaag cccaatttgc ccgacaggcg gatggcgcgc aaaatcctga
    13561 cagggtcttc ctgatagcgt tcggcggcat taccaatcat aaccagcctg cgggcggcaa
    13621 catcggcaat cccgttgtgg aaatccaaaa tctcttcttt ttcaggatcg taatacaagg
    13681 cattgcaggt aaaatcgcgc cgcatcgcat cttcttcgat gctgccgtag gtgttgtcct
    13741 tcatgattct gccgtgcgcg ttctgatgaa ccttcgcacc gccgcgaaac gtcgttactt
    13801 cgataatctc cgcaccgttc atcacatgga caatctgaaa acgcctgccg atgatgcggc
    13861 tgcggcggaa gagtttgtgc acctgttcgg gcgtggcatc agttgcgaca tcgaaatctt
    13921 tgggttcgat gccgagcagc aggtctctga ccgcaccgcc gaccacataa gcctgaaacc
    13981 ccgccccttt caggcggcgt atgacgtttt cggcggcaaa gctcaacatt tcggcactga
    14041 tgttgtgtct ttcggcagga atgaccgttt tactttccgc ttttttactg ctccgaccgg
    14101 aaggcagcat cttattcaac cattttttca gcataaatat tctgacccga agagcggcat
    14161 ctccacgggc aaccgtgttc agactgcatc aggcggtacg attccacaag atgccgaaaa
    14221 ccataagcaa aaggcgtaga aactacgccg tccgaatcac gccgccctcg cggcggcaac
    14281 gcgtcattat aacagattgc cccttaaaaa atcagaccct gcttttgtgg cggagtctga
    14341 aatttgatgc accttatata ttgcgtccct aagaagggat gattaacaaa aattaacgcc
    14401 ctttactttc ttttccgatt gggaacattc ccgtcgtaca aacaagcaaa tgaaggaaca
    14461 gaaatcacat tcaaacggcg ttccgccgcc tctacgccgc ttcccagtag tcgatataaa
    14521 gctgcaattc gaggttgtcg cgccattcgt tggcaacggg gcggtacacc gtgcggatgt
    14581 attcgggaat gtcttcgctg caacgccaga acatcgcttc aaattcgcag ccgtcttttt
    14641 gcaaccacgc ttttttgtgt ttgccttccg cgcccaaagg ctgctggcga acgacgtgga
    14701 actcgtcggt aaagctcggc ggcgcaaagc cctgccccca aacgtggcag gcgaggtttt
    14761 gcgcctgttc caacgtgatg tcgcaggcgg gcaggctgcc gtcagtaata taggtttgcg
    14821 acaaatcgtc ttcgcacacc atctcacgca cggcttcttc aaaggccgtc tgaaacgcgg
    14881 gaatattgtg ttcaagtatg cttaaacccg ccgccatcgc gtgtccgccg aatttcaaaa
    14941 tcaaatcggg atggcgtttg gacaccaagt ccaaagcatc gcgcaagtgc agattgggaa
    15001 tggagcgtcc cgaaccgcgc acttcgccgt tgtccgccgg cgcaaacacg atggtcggac
    15061 ggtaaaaacg gtctttgagg cggctggcaa caatgccgac cacgccttga tggaagtcgt
    15121 cgcgatacgc caccaaagtc gtctgacctg aaggcagggt ttcgggaaag gcgttcaacg
    15181 cgtctcgcag catagactgc tcgatttcgc ggcgttcgat attgaggttg tttaaccgag
    15241 ccgccagttc ctgcgcttcg gaatcatctc gcgccaacag gcaggcgatg ccgaccgaca
    15301 tatcgtccag ccgtccggcg gcgttgatgc gcgggcccaa cgcaaagccc atatcaaacg
    15361 gctgagcctt gcgccaatcg cgccgcgcca cttcaaacaa ggcgcggatg ccggggcgca
    15421 ttttgcccga acgcatccgt ttcaaacctt gcgacacgag gatgcggttg ttgtggtcga
    15481 gggggacgac atcggcgacg gtaccgagtg cgaccaaatc caaaaggccg cccagattcg
    15541 gctctttgat gccgtctgaa aaataattgc ggcggcgcag ttcggcacgc aacgccgtca
    15601 acacataaaa aatcacgccc acgcccgcca agcttttgct tggaaaaccg cagccttttt
    15661 ggttcggatt gacgatgatg cagtcgggta cggtatcggc tggcaaatgg tggtcggtaa
    15721 cgatgacatc caaacccaga gcctgcgcgc gcgccacgcc tgcaatactg gcaatgccgt
    15781 tatccaccgt aatcagcaaa tccacgcctt gcgcggcggc gatttcggcg agttcgggcg
    15841 ttaagccgta gccgtgctca aagcggttgg gcacgaggaa atccactttc gcgcccatcg
    15901 ccgccaaacc gtccaaaccg acggcacacg cggtcgcgcc gtcggcatca tagtcggcga
    15961 caatcaggat tttttccttg cgttcaaccg catccgccaa acggccggcg gcggcttcgc
    16021 aattcgtcag cgattgataa ggcaggaggg aagcgagttt gtcgtccaac tcggcaggac
    16081 tttgcacacc gcgcgaggca caaagccggg cgattaaagg atcggcgccg gcggcgagca
    16141 aatgattgaa aacgtcggta ttgacggggc gggtttggat tttgactgac atagcgcggg
    16201 gttttacagt gcgggtgtgc ttgaaatgcc gtctgaaggc ttttcagacg gcattgccgt
    16261 tcatttgtct gaggaattcg cggctgcaat cagagccata ggcaagacca gcaccgcggc
    16321 cgccgcatcc aatatcaaca aggggggcgt atataagata tttccaaaca gcttggattt
    16381 gtcggtatgt ttttcagtaa ccgtataata aatatcggcg ggcacacttt gctcaaaatg
    16441 ataatcggcg ttcagttttt gcggcgtggc gtagtatttg cctttggcgg atacgcagcg
    16501 cgtgtaaatg gtccgattgt cgagtttgac cgctttaaac tcaagctgtt tcagcttggc
    16561 gatgtcgtca ggtctgccgg tatcatagcg caggcaaaga cctccggtac tgaaattctg
    16621 gctgccgggc gcttcgaatt tgaccggcag ggcttggtgg cgggcatagc tcggggtatc
    16681 ctcaactatt tggaagggct tgtccaaccc ggccttcaaa aggcccgtca gcttcgccga
    16741 atcttcggga ttgacggcga accagtattt cccgcccatc atcaccaggc tgcccttttc
    16801 caattgggca ttgtcttcgg caaccacacc gaaggcgcgg atttggtctt tgtcaacgtg
    16861 tttgcgggta attgtttggc tgaccgggtt gttcatcccc cgcaacatca tcgtacagcc
    16921 gttcagtatc aaggttgccg ccaaaacggc ggcggtcgtt ttacggaaca acattttatc
    16981 ttccaatcta aataacggcg gatgccgtct gaaaatatgc ctaaatttcc gcgcccccgt
    17041 tttccatcac ggttttcaat tcttgaaaca gggcgcggaa gtttttcggc ggtttgtcct
    17101 gttcctgttc ttttttggtg ttgcggacga gcgtcctcag cctgcccgcg tcggcgtttg
    17161 gaaaatcgga cataaactgc gtcagcgcgc cctcgtctgc caacaaccgt acacgcgcct
    17221 gttccacgcg ttgcaaaaag gcgttgtgcg ccgcatcgtc gccgcgcagc ttggcaagga
    17281 acgcctcgat gggcgcggga tcggtatcgc gcatcagacg tccgataaat tgcgcctgac
    17341 gtttgagcgc gccgttggac gtaatttttt tataggtaac gacggcctcg tacaaatctt
    17401 catccaaacc gatttttttc agcgtgtcgt ttgagagcct ggtcagttcc atacccaaat
    17461 cctgcaaacc gttcatctgc tttttcattt gggttttgct gatccactcg tcttcttgtt
    17521 caaacatctt gattctcgaa ttttttccaa tagcgtattt taacagaatg ccgccgcgtt
    17581 cagacggctt tttgcaccgc acgccctgca agcggcacgc ttttgcccct gccgcccgaa
    17641 acggttaaaa tgccgcaacg ctccaccaag aaagattgtt atgctgttca accacaccgc
    17701 ttccgaactc ctcgacctct gccgccgcac gctcgacttg gcaaaagcag cgggcgcgac
    17761 cgccgccgaa gccgacttca gcgaatcgct cggacaaagc gtcagcgtgc gcttgggcga
    17821 aatcgaacaa atcgagttcc agcaggacaa atcgctggac attaccgttt acgtcggcaa
    17881 acgcaaaggc cgcgccggca ccgccgactt ctccgaacaa gccctgcgcg acaccgtccg
    17941 cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgaccc
    18001 cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
    18061 ggaagccgcc gtcggcttgg caaaacaatg cgaacaggca gcgttggaca cggacagccg
    18121 catcgaaaac tccgaaggcg cggcggtgca aaccggacac taccaatacg tttacggcaa
    18181 cacccacggt tttgccgcac accggcaaag cacgcaccac agcatttcct gcagcgtcgt
    18241 tgccgccgac gaaaacggaa tgcagcgcga ctactggtac gattccgcct gccgccatcc
    18301 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
    18361 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
    18421 cgggctgatc ggacacctcg tcggcacact ctccggcggc gcgctctacc gccaaagcag
    18481 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
    18541 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggtg ttgccaccgc
    18601 gccgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcggca gttacagcgc
    18661 gcgcaaactc ggaatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
    18721 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg ggcacgggat tgctggtaac
    18781 cgaattgatg ggacagggtg cgaacaccat caccggcgac tattcgcgcg gcgcggcggg
    18841 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg ttgccggacg
    18901 cttgcaggat atgtaccgcg acatcgtcgg cgtggcggat gacgctttgc ggcgttcgtc
    18961 caacaaaatc ggttcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
    19021 ccgatgccgt ctgaagacga aggcggcgcg ttttgccccg tgcctgcccg tttgccgttt
    19081 tatgccgcac cgccttttac cggcgacatt gcacattttt ttcatcagca ctgattttcc
    19141 cttatccgtc aaaatactaa gccacttgcc cacaacaggg tacacacaag ttcaggataa
    19201 ggcgggcggg gattggggac ttttccgcca tcggcaaaac ggcagacaaa aacggcggga
    19261 cattgcctgt cccgccgttt tcaaatatac atttgaccgt acatcaggaa ttaagctgcc
    19321 tgaatgttgg cggcctgttt gcctttaggg ccggtggtta cgtcgaaaga gacgcgttgg
    19381 ccttctttca gggttttgaa accttccata ttgatcgctg agaagtgtgc gaacaaatct
    19441 tcgccgcctt cgtcaggagt gatgaaacca aaacctttag cgtcgttaaa ccattttacg
    19501 ataccggttg ccattagaaa cttcctatac ttaaaaatta acaaaatcag caaaataagg
    19561 catacagcaa acttaaacgt tcagcgtttc tacccagctt tatatacttg gtcgagtgcc
    19621 gacttttgct taaccgtctt tttacatttg ttaagacaaa acgtcaagct atgttttcaa
    19681 aaaagtggga aaataggcga aagtaccgaa aatacaacaa aaatgccgtc tgaacgcggc
    19741 tttcaaatgg gacacgctat gaccgcgcaa caccaatccg acacgctttt gcaccgcctg
    19801 aacaccctgc cgccgaaacg ttacggcgta tttttattga acgacgatta caccacgatg
    19861 gaatttgtcg ttgaaatcct gaccgaaatc tttatgctcg ggcaggaaca ggcggtagcg
    19921 gtaatgctct cggttcatca cgaaggcaaa ggcctgtgcg gcacttacac gcgcgatatc
    19981 gcccaaacca aacagcaaca agtcatgcag cgggcaaaag ccgaaggaca tccgctgcaa
    20041 tgtattgtcg aggagattta atatgcttgc ccctgaattg gaacagattt tgcagcagct
    20101 ttaccgcgag gcgcgtaagg ctcattatga atttatcagc ctcgagcatc tgcttttggt
    20161 actcatcgaa gaagatgccg ccgtgccgaa cgtcttaaaa ctctgcggtg cggatttaaa
    20221 agcggtgtcc gaacagctcg ccgccagcgt tgccgaaaac acccccctga ttcccgacca
    20281 ccttttagac acggtcgaaa cccggcccac gctcggcttc caacgcgtca tccagcgggc
    20341 gatggtgcat acccaatcgg caggcaaagg cttggccgag cctttggacg ttttggtcgc
    20401 gctgatgagc gaaaccgaca gccacgccgt ctatttcctc aagctgcaat cggttacgcg
    20461 ttttgaagtt ttgcgctgta ttgcccacgg ctctcccgat gaagatgaag acgatggcaa
    20521 ctatttttca gacggcatgg acgacgataa tgagaaccgc accaaaccgg gcaaaaaccc
    20581 tttatcggcg tacaccgtca acctcaacgc cgaagtcaaa gccggccgta tcgatccttt
    20641 gattggtcgc aaacacgaaa tggaacggct ggtgcaaatc ctgtgccgcc gccgcaaaaa
    20701 caatccgctt ttggtcggcg aagcaggcgt gggcaaaacc gcgctggcgg acggtttggc
    20761 acatcaaatc gtcaacgacg acattcccga cgcgctcaaa gaggccgaag tgtacgcgct
    20821 ggatatgggt tcgcttttgg cgggcacaaa ataccgcggc gactttgaag cgcgggtcaa
    20881 atccgtcttg aaacagctcg aaaaaatccc gcacgccatt ttattcatcg acgaaatcca
    20941 caccatcatc ggcgcgggca gcaccggcgg cggcacaatg gacgcgtcca acctgctcaa
    21001 acccgcattg gcgaaaggtt cattgcgctg tatcggcgca accacttacg acgaataccg
    21061 tactattttt gacaaagacc acgccctaag ccgccgcttc cagaaaatcg acgtggtcga
    21121 acccaccgtt gccgaaaccg ttcaaatcct gcgcggcttg aaaccgatgt tcgaagcctt
    21181 ccaccaagtc cgctacacgc aaggcgcact cgaagccgcc gccgaactct ccgcccgcta
    21241 catcaacggg cgcttcctgc ccgacaaagc catcgacgta atggacgaag caggcgcggc
    21301 gcaacggatt ctgcccaaat ccaaacagaa aaaagtcatc ggcaaagcgc aaatcgaaac
    21361 cgtcatcgcc aaagtcgcgc ggattcccga aaagaccgtg tcgcacgacg acaaacaagt
    21421 gttgcaattc ctcggccgcg atttgaacaa tatggtttac gggcaggaag acgccatcga
    21481 cgcgctggtt tccgccgtca aaatgtcgcg ttcgggcttg gggctgcccg acaaaccgat
    21541 aggcagcttc ctcttctccg gccctaccgg cgtcggcaaa accgaagtcg ccaaacagct
    21601 tgcctactca atgggcgtac cgctgcaacg ctttgatatg tccgaataca tggaacgcca
    21661 cgccgtatcg cgactcatcg gcgcgccacc gggctacgtc ggctttgaac aaggcggcct
    21721 tttgaccgaa gccgtcaaca agcagcctca ttgcgtgttg ctcttggacg aaatcgaaaa
    21781 agcccatcca gatattttca acgtcctcct gcaagtcatg gacgcaggca aactgaccga
    21841 taacaacggt aagagcgccg atttccgcaa cgtcatcctg atcatgacca ccaacgcagg
    21901 cgcagaaagc ctcagccgac ccagcctagg ctttaccgcc aaacgcgagc gcggcgacga
    21961 aatgcaggca atcaacaaac tcttcacgcc cgagttccgc aaccgcttgg acgcgatcat
    22021 cccgtttgca cctttgtccg aacccatcat cgtcaaagtc gtggacaaat tcctgctcca
    22081 gctcgaacac cggctcctcg acaaaaaagt cgaagccgaa ttcacaccgg cattgcgtaa
    22141 atacttggca gaaaaaggtt tcgacccgca aatgggcgcg cgcccgatgc accgcctgat
    22201 tcaggaaaaa atccgcaaac cgctcgccga cgaactcctg ttcggcaaac tcgccgacgg
    22261 cggctttgtg cggatagact gggatgcggc gaaagaagaa gccgtattga agtttaagaa
    22321 aagcaaggtc aaaataaaaa ccgcctccgc ataaaatcaa aaatgccgtc tgaaaggttc
    22381 agacggcatt ttgtcaaata tcggccccta ttccggcaaa cttctccaca aacgccgcga
    22441 ttttttggaa gacgctttgt ttcttcgtca gatattgcgg gttgagcgga ctcattttcg
    22501 gcagggtttc ggtcagttcc gtgccgtttt cgctggcata gccgcgtttg agcgagccgg
    22561 tcaaatagcg tttggcggcg gtttcgttca gaccttcggc ggcaatcaat cctgccgctt
    22621 cgtgccgcat cacctcttgc gcgtaggtgt aaaaggtttc cagtatggcg ggaacgtcgg
    22681 gcactttatc caaatccgta tcgttgatga aatccacaat caggccctct ttggcgcggt
    22741 ggccgatgct ggcgcggatg atgcggcgga tttcttccac cagctccgct ttgcctttga
    22801 tttttttgtg atgctcgaaa accagttgca ggatgtaatc cagattgatt tcctgcgatt
    22861 tgagcaaatc cacctcaaaa accacatcgt cccagtcgat ttttgactgt tctttctgtt
    22921 cgcctgcttt ttggcggcgc agccagtctc ggatgtcgtt ataggcggaa cggtagtcct
    22981 gcaccgccct ttcagacggc atcggcactt tccgcatttc ctgcacgtct tcgtcgctca
    23041 ggtagtattt ttcctgaaac gccttcatcg catcttcgtc cgccgcgtcc acattctgca
    23101 attcgcgcag cgcggcaaat tcatcgtagt tctgcaatac gttttccgcc cgcaggtatt
    23161 cgccgaagag tttggcaaaa tcttttttgt ctttttccgt ttcgattttg tcgggatcgg
    23221 ggaaacgctc gtgcaattct tttgctacat ccagataacc gcgccgtgct tcgccggtct
    23281 ggctgtcggt atagccgttc atgtattctt cgtagctttt ttccagcact atatttttgg
    23341 tgtttttgtc gccgaagagc gtgatcgcat caatggttgc ctgctccaag tcgcggaagc
    23401 agacaatgtt gccgaaggtt ttggtagcat cgtaaatgcg gttggtgcga gaaaacgcct
    23461 gcatcaggcc gtgatagcgc aggtttttat cgacgaacag cgtattcagc gtcggtgcgt
    23521 caaaacccgt caaaaacatg ccgaccacaa tcagcaagtc cacttcccgg tttttcaccc
    23581 gttttgccaa atcgcggtag tagttctgaa aggctttgct gtccgtgccg aaattggttt
    23641 tgaaacaggc gttgtaatcg ttgatggcag cctgcaaaaa ttctttcgcg ctgctgtcca
    23701 tcgcttccgg ttcaaaggtc tcgtcaacaa tttcaccgac ggcgttttgc tcttcgttgg
    23761 cggcaaagga aaaaatggtg gctgttttca acgggtgcag gctgcctgcc tgttgtgttt
    23821 tgaacgcttc gtaatagcac ttcgccgcat ccacgctgct gacggcaaac atggcgttga
    23881 agcctttgcc gcccgcattc aagcggtgcg ttttctgcct gaaccggctc aggatatatt
    23941 gcgtgatttc gcggatgcgt tcggggtgca gcagggcttg gcggttttcg gcggcactca
    24001 gttccttctc gtcctgttcc gcttccacgg ctttgaactg cgggcgcaca tcgttgtaat
    24061 ccactttgaa tttcaatact ttttcatcgc ggatggcatc ggtaatcaca taagaatgca
    24121 gctcccgccc gaacacgccc gccgtggttt ccgcgcccaa agcgttttcg ggaaaaatcg
    24181 gcgtgccggt aaagccgaac tggcagaatt ttttaaattt ctttttcagg tttttttgcg
    24241 cttcgccgaa ttgcgagcgg tggcattcgt cgaaaataaa tacaacctgc cggtgataaa
    24301 ccggcagatt atcttcactt ttcatcaggt tgttcagctt ttggatggtg gtaacgatga
    24361 ttttgttgcc gtctttttcc aaattgcgtt tcaagcccgc cgtactttcc gagccgttta
    24421 cgctgtcggg cgaaaaacgt tggtattcct tcatcgtctg atagtccaaa tccttcctgt
    24481 ccaccacgaa gaaaaccttg tcgataaacg ccgattccgt cgccagacgc gccgccttaa
    24541 agccggtcag cgttttgccg ctgcccgtgg tatgccaaac atagccgccg ctttccggcc
    24601 cgctccaatt cttcgcctgc gccgagctgt tgattttcca caaaatgcgt tcggcggcgg
    24661 caatctggta cggccgcata atcagcagcg tatcattcgc atcgaacacg ctgtaatgca
    24721 gcaaaacgct cagcaatacg cttttctgca ggaacgtggc ggtaaagtct ttcaaatcct
    24781 taatcggatg attgtccgac cgcgcccaat tcatcgtgaa atcgaagctg tttttgtcgc
    24841 gcttggtggt gttggcgaaa tagcgcgtgt ccgtgccgtt ggaaatcacg aagatttgca
    24901 ggaatttgaa cagcgaattt tcgctgttga agctctcttt gctgtaacgg tgcacctgat
    24961 tgaatgcctc gcgcaccgcc acaccgcgct ttttcaattc aatctgcacc agcggcaggc
    25021 cgtttaccaa cacggtaacg tcataacggt ttgcatgcgt gcccgtctgc tcaaactggt
    25081 tgataagctg cacatggttg cgggcaaggt ttttcttgtc cagcagataa atgtttttca
    25141 gacggccgtc atcaaaagcg aaatcgtaaa tatggtcgtc gtggattttg cgggttttat
    25201 cggtaatgtt ttcagccggc ctgtccagat attccgtcaa aaaccgcgcc cattcgccgt
    25261 ctgaaaacgc cacatcgttc agccgctgca actgggcgcg caggttttcc agcagcctgc
    25321 tttggctgtt caaatccttg cggtattcgt acccctgatt ctgcaaatcg gcgattaact
    25381 ccgcttccaa ccggttttcc gattggtagc tgcccgactg ttcgattttt tcatattggt
    25441 cgagcacgat gaaattcggc gtttcggcga tgggtttggt ttcgaggttc atgggtcgtc
    25501 ctttgcggtg tcgggtttta tagtgaatta aatttaaacc ggtactgcgt tggctcgcct
    25561 tagctcaaag agaacgattc cctaaggcgc ccaagcacca ggcgaatcgg ttccgtacta
    25621 tttgtactgt ctgcggctcg ccgccttgtc ctgatttttg ttaattcact ataaacggtt
    25681 tgatgttcgg attgggctgc ctgaaaaagt gcaggctgct ttttcaggca gccttgggga
    25741 aggcaagcag ctgttcgcgg taatattcgt attgtttgcg gcgcagggcg atttcgtgcg
    25801 gcaggccttc gctgacggag tgggtcaggg tatcgaattt gcctaggatg gcgacgattt
    25861 tttcctgttc ggggagcggg gggatgggga ttttatattg cataatcgct gctttgctgc
    25921 cacgcggcat ttttgcacct ttggcgtgct tcatattaaa agcaaaaaat ttatcatcgg
    25981 ccaatacctg atacagatat tttggattaa ccttttcatc tgttacccgg ataaccaaaa
    26041 catcaccgtt tgttccgccc gtgcagtctg cctgccagat ttttttcaaa tacggacgga
    26101 tatttccaat caaaatgtca ttgacaatat attctgtcat ttttccttcg cttggaacat
    26161 aaccggataa ctttttacct tctctgtttt gtaagagatt atccacgcca acgtaattat
    26221 gttcgttcag tttatccgaa caaatacggt tttttgaata ttcggcaacc tcccccaacg
    26281 tcttccaaac cacatttttc agacggcatt gatagccgtc agctatcccc cccccgattt
    26341 gattgtcaaa atctaaaagt aagtcgcggt aataccggta ttggcgtttg cgcagggcta
    26401 attccgcttc cagcgtagct tccagcgtag cttccagctc ggtgaatttg tcaagtattt
    26461 ttacaatttt ttgttgggtt tccaatgatg ggatggggat tttatagtta tctgtatctg
    26521 gtgttgctat ttgtggcatt tgcatttttg atccgatatt acgaaaatag ttttcttgtg
    26581 tttttaagaa ataataaaca tattttatat aaatatgttt attaactgaa tgataagacc
    26641 acatttcatt tttatgcgaa aatggtttat cgtaatattc gaattcaata atacctcttg
    26701 atttaacgat aatagaaggc tctctatgga tatccttatc aggaacatca tcaaagtcaa
    26761 ctaatgcaaa agtttttcct cctgcaaaaa tctttaacgg cgcattatct ttatgcattt
    26821 ctttcatttg tccagcagtt atcttagttc cttttgtacg aactaaaacc tcccccaatg
    26881 gcttccactc caccggtgcc gtctgaatca tctcaatcaa ttttttcgct ttgctttgca
    26941 tatccatggt tgttttccgt ttccttcccg ctgcggcggg atgtggggcg gttgcggggc
    27001 gggtttcggc tcactctgtc cgtttgccgt tgtttctgcg ggctgaaacc cgtcctgctt
    27061 tcaggctgcc tgaaatcgtg tgcaggctgc tttttcaggt agcctgaatg cggttatttt
    27121 ttgccgtttt gatgctgttc ggttgcggca agccttttct gttcttcgtt ctcaagctgt
    27181 ttgatgcttt tctcgggtac gggctggttt tcaggcataa tgccgcccag ttcttcaatg
    27241 gtttggcgca ctttttgtcc gacttcgaaa tgcacccggt ttgcctgtgt tttgccttgg
    27301 atgtttttgc ggcgtagttt ttcttcggtt tgcgtggcgc gaaacagatt ggcagccggt
    27361 tcgctggcgt tcatgtggtc taggattctc tggcttttct tcaagccttt gcggctgtgt
    27421 atgccctgct tgtccagtcc tccgtatagc ccccgatagc cgtggttttg gaagacggcg
    27481 tactcaacgg gtttttctac gcctgcatcc ttggctgcgg cggcaaggtc ggtattgtgt
    27541 tcgcgcagct gcctgcgaag cagcaggcgt tgtttgtctt cgcccaaact cctgaatgct
    27601 gcctcgtctt gcagctcttg gcggcgggct tgcacggcaa agtaggtttg ccctgcggca
    27661 atgacgcttt tggacggatc gccgttttgc acaatcaggt agcaggcata gcgggaaagg
    27721 cggtaatccg ccacttcgcg ctgcccgcct ttcccaaggg ctatcatttt gttggtttca
    27781 acaaaatgat gatttttgtc atttcctgat gtttcgcaag cggttatggc tttatcaatg
    27841 gcccgttgga aattgcgcca ttcggaatat tccaagattt gctgcaaggt gcgcgccgac
    27901 caatattcat tgcctgtttc gtcgatgtgt ttggcgtttt caaaagcgtt attttctgtt
    27961 gtcatgtatt ttccccgaaa atttcttgat ttgaacttgt aaggattgct tacaggttgc
    28021 cgcccgtatt ttcagacggc ctctttccca ttcagttgtt gcaaaatatg caacaactgc
    28081 ctaggtttca atctctgcaa tcacttcgtc aatttcacgc cgcagccgtt cgattttggc
    28141 gacggtttcg ctgatttcgg cgttgagctg tctgatgtcg atgacctcgc gggtgtcttc
    28201 ggcttcgaca tagctgctga cggcgaggtt gtagccgttg tctttgacgg tttgctgggc
    28261 ggcgttttgg gcgatatgcg gcacatcggc tttgtcggcg aagagtttga cgatttcggc
    28321 aatgtgttct tcggttaaga cgttgttgtt ggtttctttt ttaaagaagc cgcctgcatc
    28381 gatgaattgg atgtcggtat tgtctttgtg tttggacaaa accaggatat tgacggcgat
    28441 gcaggtgccg taaaagagat tgggcgcaag ggcaatcacg gtttccacat agttgccctc
    28501 caccagatat tggcggattt tctgctctgc gccgccgcga tagaaaatgc cggggaatga
    28561 gacgatagcg gcgcggcctc tgccggaaag gtagttcagt gcgtgcagga tgaaggcaaa
    28621 atcggctttg gatttcggtg cgagtacgcc tgcgggggca aagcggtcgt cgttgatcaa
    28681 ggtggggtcg tcgctgccta tccagtcgat ggaatagggc ggattggaga cgacggcatc
    28741 aaagggtttg ctgtctttga gtttggggtt ggtcagcgtg tcgcccaatt cgatgtggaa
    28801 tttgttgtaa ttgacgttgt gcagaaacat attcatgcgg gcgaggttgt aggtggtgtg
    28861 gttgatttcc tgcccgaaga agccttcttc gatgatgtgt tcgtcaaact gttttttcgc
    28921 ctgcaagagc aggctgcccg agccgcaggc ggggtcgtag attttgttga ctttctcctg
    28981 cccgtgcacc gccagccgcg caatcagctt ggagacgctt tgcggggtga aaaattcgcc
    29041 gccggatttg cctgcgttgg cggcgtagtt ggaaatcagg tattcgtagg catcaccgaa
    29101 aaggtcgatg cggtggtctt caaaattgcc gaaatcgagt tccgccacgc ctttaaggac
    29161 ggcggcaagg cgtttgtttt tgtcggcaac ggtgctgccg agccggctgc tggtggtgtc
    29221 gaagtcgtca aacaagcctt tgatgccttg ttcggacggg tagccggagg cggagctttc
    29281 aatcgcggta aagatttctt tcagcttggt gttgagctct tcgttttgat gggcttcggc
    29341 ggcaatattg caaaaaagct ggccggggta gatgaaatag cctttgactt tgacggcatc
    29401 gtctttgatt tcgggcgtga tgatgctgtc cggcatagcg gcgtaatcaa tgctgctgtc
    29461 gccggcctgc atatagtcgg tgaagttttc gctgataaag cggtagaaaa gtgtgccgag
    29521 aacgtattgt ttaaagtccc agccatccac cgcgccgcgt acttcgtcgg cgattttcca
    29581 aatttggcgg tgcagttggg cgcgttgttg catttcggtc atcatcgaaa tccatatata
    29641 aagttaaaca aatcaaaatc gcctgatatt ttcagacgat ttttttacgg gcattcaaat
    29701 taagccaaat tttccagcat ccatttgacg tagcggttga cacctgcttt gacatcgaaa
    29761 aattcttcct tatatccggc ttcgcgcaat ttggtgatgt cggcttgggt gaagccctgg
    29821 tatttgcctt tgagcgcgtc gggaaagggg atatagcgga taagttcttc ttctaccaac
    29881 tctttcaagc tcaattcaga tttgccttcg gcggcgcggc aggcgttgac ggtggcggcg
    29941 gcgagttcgt tgaactgttg gctgcggccg gtgccgaggt tgtagatgcc ggaaagttcg
    30001 ggatggtcga agaagtagag gttgattttg gcgacgtctt cgacgctgac gaagtcgcgg
    30061 gtttgttcgc cgttgccgta gccgtcgttg ctgccgaaca ggttgacgta accgtgttcg
    30121 cggtattggt ggaaatggtg gaaggcgacg gatgccatgc ggcctttgtg ttgttcgtgt
    30181 tgtccgtaaa cgttgaagta gcggaagccg acgacttggg cggtgagacc ttctttcatg
    30241 cggcggcgca atacttggtc aaacaggaat ttggagtagc cgtacacatt aagcggtttt
    30301 tcgagttcgc gctcttcgcg gaagatttcg cctttgccgt aaaccgccgc actggaggca
    30361 taaaggaagg ggatgcgttc gtcctgacac cagtccaaca aatccagcgt gtactggtag
    30421 ttgttttcca tcatatacaa accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg
    30481 aaaacggctt cgatgttttg ataaggtaaa atgtgttccc tcacttggcg gataaattcg
    30541 tgtttgtcga ggtagtgggc gatttcgcac tcggcaaggt ttttgaattt ttcgcctttt
    30601 gtcagattat cgacggcgac gatgtcggta atgccgcgtt ggttgagggc tttgacgatg
    30661 ttgctgccga taaagccggc cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt
    30721 tttgagggtt tcagacggca ttggatggtc atgccgtctg aaaagattta ttgcccgtcc
    30781 agtgcttcaa ccaactctgc aaacgagcaa accgccgtac cgagtttcgc cacgacaacc
    30841 ccggccgcag tattggcaag gtacatggct tcgggcatgg tgcagcctgc cgccagcccc
    30901 aagcccattc cggcaatgac ggtgtcgcct gcgccggaca cgtcgtaaac ttcttgggcg
    30961 cgggtgggct ggtaaatggg ttcgccttcg ctgaacaggg tcatgccctc ttcgctccgg
    31021 gtcagcaaaa cggcggtcaa gtcgaggtgg cggcgcaggt tttgcgcttt ttcggtcaga
    31081 tcgccttcgt ttttccaact gccaaccact tctttcaatt cggcgcagtt gggcgtaatc
    31141 agagttgcgc cgacatattt ttcgtaatcg tcgcctttgg ggtcgattaa gacggttttg
    31201 ccgacatgtt tcgcccaatc gatcatatcg gagatatgcg acagaccgcc tttgccgtag
    31261 tccgaaaaaa tgattgcgtc gtattcgggc aagatttcgc ggtatttccg cttgatttgt
    31321 tccaacactt cgcggttggg atgttcttca aaatcaaggc ggataagctg ctggttgcgg
    31381 gcgacgacgc gcagtttgac ggtggtggcg atttgtttgt cgcgcatcag ataggaggcg
    31441 acgccgtcct gcaccatcag cgcgtcgagc gcggcggcgg cttcgtcgtc gccggttacg
    31501 gacaacagcc ctgccctgcc gcccaacgaa gcgatgttgc gcgcaacgtt cgccgcgccg
    31561 cccgcccgtt ggtcgattcg tccgattttt gccaccggca cgggtgcttc gggcgaaatt
    31621 cgggacacgt cgccaaacca atagcggtcg agcatcacgt cgccgacaac aagaactttg
    31681 gcttgtgcga aacgggattt gagggtttct tgttggaact tggcgggcat attgcctccg
    31741 ttgtgttttg ttcagacaac cttaacctgt aagaccgcct gaaaatcagt ttgcttcaag
    31801 gtttgccacg cggttcactt cgcgcaatac ggcgaccgga tcggcggctc gggtaacggg
    31861 gcggcccatc accagataag tcgaacccgc cgccaaggct tcggcgggtg tcataatgcg
    31921 gcgttggtcg tcgttgttgc cggcaacgtc caagcggata cccggtgtaa ccaagacaaa
    31981 atcccgtccc aattcgcggc gcagcggcgc ggcttcttgg gcggaacaga ccacgccgtc
    32041 caagcccgaa ctttgtgcca gttttgccaa acggatgact tgttcttcag gggcggtgtt
    32101 caaaccgatt tccgccaaat cgctttgctc catgctggtc aacacggtta cgccgattaa
    32161 gagcggtttc gtgccgtatc cggcaacggc ttcggcggcg gcttccatca tacgtctgcc
    32221 gcccgacgcg tgcatatcga ccatccacac gcccatatcg gcggcgactt tacacgcctg
    32281 cgcgacggtg tggggaatat cgtggtattt caaatcaaga aagagtttga aaccctgatg
    32341 aatcaggctt tccgccaaac tgcgccccgt agcggtaaac agctctttgc cgattttgat
    32401 ttggcacaac gccggatcaa ggttgcggac gaatccgagc gtgtcttttt cgttggcaaa
    32461 atcaagggca acgatgacgg gggtacgttg ttgcggagtt tggaagtcgg tgattaaagg
    32521 attcatggtg ttttcgtttc gggttttcag acgaccttta tcgtcttttt cgcagtatag
    32581 cggattaaca aaaaccggta cgtcgttgcc ccgccccggc tcaaagggaa cgattcccta
    32641 aggtg
//
LOCUS       DAWTCI010000015        30313 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWTCI010000015 DAWTCI010000000
VERSION     DAWTCI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30313
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.015"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(120..1262)
                     /locus_tag="WM481_000713"
     CDS             complement(120..1262)
                     /locus_tag="WM481_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9080082.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQAGSAPAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTVAITAKGK
                     ELLGGKLPENGWDLLFKPEYTRKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            1566..1829
                     /gene="rpsT"
                     /locus_tag="WM481_000714"
     CDS             1566..1829
                     /gene="rpsT"
                     /locus_tag="WM481_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFA9080083.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            1989..3137
                     /locus_tag="WM481_000715"
     CDS             1989..3137
                     /locus_tag="WM481_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFA9080084.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPTASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPFWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAENLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDWDGI"
     gene            3279..3980
                     /locus_tag="WM481_000716"
     CDS             3279..3980
                     /locus_tag="WM481_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFA9080085.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPQ
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPPEKAELPEQQ"
     gene            4038..5846
                     /gene="aspS"
                     /locus_tag="WM481_000717"
     CDS             4038..5846
                     /gene="aspS"
                     /locus_tag="WM481_000717"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFA9080086.1"
                     /translation="MRTNYCGLISEQYLGQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLGITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDAGNLSNGEDSGLQSPIVKFLSENALKEIIERTAAQ
                     NGDIIFFGADKAKVVNEAVGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTDAPNSVDDKQLRELSLRLRQKAVETKEA"
     gene            5996..6280
                     /locus_tag="WM481_000718"
     CDS             5996..6280
                     /locus_tag="WM481_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9080087.1"
                     /translation="MKKLLLAAVVSLNAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVYDVDADDYWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            6447..8339
                     /gene="speA"
                     /locus_tag="WM481_000719"
     CDS             6447..8339
                     /gene="speA"
                     /locus_tag="WM481_000719"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFA9080088.1"
                     /translation="MPILTIREVCNINHWSIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPFLGFFMVGAYQEILGNMHNLFGDTATADVVVREDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            8438..9361
                     /gene="speB"
                     /locus_tag="WM481_000720"
     CDS             8438..9361
                     /gene="speB"
                     /locus_tag="WM481_000720"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFA9080089.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSFGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            9559..11025
                     /locus_tag="WM481_000721"
     CDS             9559..11025
                     /locus_tag="WM481_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFA9080090.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKVMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFVMSVVNFLIFSVIGSIWWKVLGYW"
     gene            complement(11229..12305)
                     /locus_tag="WM481_000722"
     CDS             complement(11229..12305)
                     /locus_tag="WM481_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFA9080091.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWHIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGIRSHGCVRMKS
                     PDALEFAKTIATGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDTASLKKSIGQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEVSETRTPEVPDVHTPEAQAHLNTQSDGTPTAYTEPAADSSPQVESPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            12369..12506
                     /locus_tag="WM481_000723"
     CDS             12369..12506
                     /locus_tag="WM481_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HFA9080092.1"
                     /translation="MATETNIAEISALDYEGRGVAKVGGKTIFIKGALLDCLMLGCFR
                     L"
     gene            12528..13670
                     /gene="bioF"
                     /locus_tag="WM481_000724"
     CDS             12528..13670
                     /gene="bioF"
                     /locus_tag="WM481_000724"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFA9080093.1"
                     /translation="MKVFRQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSETCIVPYILGGNEATLAK
                     AEYLQGQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAACL"
     gene            13680..14327
                     /locus_tag="WM481_000725"
     CDS             13680..14327
                     /locus_tag="WM481_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HFA9080094.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGMPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRSKFERRICGDKASFEDYQQFPARPFDEIHQELTALFAMIGQDRR
                     TDLIRWTNALFGSGDKIFIPANQHRYWTPRCTVQETDGGHYLFSRFTHWSALWNH"
     gene            14315..15097
                     /gene="bioC"
                     /locus_tag="WM481_000726"
     CDS             14315..15097
                     /gene="bioC"
                     /locus_tag="WM481_000726"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFA9080095.1"
                     /translation="MESLTAINKPRIRQAFQKALSDYDRHALIQQKMTINLIAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVRSRLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKTNGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQAWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFGMPSGKVRLTYHPLFFIARHSAAGRQ"
     gene            15339..16776
                     /locus_tag="WM481_000727"
                     /pseudo
     CDS             15339..16776
                     /locus_tag="WM481_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
     gene            16779..17877
                     /locus_tag="WM481_000728"
                     /pseudo
     CDS             16779..17877
                     /locus_tag="WM481_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            17889..18424
                     /locus_tag="WM481_000729"
                     /pseudo
     CDS             17889..18424
                     /locus_tag="WM481_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            18426..19068
                     /locus_tag="WM481_000730"
                     /pseudo
     CDS             18426..19068
                     /locus_tag="WM481_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
     gene            19089..19448
                     /locus_tag="WM481_000731"
     CDS             19089..19448
                     /locus_tag="WM481_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFA9080096.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSVYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            19540..19615
                     /locus_tag="WM481_000732"
     tRNA            19540..19615
                     /locus_tag="WM481_000732"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19573..19575,aa:Asn,seq:gtt)
     gene            complement(20113..21024)
                     /gene="crgA"
                     /locus_tag="WM481_000733"
     CDS             complement(20113..21024)
                     /gene="crgA"
                     /locus_tag="WM481_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFA9080097.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLEMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLNLTEEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSHFRVVASPEYLAKHGTPQSAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSSCGIACLSDFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVKELGKNMNRTNTK"
     gene            21263..21841
                     /locus_tag="WM481_000734"
     CDS             21263..21841
                     /locus_tag="WM481_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9080098.1"
                     /translation="MNILLLDGGKAFGHSHGGLNHTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYMDEVFTGGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFIGLS
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(22049..23434)
                     /gene="pntB"
                     /locus_tag="WM481_000735"
     CDS             complement(22049..23434)
                     /gene="pntB"
                     /locus_tag="WM481_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFA9080099.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKRGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIHKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLAAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     ISSSGSQEIEEYREVKAADVAEMLKGASSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     IEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDGILAELKK"
     gene            complement(23514..23897)
                     /locus_tag="WM481_000736"
     CDS             complement(23514..23897)
                     /locus_tag="WM481_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFA9080100.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRGFLAHTQ
                     GAAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(23901..25439)
                     /locus_tag="WM481_000737"
     CDS             complement(23901..25439)
                     /locus_tag="WM481_000737"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFA9080101.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFLQ
                     ESGGSGDGCAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNL
                     TKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSARPQQTPSEKAAPAAK
                     PEPKPVPLWKKLAPAAIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYHVVWNVSHSL
                     HTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLNM
                     FKKG"
     gene            25728..26561
                     /gene="serB"
                     /locus_tag="WM481_000738"
     CDS             25728..26561
                     /gene="serB"
                     /locus_tag="WM481_000738"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFA9080102.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLDSRQIDHAVLPNMAFGELGLIVSDMDSTLITIECIDEIAAGVGLKGKVAE
                     ITERAMRGELDFEQSLRSRVALLAGLDEQILADIYENVLKLSPGAEFLLDECKRHNVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANILEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPILKEAGIGVVYRAKPKARAAADACINFGGLERVRGLF
                     G"
     gene            26625..27848
                     /locus_tag="WM481_000739"
     CDS             26625..27848
                     /locus_tag="WM481_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9080103.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIRHTAYGYDADGAY
                     ISFREGVAQAFGMRRVAMLTLCGAIAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEGEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAAFFRRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQYSLELTAVKWAVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            28009..29589
                     /gene="purH"
                     /locus_tag="WM481_000740"
     CDS             28009..29589
                     /gene="purH"
                     /locus_tag="WM481_000740"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFA9080104.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQNLHKLGVEILSTGGTAKLLAGA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPGCTLEDAIENIDIGGPAMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     AGNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYNQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLKAGANRFELKRVGGGLLVQTPDINR
                     INRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMAVTGIRHFRH"
BASE COUNT         7419 a         7398 c         7921 g         7575 t
ORIGIN      
        1 ctataaatgt ccgcaatctg aaatcccggc cgtgggggcg acggtataaa tatttctatt
       61 tccatccaat ataaaatgcc gtccgaacat cgttcggacg gcatttttaa tgcttcgaat
      121 cagttggtgc cgactttgat tttctgccac aggctgaccg acagtttttt cgcatccgtg
      181 ctcatttgcg gcatgacgaa accgtctttc atatcctgct cgttcgggaa gatggaacgg
      241 gtgttcacca actcggcagg cattttttcg cgcgccggtt tgctggcggg ggcgaaggtt
      301 acggcgatgc cgtttttcgc cgcgatttcg gggtcgagcg tgtagttgat gtatttgtgg
      361 gcgttggcga cgtttttcgc atcggcggga atcagccaag actcaatcca gaagcccata
      421 cctttcggtg tcagcacttc gatgccgacg ttgtttttca cttcttcgga acgcgctttc
      481 gccaagttca aatcgccgcc gttgcctgcc gccaggcaga tgtcgccgcg cgccagctcg
      541 tcgatgatgg acgggctgaa acgtttgacg tccggacgga tagacttcaa cacttccgcc
      601 gccgccttca aatcttcagg attcgaacct ttggggtctt tgcccaagta gttcagcaaa
      661 atcgggaaca tttcgctcgg ggtgtcccac aaggcgatgc cgcaggattt cagcttgcgg
      721 gtgtattcgg gtttgaacag caaatcccag ccgttttcgg gcagcttgcc gcccaaaagc
      781 tctttgccct tcgccgtaat cgcaaccgtg ttcacgccgg agaaataggg gacggcatac
      841 tggttgcccg ggtcggcggt ttccagcatt ttcaagagtt cgggatcgat gtttttatag
      901 ttgggaatca ggtctttgtt gactttttga tacgcgcccg cctcgatttg gcgcggcagg
      961 aaggcgatgc ccggcacaac caaatcgtaa ccggatttgc cggtcagcat tttggcttcc
     1021 agcgtttcgt tgttttcgta caagtcgtaa gtcagcttca gattgttggc ttttttaaag
     1081 tcttcgaccg tactctcgtc aacatagttc gaccagttgt agatgttcag agtatcggtg
     1141 gcagcggctt cggcattggc agcaggtgcg ctgcctgctt gaggctgcac ggcgtttttt
     1201 tcgctgccgc cgcaggctgc cagagacagc gcggccaaaa cggctaatac ggattttttc
     1261 atacgggcag attcctgatg aaagaggttg gaaaaaaaga aaaccccgcg ccccatagac
     1321 accccggcgc aaggtttggg cattgtaaag taaatttgtg caaactcaaa gcgatattgg
     1381 cctgattttc ctaaaaaatt accctgtttc caaaaaaggg ggggaaacgg ccgcccgatt
     1441 ttgccgtttt tttgcgccgt cagggtgtcc gacgggcgga tagagaaaaa aggcttgcat
     1501 ataatgtaaa ccccctttaa aattgcgcgt ttacagaatt tatttttctt tcaggagatt
     1561 ccaatatggc aaacagcgca caagcacgca aacgtgcccg ccagtccgtc aaacaacgcg
     1621 cccacaacgc tagcctgcgt accgcattcc gcaccgcagt gaaaaaagta ttgaaagcag
     1681 tcgaagcagg cgataaagct gccgcacaag cggtttacca agagtccgtc aaagtcatcg
     1741 accgcatcgc cgacaaaggc gtgtttcata aaaacaaagc ggctcgccac aaaagccgtc
     1801 tgtctgcaaa agtaaaagca ctggcttgat ttttgcaaaa ccgccaaggc ggttgatacg
     1861 cgataagcgg aaaaccctga agcccgacgg tttcggggtt ttctgtattt cgggggtaaa
     1921 gttcgaaatg gcggaaaggg tgcggttttt tatccgaatc cgctataaaa tgccgtttga
     1981 aaaccaatat gccgacaatg ggggcggaga tgaatacacg aaatatgcgc tatattcttt
     2041 tgacaggact gttgccgacg gcatccgctt ttggagagac cgcgctgcaa tgcgccgctt
     2101 tgacggacaa tgttacgcgt ttggcgtgtt acgacaggat ttttgcggca cagcttccgt
     2161 cttcggcagg gcaggaaggg caggagtcga aagccgtact caatctgacg gaaaccgtcc
     2221 gcagcagctt ggataagggc gaggcggtca ttgttgttga aaaaggcggg gatgcgcttc
     2281 ctgccgacag tgcgggcgaa accgccgata tctatacgcc tttgagcctg atgtacgact
     2341 tggacaaaaa cgatttgcgc gggctgttgg gcgtacgcga acacaatccg atgtacctta
     2401 tgccgttttg gtataacaat tcgcccaact atgccccgag ttcgccgacg cgcggtacga
     2461 ctgtacagga aaaattcgga cagcagaaac gtgcggaaac caaattgcag gtttcgttca
     2521 aaagcaaaat tgccgaaaat ttgtttaaaa cccgcgcgga tctgtggttc ggctacaccc
     2581 aaagatccga ttggcagatt tacaaccaag gcaggaaatc cgcgccgttc cgcaatacgg
     2641 attacaaacc tgaaattttc ctgacccagc ctgtgaaggc ggatttgccg ttcggcggca
     2701 ggctgcgtat gctcggtgcg ggttttgtcc accagtccaa cggacagagc cgtcccgaat
     2761 cgcgttcgtg gaacaggatt tatgccatgg caggcatgga atggggcaaa ttgacggtga
     2821 ttccgcgcgt gtgggtgcgt gcgttcgatc agagcggcga taaaaacgac aatcccgata
     2881 ttgccgacta tatggggtat ggcgacgtga agctgcagta ccgcctgaac gacaggcaga
     2941 atgtgtattc cgtattgcgc tacaacccca aaacgggcta cggcgcgatt gaagccgcct
     3001 acacgtttcc gattaagggc aaactcaaag gcgtggtacg cggattccac ggttacggcg
     3061 agagcctgat cgactacaac cacaagcaga acggtatcgg tatcgggttg atgttcaacg
     3121 actgggacgg catctgaata ccgtttcaaa cggcatatca agttgccgtg ccgtctgaag
     3181 ccgccggcgg tttggcgggg cgggataaaa aattcgtttg aatccgggca aacgcgtata
     3241 atgtgcggtt tgtacggact ttgtcggaat caagcaagat gacggaacct gcggccgaag
     3301 gcggcaaagc tgccaaggcg ttaaaaaaat atctgattac aggcattttg gtctggctgc
     3361 cgattgcggt aacggtttgg gtggtttcct atatcgtttc cgcgtccgac cagcttgtca
     3421 acctgctgcc gaagcaatgg cggccgcaat atgttttggg gtttaatatc cccgggctcg
     3481 gcgttattgt tgccattgcc gtattgtttg taaccgggtt atttgccgca aacgtgttgg
     3541 gccggcagat tcttgccgcg tgggacagcc tgttgggtcg gattccggtt gtcaaatcca
     3601 tctattcgag tgtgaaaaaa gtatccgaat cgctgctgtc cgacagcagc cgttcgttta
     3661 aaacgccggt actcgtgccg tttccccaat cgggtatttg gacaatcgca ttcgtgtccg
     3721 gtcaggtgtc gaatgcggtt aaggccgcat tgccgcagga tggcgattat ctttccgtgt
     3781 atgtcccgac cacgcccaac ccgaccggcg gttactatat tatggtaaag aaaagcgatg
     3841 tgcgcgaact cgatatgagc gtggacgaag cgttgaaata tgtgatttcg ctgggtatgg
     3901 tcatccctga cgacctgccc gtcaaaacat tggcaggacc tatgccgcct gaaaaggcgg
     3961 agttgcccga acaacaataa agccgccgtt cagacggcat tttccgtttt cagtttaaat
     4021 caataaaagg tgattttatg cgtaccaact attgcggcct gatcagcgag caatacttag
     4081 gccaaaccgt taccgtcaaa ggctgggtac accgccggcg cgaccacggc ggtgtgattt
     4141 ttatcgacct gcgcgaccgc gaaggcatcg tccaagtcgt gatcgatccc gacacgcccg
     4201 aagcgtttgc cgctgccgat tccgcccgta acgaatacgt tttgggcatt accggccgcg
     4261 tacgcaaccg tcccgaaggc acgaccaacg acaaaatgat ttccggcaaa atcgagattc
     4321 ttgccaaaga aatcgaagtg ttgaacgccg ccgctacgcc gccgttccaa atcgacgacg
     4381 aaaacatcag cgaaaacgtg cgcctgacca accgcgttat cgacctgcgc cgccctgtga
     4441 tgcagcgcaa cctgcgcctg cgttaccaag tcgctatggg cgttcgccgt tatctggacg
     4501 cgcaaggctt catcgacatc gaaacgccga tgctgacccg ctccactccg gaaggcgcgc
     4561 gcgactacct cgtaccgagc cgcgttcatc cgggcgagtt tttcgcgctg ccgcaatcgc
     4621 cgcaattatt caaacaactg ttgatggtgg cgggtttcga ccgttactac caaatcacca
     4681 agtgcttccg tgacgaagac ttgcgcgccg accgccaacc tgaattcact caaatcgact
     4741 tggaaacctc gttcctgaac gaggatgaaa tcatggacat cactgaaggc atggccaaac
     4801 aagtcttcaa agatgctttg aacgtagatt tgggcgactt cccacgcatg ccttattctg
     4861 aagccatgtt ctactacggc tctgacaaac cggacatgcg catcaactta aaattcaccg
     4921 agttgaccga cctgatgaaa acggaagaat tcaaagtctt ccgtggcgca gccgacatga
     4981 aaggcggccg cgtagtcgct ctgcgcgtgc ctaacggcgc caaattcagc cgcaaagaaa
     5041 tcgacgaata cactaaattt gtcggcatct acggcgcgaa aggtctggca tacatcaaag
     5101 taaacgatgc gggcaacctc tccaacggcg aagacagcgg cctgcaatct ccaatcgtaa
     5161 aattcttgtc tgaaaacgcc ctgaaagaaa tcatcgagcg taccgccgcg caaaacggcg
     5221 acatcatctt cttcggtgcg gacaaagcca aagtcgtgaa cgaagccgtc ggcgcattgc
     5281 gtatcaaagt cggcttggaa cacggcaaag acaacggcta cttcacagac gaatggaaac
     5341 ctttgtgggt tgtcgatttc ccgatgttcg aatacgacga agaagccgac cgctacgttg
     5401 ccgtacacca tccgtttacc gctcctaaag aaggtcatga agacctgatg gtttccgatc
     5461 ctgcaaactg cctggcccgt gcctacgata tggtattgaa cggctgggaa atcggcggag
     5521 gctctatccg tatccaccgt gcagacgtac aagagaaagt gtttgccgcg ctgaaaatca
     5581 gccctgaaga gcaacaagag aaattcggct tcctcttgga caacctgaaa ttcggtgcgc
     5641 ctcctcacgg cggccttgca ttcggcctcg accgtctggt aacgctgatg accggtgccg
     5701 aatccatccg cgacgtgatt gccttcccga aaacacaacg cgcccaatgc ctgctgaccg
     5761 atgcgcccaa cagcgtggac gataagcagt tgcgtgaatt gagcctgcgt ctgcgtcaaa
     5821 aagcagttga aacaaaagaa gcataaggaa aacagagccg tttgacggct ctgttttttc
     5881 agacggcatt tacgcttctt gacttccctc taattcaaac ctaattttgc tgtgttttaa
     5941 tggcggtatt gaaaaacaca tcttgttcaa tcaaccgata aaaaaaggac tgaaaatgaa
     6001 aaaactgtta ttggccgccg ttgtttccct aaatgccgca accgcatttg ccggcgactc
     6061 tgccgagcgt cagatttacg gcgatcccca ttttgaacaa aaccgcacaa aagccgtgaa
     6121 aatgttggaa cagcgcggtt atcaggttta cgatgtcgat gccgacgact actggggcaa
     6181 acctgttttg gaagtggaag cctataaaga cggccgcgaa tacgacatcg tgttgtctta
     6241 ccccgacctg aaaatcatca aagagcagct cgatcgctga ctcctttgat ggaaagatga
     6301 accaaaatgc cgtctgaagc gttcagacgg cattttgcct gttcctcatc aggtatgagg
     6361 caggcttttc ttattaaaaa aatgacattt cacgctgatt tgttataatc attccttttc
     6421 aacacgacag acggagcagg tttattatgc ctatccttac catccgtgaa gtgtgcaaca
     6481 ttaatcattg gagcataggt tattatgatg ttgacgattc cggcgaaatc atcgtccgcc
     6541 ccaatccctc gcaacacaat caaactgttt cactgcaaaa actgactgaa gccgtgcaac
     6601 aaaaacatca ggcgcgcctg cctgttttgt tttgttttcc gcaaatcctc gaacaccgcc
     6661 tccgcgacat taaccgcgcc tttcagacgg cacgggaaga gtgcggctat aagggcggtt
     6721 attgtttggt ttaccctatc aaggtcaacc aacaccgccg cgtcatcgag tcgcttatgt
     6781 caagcggaca accgcatggt ttggaagccg gttctaaagc cgaactgatg gcggttttgg
     6841 cacacgccgg cacccggcaa acattaatcg tctgcaacgg ctataaagac cgtgaatata
     6901 tccgtttcgc cttgatgggc gaaaaactgg ggcatcaggt ttatttggtg attgagaagc
     6961 tgtccgaaat acaaatggta ttggaagagg cggaaaaact cggcatcaag ccccgtttgg
     7021 gtgtgcgcgc cagactggct tcccaaggtt cgggaaaatg gcagtcttcg ggtggggaaa
     7081 aatcaaaatt cggcttgtcg gcttcccaag ttttgcaact ggtcgatatt ttgaaacaaa
     7141 aaaacaggct ggactgcctg cagcttttgc atttccattt gggctcgcag cttgggaaca
     7201 tccgtgatgt tgccacaggt gtacacgaat cggctcggtt ttatgttgag ttgcacaaac
     7261 tgggggtaaa tatccgctgt tttgatgtag gtggcgggct tggcgtggat tacgaaggaa
     7321 accgcacaca atcggattgt tccgttaatt acagcctgaa cgaatatgcc gccacagtcg
     7381 tatggggcat cagtcaggct tgtctcgaac acgggctgcc gcatccgaca atcattaccg
     7441 agagcgggcg cggcattacc gcacatcacg ccgttttggt tgctaatgtt ataggcgttg
     7501 aacgttacaa accgcgtcgg ctggatgcgc catcgcccga agcaccgcgt gtgttgcaca
     7561 gtatgtggga aacttggacg gatatttccg cctcgcggga aaaacgttcc ttacgcagct
     7621 ggatacacga agggcagttt gatcttgccg atgtgcataa tcagtataat gtcgggctgt
     7681 tgagtttggc gcaacgtgcg tgggcggagc aactgtattt aaatatctgt catgaagtcg
     7741 gcgaattgtt taatgaaaaa caccggtctc accgaaccat tattgacgaa ttgcaagaac
     7801 gttttgccga taagctgtat gtcaatttct cactcttcca atctttgccc gatgcttggg
     7861 gcatagatca acttttccct gtttgtccta ttaccggttt gaatgaaccg attgcgcgcc
     7921 gcgccgtgtt gttggacatt acctgcgatt cagacggtac gattgaccac tacatcgacg
     7981 gcgacggcat cgccggtacg atgcctatgc ctgattatcc cgaagaagag ccgccgtttt
     8041 taggcttttt tatggtggga gcatatcagg aaatactcgg caatatgcac aatcttttcg
     8101 gcgacactgc cactgctgat gttgttgtaa gggaagacgg acaatttacc gtcatcgatt
     8161 acgatgaagg aaacaccgtt gccgatatgc tcgaatacgt ttatcaagat ccgaaagagc
     8221 tgatgaaacg ctatcgcgaa caaatcgaac attcagacct tcctgcctcg caggctatgt
     8281 ctttcttaaa agaactcgaa gcggggctta atggttatac ctatttggaa gacgaataga
     8341 cgcatcaagg tatcggatat gtcgtctgaa gcccgatttt cttactcaaa caccaatcat
     8401 cacgaccgat tgaaaccaat tacaaggaat cattacgatg caatacagca cactggcagg
     8461 acaaaccgac aactccctcg tttccaataa tttcgggttt ttgcgcctgc cgcttaattt
     8521 tatgccgtat gaaagccatg ccgattgggt tattaccggc gtgccttatg atatggcggt
     8581 ttcagggcgt tccggcgcgc gtttcggtcc tgaagccatc cggcgcgcct ccgtcaacct
     8641 cgcttgggag caccgcaggt ttccgtggac atttgatgtg cgcgaacgcc tgaacattat
     8701 tgattgcggc gacttggttt tttcttttgg cgacagcagg gattttgtcg aaaaaatgga
     8761 agcgcacgcc ggcaaattac tttctttcgg caaacgctgt ttgagtttgg gcggcgacca
     8821 tttcattacc ctcccgttgt tgcgcgccca cgcccgctat ttcggcaaac tcgcactgat
     8881 tcattttgac gcgcacaccg acacctacga caacggcagc gaatacgacc acggcacgat
     8941 gttttatacc gcccccaagg aaggcctcat cgacccgtcc cgttccgtac aaatcggcat
     9001 acgcaccgaa cacagtaaaa aattgccttt tactgtgttg tccgccccca aagtcaatga
     9061 agacagtgtt gaagagaccg tccgtaaaat caaagaaacc gtcggcaata tgcccgttta
     9121 cctgactttc gacatagact gtctcgaccc gtcgttcgcc cccgggaccg gtacgcccgt
     9181 atgcggcggc ttgagcagcg acagggcatt aaaaatccta cgtgggctga cggatctcga
     9241 catcgtcggt atggatgttg tagaagttgc cccctcttac gaccaatccg acattaccgc
     9301 tttggccggc gccacaattg ccttggaaat gctttacctt caaggtgcga aaaaggactg
     9361 aacgtccggc atcccccggg ttttcgccgt gccgttcaaa cggcgtattc agtctaatga
     9421 aaattcaaat actgaaacaa aagttgcccg gagccgcata tcggaaagac ggtgaaatat
     9481 cgtaatataa cttataaaac aattagttaa atattatttt tccgattttt cgggacggtc
     9541 ttttttacgg aggtcaatat gatgaaattg ggtttcaaac cgatacccct cgccattgcc
     9601 gcagtattgt gcgccctggt tttggcactg cccgtacccg acggggtcaa gcctcaggct
     9661 tggacgctgc tggctatgtt tgtcggtgtg attgccgcca ttatcggcaa ggttatgccg
     9721 ttgggcgcgc tgtcgattat tgccgtcggg ttggtcgcag taaccggcgt aaccgccgac
     9781 aaaccgggcg cggcgatgag cgatgcgttg agtgcgttcg ccaatccgtt gatttggctg
     9841 attgccatcg cagttatgat ttcgcgcggt ttgctcaaaa cagggctggg gatgcgtatc
     9901 ggatatttgt ttatcgccgt ttttggaaga aaaacgctgg gcatcggtta cagtctcgct
     9961 ctttccgaac tgctgctggc tcccgttacc ccttccaata ccgcgcgcgg cggcggcatt
    10021 atacatccga ttatgcagtc gattgccggc agttacggct ccaatcccgc aaaaggcaca
    10081 gaaggcaaga tgggtaaata tttggctttg gtcaattatc attccaatcc catttcgtcg
    10141 gctatgttta ttactgcaac tgcccccaac cctttaatcg tcaacttgat tgccgaaaat
    10201 ttaggcagta gtttccgtct ttcttggggg gcgtgggcgt gggcaatggc tgttcccggc
    10261 gttatcgcct ttttcgttat gcctttgatt ttatattttt tgtatccgcc tgaaattaaa
    10321 gaaacgccca atgctgttca atttgccaaa gaccgtctga gcgagatggg taaaatgtcg
    10381 gcagacgaaa tcattatggc ggtcattttc ggtatcttgc tgctgttgtg ggcagatgtt
    10441 cccgccctta ttaccggcaa tcacgctttt agtatcaacg ccaccgccac cgcatttatc
    10501 ggattaagcc tgcttttgct ttccggtgta ttgacttggg acgatgtttt gaaagaaaaa
    10561 agcgcgtggg atacgattat ttggtttggc gcattgatta tgatggccgc atttttaaat
    10621 aaactcggac tgattaaatg gttctccgga gtgttggcgg aaagtgtcgg cggtttgggc
    10681 gttagcggca cggctgcggg cgtaatcctc gtgcttgctt atatgtatgc gcattatatg
    10741 tttgccagta ctactgcaca tattaccgct atgttcggcg catttctcgc tgctgccgtt
    10801 tcactgaatg ccccggcgat gccgaccgcg ctgatgatgg cggccgcatc caacattatg
    10861 atgaccctca ctcattatgc gaccggtact tcacctgtga ttttcggctc gggctacacc
    10921 acaatgggag aatggtggaa ggcgggtttt gtcatgagcg tagtcaattt tctgattttt
    10981 tccgttatcg gcagcatttg gtggaaagtt ctgggatatt ggtaaaggga aaaataaatt
    11041 tccaatctgt gtttatttga ttgggcgact attatcgtga aatatgccgt ctaaagcctt
    11101 cagacggcat atttgtgcgc ttggatgttg cagaaagcgg cagggcggcg gtgtaggaaa
    11161 agccaaacaa aaaccaaacc gcctgtcaac ttctgataaa cataagtatt aaataatcag
    11221 aaggttattc aattacctaa acgcaaattc ccctgccgta tcacatctat tgaaaataat
    11281 acatcaaccg gctcggaagc agcctgatca ggtgattcta cttgcggcga tgaatcggca
    11341 gccggttcgg tataggcagt cggcgtgccg tcggattggg tatttaaatg cgcttgtgcc
    11401 tcgggcgtgt gtacatcagg cacttcgggc gtgcgtgtct cggatacttc ggcagagttg
    11461 gtttcctcag tttgttcaat gacttcagct tggctgtatg aggaagaacc ctgtatccac
    11521 gccagcgatt tgagcggcat cttcatcttg ccgtttttgc cgcaggtcag acagacggcc
    11581 gatccggtgc ggtctttgag tacggcatcg actttctcgg gcgcgatggt tttgccctgt
    11641 gtttttgccc attgcccgat gctttttttc agggaggcgg tgtcaaggtt gttcttaccg
    11701 taagggtcgc ggaaggcggc aagccacagg ttgcgatccg catcttcatt gaggcccgcc
    11761 atttgataga tgacggaggc gggcgagccg gtggcgatgg ttttggcaaa ctcgagcgcg
    11821 tcgggcgatt tcatgcggac gcagccgtga ctccggatgc ccgggacgct ggccggtgca
    11881 ttggttccgt gtatgcccaa accgagtttc gggtctccta agcggacaaa aaccggcccc
    11941 aaagggttgt ccgggccggc ggctatggtt tttacgccgt cgccgcgttc tttctgtatg
    12001 gattttggga tgtgccaaac agggttatag gctttcgccc cgattttatg ttcgcctaga
    12061 ttggtttgtg tcatcgcccg acctactgca acgggataaa ccttggtcag tttgccgtcg
    12121 gtgtagagga acaggcgttg ctgagggatg ttaatgaaga catgttgacc ttgtgcgacg
    12181 ggggagacat cgggaatgac ggtgtttgcg tatgaaaaac cgcttatcaa tagtgcggca
    12241 gtgcggcaga ttgttttatt catatcaaaa tatggtgtgt gtccgatagg ttttcggcaa
    12301 atcatacctg aaaccgtacc aatttgtgcg aaaatatgcg cttcggtaca gtgcggacgg
    12361 attgggtaat ggcaacggaa acaaatatcg cggaaatttc cgccttggat tatgaaggca
    12421 ggggtgtggc aaaggtcggc ggcaaaacga ttttcattaa aggggcatta cttgattgtt
    12481 tgatgctggg ttgcttcagg ctttaactca ggaatattta catcataatg aaagttttta
    12541 gacaacagct tgaacaactc ggcgcgcaaa atcaatatcg ttcgattccg gatttgattc
    12601 atcaagggcg gtatattacg cgggaaaacc gcaaaatgct gaatatgtcg tctaatgatt
    12661 atttggggtt ggcatcagat gaaaacttac gccggtcttt tttgcagcaa tacggcggta
    12721 attttccctc ttttaccagt tcttcatcac gtttattaac gggcaacttt cctatttata
    12781 ccgatttgga agagcttgtc gcacaacgtt tccaacggga aagcgcgtta ttgttcaaca
    12841 gcggctatca cgccaatctc ggtattttgc ctgctttgac gacgacgaaa agtttgattt
    12901 tggcagataa attcgttcac gccagtatga ttgacggcat ccgtttgagc cggtgtgcgt
    12961 ttttccgtta tcgtcataat gattatgaac atttgaaaaa tctgcttgaa aaaaacgtcg
    13021 gaaaatttga ccgcactttt atcgttaccg aatctgtttt cagtatggac ggcgatgtgg
    13081 cggatttgaa acagcttgtc caattaaaaa aacagtttcc caatacttat ctttatgtgg
    13141 atgaagccca cgcaatcggt gtttatgggc aaaacggatt ggggattgcc gaacgggata
    13201 atctgattgc cgagattgat ttattggtcg gcactttcgg taaagcctta gcctcggtgg
    13261 gggcgtatgc cgtctgcaac caagtattga aagaatgttt gattaatcaa atgcgcccat
    13321 tgattttttc aaccgcattg ccgccgttta atgtggcttg gacttatttt atttttgaac
    13381 gattgccgca attctcaaaa gaaagaagtc atcttgagca gttaagcgca tttttacggc
    13441 gggaagtggc gcatcggacg caaataatgc cgagcgaaac ctgtatcgtc ccctatattt
    13501 taggcgggaa tgaagccacc cttgccaaag cggaatatct gcaaggtcag ggttattatt
    13561 gcctgcccat cagaccgccg acagtaccaa aaaacacatc cagaatccgc ctgtctttaa
    13621 ctgccgatat gacaacggat gaagttcggc agtttgcggc gtgcctgtaa ggatatgata
    13681 tgaaaacaaa attttacaat catcaaggcg aacatttaat cctgtatttt gcaggttggg
    13741 gaatgccgcc cgatgctgta aatcatttga ttttgccgga aaatcacgat ttattgattt
    13801 gctatgatta tcaagattta aatttggatt ttgatttttc cgcctatcgg cacatccgtt
    13861 tggtggcgtg gtcaatgggc gtttgggcgg cagagagggc attgcaagga ataagattaa
    13921 aatccgcaac ggcagtgaat ggcacaggtt tgccttgcga tgataatttc ggtatccctt
    13981 gcgccgtttt taaaggcaca ttggagaatc tcacagaaaa tactcgttca aagtttgaac
    14041 gtagaatctg tggcgataaa gcatcttttg aagattacca acaatttccc gcccgtccgt
    14101 ttgacgaaat tcatcaagaa cttaccgcac tttttgcgat gatcgggcaa gataggcgta
    14161 cagatcttat ccgctggaca aacgccttgt tcggatcggg cgataaaatt tttattcctg
    14221 ccaatcagca ccgatattgg acaccgcgtt gcaccgttca agaaacagac ggcgggcatt
    14281 acctgttttc aagatttacc cattggtcgg cactatggaa tcactgactg ccataaataa
    14341 accgcgcatt cggcaggctt tccaaaaagc attaagcgat tatgacaggc acgccttaat
    14401 ccaacaaaaa atgacgatta atctaatcgc gcatttgcaa gattatttgc cggatatgcc
    14461 attggaaaac gtgttggaat tgggctgcgg ctcaggaatg ttgagtgcct tgctgcaaaa
    14521 acagatttca gcgaattatt ggttatttaa tgatttgtgc gatgtacgaa gccgactggc
    14581 tgaaaaattg cctcaatcct ttgattttta ttgcggcgat gcggaaaact ttccttttca
    14641 acgacaattt gacttaatcg caagcgcatc tgccgtgcaa tggtttcacc aacccgacgc
    14701 ttttatcgcc cattgcaaaa caggcttgaa aacaaacgga ttattggcgg ttgcgacctt
    14761 tggcaaagac aatttaaaag aagtccgcca aatcacaaat ataggcttaa attacccgac
    14821 tttatcccaa tggcaggctt ggttagccaa agattttgag cttttatggt gtgaggattt
    14881 taaggtaata ctagactttg atacgccgtt agatgtactc aaacacctta aatatacagg
    14941 cgttaccgcc acgaaccaaa aaaattggac aagaaaaaat ctcaatggat ttattggcga
    15001 ttacttgtcg gcgttcggta tgccgtcggg caaagtgcgc ctgacttatc atccgttatt
    15061 ttttatcgcg cgccactctg cggcgggcag gcaataaggc agcttatggg caaagttatt
    15121 tttatatcgg gtattgatac tgatgtgggt aaaaggtaat atggcgagcc ttatgcagaa
    15181 ggcatattgt caaacgttaa attatggtat gatttaaaat ttacaagtct atttcagtaa
    15241 atcgttaata ataaaagcgg acaatttaat aataaaagcg gataattggc cgttgcaggc
    15301 ggtcagctat ccgctcgtta cggtatcggg ggtttccttt ggaaaactca ttaattatcg
    15361 gtctggcact ggcggtattg ctgatgcttt tggttatgcg tgcgaagcag ggtaagaaaa
    15421 caccgaagcg caaatcccaa ggtgtcggca atacgcaggg gcagccgccc ggaagcaatg
    15481 attcggactg ggtcgatcag acttggtcaa tcggtttcag acggcacgca actcgactgg
    15541 tcttggaacg aaagtgccga gaccgcatcc gccgccgtat ccgcgcaaga agtcgatccg
    15601 cttacggagt atcaggttta taagcaattc ggttatcagg gcaaggctgc cgaatctttg
    15661 gctgcctatc tggacggcat tccggatggt gaagcgaaac ctgagaacct tatccgcgag
    15721 ctgatcgata tcaatctcga agtgggggat gtcgatgttt tggcagacaa tctgcaaaaa
    15781 tacggcagac tgattccttt cgaacttttg gcaaaatata tcgaacaggc attacagcga
    15841 gattcaaacc atttgcgtat ccgcgtcttg gcggaagaag gtttgggata gggcactcag
    15901 gagattgaaa aacgtgcgga aggcggttct gcgacggcag cttccgcatc gcccccgccg
    15961 gatgtcggcg gtaaggctta tggagccgaa gaaatcaagc gcatcccgat tgtgcggggc
    16021 aaaaaagacg tgtccggaat cagccaagag aaaatcggtg cgattgccgg cctggtccgc
    16081 gccgatcaag gtgcgaaaat ccttaaagac aaagtcagct acgaaacggc atcgaaacaa
    16141 tacgaccgtg ccatccaaac ttccgaaaaa cctgcaaacc tgattatcga tgcgttgaaa
    16201 ctcgattacc aacacgcgga catagaccgt tttgccggac atttgtggaa actttaccaa
    16261 acgttgggca actacggcag gcaggttaaa gagcggatgc tgggttaggg gtacagcctg
    16321 ggttaccatg aagttttcga tgatttggaa aagggggcga acgatcggca aatcaaagac
    16381 atcggtatga ggcacgggta tctgccgaaa aatatacaga aattcaaagc gcaacatcgg
    16441 gatttggtgc ttcaagattc ttcgttgatc aacactggtt cgtctccggc agacgatgcg
    16501 gttaaagaag tagagtcgtt gctgatgtat ggtcagattg aagcggcaat ggatgtgttg
    16561 gagcaggcgg tattgaaata tcccgacgaa tcccagcttt atattacgtt gatcgatatt
    16621 tatgaacgta ctgaagattg ggataggttg gggcagtttt taagggtatt gagggaacgt
    16681 gcggacaggc ttcctgaaga ggtcgttatg ctgatgagcc ggctgctgca gcgtatgaat
    16741 caaaatatta aaaaaataaa acggtacgga aaataaaaat ggaagttcaa ctgccgaaaa
    16801 ttaaaacagt acgcgtaatg ttggcgggga tgacggcgca gcaggaatcc gttttcaaaa
    16861 tggcattcaa aatgcacaat accacccgtt atgaaacagt atccccttca gacggcagtg
    16921 ccgtgcccga tttggttttg gcggataccg atgccgaggg cggttttgaa ctttggaaag
    16981 agcttgccgg gcgttataag gctatacccg tcgccgtctg ttcggagaaa gttcccgatt
    17041 ctgaagttcc ctacctgccc aaaccgattc ggtttgaaac attgtttcct atgctccgca
    17101 agttattgca gggcgagaat gtttatggga aatcgtttat tgcacccgca gaccggtcgg
    17161 cgaaaaacaa cgggaatgtg cagcgtacgg ttacgatacg ccagtttaac ccgaataaag
    17221 gattattggg ggcgttgcgg tttgcggaaa agaacaggca ggacatcgct atcttgcatg
    17281 gaaataagcc ggtccttaat tgttttcccc tcgatacaac gggttttgct gacagaaagt
    17341 gtgtaaaaac tcgaagaatt gtgcaaagac gaaaatttgc aggtcagttg caagactgtt
    17401 cccgataact cgcaatggcg cgaaaaggct aaagtaggca ttatgtcctg tatgtggcag
    17461 ttttccattt ggacggcgca ggacaggttg atttatccga tttttcccga tactccgttt
    17521 acgttgaaat cttggccaaa cctgacccgg taggcaaatg tgccggggtc gatacgcttg
    17581 tcggcatttt tgaccaaggc atccgtcaac cttaacgtgt tgtataaagt gatgccttta
    17641 aacctcaatg atattctgaa ttatcttgcg gcaacctata caaccgggtt tttgtcggta
    17701 gatttgaaaa cggtttcaca acaggcatac tccgatatgg cggataaaat aaatatcggg
    17761 gccgattctg cttctgatag tgaaatgatg aaaaaagcgg aaaaaatcac gacaccatcc
    17821 caatcccagt cgcgcggcct tctgcaaaga ctgatgaaaa aactgttggg cagctaagag
    17881 gcggagagat gagagaaaat aaaattattt tcacaggacc tgtcggcgta gggaaaacca
    17941 ctgccattgc ggctatttcg gacgaagcac tcgtccagac cgatgcttcc gcatccgaca
    18001 tgactttgga taggaaaagg aatacgacag tggcgatgga ctacgggcca tcagcttgga
    18061 tgaggatacc aaagtccatt tatatagtac gcccggtcag gaacggttca actttatgtg
    18121 ggaaatctta agccaaggca gtatgggttt ggtattgctt ttagataatg cccgaaccaa
    18181 ctcgttgaaa gatttggaat tctttttaca ttcgtttcga gggctgctgg agaaggcatc
    18241 cgtcgttgtc ggtattacca agatggatat acgctctcag cccggtatcg acgtgtatca
    18301 caaatatctt gcaaaacata atcttaatgt tccggttttt gaaattgatg cccgtaagga
    18361 agatgacgta aaacaattgg ttagcgcaat gttattttct attgatccgg gactggaggt
    18421 ttaatatgga atcaacactt tcactacaag cgaatttata tccctgcctg actcctgccg
    18481 gtgcatttta tgccgtatcc agcgatgccc ccagtgccgg taaaactttg ttgcgcagcc
    18541 tgttgaaagc ggatgcggac gaagtggtca gcagtgagaa gctgctcgcg ggcggacacc
    18601 gccgacatcg ataccgcttt gaacctgttg taccgtttgc aaaaactcga attcctctat
    18661 ggcgatgaaa acggtcattc agacggcatc aatttgtcgg acgagcaatt gccgttgctg
    18721 atggaacaat tgtccggcag cggtaaggca ttattggtcg atcggaacgg tctgtatctt
    18781 gccaacgcca atttccatca tgagtcggcg gaagagttgg ggttgttggc ggcagaagtc
    18841 gcacagatgg aaaagaaata ccggctgctg attaggaaca acctgtatat caacaataac
    18901 gcttggggcg tttgcgatcc ttccggtcag agcgaattga catttttccc attgtatatc
    18961 ggttcaacca aatttatttt ggttatcgcc ggcattcccg atttgagcaa agaggcattt
    19021 gttactttgg taaggatttt ataccgccgt tacagcaacc gcgtgtaaaa cttgggacag
    19081 gagggattat gcagcaatta ttgatttcaa tccttgaaga tttaaataat acatctacgg
    19141 atattatcgc gtctgccgtt atctcaaccg acggattgcc gatggcgaca atgcttcctt
    19201 cacatttgaa ttcggacagg gtaggggcga tttctgccac tttgcttgct ttggggagtc
    19261 gctcggtgca ggaactcgcc tgcggggaat tggaacaagt gatgattaaa ggaaaatcag
    19321 tctatatcct tttaagtcag gcgggtaaag atgccgtgtt ggtgctggtg gcaaaagaaa
    19381 ccggcagact tggtttaatc ctattggatg ccaaacgtgc ggcaaggcat attgcggaag
    19441 ccatataaca tataaagatt gcgggcttgc agataagtgc aatcgattgt caatttatat
    19501 tgacacgttc ggtatttctg ttttattatt cgcgtttact ccccgatagc tcagtcggta
    19561 gagcgacgga ctgttaatcc gcaggtccct ggttcgagcc caggtcgggg agccaaattc
    19621 caaaaccctc taagtatttt cttagagggt tttgttttac cggcggtcag aaacgcattt
    19681 ttgagatgat tgttttgaga tggaataaaa tctttgcaaa attcctttcg tgatggttat
    19741 gaaaaataaa tttctaaaga tttaattggt tatttttata attttgaaat ttatcggttt
    19801 tgtaaaggtc tcgggataag caatttcggt cttcggcgcg tcttttacgc caaacccata
    19861 agaattttga gggtaatctt catcgttttg cctgaattgc cgacgcaaag cgatttatcg
    19921 gcggttgtgt ttgagcgttt gattgcagct tgcccaatct caaatatttc taaataccaa
    19981 ttgaaattca atattttata aaaacactga tttggaacga acagatttat aaaaattccg
    20041 tgtatccttg agtaagggag ttgattgaaa agacttgttt gattgatgat gaagaataag
    20101 ttaattaatg tcttatttgg tattcgttct attcatattt tttcccagtt ccttcactaa
    20161 aaaatccaaa aatacgcgta agcggaggtt gacggcttta tcgctgtaat aaacagcatt
    20221 aaaggggtgt gttttattgg aggtttgttc ggcgaatagg ggaattaact ttccttcagt
    20281 gatgtcgttg tcaaccaaaa aatctgataa gcaagcaata ccgcaacttg aaaggcacaa
    20341 cgagcgtaag atttcaccgc tgctggcggt aaagtgcggt gaaattttat agggatttcc
    20401 ctgcgcatct aaaaccgccc atgtatttag agaaccgggt tctgtgaagc ctaaacattg
    20461 atggttggca agatcttctg cagattgtgg cgtgccgtgt tttgctaaat attcaggact
    20521 ggcgactacg cggaagtggc tgtcaaacag atggcgtgca cgcagcccgg aatcgtccaa
    20581 ttctccggcc cgtaaggcaa tatcgacttt gcgttcaatc agattgatat agccttcgga
    20641 agaaacgagc gaaagtcgga tatgcggata gcgttcgttg aattttgctg ccagcggcgc
    20701 cagcagatgc agcaccatcg gcatcgcgga atccacgcgc aacacgcctt gcggtacttc
    20761 gtgcactgcc agcatttcgg tttccgccgc tgccatttct tgcaggattc tctgcgcgcg
    20821 gcggaaatat tgcgcgcctt cttccgtcag attgagttgc cgcgtggtgc ggttgagcag
    20881 gttcacgccc aacttttcct ccagccgttt gacgatgcgg cttacggcag aatttgccat
    20941 ctccaactgc tccgccgcac ggctgaagct gccgctttcc accacttgaa caaatacggt
    21001 cagttcttct gaattggttt tcatcgtgtt tccttttcgg ttggaacccc accctttagg
    21061 gcggcaggat cagactttat ttgggaaggg cgtaacccct tcccaatcag gacggcacat
    21121 agggagacga tttatgtgtc atcccgtgtg ttgaaacatc tgattattcc atttgacgca
    21181 aaagtgtttt cttatttttg cacttttaaa tcataaagta aaacggtaca atacattcat
    21241 caattcacaa acgaggtaac aaatgaatat tttattatta gacggcggca aagcgttcgg
    21301 acattctcac ggcgggttaa accacacgct tcacaaaaaa gcgaaagaag ttttgaccgc
    21361 gctcggacac aatgttcaag aaaccgtgat tgatgccggc tatgatgttg aggcggaaat
    21421 cgaaaaattc gtttggatgg atgctgtgat ttggcagatg ccgggctggt ggatgcacga
    21481 gccttggaca gtgaaaaaat atatggacga agtctttacc ggcggacacg gcaaactcta
    21541 ccaaagcgac ggcagacacc gcgtcaatcc gactgagggc tacggcacag gcggcttgtt
    21601 gcaaggcaaa aaacatatgc tttcactgac ttggaatgcg ccgattgaag cctttacccg
    21661 cgaaggcgat ttctttgaag gcaaaggcgt tgatgttttg tatatgcact tccacaaagc
    21721 caacgagttc atcggcttga gccgcctgcc gacattctta tgtaacgatg tggttaaaaa
    21781 tccgcaagtg gaaaaatact tggcagatta tcaggcacac ttggaaaaag tgttcggcta
    21841 attaaaaatc catcttcaac acggagatgg attttgtttg tttcgttgat tttgtgtcag
    21901 tttcagatgt gggtgcttat ccggacgggt cgtctgaaaa tgtttgcccc aatgcaaaaa
    21961 aatcactgca aaccttcaca aacggggctg gtagtgattt tttcaaatca aacagattga
    22021 aaacctgcgc cgaattgttc agacggcatt attttttcaa ttccgccaaa atcccgtcaa
    22081 cggttttctt cgcgtcgccg aaacacatca cgctgttttc gttgaagaac agcgggtttt
    22141 gtacgcctgc gtagccggtg ttcatcgagc gtttgaatac gacgacttct tttgccttcc
    22201 atacttctaa aaccggcata cctgcaatcg ggctgttcgg atcggtttgg gcggcagggt
    22261 tgacggtatc gttcgcacca atcaccaaga ccacatcggt ttcggggaag tcgtcgttga
    22321 tttcgtccat ttccaaaaca atgtcgtagg ggactttggc ttcggcgagc agtacattca
    22381 tatgaccggg caggcggccg gcgacggggt ggatgccgaa gcgtacttcg atgccgtttt
    22441 tacgcaaaag ctcggtgatt tcggcaacgg ggtattgtgc ctgcgccact gccatgccgt
    22501 agcccggggt gataatgacg ctgcttgcgc ctttcagcat ttcggcaaca tcggcagctt
    22561 tgacttctcg gtattcctct atctcttggc tgccggaaga cgatatgccg ctgtcgctgc
    22621 cgaaaccgcc ggcaatcacg gaaacaaacg accgattcat ggctttgcac ataatgtagg
    22681 acagaattgc gccgcttgag ccgaccagcg cgccggtaac gatgagcagg tcgttggaga
    22741 gcataaagcc tgccgctgcg gccgcccagc cggagtagga gttgagcata gatacgacca
    22801 cgggcatatc cgcgccgccg atggaggcaa ccaagtgcca gccgaatgcg agggcgatca
    22861 gggtcataat cagaaggatg aagccgctgc cgtcaatgcc gacaaatacg agcagcaaca
    22921 caaacgatac ggcgagcgcc agtgcgttga gcttgtgttt ggcgggcagt tgcagcgggc
    22981 tgctgctgat tttgccgttg agtttgccga atgcggccag tgagccggta aaggttaccg
    23041 cgccgatgaa aatgcccaaa tagacttcga ccagatggat ggtgtgcata tcgtgcgaaa
    23101 cgttgcccgg ctcgatgtag ctgttgaagc cgaccaaaac ggctgccaag ccgacgaagc
    23161 tgtgcagaag tgcaatcagt tcgggcattt cggtcatttc tacttttttg gctttgtgga
    23221 tgccgattgc cgcgccgatg agcatggcga tgataatcca gcccagtccg tgggtattgt
    23281 cggaaaagac cgttacgaag agggcgaccg ccataccggc gataccggaa tagcagcccc
    23341 gtttggcggt ttcctgtttg gacagccccg ccagtgagaa gatgaataaa attgcggcaa
    23401 cgatatacgc cgctgttacg agtcctgaag acatggaaat tctccgattt tcgatgattt
    23461 gttttcaatg ccgtctgaaa aattgacgtt cgtgttttca gacggcatct gtttcaagca
    23521 gccgcgacaa acagtccgac ggtgcaggca aatccgcccg cccacatcag cgagcgcaat
    23581 gctgctttgt ctgcgatgta gcaccagata aaggcgaggc ggaacaggat gaacaatccg
    23641 gcaagcgtgt tgacggttgc ttgtccggca ttgccggttg cgtgtgccgt caaaacggcg
    23701 gcggcaaacg gtgcaaaggc ttcaaaaccg ttttgctgcg cggcgtgggc acgggcggct
    23761 gcgccttgcg tatgtgccag aaaaccgcga ggattgtggt tgtctttaaa ccggaatccg
    23821 cctgcttttt tggcatacgc cgcacaaaaa agcggcaata ggcaggcaat cagaatgcac
    23881 caataggcga aagtcatggc ttaccctttc ttaaacatat tcagcatacg ccgtgtaacc
    23941 gcaaagccgc cgaagatatt gatgccggca atcaggatgg caacaaacga cagcagcgaa
    24001 acgaagccgt tgccctgacc gatttgcagc agcgcgccga cgacgatgat gccggagatg
    24061 gcgttggtta ccgacatcag cggtgtgtgc agcgagtggc tgacgttcca aacgacatgg
    24121 tagccgatga cgcaggcgag gacgaagacg ataaagtggt tcaagaatgc tgcgggtgcg
    24181 accgcgccga cccacagcac caatacggcg gcgatggcgg cgggcgcgag ttttttccac
    24241 aggggaacag gtttcggctc gggcttggcg gcaggcgcgg ctttttcaga cggcgtttgc
    24301 tgcggccggg cggaaacctg aatcggcgga ggcgggaagg tgatttcgcc gtcgcgggta
    24361 acggtcatat tgcggataat cacgtcttcg aagtccagcg tgatttcgcc gtctttgttc
    24421 gggcttaaca gcttggtcag gttcaccaag ttggtggcgt aaagctggga agactgtccg
    24481 gcaaggcggt ttgccatgtc ggtgtagccg atgattttca cgccgttgcc ggttacggac
    24541 aattcgcccg gtcgggtgag ttcgcagttg ccgcccgtcg ccgccaaatc gacgatgacg
    24601 gatccggatt tcatgctttc caccatttct ttggtaatca gcttgggagc gggtttgccc
    24661 ggaatggcgg cggtggtgat gatgatgtcc acttctttcg cctgttcggc aaagagcttc
    24721 atttcggcgg cgataaattc gtcgctcatc actttggcgc agccgtctcc gctgccgccc
    24781 gattcttgca ggaagtcgag tttcaggaac ttaccgccca tcgattcgat ttgttccgcc
    24841 acttccaagc gggtatcgaa cgcgcgcacc actgcgccga gcgaatttgc cgtaccgatt
    24901 gccgccaaac ccgccacacc tgcaccaatc accaaaacct gcgcaggcgg cactttgccg
    24961 gcggcagtga tttgaccggt gaagaaacgg ccgaaggcgt tggcggcttc aatcacggcg
    25021 cggtagccgc tgatgtttgc cattgaagac aaagcgtcca aggcctgagc gcgggaaatg
    25081 cggggaacca tgtccatcgc cagcgcgttg actttcttgg cgcgcaaggc ctcgaccaaa
    25141 gcctcgtttt ggcgcggcca caggaagctg acgatggttt gaccttcttt gagcagcggc
    25201 agctcgcctt cggacggcgc gttgacctta taaattaaag ggcaggccca aaccgccgct
    25261 ttgtcggcaa cggttgcgcc tgctgtttgg taagcggcat cgtccaaact tgccgccaaa
    25321 cctgcaccgc tttcgacaac ggtttcaaag cctagtttgc ccagcagggc aacggtggcg
    25381 ggcgtgcagg cgacgcgggt ttcgccggat aatgactcgc gtgggatacc gattttcatc
    25441 tctgaatcct ttttcgggtt gttcatatgt atcgtgggtt aaatttaaat cggtgcaggg
    25501 cggagcaacg ccgtaccggt ttaaaaccga ttcacttcaa atgttaatat attttagata
    25561 atccccttat aacgaatttt catcaggctg gcaatagttg cgcattttcc cgtgttgtcc
    25621 gacacatact gccgtttgcg tttgaagtcg ttttccgatt gcaggcacgc ccgccgtaac
    25681 attctttgcc gtttcactat ataatccgca ttttttgagc cgccgttatg ccgcaagccc
    25741 tcgtcctcca atttccctcc gccgaagccc tgccttccga cttcccctca cgcctgcccg
    25801 aacctgatta tgctgatgaa aagcgtatgc gttttattgt tgaagaagga ttttctttaa
    25861 gcgaaaaaga cgcggcgtta ctggacagcc gtcaaatcga ccacgccgtt ttgcccaata
    25921 tggcgtttgg cgaactcggg ctgattgtca gcgatatgga ttcgacgctg attaccatcg
    25981 aatgtatcga tgaaattgcg gcaggtgtgg gtttgaaagg caaagtagcg gaaattaccg
    26041 agcgcgccat gcgcggagag ttggatttcg aacagtcttt acgcagccgc gttgcgttgt
    26101 tggcgggatt ggacgaacag attttggcgg acatttatga aaacgttttg aagctctcgc
    26161 ccggtgcgga atttttgttg gacgaatgca aaaggcacaa tgtgaaattc ctgctggtgt
    26221 ccggcgggtt cacgtttttt accgaaaggc tgcaacaacg gctcggcttc gaataccaac
    26281 acgccaatat tttggaaatt gaaaacggca ggttgaccgg ccgtctgaaa ggcagaatca
    26341 tcgacgcgca ggcaaaggcg gatttattgc gcgaataccg cagccgcctc ggattgcagc
    26401 cgcatcaggt gttggcgatg ggcgacggtg cgaacgatat tccgatactc aaagaagcgg
    26461 gcataggcgt ggtttaccgc gccaaaccga aagcgcgggc cgccgccgat gcctgtatca
    26521 acttcggcgg tttggagcgt gtacgcggcc tgttcggata ggcggatagg aaacggatgc
    26581 cgtctgaaag gttttcagac ggcatttgaa cggcaggaac gacagtggga cgcagaaaac
    26641 tttggtttgc cctggcggca gcaggcgtta tcggcggttt ggtcggcatt gtgctgacgg
    26701 aactgatgca cttcatacgg catacggcat acggttatga cgcggacggt gcgtacatct
    26761 cgttccgcga aggtgtggca caggctttcg gtatgcggcg cgttgccatg ctgacgctgt
    26821 gcggcgcgat cgcaggcggc ggctggtggc tgctcaaacg tttcggcaag ccgcaaatcg
    26881 aaatcaaagc cgccttgaag cagccgttgc aggggctgcc gtttctgacg acggttttcc
    26941 atgttctgct gcaaatcata acggtcggac tcggttcgcc gctcggacgc gaagtcgccc
    27001 cgcgtgaaat gactgccgcg tttgcttttg ccggcggcag gcggttgggt ttggacgaag
    27061 gcgaaatgcg cctcttgatt gcctgtgcgt ccggagcggg tttggcggcg gtgtataacg
    27121 tgccgctcgc ctccacgctg tttatcctcg aagcgatgtt gggcgtgtgg acgcagcagg
    27181 cggttgccgc cgcattgctg acttccgtca tcgccaccgc cgtcgcgcgc atcggcttag
    27241 gcgacgtgca gcagtatcat cccgccaacc ttgccgtaaa tacttcattg ctttggtttt
    27301 ccgccgtcat cggcccgata cttggcgcaa ccgccgcctt tttccggcgc accgcccaaa
    27361 aattcccctt catcaagcgc gacaatatca aaatcattcc cttggccgtc tgtatgtttg
    27421 cactcatcgg tgtgattgcc gtttggtttc ccgaaatttt gggcaacggc aaagcaggca
    27481 accaactgac ttttggcggt ttgaccggct ggcaatacag ccttgagttg accgctgtca
    27541 aatgggcggt cgttttgatg gcgctggcgg caggcgcata cggcggactg attacgccgt
    27601 ctatgatgct cggcagcacc atttcttttg cagccgccgc cgcgtggaac agcgtcttcc
    27661 ccgaaatgcc gtctgaaagc gcggcagtcg tcggagcggc ggtgtttctc ggcgtatcgc
    27721 tcaaaatgcc gctgaccgcc attgtgtttg tattggaact gacctacgcg ccgctgtccc
    27781 tgctgctgcc tttgtgcatc ggcatggcgg gggcattggg aacatcggga aaaatgggct
    27841 tcaaatgaat gccgacaaat gccgtctgaa aggtttccag acggcatttc tttgcaaatt
    27901 gtcaacaata tctgcaaaaa cctactgcca aaaaactgaa aatagcggat aataccgccc
    27961 cgaaaaagat cccctttcac catccaaccg tcagaaaagg aagccccaat gtccgtcatc
    28021 aaacgcgccc tgatcagcct gtccgacaag gcaggcgcgg ttgaatttgc ccaaaacctg
    28081 cacaagctcg gtgtcgaaat cctctccacc ggcggcacgg cgaaactgct tgccggtgcg
    28141 ggcgttcccg tgattgaagt cgccgactac accggttttc ccgaaatgct cgacggccgc
    28201 gtgaaaaccc tgcatccgaa aatccacggc ggtattttgg gccggcgcga tttggacgaa
    28261 cacgtcgcca agatggaaga gcacggcatc ggcaacatcg accttgtgtg tgtcaatctt
    28321 taccccttcg ccgccaccat cgccaagccc ggctgcacac tggaagacgc aattgaaaac
    28381 atcgacatcg gcggcccggc catggtgcgt tccgccgcga aaaactggaa acacgtcgcc
    28441 atcgttaccg acaccgccga cttccccgcc attgccgccg aactcgaagc cggcaacggc
    28501 gcgttgagcg ataaaacccg cttcaacctc tcacgcaaag cattcagcca caccgcccaa
    28561 tacgacggca tgatttccaa ctacctgacc tccctttcag acgacgtttt gagcggccag
    28621 ccccaaatcg gcgaattccc aagccagttc aaccaaagct ggattaaagt gcaagacatg
    28681 cgctacggcg aaaacccgca tcagcgcgcc gcgttttacc gcgatattga ccccgccgca
    28741 ggcagccttt ccgcctacaa tcaactgcaa ggcaaagaat tgtcttacaa caacatcgcc
    28801 gatgccgatg ccgcttggga agccgtcaaa tcctttgacg cgcccgcctg cgtgatcgtg
    28861 aaacacgcca atccgtgcgg cgtagcggtt gcagccgata ccttgaccgc ctacaaactc
    28921 gcctacgcca ccgacaccac cagcgcgttc ggcggcatca tcgccttcaa ccgcgaagtc
    28981 gacggcgaaa ccgtcaaaca gattaccgac aaccaattta tggaagtcct catggcgccg
    29041 aagtttaccg ccgaagccct cgaaatcgcc gccgccaaga aaaacgtgcg cgtattggaa
    29101 gtgccgctca aagcaggtgc caaccgcttc gaactcaaac gcgtcggcgg cggactgttg
    29161 gtacaaaccc ccgacatcaa ccgcatcaac cgcgccgatt tgaaagtcgt ctccaaacgc
    29221 caaccgaccg agcaggaatg gaacgatttg ctgtttgtct ggaacgtcgc caaatacgtc
    29281 aaatccaacg ccatcgtgtt cggcaaaggc ggtcaaacct acggcatcgg cgcaggccaa
    29341 atgagccgcg tggacagtac ccgcatcgcc gcccgcaaag cgcaagatgc cggtctcgac
    29401 ctcaacggcg catgtgccgc ttccgatgcc ttcttcccat tccgcgacgg cgtggatgtc
    29461 attgccgagc agggcatcaa agccatcatc catccggcag gctcgatgcg cgatcaggaa
    29521 gttttcgacg cggcggacga acacggcatc gccatggcgg taaccggcat ccgccacttc
    29581 cgccattaag gcagatgaat aaggcaatgc cgtctgaagg gttttcagac ggcattttgc
    29641 gccatttacc gtaatcctga aatcccgtca ttcccacgca ggcaggaatc tagatgagaa
    29701 tccatcctgt cagaaatacc cgaatcgtca gaaatacccg tgtcatcatt cccgatacac
    29761 cgtaatcctg aaacccgtca ttcccgcgca gacagaaatc cggatttgtc cgcgcggaaa
    29821 cttatgcgcc gccattcccg cgaaggcggg aatccggacc gttcggtttc ggttatttcc
    29881 gataaattcc tgctgctttt cattcctaga ttcccacttt cgcgggaatg acgaaggagt
    29941 gggaatccgg tttttgagtt ccggccattt ccgacaaatt gctttggcat tggatatttc
    30001 ctattttaat ccactatagt ggattaactt taaaccagta cggcgttgcc tcgccttagc
    30061 tcaaagagaa cgattcccta aggtgctgaa gcaccaagtg aatcggttcc gtactatttg
    30121 tactgtctgc ggcttcgccg ccttgtcctg atttttgtta atccgccata tccgatcgcc
    30181 gcaccggaat tttaaaaaat aaatacattc tttaacaaaa aaaatcatat cccggcaaaa
    30241 caaatctgat aaaatacttg gcgtttatta acgatctttt taataattta atcttagata
    30301 tttggagttg ata
//
LOCUS       DAWTCI010000016        28429 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWTCI010000016 DAWTCI010000000
VERSION     DAWTCI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28429
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.016"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            51..1589
                     /gene="murJ"
                     /locus_tag="WM481_000741"
     CDS             51..1589
                     /gene="murJ"
                     /locus_tag="WM481_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFA9080105.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLIVVTALG
                     ILAAPWVIYVSAPGFTKDADKFQLSISLLRITFPYILLISLSSFVGSILNSYHKFGIP
                     AFTPTFLNISFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAAGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRKHGIYRPGRGWAAFLAKMLL
                     ALAVMCGGLWAAQACLPFEWAHAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1722..2678
                     /locus_tag="WM481_000742"
     CDS             1722..2678
                     /locus_tag="WM481_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFA9080106.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQHPAPAYRREMLPDSTGKT
                     KTAYDFSGGISPDAPLVVLFHGLEGSSRSHYAAELMLAVRNRGWHGAVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASA
                     AVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAV
                     TLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRH"
     gene            2729..3511
                     /gene="trpC"
                     /locus_tag="WM481_000743"
     CDS             2729..3511
                     /gene="trpC"
                     /locus_tag="WM481_000743"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFA9080107.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVLLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQGQLERFEALA
                     HELGMTVLLELHDETELEKCRNLTTPLRGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIGAEVGKLF"
     gene            3574..5877
                     /gene="recQ"
                     /locus_tag="WM481_000744"
     CDS             3574..5877
                     /gene="recQ"
                     /locus_tag="WM481_000744"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFA9080108.1"
                     /translation="MTHRPTARQILHEVFGYPEFRGRQEDVINTLAGGGSLTVLMPTG
                     GGKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIA
                     DKLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSRWGHDFRPEYQQLG
                     MLAERYPNIPRIALTATADAATRADIKHYLHLDDASEFVSSFDRTNIYYQVIEKNNGK
                     KQLLDFIRKEMTGQSGIVYCLSRKKVEDAAQFLRENGLNAIPYHAGLSMDVREENQRR
                     FTHEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLC
                     YGLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPC
                     GHCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQL
                     STFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKILKGETEVMLRPL
                     KRDKPATRTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKM
                     PQNLNGLHDIYGLGEAKTERFGHGILKVCQNAADFSHDAVIRPQTEREQQLRQKLEAW
                     RYEQARAENCALHTVLSDESLADMLAATPETETDLEAVHGLGSVRAAKYGRDILAVCR
                     PFSDGIDETAKHKRCLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAEL
                     AAVYGVGEEKAARYGAAVLAVLERDAV"
     gene            complement(5914..7215)
                     /locus_tag="WM481_000745"
     CDS             complement(5914..7215)
                     /locus_tag="WM481_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFA9080109.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLCGEDTQRYASFWGDG
                     YDVYRQLRWRQIPEKQRKAFKKAAKSKNTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYEYLEEYPFITRLCLENHGQTVLDFSNTRITDLSVDMTGVE
                     SLYLNEGLDSLNLKGEIKENCKVCTAGKGAGLILEVGKSVPKVRGLENLTAVNVMGIA
                     DFDMQNLSETYPKLKTIRLWGKPGNIANFSAVSGFEDLEVFTAVDLFGFGADDIPHPD
                     RLPKLHRLWMSSLPEEAAKAVKKLYKKRKEDGLDLWIEKARKPEWLAQNFDNPFRDWD
                     GAEHIPKSHAKKAAELYRKTRAGVVKLLGNPPENTGEGLAEAVKAYTGGFNKMDKKHF
                     IDTVEREDIAEALETILDLIPDGSCADKEKLFEIFDKNRNF"
     gene            7409..7516
                     /locus_tag="WM481_000746"
                     /pseudo
     CDS             7409..7516
                     /locus_tag="WM481_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7597..8385)
                     /locus_tag="WM481_000747"
     CDS             complement(7597..8385)
                     /locus_tag="WM481_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFA9080110.1"
                     /translation="MNPQDKCWQVHRHLAEHTDQRLTLVRNAPGHILLAGADADISRS
                     LLAKRYPPAVFEEYDSRADFLAAAAAARKGGFWQKLTGRGVVQHCQSPTVPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTLAELKSRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAVAWDDENAA
                     RSCVGAIFEREGGLGITLETVYGHAVKKLVLPQGENVVRFFPKR"
     gene            complement(8382..9263)
                     /gene="bioH"
                     /locus_tag="WM481_000748"
     CDS             complement(8382..9263)
                     /gene="bioH"
                     /locus_tag="WM481_000748"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFA9080111.1"
                     /translation="MRKLAKSVLQPAQMPSETPDEPWHNTQRLIVGASAMRRQQERKF
                     MPDAAKKVYLIHGWGANRHAFDDLMPRLPATWPVSAVDLPGHGDAPFAQPFDIEAAAD
                     GIAAQIDTSADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTAAEDYPEGLAAP
                     ALGKMVGAFRTDYAKHIKQFLQLQLLHTPDAAEIIGRILPDLARCGTPQALQEALDAA
                     ERADVRHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLVVMEKAAHAPFLSH
                     AEVFAALYRDFVEGGLT"
     gene            9247..9855
                     /locus_tag="WM481_000749"
     CDS             9247..9855
                     /locus_tag="WM481_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9080112.1"
                     /translation="MASFRTDAANSCPLCFRHVQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRALKHLADLGMAQPLADLTMQNPPDRLSDECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            9920..10384
                     /gene="trmL"
                     /locus_tag="WM481_000750"
     CDS             9920..10384
                     /gene="trmL"
                     /locus_tag="WM481_000750"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFA9080113.1"
                     /translation="MLTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKSLAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMRPGSRSMNLSNTVSVILFEAWRQHGYAGGV"
     gene            10512..11198
                     /locus_tag="WM481_000751"
     CDS             10512..11198
                     /locus_tag="WM481_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFA9080114.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AEFTQTGNASWYGGRFHGRKTSGGDRYDMNAFTAAHKTLPIPSHVRVTNTKNGKSVIV
                     RVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAENKDIFIDLK
                     SFGTEHEAQAYLNQAAQNFAASSSSPNLSVEKRRYEYVVKMGPFASQERAAEAEAQAR
                     GMVRAVLTSG"
     gene            11259..11585
                     /locus_tag="WM481_000752"
     CDS             11259..11585
                     /locus_tag="WM481_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFA9080115.1"
                     /translation="MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQ
                     DKIISRAQAEKAAWARVGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            11667..12251
                     /gene="ruvA"
                     /locus_tag="WM481_000753"
     CDS             11667..12251
                     /gene="ruvA"
                     /locus_tag="WM481_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFA9080116.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAASPAADETEDIVSTLLA
                     LGYNEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            12251..12388
                     /locus_tag="WM481_000754"
     CDS             12251..12388
                     /locus_tag="WM481_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080117.1"
                     /translation="MPSEGGAAFALTKPCVSAPRCLPDGFSRKMALLSLLSDGIVFDA
                     V"
     gene            12414..14270
                     /locus_tag="WM481_000755"
     CDS             12414..14270
                     /locus_tag="WM481_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9080118.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLCRFVWSSMDGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMAFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIIGFASVMRLLIPRLGQTAARQAN
                     ARSLMTGRITDAYSNIATVKLFFHGAREAVYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSAAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGI
                     VNDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFCYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRSGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMEGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            14275..15198
                     /gene="rsgA"
                     /locus_tag="WM481_000756"
     CDS             14275..15198
                     /gene="rsgA"
                     /locus_tag="WM481_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFA9080119.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITAGYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWLEKLKFYETLGYPVI
                     ETRVLENADSLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPHYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELLG"
     gene            15224..15331
                     /locus_tag="WM481_000757"
                     /pseudo
     CDS             15224..15331
                     /locus_tag="WM481_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
     gene            15311..15535
                     /locus_tag="WM481_000758"
     CDS             15311..15535
                     /locus_tag="WM481_000758"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFA9080120.1"
                     /translation="MKKNTPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGTKELNLESDE"
     gene            15519..16415
                     /locus_tag="WM481_000759"
     CDS             15519..16415
                     /locus_tag="WM481_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFA9080121.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSGNEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLERMHSLKTGALIRAAVLLGATACPDLSDAELAVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(16532..16831)
                     /locus_tag="WM481_000760"
     CDS             complement(16532..16831)
                     /locus_tag="WM481_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFA9080122.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTASLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVRRYFWHTRNRE"
     gene            complement(16943..18388)
                     /gene="nuoN"
                     /locus_tag="WM481_000761"
     CDS             complement(16943..18388)
                     /gene="nuoN"
                     /locus_tag="WM481_000761"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFA9080123.1"
                     /translation="MNWSDLNLMPALPEVALLSLLVLLLPADLWASDDKCRWTHYGAL
                     ATVAVTAAVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFVLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWMPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHTGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLVGAFYYLRVVKVMYFDESGRARPAAGGNNAAKS
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(18398..19894)
                     /locus_tag="WM481_000762"
     CDS             complement(18398..19894)
                     /locus_tag="WM481_000762"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFA9080124.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLTGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVVMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(20008..21171)
                     /locus_tag="WM481_000763"
     CDS             complement(20008..21171)
                     /locus_tag="WM481_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFA9080125.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVRQSVMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKNLIKQ
                     CAAGKIDNNDFYGEFNKLWSWKSENKGEGVGNRFQDKTWKRTGKSSDFRKLCIAIQSV
                     FDAPLTERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEENK
                     FKSAKGASEGSKYIDLSMKLRALLAMQSEIKDLAELDVLVQAEYRNRTDIDWE"
     gene            complement(21355..23379)
                     /gene="nuoL"
                     /locus_tag="WM481_000764"
     CDS             complement(21355..23379)
                     /gene="nuoL"
                     /locus_tag="WM481_000764"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFA9080126.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPGATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAVKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLATAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGNFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(23382..23687)
                     /gene="nuoK"
                     /locus_tag="WM481_000765"
     CDS             complement(23382..23687)
                     /gene="nuoK"
                     /locus_tag="WM481_000765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFA9080127.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(23684..24355)
                     /locus_tag="WM481_000766"
     CDS             complement(23684..24355)
                     /locus_tag="WM481_000766"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFA9080128.1"
                     /translation="MTFSVILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKTE
                     GEGKA"
     gene            complement(24367..24846)
                     /gene="nuoI"
                     /locus_tag="WM481_000767"
     CDS             complement(24367..24846)
                     /gene="nuoI"
                     /locus_tag="WM481_000767"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFA9080129.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(24927..26003)
                     /gene="nuoH"
                     /locus_tag="WM481_000768"
     CDS             complement(24927..26003)
                     /gene="nuoH"
                     /locus_tag="WM481_000768"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFA9080130.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPQGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGIVGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            complement(26006..28267)
                     /locus_tag="WM481_000769"
     CDS             complement(26006..28267)
                     /locus_tag="WM481_000769"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HFA9080131.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKFAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSADAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANTPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLPSKLDNRSTWAGEGVQTASDRLVRVGGVGIYHTDSIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMGTIELAGA"
BASE COUNT         6774 a         8296 c         7344 g         6015 t
ORIGIN      
        1 tctgatcctg ccgccctaaa gggcggggtt tcagccgaaa aggaaacacg atgaatatgc
       61 ttggagcttt ggcaaaagtc ggcagcctga cgatggtgtc gcgcgttttg ggatttgtgc
      121 gcgatacggt cattgcgcgg gcattcggcg cgggtatggc gacggatgcg ttttttgtcg
      181 cgttcaaact gcccaacctg cttcgccgcg tgtttgcgga gggggcgttt gcccaagcgt
      241 ttgtgccgat tttggcggaa tataaggaaa cgcgttctaa agaggcgacg gaggctttta
      301 tccgccacgt tgcgggaatg ctgtcgtttg tgctgatcgt cgttaccgcg ctgggcatac
      361 ttgccgcgcc ttgggtgatt tatgtttccg cgcccggctt taccaaagac gcggacaagt
      421 tccaactttc catcagcctg ctgcggatta cgtttcctta tatattattg atttctttgt
      481 cttcttttgt cggatcgata ctcaattcct accataagtt cggcattccc gcgtttacgc
      541 ccacgttttt aaacatctct tttatcgtat tcgcactgtt tttcgtgccg tatttcgatc
      601 cgcccgttac cgcgctggcg tgggcggttt ttgtcggcgg tattttgcag ctcggtttcc
      661 aactgccgtg gctggcgaaa ctgggctttt tgaaactgcc caaactgaat ttcaaagatg
      721 cggcggtcaa ccgcgtcatg aaacagatgg cgcctgcgat tttgggcgtg agcgtggcgc
      781 aaatttcttt ggttatcaac acgattttcg cgtcttatct gcaatcgggc agcgtttcat
      841 ggatgtatta cgccgaccgc atgatggagc tgcccggcgg cgtgctgggg gctgcactcg
      901 gtacaatttt gctgccgact ttgtccaaac actcggcaaa ccaagatacg gaacagtttt
      961 ccgccctgct cgactggggt ttgcgcctgt gcatgctgct gacgctgccg gcggcggccg
     1021 gactggcggt attgtcgttc ccgctggtgg cgacgctgtt tatgtaccga gaattcacgc
     1081 tgtttgacgc acaaatgacg caacacgcgc tgattgccta ttctttcggt ttaatcggtt
     1141 taattatgat taaagtgttg gcatccggct tttatgcgcg gcaaaacatc aaaacgcccg
     1201 tcaaaatcgc catcttcacg ctcatctgca cgcagttgat gaacctcgcc tttatcggtc
     1261 cgttgaaaca cgccgggctt tcgctcgcca tcggcctggg cgcgtgcatc aacgccggat
     1321 tgttgttctt cctgttgcgc aaacacggta tttaccggcc cggcaggggt tgggcggcgt
     1381 tcttggcgaa aatgctgctc gcgctcgccg tgatgtgcgg cggactgtgg gcggcgcagg
     1441 cttgcctgcc gttcgaatgg gcgcacgccg gcggaatgcg gaaagcgggg cagctctgca
     1501 tcctgattgc cgtcggcggc ggactgtatt tcgcatctct ggcggctttg ggcttccgtc
     1561 cgcgccattt caaacgcgtg gaaagctgac cgatgccgat atcctttttc ggaaaacgct
     1621 ttttccgcgc ctatatccgg gcgcgcatcg cccgcgtccg caccggccgg acgggctgcc
     1681 gcatcaccaa tgccgtctga aaacagaaaa accgatacct tatgattttg acaccgccgg
     1741 acacgccctt tttcctccgc aacggcaatg ccgacacgat tgccgccaaa ttcctgcaac
     1801 accccgcacc cgcataccgc cgcgagatgc ttcccgacag cacgggtaaa accaaaaccg
     1861 cctacgactt ttcaggcggc atttcgcccg atgcgccgct ggtcgtgctg tttcacggtt
     1921 tggaaggaag cagccgcagc cattacgcgg ccgaactgat gctcgcggta cgcaatcggg
     1981 gttggcacgg cgcagtcgtc catttccgca gctgcggcgg cgtagcgaac accgccccgg
     2041 tgttctacca cttgggtgat accgccgaaa tcgcctttgc tttggacacg ctcaccgcgc
     2101 gttaccgtga aatatacgcc gtcggcgtat cgctgggcgg caacgcgccg gcaaaatatt
     2161 tgggcgaaca gggcaaaaag gcattgccgc acgcctcggc cgccgtatcc gcccccgttg
     2221 atgcagaggc ggcaggcagc cgcttcgaca gcggcatcac gcggctgctc tacacgcgct
     2281 acttcctccg cacactgata cccaaagcac gttcgctcca aggttttcag acggcatttg
     2341 ccgcagggtg caaaacactg ggcgagtttg acgaccgttt caccgcaccg ctgcacggct
     2401 ttgccgaccg gcacgactac taccgccaaa cttcctgcaa accgctgctc aaacacgttg
     2461 ccaaaccgct gctcctgctc aatgccgcca acgacccctt cctgccgccc gaagccctgc
     2521 cccgtgcgga cgaagcgtcc gaagccgtta ccctgttcca acctgcacac ggcgggcacg
     2581 ccggctttgt cagcagcacc ggcggcaggc tgcacctgca atggctgccg cagaccgtcc
     2641 tgtcctattt tgacagcttc cgcacaaaca ggcattaacg gtttgatgct aatattcccc
     2701 ctttccccgg acaaatacgg aacacgacat gaccgacatc ctcaataaaa tccttgccac
     2761 caaggcacag gaagttgccg cccaaaaagc cgccgtcaac gccgaacaca tccgcgcact
     2821 tgccgcagaa gccgcgcccg tccgcagctt catcgattcg atacgcggca aacaccgcct
     2881 aaacctgccc gccgtcattg ccgaaatcaa aaaggcaagc ccgagcaaag ggttaatccg
     2941 ccccgacttc cgccctgccg agattgcccg cgcctatgaa aacgccgggg cggcgtgttt
     3001 gtcggtactg accgacgaac cctatttcca aggttcgccc gaatacctca aacaggcgcg
     3061 cgaagccgta ttgctgcccg tgctgcgcaa agacttcatc atcgacgaat accaggttta
     3121 tcaggcgcgc gcatgggggg cggatgccgt cctgctgatt gccgccgcac tggaacaggg
     3181 acaattggaa cgctttgagg cgctggcgca cgaattgggc atgaccgtcc tgctcgagct
     3241 gcacgacgaa accgaattgg aaaaatgccg caacctgacc acgccgttgc ggggcgtaaa
     3301 caaccgcaac ctgcggactt ttgaagtctc cctcgaccaa accctgtcgc tgctgcccgc
     3361 gctggaaggc aaaaccgtcg ttaccgaaag cggcattaca ggcaaggcgg atgtggaatt
     3421 tatgcgggcg cgcggcgtgc ataccttcct gatcggcgaa acgtttatgc gtgccgacga
     3481 tattggggcg gaagtgggca aactcttcta aaatcccgat ttcagacggc atattgccgc
     3541 cgaccgacca acacccttac cggacacgga acaatgacgc accgacccac cgccagacag
     3601 attttgcacg aagtattcgg ctatcccgaa tttcgcggca ggcaggagga tgtcatcaat
     3661 actttggcag gcggcggcag tttgacggtg ctgatgccga cgggcggggg caagtctttg
     3721 tgttaccaga ttccggcgct gatgcgcgaa ggcgtggcgg ttgtcgtatc gccgctgatt
     3781 gcgctgatga acgaccaagt ggccagcctg catgtggccg ggattgaagc ggcggcagtc
     3841 aacagcggca catcggcaga tgaggcgcgc gagattgccg acaagcttgc ccaaggccgt
     3901 ctgaagctgc tttatgtcgc gccggaacgc ttggttaccg accgcttttt acgttttctc
     3961 gaccaacaaa ccgtcagcct gttcgccatt gacgaagcgc actgcgtcag ccggtgggga
     4021 cacgatttcc gccccgaata tcaacagctc ggtatgcttg ccgaacgcta tccgaacatc
     4081 ccgcgcatcg ccctgaccgc caccgccgat gccgccacgc gcgccgacat caagcattat
     4141 ctgcacttgg acgacgcgtc cgaatttgtc tccagctttg accgcacgaa tatttattat
     4201 caggttatcg aaaaaaacaa cggcaaaaaa caattgctgg atttcatccg caaagaaatg
     4261 acggggcaaa gcggcattgt gtattgccta agccgcaaaa aggttgaaga tgcggcgcag
     4321 tttttgcgtg aaaacggatt aaacgcgatt ccgtatcatg ccggtttgag catggacgtg
     4381 cgcgaggaaa accaacgccg ctttacgcat gaagacaata ttatcgtggt ggcgaccgtg
     4441 gcgttcggca tgggcataga caaacccgac gtgcgctttg tcgcccatct cgatatgccc
     4501 cagagtgtcg aacatttcta tcaggaatca gggcgcgccg gccgggacgg gctgcctgcc
     4561 gtgagttggc tgtgttacgg cttgaacgat tgggtgttgc tgcgcgaacg gattgccgaa
     4621 ggcaacagcg acgaggtgca aaagcaaatc gaaatgcaaa aactcgatgc catgctttcc
     4681 gtctgcgaaa ccgccgcctg ccgccgcgta ctgctgctca aacatttcgg cgaagcatcc
     4741 gaaccctgcg gacattgtga caactgcctg catccgcccg tacggtttga cggcacggtg
     4801 ttggtgcaaa aattactcag ctgcgtgtac cgcgccggac aacgctttgc cgccggttac
     4861 atcaccaacc ttttgcgcgg caaaagcgac gattggatac gcggcaaccg gcacgagcaa
     4921 ctgtccacat tcggcatcgg cgcggaattg tccgacaaag aatggcgcag cgtcatccgc
     4981 cagtgcatca gcctcggcta cctcaccgtc aacattgccc gatatcaggc attgcaactg
     5041 accgaagccg ccaaaaaaat cctcaaaggc gaaaccgaag tgatgctgcg cccgctcaag
     5101 cgcgacaagc ccgccacccg caccctcaaa gacaactggc tgcgtaccga acgcgaagaa
     5161 cgcctgtggc aggcattgcg cgtttggcgc atgaaacagg ctgaagccga aggcatcccc
     5221 gcctatatga ttttcggcga caaaaccctg cgcgaccttg tcgaaaaaat gccgcaaaac
     5281 ctcaacgggc tgcacgacat ctacggcttg ggcgaagcca aaaccgaacg tttcggacac
     5341 ggcatactca aagtctgcca aaacgctgcc gactttagcc acgatgccgt catccgtccg
     5401 caaaccgaac gcgaacaaca actgcgtcaa aaactcgaag cctggcggta tgaacaggca
     5461 agggcggaaa actgcgccct gcataccgtc ctctccgacg aaagccttgc cgatatgctt
     5521 gccgccacgc ccgaaaccga aaccgacctc gaagccgtac acggcttggg cagcgtacgc
     5581 gccgccaaat acggacggga catcctcgcc gtctgccgtc cgttttcaga cggcatcgat
     5641 gaaaccgcca aacacaaacg ctgcctgatg cgcgccctga ttcaatggtg caacgaaacc
     5701 gcaaaacacg aacagtccga accctaccgc attctcagca aagccgccct gcgcgccatt
     5761 gccgccaaac agccggaagg tttggcggag cttgccgccg tatacggcgt aggcgaagaa
     5821 aaagccgcac gttacggtgc ggcggtgttg gcggtgttgg aacgggatgc cgtctgaagc
     5881 ccgttaccaa gtttcagacg gcattgcctc tgtttaaaaa ttcctgtttt tatcaaatat
     5941 ttcaaacagt ttttccttgt cggcacaaga cccatccggt atcaggtcca atatcgtttc
     6001 caacgcctcg gcaatatctt cccgctctac cgtgtcgata aagtgttttt tgtccatttt
     6061 gttgaaacct ccggtatagg ctttgaccgc ctctgccagc ccctcccctg tgttttcagg
     6121 cggattgccg agcagtttga ccacgccggc acgggttttc cggtacagct ccgccgcttt
     6181 tttggcatgg cttttcggga tatgctccgc gccgtcccaa tcgcggaagg ggttgtcgaa
     6241 attttgcgcc agccattcgg gttttcgggc tttttctatc cacaggtcca gcccgtcttc
     6301 cttccgtttc ttataaagtt tcttcactgc cttcgccgcc tcttcgggca ggctgctcat
     6361 ccacagcctg tgcagcttcg gcagccggtc gggatgaggg atgtcgtccg cgccgaaacc
     6421 gaacaaatct acggcagtga acacttccaa atcctcaaat cccgatacgg cggaaaaatt
     6481 ggcgatattg cccggtttgc cccacagccg tatggttttg agcttgggat acgtttccga
     6541 caggttttgc atatcgaaat cggcaatgcc catgacgttg acggcggtca ggttttccaa
     6601 accccgcact ttgggaacgc ttttgcccac ttccaaaatc agacccgcgc cctttccggc
     6661 ggtacagacc ttgcagtttt ccttgatttc gcctttcagg ttcaggctgt ccaagccttc
     6721 gttcagataa agcgattcca cgccggtcat atcgacagaa aggtcggtta tgcgcgtatt
     6781 gctgaaatcc agcacggtct gaccgtggtt ttccagacac aatcgggtga taaacggata
     6841 ttcctccaga tattcatata gatttttatg ccacttggtc agaaacagcg aagacaggca
     6901 gggaaacgcc ttcagttcca tcgcgtcttc aaaatcatcc caaacatcgc tcaaattctg
     6961 tttggatatg ccgtattccc gtccggcaaa catcacggta tttttgcttt tggcggcttt
     7021 tttgaatgcc tttctctgtt tttcgggtat ctgccgccat ctcaactgac ggtacacgtc
     7081 gtagccgtcg ccccaaaaag aggcataccg ctgcgtgtct tcgccgcaca gcggcgcggt
     7141 attcccaacc cgtacatatc ccgtcggcac gacggcactt gccttataca tgtgcagacc
     7201 gcgttcccaa tacataaagt ctttgtaaag cggtttcaaa tccgccagct ccgccgcccc
     7261 aagcggtttg ttgccccgcc agtccaaagc cagcagggtc gcatgtgttt ttttgccacc
     7321 gtgttcttca actcgggtaa cctggcaggc agtgtactgc ttcagtctga tgttatagac
     7381 gcaatatacg tctccggcat cggcacatag tggattaaat ttaaatcagg acaaggcggc
     7441 gaagccgcgg acagtacaga tagtacggca aggcgagcca acgccgtacc ggtttaaatt
     7501 taattcacta tacatcggca tttcctttat ttctgtcggt tttcacagac aaatgccgtc
     7561 tgaaacttgg aaacggcttc agacgtcatc tgcacatcat ctcttcggaa aaaaacgcac
     7621 cacgttctcc ccctgcggca gcaccagttt tttcacggcg tgtccgtaca ccgtttccag
     7681 cgtaatgccc aaaccgcctt cccgttcaaa tatcgccccg acacacgaac gcgcggcgtt
     7741 ttcgtcgtcc cacgccaccg cccgccaaac gcccagcgtg tccatatccg cccaaaacgt
     7801 ttccgccttt ttgtagtcca acaccagctt cgccgtatcg gtaactggat cgtaaaagcc
     7861 gttttcggcg agcatatcgc ccaagtcgtg catatcggga aacatcacgc tgcggctttc
     7921 aatgccgttt tctttcagac gggatttcag ttccgccaag gtatcgcgcc cgaagcaggt
     7981 gaaaaacagc agcccgtccg tcttcagcgc gcgcgcccag ttgtgcagca cgggaaggat
     8041 ttgttccgcc gccaacagtc cgaggttcga ccacaacata tcggcacacg cttcgggcag
     8101 cggcacggtc ggggattggc agtgctgcac cacgcccctg cccgtcagtt tttgccagaa
     8161 accgcccttg cgcgcggctg cggcagccgc caaaaaatcc gcacgggaat cgtattcttc
     8221 aaataccgcc ggcggatagc gcttcgccaa caggctgcgg ctgatgtccg catccgcacc
     8281 ggcaagcagg atatgcccgg gcgcgttgcg gacgagtgtc aggcgttggt cggtatgttc
     8341 ggcaagatgg cggtgaacct gccagcattt gtcctgcgga ttcatgtcaa acccccttcg
     8401 acaaagtcgc ggtacagcgc ggcaaacact tccgcatggc tcaaaaacgg cgcgtgtgcc
     8461 gccttttcca tcacaaccag cctgcttcct ttcaaatgcc gatgcagata ctcgcccata
     8521 cgcggcggtg taatcgcgtc tttgccgccg aacaccagca gtaccggaac atctatcttg
     8581 tccaacaaat gccgcacatc cgccctttcc gccgcgtcca aagcttcctg taaggcttga
     8641 ggcgtgccgc agcgcgccaa atcgggcagg attctgccga tgatttcggc ggcatcaggc
     8701 gtgtgcagaa gctggagttg tagaaactgt ttgatatgtt tggcataatc cgttcggaac
     8761 gcgccgacca ttttgcccag tgcaggcgcg gcaagccctt cgggatagtc ttcggcagcc
     8821 gtcagccgtg cgaaactcgc cgtcaggcag agcgaacgga ctttgtcggg atggcgcgcc
     8881 gccagataca gcgcgaccaa tccgccgagt gaccagccga gaatgtcggc cgacgtatcg
     8941 atttgagcgg caatgccgtc ggcagctgcc tcaatatcga agggttgcgc gaagggcgcg
     9001 tccccgtgtc cgggcaaatc gacggcggac accggccacg ttgcgggcag gcgcggcatc
     9061 aaatcgtcga acgcgtggcg gttcgccccc caaccgtgta tcaggtaaac ttttttggca
     9121 gcatcaggca tgaattttct ctcttgttgg cggcgcatcg cagacgcgcc cactatcagg
     9181 cgttgcgtat tatgccacgg ttcgtccggc gtttcagacg gcatctgcgc cggctgcaat
     9241 accgatttgg caagtttccg caccgacgcg gcaaacagct gtcccctgtg tttcaggcac
     9301 gttcaaggcg gcgcggtgtg cggcggatgt cagaaaaaac cgcccgcatt cgaccggatg
     9361 tgggcatcgc tgcattacga accgcccgtc agcaatatga tacgcgcgct gaagcacttg
     9421 gctgatttgg gcatggcgca gccgctggca gacctgacga tgcagaatcc gcccgaccgg
     9481 ctttcagacg aatgtttcga tttcgtccta cccgttccgc taagcaggga gcggctgctg
     9541 caacgcgggt tcaaccaaag cgagagcatc gtcgggttgt tggcgcaacg ctacggctgg
     9601 cagatactgc cccgacacac cgtattccga caccaccgcc cgccgcaaag cacgctcaaa
     9661 ggcggcgaac ggcggcgaaa catcaaaaac gcctttgaaa tccgcacacc gataccggaa
     9721 aactgtaata ttttgttaat cgacgatgtc tttaccaccg gcgcgacgct ggacgaattg
     9781 gcaaagacgc tgaaaaaatc gggcgcaaac cgaatctgct gctggacgct ggcacgcacg
     9841 ccaatgaaaa aataactaaa ttttttgaca ccccatccgc cttatggcaa ggttacgcgc
     9901 ctataagtga ttgatattta tgcttaccat cgttttatat cagccggaaa tccccccgaa
     9961 cacgggcaac atcatccgcc tgtgcgccaa taccggcgcg gatttgcacc ttgtcaaacc
    10021 gctcggcttc ccattggatt ccgccaaaat gaaacgcgcc gggctcgact accacgaatt
    10081 cgccagcctg acggtgcacg aaaacttcga cgactgcctc aagtcgctgg caggccgacg
    10141 tattttcgcc ctgaccacca aaggcacggc gcgccccgat gaaaccgcgt ttcaaaaagg
    10201 cgacgtttta ctgttcgggc cggaaacgcg cgggctgcct gccgacatcc tcgacagcct
    10261 gcccgccgcg caaaaaatcc gcctgccgat gcggcccggc agccggagta tgaacctttc
    10321 caacaccgtc tccgtgattc tctttgaagc gtggcggcaa cacggttacg caggcggcgt
    10381 ttgaacgcag gttcatgccg tctgaaaacc gttcggacac attccgaacc gctgccgcag
    10441 cgtgcggcgg ttcggaacgc gtccgtaggg cataatgccc atccgtcttc aactcaagaa
    10501 cggaacacat tttgacttta acccgaaaaa cccttttcct cctcaccgcc gcgttcggca
    10561 cacactccct tcagacggca tccgccgacg cagtggtcaa ggcggaaaaa ctgcacgcct
    10621 ccgccaaccg cagctacaaa gtcgccgaat tcacgcaaac cggcaacgcc tcgtggtacg
    10681 gcggcaggtt tcacgggcgc aaaacttccg gcggagaccg ctacgatatg aacgccttta
    10741 ccgccgccca caaaaccctg cccatcccca gccatgtgcg cgtaaccaac accaaaaacg
    10801 gcaaaagcgt catcgtccgc gtcaacgacc gcggcccctt ccacggcaac cgcatcatcg
    10861 acgtatccaa agccgccgcg caaaaattgg gctttgtcag ccaagggacg gcacacgtca
    10921 aaatcgaaca aatcgtcccg ggccaatccg caccggttgc cgaaaacaaa gacatcttta
    10981 tcgacttgaa atctttcggt acggaacacg aagcacaagc ctatctgaac caagccgccc
    11041 aaaatttcgc cgcttcgtca tcaagcccga acctctcggt tgaaaaacgc cgttacgaat
    11101 acgttgtcaa aatgggcccg tttgcctcgc aggaacgcgc cgccgaagcc gaagcgcagg
    11161 cacgcggtat ggttcgggcg gtactgacct ccggttgacg gttattcgat accttattaa
    11221 tatccccatt tttcaacccc tgatttacaa gggcaaatat gaacatcaaa caccttctct
    11281 tgaccgccgc cgcaaccgca ctgttgggca tttccgcccc cgcactcgcc caccacgacg
    11341 gacacggcga tgacgaccac ggacacgccg cacaccaaca cggcaaacaa gacaaaatca
    11401 tcagccgcgc ccaagccgaa aaagcggctt gggcgcgtgt cggcggcaaa atcaccgaca
    11461 tcgatctcga acacgacgac ggccgtccgc actatgatgt cgaaatcgtc aaaaacggac
    11521 aggaatacaa agtcgttgtc gatgcccgta ccggccgcgt gatttcctcc cgccgcgacg
    11581 actgaatttg atacaatccg tgccgtctgg agcccgaacc ggtttcagac ggcattttgc
    11641 acccgacact tcaggattcg gcacacatga tcagcagact gaccggcaaa ctggttgaaa
    11701 aaaaccctcc gcaaatcgtc atcgatgtca acggtgtcgg ttatgaagcc gacgtatcga
    11761 tgcagacctt ctacaacctg ccgcccgtgg gtgaaagcgt acaactgttt acccagctta
    11821 tcattcggga agacgcacat cttttatttg gttttgccac tgcggaagaa cgcaagacct
    11881 tccgccaact gattaaggtc ggcggcatcg gcgcgaaaac ggctttgggc attttgtcgg
    11941 cgatgacggc agacgagctg gcgcgggcgg ttgcagaaga agatgtcaaa cgcctctcct
    12001 ccgctccggg aatcggcaaa aaaaccgccg aacgtatggt cttggaactg cgcggcaagc
    12061 tggtcgcgca tacggtaacg gacgggctgt ttgccgcctc acccgccgcc gacgaaacgg
    12121 aagacatcgt cagcacgctg cttgcgctgg gttacaacga acgcgaggca aaagcggcgg
    12181 tcaaaggcgt tccgaagggg acggacgtgg gcgaaggcgt gcgccttgcc ctgaaaaacc
    12241 tgctgaaata atgccgtccg aaggcggcgc ggcgtttgcc ctgacgaaac cctgcgtttc
    12301 cgccccgcgc tgtcttcccg acggcttttc ccgtaaaatg gcgttattgt ccctcctttc
    12361 ggacggcatt gtgtttgatg ccgtctgaaa ctgttttacc gaaatcgaaa acaatgttga
    12421 acaaaatatt ttcctggttc gagtcccgaa tcgaccccta ccccgaagcc gcgccgaaaa
    12481 cgcccgaaaa aggcttgtgc cggtttgtct ggagcagtat ggacggggtg cggaaatgga
    12541 tagccgccct agctgcgctg accgccggca tcggcattat ggaagccctg atttttcaat
    12601 ttatgggcaa aatcgtagag tggctcggca aatacgcgcc cgccgaactg tttgccgaaa
    12661 aaggttggga actggcggca atggcggcga tgatggcgtt ttcggtcgtg tgggcgtttg
    12721 ccgcgtccaa cgtgcgcctg caaacccttc agggcgtgtt ccctatgcgc ctgcgctgga
    12781 acttccaccg cctgatgctg aaccagagcc tcggttttta tcaggacgaa tttgccggcc
    12841 gcgtgtccgc caaagtcatg cagaccgcgc tggcgttgcg cgacgcggtg atgacggttg
    12901 ccgatatggt cgtttatgtg tcggtgtatt tcatcacttc gggcgtgatt ctcgcctcgc
    12961 tcgactcatg gctgctgctg ccctttatcg gctggataat cggtttcgct tcggtaatgc
    13021 gcctgctgat tcccagattg gggcaaaccg ccgcacggca ggcgaatgcc cgctcgctga
    13081 tgaccggccg cattaccgat gcctattcca atatcgccac cgtcaaactc tttttccacg
    13141 gcgcgcgcga agccgtctat gccaagcagt cgatggaaga atttatggtt acggtgcgcg
    13201 cccaaatgcg gctggcgact ctgctgcatt cgtgcagctt catcgtcaac acttcgctga
    13261 ccctctccgc cgccgcactg ggcatctggc tttggcacaa cggacaggtc ggcgtgggtg
    13321 cggtcgccac cgccaccgcg atggcgttgc gcgtcaacgg gctgtcgcaa tacattatgt
    13381 gggaatccgc gcggctgttt gaaaacatcg gcatcgtcaa cgacggcatg gcgaccctgt
    13441 ccaaaccgca caccatcctc gacaagcccc aagccctgcc gctgaacgtg ccgcaaggcg
    13501 caatcaagtt cgaacacgtc gatttttgct acgaagccgg caaaccgctg ctcaacggct
    13561 ttaacctgaa tatcaaaccc ggcgaaaaag tcggcttgat cggacgcagc ggcgcgggca
    13621 aatccaccat cgtcaacctg cttttgcgtt tctacgaacc gcaaagcggc acggtttcga
    13681 tcgacgggca ggacataagc ggcgttaccc aagaatcttt acgcgcccaa atcggtttgg
    13741 tcacacaaga tacctcgctg ctgcaccgtt ccgtgcgcga caacattatt tacggccgcc
    13801 ccgacgcgac cgatgccgaa atggtttccg ccgccgaacg cgccgaagcc gccggcttca
    13861 tccccgacct ttccgatgcc aaagggcgga gcggctacga cgcgcacgtc ggcgaacgcg
    13921 gcgtgaaact ctccggcgga caacgccagc gcatcgccat cgcccgcgtg atgctcaaag
    13981 acgcacccat cctgctgctt gacgaagcca ccagcgcgct cgattccgaa gttgaagccg
    14041 ccatccaaga aagcctcgac aaaatgatgg aaggcaaaac cgtcatcgcc atcgcccacc
    14101 gcctctccac cattgccgcg atggacaggc tcgtcgtcct cgacaaaggc cgcatcatcg
    14161 aagaaggcac acacgccgaa ctcctcgaaa aacgcgggct ttacgccaaa ctctgggcgc
    14221 accagagcgg cggcttcctc agcgaacacg tcgagtggca gcacgactga accgatgccg
    14281 tccgaacacc ccttttcaga cggcatttcc acacccaacc ccaaagaaac catgaacgac
    14341 accgcccaaa ttaccgccgg ctacggccgc cgctacatcg tccgcacgcc cgacggcaca
    14401 acctacgaag ccagcacccg caaaaaacgc gtcgatttcg cctgcggcga ccgcgtccgc
    14461 atcagccccg tcaacgccga acaagttgtg attgaagatt ttttaccgcg ccaaagcctg
    14521 ctctaccgcc aagacgcgtg gaaaaccaaa ctcatcgccg ccaacgttac ccaactcctc
    14581 atcgtaaccg ccgccgtccc gagtccgagc gtgcggctgc tacagcgcgc cctgcttgcc
    14641 gccgaagccg ccggcatccg cgccgtcatc gtcctgaaca aagccgacct gcccgaaacc
    14701 gccctctggc tcgaaaaact caaattctac gaaacgctgg gttaccccgt catcgaaacc
    14761 cgcgtgctgg aaaacgccga cagcctgcgc cccgtcctgc aagggcacag caacatcctg
    14821 ctcggacaaa gcggtatggg caaatccacc ctgaccaacg cccttttagg cagccaaacc
    14881 gcccgcaccg gcgacatttc cgccgcactc gactcgggaa aacacaccac cacccacgcc
    14941 cggctttatg atttgaacgg cgaaacccaa ctcatcgact ccccgggttt gcaagaattt
    15001 ggtttacacc acctccaagc cgccgatttg ccgcactatt tccccgattt ccgccacctt
    15061 gtcgggcaat gccgcttcca caactgcacc caccgcgccg aacccggctg cgccttcaaa
    15121 gccgccgccg aaaccggggc ggcaagcccc gaacgcctcg cctttttgca gggcatcacc
    15181 gacgaactgc tcgggtaacg ccttgccgac cggggcggaa aaaatgccgt ctgaagccgg
    15241 attcgggttt cagacggcat ccgttttgca aaaatgctac aatccgcttt ttaccggaac
    15301 acccgaaact atgaagaaaa acaccccgaa atcgtttgaa gaggccttgt cgcgccttga
    15361 atcgctgacg cagtccatgc agggcgaaat gcccttggaa gacgcgcttg ccgcctatca
    15421 ggaaggcaac gagctggtca ggtactgcca aaccaagctg gcccaagtcg aacagaaatt
    15481 acaggttttg gatgcggacg ggacgaagga gttgaacctt gaatccgacg aatgatttga
    15541 aagcgtggca acagagggcg caggcgcaga cagagctgct gctggaacgc tttttgccgt
    15601 ctggaaacga aatcccgcac acgctgcacg aagcgatgcg ctatgcggct ttggacggcg
    15661 gcaagcgtct gcgcccgatg ctggtgctgg cggcttcgga attgggcggg gccgtggcgg
    15721 atgcggtcgg acaggcaatg gcggcaatcg aaatgatcca cgtctattct ttggttcacg
    15781 acgatatgcc ggcgatggac aacgacagcc tgcggcgcgg caaaccgacc tgccacatca
    15841 aatatggcga agccaccgcc cttctgaccg gcgacgcttt acaaacccaa gccttcgacg
    15901 tgttgagccg tccgacagaa ctgcccgccg cacgccagtt ggcaatgttg tcggtgttgg
    15961 caaaagcggg cggcagcgcg ggtatggcgg gcggacaggc aatcgatttg gcaaatgtcg
    16021 gcaaacaaat ggttcaagcc gatttggaac ggatgcacag cctgaaaacg ggggcgttga
    16081 tccgtgcggc ggttttattg ggggcgacgg cgtgtcccga tctgtccgac gcggaacttg
    16141 ccgtattgga cgcttacgcg gcaaaactgg gtttggcgtt ccaagtcatc gacgatgtgt
    16201 tggattgtga agcggacacg gcgactttgg gcaagacggc gggcaaagat gccgacaacg
    16261 acaagccgac ttatgtgaaa ctgatgggct tggaagcggc gcgctcatac gcacacaaac
    16321 tggttgccga agcagtcgcg ctgctcgaac ccttcggcga caaagccctg cgcctgcggc
    16381 agttggcaga atttgcagtc gcacgcaaat attaaaaccg gcgtatatcg ccgactgaaa
    16441 cgtgccggca cagcgatgcc ggcctgcact tgaatgaaac aaatgccgtc tgaaagggtt
    16501 tcagacggca ttcctatgct tgtttgcttg tttattccct gttgcgcgtg tgccaaaaat
    16561 accgccgcac gaaacacggg aaggcgaaaa tgaggtacag gcagacgacg gtggcgtaaa
    16621 actcccaacc ctgattgtgt accgctcccg cacgggattc gaggatgtag gcaagagagg
    16681 cggtcagcgc gaaacctgcc gccagctcga tcaggtggtg tccgaaatgt ttgcgcttga
    16741 gcgcggccac gccgaacagt ctggtcgtga ggaagggggc gttggcaaag atgagtgcca
    16801 agaccaaaag gatgtacatg gatgcggtca tgatgggctt tctgtttggg cattgtcggc
    16861 ggcgatgccg tctgaactgc attgcggaac ggcatatctt aacaaaaacg gcagcctctg
    16921 acacggctgc cgtttcggcg gctcacagcg tgttctccaa cgccttggcg caccagtcga
    16981 taacggtttg cggcataatg ccccacagca ccaacagcag cgcgttgacg cttaagaggg
    17041 attttgcggc attgttgccg ccggcggcgg gacgggcgcg gccggattcg tcaaaataca
    17101 tgactttgac cacgcgcagg tagtagaacg cgccaaccag cgacatcacg acggcaaaca
    17161 ccgacaacca tacatagcct tgtttcaaaa gcgccataat cacgccgaat ttggcgtaaa
    17221 aacccatcag cggcggaatg cccgccatag agaacataac cagcagcatc aaaaaggcaa
    17281 gccatacgcg gtgttggttc aaccctgcca aatcgctgat gttttcgcac tcgttgtccc
    17341 cgtccgacaa caccatcaac acgccgaacc ctgccgccgc catcagcgcg taggtaatgg
    17401 cgtaatagag gcccgccgca aagccgaccg cgcccgccat aaacgccaac aggatgaaac
    17461 ccgtatgcga tacggtggaa taggcgagca tacgtttgat attggtctgc atgatggcgg
    17521 caaggttgcc gaccagcagc gaggcggcgg caagcagggc aaacatcaga gaccagtcat
    17581 gatgcacggt tcccagcccg gtaacgagga tgcggaaagc gaaaacgacg gcggcgattt
    17641 tcggggcagt gccgaccaag gcggtaacag aagtgggcgc gccgtgatac acgtcgggca
    17701 tccacatatg gaacggtacc gcgccgagtt tgaacgcaac ggcgacgacg ataaacacca
    17761 gacccagttt caacagccat tcgttagctt cttcattgaa ggaagaggcg agcacgccgg
    17821 caaattccag cgaaccggtt gcgccgtaaa ccatagaaat accgtagagc agcaggccgg
    17881 atgccagcgc gcccagaaca aaatatttca aggcggcttc ggcggcaaaa ccggaatcgc
    17941 ggcgcagggc aatcagggcg taaagggcga gcgacaagag ttccaaaccg atataggcag
    18001 ttaaaaaatg ccccgcgctc accatcacgc tcatgcccaa gagggcaaac agcgacaggg
    18061 tgtaaaactc gcctttgaaa ataccgcgca cttggttgta gggcttggca tagacaaaca
    18121 ggacaaacgt caaggcatat aaaaccattt ttgccaaacg cgacataccg tccgcaatat
    18181 acatcccgtt gaacgaagac gtgctgccct gttcccacac ggcaagctgc accgccgccg
    18241 taaccgccac cgttgccaac gcgccgtaat gcgtccaacg gcatttgtca tcactggccc
    18301 acaagtccgc cggcaacaat aacaccagta gcgacagcag cgcgacttcg ggcagtgcgg
    18361 gcattaaatt caaatctgac caattcattt acacacctca aatcttgctt tgcgccacat
    18421 gggcaatcaa atcgtttgcc gcctgatgca ccacttcgat aaatgcgttc ggatacaggc
    18481 ccatccccaa aacagcaacc gccaaaaccg ccagaatcgc aaactcgcgg caattgatgt
    18541 ctttcatttc ggcaacgtgc ggattgtgaa tcgcgccgaa aatcacgcgc ttgtacatcc
    18601 acagggtata agacgcgccg taaatcagcg tcatagccgc cagcgcgccc acccagaaat
    18661 tcactttaac cgcgcccata accaccataa actcgcccac gaagccggaa gtcgcaggca
    18721 ggccggcgtt cgccataccg aacaacatca taaacgccgc aaacttgggc atcacattga
    18781 ccacgccgcc gtaatcggca atattgcgcg tgtgcaggcg gtcgtacatc acaccgatgc
    18841 acataaacat cgccgccgac acgaagccgt gcgaaatcat ctgaatgacc gcgcctttca
    18901 acgcccagtc gtccaactgc ccgtcaacaa acaaaaacat cccaagcgtt acaaaaccca
    18961 tatggctgat ggacgaatac gccaccagtt tcttcatatc ggtttgcacc agggccacca
    19021 taccgatata aattacggca atcaggctca atacgatgat cacgggggca aaatagcgag
    19081 ccgcgtccgg cataatcggc aggataaagc gcaagaaacc atacgcaccc agtttcagcg
    19141 taattgccgc caacaccatc gaaccgccgg tcggcgcttc aacgtgggca tccggcagcc
    19201 aagtgtgtac ggggaacatc ggcactttta cggcaaatga caggaagaac gctacaaaca
    19261 aaagctgttg tacgcccaac ggaatctgtt tgatgttttg gaaatcgaca atagagaagc
    19321 tgcctgtctg atagtacagg taaaccatgg caaccaacat caagagcgaa cccgtcagcg
    19381 tatagaggaa cagcttgacc gacgcataga cgcggcgcgg gccgccccat acaccgataa
    19441 tcaggtacag cggaatcagc ataccctcga agaacacata aaacagaatc gcatcctgcg
    19501 cggcaaacgc gccgttaatc aaacccgaca tcatcaggaa tgccgccata tactgcgccg
    19561 gacgtttctg aatgacttcc caaccggcca ataccaccag cagcgtaata aacgcattca
    19621 agatgataaa gagcactgaa ataccgtcca cgcccaatgc gtagttgatt ttcagaagcg
    19681 gaatccactc gtggaactcg gtaaattgat agccgccgct caaacggtcg aaaccggtaa
    19741 acaggggcag cgttaccaag aaaccggcaa gcgctcccat gaaggcaagc acacgcgcca
    19801 gcggcgcacg gctgtcttta ccggtcgcca aaaccagcac gcctgcggcg atgggtatcc
    19861 atattgccaa gctgagtagg tagttggaaa acatagtggt taacctgtgg ttaaaataaa
    19921 aaatatagtt gtatgggttg gtctttacag acagcgtttt aggaagtcgt ctgaaagaag
    19981 gaatgaatac tctcccccct tctttactta ttcccaatca atatctgtac gattgcgata
    20041 ttctgcctgt actaaaacat ctaattcagc caaatctttt atttctgact gcatagctag
    20101 taaagctctt aacttcatac ttaaatcaat atatttggag ccttcgcttg ctccttttgc
    20161 agacttaaat ttattttctt ctaaaaattt tttaatggga gcatttaaaa caggatatct
    20221 atctggatat tcttgacaaa gcatttcaga aaaaactgaa ccacgagtgg aaaccccaca
    20281 ttcttttagc caatcaattt gcttggcaac cacattatct ctctcagtaa gtggagcatc
    20341 aaaaactgat tggatggcaa tgcataattt tctaaaatct gaagacttac ccgtcctttt
    20401 ccacgtttta tcttgaaaac gattgcctac cccttctcct ttattttcgc ttttccaaga
    20461 ccataattta ttaaattccc cataaaaatc attattgtct atttttcctg ctgcacattg
    20521 cttaataaga ttttttaatt gattacacac agtttgatgg tttctcatct gctttctccg
    20581 tgccgcaatc agttcttgat tataatttgg catctccata aataacttat ccatcactga
    20641 ttgtctaaca gttgatttct tatagttaat ttcagcttcc tgatactcct caagccaatc
    20701 ttccgatacc gggatagatt tcatcgcaat ttcatcagcc caagatttga cttttataaa
    20761 atcctgctcg gaaattttag tcaaaatatt tgcttcataa ttgctattaa acgcagcatg
    20821 agtcaaattt gaagaaccga ttaaagcata atattcattc ttatcattct tccaaaatac
    20881 agctttagga tgaaaaccct gaatgccttg agctaccatc agtgtgaatt tcaaatggga
    20941 cggaagccat ttcaacactt tccgaatagc ctctttttta gacaatccga aatcttgtcc
    21001 aaaaataaat ttaaactctt gcaatttgct attgagctta ttttttacat cccaatgagt
    21061 gagatacgca gagaaaatat acagctctac tgcctctgaa acagctttct catatattga
    21121 aatcagttcc ttctcatttg gtgtatctga atgtaaaaat aactgtgtca tcccttttga
    21181 ctcctctata tttgttgaga ttgcctaaaa aatttaggaa acagataatt tgcatgttgg
    21241 ctatttcacg cctgttgggt accattttgt tccacccttt gacttaatgc ccacttctca
    21301 aacttttcag acgacctcta caaaactatg aacaggtcgt ctgaaactcc gcctttatct
    21361 gaacaagccc cagaaggtca tgccgagcaa taccaatacg ccgaacacca tagcggcggc
    21421 gtaggtgtag ataaagccgg tttggacttt acgcacttgc gcggcaattg cgccgaccag
    21481 tttggcggag ccgttgacga tgccgttgtc gataatggcg gtatcgccga ctttccagaa
    21541 gaagtttccc aatgcgcgcg tgcctttggc gaagacgttg aaatacaagg cgtcgaggta
    21601 gtatttgttt tcaaacaaaa cgtaaaccgg acggaacgcc tgcgcgattt tagcgggcag
    21661 gtgcggcagt ttgacgtaca aaagccatgc gctcaatacg cctgcggtag cgagatagag
    21721 gacgggcgaa tgcagactgt gcgacaccat cgccaatgcg ccgtggaact cttccttcat
    21781 gatgtgcatg gtcggatgcg cgtcggcgtt gacgaaaatc acgtctttga agaaatcgcc
    21841 gtagagcatg ggttcgatgg cgatgtagcc gatgatgacg gacggaatcg caagcagaat
    21901 cagcggcagg gtaacgacca gcgggctttc gtgcggattg tcgtttttgc ccaagccgtg
    21961 atgttcttca ccatggccgt ctgaatggtg ttcgggcaga ctgcgccatt tttcttcgcc
    22021 gtggaacacc ataaagtatt ggcggaacgc gtaaaacgcg gtaacgaaca cgctggcgag
    22081 gacggcaaaa taggcaaagc cgctgcccgg cagggtgctg tatttcactg cttcgataat
    22141 cgaatctttg gagtagaagc cggagaagaa cggcgtaccg atcagagaca aattgccgat
    22201 cagcatagtc agccaagtaa tcggcatgta ttttttcagg ttgcccatat ggcgcatatc
    22261 ttgatcgtgg tgcataccga taatcgcact gcccgccgcc aagaacagca aggctttaaa
    22321 gaaggcgtgg gtcatgacgt ggaacatcgc cacagaatag gcggacgcgc ccagagccac
    22381 ggtcatgtag cccagttgcg acagggtaga atacgcaacc acacgtttga tgtcgttttg
    22441 aatcacaccc aagaagccca taaacagggc ggtaatcgcg ccgattacca taatgaccga
    22501 cagcgcggtg ctgctcatct cataaatcgg cgacatacgc gacaccataa acaaaccggc
    22561 ggtaaccatc gtcgcggcgt gaatcaatgc agaaatcgga gtcgggcctt ccatcgaatc
    22621 gggcagccag acgtgcagcg ggaattgtgc cgatttaccc atcgcaccga caaacaggag
    22681 caaacaggtt acggtaatca aagaccattc cacgccgggg aaaagctgga tagtggcatt
    22741 ttgtacgttg ggcagatagg cgaatacgtc ctgataacgc aggctgccgc cgaaataggc
    22801 aagcaccaag ccgataccga gcaaaaagcc gaagtcgccg acacggttga tcaaaaaggc
    22861 tttcaggttg gcaaatgtcg cgcccgggcg tttgaaatag aaaccgatca agagatacga
    22921 caccaagccc accgcttccc aaccgaagaa gagctggatg aagttgttgc tcataatcag
    22981 catcaacatg ctgaatgtaa acaaagaaat atagctgaag aagcgttggt agccgacttt
    23041 ttcatcgtgc atataaccga tggtatagat atgcaccatc aacgacacgc ccgttaccac
    23101 gaccatcatc atcgccgtca tcgtatcgac caagaagccg acggagaaat ccaaaccgcc
    23161 cattgtcagc caggtataga cgttttcatc aaacttggca cggctgccat tgaggaatcc
    23221 ccacagcaca taagccgaca gcacggcaga tacagcgaca ccgagtatcg taaccgtatg
    23281 cgcgccggca cgcccgattt tgttgccgaa caaacccgca atcaacgagc ctgccaacgg
    23341 aacaagggca attatcaaat ataaagtcat atcgttcata attacccttt caactcgtcc
    23401 aaatcggcaa cattaatcgt ttgtcggttg cggtacacca gcaccataat cgccaaaccg
    23461 atggcggatt cggcagcggc aacggtcaat acgaagaata cgaaaatttg tccggcagta
    23521 tcgcccaaat gttgcgagaa ggcgataaag ttgaagttca ccgccaaaag catcagctcg
    23581 atggacatca gcaataccag cacgtttttg cggttcataa agatgcccat cgcgctgata
    23641 ccgaacagga gagcgcccaa taccagataa tgcgtcaaag taatcatgct ttgccctccc
    23701 cttccgtctt gaggccgtct gaaacttcgc tttcttcggc agattcgact tgcggtttga
    23761 ccgcttccat tttcaccaga cgcatacggc cttgatcggc gcgtacttta acttggtcgg
    23821 ctggatccat acgtttcgga ttggtggttt tgcggtgaac caatgcaatc gcggccacca
    23881 tgcccaacag cagcaatacc gccgccaatt caaacggcaa caggtagtcg gtataaatac
    23941 ggctgcccaa atcgcggata ttgttgtaat cggccggaat gtctttcatc agaccaaatg
    24001 cggccaggtc ggttttcggg ttgaccagaa tcaggatcag ggcaaccgcc aacaacgtac
    24061 cgaccacacc ggcaacaggc gcgtgccgcc agaaaccggc gcgcatttcc tcgatgtcga
    24121 tattcagcat catcacgacg aacaggaaca acaccattac ggcgccgacg taaaccacca
    24181 ccagcgtaac gcccaaaaac tccgcctgca tcagcatcca aatcatcgcg ctcacgcaga
    24241 aggtcagcac cagatgcaaa gcggcatgga cagggttttt ggcggtaacg gtacgaaccg
    24301 caccgtacaa aacgatggcg gcaaggatat agaacagaat cacggaaaaa gtcattcgtc
    24361 tgctccttag cggtacggcg cgtcagcggc tttgcgtttg gcgatttcgg cttcgtattt
    24421 gtcgccgatt gccaagagga tgggcttggt catatgcaag tcgccttttt tctcgccgtg
    24481 gtattcaaaa atatgggttt ccacaatcgc atcggtcggg caggcctctt cgcagaaacc
    24541 gcagaagatg cacttggtca ggtcgatgtc gtaacgcttg gtgcggcggg tgccgtcttc
    24601 gcgttcttcc gattcgatgt tgatcgccat tgccggacac accgcctcgc acaatttaca
    24661 cgcgatgcag cgttcctctc cgttcggata acggcgttgc gcgtgcagac cgcggaaacg
    24721 cacggattgc ggcgttttct cttcgggaaa ataaattgtg tctttgcggg caaaaaagtt
    24781 tttgagcgtt acgcccatgc ctttgaccag ttcgccaagc agaaaggttt ttactaagtt
    24841 agccatatta tgttccctca aaacagggat ttcgttaggt attcaaaatc gctttgttca
    24901 gacggcctca agatgccgtc tgaaacttat ttccacaaat tcagcggtga aatcatccac
    24961 acgcccaaaa ccacgatgta ggcgaagccg atcggaatca gcactttcca gcccaaacgc
    25021 atgatttggt cgtagcggta gcgtgggaag gttgcacgaa tccacagata ccagtacagc
    25081 accgccgcca ttttcacgaa catccagaat gcggaaggcg taccgacaat gccccagctt
    25141 tgcgggaacg gagacaacca gccgccgagg aacatcagcg atgtcagcgc ggcaatcaga
    25201 atcatgaaaa tgtattcggc aaggaagaac agcgcgaatg caaagccgga gtattcgacg
    25261 tggtgtccgg caacgatttc agactcgccc tctgccacgt caaacggtgc gcggttggtt
    25321 tcggcaacgg cggaaatcag atagacgatg aagattggga acagcggcag ccagttccac
    25381 gagaacacag aaccgcccgc gatgcctttt gcctgcgcgg caacgatgtc ggagaagttc
    25441 atgctgcccg ataccatcac aacgcacacc agcgcggcac tcatggcaat ctcgtaggaa
    25501 atgctttgcg cggaagcacg cattgcaccc aagaacgaat atttggagtt ggaagcccag
    25561 cccgcgataa tcacgccgta aaccgacagc gaggtaatca tcaggatgta caaaaggccg
    25621 atattgatat tggtcagcac ccattcttcg ttaaacggaa tcaccgccca cgccgcgaaa
    25681 gacggggcga gcgacataat cgggccgatg tagaataagg ctttgtttga cagcttcgga
    25741 cgggttacct ctttaaacaa gagtttgaac acgtcggcaa acggctgaat cagaccctgc
    25801 gggccggtta cgttcgggcc gacgcgaagc tgcataaagc cgatgacttt acgttcgaaa
    25861 tacgtcaggt aggcaacggt cagaatcagc ggaatcagga taatcacgat tttgacgatg
    25921 acggatacca ccaagcctac ggtgatgccc aaatcgccca gaccgagcgt tgcggcaaag
    25981 aggttttgga accattcctg cataatcaag ctcccgccag ttcaatagtg cccatcaacg
    26041 cacccagcgc ggcattttcg gtatgcagcg gcagatgcac cacgttttca ggcaaaccgg
    26101 catcggcttt gacggcaacc gatacgcttg cgccgttttg tttggcgaca gcggtttgtc
    26161 cgtcttgcag gcccaagcgt gccaatgtgt tcggatttac acgcgcggca ggcacggcgg
    26221 catggctggt ttcttgcaac ggtgcggaac ggcgcacgat agaatcagta tgataaatac
    26281 cgacgccgcc gacacggacg aggcggtctg aggccgtctg aacgccctcc cctgcccatg
    26341 tgctgcggtt gtccagtttg gacggcaggc tttccgcatc cagcgcgtct ttcagaatcg
    26401 cggcggtatc gtggtattca aaacctttca ggtcaaacag gttgcccaat acgcgcaaca
    26461 ctttccacaa cggacgtgaa tcgccgaagc cttgtaccac gccgtggaaa gattgcagac
    26521 ggccttccat attgatgaag ctgcctgatg tttcggtaaa cggcgcaatc ggcagcaata
    26581 cgtcgcacac gtccagcagc gtttcgctga caaacggcgt aaacgccatc acgcttttcg
    26641 cctgtttcaa cgcggctacg gcttttgcac cgtcaaccgt atcgatttca ggctcgacgt
    26701 tgagcagcaa gactgcctgt ttcggcgtgt ttgccatttc ggcaacgctc tcgcccgagt
    26761 tcacacccaa tacgtccgca ccgacgctgt tggcggcttg cggcaaaatg cccagcactg
    26821 cgccggtcgc gtccgccaac tcttgcgcgg cggcataaat ggcggcgtaa tcaggatggt
    26881 tttgcacttc cgcacccaaa atcaccgctg ctttttcagc gtttttcagg ctggcggtaa
    26941 cggcatgttc cgcatcggca gacaggtttt tcagacggcc tgcccactcg tcgggatgtg
    27001 cggcttcttg agacagaagc ggcataaaca attcttcttt actgccggcc aatacgctca
    27061 aggccatacg gtctttggcg gcgcggcgca ggcgggcggt caggagcggc tgttctttgc
    27121 gcaagttcgc accgactacc aatacggcat cgttgtcagc caaagattca atactttgtc
    27181 ccaaccattg cgcaccttta aggccgtctg aaagacgttt gtcttgttgg cgcaaacggg
    27241 ttgcaaagtt tttaacaccc aagccgtcgg caaatttttt ggccagatac agctcttcaa
    27301 ccgtattcat cggattggcc caaacaccga cttggttttg gttgccgtct ttggcgatac
    27361 attcaatcgc gctgcggaca tattccaacg cggttttcca atccacgtcc atccactcgc
    27421 caccctgttt gattttcgga tttttcagac ggctttcgtg atacaggcct tcgtaggcaa
    27481 aacggtcgcg gtcggacagc cagcattcgt tgatggcttc gttttccaac ggcaacacgc
    27541 ggcggacggt atggtctttg gtttgtacaa tcaggttgct gcccaaagcg tcgtgtgcgg
    27601 aaacggattt gcggcggttc aattcccaag tacgcgcgtt gaagcggaac ggtttgctgg
    27661 tcaatgcgcc gacgggacac aaatcgatga cgttgcccga cagctcggtt tccaccgctt
    27721 tgccgataaa gggcatgatt tcggagtgtt caccacgatt caccatcgca atttcctgca
    27781 aaccggcgat ttcttcggtg aaacgtacgc agcgggtaca gtggatacag cggctcattt
    27841 cctcggcgga aaccaaagga cccatgtcct tgccgacgac ggaacgtttt tcttcggtat
    27901 aacggctggt ggttttgccg tagcccaccg ccaaatcctg caactggcat tcgccgccct
    27961 ggtcgcaggt cggacaatca agcggatggt tgatgagcag gaactccatc acgccttctt
    28021 gcgcctctcg ggcttttgcc gaatgcgtac gcacaatcat gccgtccgtt accggcgtgg
    28081 cacaggcagg cagcggtttg ggggcttttt ccacatccac cagacacata cggcagttgg
    28141 cggcaataga gagctttttg tggtaacaga aatgcggaat ataagtaccg agcttgtgcg
    28201 cggcttcaat taccgtcgcg ccctgctcca cagatacctg tttgccgtcg atttcgattt
    28261 gtaacatggt tcgttcctag ttacgggtat ttgataaata attttttatg gagatcgtct
    28321 gaaaaatggg cttcagacgg ccttttgagt ttcattgaca atcaatactg tttaaaacgt
    28381 ttactgccac cttgccgtca ttcccgcaca ggcgggaatc cattttttg
//
LOCUS       DAWTCI010000017        27856 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWTCI010000017 DAWTCI010000000
VERSION     DAWTCI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27856
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.017"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(85..2127)
                     /gene="recG"
                     /locus_tag="WM481_000770"
     CDS             complement(85..2127)
                     /gene="recG"
                     /locus_tag="WM481_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFA9080132.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLGRLKLPRLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRVSGTAAALRGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTHPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVCLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLSIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPEPNIG
                     LVHGRMKAAEKAEVMAEFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAGESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(2213..2656)
                     /gene="secB"
                     /locus_tag="WM481_000771"
     CDS             complement(2213..2656)
                     /gene="secB"
                     /locus_tag="WM481_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFA9080133.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGDPEVDMRVST
                     GSQKLEDGYYDVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="WM481_000772"
     CDS             complement(2677..2934)
                     /gene="grxC"
                     /locus_tag="WM481_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFA9080134.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQRLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            3153..4646
                     /gene="rng"
                     /locus_tag="WM481_000773"
     CDS             3153..4646
                     /gene="rng"
                     /locus_tag="WM481_000773"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFA9080135.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSGHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(4681..5931)
                     /locus_tag="WM481_000774"
     CDS             complement(4681..5931)
                     /locus_tag="WM481_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HFA9080136.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVGPDSKSET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLRADGDDDYYRAYIRTTEQGRIAV
                     MQENEYREDLAEDAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRING
                     LPALSVYNIPSEIRGFVTAINLLLKRVDEDIRRRQRFVADAAHELRTPMTALSLQAER
                     LNNMPLPPDAGRQSAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLVLEKRQDIGVAVGGDVEVSADETEIYTLVKTFADNAVRYTPNGGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESECARVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(6013..6642)
                     /locus_tag="WM481_000775"
     CDS             complement(6013..6642)
                     /locus_tag="WM481_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFA9080137.1"
                     /translation="MSASLKDGGYAVDWVKNGAQVAAAAAAQPYDLMLLDLGLPGRDG
                     LDVLSEIRAAGCTVPVLIVTARDDLYSRLNGLDGGADDYIVKPFDMAEFKARMRAVLR
                     RGSGQAQACLSNGALSLNPATYQVEIIAEGRQVALSNQEFSVLQALLARPGVILSRSD
                     SEDKVYGWGGEVESNAVDFLIHGLCKKLGKESIQNVRGVGWLMPRQDAV"
     gene            complement(6910..8223)
                     /locus_tag="WM481_000776"
     CDS             complement(6910..8223)
                     /locus_tag="WM481_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFA9080138.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLIGALLAGFDVS
                     QLKEFYSGGTKSVTQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLSVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPRGGPI
                     GRVASVLGADVGELYKPLLTVQIIGVVFILVLSLFLGVREKRRIVRELGALPAVADLI
                     KPAPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILISSIAAGIVPLP"
     gene            complement(8601..9173)
                     /locus_tag="WM481_000777"
     CDS             complement(8601..9173)
                     /locus_tag="WM481_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFA9080139.1"
                     /translation="MIVAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGH
                     LPLYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGS
                     KPNADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLF
                     DENGKLIDSARDFVQSYINQFVGLIERNAK"
     gene            9332..10741
                     /gene="dacB"
                     /locus_tag="WM481_000778"
     CDS             9332..10741
                     /gene="dacB"
                     /locus_tag="WM481_000778"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFA9080140.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRAGIPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLRDKGIRNITGRLMLDHSLWGEVGSPDHFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFAQNNLKITASQAACPSVKKLMRASF
                     SGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNRWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            complement(10806..11975)
                     /locus_tag="WM481_000779"
     CDS             complement(10806..11975)
                     /locus_tag="WM481_000779"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFA9080141.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPAISHEELSKAVIEQIIKPVLPP
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     CEHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAASLKAAAGL"
     regulatory      complement(12158..12268)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            12321..13214
                     /locus_tag="WM481_000780"
     CDS             12321..13214
                     /locus_tag="WM481_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFA9080142.1"
                     /translation="MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTGIQGVKQIIKALRAGEATIILPDHVPSPQEGGGVWADFFGKP
                     AYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNT
                     EYWIRRFPTQYLFMYNRYKTP"
     gene            complement(13259..14323)
                     /gene="tsaD"
                     /locus_tag="WM481_000781"
     CDS             complement(13259..14323)
                     /gene="tsaD"
                     /locus_tag="WM481_000781"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFA9080143.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRSHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYELLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNNICRAFQDAVVEVLEAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPMAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWSLSEIVK"
     gene            complement(14439..15626)
                     /gene="ccsB"
                     /locus_tag="WM481_000782"
     CDS             complement(14439..15626)
                     /gene="ccsB"
                     /locus_tag="WM481_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFA9080144.1"
                     /translation="MTEHYKTLPEHELLIQKSLIRNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAVQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(15619..17571)
                     /locus_tag="WM481_000783"
     CDS             complement(15619..17571)
                     /locus_tag="WM481_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HFA9080145.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWTRIFDF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVRGFQGKTVSREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLAGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGMLVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLRDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPILQDKDYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKGQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWRQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVFMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(17849..18472)
                     /locus_tag="WM481_000784"
     CDS             complement(17849..18472)
                     /locus_tag="WM481_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HFA9080146.1"
                     /translation="MRRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            18679..19308
                     /locus_tag="WM481_000785"
     CDS             18679..19308
                     /locus_tag="WM481_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HFA9080147.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLRH
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(19429..21825)
                     /locus_tag="WM481_000786"
     CDS             complement(19429..21825)
                     /locus_tag="WM481_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFA9080148.1"
                     /translation="MIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYE
                     KYGEDAYTQGFKVYTTVRTDHQKAATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVALDRRALGFAARAVDNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRPSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRAGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLM
                     LDNSGIAPQPSRRAKEDDEAAVENEQQGRSDETRQDVQETPVLPSNTDSKQQQLDSLF
                     "
     gene            21977..23092
                     /locus_tag="WM481_000787"
     CDS             21977..23092
                     /locus_tag="WM481_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFA9080149.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIVASVPQ
                     NLATIEQLTYTAKDAELDLQGFVESSISEASSISLEEANYDYQVLSQSAVGEAVLSVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELADEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSAEQLNQLIQRTYQVTAEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAVRQKGIAQTVASQTNADVQ
                     CVHPARYFANDLKTDEQQFELDAPTLTKAFGLAVRGL"
     gene            23095..23694
                     /locus_tag="WM481_000788"
     CDS             23095..23694
                     /locus_tag="WM481_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFA9080150.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINKQSEKNTLLEISIAHLDTELSEIQKLKQEKDAFLIKKNKIGELQIKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            23695..24342
                     /gene="pilO"
                     /locus_tag="WM481_000789"
     CDS             23695..24342
                     /gene="pilO"
                     /locus_tag="WM481_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFA9080151.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLVVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKKLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            24360..24905
                     /locus_tag="WM481_000790"
     CDS             24360..24905
                     /locus_tag="WM481_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFA9080152.1"
                     /translation="MKHYALLISFLALSACSQSSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETAKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIILNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPVAEQN"
     gene            24924..27119
                     /locus_tag="WM481_000791"
     CDS             24924..27119
                     /locus_tag="WM481_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFA9080153.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPFTESVV
                     SVSAPFSPAKQQAAASAKQQAATPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQ
                     HDHIIVTLKNHTLPTALQRSLDVADFKTPVQKVTLKRLNNDTQLIITTTGNWELVNKS
                     AAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNI
                     VASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQ
                     AEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATN
                     TLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRDLGVKFGATGRKKLK
                     NETSAFGWGVNSGFGGGNKWEAQTKINLPVAAAANSISLVRAISSGALNLELSASESL
                     SKTKTLANPRVLTQNRKEAKIESGYEIPFTVTTASGGGNSTNTELKKAVLGLTVTPNI
                     TPDGQIIMTVKINKDSPAQCASGSNTILCISTKSLNTQAMVENGGTLIVGGIYEENNG
                     NTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIIDTAGNSLRY"
BASE COUNT         7067 a         8039 c         6692 g         6058 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaaaatca gatttccgtt acactttttt
       61 ctaatatttc agacggcatt ttgctcacac acccaaataa ccttccctgc cggaaagcca
      121 ccttgccaaa tgcgcttcga cgatttcagg gttttgttca atcagcatcg gggcggtttc
      181 gcgcgcttgt tccaaaaggt gtaagtcttc ttcgagcttg gcgaagcgca gcataggcac
      241 gccgctttgg cgcgcgccga gaaattcgcc ggggccgcgg atattgaggt cttggcgggc
      301 gatttcgaag ccgtcggtgt gttcgtagat gactttcagc cgcgctttgg cgagttcgcc
      361 caagggttcg gcaaacagga ggacgcacac gctttccccc gcgccgcgcc ctacccgtcc
      421 gcgtaattgg tgaagctgcg ccaagcccat gcgctcggcg tgttcgatga ccatcagggc
      481 ggcattgggc acatctacgc cgacttcgat aacggtggtg gcgaccaaga cgttcagacg
      541 gcctgcggca aattccgcca tgacttcggc tttttcggcg gccttcatgc gcccgtgtac
      601 cagtccgata ttgggttcgg gcaatgccgt ctgaagccgg gcgagggttt cggcggcggt
      661 ttgcagttgc agggtttcgc tttcttcaat caatgggcag acccaatatg cctgccgccc
      721 ttttcggcaa gtgccgagga cgaagccttc gacttcggcg cggcggacgt tgttgacgag
      781 gcgcgtttta atcggagtgc gcccgggcgg caattcgtcg atgacggata cgtccaagtc
      841 agcgaagaaa ctcatggcga gcgtgcgcgg gatgggtgtg gcggacatca tcagctgatg
      901 gacttcgcgc cctttgtttt tgagtgcgag gcgttgtgca acgccgaaac ggtgctgttc
      961 gtccacaatg ctcaagccca aattgtgaaa cgccacgccg tctgaaaaca gggcgtgcgt
     1021 gccgacggcg attttgacgc tgccgtcggc gagtttggct ttggcttcgt ctttggcttt
     1081 tttgcgcaaa ctgccaaaaa ggcagacaac ttcaatgccc aaaggttcga gccattgttt
     1141 gaacttgatg aagtgctgtt cggcaaggat ttcagtgggc gccattacag ccacttgcgc
     1201 gcctgattcg atggcggtca gcgcggataa ggcggcgacg atggttttgc cgctgccgac
     1261 atcgccttgc agcaggcggt gcatggggtg ggtttgcgcc atatcgcggc agatttcgga
     1321 aacaactttt tcttgtgcat cggtcagggc aaacggcagg gcttggcgca gggcttgggt
     1381 caatgtgccg tcgccgcgca atgccgccgc cgtgccgctg acgcgcttct gccgcgccaa
     1441 acgcatggac aactgttgcg ccaaaagttc gtcgaatttg agccgctgcc acgccggcag
     1501 cgtgccgtct gaaagttgat gaatagtgaa actcggcggc ggcgaatgca aaaggcgcag
     1561 gctttcggca aggcgcggca gcttcagacg gcctaataaa gcatcaggca aggtgtcgtg
     1621 cagcggcgta acgtccaacg ccgtctgaat gatgcggcgc aaagtgggct ggttcaaacc
     1681 gtttacggtc gggtaaaccg gcgtgaggct ttccgccaaa ccgccgccct cggcatcgcg
     1741 gattttggga tgaatcatct cgtcgccgta aaatccgtgt ttgatttcgc ccacggcgcg
     1801 gatgcgtttg ccgaccgccg tctgcttctg atggctggcg taaaagtgga tgaagcgcag
     1861 aaaaaggacg ctgccggaac cgtcggcaat ttgaacaatc agctgcttgc gcggtttgaa
     1921 cgttacttcc tgatggataa cctcgccctc gacctgacac ggcacgccaa tcggcgcgtc
     1981 cttaatcggc ataatgtgcg tctcgtcctc gtaacgcagc ggcaggtgca acaccaaatc
     2041 ccacgcggta tggaggttga gtttgtcgag cttcttggcg gaaacatcgg tgattttgag
     2101 ctgtttctgg gtttcgggcg acatcatggg cagattcctt tggacgcgcc tattttatcc
     2161 gaaaacaaaa atgccgtctg aaacggattc agacggcatc ggcaggcagg aatcaagccc
     2221 cggcggcttc ggcttcctgc tgttgttggt aaatcgcttc aaaattaatc ggcgcaagca
     2281 ggacgggcgg gaagccggcg cgcgttaccg taccggaaac cgcttcgcgc gcgtaaggga
     2341 agaggatgtt cggacacgcc acgcccaaca gcagctgcac atcttcttcg gggatgtttt
     2401 ccagacggaa aataccgctt tgggttactt cgttcaaaaa catcgtgcgc tcgttatcca
     2461 atttggcggt aacggttacg gtaacgtcca cgtcgtagta gccgtcttcc agcttttgac
     2521 tgccggtgga aacgcgcata tccacttcgg gatcgccctg ttccaaaaag atttgcggcg
     2581 cgtgcggcac ttccaaagac aagtctttga catacagtcg ctcgatgctg aatacgggtt
     2641 gcagttcttc gctcattttg ttttcctagt tgtgggttaa gggttcagca gtccgtccag
     2701 cccgccttcc tgctgaaggc ggtagaggtc ggtaaatccg ccgacgtgcg tttcgccgat
     2761 gaaaatctgc ggcacgctgc gctgtcccga aagccgctgc atttcggcaa aggcttcggg
     2821 gcttgcatcg acacggattt cgtcgatatg tccgacacct gccgcgtgca gcagcctttt
     2881 cgccatcgcg cagtaggggc aaaacggacc tgtgtacatg gtaacggtct gcatattggt
     2941 tttccgaaag ttttgcaatg ataatcaata taggggcaat cgctccgttt ggcaagtgcg
     3001 gaacagatgc acgttcagac ggcatgtgcg gagtgtgtca aagtttcttt tttaaagtat
     3061 gataggcatt gtgaaaaata tttttgcacc cgctctgcgc ggcggggcgg aacggatgca
     3121 aaaatatttt tattacattt tcaggaaaaa ccatgttgtc aggactcccc atccccaaag
     3181 acatcgcgcg cccgcccgaa acgatattgg tcaacatcac gccgcaggaa acgcgcgtgg
     3241 cggtgttgga ggaaaacaat atctgcgagc tgcacatcga gcgcaacagc gggcacagcc
     3301 tggtcggcaa tatctatctg ggcgtggtgc gccgcgtgct gcccgggatg cagagcgcgt
     3361 ttatcgacat cggcttggaa cgcgcggcgt ttttacacat cgtcgatgtc ctcgaacaac
     3421 gccgcaaccc cgaagaaacc cagcgcatcg aacatatgct gtttgaaggg cagtcggttt
     3481 tggtgcaggt catcaaagac ccgatcaaca ccaaaggcgc gcggctttcc acccaaatct
     3541 cgctggcggg gcgtttcctc gtccaccttc cgcaagaaga ccacatcggc gtgtcccaac
     3601 gcatcgaaga cgatgccgaa cgcagcagcc tgcgcgaacg cctcgacaag ctcctgccgg
     3661 aaaatgcctg ccggggctac atcatccgca ccaacgccga aaacgccacc gacgaacagc
     3721 tccagtccga catcgactac ctgaccaaag tgtgggaaca catccaagaa caggcgaaaa
     3781 tccggccgcc ggaaaccctg ctttatcagg atttgccttt aagcctgcgc gtgttgcgcg
     3841 atatggtcgg ctgcgacacg cagaaaatcc tcgtcgattc caccgtaaac cacgggcgca
     3901 tgacgcgttt tgccgaacaa tacgtccacg gcgcattggg caggatagaa ttgttcaaag
     3961 gcgaacgccc gctgtttgaa acccacaaca tcgaacagga aatcagccgc gccctgcaac
     4021 cgcgcgtcaa cctcaacttc ggcagctacc tgattatcga gtccaccgaa gccatgacca
     4081 ccatcgatgt caacaccggc ggcttcgtcg gcgcgcgcaa tttcgacgaa accatcttcc
     4141 gcaccaacct cgaagcctgc cacaccatcg cccgcgaatt aaggctgcgc aaccttggcg
     4201 gcatcatcat catcgacttc atcgatatgg cgcaggaaag ccaccgcgaa gccgtgttgc
     4261 aggagcttgc caaagccctc gccttcgacc gtacccgcgt taccctgcac ggttttacca
     4321 gcctggggct ggtcgagctg acgcgcaaac gctcgcgcga aaacttaaac caagtcctct
     4381 gcgaaccctg cccttcctgc caaggcaggg gccgcctgaa aacgccgcaa accgtatgct
     4441 acgaaatcca gcgcgaaatc gtccgcgaag cgcgccgtta cgatgccgaa agtttccgca
     4501 tcctcgccgc ccccaacgtc atcgatttgt ttttggacga agaatcgcaa tccttggcaa
     4561 tgctgataga tttcatcggc aaaccgattt ctttggcggt cgaaaccgct tacacgcagg
     4621 aacaatacga tattgtgctg atgtaaaaaa tgccgtctga agccttcaga cggcatctgt
     4681 ctatttcagg gtttccttgt ccaacagcgc gcgtatcaac agcccgtgtc cgaaacgtcg
     4741 gctgtcggtc agttccaaat atccgccgta ttttttggcc agcgtgtcgg caatcgacag
     4801 ccccagcccc gccccctgct gctccgttcc caaaatacgg taaaacggat cgaggacgcg
     4861 ggcgcattcg gattcgggaa tgccgttccc gttatcctcc acccacacgg cgagatattt
     4921 cccttcgtcc gtgaaaccca aatctatcct gcccccgttc ggcgtgtaac gtaccgcgtt
     4981 gtcggcaaag gttttaacca gcgtatagat ttccgtttcg tcggcagaca cttcgacatc
     5041 gcctccgacc gccacaccga tgtcctgacg tttttccaaa accagcggca tcagttcctg
     5101 caacacttgg cggaaacggc tttgcagacc gaatgtcgtt ttcgtcaaag gggtttcgtc
     5161 cgactgcgaa cgcgccagtg ccaaaagctg ttcgagcagg tgtttgttgc gcctgatgct
     5221 ctgctgcaaa acggcggact gccgccccgc atcgggtggg agcggcatat tgttgagccg
     5281 ttccgcctga agggaaaggg cagtcatcgg cgtacgcaat tcgtgtgccg cgtcggcgac
     5341 aaaccgctga cggcggcgta tatcttcatc aacacgtttc aaaagcagat tgatggcggt
     5401 tacgaaccct ctgatttcac tgggaatatt gtacacactc aaagcaggca ggccgttgat
     5461 tcggcgttgt tcgagacttt gcgacaattt gcggacgggg cgcatggctt tgtgcgtaat
     5521 ccacacagtc agcagtatca tcagcggcag tgccgccaac aggggcaaca cgctttgccg
     5581 tgccgcatcc tccgccaaat cttcacggta ttcgttttcc tgcatgacgg caatccgtcc
     5641 ctgttcggtc gtgcggatat aggcgcggta ataatcgtcg tccccgtccg cccgaagcgt
     5701 gtgcagaccg tccgccagat gcgcgggcag gctgacaaca gggtcttcct gctgcggcat
     5761 ctgtaccaaa atacgcgtat cgccgtcgcc ttcgggcaaa gtttcggatt tggaatcagg
     5821 gccgacatac aatgccgcct gacggagcag gtcgtcctgc aacgcttccg tttcgtggaa
     5881 ggtttcgtag taggaaaaca tacctgcaag cattgccagc ggaacaaaca tccaaatcag
     5941 ggcaaggctg attctgacct gaagcgaatg ttttaaaatt ttaaaaaaac ggtccggcat
     6001 cttcgttcct gttcagacgg catcttgacg cggcatcagc cagccgacac cgcgcacatt
     6061 ttgtatgctt tccttaccca atttcttgca cagcccgtga atcagaaaat ccaccgcatt
     6121 gctttcgact tccccgcccc aaccgtaaac cttgtcctcc gaatccgagc gcgacaaaat
     6181 cacacccggc ctcgccagca aagcctgcaa taccgaaaac tcctggttgc tcaatgccac
     6241 ctgcctcccc tcggcaataa tttctacctg atacgttgca ggattgaggg ataacgcacc
     6301 atttgacaga cacgcctgcg cctgtccgct cccgcgccgc aacaccgccc gcatccgcgc
     6361 cttaaactcc gccatatcga acggcttgac gatatagtca tccgcaccgc cgtccaaacc
     6421 gttcagccgg ctgtacaaat catcccgcgc cgtaacgatc agcacgggga cggtacagcc
     6481 tgccgcgcgt atttccgaca aaacatccaa accgtcccgc ccaggcaaac ccaaatccag
     6541 cagcatcaag tcataaggct gggcagcggc agccgccgca acctgcgcgc cgtttttgac
     6601 ccaatccacc gcatagccgc cgtctttcaa acttgccgac accgcttacg caatcatcgc
     6661 attgtcttcc accagcaaaa cacgcatcaa ctttcccttc aaaataaacc gtgcctattc
     6721 taacacccca aaattagccg caatttagcg gcctttacgc ttgccggtat ttttcaaaac
     6781 tgcggcacaa aaaaaccgcg ccggcaactg ccttcagacg gcattggggc gcgattgcaa
     6841 cacacggaca ggcaggcaaa gcctgcgaca gatcacagga acgattcggg cttcaggcgg
     6901 cttcgccgtt tacggcagag gcacgattcc tgccgctatc gaactgatca atatcgccag
     6961 cgacaaaccc cacgcccaga aaaacgaata acggatgtgt ttgcccatcg acaatttcgc
     7021 caaacccaaa cccatccaca aagccggcga aagcggcgta acaaaagtac cgacgatact
     7081 gccgatcaac atcgcatagc ctgccgcttc gggtgcaacg cccgcctgcg aggtaatctg
     7141 ttccacaatc ggaaacagtc cgaaataata agcgtccgta ctcaaaacca actcaagcgg
     7201 aatacccaac acaccgatgg caatatgcag ataaggcagc aacgcgtccg gcaggatatg
     7261 cacaaggtct ttggaaatcg cgtccaacat ccccgcgccc ttcaaaatcc ccaaaaacgt
     7321 acctgccgcc aaaataatgg acgccatcat caccgcgccg ccggcgtggg cataaatccg
     7381 ctccatctgt tcctgcgggc tgcggtaatt caaaagcaac gccgccgttg cagccagcat
     7441 aaatacataa cccggcggga agatgcccga aaaaagcagg ctcatcgccg ccaaaaacag
     7501 caggacattc caccaaaaca gtttcggacg cgccaatttt tgttcttctt ccgacaaagg
     7561 cgccggcttt atcaaatccg ccacggcggg caacgcgccc aactcccgga caatccgcct
     7621 tttttcacgc acacccaaaa acagggacag cacaaggata aacaccacac cgataatttg
     7681 caccgtcaac aaaggtttat acaattcgcc cacatctgcg cccaacacgc ttgcaacccg
     7741 cccgatcggc ccgccccgcg gcaaaaggtt gattagcccc gcgctggaag tcagcagcaa
     7801 aaacagcagg taaggattca tatgcagacg cttgtaaagc ggcaaaaggg cggggacgac
     7861 cgataaaaac gtcgtcgcgc ccgccccgtc caactgtgcc accaccgaca ccaagaccgt
     7921 ccccacactc actgccacga tattaccccg agtcagctta atcaaaccgc ctatcatcgg
     7981 acggaacagc cccacatcgt tcatgattcc aaaaaacaaa atggaaaaca taaacataat
     8041 cacaatctgc gtcaccgatt tcgtgccgcc cgaataaaat tcttttaatt gggatacatc
     8101 aaaccccgcc agcaacgccc caatcagcgg caccaagatt aatgcgatga tgggcgacac
     8161 tttttccgtc agcaacagcc atacgatgac cccgataatc agcaatccga taaacgtcag
     8221 cattatttct cctttatttt attttgaaca taaaaccgcc cgtgcaggca aaaccgccaa
     8281 catatgcggg ataagccttg cattccattc acgcctgccg acatttctgc atccgtccgt
     8341 catttcacgg cggcaaccga tacggaacaa ccggcaaacc ggtatcggga cggcgcgggg
     8401 gcatccgtcc cggtgcaccg attcaaacga aactgccccc tatcattgcg gagcgcgggg
     8461 cgtgccgtac acgcgggatt ttaaacacgc cgatcatttg ccgcgcgtta tccggcaaac
     8521 ggcaaacctt gacgctgccc ggcccatata aaaaagccgc aaaacccgaa ccggttttgc
     8581 ggcttttcga tttggtttgt ttatttggca tttctttcaa tcaaaccgac aaactgattg
     8641 atatatgact gaacaaaatc ccttgccgag tcaatcaact taccgttttc atcaaacagc
     8701 gtcggcgaat tgcccaaaaa tacttccggc tgtccggtta cgggcatatc aaaatacgac
     8761 agggcaaggc gcaggttttt ttgggaactg taaccgccca tcttgccgac ggaatggctg
     8821 atgatgccgg ccggtttgtt tttccaagcc acgtcggcat tcggtttcga gccgatgtcc
     8881 accgcatttt tcaaacaggc gggaatggtg cggttgtttt cggacgtaac gaacaaaatg
     8941 ccgtccgaag ccttaatcgt ttcgcggaaa gccgtgtagc tttcgggcag cggcacatct
     9001 tccaccgcag ggtcgtcata atcgaaattg taaagcggca gatgtccgat ttcgacgatt
     9061 tccgcctgcc agccttcggg gaacatctcc gccgcattca atgccacttt gcgcgcaaac
     9121 gaagcacggc gcaggctgcc caccaaaata ctgattttct tagccacaat cattcctcct
     9181 gaatattaag tttgtgcgtt tcaatcattt cataatggta acgattatta tatatgtgat
     9241 ttcccctgca aacaagccgg tccgccgccc cggcgttccc acttatccgg ctttgcctta
     9301 taattgcttt ttttatgtaa cagattcacc tatgaatttc cccaaaacag cggcctccct
     9361 gctgctgctt ctcgcctccc tcgccgcaca cgcgctcgat accggccgca ttccgcaaaa
     9421 cgaaatcgct gtatatgtcc aagagcttga cagcggaaaa gtcatcattg accaccgtgc
     9481 cggcataccc gtcaatcccg cgtccacgat gaagctcgtt accgcgtttg ccgccttcaa
     9541 aaccttcggc agcaattacc gctgggcgac cgagtttaaa agcaacggta cggtaaacga
     9601 cggcacgctt gacggaaacc tgtattgggc gggcagcggc gaccccgttt tcaatcagga
     9661 aaacctgctt gccgtccaac gccagttgcg cgacaaaggc atccgcaata tcacggggcg
     9721 cctgatgctc gaccacagcc tgtggggcga agtcggcagt cccgaccatt ttgaagccga
     9781 cagcggttcg ccgtttatga cgcccccaaa tccgactatg ctgtctgccg gtatggttat
     9841 ggtgcgcgcc gaacgcaatg ccgccggcag taccgacatc ctcaccgatc cgcctttgcc
     9901 gcatattttt gcccaaaaca acttgaaaat taccgcctcc caagctgcct gcccttcggt
     9961 caaaaaactg atgcgcgcat ctttttcggg caatacgctg aaattgcgcg gcaatattcc
    10021 cgaaagctgt ttgggcaagc ctgtcggtgt ccggatgttc gcgcttgacg aactgatccg
    10081 ccaaagtttt accaaccgct ggctgctcgg cggcggacgg atttcagacg gcatcggcat
    10141 agccgacaca ccggaaggcg cgcagacgct tgccgttgca cacgccaaac cgatgaagga
    10201 aattttgacg gacatgaaca agcgttcgga caatctgatt gcgcgttccg tcttcctcaa
    10261 actcggcggc gacggcaaac tgcccgccgt ttccgaacag gcggcgtctg ccgtccggcg
    10321 agaacttgcc gtatcgggca tcgatgttgc ggatttggtt ttggaaaacg gttcgggtct
    10381 gtccagaaaa gaaagggtaa cggcgagaat gatggcgcaa atgttggaaa cggcttattt
    10441 cagcccgttt gcacaagatt tcatcgacac gctgcccatc gccggcacag acgggacttt
    10501 acgcaaccgc ttcaaacaaa gcggcgggct gttgcgctta aaaaccggca cgctcaacaa
    10561 tgtccgcgcc cttgcaggtt attggctggg cgacaaaccg atggcggtgg tcgtcatcat
    10621 caacagcggc cgcgccgttt ccctgctgcc cgacttggac aacttcgttg ccaaaaacat
    10681 catctccggc ggcgacggct ggctggatgc gaaactgatg tgcaaagaac gccgcgcctg
    10741 aatcgggaaa atataaaaat gccgtctgaa ctgttcggac ggcatttttg attttcaaac
    10801 cggaattaca gccccgctgc cgctttcaat gaggccgctt tgtcggtgcg ctcccaagta
    10861 aactcaggtt cttcgcggcc gaaatgtccg taggcggcag attttccgta aatcgggcgc
    10921 aagaggtcga gcatttggac gatgcctttg ggacgcaggt cgaaatgttc gcaaaccagc
    10981 gcaatcagtt tttcctcgct gattttaccc gtaccgaaag tatcgatgga aatcgaagtc
    11041 ggttcggcaa cgccgatggc gtaggaaact tggatttggc attgggttgc caagcctgcg
    11101 gcgacgatgt ttttcgccac ataacggcag gcgtaagcgg cggaacggtc cactttggac
    11161 gggtctttgc cggagaatgc gccgccgccg tgcggagccg cgccgccgta ggtatcgaca
    11221 atgattttgc ggccggtcaa accgcagtcg ccttgcgggc cgccgatgac gaagcggccg
    11281 gtcgggttga tcaggtattt tgtttcgtcg gtcagcagtt cgggcggcaa aacgggcttg
    11341 ataatctgct caatcacggc tttactcagt tcttcatggc tgatggcagg atcgtgctgg
    11401 gtggacagga cgacggtatc aatgcgtttc accttgccgg tttcgctgtc gtaaaccacg
    11461 gtcagttggg cttttgcgtc agggcgcagc caaggcaggc ggccgtcttt gcgcaattcg
    11521 ctttgacgct gcatcaggcg gtggctgtaa tagatggcaa acggcatcag ggtcggggtt
    11581 tcgtcgcagg catagccgaa catcaagcct tggtcgcccg cgccttggtt caggtcgatg
    11641 ccttcgcctt cgttcacgcc ttgggcgatg tcgggggatt gttggtcgta gtacacgccg
    11701 accgcgcagc cgttggcgtc aaagcccagc tcggaggagt tgtagccgat gcgtttgatg
    11761 gtttcgcgtg cgactttgat gtagtccact tgggcggtgg tggtgatttc gcccgccaat
    11821 acgcacaagc ctgtgttgac caaagtttcc gccgcgacac gcgctttggg gtcttgcgcc
    11881 aagatggcat ccaaaatcgc atcggatact tggtcggcta ctttgtccgg atggccttcg
    11941 gaaaccgatt cggaagtaaa cagatattcg ctcattgaaa ttttccttga aaaacaaacc
    12001 atcttcttca gacagcatgt tgtatgaaca taatgtcgac agcgggaaat atagcaaaat
    12061 ttccctattc gtgccattca gttgagaaat attcctattt aaacggcgct ttggaatctc
    12121 tgtccgtacg tttcgtacag gcaaaaaaat tcccgcatca agcgggtttg gattgctccg
    12181 gcgagccaca tcggtttttc aaccgtccac cttacctttc cgtttgaaaa gcaggttggc
    12241 atggaattcc caactcttaa tgcagcgcgc atcgtagcag aaaaggcata ttgccgcaat
    12301 acttcccttt ttcagacggc atgtttcgtt tacaattcag gctgtttccc cctttgcgaa
    12361 ccgccatgca catcctgttg accgccctgc tcaaatgcct ctccctgctg tcgctttcct
    12421 gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta aaggaagacc
    12481 gcgcgcgcat cgtcgccaat atgcggcagg cgggtttgaa ccccgacacg cagacggtca
    12541 aagccgtttt tgcggaaacg gcaaaatgcg gtttggaact tgcccccgcg tttttcaaaa
    12601 aaccggaaga catcgaaaca atgttcaaag cggtacacgg ctgggaacac gtgcagcagg
    12661 ctttggacaa gggcgaaggg ctgctgttca tcacgccgca catcggcagc tacgatttgg
    12721 gcggacgcta catcagccag cagcttccgt tccacctgac cgccatgtac aagccgccga
    12781 aaatcaaagc gatagacaaa atcatgcagg cgggcagggt gcgcggcaaa ggcaaaaccg
    12841 cgcccaccgg catacaaggg gtcaaacaaa tcatcaaggc cctgcgcgcg ggcgaggcaa
    12901 ccatcatcct gcccgaccac gtcccttctc cgcaggaagg cggcggcgtg tgggcggatt
    12961 ttttcggcaa acctgcatac accatgacac tggcggcaaa attggcacac gtcaaaggcg
    13021 tgaaaaccct gtttttctgc tgcgaacgcc tgcccgacgg acaaggcttc gtgttgcaca
    13081 tccgccccgt ccaaggggaa ttgaacggca acaaagccca cgatgccgcc gtgttcaacc
    13141 gcaataccga atattggata cgccgttttc cgacgcagta tctgtttatg tacaaccgct
    13201 ataaaacgcc gtaacgaaaa taaaaatgcc gtctgaacag gtttcagacg gcatcttgtc
    13261 atttgacgat ttcggacaac gaccaacgcg gacggacgtt gaacgcaccg acctccctgc
    13321 ccttgcccag gtgcatcgca ccggcaaagg caatcatggc accgttgtcc gtgcagtatg
    13381 ccatcggcgg gaaaaacacg ctgacttttt cggacggatg tttcggcttg cctttggggg
    13441 tcgggatttg taccgtcatg ttgccgaaag tttcacggag tttgcggttt gcaccgaccc
    13501 cgccggcgac cactacggtt ctgaaccctg tctgcaacag ggcttttttc acttttgcct
    13561 ccaacacttc tactaccgca tcttgaaacg cacggcaaat attgttgcgg gtttgctcgg
    13621 gtatggtttc cgaaccgttt gcctcacgca ctttttcaac ggcagtcaaa acagcggttt
    13681 tcaatcccga aaaactcatc tgcaaatcgt cggaatgaat catcgggcgc ggaaaaacaa
    13741 acgcttcggg cctgcccgat tccgcaagtt ccgacagttt cgcaccgccc ggatacggca
    13801 aacccagcag tttcgccgtt ttgtcgaatg cctcgcccgc cgcatcatcg acgctctcgc
    13861 ccaaaagctc gtaatcgcct atgcccctga ccgccataat ctgcgtatgc ccgcccgaaa
    13921 ccaacagcgc gacaaaagga aagtcgggtt tttcctccgc caacagcggc gacagcagat
    13981 gtccttccaa atgatggacg gggatgacgg gcttatccaa cgctaaagcc agcgcgttgg
    14041 cgtagctcga acccgccagc agcgcaccgc ccaaaccggg cccctgcgta aaggcaaccg
    14101 cgtcaatatc gccatacgat gcgcctgcct gcgccaaaca gccttcagtc aacggaacaa
    14161 ggcggcggat atggtcgcgg ctcgccaatt ccggcacgac cccgccgtat tccgcgtgca
    14221 ttgccatttg agtgtgcagg cagtgcgacc gcaatcctcg ttcggtgtcg tacagcgcaa
    14281 cgccggtttc gtcgcaagaa gattcgattc ctaataccaa catggtctga tgccgttaaa
    14341 aactgaaaaa cgtattctag cgaatttcgg cacgactgcc gtatccccaa aacggaacat
    14401 gccgtctgaa gcccttcaga cggcatcgtc gcaccgtatc aaagcgttcc gtaagaatgc
    14461 agcccgctca aaaacatatt cacgccgata aaggcgaatg cggttacgaa caaaccgata
    14521 atcgcccacc acgccagcac tttgccgcgc cagcccgcca ccagccgcaa atgcagccac
    14581 acggcgtaat tcaaccaaac gatgaacgcc caagtctctt tcggatccca gctccaatag
    14641 cgtccccaag cgtccgccgc ccacaacgcg cccaaaatgg tggcaatggt aaagaacaaa
    14701 aagccgacgg caatcgcctt atacatcacc tcgtcgatca atgccgacgg cggcagccac
    14761 agttttccgc ctttttcttc cgcacgcagg gaaaccagtt cggcaatacc gagcatcgcg
    14821 gaaatgcaga acgcgccgta accgataaag tttgccggaa cgtggatttt catccaccag
    14881 gactggagcg cgggaatcag cggctggatg gtatgcgcct cacgcgacac gctgtaccac
    14941 aacacaaagc cgaccacgac cgccataaag ccgaacacga agccgcccaa tttctgcacg
    15001 gcaaatttac cctcgtaata caaatacatc agcgcggtaa tgaccaaaaa cagaatgaac
    15061 acttcataaa ggttggacac ggggatatgc cctgcatcgg gacggagcag atagctttcg
    15121 tgccaacgta ccagcagacc ggtaaagcct gctacggcag acacccatgc aaacacggtt
    15181 cccataccca acagcgtgtt ggtcggcaca tttttcacgc ttgccaaaac cgcgcccgaa
    15241 atataggcga acagggcgaa gaagacaaaa gcacactgcc acatgatcgc cgattggctg
    15301 ctgaggaaat accgcaacag gaaaatctct gccgatttaa tgttgccgcc atacagaccg
    15361 acggcggcat aggcaagcaa tacgcttaaa ggaacaaacc agcgcatcgg tttgaaaaac
    15421 caacccaaaa acacggcaat accggcactc gcccacaaca tgaccgtttc gtaaatgtcc
    15481 atatgcatac cggaacgggt ctgcacgaaa accgtagccg caaaaaccag cacggcaaat
    15541 acccaatccc aaagattcag attgcggatc aaagatttct gaatcagcag ctcgtgttcc
    15601 ggaagggttt tatagtgttc agtcatgatt caagtccttg ccgagccgtt gcaggctctc
    15661 gacgtgtttt ggaaattcct tctgcaaatc ccgttcgctg cgggccgaag acatagcaaa
    15721 acggattttg ccgtctgaaa acaataccca cgcccgtttt tcgcgcacat aaaacataaa
    15781 taccgtaccc aaaaccaaca ataccgagcc gagatagacc aaaagcgcac ccggcgaacg
    15841 ggtcatctgc aaacctgagg aacgcacctc ggaaaacccg tcaagctgga gcagcatagg
    15901 cgcgggatat tccgtcagcc ccgtataggc atccatactg tgcagcagga aacggttccg
    15961 cgcttcatcc tgccgccatt cgggcaagcc gtaccggcgt atggtttcat ccaaagcagc
    16021 gttcatcacg ccgtaaagca tttcgtagaa atagccctgc atcttatcct gctgcccttt
    16081 cgggatattg gacgtaataa attcgtccaa tcccaaatag cctttttgcg caaagatatt
    16141 cagcgtgttt tccgcagcca gcatgaattg ttcgcggatt tcggcaggtg cgtctttggt
    16201 tgcgtcggca accagacgtt tgcgcccttc cccatctttc aaaaactcac gcaatgccat
    16261 aaaggtgtcc gctttcaact gcttgtccaa ggggatacgc agccagcggt attgctgctg
    16321 caagccgctg cgcgtgccgg tcagccaaaa ataatctttg tcctgcaaaa tcggcagcat
    16381 atagttttta tattcgaccg cctgccctgc cgcatcacgg atgcggtaca cgatggaagg
    16441 gccgatattg gtgtattttt taccttcctg agtaacggcg cggacatcgt tcagagtgga
    16501 tttcaggctt ttttcccgtt ccgcaccctc gctcatgtcc tccacattca tagaagtgaa
    16561 ctgatcgaac tcaagacgat atttgtgttt gccgatttcc aacggaaact ggtgtatgga
    16621 ggttgccttc aacacgacag gttcgcgcga agcatccctc aaattccacg ccttgaatgt
    16681 caaatccgaa ccgccgtcgg caaaactcgc ctgataaatc gtgatgccgt gcaaggtcaa
    16741 aggatggttc acgcggatgg tgcgctcgag tttctcaccg gttgccttgt ccgttacttc
    16801 aatatcgctg gcaaaatcgc gcggcatacc cgtattgtaa aaatcgatat ggaatttttt
    16861 cagtttgact tcaaaaggca agtcctgaac caacatcccg ttgtcggcat tcaggaaaac
    16921 cacatccgca ctttgcccct cggaaatatt gacgttgccc ctaaatgaga gattggacgc
    16981 acccaaaata ctttcgggct tgaaatcctt ggcataaacc gcctgattgt ccggaacaat
    17041 ccgaccggcc agcataccca gcttcagcag caggttactg tctatcaacc cgcccaggca
    17101 aatgacaatc aaagcaacat gggcaaagat atagccccat ttgttcatcg tgcctttttt
    17161 ggcggcaatc agaaccgacc cgtcctcacg gctgacggtt tttccctgaa aaccccgcac
    17221 ctccagataa cgtttggcaa cttcgggggc aatttttaca tccaacagcg aagaatggcg
    17281 catcgccgcc agagattttt ctttaacctt ttcccggaaa gacttcattt cgcgccaaaa
    17341 cggcggaacg ttacggatta aacacaaact ggtagaaacc accagaaaca tcatgataac
    17401 gacaaaccat gccgaagcat agacatcgta caaacccaaa aaatcaaaaa tccgagtcca
    17461 aaacggtccg aatttgacca aataatccgt ctgcggctgg ttttgctgta acaccgtgcc
    17521 gataaccgat gcaataccca gcagactgag caaagcgacc gcaaagcgca tggagctgaa
    17581 aaaagcgaac cacggacggg aaagaagtgt gggggatata cgggatttac tcattgtgtg
    17641 tttattccgc catcaggaat atgggaaagc agaattgggc agacagaaaa caacgtcccg
    17701 attctactgt cttgatgctt tttatttcaa gacaatgaag acagcctgca tcgattccaa
    17761 cggttgcgat tgaaaaaact tatcgcaaaa ttttcagaag ccgtctgaaa accttttcag
    17821 acggcttctg aagcagaccc ttggatgatt aacgcaaacc ctggataaag ttagcaactg
    17881 cttttaaatc ttcttcggac atacggtttg caatatcttc catgatggta tttttacgct
    17941 gaccggactt gtaggcattc atctgttcaa caatatatgc ctggtgctgg ccgcccaaac
    18001 gcggataagc ctgaatttcg cttccgcctc ccggcatacc cgcaccgctc ggaccgtggc
    18061 aggacataca cgccggcact tttttatcgc tcaaaccgcc gcgatagatt ttcgcgccca
    18121 attcgggatt ttccttagga ttggcttcgc cggatttggg ctgctgtttg gcatagaatg
    18181 cggatacgtt caaaatatcc tgatcgctca aattcattac caccggtttc atcacagctg
    18241 ccgaaccgtg ggtgcgtttg ccgtcgcgga tgccgatagt ttgatgatag atgtaagcag
    18301 tatgctgtgc cgccaaacgc ggatacatcg caatgccgct gttaccgtct gctgcatggc
    18361 aagccgcaca aaccgttgcg gcaacctgtt tgcctttttc cacgtctgct ttgggagatg
    18421 cggaaaccgc accggcagcc aaaacaaagg ccaataaagt caatcgtctc atggagtgct
    18481 cctgattaca gcattggata acgcaacaat gctctttttt atattcaaat atgggatttt
    18541 tgacccgatt aaaaccgatg attctgtaaa cgtgttattc tatactaaat ttacattaaa
    18601 ttaccactgt gtttcacata aaaccaaccg catatttttg ctgtcggaca aacggcggcg
    18661 gaaaacaagg atatgcccat gaaccttttt caaaacgcca aattcttcac gacggtaaac
    18721 catctcaaag acctgccgga cacaccgctc gaaattgcct ttgtcggcag gagcaatgcc
    18781 ggaaaatcca gtgccatcaa taccctgacc aaccatgtcc gtcttgccta cgtttcaaaa
    18841 acaccgggac ggacacagca tatcaacttc ttcgagctgc agaacggcaa ttttatggtc
    18901 gatttgcccg gctacggtta tgcccaagtc cccgaagcgg tacgcgcaca ttgggtcaat
    18961 ctgctcggcg actacctccg ccatcggaaa cagcttatcg ggctggtttt gattatggat
    19021 gcccgccatc ctttaaaaga actcgacatc cgtatgctgg actttttcca cacgaccggc
    19081 agaccggttc acatcctgct gtcaaaagcc gacaaattat ccaaaaacga acagataaaa
    19141 accctgtccc aagtcaaaaa actgctcaaa ccttattccg acaggcaaaa catcagcgta
    19201 cagctgtttt ccagcctgaa aaaacaaggt attgacgagg ccaaccgaac tgtcggaagc
    19261 tggtttgacg cagcagatgc cgccgcttcc tctccagagg aaaactgacc tcaattatac
    19321 ggaaaccgta ttccccccac ttgaccggcc gcaaacattt aaaaaattgc cactgccaaa
    19381 tctaaaatgc cgtctgaaag acttttcaga cggcattttg cggagtcttt aaaacaggga
    19441 atccaactgc tgctgtttgg aatccgtatt gctcggaagc accggcgttt cctgtacgtc
    19501 ctgacgcgtt tcgtcagacc ttccctgctg ttcgttttct actgccgctt catcatcttc
    19561 ttttgcccgt cgggaaggtt gcggcgcaat accgctgttg tccagcatca agcccggatc
    19621 ggttaccata cgttccttca tatagtattc gccattgctg ctgaccacac cttcaggcat
    19681 tttcatccct ttgccctgct ttcctttcaa cgcaaaacgc atatagtcca cccaaaccgg
    19741 caccgcaatc gtaccgccgt agccggcacg ccccatactc ttaggtttgt cgaagccgat
    19801 atatacggca gtaaccacat cagggttaaa accgacaaac cacgcatctt tattgtcgtt
    19861 ggtcgtaccc gttttaccgg caatatccgt tcttcccaac gcagctgccc cccttgccgt
    19921 accgacacgg accacatcct gcataatctt atacataata taggcattgc gcggatcgat
    19981 tgcctgaggc gcattttgcc ctgccaccaa aggttgcatt tgggcgcgca acctgccgtc
    20041 tctgtcataa atcttatcga tcacgtgcga agaaacccta tatccgccgt tcgcaaatac
    20101 actatatgcc tccgccactt tcaacggcgt cgtctcgccc gtacctaaag ccatagacag
    20161 gcttgccggc agctcggacg gcctgaagcc gaaacgccgg atatactgtt gcgcgtaacc
    20221 gacaccgata gacatcaaaa tacggatgga aaccatattc ttggaagccg tcagagcctg
    20281 tctcaaagta atgtagccgg aatatctgcc gtctgaattt ttaggtgtcc aaaccgaacc
    20341 gttcggccct ttccccggca gggaaatcgg cgcatcgtta accactgtgg acgcggtcat
    20401 ccccttagat aatgccgccg aatagacaaa cggcttaaag gtcgaacccg gctgccgcat
    20461 tgcctgaacg gcacgattga atgttttgct gtgaaaatca taaccgccga ccagcgcgcg
    20521 cacagctccg gtttttgcat ccagcgaaac caaagccccc tgcagcaacg gctcttgaac
    20581 caccgcccaa cgcccgccgt tgtttttgac acggatgacc gcgcccctgc ggatacggtc
    20641 ctcccccatt ttctcattat cgaccgctcg ggccgcaaaa cccaaggcgc gcctgtcaag
    20701 cgcaacccgc ctgccgccgg gcagctgtat gacgacattt ttctttttag taacatccaa
    20761 cacaacggcg ggaaccattt tatcgacggt atagagtccc gacagatact ggctgacagt
    20821 ctcctcgaca tcttcactct tactcaaatc gatatagttt tccgcaccgc ggtagctgct
    20881 gccgcgatcg aaattccgta gagccttgcg caatgcctcg gttgccgcct tctgatgatc
    20941 ggtgcggacc gtggtataaa ccttaaaacc ctgcgtatag gcatcttcac catatttctc
    21001 atacagttcc cgacgcacca tttccgccac atataaagca ctctgatcga ttttccgaac
    21061 aaaccgctca taatgcagtt cctcattcaa tgcctgatcg cgctgttgca cggtaatcat
    21121 cttctcctcg agcatattgt tcaaaatata cttctggcgc aacttggcac gctccggatt
    21181 aacaatcgga ttataggcag acggagcctt gggcagtccc gcaagcatgg cggcttccgc
    21241 caaagtcaaa tctcggacat tcttattgaa atagatttgc gcggcagatg caaaaccata
    21301 ggcgcgctga ccgaggtaaa tctgattgaa atacaactca aggattttgt ctttgcttaa
    21361 agactgctcg attttatagg caagcaacac ctcattgaat ttgcgtgtga acgttttttc
    21421 actgctcaaa taaaaatttt tcgccacctg ctgtgtaatc gtactcgcac ccgactgcac
    21481 gctgccggac acgacattgc cgacggcagc gcgggcaaca ccccaaacat ccacccccca
    21541 atgccggtaa aagcgtttat cctcggcggc aataaccgca ttccgcaaca cctcggggaa
    21601 atcgccgatt tttgtaaatt cgcgccgctg ctccccatac ataccgatga cttctccatc
    21661 cgccgaataa atagtcaacg gcattttagg ctggtaatgc tgcaaagaat ccaaagacgg
    21721 cagtttcgga tacgttacca aaatagcaat ggcaaccaga cccactccaa atacacaaaa
    21781 accaaaaaac aaaccaaaac aagtagttaa aatcttttta atcatagctg aataataatt
    21841 taccattatt ggtattaaat aaagtaaaat agcaaccgat ttctacaaag cacggtttca
    21901 atgtgcaaag aacaaggaat ccattacgga taccgaaacg gttactcact gtacaaataa
    21961 agcaggaact ttcatcatgc gcttgtttaa aagcttgaaa aaccctaaaa aaacagatgc
    22021 caagctccct aaaaaatctt cgggactcaa taaccgcgcg gcaatcggca tcgatatcga
    22081 ccagcattcc atcaaaatgg tacaattgtc aggacgtagt ttaaaccaaa ttcaattgga
    22141 aaaatacgtc attgccaaat taccaaagaa tatcattcaa ggcaataaag tccaaaatta
    22201 cgatcaactt gttacatatt tgcaacaagc ctatgccaaa ctgggtactt cgtgcaaaaa
    22261 catcgtcgcg tccgtcccgc aaaatctggc aaccatcgaa caattgacct acacggccaa
    22321 agatgcagaa ttggacctgc aggggttcgt ggagtcctcc atctccgaag ccagctcgat
    22381 atcgctcgaa gaagccaatt acgactatca ggtcttgtcc caatcggccg tcggcgaagc
    22441 tgttttgtcc gtcgcatcga gaaaggatga aatcgaaccc ctgattgacg cattcaacgc
    22501 agccggtatg aaattatccg cgcttgatgt ggacattttc ggacaataca acgcctacgc
    22561 gctgtggata aaccattttg cccccgagct tgcggacgaa aaagtggcca ttttcggcgt
    22621 atatgccgca cagacctacg ccctggtcat ccaagacgga aaaatcctat acaaacagga
    22681 aacctccgtc agcgcagaac agctcaacca actcatccag cgcacctatc aggtaacagc
    22741 agaaaaagcg gaagagatca tcaactcccc gcaaaaacct tccgattacc aagaaagcgt
    22801 ggcaaactat ttcaaccagc agattaccca agaaatacaa agggtcttgc agttttatta
    22861 caccacgcag accgcagacg atatgaccga catcaagcat atcctgctga cgggggaagc
    22921 ggtgcgccag aaaggcatcg cccaaaccgt cgcctcgcaa accaatgcgg atgtacaatg
    22981 cgtccatccc gcgcgttatt ttgcgaacga cctcaaaaca gacgaacaac aattcgaact
    23041 tgatgcgccg acactgacca aggcgttcgg tttggcggta cggggattat aattatgaac
    23101 aatttaatca aaatcaacct cctcccctac agggaagaga tgaacaaacg caaacagcag
    23161 cagtttaaaa cgctgatgta cggcgccgtg ctgacgggcg ttgccgccgt tgcggcaacc
    23221 tacctgttta tcgacaatat gatcaataaa cagtcggaaa aaaacacgct gctggaaatc
    23281 tccatcgcac acttggatac cgagctgtcg gaaatacaaa agctcaaaca ggaaaaagat
    23341 gccttcctga ttaagaaaaa caaaatcggg gagctccaga tcaaacgcct ccaagccgca
    23401 aaaatcctcg acagcctgaa tgaggccgtc cccggaagca cctacctaac ctcgctggat
    23461 gccgttaccg ccgactctta tcggctcagc ggcaggacat ccagcgacaa ccgcgttgcc
    23521 gccatgatga gggcgatgcc caataccggc atattcaagc aacccgaatt gttaagcatc
    23581 aagaaaaaca attcgcatca agaatttacc cttcaggcaa cattacaacc tatcgtaaag
    23641 gcggccgaat ccaaagagaa tccggcttcg ggaaacgcac aggaggcaaa ctgaatggct
    23701 tctaaatcat ctaaaaccaa cttggatctc aacaaccttc acctgctcaa ccttcctgcc
    23761 aggcttttta tcgccctgct ggtcgttgcc gccgtgctgg ggctcggtta tgccggattg
    23821 ttcaaaagcc agatggaatc ccttgaggaa tatgaagcaa aagaaaccga actgaaaaac
    23881 acctacaaac agaaaagtat cgacgcggcc agcctgaaca accttaggga cgaacttgcc
    23941 tcaatccgct ctgccttcga tatcatgttg aaacagctgc cgacagatgc agaaattccc
    24001 aatttggttc aagagcttca tcaggcgggt tcgagcaacg gtctgcgctt ggacagcgtt
    24061 atgccccaac ctcccgtaga tgacggtccc atcaaaaaat taccctattc catttccatt
    24121 accggaaatt acgaacagat cagccaattt acccgcgatg tcggcagtct ctcccgaatc
    24181 attacccttg agtcgctgaa aattgcccaa tctccggaaa acggcggcaa tcctgacggc
    24241 aagagcagta tcctgaacct cagcgccatt gccaccacct accaagcaaa atccgtagaa
    24301 gagcttgccg cagaagcggc acaaaatgcc gagcaaaaat aacttacgtt agggaaacca
    24361 tgaaacacta tgccttactc atcagctttc tggctctctc cgcgtgttcc caaagttctg
    24421 aagacctaaa cgaatggatg gcacaaacgc gacgcgaagc caaagcagaa atcatacctt
    24481 tccaagcacc taccctgccg gttgcgccgg tatacagccc gccgcagctt acagggccga
    24541 acgcattcga cttccgccgc atggaaaccg ccaaaaaagg ggaaaatgcc cccgacacca
    24601 agcgtattaa agaaacgctg gaaaaattca gtttggaaaa tatgcgttat gtcggcattt
    24661 tgaagtccgg acagaaagtc tccggcttca tcgaggctga aggttatgtc tacactgtcg
    24721 gtgtcggcaa ctatttggga caaaactacg gtagaatcga aagcattacc gacgacagca
    24781 tcatcctgaa cgagctgata gaagacagca cgggcaactg ggtttcccgt aaagcagaac
    24841 tgctgttgaa ttcttccgac aaaaacaccg aacaagcggc agcacctgtc gcagaacaaa
    24901 attaagaaga ggattactcc attatgaata ccaaactgac aaaaatcatt tccggtctct
    24961 ttgtcgcaac cgccgccttt cagacggcat cggcaggaaa cattacagac atcaaagttt
    25021 cctccctgcc caacaaacag aaaatcgtca aagtcagctt tgacaaagag attgtcaacc
    25081 cgaccggctt cgtaacctcc tcaccggccc gcatcgcctt ggactttgaa caaaccggca
    25141 tttccatgga tcaacaggta ctcgaatatg ccgatcctct gttgagcaaa atcagtgccg
    25201 cacaaaacag cagccgtgcg cgtctggttc tgaatttgaa caaaccgggc caatacaata
    25261 ccgaagtacg cgggaacaaa gtttggatat tcattaacga atcggacgat accgtgtccg
    25321 cccccgcccg cccagccgta aaagccgcgc ctgccgcacc ggcaaaacaa caggctgccg
    25381 caccgtttac cgagtccgta gtatccgtat ccgcaccgtt cagcccggca aaacaacagg
    25441 cagcggcatc ggcaaaacaa caggcggcga caccggcaaa acaaaccaat atcgatttcc
    25501 gcaaagacgg caaaaatgcc ggcattatcg aattggcggc attgggcttt gccgggcagc
    25561 ccgacatcag ccaacagcac gaccacatca tcgttacgct gaaaaaccat accctgccga
    25621 ccgcgctcca acgcagtttg gatgtggcag acttcaaaac accggttcaa aaggttacgc
    25681 tgaaacgcct caataacgac acccagctga ttatcacaac aaccggcaac tgggaactcg
    25741 tcaacaaatc cgccgcgccc ggatacttta ccttccaagt cctgccgaaa aaacaaaacc
    25801 tcgagtcagg cggcgtgaac aatgcgccca aaaccttcac aggccggaaa atctcccttg
    25861 acttccaaga tgtcgaaatc cgcaccatcc tgcagatttt ggcaaaagaa tccgggatga
    25921 acattgttgc cagcgactcc gtcaacggca aaatgaccct ctccctcaaa gatgtgcctt
    25981 gggatcaggc tttggatttg gttatgcagg cgcgcaacct cgatatgcgc cagcaaggga
    26041 acatcgtcaa catcgcgccc cgcgacgagc tgcttgccaa agacaaagcc ttcttacaag
    26101 cggaaaaaga cattgccgat ctgggcgcgc tgtattccca aaacttccaa ttgaaataca
    26161 aaaatgtgga agaattccgc agcatcctgc gtttggacaa tgccgacacg accggaaacc
    26221 gcaacacgct tgtcagcggc aggggcagcg tgctgatcga tcccgccacc aacaccctga
    26281 ttgttaccga tacccgcagc gtcatcgaaa aattccgcaa actgattgac gaattggacg
    26341 tacccgcgca acaagtgatg attgaggcgc gtatcgtcga agcggcagac ggcttctcgc
    26401 gcgatttggg cgttaagttc ggcgcgacag gcaggaaaaa actgaaaaat gagacgagcg
    26461 cattcggctg gggcgtgaac tccggcttcg ggggcggcaa taaatgggag gcccaaacca
    26521 aaatcaacct gccggttgcc gctgccgcaa acagcatttc gctggtgcgc gcgatttcct
    26581 ccggcgcgtt gaatttggaa ttgtccgcat ccgagtcgct ttcaaaaacc aaaacgcttg
    26641 ccaatccgcg cgtgctgacc caaaaccgca aagaggccaa aatcgaatcc ggttacgaaa
    26701 ttccttttac cgtaactaca gcctcgggcg gcggcaactc taccaacacg gaactcaaaa
    26761 aagccgtctt ggggctgacc gttacgccga acatcacgcc cgacggacaa atcatcatga
    26821 ccgtcaaaat caacaaagac tcgcctgcac aatgtgcttc aggcagcaac acaatcctat
    26881 gtatttcgac caaaagcctg aatacgcagg ctatggttga aaacggcggc actttgattg
    26941 tcggcggtat ttatgaagaa aacaacggca atacgctgac caaagtcccc ctgttgggcg
    27001 acatccccgt tatcggcaac ctctttaaaa cacgcgggaa aaaaaccgac cgccgcgaac
    27061 tgctgatttt cattaccccg aggattatag atacggccgg caacagcctg cgctattgat
    27121 gcgtcaaaat aagggcatat gttttacagc atatgccctt tctttatgct ttttgccgcg
    27181 accgaaatgc cgtcattccc gcgcaggcgg gaatccaact tgtccggttt cggttgtttt
    27241 tcgtctcgta acttttgagc cgtcattccc gcgaaatcgg aaatccagtc cgttcagttt
    27301 cggtcatttc cgataaattc ctgttgcttt tcatttctag attcccactt tcgtgggaat
    27361 aacggcggaa gggataaatc ctcgcaatcc aaagcctcgt catttccaca aaaaacagca
    27421 acccgaaaca ccccgtcatt cccgagcagg cgggaatcta ggtctttaaa cttcggtttt
    27481 tttccgataa attcttgcct cattgaaatt ctagattccc gctttcgcgg gaatgacggg
    27541 atatgggttt ccgtgcggac gtgttcggat ttccgcctgc gcgggaatga cgattcgggt
    27601 attactgacg attcatcggg ccgtagggtg ggcttcagcc cactaatttc accgcatcaa
    27661 gggttttggg gaataggcgt aactcggcgg tttcggtttt tggcgaagtt ggttggaatc
    27721 ggcgggctga agcacaccct acagtccacc ctacaaccca tcttacgcat ccgccttacg
    27781 catttatcct tcgcctgccg atatgccgtc attcccgcac aggcgggaat ctagaaccgc
    27841 aacgcggcag gaatct
//
LOCUS       DAWTCI010000018        27337 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWTCI010000018 DAWTCI010000000
VERSION     DAWTCI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27337
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.018"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(49..720)
                     /locus_tag="WM481_000792"
                     /pseudo
     CDS             complement(49..720)
                     /locus_tag="WM481_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            787..1089
                     /locus_tag="WM481_000793"
     CDS             787..1089
                     /locus_tag="WM481_000793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080154.1"
                     /translation="MKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAGFNGDI
                     GEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWEMER"
     gene            complement(1171..3258)
                     /locus_tag="WM481_000794"
     CDS             complement(1171..3258)
                     /locus_tag="WM481_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9080155.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHIIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAIFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(3261..4526)
                     /locus_tag="WM481_000795"
     CDS             complement(3261..4526)
                     /locus_tag="WM481_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFA9080156.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKREQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(5247..5528)
                     /locus_tag="WM481_000796"
     CDS             complement(5247..5528)
                     /locus_tag="WM481_000796"
                     /inference="COORDINATES: protein motif:HMM:NF021923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFA9080157.1"
                     /translation="MSNDENLLFSQPHLTKVNIMQVLTLDEIQQVSGAACNWRDFSKN
                     TIGSALGGAAGGAIVGSFAGGIGAIPGAKFGAIGGAITGSVQYGSTCWW"
     gene            complement(5749..5985)
                     /locus_tag="WM481_000797"
     CDS             complement(5749..5985)
                     /locus_tag="WM481_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HFA9080158.1"
                     /translation="MKELHTSELVEVSGGKFHISAQGGGNLGKKDMVAVGKIGVSYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGFSKKW"
     gene            complement(6941..7267)
                     /locus_tag="WM481_000798"
                     /pseudo
     CDS             complement(6941..7267)
                     /locus_tag="WM481_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7527..8999
                     /gene="pyk"
                     /locus_tag="WM481_000799"
     CDS             7527..8999
                     /gene="pyk"
                     /locus_tag="WM481_000799"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFA9080159.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HILKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQTEYPEAVSTNLAIAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            9158..9757
                     /locus_tag="WM481_000800"
     CDS             9158..9757
                     /locus_tag="WM481_000800"
                     /inference="COORDINATES: protein motif:HMM:NF015316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HFA9080160.1"
                     /translation="MPFLFVHRPPFYGNRNDPFHTEKTVLLLGTAFAAASVHASGYHF
                     GTQSVNAQSTANAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGL
                     PVQGSKNGKITKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKL
                     GLTSIAVEPVAAWKLNERHSFGAGIIAQHNSAELRKYAD"
     gene            9779..10585
                     /locus_tag="WM481_000801"
     CDS             9779..10585
                     /locus_tag="WM481_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HFA9080161.1"
                     /translation="MLQATPSNPTAAAQIKADGHADVKGSDWGVGYQLAWMWDINDRA
                     RVGVNYRSKVSHTLKGDAEWAADGAAAKQQWNDNMLTPLGYTANEKARVKIVTPESLS
                     VHGMYKVSDKADLFGDVTWTRHSRFNKAELFFEKEKNIANGKKSDRTTITPNWRNTYK
                     VGLGGSYQISEPLQLRVGIAFDKPPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHVV
                     DAAYTHIHINDTSYRTAKASGNDVDSKGASCARFKNHADIIDLQYTYKFK"
     gene            complement(10668..10961)
                     /locus_tag="WM481_000802"
     CDS             complement(10668..10961)
                     /locus_tag="WM481_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080162.1"
                     /translation="MKANDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSIHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLYIFSRIPR"
     gene            complement(11273..11422)
                     /locus_tag="WM481_000803"
     CDS             complement(11273..11422)
                     /locus_tag="WM481_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080163.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVSYADVSTHRTAHKAS
                     PYVPS"
     gene            complement(11440..11637)
                     /locus_tag="WM481_000804"
     CDS             complement(11440..11637)
                     /locus_tag="WM481_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080164.1"
                     /translation="MNTVPKSRIPVKPLPEKTTAEAKVEKWRQLGADHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            complement(11647..12663)
                     /locus_tag="WM481_000805"
     CDS             complement(11647..12663)
                     /locus_tag="WM481_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9080165.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQDIRGSIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSPLLYGETSLADIVQQKTGGNVEFKDGVLTAAVRFLPAKDARTAFIDNTV
                     GMATQTLSAALLPYGVKSIVMIDGKAVTKEDAVRVLSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDVERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            complement(12762..13976)
                     /gene="gltS"
                     /locus_tag="WM481_000806"
     CDS             complement(12762..13976)
                     /gene="gltS"
                     /locus_tag="WM481_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFA9080166.1"
                     /translation="MEWAFNSYYTLIAATLVLLVGKVLVKKIKILRDFNIPEPVAGGL
                     IAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENTKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGSVTVILAVQTAVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            complement(14084..14188)
                     /locus_tag="WM481_000807"
                     /pseudo
     CDS             complement(14084..14188)
                     /locus_tag="WM481_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14229..14510)
                     /locus_tag="WM481_000808"
     CDS             complement(14229..14510)
                     /locus_tag="WM481_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080167.1"
                     /translation="MKQIKASDFTDEHFGLPTILDILSELEKPQPRTDSRPQTTTQRA
                     CPNQFGDGGCVCEVEAVSPTAGMYSDRCSGGFCPRFFCYFKYVYSHDAV"
     gene            complement(14529..15653)
                     /locus_tag="WM481_000809"
     CDS             complement(14529..15653)
                     /locus_tag="WM481_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9080168.1"
                     /translation="MSNNQPNSQIYEDYWAFTNAFTNYNDQKFCTALNICLDFIDANQ
                     DQPYSNELYEALQQNIAQDSVMASQRTSKAMNLASVRKAINQFVKMGFIEPFLSSYTP
                     LSRDYVQARTNRKRQTLLSKIVYTHSGFQRSVTENSNIRQINFLIKTLVEHPQGKLNK
                     KEIAAMMLVDLKTFQQDYLTETELNDYFQQGIESGFIERKYNQISYLWNLLDKLDDLK
                     RVGDDLYFAEDAQRIFGNLDEITVRKRDPYLHRLYKNQLQEESEEHYGNVKCMLEKLA
                     YPVLIASHIKPFILSDDTEAYDPNNGLLLSRTLDSLFDLKYISFDDEGNMVKSKRLSD
                     DVWRRWCDVKLDNNLLNDKRKSYLAYHRELMLQEDQEFHI"
     gene            complement(15716..16723)
                     /locus_tag="WM481_000810"
     CDS             complement(15716..16723)
                     /locus_tag="WM481_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFA9080169.1"
                     /translation="MNKKYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYSEK
                     FYFHDECQQLDLYRKNIGKLNNQYKRALAFTLMRRAMIRKMPYSRFTIPWEKVKQLRD
                     EEYSYAKYGRRRAYHNESFQSHFLQNLDDYNQAVFNNGWRHHAFNQDIFDLLPNIQAD
                     AVYLDPPYTGTMNNYFGFYGLLDSYITSSIPKPFANHFMDKKQAVELFKRVINHLKPF
                     KYWLLSYNNASYPNRDELEEMLGKNGRNVQILETPHVYKVTGKEKKQSHKEILFLVEN
                     T"
     gene            complement(16716..17717)
                     /gene="dcm"
                     /locus_tag="WM481_000811"
     CDS             complement(16716..17717)
                     /gene="dcm"
                     /locus_tag="WM481_000811"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9080170.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPRHQLLQKDLTTLTEQDLTNCLNGQSVDLVIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(17748..20021)
                     /locus_tag="WM481_000812"
     CDS             complement(17748..20021)
                     /locus_tag="WM481_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HFA9080171.1"
                     /translation="MNITRILSQELSATTVQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRQLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQPQDVEAAAQGYLNENIPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHTQVVEGKETEGEKFSDYFDRREPVRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPESSLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDANTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGAFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRD
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPTNSAMADAFAKLKR"
     gene            20136..21152
                     /gene="galE"
                     /locus_tag="WM481_000813"
     CDS             20136..21152
                     /gene="galE"
                     /locus_tag="WM481_000813"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFA9080172.1"
                     /translation="MTVLITGGTGFIGSHTAVSLVQSGYDAVILDNLCNSSAAVLPRL
                     RQITGRNIPFYQGDIRDCQILRQIFSEHEIESVIHFAGLKAVGESVAEPTKYYGNNVY
                     GSLVLAEEMARAGVLKIVFSSSATVYGDAEKVPYTEDMRPGDTANPYGASKAMVERML
                     TDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGVPNNLLPYICQVASGRLPQLSVF
                     GGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAF
                     EAASGLHIPYRIQPRRAGDLACSYADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPG
                     RYGD"
     gene            21153..22193
                     /gene="rfbB"
                     /locus_tag="WM481_000814"
     CDS             21153..22193
                     /gene="rfbB"
                     /locus_tag="WM481_000814"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFA9080173.1"
                     /translation="MQTAGKKNILVTGGAGFIGSAVVRHIIQNTRDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQYRPDAVMHLAAESHVDRAIGSAGEF
                     IRTNIVGTFDLLEAARAYWQQMPSEKREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKAAADHLVRAWQRTYRLPSIVSNCSNNYGPRQFPEKLIPLMILNALSGKP
                     LPVYGDGAQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKTNLEVIKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDAAKIRRDLGWLPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            22298..23164
                     /gene="rfbA"
                     /locus_tag="WM481_000815"
     CDS             22298..23164
                     /gene="rfbA"
                     /locus_tag="WM481_000815"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFA9080174.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNAAFQRLLGDGSDFGIRLQYAVQPSPDGLAQAFIIGEEFIGNGNV
                     CLILGDNIFYGQSFTQTLKQAAAKTHGATVFGYRVKDPERFGVVEFDENFNALSIEEK
                     PQQPKSDWAVTGLYFHDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTQESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLTKKDVETRAKPLEKT
                     AYGQYLLRLIGK"
     gene            23303..23860
                     /gene="rfbC"
                     /locus_tag="WM481_000816"
     CDS             23303..23860
                     /gene="rfbC"
                     /locus_tag="WM481_000816"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFA9080175.1"
                     /translation="MDIIDTALPDVKLLKPQVFTDGRGFFMETFRDGWFKENIADRTF
                     VQENHSNSSKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNRYQLWIPEGFAHGFCVLGDAAEVVYKCTDYYNPETEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(23904..25922)
                     /locus_tag="WM481_000817"
     CDS             complement(23904..25922)
                     /locus_tag="WM481_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HFA9080176.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDDRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVAVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWAYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPFALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(26117..27238)
                     /gene="dnaJ"
                     /locus_tag="WM481_000818"
     CDS             complement(26117..27238)
                     /gene="dnaJ"
                     /locus_tag="WM481_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFA9080177.1"
                     /translation="MSNQDFYATLGVARAATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGAS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCRGAGKHIKEPCVKCRGVGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         7278 a         6848 c         6154 g         7057 t
ORIGIN      
        1 ccgaccacgc aagccgccga acaaaaaata aggggctgtc ctagataact aggataaact
       61 cgattttact aattgtttta aaatggaaca agaactttta tctcactgtt gttaaaacgc
      121 cattcgcact cctttaaata cagctcaaaa tgcgctttgg gaatgccgtt aaacttgcgt
      181 aaatgacgtt ttgcctgatt ccaaaagttc tcaattccat taatatggtt ttgtcgttcg
      241 gcaaaatgtg tgctgtgatt gatacgaaaa cgaagtttca gcgaagctaa aatggctaaa
      301 ttcgctcaca tctaatacat catagctacg ataacaatcc gtataaacaa tactgtcagg
      361 tttcacttgt tcacggataa taggaaataa agtagcggtt tgagtattcg gtactgtaac
      421 cgtataaacc ttaccatttc gcttcaaaag accgaatacg gcgactttac cggcagcacc
      481 gcgaccgcgt ttgcctttgc gttgtccgcc aaaataactt tcatctgctt ctacttcgcc
      541 atcaaacatt tccaaatgcg gactgttttg ataaataagt aatcgtaaac gatgaaaata
      601 ataggctgcg gtatttttat taacgcctac taactctgct gccgttcttg cagttacacc
      661 tgcgacaaac agttcaatga gtttattttg tttatactgg cttagacgac tttttctcat
      721 agggataatt ctaacttaat ttgaatttcc ctagttatct aggacagccc cttaaaaaat
      781 atccctttga aagattttga tgaagtttgt gaaaagtggg agaaatcctt taaagaaaat
      841 ggaaaagaaa ttgaatataa tcaaaaatat tcaagaattt atggtgaagt tcgtggagct
      901 ggctttaatg gcgatatcgg agaaaagcta catcgttgtg ccgtgatgga atgggctgaa
      961 aatgagaccg tgaaattagc ccaaaaatgg gaacaagagc agaaaaaaca acaaattcaa
     1021 cagaaaaagg aaactgaaaa atcgccaaaa cacaaagcca gtcgtgatga ttgggaaatg
     1081 gaacgttaaa ccgctatttt catgtattct attaaattat ttgaaaaaag acttcacttt
     1141 ttgttaaaga aaaatgaagt cttgttctgt tcaacctaaa ttcagaattt tatctgccga
     1201 ttgaattgtt tcttggcggt gtgcaaccat tattttcata ataccaagac tttttaggtt
     1261 atggttaatt ttttgttcat tttctacatc taaatggcta cttgcttcgt ctaaaaatag
     1321 aattttgggt cgtttataca atgcacgagc caagataact ctctgctttt gtccacctga
     1381 taagatattt cccatatcgc caatcaaggt ctcatagccc attggcattt taagtatatc
     1441 gtcatgtatt tgtgccattt ttgcacattg ttcaatgagc tccatatttg ggctttcatc
     1501 aaaaaatgaa atattttccc caatagaacc tgcaaaaagg acatcatctt ggcgaacaat
     1561 cccgctcaat cccctaataa aggatggaga aacttgatat atatcatgcc cattaatact
     1621 aactgtacca gtttcaggtt ttaggctacc tgttaaaatg tttaacaaag tggacttccc
     1681 ccgaccagat tgtcctgtta aaacaactgc ttcattatct ttgaattcca aattaatgtt
     1741 ttcaaaaaga tatggctcat tatcagcata tctgaatgag acgttttcaa ctttaagaac
     1801 cagttgttca ttatctaatt taggtatatg attatactta ataatttcag tttctgtttc
     1861 atttaaagta atgtcagcca aacgttcagc atgaagccct aacattttga tttggatgta
     1921 ttggtcaacg agagaagctg ttctgctttc aaattgccct ttataagcca aaaaagccat
     1981 cagaacaccg actgtaaatg aaccatctaa aattgcgctt gcaccaagat aaattatgat
     2041 aatattttcc atgctaaaca acagtttatt tgaaaattca aataaagcag agagtttatc
     2101 tgttgtcagc ttggtattga ctgtattcac aaataggctc atccaagtgc catgtctttg
     2161 ataatgttta tcaaataatt taactgattg gataccacga atggtttcca tgaaatatga
     2221 gttttgtttg gcttcatgaa caatattttc ttctgttgca tttcttaatg ggtaatatgc
     2281 aagccaacga attagtatgt acaaaacaag tgttaaaaga acaatcagcg ataattgagt
     2341 gctgtaaatt gtcattaaca cgaaagtaaa aatagccatt aagctattta aaactaaaac
     2401 aaaaaaagta gaagttagtg tttcttggat atgatctatt gaaccaaatc ttgaaatcac
     2461 atctcctaaa tgtcgtttac tgaaatagtc attaggtaag tcaagtaacc ttttaaatat
     2521 attggctttc cattgtaaat taagagttgt agataggtgc atacctaccc atgcttgtaa
     2581 caggctaatt aactgttgca ggatagccag taaaccaaat cccaaagtaa gggtcagtaa
     2641 taaattttta tcagcagtta caatgatatg gtctattacc cattgcataa agaatggact
     2701 aaccaatgca aagacttcca aagaaatagc taatataagc atttgaatta aagagcgttt
     2761 taagcctgaa atccccctta atagagataa tattttgatt ttctttgttt cttttttctc
     2821 ttcaaaatgg gtattgggga ataattctag ggcaatccct gtgaattttt gtgaaacttc
     2881 gtccattttg atttttcgca taccgacagc agggtccata atgacgatac tgtctttgga
     2941 aatggaacaa agtacaacaa aatggtttaa gttccaatgg agaatgcagg gtagttgtaa
     3001 atttgacagc tcatctaact ctaaacgcaa agctcgtggc gttaaattca tttcattgcc
     3061 aaatctcatg atgtctgcaa gatttgcgcc ctttaatgac agggtgtatt tttggcgtaa
     3121 cgttcttaaa tcagtgtgaa agccataata ggacaagatg gatgtcaggc atgctaaacc
     3181 acattcagca acttctgttt gcagaatgac aggtagcttt ttgttaaatc caaaggacag
     3241 tcttgataaa taatccattt ttaattgatt tttcctgaaa tgctgtaaag tgggtcaagt
     3301 acccattcgt acaatttttt tcgttcatgg agaatatctg cttctaaaat catgccaatt
     3361 tgaagcggct tgttttctcc gtatgcttta atcgtttgtt tttccaattt aactttcaca
     3421 agataggcag gttcatttaa taaggttggg ttagtgaaaa tgatacctaa acctgatagc
     3481 tcttgtttac cgagagcagt tctggcaact gaaataattt ctcctgtggc atgtccaaat
     3541 ttttggtaag ggtacgcttg gtaacgtaaa acaactttat ctttcggttt aataaaacca
     3601 acagctttac tgggtatgta aagattggcg accaattcag tttgttcagg gacaatgctt
     3661 aacagcaatt tagacggttc aacttgttgc cctatatcaa cattaattgt tgaaatataa
     3721 cctgatttac tagctcgtat ggtttgttcg gatttcaaat caaaatccaa aatttcttgg
     3781 ttcatttccg taatcgcacg gttgagttgg ctcaattcgg ttttatggcg ttcaggcagg
     3841 ctgcttaatg tgattttctg ttcatcaagt tccctgattg cattattttg ttcacgtttt
     3901 aggctctcca aacgtgagcg ttgttccaat aaatggcttt cggcggtcat cttatcttgt
     3961 tgggatactg cgccttgact ggctaaaaac ttgttcttgt taagggtttt ttccgctaaa
     4021 cgaatttgac gattttgccc tgtaatttgc tgtttaatat tctctaattg attgtttaaa
     4081 cgatgaatgt tgttatgaac atttttttgc tcattttgat gaatgcgctt taaacgttcc
     4141 aattcttgta atgccaaagt ttttttaagg ttggcttctg ctgccaattt ggcttgtacg
     4201 tttccttttt cgccaaaacg cgatgtggaa agtttgaaca atggttcgcc agctttgaca
     4261 aagttaccat cttcaacaaa tttatgcgta atcgtgccga tatcggaaga gtaaacacga
     4321 accaccccca tagttggaag taattgacct tcaacggttg ttttattggt atagctacca
     4381 aaaatcaaaa agataatgat acacagagca atgagaaaag cgcaaaaagt cagaaataaa
     4441 aaagagaatg gacgggtcaa gataacctga cctgtccact tattttgttg ggctacaaag
     4501 acttcttttc ggaataagtg ggacatttat tattatttct taaaaaaaat ggatagaaaa
     4561 acaccaataa ttaaatactt atgcctaata acataaattt gctattgatt gatgtttcta
     4621 ttttaattac attaaattta accaataatc cagaaatggt tgcagctaaa atgcaaaaga
     4681 cagtggcgta aatggtttca gtattcacaa tttttctctt tcaaaattgt tcaatctaaa
     4741 tagaaaaagg taacttcaat acatattgct gtaactgaaa ttaccttttc cttaatgatg
     4801 taattatctt atggcttcat catgccacga ccgatattgt atccgccagc aacagagaaa
     4861 ataccagctc ccattgcgtc aacccagcct actggtcctg tccacttgtt ccgttgagct
     4921 acaaagactt ctggtctgaa aaatgagtga ttgttagaca tgtgtactta tttatgtttc
     4981 tttgattggg ataaatttgt aaaaacaaaa aaaaggcaag ttgttattat agaagaactt
     5041 aaaatgctta acatactcat tgaggtttgt accacataat gagatacgag ataatttgtt
     5101 gggagtaaaa tcaaaaaaaa gattaaatat ttaaaataat cactatattt tttcattatt
     5161 tgttcctttt tgtatttaag ttcttatgaa aaactagggt attttgaata gaaaatatca
     5221 aaatacccta gttttattaa ggaatattac caccaacaag tgcttccata ttgtacagaa
     5281 ccagtgattg caccaccaat agctccgaat ttcgcacctg gaatagcacc aataccacct
     5341 gcaaatgaac caacaattgc cccaccagct gctccaccta atgcactacc aatggtattt
     5401 tttgagaaat cacgccagtt acaagcagca ccagaaactt gttgaatttc atccaaagtc
     5461 aaaacttgca taatattcac ctttgttaag tgtggttgag aaaaaagtaa attttcatcg
     5521 ttactcatta tttgcaatca tgcatatact gtaaccaagc actatattca gttggatcat
     5581 agtttttact actgggttgt ctgatatgac tattggcact attggcacaa ctgggattag
     5641 caactttatt gtgagtacct aatggattga tatttcctgt aaagtggcta ttgcatgcgc
     5701 ttaatgagca tagatttatt aatgtcagaa aggataaaac aaaaaatctt accatttttt
     5761 actaaaacca aaagtaggtt tcgaaaactg tacaccaaga ccttgtacat atccaaattg
     5821 cttgctaaca gaaaactcta ctccactatt gttaggggaa taggaaacac caattttacc
     5881 aacagcaacc atatcttttt tacctaggtt gccgccaccc tgtgcagaga tatggaattt
     5941 gccacctgac acttcaacta attcagaggt gtgtaactct ttcatgattt tctcctgtaa
     6001 agaagtatgg ttggaaatgt aaatcagaat caatatttct taagtttaat ttaatacttt
     6061 tttttgcaag tattttattt taaattaaat caatatttta aaaatattta cttgtatact
     6121 gaaaaagaaa ctcctaatgg cggagtgggg cttttgttta agtaagcatt gaaaaaattt
     6181 aaatttccca ttatgattaa cttaatgcaa gagctttttc aggacgtaca aaccagcgaa
     6241 tgatgtgggc aggttggttg tcgctaacca atttcagtag ctcaatcgtc agattggtat
     6301 ccgaccctat caagctaagg agaggctgtg gctactacac aaagcacctg aaattattgg
     6361 gtgcttttgt ctttttatgg cattgtacca atttgtgtac cacgagggct tgttcgccca
     6421 aacgcggtaa caactgaata cacactttcc ccctcaactt ctttgcgttc taaaacgact
     6481 gtcccaatcc gagaatagac aatcatagga cgatgattgc cccttacatc actaaattca
     6541 ctaaaaattt ttgcgcctga ttgaaaaatc atttccacat aaaagggtta cacctgtttc
     6601 atcgtggctt aatccaaacc gcgttaaatc tgctttgtgt tctgccaaaa tgtgaatcac
     6661 accaaagccg tgattaacct ttttgtgttc gccgtgtttt aaaacaattt gacctgccgg
     6721 gaagtctgct ctattgttcg gacaataagg ggaatatacc aattacttta actacatcat
     6781 tttttgacat ttttcttgcc tttcaattta aaatccaaaa tcatactgcc ataatttagc
     6841 aatccaaaaa aatttaggca gcagtaatgg tttcgtattt tagaaaacga aacttggttt
     6901 ggtgttttga agcatttcag tacacaaaca tggaatttta agcggtttga gtattcggta
     6961 ctgtaaccgt ataaacctta ccatttcgct tcaaaagacc gaatacggcg actttaccgg
     7021 cagcaccgcg accgcgtttg cctttgcgtt gtccgccaaa ataactttca tctgcttcta
     7081 cttcgccatc aaacatttcc aaatgcggac tgttttgata aataagtaat cgtaaacgat
     7141 gaaaataata ggctgcggta tttttattaa cgcctactaa ctctgctgcc gttcttgcag
     7201 ttacacctgc gacaaacagt tcaatgagtt tattttgttt ataccggctt agacgacttt
     7261 ttctcatagg gataattcta acttaatttg aatttcccta gttatctagg acagccccaa
     7321 aataaattta gcctaaatca taaaaccgtg taattttgca ccaaaaaagg cggtttgcag
     7381 gtaaaatatc aaaccattaa tttaaatcat atttttacag aatattccgc cgcgttcacc
     7441 aaatggacat caaaccggtt ccaaatttct gtaattttgc aacaaaatac cgcaaaacac
     7501 ccgattgaga ccgaaaggac tttcatatga accagacaag ccgcgatctg acccgcatca
     7561 gccacaacac caaaatcgtc gccacccttg ggccgggcag caacaacgtc gaactgttgg
     7621 aagacatgat ccgcgtcggc ggcctgaacg tcgtccgctt caacttcagc cacggcacgc
     7681 ccgaattcca tcaggaaaac gcccgcatcg tgcgcgaggc ggcaaaacgc gccggacagg
     7741 aaatcgccat cattgccgat ctgcagggcc cgaaaatccg cgtgggcaaa atcgccggcg
     7801 gcggcatcga attgaacaaa ggcgaaacat tggtactcga tgccgcgctc gaaggcgaag
     7861 gcacgcgcga ggcggtcggt ttggactacc gcgacctgcc cgacgacgtt gccgcaggcg
     7921 atgtcttgtg gctggacgac ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca
     7981 ggattatcac aagggtggaa aacagccaca tcctgaaaag caacaaaggc atcaacaaac
     8041 gcggcggcgg tttgtccgca ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg
     8101 cgattgccat cggttgcgac tatctcgcca tcagctttgt gaaatccgcc gaagacctgc
     8161 acatcgcgcg cgccaaagtc gaagaggaaa tgaagggcag cactgccgtc cgccccggtt
     8221 tggtttccaa aatcgaacgc gtagaagcga ttgaaaacct tgatgaaatc atccttgccg
     8281 gcgacggcat tatggttgcg cgcggcgact tggcggtcga agtcggacac gccgccgtcc
     8341 ccgccctgca aaaacggatg atccgccgcg cccgcgagtt gcgccgcttc agcattacgg
     8401 cgacgcaaat gatggaatcg atgattacca accccgtgcc gacccgcgcg gaagtcagcg
     8461 atgtggcaaa cgcggtattg gacggcaccg atgcggtgat gtgttccgcc gaaaccgccg
     8521 tcggcgcgta tccgtttgaa accgtcagcc aaatggcgat tatctgcgcg gctgcggaaa
     8581 aagaacagga ttcgctcaac ggcgttgccg aacagacaga gtatcccgaa gccgtcagca
     8641 ccaacttagc gattgccggc ggcgcggtca gcgtggcgcg cgcggttcac gccaaagcca
     8701 tcgtcgccct gaccgaaagc ggttcgaccg cctttgaaat cagccgccac aatattaccc
     8761 tgccgatttt cgcgctgacc ccgagcgttt ccgcccaacg ccgtatggcg atgtaccggg
     8821 gcgtgcgccc gctgattttg gcaaccagca ccgaccacga cacggcgttg aacgaagtcg
     8881 aaacgatgct ggtggaacac aacatcctgc attccggcga ccagtacatc atcaccagcg
     8941 gctcgcaaat gcgcgaatcc ggttcgacca atacgctgga agtgctgcgc gtcaaataat
     9001 ccgccctgag tggaaaatgc cgtctaaagc cgatactgtc gggcttcaga cggcatttta
     9061 tttgcattcc gtcaggtaaa accgaaagaa ttgtgcaaat ccggcaacat cggataaaat
     9121 cgagtgccta tactaaagcg aaacaaggca tttccgactg ccttttttat ttgtccaccg
     9181 tccgcctttt tacggaaacc gaaatgaccc ctttcacact gaaaaaaccg tcctgctgct
     9241 cggcactgcc tttgccgccg catctgtcca cgcatccggc taccacttcg gcacacagtc
     9301 ggtcaacgcg caaagcacgg caaatgccgc cgacgcgtcg accatcttct acaatcccgc
     9361 cggcctgacc aaactcgaca gcagccagat ttccgtcaac gccaacatcg tgctgcccag
     9421 cattcattat gaagcagatt ccgccaccga ctttaccggg cttcccgtcc aaggttctaa
     9481 aaacggcaaa atcaccaaaa ccacggtcgc accccacatt tacggcgcat acaaagtcaa
     9541 cgacaatctg accgtgggct tgggcgtgta cgtccccttc ggctctgcca ccgaatacga
     9601 aaaagattcc gtgttgcgcc acaacatcaa caaactcggt ctgaccagca tcgccgtcga
     9661 acctgtcgcc gcgtggaaac tcaacgaacg ccattccttc ggcgcaggca tcatcgccca
     9721 acataattcc gccgaactgc gcaaatatgc cgactgagga atcccaaaaa aagcgcaaat
     9781 gctgcaagca acaccttcta atcctactgc cgctgctcaa atcaaggccg acggacacgc
     9841 cgatgtcaaa ggcagcgatt ggggcgtcgg ctaccaactg gcgtggatgt gggacatcaa
     9901 cgaccgcgcg cgcgtgggcg tgaactaccg ttccaaagtt tcacacacgc tcaaaggcga
     9961 tgccgaatgg gcggcagacg gcgcggcggc gaaacaacag tggaatgaca atatgctcac
    10021 accgctcggt tacacggcga atgaaaaagc ccgcgtcaaa atcgtaacgc ctgagtcttt
    10081 gtccgtacac ggcatgtaca aagtgtccga caaagccgac ctgttcggcg acgtaacttg
    10141 gacgcgccac agccgcttca ataaggcgga actgtttttt gaaaaagaaa aaaatattgc
    10201 taatggcaaa aaatccgacc gcaccaccat cacccccaac tggcgcaaca cctacaaagt
    10261 cggcttgggc ggttcttatc aaatcagcga accgctgcaa ctgcgcgtcg gcatcgcttt
    10321 tgacaaaccg cctgtccgca acgccgacta ccgcatgaac agcctgcccg acggcaaccg
    10381 catctggttc tccgccggca tgaaatacca tatcggcaaa aaccacgtcg tcgatgccgc
    10441 ctacacccac atccacatca acgacaccag ctaccgcacg gcgaaggcaa gcggcaacga
    10501 tgtggacagc aaaggtgcgt cttgcgcacg tttcaaaaac cacgccgaca tcatcgacct
    10561 gcaatacacc tacaaattca aataaacgtt acaccgtttg aatataaaaa tgccgtctga
    10621 aacagatttc cgtttcagac ggcattttta tccggcagcc gatacgccta tctcggtatt
    10681 cgggagaata tatacagaac ctccttcata tcaaaatggt agggacgctc gggcaacagg
    10741 acggcggcag gcagcttggt gatgccgttc aaaccgcgcg ggaacagcgc aatccgctct
    10801 ttgccctttt ccgcataatc tgctttgcgg ttgtggatgg aaactacttc gcgatggcgg
    10861 taaagcagcg tgttcatttc gatataggct tcgttgtgga tggctgccga ctgtttctgc
    10921 aacacgtcaa tctgcctgtt gagcttgtcg ttggctttca tcagcctgtt gaagaacgac
    10981 ggttgctcct gcagtttggc ggtacaggct tcgatacttt ccgataccgg cccgaactgt
    11041 tcgaggtatt tttccagaca gttttccaag tcggtcaggc agccggccat cttgcgggaa
    11101 aattcttctt caggctttcc ctgccgaatg gacaatatag cggattaaca aaaatcagga
    11161 caaggcgaca aagccgcaga cagtacaaat agtacggcaa ggcaaggcga ggcaacgccg
    11221 taccggtttt tgttaatcca ctatatttgg gaggggcgta accccttccg aatcaggatg
    11281 gcacataggg cgacgcttta tgtgccgtcc tgtgtgttga aacatcagca taggaaacac
    11341 aggaaaagcc ggctgttttg cgcctgcgta ccgtattggt caaaatgccg tctgaaaccg
    11401 acaacagggt ttcagacggc atttccgtca attttccctt tatctgccga acaggttggc
    11461 gatattttcc atctgttctt cggtaaaacc gttttcgccc aatctggcgg caactgccca
    11521 ttcgcccgac aaaccgtggt ccgcaccgag ctgccgccat ttttcgactt tggcttcggc
    11581 tgtggttttt tcgggcagcg gtttgacggg aatcctgctt ttggggacgg tattcatcgg
    11641 tttgccctaa tcgatggaat agccgcgaag atactgtatc cgttcgcgcg tcatccgcgt
    11701 gtcgcattgg agcttgaggt attcggcgta ttcctgccgg tctgcctgcg cggcggcttg
    11761 tcggcagttg ctgatttttt cctgcgccca cttgcgctgt tcgccgacca actctttttg
    11821 cacgtcggta tcgagtcctc cccaaagttt ggtaatttcg gattccgcac gttggttttg
    11881 tacgcgcgcc tcttccactt cgccccgtga tacggtaacg gtatcggcac gctcgacgtc
    11941 gtcgggatgc aggatttcgg gttcgggcgc gccttctgcg gcttggggta cgcccgcatc
    12001 gccgccggcg gcattgtgtt ccaaaatgtc ttcgggggtg ggtttggacg gttcttcttc
    12061 acgggctttg ccgctcaaaa ccctgaccgc gtcttctttt gtcaccgcct tgccgtctat
    12121 catcacgatg ctcttcacgc cgtaaggcag caacgcggca gacagcgttt gcgtcgccat
    12181 accgaccgtg ttgtcgataa atgccgtccg agcgtctttg gcgggcagga agcggacggc
    12241 tgccgtcaat acgccgtctt taaactcgac attgccgccc gtcttctgct gcacgatgtc
    12301 tgccaaagac gtttccccat acagcagggg gctgtttgcc tcggcatcgg caagcgtttc
    12361 agacggcacg gtaatgttca aatcggcgat acagaacgtg cgcccgcctt cctgcgtttc
    12421 cgaagcgtgt tccaaagaaa acgccaaacc gtaggcggcg gcgataattt tgtcggcatc
    12481 gacaaactgc ctgccgtctt cgcgcgcgaa agaacgcgct tcctgcgtga gcgtttcctg
    12541 aatactgccg cgtatgtcct gcaacacggc ggggttggcg cattccaacg ccttgggcgg
    12601 ttcttccctg ccgcacgctg caagcagcag ggcaaacggc agcgcaatga gtttccgata
    12661 catgatgttc cttccaaacg gtgcagaccg atggcggcta tcatagcaaa agcacctgca
    12721 ggcggtaaac cttgcaggcg ctttgtcgga aaggcggaaa atcagccttt aaagaaattc
    12781 acaaaaccgg tgagaatcgc ggcattaatc aaatcgacaa agaacgcgcc gaccataggc
    12841 acaatcaaaa acgccttatg cgacgcgccg aaagtatgcg tgacggactg catatttgcc
    12901 accgccgtcg gcgttgcgcc caaaccgaaa ccgcagtggc cggcagccaa tactgcggca
    12961 tcatagtcgc gccccataaa gacataggta acaaaagtcg cgtacaaaac catcactgcg
    13021 gtttgtactg caagaatcac ggttacagac cccgccaaac cggtcagctc ccacagtttc
    13081 aaattcagca acgccattgc caagaaaagc gaaagcgaag cattgccgaa cacatcgatg
    13141 gcacggtcga acatattgac cttgaatgcg gcggtaagga tgttgcggat aaccacgccg
    13201 ccaaacagac accacacgaa tttgggcagg tcgaacaggt attctttgtc gaagccgtcc
    13261 ataatctcgg caaacgccag acacgcggca aacatggcaa gcgtttcaac ggcagattcc
    13321 gccgtaatca ggcgggtgcg ttttgcctgc tcgaacacgt cgtccgcgtt gtcgtcctga
    13381 tcctgttttg tgttttcaac cggtttgcgg cccattttgt tgatcaggcg gcgcgcaacc
    13441 ggcccgccga tcaggccgcc gaacaccagc ccgaaagtag ccgaagcaat acccaaaccg
    13501 gttgcgccga ccaagccgta ttgcgtttca aaattaggtc cccacgcacc tgacgtgccg
    13561 tgtccgcccg tcagcgacac cgaaccggta atcagaccga tgagcgggtc caaacccaaa
    13621 gccgtagcca gtccgacccc gacaaagttt tgcaccaaga taaatccgcc cacaatcgcg
    13681 gtaaaaacca ccagcggcaa accgcccgcc ttcaaacggg aaaaatccgc gctcaagccg
    13741 atggacgtga agaaaatcag cataaacgca ttttgcagcg gtttctcaaa tttgaagctc
    13801 acgccgtacg cctcgtgcag cgcaaacagg ataatcgcgg caatcagccc gcccgccacg
    13861 ggttcgggga tgttaaaatc acgcaagatt ttgattttct taaccaaaac cttgccgacc
    13921 aacaaaacca aagtggcggc aatcaaggtg taataactgt taaacgccca ttccatatcg
    13981 tttgacctcc taaaatgttt ttctttatat ccgcccgaag gggacgcaag cgcgaagata
    14041 ttaggggaat agttaaggct tgtcaaagaa tcgccgtttt ttatagtgga ttaaatttaa
    14101 accagtacat cgttgactcg ccttgccgta ctatctgtac tgtctgtggc ttcgtcgcct
    14161 tgtcctgatt taaatttaat ccactataca actatttttt acaaacaaaa tgccgtccga
    14221 aactccgttc agacggcatc atgggaatac acatatttga aataacagaa aaagcggggg
    14281 caaaacccgc cgctgcatct gtcgctgtac atccctgctg tcggacttac cgcttcaact
    14341 tcgcaaacgc atccgccatc gccgaattgg ttgggacacg ctcgttgcgt tgtggtttgc
    14401 ggccggctgt ccgttcgcgg ctgcggtttt tccagctcgg acagaatgtc cagaatggtc
    14461 ggcagtccga agtgttcgtc ggtgaagtcg gacgctttga tttgcttcac gcgctcgcgg
    14521 ttaactgcct aaatatgaaa ttcttggtct tcttgtagca taagttctcg atgatatgcc
    14581 aagtaagatt tacgtttgtc gttgagcaga ttgttatcta gcttcacatc acaccaacgt
    14641 cgccaaacat catctgataa ccgcttagat ttcaccatat ttccttcatc atcaaacgaa
    14701 atatatttca aatcaaatag gctatccaag gtacgactta atagcaagcc gttatttggg
    14761 tcgtatgctt cggtatcatc cgacaggatg aaaggcttaa tatgactggc aatcaataca
    14821 ggataggcaa gtttttccag catacatttc acattaccgt aatgttcttc actttcttct
    14881 tgaagttggt ttttgtatag gcgatgtaaa taagggtcgc gtttccttac agtaatttca
    14941 tccaaattac caaaaatacg ttgggcatcc tcagcaaaat ataagtcatc acctactcgt
    15001 tttaagtcgt ctaatttgtc taataggttc cataaataac ttatttgatt gtattttcgt
    15061 tcaataaatc ctgattctat tccctgttga aaataatcat tcaattctgt ttcggttaga
    15121 taatcttgtt gaaaggtttt taagtctact aacatcatgg cggcaatttc ttttttattt
    15181 agcttgcctt gtggatgctc gaccagcgtt ttaatcagga aatttatttg tcgaatatta
    15241 gagttttcag ttactgaacg ctgaaagcca gagtgcgtat atacgatttt ggaaagtagg
    15301 gtttgtcgtt ttcggttagt acgagcttgt acataatctc gtgataaagg tgtatagctt
    15361 gataaaaacg gttctataaa tcccattttt acaaattgat taatagcttt acgaaccgag
    15421 gccaaattca tggccttgct tgttctctgc gaagccataa cggaatcttg ggcaatgttt
    15481 tgctgcaatg cttcatatag ttcgttgctg tatggctggt cttgattcgc atcaataaag
    15541 tcaagacaaa tattgagtgc agtacaaaat ttttgatcgt tataatttgt aaaggcattg
    15601 gtaaacgccc aataatcttc ataaatttgg ctgtttggct gattattact catctctgtc
    15661 tccaagaata ttcgatacca atattctgtt tttgtattta agtaatatct atcattcatg
    15721 tattttctac taaaaacaga atttccttat ggctttgctt cttttctttt cctgtaacct
    15781 tataaacgtg aggcgtttcc aagatttgta catttcgccc atttttacct aacatttctt
    15841 ctaattcatc tcggtttgga tatgaagcat tgttgtagct taacagccaa tatttaaatg
    15901 gtttcaaatg attgattacc cttttaaata attcgacagc ctgcttttta tccatgaaat
    15961 gattggcaaa tggtttgggt atggatgatg taatgtaact gtccaataat ccgtaaaagc
    16021 caaaataatt gttcatcgtg cctgtgtaag gtgggtctaa atagacggca tcagcttgta
    16081 tattaggtaa taaatcaaat atatcttgat taaaggcgtg atgccgccag ccattattga
    16141 aaacagcttg gttatagtca tccaaatttt gcagaaaatg actttgaaaa ctttcattgt
    16201 ggtaggcgcg tcgacggcca tatttagcat aactgtattc ttcatcacgc agttgtttaa
    16261 ctttttccca aggaattgtg aatcgtgaat agggcatttt acgtatcatg gcgcgacgca
    16321 ttaaagtaaa cgccaaagca cgtttatact ggttattcag cttccctata tttttacggt
    16381 acaaatcaag ttgttggcat tcatcgtgaa aatagaattt ttcggaataa cgctgactca
    16441 taaaaccggc atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca tttaatgttt
    16501 catggttgtt ttctattaat gctaaagcaa tttggtaatt aattgccaaa atatcgttag
    16561 taatcacacg atagccgcgt tttttggctt cgtaggcaaa ggaacagcct ccactaaata
    16621 catctgcaac tgtatctaca tcagacggaa gctggtcaca aatccaggaa gctattttct
    16681 ctttattacc gatatagtta attttcggat atttcttatt cattttcact catttttaaa
    16741 atactttcag caatagcttt tgccaataaa ggcggcacgg cgttaccaac ctgctgctgc
    16801 tgggcaattt tgctgccgca aaaaataaaa ttatcaggga aagattgtaa ggcagctaat
    16861 tcacgaacgg ttaaagcccg attctgttca tagtgaaaaa ctttgcgcat atctcctgta
    16921 atacaaacgg ctggtttgtt gctgttgtaa cggatgtatt tacggatatc acctgttttc
    16981 ggacgtaatg gttcaggaat atcgttacgg ttacctccat ttttaacaaa tgccattttt
    17041 tctaacattt gtgccgaatg attcatagct tcatgatttg caacgtgtgg attgctttcg
    17101 ccagcagcca gttttggaaa atgtcctatt gctgatccaa cagtctgatg ggaaatctgc
    17161 aaaggttcgg gaaaggaaat tttgccttta tccctcctcc cgataaatat cactcggcta
    17221 cgtatctgag gaacaccgaa atcggctgca ctcagtatct tacattccac cgaataaccg
    17281 atattctgaa atgcttgaat aatctcaata cgtgttttac ctgaattgtg tgtatagagt
    17341 cgcgctacat tttccataac aaaaaaatat ggttggacaa ttttaactat tcggacaaac
    17401 tctttaaata aatggttgcg tgggtcatct gtaaatgtcc gtccaatctt tcctgccata
    17461 ctaaaacctt gacaaggtgg tcctccaata accaaatcaa ctgattgtcc gttaagacaa
    17521 ttggttaaat cttgttcggt tagtgtggtt aaatcttttt gcagtaattg atgacggggg
    17581 aagttggtac ggtaagtctg acaataatca gactccattt caacagaaag caattgttgg
    17641 aatccggctt gttcaaaacc caaggatagg cctcctgctc ctgagaaaag gtcaatatag
    17701 gtaagtggtg tgtgcataag tcaaaatcca taaactcttc gattatttta ccgcttcaac
    17761 ttcgcaaacg catccgccat cgccgaattg gtcggggcgc ggtcgttgcg ttggggtttg
    17821 cggccggctg tccgttcgcg gctgcggttt tcagacggca ttttatgttt tgcgccgccc
    17881 ggttcgtcat ccaagcgcat ggtcagcgcg atgcgtttgc gtgcggcatc gacttccagc
    17941 actttcactt ttaccacgtc gccggctttc accacgtcgc gcgggtcttg gacgaactta
    18001 ttagacaggg cggagatgtg caccaagccg tcctgatgga cgccgatgtc cacgaacgcg
    18061 ccgaagttgg cgacgttgga gaccacgccc tcgagtatca tgccgacttg caagtcgctg
    18121 atttcgtgga tgccttcggc aaacgatgcc gtctgaaacg caccgcgcgg gtcgcggccg
    18181 ggtttttcca gctcggacag gatgtccaaa atagtcggca gaccgaagcg ttcgtcggtg
    18241 aagtcggatg ctttgatttg cttcacacgc tcgcggttgc cgatgagttc ggcggcggta
    18301 atgccttgtt gcgccagcat tttggcgacg acgggatagg cttcggggtg gacggcgctc
    18361 gcgtccagcg gctctttgcc gccgttaatc cgcaaaaagc ctgctgcttg ctcgaaggtt
    18421 ttttcgccca agcgcggtac ttttagcaat ttttttcggc tgtcgaacgc gccgttttca
    18481 tcacggtagg cgacgatgtt ttgggcaagg gtttgattca agccggaaat ccgcgccaag
    18541 agcggggcgg aggcggtgtt cgcgtccacg ccgacggcgt tcacgcagtc ttcgaccacc
    18601 gcgtccagcg atttggcgag ccggctttgg ttcacgtcgt gctgatactg ccccacgccg
    18661 atggatttgg ggtcgatttt gaccaactcg gcaagcgggt cttgcagcct gcgggcgatg
    18721 gacactgcgc cgcgcaggga aacatctaag tcgggaaact cgcgcgccgc cagttcggac
    18781 gcagaataaa tcgacgcgcc ggcttcggaa acgacgattt tgtgcaggct gctttcgggc
    18841 attccgcgca ccagttcgcc cgcgattttg tcggtttcgc ggctggcggt gccgttgccg
    18901 atggcgatga gcttcacacc gtgttgttta atcaggcgcg acaacgttgc caacatattg
    18961 ttttcttgat gcaaatagac aatgaccgta tccagcagtt tgcccgtgtc gtccaccacg
    19021 gcgcatttca cgccgttgcg gtagccgggg tcgagtccca aagtggtcag ccgcccggcg
    19081 ggcgcggcga gcagcaagtc tttgagattg cgggcgaaca cggtaatcgc gtcagtgtcg
    19141 gcagcttctt tcaaacgatt gagagcttcg agttccagcg acaaaaagat tttcgcgcgc
    19201 caagtcaggc gcacggtgtc gcgcagccat ttgtggccgt ctgaaacctt gaagcggcgg
    19261 gcgatgattt gctcgtattc gctttgctgc gtaatcggcg tgtcgtcggg ctggtatttg
    19321 agcgcgatgt tcaacacgcc ttcgttgcga ccgcgcaaaa ccgccagcgc gcggtggctg
    19381 ggcatggcgc gcacgggttc gcggcggtcg aaataatcgc tgaatttttc gccttcggtt
    19441 tctttgcctt caacgacttg cgtgtggatt tcggcttcgt tccacagctt gtcgcgcagc
    19501 gtgccgatga gttccgcgtc ttcggcaaac tgctccatca gaatcgcgcg cgcgccatcc
    19561 agcgcggctt tggcgtcggg gatgttttcg ttcaggtagc cttgcgcggc ggcttccacg
    19621 tcctgcggct gttcggcaag caacacgtcc gccagcggct gcaaaccgtg ttcgcgagcg
    19681 atttgcgctt tggtgcggcg tttgggtttg tagggcaggt acaggtcttc caacgcggtt
    19741 ttgttgtcgg cggcttcgat ttgcgccctg aggtcgtctg aaagcttgcc ttgctcttca
    19801 atgcttttta acacaacggc cttgcgctct tccaactcgc gcaggtattg cagccgctcg
    19861 gcaagctggc gcagctgcgt atcgtccagc ccgcccgtgg cttctttacg gtaacgcgcg
    19921 ataaagggga cggtcgcgcc gtcgtccaaa agctcgatag cggcattgat ttgcacggta
    19981 gtggcggaga gttcttggga gaggattcgg gtgatgttca taaattgcct ccgtgatgcc
    20041 gtctgaacag ccgacggcgc aatgtgggta atcgtttgga aaagatgaag ataatactga
    20101 tatactttgc ccgatacaat ctgaaaggaa tgtttatgac cgtcctgatt accggcggca
    20161 ccggctttat cggttcgcac accgccgtct cgctcgtcca atccggttac gatgccgtga
    20221 ttttggataa tctgtgcaac tcgtctgccg ccgtcctccc gcgccttcgg caaattaccg
    20281 gcagaaacat accgttttat cagggcgaca tccgcgactg tcagattttg aggcagattt
    20341 tttcagaaca tgaaatcgaa tccgtcatcc attttgccgg tttgaaggca gtgggggaaa
    20401 gcgttgccga gccgacaaaa tattacggca acaatgttta cggcagcctg gtgctggcgg
    20461 aagaaatggc gcgcgcgggc gtgttgaaaa tcgtattcag ctcgtcggca accgtttacg
    20521 gcgatgcgga aaaagtcccc tatacggaag atatgcgccc gggcgatacc gctaatcctt
    20581 acggtgcgtc caaagcgatg gtggagcgga tgttaaccga catccaaaaa gccgatccgc
    20641 gttggagcgt gattttgttg cgctatttca acccgatcgg cgcgcacgaa agcggactta
    20701 tcggcgaaca gcccaacggc gttcccaaca atcttttgcc ctatatctgt caagtggctt
    20761 cgggcaggct gccgcaactg tcggtattcg gcggcgacta tccgaccccc gacggtacgg
    20821 gaatgcgcga ctacatccat gtgatggatt tggcagaagg gcatatcgcg gcaatgaagg
    20881 cgaaaggcgg cgttgccggc gtacatttgt tcaacttggg ttcgggacgc gcctattccg
    20941 ttttggaaat catccgcgcc tttgaggccg catccggttt gcacattcct taccgaatcc
    21001 aaccccgccg cgccggcgac ttggcgtgtt cctatgccga cccgtcccat accaaacaac
    21061 aaaccggctg ggaaaccaaa cgcggcttgc agcaaatgat ggaagattcg tggcgttggg
    21121 tcagccgcaa ccccggcaga tatggggatt aaatgcaaac cgcaggcaaa aaaaacatcc
    21181 tcgttaccgg cggcgcgggt tttatcggct cggcagtcgt ccgccatatt atccaaaaca
    21241 cccgagattc cgtcgtcaac ctcgacaagt taacctacgc gggcaatctc gaatcgctga
    21301 ccgacattgc cgataatccc cgctacgctt ttgagcaagt ggatatttgc gaccgcgccg
    21361 aactcgaccg cgtgttcgcg caataccggc ccgatgccgt gatgcacttg gcggcggaaa
    21421 gccacgtcga ccgcgccatc ggttcggcag gcgaatttat ccgaaccaat atcgtcggca
    21481 cattcgacct gctggaagct gcgcgcgcct attggcagca aatgccgtct gaaaaacgcg
    21541 aagccttccg tttccaccat atttctaccg atgaagtcta tggcgatttg cacggcacag
    21601 acgatttgtt tacagaaacc acgccatacg cgccgtccag cccctactcc gcctccaaag
    21661 ccgccgccga ccaccttgtc cgcgcgtggc agcggactta caggctgccc tccattgtca
    21721 gcaactgttc caataattac ggcccccgac aattccccga aaaactcatt cctttgatga
    21781 ttctgaacgc actttccggc aaaccactgc ctgtgtacgg cgacggggcg caaatccgcg
    21841 actggctgtt tgtcgaagac cacgcgcgcg cgctgtatca ggtcgttacc gaaggtgttg
    21901 tcggcgaaac ctacaatatc ggcggacaca atgaaaaaac caatctcgaa gtgatcaaaa
    21961 ccatctgcgc cctcttggaa gaactcgccc ccgaaaaacc ggccggcgtg gcacggtatg
    22021 aagatttaat tactttcgta caagaccgcc ccggccatga tgcccgctac gccgtcgatg
    22081 cggcgaaaat caggcgggat ttgggctggc tgcctttgga aacttttgaa tccggcctcc
    22141 gcaaaaccgt gcaatggtat ttggacaaca aaacccggcg gcaaaacgca taaaacggca
    22201 actgccgttc ggaacgctta ggtacgggca aatagttttc agacggcatc ccgacgcaat
    22261 gccgtctgaa aacccatcgc aaaggaagaa ggaaaagatg aaaggcatca tactggcagg
    22321 cggcagcggt acgcgcctat atccgattac gcgcggcgta tccaaacaac tgctgcccgt
    22381 gtacgacaaa ccgatgattt attacccctt gtcggttttg atgctggcgg gaatccgcga
    22441 tattttggta attaccgcac ccgaagacaa cgctgccttc caacggctac tcggcgacgg
    22501 ctccgacttc ggcatccgcc tgcaatatgc cgttcaaccc agccccgacg gcttggcgca
    22561 ggcatttatc atcggcgaag aatttatcgg caacggcaat gtctgcctga ttttgggcga
    22621 caatattttc tacggtcaat catttacgca aacattgaaa caggccgccg ccaagaccca
    22681 cggcgcaacc gtattcggtt atcgggttaa agaccccgag cgtttcggcg tggttgaatt
    22741 tgacgaaaac ttcaacgcct tatccatcga agagaaaccg caacagccca aatccgattg
    22801 ggcggtaacc ggcttgtact tccacgacaa ccgcgccgtc gagttcgcca aacagctcaa
    22861 accgtccgca cgcggcgaat tggaaatttc cgacctcaac cggatgtatc tggaagacgg
    22921 ctcgctgtcc gttcaaatat tgggacgcgg tttcgcgtgg ttggacaccg gcacgcaaga
    22981 aagcctgcac gaggcggctt ccttcgtcca aaccgtgcaa aatatccaaa acctgcacat
    23041 cgcctgcctc gaagaaatcg cctggcgtaa cggctggctg acgaaaaaag atgtggaaac
    23101 acgggcaaag cctttggaaa aaaccgccta cgggcaatac ctgctgcgcc tgatcggcaa
    23161 ataaataccg tctgaacgcc ccgacggctt tcagacggca tccaaaccgt gccgcgccgg
    23221 caccttaacc gacctgccgc acggcgcgca taaaaaatca aaccgcaaaa aaacagcggt
    23281 aacgataccg ccaaaggact ttatggacat catcgacacc gccctccccg acgtaaaact
    23341 tttaaaaccg caagtcttca cagacgggcg cggttttttt atggaaacat tccgcgacgg
    23401 atggttcaaa gaaaatattg ccgatcgaac tttcgtgcag gaaaaccact ccaactccag
    23461 caaaggcgta ttgcgcggcc tgcactacca aaccgaaaac acccaaggca aactcgtacg
    23521 cgtagttgtc ggcgaagtgt tcgacgtggc cgtcgatatg cgcgaaggtt cgccgacttt
    23581 cggcaaatgg gcgggcgcaa ccttgtcggc gcaaaaccga taccagcttt ggatacccga
    23641 aggcttcgca cacggttttt gcgttttggg cgacgcggcg gaagtcgtgt acaaatgcac
    23701 ggactattac aaccctgaaa ccgaacaggt tttaatatgg aacgacccgg caatcggcat
    23761 aggctggccg cttcaaaccg cgccgctgct ttcgcccaaa gaccttgccg gcaaaacgtg
    23821 ggcacaagcc gaaaagctcc gccttacgct ttcccgataa aaaatgccgt ccgaacgttt
    23881 cagacggcat tttctccgac aggctaccta cccgccttca gtacgcgctg tgcaaagaaa
    23941 aacatcccgg taacgaagaa cgccaagccc aaccaagaag cggcggcatc ccagttttgc
    24001 agattcaacg cgaacggcgc atccgagccg ccgttggtca cagagaaggc aataataaac
    24061 gccataatta caccaatcag gctttcaccg acaatcaggc ctgccgcgaa aagcgtaccg
    24121 atacggtcgg cgtttttcag acggccttcg cggttttccg ccttcttgcc aatgatgtgt
    24181 ttcaacaccg ccgccaacac cgcgcctgcc acgatgggca tattgacgga cggcggcaga
    24241 taaatgccca taccgaccgc aaggacgggc agggcaagtt taccgcctga tgattttttc
    24301 aacaccaaat cgacgacgat taataccgct ccaatcgcga taccggtaaa gatgtacgcc
    24361 cattcgaggt tgtgggcgaa aatgcccgac gcaatggtcg tcatcaaagt cgcttgaggg
    24421 gctgccaaag cctgcgccgc atccatgcct tcgcgcggca ttgcgccggt aaagccgtag
    24481 gcttcgtaaa gcagttccaa cacgggcgaa ataaccagcg caccaacgat acaaccgata
    24541 atcagggcga cttgctgccg ccaaggcgta gctttgagca ggtagccggt tttcaagtct
    24601 tgcaggttgt cgttggaaat cgaagccacg cagattactg ccgatccgca aaaaagcgtc
    24661 aacgccagta agaatttgcg gttggcctca tccgccaaca aacctccgga ttcgcctacc
    24721 agcagcaaaa ccagtgaaat aacgacgatg gacacgatgc ccacgccgga aatcgggctg
    24781 gaagacgagc cgaccaaacc tgccatataa ccgcaggcgg cggcgaccaa aaagccgatg
    24841 acggaagcca aaagcgtgca aaccgccacc aaaagccacg ccatgccgcc cgtaatgtgc
    24901 gaatcgccga taaagtggta aaacgacacg cctagaataa acatcataga caacacccag
    24961 aaaatcatgg ctttaggcga caaatcctgt tcggcgcgtt ctgtagcggg cgcaccgccg
    25021 ccaaaactct tgaacgacat cttcatgcct tccaccattg gcttgagcag catcaaaagc
    25081 gtccaaaccg ccgcgatacc tatggttccc gcaccgataa aacgcacttt ttccttccac
    25141 agcttcatcg caaacgccgc catttccata tcggaaggtt gcggaatgtg tgaggagaaa
    25201 tacggcacgg caatacccca agcaatcgaa atgcccagca ggatggcgat gccgcccgtc
    25261 agtccgacca aataacccgc gcccaacaag gccagtgaaa agcccatcgg cagttggaaa
    25321 attgccgtac cgcttttaaa ccaataactc gcgctatcgg caatcacacg cagacctccg
    25381 gcgcaaaagc tcatcaatcc cgccaacgcg ccgccggccg ccagctcttt gataccgctg
    25441 ccgccctgac ggtcatcccc ttcttcatga ccgcccactt tcaaaatttc agcagccgcc
    25501 acaccttccg gataaggcaa atcgcttttc accaccatcg cgtaacgcag cggaatggtg
    25561 aaaatcaccc ccaaaatccc gccggcaata cataaaagcg tcgtctgcca aaacgggaaa
    25621 ccgctccagt agcccgccat cagcaaaccg ggcaggacga agatgatggt tgaaagcgta
    25681 cccgcagccg aggcttgggt ctgcaccatg ttgttttcca aaatattgct gcctttgaaa
    25741 aattttaaaa ccgccatcga aatcaccgcc gccggaatcg acgaggcaaa ggtcagcccg
    25801 actttcaaac cgaggtaaac gttagacgca gtaaaaatta cagtgatcaa tgcgccgagt
    25861 atcatgcctc ggagcgtcag ttcgcggtat tcttctaccg aaccggataa agatttattc
    25921 attattcttc ctttgacaac agacgttcac atattgttgg catcacgcca tgatgtcaag
    25981 ttttaaaaag aacagttaaa aacaattatc ccaccctgcc catacccaat tgaaaataaa
    26041 aactatttcc aaacgaacaa aaacagccgt atcagggagg ttccccgata cggctgcttg
    26101 tttccgaacc ttaaaatcaa tcaaacaaat cgcgcagctt gtctaaaaac gatttcttgc
    26161 gcggcgtttg gttttccaag cccgtagaaa tccgctcaaa ttcttccaaa agctcttttt
    26221 gacggtcggt caaattgaca ggcgtttcga caacaatatg gcagtacaaa tcgccggtcg
    26281 cgctgctgcg taaagatttg acacccttac ctttcacgcg catcctcctg ccggtttggg
    26341 tttctttggg gacggtgagc ttgactttgc cgtccaaggt cggcacttct aactccccgc
    26401 ccaaagcagc cgtggcaaaa ctaatcggca gttcgcaatg caaatccaaa ccatcgcgtt
    26461 ggaaaatctt atgcgcccga atgcggacgg ttacatacag gtcgccggca ggcgcgccgt
    26521 gcatacccgg cccgccttca ccgctcaaac ggatacgctg cccgtcatcg ataccggcgg
    26581 gaatattgac ttccaccgtc ttgaccgcct tattccgccc cacgccacgg catttgacgc
    26641 aaggttcttt aatatgtttg cccgcaccgc ggcaggtcgg acaagtctgc tgcatacgga
    26701 aaatcgcctg ctggatgtgc accgtacccg aacctttgca agtcgggcag gtttccgggg
    26761 atgcccccgg tttcgcgccg ctgccgttgc agacatcaca cgcttcataa gtcggaatat
    26821 tgatgcgttt cttcacacct tttgcggctt cttcaagcgt gatttcgata ccgacctgca
    26881 cgtcctcacc ctgataatca ggctgggcgc gccccgaacc acctccaaac atttggctga
    26941 aaatatcccc aaagtcaaaa ccctgcgcac cgccaaatcc gccaaaccct ccgaagcccc
    27001 cctgtccgcc gccttcaaac gccgcatgac catactggtc gtacatggca cgcttttcct
    27061 tgtcggacaa agtttcatac gccttttgta cttctttaaa cttctcttcc gcctctttat
    27121 tgtcaggatt gcggtcggga tggtatttca tcgccaattt acggtaggct tttttaatct
    27181 catcatcggt agctgctctt gccacaccca gcgtcgcata aaaatcttga ttactcattt
    27241 tttcatctaa aaataaaatc acggcttaaa atgagggcaa ttgcgcaaaa cacaagacaa
    27301 acagactgag acctttgcaa aaatccccta aaatccc
//
LOCUS       DAWTCI010000019        25481 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWTCI010000019 DAWTCI010000000
VERSION     DAWTCI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25481
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.019"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            65..601
                     /locus_tag="WM481_000819"
     CDS             65..601
                     /locus_tag="WM481_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9080178.1"
                     /translation="MRTLPGWNASSPSKPTITKKKNLLHEIFSKSRIGDTELFAVDEN
                     LVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPY
                     ALPRLLADAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFSRADLEKAYS
                     EYLELFKSESDAGGRKPR"
     gene            637..1398
                     /locus_tag="WM481_000820"
     CDS             637..1398
                     /locus_tag="WM481_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFA9080179.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIS
                     ALDFAMVKVPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGGLTNVEQGESVCV
                     PVGDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDTAWGIDFGDPGQVLPVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAMAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            1454..1795
                     /gene="fdx"
                     /locus_tag="WM481_000821"
     CDS             1454..1795
                     /gene="fdx"
                     /locus_tag="WM481_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFA9080180.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPAELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(1855..2395)
                     /locus_tag="WM481_000822"
                     /pseudo
     CDS             complement(1855..2395)
                     /locus_tag="WM481_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            2608..2805
                     /gene="iscX"
                     /locus_tag="WM481_000823"
     CDS             2608..2805
                     /gene="iscX"
                     /locus_tag="WM481_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFA9080181.1"
                     /translation="MKWTDTRRIAEELYDLHGEAIDPKTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(2873..3274)
                     /locus_tag="WM481_000824"
     CDS             complement(2873..3274)
                     /locus_tag="WM481_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFA9080182.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPHKIKVKGLNHQRRPAPEARLLYEEDAKTAALREECKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            3486..4445
                     /locus_tag="WM481_000825"
     CDS             3486..4445
                     /locus_tag="WM481_000825"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFA9080183.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVVVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4513..5823
                     /gene="tilS"
                     /locus_tag="WM481_000826"
     CDS             4513..5823
                     /gene="tilS"
                     /locus_tag="WM481_000826"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFA9080184.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVCA
                     GKRAGFVPEALHIHHGLSPRADDWADFCRNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAEFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPLGENGIIWR
                     PLLPFSRQDIWDYARKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLEEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRNFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFASEQLAKPAFLKDGTISGNLK
                     KILTEHRFVLKRHPFGLPEAMLEQDGILRTVAASDTLAVGGIHKNVKKILQGKRVLPF
                     LRPIWPLVADSGNRPLALANCCADFQISVSDGILPVHPDFPILF"
     gene            5897..6682
                     /locus_tag="WM481_000827"
     CDS             5897..6682
                     /locus_tag="WM481_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFA9080185.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEAKMPSEEVLKLTAVLPEDGIFSVSDGILKKISSLSCADDILTLIDI
                     PLGGTLPDKGDCAVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLAHYEDRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRAAY"
     gene            6692..6889
                     /locus_tag="WM481_000828"
     CDS             6692..6889
                     /locus_tag="WM481_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080186.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILSSNAVAAAVLFWIWWKWF"
     gene            7151..7504
                     /locus_tag="WM481_000829"
     CDS             7151..7504
                     /locus_tag="WM481_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080187.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKYRLVVIANSAWGLLLNLP
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEKQ"
     gene            7516..7821
                     /locus_tag="WM481_000830"
     CDS             7516..7821
                     /locus_tag="WM481_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFA9080188.1"
                     /translation="MRVSKMIGSILLVAAVQTVFSANVYECRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEAVQNPKGNAQKDGSDAGIRPH"
     gene            7883..9259
                     /gene="mpl"
                     /locus_tag="WM481_000831"
     CDS             7883..9259
                     /gene="mpl"
                     /locus_tag="WM481_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFA9080189.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFKVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFQADIYVIGNVARRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SKSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGQQQSLQDTLDKGCWTPVEKFGTGHGWQIGEVNADGSFDVLLDGK
                     KAGHVAWDLMGGHNRMNALAVIAAARHAGVDVQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKSALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGCRLRVGKDFDTFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            9466..10518
                     /gene="bioB"
                     /locus_tag="WM481_000832"
     CDS             9466..10518
                     /gene="bioB"
                     /locus_tag="WM481_000832"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFA9080190.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVYQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTVSVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKADY"
     gene            complement(10614..11414)
                     /locus_tag="WM481_000833"
     CDS             complement(10614..11414)
                     /locus_tag="WM481_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFA9080191.1"
                     /translation="MTKAVRRKNVQTTKICYAFRTPSAYGQYLADAGFDYLSLANNHS
                     NGFGAQGITATAGNLDELNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDY
                     AKFKKLIRKTKQKTDIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDS
                     GADVVFGQGAHVTRAVELYHDRFISYSGGNFATYGISGIAPIFKIITDKQGRFVSGNI
                     IPITQVGDKIPKIDPEKTVIERIIYLNHSDFPNGNGLDVSPGGDITRR"
     gene            complement(11425..11727)
                     /locus_tag="WM481_000834"
     CDS             complement(11425..11727)
                     /locus_tag="WM481_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080192.1"
                     /translation="MIINKFIWAMAVFSAILALVIGSGFTALEYVKEPPAAPYAVSAP
                     KTAGVKPRRILPERNPPCCRLTVQTPRPFQTPAMRTDRIRLTERQQIPFPSSGSAA"
     gene            complement(11769..13628)
                     /gene="ilvD"
                     /locus_tag="WM481_000835"
     CDS             complement(11769..13628)
                     /gene="ilvD"
                     /locus_tag="WM481_000835"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFA9080193.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVYTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRIWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEDILGNQIVAGNIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTIEIDIPKRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVSQIER"
     gene            complement(13801..15566)
                     /gene="cysI"
                     /locus_tag="WM481_000836"
                     /pseudo
     CDS             complement(13801..15566)
                     /gene="cysI"
                     /locus_tag="WM481_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
     gene            complement(15593..16627)
                     /locus_tag="WM481_000837"
     CDS             complement(15593..16627)
                     /locus_tag="WM481_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HFA9080194.1"
                     /translation="MRHIEIDLSGSDLHYLPGDALGVWFDNDPALVGEILDLLGIDPA
                     TEIQAGGKTLPVASALLSHFELTQNTPAFVKGYATFADDDELDRIAADNAVLQGFVQS
                     TPIAGVLHRFPAKLTAEQFAGLLRPLAPRLYSISSSQAEAGDEVHLTVGAVRFEHERR
                     ARSGGASGFFADRLEEDGTVRVFAERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQ
                     RAAENAEGRNWLIFGNPHFAADFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDK
                     IREQAEGLWQWLQEGAHIYVCGDAAKMAKEVEAALLDVIIGAGHSDEDGAEGYLDMLR
                     EEKRYQRDVY"
     gene            complement(16632..17315)
                     /locus_tag="WM481_000838"
     CDS             complement(16632..17315)
                     /locus_tag="WM481_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFA9080195.1"
                     /translation="MSGLSGYAWAKAGNGASAGLPALQTALPAAEPFSVTVLSASQTG
                     NAKSVADKAADSLKAAGIQVRRAELKGYKAKNIAGERRLLLVTSTQGEGEPPEEAVVP
                     HKLLNGKKAPKLDKLQFAVLGLGDSSYPNFCRAGKDFDKRFEELGAKRLLERVDADLD
                     FAAAADGWTGRIVARLKEEAAKNRATPAPQATSPRRPSDGIGGQVLQGRPLSRRPAGQ
                     SENHRPPIR"
     gene            complement(17383..18465)
                     /locus_tag="WM481_000839"
     CDS             complement(17383..18465)
                     /locus_tag="WM481_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylate transferase"
                     /protein_id="HFA9080196.1"
                     /translation="MNYFPIFANLSGRPVLVVGGGAVAARKISLLPKAGAEVGVAAKH
                     LNAELSALAAENKILWLAKEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VGDRDHCSFIFPSVIDRDPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAGFYFPVQLVVRQDADKADGFRGYQGRIERGSVTVGQTVRIEPNGLTAEVSEIIA
                     PKGEVAQAFAGEAATIRLDRDIDVSRGDLFVDKNFPLAPQKHPEATLCWFDERPLNTA
                     RKYLLKHGTQTVSAKVGEIESVLDVRTLEQETGAESLKMNDIAKVRINLQKPVTATPY
                     AENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(18894..19550)
                     /locus_tag="WM481_000840"
     CDS             complement(18894..19550)
                     /locus_tag="WM481_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFA9080197.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LLVLFLLVFRLCWGIWGSDTARFSRFVRGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVH
                     IAAVAAYRIFKKKNLVRPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            19615..20505
                     /locus_tag="WM481_000841"
     CDS             19615..20505
                     /locus_tag="WM481_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFA9080198.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPHVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVRAGFNQDTLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDAAAVETVAAVLARNRDEAVVLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAILGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     SAGEIAPAARWLRNWITESM"
     gene            20622..22433
                     /gene="typA"
                     /locus_tag="WM481_000842"
     CDS             20622..22433
                     /gene="typA"
                     /locus_tag="WM481_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFA9080199.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGCHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLRVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(22830..22920)
                     /locus_tag="WM481_000843"
     tRNA            complement(22830..22920)
                     /locus_tag="WM481_000843"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22884..22886),aa:Ser,seq:gga)
     gene            complement(22959..23045)
                     /locus_tag="WM481_000844"
     tRNA            complement(22959..23045)
                     /locus_tag="WM481_000844"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23009..23011),aa:Leu,seq:cag)
     gene            23134..>25481
                     /gene="rnr"
                     /locus_tag="WM481_000845"
     CDS             23134..>25481
                     /gene="rnr"
                     /locus_tag="WM481_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFA9080200.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLMPMDEGDFVLYERQMRGVMHGDTVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGKIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDL
                     CDLPLVTIDGETARDFDDAVFAEKVGRNYRLVVAIADVSHYVRPDDAIDADAQERSTS
                     VYFPRRMIPMLPENLSNGICSLNPDVERLCMVCDMVVTYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGNPHKAQIDTLYKLFKILQKKRLARGAVEFESVETQMLFDGNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTSEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALAEQFKGRPDAELLQVMMLRSMQQAVYEPHCEGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTPNKSWQALGVHTSFCERRADDAGRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRRRKVKLSASAKPAG
                     AAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKPVPI"
BASE COUNT         5794 a         6541 c         7316 g         5830 t
ORIGIN      
        1 aaaatcggca tttcggacgt aagcaatttt gaaggcagaa tgcgccattt ggaaaacaac
       61 ggctatgcga acgttgccgg gttggaacgc ctcctcgccg tcaaaaccga caattacaaa
      121 gaaaaaaaac ctgctccatg aaattttcag caaaagcagg ataggcgata ccgaattgtt
      181 cgccgtggac gaaaaccttg tgaaacgttt gtttttatcg cttcgcggcg aaatcgtgtt
      241 cccgaaaaac gaaacggcgg aatcggaatt tgaaaaaagc gtccacgaac gccggcagga
      301 agggaatgcc gggtccggcc gcaaacaact gcttgatttg gtacggcgcg gacaccggga
      361 atacccttac gcgctgcccc ggcttttggc ggacgcggca tcctacaagc cgaaaaaatc
      421 gaaaatccgc ctttttaaag aagcatattt cggcaaaagc ggcacgaggc tgaccgacga
      481 aattgcagac ggcatccata tttacacctg tttttcgcgg gcggatttgg aaaaagccta
      541 ttccgaatat ttggaacttt tcaaatccga atcggatgcc ggaggtagaa aaccgcggta
      601 aggtgcaaac agataccgta cacgttgagg agcagtatga tgggcgattc cgtcatttat
      661 tatgtagaac aggcagacga accggtaaac cgtgccggcg aacgcgcccg taaaacattc
      721 aaatactttt ggcgcgagct tttttgggaa cgccgccgca ttatttccgc cttggatttt
      781 gccatggtca aagtcccttt tttccaagac ggcgaagacg gcgaaatttg cgaacatatg
      841 tggatcgacg atatttattt tgacggtctt tatatttacg gtgtgctgaa caatgaaccc
      901 ggcggactga ccaatgtcga acaaggcgaa agcgtttgcg ttccggttgg cgacatcagc
      961 gactggatgt tcgtgtgcaa cggcatcccc tacggcggct ttaccgtaca ggcaatgcgc
     1021 ggacagatga cggaagagga gcgtaccgaa cacgataccg cctggggaat cgatttcggc
     1081 gatcccgggc aggtattgcc ggtgtatgaa gaaaaagaac atcccgaaaa tttggaagag
     1141 catccgatgt gccggaactg tattgacgat tttcggcaac agttgtccca aaatccggat
     1201 tttctgcatg agcaggacga agacggctat acgccgcttc atcatgaagc catggcagga
     1261 aatgcactta tggttcaagc catgcttgaa tacggcgcaa atcctgcctc aaagacatcg
     1321 gaaggctata ccgccctcga ttttgcccgc ctgacgggct ggcaaaatgt tgccgacctg
     1381 ctcgaaccgc gacattaggc agacagtttt cctaaaaacg gacacaaata ctttttacag
     1441 aaagacaata aaaatgccca aaatcaccgt acttccacac gcaacattat gccccgaggg
     1501 tgcagtcatc gataacgcgc ccgaaggcaa aaccgtcctc gacgtgctgc tcgaccacga
     1561 catcgaagtc gatcacgcct gcgaaaaatc ctgcgcctgc accacctgcc acgtgattat
     1621 ccgcaaaggt ttcgacagtt tggaagagcc ggccgaattg gaagaagacc tgctcgatca
     1681 ggcttggggt ttggaagccg attcgcgcct gagttgtcag gcggttgttg ccggcgagga
     1741 tttgattgtg gaaatcccta aatacaccat caaccacgcg cgcgaagaac actgaaaaca
     1801 ggccgtctga agccggcacg cttcagacgg cattgttgcg cggataaggc gcaattaccg
     1861 aaaaacagac gtttgtgaag acggaacttt cgattctata ttgattttta tcggtttttt
     1921 gacggaacga cccgatacgg catctgaggc ggcttgcgtt tcttcctgcc cgcctgccgg
     1981 atttgcctgt ccttgcgcga aaccgaaaga gacggcggcg cggcggacaa ggtcgagata
     2041 gcgtccttca agctcggggc aggcggcgga cacgctttct gccgtaaccg tgaagccgcc
     2101 cgaccgactc ggaaaggcgt tccgcaatct gcatgcagat gcgccaacgc cgggcgcgca
     2161 acattgccgc atcgcaaccg acagccgctt tatccaaagc aatcagaccg cgcctgctcc
     2221 ggtagttaag gcaaaccgtt actctgtaga gggtatgttg cccgtccaat atttgataca
     2281 gtttgcgtat cagcagaatc aggcggtcaa actcctccat ctccgacagg gagtcgggat
     2341 ggtcggaagc ggtataaacc agttcggaca attttgcggc aaacatattg ttcatcaatc
     2401 ttccttatcg gttgaaaccc cgctcttcag ggcggtagaa tcagatttgt ttgggagggg
     2461 taactcctcc cggatcagga cgacacataa ggtggtgctt gatgtgccgt ccggcgagtt
     2521 gaaacattca gccatcctca aggggcggca gttttgccga aacatattct acacggcttc
     2581 aatgccggac gataaaagga aatttatatg aaatggaccg atacccggcg catcgccgaa
     2641 gaactctacg acctgcacgg cgaagccatc gatcccaaaa ccgtgcgctt tacccaactg
     2701 cgcgacctga ttatggcatt gcccgaattt gacgacgatc ccgcccgttg cggcgaacgc
     2761 atccttgaag ccgtgcagca ggcatggata gacgaggcgg aataagtttc aggaatgccg
     2821 tctgaaatgc ggcggtacgc ggttcgtgct tctgtttgca gcgggaatgg ttttaccagt
     2881 ctcctttttt cagcctgtcc agttggcggc ggtcgcgctt ggtcggtctg ccgtcgggat
     2941 gggcggaagt gatgcggctg aattggtcga gctgtttgca ctcttccctc aatgctgccg
     3001 ttttcgcgtc ttcttcatac agaagccgcg cctcgggtgc cgggcggcgt tggtggttca
     3061 aacctttaac cttgatttta tggggaaggg aattgagcgt caggtcgata atatcgccga
     3121 tgtctatggt tttactgttt ttgactttcg agccgtttac ttgaacccta cccagttcga
     3181 tatgcttttg cgcaagggaa cgggtcttga aaaaacgtgc cgcccaaagc catttgtcca
     3241 gccgcatggc ggaagaatcg tgcttgtctt tcatacgatt ttgtttgaaa taattgaatt
     3301 tgtttcgagt ttagcataag atacatcgcc ttataactag tatatatgca ctaatccgct
     3361 gttttccatg ctgtccgaac acaaaaagag ggtgtggaaa agccgttttg gacaataaat
     3421 taactgcgga atatgcacaa atagcgtatg atagcggcag aatctgttga tgagagcttc
     3481 attttatgaa acctgttttt ttggattttg aacaacccat agccgaactg accaacaaaa
     3541 tcgatgagct gcgtttcgtc caagacgagt ctgccgtcga tatttcggac gaaatacacc
     3601 gtttgcagaa aaaaagcaac gatttgacca aatcgatttt cagcaaactc acacccgctc
     3661 aaatttcaca ggtttcccgg catccgcagc gtccctatac tttggattac attgacgcgc
     3721 tgtttaccga ttttgaagag ctgcacggcg accgccactt tgccgacgat catgcgattg
     3781 tcggcggatt ggcgcgtttc aacggacaaa gcgttgtggt cgtcgggcat cagaaagggc
     3841 gcgacaccaa agaaaaaatc cgccgcaact tcggtatgcc ccgtcctgaa ggctaccgca
     3901 aagccctgcg cctgatgaaa acggcggaaa aattcggctt gcccgtcatg acctttatcg
     3961 atacgccggg cgcgtatccc ggcatcggcg cggaagaacg cgggcagtcg gaagccatcg
     4021 gcaaaaacct gtacgaactg acccgactgc gcgttcccgt tttgtgtacc gtcatcggcg
     4081 aaggcggttc gggcggtgcg ttggcggtcg ccgtaggcga ttacgtcaat atgctgcaat
     4141 attcgaccta ttccgttatt tcccccgaag gctgtgcttc gattttgtgg aaaaccgccg
     4201 aaaaggcggc tgatgcggca caggctttgg gtattactgc cgaccgcctg caaaagctgg
     4261 acttggtcga taccgtcatt aaagagcctt tgggtggtgc gcatagggat ttcgggcaaa
     4321 ccatgaaaaa cgtaaaagcc gttttggaaa aacaactgca cgaagcgcaa agcatcccgc
     4381 ttgccgattt gctttcgcgc cgtttcgacc gcattatggc ttacggcaaa ttttcggaac
     4441 aataattcgg gtagaacaag cagcaagcag tttgtctgaa actgcttgct ttttctttat
     4501 cgggatgaag ccgtgctgac tttagatgcg tttgagcaat gcttgaagga ttgttttcct
     4561 caaggtctga atggaaaaaa aacagcagtg gcattaagcg gcggcttgga ttccgtcgtt
     4621 ttgctgcatc tgctggtttg tgccgggaag cgggcaggat ttgtgccgga ggcattgcac
     4681 atccatcacg gcttgagtcc ccgagccgac gattgggcgg atttctgccg gaattattgc
     4741 gatatgctag gggtggggct ggaaacggtt aaggtctgcg tggaaaaaaa cggtttgggc
     4801 atcgaggcgg cggcaaggca aaagcgttat gccgagtttg ccgaaaaagg ctttgacgtt
     4861 ttggcgttgg cgcaccacag ggacgatcaa atcgaaacct ttatgctggc agtcgcgcgc
     4921 ggcggaggtt tgcgcgcttt ggcggctatg cccgccgtcc gccctttggg ggaaaacggc
     4981 attatctggc ggcctttgct gcctttttcg cgccaagaca tatgggatta tgcccgaaaa
     5041 cacggtttgc cgaatatcga ggatgaaagc aataccgata cggcttattt gcgaaaccgc
     5101 ttccggcacc gtattttgcc cgaactttcg gcgcagattc cccatttcgg gcggcatgtg
     5161 ctgaacaatg tccgcgcttt gcaggaagat ttggctttgt tggaagaggt cgtcgttcaa
     5221 gactgccgtt gggtttgcgg ggccggttat ttcgatacgg cgcggtggct gacgttttcc
     5281 ccgcgccgga aaacccatat tttgcggaat tttctaaagg aaaacggcat ccccgtgccg
     5341 aatcagaacg cacttgccga cattgcccgc gttttgacgg aggcaaaaac cggacgttgg
     5401 aacttgcaag gctttgaatt gcatcattat gcaggcaggc tgtttgtgtt tgcgtcggaa
     5461 cagttggcaa aaccggcttt tctgaaagat gggacgataa gcggaaatct caaaaaaata
     5521 ctgacagaac atagatttgt gttgaagcgg catccgttcg gacttcctga agccatgttg
     5581 gaacaggacg ggattttgag gacggtagcg gcatcggata cgttggctgt cggcggcatc
     5641 cataaaaatg tgaaaaaaat ccttcagggg aaacgggttc tgcctttcct gcgcccaatt
     5701 tggccgctcg ttgccgacag cggaaaccgt ccattggcgt tggcaaactg ttgtgcggat
     5761 tttcaaatct cggtttcaga cggcattttg cccgtacatc ctgactttcc cattttattt
     5821 tgataatatc gcaaacagat ttcggcggcg ttcagtcggg tattgtccgg ttgcatattt
     5881 ctaaaaggct tgtgaagtga aacacatcag ttcgaccaat aatgaacata tcagacacct
     5941 gcaccgcctg ttgtcgcaag gaaagttcag acggcagtat gcccaaaccg ttttggaggg
     6001 cgtgcacctg cttcaggttt tcctgcaatc cggcagaaag ccggtcgggg tatatattcc
     6061 cgaagctaaa atgccgtctg aagaagtcct taaattgaca gcggttttgc cggaagacgg
     6121 gattttttcc gtttcagacg gcatattgaa aaaaatcagc agcttgagtt gtgcagacga
     6181 tatacttaca ctgattgata tcccattggg cggaaccttg ccggacaaag gcgactgtgc
     6241 ggttttggac ggcgtgcaag atccgggcaa tgtcggcacc gtcttacgga gtgcggcggc
     6301 ggcaggggta ggtacggtcg ttttgggcag gggttgtgcg gacgcgtggt cgcccaaggt
     6361 attacgcgcg ggtatgggcg cgcatttctt gttggacatt tattcgcagg cggatttgga
     6421 aatatggttg gcgcactatg aagaccgtgt gtttgccacc gcgttgcgcg aggaaaagca
     6481 ggcggttttg tacggcgaag atttgtgcga accgacagct tgggtgttcg gcaacgaagg
     6541 tgcgggtgtc ggtaaagcag ttttagacag ggcggacaag tgtgtcagga taccgatgca
     6601 cgatgcaacc gagtctttaa atgtcgcgat ggcggcgaca atctgcctgt ttgaacagat
     6661 gcgccaacgg gcggcgtatt gaggaagaga aatgccgtct gaaaaaatct attacggcgt
     6721 attgattttc ttatgtatcg cttctatgct gctgtcaccg tttttttatg cgggtgcttt
     6781 gaagcccaag aaggcggcat tgcggaagga cgggcagtgg aaactcatct tatcgtccaa
     6841 tgccgtggcg gcggcggttt tgttttggat atggtggaaa tggttttgac agatattgct
     6901 caaaatcgtg ctaatggaat ccgaacaaat aaagaatttg gtaaaaaatt tgttaaatca
     6961 acggattaaa gttttgtgga aaacaaaaca gctctaagca aatagggcgt ttgtcggtaa
     7021 atacggaaga gttgcggcat tatcgggcat ctttaacaag tagtgtcggc ttggcaggca
     7081 atcggttttt atgggcagct tgcaaaatcg cggatataaa attgcgaatc ggttaaagtg
     7141 tggggacgct atgaaaaatt gcgaattttt ttatgatccg acaagggcaa tctatgatag
     7201 cggtgcagat tacttaacta gggaaaaata tagattagtc gtaatcgcaa atagcgcttg
     7261 ggggctattg ctgaatttac cttgttatta tgacgaggtt ttggaaaagc ggaaaatacc
     7321 gttcggcaaa caggaaattg atgacgatat ggacaaagtg tccgccctta agcggaagtt
     7381 taaagacatt tctgaaatca aagtagggga tggttgggaa tacccgttca attatgagca
     7441 aggaatgaaa gaattagatg aagttctatt gaaatacatt cctttttttg aagagaaaca
     7501 ataaaggagt ttaatatgcg cgtatctaaa atgattggaa gtatattgct tgttgcagcg
     7561 gttcagaccg tattttcggc aaatgtttac gagtgccgcc ataatggtaa aaccagttac
     7621 agccaaactc cgggaaaaca ttgtaccaac gcgggtttgg ggcgggatcg ggtgtacagt
     7681 tcggtcaggc ctgccgtgaa agacagggcg gaagacgcag gagttggcga ttattcggac
     7741 acggtgaggg acgaagccgt ccaaaatccg aaagggaatg cacagaaaga cggttcggat
     7801 gccggcatca gaccgcattg attgaaaccg aatcaaccat tacggtgttg aacaatagaa
     7861 tttgaacgat tagggaactc tgatgaaaca catccacatt atcggtatcg gcggcacgtt
     7921 tatgggcggg attgccgcca ttgccaaaga agccgggttc aaagtcagcg gttgcgacgc
     7981 gaagatgtat ccgccgatga gcacccagct cgaagccttg ggcataggcg tacacgaggg
     8041 cttcgatgcc gcgcagttgg aggaatttca agccgatatt tacgtcatcg gcaatgtcgc
     8101 caggcgcggg atggatgtgg tcgaggcgat tttgaaccgt gggctgcctt atatttccgg
     8161 cccgcaatgg ctggctgaaa acgtgctgca ccatcattgg gtactcggcg tggcagggac
     8221 gcacggcaaa acgaccaccg cgtccatgct cgcctgggtc ttggaatatg ccggactcgc
     8281 gccgggcttc ctcatcggcg gtgtaccgga aaatttcggc gtttccgccc gcctaccgca
     8341 aacgccgcgt caagacccga acagcaaatc gccgtttttc gtcatcgaag ccgacgaata
     8401 cgacaccgcc tttttcgaca aacgctccaa attcgtgcat tatcgcccgc gtaccgccgt
     8461 gttgaacaat ctggaattcg accacgccga catcttcgcc gacttgggcg cgatacagac
     8521 ccagttccac cacctcgtgc gcaccgtacc atccgaaggc ctcatcgtct gcaacggaca
     8581 gcagcaaagc ctgcaagata ctttggacaa aggctgctgg acgccggtgg aaaaattcgg
     8641 caccggacac ggctggcaga ttggtgaagt caatgccgac ggctcgttcg acgtattgct
     8701 tgacggcaaa aaagccggac acgtcgcatg ggatttgatg ggcggacaca accgcatgaa
     8761 cgcgctcgcc gtcatcgctg ccgcacgcca tgccggagtc gatgttcaga cggcctgcga
     8821 agccttgggt gcgtttaaaa acgtcaaacg ccgcatggaa atcaaaggca cggcaaacgg
     8881 catcaccgtt tacgacgatt tcgcccacca cccgaccgcc atcgaaacca cgattcaagg
     8941 tttgcgccaa cgtgtcggcg gcgcgcgcat cctcgccgtc ctcgagccgc gttccaacac
     9001 catgaaactc ggcacgatga agtccgccct gcccgcaagc ctcaaagaag ccgaccaagt
     9061 gttctgctac gccggcggcg cggactggga cgttgccgaa gccctcgcgc ctttgggctg
     9121 caggctgcgc gtcggtaaag atttcgatac cttcgttgcc gaaattgtga aaaacgcccg
     9181 aaccggcgac catattttgg tgatgagcaa cggcggtttc ggcggaatac acaccaaact
     9241 gctggacgct ttgagatagc ccgggcaatg ccgtctgaaa gccctttaga cggcattgcc
     9301 cggctgcgcg gcacaaaggc ggaaaaaccg tttgccccgt attttcaaac gcgttacact
     9361 tgccgccgct gttttcagcc atttgattac ccgcaaccgc cgtcattgcg ccggcggttt
     9421 gcctgtcagc gtcattgcgc cgctgtaaat acgaaagaac acattatgac cgtatccccc
     9481 gtcgccttgc gccgtaagac cgagtgcaag cctcatccca ccgcgcgcta ttggaaaaaa
     9541 tgcgatgtcg aggcactttt cggactgccg tttttagaac ttgtctatca ggcggcagaa
     9601 gtccaccgcc aaaatttcaa cccgcgcgaa atccagcttt ccacgctgtt gtccatcaaa
     9661 accggcggtt gtcccgaaga ttgcgcctac tgtccgcaat cagcgcacca caacaccaat
     9721 ttgggcaaag agcagatgat ggatgtggat gaaatcgtcg aaaaagccaa aatcgccaaa
     9781 tcgcgcggcg caagccggtt ttgtatgggc gcggcatggc gcggccccaa acccaaagac
     9841 gtggagacgg tttccgcaat catcaaagcc gtcaagggct taggtatgga aacctgcggc
     9901 acgttcggta tgctcgaaga aggtatggcg gaagacttga aagaggcggg cttggattat
     9961 tacaaccaca acctcgacac cgaccccgac cgctacaacg acatcatcca cacacgccgg
    10021 cacgaagacc gtatggatac cttgggcaaa gtccgcaacg ccggtttgaa agtctgctgc
    10081 ggcggcatcg tcgggatgaa cgaaacccgc gccgaacgcg ccgggctgat tgccagcctc
    10141 gccaacctcg acccgcagcc cgaaagcgtg ccgattaacc ggttggtcaa agtggaaggc
    10201 acgccgcttg ccgatgccga agatttggat tggaccgaat ttgtccgtac ggtttccgta
    10261 gcgcggatta cgatgccgca aagttatgtc cgcctctcgg cagggcgcag caatatgcct
    10321 gaagcgatgc aggcgatgtg cttcatggcg ggtgcgaact cgatttttta cggcgacaag
    10381 ctgttgacca caggcaatcc tgatgaggac ggcgatagaa tcctgatgga aaagcttaac
    10441 ctgtatccct tgcagtttga gccggaaggc gaggtcgccg aagtggaaaa agcctccggg
    10501 attaaagcgg attattgacg attgaaaaat gccgtctgaa acttgaatgc cgtctgaaac
    10561 ctgaaaaaag gctttcagac ggcatttgtt cggacggaat ttccaatatc tttttaccgg
    10621 cgcgtgatgt cgccgccggg cgagacatcc agtccgttcc cgttggggaa gtcgctatgg
    10681 ttcagataaa taatccgctc aataacagtt ttttcagggt cgattttggg gattttatcg
    10741 ccgacttgag tgatggggat aatgttgccg gaaacgaacc gcccctgttt gtcggtgata
    10801 attttgaaaa tcggcgcaat gccgctgatg ccgtaggtgg caaagttgcc gccgctgtaa
    10861 gagatgaagc ggtcgtggta aagttcgacg gcgcgagtaa cgtgcgcccc ctgcccgaat
    10921 acgacatccg cgccggaatc gacggcgagc cgcgcaaact cgacgacgtt gcccctgttt
    10981 tccccataga agatttcggt atcgaacggc aggtgttcag cctgtttccc ttccgcgccg
    11041 ccgtggaaca tcacaatgac gatgtcggtt ttttgtttgg ttttccgaat cagttttttg
    11101 aatttggcgt aatcgttcag tttgacggcg gcaaggttgg gggcgaagga gacgaagccg
    11161 tatctcacgc cgtttttctt caggatggcg gtttcaaacc tgttttcgat gcccgaatat
    11221 ttgatgttca attcgtcaag gttgcctgcc gttgccgtga tgccttgcgc gccgaagccg
    11281 ttgctgtggt tgtttgcgag gctgaggtag tcgaatcccg cgtcggcaag gtattgcccg
    11341 tatgcggagg gcgttcggaa tgcatagcat attttggtgg tttgcacatt ttttcggcgt
    11401 accgccttcg tcaaacagcg taccttatgc cgccgacccc gatgatggaa acggtatctg
    11461 ctgcctttcc gtcaggcgta tcctgtccgt ccgcatcgcc ggtgtttgaa acgggcgcgg
    11521 ggtttgaacc gtcaatcggc agcacggggg attccgttcc ggcaggatgc gccgaggctt
    11581 gaccccggcg gttttcggcg cggaaacagc ataaggggcg gcaggcggct cttttacata
    11641 ctcgagcgcg gtaaagccgc tgccgataac gagggccaaa attgcggaaa ataccgccat
    11701 cgcccagata aatttgttaa taatcatacc tttactgttc agacggcatt tgccgcacgt
    11761 tttggggctt atctctcgat ttgcgatacg tcgcgcaccg cgcctttgtc ggcggaagtc
    11821 gccatcgcgc cgtaagccct taatgcggcg gagacgtagc ggtcgcggtt ttccggtttc
    11881 cacgctttgc tgccgcgagc ttccatttcg gcacggcgtg cggcaagctc ttcatcggaa
    11941 atgacaaggc ggatgctgcg tttggggatg tcgatttcaa tcgtatcgcc ttcgtgtacc
    12001 aaaccgattg cgccgccttc cgccgcttcg ggcgaggcgt gtccgatgga caaacctgat
    12061 gtgccgccgg agaagcgtcc gtcggttaag agggcgcagg ctttgcccaa gcctttggat
    12121 ttcaggtagg aggtcgggta tagcatttcc tgcatacccg ggccgccttt cgggccttcg
    12181 tagcggatga tgacgatgtt gccggcgacg atttggttgc ccaaaatatc ttcgacggcg
    12241 gcttcctggc tttcaaacac gcgcgcgcgg ccggtgaatt tgaggatgct ctcgtccacg
    12301 cctgcggttt ttaccacgca gccgcgctcg gcgatgttgc cgaataagac cgccaaaccg
    12361 ccgtcttgcg agtaggcgtg tgccacgtcg cggatacagc ctttttcgcg gtcaaggtcg
    12421 agggttttcc aaatacggtt ttgcgagaac gcctgagtgg tgcgcacgcc gccgggagcc
    12481 gctttgaaac gttcgatggc gcgggtgttt tcgggattgg tgacgtccca tttttcaatc
    12541 gcgtctttca gtgtcggcgc gtggatcgtg tacacgtcgg tgtgcagttt gcccgctttg
    12601 tcaagttctt tcagaatggc gaagatgccg ccggcgcggt gcacgtcttc catatagtag
    12661 tcgtggttgt tgggcgcggt tttgcagatg cagggcacga cgcggcttaa gcggtcgatg
    12721 tctgccattt tgaaatccac gcccgcttcg ttggcgacgg cgagcaagtg caggatggta
    12781 ttggtgctgc ctcccatcgc aatatccatc gtcatggcgt tttcaaacgc ttttttggtg
    12841 gcaatgctgc gcggcagcac ggtttcgtcg tcttgctcgt aatagcgttt ggtgatttcg
    12901 acaatcatac gccctgcttc gaggaacaat tctttgcggc ctgcgtgggt cgccaaatac
    12961 gaaccgttgc cgggcaggga cagaccgagc gcttcggtca ggcagttcat cgagtttgcc
    13021 gtaaacatac ccgaacacga gccgcaggtc gggcaggcgt tttgttcgac ttcttcgact
    13081 tgccggttgc tgacattgtc gtccgccgat tcgatcatgg cgtcaatcaa gtccaaacgg
    13141 cgttcgggtt ggatgtttgc cacgccgatg accttgcccg cttccatcgg gccgccggag
    13201 acgaagatgg tggggatgtt caggcgcatg gcggcaatca gcattcccgg ggtgattttg
    13261 tcgcagttgg aaatgcacac cagcgcgtcg gcgcagtggg cgttgaccat atattcgatg
    13321 gagtcggcaa tcaaatcgcg gctgggcagg gagtacaaca taccgctgtg ccccatggcg
    13381 atgccgtcgt cgatggcgat ggtgttgaat tctttggcaa ttgcacccgc tttttcaatc
    13441 tcgcgggcaa ccagctggcc catattgtgc aggtggacat ggccgggcac gaattgggtg
    13501 aaggagttgg caacggcgat gatgggcttg ccgaagtcgg tttccatcac gccggtggcg
    13561 cgccacaatg cgcgcgcgcc cgccatattg cggccgtggg tggaggtttt ggagcggtat
    13621 tctggcatag tgtgtttcct tgtgcctata ccgtctgaaa ggcagggctg tttcagacgg
    13681 catcgggtac ggttttttag agtgggaaaa ggggggattt tataccaagt atcggaattt
    13741 tgcggaatgg aaacgtgtgc ggcaaaaaag aacatccccg caggaatgcg ggggacgggt
    13801 tcaggcgcgc gcaatcggga cggctttgga tgcgtcccaa aaatcgacgg gcgcatccaa
    13861 cacgggtttg acgatgcccg tccgtatggc gaaatcgccg aaaccttcgt cggtatcgcg
    13921 tcccgccgcc catttgccga tcaggtcttc caattcggaa aggatttccg gcagggtgat
    13981 gttttctttg tagaggcggg ggatgcgtac gccttcgcgg tcgccgccga tgtggaggtt
    14041 gtagcgtccc acggctttgc cgaccagtcc gatttccgcc aacatcgccc gtccgcagcc
    14101 gttcgggcag ccggtaatgc gggtaacgat gtagtcgtcc gacgcgccgt gttccgccat
    14161 aatcttatcc agctcgccga tgaagtccgg caacacgcgt tcggcttccg ccattgccag
    14221 cgggcaggtc gggaaggaag cgcacgacat cgcattttcg cgcagcttgc tgacaccgtc
    14281 gcgtatcagc ccgtatgagc gggcgagctg ttcgatccgg gctttgcctc cttcgggcac
    14341 atttgccacg atgaggtttt ggttggcggt gatgcggaag ccgcctttgt ggattttggc
    14401 gatttccaac acgccggtca gaagctgttt cccgccttcg tcaaccaaac gcccgctttc
    14461 gatgaaaagg gtcaaatgcc agttgccgtc tatgcctttc acccggccga tgcggtcgcc
    14521 gcgcccggta aacttgaacg gccgtatggg tttgaacggc atacccatac ggcgttcgac
    14581 ttccgtgcgg aagttgtcca agcccatatt ttgaatggtg tagcgggtgc ggtcgttttt
    14641 gcggtggctg cggttgccga agttgcgctg cgtggttacc accgcttcgg cggccttcag
    14701 tgcgtgttcc ggaggcacga aacccagttc cagtgaaatg ttcggatagg ttttggtgtt
    14761 gccgttgccg tgttccgtcg aaagcccgcc gcccgccaaa acattgaagc cggcaagctg
    14821 tccgttgccg tctgaaacgg cgacgaaatc caaatcgtta ccgtagcagt ccacatcgtt
    14881 caagggcggg atgacgactg cggttttgaa ttttcgcggc agataggttt tgcccaaaat
    14941 cggctcgcct tcttgaagga agtcgtcgga actttgaact tttttgccgt ccacccacat
    15001 atccagataa ccgcgcgtgc gcggcagcag gtgttcggaa atctttttcg cgtattcgta
    15061 agcctgccgg tgcagctcgg actcgatcgg gttggaggtg caaagcacat tgcggttcat
    15121 atccgccgcc gtagcgatgg aatccaaacc caggctgtgc aagaggcggg gtatcgtctg
    15181 caacttggtt ttcggcacgc cgtgaaattg gaaggtttgc cggttggtta gccggatgga
    15241 gcggtaatga ctcttttccc gggcgaattt gtccagttct atccactggg acggtttgat
    15301 gatcccgccg gcagccggca gcgcaaaagc ataaatttca agggctcgag ttttgcctcg
    15361 gtgcggatgt cgcggccatc ctgctcatac atactgtgga agcggatgag ttggaagttg
    15421 tcgcctttga agccgcccgt gagcgggtct ttcaaatcgt ccaaaatcgt gccgcgtaaa
    15481 aaattgcttt cggttttcag acgttcgttg tcggatagcg gcttttcttg ccacgccaaa
    15541 ccttttgtct tggcctgtac ggtcattttg tgttcctccc gattatattt aatcaataaa
    15601 catcacgctg atagcgtttt tcttcgcgca gcatatccaa atatccttct gcgccgtctt
    15661 cgtccgaatg ccctgccccg ataatcacat ccagcaaggc ggcttccact tcttttgcca
    15721 tttttgccgc atcgccgcac acatagatat gcgcgccttc ctgcagccat tgccaaagtc
    15781 cttccgcctg ttcgcggatt ttgtcctgca catagatttt ttcttcctga tcgcgcgacc
    15841 aggcgaagtc atatctgtgc aggaagccgt ctttggcaaa ctgctgccat tcggtctgat
    15901 agaggaagtc ggcggcaaaa tgcggattgc cgaaaatcag ccagtttctg ccttccgcat
    15961 tttctgcggc acgttgttgg acgaaggcgc ggaacggtgc gacgccggta ccggagccga
    16021 tcatcacaat cggcttgcgg ctgtcttcgg gcagcctgaa gccgtcgttg cgttccgcaa
    16081 acacgcgcac cgtgccgtcc tcttccagcc ggtcggcaaa gaaacccgat gcgccgcccg
    16141 acctggcgcg cctttcgtgt tcgaaacgca ctgcgccgac ggtcaggtgc acttcgtccc
    16201 ccgcttccgc ctgcgacgag gaaatcgaat acaggcgcgg cgcaagcggg cgcagcaggc
    16261 cggcgaattg ttccgccgtc agttttgccg ggaagcggtg cagcacaccg gcaatcggcg
    16321 tgctttgcac aaagccttgc aaaacggcgt tgtcggcagc aatacggtcg agttcgtcat
    16381 catcggcgaa cgtggcatag cctttgacaa aggcgggggt gttttgcgtg agttcgaaat
    16441 gggataacag tgcggaggca accggcaggg tttttccgcc cgcctgtatt tccgttgccg
    16501 gatcgatgcc gagcaggtct aggatttccc cgaccagtgc cggatcgttg tcaaaccaaa
    16561 cgccgagcgc gtcgcccggg aggtagtgca aatccgaacc gctcaaatcg atttcgatgt
    16621 ggcgcacgtc tttatcggat tggcgggcgg tgattttctg attggccagc agggcggcgg
    16681 gaaaggggtc tgccttgcag tacctgccct ccgatgccgt ctgaaggccg gcggggggac
    16741 gttgcctgcg gcgcgggcgt tgcccggttt tttgcggctt cttcttttaa gcgcgcgaca
    16801 atgcggcctg tccatccgtc tgcggcggcg gcaaagtcca aatccgcatc aacgcgttcg
    16861 agcaggcgtt ttgcgcccaa ttcttcaaaa cgtttgtcga aatctttgcc cgcccggcag
    16921 aaattcggat aggagctgtc gcccaaaccc agtacggcaa attggagttt gtccaatttc
    16981 ggggcttttt tgccgttcag cagtttgtgc ggcacgacgg cttcttccgg cggttcgcct
    17041 tcgccctggg tggacgtaac cagcagcagg cggcgttcgc cggcgatgtt tttcgccttg
    17101 tagcctttca gttcggcgcg cctgacttgg atgccggcgg ctttcaggct gtccgccgct
    17161 ttgtcggcaa cggatttcgc attgccggtt tgcgaggcgg aaaggacggt tacggaaaaa
    17221 ggttctgccg ccggcaatgc cgtctgaagc gcgggcagcc ctgcggatgc cccgtttcct
    17281 gcttttgccc aagcgtagcc ggacagcccc gacacaggag ctgcgtgatt tcgggcggca
    17341 taggcggtaa tggcggattt gtgttctgca tatcgtgttc actcataaaa tcatacctgc
    17401 cgcaacagta ccgtatgtcg cttcgtctat caggataaac gaaccggcgg cggtgttttc
    17461 cgcataaggc gttgccgtaa cgggtttttg aaggttgatg cggactttgg cgatgtcgtt
    17521 catctttaag gattccgcgc cggtctcttg ttccagcgtg cggacatcca aaacgctttc
    17581 aatttccccg acttttgccg acacggtttg cgtgccgtgc ttgagcaggt atttgcgcgc
    17641 ggtgttgagc gggcgttcgt caaaccagca aagcgtggct tccggatgtt tttgcggggc
    17701 gagcgggaaa tttttatcga caaaaagatc gccgcgcgaa acatcgatgt cgcggtccag
    17761 ccggatggtt gccgcttcgc cggcaaaagc ctgcgccact tcccctttcg gcgcgatgat
    17821 ttcggacact tcggcggtca gcccgttcgg ttcgatgcgg acggtttgcc cgacggtgac
    17881 cgaaccgcgt tcgatgcgcc cctgatagcc tcggaaaccg tcggccttgt cggcatcttg
    17941 gcggacgacc agttggacgg ggaaataaaa accggcatcg cgccaccttc ctgaaatttc
    18001 cgccatatcg cccaaagacg gcggcagcaa ggcttccagc ctttcacgca gcaggcgggc
    18061 gaggacgggc gcgctgccgg agctggaaac ggcaatctga acagggtcgc ggtcgataac
    18121 cgacgggaag atgaagctgc aatggtcacg gtcgcccacc acgttgaccg gcttttggca
    18181 gctttcggca agatggaaaa cgcgccggtt gagggcttgg tcgctgcttg ccgcaatgat
    18241 gaggaaaacc gtgcggatgt gttcggcacg aaattctttg gcaagccaca ggattttgtt
    18301 ttccgccgcc aacgcggaga gttcggcatt caggtgtttt gcggcaaccc cgacctctgc
    18361 gcccgccttc ggcagcaggc tgattttgcg tgcggcgacc gcgccgccgc ctacgaccaa
    18421 tacggggcgg ccggagaggt tggcgaaaat gggaaaataa ttcactggct gactcctttg
    18481 ctgtttgccc gcaccttgtt tccgatacgg tgcgtcgcgg catttttgtc ggaatgcggg
    18541 tcattttaaa taataaaaag gcatttgtta gaagctgaaa gctatatggg gggcggctgc
    18601 ggatgcggcg gttttccgtt ttatagcgga ttaacaaaaa ccggtacggc gttgccccgc
    18661 cccggctcaa agggaacggt tccctaaggc gcccaaacac cgggcgaacc ggttccgtac
    18721 cattcgtact gcctgcggcc cgccgccttg tcctgatttt tgttaatccg ctatataacg
    18781 gttttggacg gaaaaacggc ctgaagccgt ttcgggcatt cagaccgttt gtgcggtggg
    18841 gaaaatgccg tcagaaaggc agaaaagggc ttcagacggc atgatgtcgg gtttcaggac
    18901 aggagcagga tggcggctgc ggcaagcgcg gcaaccgata atgcggcggc aagcgcggct
    18961 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
    19021 cggacgaggt ttttcttttt gaatatgcgg tatgcggcga cggcggcgat gtggactgcg
    19081 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgtataag gctgcccgta
    19141 tgttcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
    19201 aaaagccccg tgccgacttg aaatgagacg gcggccaaaa gcgcaacgac catcagtgcg
    19261 cccaaggggt tgtgtccggg ctggatatgt tcgggaatgc cgtttttcag atagccgcgt
    19321 atacctgccc aacctcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
    19381 cagcagaggc ggaatacgag caggaaaagg acgagcagcc cgacgcgcgt gtgccattgc
    19441 agcatatcgc cgccggcttt tgcgctatac cacataaagg gcagggatgc ggcaagcagc
    19501 cagtggaaaa ggcgggtggg gaagtcccag actttggttt tgtttttcat aatcggtttc
    19561 cggcggtaga atcggtttgt tttcgagcct taattttaac cgattggagg ggaaatgttt
    19621 cccgttttcc atctttcagg cgaaagccgc cgccggatgc ttcagacggc attgcgtttt
    19681 ccccatgttt tcaaagcccg tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc
    19741 gtagtcggcg gatcggcagg gatgagcggc gcgcccgtat tggcggcatc ggcggcaatg
    19801 tatctcggct gcggtaaggt gcgggcaggt ttcaatcagg atacgctgcc ttttgccgtc
    19861 attgccggtt ttcccgagat tatgttggac acggcagacg gtttgaccaa gcgtcaaggc
    19921 ataaacgctt ggacggcggg ttgcggcttg ggtacagatg cggcggcagt ggaaacggtg
    19981 gcggcagtgt tggcgcggaa tcgagacgaa gccgtcgttt tggatgcgga cgcgctgaac
    20041 atattatcaa ccgatgccga aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta
    20101 acgccgcacc ccgccgaagc cgcgcgcctg cttggaacga cagttgcaca ggttcaggcg
    20161 gatcggacgg cggcagtaag gaagataggg gcaattctcg gtgcaaccgt ggttttaaag
    20221 gggcacaaaa cattggttgc cgcgtccgat acggaaatct atgtcaacga aagcggcaat
    20281 gcgggattgg caacggcggg cagtggcgac gtattgggcg gcatcatcgg cagtctgctc
    20341 gcacagggcg tgccggtttt tgaagccgcc tgcgcgggcg cgtggctgca cggcgcggcg
    20401 gcggatgtta taaaggaatc ggcaggcatt gcggctgggc tgtcggcagg ggaaatcgcc
    20461 ccggcggcaa ggtggctgcg caactggata actgaaagta tgtaagaaga tgccgtctga
    20521 aaggcaaggg cttcagacgg catcttcatt ttccaaatac tgtccggtaa atcgtggtat
    20581 aatacgcagt tatctcttac agtttttgaa aaacattaat tatgaaacaa atccgcaaca
    20641 tcgccatcat cgcacacgtc gaccacggca aaaccacatt ggtcgaccaa ctgctgcgcc
    20701 aatccggcac attccgcgcc aaccagcagg ttgacgagcg cgtgatggac agcaacgacc
    20761 ttgaaaaaga acgcggcatc accatcctcg ccaaaaacac cgccatcgat tacgaaggct
    20821 gccacatcaa tatcgtcgac acgccgggac acgccgactt cggcggcgaa gtggagcgcg
    20881 ttttggggat ggtggattgc gtcgtcttgt tggtggacgc acaggaaggt ccgatgccgc
    20941 aaacccgttt cgtgaccaaa aaagccttgg ctttggggct gaaaccgatt gtcgtcatca
    21001 acaaaatcga caaaccgtcc gcccgtccga gctgggttat cgaccagact ttcgagttgt
    21061 tcgacaactt gggtgcgacc gacgagcagt tggatttccc gattgtttac gcttcaggtt
    21121 tgagcggctt tgccaagctg gaagaaaccg acgagagcag cgatatgcgc ccgctgttcg
    21181 acaccatcct aaaatacacg cctgcaccga gcggcagcgc ggacgagccg ctgcaactgc
    21241 aaatttccca actcgactac gacaactaca ccggccgcct cggtatcggt cgtatcttga
    21301 acggacgcat caaacccggt caaaccgttg ccgtgatgaa ccacgagcag caaatcgccc
    21361 aaggccgcat caaccagctt ttgggtttca aaggcttgga acgcgtgccg cttgaagaag
    21421 ccgaagccgg cgacatcgtg attatttccg gtatcgaaga catcggcatc ggcgtaacca
    21481 tcaccgacaa agacaacccc aaaggcctgc cgatgttgag agtggacgaa ccgacgctga
    21541 cgatggactt tatggtaaac accagcccgc tcgcaggtac agaaggcaaa ttcgtgacca
    21601 gccgccaaat ccgcgaccgc ctgcaaaaag aattgctgac caacgttgcc ctgcgcgtgg
    21661 aagacaccgc cgatgccgac gtgttccgcg tatccgggcg cggcgaactg cacctgacga
    21721 ttttgctgga aaatatgcgc cgcgaaggct acgaactcgc cgtcggcaag ccgcgcgtcg
    21781 tgtaccgaga catcgacggt caaaaatgcg aaccttatga aaacctgact gtggacgtac
    21841 ccgacgacaa ccaaggcgcg gtaatggaag aactcggccg ccgccgtggc gaactgacca
    21901 atatggaaag cgacggcaac ggacgcaccc gcctcgaata ccatattcca gcgcgcggct
    21961 tgatcggttt ccaaggcgaa ttcatgaccc tgacgcgcgg cgtcgggctg atgagccacg
    22021 tgttcgacga ctacgcgccc gtcaaacccg atatgcccgg ccgccacaac ggcgtactgg
    22081 tgtcccaaga gcagggcgag gcggttgctt acgccttgtg gaatcttgaa gaccgcggcc
    22141 gtatgttcgt atcgcccaac gacaaaatct acgaaggtat gattatcggc atccacagcc
    22201 gcgacaacga tttggtggtc aacccgctca aaggcaaaaa actcaccaat atccgtgcca
    22261 gcggtaccga cgaagcggtg cgcctgacca cgccgatcaa actgacgctg gaaggcgcgg
    22321 tcgagtttat cgacgatgac gagctggtgg aaatcacgcc gcaatccatc cgcctgcgca
    22381 agcgttacct gagcgaattg gaacgccgcc gtcattttaa aaagctggat taatgtttaa
    22441 atgatacgcg atgccgtctg aaaaatttca gacggtattt tttattcgga cgggctttgc
    22501 ggcttcttga agctgtttca gacggcgttt ttcctatcca atcaagaaac tgccgccatt
    22561 tttccagcgg catatcgccc cgtcgcgttt cggtatcggg ttcggtttcc cggcagcatg
    22621 tcgaacaata tcgtttgaag ggatacgcct tttgagtcct tacaggctta ggaagaggat
    22681 aaaacatacc gcaaaacata ccataaaaaa acggtaacgg atagtaatca atggtaacgt
    22741 agggtaatag gttgtggctg attttacgca ataaaatcaa tacgtaagta acaaaaaatc
    22801 ccaaactgta aaagtttggg atttgtactt ggcagagagg aagggattcg aaccctcgat
    22861 acgctattca cgtatacacg ctttccaggc gtgcgactta aaccactcat ccacctctct
    22921 aatggcggaa attatcctaa tcgggataat ttgttatttg gtgcccggga gaagactcga
    22981 acttccacac ccgtgaggat accagcacct gaagctggcg cgtctaccaa ttccgccacc
    23041 cgggctttgc tttatttgct gttttgcggt acagtgtcct gccgcaaaga agtcgctatt
    23101 atatagttca taaagagaat gtcaacagtc aaaatgaata aaaatattaa atctttaaat
    23161 ttacgggaaa aagacccgtt tttaagtcgt gaaaaacagc gttatgaaca tcctttgccc
    23221 agtcgggaat ggataatcga attgttggag cgcaaaggtg tgccttcaaa aatcgaatcg
    23281 cttgcacgcg agctgtcgat tacggaagac gagtatgtct tttttgaacg ccgtctgaag
    23341 gcgatggcgc gggacggtca ggttttaatc aaccgccgag gcgcagtttg cgcggcggac
    23401 aagctggatt tggtcaaatg ccgcgtcgag gcgcataagg acggtttcgg cttcgccgtg
    23461 ccgctcatgc cgatggacga aggggatttc gttttatacg aacgccagat gcgtggtgtc
    23521 atgcacggcg acaccgttac cgtccgtcct gcgggtatcg accgcagggg ccgccgcgaa
    23581 gggacggttc tggatattgt cgaacgcgcg caaagcaaag ttgtcggccg tttctatatg
    23641 gataggggcg tggcgatttt ggagccggaa gacaagcgtc tgaaccaaag catcgtgttg
    23701 gaaccggacg gcgtggcgcg tttcaaaccc gaatccggtc aggttatcgt cggcaaaatt
    23761 gaggtttatc ccgagcaaaa ccggcctgca gtggcaaaaa tcattgaagt tttgggcgat
    23821 tatgccgaca gcgggatgga aatcgaaatt gccgtgcgca agcatcattt gccgcaccga
    23881 ttcagtgaag cgtgtgccaa atccgcgaaa aaaattcccg accatgtacg caaaagcgat
    23941 ttgaaaggcc gcgtcgattt gtgcgacctt cctttggtaa cgatagacgg cgaaacggcg
    24001 cgcgatttcg acgacgcggt gtttgccgaa aaagtcggac gcaattaccg tctggtcgtg
    24061 gcgattgcgg atgtcagcca ttatgtccgc cctgacgatg cgattgatgc agatgctcaa
    24121 gaacgcagta ccagcgtgta tttcccgcgc cgtatgattc cgatgctgcc ggaaaacctg
    24181 tccaacggca tctgctcgct caatcccgat gtcgagcgtt tgtgtatggt gtgcgatatg
    24241 gtcgttacct atgcgggcaa tatcaaagaa taccgcttct atcccgccgt gatgcgctct
    24301 catgcccgcc tgacctacaa ccaagtttgg aaatggcttt cagacggcat cgggaatccg
    24361 cacaaagccc aaatcgacac gctttacaag ctgtttaaga ttttgcagaa aaaacgtctg
    24421 gcgcgcggcg cggtcgagtt tgaaagcgtc gaaacgcaaa tgcttttcga cggcaacggc
    24481 aaaatcgaaa aaatcgtgcc cgttgtccgc aacgatgccc ataagctgat tgaagaatgt
    24541 atgttggcgg cgaatgtttg cgcggcggat tttctgttga aaaacaagca taccgcattg
    24601 ttccgcaacc atttgggccc cacgtccgaa aaactcgcca ccctgcgcga gcagctcggt
    24661 ctgttggggc ttcaacttgg cggcggcgac aacccgtcgc cgaaagacta tgccgcgctt
    24721 gccgaacaat tcaaaggcag gccggatgcc gaattgctgc aagtcatgat gttgcgctcc
    24781 atgcagcagg cggtttacga accgcattgc gaagggcatt tcggtttggc ttatgaagca
    24841 tacgcccact ttacctcgcc catccgccgc tatcccgacc tgaccgtcca ccgtgccatc
    24901 aaagccgtat tgaaccggaa aacctacacg ccaaacaaaa gctggcaggc tttgggcgtg
    24961 catacttcgt tttgcgaacg ccgtgccgac gatgctggcc gcgatgtgga aaactggctg
    25021 aaaacttatt atatgcgcga taaggtcggt gaaatatttg aaggcaaaat ctccggggtg
    25081 gcaaattttg gaatatttgt cactttggac gatatccata tcgacggtct ggtacatatc
    25141 agcgatttgg gcgaagatta tttcaacttc cgccccgaaa tcatggcaat cgaaggcgaa
    25201 cgcagcggca tccgtttcaa tatgggggac agggttgccg tccgggtcgc gcgtgccgat
    25261 ttggatgatg gaaaaatcga ctttgtccta attgccggag gaagcggcag gcggcggaag
    25321 gtcaaattat ccgcatctgc caaaccggca ggggcggcgg ggaaagggaa atcgaaaacc
    25381 accgccgaga aaaaaacagc ccgatgcggc aaagtaaggg gaaggggcgt gcctgccgtt
    25441 gccgaatcgg ggaaaaaggc aaagaaaccg gttccgatta a
//
LOCUS       DAWTCI010000020        25372 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWTCI010000020 DAWTCI010000000
VERSION     DAWTCI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25372
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.020"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..173
                     /locus_tag="WM481_000846"
     CDS             <1..173
                     /locus_tag="WM481_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HFA9080201.1"
                     /translation="EPLPPSALSESEANRDLGWMLHDIDFDHGNTPHFFRTQMKDGLI
                     DVPPFYAEEVKA"
     gene            170..1036
                     /gene="cas8c"
                     /locus_tag="WM481_000847"
     CDS             170..1036
                     /gene="cas8c"
                     /locus_tag="WM481_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9080202.1"
                     /translation="MILASLVRYYRRLATETDETGNPKVPSYGFSEEKIGWILVLDKE
                     GRLKTVVPNLTADKKPQPKLMSVPRPEKRTSGIKPNFLWDKTAYALGVEANKNKAEAK
                     EKPFTPSEKTFEAFKQYHLDLLQNSEDEGLQALCRFLQNWQPAHFAAENLPAEMLDSN
                     TAFSLEKPTALIHKREAAQTLWAGCLKSDEALESLYLISGDTAPIARLHPAIKGVFGG
                     QSSGGSIISFNKEAFSSFGKEQGANAPVSEQSAFAYTTALNYLLRRENNHCLTIGDAS
                     TVFWAEADDIVD"
     gene            1024..1128
                     /locus_tag="WM481_000848"
                     /pseudo
     CDS             1024..1128
                     /locus_tag="WM481_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1121..1528
                     /locus_tag="WM481_000849"
     CDS             1121..1528
                     /locus_tag="WM481_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HFA9080203.1"
                     /translation="MHYNATAQAAEGFFAQVFTPSDDEQESAKIFNVLEQIGKGRPLQ
                     EIAPELSPNTRFYILGLAPNAARISVRFWLDTTFGQLAENLAHHWQDLALEPCAWKTP
                     PSIWRLLLQTAVLGKSENIQRLGNRCLPETQNP"
     gene            1476..2236
                     /gene="cas7c"
                     /locus_tag="WM481_000850"
                     /pseudo
     CDS             1476..2236
                     /gene="cas7c"
                     /locus_tag="WM481_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
     gene            2263..2631
                     /locus_tag="WM481_000851"
     CDS             2263..2631
                     /locus_tag="WM481_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080204.1"
                     /translation="MKYWLVGASWGGQGHQDQFFVENGYWVLEWESSQQPDQFAKGEK
                     IQVGDRIAIKRMKGQGSSEIKILHIGIVKGVISETNKIICVVDWIVKNLDRNVESRGC
                     FKSIHDPYDKDEWIEKIFCL"
     gene            2638..3288
                     /gene="cas4"
                     /locus_tag="WM481_000852"
     CDS             2638..3288
                     /gene="cas4"
                     /locus_tag="WM481_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFA9080205.1"
                     /translation="MTALLTETQRENQDTRLIPLSALQHYAFCPRQCALIHNEQAWAE
                     NYLTAQGKALHERVDSDEPETRKGVRFERTVHVSVEKPGISGILDLVEADTKTGRLKP
                     VEYKRGKPKPDPGDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRAQ
                     TLATIAAVCKLLNSGQTPPPDYGKRCKACSLAEICQPELLGKRDRSVGYVKGLFGE"
     gene            3404..3763
                     /locus_tag="WM481_000853"
     CDS             3404..3763
                     /locus_tag="WM481_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080206.1"
                     /translation="MTRNIEHQFSNLSDVGEKLELENPTVENVVDILVDIGHDDRVYT
                     FHDDFLGLKSGLPQDLLSKHIDELEEGDFADRYSDEIDKILDNANIIFYHLERELSED
                     DLEEIREERERLGLEDD"
     gene            3906..4529
                     /locus_tag="WM481_000854"
     CDS             3906..4529
                     /locus_tag="WM481_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080207.1"
                     /translation="MTQKNDFKNANFYGNTQIVNGDVHNVHTQNTPNSINENEIATYT
                     PEPIWRSPITMAWLTWASFLISLAGLLPIYNCIVKPILYLVNGKTMIQSEINNTYIFI
                     LIAIAVLFLITITLRRITKYQTRYPLVFNYAISGMGHRLSLEKISISQCPQCGGEMKY
                     YNKPIEWENIRYSDGSKKRRVTRTTPALECKRNREHWYKVDPAEDKI"
     gene            4589..5053
                     /gene="cas1"
                     /locus_tag="WM481_000855"
     CDS             4589..5053
                     /gene="cas1"
                     /locus_tag="WM481_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HFA9080208.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQERKKVAQLPVHSIGHI
                     FCFGNVLVSPFLLGFCGENNVNLAFFTENGCYLGRLQGRQNGNVLLRRAQYRVSEQSP
                     MPIACNIIAAKIRAIRKRARACVLSGRYTFRRKNRASAAYWIWWKWIQKQAV"
     gene            4957..5238
                     /locus_tag="WM481_000856"
     CDS             4957..5238
                     /locus_tag="WM481_000856"
                     /inference="COORDINATES: protein motif:HMM:NF014040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HFA9080209.1"
                     /translation="MRFERTVHVSAEKPGISGILDLVEMDTKTGRLKPVEYKRGKPKP
                     DPVDEIQLCAQGLCLEEMTGQTVSEGALWYMQTRHRVPVVFSDGLRPLQ"
     gene            5404..5589
                     /locus_tag="WM481_000857"
                     /pseudo
     CDS             5404..5589
                     /locus_tag="WM481_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
     gene            complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="WM481_000858"
     CDS             complement(5629..7557)
                     /gene="rpoD"
                     /locus_tag="WM481_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFA9080210.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKICKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEVSAANLAELKQKVIGHFAQIEKDYKKMIGCLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLQWIEEEIAKGRVWSNALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            complement(7744..9516)
                     /locus_tag="WM481_000859"
     CDS             complement(7744..9516)
                     /locus_tag="WM481_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFA9080211.1"
                     /translation="MIPSDFIDELLAKTDIVGIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLVQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETIDRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            complement(9661..12411)
                     /gene="secA"
                     /locus_tag="WM481_000860"
     CDS             complement(9661..12411)
                     /gene="secA"
                     /locus_tag="WM481_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFA9080212.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRVLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSETGHEH
                     AEQILTQMGLLAENDSLYSAANISLMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSRLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDIGDLMQEIR
                     SDAVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLQEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQAIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAAVEEQPVGNIQSIHSES
                     PDIEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            12543..12965
                     /locus_tag="WM481_000861"
     CDS             12543..12965
                     /locus_tag="WM481_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HFA9080213.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFDSAAAKLEERHPELAEALEELVRKYGA"
     gene            13248..13799
                     /gene="azu"
                     /locus_tag="WM481_000862"
     CDS             13248..13799
                     /gene="azu"
                     /locus_tag="WM481_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFA9080214.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            13894..14403
                     /locus_tag="WM481_000863"
     CDS             13894..14403
                     /locus_tag="WM481_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9080215.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQIAMLSGYIAENGC
                     NQLVLQTIRQISRYFNVAAPLHHEDEEENFFPLLLQYAPQAREGVDELLRQHIGLYDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVEGYDVHLAIEEPLFDMGKTFIPEEKLTEIG
                     KIMAARRCK"
     gene            14437..15057
                     /locus_tag="WM481_000864"
     CDS             14437..15057
                     /locus_tag="WM481_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase"
                     /protein_id="HFA9080216.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAADLSEPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTEKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     RLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            15170..16315
                     /gene="dapE"
                     /locus_tag="WM481_000865"
     CDS             15170..16315
                     /gene="dapE"
                     /locus_tag="WM481_000865"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFA9080217.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAVNPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTETGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARTAIAETCGVEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYERILARLLAEKAV"
     gene            16735..17550
                     /locus_tag="WM481_000866"
     CDS             16735..17550
                     /locus_tag="WM481_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFA9080218.1"
                     /translation="MGALGGIVQHHTALYRSVAVYEPTVVIVRRGCKKLRWAGRELRI
                     AAGEAVGLAGGQTLDVINIPDSDGLYQAQWIAFEQETVERFAAQYGTAQAVCDAVKLP
                     HPGRMGAAFDYAAAVLADEEVPHNAAEAALCGVLAWLQHDGIGFAVYGGVNLMRQIRK
                     LITADMAADWSSAMLAQRLNCSEAALRRRLARQDTNFRTLLTDVRMMRALTLLQVTQW
                     SVAQIAGAVGYDCPSRFSARFKELFGCVPSVVRSEAEPAAYRRTGQVSVGVRP"
     gene            complement(17528..18085)
                     /locus_tag="WM481_000867"
                     /pseudo
     CDS             complement(17528..18085)
                     /locus_tag="WM481_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273197.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            18435..19187
                     /locus_tag="WM481_000868"
     CDS             18435..19187
                     /locus_tag="WM481_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFA9080219.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPYPNLTECEAGQF
                     ERDFWDNIGCAPEEYVRIRRAIRLLELRYPDSLNELVCAAIATPLGEMLAVFGVKGLC
                     LLEFVGQKYLEQEIAAVQKALRGRFVFREDGRMQFLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDVLLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVMIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            19378..19453
                     /locus_tag="WM481_000869"
     tRNA            19378..19453
                     /locus_tag="WM481_000869"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19411..19413,aa:Asn,seq:gtt)
     gene            19584..20594
                     /gene="waaF"
                     /locus_tag="WM481_000870"
     CDS             19584..20594
                     /gene="waaF"
                     /locus_tag="WM481_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFA9080220.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRELGRRGYDRVIVLPGSLKSAVIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISIETFGLNLGKPVLAFCPGAEFGPAKRWPARHFAELGKHYSEAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            20649..21095
                     /gene="smpB"
                     /locus_tag="WM481_000871"
     CDS             20649..21095
                     /gene="smpB"
                     /locus_tag="WM481_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFA9080221.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLKQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            21460..23499
                     /locus_tag="WM481_000872"
                     /pseudo
     CDS             21460..23499
                     /locus_tag="WM481_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(23482..23778)
                     /locus_tag="WM481_000873"
     CDS             complement(23482..23778)
                     /locus_tag="WM481_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080222.1"
                     /translation="MVGLARARGRGELDRIEQMDLDFFHRTRARYLELVKDNPKAVVI
                     NAEQTIELVQAEIESAVKIGGNYTKNDRTFKIIKSAVNFPADFILLQFNLKRPL"
     gene            complement(23828..25195)
                     /locus_tag="WM481_000874"
     CDS             complement(23828..25195)
                     /locus_tag="WM481_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9080223.1"
                     /translation="MPDLHTEFSRLLPADEIAEPSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFPPEILSNEVYRYEN
                     DINSTVYRNVLARNGTIAAEHGIGIIKKQWLPAVRTPSEIALMKSIKQHLDPYNIMNP
                     GKLLP"
BASE COUNT         6144 a         5928 c         6848 g         6452 t
ORIGIN      
        1 gtgaaccgct gccgccgtcg gcgttatcgg aaagcgaggc aaaccgcgat ttgggctgga
       61 tgctgcacga tattgatttt gatcacggca acacgccgca ttttttccgc acgcaaatga
      121 aagacggctt gattgatgtg ccgccgtttt acgccgagga ggtgaaagca tgattcttgc
      181 gtcccttgtc cgctattacc gccgtttggc aacggaaacc gatgaaacgg gcaacccgaa
      241 agtgccgtct tatggtttta gcgaggagaa aatcggctgg attttggtgt tggataaaga
      301 aggtcgtctg aaaaccgttg tgccgaatct gactgccgat aaaaagccgc agccgaagct
      361 gatgagtgtg ccgcgccctg aaaaacgcac gtcgggtatc aaaccgaatt ttttgtggga
      421 taaaaccgcc tacgcgcttg gcgtggaagc caataaaaac aaagccgaag ccaaagaaaa
      481 accgtttacg ccgtctgaaa aaacctttga agccttcaag caataccatc tcgatttact
      541 gcaaaacagc gaagacgaag gtttacaagc cttatgccgt tttctgcaaa actggcagcc
      601 tgcacatttc gctgccgaaa acctgcctgc cgaaatgctt gattccaaca ccgcattttc
      661 tcttgaaaaa ccgaccgctc ttatccataa acgcgaagcc gcgcaaacct tgtgggcagg
      721 ctgcctgaaa agtgatgaag cactcgaaag cttatacctg attagcggcg acaccgcgcc
      781 gattgcacgg ctgcatccgg cgattaaagg cgtgtttggc gggcaaagct ccggcggttc
      841 gattatttcg tttaataaag aagccttttc ctctttcgga aaagagcaag gcgcaaatgc
      901 gcctgtttcc gaacaatccg cctttgccta caccaccgcg ctgaactatc tcttgcgccg
      961 cgaaaataat cactgcctga ccataggcga tgccagcacg gtcttttggg cggaagcgga
     1021 tgatatagtg gattaaattt aaatcaggac aaggcgacga aaccgcagac agtacaaata
     1081 gtacggcaag gcgaggcaac gccgtaccgg tttaaattta atccactata acgccacggc
     1141 gcaggctgcc gaaggcttct tcgcgcaagt gtttacgccg tcggatgacg aacaagaaag
     1201 cgccaaaatt ttcaacgtat tggaacaaat cggcaaaggt cgtccgctgc aagaaattgc
     1261 gcctgaactc tctcccaata cccgttttta catcttgggg cttgccccca atgctgcgcg
     1321 gatttctgtt cggttttggc tggacaccac atttgggcag ctggcggaaa acttggcgca
     1381 tcattggcaa gatttagccc ttgagccttg tgcgtggaaa acgccgccgt ctatttggcg
     1441 gctcttgttg caaactgccg tgctgggcaa aagcgaaaac atccaaaggc ttggtaaccg
     1501 atgtctgcct gaaacgcaaa atccgtaatt ttgtcgaaat cagcagcgaa aacgaagccg
     1561 gttacgaaat ctacgtcaaa gaaaaaagcg tgctgaacct gcaaaacaaa cgcgcttatg
     1621 aggcgcttgg cattgagtcg gaagccaaaa actgccgaag gacgaagcca aagcccgtga
     1681 tattaccgct tggatgtgta aaaacttctt tgacatccgc accttcggcg ccgtgatgac
     1741 caccgaagtc aacagcggac aagtgcgcgg cccggtacaa ctggcgtttg cccaatccat
     1801 cgacccgatt gtgccgccgg aagtttccat cacccgcatg gcggtaacca atgaaaaaga
     1861 cttggaaaaa gaacgcacca tggggcgcaa atacatcgtc ccttacgcgc tctaccgcgt
     1921 gcatggcttt atctccgcca accttgccgc caaaaccggt ttttcagacg acgacttagc
     1981 caaactctgg caagccctga cgttgatgtt tgaacacgac cgctccgccg cccgtggcga
     2041 aatggcggca cgcaaactgg ttgttttcaa acacgacagc gcactcggca gccagcctgc
     2101 acataaactg tttgacgccg tgaaagtcga acgcgtaaac ggcgaatcag gtacgcccgc
     2161 aagcggtttt ggcgattaca aaatcagcgt ggtttcagac gggctaaatg gcgtgagtgt
     2221 ggaagagtat ttgtaagttt tcaataaatc aggagtaacg atatgaaata ttggctagtt
     2281 ggagcctctt ggggagggca ggggcatcaa gatcaatttt ttgttgaaaa tggttactgg
     2341 gttttggagt gggaatcctc tcaacaacca gatcaatttg caaaaggaga aaaaatccaa
     2401 gtaggtgatc gaattgcaat caaacgcatg aaaggtcaag gatcgtctga aattaagatt
     2461 ttacatattg gaatcgttaa gggggttatt tctgaaacga acaaaattat ctgtgtcgtt
     2521 gattggatag ttaaaaattt agatagaaat gttgaaagta gaggatgctt taaaagtatt
     2581 catgatccat atgataaaga tgaatggata gaaaaaattt tttgtttgta gcaaaagatg
     2641 accgcacttt taaccgaaac ccaaagggaa aatcaggata cgcgcctgat tcccctttcc
     2701 gccctgcaac actacgcctt ctgcccgcgt caatgtgctt tgattcacaa cgaacaggcg
     2761 tgggcggaga actatttgac cgcgcaaggc aaagcgttac atgagcgggt ggattcggac
     2821 gagccggaaa cgcgcaaggg cgtacgtttt gagcggacgg tacacgtttc ggtagaaaaa
     2881 ccgggcatca gcggcatatt ggatttggtg gaagcggata cgaaaacagg ccgtctgaaa
     2941 cctgtggaat acaaacgagg caagcccaaa cctgacccgg gggatgaaat ccagctttgc
     3001 gcccaaggtt tgtgtttgga agaaatgacg gggcaaaccg tttcagaggg cgcgctgtgg
     3061 tatatgcaaa cccgccaccg cgtccccgtt gtgttttcag acggtctgcg cgcccaaacc
     3121 ctcgccacca tcgccgctgt gtgcaaactc ctaaacagcg gccaaacccc gccgcccgac
     3181 tacggcaaac gctgcaaagc ctgctcgctg gcggagattt gccagccgga gttgctggga
     3241 aaacgggata ggagtgtggg atatgtgaag gggttgtttg gggagtaata ttttatttga
     3301 aaaaaattat tacgttaatt aacttgaatc ttccaaatta atcgcaattt ctgaaaacga
     3361 gcaatcatgc tcaattgtaa ttacacattt taaggagtcg taaatgacaa gaaatataga
     3421 acatcaattt agcaatctat ctgatgttgg tgaaaaacta gagttagaaa atcctactgt
     3481 agagaatgtc gtagatattt tagtggatat tggacatgat gaccgagttt atacttttca
     3541 tgatgacttt ctcgggttaa aaagcggttt gccacaagac ttattgtcga aacatatcga
     3601 cgagcttgaa gaaggtgatt ttgcagatag atattcagat gaaattgata aaatactgga
     3661 taatgcaaat ataatattct atcaccttga acgagagctt agtgaagatg atttagagga
     3721 aatacgagaa gaaagagaaa gactgggttt agaagacgat tagtatactt gttgtcatag
     3781 aatagattca atacatacag gttgggttat gctgttttac ccaaccagct aacctcaggg
     3841 catatttaat attatttaat tctgaataat attctattga agtttcaaga tatctaagga
     3901 aaaacatgac acaaaaaaat gattttaaaa atgctaattt ttatgggaat actcaaatag
     3961 ttaatggtga tgttcataat gtacatactc aaaatacacc aaattcaata aatgaaaacg
     4021 aaatagccac atacaccccg gaaccaattt ggagaagccc cattacgatg gcttggctga
     4081 catgggctag ttttctgatt tctttagcag ggctattacc tatttataat tgtatagtta
     4141 aacccatatt atatttagta aatggtaaaa ctatgataca aagtgaaata aataatactt
     4201 atatatttat tttaatagct attgctgtat tatttcttat cactattaca ttacgcagaa
     4261 ttactaagta tcaaactaga tacccattag tttttaatta tgctattagc ggtatggggc
     4321 atcgtttaag tctagagaaa attagtattt ctcaatgtcc acagtgcggt ggagaaatga
     4381 aatattataa taagccaatc gagtgggaga atatacgtta ttctgatggt agcaagaaaa
     4441 gaagggtaac aagaactaca ccagcattag agtgtaaaag aaatcgtgag cattggtata
     4501 aagttgatcc ggcagaagat aaaatctaat ttttagctag cccctcgttt aaccccaaaa
     4561 gcacggtcaa aacctaagga gttttcccat gcgcaaactg caaaacacac tctacatcac
     4621 cacccaaggc agctatctgc ataaggagcg ggagacactg gtggtggagc aggagcgtaa
     4681 gaaggtggcg cagttaccgg tgcattccat cgggcatatt ttctgttttg ggaatgtgct
     4741 tgtgtcgccg tttctgttgg ggttttgcgg cgaaaataat gtgaatttgg cgttttttac
     4801 cgaaaacggg tgttatttgg gacggcttca ggggcggcag aacggcaatg tgctgttgcg
     4861 tcgggcgcag tatcgggtgt cggagcaaag tcccatgccg attgcgtgca atatcattgc
     4921 ggcaaagatt cgggcgatcc ggaaacgtgc aagggcgtgc gttttgagcg gacggtacac
     4981 gtttcggcgg aaaaaccggg catcagcggc atattggatt tggtggaaat ggatacaaaa
     5041 acaggccgtc tgaaacctgt ggaatacaaa cgaggcaagc ccaaacctga cccggtggat
     5101 gaaatccagc tttgcgccca aggcttgtgc ttggaagaaa tgacggggca aaccgtctct
     5161 gagggcgcgc tgtggtatat gcaaacccgc caccgtgtcc ccgtcgtgtt ttcagacggc
     5221 ctgagacctt tgcaataaca taggttacta aaattttatg ctcaatctca ttttcaaaat
     5281 gcaaaacttt tctgattttt cctacttttt gctcaatatt aggaaggttt taggcaattg
     5341 aaaatttttt ggcgcatttt tatgcgtcaa atttcgttaa cagactattt ttgcaaaggt
     5401 ctcggcctac gcgcccaaac cctcgcaact atagccgccg tccgcgaact cttaaacagc
     5461 ggacaaaccc cgccgcccga ctacggcaaa cgctgcaaag cctgctctct ggtggaagtc
     5521 tgtcagccgg agttgttgga gaaacgggat gggagtgtgg gatatgtgga agcgttgttt
     5581 attgtgtaag aaaaaaccgc aggtatttga aacctgcggt tttttggttt acagcttgct
     5641 gtcttcgctg tccaagaaac ttctcaaacg gtcgcttctt gtcggatgcc gcagcttgcg
     5701 gagtgctttt gcctcgattt gacggatgcg ttcgcgcgtt acgtcaaact gtctgccgac
     5761 ttcttccagc gtgtggtcgg tgttcatatc gatgccgaaa cgcatacgca ggacttttgc
     5821 ctcacgcggt gtcaggcttt cgaggatttc tttggttact tcgtgcaggc tggtgtacat
     5881 tgccgcatcg gccggcgcaa cattgttggc atcttcgatg aagtcgccca agtgcgaatc
     5941 gtcgtcgtcg ccgatggggg tttccatcga aatcggctct ttggcgattt tcatgatttt
     6001 gcggattttg tcttcgggca tctgcatcag ttcggcaagt ttggcggaat cgggttcttc
     6061 gccggtttct tgaaggtgtt ggcgcgagat gcggttcatc ttgttgatgg tttcaatcat
     6121 atgtaccgga atacggatgg tacgcgcctg atcggcaatc gagcgggtaa ttgcctggcg
     6181 gatccaccag gttgcgtagg tggagaattt gtagccccgg cggtattcga acttgtcgac
     6241 cgccttcatc aggccgatgt tgccttcctg aatcaaatca aggaactgca agccacggtt
     6301 ggtgtatttt ttggcaatgg aaatgacgag gcgcaagttt gcctgaatca tttcctgttt
     6361 ggcggctgcg gtttcttttt cgctcgacac catatttttg ttgatttctt tcaactcttc
     6421 gatggaaatg cgggtttctt tttccatatc cgccaactcg gtttgttttt cgaggatggc
     6481 gtggcggaag cggtcgagtg cgttgctcca aaccctgcct ttggcgattt cttcttcaat
     6541 ccattgcaga ttggtgattt cgggcaggaa gttttggatg aagtagtcgc gttccatatg
     6601 gacgcggtcg aggcagatgt cgcggatttc gcgttcgagt ttgcggatgt tttctacttt
     6661 tccgcgcagg ctgctgctga ggctgtcgat ttgccgggtg gcgaaacgga cttccagcag
     6721 tttgttggca atcgcgtcgc ggtaggcgag atagtctttg tgccggctgt ggtgtttttc
     6781 caaacagccg atcatttttt tgtagtcttt ttcgatttgg gcaaagtggc cgatgacttt
     6841 ttgtttcaat tcggcgagat tggctgccga gacttcatcc gcatcttctt ccgattcttc
     6901 gtcgtcttcg ttttcgtccg aattgtcgtt ggaaggtttc tcgggtgctg tggtttccaa
     6961 gtgccccaag cccaattcgt tgagcaatac ttcattcggg tcgataatgg cttctacgac
     7021 ttcgtcgacg cggatttcgt ctttgcagat tttttcgatg agttctaaga tttccgcaat
     7081 ggaacccggg caggcggaga tggcctgaac catatttttc agggcgtttt cgattttttt
     7141 ggcgatgatg atttcgtctt cgcgggtcag caggtcgacc tgtcccattt cgcgcatata
     7201 catacggacg gggtcggtcg tcctgccgaa ctcggaatct gcgctggaaa gggcggcctc
     7261 ggcttcttcg acggcatcat cgtcggtaac ggcggcattg tcgcttaaca atatgtcttc
     7321 cgcatcaggg gcgtgttcgg taacttggat acccaaaccg gaaatcatac tgacgatgtt
     7381 gtcgatttga tcggcatcgg acatatcgtc gggcagtgcg tcgttgattt cggagtaggt
     7441 gatgtagccg cgttctttgc ccataatgat gagttggcgc aggcgcgcac gttgttcctc
     7501 aatcgtcagg gggcgggcat cgtcttggta ttcttcgtga ttttggtttt tggacattgt
     7561 ctgctttctg ttggcaagat gccgacggtt tgcgtttgga cgcacctgaa ggcgtggcaa
     7621 agtagggtac tctgatgccg tctgaaggct ttttgggatg ggtgttctgc aggtgtcgtt
     7681 gttgcagccg gggtgaatgc ttttcagacg gcataacggt ttacagatgg cggaatccgc
     7741 cggtcaattt tgttttgcgg tcagcagcga cagcaaaagt tttttctcgc tttcatttaa
     7801 gccggattgc aggctttttt gttttaatgt ttcaatttgg ctgtatttta actcattgag
     7861 cagttttttg atgccgattt ggaagttttc gcaatcttct tcgccgccgc cttccatttc
     7921 ttccgattga agggccgacc ggaagatgcg gtcgattgtt tcttcgtagg gcgaaccgcg
     7981 catatgttcc aaaacctgtg cggttgccgg cacggaagga tggtttttaa tggtttcggc
     8041 gaggttggca aggcaggcga aatcgccgtc caacgccaga taatcgggca ggtctatata
     8101 tgcagcccaa tccggattta tcaagaggct gcggatttgc cgttgcacca atgtcggcat
     8161 aacgggctgt ttgacggaaa tcggaggcag tttgtagttt ttttgtttga cgtgccgctt
     8221 tggcgcttcc tgtccgagca gttgcgcgag gttgtcgggg tcgatgccga ccagctcgct
     8281 aagccgttgt tttaacaaat aagccaatgc cggtgcggta atctgcacca aaagcggcga
     8341 actggttttc accaattccg ccttgccttc ctgcgtattg agatgaatgc cgtctgaaag
     8401 gtgttcccag aaatattccg acaaaggctt gctttgattc agaagcgcgt cttcaaattg
     8461 cgctttgccg taggcgcgga tgtagctgtc ggggtcgtgt tcttccggca ggaataaaaa
     8521 atgcagcgat ttgtcgtctt tcaactgcgg cagcgcgttt tccagcgcgc gccaagccgc
     8581 ttttcgcccc gcgctgtcgc cgtcgaaaca gaaataaata ctgtccgcct gccgcatcag
     8641 gattttgacg tgttccgccg tcgtcgccgt gcccaaggcc gccacaccgt agcccacgcc
     8701 gaactgtgcc agcgcgacca cgtccatata gccttcgacc accaaaatcc gttctgcctc
     8761 ttttacagca gcacgcccct catacaagcc gtaaaggttt ttccccttat cgaacaaagg
     8821 cgtatcgggc gaattcaaat atttcggttt cgagtcgtcc agcaccctgc cgccgaaacc
     8881 gatgacctgc ccgcgcggat tgcggatggg gaacataatc cgatggcgga agcggtcgta
     8941 atgccgcccc tcattgtcaa tcaccatccc cgtatccacc aacgcggtat tcggatacgg
     9001 ttggaacact tgcgccaaag gctgccagcc gtcgggcgcg tagcccaaac cataatgcgc
     9061 gatgacttcc gcgctcaagc cgcgtttgtc caaataagct ttcgcagccg gattgaattt
     9121 cagctgctgc gcgtaaaaat cagctgccgc cgccgtcgtt tcttccagcg tctgctgttt
     9181 tttcttgcgc tcggcacgga cttcgggatt atcgttctgc ccgcgcactt taggcacaat
     9241 catacctacg cggtcggcaa ggaactgaac cgcctccgga aacgacagtc cctgatgttc
     9301 catcacaaaa ccaatcgccg aaccatgcgc cccgcaactg aaacaatgat aaaactgctt
     9361 ggtcggactg accgaaaacg acggtgtttt ttccttgtgg aacgggcaac acgccatata
     9421 gttcgccccg ccttttttca gcggaacctg ctcgtcgatg atgccgacaa tatcggtttt
     9481 ggccagaagc tcgtcaatga agtcggatgg aatcatgatg tggggggacg ggatgtctgt
     9541 tttcggtacg gacggtacga aatcgggatt tttcggaatg gcggtcggat gtggcggttc
     9601 aggcaaaatg ccgtctgaag ccggaaaccg gatgttcaga cggcatttgc gttcaaacgc
     9661 ttaagccaat ttgccgtggc attgtttgta tttcaaaccg ctgccgcagg ggcaggggtc
     9721 gttgcggtgg acgatttgtc cccgcgcttc caaggcttcg gggctgaaat ccgtcccatc
     9781 gggattaaag gcttcggtaa ccagatcggt ttgcgactga cccaaaagtt cttctatatc
     9841 gggcgattcg gaatggatgg actggatgtt gccgacgggt tgctcttcaa ccgccgcgac
     9901 agggttttgt tcgatttgaa ccgaagtaag cagggaggcg atatggaatt tgatgccgtt
     9961 ccacaggtct tggaacatgg taaaggcttc gcgtttatat tcctgcttcg gatttttctg
    10021 ggcatagctg cgcaggtgta tgccttgtcg caggtagtcc atggcggcaa gatgttcgcg
    10081 ccattggttg tcgatggcct gcaacatcac gttgcgctcg aaatcagcca ttgcctgctt
    10141 gccgaccagt tcggttttgg cggcatattc gttttcgata cgttcgatca ggcgctcttt
    10201 gatgtcttga ccgtcaatcg cattgtccgc cttcagccag gattggatgt cttcctgaag
    10261 cctgaattcg gcagccaggc ggttttccag tgtcgggatg tcccattgtt cttccatgct
    10321 gtcgggcggc atataggtat ccacgaggtc gctgacggca tcagaacgga tttcctgcat
    10381 caggtcgccg atgtctttgc tggtcagaat ttcgttgcgc tggctgtaaa tgactttgcg
    10441 ctgttcgttg gcaacgtcgt cgtattccaa aacctgtttg cgcatatcga agtttctgcc
    10501 ttcgactttg cgttgcgccc cttcgatttg gcgcgtcagc aggttgtgct cgatggcaac
    10561 gccgcgttcg ggggcgaggc ggttgaggat ggcggcggcg cggtcgagtg cgaagaggcg
    10621 caacagcggg tcttcaaacg agaggtagaa acggctggat ccggggtcgc cctgacggcc
    10681 ggaacgtccg cgcaactggt tgtcgatacg gcggctttcg tggcgttccg taccgatgat
    10741 gtgcaaaccg cctgcttcca tcactttatc gtgttctgcc tgccagccgt tttcgagtgc
    10801 ggcgatttgt gcctgtttct cttcgtcgct caaggtttcg tcggcgcgga tggcatcggt
    10861 ttggtgcttc aggttgccgc ctaaaacgat gtccgtaccg cgtcccgcca tattggtggc
    10921 gacggtaatt gcgccgactt tgccggcttg ggcgacaatc agggcttcgc gttcgtgttc
    10981 tttggcgttg aggacgttgt gcggcagtcc ggctttttgc agaaggcggg agaccagttc
    11041 ggagttttcg atgctggtcg taccgacgag gacgggctgc ccgcgtttgt ggcattcctc
    11101 gatgtctttg acgacggctt cgaatttttc ttcggcggaa cggaaaatct ggtcgttgaa
    11161 gtctttgcgc tgtacggggc ggttggtcgg gatgatgacg gtttcgaggt tgtagatgct
    11221 ttggaactcg aaagcctcgg tatctgccgt accggtcata ccggagagct tggtgtacag
    11281 gcggaaatag ttttggaagg tgatggaggc gagggtttgg ttttcgcgtt tgatttccac
    11341 gccttctttg gcttctacgg cttgatgcag gccttcggac cagcggcggc cggacatcag
    11401 acggcctgta aattcgtcaa cgatgacgat ttcgccgtct tggataacgt aatgttggtc
    11461 tttgtggaac agcgagtgcg cgcgtaatgc tgccataagg tggtgcatca gggagatatt
    11521 tgccgccgag tacagcgagt cgttttctgc cagcagcccc atttgggtca ggatttgttc
    11581 ggcgtgttcg tgacctgttt cgctcaggat gacctgatgt gccttttcgt cgacccaata
    11641 gtcgccttcg ccttcttctg tctcttgacg gacgaggtgg ggcggaacgg tgttcatgat
    11701 ttggtagagc tggatgttgt cgtctgcctg accggagata atcagcgggg tgcgcgcttc
    11761 gtcaatcaag atggagtcca cttcatcgac aacggcaaaa ttcaattcgc gctgcacttt
    11821 gtcgtattgg tcggttacca tattgtcgcg caggtagtcg aagccgaatt cgttgttcgt
    11881 gccgtaggta atgtcggcag cgtaggcgtt ttgacggtcg aacggctgca tatctgaaat
    11941 aatcacgccg acggtcagac cgaggaaatt gtagagcggc tccataatgc ctgcgtcgcg
    12001 cgaggcgagg tagtcgttga cggtaacgac gtgcacgcct ttgccggcca gcgcgttgag
    12061 atagacggcg agggtggcga ccagggtttt gccttcgccg gtacgcattt cggcgatttt
    12121 gccgtcgtgc agcaccatac cgccgataag ctgcacgtcg aagtggcgca taccgagtac
    12181 gcggcgggac gcttcgcggc agacggcgaa ggcttcgggc aaaatgccgt ccaaagtctg
    12241 accgtcggcg aggcgttgtt tgaattcggc agttttggct tgcagatcag catcgcttag
    12301 ggcttgcatc tgttcttcga gcgcgttgat tctggcaacg gatttacggt attgtttcag
    12361 caagcggtcg ttgcggctgc cgaagatttt cttggcaatg tttgtcagca tgaataattc
    12421 ctgcgcctga tataatagtg gggcgaaaac gcgattttaa cacaagcgtt tgtgcaaagt
    12481 gctaatgccg tctgaaataa aggcgcgttt gcggtatttc aatcttttgg ggtgtatgtt
    12541 ttatgaattt ggaacagttg ggcaggcggg acgcgctgct ttccggactt ttgaaacagg
    12601 cgggacagtg gcggcggttg gatgctgccg tgaaaaaact gctgcctgcc aacctgcacc
    12661 cccactttca gacggcatgt atcgaagacg gcaggcttgt ccttttggcg gcaaacaata
    12721 tggcggcatc gcgtttgaag atgattgccc cgtcggtatt gccgcagctg gcagggcttg
    12781 atgcttcgat acggtctgtt tcggtcaggc tggttccaaa accggaaaaa ccgccgaaaa
    12841 ccaataccct gcatttgagt aaggctgcgt tggagagttt cgattcggcg gcggcaaagt
    12901 tggaagaacg gcatcccgaa ttggcggagg cactggagga gctggtcagg aagtacgggg
    12961 cataaggcgg ccggcaggga tgttgtaaat atccgttaat atttgcgcgg cgggggacgg
    13021 ctgccctaat actaaagaaa taggttcggg gtaagattgc cctttttttg ggtaaacgat
    13081 tgtaaacttg caaacaggct ttgatttcaa atgaagtttg tagcaaaatg ccgccccgaa
    13141 acatctgttt gtgcaacacg gcggaatctt tttcaaggtt ttgttaatgg cggttgcact
    13201 ttgatttccg taaaaccgaa tattatttta tcgattggag atttaccatg aaagcttatc
    13261 tggctctgat ttctgccgcc gttatcggtt tggctgcctg ctctcaagaa cctgccgcgc
    13321 ctgctgccga ggcaactcct gccggtgaag cacccgcttc cgaagcgcct gccgccgaag
    13381 ctgctcctgc agatgctgcc gaagcccctg ctgccggcaa ctgtgcggca actgtcgaat
    13441 ccaacgacaa tatgcagttc aacaccaaag acatccaagt cagcaaagca tgtaaagagt
    13501 tcaccatcac tctgaaacat accggtacgc aacccaaagc cagcatgggt cacaaccttg
    13561 tgattgccaa agctgaagac atggacggcg tatttaaaga cggcgtaggt gctgccgata
    13621 ccgactatgt caaacctgac gatgcgcgcg ttgttgccca caccaaactg atcggcggcg
    13681 gcgaagagtc ttccctgact ttggatcctg ccaaattggc tgacggcgac tacaaatttg
    13741 cctgcacctt cccgggtcac ggtgctttga tgaacggcaa agtgactttg gtcgattaat
    13801 ccgcttaaag tttcaaaaga cagacagcct gctttgtgca ggctgtttta ttataaaatg
    13861 actgcttgaa aagtccccgt tgagaacgaa aacatgaatc cgtttgaaac ccaaagcgtt
    13921 acctttgccg aaccgattga aatgctgtat gcctgccacg gcaaagtgcg ccgtttttgc
    13981 ggccagattg ctatgttgtc gggctatatc gccgaaaacg gctgcaatca gcttgttttg
    14041 caaaccatcc gccaaatctc ccggtatttc aacgttgccg cgccgctgca ccatgaggac
    14101 gaagaagaaa actttttccc gctgctgctg caatacgcgc cgcaagcccg tgaaggcgtg
    14161 gacgagctgt tgcgccaaca tatcgggctg tacgacaact gggcggctgt ttccgccgaa
    14221 tttgccaaac tcgaagcaga caacgcttat atccccgatg cggaagcgtt caaacgcttt
    14281 gtggagggat atgatgtgca tctggcgatt gaagagcctt tgttcgacat gggcaaaacg
    14341 tttatccccg aagagaagct gaccgaaatc ggcaaaatca tggcggcgcg ccggtgcaaa
    14401 taatgccgtc tgaaacaatc gtctcaagga tatattatgc tgacgcccaa aagctgcgat
    14461 ttgttcaata tcccgttttt ccagtttgcc cagctcaaaa aataccagcc cgagagcatt
    14521 ccgcaaatca aagccgacta caaggaaaac tggcagatat ggcagcagtt gatacagcag
    14581 gtcgccgcag atttgagcga acctttcgcc ccgccgcata tcgaacgctg gtgtaacggc
    14641 tggcaggttc gcgcccattt cttcgcctac ttcaaatacg cccaatacaa aaattccgcc
    14701 gccatcctgt cgatactgct caaccgccgc cgcctgagcg tcagcttgga ctggcattgc
    14761 tacaaagccg acgtgtcgcc gattgcgctg cccgagtaca accgctggct ggatgatttc
    14821 gataccgaaa aatacgccgc attcgatatg tggcacggcg cggaaagcga atatgacgac
    14881 taccgcaccg tcgcacaaca aagcgaatcc gaccgaaggt tgcaaaacga cgaagacttt
    14941 ttctgcatcg gcaaacacat tgagcgcgac gatttgggca agcaggatgt agcaaaatgg
    15001 atagccgaaa cggtggaaga tttactgccg ctttacgaag cctgtcacgg caaatgattc
    15061 agaaaactga cgggtacgaa tgcccgtctt atacaccaat ccaacccgga ggtcgtctga
    15121 aaatcagctt caggacattt cagacgacct gatttccaaa gtgaacaaaa tgaccgaaac
    15181 ccaatcccta gaactcgcca aagcgttgat ttcccgcccg tccgttaccc ccgacgaccg
    15241 agattgccaa aaactgcttg ccgaacgcct gcacaaaatc ggttttgcgg ctgaagaact
    15301 ccatttcggc gacaccaaaa acatctggtt gcgacgcggc acgaaagctc ctgtcgtctg
    15361 ttttgcaggg cataccgacg ttgtgccgac aggtcctgtt gaaaaatggg attcgccccc
    15421 gttcgaaccg accgagcgcg acggaagatt atacgggcgc ggcgcggcgg acatgaaaac
    15481 cagcatcgcc tgtttcgtta ccgcctgcga acgctttgtt gccgaacatc ccgaccatca
    15541 aggcagcatt gcactcttga ttacttccga cgaagagggc gatgcactgg acggcacgac
    15601 caaagtcgtc gatgtgttga aagcacgcgg agagttgatt gactactgca tcgtcggcga
    15661 accgaccgcc gtggacaaat tgggcgatat gattaaaaac ggccggcgcg gttcgctgtc
    15721 gggcaatctg accgtcaaag gcaagcaagg gcatatcgcc tatccgcatt tggcggtgaa
    15781 tcccgtgcat acttttgccc cggccttgtt agagttgacg caggaagttt gggacgaagg
    15841 caacgaatac ttcccgccga ccagctttca aatttccaat atcaacggcg gcacaggcgc
    15901 gaccaacgtc attccgggcg agctgaacgt caaattcaat ttccgcttct ccacagagtc
    15961 caccgaaaca gggctgaaac aacgcgtcca cgccattttg gacaaacacg gcgtgcaata
    16021 cgatttgcag tggtcgtgtt cgggacagcc cttcctgacc cacgcgggca aactgaccga
    16081 cgtggcacgc accgccattg ccgaaacctg cggcgttgag gccgaattgt ccaccaccgg
    16141 cggcacttcg gacggacgct tcattaaagc cattgcgaaa gaactcatcg aattaggccc
    16201 gtccaatgcc accatccacc aaatcaacga aaatgtgcgg ctggacgata ttccgaagct
    16261 gtcggcggtg tatgagagga tattggcgcg gttgttggct gaaaaggccg tctgaaaaga
    16321 agatgggctg gttgtcggat atggatgtcc gacaatctga ttagacccaa catgagtttg
    16381 agataacgca ttgattttta tgtagagcga tttatttgaa cggtaatcca ttccctctcc
    16441 cgtgggagag ggttagggag agggcattgg gacggcaacg gaaatattgt ctgtatctgc
    16501 cacgttttac cctccctccg accctcccca caggggaggg agctaagttg caggcacacc
    16561 gacggaatat ggttgaaacc ctgcccttca gggcagtaga attttggttt attttttggg
    16621 aagtaacccc ttccaaatca gggtaaacag ggctgcgcct tatgtgcggc cctgtgtgtt
    16681 gaaacattga ggaatataac cgtgccgtca aattactttc atacgcgtga acgattgggt
    16741 gctttaggcg gcatcgtgca acatcatacc gccttgtacc gcagcgtggc ggtatatgaa
    16801 ccgaccgtgg ttatcgtgcg gcgcggatgc aagaagctgc ggtgggcggg gcgcgaattg
    16861 aggattgcgg cgggtgaggc tgtcggcttg gcaggcgggc agacgcttga cgtcatcaat
    16921 attcccgatt cagacggcct ctatcaggcg caatggattg ctttcgaaca ggaaaccgta
    16981 gaaaggttcg ccgcacaata cgggacggcg caggcagtgt gcgatgcggt gaagctgccg
    17041 caccccgggc gtatgggcgc ggcgtttgat tatgcggctg cggtactggc ggatgaagaa
    17101 gtgccgcaca atgcggcgga ggccgcgctt tgcggcgtgt tggcgtggtt gcagcatgac
    17161 ggcatcggtt ttgccgtgta cggaggcgtc aacctgatgc ggcaaatccg caaactgatt
    17221 actgccgata tggctgcgga ctggtcgtcg gcaatgctgg cgcagcggct taattgcagc
    17281 gaggccgcgt tgcggcggcg gttggcgcgg caggacacga atttccgtac gctgctgacg
    17341 gatgtacgca tgatgcgcgc gctgacgctg ttgcaggtta cgcaatggtc ggtagcgcag
    17401 attgccggcg cggttggcta tgactgcccg tcccgtttca gcgcgcgctt caaagagctg
    17461 ttcggttgcg taccgtcggt ggtccgctcg gaagcggagc cggcggcata tcggcgcacg
    17521 gggcaggtca gcgtcggtgt tcgaccgtga atttcgcttc gcccaagctg tttgccttag
    17581 tggagaaacc gaccagcacg ggtgtggatt cggcggtgat gttcggcagc ttggcgactt
    17641 tcagcgcggt aagcgtgaat tcgtagcggt gtttcctgcc ttcgggagga cacgcgccgc
    17701 cgtagcccgg cgcgccgaag tccgtgcggg tttgcagcgc gcctttgggc ggcctgcctc
    17761 attgtgcggt aatgcctgcg ggcaggcggc ggacatcggc gggaatgtcg gcgaccaccc
    17821 agtgcatccg gtccagaccg atcggcgcgt ctttatcgta aacggtcagg acgaaacttt
    17881 ttgtccctgc gggcgggttt ttccacgaca gcgcgggcga agcattgccg cccgaacagc
    17941 cgaagccgta aggcgcgctc aaaagctggt tttgcgtgaa gccgccgtct tcggacgggt
    18001 tgtcgaattg cagcgtgaat gcgcccccgg caaaggcagc ggttgaaaaa gccagcagta
    18061 cggcggagag gatgcgggtt tgcatagatg attcctttgc ttgatgaata agcggattca
    18121 ttataggctg tcaacggcgg caggaatgtg ccgaaacggt tggatgatgt gccggaatgc
    18181 gcaaatgcaa aaatcgtggt ttcagacgac cctttgaagg ccgtctgaaa tttttcatag
    18241 ggcgacgctt tatgtgtcgt cctgtgtgtt gaaacattcc tttccctgat gctttggaaa
    18301 tattgtatat ttaggcattg ccgcaaccag accctcaagg tttccgccgc agcctgttcc
    18361 tgtaccggcg tgcgggaatg gcggaaatcg gggatttcgc cgtcaaattt atgataggga
    18421 tacggagcaa acgaatgatt accctgcctt ccttaaacaa tctgccgtca aaatgggatg
    18481 aaatccgcca ttgcctcgaa acccgggttt ttgagtgcgg cgttatgccg tatcccaatc
    18541 tgacggaatg cgaagccggg cagttcgagc gggatttttg ggacaatatc ggctgcgcgc
    18601 ctgaagaata tgtccgtatc cgccgcgcca tccgtctgtt ggaattacgt tatcccgaca
    18661 gcttgaacga gctggtgtgt gccgccattg ccacgccttt gggcgagatg ttggcggtgt
    18721 tcggcgttaa gggattgtgc ctgctggagt ttgtcggaca gaagtatttg gaacaggaaa
    18781 ttgccgccgt ccaaaaagcc ttgcgcggac ggtttgtgtt tcgggaagac gggcggatgc
    18841 aatttttgcg tcaggaattg gatttgtatt tccaatgccg tctgaaaacc tttgccacgc
    18901 ccttggaaat gattggtacg gcgtttcaaa aacaggcgtg ggacgtgctt ttggcgattc
    18961 cttacggcga aacgcgcagt tataaggagc aggcgcagcg tttgggcaac cccaaagccg
    19021 tccgcgccgt tgccgccgcc aacgggcaga acaaggtgtc tgtcatgatt ccctgccacc
    19081 gcgtcattgg cagcgacggc aaactgaccg gctacgcggg cgggttgaac cgcaaacagt
    19141 ttttactggc tttggaacgc ggcgaagttc agacggcatt gttttaagcg gggaaatgca
    19201 gaacggctca tcgggcttaa atatggtgtg atgacggctg tgaaaattca tacaacggca
    19261 atccttttaa ggcatcggtc ttaatgtctt tctgattcat atgcttgtcg ttttgtcgcc
    19321 catttttcca aaattaagga ttgacacatt ttttccggac gctataatac gccccattcc
    19381 ccgatagctc agtcggtaga gcgacggact gttaatccgc aggtccctgg ttcgagccca
    19441 ggtcggggag ccagaatttt gaagcccgaa cttgaatgtt cgggcttttt cacatttgca
    19501 cgtccggcgt tcggaatgcg gcgagatagg ttttccaacg gtttcgcggt aaaattctgc
    19561 ctgtctgatt tcggtttcgt tttatgtcta tcaaaatcct gattatttct cccagttgga
    19621 taggtgactg cgtgatgacc cagcccttgt tccgccgttt gaaggaactt catcccggtt
    19681 gcacgattga tgtgttcgca ccgaagtggt cgatggcggt gttcgagcgt atgccggaag
    19741 tgaatgagat tcttgaaaat ccgttcggac acggtgcgtt ggagctgaaa cgccgttggc
    19801 gggtaggcag ggagttggga cggcgcggat acgacagggt tatcgtgttg ccgggttctt
    19861 tgaagtcggc agtcatcgcg ctggcaacgg gcatcggaaa aagaacgggt tatgtcggcg
    19921 aaagccgtta ttttctgttg aacgatatac gcaggctgga taaggaacgt ctgcctttga
    19981 tggtggaccg atatacggct ctcgcgcatc agagtcagga agattttgac gggcattcgg
    20041 gattccccga gttttccatt gatgaacggc ggcgggaaat ttctattgaa actttcggtt
    20101 tgaatcttgg aaagcctgtt ctggcttttt gcccgggtgc ggaattcggg ccggcaaagc
    20161 gttggccggc aaggcatttt gccgagttgg gcaaacacta ttcggaggcg ggttggcagg
    20221 tttggttgtt cggttcgcaa aaagatgatg aaatcgccga agaaatcaac tgcctttcag
    20281 acggcatgtg tgtcaatttg tgcggcaaaa ccgatttgtc gcaggcaatg gatttgctgt
    20341 cgttggcgga cacggtcgtg tgcaacgaca gcgggctgat gcatttggcg gctgctttgg
    20401 gcaggaaggt agtggcggtt tacggttctt ccagcccgac gcatacgccg cctttgagcg
    20461 acagggcgaa aatcgtcagc ctgcacttgg aatgttcgcc ctgtttcaaa cgtgaatgcc
    20521 cgttggggca taccgactgc ctcaacaggc tgtatcccga gaagattgtg caggcggttg
    20581 aagaggcggt atgatggctt ctgttttcgg atttataccg tctgacggcg gatattgata
    20641 ggatgtttat ggctattgcg aacaataaaa aagcatttca cgattttttc attgaagacc
    20701 ggattgaagc cggtttggtc ttggaaggct gggaagtcaa agcaatccgc gccgcgcgcg
    20761 tacagcttaa agagagttat atctattgga aaaaagatgc gttttatctg gtcggctgcc
    20821 atattacggc tttgcctacg gcttcgacac acatcaaacc ggatgccgta cgtccgcgca
    20881 aactgttatt aaaacagtcg gaaatcaaca agttaatcgg aaaaaccgaa cgtgcaggtt
    20941 acaccatcgt gccgctggat ttgcattttt cacgcggaaa aatcaagatg gaaatcggtt
    21001 tggcaaaagg taagaaacag cacgataaac gacaaagtat gaaggaagcc gactggaaac
    21061 gcgagaaaca gcggttgatt aagcatacgc gctgattgtg gaaacaatgc ttcagacggt
    21121 acgggcaagg ctgccgcctg atgcgtaaac gttctacact taaaccagca tccgctttaa
    21181 tcagcacagc atgatgcccc aaaccttcgt caaaggtttg agcaatttga tgataaattg
    21241 gcattagcca agggtaaagt gcggtcattt ttcctgaaat ttttgtaaat aacgagggca
    21301 attatacaca ttttcaaacc ggcttgacaa aatagcaaaa agcaaacgtt tacttttaat
    21361 aaataagaaa taaatagaga aaatttgaac taaatgacat tttttgttca gttttatgct
    21421 aaaatccacg ccctatttat ttttacttga taaaggaaaa tttattatga aactcgaagc
    21481 aagcagaagt ttaaaaaatc atttattata agtctatttt tttctattct ttatacctct
    21541 ccgcttttgg ctgttgatta cgtttacgac aattctaaat taacaaatga gcaaattgat
    21601 cgcttaaaaa aaacttagag ataaaagcag cgaatactgg aaacaagaaa cttatctttt
    21661 aaaaccgacg aatggtagct ctcagaactc tctcaaagtt cccgcactat cctctaaaac
    21721 acaccaattc gaaaacatca acaactcaaa aaagatttct ttttatgaca aggaatacac
    21781 tgaagattac cttgttggct tcgcccgagg tttcggggtt gaaaaaagaa atggggaaga
    21841 agaaaaacca ctccggcaat attttaagga ctgtgtaaat actgagaatt ccaataatga
    21901 taagtgtgaa caaccatcct taggaagcta tgccccgata cgcataaaga gcgatatttt
    21961 cgccttagca tcacaaataa aaaatagcca tatcaacagc gaaattttat ctgtaggtaa
    22021 ttatatcgaa tggcttcgct tcgcccaaca cttaatcagc tttctagttg gcaagagcat
    22081 ctctattctg atgtagatcc attccactat attgaggtta cagacaactc gcacgttatc
    22141 gggcaaacga ttagtttgga tgaattccac ttagaaaatt ctctctggga accccgttgg
    22201 gattctgatg ttggggagtt aaaaacaacg aatgcagaca tccgttttaa tactaaaagt
    22261 gaaagtttac tcgtaaaaga aaattatgct ggcggagctc gttttcgttt tgcttacgat
    22321 ctaaaagata aagtccctga aataccagtt ttaacttttg aaaaaaatat aactggcaca
    22381 tcagatatta tttttgaggg aaaagcgttg gataatttga aacacctaga cgggcatcaa
    22441 attgtcaaag taaatgacac cgcagataaa gatgccttta gactttccag taaataccga
    22501 aagggaattt atacgctctc tttacaacaa cgcccagagg gcttttttac caaagtgcaa
    22561 gaacgcgatg atattgcgat ttatgcacaa caggctcaag ccgccaatac cttattcgcc
    22621 ttgcgtttga acgacaaaaa cagcaatatt tttgaccgca ctttaccacg caaaggcttg
    22681 tggttgcgtg tgattgacgg acattccaac caatgggtgc aaggcaaaac agcacctgta
    22741 gaaggctatc gaaaaggtgt gcaactcggc ggcgaggtgt tcacatggca aaacgaaagt
    22801 aatcaacttt ctattggctt aatgggcgga caagcagaac aacgcagcac tttccgcaat
    22861 ccggatacag acaatcttac aacgggaaat gtgaaaggct ttggtgcggg cgtttacgcc
    22921 acttggcatc agcttcagga caaacagaca ggtgcgtatg ttgatagctg gatgcaatat
    22981 caacgtttcc gccaccgcat taacactgaa tatgctacag aacgttttac ttcaaaaggt
    23041 attactgcct caattgaagc gggtcacaat gcgttattgg cggaacactt cactaaaaag
    23101 ggcaacagcc ttcgtgttta cctacaacca caggcgcaat tgacttattt gggggtaaac
    23161 ggaaaattca gcgatagcga aaatgcccaa gtgaatttgc ttggctcccg ccaattacaa
    23221 agccgagtgg gcgttcaagc taaagcgcaa tttgctttca ctaatggcgt cactttccaa
    23281 ccatttgttg ctgtcaattc aatctaccaa caaaaacctt tcggtgtgga aatagacggg
    23341 gatcgtcgag tgataaacaa taagacggta attgaaactc aattaggtgt agcagcgaaa
    23401 atcaaatctc acttaacctt gcaagcctca ttcaaccgcc aaacaagcaa acatcatcac
    23461 gctaaacaag gtgcgttgaa tttacagtgg acgttttaag ttaaattgta acaaaataaa
    23521 atcggcaggg aaattcactg ccgattttat tattttaaaa gtgcggtcat ttttcgtgta
    23581 atttccacca atttttaccg cactttcaat ttcagcttga acaagttcaa tagtctgctc
    23641 tgcattaatt acgactgctt ttggattatc ttttactaat tctaaatagc gtgctcgagt
    23701 gcgatggaaa aaatctaaat ccatttgctc aatacggtct aactcgccac gtccacgagc
    23761 tcgcgctaaa ccgacaatcg gatctattgt ccttttcttt gttgtgtatg caaatcagaa
    23821 atgccggtta cggaagcagt ttgccaggat tcataatgtt atatggatca aggtgttgtt
    23881 tgatgctttt catcagggca atttcagagg gcgtgcggac agcgggcagc cattgttttt
    23941 tgatgatacc tatgccgtgt tcggcggcaa tcgtgccgtt gcgggcaagg acgttgcgat
    24001 agactgtgct gttgatgtcg ttttcgtaac ggtaaacttc attgctgagg atttcgggcg
    24061 gaaaagtatt gtagtgcagg ctgccgtcgc ccagatgtcc gaagcagacg atttgtatgc
    24121 ctttaaaatt ctgttccaaa tctttggcgc accggcggac aaagtcggca acgcgcccga
    24181 taggaacggc aatatcgtgt ttgatgctgg tgcccagttt gcgttgggag gcggagatgt
    24241 tttcgcgcaa cgcccacata tggatacgtt cttgttcgct ttgcgccaat acgctgtcgg
    24301 taaatccttt tttataaaga aattcgacaa gccgatcatc aagattgctg tcgggtaatg
    24361 agtcggtcaa ctcaagtaaa atatgccatt ctgaatgtgt cggcaggggg agtttgctga
    24421 attcggaaga caactcggcg gcaaaacggc cgatcagttc aaaactgcat aagcgttcgg
    24481 caaagtgcgc ttgggtttcg gtcagcaggc ggacggcgga ttcgatgtcg ggaatgccga
    24541 cccatgccgt tgccttgtcc gagggacggg caaaaagctt gagcgtggcg gcagtgatga
    24601 tgcccagtgt gccttcgctg ccgataaaca gatggcgcag gtcgtagccg gtggtgtttt
    24661 tatgcagggg atggagatgg gaaaccagtt cgccgttggg gaggacgact tccaaaccga
    24721 taaccaggtc gcgcatcgtg ccgtaacgca atacgttcaa acctccggca ttgcaggcga
    24781 tgttgccgcc gatttggcac gagccttcgc tggcgagact gagtgggaac agcctgtttg
    24841 aggcttcggc tgcctgttgg acggtttgga gtacggaacc tgcttcgacg gttatgcagt
    24901 tgtctgacaa attgatgctg cggatgcggt tgagtttgga aaggttcagc aatacgccgt
    24961 tttccgatac tgccgcgccg cacaaaccgg tattgccgcc ttgcggcgta accggaatgc
    25021 ggtgttcgta acaaaaacgc ataatggttt gcacgctttc aacgctgcgc ggctgcaaaa
    25081 tgatgtctgg tgcagacgta aagcggttgc gctgatcttt taaaagcgtc ggagaaggtt
    25141 cggcaatttc atctgccggc aggagtcggg aaaattcggt atgaaggtca ggcatgtttg
    25201 tcggaagggt agggagaaga ttgaggaagg gaatctgatg ccgtctgaaa aatcaaaaaa
    25261 gcaggatact gaacgtatcc tgcttttttg caagcggaaa atgcttattt tgcagcttcg
    25321 gcagcaggag cttcagcggc aggtgcttcg gtagcagcag cttcggcggc ag
//
LOCUS       DAWTCI010000021        24887 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWTCI010000021 DAWTCI010000000
VERSION     DAWTCI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24887
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.021"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(59..253)
                     /locus_tag="WM481_000875"
     CDS             complement(59..253)
                     /locus_tag="WM481_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFA9080224.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMNK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(243..2324)
                     /locus_tag="WM481_000876"
     CDS             complement(243..2324)
                     /locus_tag="WM481_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFA9080225.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMTVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRHIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLNFPVDAATLLHTAKEVGENTILSRTGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVALVPAVGVLLVTCYAGLQKLFHNDPRASFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVTVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEEQSEA"
     gene            2847..3227
                     /locus_tag="WM481_000877"
     CDS             2847..3227
                     /locus_tag="WM481_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFA9080226.1"
                     /translation="MNIRFFALTVSVLSLAACAVPEAYDGGGRGYMPPVQNQAGPDDF
                     RAFSCENGLSVRVRNLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3363..3835)
                     /locus_tag="WM481_000878"
                     /pseudo
     CDS             complement(3363..3835)
                     /locus_tag="WM481_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244189.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
     gene            4183..5571
                     /locus_tag="WM481_000879"
     CDS             4183..5571
                     /locus_tag="WM481_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFA9080227.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIVKP
                     ASLTPLSAYALAVLAYEAGVPQDLLPVVSGRASEISHEFATNPTVRKISFTGSTEVGA
                     KIFAGSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKFRNSGQTCVCTNRVYV
                     QSGIYDVFCRKLSEKAAALKLGDGLDEGVNQGPLIEEKAVEKVEQHIADALSKGAVCL
                     TGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFETEAEVIEAANNTEYG
                     LAAYLFTSDTARQWRVGEVLEYGMVGINTGLISNEAAPFGGVKRSGLGREGSKYGADE
                     YLELKYLCIDAG"
     gene            5682..6467
                     /locus_tag="WM481_000880"
     CDS             5682..6467
                     /locus_tag="WM481_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFA9080228.1"
                     /translation="MMENGKTFPRWRFALKSAGWHLLISLLVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIIP"
     gene            6835..8391
                     /locus_tag="WM481_000881"
     CDS             6835..8391
                     /locus_tag="WM481_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFA9080229.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMVVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESV
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDTDFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8408..9154
                     /gene="surE"
                     /locus_tag="WM481_000882"
     CDS             8408..9154
                     /gene="surE"
                     /locus_tag="WM481_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFA9080230.1"
                     /translation="MNVLISNDDGYLAEGIAILARVASEFANVRVVAPERDRSGVSNS
                     LTLDRPLQLKQAQNGFCYVNGTPTDCIHVGQFALPDFKPDVVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEKALWTLLAHFFKKPPSAPVLWNVN
                     IPAVAPEDVRGIKITRLGRRHHEQNIVPSRNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHTD"
     gene            9256..10269
                     /locus_tag="WM481_000883"
     CDS             9256..10269
                     /locus_tag="WM481_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9080231.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVSSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVE
                     SRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPAPQSPAASPSGTRSVG
                     GIVWQRPTQGKVVADFGGGNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQ
                     HNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSY
                     IAF"
     gene            complement(10667..11149)
                     /locus_tag="WM481_000884"
     CDS             complement(10667..11149)
                     /locus_tag="WM481_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9080232.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRREISLQASGDVSCGC
                     "
     gene            complement(11302..11463)
                     /locus_tag="WM481_000885"
     CDS             complement(11302..11463)
                     /locus_tag="WM481_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080233.1"
                     /translation="MSEQPEKHHASPIEDERKKPVYRMGRAVAGFMLAVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(11537..11998)
                     /gene="recX"
                     /locus_tag="WM481_000886"
     CDS             complement(11537..11998)
                     /gene="recX"
                     /locus_tag="WM481_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFA9080234.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEKTSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAANLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDSC"
     gene            complement(12071..12781)
                     /locus_tag="WM481_000887"
     CDS             complement(12071..12781)
                     /locus_tag="WM481_000887"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFA9080235.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLVIITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDAANMLMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDTTRPDRIIG
                     ALPEIYENLRPQKNKDEE"
     gene            13176..14395
                     /locus_tag="WM481_000888"
                     /pseudo
     CDS             13176..14395
                     /locus_tag="WM481_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
     gene            complement(14455..15261)
                     /locus_tag="WM481_000889"
     CDS             complement(14455..15261)
                     /locus_tag="WM481_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFA9080236.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQ
                     PTFRSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEYKADFPDQSLCQKQANYTTLTFQIGHSHH"
     gene            15453..15662
                     /locus_tag="WM481_000890"
     CDS             15453..15662
                     /locus_tag="WM481_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFA9080237.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVIELLARVLGKNPETTVV
                     VIDEVDTDNWDIGGKSVSERRKEGR"
     gene            15751..16965
                     /locus_tag="WM481_000891"
     CDS             15751..16965
                     /locus_tag="WM481_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFA9080238.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQNKGLRDITVNDVYIGN
                     GVSELIAMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITSKTKAIVVINPNNPTGAVYSKEILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHCHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYHQ"
     gene            complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="WM481_000892"
     CDS             complement(17037..19616)
                     /gene="clpB"
                     /locus_tag="WM481_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFA9080239.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGEILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGADEVAEIVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQNEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIKVEADGDRL
                     KFA"
     gene            19876..20886
                     /gene="trpS"
                     /locus_tag="WM481_000893"
     CDS             19876..20886
                     /gene="trpS"
                     /locus_tag="WM481_000893"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFA9080240.1"
                     /translation="MSKKRVLTGVTTTGTPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEQEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAEFTQMLADGLAWGEAK
                     KLSAAKINAELAELRERYNALTSNPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            20967..21512
                     /locus_tag="WM481_000894"
     CDS             20967..21512
                     /locus_tag="WM481_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080241.1"
                     /translation="MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAVVGKCME
                     TDGKDAPSGWAENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            21829..22173
                     /locus_tag="WM481_000895"
     CDS             21829..22173
                     /locus_tag="WM481_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080242.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAAQAVESDVKDTAASAA
                     ESAASAVEEAKGQVKDAAADAKASAEEAVTEAKDAAAETKEAVSEAAKDTLNKAADAA
                     QEAADKMKDAAK"
     gene            complement(22383..23891)
                     /locus_tag="WM481_000896"
     CDS             complement(22383..23891)
                     /locus_tag="WM481_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFA9080243.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSTSSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPCGGKMLVIDIGGSSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMGGIISGNEMLWYAVLSLRLAALFCRSRQDLGFP
                     KNMQLRTDTESCGFILRIDRKWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(24054..24785)
                     /locus_tag="WM481_000897"
     CDS             complement(24054..24785)
                     /locus_tag="WM481_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9080244.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSRRLGERWRLTLNAGNMWKHYQEDRT
                     AARYGSHMPLAGATLMYSAPKDRLLYGGADWPHDMTKEAEQASVRKGLRVGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSLRHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         5691 a         6270 c         6898 g         6028 t
ORIGIN      
        1 ccttattata aaaataccgt ctgaagtggc gttcagacgg catttgtttc aagcggggct
       61 aacagcagcg tccgccgttt gcgccgcagc ggcgtttgcg gcaatagtct tgaaattgca
      121 gcttgttcat cacgggggcg ttgggattat gtttacgttg ttgtgcaacg tagttttcat
      181 aatcgggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac caagacgcga
      241 gcttatgctt cactttgttc ctccggttgt ttgccgtcgc ggtacaccgc cgggatttct
      301 ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc gtacgcggca
      361 acagtcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt gtcgttgaag
      421 atgatttgcg ccatttcgcc gatgtcttta gcgggcgcaa ggacttcgtt tttagccaat
      481 gcgtcgctgt atttgcctgc gtgggcaagg aagctggcgc gcgggtcgtt gtggaacagt
      541 ttttgcagac cggcgtagca ggttacaagc agtacgccga cggcaggaac gagtgccacc
      601 cagacataac ggtcgcgttt catcttaatc agcacaacgg cacacataat caaggctacg
      661 cctgccagca tttggttggc gatgccgaat aaaggccaga gcgagttgat gccgcccagc
      721 gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa gaaggtcgca
      781 atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat gctgcccaag
      841 tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag gatgaacaag
      901 gcttcaaaca acagggcgaa gtgataccag aatgccatca tcgcctcgcc cggaatcaga
      961 cggctcataa tgtgcgccat accgactgcg agggtaggcg caccgcctgt gcgggacagg
     1021 atggtgtttt cgcccacttc cttagcggtg tgcaacaggg ttgcggcatc gacagggaag
     1081 ttcagcttgg tggtaattac ttcggcggcg gtgttggcat ccgtaccgat cagtgcggcg
     1141 gggctgttca tcgcgaaata cacgccggga tcgagcgatg cggcggcggc aagcgccata
     1201 atcgccacga aactttccat caacataccg ccgtaaccga tcatacggac gtgggtttcg
     1261 ttttccagca ttttcggcgt ggtgccggaa gaaatcagtg cgtggaagcc cgaaaccgca
     1321 ccgcaggcga tggtaatgaa caagaacggg aacaatgcgc ctgagaatac cgggcccgaa
     1381 ccgtcgataa agtgggtaac cgcaggcatt tgcaaagcgg gattgacgat gacgataccc
     1441 aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc gcgcggggtc
     1501 agcaagagcc agaccggcaa aaccgaagcc acgaaaccgt aaatcataat cgcccaagtg
     1561 agctggatgc cgtcaaggtc aaaccaatgc ccgatggagc ttttcgccac atcttcgccg
     1621 taaattaccg ccaacatcag caaaataaag ccgacgatgg aaatctcgcc gattttgccc
     1681 ggacggatat ggcgcgtgta aatacccata aacagcgcaa tcggcatcgt tgcggcaatg
     1741 gtgaacgtac cccaagggct gtgaaccaat gcttttacga caatcaacgc caaaaccgcc
     1801 ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg gacagtgccg
     1861 agttcctgtt tcacaatatc gcccaaagac ttaccgtcgc ggcgcataga gacgaacaag
     1921 accgtcatat cctgtaccgc gccggcaaat accacgccga agataatcca caaagtaccg
     1981 ggtagataac ccatttgcgc cgccaaaacc ggaccgacca aagggcccgc gccggcaatc
     2041 gcggcaaagt ggtgtccgaa caatacgcct ttgtgcgtcg gaacgtagtc caagccgtcg
     2101 ttgtgccgct cggcgggcgt gagacggttc ggatcgagcc gcatcacgcg gttggcgatg
     2161 tagaggctgt aaaaacggta ggcgatacag taaacggata tggcggcggt aaccatccat
     2221 accgcgctga cctgttcgcc tcggctgagg gccagagtgg taaaggatgc taagccgacc
     2281 agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata aatccttatc
     2341 tcacactgtc ggaatatgtc ggagcggaca ggcaacggtt ggctacccgt aatccgatgc
     2401 cgacacaaac cggagtttga atgattgacg aagtatgaaa cagtgcgctt gtcgggactt
     2461 gtgccttatg cccgcatcaa acagtgcagg aatgtacgcg cacaaacgtt aaacgccgaa
     2521 tagaatttta acgcaaatta gcacactgat agcggttttt acttggaatt tggaaaaatt
     2581 tacattcctc cgggcgggca ggcaggttca gacggcatcg tcaggcaaaa ggcggtgtcg
     2641 gaagacgggt aaagaagggg cgcgcaatcc ggattattga tttatcgtgt ttccttttcg
     2701 gttgaacctc agccctttgg ggcggtaaaa tcagacttta tttgggaggg gcgcaacccc
     2761 ttccgaatca ggacggcaca tagggcggtg ctttaatgtg tcgtcctgtg tgttgaaaca
     2821 tattttcttt ttattgggga gcttttatga atatcaggtt tttcgcgctg accgtatcgg
     2881 ttttgtcttt ggcggcctgt gccgtgccgg aagcgtatga tggcggcgga cgcgggtata
     2941 tgccgcctgt tcaaaaccaa gccggcccgg acgattttcg agcgttttca tgcgagaacg
     3001 gtttgtctgt gcgcgtccgc aatttggacg gcggcaaaat cgcgttgcgg ctggacggca
     3061 ggcgtgccgt cctctcttcc gacgttgccg catccggcga acgctatacc gccgaacacg
     3121 gtttgttcgg aaacggaacc gagtggcacc agaaaggcgg cgaagccttt ttcggcttta
     3181 ccgatgccta cggcaattcg gtcgaaactt cctgccgcgc ccgttaaacg gggcagcctg
     3241 tttgaaccgc cgtccggatt tttcgggcgg ttttttatgc cggttttgtt ttggccgtcg
     3301 gctggttccg gcggttttcg ggcgtggcgg attatttttc gtttcgttct gggtttatcc
     3361 tgtcggcagc ggctcaatgc caactttaaa cctgctccga tttcttcagg gctgttatcc
     3421 aatgataaaa ttacatcgtc tgcatcaatg gcatcccacg cttccggctt gacatggcgg
     3481 ctcgggctga ttttcaggca gccgttgtgc agccaaatat ccccgctcat catgttttta
     3541 aatagggcgc gtctggtttt atagcccaag ttcccgcata ggttggcaac ccaatcctca
     3601 tagcgttgcc gaaatttttc ggtatcaaaa aaatcttggt cttccgaact gtcataaacg
     3661 aaagtcctgc tgtttgccaa cgcttgcaag accgtcgtgc ctaaagtttc attgtcggta
     3721 tccaatggca ggatatgggg ggatataggt ggtctattgc cgttaacccc aaacctgaac
     3781 atgtttgaac aatcagagct tcttcattag cataaaaaac tgcccaataa ttgatcctgt
     3841 ttaaaaatca ttatttaatc tccgtaattt tgactgtaat gttttgactt ttgccatact
     3901 ctaccacgcg ttgtaactgc aatctttgct tcttatatat tgtaccctat caaagggctg
     3961 cattactttt ctttctaaag attactatcg cataaaaagt atttttctta aacgacaggg
     4021 atgcgcccgt gccatattca aacaccgccc ggatgttgcg ctgcccgatc ggatgcttca
     4081 gacggcatcg gaagggtttg cagttttgga atatgaagat gataatgtcc gcgagattga
     4141 cggcatttga aagcggttat cccgtatacc ggaggatacg aaatgaacga atattcccaa
     4201 ttaatcaagc atcccgatat ttccctttcc ccgatttcag acggcatcgg ggtcggcaat
     4261 ccggcgacgg gcgaaatttt ggcgtatgtc cgcaacacgg gttcggacaa gctgaaaaac
     4321 ctgattcaaa aggcggcggc agcgcaaaaa ttatgggcgg cgaaaaccgc cttggaacgc
     4381 gccgacatat tgtggcgttg gtatttttta gttaaagaaa acaaagaagc actggcgcgt
     4441 ctcatgacga tggagcaggg caaaagcctg accgaggcgc gcggcgaaat cgattatgcg
     4501 gcttcgtttg tgcgctggtt tgccgaagag gcgcggcgga ttgacggcga tgtgctgacg
     4561 agtgtgaaag cgtcgcaaaa actggttgtg ttgaaacagc ccgtcggcgt taccgctgcg
     4621 attacgccgt ggaatttccc gtccgcgatg atagtcaaac ctgcatcgct cacgcctttg
     4681 agtgcgtatg cgttggctgt actggcctac gaagcgggcg tgccgcagga tttgcttccc
     4741 gtcgtcagcg gccgcgcttc ggaaatcagc catgaatttg ccacgaaccc gaccgtgcgc
     4801 aaaatcagct tcaccggctc gaccgaagtc ggcgcgaaaa tttttgccgg cagcgcggcg
     4861 gacattaaaa aactcagttt ggaactgggc ggcaacgcgc cgtttatcgt gtttgacgat
     4921 gccgatttgg acaaagccgt cgaaggcgcg ctggcgagca agttccgcaa cagcggtcag
     4981 acctgcgtct gcaccaaccg cgtttacgtc caatcgggca tttacgacgt attttgccgc
     5041 aaattgagtg aaaaggcagc cgcgctcaaa ttgggcgacg gcttggacga aggtgtgaac
     5101 caaggaccgc tgattgagga aaaagcggta gaaaaagtcg aacagcacat cgccgacgca
     5161 ctctccaaag gcgcggtctg tctgaccggc ggcaaacgca gcgcgttggg cggaacgttt
     5221 tttgagccga ctgtcttaag cggcgttacg gcgcaaatgg cggtagctcg cgaggaaacc
     5281 ttcgggcctt tgtgtccggt gttccgtttt gaaaccgaag ccgaagtcat cgaggctgcg
     5341 aacaatacgg aatacggttt ggcagcttac cttttcacct ccgacaccgc ccgtcaatgg
     5401 cgcgtcggcg aagtcttgga atacggcatg gtcggcatca atacgggctt aatcagtaat
     5461 gaagcggcac cgttcggcgg ggtgaaacgc agcggtttgg gacgtgaagg cagcaaatac
     5521 ggtgcggacg aatatctaga attgaaatat ttgtgcattg atgccgggtg acggatgccg
     5581 tctgaatggc agggtttcag acggtgtcgc attttaagca gtctctatct gttgtacaat
     5641 gcgccctgtt tttacggtta tttttgattt gaatgtaaga tatgatggaa aacggaaaaa
     5701 cattcccgag atggcgtttt gccttgaaaa gtgcgggctg gcacctctta atcagcttat
     5761 tggttgcagg gctggcggcc ttgctggttt ttaaggtttg gtatccttat ccttatgccg
     5821 agctgacggg cggtctgtcg ctttatcagc tggtggtggc ggtcgatatc gtatgcggcc
     5881 cgctgctgac tttaattttg gcaagcccga agaaaaagac aaaggcacgc atggtcgatt
     5941 tttccatggt cggcatcatc cagctggcgg ctttggcgta cggtttgcac agcgtttcgc
     6001 tggcgcgtcc cgtggtggaa gcgtttgaac aggaccgcat gaccattgtt acggcggcgg
     6061 aagtcgtggt cgaagatttg cacaaagccc ccgaagggct gcaaagcctg tcgtggttcg
     6121 gcatccgccg cattgcattg aaagaacctg aggatgcgga tgagaagaac aagacgctgg
     6181 atttgtccct gaaaggtatc gagccgagta tgcgtcccga ccggtggctg ccgtattccg
     6241 acaaggaagc agaaaaaatc cgcaaacatc tgaaaccgct gaaagtcttg gcggatgcga
     6301 gaaaaacgac ggttgcggac attctgaaac aggcaggtct cgccgaaggg gaggagctgt
     6361 attacctgcc gtttaccagc agcaggcaga aagagtggat agtcattacc gataaagagg
     6421 gcaacaccaa aggctacgcg ccgatagacg gcttcatcat cccttaacca ttgggattcc
     6481 gcccgcactc gaacatccgt tcttcgcggc ggtagaatca gactgtattt gagaggggcg
     6541 caacccctca atcagggcaa cacataggga gacgctttat gtgccgtccg tgtattgaaa
     6601 catttcaaaa taagcccatg ccgtccgaac atcctttcag acggcatggc gttttgccat
     6661 tgccccgata agctgttaaa ctgtttaaat tatttcgacc gaaggtacac gcacatgaaa
     6721 gaccccgagc agagcagtaa gcccgcccgc cgttttttgt gcgtaccgtc tgttcaggca
     6781 gcttgagcgt ttcaggctgc ctttttgcgt ttgaacttga ggaacacgaa aatcatggat
     6841 ttcagttggt tggcagaacc gcatacctgg ataggttttg ccacgctttt ggtgttggaa
     6901 gtcgtattgg ggatagacaa ccttgtcttt gtggcgattt tggcaaacaa ggtccagccc
     6961 gcacggcgcg accgcgcacg gattatcggg ctggggctgg cagtcgtcat ccgcatcatt
     7021 atgcttgctt ttatggcgca catcatcacg ctgaccgagc cgctgttcca aatcggcggc
     7081 ctcgccgttt ccggcaagga catgattatg ctcgcgggcg gtattttcct gctttacaaa
     7141 gccaccaccg aactgcatga acgcctcgaa gggcacaacc gttttaccgt tgccgacaac
     7201 caaaaaaaac acgcgccgtt ttggggcgtg gtcgcgcaaa tcctgatact ggatgccgtg
     7261 ttttccatcg attcggtcat caccgccgtg gcgatggtcg atcacatcgt cgtggcgatg
     7321 ggcgcggtcg tcgtcgcaat ggttgtcatg atttccgcca gcaaactctt gaccgaattt
     7381 gtggacaggc atcccaccgt cgtgatgctc tgccttggtt ttttgttgat gatcggtttc
     7441 agcctgattg cggaagcctt ccatttccac attcccaaag gctacctcta cgccgccatc
     7501 ggcttctcga ttttaatcga actgtttaac cagatttcgc aacgcaacag ccgcaaaaac
     7561 gactacatcg gcagctcgtg gcgcaagcgc accgccgaaa acgttttggg tatgatgggc
     7621 atacgcgaaa gcgtgcttgc tgacgcgggc ggcgaatccg tggatgacgc gcattttgaa
     7681 gaaaacgaaa aatcgatgat acgcagcgtg ctgacgcttg ccgaacgccc gattatgggg
     7741 gtgatgattc cacgccgcga catcgaacgg ctggacattt cccaaagccg cgaagaacag
     7801 tatgcccaac tgcaaaacac gccttacagc cgcctgctcg ttgtcggaaa ggcgggcgtg
     7861 gacgaaccgt tgggctacat caacaaaaaa gacctgctgt cccaactgct ggaaacaggc
     7921 ggtctcgaca ttcagacggc attgcgccag ccgctcgtcc tgcccgacgg cacgaccgcc
     7981 ttgggcgcgc tcgaactctt ccgccaaagc agcgcggatt acgctttggt ggtggacgag
     8041 ttcggcgcgg tgttgggtat ggtgacgatg aaagacctgt tggaagccat cgcaggcgag
     8101 ttccccgaag aattcgagcg cgaagaagaa cccgccgttc aggggaatcc cgatgaaagc
     8161 ctgacggtgg aaggcgcgtt ggaatatgtg gagctggcat cccaactcaa cctgccgcag
     8221 caggaggaag ataccgattt ccataccgtt gccgggctga ttatggaaga attgcaaacc
     8281 atccccgatg tcggcgattt tgccgatttt cacggctggc ggtttgaagt ggtcgaaaaa
     8341 gaagggcagc gcatcgagcg ggtcaaaatc accaaattgc ccgaagaata agcatccagg
     8401 ataaaaaatg aacgttttga tttccaacga cgacggctat ctggcagaag gcattgccat
     8461 tctggcgcgg gttgcgtcgg aatttgccaa tgtcagggtg gtcgcgcccg agcgggacag
     8521 gagcggggtc agcaattcgc tgacgctcga ccgtcctttg cagctgaaac aggcgcaaaa
     8581 cggcttctgc tacgtcaacg gcacgccgac cgactgcatc catgtcggcc agtttgccct
     8641 gcccgatttt aagcccgatg tcgtgttttc aggtatcaac cgtggcgcaa atatgggcga
     8701 cgatacgctt tattcgggga cggttgcggc ggcgaccgaa gcctatctga tgggtatgcc
     8761 tgctgtggcg ttttctttaa acgatgcttc cggacgctat tgggcaaccg cagaaaaggc
     8821 actgtggaca ttgttggcgc atttttttaa aaaaccgccg tccgcgcccg tcttgtggaa
     8881 tgtcaacatc cctgctgtcg caccggaaga tgtcagggga atcaagatta cccgtttggg
     8941 cagacggcat catgagcaga acatcgtccc gtcccgcaat ccgcgcggcg aacagattta
     9001 ttggataggg ccggtcggcg aagtttccga tcgggaagag gggacggatt ttggtgaatg
     9061 cggcgcaggt tttattaccg taacgccgct gcaaatcgat ttgaccgcct atccggacat
     9121 ggcggagacg gcggcctttt ggcatacgga ctgaccgttt ttcaaatacc gtcgggtttt
     9181 gcgcttgaga agaacgcgca tttttattat gatataccgc attcaggcta tatatcggaa
     9241 catgaaggtt atcttatgtt gaaacaaacg acacttttgg cagcttgtac cgccgttgcc
     9301 gctctgttgg gcggttgcgc cacccaacag cctgctcctg tcattgcagg caattcaggt
     9361 atgcagaccg tatcgtctgc gccggtttac aatccttatg gcgcaacgcc gtacaatgcc
     9421 gctcctgccg ccaacgatgc gccgtatgtg ccgcccgtgc aaactgcgcc ggtttattcg
     9481 cctcctgctt atgttccgcc gtctgcacct gccgtttcgg gtacatatgt tccttcttac
     9541 gcacccgtcg acatcaacgc ggcgacgcat actattgtgc gtggcgacac ggtgtacaac
     9601 atttccaaac gctaccatat ctctcaagac gatttccgtg cgtggaacgg catgaccgac
     9661 aatacgttga gcatcggtca gattgttaaa gtcaaaccgg caggatatgc cgcaccgaaa
     9721 accgcagccg tagaaagcag gcccgccgta ccggctgccg cgcaaacccc tgtgaaaccc
     9781 gccgcgcaac cgcccgttca gtccgcgccg caacctgccg cgcccgctgc ggaaaataaa
     9841 gcggttcccg cccccgcgcc cgccccgcaa tctcctgccg cttcgccttc cggcacgcgt
     9901 tcggtcggcg gcattgtttg gcagcgtccg acccaaggta aagtggttgc cgatttcggc
     9961 ggcggcaaca agggtgtcga tattgccggc aatgccggac aacccgtttt ggcggcggct
    10021 gacggcaaag tggtttatgc cggttcaggt ttgaggggat acggaaactt ggtcatcatc
    10081 cagcacaatt cctctttcct gaccgcgtac gggcacaacc aaaaattgct ggtcggcgaa
    10141 ggtcagcagg taaaacgcgg tcagcaggtt gctttgatgg gcaataccga tgcttccaga
    10201 acgcagcttc atttcgaggt gcgtcaaaac ggcaaaccgg ttaacccgaa cagctatatc
    10261 gcgttctgac tttccgtttg caatcaggtt aaaaatgccg tctgaaaggt cttcagacgg
    10321 catttttgtt ttggcggctg tgttttcaaa tcattccgaa gggacggggc gtataaattt
    10381 gtttgactgt gtttgggtat ttgcttgatt tcatccgttt gcaatggaaa agccgcttat
    10441 ttccgttcgg agggcggttt tatcggataa aaggcatttt gtccgactga ttagggctgc
    10501 atcaagggaa tatgaaaaca caaccgctta gtctcacggc tgtgttccaa aatggcgttg
    10561 aaaaacggat gagccagaat cagccgattt tacttcaata aatcaaacca ttacaccgaa
    10621 tgtcggaaca atgcttgtcc tgtctttcag acggcatagg ccgccgtcag cagccgcagg
    10681 acacgtctcc ggaggcttgg aggctgattt ctctgcgttt tttggctttt tcgtagcggc
    10741 agcgttggcg gtcggtcagg atttccagcg gagggacggg gacgggtttg ccgtctttga
    10801 ccgcaaccat cgtgaagtag cagctgttgg tatggcggat ttcgccggtg cggatgtttt
    10861 gtgcttcgac acggatgccg atttccatcg aggtgcgtcc cgtgtagttg acagcggcgt
    10921 aaaaggtaac caaatcgccg atgtggatgg gttctttaaa caggactttg tcaaccgaaa
    10981 gggtaacgca ataattgccg ctgtaacggc tggcgcagga ataggcaacc tggtcgagca
    11041 ggagcaggag ttcgccgccg tgtacgttgc cgctgaaatt ggcggtgtcc ggcatcatca
    11101 gttcggacat aatgagttcg tgggaaggca gttggcgttg ttgtgtcatg gtgtacctgt
    11161 tttaagaaag ggtgttttga aatgggtgcg ggaaaatttt agggaatggg gaagtggaat
    11221 tcaagctaaa aaaagctatg gagggatagt ctgccgaaat gggccgccga tgccgtctga
    11281 agccttcaga cggcattttg ttcaggaaag ccaaaaacgg aaaaccaaga aaaacacgag
    11341 tgccagcacg cccgcccaaa cggcgagcat gaatccggca accgcccgtc ccatacggta
    11401 aaccggtttt ttgcgttcgt cttcgatggg ggaggcgtgg tgtttttcgg gttgttcgga
    11461 cattttgttc tcaatcggaa aagttggaaa cgggcgtaag gttacgctgc cgtgcaaaaa
    11521 gatttaagaa ggaaggtcag cagctgtctt cccaattttc gtcccaagcg tgtttcaatg
    11581 ccgtctgaac ggtatcggca tcaaaaccgc gataggcgag gaaacgcgcc tgtttttgtt
    11641 tttctttgag gttggcggcg gggtgtttga attttttacg caacacggct atggcggctt
    11701 gcttttcgct tgagcggtcg ggaagcaggt tgcggctggt tttttcatct atgccctgtt
    11761 gcgccaaagc ctgtttcagc ctcaatgaac cgtgtttgcg gcttttgctg cggatatagg
    11821 cttcggcgta gcgcaaatcc gactgccagt tgcgttcggc aaattcgttt aacacgtttt
    11881 ccaactcctc ttcgctttcg gcgtgcggtg caagtttgcg tttcagaccg atgcggctga
    11941 cttcttggcg cgagaggatg tccatcgcgc gggcgcgtag ggatttttgc ggtttcatgg
    12001 cggcggtttc ggcacggaaa gattcagacg gtatgggaaa cggattccga tgccgtctga
    12061 acagattgtt ttattcttcg tctttgtttt tctgcggtcg caggttttcg taaatttcgg
    12121 gcagcgcgcc gataatccgg tcggggcggg tcgtatcgtc ttgcgagagc agcgtcatat
    12181 cgccgtaacc gaaggtaacg ccgacactca ggcagccggc ggctttggcg gcgatgatgt
    12241 cgttgcgcga gtcgccgacc ataagcatat tggcggcatc gatacccaaa acttcggcgg
    12301 cgtgccgcag cggcagcggg ctgggttttt tttcgggcag gctgtctccg ccgagtatca
    12361 ggctgaagta gtcggcgagt cccagttgtt ttagaagctc ggcggcaagg atttcgtttt
    12421 tgttggtaat gattaccagc ggaatgccca aggatttgag caatgccagc ccggcttcgg
    12481 tttcgggata ggggcgggtg aagacgctca aatggtcgcg gtagtatttc atataggaca
    12541 cgaaaccttt ttcccacagt tcggaatcgg cttcgcggtc gcggtcgttg gtgaggacgc
    12601 ggtgaaccag tttgccgatg ccgtcgccga cgtagctttc gaccactttg gcgggcagcg
    12661 gtttcatacc gagttgttcc aacatcgctt ctgcggcggc ggcaaggtcg gggacggaat
    12721 cgcacagtgt gccgtccaaa tcgaaggcga cggcttggac gtgttcgatg gctgcattca
    12781 taatccggtt tcctgtcggg ttgaaagggt ttattttaac atcgtttgca cagggcttct
    12841 aatgtataga ttattaacaa tcgcaacagg tttttcgggc ggtgttttcg gattttatgg
    12901 cggggtctgg caagggtttg aaatcccggc gatgttccaa ttaaattttt aaaaaataaa
    12961 atgatgacta tatctttatt taattggaca tttgcttttc ggatgcgcgc tgcttttttc
    13021 agacggatgc tgtgcggctt gaaaaataaa atcatatata gattaataaa atcgggctta
    13081 tgtcgactac atgctattac gttttgtcgg gaaattggtt atagtaatga gcgaaatgca
    13141 cgcccctgaa aatctttttg cgaaaaggaa gcaaaatgtc tgatgccgtt gcaaaagaaa
    13201 ccctcaatcc gtttgaaatc gcgcgaaaac aggtcaaaac cgcctgcgac cggctgaaaa
    13261 ccgatccggc cgtttatgaa atcctgaaga gtccgacccg cgtgttggaa gtcaaactcg
    13321 acgacggcac ggtcaaaacc tttaccggtt accgttcgcg tgcgttccca tcccggtgtg
    13381 aatctggacg aagtcaaggc actgtcgatt tggatgacca tcaaatgctg cgttgcaggc
    13441 attccttacg gcggcggcaa aggcggcgtt accttggatc cgcgcgatta ttccgaggcg
    13501 gaactggaac gcatcgcccg agcttatgcc gaagcgattg ccccgctgat cggcgaaaaa
    13561 aatcgatatt cccgcccccg atgtgaacac caacggcaaa attatgtcgt ggatggtgga
    13621 tgcctatgaa aacgtggtga aacattccgc gccgggcgta tttaccggca aaccggttga
    13681 gttcggcggc tctttggcgc gtaccgaggc taccggttac ggcgtgaacc tcgccgccgt
    13741 ccaagctttg gaaaaactgg gcaaagatgt gaaaggcgca acctacgcca ttcaaggttt
    13801 cggcaatgtg ggctaccaca ccggctatta cgcgcatcaa tccggcgcga aagtcgttac
    13861 cgtatctact gttgacgttg ctatctacaa tgaaaacggc ttggatatgg aagcgctgtt
    13921 taaagagttc caagaaaaag gcttcatcac caacaaagcc ggttacggca aagaaattac
    13981 caacgccgaa cttttggctt tggatatgga cgtactcgcc ccttgcgcat tggaaaacca
    14041 gctgacttcc gaaaacgccg gtaaagtccg cgcgaaaatc gtggttgaag gcgcaaacgg
    14101 cccgactacg cccgaagccg acgttatcct gcgtcaaaac ggcgtattgg tcgtgcccga
    14161 tattttggca aactgcggcg gcgtggtcgt ttcctatttc gagtgggtgc aaaacctgca
    14221 aggctattat tgggagtttg acgaagttca ggaaaaagaa atcgtcgtcc tgcgccgcgc
    14281 gttccgcgat atttggaact tggcgcaaga gtatgatgtc gatttgcgta ccgcgtctta
    14341 tatgatgagt atccgccgtg ttgaaaaagc gatgaagctg cgcggctggt attgatgttt
    14401 gaacaaagga tgccgtctga aaccatttca gacggcattt ttgtttggac ggaattaatg
    14461 gtgagaatgg ccgatttgga aggttaaagt tgtgtagttc gcctgttttt ggcacaggct
    14521 ttgatcgggg aaatcggctt tgtattcgac actcgctttc caaaagcctt ggcgcaaggg
    14581 gatgatgtcc acttcgcctt tgccgtcggt ggtgtcggag aaggcttggg cttcggtttt
    14641 gtgcgttttg ctgcggtcgc tggtgtcgaa gccgtcaaat gtagcggtaa cggtggcatt
    14701 gggcagcggt tcgccacgga acagaacgcg gactttgaag cgttcgccta cgtgaatgtt
    14761 ggcgggattg tccagcggga cgatttccaa gttttgtccg accggtttgg tgatgatggc
    14821 ggtgtccgcg ctttcgtgtc ccacgttgac aatgttttta ccgaacatac gggtttgttc
    14881 gcaatagctt gcgtcaggca tttctttgat gccagcctgt ttccagcctg ctttgttttt
    14941 tgaccggaaa gtaggctgat attcggcggt aacgaggtag ctgccgtctt tgacgggacg
    15001 gttgctgcgg tattggtagt tgtatgtgcc gcgttgaatc atgttttcct tacctttttc
    15061 ggtaaccagc tgcatcggtt tgctgaagat gtgcaggcgg tctttggcga tgggttcgag
    15121 ttcggggaat tcgccgtagc ccaagtcggc tttaaggtat tcgccgccgt gcgtgtgggc
    15181 ggtttcgacc cagacgcggt gggcgtgtgc ggatgtggca aatagtgcgg aaacggcgag
    15241 cagtgtcaat gttttcttca tggtgcagcc tttatatcgt gcgggttaaa aattttgtta
    15301 tgctataaca aaatatgtat cgtgtgaaga cggatttgcc gcctgaaaac tgtctttttg
    15361 tatcgatttg aatttttcag aaaatgaagc ggacgtttgg gcggaggcaa cttgtttgat
    15421 aagatagcaa tatttcaaaa cggagaaata tcatgcctta tgtcaatatt aaagtaaccg
    15481 gcggcaagga agcaccgact gccgcgcaaa aagcggaact gatcggcggc gtaatcgaat
    15541 tgctggcacg cgtgctgggt aaaaatcccg aaacaacggt tgtcgtgatt gacgaagtgg
    15601 ataccgataa ctgggatata ggcggcaaaa gcgtcagcga acggcgcaaa gagggcaggt
    15661 aaaaagcctg aaaaccccgc cctgatgctt taaatttcgc gtgaaaaaga gtacattccc
    15721 acccattgcc caaaatttac ggaacacatt atggataaat ttcccaagtc tgcaaagctc
    15781 gaccacgtct gctacgacat acgcgggccg gttcacaaaa aagcccttca gttggaagag
    15841 gagggcaaca aaatcctcaa gctcaatatc ggcaaccctg cgccgttcgg ctttgaagca
    15901 cctgatgaaa tcttagtcga tgtcatccgc aacctgccga cttcgcaagg ctattgcgat
    15961 tcgaaggggt tgtattccgc acgcaaagcc attgttcact actatcagaa caaaggtttg
    16021 cgcgatatta cggtaaacga tgtctatatc ggcaacggcg tgtccgagct gattgcgatg
    16081 tctatgcagg cgttgctcaa cgacggcgac gaaatcctga ttcccgcgcc cgactaccct
    16141 ttgtggacgg cggcggcaac gcttgcgggc ggtacggtac gccattatct gtgcgacgaa
    16201 gaaaacggct ggtttcccaa ccttgccgat atggaagcca aaatcacgtc caaaaccaaa
    16261 gccatcgtcg tcatcaaccc caataacccg acaggagcgg tgtacagcaa ggaaatcctg
    16321 ctggaaattg ccgaactggc gcgcaagcat ggtttgatta ttttcgccga cgagatttac
    16381 gacaaaatcc tttatgatgg cgcggttcac tgccacatcg ccgcgctcgc ccccgacctt
    16441 ttgacggtaa cgttcaacgg tttgtccaaa gcctaccgtg tagccgggtt ccgccaaggc
    16501 tggatggtgc taaacgggcc gaaacatcac gcaaaaggtt atatcgaggg tttggatatg
    16561 ctctcgtcca tgcgcttgtg tgccaatacg ccgatgcagc acgcgattca gacggcattg
    16621 ggcggttatc agagcattaa cgaattcatc ttgcccggcg gacggctttt ggaacagcgc
    16681 aacagagcgt gggaactggt caaccagatt cccggcgtgt cctgcgtcaa accgatgggc
    16741 gcgatgtata tgttcccgaa aatcgatacc gaaatgtacc gcatccgcga tgatatgaaa
    16801 ttcgtttacg atttgctggt gcgcgaaaaa gtcctgctgg tacagggaac ggggtttaat
    16861 tggatcaaac ccgaccattt ccgcattgtt acgctgcctt acgtccatca gattgaagag
    16921 gcgatgggca ggttggcaag attcctgcaa acctaccatc agtaggattc tgataaaaaa
    16981 tgccgtctgg aacggagatt cccgtttcag acggcatttt caacagcagg aacaaatcag
    17041 gcaaatttca gtctgtcgcc gtcggcttct accttgattt cgctttcggg cgcatagttt
    17101 ccggcaagca gggctttggc cagcgggttt tcgatttccg attggatcgc gcgtttgagc
    17161 ggacgtgcgc cgtaaatcgg gtcgaaaccg gctttggcga tgatgtccag agcggcatcg
    17221 gaaacagtca ggcgcaggtt ttgtttttcc aaacgttttt ccaaaccttt gagctggatt
    17281 ttcgcaatac tgcggatatt ggcctgatcc aagccatgga acacgaccac ttcgtcgatg
    17341 cggttgatca tttccggacg gaaatgttct ttcacatcct ccatcacaac ttctttcacc
    17401 gcttcgtagt cctgcgtgcc catttgttgg atgtgctggc tgccgatgtt ggaagtcatc
    17461 acaatcacgg tatttttaaa atctaccgtc ctgccttgtc cgtcggtcaa acgaccgtca
    17521 tccaatactt gcagcaggat gttgaatacg tcgggatggg ctttttccac ttcgtccaaa
    17581 agaatcacgc tgtacggttt gcggcggact tgttcggtca ggtagccgcc ctcttcgtat
    17641 ccgacataac ccggaggcgc gccgattaag cgggcaacgg cgtgtttttc catgtattcg
    17701 gacatgtcga tgcggatgag gtggtcttcg ctgtcgaaca ggaagcctgc caaagctttg
    17761 cacaactcgg tcttacccac accggtcggg cctaagaaca ggaagctgcc gtaaggtttg
    17821 ttcggatcgg caaggccgga gcggctgcgg cggatggcat cggacacggc gcgcacggct
    17881 tcgttttgac cgactacgcg gcggtgcaac acttcttcca ttttcaacaa tttatcgcgt
    17941 tcgccttcca tcattttgga tacgggaatg ccggtcatac gggaaacgat ttcggccact
    18001 tcatccgcgc cgacattatt acgcaagagt ttgtttgccg gttttgtgct gtccgtatct
    18061 gcccgttcgg cggctgcacg ctgtttttcc aaatgctcca aatcttcata catcaatttt
    18121 gaagccagtg ccaaatcgcc ttgccgtttt gcctgttcga ttttaatttt gacttcgtca
    18181 atttgtttct taatattggc agcaccgtct gaaatcgctt tttcggcttt ccagatttcg
    18241 tccaaatcgg cgtattcttt ttgcaagccg tcgatttcct cgtctatcag ttccaaacgt
    18301 tttttgctgg catcgtcttt ttctttttca acgtgcgcct tttccatccg aagctgaatc
    18361 aaacggcggt cgattttgtc catagcttcc ggtttggttt ctttttccat cttgacacgg
    18421 ctggcagctt cgtcaatcaa atcaatcgcc ttgtcgggca aaaagcggtc ggtaatgtag
    18481 cggtcgctca actccgctgc ggcaacaatg gcggggtcgg taatatcgat gccgtggtgg
    18541 atttcatagc gttcctgcaa accgcgcagg atagcgatgg tgtcttccac gctgggttcg
    18601 ccgaccaata ctttttggaa acggcgttcg agtgccgcgt ctttttcgat gtattggcgg
    18661 tattcgtcca aagtagtcgc accgatacag tgcagttcgc cccgcgccaa ggccggtttc
    18721 aacatattgc ccgcgtccat cgcgccgtcg gttttgcccg cgccgaccaa agtatggatt
    18781 tcatcgatga aaatcagggt gttgccgtcg tctttcgcca aatcgttcaa tacgcctttc
    18841 aagcgttctt caaattcgcc acggtatttc gcaccggcaa tcaaagccgc caaatccaaa
    18901 accagcaggc gtttgttacg cagggattca ggtacttcgc cgttgacgat gcgttgcgcc
    18961 aaaccttcga cgatggcggt tttacccaca ccgggttcgc caatcagcac ggggttgttt
    19021 ttggtgcggc gttgcagcac ctgaatcgcg cggcggattt catcatcacg accgataacg
    19081 gggtcgagct taccgtctcg ggcgcgttgg gtcagatcga gcgtgtattt tttcaaagca
    19141 tcgcgttggt cttcggcatt cgcatcgttc acgttttgtc cgcctcgtac cgcatcgatc
    19201 gcggcattga tgttttgttc ggtcgcaccg gcttctttca agattttgcc ggccgcatcg
    19261 ttctgctgca ccaaggcgag caggaaaagt tcgctggcga tataggcatc gccgcgtttg
    19321 gttgccgctt tgtccatcag gttcaacacc gcctgcaatt cgcggctggg cagaatctca
    19381 ccaccttgtc cggacacttt cggcaggcta ttcaaatgct gctgcaaacg ctgtttaacc
    19441 tgcggcacgt tcacgcccgc atgagccaag agcgcggcag ctccgctgtt ttggtcgtca
    19501 agcagggctt tcagtacgaa acctgcttcc agatagctgc tgtccgcagc caacgccaaa
    19561 ctctgagctt ctgcaagggc ttgttggaat ttggcggtta atttgtcgta tcgcattttc
    19621 gtttcctttt caaaatgtcc gctgtcgaag cctatatgtg catgactgtg gataactcaa
    19681 gtgttgtttt ctgtttttct atatttaatt cgatatattg ttgaatttaa agtatataga
    19741 aatgtatgat aatatgtata actatatctt cttaatatgg aaaagtctgt tgtcggctgg
    19801 atgtatgtgg caaatcgggt ataatcggcg catcttttcc ctttcagacg gcattgctgc
    19861 cgtaaggaca tttttatgag caaaaaacga gttctgaccg gcgtaaccac caccggcacc
    19921 ccgcatctgg gcaactacgt cggcgccatc cgccccgccg tccgcgcggc gcaaaacccc
    19981 gataccgaat ccttcctctt cctcgccgat taccacggca tcatcaaatg ccacgagcag
    20041 gagatgattc accaatccac ccaagccgtt gccgccactt ggcttgcctg cggactcgac
    20101 cccgagcgca ccaccttcta ccgccaaagc gacattcccg aagtgatgga gttgaactgg
    20161 attctgacct gcatcaccgc caagggtttg atgaaccgcg cccacgccta caaagccgcc
    20221 gttcaagcca atgcggaaaa cgggcaggaa gaccccgatt tcggtgtgga aatgggtttg
    20281 ttcagttatc cgattctgat gactgccgac attttgatgt tcaacgccaa cgaagtcccc
    20341 gtcgggcgcg accaaatcca acacgtcgaa atggcgcgcg acattgccgg ccgcttcaac
    20401 caccgtttcc aagaactctt caccctgccc gaagtgaaaa tcgacgaaaa cgtcgaactc
    20461 ttggtcggtt tggacggacg caaaatgtcc aaatcctacg gcaataccat tccgctttgg
    20521 gaaaacgaca aaaaaaccca aaaatcggtc aacaaaatca ttaccaacat gaaagagccg
    20581 ggcgagccga aacagcccga cgaaagccct ttgtttgaaa tctataaagc cttctccacg
    20641 ccgtctgaaa cggcggaatt tacgcaaatg cttgccgacg gcttggcttg gggcgaggcg
    20701 aaaaaacttt cggcggcgaa aatcaacgcc gaactggcag aactgcgcga acgctacaac
    20761 gcattgacct ctaatccttc gcaaatcgaa gagattttgc aggcaggcgc gcaaaaagcg
    20821 cgcaaagaag cgcgcgaatt gttggataaa gtacgcgatg cggtcggcat ccgcccgttg
    20881 aaataaaccc aatgccgtcc gaacctccgc ctgccacgtt tcagacggca tttcagacgg
    20941 cattttgaaa ccatcaggag tgcggaatgc tgaaaatacc ttttgccgtg ttgggcggct
    21001 gcctgctgct tgccgcctgc ggcaaatccg aaaatacggc ggaacagccg caaaatgcgg
    21061 cacaaagcgc gccgaaaccg gttttcaaag tcaaatacat cgacaatacg gcgattgccg
    21121 gtttggcttt gggacaaagt agcgaaggca aaaccaacga cggcaaaaaa caaatcagtt
    21181 atccgattaa aggcttgccg gaacaaaacg ccgtccggct gaccggaaag catcccaacg
    21241 acttggaagc cgtcgtcggc aaatgtatgg aaaccgacgg aaaggacgcg ccttcgggct
    21301 gggcggaaaa cggcgtgtgc cataccttgt ttgccaaact ggtgggcaat atcgccgaag
    21361 acggcggcaa actgacggat tacctgattt cgcattccgc cctgcaaccc tatcaggcag
    21421 gcaaaagcgg ctatgccgcc gtgcagaacg gacgctatgt gctggaaatc gacagcgagg
    21481 gggcgtttta tttccgccgc cgccattatt gagttattcg gacatcacgg aatatatttg
    21541 gatttttcaa accttgcgga aaaagtccgc gccgccggga aactcaaaag gaggggatgt
    21601 gtgttacaat tttccgaact gttttcataa aatagttttc ggacgtgttt caatatggca
    21661 ttgatgccgc cttgttgttc gagaaaaaac cctttatatt taatataatg ggttttaact
    21721 aaacgggaaa ccgttttctc tccggccgat gggcaaaatc agccgattga tggaacacgg
    21781 tccggttttt aagcaaaacc ttaatcaaca actcaagaag gataagacat gaaaaaatta
    21841 ttgattgccg caatgatggc ggctgccttg gcagcttgtt cgcaagaagc caaacaggag
    21901 gttaaagaag cggcccaagc cgttgagtcc gatgttaaag acactgcggc ttctgccgcc
    21961 gagtctgccg cttctgccgt cgaagaagcg aaaggccaag tcaaagatgc tgcggctgat
    22021 gcaaaggcaa gtgccgagga agctgtaact gaagccaaag acgcggcagc cgaaaccaaa
    22081 gaagcggtaa gcgaagcggc taaagacact ttgaacaaag ctgccgacgc ggctcaggaa
    22141 gcggcagaca aaatgaaaga cgccgccaaa taatttgttg ccttggcaaa aatgacgaga
    22201 tgccgcctgc cggcaaccca aatgaaaccg cctgaagatt ttcaggcggt ttttgagttg
    22261 tatgcccctt gttttttacg cctgcatgac cgtgcaggga ggatgtgccg cgcatatcgg
    22321 gatattgcgg acagggaaat gccgtctgaa catcatttag acggcatttg gtttgtcaag
    22381 gttcagacgg cttcaacttt gaacggcata ttgatttttt gccattggac gctttcatat
    22441 tccaatgcgt cggcaatcag ggggtggcgt tccagccatt tcctgtcaat acgcaggatg
    22501 aagccgcagc tttccgtatc cgtacgcaac tgcatatttt tcgggaaacc cagatcttgg
    22561 cgcgaacggc agaacagggc ggcaaggcgc agggacaaaa cggcatacca caacatttcg
    22621 ttgccgctga taatgccgcc catttttttc atatcgccgc gatgaccgat gaccagttgg
    22681 gcaagtatgg tctgttcctt gcgtgaaaaa cccggcatat cggcgttttc gaggatgtag
    22741 gcggaatgct tgtgatagcc ggtgtgggcg atgtccaaac cgatttcatg cagcgcggcg
    22801 gcgcgtccga gatactgttg ccacaaggca agttcttgaa ccgtaacgtt tttggcatga
    22861 cagaggctgt ccataaaggt ttgcgcggtc tcggcggtgc gtttcgcctg attgaggctg
    22921 acgtggtagc ggtgttggaa ctcggcaacc gtttgtccgc gcatatcttc gtttaagccg
    22981 cgcccgatca aatcgtaaaa cacgccgtcg cgcagggcgg cttcggttac ggtcatcctg
    23041 tcgagtttca tttcctcaaa cgccgccatc atcacggcaa gtccgccggc aaaaacttcg
    23101 atgcgttccg gtttcaggtt ttcaaatttg gcttttttga ccgaaccggc ttcgatgatg
    23161 cgttcggcga gggcgcgcat tcccttgtag gtaatgtccg cctcttgggg catttcggcg
    23221 gcaagcacgt cgcggatgga ttttgccgaa cccgatgtgc cgacggcgaa atcccaacct
    23281 tcgcgcctca tatttttgct gatgcgctgg atttcgttgc gggcggcgga aatggcggct
    23341 tggaagtctt tggcggtgat tttgttttgg aagaagcgca ggctgtaggt tacgcagccc
    23401 aagggcaggc tttcggtaat gtcgggattc agcgtcgaac cgatgacaaa ttctgtcgaa
    23461 ctgccgccga tgtcgataac cagcattttg cccccgcagg gggggagggt gtggatcacg
    23521 ccggtataaa tcagccgcgc ctcttcgcgc ccggcgatga tttcgatggg gaaacccagt
    23581 gccgcttcgg ccttgggaag gaaatctgcg atgtttttgg cgacgcggaa tgtattggtt
    23641 gccacggcgc gcacctgctc ggggcggaag ccgcgcaggc gttcgccgaa ttttgccagg
    23701 cagtccaaag cctgttcctg cgaggatgtg ctgaggtttt tctgttcgtc cagaccggcg
    23761 gcaaaacgga ccatctgttt gaaggaatcg atgactttta attgtccgtt gttgttttcg
    23821 caaatctgga ggcggaaact gttggaaccc aaatcgacgg aggcgaggac gtttgcggga
    23881 ttggtggtca tggcggatac cggtggggga aaaacgcaat gttactctga cggcgcaggc
    23941 gttgacaata aatgatgcgg ctgtttttga ttctgctcac ggatgttgcc gacggtatgt
    24001 ttccgacagc aaaaaatgcc gtctgaaaaa cgttcggacg gcattttttg cgcttatttg
    24061 aaatcctttt ccacgctcat gaaaatctgc gtatttttgc gtgtgtaaaa acttttcata
    24121 ttgctgtcga ttttcagata gcggaaattg agttgcggcg taaagccctt ccaagagatt
    24181 ttgtcgtgcc gcaacgacag gtttgcctga tattcgtggt ctttgcgcgg gaagcggtac
    24241 acaattgtcc cgggtgcatc aaaagtcctg cgggtatagc gcaggtttgc ccgcagcccc
    24301 aagccgccgt cgaacgtttt gaccgcgccg acacgcaaac ccttgcggac ggaagcctgt
    24361 tccgcctctt tcgtcatgtc gtgcggccag tccgcaccgc cgtaaagcag ccggtctttc
    24421 ggcgcggaat acatcagcgt cgcgcccgcc agcggcatat ggctgccgta tcgggcggcg
    24481 gtgcggtctt cctgataatg cttccacata ttgcccgcgt tcagcgtcag ccgccagcgt
    24541 tcgcccaagc gtcgggagaa atcggcattg aagccgccga cgaaattgta tcggctgccg
    24601 cctaagaggt tttgcccgac aaacggcacg atgccgaacg agcgcgttac cgaacgattt
    24661 ttatagccga gcgacaggtg caggcactgt tcgctgaaat ctttgttatc ccaataatgc
    24721 acgccgccgc cgctgatgcc gccgtagagg aaatgatgcc cgcccgcatt gatttcgcgc
    24781 gacacgccta agccctagcg caaaccgtgt gccttttgcg gcaggctgtc ggcggttttc
    24841 gtccagcttc tgcccgcaaa ttcaatcgtt ttttcggacg aagcgtt
//
LOCUS       DAWTCI010000022        24611 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWTCI010000022 DAWTCI010000000
VERSION     DAWTCI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24611
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.022"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            33..1340
                     /gene="mafB"
                     /locus_tag="WM481_000898"
                     /pseudo
     CDS             33..1340
                     /gene="mafB"
                     /locus_tag="WM481_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            1382..1918
                     /locus_tag="WM481_000899"
     CDS             1382..1918
                     /locus_tag="WM481_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HFA9080245.1"
                     /translation="MFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQ
                     REWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIK
                     KPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTSKSG
                     IKIEGFTEPNRTAYPIYE"
     gene            1949..2368
                     /locus_tag="WM481_000900"
     CDS             1949..2368
                     /locus_tag="WM481_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080246.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            2446..2529
                     /locus_tag="WM481_000901"
                     /pseudo
     CDS             2446..2529
                     /locus_tag="WM481_000901"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2526..2768
                     /locus_tag="WM481_000902"
     CDS             2526..2768
                     /locus_tag="WM481_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HFA9080247.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2846..3091
                     /locus_tag="WM481_000903"
     CDS             2846..3091
                     /locus_tag="WM481_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9080248.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGKDKI
                     PSYTWHHHQDTGRMQLVPEWEHSKTGHIGGTAMGKGK"
     gene            3094..3480
                     /locus_tag="WM481_000904"
     CDS             3094..3480
                     /locus_tag="WM481_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080249.1"
                     /translation="MWKIYKENSTDLNFAIGSIYCQAINLTEFKMWVEKIIREVDLDE
                     IPNYFFDLIDFQSLYDLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPISKEKA
                     LKALEKHPEIYQKFQHFFPFVELPPL"
     gene            3555..3698
                     /locus_tag="WM481_000905"
     CDS             3555..3698
                     /locus_tag="WM481_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9080250.1"
                     /translation="MFDGKGTSEIPNYTWHHHQDTGRMQLIREDSHHDTDHIGWRAMS
                     KGK"
     gene            3701..4091
                     /locus_tag="WM481_000906"
                     /pseudo
     CDS             3701..4091
                     /locus_tag="WM481_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4131..5588)
                     /locus_tag="WM481_000907"
     CDS             complement(4131..5588)
                     /locus_tag="WM481_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFA9080251.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHALATL
                     SLGGFSTHDASIGYYNSPLIEAVIIVFTIFGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGHYAGFGESLRYTAFNFVSIGLANGLSNTDFAQWPLLIS
                     LWMFFLANILASSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTSAYWKK"
     gene            complement(5603..6433)
                     /locus_tag="WM481_000908"
     CDS             complement(5603..6433)
                     /locus_tag="WM481_000908"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFA9080252.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLYLLAVGCGEGALKRSDTIEPILKHPDGGKMLDWL
                     RAQPLLIREGGRVMIHAGILPQWRIAKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(6510..6899)
                     /locus_tag="WM481_000909"
     CDS             complement(6510..6899)
                     /locus_tag="WM481_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9080253.1"
                     /translation="MSKTVIHTDKAPAAIGAYSQAVRAGGTVYMSGQIPLDPATMTVV
                     GNGDFHTEARQVFQNLQAVAEAAGGSLDDIVKLNAYLTDLGNFAVFNEVMAEFITEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(7046..7573)
                     /gene="nspA"
                     /locus_tag="WM481_000910"
     CDS             complement(7046..7573)
                     /gene="nspA"
                     /locus_tag="WM481_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFA9080254.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKQAPSTDFKLYSIGASVIYDFDTQSPVKPYF
                     GARLSLNRASAHLGGSDSFSKTSAGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNNVK
                     NVRSGELSAGVRVKF"
     gene            complement(7957..9132)
                     /locus_tag="WM481_000911"
     CDS             complement(7957..9132)
                     /locus_tag="WM481_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HFA9080255.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYSLPNQTVQTALDDVQTAIATDATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGTLEGAGFIHYETSAFAKPTMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPGEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPAAKIMVQIETARQKGLLETDPTVFRPTEKGRLFLNDLLQCFL"
     gene            complement(9189..11660)
                     /gene="ligA"
                     /locus_tag="WM481_000912"
     CDS             complement(9189..11660)
                     /gene="ligA"
                     /locus_tag="WM481_000912"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFA9080256.1"
                     /translation="MNPTAQRIHELTDLLNRYAYEYYTLDAPSIPDAEYDRLFRELEA
                     LERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPMLSLTNAFSPQDENGVFDHAEMYA
                     FDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVLVQAATRGDGTTGEDVTRNVKTV
                     SNIPLRLHGENVPELIEVRGEVLMLKADFAGLNKRQAENGQKPFANPRNAAAGSLRQL
                     DSRITAQRKLHFFPYSIARQQGGFEAEEHIQELAYFQELGFSLPNGNFGCFKNIGEVL
                     AFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFISRAPRWAVAHKFPAEEALTIVE
                     AIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQDEVSRKDVRVGDTVVVRRAGDV
                     IPEVVRVIFERRPMQETAVAVSDGIGHQQDDLFAETPSAKQTESVPLHKPYRLPARCP
                     ICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFASRKAMDIDGLGEKQIEQLVAQDL
                     VRHFADLYRIDIPTLQKMKETADKGSSENENGDAETVSGDLSKYNTQNGKKQPTKWAQ
                     NILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGTLERVRRAPEPVLACLPDI
                     GTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPAAQYAGPQRWITRLPGFKI
                     SENKAQALWELAGQSIEGLQNDKALPADWQAWRSKAQNTALLENLKTFFAQMPSEDEA
                     AQGSDGINKAVAGKTFVLTGTLPTFKRDQAQALIEAAGGKVSGSVSKKTDYVVAGEAA
                     GSKLEKANALGVSVLSEAELLTLLC"
     gene            complement(11794..13080)
                     /locus_tag="WM481_000913"
     CDS             complement(11794..13080)
                     /locus_tag="WM481_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFA9080257.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKPAKPQDSAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEEIGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQADAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(13274..13846)
                     /gene="ampD"
                     /locus_tag="WM481_000914"
     CDS             complement(13274..13846)
                     /gene="ampD"
                     /locus_tag="WM481_000914"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFA9080258.1"
                     /translation="MDNHAGAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLHVSSHFLIARDGETVQFVSCGDMAYH
                     AGASSFRGREKCNAFSIGIELEGCDFEPFAEAQYRSLETLLAALCRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            13930..14925
                     /gene="mltG"
                     /locus_tag="WM481_000915"
     CDS             13930..14925
                     /gene="mltG"
                     /locus_tag="WM481_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFA9080259.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIGHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWAGRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            14985..15605
                     /locus_tag="WM481_000916"
     CDS             14985..15605
                     /locus_tag="WM481_000916"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFA9080260.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEEVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQAREKDRFEQEQAD
                     FFMRVRGVYLDRAAACPERYAVIDSNRSLDEVRNSIEKVLDGHFGC"
     gene            15827..17107
                     /locus_tag="WM481_000917"
     CDS             15827..17107
                     /locus_tag="WM481_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HFA9080261.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRYTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGRRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLGEWKL"
     gene            17514..18545
                     /locus_tag="WM481_000918"
     CDS             17514..18545
                     /locus_tag="WM481_000918"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFA9080262.1"
                     /translation="MPNFFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKWRADF
                     LSGKRQSEKLSVPVVVVGNIHAGGTGKTPIAAALVSGLQEKGVKVGIISRGYGRKSKA
                     VYVLNAASRAEDAGDEPLLLFRKTGAPTAVGSSRVEAGRALLAAHPELELIVADDGLQ
                     HYALQRDVEIAVFPAADTGRTDLDLLPNGNLREPLSRLESVDAVVVGGRAADGFMPSE
                     HLFGSRIEAGAVYRLNRPSEKLDISTLSGKRVAAVAGIARPQRFFDTLTHMGIRLDQT
                     VALPDHADIFNRDLPPADVVLVTEKDAVKFSDGICTDNVWVLPVCAIIEPDLAEFVLE
                     RLEGVPKAV"
     gene            18751..19284
                     /locus_tag="WM481_000919"
     CDS             18751..19284
                     /locus_tag="WM481_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFA9080263.1"
                     /translation="MKLKTLLLPFAALALCANAFAAPPGDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGIVD"
     gene            19269..19376
                     /locus_tag="WM481_000920"
                     /pseudo
     CDS             19269..19376
                     /locus_tag="WM481_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            19506..19688
                     /locus_tag="WM481_000921"
     CDS             19506..19688
                     /locus_tag="WM481_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFA9080264.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            19685..20446
                     /gene="kdsB"
                     /locus_tag="WM481_000922"
     CDS             19685..20446
                     /gene="kdsB"
                     /locus_tag="WM481_000922"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9080265.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTACQAHGIEVVMTSNRHESGTTRLAEAAAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAGHELHDFDELMNPNAVKVVLDKNGNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRVGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            20469..20765
                     /locus_tag="WM481_000923"
     CDS             20469..20765
                     /locus_tag="WM481_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFA9080266.1"
                     /translation="MQQHIEKWQHLSREEQKILAEVWGLVQNDDQEVHYEMLKLNAPD
                     EVSGEFWFRMAETLSTLPPNRSLDLRMNGGRLSTAVSILSVMIEDNPDIPQLWA"
     gene            20787..20984
                     /locus_tag="WM481_000924"
     CDS             20787..20984
                     /locus_tag="WM481_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080267.1"
                     /translation="MVHGHKARADGLVQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            21141..21233
                     /locus_tag="WM481_000925"
     tRNA            21141..21233
                     /locus_tag="WM481_000925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21175..21177,aa:Ser,seq:gct)
     gene            21507..22292
                     /locus_tag="WM481_000926"
     CDS             21507..22292
                     /locus_tag="WM481_000926"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFA9080268.1"
                     /translation="MSRIRQAFAALDGGKALIPYIAVGDPDIRTTLALMHGMVASGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PIHKMGYREFAQEAAKAGVDGVLTVDSPIETIDSLYRELKDNEVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLRQYIDIPIGVGFGISNAESA
                     RKIGRVAAAIIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            22329..23201
                     /locus_tag="WM481_000927"
     CDS             22329..23201
                     /locus_tag="WM481_000927"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HFA9080269.1"
                     /translation="MSWLDKILPPKIKNRGKDGSSNVPEGLWRKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPTDPLKFKDSKKYPDRLSAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAT"
     gene            complement(23261..23872)
                     /locus_tag="WM481_000928"
     CDS             complement(23261..23872)
                     /locus_tag="WM481_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFA9080270.1"
                     /translation="MRRAILLILTLTVGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAELPPLPDAHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCALSSIGFGNGKLSSYKVFGYGDTVNSRWIQPRNAEWKPIGGTLGRN
                     DALRAVLYQAFCEGGVPADTQGLVQRLKERAGRYAPSMKPHDK"
     gene            23980..24204
                     /locus_tag="WM481_000929"
     CDS             23980..24204
                     /locus_tag="WM481_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFA9080271.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMRQGEVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            24235..>24611
                     /locus_tag="WM481_000930"
     CDS             24235..>24611
                     /locus_tag="WM481_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080272.1"
                     /translation="MLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLFRSACVLTQGS
                     FEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYLSLTRDSCSVK
                     AERADSAALLEHFSGLVRQPPKNA"
BASE COUNT         5718 a         5848 c         7222 g         5823 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 atccctttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggag cttgccgagc gcagcggcca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgacgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc tttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtca tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaatc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccgtca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgcg atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gaaagaatgt caaactggca gaccaacgcc
     1261 acccgaagac aggcgtaccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggc ggtataccta aggctaagcc
     1381 tgtgtttgat gcgaaaccga gatgggaggt tgataggaag cttaataaat tgacaactcg
     1441 tgagcaggtg gagaaaaatg ttcaggaaac gagaagaagg agtcagagta gtcagtttaa
     1501 agcccatgcg caacgagaat gggaaaataa aacagggtta gattttaatc attttatagg
     1561 tggtgatatc aataagaaag gcacagtaac aggagggcat agtctaaccc gtggtgatgt
     1621 acgggtgata caacaaacct cggcacctga taaacatggg gtttatcaag cgacagtgga
     1681 aattaaaaag cctgatggaa gttgggaggt gaaaacgaaa aaaggtggga aagtgatgac
     1741 caagcacacc atgttcccaa aagattggga tgaggctaga attagggctg aagttacttc
     1801 ggcttgggaa agtagaataa tgcttaagga taataaatgg cagggtacaa gtaaatcggg
     1861 tattaaaata gaaggattta ccgaacctaa tagaacagca tatcccattt atgaatagta
     1921 atatttatga aaaattagga gattaatgat gaaaagaatt aagtgctttt gtgataaatt
     1981 tccatcagga gatacattta gaatgtgtat cattctggat gactatgata atagggttga
     2041 ttattatgta ggaatatatg attacattac gtctacctta atgagcgata tttactatcg
     2101 atccacgatt gatgagcatt tcaagattat agaattaata gaaaataatc caaatgaaat
     2161 ttatgatgat ggcggtggtc aacaattttg cctagaattt catcatgata aggtcatttt
     2221 ttatcacaat gaatttgatg aagaagatgg ttatccagta ttaagctgtt cgctgcatac
     2281 ttttaaaact gctttaattg cttggaatgc ttttttgcaa ttgcctaaaa gtattcattc
     2341 ggtggtggag actgtgattg aggaataagc ataattagct taatgaatag aatcagcgat
     2401 atagattgga ctgcaaatcc acgcttatac gctgtgccat gattaacagg aagagagcaa
     2461 cttggatggg ctagtaggga tggtaagcat ttaaatatat caattgatgg aaagattaca
     2521 cacaaatgac taatttaaaa ttagatttct actctgaagt tattataaaa gattcttgcc
     2581 ctaatgattt gttagaaaat ggtgagacta ttaaaggaaa aaaaggagtg gtattaggta
     2641 taagcgaaga agatggtata atctatggct atactatttt actttttgat ataaaatatt
     2701 gtatatatat agataagaaa tatattatac cgactggaaa aaaattctct cgcgatgatt
     2761 tttattagct aatttaagta atttaagcaa gttttttaga tactgaaact ggtaattatg
     2821 tttatgtaag cataaggagt tcagattgaa ggattctaca gggcaaatga ggctggcaac
     2881 caaggatttg gcggaagcca ttaaacgagg agaagtacgt agttctgctt ttacaacaaa
     2941 gcaactaaag gcaatcgaaa aaggcaaaga caaaatccct agctacactt ggcatcatca
     3001 tcaagataca gggagaatgc agcttgtgcc tgaatgggaa cattctaaaa ccggtcacat
     3061 aggagggacg gcaatgggga agggtaaata attatgtgga aaatttataa agaaaatagc
     3121 accgacttaa attttgccat aggcagtata tattgccaag caatcaatct taccgaattt
     3181 aaaatgtggg tcgaaaaaat cataagggaa gtggatttag atgaaattcc aaattatttt
     3241 tttgatttga tagattttca atcactatac gatctgattg atattatagg atttgttccc
     3301 gaaaataact tatcaaaaaa tcaggataat gcattaaccg gtattgcttt tttaaggggg
     3361 atagatgtct acgatcctcc catttcaaaa gaaaaagcat taaaagcctt agaaaaacat
     3421 cctgaaattt atcagaagtt tcagcatttc tttccgtttg tagagctgcc tccgctttaa
     3481 acagtcaaaa tgccgtctga aacgatattc ggctttcaga cggcattttt gatataaagc
     3541 ggataaccaa agaaatgttt gacggcaaag gaacatctga aataccaaat tacacttggc
     3601 atcatcatca agatacagga aggatgcaat tgattcgtga agactcgcat catgataccg
     3661 accatatcgg ttggagagcg atgagtaaag gaaagtaact atgtggaaaa tcataaaaga
     3721 ggatagtgat gatttaggat ttgcaattaa atgcttattc tctcaatcta ttgatttaaa
     3781 tgaattcaag ttatggattg aacaagtaat acgcgatatg cccatcgagg acatcccttt
     3841 ttatattttt gacttggcgg atttcaatgg gggaattgga gacattgaca atattgtagg
     3901 ttttgtttcg agttacagcc tatcaaaatc gaaaaaaaat gccttgacag gcattgcctt
     3961 cttaaggggg atagatgtct atagaccctc ccgtttcaaa agaaaaagca ttaaaagcct
     4021 tagagaaata tcctgaaatt tatcataaat ttaaacggtt ctttcctttt gtagagcttc
     4081 cgcttcttta aaggacaata tgccgtctga aaagttttca gacggcattt ttatttcttc
     4141 cagtaggcgg aggtgaagag gatgaagacg gtgaagattt ccagcctgcc caagagcatt
     4201 gcggtaacgc agatccattt ctgcatcacg tccaaaccgg cgtaattgcc ggcgggcccg
     4261 acttcgccca gtccggggcc ggcgttggta atgcaggcga tgacggcggt aaaggcggtg
     4321 gtaaattcca taccgctcgc catcagcagg aagctgaaga ggacgacggt cataaagtag
     4381 atgaagatga aggacataac ggtcagcgcg aggcggtcgg gtatggcttt gccgctgatt
     4441 ttgacggtgc ggacggcttt ggggtgcagc agcaccatca tttcgcgcag gctgaatttg
     4501 aacaggacga gggcgcgtat ggttttgatg ccgccgccgg tcgagccgga gctggcgagg
     4561 atgttggcga ggaaaaacat ccacagggaa atcaggagcg gccattgtgc gaagtcggtg
     4621 ttggacagcc cgtttgccaa tccgatggag acgaagttga aggcggtgta gcgcagggat
     4681 tcgccgaagc cggcgtaatg gccggtgtgc cacaggtaca gggcggcggc aaggatgctg
     4741 ccggagagca gcagcagcat cgtccggcat tcttcgtctt tccaataggt tttgaggctg
     4801 cggctgttga gggcggcgaa atggctggca aaattgatgc cgccgaaaat agtgaaaacg
     4861 atgatgactg cttcgatgag gggggagttg taataaccga tgctggcatc gtgggtggaa
     4921 aacccgccca gcgagagggt tgccagcgcg tgacagacgg catcgaacca acccatcccg
     4981 gcaaaatgca ggcaggctgc cgcgaggatg gtgatcaggg tgtagccgaa ccagagtttt
     5041 ttcgccactt gggaaatgcg cggcgacatt ttgctttctt tgtcaatgcc ggggatttcg
     5101 gctttgaata actgcgtgcc gcctacgccg agcataggca ggatggcgac ggcaaggacg
     5161 atgatgccca tcccgcccag ccagttgagc atatgccgcc aaaagttgac ggagggggcg
     5221 agcccgtcga cgtgggggat gacggtcgcg ccggtggtgg tcagtcccga catcgattca
     5281 aaaaatgcgt cggtaaagcc catattcggg aaatacaggt acatcggcat cgcagccata
     5341 gcggcaaacg ccagccacaa catcaggacg agggtgaagc cgtcgcgcgg gcgcagttcg
     5401 cgcctgaacc ggagggtggc gagccggacg atgcacgagc cggaaagggt aacggtcgcg
     5461 gtggtggcga atgcggtgta cgcgccgtcc gaaaaggcgt aggaaagggc ggcgggtatc
     5521 agcaggataa aggaaaacag catacccagt cgggagagga cgtgggcgat gggcaggatt
     5581 ttgtgcatag tggggcggtc cgttattttg cgaagctttt ccagtctatg ccgccggcgg
     5641 cttggacttg ggtaatttct tccgtttcga ggttgacggc ggtcagctgc ccgccccaca
     5701 gcgcgccggt gtccagcgag atgacgttgt cggcattcgt gtagcccagc gaggaccagt
     5761 gtccgaagat gatggtgtgg tcgaggtttt gccgatcggg ggctttgaac cacgggcgca
     5821 ggtaaggcgg catttttttc actgtggatt tgtagtcgaa atccagttcg tttttaaagg
     5881 tcagggcgcg catccgcgtg aaggcgttga cgatgaagcg caggcgggca tagccttcca
     5941 aaccttcgtc ccatgcggcc ggtttgttgc cgtacatttt ggagaagaat ttgacgtatt
     6001 ttttgccgcg cagttcggct tcggcttctc cggcgagcga ttcggctttg gctatgcgcc
     6061 attgcggcag gatgccggcg tgtatcatca cgcggccgcc ctcgcgtatc aaaagcggtt
     6121 gcgcgcgcag ccagtcgagc atttttccgc cgtcggggtg tttgagtatg ggttcgattg
     6181 tgtcgctgcg tttgagcgcg ccttcgccgc agccgacggc gagcaggtac aggtcgtggt
     6241 tgccgaggac gatttgcacg ctgttttcgt gccggatgca gaattgcagc gtttcgaggg
     6301 atttcgggcc gcggttgacg atgtcgcccg tcagccagag ggtgtccgtg ccgtggttga
     6361 agccgatttt gccgagcagc gcggtcagtt cgtcgaaaca gccttggatg tcgccgattg
     6421 cgtaatgtgc cattgcgggt gttgtgaagt gggaaagtgt tgcggttcgg acggcacggt
     6481 tggaaatctt atgccgtccg aacgtggaat tatgcgttca aaacgaggac ggcttccgct
     6541 tcgacctgca cgcctttggg cagcgaggca acgccgacgg cggcgcgggc ggggaacggc
     6601 tcggtgataa attccgccat cacttcgttg aagacggcaa aattgcccaa gtcggtcagg
     6661 taggcgttga gtttgacgat gtcgtccagc gaaccgcctg ccgcttcggc gacggcctgc
     6721 aggttttgga acacttggcg cgcttcggtg tggaaatcgc cgttgccgac cacagtcatc
     6781 gtggcgggat cgagcggaat ctgaccgctc atgtaaacgg tgccgccggc gcgcacggcc
     6841 tggctgtatg cgccgatggc ggcgggggct ttgtcggtat ggatgacggt tttggacatt
     6901 tcggattcct caaaaaatag ggcggcagaa gccgcagcgt tcgggattat cgtacaaaac
     6961 cgccggcttg tgtagtttta ggcggtatat gtgccggaaa caaaaaaccg ccgaaggctc
     7021 ggcggtttgc ggaataacgc gtatatcaga atttgacgcg cacgccggcg gacagttcgc
     7081 cggaacggac gtttttgaca ttgttgactt tgccgatgta gttgtagcgg tagccggcat
     7141 ccagatcgac attgggtgta acggcatagc ttacgcccgc caatacgccg aggccggcgg
     7201 aggttttgct gaagctgtcg ctgccgccca agtgggcgga agcgcggttg aggctcaagc
     7261 gcgcgccgaa atacggtttg acgggcgatt gggtgtcgaa gtcgtaaatg acggacgcgc
     7321 cgatgctgta aagtttgaaa tcggtggatg gggcttgttt atagtttttg tagcgcgtgt
     7381 aatcgacggc gaagcggagg tcgttgatgc ggtagcctgc ggagatgcgc gggctgaagc
     7441 ctttggcaga acctaaagag cttgaggctt tggcgtgtgc ggcatcggct tggacgtaaa
     7501 agccggatgc gccttccgcc agtgcggcgg ccgggagtgc gagggcaatc agtgcggcaa
     7561 gtgctttttt catattttgg ttcctttatg gtcagggaga aaaattgtta ataatccgtt
     7621 aaagaatcct gccgtattat actgaaattt tctttttgca tcgtaatatt ttcaatgcct
     7681 caagatacgc ggcggcatca cgtggcggat gggtgaaact tgcaaacggt ttggaaaaaa
     7741 ccgcaaaggc ggggcggcgg catttgtcag attgttgcag gcgcaggcgt acggtttttt
     7801 gtgtgcgggg ttaccttagg cgtcggacat ttccggcggc ggctgtgccg tctgaaacgc
     7861 ccggcggggg atgcggctgc gtttcccatc gataggcata tttgccggcc gcgttcgggg
     7921 cgggttttac ccgggcggcc gccgatttgt ttgcgcttat aaaaaacact gcaacaaatc
     7981 gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg gtcgggtcgg tttccagcag
     8041 gcctttttgc cttgccgttt cgatttgcac catgattttg gcggcgggta cgcccgtgcg
     8101 ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc agggcgttca tcatgaattc
     8161 gaacggcaaa tcttcggcgg cgacggtttt gcgttcgacg gcttcgcccg gctgactttg
     8221 cattaaggcg aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga tgcggtcggg
     8281 ataggagatt ttgccgtgcg cgcccgcgcc tatgcccaaa taatcgccga actgccagta
     8341 gttcaaattg tggcggcact gcatggtggg tttcgcaaaa gccgatgttt cgtagtggat
     8401 aaaacccgcg ccttccagtg tgccgtgtac cgcgtcttcg atgtcgagcg ccgcttcgtc
     8461 ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg ttcggctcca tcgtcagatg
     8521 atacgcgctg atgtgggttg cgtctgtggc gatggcggtt tgcacatcgt ccaatgccgt
     8581 ctgaacggtt tggttcggca gggagtacat caagtcgata ttgactttat caaataattt
     8641 taaggcggta tcgatagcgg ttaaggcttc tttgccattg tggacgcgcc ccagccttga
     8701 gagcatatcg tcgttgaaac tctgtacgcc gatagaaagc cgcgtgatgc ctgcgtcttt
     8761 aaatccttga aacttctcga tttcaaatgt acccggattg gcttccaacg taatttccgc
     8821 ttcgggctgc aagcgcaaca gcgaacgcac gccgcttaac aaacggtcaa tcgattccgc
     8881 ctgaaacagg ctgggcgtac cgccgccgaa aaatatcgtt tccaccggcc tgccccaaat
     8941 attgggcaat tcaagctgca agtcggtcag cagcgcgtcg atataggcgg cttcgggcaa
     9001 tccgtttttc aggctgtggg aattgaagtc gcaatacggg cattttttga tgcaccacgg
     9061 gatgtggata tagagcgaca ggggcggcag ggcggtgagt cgggtgcggt ttggaaagga
     9121 aatggtgtgc atggtgcggt tcggaaaagt ggacgatgcc gtctgaaggc ggttcggacg
     9181 gcatgggttc agcagagcag ggtaagcagt tcggcttcgc tgaggacgga aacgcccaag
     9241 gcattggctt tttccagctt gctgcccgcg gcttctccgg cgacgacgta gtcggttttt
     9301 ttggacacgc tgccggacac tttgccgcct gcggcttcga ttagggcttg ggcttggtcg
     9361 cgtttgaagg tgggcagggt gccggttaac acaaaggttt tgcctgccac ggctttattg
     9421 atgccgtctg aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa gaaggttttt
     9481 aggttttcga gcagggcggt gttttgcgct ttgctgcgcc acgcctgcca gtcggcaggg
     9541 agggctttgt cgttttgcag tccttctata ctttgtccgg cgagttccca taaggcttgg
     9601 gctttgtttt cgctgatttt gaaaccgggc aggcgggtaa tccagcgttg cggcccggca
     9661 tattgtgcgg caggcaggct gacggcttgg gcttgcgggg caacgcctgc ggcgagcagc
     9721 tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga acgcgccact
     9781 accgtgccga tgtcgggcag gcaggcgagg acgggttcgg gggcgcggcg gacgcgttcc
     9841 aatgtgccga atgcctgcgc cagcgttttg gcggtgcgtt cgccgacgtg gcggatgccg
     9901 agcgcgaaca ggaagcgggc aagttcgggc gttttgccgg attctatgcc tgcgaggatg
     9961 ttttgcgccc acttggtcgg ttgttttttg ccgttttggg tattgtattt agacaggtcg
    10021 cctgaaaccg tttctgcgtc gccgttttcg ttttcagatg accctttatc cgccgtttcc
    10081 ttcatttttt gcagggtcgg aatatcgatg cggtagaggt cggcgaaatg gcggacgagg
    10141 tcttgggcga ccagctgttc gatttgtttt tcacccaagc cgtcgatgtc catcgctttg
    10201 cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat accgccgctg
    10261 catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat ggggcagcgg
    10321 gccggcaggc ggtagggctt atggagcgga acggattcgg tttgcttggc ggacggtgtt
    10381 tcggcaaaca ggtcgtcctg ctgatgcccg atgccgtctg aaacggcaac ggcggtttcc
    10441 tgcatcgggc ggcgttcgaa aatcacgcgc acgacttcgg gaatcacgtc tccggcgcgg
    10501 cgcacgacga cggtatcgcc gacgcgcacg tctttgcgcg atacttcgtc ctgattgtgc
    10561 agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgcaggcg ggcaaccggc
    10621 gttaccgcgc ccgtcctgcc gatttgcacg tcaatcgctt cgacaatggt cagggcttct
    10681 tcggcaggga atttgtgggc aaccgcccaa cgcggcgcgc gggagatgaa gccgagttcg
    10741 cgctgttgcg ccaagctgtt gactttgacc accatgccgt cgatttcgta gggcagttcg
    10801 gggcgttttt gctgcatgtg ttcgtaaaac gccaatactt cgccgatatt tttgaaacag
    10861 ccgaaattgc cattgggcag actgaagccg agttcttgga aataggcgag ttcttggatg
    10921 tgttcttcag cttcgaaacc gccttgctga cgggcaatcg aataggggaa aaagtgcagt
    10981 ttgcgttgcg cggtgatgcg cgaatcgagt tggcgcaggc tgccggcggc ggcgttgcgc
    11041 ggattggcaa agggtttttg cccgttttcg gcttggcgtt tattgagccc ggcaaagtcg
    11101 gctttgagca tcagcacttc gccgcgtacc tcgatgagtt cgggcacgtt ttcgccgtgc
    11161 agccgcaggg ggatgttgga tacggttttg acgtttcggg taacgtcttc gcccgtcgtg
    11221 ccgtcgccgc gcgttgccgc ctgcaccaat acgccgtcgc ggtagagcag gctgatggcg
    11281 aggccgtcga atttgggttc gataacgtat tcgggattgc cgccgtccaa gccgtcgcgc
    11341 acgcgttggt cgaaggcgta catttcggca tggtcgaaca cgccgttttc atcttgcggg
    11401 gaaaaggcgt tggtcagcga cagcatcggc acttcgtggc gcacttcggc aaagcctgcc
    11461 aaaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gcttgagctc gggatggttt
    11521 cgttccaacg cttcgagttc gcggaacagg cggtcgtatt cggcatcggg tatgctgggc
    11581 gcgtcgaggg tgtagtattc gtaggcgtag cggttgagga ggtcggtgag ttcgtgtatg
    11641 cgttgtgcgg tcgggttcat ggattcgtac aggttcagac ggcatggaca aatgccgtct
    11701 gaacggtaaa acgtgttgga aatggtcgga ttttacctga aaacaaaagg cggatgcacc
    11761 gttgccgatg tatccgcctt tgtgtttgcg gagttatgaa aacaggcgca gggcggtttt
    11821 gccgcccggt tcgataccga ctttgagcat ctcggactga cgcgccaata cataagtgcg
    11881 tacgtctttg agccattggg tcgaaacttc ttccattttg tcgttgacca gattcaggtt
    11941 caactgaccg gacaggcgta ccgccaaatc cataaacaaa tcgtcgaagg ttttttcgcc
    12001 tgccggagag tgcgggatgt cgagcagcat actgaagcct ttgtaggact ggttgtccaa
    12061 aagggcattg gtaaacggct cgttgttgag cgagcagatg gagaacatgg tcgagcccga
    12121 cgtgtcggta tagtggaacg cgccgtcgtc ttccaaaacg aaacccacgc ccgttacggc
    12181 ggaacgcagt tctacgccgc tgatgctggt cggcgaaacc aaatggatgg cgatggtctg
    12241 gtcgacgcgc gcgcagaatg cgtccagtgc ggaagccact tcgataaagg cggcaaggtc
    12301 ggtgtgcagc gtctgaccgc ccatgctttg tgcgaatgcg tccgcctggc ggttgaatgc
    12361 ggagagttct tcctgcgagg caagtccgtt gcggctgact gcctggatac ccacgataaa
    12421 tgcctgatag cggatgcccg ggatgggttc ggcaatctgg aaatggtcgt ccatggtgca
    12481 gccgacaatc tggtagcggc agcggttgga aaggcgcggc agtgcgtgca gttctttggc
    12541 ttcggtcagc gcgatatagg agatgaagtc gaagcgcacg tcaaaccagg gcagctcgac
    12601 cttcgacagc tctttgagcg taatcagcgg ttttgccggc gtttgcggaa cgggaacggg
    12661 ttttgccggc gcgtcggcag gtttcggtgc ggaatgtccg gtttggggtt cggaaacggt
    12721 gtgggcggag ttgccgataa tgccgatttc ttccaaggcg gtttcgattt cggttttgaa
    12781 cggggaggct tttgcctgtt tctgcttggc gatgtagacg gcatcctgtt cttgcaggtt
    12841 gcgcatggcg gagtcttggg gtttggccgg ttttttgacc gccggttggg gtttcggcat
    12901 catgactggc ccgccggacg gtttgccgtc gcggacatgg ctggttttgc tgttgagcag
    12961 ggcatctttg tcggagtgtc cgaactggtc gcgcactttt ttgcggtatt ggttttcctg
    13021 atacatattg taggcgacaa cggcgaggac ggcggcgagg aacagtacga tgtaaatcat
    13081 ggcaatcact ttaaatttcg ggatgcagga tgcgcaaagt gcgggtactg cggttaaatc
    13141 gggcttgcgt ttccggcagt ctgacggaac ggccggttat aacgtttgaa ttataacgaa
    13201 aattgcaggg tctgacagca gtgtgtcgaa ataagcggaa attttccgaa atgccgtctg
    13261 aaatctgtgg ttttcagacg gcatttctgt ccacgggaaa ccctttttcc cgtatccgcc
    13321 gccagtcgaa aaaatggccg gggtcggttt tgcggccggg tgcgatgtct tggtgtcccg
    13381 ttaccgccgt gatggggtag cggcggcaga gggctgccaa cagcgtttcg agcgaacggt
    13441 attgcgcttc ggcaaacggt tcgaaatcgc agccttccag ttcgatgccg atggaaaacg
    13501 cgttgcactt ctcccgcccc cgaaacgagg atgcgccggc gtggtacgcc atgtcgccgc
    13561 atgatacgaa ctgcaccgtt tcgccgtcgc gtgcaatcag gaaatggctg gacacgtgca
    13621 aagtgtgtat caggctgaag aacggatgtc cgtcggggtc gagccggttg gcaaacagct
    13681 tttccaccgc atccgtgccg tattcgaacg gcggcagcga aatgttgtgc agcacgatca
    13741 gggaaaccgt ttctcccgtt tccctcgggc tgaaattggg cgacggggta tggcgtatgc
    13801 tttgaagcca gccgttttgc cagtgtgctc cggcgtgatt gtccatgatg ttcttcctgt
    13861 ccggcgggca atttgggtta tactgtcgcc cgaattttaa gacgtattcc gaatgctggg
    13921 aatcctacca tgttgaaaaa aatgttgaaa tggactgccg tttttttgac cgtatcggca
    13981 gccgttttcg ccgcgctgct ttttgtccct aaagacaacg gcagggcata ccgaatcaag
    14041 attgccaaaa atcagggtat ttcgtcggtc ggcaggaaac ttgccgaaga ccgcatcgtg
    14101 ttcagcaggc atgttttgac ggcggcggcc tacgttttgg gtgtgcacaa caggctgcat
    14161 acggggacgt acagattgcc ttcggaagtg tctgcttggg atatcttgca gaaaatgcgc
    14221 ggcggcaggc cggattcggt taccgtgcag attatcgaag gttcgcgttt ttcgcatatg
    14281 aggaaagtca tcgacgcaac gcccgacatc ggacacgaca ccaaaggctg gagcaatgaa
    14341 aaactgatgg cggaagttgc gcccgatgcc ttcagcggca atcctgaagg gcagtttttt
    14401 cccgacagct acgaaatcga tgcgggcggc agcgatttgc agatttacca aaccgcctac
    14461 aaggcgatgc aacgccgcct gaacgaggca tgggcaggca ggcaggacgg gctgccttat
    14521 aaaaaccctt atgaaatgct gattatggcg agcctgatcg aaaaggaaac ggggcatgag
    14581 gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc tgaaaatcgg tatgcgcctg
    14641 caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat acaagggcaa aatccgtaaa
    14701 gccgacctgc gccgcgacac gccgtacaac acctatacgc gcggcggcct gccgccaacc
    14761 ccgattgcgc tgcccggcaa ggcggcactc gatgccgccg cccacccgtc cggcgaaaaa
    14821 tacctgtatt tcgtgtccaa aatggacggc acgggcttga gccagttcag ccatgatttg
    14881 accgaacaca acgccgccgt ccgcaaatat attttgaaaa aataaaccat gccgtctgaa
    14941 aagtttgtgt tttcagacgg cataccctta ccggaactgc aagcatgaaa ccgcaattca
    15001 tcactttgga cggcatagac ggtgccggaa aatccaccaa ccttgccgtc atcaaggcat
    15061 ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga gccgggcgga acgccggtcg
    15121 gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa agccggtttg cgtgcggaaa
    15181 cactgatgat gttcgccgcg cgtatgcagc acatcgagga agtcatcctg cccgcgcttt
    15241 cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc gaccttcgcc tatcagggcg
    15301 gcgggcgggg gatgccgtct gaagacattg aaattttgga acattgggtg cagggcggtt
    15361 tgcgccccga tttgaccctg ctgttggatg tgccgctgga agtatcgatg gcgcgtatcg
    15421 gacaggcgcg cgagaaagac cggttcgagc aggagcaggc ggatttcttt atgcgtgtgc
    15481 gcggcgttta tctcgaccga gccgccgcct gtcccgaacg gtacgccgtt atcgacagta
    15541 accgcagctt ggatgaagtc agaaacagca tagaaaaagt gttggacgga catttcggct
    15601 gttgatgcgg caaatattga aacaagcgca tccgcccgcg ccgaaaacca aacggcagtg
    15661 ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat gatagaatta cgctcggtta
    15721 caaggcagga tgcgtcggca atattaacga accgcccgta acatgatgac ccgaaagcgt
    15781 ttcggacagt tcgattcaaa tctttttctc gcaacaggat tgacacatgg aaaactcatt
    15841 gaaagaagcc gccctcaagt tccacgaatt acccgttccg ggcaaaattt ccgttacccc
    15901 gaccaaatct ctggcgaccg acaaagattt ggcgttggcg tactctccgg gcgtagccgc
    15961 cccctgtatg gaaatccatg ccgatccgca aaatgcctac aaatacaccg gcaaaggcaa
    16021 cttggtcgcc gtcatttcca acggtacggc cgttttgggc ttgggcgaca tcggcgcgct
    16081 ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc aaaaaattcg ccggtgtgga
    16141 cgtgttcgac atcgaaatcg acgaaaaaga cccgcaaaaa ctggtggaca tcatcgccgc
    16201 tttggagccg accttcggcg gcatcaacct cgaagacatc aaagcacccg agtgtttcta
    16261 catcgaacgc gaattacgca aacgctgcaa aatccccgta tttcatgatg accagcacgg
    16321 cacggccatc attaccgccg ccgccgtatt gaacgccctg cgttataccg gccgtaaaat
    16381 cgaagaagcg actttggtgt gctccggcgc aggtgccgcc gcgattgcct gcctgaacca
    16441 actgctggat ttgggcttga aacgtgaaaa cgtaaccgtt tgcgactcca aaggcgtgat
    16501 ttaccaaacc cgcgaagaca aagaccgcat ggacgaatcc aaaaaattct acgccgttga
    16561 agacaacggc cggcgcgtac ttgccgatgc ggttaaaggc aaagacatct tcttgggcct
    16621 ctccggcgca aacctgctga cgcctgaaat gttgaacacg atgaacgaaa aacccatcgt
    16681 gttcgccatg gccaacccga atccggaaat cctgccgccg ctggcgaaag aaacccgtcc
    16741 ggacgtggtt atcggtaccg gccgctccga cttcccgaac caagtgaaca acgtattgtg
    16801 cttcccgttc atcttccggg gcgcgttgga tgtcggcgca accaccatca acgaagaaat
    16861 gaaacgcgcc tgcgtgtatg ctttggcaga tttggcgatg gaagaagtaa ccgaagaagt
    16921 ggttgccgct tacggtaaga aattcgaatt cggcgcggaa tacctgattc ctactccgtt
    16981 cgattcccgc ctgctgcctc gcgtcgctac ggctgccgcc aaagcagcga tggaaagcgg
    17041 cgtggcaacc cgtccgattg cagatttgga agcttacgct gccaagttgg gcgaatggaa
    17101 gctgtaagcc gtttgtggtt aaaatgccgt ctgaactgtt ttcaggcggc attttgttgt
    17161 cagattgata aatgaaagat actggaaaat gaaagagatg aaacctgtcc gttatcatat
    17221 tggcgatata cccgaaactt caaaacaaac cgcccccggt catgacgaca gggcagtggg
    17281 tgttgacgat gattgttttc atgattcctt tggtcaatat cgttatgttt tttgtttggg
    17341 cgttcggcag aggcaacccc aaccgtgcca atttctgtaa agcactgttc ttatttactt
    17401 taccttgttg gttcgcttat cggtttgatt ttcatattgc ttataggtgg gtctgcatcg
    17461 ggtacgcatt attaatgtgc cggctgattc tgcttcgaag atttgtatcg aatatgccaa
    17521 atttttttaa atttcatacc gttatcgaac ggcattggca aaaaccttat ccggttttgt
    17581 cttttctgct caagccgctt tccgggctgt ttgccaaaat tgcggcaaaa tggcgggcgg
    17641 attttttatc gggaaaacgg caaagcgaaa agctgtccgt gcctgtggtc gtggtcggca
    17701 acatccacgc gggcgggacg ggcaaaacgc cgattgccgc cgcgctggtg tcgggcttgc
    17761 aggaaaaagg tgtcaaggtc ggcatcatca gccgaggcta cgggcgcaag agcaaggcgg
    17821 tttatgtatt gaatgccgcg agccgtgcgg aagatgcggg cgacgagcct ttgctgctgt
    17881 tccgtaaaac aggtgcgccg acggcagtgg gcagcagccg tgtggaggca ggcagggcgt
    17941 tgctggcagc gcatccggag cttgaattga ttgtggcgga cgacggtttg cagcattacg
    18001 ccctgcaacg cgatgtggaa atcgcggtgt ttccggcggc ggatacgggg cgtacggatt
    18061 tggacttgct gcccaacggc aatttgcgcg aacctttgtc gagattggaa agcgtggatg
    18121 cggtggtggt cggcggcagg gcggcggatg gttttatgcc gtctgaacat ttgttcggca
    18181 gccgtattga ggcgggtgcg gtttaccgtt tgaaccgtcc gtctgaaaaa ctggatattt
    18241 cgacattgtc agggaagcgc gtcgcagcgg ttgccggtat cgccaggccg cagcgtttct
    18301 tcgatactct gacacacatg ggcatccgcc ttgaccaaac ggttgctctg cccgaccatg
    18361 ccgatatttt caatcgggat ttgccgcctg ccgatgtggt gctggtaact gaaaaagatg
    18421 cggtcaaatt ttcagacggc atttgcaccg ataatgtttg ggtgctgccg gtttgtgcga
    18481 taatcgaacc tgatttggcg gagtttgtgc tggagcggtt ggagggtgta ccgaaggccg
    18541 tctgaaagcg cggtttggac gaagtgataa cggactggaa taagaacgcc ctacgccgtc
    18601 attcccgcgc aggcgggaat ctaagtctcg aattttcagg aatgcctagg agacttcaga
    18661 aatcccaaat ctccggattt ccacttgggc aggaatgaga aagccggttg tattttttat
    18721 ctgcattaat cattcgttaa aggagtggat atgaagctga aaaccttgtt attgcccttc
    18781 gccgcactgg cattgtgtgc caacgcattt gccgccccgc ccggcgacgc gtcgttggca
    18841 cgttggctgg atacgcagaa tttcgaccgg gatatagaaa aaaatatgat tgaaggcttt
    18901 aatgccggat ttaaaccgta tgcggacaaa gcccttgccg aaatgccgga agcgaaaaaa
    18961 gatcaggcgg cagaagcctt taatcgttat cgtgagaatg ttttgaaaga tttgattacg
    19021 cccgaagtga aacaggctgt ccgcaatacc ttattgaaga atgcccgtga aatatacacg
    19081 caagaagaaa ttgacggcat gattgccttt tacggttcgc ctgtcggtca gtccgtggtt
    19141 gccaaaaatc cgcgcttaat caagaaatcg atgagtgaaa tagcggtatc ttggactgca
    19201 ttgtcaggga aaatcgcgcg acatcatctg cccgagttta cggaagagtt acggcgcatc
    19261 atctgcggcg gtatagtgga ttaaatttaa atcaggacaa ggcggcgaag ccgcagaccg
    19321 tacaaatagt acggcaaggc gaggcaacgc cgtaccggtt taaatttaat ccactataaa
    19381 aatcccgatg cgggctgcaa gcaggccgga cagcttggga aaaggtatca gaagtaaata
    19441 atagccgcct gaaatattgc ggagggcatc cgattgatta aaccatcaaa cccgaaagca
    19501 accttatgga aaaaaaattc ttagacatcc tcgtctgccc cgttaccaaa ggcaggctgg
    19561 aatatcatca ggacaaacag gaattgtgga gccgtcaggc gaagcttgcc tatccgatta
    19621 aagacggcat tccctatatg ctggaaaacg aagcacgagc gttgagcgaa gaggaactca
    19681 aagcatgacc gaattcgtcg tattgattcc ggcgcggctg gattcgtcgc gcctgcccgg
    19741 aaaagccttg gcggacattc acggcaaacc gatggtcgtg cgcgttgccg aacaggcggc
    19801 aaaaagcaaa gccgcgcgcg tcgtcgtcgc caccgatcat cccgacattc agacggcctg
    19861 tcaggcgcac ggtatcgaag tcgtcatgac ttcaaaccgg cacgaaagcg gcacgacgcg
    19921 ccttgccgaa gccgccgccg cgctgaagct gccgccgcat ttgattgttg tgaacgtaca
    19981 gggcgacgag ccgctgattg cccccgaact catcgaccgc accgccgaag tactcgtcga
    20041 aaacaacgtc caaatggcga ccgccggcca cgaattgcac gatttcgacg aattgatgaa
    20101 tcccaatgcc gtcaaagtcg tcctcgacaa aaacggcaac gccatctact tcagccgtgc
    20161 cccgattccc tatccgcgcg atgcgatgcg tgccggaaaa cgcgaaatgc cgtctgaaac
    20221 cgccgtccta cgccatatcg gcatttacgc ctaccgcgtc ggcttcctgc aacgctatgc
    20281 cgaaatgagc gtctcgccgc tggaaaccat cgaatcgctg gaacaactgc gcgtcctgtg
    20341 gcacggctac cccatcgccg tcgaaaccgc caaagaagcc cccgccgccg gtgtggatac
    20401 gcaggaagat ttggacaggg ttcgcgctgt atttcagacc gtataaaaca ggttcaaagg
    20461 gaaaagatat gcagcaacat attgaaaagt ggcaacactt gagccgtgaa gaacagaaaa
    20521 tccttgccga agtatggggt ctcgtgcaaa acgatgatca ggaggttcac tatgaaatgc
    20581 tcaaattgaa cgcacctgat gaagtcagcg gtgaattttg gttcagaatg gcagaaacac
    20641 tcagcaccct gccgcccaat cgttccctcg accttagaat gaacggcggc aggctgtcga
    20701 ccgccgtatc catcctttcc gtcatgattg aagacaatcc cgacataccg cagctttggg
    20761 cgtaaaaaat taccgccctc aattatttgg tacacggaca caaagcccgt gccgacggtt
    20821 tggtacaaca gcccgacaaa gcggcagaag ccaacgagga ggaatacctg accaaagccc
    20881 tgtcgcaaaa cctgctgtca acattggatg ccgcacttgc acgttttcct gaagacgcgt
    20941 ggtttcagga aatcaaacag gatgcacaaa agcattttgc ttgaggacgt ggcggtcagg
    21001 aatatttcca ttcaggaaga aaagaagtgc ctaattgggt ataatcaggg taaatcttat
    21061 tttatttcaa aagattaata tttgttttcc gtttttcctt gacggtatcg gaaaagttga
    21121 ttatagttac agcttcctta ggagtaatgg ctgagaggct gaaggcactt ccctgctaag
    21181 gaagcatgtg ggatcaacct gcatcgaggg ttcgaatccc tcttactccg ccagataaaa
    21241 aaatagacgc tgtgttttac agcgtctatt tttttatgca attttatagc gggttggcgc
    21301 aaacccggta tggtattgcc ctgtcttgat tctgaatttt gttaatccga gatgtttgcc
    21361 gtttattttt gcctcgttca aacggcggct ctgatctgcg cggtttctgt ttgccgtatt
    21421 cgcctacccg taccgcaaat gttatactgg gaaaaattca ccgattgtat tttacggcgt
    21481 atttgccgat aggatggaag agacaaatga gcagaatccg gcaggctttt gccgctttgg
    21541 atggcggaaa ggcattgatt ccctatattg cggtgggcga tcccgatatt cggacaactt
    21601 tggcattgat gcacggcatg gttgcaagcg gtgcggatat tttggagttg ggcgtgccgt
    21661 tttccgatcc gatggcggac gggccggtta ttcagcgtgc ggcggagcgg gcgttggcaa
    21721 acgggatttc gctgcgcgat gtcttggatg tcgtcagaaa attccgtgaa accgacacgc
    21781 aaacgccggt tgttttgatg ggatatttga atcccataca taagatgggt tatcgggagt
    21841 ttgctcagga agccgcaaag gcgggggtgg acggcgtgtt gacggtggat tcccccatcg
    21901 aaaccatcga ttctttatat cgcgagctga aggataacga ggtcgactgt attttcctga
    21961 ttgcgccgac gacgacggaa gaccgtatta aaaccattgc cgagctggca ggcggatttg
    22021 tctattatgt ttcgctcaag ggcgtaacgg gcgcggcaag tttggatacg gatgaggttt
    22081 cgcgtaaaat agagtatttg cgtcagtata tcgatattcc catcggtgtc ggtttcggca
    22141 tcagcaatgc ggaaagcgcg cgcaaaatcg gccgggttgc cgccgcgatc attgtcggca
    22201 gccggattgt gaaagagatt gaaaacaatg cgggcaacga ggctgccgcc gtcggtgctt
    22261 tggtcaaaga gttaaaggat gccgtgcgct gattctgtcg cgcattctaa atgttttagg
    22321 agttgtccat gagctggtta gataaaatcc tgccacccaa aatcaagaat cgtgggaaag
    22381 acggttcttc caatgttccc gagggtttgt ggcgcaaatg cccgtcttgt tcggcaaccg
    22441 tttattcgac cgagttgcag cagaacaatc aggtctgccc gaaatgcaac caccacaatc
    22501 cgttatcggc gcgccagcgt ttgaacctgc ttttggatga ggaaggcagg gaggaagttg
    22561 cgggtaatgt caaaccgacc gatcctttga agtttaaaga cagcaaaaaa tatccggacc
    22621 gtttgagtgc ggcacgcaag ctgaccgggg aagatgacgc gctggtggtg atgaagggga
    22681 tgatgaacgg tctgcccgtc gtcgttgccg cgtttgaatt ccgctttatc ggcggttcga
    22741 tgggttcggt tgtgggcgag cgtttcgtac agggtgtccg ccgtgcggtt gccgataatt
    22801 gtccgtttgt ctgtgtggcg gcttccggcg gcgcgcgtat gcaggagggt gtaaactcgc
    22861 tgatgcagat gacgaaaacc agtgccgcgc tgcatttgct gacggaaaaa cgcctgccgt
    22921 ttatatcggt gttgaccgat ccgactatgg gcggcgtatc tgccagcttc gcatttttag
    22981 gcgatgtcgt gcttgccgaa ccgaacgcgc tgatcggttt tgccggtccg cgcgtgattg
    23041 agcagacggt gcgcgaaacg ctgccggaag gcttccaacg cgccgagttt ctgctggaaa
    23101 aaggcgcgat cgaccagatt gtcgaccgcc gcgatatgaa gcggcgcatc agtgatttga
    23161 ttacgctgtt gtgccgtcag gacaaagttt ccgccacctg atggctgatg aatcgaatgc
    23221 cgtctgaaac cgatgtttca gacggcattt ttgtgtctgg ttatttgtcg tgcggcttca
    23281 tcgacggggc atagcgtccg gcacgttctt tcaggcgttg taccaaacct tgcgtgtcgg
    23341 cgggtacgcc gccctcgcag aatgcctgat acaggacggc gcgcaatgcg tcgttgcgcc
    23401 caagtgtgcc gcctatcggt ttccattcgg cgtttcgggg ctgtatccag cggctgttga
    23461 ccgtatcgcc gtatccgaat actttgtagg aggaaagttt gccgttgccg aacccgatgg
    23521 aggagagggc gcagaatatg ccttcggcag tcaggttgtc gtagcctttg tcggagcgga
    23581 tattgagaat gtagcggatg ctgccgtcgg gcgcgggcat aatttgcagg ctgtcgagca
    23641 ggattttcgg ctgtttgccg taattttcat ccacataaat gtcaaaccag ccgtccgagt
    23701 gcgcatcggg cagaggcggc agttcggcgg tatgttcttt aaattcgcgg gcggcggctt
    23761 cttcgggcgt ttcgcggtag cgggtgttga tcggcgtgtc tttttggctg aagccggcgg
    23821 caagggacgt gccgacagtc agggtcagaa tcagaaggat ggcgcggcgc ataagtttct
    23881 ccaaattgaa aacggcttta ttttatggtt tggcgggaag ggctgcaagc aatcggggta
    23941 taatctgacc tgattttcat tttaaagcgg tgtcgaacca tgaacagcga aactttagac
    24001 gtaaccggat tgaaatgtcc cctgccgatt ttgcgggcaa aaaaggcttt ggcgcaaatg
    24061 cggcagggcg aggtattgac cgttctggcg accgacggcg gcgcgccggg ggactttgag
    24121 gctttttgcc gccaaaccgg tcatgtgctg ttggattctt ccgaacagga cggcgtgttc
    24181 acactggtcg tcaagcacaa ataaatgccg tctgaaatgc ggatgtcctg attgatgtta
    24241 ttgctgtcgg catttgtgct tgctgcgtgt aatgcccaaa accggcaatc cggtgtacgg
    24301 catcaggatt ttgcacgcgt agccgtcgat ttgtttcgga gtgcctgcgt cctgactcaa
    24361 ggtagtttcg aaccggtttc caagtttgcc gctgtcggac attttgtccc tgtcggaaag
    24421 gaagacctgt cccgtctgcc gcccgctgtt gcagaacccg atgtacaggc gttgtggacg
    24481 cttgaacgcg atggcggcat ttattatttg agcctgacgc gcgacagttg cagtgtgaaa
    24541 gccgagcggg cagacagtgc cgcgcttttg gaacatttct cagggcttgt ccgacagccg
    24601 ccgaaaaatg c
//
LOCUS       DAWTCI010000023        22262 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWTCI010000023 DAWTCI010000000
VERSION     DAWTCI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22262
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.023"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..152)
                     /locus_tag="WM481_000931"
     CDS             complement(<1..152)
                     /locus_tag="WM481_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9080273.1"
                     /translation="MKELKWIEEARKYLGAHEKVNGKSNPVLLAMLQEMGNFNQEQKA
                     WWKETDT"
     gene            359..595
                     /locus_tag="WM481_000932"
     CDS             359..595
                     /locus_tag="WM481_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFA9080274.1"
                     /translation="MLRLQKWGNSAAVRLPARLLTALNAKIGDALETEIRNGELLIRP
                     VKRYRLSDLLAETEAAPPRAEGWEEMPDVGQEGV"
     gene            595..942
                     /locus_tag="WM481_000933"
     CDS             595..942
                     /locus_tag="WM481_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9080275.1"
                     /translation="MYIPERGDIFHLAFDPAAGTEMKGGHYAIALSPRAYNRATGLVY
                     ACPISQGRAAAARSGGMISTLLGAGTATQGNVHCHRMKALDWKIRRAAFRETVPDYVI
                     EDVLARIGAVLFD"
     gene            complement(997..1149)
                     /locus_tag="WM481_000934"
     CDS             complement(997..1149)
                     /locus_tag="WM481_000934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080276.1"
                     /translation="MKFSINREFRKMLKLFAEYAVLRRMFYAVLGAAPLTALIWKPAD
                     ILNALK"
     gene            complement(1310..1642)
                     /locus_tag="WM481_000935"
     CDS             complement(1310..1642)
                     /locus_tag="WM481_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080277.1"
                     /translation="MGFLQFGLLFAAAGGVLGAVWASLQERGRPVQAVLEAFISAIAA
                     AAAAERFVPLDQAWTCAAAGVFAGMMTGHALDTVRALAPKVLRGYLGGLAEKLTGVKD
                     GGSSDGKD"
     gene            complement(1643..2113)
                     /locus_tag="WM481_000936"
     CDS             complement(1643..2113)
                     /locus_tag="WM481_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080278.1"
                     /translation="MLRVLLNRRFLPAGFQAWLFGTATRVLEAVSGLGLSGYAAVFAL
                     APDEIYAWRIYYKFQDIPEAWTVGVLAAAGLLQTALLFARGVRACVASAYLLLFSGFV
                     WFLVSVAFWGAYPPLNTGMVVPPLLAFFCALAGNNALRFLFSAQKSRGLADEGS"
     gene            complement(2184..2489)
                     /locus_tag="WM481_000937"
     CDS             complement(2184..2489)
                     /locus_tag="WM481_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080279.1"
                     /translation="MIEDKKIVALNFAVEDGMTGASAAYHVVEHIGADYRNGYVTATL
                     NGYVSENAFKSGKRYLLARTLDFQETDITAPDPGWVYRKALEGAAGIPKDAQPVYAE"
     gene            complement(2601..6746)
                     /locus_tag="WM481_000938"
     CDS             complement(2601..6746)
                     /locus_tag="WM481_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFA9080280.1"
                     /translation="MGGKSSTVTSAEERILSLQVQRSSQGLTLPVIYGRTRVAGNLVW
                     YGDFVTIEHKTTTRRGGKGGGGVKQVGISYTYEAAVMLALCEGEIQGVGRVWRDKEKF
                     DSPAQLRLTLMRGGDEQPLWTHLQQAKHQGQALNYSGTAYLCSPNYELTKSAQIYQHN
                     FEVIGKSGYSGNIPDANPREIVLDLLTNQRYGCGFPSQNIGDTDRYSNYCRAVGIFLS
                     PAYTEQGEAQRNISELLEQTNSAAVFSQGRLKIIPYGDGSHSGNGAVYVADNKAAYDL
                     TDDDFIVSGAQDPVKAGRKTNADAFNQIQVEYLDRDNDYNVAIAEAKDQANIEQYGLR
                     PKDAVRMHGICDAKVAQKVAQQLLQRALYVRNEYEFKLGWKYCLLEPMDIVTLTDAGL
                     GLNKTPVRITEIEEDGEGVLSVKAEDCPAGVYTVSEYPTQPSSGYSADYNVSPGNAHV
                     PVIFEAPLQLTGGEPQIWLATAGGGMWGGAEVWVSADGDSYTRVGAVNRKARFGALTA
                     DLPDGAVFDRTNTLGVEISAGQLTGGTEQDSRDLLTLCYVDGEFLAYADAELKGVGRY
                     TLGNLTRGAYGSAVNAHAAGSRFARIDEALFKYAVPRNWIGRTVWVKLVSYNVFGGGI
                     QDLAEVPAYSYTIEGAPLGQIQNLRLTSSWAYGKEAVIAWDKLDGADTYDVEIYAGGS
                     RRRLRAVDGIVDNSYTYTQADMKADGGQVRGIVFKVRGRAVTGKTGNWAQIAAQNPQL
                     QALQGISIDSGLRQAFFTCQKPDEEDFAGIIVWVCENAACPAADANKAYDGAETFITI
                     AKCGGKPLEKGKTYYLRAAGYDSFGKDNLKISGSISFTVYDVSATDLSESSLNKALRD
                     KINLIDGNGAGSVNERVEAVRSTADGNAAAVQTHARSINGLEAQYTVKVDANGKVAGF
                     GLATTPKNGTPESKFIVNADRFGIGAAGKADVFPFVVDTQKNRVGINGELVVNGKAVV
                     DKLNAGDIHGGKIAADTLDANRLKAGSVTAREIGAAAVTADKIGANAVTADKIQVADL
                     SAVSSNLGSITGGSLNIGGGNFTVSSDGILTADNAVIRGRIEADSGYFNGTVRASSVE
                     GDVLRAHRLRWTEGNVWVLDLDKDPLPRVLIPNFYVVSETFGNNRVQAKLLLNGGVLA
                     PREVRETENYTNYIWRGRTFGRYEDLPPRARGGGDYTEQSGSRYRTRLEYQIQVIPAG
                     KPVSLKLSLASHESVFSPFVSVSYLAQSDYEYKQLLGRMVWRTFAESFRYDSKKQVYL
                     GGDRRVHDYQNQMYKHWNAYGGLLQLPDDIYGISFEYRPFTNADWSTMLAFDRSDKFV
                     VVKKYRGHAPQQYSLFQEEFNTAVPKSNLLFFVEHWWQYIELRNIRVLIPESRENEVW
                     QVG"
     gene            6986..7264
                     /locus_tag="WM481_000939"
     CDS             6986..7264
                     /locus_tag="WM481_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFA9080281.1"
                     /translation="MESQTFASVFDALCDTPAQAANMRLRAGLMMHIADTVRENGWTQ
                     KQAAEHCGLTRPRINDLLNGKIDKFSLDALVNINAGLGQCISLSFAPA"
     gene            complement(7292..7723)
                     /locus_tag="WM481_000940"
     CDS             complement(7292..7723)
                     /locus_tag="WM481_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9080282.1"
                     /translation="MPSETDLRARIVEEARSWLGTPYHHHAMVKGAGVDCAMLLVAVY
                     GAVGLLPEGFDPRPYPQDWHLHRDCERYLGFVTQFCRETESPQAGDIAVWRFGRSFSH
                     GGILAGGGKVIHSYIGRGVVSDDIGQAELIGRGVRFFTFSF"
     gene            complement(7725..8579)
                     /locus_tag="WM481_000941"
     CDS             complement(7725..8579)
                     /locus_tag="WM481_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFA9080283.1"
                     /translation="MKAATKELIDLLHGGDEFQMADLYTITLSGGRVLRHTGADMPVV
                     WDGQAYGAHELVIKRGATRTAVGLEVDSNTLQISAAPDYRLEGLQWAEAALGGVLDGA
                     RVKIDRVFFDAGLRPVGAVNIFSGRVSDVSGGRSSVKVDVKSDIELLNVSSPRNIYQA
                     GCMRTLYDDGCKVNREKFTVNGRVTENSRTGTVLKHNLTQPDGWFSQGVIKFAGGRNA
                     GLSRTVKAHGGNTFELALRLPYPPQAGDAFKVYPGCDKRRDTCKDKFDNIVHFRGFPF
                     IPSADTVV"
     gene            complement(8576..9175)
                     /locus_tag="WM481_000942"
     CDS             complement(8576..9175)
                     /locus_tag="WM481_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HFA9080284.1"
                     /translation="MGNAVFPEFPGLKWGRKKTAVWSTGTQKSASGREFRTAYYTYPQ
                     WRFSLSFEVLRTKASVNELEKLAGFFNARKGSFESFLYEDPADNAVTDQPVGNTVQGV
                     ARYQLVRSMGGFIEPVSAVKERPAVKVGGTALAYGRDYTVTDKGVLVFNTPQPPGRPI
                     TWTGGFYFRVRFTSDTVDFENVLGSLWAAKKIEFTSVKL"
     gene            complement(9175..9441)
                     /locus_tag="WM481_000943"
     CDS             complement(9175..9441)
                     /locus_tag="WM481_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080285.1"
                     /translation="MIAHVCASTGWTWDYVADNLDLPRIKHLNEYWREHPPVHILAAS
                     YMGVKPSSGSVQSEADEAEAVGMLGGGELSEDEFDALLKAKGII"
     gene            complement(9453..9782)
                     /locus_tag="WM481_000944"
     CDS             complement(9453..9782)
                     /locus_tag="WM481_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080286.1"
                     /translation="MTVRIKGVTVELNGADYVIPPIALGALEQLQERIGSFDGNAADA
                     GQISTVIDCAHAALKRNYPDLTREEAADLIDIGNMNEVFAAVMDVSGLKRKEQEAAQA
                     GEARAAD"
     gene            complement(9843..10616)
                     /locus_tag="WM481_000945"
     CDS             complement(9843..10616)
                     /locus_tag="WM481_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080287.1"
                     /translation="MQLTFGSGEVFAQMITDAYGNRVQNATPVRIMGLQEMSVDLSAE
                     LKEFYGQNRYPLAVAQGKVKVSGKMKGALINGLTLNTLFFGTEYATGTMKALWAETTG
                     KVLDGDNYSYLQAAAPGGGKFAEDAGVMGQDGTAYIKVASSPKQGQYTVSESGVYAFN
                     SSDKGKTVYPSFTYTQTMPPAKKIELTNMATGNTPTFKMRYLTQFKGKKALLELESVT
                     SGKLGLFSTKNDDFSVPEIDFTASTDEAGFKIGTLWIQE"
     gene            complement(10642..11070)
                     /locus_tag="WM481_000946"
     CDS             complement(10642..11070)
                     /locus_tag="WM481_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080288.1"
                     /translation="MNREAVYSALWAKLDALDGFVTKSRKLVHWNDVKRYDQPALFMA
                     QGDMQALTLTGRETKWILRADVYLYVQTAGQPPAPVMNPLIDAVCNAVNAVHPVTGKT
                     DLTADGADIEYCRVEGTVETDEGTLGEQAVCIIPIVICAA"
     gene            complement(11067..11549)
                     /locus_tag="WM481_000947"
     CDS             complement(11067..11549)
                     /locus_tag="WM481_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFA9080289.1"
                     /translation="MLKMEFIGGGILAAVFKAYAADVQDAVVKSVGRSALRLQREVKQ
                     NRLSGRVLRVRTGNLRRSVHQRVNVSGNVVSGEVDTNVRYGIAHEYGFAGSVNVKASL
                     RQVRQAFGKPLKPPRYVRVRAHTRDVKLPERSFLRSALRDLTPKFADDLQKSIGKVLK
                     "
     gene            complement(11549..12079)
                     /locus_tag="WM481_000948"
     CDS             complement(11549..12079)
                     /locus_tag="WM481_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFA9080290.1"
                     /translation="MAAPVSLEEFKQRIGVEHDRRDDFFLSVIDGVSAAAEAYIGRSL
                     LAADYVGRYDGNGKDRIVLDNYPVLSVSSVKINGADAGGWEFDNWLLMRPEGFARGLK
                     NVEVSYRAGYERMPADIREAVMIIAVQRVNEIEGKGVRSKTLAGETVAFSTFGNSGGM
                     PPSAFAILNEYKRKGV"
     gene            complement(12082..12414)
                     /locus_tag="WM481_000949"
     CDS             complement(12082..12414)
                     /locus_tag="WM481_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080291.1"
                     /translation="MPSENQTAFSFGDFEMTVKLKAPEGFTDVSFGSQSYAADENGIV
                     EVPSEAAEFLYQFGFGNVASEPAEGPEKAKRGRKPKTGQPAGQSEPAEAAEPAEAEAE
                     PAEAEKAE"
     gene            complement(12421..13920)
                     /locus_tag="WM481_000950"
     CDS             complement(12421..13920)
                     /locus_tag="WM481_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080292.1"
                     /translation="MNVNQLTQETIELMKSAQAGGGPPDKGFTQPAGFTAGLQTYDLS
                     APSQKLYPVLTPLRNRIPRVGGGRTIGSNWKAVTNINVGNQRAGIGEGRRGGVINHET
                     VERNAQFRAIGLENQVTFEADYAARGFEDVKALAVAQTLQATMVAEEMILLGGNTSLK
                     AGVTPTPTAVVSADAAGKLGVSTLSVICVALGLQAYWDVAGANNGATGQSPNIKTAQV
                     PAKITRRNADGTTDTFGGGSARKSAAASVSGIGAGKKVTAVIPAVRGAVAYAWYWGAA
                     GSEKLGAVTTAAKVEISADAEGTQTAASLPSEDNSTSILEFDGLLTQIALPDSGAFWS
                     DNKGGGLTSDNAGGVYEFEEAFAHFFTRYRLSPDTVYVNARDLAALTKLIIGNSGAPL
                     IKLNVDVNNTANIRAGVVVGSYMNKITGDDLNIVVHPNLPAGTYLFYSSRLPAYVQGV
                     GNLLQVRTRQEYYQIEWPLRTRMYEYGVYADEVLQGMFMPAFGMITNVG"
     gene            complement(13961..15118)
                     /locus_tag="WM481_000951"
     CDS             complement(13961..15118)
                     /locus_tag="WM481_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFA9080293.1"
                     /translation="MTKLYAQIAKTEAQDDGTVKVWGYASSEAVDSDGEVVAAEAMKA
                     AIPDYMKFGAVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVTKVKTGVYKGFS
                     IGGRVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKPKDEAGAADKDGKP
                     SDKPTEEEDENPKDGDKGPKTEDKGDKDAGKKDEAGKSASVELSESEIAALKAVLAKA
                     DKPKGGTAAKSMYQVKSPADVLMSLKWLVEDASYDNIDEAVTAQIKESAAGLAESLKA
                     LAAREADKPADGLAAKAGKSGDLAKAESADELAKAQDALKKSNDALAKAQAEIESLKK
                     QAVPPKGSTKAISKAEDNGEDPLKGFQPIVKNDGTLDDVATLIKAKQTGRL"
     gene            complement(15186..17333)
                     /locus_tag="WM481_000952"
     CDS             complement(15186..17333)
                     /locus_tag="WM481_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFA9080294.1"
                     /translation="MSKKTPLSQGFIARVAAGVRYAFTGNADGWFDAGEPLAPVAQQA
                     EGRRFDYEPFYNVGHSKPREREAVGFAQLRALADNYDVLRLVIEARKDQMECLKWTIQ
                     KRDVESTEDDESQRKDRKVDEAVAFFRSPDKEHTWADWLRILLEDLFVIDAPCIYPRK
                     TLGGGLYALEVMDGATIKRVLDNTGRMPLPPDTAYQQILHGMAAVDYTADELIYRSRN
                     NRSYKVYGYSPVEQIIMTVNIALKRQVHALEYYTAGSVPDALVGVPETWSADDIRRFQ
                     EYWDLLLSGETAQRRKMRFVPGELSRNFRETKQPPLKDVYDEWLARVVCFAFSVEPTP
                     FVAQVNRSVAETSREQSLSDGMGSLKNWVKALIDDVLARYMDMAAYEFVWKGEESLNP
                     KEQAEIYAIYKNAGILTADEIRAELGKEPLPGQGQPEPDKQDGRKPEEPPNQEAEKLG
                     KSESPMSEDESAALIEAYLLTRIDGLAEQIAALIEGAAVDWQAGDLAAELSRAAGVVA
                     NGLDFGDWSGLSDVVEPIIRRVAEDGAVAALLRVMPEPAAGMVTNIRSRAVKWAHERA
                     AEMVGMKRAGGGLVRNPAAEWQITEGTREMIRAQVAEAMRNGDSVQELAGRLKESHAF
                     GNARARTIARTETAMADGMGNLIGWEETGLVAGKQWITAKDDKVSDVCNANGGMGVIG
                     LHEPFSHGALTIPGHPNCRCAVVPVLAGDMPES"
     gene            complement(17330..18751)
                     /gene="terL"
                     /locus_tag="WM481_000953"
     CDS             complement(17330..18751)
                     /gene="terL"
                     /locus_tag="WM481_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFA9080295.1"
                     /translation="MALGQFDDVETSVIRSLSSASLYMFTRRMFYQRRGYVWQRANHH
                     APICNALERVFNGETKRLIINIPPRYSKTEIAVVNFIAWAMGRVPDCEFIHASYSAAL
                     AVNNSVQIRNLVQHEEYRAIFPDLALAGESGHHWKTTAGGVMYATGAGGTITGFGAGK
                     HREGFGGCIIIDDPHKADEARSEVRRQNVIDWFQNTVESRKNSPDTPIILIMQRLHEK
                     DLAGWLLDGGNGEEWEHLCLPAIQDDGTALWPEKHDIETLHRMEQAAPYVFAGQYLQK
                     PAPPDGGTFKPDNLQFVKALPAGNIRWVRAWDLASTANDGDYTAGGRLGVTEDGRYII
                     ANIVRGQYGADERDRILRNTAQKDGVKTKISIPQDPGQAGKSQTLYLTRQLAGFSVSA
                     SPESGDKVTRAEPFAAQVNIGNVMVLDDGTWGTDALIAEMRMFPNGQHDDQIDCLSRA
                     FGELLDTRTGMIDFLQSQVEAMK"
     gene            complement(18813..19262)
                     /locus_tag="WM481_000954"
     CDS             complement(18813..19262)
                     /locus_tag="WM481_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080296.1"
                     /translation="MSGTKRKLGRPTDYTKDMADKICEKIANGRSLRSICAEDGVPPM
                     KTIYRWLEANEEFRHQYARAREKQADYFAEEIIEIADSAQAESAAVSKAKLQIDARKW
                     AASKIAPKKYGDKTELDVKSGDGSMRAAVRLDAEEYRKIAEDVLRRV"
     gene            complement(19555..20424)
                     /locus_tag="WM481_000955"
     CDS             complement(19555..20424)
                     /locus_tag="WM481_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080297.1"
                     /translation="MNVIQSFNFNTTSPVRAFEKDGLTWFVAADVCKALEIQNPTQAL
                     EKLDDDERSMFNIGRSEIHGGGGNVNIINESGLYILILRSRKAMEQGSTAWKFRKWVT
                     SEVLPTIRKTGGYQVGQKTTADDRTGLRQAVAALVGRKGIDYSSAYSMVHQRFNVEAI
                     EDIPAGKLPEAVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALYDLAVAVSYG
                     ARAIQMGRDVSLPLKQLGCRQAVTMWTVWAETRSRLKAAANALEALSAHADAEHAEKI
                     RPILPEIRNLSAV"
     gene            complement(20703..21662)
                     /locus_tag="WM481_000956"
     CDS             complement(20703..21662)
                     /locus_tag="WM481_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080298.1"
                     /translation="MAYINILAGDFHKGKAVLKKDCIVLSRGGKVALLDIAGYEVQDG
                     GVIEVVFFDGRRMLVEKNDAFLQAVKVALYNEPQNPEERRQQYNQRQVNSAAKAKKAK
                     KIKLIVGGIFVLLFVAMCAVPKKELTPEEKAAAEKQKIDGKIASGVREFKYDKKAYPK
                     LYKQWGEKAVNEMNGYLPRIAEHIAREDSCDAVETVDISDSRSNPKAKQMVFFVDCRN
                     GKRFFVSTDDLNSGRKSTAEQDKKIDSSAVISQCDEAIKAQLNHPRTFDPHILDTATG
                     VNQNGNVLVTRGFTAKNGLGMQIDYRAYCVITDNKVEVSIEQK"
     gene            complement(21723..22130)
                     /locus_tag="WM481_000957"
     CDS             complement(21723..22130)
                     /locus_tag="WM481_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080299.1"
                     /translation="MNQQEFEFMNDLARAFERRYRDTRSLNRCLSIEGRYMGEEACPH
                     KPEIGLRYGEDAMFLTLQAWAKVDAPQQEAVRISFGIGAKSQAAYEERLQAEIRRRGE
                     GPLHLQTDLGLAAWYGAIRQAAGDDFDLLFEKV"
BASE COUNT         4709 a         6904 c         5563 g         5086 t
ORIGIN      
        1 gtatctgttt ctttccacca cgctttctgc tcctgattaa aatttcccat ttcttgcagc
       61 atcgctaaaa gcacagggtt tgacttgcca ttcacttttt cgtgagcgcc aaggtattta
      121 cgggcttctt caatccattt caattctttc atttcttttc cttccgataa aagacgagat
      181 gtaaactttc ggtttacacc ccggccaagc ctttgattgc aggtttccgc aacaccgata
      241 catccggttt cacacgttcc gtttccatct ccactcctgt tacacgccgc cgcttggcaa
      301 aatccgtttc aggcggcata atgtatcgac aacgtatcta caacacgaaa gcaaaaccat
      361 gttgcgactg caaaaatggg gcaacagcgc ggcagtccgt ctgcccgccc gcctcctgac
      421 cgccctcaac gccaaaatcg gcgacgcgct ggaaacagaa atccgcaacg gcgagctgct
      481 catccgcccc gtcaaacgct accgcctgtc cgaccttttg gcggaaacgg aagccgcccc
      541 gccgcgcgcg gaaggctggg aagaaatgcc cgatgtcgga caggagggcg tttgatgtat
      601 attcccgagc gaggcgacat tttccatctt gccttcgacc ccgccgccgg tacggaaatg
      661 aaaggcggcc attacgccat agccctgtcg cccagagctt acaaccgcgc aaccggcttg
      721 gtttacgcct gcccgatttc acaaggcagg gcggcggcgg caagaagcgg cggtatgatt
      781 tccacactgc tcggcgcggg aacggcaaca caaggcaatg tccactgcca ccggatgaag
      841 gcattggatt ggaaaatccg ccgcgccgcc ttccgcgaaa ccgtccccga ttacgtgatt
      901 gaagacgtat tggcacgcat cggcgcagta ctgttcgact gacttcagag tgcgaagcct
      961 gtccgcaggg ggaagcatcc gtttccatac ccgcccctat ttcaaagcat tcaaaatatc
     1021 cgccggcttc caaatcaacg ccgtcaaagg cgcggcgccc aaaaccgcat aaaacatacg
     1081 gcgcaacacc gcatactcgg caaacagctt caacatcttg cgaaactccc tgtttatgct
     1141 aaacttcaaa cagatttctc cttacctcgg ataaggttaa attcagaaac gccgcaggga
     1201 ttccgcccct cgcggcgttt cgccgttttc aaacctgccg cgtaatgtcg tgcgtcagcc
     1261 ggacgcgccc ggaacacagc gtcttcacgc gccgcgatac cgctcgaaat caatcttttc
     1321 catccgaaga ccctccgtct ttcacacccg taagcttttc cgccaagccg cccagataac
     1381 cgcgcaaaac cttgggcgcg agcgcgcgca ccgtatcgag cgcgtgcccc gtcatcatcc
     1441 ccgcgaacac ccccgccgcc gcgcacgtcc acgcctgatc cagcggcacg aaccgctccg
     1501 ccgcggccgc cgccgcgatt gcagaaataa aagcctccag cacagcctgc acggggcggc
     1561 cgcgttcctg aaggcttgcc cacaccgcgc ccagcacgcc gccggccgcc gcaaacagca
     1621 gtccgaactg caaaaacccc attcacgacc cctcatccgc caaaccccgc gacttttgcg
     1681 ccgaaaacaa aaacctcaac gcattattcc ccgccagcgc gcagaaaaac gccaacagcg
     1741 gcggaacgac catacccgtg ttcaaaggcg ggtacgcccc ccaaaacgcc accgaaacca
     1801 aaaaccacac aaagcccgaa aacaaaagca ggtaggccga agccacacaa gccctcacgc
     1861 cccgcgcaaa cagcaatgcc gtctgaagca gccccgccgc cgccagcacc cccaccgtcc
     1921 acgcctccgg aatatcctgg aatttgtaat aaatccgcca cgcgtaaatc tcgtcgggcg
     1981 caagcgcgaa caccgccgcg taacccgaca agcccaaacc gctgaccgcc tccagcaccc
     2041 gcgtcgccgt gccgaacaac cacgcctgaa acccggcggg caaaaaccgc cgattcaaca
     2101 aaaccctcaa catacccgtc acacaccccc cggcaaaaca ccgcgaagcc gaagccccgc
     2161 agtgtccgac gccgtctgaa aacttattcc gcgtaaaccg gttgcgcgtc tttggggatg
     2221 cccgccgcac cttccaacgc cttgcggtac acccaaccgg ggtcgggcgc ggttatgtcc
     2281 gtttcctgaa aatccaatgt gcgcgccaac aggtagcgtt tgcccgattt aaacgcattt
     2341 tccgacacat agccgttcaa cgtcgccgta acataaccgt tgcggtaatc cgcgccgata
     2401 tgttcgacga cgtggtaggc cgcactcgcg ccggtcatac cgtcctcgac tgcaaaattc
     2461 aaggcaacaa tctttttatc ttcaatcatt tcaaatctcc aaaagcccgc taggggcggt
     2521 aacgaaaatg ccgtccgagg tcttcagagg gcattgcccg gacaaaacag gaaaaaccgc
     2581 agagccggca tacgggggcg tcagccaacc tgccaaacct cgttttcgcg cgattccgga
     2641 atcagcaccc tgatattgcg cagctcgata tactgccacc agtgttccac aaaaaacagc
     2701 aggttggatt tcggaacggc agtattaaac tcctcctgaa acagagaata ctgttggggc
     2761 gcatgacccc ggtatttctt aaccactaca aacttatccg acctgtcgaa cgcaagcatc
     2821 gtactccagt cggcattggt aaaaggccgg tactcgaacg atatgccgta gatatcgtcc
     2881 ggcaactgaa gcagcccgcc gtaagcattc caatgtttat acatctgatt ttggtaatcg
     2941 tgtacacgcc ggtcgccgcc caaataaacc tgcttcttac tgtcgtaccg gaaactttcc
     3001 gcaaaggtgc gccaaaccat cctcccgagc agctgcttgt attcgtagtc ggattgcgcc
     3061 aaatacgaaa ccgacacgaa cggcgaaaac accgattcgt gggaagcgag cgacaacttc
     3121 aggctgacgg gtttgcctgc ggggatgact tgaatctggt actcgaggcg ggttctgtac
     3181 cttgagccgg attgttcggt ataatccccg cccccccttg cccggggggg caggtcttcg
     3241 tacctcccga aagttcggcc gcgccaaata taattggtat agttctcggt ttccctgact
     3301 tccctcggcg caagcacacc cccgttcaac agcagctttg cctgcaccct gttgttgccg
     3361 aacgtttccg aaaccacata aaaattcgga atcaaaaccc tcggcagcgg gtctttatcc
     3421 aaatccaaca cccaaacgtt accctccgtc caacgcagcc tgtgcgccct caacacgtcg
     3481 ccctcgacgg acgaagccct gaccgtgccg ttgaaataac ccgaatcggc ctcaatccgt
     3541 ccgcgtatta ccgcattgtc ggccgtcagg atgccgtctg aagacaccgt aaaattaccc
     3601 ccgccgatat tcaggctgcc gcccgtgatg cttccgagat tggaagatac cgcgctcaaa
     3661 tcggcaacct gaatcttatc ggcagtcacc gcgtttgccc cgattttatc ggcggtaacg
     3721 gcggcagccc cgatttcccg cgccgtaacg cttccggctt tcaggcggtt tgcgtccagc
     3781 gtgtccgccg cgattttgcc gccgtggata tccccggcgt tcaacttatc gacaaccgcc
     3841 ttgccgttga ccaccaattc gccgttgatg cccacgcggt ttttctgcgt gtccaccacg
     3901 aacgggaaca catccgcctt cccggccgca ccgatgccga agcggtcggc attcacgata
     3961 aacttgcttt cgggcgtgcc gtttttcggc gtagtcgcca agccgaagcc cgccactttg
     4021 ccgtttgcat caaccttgac cgtgtattgc gcctccaagc cgttgatgct tcgggcgtgt
     4081 gtctgaaccg ccgccgcatt gccgtccgcc gtcgatcgga ctgcctcgac gcgctcgttg
     4141 acgctgcccg cgccgttgcc gtcaatcagg ttgattttgt cgcgcaaagc cttgttcaga
     4201 ctgctttccg acaggtcggt tgccgatacg tcgtaaacgg taaacgatat gctgccgctg
     4261 attttcaggt tatctttgcc gaaactgtcg taacccgccg ccctcaagta ataggtcttt
     4321 cctttctcca gcggtttgcc gccgcatttg gcgatggtta taaacgtttc cgcgccgtca
     4381 taggctttgt ttgcgtccgc ggccgggcag gctgcgtttt cgcaaaccca gacgataatc
     4441 cctgcaaagt cttcttcgtc aggtttttgg caggtaaaaa acgcctggcg caggccgctg
     4501 tcgatagaga tgccctgcaa tgcctgcaat tgcggatttt gcgccgcgat ttgcgcccaa
     4561 ttgcccgttt taccggtaac ggcacgcccg cgaaccttga agacaatacc gcgtacctgg
     4621 ccgccgtccg ccttcatatc cgcctgagtg taggtgtagc tgttgtcaac gatgccgtca
     4681 accgcacgca gacggcgtcg gctgccgcct gcgtagattt ccacgtcgta ggtatccgcg
     4741 ccgtccaatt tatcccaagc gatgacggct tctttgccgt atgcccacga tgatgtcagg
     4801 cgcaggtttt ggatttgacc gagcggcgcg ccttcgatgg tgtaggaata cgccggcact
     4861 tccgccaaat cctgaatgcc gccgccgaaa acgttgtacg aaaccagttt gacccaaacc
     4921 gtgcggccaa tccagttgcg cggcacggcg tatttgaaca atgcttcgtc gatgcgcgca
     4981 aaccggctgc ccgccgcgtg tgcgttgacg gcggagccgt acgcgccgcg cgtcaggttg
     5041 cccaatgtgt agcgtcccac gcccttcagt tcggcgtcgg cgtatgccag aaattcgccg
     5101 tcgacgtagc acaatgtcag caaatcgcgg ctgtcctgct ccgtgccgcc cgtcagctgc
     5161 cccgccgaaa tttccacgcc caacgtattt gtgcggtcga aaaccgcgcc gtcgggcaaa
     5221 tcggcggtca gcgcgccgaa acgcgccttg cggttgaccg cgccgacgcg ggtgtagctg
     5281 tcgccgtccg ccgataccca cacttcggcg ccgccccaca taccgccccc ggcggttgcc
     5341 agccagattt gcggttcgcc gcccgtcagt tgcaacggcg cttcgaaaat taccggcaca
     5401 tgggcgttgc ccggcgaaac gttgtagtcg gccgaataac ccgaagacgg ctgcgtcgga
     5461 tattccgaca cggtataaac accggccggg cagtcttcgg ccttgacgga taaaacccct
     5521 tctccgtctt cttcgatttc cgtgattcgg acgggcgttt tattcaggcc gagccccgcg
     5581 tcggtcaggg ttacgatgtc catcggctca agcaggcagt atttccaacc cagcttaaac
     5641 tcatattcgt tgcgcacgta cagggcgcgt tgcagcagtt gttgcgccac tttttgcgcc
     5701 accttcgcat cgcaaatgcc gtgcatcctg accgcgtctt tcgggcgcaa tccgtactgc
     5761 tcgatattcg cctggtcttt cgcttcggcg atggcgacgt tgtagtcgtt gtccctgtca
     5821 aggtactcga cctgaatctg attaaacgca tcggcattgg ttttgcgccc ggcctttaca
     5881 gggtcttgcg cgcccgaaac gatgaaatcg tcatcggtca ggtcgtaggc ggccttattg
     5941 tcggcaacgt acaccgcgcc gttccccgaa tggctgccgt ccccgtaggg gatgattttc
     6001 aggcggcctt gcgaaaatac cgccgcgctg ttggtctgct ccagcagttc ggaaatattc
     6061 cgttgcgcct ccccctgttc cgtgtaggca gggcttagga aaatcccgac ggcgcggcaa
     6121 taattgctgt accggtcggt atcgccgatg ttttgggacg ggaaaccgca gccgtagcgt
     6181 tggttcgtca gcaaatccaa tacgatttcg cgcgggtttg catcggggat gttgccggaa
     6241 tagcccgatt tcccgatgac ctcgaaattg tgctgatata tttgcgccga tttcgtcagt
     6301 tcgtagttcg ggctgcacaa ataagccgtg cccgaataat tcaaggcttg gccttggtgc
     6361 ttcgcctgtt gcaggtgcgt ccacaacggc tgctcgtcgc cgccgcgcat aagcgtcagg
     6421 cgcaactgtg ccggcgaatc gaatttctcc ttgtcgcgcc aaacccgccc cacgccctga
     6481 atctcgccct cgcacaaagc aagcatgacg gcggcttcgt aggtatagga aataccgacc
     6541 tgtttcacac cgccgccgcc cttgccgccc cggcgcgtcg tagttttatg ctcgatggtg
     6601 acgaaatcgc cgtaccacac caaattaccg gctacacgcg tcctgccgta gatgacgggc
     6661 agggtcagcc cctgtgatga ccgctgaacc tgtaacgata aaatccgctc ttcggcagat
     6721 gtaacggttg acgatttacc acccataaaa ccacctcgaa acttgcaaaa attatcaaaa
     6781 tcgatataat ttacttatat caaaacagat ataaaatatg aaaccgttaa atttttcagg
     6841 cggttcattg gattgcctgc ggcaattccc cgaaaacgcc aaacaggcgg caggttatca
     6901 acttcaagaa aagccggaaa accgcgggcg ccgattcgga atcggcggaa aaacgttaca
     6961 acaaattgat tcgggaaaag aaataatgga aagccaaact ttcgcctccg tatttgacgc
     7021 actgtgcgac acgcctgccc aagccgccaa tatgcggttg cgcgccggcc tgatgatgca
     7081 catcgccgat accgtccgcg aaaacggttg gacgcaaaaa caggccgcag aacattgcgg
     7141 cctgacccgg ccgcgcatca acgacctgct gaacgggaaa atcgacaaat tttcattgga
     7201 tgcgctcgta aacatcaatg ccggactcgg gcagtgcatt tccttatcct tcgcccccgc
     7261 gtaaagcccg tcctttcaga cggccgcaaa atcaaaatga aaatgtaaaa aaccgaaccc
     7321 cgcgcccgat aagttcggct tggccgatgt cgtccgacac cacgccgcgc ccgatgtagc
     7381 tgtgaataac cttgccgccg cccgccaata tgccgccgtg cgaaaacgac cgcccgaaac
     7441 gccatactgc aatgtcgccc gcctgcggcg attccgtttc acggcaaaat tgcgtgacga
     7501 accccaaata acgctcgcaa tcgcggtgca gatgccaatc ttgagggtaa gggcgcgggt
     7561 cgaacccttc gggaagcagt ccgaccgccc cgtagacggc gaccaggagc atcgcgcaat
     7621 caacacccgc gcccttgacc attgcatgat gatggtacgg cgtgccaagc cacgaccgcg
     7681 cctcttcgac gatccgcgcc ctcaaatccg tttcggacgg catatcacac caccgtatct
     7741 gccgaaggga tgaacgggaa gccgcggaaa tgcacgatat tgtcaaactt atccttgcag
     7801 gtatcgcgcc gcttgtcgca gcccggataa accttgaacg catcgcccgc ctgcggcggg
     7861 tagggcaggc gaagggcaag ttcgaacgtg ttgccgccgt gtgccttgac cgtcctgctc
     7921 aaacccgcgt tgcgcccgcc cgcaaactta atcacgccct gcgaaaacca gccgtcaggc
     7981 tgcgtcagat tgtgcttcag cacggttccc gtccggctgt tttcggttac gcgcccgttt
     8041 accgtgaatt tctcacggtt gaccttgcag ccgtcgtcat agagcgtcct catgcagccc
     8101 gcctgataaa tgttgcgcgg gctggaaacg ttcaaaagct cgatgtcgga tttcacgtca
     8161 accttcacgg acgacctgcc gcccgatacg tccgacacgc gccctgaaaa aatattcacc
     8221 gcaccgacgg ggcgcagtcc ggcatcaaaa aacacacggt ctattttgac ccgcgcgccg
     8281 tccaatacgc cgcccagggc ggcctccgcc cattgcaggc cctcaagcct gtaatcgggc
     8341 gcggctgaaa tctgcagggt gttggaatcc acttccaatc cgacggcggt acgggttgcg
     8401 ccgcgcttga taaccagctc gtgcgccccg taggcctgac cgtcccaaac gacgggcata
     8461 tccgcgccgg tatgccgcag cacccggccg cccgaaagcg taatggtgta caaatccgcc
     8521 atctgaaact cgtcgccgcc gtgcagcaaa tcaatcagtt cttttgtcgc cgccttcata
     8581 gtttcacact cgtaaactca atctttttgg ccgcccacaa gctgcccaaa acgttttcaa
     8641 aatccaccgt gtcagacgta aacctcacgc ggaaataaaa gccgcccgtc catgtaatcg
     8701 ggcggcccgg cggttgcggc gtgttgaaaa ccaaaacgcc cttgtcggta acggtgtaat
     8761 cgcgcccgta cgccaacgcc gtgccgccga ccttgacggc gggccgttcc ttgaccgccg
     8821 acacaggctc gataaatccg cccatcgaac ggacaagctg ataacgcgca acgccctgca
     8881 ccgtgtttcc gacaggctgg tcggttacgg cgttgtcggc cgggtcttcg taaaggaaac
     8941 tttcaaagct gcctttgcgc gcgttgaaga atcccgccag tttttccaac tcgtttacgg
     9001 acgcttttgt ccgcaatacc tcgaacgaca gcgaaaaccg ccattgcggg taggtgtagt
     9061 aggcggttcg gaactcacgg ccgcttgccg atttctgcgt ccccgtactc cataccgccg
     9121 ttttcttccg cccccacttc aagccgggga actcggggaa aaccgcattg cccatcagat
     9181 gattcctttc gctttgagca gggcgtcaaa ttcgtcttcg gacagttcgc cgccgccaag
     9241 cataccgacg gcttcggctt cgtccgcttc gctttgcaca ctgcccgacg acggcttgac
     9301 gcccatatac gacgctgcca agatatgcac gggcggatgt tcgcgccaat actcgttcag
     9361 gtgtttgata cgcggcaaat ccaagttgtc ggcgacgtag tcccacgtcc agccggtaga
     9421 ggcgcaaacg tgggcaatca ccgcgccgaa acttaatccg ccgccctggc ctcccccgct
     9481 tgtgcggctt cctgctcctt gcgtttcaag cccgaaacgt ccatcacggc ggcaaacact
     9541 tcgttcatgt tgccgatatc gatcaaatcg gccgcttctt cgcgcgttaa atccgggtaa
     9601 ttgcgcttca gggcggcgtg ggcgcaatcg ataacggtag agatttgtcc ggcatccgcc
     9661 gcattgccgt caaatgagcc gatgcgctcc tgcaactgct ccaacgcgcc cagcgcaata
     9721 ggcggaatca cataatccgc gccgttcagt tctacggtta cgcctttaat ccgtacggtc
     9781 atttttattt ccttgacttt ggttgaataa aaaaagccgc cttttcagac ggcatataaa
     9841 cattactcct gaatccacag cgtaccgatt ttaaagcccg cctcgtcggt tgaggcggta
     9901 aagtcgattt cggggacgga gaagtcgtcg tttttagtcg agaacaagcc caatttgccg
     9961 ctggttacgc tttccagttc caacagggct tttttgcctt tgaactgcgt caggtacctc
    10021 atcttaaacg tcggcgtgtt gcccgtcgcc atattggtca gctcgatttt cttcgccggg
    10081 ggcatcgttt gcgtataggt aaagctcgga taaacggttt tgcctttatc cgagctgtta
    10141 aaggcgtaaa cgcccgattc cgaaaccgta tattggcctt gtttcgggga ggatgctact
    10201 ttgatataag ccgtgccgtc ctggcccatc acgcccgcgt cctcagcgaa cttgccgccg
    10261 ccgggcgcgg ctgcctgaag gtaagaataa ttgtcgccat cgaggacttt gcccgtagtt
    10321 tccgcccaaa gcgccttcat cgtgccggtt gcatattccg taccgaaaaa cagggtattg
    10381 agggtcaggc cgttaatcaa cgcgcctttc attttgcccg acaccttgac cttgccttgc
    10441 gccacagcca aaggataacg gttttgaccg tagaactctt tcaattccgc cgacaaatcg
    10501 acggacattt cctgcaagcc cataatccgc acgggcgttg cgttctgtac acggttgccg
    10561 taagcatccg taatcatttg ggcgaacact tcgccgctgc caaacgtcaa ctgcatgaca
    10621 tccctttcaa aaaaagccgc attacgcggc gcaaatcaca atcggaataa tacaaaccgc
    10681 ctgttcgcca agcgtcccct cgtcggtttc caccgtaccc tcgacgcggc aatactcgat
    10741 atccgcgccg tccgccgtta aatccgtctt gcccgtaacg gggtggacgg cgttcacggc
    10801 attgcacacc gcgtcaatca gcggattcat gacgggcgcg ggcggttggc ccgccgtctg
    10861 gacgtacagg taaacatcgg cgcgcaaaat ccacttggtc tcccgccccg tcaatgtcaa
    10921 cgcctgcata tcgccctgcg ccataaataa cgcgggctga tcgtagcgtt tcacatcgtt
    10981 ccagtgcacc agcttccggc tcttggttac gaagccgtcc aatgcgtcca gcttcgccca
    11041 cagcgcggaa taaaccgcct cacgattcat ttcaacacct ttccaatcga tttttgcagg
    11101 tcgtccgcga acttcggcgt caaatcgcgc aatgccgaac ggaggaacga ccgctccggc
    11161 aacttcacat cacgggtatg ggcgcggacg cggacatatc gcggcggttt cagcggcttg
    11221 ccgaacgcct gcctgacctg gcgcagcgaa gccttaacgt tgacactgcc cgcgaaacca
    11281 tactcatgcg cgatgccgta acggacgttc gtatcgactt cgcccgaaac gacattgccg
    11341 gaaacgttca cgcgctggtg tacggagcgt cgcaggttcc ccgtccttac cctcagcacc
    11401 cggcccgata accggttctg cttgacttcg cgctgcaggc gcaatgccga cctgcccacc
    11461 gatttgacga ccgcatcttg aacatcggcg gcatatgctt taaaaaccgc cgccagaata
    11521 ccgccgccga taaattccat cttcagcatc acacgccctt tcgtttgtac tcattgagta
    11581 tcgcaaacgc cgacggcggc ataccgccgg aattaccgaa cgtagaaaaa gcgacggttt
    11641 cgcctgcaag cgtcttgctc cgtacgccct tgccctcgat ttcgttcacg cgctgcaccg
    11701 cgataatcat caccgcttcg cgtatgtcgg cgggcatgcg ctcataaccg gcgcggtacg
    11761 atacctcgac atttttcagc ccccgcgcga aaccttcggg gcgcatcagc agccagttgt
    11821 caaactccca gccgccggca tccgcgccgt tgattttgac ggacgacacc gacaggacgg
    11881 gataattgtc caacacgatg cggtctttgc cgttgccgtc gtaccgcccg acataatcgg
    11941 cggccaggag gctgcgcccg atataggctt ccgccgccgc cgacacgccg tcaatgacgc
    12001 tgaggaagaa atcgtcccgc cggtcatgtt caacgccgat acgctgcttg aactcctcaa
    12061 gcgataccgg ggcggccatc gttattcagc cttttcggct tcggcaggtt cggcttctgc
    12121 ttcggcaggt tcggcagctt cggcaggttc ggattgtcct gccggctgcc cggttttagg
    12181 tttacgcccg cgcttggctt tttcaggccc ttcggcaggc tcggaggcaa cgttgccaaa
    12241 accgaactga tacaggaatt ccgccgcctc cgacggcact tccacgatac cgttttcatc
    12301 tgccgcgtag ctttggctgc cgaaggaaac gtcggtaaac ccttcgggcg cttttaattt
    12361 gactgtcatc tcaaaatctc caaaagaaaa agccgtctga ttttcagacg gcatatcggc
    12421 ttaacccacg ttggtaatca taccgaaggc aggcatgaac ataccttgca gcacctcgtc
    12481 cgcatagacg ccgtattcgt acatacgggt gcgcagcggc cattcgattt ggtaatactc
    12541 ttggcgcgta cgcacttgca gcagattgcc gacgccctga acgtaggcgg gcaggcggct
    12601 tgagtagaac aggtaagtac cggcaggcag gttcgggtgt accacgatgt tcaggtcgtc
    12661 gcccgtgatc ttgttcatat acgaaccgac aacgacaccg gcgcggatgt tcgccgtatt
    12721 gttcacgtca acgttcaact taatcaacgg cgcgccgctg tttccgataa tcagcttggt
    12781 cagcgccgcc aaatcgcggg cgttgacgta gacggtatcg ggggacaggc ggtatcgggt
    12841 aaagaaatgc gcgaacgcct cttcaaattc atacacgccg cccgcgttgt cggaagtcag
    12901 gccgccgcct ttgttgtccg accagaacgc gcccgaatca ggcagggcga tttgggtcaa
    12961 caggccgtca aattccaaaa tagaagtgga attgtcttcg gacggcaggg aagcggcggt
    13021 ctgggtacct tcggcatcag ccgaaatttc cactttggcg gcagtggtaa ccgcgcccag
    13081 tttttcagaa ccggccgcac cccagtacca agcataggca accgcgccgc gaacagccgg
    13141 aatcacggcg gttacttttt tgcctgcccc aataccggaa acggaagccg ccgcagattt
    13201 tcgggcggaa ccgccgccga acgtatcggt tgtaccatcc gcgtttcggc gtgtgatttt
    13261 ggcagggact tgggcagttt taatgttcgg gctttgtccg gttgcaccgt tgttcgcgcc
    13321 tgccacatcc cagtatgcct gcaagcccaa agccacacag attacagaca aggtgctgac
    13381 gccgagtttg cccgccgcgt cggcggaaac gacggcggtc ggtgtaggtg taacgcctgc
    13441 tttcaggctg gtgttgccgc ccagcaaaat catttcttcg gcaaccatag tagcctgaag
    13501 ggtttgggcg accgccaacg ctttcacgtc ctcgaaaccg cgcgcggcgt aatccgcttc
    13561 aaaggttact tggttttcca agccgatggc gcggaattgc gcgttgcgtt ccaccgtttc
    13621 gtgattgatg acgccgccgc gcctgccttc gccgataccg gcgcgttggt tgccgacgtt
    13681 gatattcgtg acggctttcc agttcgagcc gatggtgcgg ccgccgccca cgcgcgggat
    13741 acggttgcgc aacggggtca ataccgggta gagtttttga gacggcgcgg aaaggtcata
    13801 ggtttgcagg ccggcggtaa agccggccgg ctgggtaaaa cctttgtcag gcggcccgcc
    13861 gcccgcttgt gccgacttca tcagttcaat cgtttcttgt gtgagttggt tcacgttcat
    13921 ttatcgctcc tgataataaa aaaaccgcct gtaagcggtg ttacagacgg cctgtttgtt
    13981 ttgccttaat gagtgtcgcc acgtcatcca gcgtgccgtc attctttaca atcggctgaa
    14041 aaccttttaa cgggtcttcg ccgttatctt ctgccttact gatagctttc gtactgcctt
    14101 tcggcggtac tgcctgtttc ttcaggcttt cgatttccgc ctgtgctttg gcaagggcgt
    14161 cattcgattt tttcagcgcg tcttgtgcct ttgccagttc gtcagccgat tccgctttgg
    14221 caagatcgcc tgatttgccg gctttggccg ccaaaccgtc ggccggctta tcggcttctc
    14281 ttgccgccaa cgctttcagc gattcggcaa ggccggccgc cgattctttg atttgcgcgg
    14341 taacggcttc atcgatgttg tcgtaggatg cgtcctcaac cagccatttc agcgacatca
    14401 atacatcagc cggtgatttg acttggtaca ttgatttggc ggccgtcccg cctttcggct
    14461 tgtcggcttt agccaatacc gctttcaagg cggcgatttc agattcggac aattcgacgc
    14521 ttgccgattt tccggcttcg tcctttttgc cggcgtcctt atcgccttta tcttcggttt
    14581 taggcccttt atcgccgtct ttaggatttt catcttcctc ttcggttggt ttgtcagacg
    14641 gcttgccgtc tttatccgcc gcccctgcct catctttcgg tttgtccgcc ttaaagcagg
    14701 taaacaccgc gtcgggattg gcagggcggt cgacaaggct gatttccgtc agcttcaagc
    14761 ccgtgatttg cgacttgttc aaatcatcgc gggcggtaac cctgccgccg atggaaaagc
    14821 ctttgtaaac gcctgtcttg actttcgtca ccgcaacagg gtcaacgata tgcgccccga
    14881 aaaatgtgcg cccgtcgtct tccacgttga tttcaatcgc cgttcccgcc gcgtttgagc
    14941 cgtgcatctc gcgcaccgcg ccgaacttca tataatcggg aatcgccgcc ttcattgctt
    15001 ccgccgcgac gacttcgccg tccgaatcga ccgcttcgct cgaggcatag ccccaaactt
    15061 tgaccgtgcc gtcgtcctgc gcctccgtct tggcgatttg cgcgtataac ttcgtcattg
    15121 gtttgctcca aaaaaaagcc gccccgctaa ggggcggcaa aacacacccg cctgacccga
    15181 aggaatcaag attcaggcat atcccctgcc aaaacaggca caaccgcgca ccggcagttc
    15241 ggatgaccgg gtatcgtcaa cgcaccgtgg gagaaaggct cgtgcaaacc gattacgccc
    15301 atcccgccgt tggcattgca gacatcggac accttgtcgt cttttgcggt tatccactgc
    15361 ttgccggcaa ccagccccgt ttcttcccag cctatcaggt tgcccatacc gtccgccatc
    15421 gccgtttccg ttcgggcaat ggttcgggcg cgtgcgttgc cgaaagcatg ggattctttc
    15481 aggcggcctg ccaattcctg cacactgtcg ccgtttcgca tggcttcggc tacttgggcg
    15541 cgtatcattt cgcgcgtccc ttcggtgatt tgccactcgg cggcaggatt tcggacaagc
    15601 ccgccgcccg cccgcttcat gccgaccatt tcggcggcgc gttcatgcgc ccacttgacg
    15661 gcgcggctgc gaatgttcgt aaccataccg gcggcaggtt caggcattac gcgcaacaag
    15721 gcggcaaccg ccccgtcttc cgcaacacgc ctgattatcg gctcgaccac atcggacaat
    15781 cccgaccaat cgccaaaatc caagccgttt gcaacaaccc ctgccgcgcg gctcaattcc
    15841 gccgccaaat ccccggcctg ccaatcgacg gccgcaccct caatcagcgc cgcgatttgt
    15901 tcggccaagc cgtcaatgcg tgtcagcaaa taagcctcaa taagcgcggc agattcgtct
    15961 tcgctcatcg ggctttccga ctttcccagc ttttcagcct cttggttcgg cggctcttcg
    16021 ggctttcggc cgtcttgctt atccggttca ggctgcccct gccccggtaa cggctccttg
    16081 cccagttcgg cgcggatttc gtcggcggtc aagatgcctg cgtttttgta gatggcgtag
    16141 atttcagcct gttctttcgg gttgagcgat tcctccccct tccagacaaa ctcatacgcc
    16201 gccatatcca tgtaacgggc aagcacgtcg tcaatcaggg cttttaccca gtttttcagg
    16261 ctgcccatgc cgtctgaaag cgactgctcg cggctcgtct ctgccacgct gcggtttacc
    16321 tgcgccacga acggcgtagg ctcgacacta aacgcaaagc agacgacacg cgccagccat
    16381 tcgtcgtaaa cgtccttcaa cggcggctgc ttcgtctcgc ggaagtttcg ggacaactcg
    16441 cccggcacga aacgcatttt gcgccgctgc gccgtttcgc ccgacagcag caaatcccag
    16501 tattcttgaa accgcctgat gtcgtccgcc gaccacgttt caggcacgcc gactaaagca
    16561 tcgggcacgc tgcccgccgt gtagtattcc agcgcgtgaa cctgccgttt tagggcgata
    16621 ttcacggtca tgatgatttg ctcgacgggc gaatagccgt aaaccttgta acttcggtta
    16681 ttccgcgaac ggtaaatcaa ctcgtcagcc gtgtaatcga ccgccgccat gccgtgcagg
    16741 atttgctgat acgccgtatc gggcggtaac ggcatacgcc ccgtattgtc caaaacgcgc
    16801 ttaatcgtcg ccccgtccat cacttcgagg gcgtacaagc cgccgcccag tgttttgcgc
    16861 gggtagatgc acggcgcgtc aataacaaac aggtcttcca gcaagatgcg cagccagtcc
    16921 gcccacgtat gttctttatc gggcgaccgg aagaacgcaa cggcttcatc gacctttcgg
    16981 tctttccgtt gcgattcgtc gtcttcggtt gattcgacgt cgcgcttttg gattgtccac
    17041 ttaaggcact ccatttggtc tttacgcgcc tcgatcacca aacgcaatac atcgtagttg
    17101 tcggcaaggg cgcgtaattg cgcaaagcct accgcttcac gttcgcgcgg cttggaatgc
    17161 ccgacgttgt agaacggctc gtaatcaaac cgccgcccct ctgcctgctg cgcgacaggg
    17221 gctaaaggct cgcccgcgtc aaaccacccg tccgcattgc cggtaaaggc gtaacggaca
    17281 ccggcagcca cacgggcaat aaaaccttgt gataaaggtg tctttttact catttcatag
    17341 cctcaacctg cgattgcagg aaatcaatca tgcccgttcg ggtatccagt agctcgccaa
    17401 acgcacggct caaacagtcg atttggtcgt catgctgacc gtttgggaac atccgcattt
    17461 ccgcaatcag cgcgtctgtg ccccatgtgc cgtcatccaa caccatcaca ttgccgatgt
    17521 tgacctgtgc cgcgaacggt tcggcgcgtg taaccttgtc gcccgattca ggactggcag
    17581 atacagaaaa acccgccaac tggcgggtta gatacagggt ttgcgattta ccagcctgcc
    17641 cggggtcttg tgggatggat attttcgttt tcacgccgtc tttttgcgcc gtgttgcgca
    17701 atatcctgtc cctttcatcc gcgccgtact gaccgcgcac gatattggca atgatgtacc
    17761 gaccgtcttc tgtaacgcca agcctgccgc ctgccgtgta gtcgccgtcg ttcgcagtgg
    17821 atgccaagtc ccacgcgcga acccatctga tatttccagc gggcagggct ttcacaaatt
    17881 gcaggttgtc aggcttgaac gtaccgccgt caggcggcgc aggtttttgc aaatactgcc
    17941 cggcaaacac atacggcgcg gcttgttcca tacggtgcaa tgtttcaata tcatgctttt
    18001 caggccacaa cgccgtgccg tcgtcttgaa tagctggcag gcaaaggtgt tcccactctt
    18061 cgccgttgcc gccgtcaagc agccagcccg ccaagtcttt ctcgtgcagg cgttgcataa
    18121 tcagaataat aggcgtgtca gggctattct tccgagattc gaccgtgttt tgaaaccagt
    18181 caatgacgtt ctgccgcctg acctcgcttc gcgcttcatc agctttgtgc ggatcgtcaa
    18241 tgatgatgca gccgccgaat ccctcccgat gcttgcctgc accgaaacct gtaatcgtac
    18301 cgccagcacc tgttgcgtac atcacaccgc ctgcggttgt tttccagtga tggccgcttt
    18361 cgcctgccag tgccaaatca ggaaaaatcg cccgatactc ttcgtgttgc accaagttcc
    18421 gaatctgtac ggagttattg accgccagcg ccgccgaata gctcgcgtgg ataaactcgc
    18481 aatcaggcac gcgccccatc gcccacgcga taaagttcac gaccgcgatt tccgttttcg
    18541 agtatcgcgg cggaatattg ataatcaggc gtttcgtttc gccgttgaaa acacgctcga
    18601 gcgcgttgca gattggcgca tggtgattcg cccgctgcca aacatagccg cgcctttgat
    18661 aaaacatccg gcgcgtgaac atatacaggc ttgcagaact taaactgcga attactgatg
    18721 tttcaacatc gtcgaattgc cctagtgcca tttcattcta acttcctttg gagaaacggc
    18781 catacagccc ctcttatgat tagcgtttta tgctaaaccc tacgcaacac atcttccgct
    18841 atcttgcgat attcctcagc atcaagccgt acagccgccc tcatgctccc atcgcccgat
    18901 ttaacgtcaa gttccgtctt atcgccgtat ttcttcggcg caatcttgga agccgcccac
    18961 tttcgggcat ctatctgcaa tttcgccttt gaaaccgccg cgctctctgc ttgtgcacta
    19021 tcggcaatct cgatgatttc ttcagcgaaa tagtccgcct gcttttctct cgcgcgcgcg
    19081 tattggtggc gaaattcttc attagcttcc aaccaacggt aaatagtttt cattggcggc
    19141 acaccatctt cggcgcatat tgaacgtagg cttctgccat ttgcgatttt ttcgcatatc
    19201 ttatcggcca tgtctttcgt gtaatctgtc ggacggccta atttgcgttt cgtgccgctc
    19261 ataaaccctc ctcaaaaaag aaaccgtcta actctgaccc ctctcagaat tagacggcaa
    19321 acacacactc gccacatagg aaaaacggaa cgccgctacc tgtacaggca gaagctcaaa
    19381 ttcggacagc ctaaaacgca aaaacccgca cattgttatg tacgggctta aaaattcata
    19441 tccttcgggc gtgcgaaaat ccccgcaagg gtaacgattt gaattataca cctattgccg
    19501 gaaaaaacaa caggccgtct gaagattcag acggcatttg tattccctac tgcatcaaac
    19561 cgccgacagg ttgcggattt cgggcagtat cgggcggatt ttttctgcgt gttccgcgtc
    19621 ggcgtgtgcg cttaaggctt cgagggcgtt tgcggcggct ttgaggcggc tgcgcgtttc
    19681 cgcccagacc gtccacatcg taaccgcctg cctgcagccg agctgcttca gcggcaggga
    19741 aacgtctctg cccatttgga tggcccgcgc gccgtagctg acggcgacgg cgaggtcgta
    19801 cagggcgttg ccgcttatgg gcagggcggg ttgcggcgcg ggtaacggtt cgcggtcggg
    19861 gacttcgccg gccaaacccg tgtgcagtgt caatgcgtgg acgtaggcga cggcttcggg
    19921 cagcttcccg gcggggatgt cttcgatggc ttcgacgttg aagcgttggt gtaccatact
    19981 gtacgctgag gagtagtcta tgcctttgcg tccgacaagt gcggcaacgg cttggcgcaa
    20041 tccggtacgg tcgtcggcgg tggttttttg tccgacttgg tagccgcctg ttttgcggat
    20101 ggtgggcagg acttcggatg ttacccattt gcggaatttc caagcggttg agccttgttc
    20161 cattgcttta cggctgcgaa gaatcaaaat atataaaccg ctttcgttga tgatgttcac
    20221 gttaccgccg cctccgtgaa tttcagaccg ccctatgtta aacatagacc gctcgtcatc
    20281 atctaatttt tcaagtgctt gcgttgggtt ctggatttct aaagccttac atacatcggc
    20341 ggcaacaaac caagtcaagc cgtctttttc aaaggcacga acgggagaag tagtattgaa
    20401 attaaatgat tgaattacgt tcataatgaa gtttcctagt gagttttctt aatgcccgtt
    20461 agggcggacg cgtggttaag aaccctcact agatgggcgg acttattccc cttacgggta
    20521 ttgtattcgt cgcccacgcg tccataagaa acttcggttg tgccatcgaa acaaacaaca
    20581 cgaaaggaaa cttacagata tgaaaaaatc acattgacgg agtgattgcc gctagtgtgt
    20641 ggttcttacg ccacgaacag gaatataaaa caaacccccc tgcacatgca aggggttttt
    20701 tactatttct gttcaatcga aacttccact ttattgtctg tgataacgca atatgcccga
    20761 taatcaatct gcattccaag cccgttttta gccgtaaatc cacgggtaac caagacattg
    20821 ccattctgat ttactccggt tgccgtgtct aggatgtgcg ggtcaaacgt tctgggatga
    20881 ttcaattgag ctttgattgc ctcatcacac tgactgatga cggctgaact atcaatcttt
    20941 ttatcctgtt cagcggttga tttccgaccg gaattaaggt cgtcagtaga tacaaaaaag
    21001 cgtttgccat tcctgcaatc tacaaagaaa accatttgtt ttgctttcgg gttgctgcga
    21061 gaatctgaaa tatcaacagt ttctactgca tcgcatgaat cctcccgagc aatatgttcg
    21121 gctatccgtg gcaaataacc attcatttca ttgacagcct tctcgcccca ctgcttgtac
    21181 aacttcggat aagctttctt atcgtactta aattcccgta cgcctgaagc aattttccca
    21241 tcaatcttct gcttttcggc tgccgccttc tcttctggcg ttaattcctt tttcggcaca
    21301 gcgcacatag cgacaaacag caaaacaaaa ataccgccca caatcagctt gattttcttt
    21361 gctttttttg ccttagccgc agaattaacc tgcctttggt tgtattgctg ccgccgctct
    21421 tctgggtttt gtggttcatt ataaagggcg accttcaccg cttgcaggaa tgcgtcgttc
    21481 ttctccacca gcatgcggcg gccatcaaaa aacacaacct ctatcacgcc gccgtcctgc
    21541 acctcataac ctgcaatatc aagcagcgca accttcccac cacgagaaag aacaatacaa
    21601 tcttttttta agacggcttt ccctttatga aaatcgccag ccagtatgtt gatatatgcc
    21661 atttccccat ctcccattaa gaagtgttaa tttccacaat cataccgtaa ccgaaaagaa
    21721 aatcaaacct tctcaaacag caaatcaaaa tcgtcccctg ccgcctgccg tatcgccccg
    21781 taccacgcgg ccaagcccaa atccgtctgc aaatgcaggg gcccctcgcc gcgccgcctg
    21841 atttcagcct gcaaacgttc ctcgtaggct gcctgcgact tcgcaccgat gccgaacgaa
    21901 atacggacgg cctcttgttg cggcgcatcc accttcgccc atgcctgcag agtcagaaac
    21961 atggcatctt cgccgtatct caagccgatt tcaggcttat gcgggcaagc ttcctccccc
    22021 atataacggc cttcgatact caaacatcta tttaggctgc gcgtatcacg gtaacgacgc
    22081 tcaaaagcac gcgccaagtc gttcataaat tcaaattctt gttgattcat agcttgatta
    22141 accctttttc atgcaacaaa accaaagtcc gcattacacc ttccgcgaag gccgtctgaa
    22201 gttccccttc cgcacaatcc gccaagcggt tctatacatc aaaactccca aatgattcca
    22261 aa
//
LOCUS       DAWTCI010000024        22120 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWTCI010000024 DAWTCI010000000
VERSION     DAWTCI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22120
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.024"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            96..917
                     /locus_tag="WM481_000958"
     CDS             96..917
                     /locus_tag="WM481_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HFA9080300.1"
                     /translation="MSSPRQVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATVSGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYKDKLFGLTIQPERLQAIRQER
                     RPNSAYARIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(1003..1665)
                     /locus_tag="WM481_000959"
     CDS             complement(1003..1665)
                     /locus_tag="WM481_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9080301.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTQLARHGLPEKSMDEIRTQ
                     ASHGAAGLLKLGANITPEHPDYTAWRTEYLEEYDSRYAQDTTLFDGVNELIAELGRRG
                     IKWGIITNKPMRFTDKLVPKLGFAVPPATVVSGDTCGEPKPSIKPMLHACGKIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(1703..2530)
                     /locus_tag="WM481_000960"
     CDS             complement(1703..2530)
                     /locus_tag="WM481_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFA9080302.1"
                     /translation="MNPDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDTPVLVAPSQLTPRKMNTAEGYAAMLHAITHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLVRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVEQRGDSATCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKHAV"
     gene            2913..3536
                     /gene="lolA"
                     /locus_tag="WM481_000961"
     CDS             2913..3536
                     /gene="lolA"
                     /locus_tag="WM481_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFA9080303.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTLPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVRATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            3809..4944
                     /locus_tag="WM481_000962"
                     /pseudo
     CDS             3809..4944
                     /locus_tag="WM481_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
     gene            5039..6071
                     /gene="pglE"
                     /locus_tag="WM481_000963"
                     /pseudo
     CDS             5039..6071
                     /gene="pglE"
                     /locus_tag="WM481_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(galactosaminyl)2-N,
                     N'-diacetylbacillosaminyl-alpha1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglE"
     gene            6112..6642
                     /locus_tag="WM481_000964"
     CDS             6112..6642
                     /locus_tag="WM481_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFA9080304.1"
                     /translation="MNAIRTFQNRMPEIHETCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDDVMIGAGSLVPPRKRLEGGYLYIGSPVRQVRLLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(6810..8405)
                     /locus_tag="WM481_000965"
     CDS             complement(6810..8405)
                     /locus_tag="WM481_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFA9080305.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFGIIKGINNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPA
                     PKPRDATMRMVGPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHNRELAEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(8512..8907)
                     /gene="hisI"
                     /locus_tag="WM481_000966"
     CDS             complement(8512..8907)
                     /gene="hisI"
                     /locus_tag="WM481_000966"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFA9080306.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDTIVMLIAQNGGIACHTGRES
                     CFYKKWNGGAWETADAVLKDEKEIYGSTH"
     gene            complement(8938..9705)
                     /gene="hisF"
                     /locus_tag="WM481_000967"
     CDS             complement(8938..9705)
                     /gene="hisF"
                     /locus_tag="WM481_000967"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFA9080307.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AVEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRTMREAGIEVRL"
     gene            complement(9718..10455)
                     /gene="hisA"
                     /locus_tag="WM481_000968"
     CDS             complement(9718..10455)
                     /gene="hisA"
                     /locus_tag="WM481_000968"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFA9080308.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPADTALHWFEQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGMRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDLVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQSVRIPVIASGGLTGLDDIPALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(10488..11126)
                     /gene="hisH"
                     /locus_tag="WM481_000969"
     CDS             complement(10488..11126)
                     /gene="hisH"
                     /locus_tag="WM481_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFA9080309.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNTKIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDPQGCRLKVPHMGWNTVRQTQNHPLFQGIPQNTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(11256..12758)
                     /gene="pta"
                     /locus_tag="WM481_000970"
     CDS             complement(11256..12758)
                     /gene="pta"
                     /locus_tag="WM481_000970"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFA9080310.1"
                     /translation="MAKVLVVPVSAGLNTSAAAQAFAKALDAQVFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NTDADALAHQLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQAGKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(12943..14001)
                     /gene="fbpC"
                     /locus_tag="WM481_000971"
     CDS             complement(12943..14001)
                     /gene="fbpC"
                     /locus_tag="WM481_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFA9080311.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIIGASGCGKT
                     TLLRCLAGFEQPDSGEISLSGKTIFSKNTNLPVRERRLGYLVQEGVLFPHLTVYRNIA
                     YGLGNGKGRTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCRLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPAASIHAVVLKTTPKARHTEISLRAGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(14022..15539)
                     /gene="fbpB"
                     /locus_tag="WM481_000972"
     CDS             complement(14022..15539)
                     /gene="fbpB"
                     /locus_tag="WM481_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFA9080312.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLTAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LILGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTADDVHTLSTAVWEYTSDAQYAAAAPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(15607..16602)
                     /gene="fbpA"
                     /locus_tag="WM481_000973"
     CDS             complement(15607..16602)
                     /gene="fbpA"
                     /locus_tag="WM481_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFA9080313.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYVPT
                     SGAFLEQIVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLAGKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(16993..17334)
                     /locus_tag="WM481_000974"
     CDS             complement(16993..17334)
                     /locus_tag="WM481_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFA9080314.1"
                     /translation="MTDTDTQADRFEQMMRQAVDKLFEQHDGKLESMDGREQELVLIW
                     RAEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDI"
     gene            complement(17392..18768)
                     /gene="argH"
                     /locus_tag="WM481_000975"
     CDS             complement(17392..18768)
                     /gene="argH"
                     /locus_tag="WM481_000975"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFA9080315.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDQRLAEWDIQGSLAHAQ
                     MLTRSGVLSENDLTDIRRGMSEILEEIRSGKIEWPLDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDFERMADCRKRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPGNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEALQGFSKLISDDVYGVLTPEGSLNARNHLGGTAPEQVRLQV
                     KRWREMSA"
     gene            complement(18787..19656)
                     /gene="galU"
                     /locus_tag="WM481_000976"
     CDS             complement(18787..19656)
                     /gene="galU"
                     /locus_tag="WM481_000976"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFA9080316.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKANPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETKLEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(19684..20283)
                     /gene="rdgB"
                     /locus_tag="WM481_000977"
     CDS             complement(19684..20283)
                     /gene="rdgB"
                     /locus_tag="WM481_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFA9080317.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFKPYGITVLPQSPFGIPECP
                     EPYPTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCSYVCVLVFVRHKDDPRPIIAEGIWHGQWHDTPLGQNGFGYD
                     PYFYLPEHGKTAAELDSEVKNRESHRAQALADLIRKLAL"
     gene            complement(20276..20509)
                     /locus_tag="WM481_000978"
     CDS             complement(20276..20509)
                     /locus_tag="WM481_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080318.1"
                     /translation="MNRRKIYLLSVALFTLAFMLPVLLGAYLLTAGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(20617..21150)
                     /locus_tag="WM481_000979"
     CDS             complement(20617..21150)
                     /locus_tag="WM481_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFA9080319.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RIEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGGAE
                     EAKKVIKESIERWNKQA"
     gene            complement(21252..21710)
                     /gene="nudB"
                     /locus_tag="WM481_000980"
     CDS             complement(21252..21710)
                     /gene="nudB"
                     /locus_tag="WM481_000980"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFA9080320.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLEDGQLQDRHDSTVYEIYHHWRHRYPKGVFENREHVFR
                     AEIPRDTPVVLQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            21921..21997
                     /locus_tag="WM481_000981"
     tRNA            21921..21997
                     /locus_tag="WM481_000981"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21955..21957,aa:Pro,seq:ggg)
BASE COUNT         4692 a         5472 c         6626 g         5330 t
ORIGIN      
        1 gggtgtaatc cgaggtaggg gcggctgggg tgcttctccc ttgtctgccg ctgctgttat
       61 gatgatattt ttattctgta tttaaggagg gggtgatgag cagtccgcgc caagtgtttt
      121 atatttccga ccgcaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt
      181 ttggcaatct gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc
      241 gcgcggtggt ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt
      301 ttgtcagtgt ggttgatgac gaaatccgcc ggattattaa aggggcggat gcttttcaga
      361 ttaatttctt tgagactttt ttgggactgt tggagaagga actcaatacc gaagcaacgg
      421 tatccgggca ggggcatcac agtatcggca atacgaagcg ttacgatgcg cgtatggagg
      481 cagtcaattt ttctttgaac catgatgacg gggtcagcga taagaacctt caggaggcgg
      541 atgtgattct catgggcgtg tcccgttcgg gcaaaacgcc gacctgcctg tatctggcgt
      601 tgcaatacgg tatccgtgcg gcaaactatc cgctgattcc cgacgatttg gaatcggccg
      661 atctgccgcg tatggtcaag ccgtataaag acaaactgtt cgggttgact atccagccgg
      721 aacgtttgca ggccatccgt caggaacgcc gcccgaattc ggcttatgcg cgcatcgaca
      781 cctgccgcag cgaagtggcg gacgcgcaga gtatgttcag acggcatggc attccgtttg
      841 cgaatacgac ggacaagtcg gttgaggaac tggcggtaca catccttcag gcgtgcaagc
      901 tcaaacgcag gttttgacgg gctttgattc ggtttgaagg tggaaatgcc gtctgaaatc
      961 aggtttcaga cggcagtttt atgtttgtgg ggcggatatt tttcagggct gtattttgtc
     1021 cagacattcg agcagatcga gcggcgtgcg gatgtggaaa tccgcctgcc atgagccggt
     1081 atcgtcttca tcggaaatat agccccattc ggcgaggacg gttttcatac cggcgttgcg
     1141 tccggcttgg atgtcgcgtt ccgcgtcgcc gacgtagagg gtgtgttgcg ggtcggcgtg
     1201 gatttttccg caggcgtgca gcatgggttt gatgctgggt ttgggttcgc cgcaggtgtc
     1261 gccgctgacg acggtggcgg gcggaacggc gaatccgagt ttgggaacga gtttgtcggt
     1321 gaagcgcatg ggtttgttgg tgatgatgcc ccatttgatg ccgcgtctgc cgagttcggc
     1381 gatgagttcg ttcacgccgt cgaagagagt ggtgtcttgg gcgtagcggc tgtcgtattc
     1441 ctccagatat tcggtgcgcc atgcggtata gtcggggtgt tcgggcgtga tgttcgcgcc
     1501 gagtttgagc aatcctgccg cgccgtggct ggcttgggtg cggatttcgt ccatgctttt
     1561 ttcgggcagt ccgtggcggg cgagttgggt gttgagtgcg ccgccgaggt ctagggcggt
     1621 gtcggcgagc gtgccgtcga ggtcgaacaa tacggcttgt atcatgtgtt ttcctttttt
     1681 ataaagtgcg ggacgaaagg tttcagacgg catgtttatt ttgtttcaaa ccctgctcga
     1741 aatcttccaa catatccaat tcgaagcggc tgaagcccgc tttttcccgc gcttcgatgt
     1801 tcacatagcc gcggaagata aacatatcgt aacgggcaat cagactgcgg aacagggcga
     1861 caggttccaa accgcgttcg cggcagaggt gttgatacca ccggttgccg atggcgacgt
     1921 gtcccacttc gtcgcggtaa atgatgtcca acacgccgca ggttgccgaa tcgccgcgct
     1981 gctccacctt cgcgcgtatc cccggcgtaa cgtccagtcc gcgcgcctcc aacacgcgcg
     2041 gtaccaacgc catacgcaac agcgggtcgt aggcggtttt gtacgccata tcccacaaat
     2101 ggttgtgcgc ctcgaaactg ccgtaatcga agccgaaagc gcgcaatctg tcgcgcacca
     2161 ggcggaagtg gtacacctcc tccttcgcca ctttcaccca gtcgcggaca aactgaaacg
     2221 gcagcgtgcg gaaacggtat gccgcgtcca aagccagatt gacggcgttg aactcgatat
     2281 gcgtaatcgc gtgcagcatc gccgcatagc cttctgccgt gttcattttg cgcggggtca
     2341 gttgcgaggg cgcgaccaaa accggcgtgt ccggccgtcc cgcgcggggg aagtccgccg
     2401 gcggcgcgtt tgcctccgcc ccgtccgcat tttgaacggc ggcaaacgcc tcatccgtca
     2461 gccgtccttt ttcatcgggg tcgcccgaaa gcagggcgcg ttccagcaaa gcataaatat
     2521 cggggttcat ctcaagtccg ccgtgttcgg aaaacgaata ttatagcgtt taaaaaaaac
     2581 aagatgaggc atataatctc cgcgattcgg cattccgcgc ccaaaccgtc aaatatagtg
     2641 gattaacaaa aaccagtacg gcgttgcccc gccttgccgt actatgttgt actgtctgcg
     2701 gcttcgtcgc cttgtcctga tttttgttaa tccactataa cgcggcatac aattgaaagg
     2761 gcagcgtggc gtgcctgctt tttccgagcg gtcaaaaaaa tcagccctcg gaaaacgcgg
     2821 tttgcaaaat gcaaaccgcc cgtaacgccg cccgtatgat tgttttgccg cgccgatact
     2881 ttgcgccaca cccatcccga caaggaaaaa taatgatgaa accgcacaac ctgttccaat
     2941 tcctcgccgt ttgctccctg accgtcgccg tcgcttccgc acaggcgggc gcggtggacg
     3001 cgctcaagca attcaacaac gatgccgacg gtatcagcgg cagcttcacc caaaccgtcc
     3061 aaagcaaaaa gaaaacccaa accgcgcacg gcacgttcaa aatcctgcgc ccgggcctct
     3121 tcaaatggga atacactttg ccctacaaac agactattgt cggcgacggt caaaccgttt
     3181 ggctctacga tgttgatttg gcacaagtga ccaagtcgtc ccaagaccag gccatcggcg
     3241 gcagccccgc cgccatcctg tcgaacaaaa ccgccctcga aagcagttac acgctgaaag
     3301 aggacggttc gtccaacggc atcgattatg tgcgggcaac gcccaaacgc aacaacgccg
     3361 gctaccaata catccgcatc ggcttcaaag gcggcaacct cgccgccatg cagcttaaag
     3421 acagcttcgg caaccaaacc tccatcagtt tcggcggttt gaataccaat ccccaactct
     3481 cgcgcggcgc gttcaagttt accccgccca aaggcgtgga cgtgttgagc aactgatgcc
     3541 ggccgccccg atgccgtctg aacgccgccg aggcttcaga cggcattttc acgcaggcgg
     3601 aacaatgtcc cgcattatcg gccgatcggg caacggaacg gcaaatccgt gaaaattaac
     3661 ggttgcgccc cggctgtttt tgccgtttaa tgcaaacctt gctgcaccaa gggccaagaa
     3721 agccgaccgg ccgcccccac agcttccgat gcaggcggcc cgtccgtccc tgcaatgttt
     3781 tttatttttg aacgaaaggt cgaaaaccat gaaaaaaaca ctggtggcgg caatcctgag
     3841 ccttgccttg actgcgtgcg gcggcggaag cgataccgcc gcccaaaccc cctccgccaa
     3901 gcccgaagcc gaacaatcgg gcaaactcaa catctacaac tggtcggatt atgtcgatcc
     3961 cgaaaccgtc gccgcctttg aaaaagaaac cggcattaag atgcgttccg actattacga
     4021 cagcaacgaa acactggagg caaaagtcct gaccggcaaa tccggctacg acctgaccgc
     4081 gccgtccatc gccaacgtcg gccggcaaat caaagcgggc gcgtatcaga aaatcgacaa
     4141 ggcgcaaatc ccccattacg gcaacatcga taaagatttg ctgaaaatga tggaagccgt
     4201 cgatccgggc aacgaatacg ccgtccccta tttctggggc atcaacacct tggcaatcaa
     4261 cacgcggcag gtgcaaaagg cattgggtac ggacaagctg cccgaaaacg agtgggattt
     4321 ggtgttcaaa cccgaataca ccgccaactc aaatcctgcg gcatcagcta tttcgacagc
     4381 gcaatcgaac agattccctt ggcgttgcac tatttgggca aagaccccaa cagtgaaaat
     4441 cccgaagaca tcaaagccgc cgtcgatatg atgaaagccg tccgaggcga cgtgaaacgc
     4501 ttcagctctt ccggctatat cgacgatatg gcggcgggca acctgtgtgc cgccatcggt
     4561 tacggcggcg atttgaacat tgccaaaacc cgtgccgaag aagccgcaaa cggcgtggaa
     4621 atcaaagtat tgaccccgaa aaccggcgta ggcgtgtggg tggattcctt tatgattccg
     4681 cgcgacgcgc aaaacgttgc caacgcccac cgctatatcg actacacgct ccggcccgag
     4741 gtggctgcga aaaacggcag cttcgttacc tacgcgcccg ccagccgtcc cgcgcgcgag
     4801 ctgatggatg aaaaatacac ctccgacgca tcgattttcc cgaccaaaga actgatggaa
     4861 aaaagtttca tcgtatcacc caaatccgca gaatccgtca aactgggcgt gaagctgtgg
     4921 caagggctca aagcgggcaa ataaccggaa tccctgccgt ctgaaacctt tcgggcggca
     4981 ggaaacggcg tgtccgcatt aagtcaggat taggattatt tagaaagatg agatggatat
     5041 gaatttaagt attgtcgttc ctatttataa tgtcgaaagt tatttggaag cgtgtttaaa
     5101 ttctatagaa cctatattaa gtaatgaaaa tgtcgaactt attcttgtga atgacgggtc
     5161 aaaagacgga agtgaagata tatgttataa atatatagat aaaatatcaa acaccaaaca
     5221 ccaaacacca aacaccaaac accaaacacc aaacaccaaa caccaaacac caaataccaa
     5281 ataccaaata tatatatcag gataaccaag gattgtcgga ggcgagaaat accggaataa
     5341 aaaattcaaa tggaaaatat atagcattta ttgattcgga tgattttatt aattgtcagg
     5401 ttttgctgga ttttcttggt aaagatgata gtgatatgcc ggatgtggtt tttttaaatg
     5461 cagttaaata tgataaggga agagtttcat attttggcga agattatcag cctgaaaaaa
     5521 tattaaatca atccaaagtc gaagttttga aaggattgtg ccgatttaga aaatttccgg
     5581 gttcggcgtg gaataagatt ataaaaagag aattgattat tagagaaaaa ctgttttttg
     5641 aaaagggaat ttattctgaa gatattgaat ggtcaatgag gttgtttaat gcggcaacaa
     5701 ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa gattctatta
     5761 cgggaactgt ttcggaaaaa ggtataaagt cattattata tattttggaa aaaaatgcga
     5821 aaatggaatt taatagggat atatcgagtt atctttattc ttttctttcc tacgaatatc
     5881 tcgttttgct ttttataatg acgagtaaaa atatagcgtg tgattctgat ataaaaagaa
     5941 gggcatatca tttaaggttt atgctgttaa agtccaataa gctgatatat aagctgatat
     6001 tcccgataat cacattattc ggggtcgata ttacaggcag gattttaaaa gcaatcaggg
     6061 ggaatattta ataaatcctt taacaatata taccttaccg aaggaggaaa aatgaacgca
     6121 atcagaactt tccaaaaccg catgcccgaa atccacgaaa cctgcatgat agacgaggcg
     6181 tgcgtcgtca tcggcgaagt ctcgcttgcc gaagatgttt ccgtgtggcc gtgcgccgtg
     6241 ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca ggacggcagc
     6301 gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct ggttatcggc
     6361 gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgaatcgg taaccgcgtc
     6421 ttggtcggta tgggcagcac cgttttggac gatgccgtta ttgaagatga cgtgatgatc
     6481 ggcgcgggca gcctcgttcc gccgcgcaaa cgtttggagg gcggctatct ttatatcggt
     6541 tcgcctgtga ggcaggttcg tctgctgacc gatgaggaaa aagccttttt gaaatattcc
     6601 gccgcacatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat cggcgttctt
     6661 gcgtcagtcc caaattcacg cggttgggat gcccgataac ggtatccgat gcgccttgat
     6721 tttgaccggc tgcgtttgag ttgcaggcaa aaatgccgtc tgaaagcctt tttttcggct
     6781 tcggacggca ttttattgcc gatttctttt tagagtttga ccgaatgttc gcgcgtttcg
     6841 tggaacacga tgtccggcca acgttcttgc gtcaacccca aattcacgcg gttgggggcg
     6901 aggtaggcga ggttgccgcc tgcgtcgatg gcgaggttgc ctgcgttggc tttttcaaat
     6961 tccgccagtt ttttcttgtc gtcgcacgat acccagcgcg ccgaccagat ggatgcgctg
     7021 tcgaacacgg cttccacgcc gtattcgttg gcgaggcgtg aggttacgac ttcaaactgc
     7081 aacacgccga ccgcacccaa aatcaaatcc gcgccgctca tcggtttgaa tacttgaacc
     7141 gcaccttctt cgccgagttg ttgcaaacct ttttgcagtt gtttgatttt cagcgggttt
     7201 ttgatgcgga cgctgcggaa cagttcgggt gcgaagaatg ggatgccggt aaacgccagt
     7261 tgttcgcctt cggagaagct gtcgccgatt tggatgttgc cgtggttcgg gatgccgatg
     7321 atgtcgccgg cgtaggcttc ttccgccagt tcgcggttgt gcgacatgaa ggttactacg
     7381 ctggaggcgg cgatttcgcg gttgatacgc aggtgtttca tcttcatgcc gcgctcgaat
     7441 ttaccggagc agacgcgcaa gaaggcgata cggtcgcggt gtttcgggtc catattggct
     7501 tggattttaa agataaatcc ggaaaatttc ggctcgtccg gcccgaccat gcgcatggtc
     7561 gcgtcgcgcg gtttcggtgc gggtgcccag tcaatcaatg aattgaggat ttcctgaatg
     7621 ccgaagttgt taatcgcaga gccgaagaac actggcgtga gttcgccggc gagaaattcg
     7681 tcgagattaa attcgttgga agccgcctgc accaattcga tttcgtcgcg caactgctgg
     7741 atttccaacg gaaagcgttg ttccaattcg ggattgttta tgcctttgat gatgccgaac
     7801 tcgtgcggca ggcgttcgcc gcccgcttca aagagataga tttcgtcgtt caggatgtgg
     7861 tacacgccct tgaagttttt gcccataccg atcggccagg taacgggcgc gcagcggatt
     7921 tgcaggatgt cttccacttc gtccaagagt tccaaagaat cgcgcacttc gcggtcgtat
     7981 ttgttcatga aggtaacaat cggcgtatcg cgcaggcggc agacgttcaa gagtttgatg
     8041 gtttgcgctt ccacgccttt tgccgcgtcg atgaccatca aggcgctgtc cactgcggtt
     8101 aaaacgcggt aggtgtcttc ggagaagtct tggtgtcccg gcgtgtccaa gaggttgacg
     8161 gtgtggtctt tgtagtcgaa ctgcatcacg cttgatgcca cggaaatgcc gcgctgcttc
     8221 tcgatgtcca tccagtcgga ggtggcgaat ttgccggttt tcttaccttt caccgtgcct
     8281 gcgctttgaa tcgcgcccga aaacagcagc agtttttcgg tcagcgtggt tttacccgca
     8341 tcggggtggg agatgatggc aaacgtgcgg cggcggcgca cttggtcgag gatttcttgg
     8401 gacatggttt tctttgcaaa aaggttcagg ccgcttttca gacggcccgg acagtgtttg
     8461 agacggcgaa attgtacaaa aaaatgcctg ataattcaat gttggaggcg gtcagtgcgt
     8521 gctgccgtaa atctcttttt cgtctttcag gacggcatcg gcggtttccc acgcaccgcc
     8581 gttccatttt ttgtaaaagc agctttcgcg cccggtgtgg caggcgatgc cgccgttttg
     8641 ggcgatgagc atcacaatgg tatcgccgtc gcagtcgagg cgcagttcgc ggactttttg
     8701 cgtgtgtccc gactcttcgc ccttcatcca ttgtttttgg cgcgaacggc tgtaatagtg
     8761 ggcaaagccg gtttcgacgg ttttttgcag ggcttcggcg ttcatccacg ccaccattaa
     8821 aacacgtttg gtttcggcat cttgggcgat ggcgcaaacc aaaccttttt cgtcaaattt
     8881 gacggcttca agcaggtttt tatccatatt tcctttcaga cggcatagtc ggggcggtca
     8941 gaggcgcact tcgatgccgg cttcgcgcat agtgcgtttg gcttcgcgga tggcgatttc
     9001 cccgaaatgg aaaatgccgg cggcaagtac ggcatcggct ttgccttcgg ttatgccttc
     9061 aatcaggtgc cggacattgc cgaccccgcc ggaggcgatg acggggatgt cgacggcttc
     9121 ggcaacggcg cgggtcagcg gcaggttgaa accctgtttc gtcccgtccc tgtccatacc
     9181 ggtgagcagg atttcgcccg cgccgcgttt ttgcatttcg accgcccatt ccaccgcatc
     9241 caaaccggtc ggattgcgcc cgccgtgggt aaagatttcc cagcgtgtgt tttcggggtt
     9301 gacggctttg gcatcgacgg cggcgacgat ggcttgcgaa ccgaaaaatc cggcggcttc
     9361 gttaattaaa tcgggacggg taacggcggc ggtgttgatg ctgactttgt ccgcgccggc
     9421 attgagcagg cggcggatgt cggcaacggt gcgtacgccg ccgccgacgg tcagcgggat
     9481 gaagacttgt ccggcaacct cttcgatgat gtgcaggatg gtgtcgcggt tgtcggatga
     9541 ggcggtgatg tcgaggaagg tcaattcgtc cgcgccttcg ccgttgtagc gtttggcggc
     9601 ttcgacgggg tcgcccgcgt cgcgcaaacc gatgaagttc acgcctttga cgacgcgccc
     9661 gtcttttacg tcgagacagg ggatgatgcg ttttgccagt gccataatcg gatgccttta
     9721 gtcgagggaa tctgccagtt gctgcgcttg ggcaaaatcg atgctgccct cgtaaatcgc
     9781 gcggccggta atcgcgcctg ctacgccatg tttttcggcg gcacacaggg cggggatgtc
     9841 gtccaagccg gtcagtccgc cggaggcgat gacgggaatg cggacggatt gggcgagttt
     9901 gaccgtcgcg tcgatgttca cgccgctcat cataccgtcg cgcccgatgt cggtgtagat
     9961 gatgctgttg acaccgtcgt cttcaaagcg ttttgccaaa tcaattacat gatgcccggt
    10021 tacggttgcc cagccgtcga tggcggccat accgtctttg gcatccagtc cgacgataat
    10081 cctgccgggg aaggctttgc acgcttcgcg caccaagtcg gggtttttga ctgccgccgt
    10141 gccgataatc acgtcgttca gtcccaaatc caaatattgt ccgatggttt tcaaatcgcg
    10201 catgccgccg ccgagctgca cggggatgtc tttggcgacg gcggcgagga tgtctttgat
    10261 ggcgggcagg ttttgcggaa cgccggcaaa tgcgccgttc aaatctacca ggtgcaggcg
    10321 gcgcgcgcct tgttcgaacc agtgcagcgc ggtgtcggcg ggcgaatcgg aaaagacggt
    10381 cgcctcttcc atcagccctt gtttcaagcg gacgcaacgt ccttctttca aatcgatggc
    10441 gggtatcagc agcatagttt ttctccttgt gcggggccgt gtcgggctta ccagtttaaa
    10501 aagtttttca acatcgtcag cccggcatcg tggctttttt cggtgtgaaa ttgcgtggcg
    10561 aatacgttgt ctttgccgac gatgcaggca aacggggacg ggtagtcgct ttcgcccaat
    10621 atggtttcgg gattttcggg ggcgaaatag tagctgtgga cgaaataaaa acgcgtgttt
    10681 tgggggatgc cttgaaaaag cgggtggttt tgggtttggc gcacggtgtt ccagcccata
    10741 tgcgggactt tcagacggca tccctgcggg tcgcggaggt cgcgggcaaa gcgtctgact
    10801 ttgccgccga accagcccaa gccgtcggtg tttccttctt cgctgtggtc gaataaaagt
    10861 tgcgcaccga cgcagattcc gaaaaacggt ttgtttttta aggcatcttt gactgcctcg
    10921 tccaagccgc cgcgcgtcag tgccgccata cagtcgggca tcgcgccctg cccgggaaaa
    10981 atgactttgt cggcgcggga cacgcggtcg gggtcgccgc ttaaaaagat tttggtattt
    11041 tttccggcaa gctgccccgc cgtccggacg gatttcaata cggaatgcag gttgcccata
    11101 ccgtaatcga taatggcggt ttgcatggct tcctcctctt ttttgcaata tggctgcgat
    11161 tttaacaaac aaatgtgccg ggctgataaa aatgccgtct gaaaacggga atctgtcttc
    11221 agacggcata gggttcaaac ccggaaagcc gtttgtcagc cttccatttg ttttgcctga
    11281 acggcagtca gggcgatggt gaacacgatg tcttccacca gcgcgccgcg agacaggtcg
    11341 ttgaccggtt tgcgcaggcc ttgcagcagc gggccgacgc tcaagacgtt ggcactgcgt
    11401 tggacggctt tataggtgca gttgccggtg ttcaggtcgg ggaagaccaa aacggttgcc
    11461 tgtcctgcca ccgggctgcc cggggctttg gatttgccta cgcccggcac ggttgccgcg
    11521 tcatattgca gcgggccgtc gatggcgagg tcggggcgtt tttcccgggc aagtttggtg
    11581 gcttcgatga cggtatcgac atcggggccg ctgccggagt tgacggtgga gtaggaaatc
    11641 atcgccactt tcgggtcgat gccgaaggct tttgcggaat cggcagactg gatggcgatg
    11701 tcggcaagct gttgcgcggt cgggttcgga ttaaccgcgc agtcgccgaa caccagaacc
    11761 tgatttggta gcagcataaa gaatacgctg gatacgaggc ttgcgcccgg tgcggtttta
    11821 atcagttgca aagcggggcg gatggtgttg gcggtggtgt gaaccgcacc ggataccaaa
    11881 ccgtccacat cattttgcgc catcatcatc gtaccgagta ccacggtgtc ttgcagttgc
    11941 ttgcgcgcgt cttcgggtgt caggcctttg gatttgcgca gttcgcacat cggctcgacg
    12001 tattgttcga ccaatgaggc gggatcgatg atttccaaag agtcgggcag gctgatgccg
    12061 cgttctttgg caacggcttc gacttcttcg cgtttggcaa gcaggacgca gcgggcaatg
    12121 cctttttcgt ggcagatggc ggcggcttgg acggtgcggg gttctgcgcc ttcaggcagg
    12181 acgatgcgtt tgccggcttg gcgggcgaag tcgatcaggt tgtagcggaa ttgcgccggc
    12241 gacaggcgtt ttgcttcgcg gcctgccaat acggatacgt ctttcagcgc gtcgctcgaa
    12301 ccgaagaagg tcaggccggt tttctcggct gccgcttcgg caacggaggc tgccgcaccg
    12361 tccacgacaa aaccttccaa tacgcccggc gcggcggcga agaactgttt ggcaaggttc
    12421 aattggtgtg ccagcgcgtc ggcatcggta ttgtcggaac ggacggcgaa gacggctgcc
    12481 gcgtcaaggg acaatgccag ttcgacgttt ttgcctgcga gatagatttt gtcggcatcg
    12541 ggcgcgatgc cttcgatgac gaggttggcg gcatcgagtg cggcaacttt gccgaccagt
    12601 gcgtcgaacc agtcgtcgct tttgccttgc gcgagcaggg tttcggcggt tgcgtcaacg
    12661 gcttggaaaa cttgtgcgtc cagtgctttt gcaaaggctt gtgcggcggc ggaggtgttc
    12721 agtccggcag atacgggtac gacgagtact tttgccataa tatatccttt cgtatgctgc
    12781 ggtgtgcggc atatgtggtt ggaaggggcg gcatatgggc agaaacggct gcctgcgtgc
    12841 cgtgcgtgcc gtgtttggct tggggcgcgc aggttgaata tagcaaacaa attctgtttc
    12901 caacaagata aatatccgca ggcttgtgga tgctgccgcc tttcagaggg tatttccggg
    12961 gaagaacagg gcgggaccgt ccaaatggag gacggcggaa atgccgtctg acagggtggg
    13021 ggcggaaggg aggttgagcg tgaggacggt ttgtccggct ctgaggctga tttcggtatg
    13081 ccgcgctttg ggcgtggttt tgagaaccac ggcgtgaatg gaggcggcgg gtgcggaatg
    13141 ggggtgaagg ctgaactgtt ccggacggat gagcagtgta ccgcgcgtgc ctgcgggtgc
    13201 gccgctttgg acgggcaggc ggcccaatct gcaatcggcg gtgccgtcgg cgttgagcgc
    13261 ggcggggaac acgatgcctt cgccgataaa cagggcggca tcaaggtcgg caggttgtcg
    13321 gtacaattcg tgagggcttg cggtttggag gatgcgcccc tgtttcatca cggcaatccg
    13381 gtcggcgtat tgcagggctt cttcgcggtc gtggctgaca aaaacggcgg attttccgtt
    13441 ggcgcgcagg gcggcaatca tgtcttcgcg aatctggcgg cgcaactgtt cgtccagcgc
    13501 gctgaagggt tcgtccaaca aaatcagttc ggggtcgggg gcgagggcgc gggcgagggc
    13561 gacgcgctgt tgttgtccgc ccgaaagttc gtgcggatag cgtccggcaa gttcggaaat
    13621 gccggtcaat tccaacatgg cttcgatgcg ctgtcgctct tgcgccgtcc tgcctttgcc
    13681 gttgccgaga ccgtaggcga tgttgcggta aacggtcagg tgggggaaca gcacgccttc
    13741 ctgtacgagg taacccaaac ggcgttcgcg gacgggaagg ttggtatttt tcgagaagat
    13801 ggttttgccg gaaagcgaaa tttcgccgga atcgggttgt tcgaaaccgg caaggcagcg
    13861 taaaagggtg gttttgccgc agccggacgc gccgatgata aagagaattt cgcccgggtc
    13921 gaggctgagc gaaatgtcgt ttaaaaccgg ggtgttttga aaacttttgg acaggtgtcc
    13981 gatgtgcagg gcggcggtca tagcggtgct tcctcaagct gttatttgaa ggcgtatttc
    14041 ttcagcagga atacgggtat gccggaaaat aataccagca tcagcgcgta aggggcggcg
    14101 gcggcgtatt gtgcgtccga tgtgtattcc caaacggcgg tggagagcgt gtggacatcg
    14161 tcggcggtca gcagcagggt ggcggtcaac tctttcatca gcttgaggaa gacgagtgcg
    14221 aatgcggcgg taatgccggg caggatggac ggcagtacca acgtcctgaa aataaagaag
    14281 tgtccgcgcc ccaatgttgc gccgacctgt tccatccctt tggggagttg ttccaaggaa
    14341 gtcctcaggg tggtttgcgc catcggcagg taaagcatga aataggcaag gatgacgacg
    14401 ataaaggttt ggtaaacggc aggggtgtag ttgatgctga aataaaccaa ggatagggcg
    14461 ataaccaaac cggggacggc gtgcagtaaa aacggcagcc tgtctatcca aacggttaaa
    14521 aaattgcgat agcgaaccga tgcccaaaca aggggcaagg cacataatat agtcaaaatc
    14581 gcacctaaag ccgatacgct taaggaacgg ataaaggcat caaatacgga tacgagcgcg
    14641 aatgtgccgg aagtgccgac catcatccaa tgtatcaata cgccaaaggg gataataatg
    14701 cccaaaatca acaagctgct taaaaaaaca atcgcgccga tctgaccggg cagtttgagg
    14761 gttttgacgg gataaggacg ggcaacgcct ttgccgctgt ggtaaatctt ggctttgccg
    14821 cgaaatatgc tttctccaaa tacgacgatg ccgcacaccg ccgtcaaaac agcggaaagc
    14881 agggcggcgg tattgttgtt gtaggacatt tcgtattctt ggaaaatggc agtggtaaaa
    14941 gtggggtagt tcaaaatgga taccgcgcca aattcgacca gcatatgcag ggcgatcagt
    15001 aacacgctgc tgccgatggc gggtttgagc tgtgggagga tggcggaaaa aaaggtttgc
    15061 aggcggcttt tgcccaagga caggctgact tcttcgtaag acaggctgat gcgtttgagt
    15121 gccgcctcga cgggcaggta ggcgagcgga aacgaggaca ggctcataat catcactgtc
    15181 ccccaaaagc cttcgacgcg gaaggtcagg ctgatccagg tgaaacagct gacaaatgcg
    15241 gggatgcaca aaggcagggt gattgccgtc tgaaaaaagg ttttgccgaa gaagcggtaa
    15301 cgttggaaca aaagggcgca ggcaatgccc aaaacaatgg aaatcagggt aacgcccgcc
    15361 atcatcgtca aggtgttgga gagcaaatcc cacatacgcg ggcggaacaa cagttcgacg
    15421 gcgcggttga tgccgacctg ccacgaacgc atagcgacat ataaaaaagg cagggtaagc
    15481 ggcagggcga tcagtaggat gaggccggta agccaaatgg gtattttttt aggagacata
    15541 gtgtttttca tcggcaaaac gggcggacag tataaatgtc cgcccgtttg acaatccgaa
    15601 aacggcttat ttcataccgg cttgctcaag cagccgggtg gcgtgttctt tttcggaaac
    15661 agtggtggcg gacacttggg gtgcttccaa cttggcgatg ggttccaaat tgaaggtgga
    15721 taccacgtgc ggattcaaag gatattcggc acggacggcg gtcagggcgc gctgtccttc
    15781 cttgccggcg aggaaggcga cgaatttttt cgcctcatcc ttgttttggg aggattttaa
    15841 cacggctgcg ccggaatagg taacgagtgc gccgggatct ctgtggcgga cgaaattcag
    15901 gcgggtgtgg acattttgta cgcctttttc acgcgcgaaa gcgtgccagt agtagttgtt
    15961 gatgagggcg gcatcgattt cgccgttttc aaccgcttga agggcgacgg agtttttagc
    16021 gtaaggcttg ccgtattctt tcaggccttt gagccatttc aatgcggccg cttcgccttt
    16081 cagtttgacg atggcgacaa tctgttccaa gaacgcgccg gaagtgggga cgtaaccgat
    16141 gcggtttttc catttcggcg tggcgtaatt caggacggat ttttccaaat ctttttcaga
    16201 cagtttgcgg gtgtcgtaaa cgacgacgcg cgaacgtccg ctcagtgcca cccagtcttt
    16261 tttggcggca accggcacgc ctttgccgcg tgtttcgttg atggtggagg cgggcagggg
    16321 ctctaggagg ttggctgcgg aaagggtggc gagtgccggg atttgttcgg aatagaatac
    16381 gtcggcgggg cttcggctgc cttcttcttt gatttggccg gcaagctggt cgcctttggc
    16441 actgttgagt ttgactttga tgccggtagc ccgggtaaag gcatctgcaa cggcttgtgc
    16501 cgcttctttg tgttggccgt tgtacacggt aatgtctgcc agcgcggggg tggcggcggt
    16561 cagggctgcg gcaagcagtg cgtatcggat agatgttttc atatcgattt tctcctaaat
    16621 gaatgagggt gtataccttg ttaagacata acggggtgta gtgtattcct tcttttttat
    16681 aaatgcaaat aattattttt taaatttgtt attatccgat ccggttattg tttgttctga
    16741 cttgtatttt ttccgtgcat cgcgcccgta aggcggaagc ggcgggcaat gcgtggcgga
    16801 atgtgggtaa aggcggcatt ttgatttgtc ggaatgctgg agaacctctc cctttaaaac
    16861 gccgtctgaa caaggttgcc ggaatagtat tgccatcccg gcagatacag tttgtcggga
    16921 tcttgccaat attgttcatc cagactgttc ggcagcgagg cggttttgtt gtcgagatgt
    16981 tttgttaatc cactatatat cccacatttc ttttaggttt ttaccttccg attggaggtg
    17041 gcggatttct tttcccaatg cgtctgccgc ccgatggatc agcattgcgc tgtgtaccgc
    17101 gccgattttt ttcagaacgg aacaagtctt ttcggcggcg ggaaaacccc agttgcagac
    17161 aaattgcagt atgccgccgt tgccgatgtc ggcttccgcc cgccaaatca aaaccagctc
    17221 ctgttcccgt ccgtccatgc tttcgagttt gccgtcatgc tgttcaaaaa gtttgtccac
    17281 cgcctgccgc atcatctgct cgaagcggtc ggcttgggta tccgtatcgg tcataatatt
    17341 cttgcctttt aaaaatgccg tctgaacatt tcttcagacg gcatttgggg gttaagctga
    17401 catttcccgc cagcgtttga cttggaggcg tacctgttcg ggcgcggtgc cgcccaagtg
    17461 gttgcgcgcg ttgaggctgc cttcgggtgt cagaacgccg tacacgtcgt ctgaaatcag
    17521 cttgctgaag ccttgcaggg cttcgagcgg caattcgctc aaatcaacgc cggcttcatc
    17581 ggcgtggcgc acggcttggg caacgacttc gtgggcatcg cggaagggca tacctttttt
    17641 gaccagataa tccgccaagt cggtggcggt ggcaaaaccc tgcatcacgg cggcgcgcat
    17701 attgccgggt ttgacggtta cgccgcgcat catatcggcg taaatccgca gcgtgtcgat
    17761 gagcgtgtcg gcggtgtcaa acagcggttc tttgtcttcc tgattgtctt tgttgtatgc
    17821 caagggctgc gatttcatca gggtaatcag gccgataagg tgtccgatga ctcggccgga
    17881 tttaccgcgc acgagttcgg gtacgtcggg gtttttcttt tgcggcatga tggacgaacc
    17941 cgtacagaag cggtcggcga tgtcgataaa gccgaaacgc ggactcatcc acaaaatcaa
    18001 ttcttcagac aggcggctca agtgaaccat aaccagcgag gcggcggcgg tgaactcaat
    18061 ggcaaaatcg cggtcggata cggcatcgag cgagttctgg cagatttgtt cgaaacccaa
    18121 caattcggcg gtgatttcgc gctgaatcgg ataggtcgta ccggcaaggg cggcagcgcc
    18181 gagcggcata cggttgacgc gtttgcgaca gtcggccatg cgttcgaaat cgcgtccgag
    18241 catttcgacg taggcgagca tgtggtgtcc gaagctgacc ggctgggcga cttgcagatg
    18301 ggtaaaaccg ggcatgacgg tttcggcgtt ttgttccgcc aaatccaata atgccgtctg
    18361 aaggttttga atcaggtttt ggataacggt aatctggtcg cgcagccaaa ggcggatgtc
    18421 ggtggcgact tggtcgttgc ggctgcggcc ggtgtgcagg cgtttgcccg cgtcgccgat
    18481 tttgtcggtc aggcggcgtt cgatgttcat gtggacatct tccaaatcga gcggccattc
    18541 gattttgccg ctgcggattt cttcgaggat ttccgacatg ccccgacgga tgtcggtcag
    18601 atcgttttca ctcagcacac ccgaccgggt cagcatctgc gcgtgggcga gcgagccttg
    18661 gatgtcccat tcggcaaggc gttggtcgaa accgatggag gcggtgtatt gtttgacgag
    18721 ttcggaaacg ggttcgttga aacgtccgga ccaggttttg tcgtgcataa ggattccttg
    18781 atggggttat tcggtgcggt atttttccaa aaggcggcgg aagggttcgc cggtttcggg
    18841 gtgttttaaa ccgtaggcga cggtggcttc gagatagccg agtttgctgc cgcagtcgta
    18901 gcgcgtgcct tcaaaggggt gcgccaagac aaattcgtga tcgagcagct tggcgatgcc
    18961 gtctgtaagc tggatttcgt tgcccgcgcc gcgcggcagg ccggttaaaa ggtcgaaaat
    19021 gcgcggggtg aggatgtagc gtccgacaac ggcaaggttg gagggcgcgt cttcgggctt
    19081 gggtttttcg acaatgccgg taatgcgttg gaactgtttg agctgttcgg tttcgacgat
    19141 gccgtatgag ccggtttgcg acggttcgac ggtttctacg cccaaaatgc tgttaccgct
    19201 gcgcccgtac acttcgacca tttgtttgag cgcgcctttg ggcgcatcga ttaagtcgtc
    19261 ggcaaggatg acggcgaagg gttcgtcgcc gatggcggcg cgggcgcaca agacggcgtg
    19321 tcccaatccc agtgcttccg cctgacggat gtagaggcag gtaatgttcg gcggcaggat
    19381 gttgcggacg tgttccaaca atttgtcttt atggcgcatt tccaacttag tttcgagttc
    19441 gtatgccttg tcgaaatggt cttcgatgct gcgcttgttg cgcccggtaa caaacaccat
    19501 ttccgtgcag ccggcttcca cggcttcttc tacggcgtat tggatcagcg gcttgtcgac
    19561 gatgggcagc atttctttcg ggttggcctt ggtggcgggc aggaagcggg ttcccatccc
    19621 tgcaacgggg aagacggctt tttttatcgg tttcattctt tttcctttgt attgttttga
    19681 tgtttaaagg gcgagtttgc ggattaaatc ggcgagtgcc tgcgcgcggt ggctttcgcg
    19741 gtttttgacc tccgaatcca attcggcggc ggttttgccg tgttcgggca gataaaaata
    19801 cgggtcgtaa ccgaaaccgt tttgcccgag cggcgtgtcg tgccattgtc cgtgccatat
    19861 gccctcggcg ataatcgggc gcgggtcgtc tttatgacgg acaaaaacca atacgcagac
    19921 ataagaacaa cttttgtcgg ccttgccggc aagttcggcg gcaagtttca ggttgttggc
    19981 ggtatcggat ttgggattgc tgccggcgta acgtgcggaa tggatgcccg gcgcgccgtt
    20041 tagggcggcg gcacagatgc cgctgtcgtc ggcgagtgcg ggcagcccgc tgtgtccggc
    20101 ggcgtgccgg gcttttgcca gcgcgttttc gacaaaggtg ggatagggtt cggggcattc
    20161 gggtatgccg aatggggatt gcggcaatac ggtgatgccg taaggtttga ataagttgcc
    20221 gaattctttg agcttgccgg cattgccgct tgccaaaacg attttttccg gtttttcaga
    20281 catagcggtt tccctgtgat gaagatgggg cggcgcgtag ggatttgtgc cgcaggtaga
    20341 gggcgagtgc gccgatttgc ccgaacagcg cgccgaatgc gaaaaggaag gacgcgacgg
    20401 cgaaggcttt gctgccggcg gtcagcagat aagcacccaa gaggacgggc agcataaatg
    20461 ccagtgtgaa caaggcaaca gacaacagat agatttttcg gcggttcatg gcgttcggtc
    20521 ggaaacggga tgttcggatt atagccgatt aggacggcat tccctagagg ctggaaaaat
    20581 gccgtctgaa gcggcgttca gacggcatgg cgggcattat gcctgtttgt tccaacgttc
    20641 gatggattct ttgatgactt ttttcgcttc ttccgcaccg ccccaggatt cgactttggt
    20701 cgtacccgct tttttcaggt ctttgtagtg gttgaagtgg aactcgattt gtttgatgag
    20761 ctgttgcggc aaatcggaca aagttttgta ggcgttgccg ttattgcggt cgtcggcagg
    20821 tacgcagacg attttgtcgt ccacttcgcc gtcgtcgacg aatttcatca cgccgataac
    20881 gcgcgcttcc aagaatacgc cggttgccag aggttgttcg gtaacgagca acacgtccaa
    20941 ttcatcgccg tcttcgtcca aagtttgggg gatgaagccg tagttggtcg gtttggcgaa
    21001 gatggcgggt tcgatgcggt cgagttggaa tgcggcgagt ttgcggttcc actcgatttt
    21061 gtggttgctg ccggcgggga tttcgttgac gacgttgatg atgccgccgt ccacgtcgcc
    21121 cggggtcagg atttggttga agtctgccat ttggtttcct ttatttggga aggtttgaag
    21181 tttgaaagta tagcacaaac gtccggctgg aaatgcgtcc gatgccgtct gaaagggaaa
    21241 ccgggcgcgt gttaccgttt gcccaaaaac ctgcccagtt ccaaaatcgc gcgcctgttg
    21301 gacggggaaa acactttttc cgccgcttct tccaagccga accagccgta ggagacgtgt
    21361 tcctcgggtt gcaggacgac gggcgtatcg cgcgggattt cggcccggaa gacgtgttcg
    21421 cggttttcaa acacgccttt ggggtagcgg tgccgccagt ggtggtagat ttcgtaaacc
    21481 gtgctgtcgt gccggtcttg gagctgcccg tcctccagca ggatgccggt ttcttcccaa
    21541 acttcgcgcc ttgccgtttg ggcgacggtt tcgcccggtt cgaggctgcc ggttaccgac
    21601 tgccaaaatc ctttcggatg cgtgcgttcg atgagcagga tgccgccgtc cccgctataa
    21661 aggacgacca gtgcggaaac ggggtatttg agcggttttg ccatcggcat ctttcggcgg
    21721 gctgcggtaa tgaaggggcg gattatagca aacgccgcac gttatggcgt ttatcctttt
    21781 ccgtatcctt ttttctgcac ggatgggacg cgccggtgtt tgccggtaaa ttttcctttg
    21841 tgtcaaaaag ataagggcgg ttgtgatttt aatgcttgcc aaagtgacgg gcggaaacta
    21901 taatccgcga cttaccgagt cggagtgtgg cgcagtctgg tagcgcactt gcatggggtg
    21961 caaggggtcg aaggttcgaa tcctttcact ccgaccaaaa attccgaaag ccgctttcaa
    22021 aagcggcttt tttgccgtcc gtatgattat gatgtagagt acgcggcgac agacattcaa
    22081 atgccgtccg aaaaccgttc agacggcatc tctttatctt
//
LOCUS       DAWTCI010000025        21807 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWTCI010000025 DAWTCI010000000
VERSION     DAWTCI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21807
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.025"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(85..735)
                     /gene="pgmB"
                     /locus_tag="WM481_000982"
     CDS             complement(85..735)
                     /gene="pgmB"
                     /locus_tag="WM481_000982"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFA9080321.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYLAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGETEFAELTCRKNDNYLEMIQAVKPEDVYPGILPLLEA
                     LRANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPSPDIFLAAAEGVGA
                     DIRRCRRRRHQSLWRFAHRRGQNRRLGQRHRAGVRHRRADLRLLAKCVGTVGQIKF"
     gene            complement(748..3005)
                     /locus_tag="WM481_000983"
                     /pseudo
     CDS             complement(748..3005)
                     /locus_tag="WM481_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
     gene            complement(3252..4213)
                     /locus_tag="WM481_000984"
                     /pseudo
     CDS             complement(3252..4213)
                     /locus_tag="WM481_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(4217..4621)
                     /locus_tag="WM481_000985"
     CDS             complement(4217..4621)
                     /locus_tag="WM481_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFA9080322.1"
                     /translation="MICSFILAKVPNKYHKAGYFGCLALGALGFFSIFFIYNQYALIL
                     SYILIGIAWAGIITYPLTIVANALSGKHMDTYLGLFNGSVCMPQIVASLLSFVLFPML
                     GGHQATMFLVAGAVLLLGAFSVCLIKETHGGV"
     gene            complement(4634..5653)
                     /locus_tag="WM481_000986"
     CDS             complement(4634..5653)
                     /locus_tag="WM481_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9080323.1"
                     /translation="MIGDRRAGNHFGFSKANTFQIKKKDLLYVGIYASNSKTRFARAG
                     KKHDLDVELRLSRRSDGLYPAKLADEPHFSNARRRPAQFGLVFHPAAAGGDAGSADSG
                     YYSDRTWKPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIAL
                     LDVSSNMAMQPFKMMVGDMVNEEQKSYAYGIQSFLANTDAVVAAILPFVFAYIGLANT
                     AEKGVVPQTVVVAFYVGAALLIITSAFTISKVKEYDPETYARYHGIDVAANQEKANWF
                     ELLKTAPKVFWTVTPVQFFCWFAFRYMWTYSAGAIAENVWHTTDASSVGHQEAGNRYG
                     VLAAV"
     gene            5807..7483
                     /gene="ettA"
                     /locus_tag="WM481_000987"
     CDS             5807..7483
                     /gene="ettA"
                     /locus_tag="WM481_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFA9080324.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGT
                     GKSTVLRIMAGVDKEFEGEAVPMGDIKIGYLPQEPELDPEKTVREEVESGFGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKPKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIARQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
     gene            complement(7543..8028)
                     /locus_tag="WM481_000988"
     CDS             complement(7543..8028)
                     /locus_tag="WM481_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFA9080325.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGAFGTLAALPLA
                     FVLILLGIDGLLLAFVCIVLFMWGIRICACAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPPPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(8021..8977)
                     /gene="thiL"
                     /locus_tag="WM481_000989"
     CDS             complement(8021..8977)
                     /gene="thiL"
                     /locus_tag="WM481_000989"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFA9080326.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEGLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTCIGKINGGCRLKVLDAGGRELELHSLGFDHFG"
     gene            complement(9752..10462)
                     /gene="rmpM"
                     /locus_tag="WM481_000990"
     CDS             complement(9752..10462)
                     /gene="rmpM"
                     /locus_tag="WM481_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFA9080327.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAVPEPEPAPVAVVEQAPQYVDETISLSAKTLFGFD
                     KDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSEKYNQALSERRAYVVANNLV
                     SNGVPASRISAVGLGESQAQMTQVCQAEVAKLGAKASKAKKREALIACIEPDRRVDVK
                     IRSIVTRQVVPARNHHQH"
     gene            10826..11776
                     /locus_tag="WM481_000991"
     CDS             10826..11776
                     /locus_tag="WM481_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFA9080328.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFAKARLE
                     RPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(11889..12623)
                     /gene="fnr"
                     /locus_tag="WM481_000992"
     CDS             complement(11889..12623)
                     /gene="fnr"
                     /locus_tag="WM481_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFA9080329.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNEFSQLDAVIRQSR
                     RLKKGEYLFRAGGAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSYV
                     HSCDAVALENSEVCELPFTHIEELGQNIPSLRTHFFRMISREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            12758..14179
                     /gene="hemN"
                     /locus_tag="WM481_000993"
     CDS             12758..14179
                     /gene="hemN"
                     /locus_tag="WM481_000993"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFA9080330.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKVLHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADTYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPSGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSVGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(14217..14405)
                     /locus_tag="WM481_000994"
     CDS             complement(14217..14405)
                     /locus_tag="WM481_000994"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080331.1"
                     /translation="MPSEPVFRRHFVDGMCGLVPYAQSVLSGYAVWYLGFGTVGHKGY
                     DMPPPVLENTYPRQQNAV"
     gene            complement(14419..15993)
                     /locus_tag="WM481_000995"
     CDS             complement(14419..15993)
                     /locus_tag="WM481_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFA9080332.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTD
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASRKGLPVSTTHAIIGGIVGSA
                     LCMAFTHNADGVALIRWGKLGEIGMSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGTMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQSAVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSMASYLILQAVF"
     gene            16318..17418
                     /locus_tag="WM481_000996"
     CDS             16318..17418
                     /locus_tag="WM481_000996"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFA9080333.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRVVLNIGGIANISVLPPGAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLGRLLAHPYFSQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVC
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            17537..18391
                     /locus_tag="WM481_000997"
     CDS             17537..18391
                     /locus_tag="WM481_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9080334.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPALKGRKAALYVSVMGDQGSGNI
                     SGGRYSIDALIRGGYHNNPDSATRYSYPAYDTTATTKSDALSGVTTSTSVLNAPAAAL
                     TKNNGRKGERSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVVPPEYA
                     DTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTAAYESQ
                     YQEQYALWMGPYSVGKTVKASDRLMVDFSDITAYGDTTAQNRPDFKQNNGKNPDVGNE
                     VIRRRKGG"
     gene            18395..20125
                     /gene="mafB"
                     /locus_tag="WM481_000998"
     CDS             18395..20125
                     /gene="mafB"
                     /locus_tag="WM481_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFA9080335.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNTQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     HEEHAPFDNHAADSASEEKGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRRGNSMEFVNGVAAGALNPFISAGEALGIGDILYGTGYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSAAGFEKNTREAVDRWIQENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKA
                     AVSGDFSKSYTCSFHGSTLVRTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYG
                     NPYQETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLFAENGAEQTVQ
                     SVTVKPEPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPPKPKPTNHAQQRKEEAK
                     NDSHRSVGDSNRVVREGKQYLDSDTGNHVYVKGDKVVILTPDGRQVTQFKNSKANTSK
                     RVKNGKWTPK"
     gene            20103..20384
                     /locus_tag="WM481_000999"
     CDS             20103..20384
                     /locus_tag="WM481_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080336.1"
                     /translation="MGNGHQNNQSSLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEKAISHHLEDFS"
     gene            20440..21087
                     /locus_tag="WM481_001000"
                     /pseudo
     CDS             20440..21087
                     /locus_tag="WM481_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147002.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            21084..21500
                     /locus_tag="WM481_001001"
     CDS             21084..21500
                     /locus_tag="WM481_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080337.1"
                     /translation="MKTSTIVFGGFFITDNGERIQIPILENPNIKEINNFFSVSNFEK
                     KAGVLVFRIIPEPEFGNTELTIYFEKGYYLPIIQTILEDGDIEVKNLKTENYSGNTME
                     ILGDVYPIEHISKNISIIQDIISEFIMKNKPITIMI"
     gene            21603..21807
                     /locus_tag="WM481_001002"
                     /pseudo
     CDS             21603..21807
                     /locus_tag="WM481_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
BASE COUNT         5599 a         6039 c         5492 g         4677 t
ORIGIN      
        1 tttttgcaaa ggtctcgggt tatcattctg ttctaaaagc agaaaggccg tctgaaacac
       61 tgttcagacg gcctttttac ttccttagaa ttttatctgc ccgactgttc ccacacattt
      121 tgcaagtagg cgtaggtcag ctcggcggtg tcggacacca gcgcgatgtc gctgcccaag
      181 tcttcggttt tgcccacgcc gatgggcaaa gcgccagagg ctttgatggc ggcgacgccg
      241 gcagcggcgt atgtccgcac ccacgccctc ggcggcggca aggaagatgt cgggggaggg
      301 tttggaatgc gcgacggcgg cagggtcggc gacggtgtcg aagaagtggg tcagccccat
      361 gcgttcgagc aagaacgggc cgtttttact ggcggacgcg agggcgattt ttttgccgtt
      421 tgccctcaat gcttccagca gcggcaaaat gccggggtac acgtcttcgg gtttgactgc
      481 ctgaatcatc tctaagtagt tgtcgttttt gcggcaggtc agttcggcga actcggtttc
      541 gccgacggtt ttgccgccgt gcgcgaggat gcgtttgagc gaatcgtcgc gcgacacgcc
      601 tttgagctgc tcgttaaact tgcggtcgat gccgatgccc aattcttcgg caagcttttt
      661 ccatgcgagg tagtggtatt cggcggtatc ggtaatcacg ccgtcgagat cgaagagcac
      721 tgcggtaaaa gtcattttgc gccctcctta tttttccaat gcaacggtgt ggctgccgtt
      781 gagcgtgata tctttgccgt acacctgcaa ttcgagcgga tcgcctttga gcagggtgaa
      841 gacgacgttt tttgccgacg gcgactttaa tcagacggcc gcggtagttg atgtggaagg
      901 catagcctgt ccacgcactc ggcaggaacg gtgcgaagct gagtttgccg ccccaagttt
      961 tcatttgggc gaaaccttgg acgatggcga gccacgagcc ggtcatagaa gtaatatgca
     1021 ggccgtcttc ggtgtcgttg ttgtagtcgt ccaagtccag gcgggcggtg cgccggtaca
     1081 tttccacggc tttttcttct ttgcccagtt cggcagcgag gatggagtgg atacatggag
     1141 acaacgagct ttcatgcacg gtcatcggtt cgtagaagtc gaagttgcgg cgtttttcgt
     1201 ccatgtcgaa acggtcgccg aagaagtaga tgccttgcaa tacgtccgcc tgtttgatga
     1261 agggcgaacg caggattttg tcccacgacc atttctggtt gagcggcaaa tcgtcgggcg
     1321 aaagcgcgga tacggggcgg atgtctttgt caaggaagcc gtcgtgctgc acgaatacgc
     1381 cgagttcttc gtcatgcgga cggtacatat tcgcgctgat gtccgcccat ttttccaact
     1441 cggcggcgct cacgttcgaa cccggacgcg ggtatttcgc caaggcttcg cgggtgtagc
     1501 ccaataccca tgcggcgagg gtgttggttt accagttgtt gttgatgttg ttttcgtatt
     1561 cgttcggacc ggttacgcct tgaatcatgt atttgccgtt gcgtttggag aagtggacgc
     1621 ggtccgcccg gaagcgggac acttcgacca agacttccaa gccttctttg gcaagatagc
     1681 actcgtcacc ggtgtagttg gtgtagttgt agatggcgta aggaatcgcg ccgttacggt
     1741 ggatttcctc gaaggtgatt tcccattcgt tgtggcactc gatgcccgta aacgttacca
     1801 tcggatagag tgcgcccgcc aagccctgtt cgcgtgcgtt atgctgcacc tgcggcaatt
     1861 tggttgcggc ggtattgcag caggttgcgg gtaacttcgg gttccgccag tgcaaggtag
     1921 agcggcacgg cgtaggcttc ggtgtcccaa taggtcgcgc cgccgtattt ttcgccggta
     1981 aagcctttgg ggccgatgtt cagacgcgcg tcttcgccgt agtaggtgga gaacagttgg
     2041 aacaggttga agcggatgcc ctgctgtgct tcgtcgctgc cttcgatgac cacgtcggcg
     2101 atttcccaac gatgctgcca gcctgctttg tgcgcatcca gcaaggtttc aaacgcaaca
     2161 cctgcgattt tttcagataa ggcgcggccg gcggctttca ctgcttccaa gccctgataa
     2221 tcgcggctgg tggtaacaat cacgcgtttt tcaaaggttt cgggcgtgcc gccgacttcg
     2281 gcttcaaaag aattagagac ctgccaatcg gtttggctgc cgccgaaggc tttgaagctg
     2341 ccggcgaagg tttgcccggc gttgacgata aattgttcca caccgaaagg gttggcgacg
     2401 gtttgggcgg caatgtagga gcggtcgtct gaaacgcctt tgtccaatac ctgccagaat
     2461 ttttcttcgt agttggagtc ttcgtttttc acgtcggcat cgatgatgga atcgatgcgg
     2521 acttggtggg ttttgccgtc aacggacacg gcttcccagc gggtgagagc cagctctttt
     2581 tgcgcgaccg acaggaattt gcacacatcg aaacgcacac cgaatacggt gaacgagcgg
     2641 cgcaacacgc cgtgttgcat atccagttcg acggagaagc cggcaacgtc gtttttcgcc
     2701 aagtccacct cttgcccgtc gacaaagatt ttgactttgc tgaaattgag cgcgttgatg
     2761 gctttgccga aatatttggg atagccgttt ttccaccagc cgacgcgggt tttgtcggga
     2821 aaccacacgc cggcgatgta ggtgcccaag tggctgtcgg cggagtaggt ttcctcaaag
     2881 ctgccgcgca tacccatata gccgttgccc aagctggtca ggctctcttg cagccgtttg
     2941 tgttcttttt ccagttttgc cgaacgcagc gtccaagggc tgatttccat aattcttgtg
     3001 tacatcgtaa agctcctgtt tggattgatt tgagggaatg gtgaaatctt atagtggatt
     3061 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gcaaggcgtg
     3121 gattaacaaa aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg
     3181 cgaggcaacg ccgtactggt ttttgttaat ccactataaa aaggtcgtct gaaaccggtg
     3241 ttaggaagct cctaagaaag ggattcgatg ccgtaagcaa tcgtcgcctc cctagtatca
     3301 cccttgttca gacggatatt gccgaattcg ggccaatgca ggctgtcggg cagcgtctgc
     3361 gcctcggtcg ccagcgcgtc gtaaacgccc gcatcgtgcc gcgcgaaatc ctgcggggcg
     3421 gcggtaaaga tgaccaagcc gttgcggtcg ctgtatatgc tgatacgacg gcagcgtccg
     3481 gcttgcaaca cggcggcggg acggcctata tcggacggca cgcggtaagc gtcgtcaaaa
     3541 ccggcccgtt tcgcggcgca gggcggcaac ggcggcatcc ggcggcttgg gccggctgaa
     3601 atcaaatact tcgaggccgt ctgaaaccgt taagacgggc agtttttcgg catcggccgg
     3661 aatatgtccg ccctgcggaa tatgcagaac cgcatcgtgc aggcccgcgt ccagccgcca
     3721 gtaaatgtgc agcgtcgggt cgaacaccgt gtcgccgagc gcggtggcgc gataggtaac
     3781 ggtaagccgg ccatcctcgt ccaagcggta ggaaatatcc aaatcgttgg gataaccgtc
     3841 ggccgtctgc cgccaccgcg ttgaaacggg taacggccag cccgtgcgaa ccgccgtgca
     3901 gcgcgttcct gccttcgttg gcctccacgc ggtaagtcct accgttgatg tcgaacgccg
     3961 caccgcggat gcgtccggcc acgcgcccta tctgcttgtt aatctgaaac ggattgtccg
     4021 cataggaagc cgcatcgtcg aacgacacca cggggttttc gcgcacgccg tctgccaaaa
     4081 cggaaaattc ctgcacaatc ccgcccaagt ccagcacgaa gacgcacgta ccacgccggt
     4141 tggacagcac atagccggtt acggcccgcc cgtcgatcag gccgaaatcg cgggtagcgg
     4201 gggtatcgct catcgctcaa accccgccgt gggtctcttt aatcagacag actgagaagg
     4261 ctcccagcag caagactgcg cctgcaacca agaacatggt tgcctgatgg ccgcccagca
     4321 tcgggaaaag cacgaaactc aacagcgaag cgacgatttg cggcatacag acagagccgt
     4381 taaacaggcc caaataagta tccatgtgtt tgcccgacaa agcgttggcc acaatcgtca
     4441 gcggataagt gataatgccc gcccaagcga tgccgattaa gatataagac aggatgagtg
     4501 cgtattgatt gtagatgaag aagatagaga agaaaccgag cgcgcccaaa gccaaacagc
     4561 cgaaataacc cgccttatgg tatttattcg gtacttttgc cagaataaac gaacaaatca
     4621 ccgccgcaac cgactacacc gccgccaaaa cgccgtaccg gttgcccgcc tcctgatggc
     4681 ctacggaaga cgcatcggta gtgtgccaga cgttttctgc aatcgcgcct gccgagtaag
     4741 tccacatata ccggaaggcg aaccagcaga aaaactgtac cggagtaacc gtccaaaaca
     4801 ctttaggcgc ggtttttaag agttcgaacc agttggcttt ttcctgattc gcggcgacat
     4861 cgatgccgtg gtaacgggcg taggtttccg ggtcgtattc tttgactttg gagattgtga
     4921 acgcactggt aataatcagt aacgccgcac ccacatagaa tgctacgacc acggtttgtg
     4981 gcacaacgcc tttctcggca gtgttcgcca aaccgatata cgcgaacaca aacggcagaa
     5041 tcgctgccac aaccgcgtcc gtattcgcta agaaactttg aatcccgtag gcgtagcttt
     5101 tctgctcctc gttgaccata tcgccgacca tcatcttaaa cggctgcatc gccatattcg
     5161 acgacacgtc caacagcgca atcatcagcg cgccgaacga caaggccgcc agcgacgcat
     5221 agccgaaacc gaagctgccc gagttcggca tcaaaatcat cacgatgacc gcaatcagcg
     5281 tgccgtaaag cagatacggc aggcggcggc cgcccaagcg cggcttccaa gtgcggtctg
     5341 agtagtagcc actatcggct gaaccagcat ccccgccagc ggcggcagga tgaaaaacca
     5401 gcccaaattg tgcgggtctg cgcctagcgt ttgaaaaatg cggctcatct gcgagctttg
     5461 cagggtaaag gccgtctgaa cgccgagata gccgaagctc aacatccaaa tcgtgctttt
     5521 tgccggcgcg ggcaaacctt gttttgctgt ttgaggcgta tattccgaca taaagtaaat
     5581 cctttttttt gatttgaaaa gtatttgctt tggaaaatcc gaaatggttg ccggcgcggc
     5641 gatcccctat cattattatt tttttgtctg tataatttca aagggatagg cggattttat
     5701 gaatcctgcc cgattttggc aataccggtt cgcggataaa ctggcttaaa tcaaattatc
     5761 ggttaaaatg gccgtctgaa atttgtttga ccgaatcgag aaaactatgt cccaacaata
     5821 cgtctattct atgctgcgcg tgagcaaggt tgtgccgccg cagaaaacca tcattaaaga
     5881 tatttccctt tctttcttcc ccggcgcgaa aatcggcctc ttgggcttga acggcacggg
     5941 caagtccacc gtgctgcgga ttatggcggg cgtggataaa gaatttgagg gcgaagccgt
     6001 gccgatgggc gacatcaaaa tcggctatct gccgcaagag ccggagcttg atccggaaaa
     6061 aaccgtgcgc gaggaagtgg aaagcggttt cggcgaagtg gctgccgcac agaaacgttt
     6121 ggaagaagtg tatgccgagt acgccaatcc cgatgcggat tttgacgcgc tggcggaaga
     6181 acaaggccgc ttggaagcga ttatcgcggc gggttcgtca actggcggcg gtgcggaaca
     6241 cgaattggaa atcgctgccg acgcgctgcg cctgcctgat tgggatgcca aaatcggcaa
     6301 tctttcgggc ggtgaaaaac gccgcgtcgc tttgtgcaaa cttttgttga gcaagcccga
     6361 tatgctgctc ttggacgagc cgaccaacca cttggatgcg gaatcggtgg aatggctgga
     6421 gcaattcctc gtgcgcttcc ccggcacagt ggtcgcggta acgcacgacc gctacttcct
     6481 cgacaacgcc gccgaatgga ttttggaact cgaccgcgga cacggcattc cgtggaaagg
     6541 caattactcg tcttggctgg agcagaaaga aaaacgcttg gaaaacgagg cgaaatccga
     6601 agccgcgcgc gtgaaggcga tgaagcagga attggaatgg gtgcgccaaa atgccaaagg
     6661 ccgccaagcc aagcccaaag cgcgtttggc gcgttttgaa gaaatgagca actacgaata
     6721 ccaaaaacgc aacgaaactc aggaaatctt tatccctgtt gccgagcgtt tgggtaacga
     6781 agtgattgaa tttgtgaatg tttccaaatc gttcggcgat aaagtgctga ttgacgattt
     6841 gagcttcaaa gtgccggcgg gcgcgattgt cggcatcatc ggcccgaacg gcgcgggtaa
     6901 atcgacgctg ttcaaaatga ttgcgggcaa agagcagccc gattcgggcg aagtgaaaat
     6961 cgggcaaacc gtgaaaatga gcttgattga ccaaagccgc gaaggtttgc aaaacgacaa
     7021 aaccgtgttc gacaacattg ccgaaggtcg cgatattttg caggtcggac agtttgaaat
     7081 ccccgcccgc caatatttgg gacgcttcaa ctttaaaggc agcgaccaaa gcaaaatcgc
     7141 aaggcagctt tccggcggcg aacgcggccg tctgcacttg gcaaaaacct tgttgggcgg
     7201 cggcaatgtg ttgctgctgg acgaaccgtc caacgatctc gacgtggaaa ccctgcgcgc
     7261 gttggaagac gcattgttgg aatttgccgg cagcgtgatg gtgatttcgc acgaccgctg
     7321 gtttctcgac cgcatagcca cgcatatctt ggcgtgtgaa ggcgactcca aatgggtgtt
     7381 cttcgacggc aactatcaag aatacgaagc cgacaagaaa cgccgactcg gcgaagaagg
     7441 cgcgaaaccg aaacgcatca aatacaaacc ggtaacgcgt taacctccga aacaatgccg
     7501 tctgaaaggc tttcagacgg catttttaca aggcagcccc gtttaaaaca gcattgcaat
     7561 cctcaagaca atcaaggtca tcaccgcagc cgccatatcg tccgccataa tgcccaaacc
     7621 gccgtgcaga ttcttgtcaa accaaccgac ggggggcggt ttgagcgcat caaacagacg
     7681 gaacagaaca aatgccgcca gccaccacgt ccacctgaac ggcacaaacg ccagcacaaa
     7741 cagcatggcg acaatctcgt cccaaacaat cccaccgtgg tcactgacac ccgtttcacg
     7801 ttccgcacac gcacaaatac gtatgcccca cataaacagc acgatacaca caaaagccag
     7861 cagcagcccg tctatgccga gcaaaatcag cacaaacgcc aaaggcagtg ccgccaaagt
     7921 gccgaatgcg cccggcgcaa acggagccag cccgctgccg aaaccgaaag ccaaaaaaca
     7981 caacggccgt ttcaacagcc acgcaaagtc aggtttaaaa tcagccaaaa tgatcgaatc
     8041 ctaaagaatg tagttccaat tccctgccgc cggcatctaa aaccttcaga cggcatcctc
     8101 cgttgatttt gccgatgcac gttaccggca cgccgcaccg ttccgccgca tcaagcacgc
     8161 ggctgcggca actttccgga gcggtaaaaa ccagctcgta atcgtcgccg cccgccaaag
     8221 tataagacag ccattgcgct cggggcaaaa tatctttcaa tacagataaa gatggcagcg
     8281 aatcggccca aatttccgcc cccacgccgg aagcggtcag gatatgcccc aaatcttgcg
     8341 caaggccgtc tgaaacatcc tgtgccgccc gggcaaacgg caacagcgca agccccagcc
     8401 caacccttgg ctcgggacgg agcagctttt gttcgcattc ggcgaacaca tcatccggca
     8461 acacacaccg tttcagacgg cagttcaaag ccgccgccgc cataccgaca cgccccgaca
     8521 cccaaatatc gtcgcccgca accgccgcat cacgccgcaa cgccctaccc ttcggcaatt
     8581 cgccgataat ggttacattg aacgccatat cgcccttggt cgtatcgccg ccgattaacg
     8641 ttacgccaaa cttttttgcc aaaccgaaaa agctgccgca aaaccgttcc agccatactt
     8701 catccaattc gggcaacgcc gcgctcagca gcacccaacg cggtatcgca cccatcgccg
     8761 ccatatctga aatattgacg gccaaaacct tccaagccaa gccttcaggt ttgacatctg
     8821 caaaaaaatg cctgtccttc aaaagcatat ccgcactgaa acacaaatcg aagccttcgc
     8881 gcgggcggac aatcgccgca tcgtcgccta tgcccaatac gacatcctta tccgtacctg
     8941 tttgcaaata ccgtttgatg aagtcgaatt ctttcatatc ccaaaatatc cggcgtgcga
     9001 atccaattta aatcatagca aagcagcaaa ggtcgtacaa acaataaagc ggctcattca
     9061 gtgtttcagc gtatttcact tttagcttta gccataccgt acccgtatgc accgaccacc
     9121 ctgtttcaac acacaaacgg catatcaaag caccgcactg cacgtcgccc cgaatgcaaa
     9181 gcggcaactt ccgccaccga ttaggctcaa tcgctgcaac tggcggggtt tcaaccgaaa
     9241 agggatgatg ataaaaccat atccggcata cagtgccgca cacatcgggc acgattccat
     9301 ccatttccca atgaggggga aaggcgtgaa tttgtatgaa ttatcccttt cggggcttga
     9361 tacagaaagg attcccttgc tatgggcaaa cccacatcct accgccttaa aatcagagtt
     9421 tcaaccgata tagtggatta aatttaaacc ggtacggcgt tgcctcgcct tgccgtacta
     9481 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataaagcgcg
     9541 gcaatgggac acaaacatga atctgaatcc gaatccgaac ctgcaccccg attctgacga
     9601 tagccgtttc agaaaaccac ccaaaaaaac aaaccccgca aaagcggggt ttgtttcaca
     9661 tcatcgatac cggccgacgt ttcacgaata ttgcaacagt acccggtaca aaatccgcta
     9721 acctcatgca tcggcaagat attacctagc cttagtgttg gtgatgattg cgtgccggca
     9781 caacctgacg ggttacgatg ctgcggattt tcacatctac gcggcggtca ggttcgatac
     9841 atgcaatcag agcctcacgt tttttggctt tagaggcttt cgcacccagt ttggcaactt
     9901 cggcttgaca aacttgagtc atttgcgctt gagattcgcc caagccgaca gcagaaattc
     9961 tagaagcagg tacgccgttg ctgaccaggt tgtttgccac tacgtatgcg cggcgttcgg
    10021 acagagcctg attgtatttt tcagaaccca taaagtcggt atggccttcg acgcggacag
    10081 attggacatt ggttcgactc aggcgttgcg ccaatacttt caggttgtct tgagcttcgg
    10141 cgcgcaatga atccttatcg aaaccgaaca gggttttggc agacagggaa atggtttcat
    10201 caacatattg aggagcctgc tccacaacgg cgacaggcgc gggttcgggc tcggggacgg
    10261 caaccgcatc gccgcattct acgcgacctt ggcttgcttt atcaaagtag gcgtttttcc
    10321 agcattctcc atagttgttg cgtacgattt cgttcgactg gccgcttacg gtgtaaccct
    10381 gaacggacgc ctcgcccgca acagcagtgc cggaagcgag caatgcaacg aataatgcgc
    10441 ttaatttcag ctgtttggtc attttattcc ctcattagat ttgtacagca gatacagaag
    10501 caactgctga attgccaaca tacaggccgc tgtagaaaca tggcggcaaa tcggatattt
    10561 cagtctccac tataaagaaa cagggggcgg actgtcaata ctcaacgcct gaaaacaacc
    10621 gtaaaacagc ctgtttcctg atagtctatc atgccgaaaa atcaattccg tgtcattact
    10681 ttagggggat tttttccata tcgcacgatt gccgttgcac aaaaacaaca aacacaacgg
    10741 caaagcccca taccgtaccg gcaagaaaat atgataaatt ataaacaatg ttcagccacc
    10801 cgacacagac ccacaccgac ccgccatgaa attacaacaa ttgaaatacg ccttagaagt
    10861 ttaccggcac aacctgaacg tttccgaagc ggccgaagcc ttattcacat cacaacccgg
    10921 catctccaaa caaatcaaat tgctggaaga agaaatcggc attcagattt ttatccgcag
    10981 cggcaagcgc gtggtttcgg tctcgcagcc gggcaaggtg gttttggata ttgcggaacg
    11041 tattttgcgc gatgtacaga acattaaaaa tatcggcagc gagtttaccg gacaggacag
    11101 cggttcgctg acggttgcca cgacgcatac gcaggcgcgc tatgccctac cgttgattgt
    11161 tgccgatttt gtgaaacgct atccgaaagt caatctgacc atcaaacagg gaagccctgc
    11221 cgccatcgcc caaatggtta cttcgggaga atctgatttg gcgattgtta cggaacggat
    11281 agacgaccac cccgagttgg gaaggctttc ctgctacgac tggacacacg cggtgattgt
    11341 gccgaacgac catccgctgc tcgaatgcag gaatcccctc cgtattgaag atttggcgag
    11401 gtttccgctg attacttatg aatttgcatt caatgcgggc agcagcatcg cgcgggcatt
    11461 cgccaaagcc cgtttggaac ggccggatgt cgcattggcg gcggcggata cggatgtatt
    11521 gaagacttat gtgcgcttgg gtttgggcgt gggactgatg gcgaaaatgg catacaaccc
    11581 ggatacggac ggcgatttgc agcttgtgga tgcggcacac ctgttcgagc cgtcgccgac
    11641 gtggatagct ttgcgcagcg atacttattt gcgcggatat gcctacgact ttatccaagc
    11701 gtttgcgccg cacctgacac gcgagaaggt ggataggatt ctgtacacgc ccatcagcga
    11761 ggatttttcg atttaggcgg ctgccggttt tcaggcggca ctttgcggca gatacaacaa
    11821 acagggcaga tgttttcgtc tgccctgtgt ttattgagaa tgccgtctga aatgttcgta
    11881 caggttaatc aaatggcgtg cgaacaaccg gataccattt ttttcagcac ctgcagattg
    11941 agaatcttga tgtgtttgtg ttcgacggaa atcaatcctt cctgatggaa cttggataat
    12001 gtgcggctga cggtttcaag tttcagcccg agataactgc cgatttcttc gcgggacatt
    12061 cttaagatga agtcgttggc ggcgaaacct cgggaataaa ggcgttggga aaggttcaac
    12121 aggaaggcgg caatccgctc ttcggcgcgc atattgccca acaacagcat aacaccttga
    12181 tcgcgcacaa tttcgcggct tatcatgcgg aaaaagtgtg tacgcaggct ggggatattc
    12241 tgaccgagct cttcgatatg ggtaaacggc aactcgcata cctcgctgtt ttccaaggcg
    12301 accgcgtcgc aactgtgcac ataggaacag atgccgtcca tgccgatgag ttcgcccgac
    12361 ataaagaaac ccgttacctg atcgcggccg tcctgactgg cgacggttgt tttgaagaag
    12421 cccgaacgga tggcaaagag cgaggtaaag gctccgccgg cacggaacag gtattcgcct
    12481 tttttcaggc ggcggctttg gcggatgacg gcatcgagtt ggctgaactc gttgggcagc
    12541 agcccgacag gcaggcagag ttcccgcaaa gaacaggaag aacacagcgt tttcatctga
    12601 tgtgtagtat tatgcgaagc cataccgtac cttttcgtgt gttttgcccc atcccgatta
    12661 tgtgcttgtt gacacatatc aagacaggtt tatcatactg tggcattcta ccaaactatc
    12721 caaaatttga ctaacatcat aaaaccgcac acacaccatg aaaatcattc agatacagaa
    12781 caatcacaat gtaaacgatg accgccccga gtttgaccgc gcgctgattg ccagcctgcc
    12841 cgccagcggt ccgcgctata cttcctaccc taccgccgac cgtttccatg acggtttccg
    12901 cgaaggcgaa tatatcaaag ttttacattt gcgcggtatg ggcgcgttaa acaaaccgct
    12961 ttccctttac attcacattc cgttttgcaa caccatctgc tactactgcg gctgcaacaa
    13021 aatcatcacc aaagacaaaa gccgcgccga tacctacatc gaatatcttg aaaaagaaat
    13081 ggaactgctc gctccacatc tgaacggacg gcaccagctt gcccaactgc acttcggcgg
    13141 cggcacaccg acctttttga gcgacgaaca gatcgaacgt gtcttccgca tgatccgcaa
    13201 acatttcgaa ctgataccgt ccggcgaata ttccatcgaa atcgacccgc gcaaagtcag
    13261 ccgcgacacc gtcctcatgc tcggcagact cggcttcaac cgcatgagcg tcggcattca
    13321 ggatttcgac cccaaagtgc aggcggcagt caaccgcatc caaagctacg aagaaaccaa
    13381 agaagtcatc gatgccgccc gtgaggcagg gttcaaatcc gtcagcgtcg atttgattta
    13441 cggcttgccg catcagactt ccgaaagcat caaaaccacc atagataccg ttttgtcgct
    13501 cgatcccgac cgcctcgccc tttaccacta cgcccacctg ccgcacgtgt tcaaaccgca
    13561 acgccgcatc gataccgccg ccgtccccga cagcgaagag aagctcgata tgctgcaata
    13621 ctgcgtccaa accctgacag agcgcggcta cgtcttcatc ggcatggacc atttcgccaa
    13681 acctgacgac gaactctcca tcgccctcaa agaaggcttc ctccaacgca acttccaagg
    13741 ctattcgacc tacgcggatt gcgatttggt cgccatcggc gtgtcgtcca tcggcaaaat
    13801 cggcagcacc tattcccaaa acgaacgcga catcgatgcc tactatgccg ccatcgacga
    13861 aggcagactg cccatcatgc gcggctacca gctcaatcag gacgacatcc tgcgccgcaa
    13921 catcattcag gatttaatgt gccgcttcgc gctcgactac aggatttacg aaagtatgtt
    13981 cggcatcccg ttcgaccgct acttcaaaga cgaactggcg gatttggaaa aactcgccgg
    14041 tttgggattg gtgcgcctga acagccacgg gctgaccgtt acaccgaaag gacgcttcct
    14101 catccgcaac atcgccatgg tcttcgacta ccacctgcgc cacaaagaaa ccaaagcgaa
    14161 atattcgcaa acggtgtgat tgcggctaac gtacaaaagc cgtctgaaag gctttttcag
    14221 acggcatttt gctgccgagg ataagtgttt tcaagaacag gcggcggcat atcataacct
    14281 ttgtgtccga ccgttccgaa accaagatac caaacagcat atcccgacag aacagattgt
    14341 gcataaggca caagcccgca cattccatca acaaaatgcc gtctgaacac gggttcagac
    14401 ggcatcagta ttttacaatc agaacactgc ctgcaaaatc aaatagctgg ccatcgacaa
    14461 tacagcggcg gcaggcaggg taatgaccca cgccaaaccg atgggtttca tcagtttcca
    14521 gttggcattg cggttgacca gaccgatacc gagtaccgcg ccgaccaaga tatgcgtact
    14581 ggacacgggc agccccatca gcgacgcgcc catcacgacg gatgcggcgg acagttcggc
    14641 ggtaaaaccc gaagcaggat gcatttccgc caaactcgta ccgacggttt taatcacctc
    14701 tttaccgaca aaccacaaac cgacaatcag cgcgatgccg aaagtcagca tcgcaatcgg
    14761 agggacggcg ctttgcgcgg caacgctgtt cgtccgcaaa acatccataa tcgcggcaaa
    14821 cgggccgatg gcgttggcga tatcgttcgc accgtggctg aatgcgaagc cgcaggcggt
    14881 aaagacctgc atccatgaaa acatctgaaa ggtcgatttg cccaagtctt tacgcttgag
    14941 gcttttggca aacacaaacg tccccatcca caccgccgcg cctatcataa agatggtcag
    15001 gaagctgttg acgttgctca tccccaaatg caggtttttc aagcccttga aaatcagcat
    15061 agcggaaatc atcatcgtgc cgaacgaagc gataaaggga atccaagaat gcagcgcctt
    15121 gtaggaatcg acattgtttt tacggttgtc gaacgcataa agaccgcggt aatactccga
    15181 ttgcagctct tgaggatcga attcgggttc gtcgtaaatt tgcgcgtcgt gcgccatttt
    15241 ggtggcgtac tcgacttttt cggcttcgga caaaccctcg aaaaacaggc ggtgccgttc
    15301 tttataggcc tttttttcct gcttgatgcc cttgagcgtt ccttccgccc aagcgttgta
    15361 atctaagacg tttttcttga cgcgcgaaaa caggaaatag gacaccgcgc cgcccaacac
    15421 gggcgacaat acccaagaca tcccgatttc gcccagcttg ccccaccgta tcaacgccac
    15481 cccgtccgca ttatgcgtaa acgccataca caacgcactg ccgacgatgc cgccgataat
    15541 cgcgtgcgtc gtcgaaaccg gcaggccttt gcgggacgcg aacagcagcc acaacgccgc
    15601 cgccaaaagc gcggacatca taataaacac aaactgtatg ggttcgaaat caacaccctt
    15661 caaatcgacg atgcctttgc gtatggtatc ggttacctcg ccgcccgcga tgaccgcgcc
    15721 gctgacctca aataccgccg caatcagcaa agcctgcggg atggtcagcg tgcctgcacc
    15781 gacgctggtg ccgaaagaat tggcaacatc gttgccgccg atgttgaacg ccataaacac
    15841 accgaacatc gtggcaataa tgaataaggc ggcgtaattg taatgggtat aacccaagcc
    15901 ccaataaata aaatagccga ctgtacctac cagcatggcg gcaaagacgg cgttgatggt
    15961 tttaacattt gcgctcattt ggatttgagc catggcaatc ttcctttaat gtggtggaaa
    16021 tcatgagtgt tgccgacact gtattgctgc ctccggtcga actgccgttt cgggatatgg
    16081 cagacgcatc cttccttctg aaccgcatcc gaataccccc aaacctgacc gaatgcaaaa
    16141 ctgctgccgg aaacggtttg tatgacactt ctactactgg atgttgcggg cgcattataa
    16201 tatttttcca cccgtcttaa aacatttatt tacactttat ttacactgcg gcggcaaatc
    16261 ggtatacgag cgtcaataca cgttaaaatg gcgttttgca ccagtttggg agtgatgatg
    16321 gaaacacagc tttacatcgg cattatgtcg ggaaccagta tggacggggc ggatgccgtg
    16381 ctggtacgga tggacggcgg caaatggctg ggcgcggaag ggcacgcctt taccccctac
    16441 cctgaccggt tgcgccgcaa attgctggat ttgcaggaca caggcacaga cgaactgcac
    16501 cgcagcagga tgttgtcgca agaactcagc cgcctgtacg cgcaaaccgc cgccgaactg
    16561 ctgtgcagtc aaaacctcgc tccgtgcgac attaccgccc tcggctgcca cgggcaaacc
    16621 gtccgacacg cgccggaaca cggttacagc atacagcttg ccgatttgcc gctgctggcg
    16681 gaactgacgc ggatttttac cgtcggcgac ttccgcagcc gcgaccttgc tgccggcgga
    16741 caaggtgcgc cgctcgtccc cgcctttcac gaagccctgt tccgcgatga cagggaaaca
    16801 cgcgtggtac tgaacatcgg cgggattgcc aacatcagcg tactcccccc cggcgcaccc
    16861 gccttcggct tcgacacagg gccgggcaat atgctgatgg acgcgtggac gcaggcacac
    16921 tggcagctgc cttacgacaa aaacggtgca aaggcggcac aaggcaacat attgccgcaa
    16981 ctgctcggca ggctgctcgc ccacccgtat ttctcacaac cccacccaaa aagcacgggg
    17041 cgcgaactgt ttgccctaaa ttggctcgaa acctaccttg acggcggcga aaaccgatac
    17101 gacgtattgc ggacgctttc ccgattcacc gcgcaaaccg tttgcgacgc cgtctcacac
    17161 gcagcggcag atgcccgtca aatgtacatt tgcggcggcg gcatccgcaa tcctgtttta
    17221 atggcggatt tggcagaatg tttcggcaca cgcgtttccc tgcacagcac cgccgaactg
    17281 aacctcgatc ctcaatgggt ggaggcggcc gcatttgcgt ggttggcggc gtgttggatt
    17341 aaccgcattc ccggtagtcc gcacaaagcg accggcgcat ccaaaccgtg tattctgggc
    17401 gcgggatatt attattgagc gcaaataccg tctgaaagcc atttccgccg ttcagacggc
    17461 atttccaccc ctttcgttta caaaccctta aaatccattt acactcaaac ccgttcaaaa
    17521 caaaacaaac cctgccatga aaaccctgct cctcctcatc cccctcgtac tcaccgcctg
    17581 cggcacactg accggcatac ccgccctgaa aggacgcaaa gccgcccttt acgtctccgt
    17641 tatgggcgac caaggttcgg gcaacataag cggcggacgc tactccatcg acgcactgat
    17701 acgcggcggc taccacaaca accccgacag cgccacccga tacagctacc ccgcctatga
    17761 cactaccgcc accaccaaat ccgacgcgct ctccggcgta accacttcca catcggtctt
    17821 aaacgccccc gccgccgccc tgacgaaaaa caacggacgc aaaggcgaac gctccgccgg
    17881 actgtccgtc aacggcacgg gcgactaccg caacgaaacc ctgctcgcca acccccgcga
    17941 cgtttccttc ctgaccaacc tcatccaaac cgtcttctac ctgcgcggca tcgaagtcgt
    18001 accgcccgaa tacgccgaca ccgacgtatt cgtaaccgtc gacgtattcg gcaccgtccg
    18061 cagccgtacc gaactgcacc tctacaacgc cgaaaccctt aaagcccaaa ccaagctcga
    18121 atatttcgcc gtcgaccgcg acagccggaa actgctgatt gcccctaaaa ccgccgccta
    18181 cgaatcccaa taccaagaac aatacgccct ctggatggga ccttacagcg tcggcaaaac
    18241 cgtcaaagcc tcagaccgcc tgatggtcga tttctccgac atcaccgcct acggcgacac
    18301 aaccgcccaa aaccgtcccg acttcaaaca aaacaacggt aaaaaccccg atgtcggcaa
    18361 cgaagtcatc cgccgccgca aaggaggata aaccgtgaaa ccgctgcgca gactgacaaa
    18421 cctccttgcc gcctgcgccg tagcggcggc cgcactcata cagcccgccc tcgcggcgga
    18481 cttggcgcaa gacccgttca ttaccgataa cacccaacgg cagcactacg aacccggcgg
    18541 caaataccac ctcttcggcg acccgcgcgg cagcgtttcc gaccgcaccg gcaaaatcaa
    18601 cgtcatccaa gactataccc accagatggg caacctgctc atccaacagg cggcaatcca
    18661 aggcaatctt ggttacaccg tccgcttttc cggacacgga cacgaagaac acgccccctt
    18721 cgacaaccac gccgccgaca gcgcaagcga agaaaaaggc aacgttgacg acggctttac
    18781 cgtgtaccgg ctcaactggg aaggacacga acatcatccc gccgatgcct acgacggccc
    18841 gaagggcggc aattacccca aacctacggg cgcacgagac gaatacacct atcacgtcaa
    18901 cggcacagcc cgcagtatca aactcaatcc gaccgacacc cgcagcatcc ggcaacgcat
    18961 atccgacaac tacaacaacc tcggcagcaa tttctccgac cgcgccgatg aagccaacag
    19021 aaaaatgttc gagcacaatg ccaagctcga ccgccggggc aacagcatgg agtttgtcaa
    19081 cggcgtcgcc gccggcgcgc tcaacccctt tatcagcgcg ggcgaagcct tgggcatagg
    19141 cgacatactg tacggaacgg gttatgccat agacaaagcc gcgatgcgca acatcgcccc
    19201 cttgcccgcc gagggtaaat tcgccgtcat cggcggcttg ggcagcgcgg cgggctttga
    19261 aaagaatacg cgcgaagccg ttgaccggtg gatacaggaa aaccccaatg ccgccgaaac
    19321 cgtcgaagcc gtcttcaacg ttgccgcagc agccaaagtc gcgaagttgg caaaggcggc
    19381 aaagccgggg aaagctgcgg ttagcgggga tttctcaaaa tcctacacct gctctttcca
    19441 cggcagcacc ttggtcagaa cggcagacgg ctacaaagcc atcgcccata ttcaagccgg
    19501 agaccgcgtc ctttccaagg acgaggcaag cggagaaacg ggatacaaac ccgttaccgc
    19561 ccgatacggc aatccgtacc aagaaaccgt ttacattaaa gtttcagacg gcatcggcaa
    19621 cagccaaacc ctgatttcca accgcatcca tccgttttat tcggacggca aatggattaa
    19681 agcggaagat ttgaaagcgg gaagcaggct gtttgctgaa aacggtgcag agcagaccgt
    19741 tcaaagcgtt accgtcaaac cggaaccgct caaagcctac aatctgaccg tcgccgactg
    19801 gcatacttat ttcgtcaagg gtagtcaggc ggaaacggaa ggagtttggg ttcataatga
    19861 ttgtccgcct aaaccaaaac caaccaatca tgcccaacaa agaaaagaag aagctaaaaa
    19921 cgattctcat cgaagtgtgg gagattccaa tcgtgtcgtt cgcgaaggaa agcaatattt
    19981 agattccgac acaggaaacc atgtttatgt aaaaggagat aaagtggtta ttctaactcc
    20041 tgatggaaga caggtaactc aatttaagaa ctcgaaagcc aatacgtcaa aaagggtaaa
    20101 aaatgggaaa tggacaccaa aataatcaat cttccttaga aaaaagaatt ttttatctgg
    20161 agcattcggg gcagtatctg atgatttgcg ccctatcgga ttactcccaa aataaacata
    20221 ctgtcgttat ggcgaatttc ctctatccaa atgaaaaaat ggattggaga aatttagatg
    20281 atttgttcaa tgagttggtt ttagaggaat tacagtcttc atttatggat tggtatccaa
    20341 ctattgaaaa ggcaattagc catcatttag aagacttttc gtaacaagca taaatcaaaa
    20401 agtcgtctga aactgctttt aattttcaga cgacctttcc ccatatcggc ggattatgaa
    20461 tttacagcta tcgggaaatt gagttatctc tctgatagag aaaattccat acgcgaagcc
    20521 gttgaccggt ggatacagga aaatcccaat gccgccgaaa ccgtcgaagc cgtcttcaac
    20581 gttgccgccg ccaaagtcgc gaagctggca aaggcggcga aaccggggaa agctgcggtt
    20641 agcggggatt tttcgattag ctataaaaac ttttctactg tcaaaccaaa agtaattgcc
    20701 aaaggaacga ttaatggaaa gacttttaga gatgttaatc aaagtgcaaa aattggatcg
    20761 cctgattcac ctacattaat agcccaacga gttaatgcaa aaatccaagc cgacggtaaa
    20821 cctcgtccga atgcaaccgt tgctaattca catgctgaaa ttggtgttat ccagcaggct
    20881 tataatgcag gtgaaacgaa aggggcatct atgacaatga cagtttcagg aaaagatgtt
    20941 tgcggttatt gtaaaggaga tattgcagct gctgctcaag cttctggact gaaatcactg
    21001 actgttaatg cgacagataa tgtaacagga aaaaataaaa cttactattg gacgcctggt
    21061 atgaaatcaa ttaaggagag aaaatgaaaa catcaacaat tgtttttggt ggatttttta
    21121 ttacagataa tggagaacga atccaaatcc ctattttaga aaatcctaac attaaagaaa
    21181 taaataactt tttttctgta tcaaattttg aaaaaaaagc cggagtcctt gttttcagaa
    21241 ttattcctga gcctgaattt ggcaatactg aattaactat ctattttgaa aaaggatatt
    21301 atttaccaat aattcaaaca attttagaag atggagatat tgaagtaaaa aatcttaaaa
    21361 cagaaaatta tagtggaaat acaatggaaa ttttgggaga cgtctaccct attgagcata
    21421 tctctaaaaa tatttctata attcaagata taatttctga atttattatg aaaaataaac
    21481 caatcacaat aatgatttaa ttgaaaagtt caacaatcaa aaggtcgcct gaaatcgtgt
    21541 ttaattttca gacgacctat ttccttcatt tgaaacagga tattgaaaac taagttcttc
    21601 aaaaatccta cacctgccct ttccacggca gcaccttggt caaaacggca gacggctaca
    21661 aagccattgc ccgtatccgg gtcggcgacc acgtcttcgc caaggacgag gcaagcgggg
    21721 agacgggata caaacccgtt accgcccaat acggcaatcc gtatcgggaa accgtttaca
    21781 ttgaaatttc agacggcatc ggcaaca
//
LOCUS       DAWTCI010000026        20986 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWTCI010000026 DAWTCI010000000
VERSION     DAWTCI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20986
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.026"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(85..549)
                     /locus_tag="WM481_001003"
     CDS             complement(85..549)
                     /locus_tag="WM481_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080338.1"
                     /translation="MKRFILPVLLSATTALASPIVGTWHCIGTDENIHSDTKVKYLQD
                     GSFRGDAILKIDDDGNILAYRVVGEGKWRFANNALTQSQIKYSEVSRQHSPETLAWLE
                     KSEDGRLLESMMYTGLVAQMDKPGKDDVYQLDKSGKLVSEDGTSREACTKVE"
     gene            complement(626..3463)
                     /locus_tag="WM481_001004"
     CDS             complement(626..3463)
                     /locus_tag="WM481_001004"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFA9080339.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFRPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDGVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESMEEQGSMWHIRYPLADNPTEAVIVATTRP
                     ETLLGDAAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQPGFSLPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKPHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDNEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQAEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKAVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLMKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGHSFPDMWIIGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPETDGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGETGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEARQVAAL
                     PESGDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(3568..4827)
                     /locus_tag="WM481_001005"
     CDS             complement(3568..4827)
                     /locus_tag="WM481_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFA9080340.1"
                     /translation="MKVLVLGAGVAGVSSVWYLAEAGHEVTVIDRTEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKRLFKSHPPLLFRPDGGLYQIEWLWRMLQNCTATRYQ
                     INKERMVRISEYSREMFRRFEAQTDMNFEGRKKGTLQIFRQTEEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIVGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     YFNQTISRIDHNGLRIKAVETETGRFETDAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYETKLPEKRRETLAL
                     VVNDLFPEGGDLSQALSWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSPGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQKL"
     gene            complement(4906..6297)
                     /locus_tag="WM481_001006"
     CDS             complement(4906..6297)
                     /locus_tag="WM481_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9080341.1"
                     /translation="MQVFLDNLKAFFETVSSWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGAGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIVINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPELSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAALYRVGYVSSVMPGTVLSLDLVWLASDTFNGLMALPNLTALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(6559..6663)
                     /locus_tag="WM481_001007"
                     /pseudo
     CDS             complement(6559..6663)
                     /locus_tag="WM481_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(6760..7536)
                     /gene="lpxA"
                     /locus_tag="WM481_001008"
     CDS             complement(6760..7536)
                     /gene="lpxA"
                     /locus_tag="WM481_001008"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFA9080342.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVRIGANTEIGPHA
                     VINGHTTIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFQDFFAQSTRGIIR"
     gene            complement(7579..8259)
                     /locus_tag="WM481_001009"
     CDS             complement(7579..8259)
                     /locus_tag="WM481_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFA9080343.1"
                     /translation="MERYKNAVRKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CFRQEFKDRNRLYDSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDAAGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIYQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(8466..8915)
                     /gene="fabZ"
                     /locus_tag="WM481_001010"
     CDS             complement(8466..8915)
                     /gene="fabZ"
                     /locus_tag="WM481_001010"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFA9080344.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(8951..9994)
                     /gene="lpxD"
                     /locus_tag="WM481_001011"
     CDS             complement(8951..9994)
                     /gene="lpxD"
                     /locus_tag="WM481_001011"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFA9080345.1"
                     /translation="MIPATCTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAIIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRHVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTIVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHIAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDSKDTQ"
     gene            complement(10027..10527)
                     /locus_tag="WM481_001012"
     CDS             complement(10027..10527)
                     /locus_tag="WM481_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFA9080346.1"
                     /translation="MTRLTRAFAATLIGLCCTTGTHADTFQKIGFINTERIYLESKQA
                     RNIQKTLDGEFSARQDELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(10593..12971)
                     /gene="bamA"
                     /locus_tag="WM481_001013"
     CDS             complement(10593..12971)
                     /gene="bamA"
                     /locus_tag="WM481_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFA9080347.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSDRFDRQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTRQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAGTKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDIYGKAFDPRKASTSVKQYKT
                     TTAGGGVRMGIPVTEYDRVNFGLAAEHLTVNTYNKAPKRYADFIKQYGKTDGADGSFK
                     GLLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGRTYTAAENGNNKSVYSENAH
                     KSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(13028..14368)
                     /gene="rseP"
                     /locus_tag="WM481_001014"
     CDS             complement(13028..14368)
                     /gene="rseP"
                     /locus_tag="WM481_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFA9080348.1"
                     /translation="MQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARTGFQSGDKIQSVNGVSVQDWSSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTVEWIRGKPLGERVQNIGLRFGLALMMLMMAAAFFNDVTRLIG"
     gene            complement(14403..15638)
                     /locus_tag="WM481_001015"
     CDS             complement(14403..15638)
                     /locus_tag="WM481_001015"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFA9080349.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAAGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLSTFDSITPE
                     QAVKHPNWRMGRKISVDSATMANKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGKLDFGALSALTFQKPDFGRFPCLKF
                     AYETINAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(15643..16440)
                     /locus_tag="WM481_001016"
     CDS             complement(15643..16440)
                     /locus_tag="WM481_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFA9080350.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYA
                     RMAGLCKTETNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLLMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKALGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAIMSVLN"
     gene            complement(16443..17189)
                     /locus_tag="WM481_001017"
     CDS             complement(16443..17189)
                     /locus_tag="WM481_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFA9080351.1"
                     /translation="MKSSTQTILEHTAIPRHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIGQQRN"
     gene            complement(17245..17802)
                     /gene="frr"
                     /locus_tag="WM481_001018"
     CDS             complement(17245..17802)
                     /gene="frr"
                     /locus_tag="WM481_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFA9080352.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYIAEADKVLAAKEEDLMAV"
     gene            complement(17944..18936)
                     /locus_tag="WM481_001019"
     CDS             complement(17944..18936)
                     /locus_tag="WM481_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9080353.1"
                     /translation="MKIISLFSGCGGLDLGFEKAGFEIPAANEYDKTIWATFKANHPK
                     THLIEGDIRKIKEEDFPEEIDGIIGGPPCQSWSEAGALRGIDDARGQLFFDYIRILKS
                     KQPKFFLAENVSGMLANRHNGAVQNLLKMFDRCGYDVTLTMANAKDYGVAQERKRVFY
                     IGFRKDLEIKFSFPKGSTVEDKDKITLKDVIWDLQDTAVPSAPQNKTNPDAVNNNEYF
                     TGSFSPIFMSRNRVKAWDEQGFTVQASGRQCQLHPQAPKMEKHGANDYRFAAGKETLY
                     RRMTVREVARIQGFPDNFKFIYQNVNDAYKMIGNAVPVNLAYEIAAAIKKTLER"
     gene            complement(18941..19456)
                     /locus_tag="WM481_001020"
     CDS             complement(18941..19456)
                     /locus_tag="WM481_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9080354.1"
                     /translation="MTACLQKPAKMRKNGKRKTLSIIVGVVDKKKNLKHLAMVYGIDY
                     CADAECYLKIKNQIKEGIGNIGGIQFAETKELGRVNRIDPLNITYLRVRGMWGIENPW
                     FVFNYIYQRNMEKSFNFMAIINEDKWNSFNNTDKLLAIQDSKLAISDIKIKNPNNPAR
                     LRNAKLITYHL"
     gene            complement(19374..19778)
                     /locus_tag="WM481_001021"
     CDS             complement(19374..19778)
                     /locus_tag="WM481_001021"
                     /inference="COORDINATES: protein motif:HMM:NF021068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoPII family restriction endonuclease"
                     /protein_id="HFA9080355.1"
                     /translation="MNIIDAIINLANNPVVGVNSHSQSNNRANQAGDALEEYVKDLFS
                     GSFNLNETQRIARHAKVFSYLGNNSNPPDAMLRNGDAIEVKKIESKDSALALNSSHPK
                     SKLSVDDSMLTKACKDAEKWEEKDIIYNCRRR"
     gene            complement(19887..>20986)
                     /locus_tag="WM481_001022"
     CDS             complement(19887..>20986)
                     /locus_tag="WM481_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFA9080356.1"
                     /translation="GRWLNSYERYRHRRLIHAVRLGGAVLFATALARLLHLQHGEWIG
                     MTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTIGTA
                     SALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIGAAIAIAAAKL
                     LPLKSTLMWRFMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQINARMVKSRSH
                     LAATSGESRISPSMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRHFTL
                     LQTDLQQTAALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEISAL
                     VILLQRTRRKWLDAHERQHLRQSLLETREHG"
BASE COUNT         4256 a         5316 c         6010 g         5404 t
ORIGIN      
        1 acgataccgc cgaaaacggt attcggacgg cttgatttga gttttggtat ttttgcccga
       61 cggggtggaa aatacagttg cttttcactc cactttcgta catgcctcac gggaagttcc
      121 gtcttcggac actaatttgc ctgatttatc cagttgatac acatcatctt ttccgggttt
      181 gtccatctgc gcgaccaatc cggtgtacat catgctttcc aagagtcgtc catcctccga
      241 cttttctaac cacgccagcg tttcggggct gtgctgtcgt gaaacttcac tgtacttgat
      301 ttggctttga gttaaggcat tgttcgcaaa acgccattta ccttcaccga caacacggta
      361 tgccaagata ttgccgtcat catcgatttt gagtatagca tcgccacgga agctgccgtc
      421 ctgaagatat ttgacttttg tatcactgtg aatgttttca tcagtgccga tgcaatgcca
      481 tgtaccgaca atcggagaag ccaaggcggt tgttgcggat aaaagcaccg gcagaataaa
      541 gcgtttcatc tatttccttt tttaaaggag gccgtctgaa aaccccggtt tcaggttttc
      601 agacggcctt gtttattcaa tcaaatcagt ctttcaactt cgccaactga ttttgcactt
      661 ttgccatttt gtcttccaat tccgccaaat cggctttgtc tttttccacc agatgcgccg
      721 gggctttttc ggtgtagccg ggtttggaga gtttggcatt gagtttgtcc aaggcttttt
      781 gcagcttctc ggcttctttg ctcaaacggg cggtttcggc ggctttgtcg atttcgactt
      841 tcagcatcag gcgcgcgccg ttgcagacgg cgacgggcgc gtctccgctt tcgggcaggg
      901 cggcgacttg ccgtgcttcg gtcaggcggg tcatcatcgg caggtatttg aggtagtccg
      961 ccaagtcgtc cgcgctttcg acaaacagcg gggctttcac gttgggctgg atgcccgttt
     1021 cgccgcgcag gttgcggact gcgccgatca aatcctgcaa cacggtcatt tgcccgaatg
     1081 ccgtctgaac aatctcgccg ccgtcggttt cggggaaacg ggcgagcatg atgctgtcgg
     1141 cggttttggc atcgcacatg ggggcgacgg tttgccacag ttcttcggtg atgaacggga
     1201 taatcgggtg cagcaggcgc agggcggctt cgagtacgcg caataaggta tggcgtgtgg
     1261 cgcgctgacg gctggcgcag ccggtttgga gctgtacttt ggcgagttcc agataccagt
     1321 cgcaataatc gttccatacg aagctgtaca gggtttccgc cgccaaatca aagcggtagg
     1381 tttcgtaggc ttgcgtaacc tgctcgatgg tctgattcag acgacctatg atccacatat
     1441 cggggaagga atggccgcgc ggttcggcag cggttgcgcc gtagccgcag tcttggtttt
     1501 cggtgttcat caagacgaag ttggtggcgt tccagatttt gttgcagaag ttgcggtagc
     1561 cttcggcgcg tttgaagtcg aagttaaccg aacggcccaa gctggcgtag ctcgccatgg
     1621 tgaagcgcaa tgcgtccgcg cccatgctgg ggataccttc ggggaagagt tttttcgtgg
     1681 cttcttccac tttcggcgcg gtttcgggtt tgcgcaggcc ggtcgtacgt ttcatcagca
     1741 gcttgtccaa gccgatacca tcgatcaaat ccacagggtc gatgacgttg ccttcggatt
     1801 tggacatttt tttgccttcg tggtcgcgca cgatgccgtg gatgtacacg gctttaaacg
     1861 gtactttgcc ggtgaagtgg gtggtcatca taatcatgcg cgccacccag aagaagatga
     1921 tttcatatcc ggtaaccaag acgttggacg gcaggaaggc tttgagctcg tcggtttcag
     1981 acggccagcc gagcgtggag aacggcacga gcgcggagga gaaccatgtg tccaatacgt
     2041 cttcttcgcg ggtcaaacct gttttgccgg cttgtttttc ggcttcagcc tgattgcggg
     2101 cgacgtaaac attacctgct tcgtcgtacc acgcggggat ttgatgcccc caccacagtt
     2161 ggcgcgaaat acaccagtct tggatgttgt tcatccattg gttgtaggta ttgacccagt
     2221 tttcagggat aaagcgcact gcgccgctgt cgacggcttt tttggctttg tcggcaaggc
     2281 tcaagccttt gaattcgttg tcaggctcgc cgccgttcgg tgtggcggac atggcgacaa
     2341 accattggct ggtcagcatc ggctcaatca ctgaacctgt gcggtcgcct ttcggcgtca
     2401 tcagcgtgtg cggtttgatt tcgaccaaga agccttgctc ctgcaaatcg gcaaccattt
     2461 gtttgcgcgc ggcaaagcgg tccaagcctg cgtatttttc aggcagggaa aagcctggtt
     2521 gcgcttcgcc tttgaagttg aacacttcgg cgtttgccag cactttggct tctaagtcga
     2581 acacgttaat caggcgcgtg tcgtggcgtt tgccgacttc gtaatcgttg aaatcgtgcg
     2641 caggcgtgat tttcacgcaa ccggtaccga agtctttttc aacgtattcg tcggcgataa
     2701 ccggaatggt gcgaccggtc agcggcagga ttaattcttt gccgattaag tgggtgtaac
     2761 gttcatcttc tggattgacg gcaacggccg cgtcgcccag caacgtttca ggacgggtgg
     2821 tcgccacgat aacggcttcg gtgggattgt cggccagcgg atagcggatg tgccacatag
     2881 agccttgttc ttccatgctt tccacttcca aatccgatac cgccgtgcca agcacgggat
     2941 cccagttcac caagcgtttg ccgcggtaaa tcaagccctg ttcatacagg cgcacgaaca
     3001 cttcggtcac ggtttcggcg cgtacgccgt ccatcgtgaa atactcgcgc gtccagtcgg
     3061 cggagcagcc cacgcggcgc atttgctggg taatcgtgcc gccggaaact tctttccatt
     3121 cccacacttt ttccaagaat ttttcgcggc ccaagtcatg acgggacacg ttttgcgcgg
     3181 caagctgacg ctcgaccacg atttgcgtgg cgatgcccgc gtggtcggta ccgggaatcc
     3241 aggcggtgtt gcagcctttc atgcggtagt agcgggtcag gccgtccatg atggtttggt
     3301 tgaaggcatg acccatgtgc agcgtgccgg ttacgttggg cggcggcagt tggatggaaa
     3361 aagacggttt ggtcaaatcc atatcgggcc ggaaatagcc ttggctttcc cagttttgat
     3421 agtgtttgga ttcgatttcg gcgggggagt atttgtctaa catatgaagc cctgagaaat
     3481 aagatgattt gatggtttga attatagcgc aaatgccgtc tgaaacgctt tcggggtttc
     3541 agacggcctt aaatttaaat attgccttta cagtttttga tagcgcgaca ggctcaaatc
     3601 gtcgctgcgg atttcggtgt ctttgccgct cacgatatcg gcggtcaatt ttgccgaacc
     3661 cggcgacatg gtccagccca aagtaccgtg cccggtattc agaaacaggt tttcaaagcg
     3721 ggtgcgcccg attaacggcg tactgtcggg cgtcatcggc ctgaggccgc tccaggacaa
     3781 tgcttggctc aaatcgccgc cttccgggaa caagtcgttg acgaccaaag ccaaggtttc
     3841 gcggcgtttt tcgggcagtt tggtttcgta gccggacaat tccgccattc cgccgacgcg
     3901 gattctgttg tcaaaacgcg tgatggcgac tttgtagctt tcatctaaaa cagtggacac
     3961 cggtgcgccg tcggaatttg tgaccggcag ggtcaaggaa tagcctttga cgggataaat
     4021 gggcagattg agatccaact gcgccaacac agtcctgctg aagcagccga gcgcgcagac
     4081 aacggcatct gtttcaaacc gccctgtttc ggtttcaacg gctttgatgc gcagcccgtt
     4141 gtggtcgatg cggctgatgg tttggttgaa gtagaaccgt acgcccttct cttgacacaa
     4201 tttgtacagg ttttcggtaa agaggcggca gtcgccggtc gcatccgcag gcaggtgcag
     4261 accgccgaca attttggcgg taacgcgtgc cagcgcaggc tcgaattctg cgcattcttc
     4321 gggcttcaga cggcggtacg gcacgccgta gcgttccaaa acggcaatgt cttgttttgc
     4381 cgcttcgact tcttcggttt ggcggaaaat ctgcaacgtc ccttttttgc gtccctcaaa
     4441 attcatgtcg gtttgcgctt caaaacggcg gaacatttca cggctgtatt cggaaatcct
     4501 gaccatgcgc tctttattga tttgatagcg cgttgccgtg cagttttgca gcatccgcca
     4561 cagccattcg atttgataca ggccgccgtc agggcggaac agtaaaggcg gatggctttt
     4621 aaacagccgt ttcagtgctt tggtcggaat accgggtgca gcccaaggcg tggtatagcc
     4681 gtaagaaagc tggcctgcat tggcaaaact ggtttccatc gccacaccct cggtgcggtc
     4741 gatgaccgtt acttcatgtc cggcctctgc cagataccac acggaggata cgccggcaac
     4801 acccgcacct aaaacaagca ctttcatgtt tccccctccg gctttttcaa aacaggctta
     4861 atatgccgtg ccgtctgaat attcggattc agacggtctc ggatattaat gcggcaattc
     4921 gccgtttgtg attttttgtt tgaagtcgcg tgtttcattg acgatgactt tcgccatcaa
     4981 caaaagcgca gtcaggttgg gcaatgccat caagccgttg aatgtatccg aagccagcca
     5041 taccaaatca aggctcaaca cggtacccgg catgacggaa gaaacataac cgacgcggta
     5101 caaagcggca aacttctcgc cgaagacgta aaccgcgcat ttctcgccgt aataacacca
     5161 gccgagaatg gttgagtagg caaagaaaat caggccgatg gtcacaatcc agccgccgat
     5221 gccgggcagc attttttgga aagtgacggt tgtcagcgcc gcgccgctca attcaggttt
     5281 cacaaactcg ccgcctgcgc cgagcagacc catgaccaac acgataccgg taatcgagca
     5341 gacaacaatg gtgtccaaaa aagtaccggt catggaaacc aaagcctgac gaacggggtg
     5401 gtcggttttc gcggctgcgg cggcaatagg cgcagaaccc atacccgcct cattggagaa
     5461 cacgccgcgc gccacgccgt agcggatgac cgtaccgata gcgccgcctg ctacggcctg
     5521 cgcgctgaac gcatcggaga aaatcagctt gacggcaggc atcagtgcat cggaattaat
     5581 gacgataatg gaaagaccgc ccaacacata aaacaccgcc atagcaggca cgatgaaaga
     5641 agcggctttg gcgatgcctt taataccacc taaaacgaca acggcagtca gaacggtcaa
     5701 cgtaatgccg gtataggcag gttcgatacc gaagctggtt tgcacagcct gtgcaaccga
     5761 gttggactgc accgagctgc cgataccgaa ggaagcgaat gtgccgaaca gcgcaaatgc
     5821 aacggccatc catttccagt ttttgcccaa gcctttttcg atgtaataca tcgggccgcc
     5881 ggacatttcg cctttggaat tgttgacgcg gtatttcacc gccaacacgc cttcgccgta
     5941 tttggtggcc atgccgaaaa tagcggtcat ccacatccaa aataccgcgc ccgggccgcc
     6001 ggttaccact gcagtcgcca cgccggcgat gttacccgta ccgatggtgg cggacagcgc
     6061 ggtcatcaac gccgcaaaat gggaaatatc gccttcgtga cctgcgccgc cttcatactt
     6121 ctttgacggc acaaacgcct gtttcagcgc atagcccaac atagtgaact gcaagccttt
     6181 cagcataacg gtcagcacaa tgccggtgcc gaccagcagc atcaacataa cagatcccca
     6241 aacccagctg ctgacggttt cgaaaaaggc tttgagattg tctaaaaata cttgcatggc
     6301 tttctccttt gtctgtttta tttttaaaac accacttttg tagtgtccgg taatttcagc
     6361 acagaatatt caataagaca atatgttctt ttgaaaaata cttttggttt tttcaccgaa
     6421 gacaggccgg ttcaagctgc ggaaattgtt tgcaattatt taaaagcaga ggcggaggtc
     6481 acaatgaaat gtccgaatgg ggatgtggcg ggcggcagaa atcatcaatg ctgccgactg
     6541 ccatacttct gaaatctata gtggtttaaa tttaaaccgg tacagcgttg cctcgccttg
     6601 ccgtacttct tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaaaccact
     6661 atacaaaatg atgcatcgat caaacaacat accgctttaa aaaaaaccga tgccgtctga
     6721 aacgctttcg gggtttcaga cggcattgaa agggtgcggt cagcggatga tgccgcgcgt
     6781 cgattgtgcg aagaagtctt gaaatacggc aagctcggct tgggtttcgg cgcggcggag
     6841 aatgtccgcc ttggcttctt caaacggaat gccgcgatgg tagagggttt tgtatacgtc
     6901 tttgacggcg gaaatctgtt ctgcggtaaa accgttgcgg cgcatacctt cgctgtttag
     6961 gccggccggt tcggcgcggt agcccgatgc cataaagtag ggcggcacgt ctttgtgtac
     7021 gcctgcggcg aaggcggtca tggcgtagtc gccgatgcgg cagaattgga agaccagcgt
     7081 gtagccgccc aaaacgacgt agtcgccgac ggtaacgtgt ccggcaagcg aggcgttgtt
     7141 ggcgaaaatg gtgtggttgc cgaccacgca gtcgtgcgcg aggtggcagt acgccataat
     7201 ccagttgtcg tcgccgatac gggtttcgcc gatgccggtt accgtgccta aattaaaggt
     7261 ggtgaattcg cggatggtgt tgccgttgcc gataatcagc ttggtcggct cgtcgcggta
     7321 ttttttgtcc tgcggaattt cgccgaggct ggcaaattga aaaatgcggt tgttttcgcc
     7381 gatggtggtg tggccgttga tgacggcgtg cggaccgatt tctgtattcg caccgattcg
     7441 gacgttgggg ccgataacgg tgtacgcgcc gactttgacg ccggagtcga gttcggcttt
     7501 ggggtcgatg acggcggtcg ggtggatgag ggtcatgttt tgaatactcc gctgcttgtt
     7561 aagacaaata atagaaaatc acagtttgtc gatttcatcg cagaccgcaa tcacttgatc
     7621 gctaaaatgg ctaaatactg aagcagtatc tacatccatg taattaagca gttcaaaaat
     7681 agattggtaa atatttctag cgtagccggt atcttttatt gcacctttaa aacaaatcgg
     7741 ttcgtgatgt gccaaacgat tacggaaatt gttaatattg gaaagttctc gaaaaatcca
     7801 tttttggtta tattgcacac ttggtgtaga tttgggcttt tttggaaaaa ctttcatcaa
     7861 tacttttcct gcagcatcaa attgagcatc tttccctccc gcaaacagat accgccaaaa
     7921 accgaatcct agtcctgcaa ccaattggtt gtggtcaaac tttcctccgc tattattttg
     7981 tattttagat aatgcaacct tgattaattc ggcagattct ttcgtaccat ttcttaaaaa
     8041 gcaaccttga tatttcaatg caggattcgt ttgtggttgt atgctatcat ataaccggtt
     8101 tctgtcttta aactcctgcc gaaagcaaat atcaattttg tttcttaaaa cgatctcaaa
     8161 tatagaaaca acatgaaaaa gttctcttga taaagataaa ttcaataaat acaattcagc
     8221 tgctttgtct ttcctaacgg catttttata ccgttccatt ctgaaatcag aaaaaatact
     8281 acagcattcc ttataattca cgctaaccct tgattcttta tttttaaatt gacgtttcaa
     8341 actatatttg atatagttag ataaaactaa tccccaccgt gtccctgggt ttgaataaac
     8401 ctaaaccggt gggttttctt tttattaggt cgtctgaaac tttcagacga cctttttccg
     8461 aacgctcaaa ccacgcgttt ggcgcacata atcacggctt cgacggcgac ttgtccgtcc
     8521 actttggcaa cggcgttgaa tttgccgatg ccgcgccggc tggtcaggag ttcgacttca
     8581 aagacgagtt ggtcgccggg gatgacttgg cgtttgaaac gggcttcgtc tatgccggcg
     8641 aagaagaaaa attcgttttc cttgcgcccg ccttcgctca aaatcgccaa cgtgccgcac
     8701 gcctgcgcca tcgcttcgat gatgagtacg ccgggcataa cgggcaggtc ggggaaatgg
     8761 ccttggaatt ggggttcgtt tatggttacg tttttgatgg cggtcagggt tttcatcggc
     8821 tcgaaggcgg taatgcggtc gagctggaga aacgggtagc ggtgggggat gagtttttgg
     8881 atgtctttgg cttcgatggg gagttgtacg tccatgtctg tcgtattcct tgaataaagt
     8941 cggtttggat ttattgcgtg tctttgctgt ctgaaagctg ctgttccagc gttttgaggc
     9001 gtttgttcat ttcgcttaag cggtggatgt aaacggcgtt gcgcgcccat tctttatggg
     9061 tggacatcgg gaagatgccg gcgatgtgtt tgccgctttc ggtaatgctg tgggtaacgg
     9121 acgtgccgcc gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtgccgactc
     9181 cgccgccgat gatgcagtag ctgcctatgg ttacactgcc ggagatgccc gttttggcgg
     9241 cgatgacggt gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt
     9301 tggtgccgtt gccgacaatg gtgtcgctca tcgcgccccg gtcgatgttg gtgttcgagc
     9361 cgatttctac gtcgtcgccc agcgttaccg cgccggtttg cgggattttg aaccacgaat
     9421 cgccggcgaa ggcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga
     9481 cgtgtctgcc gagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt
     9541 cgcccagttt gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga
     9601 gcacggtgtt cgcgccgatg taggcgttcg cgccgatttc acagcttgcg ggaacggtcg
     9661 cgcccggttc gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa
     9721 acaggcgcgc gactttggcg aaatagagat agggatcgtc ggcgacaatc aggttgcgcc
     9781 cttcaaatcc gtctgctgct ttggcggaaa cgatgatcgc gcccgcgctg ctgtcgtgga
     9841 cttcggcttt gtatttcgga ttggcgagga agctgatgtg ttccgcctgc gcgtctgcga
     9901 gcgggcgcac ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg
     9961 tgatttggga cagggtgcag gtggccggaa tcatggtttt cctgtttggt atgccgtctg
    10021 aaagggttag cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt
    10081 gacgtaaatc acgtcctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat
    10141 gacgcggttg gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc
    10201 ttcttcaaac tgcgcctgtt ttttgcggaa cgcttcgacc agcccgcgcc atttttcttc
    10261 ggcttgcgcc ttttttgcgt ccttaagttt gccgccggcg agctgccttt ccaaatccaa
    10321 gccttcgcgt tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc cgtccagcgt
    10381 tttttggatg ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc
    10441 gattttttgg aaggtgtcgg cgtgcgtgcc tgtggtgcag cataaaccga tcagggtggc
    10501 ggcaaacgcg cgggtcaaac gggtcatggt aaaactcctt cggatgttgc cgcgaaatgc
    10561 cgtctgaagg gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ctggaattgg
    10621 aagcgttgga tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc
    10681 gggcccaaag gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta
    10741 aaggtggatt tatgcgcgtt ttccgagtaa accgatttgt tgttaccgtt ttcggcggcg
    10801 gtataggttc tgccgtccca cacgctgcct gcgtcggcaa acaggctcag gcggacggtg
    10861 cgtgcgtctt tcgcaccggg catcgggaag agcagctcgg cggagacgtt ggcttttttg
    10921 ttgccgccgt agctgatttt ttcgccgtat tcgtcataca ctttcgggcc gagcgtgccg
    10981 ctttcgtagc cgcgcaccga acccaggccg ccgccgtaga agttttcaaa gaaggggatt
    11041 tctttggttc tgccgtagcc gcccgcaatg ccgacttcgc cgccgagcat cagcgtgaag
    11101 gttttgctta aggggaagaa ccaggtttgg ttgtgggtgg cggagtagta ttgcagtttg
    11161 ctgccgggca gggcgatttc ggcatttacg ccggtcaggt agccgcgcgt cggccataag
    11221 gcgctgtcgg tcttgttgcg cccccagccg acagtgcctt tgtacagcag gcctttgaag
    11281 ctgccgtctg cgccgtcggt tttgccgtat tgtttgataa agtcggcata gcgtttgggt
    11341 gctttgttgt aggtgttgac ggtcaggtgt tccgccgcca gcccgaaatt gacgcggtcg
    11401 tattcggtaa cggggatacc catccttacg ccgccgccgg cggtggtggt tttatattgt
    11461 ttgacgctgg tcgatgcttt gcgcgggtcg aaggcttttc cgtaaatatc gtagcccagg
    11521 ctgaccccgt ctgccgtgaa gtacgggtcg gtaaacgaca gcgagccgtt gagcgtggtt
    11581 ttgcttcgcg aggcgcgcag ggcggccgac ttgcccgtac cgaacaggtt gtcctgcgat
    11641 acgccggcgg acatgaccaa gccggtatcc tgaacccagc ccgcgctcaa gtcgagcgag
    11701 ccggtggagc gttcggtcag gctcatgttc aaatcgactt tgtcgggcgt gccggcaagc
    11761 gggacggcat caaactgtac gttgtcgaag tagcccaaaa gctcgacgcg ctctttggaa
    11821 cgttgcagct tggaggtgtc gtaaggcgcg gattccattt ggcgcaattc gcggcggacg
    11881 acttcgtcgc gggttttgtt gttgccggtg atgtggattt cgttgacgta gatttttctg
    11941 cccggttcga tgtgcaggac gaaatcgacg gttttggttc cggcgttcgg cagcggctgt
    12001 acgctgattt cgctgtatgc gtagcctgcc gagcccatgc ggttctgaat ctcacccaaa
    12061 acggcggtca tctgctggcg ttcgtaccat ttgccgggct tcatggtcag cagtttttcc
    12121 agttcggcct tggggacttc gttggtgtcg ccttcaatcg acactttgcc ccagcggaaa
    12181 cgtccgcctt cgtggacggt gattttgatg gtctgcctgg ttttgtcttc gttggtttgg
    12241 atgtcggtat cgaggatacg gaaatcgaag tagccgttgt tctggtagaa gtcggttact
    12301 ttttccatgt cttgggcgaa tttctggcgg tcgaaccggt cgcttcgtgt cagccatgtc
    12361 caaatgccgc cttcggtcag cgacatctgc cgcatcagtt tgcggtcgga atagacttgg
    12421 ttgccttcaa attcgatgtc ggtgattttg gcggatttgc cctcgtcaat cgtgatgtcg
    12481 atgtcgacgc ggttgcgggc gagtttggtt actttgggcg tgatttggat attgagtttg
    12541 ccgcgcccga ggtattcttc tttcaggccg gcgactgcct ggttgagtgt cgcctgatta
    12601 aagtattgcg actgcgccag cccgaacgat tcgaggtttt tcttgatggc gtcgttttgc
    12661 agcattttgg cgccggtgat gttgagcgag ccgatggtgg ggcgttcgat aacggtcagc
    12721 agaagctgcc cgtccgcagt ttcgactcgt acgtcgtcaa agaaaccggt ggcgtacagg
    12781 cttttgatga tggcactgcc gtgtgtgtcg ttgtaggtgt cgccgacttt gacgggcagg
    12841 tagttgaata cggtgctcgg ctcggtacgc tgcaagcctt cgacacggat gtcttggatg
    12901 gtgaagtcgg caaatgccaa aggcgatatg cccaacatca tcagtgcgga ggcaatctgt
    12961 ttcagtttca tcgtaagttc cttgtggtgc ggaatgcggt ttcagactgc attccgaaac
    13021 gtaaaatcta accgatcagc cgggtaacgt cgttgaagaa ggcggccgcc atcatcagca
    13081 tcatcagggc gagcccgaag cgcaaaccga tgttttggac acgttcgccc aaaggtttgc
    13141 cgcgtatcca ttcgacagta taaaacacga ggtgcccgcc gtccaaaacg gggacgggca
    13201 gcaggttcag cacgccgagg ctgatgctga ccaacgctaa aaattccaaa taactttgca
    13261 agccgagttc ggcggactgt ccggcaatgt cggcaatggt cagcggcccg gaaatatggc
    13321 tgacagaggc gttgccgctg attagtttgc cgaaaaattt gagggttgtc cacgagtggg
    13381 aaacggtttt ttcccagccc atgccgaatg cgcggacaac agacggacgg tagctgcggc
    13441 ggatttgcgc gtcccacgcc ctgtccggct gcggacggag gccgacgcgc ccgatcaggg
    13501 tgtggtcggg ctgttcgaca gtatcggggc ggatgtcggc ggtatgggtt tgtccggcgc
    13561 gttcgtaggt cagggtgatt tttttgcccg ggctttggcg ggtcaggttt gcccattcct
    13621 gccatgaggc gatgggtttg ccgtcggcgg cagtcagcct gtcgcccggt ttcaggcctg
    13681 ctttttcggc ggggctgcct ttttccacgc cgccggcaac ggttgtgatt ttaaagggca
    13741 tcagtccgat gtagccttgg ttttttgcga ttttaccggc ttccggcgtg cctgcggcat
    13801 cgatggtgcg gacggtttgc gcgcccgatg ccgtctgaac gccgacggcg actttgccgg
    13861 cttcgaggtt gaggacgatt tcggtttgcg cgctgctcca gtcttggacg gaaacgccgt
    13921 tgacggattg tattttgtcg ccgctttgga agccggtgcg ggcggcaatg gtgtcgggtt
    13981 cgactgtgcc gacatagggc cgcagttcgg ttacgccgaa ggaaaagctc agtccgtaca
    14041 gcaaaaccgc cagtgcgagg ttggtcagcg gaccggcggc gacgatggcg atgcgcttgg
    14101 cggggtgttg tttgtcaaaa gcgtagggta aatcggcttc tgatacttcg ccttcgcgcg
    14161 tatcgaccat tttgacgtag ccgcccaacg gaatcggggc gaggcaccat tcggtgtcgc
    14221 cgcgctttcg ggtgaaaaac ggtttgccga agccgacgga aaaacgcaca accttgacgc
    14281 cgcacaacct ggcgacgatg tagtgtccga attcgtgcag gctgaccaaa atcaggatgg
    14341 cgaagataaa agctagaagg gtttgcaaat ggttttcctt tgataacggt gttcggacgg
    14401 tatcagcgca gtgtgccgat aaatgcccgc gcttgtgcgc gtgtccgggc atcttgcgcc
    14461 aacagtcctt ctatatcgcc catgccgttt gaaaagtctt gtgcaagaca gtgggcgacg
    14521 gttttggcaa tgtcggtaaa cttaatctgt ccgtccaaaa aggcggcgac ggcggtttcg
    14581 ttggcggcgt tcaatacgca gggcgcggct ccgcctgcgt ttatggtttc ataggcgaac
    14641 ttcaggcagg ggaagcggcc gaagtcgggc ttttggaagg tcagcgcgga caatgcgccg
    14701 aaatcgagtt tgccgacacc cgaatcgatg cgctcgggca agcccaaaca ataggcgatg
    14761 ggcgttcgca tatcgggatt gcccagttgc gccagcacgg agccgtcgcg gtagcgcacc
    14821 atactgtgta tcacggattg gggatggatg acgacttcga gtttgtcggg cggacagttg
    14881 aacagccaat gcgcttcaat cagttccaag cccttgtttg ccatagtggc tgaatcgacg
    14941 gagattttgc gccccatacg ccaattgggg tgtttgaccg cctgctcggg cgtaatgctg
    15001 tcgaacgtgc ttaaatcggt tgttaaaaac gggccgccgg aagcggtcag gataatcgaa
    15061 tcgatgccgt gttcgttcag acggtctgtg taatcgcgcg gcaaaacttg gaaaatggcg
    15121 ttgtgttcgc tgtcgacggg caacactgcc gcgccgtttg cgcgggcggt ttccataaac
    15181 aacgcgccgg aaaccaccag cgtttctttg tttgccagat aaatggtttt gcctttttgc
    15241 gccgctgcga gcgcggaagg cagccccgcc gccccgacga tggcgcacat gacgccgctg
    15301 acttcgtcgg cagacgcaac gtcaaccaat gcctgcgcgc cgtgtaaaac ctgagtcgcc
    15361 gtgccgtcgc gtttcaacag ggcttcaagc cgggcggcgt gttcggcatc ggcaacgacg
    15421 gcatattcgg ggcggaacgt ttgacattga gccgccagtt tctcgacctg cttatgccct
    15481 gccagcgcga atacgcggaa tttttcggga tggcgggaga caacgtccag cgtgctttcg
    15541 cctatgctgc cggtactgcc taatatggtc aggacttgtg gtgtcataat gaggataact
    15601 ttataccgga tgccgtctga agcgttttca gacggcatag aatcaattta aaaccgacat
    15661 tatcgccgca tagacgctga taacggcaat caggctgtcg gtacggtcga acacgccgcc
    15721 gtgtccgggc agcaggttgc tgctgtcttt gatgcctgcc gcgcgcttga gccagctttc
    15781 caacaggtcg ccgcatacgc tgacaacggt caacaccaaa ccgattaaca cggtatcgaa
    15841 ccagcctgta tcgaatgcca gccagccggc acttcgtacg gcagtcatgt acactgccac
    15901 gcaaaccgca ccgccgattg cgccttccca gctcttgccg gggctgattg ccggtgcgat
    15961 tttgtgtttg ccgagcgcct tgccgctgaa atacgcgcaa acgtcggcaa cccagaccaa
    16021 acccatcacg gcgagcagcg gcagggcatc atcgggatgc gggcgcaggg atacgagcgc
    16081 gaaccaaaac ggcatgagca aaagccagcc gacggcataa acctgccaac cgccgttgag
    16141 cctccatttg aatctcaacc acaaaggcat aacggcgagc caaaatgcca aaacaacata
    16201 ccaaaccaaa ttaggcagca tccagccgcc cgcataggca actacgccga aaaccaaggt
    16261 tgcggcgagg taatggttgg tttcggtttt gcacaaaccg gccatacggg catactccca
    16321 caaggcggtc agggcaatca gcccgcaaaa tgcagcccac agccattgcg gcgcgtaaaa
    16381 cagcatgccc agcatcagcg gcagcagcca catagcggtt attacccgtt gtttcagcat
    16441 attcagttcc tttgctgtcc gataggcagt tgctcggaag tgcgtccgaa ccgccgttcg
    16501 cgtttttgga acgaagcgac ggcggcatct aaggcggttt cgtcgaaatc gggccacagg
    16561 atgtcggtga aatacagttc tgcatacgcc atctgccaga gcaggaaatt gctgatgcgc
    16621 gtttctccgc ccgtgcggat gaacaaatcc ggttccggcg cgtcgcccag catcaagtgt
    16681 ttggccagcg tgtcttccgt aatctcggat acgccttcgg caatcagttt gtttgccgcc
    16741 tgcaaaatat cccagcggcc gccgtaatcc gcagcaatgc tcagggtcag accggtatta
    16801 tttgccgtca aggcttccgc ctcttcgata ccttgcagaa tctgccgatt aaagcgttcg
    16861 cggctgccca atattttcag gcgcatattg ttttcgtgca ggcggcgtac ctgtttttgc
    16921 aaagcctgta aaaacagccc catcaggaac gaaacttcgt cttcggggcg acgccagttt
    16981 tcggttgaaa aggcaaacac ggtcagatat tgcacgccca gtttggcgca atgcttcacc
    17041 atattttcca acgcgtccaa gccgcgtttg tgtcccatta tacgcgggag aaaacgtttt
    17101 ttcgcccaac ggccgttgcc gtccataatt acggcgatgt gcctcgggat ggcggtgtgt
    17161 tccaaaatgg tctgcgtgct gctcttcata tctgcctttc gcggttcggc gttcaaatgc
    17221 cgtctgaacg ccgcgccgtg agatttaaac cgccatcagg tcttcttctt tggcagccaa
    17281 aaccttatcg gcttcggcaa tgtacttgtc ggtcagtttt tgaaccgcct cttcgccgcg
    17341 acgtgcctcg tcttcggaaa tttctttgtc tttgaggagt tttttgatgt ggtcgttggc
    17401 atcgcggcgc acgttgcgga tagagacgcg tccttcttcc gcctcgccgc gcacgacttt
    17461 aatcaggtct ttgcggcgtt cctcggtcag catgggcatc ggcacgcgga tcaaatcgcc
    17521 gacagctgcc gggttcagtc ccaagtttga atcgcggatg gctttctcga ctttggccgc
    17581 catattgccc tcaaacggtt tcacgccgat ggtgcgcgcg tccaaaaggg ttacgttggc
    17641 aacctggctg acggggacca tgctgcccca gtattcgact tccacttggt cgagcaggcc
    17701 ggtatgcgcg cggccggtac gcactttcgc cagattttct ttcagtactt cgaccgaacg
    17761 ctgcatcttg ccttcggctg ttttttgaat atcgttgatc atattgttct ttcggtaaga
    17821 taaggtaggc gggcagccgt tcgaacgcgc tccaagccgt tcagacggca taaagaccgt
    17881 taactgcgaa tagtaccgtt attcgggcat gacgacaagg cagatgcctg taggaagggt
    17941 gtttcacctt tctagggttt ttttaattgc cgctgcaatt tcgtaggcaa ggttgacggg
    18001 gacggcgttg ccaatcattt tgtatgcgtc gttgacattt tgatagatga atttgaagtt
    18061 gtcgggaaag ccttggattc ttgcaacttc gcgtaccgtc atcctccgat atagtgtttc
    18121 tttgccggca gcaaaacggt agtcgtttgc cccgtgcttt tccatctttg gggcttgcgg
    18181 gtgcagttgg cactgcctgc ctgaagcttg gacggtaaaa ccctgttcat cccacgcttt
    18241 aactcggttc cggctcataa aaatagggga aaaactgccg gtaaaatatt cgttgttgtt
    18301 gactgcgtcg gggttggtct tgttttgcgg ggcggaaggt acggctgtgt cctgcaaatc
    18361 ccaaataacg tctttcaatg taatcttgtc tttgtcttcg accgtcgaac cttttggaaa
    18421 agaaaatttt atttccaagt ctttacggaa accgatgtag aagacccttt tgcgttcctg
    18481 tgctacaccg tagtctttgg cgttggccat agtcaaggtt acgtcgtatc cgcatctgtc
    18541 aaacattttc agcaggtttt gtacggctcc gttgtggcgg tttgccagca ttccgctgac
    18601 gttttccgct aaaaagaatt ttggctgttt gcttttcaaa atacggatgt agtcgaaaaa
    18661 caactgtccg cgcgcatcgt cgatgccgcg caaagctccc gcttcagacc aagactggca
    18721 gggtgggccg ccgataatcc cgtcgatttc ttcagggaaa tcttcttctt taatcttgcg
    18781 tatatcgcct tctatcaaat gggtctttgg atggtttgcc ttgaaggttg cccaaatggt
    18841 tttatcgtat tcgttggcgg cggggatttc aaatcccgct ttttcgaaac ccaaatccaa
    18901 accgccgcaa ccgctaaaca aactaatgat tttcatagga ttataagtgg taggtgatga
    18961 gttttgcatt tcttaatcgc gccgggttgt tgggattttt aattttaatg tcggaaatgg
    19021 ccagtttgga atcctgtatg gcaagcagct tgtctgtatt gttaaaactg ttccatttgt
    19081 cttcgttgat aatggccatg aaattgaaac ttttttccat attccgttgg taaatatagt
    19141 tgaagacaaa ccatggattt tcgattcccc acataccccg tactctcaga taagttatgt
    19201 tgagcggatc aatgcggttg accctgccca gctctttggt ttctgcaaac tgtatgccgc
    19261 cgatgttgcc gattccttcc ttaatttgat tttttatctt caaatagcat tcggcatcgg
    19321 cgcagtagtc gattccgtaa accattgcca agtgtttgag gtttttcttt ttgtcaacga
    19381 cgccgacaat tatagataat gtctttctct tcccattttt ccgcatcttt gcaggctttt
    19441 gtaagcatgc tgtcatcaac agagagcttt gattttgggt ggctgctatt cagcgccagt
    19501 gcggaatctt tactttcaat tttcttaact tcaatggcat ccccgtttcg caacatggca
    19561 tcaggagggt tggagttgtt gcccaaatat gagaatactt ttgcgtgtcg ggctatgcgt
    19621 tgggtttcat ttaggttgaa actgccggaa aacaagtctt ttacatattc ttccaaagca
    19681 tcacctgcct gattggcacg attgttgctt tgagaatggg agttgacccc gacaacaggg
    19741 ttgttagcca aattgatgat ggcatcgata atgttcatgg tttttccttg gggttttgcg
    19801 gcagattata gcaaaaccgt cagggtgttt atcggaccga tgccgtctga agcggcaggc
    19861 gtttcagacg gcatcgtgtc cggccgtcag ccgtgttccc gtgtttcaag caggctttgg
    19921 cgcaggtgtt ggcgttcgtg ggcatccagc catttgcggc gggtgcgttg cagcaggatg
    19981 acgagggcgg aaatttcctg acgcatattg gtgctgagcc agaggaagcc ctgccattgg
    20041 tagtggaggt gttcggcgag ggcttccagt tcggggttga tggcggtgtc gatgcggatg
    20101 cggcgggcgt gtctgccgtt gatgagggcg gcggtttgtt gcaggtcggt ttggagcagt
    20161 gtgaagtggc ggtcgagcag ccggatttcg ctgccgttga gtttgggaga ttgcagcttg
    20221 gcggcggtgg tcaggagcag ctcggtggtg ttgacgattt tgcggtgggc gtgctgcatg
    20281 gcttccatca tggaggggct gatgcggctt tcgcccgatg tggcggcgag gtggctgcgg
    20341 cttttgacca tgcgtgcgtt gatttggcgc attttgacca tattctgctc caaacgttcg
    20401 cgcgtcatac gcctgccgtt gctgatttcg gcaatcattt tgctgcagtc ggccaggttg
    20461 tcggcaagca tgaaacgcca catcagtgtg gatttcagcg gcagcagttt ggcggcggca
    20521 atggcgatgg cggcgccgat gaggacgttc atcgcgcgca tcaggccgct gtcgagccat
    20581 tcgctgccgt tgtcgccgat gagcatgcac atcgtcagcc ccgccagcat agggacgtag
    20641 ccgtttttgc cgaccgccgc ccagccggcc agtgcgcttg ccgtgccgat ggtcaggtag
    20701 aagaggaggt tgccgtggaa ataatgctgg ttcagccata aaacgcccaa acccgcgccc
    20761 agcccgatga ccgtaccgag catacgttcc accgccttgg agtaaatcgc gccttggaac
    20821 tggagcatgc cgaggacgac gaagacggtc atccctatcc attcgccgtg ttggaggtgg
    20881 agtagccggg cgagtgcggt ggcgaacagg acggctccgc cgagccgcac ggcatgtatg
    20941 aggcggcggt ggcggtagcg ttcgtaggag ttgagccagc ggccgg
//
LOCUS       DAWTCI010000027        20619 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWTCI010000027 DAWTCI010000000
VERSION     DAWTCI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.027"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..162)
                     /locus_tag="WM481_001023"
     CDS             complement(<1..162)
                     /locus_tag="WM481_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080357.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRSELA"
     gene            328..1251
                     /locus_tag="WM481_001024"
     CDS             328..1251
                     /locus_tag="WM481_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9080358.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFLG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETGCESEPRIPFEEKILFVDE
                     HLIVVDKPHFLPVIPGGRFLRETLLTRLRLRPELQHLNVEDITPIHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTKTDLPYPLDVVSRLVRGEKFFTTQEAEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(1330..1971)
                     /locus_tag="WM481_001025"
     CDS             complement(1330..1971)
                     /locus_tag="WM481_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9080359.1"
                     /translation="MLKPETIHIPGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQVRIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIEKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(2026..2817)
                     /locus_tag="WM481_001026"
     CDS             complement(2026..2817)
                     /locus_tag="WM481_001026"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFA9080360.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     RPSELVLSYSRAMMGWLLFAERLPQHITQIGLGGGSFARWTDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGGKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLKLPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            2983..3058
                     /locus_tag="WM481_001027"
     tRNA            2983..3058
                     /locus_tag="WM481_001027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3016..3018,aa:Thr,seq:tgt)
     gene            3200..3991
                     /gene="panB"
                     /locus_tag="WM481_001028"
     CDS             3200..3991
                     /gene="panB"
                     /locus_tag="WM481_001028"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFA9080361.1"
                     /translation="MITVNTLQKMKAAGEKIVMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDEAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMRGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            4114..4950
                     /locus_tag="WM481_001029"
     CDS             4114..4950
                     /locus_tag="WM481_001029"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFA9080362.1"
                     /translation="MQIIHTIRELRTWRENIGKVAFVPTMGNLHEGHLALVREARKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLAAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPHLQNELCGKFRPGHFRGVATVVSKLFNIVLPDVACFGKKDYQQLAVIKGLTED
                     LNFDIEIVPVDTGRAADGLALSSRNRYLSVGERAEAPRLYRELQAVAESLKQGGLDYA
                     GLERQAADHLTAAGWLVDYVEIRRADTLEMARAGDKKLVVLAAARLGTTRLIDNVEVG
                     LP"
     gene            5277..7115
                     /locus_tag="WM481_001030"
     CDS             5277..7115
                     /locus_tag="WM481_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080363.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADVELPKEVGKVLRKHRR
                     YSEEEIKNERARLAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEV
                     LAQSDDVQKRRIFLLLVQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQG
                     REKEKAIEALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLRKPDDAYARLNVLLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALIGLNNIIAKLSAAGST
                     EPLAEALAQRSIIYEQFGKRGKMIADLETALKLTPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRYGIALPEPSRKPRK"
     gene            7255..7836
                     /locus_tag="WM481_001031"
     CDS             7255..7836
                     /locus_tag="WM481_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFA9080364.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELGRQLVGFKLPIQYLHIWAEGRRVAGAPYRIRSDGILEQYGWTIGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            7846..8691
                     /gene="ispE"
                     /locus_tag="WM481_001032"
     CDS             7846..8691
                     /gene="ispE"
                     /locus_tag="WM481_001032"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFA9080365.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNLAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQWQLIDLGAALGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTYEGLTRDSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSKYGSAMMTGSGACIFAAFQARNSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            8699..8774
                     /locus_tag="WM481_001033"
     tRNA            8699..8774
                     /locus_tag="WM481_001033"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8732..8734,aa:Gln,seq:ttg)
     gene            8787..8862
                     /locus_tag="WM481_001034"
     tRNA            8787..8862
                     /locus_tag="WM481_001034"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8820..8822,aa:Gln,seq:ttg)
     gene            9030..10013
                     /locus_tag="WM481_001035"
     CDS             9030..10013
                     /locus_tag="WM481_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFA9080366.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGKTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            10080..10652
                     /locus_tag="WM481_001036"
     CDS             10080..10652
                     /locus_tag="WM481_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFA9080367.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTAVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(10778..11947)
                     /locus_tag="WM481_001037"
     CDS             complement(10778..11947)
                     /locus_tag="WM481_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFA9080368.1"
                     /translation="MTAHKILPVLLPIILGVSHATAASPAPNRPTVHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNTNTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALCANDAALTLADRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLGREGQVSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFENIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNRALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGHTIAEKEIVALENVEKRSRWQRLWTRLTGQ"
     gene            12096..13622
                     /gene="ilvA"
                     /locus_tag="WM481_001038"
     CDS             12096..13622
                     /gene="ilvA"
                     /locus_tag="WM481_001038"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFA9080369.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNGTFRLCKELLDEIITVDTDTVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVGHERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(13678..14754)
                     /locus_tag="WM481_001039"
     CDS             complement(13678..14754)
                     /locus_tag="WM481_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFA9080370.1"
                     /translation="MSITIQNLNKKRFGNFHALKNINLNVPAGKLVSLLGPSGCGKTT
                     LLRIIAGLENADGGQILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTV
                     LPKPERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLL
                     LDEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQ
                     TGSAEAIYRKPENAFVTEFLGEADAFEGRIEKGIWHYNGFAWKLDAQYKWQEQTATGY
                     IRPHEWQIAAEHETPMIRAEIEKIHAVGALTHVLVKHGKQDVHITLAGSDAARYPIAE
                     GKKLNLIPKQVYVFSQNELIEYSI"
     gene            complement(14792..14899)
                     /locus_tag="WM481_001040"
                     /pseudo
     CDS             complement(14792..14899)
                     /locus_tag="WM481_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(14895..15716)
                     /gene="cysW"
                     /locus_tag="WM481_001041"
     CDS             complement(14895..15716)
                     /gene="cysW"
                     /locus_tag="WM481_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFA9080371.1"
                     /translation="MKPYSANPNLTEPRRLRMLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAVKLTLITALIAVPVNAVLGVAMAWLLTRFDFLGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGSRLEAQGIQIIFAIPGIVLTALFVTFPFAA
                     REIIPLMQTQGDSEEQAALVLGASGWQMFWRVTLPNIKWTLLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTIPLLVEIFYNEYNFTGAFSLSGVLALLALATLAVQNIITKL"
     gene            complement(15905..16741)
                     /gene="cysT"
                     /locus_tag="WM481_001042"
     CDS             complement(15905..16741)
                     /gene="cysT"
                     /locus_tag="WM481_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFA9080372.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     DGFRNTIAEPNVLAAVRLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALAALYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGSIPMISEILPLIITGKLEQFDAQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            16922..17254
                     /locus_tag="WM481_001043"
     CDS             16922..17254
                     /locus_tag="WM481_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HFA9080373.1"
                     /translation="MQILSFQADIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDAWLEAAKDCGHH
                     GDACACGH"
     gene            17585..18094
                     /locus_tag="WM481_001044"
     CDS             17585..18094
                     /locus_tag="WM481_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFA9080374.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTV
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(18326..18907)
                     /locus_tag="WM481_001045"
     CDS             complement(18326..18907)
                     /locus_tag="WM481_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019957326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFA9080375.1"
                     /translation="MEHKLPQLPYEPDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPVGGLDLISTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLEGFWEIVNWDEVAKRFAA"
     gene            19071..20477
                     /gene="dnaB"
                     /locus_tag="WM481_001046"
     CDS             19071..20477
                     /gene="dnaB"
                     /locus_tag="WM481_001046"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFA9080376.1"
                     /translation="MNDYAAMPPEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINEGRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGRLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEYVAIEKHLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPNLTALELRARARRLARRFDGRLGLI
                     VIDYLQLMAASGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVEQRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQESQMKGYAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT         4814 a         4898 c         5855 g         5052 t
ORIGIN      
        1 ggcaagttcg gaacggctgt tttcgctgtt caggtaggta agggaagttt ccagtttcca
       61 tttgcccttg tcggtcatta tgtcttcaat cgtcaagggc aggtcggcat aagcggataa
      121 aggcaggatg gcgggcaagg cgggcaaaaa gatacgtttc atatttcctc ttattgatat
      181 taattcttat ttaataatag taatgaatat ccgataattc tgtttaaaca aatttttaaa
      241 tcaaaaaaaa acgatatatt tgtttttatc aattatgttt tgatatgccg tctgaaaagt
      301 ttgtgaaaaa cggttacaat ccgccgcatg aaaaaacgca ataatcctct tccgctgttg
      361 aacggtgtca aacccagtta tttggtgctg ccgcatgaaa agcagtttta cgggctgccg
      421 ctgctgcatt ttctgtgcat ccgctttcct tttttgggag cggacgattg gcggaggcgg
      481 ttgaacagcg gttttgtggt cggttcggat ggtgcggcgt tggacgaaca ttctttgttc
      541 gagcccggta agacgatgtt ttattaccgt gaaaccggtt gtgagagcga gccgcgtatt
      601 ccgtttgaag aaaagatttt gtttgtcgat gaacatttga ttgtggtgga caagccgcat
      661 tttctgcccg tcatccccgg cgggcgtttt ttgcgggaaa cgctgctcac gcgtctgcgc
      721 ctgcgtcccg aattgcagca tttgaatgtt gaagacatta cgccgattca ccgcttggac
      781 aaggatacgg cgggtgtgat gctgctgtcg cacaatcctg ccacacgcgg ggcttatcag
      841 acgatgtttc aaaacaaaac ggtatggaaa acgtatgagg cgcttgcgcc gacaaagacg
      901 gatttgccgt atccgctcga tgtggtttcg cgtttggtaa ggggtgagaa attttttacg
      961 acgcaagagg ccgaaggcgt gccgaatgcg cacacgacgg tcgaattgat tgaaaacagg
     1021 ggggaattca gcctttaccg ccttacgccg cataccggca agaaacacca gttacgcgtg
     1081 catatgatgg gtttgggtat gccgctgttg aatgacgcgc tctatcccgt gccgtctgaa
     1141 gcgggtagcg aggattatcg gaaacctttg aagcttttgg caaaaaagat tgcgtttgca
     1201 gatcctttgt cgggtcggga aagggtgttt tgcagcgggt tttgcctgta atgagaggga
     1261 acgcaaaccc gtatcggcag atgccgccgg atacgggttt gatattatcg atgctgtctg
     1321 aaaggggctt tatccgttca ggcatacggg tgcgaagcgg aggatggtgt cgcgcaggac
     1381 gatgagtttg ccgtgaaaga agtgcgtcga accggcgatg gtaatgacgg gcaaatcttg
     1441 cggttctgcc catttcaggg ctttttcgat ttcgacgacc tcatcttccg cgccgtgtat
     1501 catcagcgtt ttggcaacgt tgggaacggc ggacggctcg gggcggtcgg tatagtggca
     1561 gactgccgca ccgatgagca gcaataaatc gggtatgcgc acttgtgcgg caaatgtggc
     1621 gacatagccg ccgaaggaaa agccggataa ggcaaattcc ggggcttcgg gatgttgggc
     1681 gcgggcatag tcgatgacgg cgaggcagtc ttgcgtttcg ccgcgtccgt aatcatgtgt
     1741 gccttcgctg ccgcctacgc cgcgaaggtt gggaaggtag cagtggaagc cgagcttgct
     1801 caatgctttg gcggcggttt ggatgacttt gttggtgttc gtcccgcctt ggagggggtt
     1861 ggggtggttg atgacggcaa caccgcgtgc cggtacttgt tcggacggga tatggatggt
     1921 ttctaaaatg ccggcagggc cgggtatgtg tatggtttcg ggtttcagca taatggtttc
     1981 ctcttgattt tccgcatatt ccgatgcagc cgcgaagatg gcggcttaaa ggtggaaatg
     2041 cttgccgttg ttggggttgg acgctttcag gccggcgagc atgcggtgga agtccaatcc
     2101 gtatgcgttg ctcagtttgt cggcacgttt tttgagtttg tctatgtttt gctctttggg
     2161 gctgctttgg aaggccatta ccgcgacatt gccgtggctt tcggcaggaa gtttcaatac
     2221 gcgcccttca aaaacgctca acaaccgttc gatgaagcgt tgatagcgtt tgtcgccgct
     2281 ccaccagttg gtaacgaata tgccgtctga agagagtgcg ttgcggcagt ctctgaaaaa
     2341 cggttcttca accagcgtat cgatgatttg ttcgccgtcg aatccgtcca ccaagatgat
     2401 atcggtgttg tggcggaaga ctttgatgta ttccgcaccg tctgcttcga taatttcaaa
     2461 ttttccgccc tcgaaaggca actcaaacag gttgcgggca atggcgatga cctgaggatt
     2521 gatgtccacg gcggtttggc gcgtgtcggg caggtaggtg tctgtccagc gtgcaaacga
     2581 accgccgccc aagccgattt gggtgatatg ttgcggcagg cgttccgcaa acagcagcca
     2641 gcccatcatc gcgcggctgt aagagagcac cagctcggac gggcggtcga ggttcatcga
     2701 gctttgaacg gtgtcgctgc ccaagtgaag cgaacggata ttgccttctt cggaaatacc
     2761 gacttcggga aagccggatt ttgcaggacg caggcggcgg tagggatgtc ttgccatcgg
     2821 tgtgaacggt cggtttgaaa ggcggatatt ttatcagaat gccgatgctt gttctgtttc
     2881 aaattaattt tgttttaaat aagatactta gtgtattttt atgagggggt cgaacaaatt
     2941 tcttgccaaa gtgtgaagtt gcctgcataa ttcgccttct ttgccggtat agctcagttg
     3001 gtagagcacc tgacttgtaa tcagggggtc ccgagttcga ctcttggtgc cggcaccaga
     3061 tttgacagcc cgattgtgta aaacagtcgg gctgttttgc atttgcggca cggtcggtgg
     3121 cagggctgcg ggcatttcac tataatagcc cgtttcaaac tgaaaaggcc gtctgaaaag
     3181 ggcggggtaa caatatctga tgattactgt gaacacactg caaaaaatga aggcggccgg
     3241 cgagaaaatc gttatgctga ccgcttacga atccagtttt gccgcgctga tggacgatgc
     3301 cggcgtggat gtgctgttgg tcggggattc tttgggaatg gcggttcagg ggcgacagtc
     3361 gacgctgccg gtcagcctgc gcgatatgtg ctatcacacc gaatgtgtag cacgcggtgc
     3421 aaaaaatgcg atgattgtca gcgatttgcc gtttggtgca tatcagcaga gtaaggagca
     3481 ggcgtttgcc gccgccgccg aactgatggc tgccggcgcg catatggtca aactcgaagg
     3541 cggcgtgtgg atggcggaaa cgactgaatt tctgcaaatg cgcggaattc cggtctgtgc
     3601 ccacatcggt ctgaccccgc aatccgtgtt tgccttcggc ggatataaag ttcaggggcg
     3661 cggcggcaag gcgcaggcgt tgcttaacga tgccaaggcg catgatgaag ccggagctgc
     3721 cgtcgtactg atggaatgcg tgccggcgga actggcaaaa aaggtaactg aaactgtttc
     3781 ctgtccgacc atcggcatcg gggcgggcgc ggattgcgac ggtcaggttt tggtgatgca
     3841 cgatatgctc ggtattttcc cgggcaaaac ggcgaaattc gtcaaaaact ttatgcgcgg
     3901 gcaaagcagc atccaggctg cggttcgggc gtatgttgcc gaagtcaaag ccaaaacctt
     3961 ccctgctgcg gaacatattt ttgcagattg aacaaggtct gctgccgatg ccgtctgaaa
     4021 gccgtttcag acggcatttt gttttgccgt gcgcggcgcg tataatcggc gcgttttgtc
     4081 gggcaggaag cccgaaggat aaggattacc gtaatgcaaa tcatacatac cattcgagaa
     4141 ctgcgcacgt ggcgtgaaaa tataggaaag gtggcatttg tgccgaccat gggcaatctg
     4201 catgaagggc atcttgccct tgttcgcgaa gccagaaaac gcgccgacaa cgttgttgtc
     4261 agcatattcg tcaaccgctt gcaattcggt cagggcgagg atttcgacaa atatccgcgt
     4321 actttgcaac aggatgcgga caaacttgcc gccgaaggcg ttgccgttgt tttcgcgccc
     4381 gatgagaaag aactctatcc gaacgtggaa cagcgttaca acgtcgaacc gccccatttg
     4441 caaaacgaat tgtgcggcaa attccgcccc ggccattttc gcggcgtggc gacggtggtg
     4501 tccaagctgt tcaatatcgt tttgccggat gttgcctgtt tcggcaaaaa agattaccag
     4561 cagcttgccg tgattaaagg tttgaccgag gatttgaatt tcgatatcga aattgtcccc
     4621 gtcgatacgg ggcgggcggc ggacggtttg gcactctcca gccgcaaccg gtacttgagc
     4681 gttggggaac gcgccgaagc accgcgcctg taccgcgagc tgcaggctgt tgccgaatcg
     4741 ctgaagcagg gcggtttgga ttatgcgggt ttggagcggc aggctgccga ccatttgacc
     4801 gctgcgggct ggctggtcga ttatgtggaa atccgccgcg ccgatacgct cgaaatggcg
     4861 cgggcgggag ataagaaact ggtggtcttg gccgccgccc gtctggggac gacgcgtctg
     4921 attgacaatg tggaagtcgg cctgccttag cccgccggag ccgggaatgc cgcctgaagc
     4981 ggatttgcgt ttcagacggc attttgccgt cggctgtgct tcggaataat gtcggaaaat
     5041 agacggaatg aaataaattt ctttttgttc aacaaattaa actgtcaaac caaataataa
     5101 ggcaaggatt gtccggcatc gcatacaggc aaaccgacgc aatctcaaaa cacagccctg
     5161 ccttcgggcg ggttttgcaa atctacaaac cgtttggctt gtgataaagt gacgccttgt
     5221 ctagatactc aatatgccgt ctgaaggttc ggacggatgt cggataaagg atgattatgt
     5281 tacccgcccg tttcactatt ttatctgtcc tcgcagcagc cctgcttgcc ggacaggcgt
     5341 atgctgccgg cgcggcggat gtggagctgc cgaaggaagt cggaaaggtt ttaaggaaac
     5401 atcggcgtta cagcgaggaa gaaatcaaaa acgaacgcgc acggcttgcg gcagtgggcg
     5461 aacgggtcaa cagggtgttt acgctgttgg gcggtgaaac ggctttgcag aaagggcagg
     5521 cgggaacggc tctggcaacc tatatgctga tgttggaacg cacaaaatcc cccgaagtcg
     5581 ccgaacgcgc cttggaaatg gccgtgtcgc tgaacgcgtt tgaacaggcg gaaatgattt
     5641 atcagaaatg gcggcagatc gagcctatac cgggtgaggc gcaaaaacgg gcggggtggc
     5701 tgcggaacgt attgagggaa gggggaaatc agcatctgga cgggttggaa gaggtgctgg
     5761 cgcaatcgga cgatgtgcaa aaacgcagga tatttttgct gctggtgcaa gccgccgtgc
     5821 agcagggtgg ggtggctcaa aaagcatcga aagcggttcg ccgtgcggcg ttgaaatatg
     5881 aacatctgcc cgaagcggcg gttgccgatg cggtgttcgg cgtacaggga cgcgaaaagg
     5941 aaaaggcaat cgaagctttg cagcgtttgg cgaagctcga tacggaaata ttgcccccca
     6001 ctttaatgac gttgcgtctg actgcacgca aatatcccga aatactcgac ggctttttcg
     6061 agcagacaga cacccaaaac ctttcggccg tctggcagga aatggaaatt atgaatctgg
     6121 tttccctgcg taagccggat gatgcctatg cgcgtttgaa cgtgctgttg gaacacaacc
     6181 cgaatgcaaa cctgtatatt caggcggcga tattggcggc aaaccgaaaa gaaggtgcgt
     6241 ccgttatcga cggctacgcc gaaaaggcat acggcagggg gacgggggaa cagcggggca
     6301 gggcggcaat gacggcggcg atgatatatg ccgaccgcag ggattacgcc aaagtcaggc
     6361 agtggttgaa aaaagtgtcc gcgccggaat acctgttcga caaaggcgtg ctggcggctg
     6421 cggcggctgc cgaattggac ggaggccggg cggctttgcg gcagatcggc agggtgcgga
     6481 aacttcccga acagcagggg cggtatttta cggcagacaa tttgtccaaa atacagatgc
     6541 tcgccctgtc gaagctgccc gacaaacggg aagccctgat cgggctgaac aacatcatcg
     6601 ccaaactttc ggcggcggga agcacggaac ctttggcgga agcattggca cagcgttcca
     6661 ttatttacga acagttcggc aaacggggaa aaatgattgc cgaccttgaa accgcgctca
     6721 aacttacgcc cgataatgca caaattatga ataatctggg ctacagcctg ctttccgatt
     6781 ccaaacgttt ggacgagggt ttcgccctgc ttcagacggc ataccaaatc aacccggacg
     6841 ataccgccgt taacgacagc ataggctggg cgtattacct gaaaggcgac gcggaaagcg
     6901 cgctgccgta tctgcggtat tcgtttgaaa acgaccccga gcccgaagtt gccgcccatt
     6961 tgggcgaagt gttgtgggca ttgggcgaac gcgatcaggc ggttgacgta tggacgcagg
     7021 cggcacacct tacgggagac aagaaaatat ggcgggagac gctcaaacgc tacggaatcg
     7081 ccttgcccga gccttcccga aaaccccgga aataatgcag gcccatcctt tcagacggca
     7141 taaggtttgc cgggaagccg gggcattcgg gcaaacggca cgcagttcgc acgcgttttg
     7201 cacggcacgc cgcacccgtc ggccggcagg atgacacccg ttaaggaaac tctgatgaaa
     7261 cacaccgtat ccgcatcggt catcctgctt ttgaccgctt gcgcgcaatt acctcaaaat
     7321 aacgaaaacc tgtggcagcc gtccgaacac atcagcagtt ttgcagcgga agggcggttg
     7381 gcagtcaaag cggaagggaa aggttcgtat gcaaatttcg attggacata ccaaccgccc
     7441 gtggaaacca tcaatatcaa cacccctttg ggcagtacgc tcggacagtt gtgtcaagac
     7501 agggacggcg cattggcagt ggacggcaaa ggaaatgtct atcaggcaga aagtgcggaa
     7561 gagttgggca ggcagctggt cggtttcaaa ctgccaatcc aatatctgca tatctgggcg
     7621 gaaggcaggc gtgtggcggg cgcgccttac cgcatccgtt cagacggcat attggaacaa
     7681 tacggttgga caatcggcag aaccgccgac agtggggggc aagtccgaac gttgcaactg
     7741 aataacggaa atttgaacat caggctggtt ttcaccgaaa ttggaatgcc gtctgaaacc
     7801 gaaaccccgg aacgctgtgc ggcgcgcacg agataaggcg gacagatgaa tattgcggac
     7861 ggacggcagg cgtttcccgc acctgcaaaa ctgaatctcg atttgaggat taccggcagg
     7921 cgggaagacg gttatcacaa tatcgaaagc atattctgcc tgatagattt gcaggatacc
     7981 gtatatttga aaccgaggga cgacggcaaa atcatcctgc acaatcctgt cggcggaata
     8041 ccgcaggaag ccgatttgag ctaccgtgcc gcatcgttgc tgcaaaaata tgcgcgcaac
     8101 cttgccggcg tggaaatatg gctggacaaa aaaatcccga ccggcgccgg cttgggaggg
     8161 gggagttccg atgccgcaac ggttttgctg gtgttgaacc gttggtggca gtgtggtttg
     8221 acgcagtggc agctcattga tttgggtgcg gctttgggtg cggacgtacc gttttttatt
     8281 ttcggaaaaa atgcttttgc aagcgggata ggtgaaaaac tgataggaat ggatattccg
     8341 aaacagtggt atgttattgt caaacctccc gtccacgttt ccaccgcaaa aattttcaca
     8401 tacgaaggct tgacacggga ttctgcctca agcataatgc cgactttcca aaacctgcaa
     8461 ccgttcagaa acgatatgca ggcagtggta tttaaagaat accctgaagt ttggaaagct
     8521 tattccgagt tgtcgaaata cggttctgca atgatgaccg gctcaggagc ctgtatattt
     8581 gcagcatttc aagctaggaa tagcgcatac aatatatacc gacaagtttc aggtttgtac
     8641 gaggcatatt tggcagaggg tctttcaaaa catcctttgt tgtccgtata aacattgttg
     8701 gggagtcgtc aagcggttaa gacactggat tttgattcca gcatgcgaag gttcgaatcc
     8761 ttcctcccca gccaagccaa acaagttggg gagtcgtcaa gcggttaaga cactggattt
     8821 tgattccagc atgcgaaggt tcgaatcctt cctccccagc cagacaaaag cgtgtaagcc
     8881 tgcttacacg ctttttattt catagaaata aaaatattga aacgcctttg tttgtgtcgg
     8941 atgttgcagg tataatgccg ggcttggtac aagcggaggg aagcattgtg ttttctgagc
     9001 ggaagttaaa cataaaatca ggtgagaata tggctgcgta cgacagtttg atggtattta
     9061 caggcaatgc caatcccgaa ttggcacaac gtgttgtcag gcatttggac atttctttgg
     9121 gcaatgcttc cgtatccaag ttttcagacg gcgaagttgc cgtcgaactg ttggaaaacg
     9181 tacgcgggcg cgacgttttc atccttcagc cgacctgtgc gccgaccaat gacaacctga
     9241 tggaaatcct gacgatggcg gatgcactga agcgtgcttc ggcaggtcgt attaccacag
     9301 ccattccgta tttcggctat gcgcgccaag accgccgtcc gcgttccgtc cgcgttccga
     9361 tttctgccaa actggtggca aatatgctgt attcggcagg gatcgaccgt gttttgactg
     9421 tcgatttgca tgccgaccag attcaaggtt tcttcgatat tccggtggac aatatttatg
     9481 ccaccccgat tttgttgaac gacatcaagc agcagcggat tgaaaatctg accgtcgtca
     9541 gcccggacat cggcggtgtc gtccgcgccc gcgccgttgc aaaatccctg aatgccgact
     9601 tggcaatcat cgacaaacgc cgcccgaaag ccaatgtggc ggaagtcatg aacatcatcg
     9661 gcgatattca aggcaaaacc tgtctgattg tggacgatat gattgacact gcaaatacgc
     9721 tgtgcaaagc cgccgtcgcc ttgaaagagc gcggtgcaga acgtgtcttg gcttatgcca
     9781 gccacgctgt attctccgga gaggcggtca gccgtattgc ctcatccgaa atcgaccagg
     9841 tggtcgtaac cgataccatt cctttgtctg aagcggctaa aaactgcgac cgtatccgtc
     9901 aggtaacgat tgcaggtttg ttggccgaaa ccgtccgccg cattagcaat gaagaatccg
     9961 tctcatatct tttcaatgaa gaagtgatga caggcagcat gttgctgccg taaccccgat
    10021 gccgtcttaa gctggtcgcg gccgatgacg gcgattttac ctaacttgga gtatttaaca
    10081 tgacttatga aattcaagcc tctgttcgtg aagcacaagg cactggtgcg agccgccgcc
    10141 tgcgtcgcga aggccaaatc cccggtattc tgtacggtga aggtcaagag cctgttgcca
    10201 ttgccgtaga tcacaaaacc gtattttacg cattggaaaa agaatctttc catactgcgt
    10261 tgattaaatt gtctctgaac ggcgaaacca aagacgttat cgtccgcgat ttccaaatgc
    10321 acccgttccg ccgcgaagtc caacacatcg acttccaagc tgtgaaagcc gatcaacttg
    10381 tacgcatccg cgttcccctg cacattgtta acgctgaaaa ttctcaagcc gttaaattgc
    10441 aaggcggtcg cgtatctctg ctgaacacgg ctgttgaagt ggttgcgttg cctgccaata
    10501 tccctgcttt cttggatttg gattgtgccg aagtggttgc cggcgacatt ttgcacttgt
    10561 cagacatcaa actgcctgaa ggtgtagaaa gcgtttccct gaaacgtaac gaaaatctgg
    10621 ctgttgctac cgttaccggt aaaaagcgct aacttacttc catcagcagg gcggcgcgta
    10681 tgcaatacgt accgccctgt tgttttatgc cgtctgaacc gtgtttcaga cggcatttct
    10741 ttatttgttg gaaaagcggg atatttgaaa cggcagatta ctgccctgtc agacgcgtcc
    10801 aaagcctttg ccaccggctt cttttttcta cgttttccag tgcgacgatt tctttttcgg
    10861 caatggtatg tccgttttgc ctgattttga tttttcctaa aatctgccct ttttttaccg
    10921 gggcgggaat cggctgtatg gtttccaaaa tctgttctgc cattttcgct tctttatgtg
    10981 gcagagtgat gtaggcttct ttgaggaagc ctgcgcggac ggtttttttg ctgcctccgg
    11041 aaatttggat ttgggcaacg gttttgcctt tcggatatat tttgggcgta tcgaaggcct
    11101 gcaatgcccg gttcagcagc ttgctgttgt ccgatgcgcg ggtttccgcc gattccgaac
    11161 ctagtgtgat gacaaggatg tgcctgccgt tgccggagta tgacacggca aggttgtagc
    11221 cgccgctttc tgtgtgcccg gctttcaggc cgtttacatt gttgtcccta tataaaagga
    11281 tattgcggtt gttttgttct atgttttcaa acttgaacga tttgatggaa aacagcgggt
    11341 aatattccgg aaagtcgcgc atcaatgctt cagacagcag ggagaggtct ttggcggtgg
    11401 aaacctgtcc ttctctaccc aagcctgtcg ggtttttgaa tacggtgttc ttcatgccca
    11461 agcgtcgggc ttctttgttc atttgttgca caaaattttc aatcgagccg ttgcccagcc
    11521 ggtcggcaag ggttagggcg gcatcgtttg cgcatagggc aatcatgcct tttaagagtt
    11581 tgtcggtgct gaccgtatcg ccgggacgta caaacattct gcttccttct gaagcccatg
    11641 cggattcggg tatttttaag ttttcttcag attggatatt tcccgatttc atgtttttga
    11701 aaaccaaata tgcggtcatc agttgggtta gtgccgccgg ttcgacaggg gtattggtgt
    11761 ttttggcgga taaagtctgc ctgctttgaa ggtcgataac gatgtgtgcc gccgtgaggg
    11821 tttcgggtgt ttggagcgtg ggggcggcgt gtaccgtcgg tctgttgggc gcgggcgatg
    11881 cagccgttgc gtgagaaacg cctaagatga tgggaagaag gacgggcagg attttatgtg
    11941 ctgtcatgaa atattctaaa ttgtgtgagt gtttcagcct gccgattata cgcttagggt
    12001 gtctgatcgg gcggattttt cttgctttcg cgccgtcttg ggcgtatggt tttgggtttt
    12061 gcgattttaa taaaccgatt atcccgcatt gaattatgaa cacgcccctt ccttattccg
    12121 attacctcat ccgcatcctg acggcatctg tctatgatgt agcggtcgaa acgcctttgg
    12181 aaccggcacg cagcctttct gtacgtttga aaaacaacat ccttttgaaa cgcgaagatt
    12241 tgcagccggt tttttcgttc aaaatacgcg gcgcgtacaa caaaatgtcc aagttgccga
    12301 aagatgcgct cgcttgcggc gtgattgcgg caagcgcggg caatcacgca caaggcgtgg
    12361 cgttgtccgc acagcgtttg ggctgccgtg ccgttatcgt catgccggag actacgccga
    12421 aaattaaagt ggatgcggtt aaaagccgtg gcggcgaggt ggttttgcgg ggtgtttcat
    12481 acaacgatgc ctacgattat gcgatggagc tggcggaaca ggaagggctg acctatatcg
    12541 cgccgtttga cgatcccgat gtgattgcgg gacaggggac ggtggggatg gaaattgtca
    12601 gccagcatcc cgatccaatc cgcgccgtat tcgtaccgat agggggcggc ggtttggcgg
    12661 cgggcgtggc ggcatttatc aaacaggttc gtcccgaaat taaagttatc ggcgtacaga
    12721 ccaacgattc ctgctgtatg aagcagtcgg tcgaagcggg cgaaatcgtc catttgaaag
    12781 atgtcgggct gttttcagac ggtacggcgg tcaaagtcgt cggaaacgga accttccgcc
    12841 tctgcaaaga acttttggat gaaatcatta cagtcgatac cgatacggtt tgcggcgcgg
    12901 tcaaggatat tttcgatgac acgcgcagca ttaccgagcc ggccggggcg ttggcgttgg
    12961 cgggtctgaa agcctatatt gcccgagaag gtgcggaaaa ccaaaccctg attgccgtta
    13021 ccagcggtgc gaatatgaat tttcaccgtt tgcgccacgt ttcggaacgg agcgaattgg
    13081 gcgagggcaa cgaaggtatt tttgccgtta ccatccccga agaacgcggc agcttcctta
    13141 agtttgtcaa tatattggga aataggaata ttaccgagtt caactaccgc tacggcgacg
    13201 atgaaaaagc gcatatcttt gtcggacttc aagcggcagg cccgcaggat ttggcggtta
    13261 tcggcagccg gttggatgag gcgggattgc ccaatgtcga tttgaccaat aatgagattg
    13321 ccaaaatcca tatccgctat atggtcggag ggcggacgga caaagtaggg cacgagcgtc
    13381 tggtcagttt tgagtttccg gagcgtccgg gcgcgttggc gcgctttttg aaccatatgc
    13441 agggcggttg gaacattacg ctcttccatt accgcaacca cggtgcggat tacgggcgga
    13501 ttttggtcgg tatcgacgtg ccgccgcacg atgccgccgc atttgacggt ttcttggaaa
    13561 gtctgggata cagctatcac gaggaaacgc aaaatgccgc gtacaagctg tttcttgcct
    13621 gacgtttgac acgcaatgcc gtctgaaagc ctttcagacg gcattgcgct ttcagggtta
    13681 aatcgaatat tcaatcaact cgttttgaga aaaaacataa acctgtttcg gaattaagtt
    13741 taattttttg ccttcggcga ttgggtagcg cgcggcatcg ctgccagcca gtgtgatatg
    13801 cacgtcttgt ttgccgtgtt tcaccaaaac gtgcgtcaat gcgccgacgg cgtggatttt
    13861 ttcgatttcg gcacggatca tcggggtttc gtgttcggcg gcgatctgcc attcgtgcgg
    13921 gcggatatag ccggtggcgg tttgctcctg ccatttgtat tgcgcgtcca atttccacgc
    13981 gaagccgttg taatgccaga tccctttttc gatgcgtcct tcaaatgcgt cggcttcgcc
    14041 gaggaactcg gtaacgaagg cgttttcggg tttgcggtaa atggcttcgg cactgccggt
    14101 ttgttcgatt ttgccatggt tcatcacgac gatttcgtcg gaaacttcga gggcttcttc
    14161 ttggtcgtga gtcaccagaa tgctggttac gccgaggttg tgatgaatgt cgcgcagcca
    14221 ggtgcgtaat tctttgcgca ctttggcatc cagcgcgccg aagggttcgt ccaaaagcag
    14281 gagtttcggt tcgaccgcaa gcgcgcgggc gagggcgatg cgctggcgtt gcccgccgga
    14341 gagttggtgc ggataggatt tggcgaggtg ggagagttgt acgagtttga gcaattcttc
    14401 gactttggcg cggatttgtc ctttggacgg gcgttcgggc ttgggcaata cggtcaaacc
    14461 gaaagcgacg ttgtcaaaca cattcatgtg gcggaagagg gcgtagtgtt ggaacacgaa
    14521 accgactttg cgctcgcgca catgcttggc ggttacgtct tggccgtcaa agagaatttg
    14581 gccgccgtcg gcgttttcca gtccggcgat aatgcgtaaa agtgtggttt tgccgcagcc
    14641 ggagggaccg agcagggaaa cgagtttgcc ggcggggacg ttgaggttga tgtttttcag
    14701 cgcgtgaaaa ttgccgaagc gttttttgtt taagttttga atggtaatac tcatactgcg
    14761 ttcctttcgg cggcggcgag ttttttgtct tgtatagtgg attaaattta aaccagtacg
    14821 gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    14881 ttaaatttaa tccactataa tttggtgatg atgttctgca ccgccagcgt cgccagtgct
    14941 aaaagtgcca atacgccgga gagggagaat gcgccggtga agttgtattc gttgtagaag
    15001 atttcgacca aaagcgggat agtgttggtt tcgccgcgta tgtgtcccga taccacgctg
    15061 accgcgccga actcgcccat cgcacgggcg ttggtgagga tgatgccgta gagcagcgtc
    15121 catttgatgt tgggcagggt aacgcgccaa aacatctgcc aaccgcttgc gccgagtacc
    15181 aatgccgcct gttcttcgct gtcgccctgt gtctgcatta aagggatgat ttcgcgtgcg
    15241 gcaaagggaa aggtaacgaa cagcgccgtc aaaacaatgc cgggaatggc gaagataatc
    15301 tgtatgcctt gcgcttccag ccggctgccc aacgccgtgt gcgcgccgaa caataagacg
    15361 aacatcaaac cggcaaccac gggcgatacg gaaaacggca aatcgagcag ggtggtcagc
    15421 aactgcttgc cgagaaagtc aaaacgggtc aacagccacg ccatcgccac gcccaagacg
    15481 gcattgacgg gaacggcaat cagcgcggta atcagcgtca atttgacggc agaccacgct
    15541 tcgggatcgc ttaaggattt caggtacaaa tcccaaccgc cttttaaggc ttcgtagaat
    15601 acggcgacga gcggcacgac cagcatcagc agcagaaagc ccagcgcggc ggcaatcagc
    15661 aacatgcgca gccggcgcgg ttcggtcagg ttgggattgg cggaataggg tttcatggcg
    15721 gtggtgttct ctggtttgtt gtttaaggtg ttcggatgcc gtctgaaagg gattgagggg
    15781 tttcagcttt cgtcggtcgg attcttgaat ccgacaaggt tgctgaatcg caaatgtttg
    15841 tcggatacaa gtatccgacc tacgaattct gtgttttggg gagcggattc gttttcagac
    15901 gacctcaacc cttcgcaccc gaacgcctgc ccaacgccca ctgcatcacg ttcagcgcaa
    15961 acagaatcac aaacgaaacc agcagcataa acaacgccac cgccgacgcg ccctgcgcgt
    16021 cgaattgttc cagcttgccc gtaataatca gcggcaggat ttcggaaatc atcggaatgc
    16081 tgcccgcgat aaaaatcacc gaaccgtatt cccccgttgc ccgcgcgaac atcatgcccg
    16141 cgccggtcaa gagtgccggc gtgatttccg gcagcagcac gcggcggaac gttgtgaaac
    16201 ggtttgcgcc caaagtcgcc gccgcttcct catattcgcc cgacaattcc tccaataccg
    16261 gctgcacggc gcggacgata aagggcaggc tgacgacaac cagcgcaatc caaatgccga
    16321 cgggcgtaaa cgcgattttg atgcccaaag gttcgaaaaa acggcctatc caaccgttgg
    16381 gcgcatacag ggccgccaac gcgatacccg taaccgccgt cggcagcgca aacggcaaat
    16441 caaccagcgc gttcgccagg cccttgcccg gaaattcata gcgcaccaac acccaagcca
    16501 ccagtgtgcc gaacacgaca ttggtcagca tcgcataaaa cgacatccgc aaactcagcc
    16561 gcaccgccgc cagcacgttc ggctcggcaa tcgtgttccg aaagccgtcc cagccgattt
    16621 ccgccgcctt cgccgccatc atcgcaaacg gcaaaaccac cagaagcgac aggcacaata
    16681 cggtcagacc gaggctgagt ttgaagccgg gcagtacgcc gggtgttttg agcggtaaca
    16741 tggtcggtaa gggtggaaaa taaaggcatt actttaacaa tgccgcctga aaaacggaaa
    16801 aaatggaaag tttggtgcaa agacgaattt gttataaagc ggttggcagt ttcttaagcg
    16861 ggcgcgatgt tctaaaataa cgctttgttt tgacggaaaa ccatcatata aaggaacact
    16921 tatgcagatt ttatcttttc aggcggacat tgcggaacgt atgctggaag gtacggaagg
    16981 cgagtcggtc aacgaaaacg cacaatttgt ccgtacggac aacggctatt ggattgcgtg
    17041 gcatgaaggc gtggcggcac tgcttgcgcc cgatacgccg ccgggcattc cctgtttttg
    17101 ggtggaaggg gcggaaagcc ttgaagagtt gtgcgcgatg gtggaacgcg gcgagtttga
    17161 cgaagtggaa gagtttgacg gcgatgacga cgcgtggctc gaagcggcta aagattgcgg
    17221 acaccacggc gacgcttgcg cctgcggaca ttaaaggcat ggcaggcttg ccgcaagggg
    17281 tgcaaggctt tgccgttttt taaataatcg gcggattgca tctgtttgca atgggcggaa
    17341 cgcttccctt tgtattttat tgaataaaaa ttatcttggt attataataa ggcagcatca
    17401 attattttgg gactgcaaca aacgcaaagc attgatttgc gtttgttgca aacttattat
    17461 tatagcaggt tgcggcgcgg acttgatggg aattgtttat agagcatgat agtctgaaac
    17521 atcattaatt aaaaatacca ttaatccgat ttatatttat ttcaatttca atggaaaaac
    17581 atcaatgaca atgattttaa gcattttaag cctgtttttt atcattagac tgttattttt
    17641 agccgtctcc attaaacatg aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa
    17701 aaccaattcc acggtgcttg cggcagttca tacgctttat tatttggcgt gttttgtttg
    17761 ggtatggctt tctgacactg cttttaatgg catatccttg attggtacac tgacggtgat
    17821 ggcttcattt gtgatattat cattgattat taagcagttg ggggagattt ggacggttaa
    17881 aatctatatt ttgccaaatc atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca
    17941 cccgaattat tttttaaaca tcatacccga actgattggc atcgccttat tatgtcaagc
    18001 gtggtatgtt ttattgattg gcctgcccat ttatttgctg gtcttattta agcgtatccg
    18061 acaagaagaa caggcgatgg caacactttt ttaacccgtt tcatcaatta aaaatatatc
    18121 gacaaagaga accgtctatc aattaaatcc attcttatca ttggggcagg cttattgtca
    18181 ttgggcaagc cgtgacaaaa gcccgaacct gcaaaactta tctataaggc tgcatcagtg
    18241 cgtattgaag ccccgccttt tttgacaaaa aaatgccgtc tgaaggcttc agacggcatt
    18301 tgttcggttt ggcaaagccg gcggcttatg cggcaaaacg tttggcgact tcgtcccagt
    18361 tgacgatttc ccaaaaacct tccaggtagt tggggcggct gttgcggtag tcgatgtagt
    18421 aggcgtgttc ccacacgtcg caagtcaaga gcggcgtgtt ttcagtggtc agcggcgtag
    18481 cggcgttgga agtggaaatc aaatccaatc cgccgacagg ggttttcacc agccacgccc
    18541 aaccggagcc gaacgtaccg gccgcgcagg cattgaacgc ttcttggaat ttttcgaagc
    18601 tgccccactt cgcgtcgatg gcggcggcca gttcgccggc aggcttgcct tggcctttgg
    18661 gcgtgaaacc cagccagtag aaggtgtggt tccaagtttg agccgcgttg ttgaacacgc
    18721 cgcccgaaga ttttttcaca atctcttcca aaggcaggtt ttcaaattcg gtgcctttga
    18781 tttgattgtt caggttggtg atgtaggttt gatggtgttt gccgtagtgg aattccaaag
    18841 tctctttgct cagatgcggg gacaatgcgt ccggctcata aggcagttgc ggcagcttat
    18901 gttccatttt gtgctcctaa tgttgttttt ggatgttgtg ttttggcagt gttgctgcaa
    18961 acgaatcggc ggctcacgca ttctacctgt tttgagtgat ggaaaccaat taaacctgct
    19021 ttgcgttata atagaagatt gcaatttcgg catgacagat tggatgtacc atgaacgatt
    19081 acgcagctat gccgcctgaa gaccgtgagg tcggcgcatt gtcgctgcct ccgcattcga
    19141 tggaggcgga acaatccgtt ttgggcggtt tgatgctgga aaatccggct tgggacagga
    19201 ttgccgatgt ggtttcggga gaggatttct accgccacga acaccgcctg attttccgat
    19261 ccattgccaa attaatcaat gagggccgtc ccgcagatgt gattacggtt caggaagatt
    19321 tgcagcggaa cgaagaattg gaagcggcag gcggatttga atatctgatt acactggcgc
    19381 aaaacacccc gtctgccgca aacatccgcc gctacgccga aatcgtacgc gagcgttcca
    19441 ttatgcgcca actcgccgaa gtggggacgg aaatcgcccg cagcgcatac aatccgcaag
    19501 gcagggacgc ggggcggctt ttggatgagg cggaaaacaa agtgtttcaa atcgccgaaa
    19561 gcaccgccaa atccaagcag ggctttttgg agatgcccga tttgctgaaa gaagtcgtac
    19621 agcgcatcga tatgctttat tcgcgcgaca atcccgatga agttaccggc gtaccgacag
    19681 ggttcatcga ccttgataaa aaaacctcgg gtctgcagcc cggcgacctg attatcgtcg
    19741 ccggccgccc gtctatgggt aaaaccgcgt tctccatcaa tattgccgag tatgtcgcca
    19801 ttgaaaaaca tttgcccgtc gccgttttct cgatggaaat gggcggggcg cagttggtaa
    19861 tgcgtatgct cggttcggtc ggacgcttgg atcagagcgt cctgaaaaca ggcaggctgg
    19921 aagatgaaca ttgggggcgt ttgaacgagg cagtcgtcaa actttccgac gcgcctatgt
    19981 ttattgacga aactccgaat ctgaccgccc tcgaattgcg cgcccgcgcc cgccgcctcg
    20041 cccgccggtt tgacggcaga ttaggcttga tcgtcatcga ctacctgcaa ctgatggcgg
    20101 catccggccg ttcggacaac cgcgcttccg agcttggcga aatttcccgt tccctcaaag
    20161 ccctggcaaa agagttgcaa gtccccatca tcgccctgtc gcaattgagc cgcactgtcg
    20221 aacagcgcac cgacaaacgc ccgatgatgt ccgacctccg cgaatccggt gctatcgagc
    20281 aggatgccga cctgattatg tttatgtacc gcgacgaata ctacaaccaa gaatcgcaga
    20341 tgaagggtta tgcggaatgc atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct
    20401 tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg
    20461 caaagataga agattaaatg gctatataaa aatttattag gcggaatcag gcaaaatcgt
    20521 ttaaaatcat gcttagagat tgccctaaaa aataaaacgc ggtcttgagg catttttgca
    20581 ttcagcccgc atataattga aaatatagtg gattaacaa
//
LOCUS       DAWTCI010000028        20461 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWTCI010000028 DAWTCI010000000
VERSION     DAWTCI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20461
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.028"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            150..2177
                     /gene="uvrB"
                     /locus_tag="WM481_001047"
     CDS             150..2177
                     /gene="uvrB"
                     /locus_tag="WM481_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HFA9080377.1"
                     /translation="MEVIRYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDALRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAVDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKENLLFGAE"
     gene            complement(2355..3113)
                     /locus_tag="WM481_001048"
     CDS             complement(2355..3113)
                     /locus_tag="WM481_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFA9080378.1"
                     /translation="MPRFPRTLPRLTAVLLLACTAFSAAAHGNHTHWGYTGHDSPESW
                     GNLSEEFRLCSTGKNQSPVNITETVSGKLPAIKVNYKPSMVDVENNGHTIQVNYPEGG
                     NTLTVNGRTYTLKQFHFHVPSENQIKGRTFPMEAHFVHLDENKQPLVLAVLYEAGKTN
                     GRLSSIWNVMPMTAGKVKLNQPFDASTLLPKRLKYYRFAGSLTTPPCTEGVSWLVLKT
                     YDHIDQAQAEKFTRAVGSENNRPVQPLNARVVIE"
     gene            complement(3522..3869)
                     /locus_tag="WM481_001049"
     CDS             complement(3522..3869)
                     /locus_tag="WM481_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080379.1"
                     /translation="MTYLKVIAISIVLYILLLQINLKMLEKRIDFLVENIDKYYQQYG
                     SYPNNFDFISTKTDFTTESYCDFWDKNIAGYGNCYFVKNDKDYTILVMGFSSKILFSS
                     HNKIKEFNSNKYD"
     gene            complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="WM481_001050"
     CDS             complement(4193..4909)
                     /gene="trmB"
                     /locus_tag="WM481_001050"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFA9080380.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGMASAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVKNMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(5031..6435)
                     /locus_tag="WM481_001051"
                     /pseudo
     CDS             complement(5031..6435)
                     /locus_tag="WM481_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(6552..8438)
                     /gene="uvrC"
                     /locus_tag="WM481_001052"
     CDS             complement(6552..8438)
                     /gene="uvrC"
                     /locus_tag="WM481_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFA9080381.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFCDDKSYPYLMLSGHQYPQMAYYRGTLKNPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYCDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIIQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAITQRHLQQSNQ
                     QHRIDELAKILGMNSDGINRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKIQEAEANGESVKWPDVVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGELFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(8493..10670)
                     /locus_tag="WM481_001053"
     CDS             complement(8493..10670)
                     /locus_tag="WM481_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HFA9080382.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDGSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIG
                     MVGMMLKGLNWTRHDWMIPPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSSHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRTNHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYYVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLEIPAAQNAQTVVGAGITAEVEGVGLVKS
                     GKAEFAELTLPKFSDGVWEIASAVTVSVNGKPIGAFALSDALKADTAEAIGRLKKHNI
                     DVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALLISQATLENIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKWVKID"
     gene            10809..11759
                     /gene="trxB"
                     /locus_tag="WM481_001054"
     CDS             10809..11759
                     /gene="trxB"
                     /locus_tag="WM481_001054"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFA9080383.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFALKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSDQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            11915..12283
                     /gene="rpsF"
                     /locus_tag="WM481_001055"
     CDS             11915..12283
                     /gene="rpsF"
                     /locus_tag="WM481_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFA9080384.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMITEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVGELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLVGAAEEAAAQ"
     gene            12284..12586
                     /gene="priB"
                     /locus_tag="WM481_001056"
     CDS             12284..12586
                     /gene="priB"
                     /locus_tag="WM481_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFA9080385.1"
                     /translation="MGFTNLVSLAALIEKAFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            12593..12823
                     /locus_tag="WM481_001057"
     CDS             12593..12823
                     /locus_tag="WM481_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFA9080386.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            12840..13292
                     /locus_tag="WM481_001058"
     CDS             12840..13292
                     /locus_tag="WM481_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFA9080387.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVIAAAE"
     gene            complement(13915..14721)
                     /locus_tag="WM481_001059"
     CDS             complement(13915..14721)
                     /locus_tag="WM481_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFA9080388.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQTVDWKTIFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPGLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLVNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKESFGIMLLLIAGKMLYNLL"
     gene            complement(14722..15468)
                     /gene="pssA"
                     /locus_tag="WM481_001060"
     CDS             complement(14722..15468)
                     /gene="pssA"
                     /locus_tag="WM481_001060"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFA9080389.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            16051..16533
                     /locus_tag="WM481_001061"
     CDS             16051..16533
                     /locus_tag="WM481_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFA9080390.1"
                     /translation="MMKKILAVSALCLMTAAAQAADTYGYLAVWQNPQDANDVLQVKT
                     TKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVSLAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLK
                     "
     gene            complement(16580..17791)
                     /locus_tag="WM481_001062"
     CDS             complement(16580..17791)
                     /locus_tag="WM481_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HFA9080391.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVTGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTTSIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFVGVGLCAVLAIV
                     LNSLLPDPKES"
     gene            18013..20451
                     /locus_tag="WM481_001063"
     CDS             18013..20451
                     /locus_tag="WM481_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HFA9080392.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTEAKKARPEHVVNLIADALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDA
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALRKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQPSLFEDNEVQQNGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPEEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEQAKAAGKPLLNPFSLNLDEPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGTFVSDGEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGIVSPSD
                     LNGSRKILAHKDHL"
BASE COUNT         4829 a         5153 c         5587 g         4892 t
ORIGIN      
        1 accggtttaa atttaatcta ctatacttgg gtagcgattg ggtttcagac ggcatacatt
       61 caggatttca ccgccttata ccgtctgaaa gacgtttctg acccatccct actacacgct
      121 ttgtccgcaa acgtatcaca ggcaagatta tggaagtcat ccgataccca aattccccct
      181 tcaaactcca ccaacccttc ccgcccgcag gcgaccagcc caccgccatt gcgggcctgc
      241 tcgaagggct ttcagacggc cttgcctacc aaaccctgct cggcgtaacc ggttcgggca
      301 aaacctacac gatggcgaac gtcatcgcgc aaagcggcag acccgccatc attatggcgc
      361 acaacaaaac ccttgccgcc cagctttatg ccgaaatgcg cgagtttttc cccgaaaacg
      421 cagtggaata tttcgtgtcg tattacgact attaccagcc cgaagcctat gtgcccagcc
      481 gcgatttgtt catcgaaaaa gacagcgcga tcaacgaaca catcgagcag atgcgccttt
      541 ccgccaccaa aaacctgatg acgcgcgacg acgtgattat cgtcgccacc gtgtccgcca
      601 tttacggtat cggcgacccg accgagtatc aacaaatggt gctgtccgta aaagaaggcg
      661 acaccatcga gcagcgcgac atcatcgcca cgctcgtttc catgcagtac gaacgcggcg
      721 atttggactt caaacgcggc agcttccgcg tgcgcggcga cgtgattgac gtgtaccccg
      781 ccgaaagctc cgaaaacgcc ttgcgtatca gcctgtttga tgacgaaatc gaccgcctcg
      841 atatgttcga cccgctttca ggcagcctgc accagcgtgt cggacgctac accgtcttcc
      901 cgtccagcca ctacgttacc ccgcgcgaca ccgtattgcg cgcctgcgaa tccatcaaag
      961 aagaattgcg cgaacgcatc gaatttttcg ctcgcgaaca acgccccgtc gagcaacaac
     1021 gcatcgaaca gcgcacccgt ttcgacctcg aaatgctcta cgaaatgggc ttctgcaaag
     1081 gcattgaaaa ctactcccgc cacttctccg gtaaaaaaga aggcgaaccg ccgcccacgc
     1141 tgatggacta cctgcccgac aacgccatca tgttcatcga cgaaagccac gttaccgtta
     1201 cccaaatcgg cggcatgtac aaaggcgacg cattgcgcaa gcaaaacctc gtggactacg
     1261 gcttccgcct gccttccgcc cgcgacaacc gcccgctcaa attccacgaa tttgaaaaag
     1321 tcatgccgca aaccgtcttc gtttccgcca cccccgcgaa atacgaagaa gaacacgccg
     1381 gacaagtcgt cgaacaagtc gtccgcccca cagggctggt cgacccccaa atcatcatcc
     1441 gccccgtcgc cacccaagtc gacgatttaa tgagcgaaat caacgaccgt attcaaaaag
     1501 gcgaacgtgt gctcgttacc acccttacca aacgcatggc ggagcaactc accgactatt
     1561 acagcgaact cggcatcaaa gtgcgctacc tgcacagcga catcgacacc gtcgaacgcg
     1621 ttgaaatcat tagagatttg cggctcggcc tgtttgacgt actcgttggc atcaacctgt
     1681 tgcgcgaagg cctcgacatc cccgaagtct ccctcgtcgc catcctcgat gccgacaaag
     1741 aaggcttcct gcgctcccac cgcagcctga ttcaaaccat aggccgcgcc gcgcgcaacg
     1801 tgaacggcgt cgccatcctg tacgccgaca aaattaccga ctccatgaaa gccgccgtcg
     1861 acgaaaccga acgccgccgc gaaaaacaga ttaaattcaa cgaagaacac ggtatcgtgc
     1921 cgcagcagat taaaaaacag gtcaaagaca tcatcgacgg cgtgtaccac gaagaagaca
     1981 gtggcaaagg ccgtagacaa ggcaaaaaca aggtcaaagt cggcgaaatc cacaacgaag
     2041 aagacgcgat taaagaaatc gccaaactgg aaaaagccat gcagcaggcg gctagggatt
     2101 tacagtttga agaagctgct gtgttgaggg ataggattag gaatattaaa gagaatttgt
     2161 tgtttggggc ggagtgattt tgaaatagta atcgcagcct gaatacatag cgtgtgcgcc
     2221 actggcgcgc gctcattgtg ttatcaggaa tccttgaaat catgtacgca gcttgcatca
     2281 cacatcctag ctatacataa acaacaaggc cgtctgaatt ttcagacggc cttttccgtt
     2341 tacaacttta ctacttattc aataactaca cgtgcattca gaggctgtac ggggcggttg
     2401 ttttccgaac cgacggcgcg ggtgaatttt tccgcttgcg cttggtcgat gtggtcataa
     2461 gttttcaaca ccaaccatga tacgccctct gtgcacggcg gcgtggtcag cgaaccggca
     2521 aagcggtagt atttcaaccg tttcggcagt agggtggatg cgtcgaacgg ttggttgagt
     2581 ttcacttttc ctgcggtcat cggcatgacg ttccagatgg aagacaggcg gccgttggtt
     2641 ttgccggctt catacagcac ggctaatact aaaggctgtt tgttttcgtc taagtggacg
     2701 aagtgagctt ccatcgggaa agtgcggcct ttgatttggt tttcgctcgg cacgtggaag
     2761 tggaactgtt tcagggtata ggtgcggccg ttcacggtca gggtattgcc gccttcggga
     2821 taattgacct gaatggtgtg gccgttgttt tccacgtcaa ccatactcgg tttgtaattg
     2881 actttgatgg cgggcagttt gccggaaacg gtttcggtaa tgtttaccgg agattggttt
     2941 ttgccggtgg agcacaaacg gaattcttct gacagattgc cccagctttc gggagagtcg
     3001 tgtccggtat agccccaatg ggtgtgattg ccgtgtgcgg cggcggagaa agcggtacag
     3061 gccaacagca ggacagcggt caggcggggt aaagtgcggg ggaaacgagg cattgtaaga
     3121 tcctttctat taaggagtag aaaaaaggtt gcagtgtgta tgtaaatatt ttatttttta
     3181 ttaaatgata tctatattaa gtgcttaagt caaacttccc ggtctgaaaa ccattagagc
     3241 aatgaaatta aagtgaattt atttttctat aagtcctgct ttatttggat aaccatgagg
     3301 catggctacc tttatccgga aaaatattaa tagccgcaac aaagatattt gaatccgatt
     3361 caaatatcac cagcaagcgt agctcggata cttgtatccg acaaagcctt taacatttcc
     3421 atcgttgcaa tccattgcag caattaccaa aatgtcggat tcgagaatcc gatctacgga
     3481 aaactaaaga ttttatgcgg gctacggctt gcttgttgta tttaatcata tttatttgaa
     3541 ttgaattctt ttattttatt gtgagatgaa aatagtattt tcgatgagaa acccatgact
     3601 aaaatagtat agtctttatc attctttacg aagtaacaat taccatatcc tgcaatattt
     3661 ttatcccaaa aatcacaata tgattcagtg gtaaaatcag ttttagttga tataaaatca
     3721 aaattatttg ggtaagatcc atattgctgg taatatttat ctatattttc tactaagaaa
     3781 tcaatccttt tttctaacat tttaagatta atttgcaata ataaaatgta taaaactata
     3841 ctaattgcta ttacttttaa atatgtcatt ttattttcca gtaaatagaa gtttagttgt
     3901 tttgttaact atgcctgaat gtacacctaa gtcttctttt ggtaacgatg aataattagc
     3961 aagccgtagc ctgcatctcc gccaaccgcg tgccactatc tgcgggcgga ataaacttta
     4021 aaaccgatgt gcaggttaaa acctacaagg atgcgtgcgg tatacacgcg tttttgccgt
     4081 tcaaaccatt gtttcaaaac ttgtattgaa acatcgcccg ctgctttatc ttgaaagaat
     4141 gccgtctgta agcaaacatg cttcagacgg cattttttta tccggcggtc tgttatccga
     4201 tccgtttgaa taccaagtcc caaacgccgt gtccgaggcg tttgccgcgc gcttcgaatt
     4261 tggtttcggg gcggtagtcc ggagtggggg cgtagtctgc cgccgtattt tgcaggctgt
     4321 cgaagctgct gaggacttca agcatctgct gtgcatattc ttcccagtct gtcgccaggt
     4381 ggatatagcc gccggttttg agtttgggca gtagtttggc gatgaagggg gcttgtatca
     4441 gacggcgttt gtggtggcgt tttttgtgcc acgggtcggg gaagaatatg tggatgccgt
     4501 cgagcgagcc gtcttgcagc atatttttga caacttctac ggcatcgtgc cgcatcacgc
     4561 ggatgttttc caaatggttt tcgttgatga gtttgagcag gttgcctacg ccggggccgt
     4621 gtacgtcgat ggcgagaaag tcggtttcgg gcaggcggcg ggcgatttct gcgcttgcca
     4681 ttcccatacc gaagcctatt tcgaggattt taggtcggct gcttccgaaa cgggcatcaa
     4741 gatcggccgg tgttgcttgg tagtcgatgc cgaagctgtc ccataaggta tcgatggcgc
     4801 gttgctgtgc ggcggtcatg tggctttggc ggaggacgaa actgcggatg ctgcgggggt
     4861 gtccggcttg acggtcgttt ggtgtttggt tttcggcggt gtctgtcata atgcttctgt
     4921 gcgcttgtgc aatcaggatg ccgtctgaat gtgaaaggcc tgttcggacg gcgggaaggg
     4981 aaaggggcga ttatacctgt ttcgtttcgg cagggtaggt gtaactctgt tcagacggca
     5041 ttttgccact gttgcagcag ttcttgcagg acgctttttt gcccgtatcc gttgtttatg
     5101 gcttcttgca ggtagcggca ggctttttct ttgtccgcct ggcaccgtgt ccgctgtaat
     5161 ggatgcaggc gaggttgtag aacgccatac tgtctttttg cgcggcggct tgttcaaacc
     5221 atttgcgggc tttgcagtag tcggcggcta tgccctgtcc gaagtagtac atactgccca
     5281 gattggtttg ggctttgctg tgtccgagtt ctgctgcttc tgtgtagagt ttgagcgcgg
     5341 ctgcgtattc gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagct
     5401 gttctgccct ttgccgccta atggcggaat ctttgatgcc gtcatattgc tcgggatggt
     5461 ttaacgcgct gtcggatatg agtttctgat aggcggtaag gctgccggct tcggctgcct
     5521 gccggtaata atgccgggct ttttccgtat cgagggctat gcccaaaccg tagcggcaga
     5581 tgtcgcccgg tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcatgat
     5641 gcagggcaag tttgtggttt ctttccagat acttgccttg caggtagatg tcggccagtg
     5701 cggcatgggc atcggggtca ttttcctgtg ccgcctgttg gaaccggtaa acggcttcat
     5761 cgggagccgt aggtaggatg agttcggcaa gcggggtgta ggcgggaatg tatccttgtt
     5821 gcgcggcggc gcgcaaatga ttgacggcct gtgccgtgtc ggctttcgtc ccttgtccga
     5881 aacggtattg cagcccgagt tgccaatgtg cttccggcaa gccttgtgcc gccgctttgc
     5941 ggtagagcag gtgcgccgtt tcaaaatccg gcggcgtgcc gtgccggcag tattgggcga
     6001 gcaggtattg cccgtgggga ttgttgtttt ttgcgcttgt tttgcccatt ccagggcttc
     6061 ttcaaaatgt cgggacgcgt acagaaggta catcaggcgg acttgcgctt cgggcaggcc
     6121 tgcttctgcc agaggcctgt aaagcggtat cagggtttcc gggggcgcgc cttccaattc
     6181 ttggatatat gccaaattgt aacgcgcata gggatgcccc tgttcggcgg cgatgcggag
     6241 catttccata ccttgttcgc ggtcttggtg cgggctgtcc gcgttcatca ggtgttttgc
     6301 caactcgaat gtggcggcgg gatgatctgc cgaggccgct tctgtcagca gtcggcggac
     6361 ttcggtaaag ttataatatt gtttttgaga caggcgttcc aaggcctgcc gatagggtaa
     6421 gtcgggattg ttcatatcag atcgttttcc gttttgtcga atcatcgtta ttcgtcatga
     6481 agttaggttt atatcggtat gccggcctgc tgtatcatat ttttacaggc ggtttttgct
     6541 ttggcggcat actaatgcag atgattataa atggtttccg ccaatgcctt gctgatgcct
     6601 tccacttttt ccaagtcctc gcggctggcg gcaatcacgc cgcgcagtcc gccgaagcgg
     6661 gtaagcaggg cttggcggcg tttgctgcct acaccgggaa tgtcgccgag agacgaggta
     6721 acgcgtgctt tgtcgcgttt tttacggtgg cctgtgatgg caaagcggtg tgattcatcg
     6781 cgtacggttt gcaataaatg caaggccggg ctattaggag gcaggcggaa gagttcgccg
     6841 gtaaaaggca gtatgagttc ttccatgccg gctttgcgtt cggggccttt ggcaataccg
     6901 actaaaggga tgtgcagccc gagttcttcc catacggata cggctatgcc gatttgccct
     6961 ttaccgccgt caatcaacac gacatccggc catttgacgc tttcgccgtt ggcttcggct
     7021 tcctgtattt tgccataacg gcgcgtcaac acttcgcgca tggcggcgta gtcgtcgccg
     7081 ggtttggcgg tggtgatgtt gtagcggcgg tattgcgagg gttggatgtt ttgttcgtcg
     7141 tacacgacgc aggaggcgat ggtggcttcg ccttgcgtgt ggctgatatc gaaacattca
     7201 aggcggttga ttccgtctga attcatgccg aggattttgg caagttcgtc gatgcggtgt
     7261 tgttggttgc tttgttgcag gtggcgttgg gtaatcgcca tttgtgcgtt ttgttccgcc
     7321 attttcaacc aaactttgcg ttcgcctatg gttttggtaa cgaactgcat ctgcttgccg
     7381 tgttctcctt ccaaagcctc tttcaacgca tcgggaacgg gaaagttgct gatgatgatg
     7441 tcgggtttgc ttttgcccag atagtgttgg gcgacgaagg cttcggcgta atcttgaccg
     7501 tttggttcgg ggtcgttttt ggtgtcggga aagaagcttt tgtcgccgac gtgccgtccg
     7561 ccgcggatgc tgacccagtg tacgcagaca aggccgtctg aaacggcgag cgcgagcagg
     7621 tcgatatcgt tggggttgtt cgggtttttg ctgtcgatga actggttgct ctgtataatg
     7681 ccgagtgctt ggatttggtc gcgataacgg gcggcttctt caaattgcag attcgcggcg
     7741 gcggtctgca ttttgtgttg cagggtgcgg gtcagttcgt cggttttgcc gttgaggaaa
     7801 gtggcggctt gacgcacgct gtcgcagtag tcttcttcgc tgatgtgtcc gacgcagggc
     7861 gcggtgcagc gtttgatttg gtacagtagg caagggcggt cgcggtgttc gaacacgctg
     7921 tcttcgcagg tacgcagcat gaagactttt tgcaacactt gaatgctgtc gcgcacggcg
     7981 ttgctgttcg gatacgggcc gaagtattgg ttgggatttt tcagcgtgcc gcggtaatac
     8041 gccatttgcg gatattgatg gccgctgagc atcaaataag gatagctttt gtcgtcgcaa
     8101 aaaaggatat tgtatttcgg cgacaaggct ttgatgaagt tgttttcgag aatcagggct
     8161 tcggcttcgg aacgcgtgat ggtggtttcg atgtggcgaa cctgtttcac catcaacgcg
     8221 atgcgcggcg aatggtcgtt tttttggaaa tagccggaca cgcgccgctt gaggttgacc
     8281 gctttgccga cgtataagac gttgccgcct tcgtcaaaaa aacggtacac gcccggcagc
     8341 ttgggcaggt ttttgaggaa gagcgggatg tcgaattgtt ccggagaacg gttttctgta
     8401 ttcatatcaa tcatttgaaa aacggcttca gacggcatat cggcagcggc agccttatcc
     8461 gttcggaaaa gctgtcgggc ggttatatgc ggtcaatcga tttttaccca tttcaggcgc
     8521 agggcattgc ccaataccga aaccgagctt gccgccattg ccgcgcctgc tatgacggga
     8581 tttaaaaagc cgagcgcggc gagcggaatg cccaatatat tgtagaagaa ggcgaaaaat
     8641 aggttttgct tgatgttttc caacgttgcc tgcgatatca gcagggcatc ggcgagctga
     8701 ttgaccgaat gctgcatcag cgtggcggag gcggtgtgtt cggcaacgtc cgcaccgcct
     8761 ttcatggcga agctgacgtt ggcggcggca agcgcgggcg cgtcgttgat gccgtcgccg
     8821 accatcgcca cggttttgcc ggcggctttg agtttctgca cttcggcggc tttgtcgcac
     8881 ggactcatat taccgaaggc gtgtgcgatg cccagttgtt tggcgacgta ttcgaccgta
     8941 ctttggttat cgccgctcat aatatagaca tcgatattgt gttttttcag acggcctatg
     9001 gcttcggcgg tatcggcttt caacgcgtcg gagagtgcga atgcgccgat cggtttgccg
     9061 tttacagata cggtaaccgc actggcgatt tcccaaacgc cgtctgaaaa cttcggcaag
     9121 gtcagttcgg caaattcggc tttgcctgat tttaccaaac ccacgccttc cacttcggcg
     9181 gtaatgcctg ctccgacaac ggtttgcgca ttttgtgcag cgggaatctc caaaccgcgc
     9241 gcttgtgcgg cggagacgat ggcgcgggcg agcgggtggg cggcgttttg ctcgacggcg
     9301 gcggcgatgc ggtacaaagc gtcttcgtca aagccgctgt cgggaacgta ataaacggcg
     9361 gcaacctgcg gcctgccttc ggtcagcgta ccggttttgt ccaatacgac ggcatcgacg
     9421 tgggctgctt cctccattgc cgccgcgtct ttaaaccaaa tgccgtgttt caccgctttg
     9481 cccatgccga ccataatcgc ggcaggggtc gccagaccga gcgcgcacgg gcaggcaatt
     9541 accaaaacgg caacggcgtg catcagtgcg accgtccaat cgcccttaat cagccaagta
     9601 acgataaaag tcagaagcgc gatgcccacg acagttggca caaataccgc cgccgcttta
     9661 tcggccacgc gcgcaatcgg tgctttactg ccttgtgctt cagagagcgc gttcatcatg
     9721 tcgccgagca gggtttggct gccgagctgc gcggcgcggt acaccacgct gccttcggtc
     9781 atcagcgcgc ccgccaacac tttgccgccc gcctttttct cttcgggatt ggattcgccg
     9841 gtaaggtggc tttcgtccgc ccaaccgctg ccgctttcga taatgccgtc ggcagcgatg
     9901 cgttcgccgt ggttggtgcg gataaggtcg ccgatttgca cttggtcgat gggcagttgt
     9961 ttccattcgc cgttgcgttg cacgttgact tgggtcggcg tgagttttag cagtaagccc
    10021 aagctgttca ggctggattt tttggtgcgg tgttccaaaa acttacccag cgacacaaaa
    10081 ccgatcacca tcacgcccgc ttcaaaatac acatgcgcca taccgtgcgc cgcatgcgaa
    10141 ctgaaaaaca gcatataaac ggaatacagg taaatcgaca ccgtgccgat ggtaacgagt
    10201 acgtccatat tcgccagccc gcctttaatg cttgcccacg cgcttttgta aaacgggatt
    10261 gccagccaaa gttgcactat gcttgccagt acaaactgcc atacaggcgg aatcatccaa
    10321 tcgtgccgtg tccaattcag cccttttagc atcatcccta ccataccgat aaggaacggg
    10381 atattgatgg tcagcaaaag ccacaaccgc cagccgatat ggtgttctgc ttcaggttgc
    10441 ggcaatgtat cttccgtttt ttccttcgcg ccgtaaccgg ttttctcaat gattttggca
    10501 atgtcggcaa ccgaggtttt gctgccgtca aacgtaacct gcgcttcctc actggcaaag
    10561 ttcactcccg ccgattcgac aaaatctttt ttgttcaaca ctttttcaat gcgcgaagca
    10621 catgcctgac aggtcatccc ctcgatttgg aaacggattt tttgttgcat aagtttgatt
    10681 cccgttttga tgttcagacg gcagtcaagg ctgcgggatg ccgtctgaac gccggtatgg
    10741 tcaaaatttc tcaaacgcgc cgataattcg ggtagaatcc gaaaacttga cacagcaaag
    10801 gaaaaacaat gtcccaacac cgcaaactga ttattttggg ttccggcccc gccggataca
    10861 ccgccgccgt ctatgccgca cgtgccaatt taaaccccgt cattattaca ggtatcgcgc
    10921 aaggcgggca actgatgaca acgactgaag tggacaactg gcctgccgat gccgacggcg
    10981 tgcaagggcc ggaattgatg gcgcggtttc tcgcccacgc cgaacgtttc ggaacggaaa
    11041 tcatttttga ccaaatcaac gccgtcgatc tgcaaaaacg cccgttcgca ctcaaaggcg
    11101 atatgggcga gtacacttgc gatgccctga ttgtcgccac cggcgcgtcc gccaaatacc
    11161 ttggtttgcc gagtgaggaa gcgtttgcag ggaaaggcgt ttccgcctgc gccacctgcg
    11221 acggtttctt ctacaaaaac caagatgttg ccgtagtcgg cggcggcaat acggcagttg
    11281 aggaggcact ctaccttgcc aatatcgcca aaaccgttac gctgattcac cgccgcagcg
    11341 agttccgtgc tgaaaaaatc atgattgaca aactgatgaa acgcgtggaa gagggcaaaa
    11401 tcatcctcaa gctggaaagc aacctgcaag aagtactggg cgacgatcgg ggcgtaaatg
    11461 gcgcattatt aaaaaacaac gacggttctg atcaacaaat tgccgtcagc ggcatcttta
    11521 tcgccatcgg gcacaagccg aataccgata ttttcaaagg gcagttggaa atggacgaag
    11581 ccggctacct gaaaacaaaa ggcggcacgg cggacaatgt cggtgcaacc aatatcgaag
    11641 gcgtatgggc ggcgggcgac gtaaaagacc atacctaccg tcaggcaatt accagcgcgg
    11701 cctccggctg tcaggcggct ttggacgcgg aacgttggct gggcagccaa aacatttgaa
    11761 tgccgtctga aaccgaaatt ttcggcggaa tgcaagtttt tcgtaccaaa tccttgattc
    11821 cgtcggcaaa ccccaatata atgcctgcta tttgaacctt gccttctgct ttcgcatggc
    11881 agaaggctgt ttttaccatc cataaggaga taacatgcgt cattacgaga tcgtgtttat
    11941 cgttcatcct gatcaaagcg agcaagtgcc cgctatggtt gaacgttaca aaaccatgat
    12001 taccgaggca aacggtaaga ttcaccgttt ggaagattgg ggccgccgcc aactggctta
    12061 cccgattaac aaaatccata aagcacatta tgttttgatg aacatcgaaa ccactcccga
    12121 agttgttggg gagttggaaa ccgcgttccg tttcaatgat gcggtattgc gtcatctgac
    12181 catcaaaacc aaacacgccg ttaccgaagc ctcccctatg ctgggcggcg aaaaggctaa
    12241 gaacctgttg gtcggcgcgg ctgaagaagc ggctgcccaa taattgggat tcactaatct
    12301 tgtttcgctt gccgcgctga ttgaaaaggc tttccctatt cgatatacgc ctgccggaat
    12361 ccctgtttta gatattattt taaagcacga atcgtggcag gaggaaaatg ggcagcaatg
    12421 ccttgtccaa ttggaaatcc ccgcgcggat tttgggtagg caggcggaag agtggcagta
    12481 tcggcaaggt gtatatgttc acgtcgaagg ttttttagct caaaaaagca gacgttccct
    12541 gatgccgatg ctcaggatac aaaatattaa agaatataaa ggttaaacga caatggctcg
    12601 tcaatcattc aaacgtagaa aattctgccg tttcacggct gaaaaaatcc aagaagtcga
    12661 ttacaaacaa gttgatttgc tgaaagactt tatctctgaa aacggtaaaa tcattcctgc
    12721 ccgcatcaca ggaacgaagg cattctacca acgccaattg gctgttgccg taaaacgcgc
    12781 gcgcttcttg gcactcctgc cttataccga ccaacacaaa taattttgga gtttaaatca
    12841 tgcaaattat tctgttagaa aaaatcggcg gtttgggtaa tttgggcgac atcgttaccg
    12901 taaaaaacgg ttatgcccgc aacttcttga ttcctgctgg taaggcaaaa cgtgctactg
    12961 aagcaaacat gaaagagttt gaagcacgcc gcgccgaatt ggaagccaaa caggccgaaa
    13021 ttttggcaga tgcccgagcc cgtcaggaaa aactggacgg tcaaaccgtt accgttgcac
    13081 agaaagccgg tgtggacggt cgcctgttcg gttccgttac caatgccgac attgctgctg
    13141 caatcgttgc tgccggcatc gaagccgtga aagcaaatgt acgtctgccg aacggtcctc
    13201 tgaaagccgt tggagagtac gaagtggaag tggctttaca cactgatgcc gttgctaaaa
    13261 ttactgttgc cgtcattgcc gcagccgagt aattgaaatt atcagatgcc gtctgaaacc
    13321 ctgccgatta tcagaatgtg gtttcagacg gcatttgttt tgttttccgt atattctgca
    13381 aaaatcggga cggttgtttt ttatttgcct gaggtctttg cagaaatggt ttgttaacga
    13441 aatttgatgc ataaaatgcg ccaaaaaata ttggctgtgt tttaatatag tgttgacaag
    13501 cgtacctggt ttcaatatat tcaataggat aggtctcttc ccaaccgaaa aaataattgt
    13561 cttttctctt gtaaatagtt gaaatagatt gtcaaaatac atcctaaaaa atttaataac
    13621 ttatatgatt gcaaaggttg cggcttgtat gccggtcatt atggcggaaa taccgtttgg
    13681 gaggcagtcg catcgcatgg agtcggcacg gcaggcgatg cgccgatgcc ttcgggcgtg
    13741 aaggcaggga aatcggcagt ttggaaaaag gcaatggaat ccgcaggtag gtgtcgattg
    13801 ggtggtgaaa ggcaataagt cgggcgcacg ctcagactgc ctatcccctt gtcggaacgg
    13861 gaggtagggc agctttgtcc tgactaattg cttgtgcaaa cggtaagaag gtttttaaag
    13921 caggttgtac agcatttttc cggcaatcaa aagcaacata atgccgaagg attctttgag
    13981 tttggcagaa gaaagtttgt gggcggtttt gacaccgagc ggggcaaagg caatagttgc
    14041 cgcgctgagg acggcgacgg cgggcaggta aaggaagccc agcgaccctt cgggcaatcc
    14101 tgcaatattc agaccgttga ccagatacga tattgcgccg gagagtgcaa tcggccaggc
    14161 aaggccggat gatgtgccga tggctttatg ggcggggaag ccgcagtgga ttaagaaggg
    14221 gacggaaagt gaaccgccgc ctatgccgac ccagctcgac attgcgccga acagtgtgga
    14281 aaccgcagtc agtccgggca gcccgggcag cgggcgggat gccgtctgac gaccggtatg
    14341 cagtgttttg aatgcgacgg cggttaaaaa caggatgaag aaaatttgaa gcccgaacgc
    14401 ggggatatat tttgcggaga gtgcgccagc gaatacgccg aatatcatac ccggcatcat
    14461 cgcaaatatg gttttccagt cgaccgtctg ttttttgtgc tgccccaaca tactggaaaa
    14521 ggcggtgaag accatgacgg cgaaggatgt gccgacggcg aggtgttgcg cgtaaggatg
    14581 ttgtgccaaa ccctgcaaat caagcaccca taaaacgaca gggacaatca gcgtaccgcc
    14641 gcctacaccg aacaggccgg caataaaacc tgccgcactg cctacggcaa gcaggattaa
    14701 gataatgtcc caatgccaca tttaatccgc ctttctgtat tttttccaaa atcggcgtgc
    14761 cgccataatg tagccggaca ggctgtatcc gagaaagaac aggaaaagga cgagcgacgg
    14821 ttcccaagtg accagaagca gcagtaagac ggcaagcagc attccgacaa aggggacttg
    14881 tctgcggatg ttgatttctt taaaactcca aaaagggatt tggacaatca tcgacaggcc
    14941 ggcaaacagt gtgatgccta atgcccacca gtggacggcg gggaattttt cgacgctgtg
    15001 gttgacccaa atcagcccga caatcagcgc ggcggcagtc ggactgggta cgccgataaa
    15061 ccagcgtttg tccaccttgc cgatgagcgt gttgaacagc gcgaggcgta gggcggcgca
    15121 ggcgcagtag atgaaggcga cggaataacc gattttgccg aactgccaaa gctgccattt
    15181 gtaggcaata agcgcggggg caacgccgaa gctgaccatg tcggcaaggc tgtcgagctg
    15241 ctccccgaac gcgctttggc tgttggtcag ccgcgccacg cgcccgtcca taccgtccag
    15301 cagcatagag atgaataccg cgatggcggc ggtttcataa cgtccgtgca tggattgggt
    15361 aatggcgtaa aacgcggaaa acagcgcggc gatggtaaag gaattgggca gcaggtagat
    15421 actgttttga cgcagggagc gttgcggggt gttggtcggg gtttccatag ctgaagtgtc
    15481 tcacggacgt ggagaatcga taggggatta taacccaaac cattatcgaa caatacgccc
    15541 tttaggatga agccctcgga ggcggtttgt ttttctgatg gcggatgctt tgcggggcgg
    15601 tttgactctg cctgccaccg ggtgtgttgt ttcggatttt gccggatgga tattgttgcc
    15661 gggcatttct gcgcttgccg gaaggtatcg gagggaaggc atcggaatat gcggcgcgcc
    15721 gttcaaaccg gggcggtatt tccctatttt ttggggggtg caggtttcaa caaattgaaa
    15781 gatatttttt gcatttccga ccgtatgctg attttgaagc gggataaatt ataggacaag
    15841 gtttttcgcc cgccgcaaag gttgcccgca atgccgtctg aaaacaacgg cttgccgcta
    15901 aggcggcaat atgaggattt gcgctgccga aaccggtttg ctacaacccg gcagtgaaat
    15961 ctgcagggag ggataggccg ataaggcggg acttatcttg ttttttgttt ataatgcaac
    16021 agaatttgga ggaatcgtcg ggaatttatc atgatgaaaa agatactggc agtatcggca
    16081 ctatgcctga tgactgcggc ggcacaggct gccgatactt acggctatct cgccgtttgg
    16141 cagaatccgc aggatgcaaa cgatgttttg caggttaaaa ccacaaaaga agattcggcg
    16201 aaaagcgaag cgtttgccga gttggaagcc ttttgcaaag gtcaggacac gcttgcgggc
    16261 attgccgaag acgagccgac cggatgccgg tcggtcgtgt cgctgaacaa tacctgtgtc
    16321 tcgctggcat acccgaaagc cttgggcgcg atgcgcgttg aaaacgccgt cgtgattact
    16381 tctccgcgtt ttacgagcgt tcatcaggtc gcactcaacc agtgcataaa aaaatacggc
    16441 gcacagggac aatgcggctt ggaaacagtg tattgcacgt catcttctta ttacggcggg
    16501 gctgttcgct ccttaatcca acacctgaaa taaaaaatgc cgtctgaagg atgttcggac
    16561 ggcatttcca tatcgacggt caggattctt tcggatcggg cagcaggctg ttcaacacaa
    16621 tggcgagtac ggcgcacaag cccacgccga caaagctgaa gctgcccaat ttgagtgtca
    16681 taccgccgat gcccgtggtc agtaccgagc tgacgatgac caggtttttc ggcagcatca
    16741 aatcgacttt ggcatcaatc agggttttga tgcctaaaga agcaatcgtg ccgaacagca
    16801 gcagcataat gccgcccatc acgggcatcg gaatggaagc caaaaacgca ttgaatttgc
    16861 cgaaaaacgc catgcagacg gcaaaaaccg ccgcccaagt catgatgacg gggttgctgt
    16921 ttttggtaat catcaccgca cccgttactt cgccgtaggt tgtaaccggc gggccgccga
    16981 tcagacctgc aacgcacacg cccaagccgt cgcccgcaag ggttttgtcc aagcccgggt
    17041 cttttgtgta gtcttttccc gttacattgc cgattgccat gatgccgccg atgtgttcga
    17101 tggcgggggc gacggcaacg ggcagcataa acagtgccgc ctgccagttg acctgaggcg
    17161 tttcaaaatg gggaacggcg aaccagggcg cgtgtgcaat gcttgtcgtg tccaccagtc
    17221 ccatcagcag tgccaaaaca taacccgaag cgacaccgat caagatggga atcagcttca
    17281 tcatcctgct gccgaaaacc gatacgatgg cggtaacggc aaaggtaaag ccggaaagaa
    17341 tcagcgaatc ggtatagtcg atgacctgtt tgccgtcagc ctgacccatt gccatgctgc
    17401 ttgccgccac ggcaacagac agaccgatga ccatgatgac agggccggtg acgaccggcg
    17461 gcagcagttt gtgtaccgcc gccagtccgc gccaacggat cagcgcggca aacacaaaat
    17521 acataaagcc ggcggcaaac agcccgaaca tggtggaagg cagcccccat tcgccgacgg
    17581 agtagataat cggcgcgata aaggcaaacg aagaaccgag aaaaatcggt actttgcgtt
    17641 tggttgtgat ttggaacagc agcgttccca agcctgcgcc caaaagcgca agagccggat
    17701 tcagaccggt cagcagggga accagcacca ttgcgccgaa tgccacaaat aaaatctgcg
    17761 cgccggaaac ggcaagtttc agttggttca taccatcttc tttcaatcgg ttcaaacggc
    17821 acggattata ctggtttgcc gaggggctgc gtaaaaatac atacgtcggg caaagatatg
    17881 ccgtctgaaa ggcaacgtct gtctgttgca ggatgaaagc gggagaaatg gagtataatc
    17941 cgacactttc catattcaat ccgcaagccg tcccgaaccc ttatttcaga cggcattttc
    18001 cggtaatacg gcatgacaga aaaatcccat aaaaaaacag caaaaggcag ggcgggaagc
    18061 ccgtccccaa cgtccgcacg caacaagaaa gccgacaacg gcgcgcgggg caataaagtt
    18121 tccgagcggc tcaaggcggt caaagagctg cagaaaactg aagccaaaaa ggcgcgtccc
    18181 gaacatgtcg tcaacctgat tgccgacgcg ctgtggctga tgggtttggc ggcaaccctg
    18241 tatttggcga tttccctgat cagtttcgat atgggcgatc cgtcttggtc gcacagttca
    18301 ccggttgtgg aagatgccgc caattggggc ggactgttcg gcgcgtatgt tgccgatgtc
    18361 ggctattatc ttttcggctg gtcgttctgg tggtggatag cggctgcctg cgtcgtgctg
    18421 tataaaaatt tccgtctgca cgcaaaacag acggaaaacg aggcatacaa ccacaaaatc
    18481 gctgccgccg cgctgtttgt cctgacggtc ttcagccccg tcttggagta ttttgtgctg
    18541 ggcggaaaat atgccgactc cctgcctgtc ggagcaggcg gtatggtcgg catacgtgtc
    18601 ggcgcagtgt ttgcgtggct gttggggaaa tcgggcagcc tgctgattat cctggttgtt
    18661 ctgctgttgt cgttgtccct gctggtgcag atttcatggc tggaattttt gaacggtgcg
    18721 ggcagggcgg ttcaaaaccg cctgagtgcc ttatccggca aggtcatggc tttgagaaaa
    18781 cgccggccga ataccaaaac agacggtgtc gatacccaaa atacacggcg catggtgaaa
    18841 gaagccaaaa acatcactgc caaacccgtt gccttgcccg aaggcagcag cagcaaccgc
    18901 aaatccgtcg cggtttccgt tgcgccgccg cctaaaatcc aaccttcgct gtttgaagac
    18961 aatgaagtac agcagaacgg cgaataccac aagcctacat tgaacctatt gcggattccc
    19021 gacagcgagc cggtcagcat caatcccgcc gaattggagc gcactgccga actaatcgaa
    19081 tccaaactgg cagaattcgg catcggcgta caagtcgtat ccgcgacatc cggccccgtc
    19141 atcacgcgct acgaaatcga acccgcgcaa ggtgtcaaag gcagccaaat tgttgccctg
    19201 tcgaaagatt tggcacgctc tatgtcgctg caatccgtgc gtatcgtcga aaccattgcg
    19261 ggcaaaaaca cgatgggcat cgagttgccc aacgacaaac gccaagacgt gatgttgagc
    19321 gaaatcttgt cctcgcccgt gtttgccgaa gccaaatcca agctgaccgt cgcgctgggc
    19381 aaagacatcg ccggtacgcc cgtcgtcggc gacttggcga aaatgccgca ccttttggtc
    19441 gccggcatga ccggttcggg caaatccgtc ggcgtgaacg gcatgattat gtctatgctt
    19501 ttcaaagcta cgcccgaaga agtccgcttc attatgatag acccgaaaat gctcgagttg
    19561 agcatttacg acggcattcc gcacctactc tgtcccgtcg ttaccgatat gcgcgaagca
    19621 gggcaggcgt tgaactggtg cgtcgccgaa atggaaaaac gctaccgcct gctctcccac
    19681 gccggtgtac gcaatctgga aggcttcaac caaaaagtcg aacaagccaa agcggcaggc
    19741 aagccgctgc tcaatccgtt cagcctgaac ctcgacgaac ctgagccact ggaaaaactg
    19801 ccgatgatcg tggtcgttat cgacgaactt gccgacctga tgatgaccga acgcaaagct
    19861 gtcgagcagc aaatcgcccg cctcgcccaa aaagcgcgcg ccgccggcat acacatgatt
    19921 gtcgccaccc aacgcccaag cgtcgatgtc gtaaccggtc tgattaaagc caacatcccg
    19981 acgcgtatgg cgtttaccgt gcaaagcaaa atcgacagcc gtaccatcct cgaccaaatg
    20041 ggcgcggacg aactgctcaa atatggcgat tcgctgttcc tgcaaccggg cagtgccgaa
    20101 ccgacccgcc tgcaaggcac gtttgtttca gacggcgaag tgcatcaagt cgtcaactac
    20161 gtcaaatcgc aagccccagc cgactatatt gaaggtctgc tcagcggcga agccgcgctg
    20221 gaaactgcca atatcgttaa tccgaatgca gacagcgacg aattattcga tcaggcagtc
    20281 gcctatgttt tggaaagcaa aaaaacctcc atttcgtctt tgcagcggca gttgcgtatc
    20341 ggctataacc gcgcggcaaa cctgatggag gcactggaaa atgcgggtat cgtttctccc
    20401 tccgacctca acggcagccg taaaattttg gcgcacaagg accatttata gcccgtatcg
    20461 c
//
LOCUS       DAWTCI010000029        20331 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWTCI010000029 DAWTCI010000000
VERSION     DAWTCI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.029"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            53..322
                     /locus_tag="WM481_001064"
     CDS             53..322
                     /locus_tag="WM481_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080393.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGILPKPVALSIVQYEND
                     PGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            304..456
                     /locus_tag="WM481_001065"
     CDS             304..456
                     /locus_tag="WM481_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080394.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            443..988
                     /locus_tag="WM481_001066"
     CDS             443..988
                     /locus_tag="WM481_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9080395.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMKRKPFAW"
     gene            1392..1793
                     /locus_tag="WM481_001067"
     CDS             1392..1793
                     /locus_tag="WM481_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080396.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKTENNSDGNPTANYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(1936..2043)
                     /locus_tag="WM481_001068"
                     /pseudo
     CDS             complement(1936..2043)
                     /locus_tag="WM481_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2065..2559
                     /locus_tag="WM481_001069"
     CDS             2065..2559
                     /locus_tag="WM481_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080397.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKDLFAEEVPENNIVCDISHFLHFADRA
                     EADIPNQSRLYAEYWWEMETFKMSVLKDWERSGRPLDWEKWQRQYQSEAFELVKNLNR
                     SKQE"
     gene            2679..3812
                     /locus_tag="WM481_001070"
     CDS             2679..3812
                     /locus_tag="WM481_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080398.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNTFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRGLSH"
     gene            complement(4315..4590)
                     /locus_tag="WM481_001071"
     CDS             complement(4315..4590)
                     /locus_tag="WM481_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9080399.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAIHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRQLWEQGEKMPSET"
     gene            complement(4587..5870)
                     /locus_tag="WM481_001072"
     CDS             complement(4587..5870)
                     /locus_tag="WM481_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9080400.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAHEPVLPPSVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAITVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWMG
                     YVPFFRLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(6019..7437)
                     /gene="glnA"
                     /locus_tag="WM481_001073"
     CDS             complement(6019..7437)
                     /gene="glnA"
                     /locus_tag="WM481_001073"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFA9080401.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGPAFDGSSIGGWKGIEASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(7438..7653)
                     /locus_tag="WM481_001074"
                     /pseudo
     CDS             complement(7438..7653)
                     /locus_tag="WM481_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
     gene            7748..8557
                     /gene="aroE"
                     /locus_tag="WM481_001075"
     CDS             7748..8557
                     /gene="aroE"
                     /locus_tag="WM481_001075"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFA9080402.1"
                     /translation="MHALPRYAVFGNPAAHSKSPQIHQQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLVWLEDGRIRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSPKIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFKPDIRPVIEHMKAL"
     gene            8560..9261
                     /gene="mtgA"
                     /locus_tag="WM481_001076"
     CDS             8560..9261
                     /gene="mtgA"
                     /locus_tag="WM481_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFA9080403.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWMPYKRISTNLKKALIASEDVRFAGHGGFDWGGIQNAIRRNR
                     NSGEVKAGGSTISQQLAKNLFLNESRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSI
                     EWHYGVFGAEAASRYFYKKPAADLTKQQAAKLTALVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(9535..9610)
                     /locus_tag="WM481_001077"
     tRNA            complement(9535..9610)
                     /locus_tag="WM481_001077"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9575..9577),aa:Phe,seq:gaa)
     gene            complement(9801..10535)
                     /gene="lptB"
                     /locus_tag="WM481_001078"
     CDS             complement(9801..10535)
                     /gene="lptB"
                     /locus_tag="WM481_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFA9080404.1"
                     /translation="MSANNSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTGFYMIVGLIAADAGSVMLDGRELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRNPAPSLSGGERRRVEIARVLAMKPR
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(10606..11136)
                     /gene="lptA"
                     /locus_tag="WM481_001079"
     CDS             complement(10606..11136)
                     /gene="lptA"
                     /locus_tag="WM481_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFA9080405.1"
                     /translation="MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAN
                     QSTTFSGNVIIRQGTLNISASRVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVTYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKSASKT
                     GRVSVVIQPSSTQKTE"
     gene            complement(11117..11698)
                     /gene="lptC"
                     /locus_tag="WM481_001080"
     CDS             complement(11117..11698)
                     /gene="lptC"
                     /locus_tag="WM481_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFA9080406.1"
                     /translation="MKVRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPENSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYNHKTGMLNFSSKVKAAIYDTKDM"
     gene            complement(11695..12231)
                     /locus_tag="WM481_001081"
     CDS             complement(11695..12231)
                     /locus_tag="WM481_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFA9080407.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDDGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQA
                     GLEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIIRAKGTLGAALNGYIK"
     gene            complement(12297..13271)
                     /locus_tag="WM481_001082"
     CDS             complement(12297..13271)
                     /locus_tag="WM481_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFA9080408.1"
                     /translation="MAENEKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVAAISNSG
                     ESDEITAIIPALKRKDITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKGAIVSMSEKGLGMLAVTDGQGCLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLTGALNMHDLLAARIV"
     gene            13368..14423
                     /gene="tal"
                     /locus_tag="WM481_001083"
     CDS             13368..14423
                     /gene="tal"
                     /locus_tag="WM481_001083"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFA9080409.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKRQNLSPKQRYETMAVADVRAACDVCLAEHESTGGKTG
                     FVSLEVSPELAKDAQGTVEEARRLHAAIARKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIQVVASFFISRVDSALDATLPDRLK
                     GKTAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGVHTVNTVPDATLKAFIDHGTAKATLTESADEARARLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            14574..14864
                     /locus_tag="WM481_001084"
     CDS             14574..14864
                     /locus_tag="WM481_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080410.1"
                     /translation="MAKALEIISPDEIYSDLIFKDPVPPHTIYTELMKLVGRNAGNER
                     IADRAFDFFSPALMDDSATEEQYNALYDLTLLEEPGMELDKDEITALINSLK"
     gene            14881..15768
                     /gene="gluQRS"
                     /locus_tag="WM481_001085"
     CDS             14881..15768
                     /gene="gluQRS"
                     /locus_tag="WM481_001085"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFA9080411.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVTYQSRRYALYEETLYRLKTAGLVYPCHCNRK
                     DWQAGARRGTDGFVYNGRCRHPGQRPALQGKQPSWRIRVPDRDIGFSDGIVGSYTQNL
                     ARDIGDFVLFRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDSNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMDKVPKHAITAP"
     gene            complement(15838..16854)
                     /gene="dusA"
                     /locus_tag="WM481_001086"
     CDS             complement(15838..16854)
                     /gene="dusA"
                     /locus_tag="WM481_001086"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFA9080412.1"
                     /translation="MINKELIEHKGKRRLCVAPMLDWTDRHYRYLARQITRNAWLYSE
                     MVNAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGC
                     PSPRVQKGAFGACLMNEVGLVADCLNAMHDAVGIPVTVKHRIGVDRQTEYQTVADFVG
                     TLRDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPELEIIINGGIT
                     TNEAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDNRAPIEYADLVQRLYTYSQV
                     QIQAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGGLIFEAWKEVERA
                     NTRE"
     gene            16974..18128
                     /locus_tag="WM481_001087"
     CDS             16974..18128
                     /locus_tag="WM481_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFA9080413.1"
                     /translation="MATITKRRNPSGETVYRVQVRVGKKGYPAFNESRTFSKKALAVE
                     WGKKREAEIEAGPELLFKRGKVKMMTLSEAMRKYLNETLGAGRSKKMGLRFLMEFPIG
                     GIGIDKLKRSDFAEHVMQRRRGIPELDIAPIAASTALQELQYIRSVLKHAFYVWGLEI
                     GWQELDFAANGLKRSNMVAKSAIRDRLPTTEELQTLTTYFLRQWQSRKSSIPMHLIMW
                     LAIYTSRRQDEICRLLFDDWHKNDCTRPVRDLKNPNGSTGNNKEFDILPMALPVIDEL
                     PEESVRKRMLANKGIADSLVPCNGKSVSAAWTRACKVLRIKDLRFHDLRHEAATRMAE
                     DGFTIPQMQRVTLHDGWNSLQRYVSVRKRSTRLDFKEAMMQAQSDIKSGK"
     gene            complement(18178..18444)
                     /locus_tag="WM481_001088"
     CDS             complement(18178..18444)
                     /locus_tag="WM481_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFA9080414.1"
                     /translation="MNISKEQGLLIAYGGRPYIPPREVHKDFFAHIGFNAFKASGVRC
                     ELPFPIFRLSDSRKSEYFVSVRELAKAIADKEEKAKSEYRKFRS"
     gene            complement(18552..19430)
                     /locus_tag="WM481_001089"
     CDS             complement(18552..19430)
                     /locus_tag="WM481_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFA9080415.1"
                     /translation="MRRLVAAKVRRYCGEHPGVFDGAAGSGQLEQYIEPSDFRAVEIQ
                     AEACKALLQNYPAAKVYNTSLFLYTDGEPQDCTVMNPPFSIKLKDLSEDEKSRIAQEY
                     PWKKSGVADEIFVLKGLENARRFGFFILFPGIAYRKSEQRFREIIGNRLAELNRIQNA
                     FEDTPIEVLLLVIDKDKTDGGCIRELYDCKTDTLLAADTWQIEPDLWQTVQEPAPPKE
                     KEDPVLLEHECRDAAAKRIARELRFSKMVNEIEGWPHAEFDGFCDRLCNLIQAEKYGK
                     KHYFPCSLPLFGGAAG"
     gene            complement(19471..19701)
                     /locus_tag="WM481_001090"
     CDS             complement(19471..19701)
                     /locus_tag="WM481_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080416.1"
                     /translation="MRTHIRTCVYHDSGTKGFKHGIRHKRHGCRRGGTSVFQRGEDGK
                     PQRVSRIRKRSATPGQARERTKTHDVSRTQQP"
     gene            complement(19698..>20331)
                     /locus_tag="WM481_001091"
     CDS             complement(19698..>20331)
                     /locus_tag="WM481_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080417.1"
                     /translation="ESVLFACREASQAVAQWTKDFDDLSPNQRQLVLRPLSNLFAAYE
                     EFLKDAPARLIAEVSAYSLAVRVAKKAMAFLELDGGLISAVGKVVNGADSRAEARRLK
                     MPYAEFTGRILHAANLLYDVGIQADKELSAMYGRPLNPVRPRRISDVRRPMMKMLVAD
                     KGGALVRAVKDSEDVIRHCDNGAGFSCFNWTEHFKRTANLISLMHREAAA"
BASE COUNT         4820 a         5241 c         5379 g         4891 t
ORIGIN      
        1 tggacgtgta atagaaaata gtgtagtaac aattattcag gattagaaaa ttatgagtgt
       61 aaaagaatta ttcaaagtta tcttagataa aaataaagat tttcctatta gaacgattca
      121 tcgcactcca atggggatac ttcccaaacc tgttgcttta agtattgttc aatatgaaaa
      181 tgacccggga ttttatttgt tttatttgga tgagaccggt caggaacaaa cggatactta
      241 tcatgacaca ttagattcgg catttgaaca ggctgaattt gaatttggaa tcagcaaaga
      301 ggaatggatg caaagtcctt agacggcaaa atcaagatgc cgctttattt ttcagatggc
      361 atttccgtca ataaaaaagt cggtattgga gcccaaaaca gcctacgtct tgccgggata
      421 gggatgaaat ctttaagaaa aaatgattat agttgaacat ttagaacatt atttgggtga
      481 aatcgaaagc ggtataaaat gcttggatcg acgttatcat ctcagcgttt ccgtcttccc
      541 atcacaacca tataaagggg taactacttt ttctacttta ggcttgaatc gatatgactt
      601 gaactataag agtaggtttg aattaatttt tacctgttct gaagaatgga ataaagaaaa
      661 tattgcagct tttctatcag gagtggctga atatttgatt gacaatagac aacctatttt
      721 aagaggtgaa attattcaat tacccagggt aattatcgaa ggtagcaaaa tggatgcact
      781 gtatgtttcc gccccatttt atttcgatga tgattttcag gtctgttatg gcgaacacta
      841 caatatcgtt ttccctttgc ttgtcccctt gtataaacag gaagcagaat tggtggaaaa
      901 gaaaggttgg aatgcttttg agcagttctt gctggataat gaagttgaca acctttcgga
      961 tatgaagcgg aaaccgtttg cttggtaagg gctttcttaa atttgattaa aggtcgtctg
     1021 aaattaagct tgtttttcag atggtctttc cttcatttga agcaggatat tgagaactga
     1081 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1141 ggctgaaaag caaacaccgt ccgtcgtgtt gccgtttgcg gatgagtacg ggtcaacccc
     1201 aatgccgccg aaaccgtcga agccgccttc aacattgcca ccgccaaagc cgcgaagttg
     1261 gcaaaaacgg taaaaccggg gagataaaag ccgatggcag gaaagtaaat gtgaggatag
     1321 acagtacgga ggcagacctg ctttatccgg cagggcaata agaaaacaaa acttagatat
     1381 ggaaaacgat tgtgaagatt aaaccattac aattttctaa caataatcac agattttatg
     1441 tggacaaaat ttgtgttacc gaacaagatg tgaacatttt ggcatccgac cgaaattatg
     1501 aattaaatat tgaaatattt attgacaata taattcattt tcaaataacg gatgaatctt
     1561 ataaagtaaa attttcagaa tatttattcg aaaataaaac agaaaataat tcggatggga
     1621 atcctactgc aaattatttt ttcgagataa tagatgacag ttatatggac tggttgaaag
     1681 aagaaagttt tggttttttt gaaaagaaat attataaggc ttatattttc ttttttagcg
     1741 attctgtaat agaagttatc agctcgacag aacctgtatt ttattcaaaa taacaaatta
     1801 tcaaacaaaa ctctgattaa aaacccaaca atcagaatgc cgtctgaagc ggtattcggc
     1861 tttcagacgg cattttctcc gattggcaca ggatgcaggg gcttgccggc ttgattttgc
     1921 cgaagttgtt ggctcgtata gtggattaaa tttaaaccag tacagcgttg cctcgccttg
     1981 ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttaaat ttaatccact
     2041 ataatttgga agaggcagca acctatggaa aatagatttg aaaaaatcgt taaggatatt
     2101 tttagtttta cccaatttgt ttccgaagcg gaaaataaaa ttcctaacca atcctcccgt
     2161 tattacgact gctggtggga attggaaatc ctgaatgcct ccgcattgga agattgggaa
     2221 atatcgggaa agcctgaaaa atgggagaaa tggacagaaa aatatcagac cgaagcgtta
     2281 cagtcagtta aggatctatt tgccgaagaa gttcctgaaa ataatatagt ttgcgatatt
     2341 tctcattttt tgcattttgc cgatcgggca gaagccgaca ttcccaatca atcacgttta
     2401 tatgccgaat attggtggga aatggaaacg ttcaaaatgt cggttttaaa agattgggag
     2461 cgttcaggca gaccactaga ttgggaaaaa tggcaaaggc aatatcaatc cgaagcattt
     2521 gaattagtca aaaatttgaa ccgaagcaaa caagagtaac aagccgcagc ctgcataggg
     2581 gtttttaaag tacggcagca accgtagatt agattaaaaa tcaaacaatc aaagcccgcc
     2641 tgatataatc gatatatcct gttaaacgaa aaataaatat gtttaaaaaa ttcaaaccgg
     2701 tattgttgtc attttttgca cttgtatttg ccttttggct gggaacgggc attgcctatg
     2761 agattaatcc gcgttggttt ttgagcgata cggcaactga aatacctgaa aatccgaata
     2821 cttttgtggc gaaacttgcc cgcctgttcc gaaatgccga cagggcggtt gtcatcgtga
     2881 aggaatcgat gaggacggag gaaagccttg ccggagctgt ggatgacggt ccgttgcagt
     2941 cggagaagga ttatctcgcg ctcgctgtcc ggctcagtcg tttgaaagaa aaggcgaaat
     3001 ggtttcacgt aacggagcag gaacatgggg aagaggtctg gctggattac tatatcggcg
     3061 agggcggttt ggttgcggtt tcgcttttgc aacgctcgcc ggaagcgttt gttaatgccg
     3121 aatatctgta tcggaacgat cgtccgtttt ctgtaaatgt gtacggcgga acggctcacg
     3181 gggaaaatta tgaaacgaca ggagaatatc gggttgtttg gcaaccggac ggttcggtat
     3241 ttgatgcgtc ggggcgcggg aaaatcgggg aagatgttta tgagcattgc ctcgggtgtt
     3301 atcagatggc ccaggtatat ttggcgaaat accgggatgt cgcgaatgat gagcagaagg
     3361 tttgggactt ccgcgaagag agcaaccgga ttgcatcgga ctcgcgcgat tctgtgtttt
     3421 atcagaatat gcgggaattg atgccccggg ggatgaaggc gaacagtctt gtggtcggct
     3481 atgatgcgga cggtctgccg caaaaagtct attggagttt cgacaatgga aaaaaacgcc
     3541 agagtttcga atattatttg aaaaacggaa atctttttat tgcacaatct tcgacggtag
     3601 cattgaaagc ggatggcgta acggcggata tgcagaccta tcatgcgcaa cagacgtggt
     3661 atttggatgg cgggcggatt atccgcgaag agaaacaggg agacagactg cctgattttc
     3721 ctttgaactt ggaagatttg gaaaaagagg tgagccgtta tgcagaggct gcggcgagac
     3781 gttcgggcgg caggcgcggc ctttctcact gaacgcaaat accgtctgaa accggttttc
     3841 agacggcatt ggagagtcaa acgctttttg caatgccgta tatgccgaac ccggttcgct
     3901 acaaattgaa aaccacaatc caaaaaacgg aaatacacat tatcaaacaa acaaataagt
     3961 tgacatattg tgcgatattg agggctgtac cgtattttcc cgttctttat atgagcggca
     4021 ggcatcctca taaaagaagg gataccgata aggaaaaatc cctcggtata aaaaatatac
     4081 tgattaatca atacgaatcc aacaaacagt tcatcatttt cttttctgtt ggcgtataaa
     4141 aaccgctcgg aaaaaaataa aatatcaaaa cggcaaaata aaaatactga tatatgcctt
     4201 cactttcata gggcgacgct ctatgtgccg tcctgtgtgt tgaaacgtcg gttttcctta
     4261 ccgcctgccc cgcttttccc tgattgctgc caactgccgt ccgaacctga accgtcaggt
     4321 ttcagacggc attttttctc cctgttccca caattgccgt ttttgtgcgg cggtcagttt
     4381 tttgacggcg atttcctgtt tgagcgcagt gcctttatcc atgccgcctg caacgatacg
     4441 catcgccacc ggtttgaata cgcgggtata tttcgcgcct tttccggctg tgtggatggc
     4501 aagccgctgt tgcggattcg ggctgatacc gcaatagaac gcgctgtttt cacacaatat
     4561 caggtaaacg ctccagtttg acgcgttcat ctgcctgcat cctgggtttt ttccccgttc
     4621 caaggcgcaa ctttcagcag cagcagcata cccggcaggg caagtatgaa acacagccgg
     4681 aaaaacggta cataacccat ccattcgatc agatagcctg caaaggaatt gatgaccgtg
     4741 cgcggcacgg cggacaggct ggtaaacagc gcaagctgcg ttgccgtaaa cgcgggattg
     4801 gtctcgcgcg ccatatacga tacaaacgcc gccgtcccca accccacgcc gaccgcttcc
     4861 gcgccgataa ctgccgccag catcagcctc tcgcctgtgc cgaccgtgtc gaaatgtccg
     4921 aaccctgcca gccatacaaa ccccaaaacc gttattgcct gcaccgcgcc gaatagccac
     4981 aaggctttgt ttacgccgat tttcagcatc cacacgccgc ccaagatacc tgccgccact
     5041 gccggccaca gtcctgcatt tttggcaatc aaaccgatgt cggtcttgct gaaacccata
     5101 tccagataaa acggcgttgc caacgcggtt gccatactgt cgccgagttt gtaaaggaag
     5161 ataaacagca gcacgcacac cgccgaagcg atgcccttgc gcgtaaaaaa ttctttaaac
     5221 ggctctacca cggtctgctt caacgtttta ggaacggaag gcggcaacac gggttcgtgc
     5281 gcgagaaaca gcgtcatcag aaggccgggc agcataaata atgaagtgat aacaaatact
     5341 tctgaccacg gcatcctgtc tgccaacacc aaactcaatg aaccgggaat cagggcggca
     5401 acccggtagg cgttcacatg aaccgagttg cccaaaccca attcttcgtc cgacaaaatc
     5461 tcgcgcctga acgcatccaa tacaatgtcc tgactggcgg aaaaaaaagc gacaagcacc
     5521 gacaggccgg caatcaacgg cagatgattc cgggggttta aaaaggcata tgccgccaaa
     5581 gccgccagta accctgcctg cgtcagcagc atccacccgc gccgccgtcc caaaacgggc
     5641 agcctgaccg cgtccatcag cggcgaccac aaaaatttcc aagtaaacgg cagaccgatc
     5701 aacgccatca gcccgatgct cttcaaatcc acctgctcgc tgcgcaacca cgccggaatc
     5761 aggttaatca gaaagtacag cggcagcccc gaggcaaacc ccgtaaaaat acagacgagc
     5821 atattacggg aaaagatttg cccgataaaa cctgatttcg atgcagtcat cgttcgtaca
     5881 gccaaaaaac aaaagcctat tgtagcaaat atcgcccgcc tgctgaaaaa tgccgccacg
     5941 cgccgccggt ttgccgttcc aatgcaaaat gccgtctgaa actgtgttca gacggcattg
     6001 ctactgaaac ccgacggttt acaggctgta atacatttca aattccagcg ggtgcggcgc
     6061 catacggatg cggcggacat cttcctcttt aaaggcgatg tagctgtcga tccagtcttt
     6121 gctgaacacg ccgccgcgca gcaggaattc gtggtcggcc ttgagggcgg caagtgcttc
     6181 ttccaaagaa gcgcagacgg tcgggacgag cgcgtcttct tccggcggca ggtcgtacag
     6241 gtttttatcg gcagggtcgc ccggatggat tttgttttga atgccgtcca aaccggccat
     6301 cagcagggcg gcaaatgcca aatacgggtt ggcggtcggg tcggggaaac gcgcttcgat
     6361 gcggcgcgcc ttgctgctgt tcacagacgg gatacggatg gaagcggaac ggtttttggc
     6421 ggaataggcc aatttggtcg gtgcttcaaa gtgcggcacg aggcgtttgt aggagttggt
     6481 ggacggattg gtaatcgcgt tcagggcttt ggcgtgtttg atgatgccgc cgatgtagta
     6541 gagcgcggta tcggacaaac cggcatagcc gtcgcctgcg aacaggtttt gaccgtcttt
     6601 ccaaatggat tggtggacgt gcataccgct gccgttgtcg cccataatcg gtttgggcat
     6661 aaaggtggcg gttttgccga aattgtgggc aacgttttgg atgacgtatt tcatatcttg
     6721 ggtttggtcg gcgcgtttga ccaaagtggc gaaacgggtg ccgatttcca tttggctgcc
     6781 ggtaccgact tcgctgtggt ggacttcgac ttcgatgccg agtccttcca aaatgttcac
     6841 catggcggag cgcaaatctt gaccgcagtc aatcggcgcg acgggcgcgt agccgccttt
     6901 gacggcgggg cggtggccgg tgttttgacc gtccatatgc aggccgcttg cccacgcgcc
     6961 gctttcggac gtgatttcgt aacgggtttt gtgcatgtcg gtttcaaatt ctacgccgtc
     7021 gaagacgaag aattcgggtt cggggccgaa ataggcggtg tcgccgatac cggaagattt
     7081 caaataggct tcggcgcggc gtgcgatgga gcgcgggtcg cggtcgtaac cctgaccgtc
     7141 ggcagggtcg atgacgtcgc aggtaatgac gacggtaaca tcatcataaa aaggatcgac
     7201 gaaggctgtg gacgcatcgg gacgcagctg catatcggaa gcctcaatgc ctttccagcc
     7261 gccgatggac gagccgtcaa acgccggtcc gttttcaaac cactcttcgg ggtcttccaa
     7321 cacgatgcgc gcaggcacgg taaagtggtg ctgcttgcct ttggtatcgg tgaagcgcaa
     7381 atcgacaaag cgggcttcgc tttcttcaat caattttacg gcgtttttaa tggacatctt
     7441 cagctcctga aaaagggaaa tggcaaaata tgcggaacgc ggcggcttga cgcaaagtcg
     7501 gcacgatttt accacgccga tgccgtctga aaccggtttt gcgtcaaatt ccaagccgga
     7561 aaatatagtt tcaaacggca agattgtcaa caatatgctt cggtttgtct gttcaaaagc
     7621 ggaatgcccg cccttttgtc cgaatgccga caaaacagat gccgtctgaa ggcggtttca
     7681 gacggcatcg ttcccatctt cgcgccgccg ttataaaatg accgccccgc ctttatggaa
     7741 tatgactatg cacgccctcc cccgctacgc cgttttcggc aaccccgccg cccacagcaa
     7801 atcgccgcag attcatcagc aatttgccct tcaggaaggc gttgacatcg aatacggacg
     7861 catttgcgcc gacatcggcg gtttcgcgca ggcggtttcg acattttttg aaacaggcgg
     7921 ttgcggggca aacgttaccg taccgttcaa acaggaagcg tttcatctgg cggacgaaca
     7981 ttccgaccgc gctttggctg ccggtgcggt caatacgctg gtgtggttgg aagacggcag
     8041 aatacgcggc gacaataccg acggtatcgg tttggccaac gacatcacgc aggtcaaaaa
     8101 cattgccatc gagggcaaaa ccatcttgct tttgggcgcg ggcggcgcgg tgcgcggcgt
     8161 gattccggtt ttgaaagaac accgccctgc ccgtatcgtc attgccaacc gtacccgcgc
     8221 caaagccgag gaattggcgc ggcttttcgg cattgaagcc gtcccgatgg cggatgtgaa
     8281 cggcggtttc gatatcatca tcaacggcac gtccggcggc ttgagcggtc agcttcctgc
     8341 cgtcagccct aaaattttcc gcgactgccg ccttgcctac gatatggttt acggcgaagc
     8401 ggcaaaaccg tttttagatt ttgcacgaca atcgggtgcg aaaaaaactg ccgacggact
     8461 gggtatgcta gtcggtcagg cggcggcttc ctacgccctc tggcgcggtt tcaaaccgga
     8521 catccgcccc gtcatcgaac acatgaaagc cctgtaacca tgttccgcat cgtcaaatgg
     8581 ctgattgccc tgcccgtcgg catctttatc ttcttcaatg cctatgtgta cggcaacatc
     8641 attacctacc gcgccgtcgc gccccatcgg actgccttta tgtcgatgcg gatgaagcag
     8701 tttgaacaag aaggtcgcga tgtcgcactg gattaccgct ggatgcccta caaacgcatt
     8761 tccaccaacc tgaaaaaagc cctgattgct tccgaagatg tccgttttgc cggacacggc
     8821 ggcttcgatt ggggcggcat tcaaaacgcc atcaggcgca accggaacag cggcgaagtg
     8881 aaggcgggcg gatcgaccat cagccagcag cttgccaaaa acctcttcct caacgaaagc
     8941 cgcaactatc tgcgcaaagg ggaagaggcg gccattacgg caatgatgga agctgttacc
     9001 gacaaaaaca ggattttcga actgtattta aactcaatcg aatggcacta cggcgttttc
     9061 ggcgcggaag ctgcgtcccg gtatttttat aaaaaaccgg ccgcagacct gaccaaacag
     9121 caggcggcga aactgacggc actcgtcccc gccccgctct actacgctga ccatccgaaa
     9181 agcaaacggc tgcgcaacaa aaccaatatc gtgctcagac gcatgggttc ggcagaatta
     9241 cccgaaagcg atacggactg attgtttcag atatggaaat gccgtctgaa ctgcggttcg
     9301 gacggcatat gttttctggt tcttatagtg gattaaattt aaaccggtac ggcgttaccc
     9361 cgccttgccg tactatgttg tactgtctgc ggcttcgtcg ctttgtcctg atttttgtta
     9421 atccactaac ctgcccgaca gccgcaaaga ccatgtcgtg catggcaggt atttgcggtg
     9481 cgggactgct gccgaaaacg gcaggcaaaa aataaccgct tataagcggt tttttggtgg
     9541 ccagaggcgg aatcgaaccg ccgacacacg gattttcaat ccgttgctct accaactgag
     9601 ctatctggcc tgtatctgtc tcgttaagag gtgcgtatta aaccaaattt cgggacgaca
     9661 cgcaagcgga tttgtcgata ttttttaccc aaccgactaa gtcgatgttt taagtttaaa
     9721 ttaatttcac agaaaaatag tgcaaatcgg ttttttgccg ttcgttcggc gatgttctgc
     9781 tttgcagacg gcaaaacgct tcaatatttg aagttctcgc ccaaatagac ggcgcgcacc
     9841 tgttcgttgc cgaccaaatc gtcgggcttg ccggaagcca gcaccgcacc gtctgaaatg
     9901 atgtaggcgc ggtcgcagat gctgagggtc tcgcggacgt tgtggtcggt aatcaatacg
     9961 ccgatgccgc gcgatttgag gaaaccgatg attttctgga tgtcgatgac ggcgatggga
    10021 tcgacgccgg caaaaggttc gtccaaaagg atgaaacgtg gtttcatggc gaggacacgg
    10081 gcgatttcga cgcgccgccg ttcgccgccg gacagcgacg gcgcaggatt gcggcgcaag
    10141 tgtccgatat tgaggtcggc gagcagtttt tcgatttctc tgtcgatttg atttttatct
    10201 ttggttctga tttccaagat ggcgcggatg ttttgttcga cggtcatttt gcggaatatc
    10261 gaggcttcct gcggcaggta gccgacaccc aggcgggcgc gttcgtgtat gggcaggtgg
    10321 cgcaattccc gcccgtccag catcacgctg cctgcgtcgg cagcgatgag cccgactatc
    10381 atgtagaagc cggtggtttt gcctgcgccg ttgggcccga gcagtccgat gacttcgccg
    10441 ctttcgatct caagggagaa acttttgacg acttggcgtt ttttgaaact tttctgcagg
    10501 ttttggacga cgaggcggct gttgtttgcg ctcataaatc tcttctcggc atttcggtcg
    10561 gcaaatgccg tctgaactgc gtttccgttt cagacggcat cggggttatt cggttttttg
    10621 tgtgcttgaa ggctggatga cgacgctgac cctgccggtt ttggaagcgg atttcgcacc
    10681 cgatttcgtg ctgccgttga tggtatagac ttcggttttg gtgttgtagg taatgaccgc
    10741 accttcggca acgtcgccgc cgcgctgcac tttggcattg ccggtcagaa cgacggtgct
    10801 tcctgcggag gaataggtaa cgttgtttgc ctgaccgcgc accgtccctt tgcccccgtc
    10861 caacgtttgg ctgaagcgga cgggcgaacc ttccgccctc acggattcgc cgcctttgcc
    10921 gccgcgtgtg acgttgacgc gcgaggcgga aatgttgagc gtaccctgtc tgatgatgac
    10981 attgccgcta aatgtggtac tttggttggc ttgatcgagc gaaccttggt cggcttcgat
    11041 ttggatgggc cgtctgctgt cgctttgaag ggcaaaagcg ggagaagttg caaaaattac
    11101 aattaaaaca aatagcttac atatcttttg tatcataaat cgcggctttc actttagatg
    11161 agaagttcaa catgcctgtt ttgtggttgt aggtcatacc gcccgcctga ccgtgcgacg
    11221 cgccatattg gaaactgaca ggcgtatcgg tttgggcata ttgagattcg gtatcgacgt
    11281 gcagtttttc ggtttcgact ttacccgcct gccgcctgcc gtcggcggtt ttggtcagca
    11341 caacgttgtt tttaaaaaga acctgtttgt tttcggtatg gtaaacggct tcatcgctgc
    11401 cgacttcgta caacagcctg ccttcttgga agaacacgag atgcggcgaa tcaaaatgga
    11461 tgtcgctgtt ttcgggaaac tgtttcgcac ctttcgcgct caaatgttct ttcaagtatc
    11521 cctgttcgtc aaaccgcctt ccgtccaagc cgtccattgt gtattgaggt tcgtcgggat
    11581 tgagcctgac ttcctcgatt tcgacttcgc tgatacggcc caaccatgcc gacaggccgc
    11641 ccaaggcaac cgccaatatc aatgggaacg caattccgta ccgccatctt actttcattt
    11701 gatgtacccg ttcaaagccg cgcccaacgt acctttcgcg cggataatca ggtcacacac
    11761 ttcgcgcacc gcgcccgcac cgccggggcg tttggcaata taggcggcgt gttgcagcgt
    11821 aaaccaatgc gcgtcgggga cggcaaccgg caatccgcag cgcaccatca ccggcaaatc
    11881 gaccacgtcg tcgccgacaa aggcgcactc ggcttcttcc aagcctgcct gcgcgcgcag
    11941 ctcttcatac gcggcgcgtt tgtccgaaat gcctttgaag tagtaattta tgcccaactg
    12001 tttgacgcgg atgccgacgg agggcgcgtc ccgacctgtg ataatcgcag tctgcacgcc
    12061 gcttgcctga agcattttca ggccgtgtcc gtccagtgtg tgaaacgatt tgatttcttc
    12121 gccgtcgtcg cggataaaga tgcgcccgtc ggtcaaaacg ccgtccacat ccaatatcag
    12181 cagtttgatt ttggcggcgc gtgcctgtaa ttcgggagaa attgcctgca ttgggatttc
    12241 cttgttttca gacggcatat cttaccacaa atgccgtctg aaacggctgc aacgccttat
    12301 acaatccgcg ccgccaaaag gtcgtgcata ttcagcgcac ctgtcagcac gccgtctgca
    12361 tcggtaacca gaagcccgtt cacatggttt gcctgcatga ctttcagggc ttcggtggcg
    12421 aggcgttcgg cggagatggt tttagggtgt gtatgcatga cttcgtctat cgaaagaccg
    12481 gtaaaattgt cgcattcttg aaacaggcgg cgcaaatcgc cgtcagtaaa cacgcctttc
    12541 agacagcctt gcccgtccgt taccgccaac atgcccaaac ctttctcgct catgctgacg
    12601 atggctcctt tcaagggcgt gccgagtcgg acggcgggca ggccgccgcc tttgtgcata
    12661 atgtcggcaa cgcgcaaaag caggcgtttg ccgaggctgc cggcagggtg gatcaaggcg
    12721 aagtcgtcgg gcgtgaacgc gcgggcgcgc agcaggacga ccgccaacgc gtcgcccaaa
    12781 gccataacgg cggtggtgct ggtggtcggg gcaagcccca acgggcaggc ttcttgcgaa
    12841 accgatgcgg tgatgtggat atcggcatgg cgcgccatgg ttgaatcggg gcgggcggtg
    12901 atgcagacga gggtaatgtc tttgcgtttg agcgcgggga tgatggcggt gatttcgtcg
    12961 ctttcgccgg aattggaaat ggcggcgacg acgtcgttgt ccacaatcat gcccaaatcg
    13021 ccgtgtgccg cttccgcagg gtggacgaaa aacgcgggcg tgccggtcga ggccatggtt
    13081 gccgccattt tgcgcccgat atgtccggac ttgcccatac ccgtgatgac gaccctgccc
    13141 ttgcagtgca acaacgcgtc tgccgcaagg acgaagtttt cgtccaattc cgccgcaatt
    13201 tcgcgcaagc cttccgcttc ggtgtgcaac acttcgcgcg cccagtcgag atatttttcg
    13261 ttttctgcca ttgccatccg cctttgctgt aacaaaacta cccaaaattt ccgaatcggg
    13321 gtagcataag ccttcaaacc ccgcccatcc atccaaagga atcaaaaatg actattttat
    13381 cggacgttaa agcattagga caacaaatct ggctggacaa cctttcccgc tcgctcgtgc
    13441 aaagcggcga attggcgcaa atgctcaaac aaggcgtgtg cggcgtaact tccaatcccg
    13501 ccattttcca aaaagccttc gccggcgacg cgctttatgc cgacgaggtc gccgccctca
    13561 agcggcaaaa cctcagcccc aaacaacgct acgaaaccat ggcggttgcc gacgtacggg
    13621 cagcctgcga cgtttgcctt gccgaacacg aatccaccgg cggcaaaacc ggtttcgtca
    13681 gtctcgaagt ttctcccgaa cttgccaaag acgctcaagg cacggtagaa gaagcccgcc
    13741 gcctccatgc cgccatcgcg cgtaaaaacg ccatgattaa agtgcctgcc accgacgcag
    13801 gcatcgatgc gctcgaaacc ctcgtttcag acggcatcag cgtgaacctg accctgctgt
    13861 tctcacgcgc ccaaaccctc aaagcctacg ccgcctacgc gcgcggcatt gccaaacgct
    13921 tggcagccgg acaaagcgtt gcccatatcc aagttgtcgc cagcttcttc atctcgcgcg
    13981 tggacagtgc gctggatgca acgctgcccg accggctcaa aggcaaaacc gccatcgccc
    14041 ttgccaaagc cgcctatcaa gattgggaac aatatttcac cgcccccgaa tttgccgcac
    14101 tggaagccca aggcgcaaac cgcgtgcagc ttttatgggc atctaccggc gtgaaaaacc
    14161 cagcctatcc cgacacgctc tacgttgaca gcctgatcgg cgtgcacacc gtcaacaccg
    14221 tccccgatgc cacgctcaaa gcctttatcg accacggcac ggcgaaagcg acgctgaccg
    14281 aaagcgcgga cgaagcacgg gcgcggctcg ccgaaattgc cgcgctcggc atcgatgtcg
    14341 aaaccttggc ggcgcgtttg caggaagacg gtttgaaaca gtttgaagaa gcctttgaaa
    14401 aactgctcgc gcctttggtt taggcatttc cccgatgccg tctgaaaggc tttcagacgg
    14461 catcgccctt ttgaccggac acagtgcggc atatcaagga tccgccctaa aaatattaat
    14521 gggcaagaag ttaatgcacg acattacccg ccaacacaaa aggaaataga atcatggcaa
    14581 aagcactcga aatcatttca cccgatgaaa tttattcaga tctgattttt aaggatccgg
    14641 tacctcccca tactatatat acagaattga tgaaactggt tggtagaaat gcaggcaatg
    14701 agagaattgc agatagagcc ttcgattttt tctcgcctgc tcttatggat gattcagcta
    14761 ccgaagagca atataatgcc ctatatgatt taaccttgct tgaagagccc gggatggaac
    14821 tggataaaga tgagataacg gctttgatta atagtcttaa ataaaggggc tgtaccggat
    14881 atgtacacag gacgcttcgc ccccagcccg accgggttgc tccacatcgg ctctcttctg
    14941 accgccgtcg cttcctatgc cgatgcacgt tcaaacggcg gcaaatggct ggtccgtatg
    15001 gaagacctcg atccgccgcg cgaaatgccg ggggcggcaa gccatatcct gcacacgctc
    15061 gaggcattcg gattcgagtg ggacggcgaa gtcacctatc agagccgccg ttacgccctg
    15121 tatgaagaaa ccctataccg tctgaaaacc gccggactgg tctatccctg ccattgcaac
    15181 cgcaaagact ggcaggccgg ggcaaggcgg ggcacagacg ggttcgtcta taacggacgc
    15241 tgccgccacc ccggccaacg ccctgcactg caaggcaaac agccgtcgtg gcgcatccgc
    15301 gtccccgacc gcgacatcgg cttttcagac ggcatcgtcg gcagttacac ccaaaacctc
    15361 gcccgcgaca tcggcgattt cgtcctgttc cgtgcagacg gttactgggc ataccagctc
    15421 gccgtcgttg ccgacgatgc cgaacagggc gttacccaca tcgtgcgcgg acaagacctg
    15481 ctcgtttcca caccgcgcca aatctatttg cagcagtgtt tgggcgttcc gacaccgcaa
    15541 tatgcccacc tgccgctgct gaccaacgcg caagggcaga aatggtcgaa gcagacgctc
    15601 gcccccgcat tggattcaaa ccgccgcgaa caacttctcc gccaagtgtt ccgctacctc
    15661 aagctgcccg aagcaccgga aaccgaccgc cctgccgaat tgctcgactg ggcggtggca
    15721 cactgggata tggacaaagt gccgaaacac gccattaccg ccccctaaac accacccgac
    15781 aaacgcacaa atgccgtctg aaaccttcag acggcatttc acacatacag ccccgcccta
    15841 ttcgcgcgta tttgcccgtt cgacttcttt ccacgcttcg aaaatcaggc cgccgtcgtt
    15901 gtctttcaag agcgttgcat cggaaagcat acgccgccaa gtccgcgcgc ctttcaaacc
    15961 gtgcatcagc ccgaggctgt ggcggacgat gtggcgcagg attgtgccgc gtccggcttg
    16021 gatttggact tggctgtatg tgtagaggcg ctgcaccaaa tcggcgtatt cgatcggggc
    16081 gcggttgtca ccgtaaaaca gcctgtccca ttcgcgcatc accatcgggt tgtggtatgc
    16141 ctcgcgcccg accatcacgc cgtcaacgtg ttgcaagtgt ccggcgattg cttcgttggt
    16201 ggtgatgccg ccgttgatga tgatttccag ctcgggaaac tcctgcttga ggcggtaaac
    16261 gtaatcgtat ttcaagggcg gaacatcgcg gttttctttg ggcgaaagac cgtccagcca
    16321 agcgttgcgg gcgtggacga tgaaggtttt gcaggcggtt ttgtcgcgca gcgtgccgac
    16381 gaaatcggca acggtttggt attcggtctg cctgtccaca ccgatgcggt gtttgacggt
    16441 aacggggatg ccgaccgcgt cgtgcatggc gttgaggcag tcggcaacca gcccgacttc
    16501 gttcatcaga cacgcgccga acgcgccttt ctgcacgcgc ggactgggac agccgcagtt
    16561 gaggttgacc tcgttgtagc cgtattcttc ggcggctttg gcggctttcg ccaaatcgga
    16621 cggatcgctg ccgcccagtt gcagggcgac gggctgctcg ccttcgttaa acatcaaaaa
    16681 acggtctttg tcgccgtaaa caatcgcgcc ggcgttgacc atttcgctgt aaagccaagc
    16741 atttcgggta atctggcggg caaggtaacg gtagtgcctg tccgtccagt cgagcatcgg
    16801 ggcgacgcat aaacgccttt tccctttatg ttcaataagt tccttgttta tcataaggtt
    16861 ttcacattct tttaactcaa acattcttta ttatttgctt ggatttttgc ccatttcact
    16921 atatttgacg taccatactt gcaccataat ttttaattct tggaatggtg caaatggcaa
    16981 caataaccaa gcggcgcaat ccttccggcg aaacagtata tcgggttcag gtacgggtcg
    17041 gcaagaaagg ctatcctgct ttcaatgaga gcaggacgtt cagtaaaaag gctttggcgg
    17101 tcgaatgggg gaagaaacgg gaggcggaaa tcgaagccgg cccagaactg cttttcaagc
    17161 gcggcaaggt caagatgatg acgctgtccg aagctatgcg gaagtatctt aacgaaacgc
    17221 ttggagcggg tcggtcaaag aaaatgggct tgcgttttct gatggagttc ccgattggcg
    17281 gcatcggcat cgataagctg aaacggtctg atttcgcgga acacgttatg cagaggcggc
    17341 gcggaatccc cgaactggac attgcgccaa tcgcggcttc tacggcattg caggagctgc
    17401 aatatatccg ttccgtgcta aaacacgcct tttatgtgtg ggggcttgaa ataggctggc
    17461 aggaattgga tttcgcggca aacgggctga aacgctcgaa tatggttgcg aaatctgcaa
    17521 tcagggacag attgccgacc acggaagaac tgcaaaccct gacaacttat ttcctgcggc
    17581 aatggcaaag caggaaatct tccataccga tgcacctgat tatgtggctg gcgatttata
    17641 cgtcaaggcg gcaggatgag atttgccgcc tgctgtttga cgactggcac aaaaatgatt
    17701 gtacccggcc ggttcgtgat ttaaaaaatc cgaacggcag cacagggaat aataaggagt
    17761 ttgatattct gcctatggct ttgccggtca ttgacgagct gccggaggaa tcggtcagga
    17821 agcgtatgct tgccaacaaa ggcatcgccg acagccttgt accgtgcaac ggaaaatcgg
    17881 tttccgccgc ttggacgagg gcgtgcaagg ttcttagaat taaagacttg cgcttccacg
    17941 atttacggca cgaggctgct acccgtatgg ctgaagacgg cttcacgatt ccgcaaatgc
    18001 aacgggtaac gctgcatgac ggttggaaca gcctgcagag gtatgtgagc gtacgcaaac
    18061 gctcgacgcg gctggatttt aaagaggcaa tgatgcaggc gcaaagcgat ataaaatccg
    18121 ggaagtgatg ttaaattaaa ggggatgtgc cgcatcccct gattcctccg tcttgcatca
    18181 agaccggaat ttccgatatt ccgattttgc cttttcttcc ttgtctgcga ttgcctttgc
    18241 aagctctcgg acgctgacga aatattccga ttttcggctg tcggacaatc ggaaaatcgg
    18301 aaacggaagc tcgcaccgta cccccgatgc tttaaacgcg ttaaagccga tatgggcaaa
    18361 aaagtctttg tgtacttccc tcggcgggat atacgggcgg ccgccgtatg cgattaaaag
    18421 cccttgttct ttactgatgt tcatatatac ctctttctgt taattctgct ttgtccgggt
    18481 ttccgctttt ttacggcttg gcggatgttc agcctgcgtc ggtgcataag tcctcctgtt
    18541 cgggcggttg ttcagccggc cgcccctccg aaaagtggca ggctgcacgg gaagtagtgt
    18601 ttttttccgt atttctctgc ctgtatcagg ttgcacaggc ggtcgcaaaa gccgtcaaat
    18661 tcggcatgcg gccagccttc tatctcgttg accattttgc tgaacctcaa ttcccgggca
    18721 atccgctttg ccgctgcgtc acggcattcg tgttccaaga ggacggggtc ttccttttcc
    18781 ttcggcggtg caggttcttg cacggtttgc cacaagtccg gttcgatttg ccaagtgtcg
    18841 gcggcaagca gggtgtctgt tttgcagtcg tacagttcgc ggatgcagcc gccgtcggtt
    18901 ttgtctttgt cgataaccag taaaagcact tcgatcggcg tgtcttcaaa cgcgttttgt
    18961 atgcggttga gttctgccag ccggttgccg atgatttcgc ggaatcgctg ctcgcttttg
    19021 cggtaggcga tgccggggaa caggatgaag aatccgaagc ggcgggcgtt ttccaatcct
    19081 tttaaaacga agatttcatc ggctacgccc gatttcttcc acgggtattc ctgtgcgata
    19141 cggctttttt cgtcttcgct caaatccttt agcttgatgg aaaacggcgg attcatgacg
    19201 gtgcagtctt gcggttcgcc gtcggtataa aggaaaaggc ttgtgttgta tactttggcg
    19261 gcagggtagt tttgcaacaa ggctttacac gcttccgcct gtatctctac ggcgcggaaa
    19321 tcggacggtt cgatgtactg ctccagctgc ccgcttcctg ccgcgccgtc aaatacgccc
    19381 gggtgttcgc cgcagtatcg gcggactttg gcggcaacca gccgcctcaa gctgccgccc
    19441 gtgatgtatt cggcgtagcc gccggctttt ttacggttgt tgtgtccttg aaacgtcatg
    19501 ggttttcgtc cgttctctgg cttgtccggg tgtggcggag cgtttgcgga tgcggctgac
    19561 gcgctgcggt ttgccgtctt cgccgcgctg aaaaacgctt gttccgccgc gccggcagcc
    19621 gtgccgctta tgcctgatgc cgtgtttgaa gccttttgtg ccggaatcgt gatacacgca
    19681 ggtccggatg tgcgtcctca tgccgccgct tccctgtgca tgaggctgat caggtttgcc
    19741 gtccgtttga aatgttcggt ccagttgaag cagctgaagc cggcgccgtt gtcgcaatgc
    19801 cggatgacgt cttccgagtc ttttacggcc cggaccaacg cgccgccttt gtccgcaaca
    19861 agcattttca tcatcggccg gcgcacgtcg cttatccgtc gggggcgcac gggattcagc
    19921 ggcctgccgt acatcgccga aagctccttg tccgcctgta tgcccacatc gtaaagcagg
    19981 ttcgcggcgt gtaggatccg gcctgtaaat tccgcgtacg gcattttaag gcggcgggct
    20041 tccgcgcgcg aatccgcgcc gttgacgact ttgccgaccg ccgaaatcaa accgccgtca
    20101 agttctaaaa acgccatggc tttcttggca acccgcacgg ccaatgagta tgccgatact
    20161 tcggctatca gccgtgcagg cgcgtctttc agaaattctt cgtacgcggc gaagagattt
    20221 gacagcggac gcagcacgag ttggcgctga ttcgggctaa ggtcgtcgaa atccttagtc
    20281 cattgcgcga ccgcctgcga tgcttcgcgg caggcaaaca gtacgctttc c
//
LOCUS       DAWTCI010000030        20227 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWTCI010000030 DAWTCI010000000
VERSION     DAWTCI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20227
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.030"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            8..654
                     /locus_tag="WM481_001092"
                     /pseudo
     CDS             8..654
                     /locus_tag="WM481_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(726..2471)
                     /gene="recD"
                     /locus_tag="WM481_001093"
     CDS             complement(726..2471)
                     /gene="recD"
                     /locus_tag="WM481_001093"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFA9080418.1"
                     /translation="MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQN
                     GHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
                     LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLSPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAVNTVKVR
                     QTALGSMLERVFSQE"
     gene            complement(2538..3233)
                     /gene="lolD"
                     /locus_tag="WM481_001094"
     CDS             complement(2538..3233)
                     /gene="lolD"
                     /locus_tag="WM481_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFA9080419.1"
                     /translation="MSDLILKCEGVGKRYRDGGLDVPVLYGLDLEIRTGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRALNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSRKEAVETAMAMLDKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPRQGANA"
     gene            complement(3226..4473)
                     /locus_tag="WM481_001095"
     CDS             complement(3226..4473)
                     /locus_tag="WM481_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFA9080420.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFAENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGGKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTYSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLWGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            4699..4977
                     /locus_tag="WM481_001096"
     CDS             4699..4977
                     /locus_tag="WM481_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080421.1"
                     /translation="MNIRKISALCAVAVFTVSTAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPVSLNGGSGSITVSGSSAAQCVFTVGSGGTLQIQGLNCK"
     gene            complement(5033..5650)
                     /gene="recR"
                     /locus_tag="WM481_001097"
     CDS             complement(5033..5650)
                     /gene="recR"
                     /locus_tag="WM481_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HFA9080422.1"
                     /translation="MNSKKQDAFQRLIGALKVLPNVGPKSAQRMAYHLLQQKRKEAEE
                     LVDALQTALRQVRHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNIEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLDGGEIEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(5717..7255)
                     /locus_tag="WM481_001098"
     CDS             complement(5717..7255)
                     /locus_tag="WM481_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFA9080423.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISEHSINNAMQNEQADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIV
                     DDPNFHDANGKFSHALLSQYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKNLLFEEAKDAVRQAYIRTEAAKLAENKAK
                     EVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAVTPPEDIAAQLPPAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(7304..7678)
                     /locus_tag="WM481_001099"
     CDS             complement(7304..7678)
                     /locus_tag="WM481_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFA9080424.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFGFRDFKKQMPSEAEICS
                     WLEQVPLATLFNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGSRV
                     YVGFSEETYDGIFNRQAPCRQG"
     gene            7828..9456
                     /locus_tag="WM481_001100"
     CDS             7828..9456
                     /locus_tag="WM481_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFA9080425.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFRNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGREGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALDKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            9535..10767
                     /locus_tag="WM481_001101"
     CDS             9535..10767
                     /locus_tag="WM481_001101"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFA9080426.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPKTHEEYALARTERKTAKGYAGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRAARFAARYGFEIAEETIKLMRQM
                     VENGEADALVAERVWQELAKGLMEKNPRKMIEMLRECGALQVLLPEVDALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPPDILPRHHGHDINGVE
                     PVREVNQRLRAPRQCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRAQGKAHFIAEQI
                     DRARLAQIAPLQKAFRGA"
     gene            11186..11494
                     /locus_tag="WM481_001102"
     CDS             11186..11494
                     /locus_tag="WM481_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080427.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGETRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            11576..12607
                     /gene="ruvB"
                     /locus_tag="WM481_001103"
     CDS             11576..12607
                     /gene="ruvB"
                     /locus_tag="WM481_001103"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFA9080428.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMGEEGA
                     MEVAKRSRGTPRIANRLLRRVRDFADVKNNGVIDAAVADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(12852..13535)
                     /gene="rpe"
                     /locus_tag="WM481_001104"
     CDS             complement(12852..13535)
                     /gene="rpe"
                     /locus_tag="WM481_001104"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFA9080429.1"
                     /translation="MTAYRIAPSILSADFARLGEEVANVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYVLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRQV
                     RAMLDRYEGKSGRRIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIAAM
                     RAELEKAAG"
     gene            13681..14115
                     /locus_tag="WM481_001105"
     CDS             13681..14115
                     /locus_tag="WM481_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFA9080430.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYHSSMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(14128..14991)
                     /locus_tag="WM481_001106"
     CDS             complement(14128..14991)
                     /locus_tag="WM481_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9080431.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHVFPLSRLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVMVYATSNRRHLMPEYLDENAGTTGVRGEIHQKEAVEEKVSLSDRF
                     GLWLSFYPFDQNDYLAAVRSWLEDFDVPYDEAARTAALQWAQMRGNRSGRSAWQFACD
                     WAGRLPEQRAP"
     gene            complement(14999..15616)
                     /locus_tag="WM481_001107"
     CDS             complement(14999..15616)
                     /locus_tag="WM481_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFA9080432.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAAENLQHGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFALPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKAGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(15779..16393)
                     /locus_tag="WM481_001108"
     CDS             complement(15779..16393)
                     /locus_tag="WM481_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFA9080433.1"
                     /translation="MKTSNFPKSPPALKTFALILAGGLADRMGGEDKGLALLEGRPLI
                     DRVIGKIRLQVSHIVISANRNLEEYARRSPHVFPDARQWQHFGPLSALCTAANDLQLA
                     AADWLLIVPCDMPYLPDDLVARFESVSKRTPLCNAFYVETPVTMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            16600..18369
                     /locus_tag="WM481_001109"
     CDS             16600..18369
                     /locus_tag="WM481_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HFA9080434.1"
                     /translation="MILPTRFSDGIPLSLRLKLLTGLWVGLAALSVVLTLLLSFRLEN
                     AASVIEEAGNLKMQAYRLAYMAGEGSPRAQIDNQIAEFEKSLKRISQSDAIHPLIPSD
                     NPLAYDLIQSMLIIDWQANILPPLQAYRRPTQIELYRFAGNIELFLQALENAGEKNTW
                     WLRRFQWVIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDGTPE
                     FKQVGRCFNQMALRLKTLYDDLEGQVAEQTHNLEKQNRNLTLLYRTTRDLHQSYTPRQ
                     AAEEFLNHILPAVGAQSGNICLENGSDTDISVHTAEHGKKPPLEKYHDETFPIEYQNE
                     KLGMLSLGFSDGTSLTGDDRTLLQTLIRQLGVSLAGAKQEEEKRLLAVLQERSLIAQG
                     LHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFRT
                     KISNKEFPEAVADLFARFTQQTGITVETVWENGSFLPTQDEQLQMIFILQESLSNIRK
                     HARATHVKFTLSEYGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAVL
                     EIRSQAQQGTTVSLTGAPKESLP"
     gene            18366..19022
                     /locus_tag="WM481_001110"
     CDS             18366..19022
                     /locus_tag="WM481_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9080435.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQRTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
     gene            complement(19178..20086)
                     /locus_tag="WM481_001111"
     CDS             complement(19178..20086)
                     /locus_tag="WM481_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFA9080436.1"
                     /translation="MRFPLPITNAVLKITNGAMEFKSEDIGTIKLTKATFHLKKDTSL
                     TTPEGTTLLSGGTLTLSNTGISLSGTTSVFEKGTFTNGGIITLANQSYADKLTIEGNY
                     VGKDGVLKVNTEWNSPGDDQGGNSQSDLLEITGDASGKTTVISVGKDGKENIIDGSIG
                     ELSDRYKRSAAVVKVLGQDKGAETGKLNIEDAKHTYTMRDTFSGTAKTTGAGELQLVS
                     HKDEAGATEYFWTLTTPNQDKTIITPSAPAYALVPRQNLESGYAMLDTLHQRRGENQT
                     LSRDRQGNYRQDAEATDIKATKAPWV"
BASE COUNT         4668 a         5846 c         5061 g         4652 t
ORIGIN      
        1 tatataaggg gttgccgttc cgcagttggg cggcagcata cacgttcctt tcggcgtatt
       61 ctcgccgttg cgcggcgaat atctcgacct gcttgcccat gcgccgtcaa cgggttttca
      121 gacggcattc gatatcggga cgggctcggg cgtacttgcc gccattttgg cgaaacaggg
      181 cattccttcc gtcatcggca cggataccaa tccgagggcg gttgcctgcg cccgtgccaa
      241 tattgcccgt ttgggttttg aaaaacaggt tgaaatccgc gaaaccgatt tatttcccga
      301 agggtttgcc gacctgattg tctgcaatcc gccctggctt cccgccaagc cgacttccgc
      361 cgtcgaatcc gcgttatacg accccgaatc tgcgatgctg gctgcgtttt tgcgtgatgc
      421 gccaaacatc tgaatcccga cggagaaatc cgtcttatca tttccgacct cgccgtgcat
      481 ttggggctgc ggtctgccga ctttttggaa aaggcattta tccgggcggg tttgcgtgtt
      541 gaggatgttt tgaaaaccaa accggttcac aaaaaagctg ccgattcgaa cgatccgctg
      601 gcttttgccc gaaacaggga aactactttc ctgtgccgtt tgaaaaaggc ataaggggcg
      661 gtggcgtgct tccgacggta ggcatcggaa cgcccgcgcc gccggcacgg catcattcgg
      721 gcggattatt cttgtgaaaa tacccgctca agcatactgc ccaacgccgt ctgacggact
      781 ttgacggtgt ttaccgcctg acaaaatgtc tttttgccgc cgaaaaatac gaatttttcc
      841 ctggcgcggg taatggcggt atataacagc tccttactca atccggacaa tgcatcgtcc
      901 ccttcgtccg aaggtgcgtc ggaaggcggc agcagccaga cttcccgata ttccgaacct
      961 tggcttttgt ggacggtcat ggcgaatgcg ggttcaaatt cgggcaggca gcttaccgct
     1021 acctttttaa atccgtccgc atcggcaaaa taggcggcaa ggctgccctg ccgtccgaca
     1081 tcttccataa tcagtccgat gtcgccgttg aacagctcaa gcgcgtagtc gttctgtctg
     1141 atcataatcg gctctccggc gaaataggcc aaatgttccg gtatgttcat tttgcggcgt
     1201 acatagctgc aataggcttc gttgaagtct tccgcatcct gccgccaagc tgccagaacc
     1261 acgatatccg agatgcctgc gtatgcggct tcgatattgc cgtcttttac cgcctgccaa
     1321 taggctttgt gcgcccggta caacctttcg acccgagcat tcggactgca ttccgaatgt
     1381 tccagttcgt ccggaaaccg gtcaaacaat gcccacgccc cttcatcgcc cgatacggcg
     1441 gcacgggcaa ggcagccgat gccgctgttg tcgccgaagc ggtggctgaa cgacagatgg
     1501 gcggtgtttt gcgccaacac gggcggattt gcgctgacgc tgaaaccgtg ttccggaagg
     1561 aaaccggcca gcctttggtg cgtttctccg tccaaaacgg ttttttgcga caaaaccgac
     1621 agcaccgccc cgattccgac ggacgggagc tggttttcat cgcccagcag aatcacgcgc
     1681 gcgccggttt tgaccgcttt caaaagttgc agcatcaatg cggtatccaa catggaggct
     1741 tcatcgacaa tcagcacgtc gaacggcagc ggacggatat ggtcgaacgc cgcctgcatt
     1801 ttgggcgggc tcagcttaag cagtcggtgg acggtttgcc cttccagttt gagcaaatgg
     1861 cggcggacgg cctccggcgc gtcaaaaccg ttgattgcac ggtgcagcgc gcgcgccata
     1921 tgtgccgccg ctttgcccgt cggtgcggca agcgcgatat ggggaagatt ttcgttttca
     1981 ccgcaaatca gtgccagcag tttggcaacc gttgtcgttt tgcccgttcc cggccctccg
     2041 gtaatcacca taaaaaactg caacagtgcc aaggcggccg catcgcgctg tccttcgctt
     2101 cccgcgcctt gaaaccattt tgcgaggttt tgccgcgcgc ctgccgcgtc gggggcggac
     2161 gtgccggctg ccgccaagcg ttttatttcg gcagccaaat cgtattccaa ctgccacatc
     2221 ctgcccaaaa acagccttct gccttccaaa atcaaaggcg cggcggatgt tccgaccacg
     2281 ggtgcgagtg ccgacagcgc gtcagcctcg tccccgctca aacggataaa cgaatgaccg
     2341 ttttgcaatg cctgaaacag gcgttcggta cagtttgcaa gcacttcgtt gcccgaaccc
     2401 gcatagcgtt ccaaaaaacg gattgccgcc cttgccgccg cttgggcaaa ttcatctgtc
     2461 tgcagttcca tcgtattcct tgtcgaaatg ccgtctgaag gcacggggct tcagacggtg
     2521 cgtgttgttt cggctgttta ggcgtttgcg ccctgtctgg ggtgcaggct gccgtctttc
     2581 atgaccatca cgcgctcgaa gcggccggcg agttcgtcgt cgtgcgttac gaccaccagc
     2641 cccgttccca attccgtttt cagttccagc atcatatcca aaacattcct tgcgtttgcc
     2701 cggtcgaggt taccggtcgg ttcgtcggca agcaggcatt tgggttgcgt aaccaaggcg
     2761 cgcgcaatgg cggcacgctg gcgttcgccg ccggacaatt cgcccgcacg gtgcgtcgaa
     2821 cggtgtttca gtccgacctt gtcgagcatc gccattgccg tttctaccgc ctctttacgg
     2881 ctttttttgc cgatcagaag cggcatcatc acgttttcca gtgccgaaaa ttcaggcaga
     2941 agatgatgga actggtacac gaaaccgaga tggcggttgc gcaaatcgcc caaacgccgc
     3001 tggtttaagg cacgcaaatc ctcgcccatc agcagcaccc tgccttcaga cggcatatcc
     3061 agcccgccca aaatatgcag cagcgtcgat ttgccgctgc ccgaagagcc gatgatgccg
     3121 gtgctttccc ccgtgcggat ttccaaatcc aagccgtaca gcaccggaac gtccaaaccg
     3181 ccgtcacggt agcgtttgcc cacgccttcg catttcaaaa tcaaatcact cataacgcaa
     3241 agcctccgcc ggttgggttt ttgacgcgcg ccagctcggg tacagtgtcg ccacgaaaga
     3301 cagccccaag gaaatgcagg caatcagggc aacgtcgccc atatcgacat cactgggcag
     3361 gtagtcgata aaataaacct gcgaattgat gaggtggaca ccgagcaggt tttcaaaaaa
     3421 cgccacgacc ctgccgacgt tccaacccca aagcacgccg cagaccacac ccgccagcgt
     3481 gccgaaaaag cctgaaaacg cgccctgcac cataaaaatc ttcatcacgc cggcagggga
     3541 aagacccaaa gtccgcaaaa tcgcaatgtc cgcctgcttt tccgtaaccg ccatcaccaa
     3601 ggaagagaca aggttgaacg ccgccacggc gataatcagc gtcaggataa tgaacatcat
     3661 ccgtttttcc agttcgaccg cttcaaaata gctgcggttg ctgtacgtcc aatcgcgcac
     3721 ccaaaccgtg tccctttgcg cctccggaat cagcgttgcc gtcaaggcgg gggcgttttg
     3781 cggatcggca agcttcagcc gcagccccgc aacttcctta tccaagcggt acagtacgcg
     3841 cgcgtcttgg atatgcgtca tcgccaatga gttgtccact tcgtaaacac ccgtcttgac
     3901 caggccgacc acggtaaact gcttcaggcg cggcacgact ccggcagggg taacgttgcc
     3961 ctccggcgta attaccgtaa ccttgccgcc gacttccgcc cccaaagcct ccgccaaacc
     4021 gacaccgagg ataatgtcaa actcgcccgg aatcaggtct tcaaacttgc ctgccggcat
     4081 tttgtcgccg tattccacca ctttgcgctc ttcagacggc aaaatgccgc gtatctgaac
     4141 gcccctgatt tcgcccgcgt ttgccagcaa tgcctgattg gaaacatagg gtgcggcagc
     4201 caaaatgcct ttgcggtttt cggcaaaccg aagcaggttg cgccaatccg tatccgtatt
     4261 gtcgatatag ccgatttcgg cgtgcggcgc gacattcagg agctgcccgc gtatttcttt
     4321 ctgaaagccg ttcataaccg acaagacgac aatcagcgcg gttacgccca aggcgattcc
     4381 ggcaatcgaa accatcgtga taaacgacat aaagccgttg cgctttttcg ccctgagata
     4441 cctcaagcct atccaagcct ctagagaaaa cataacgcta ccttaaaaat gtctgcaaac
     4501 gtgccgcccc ggacggcggt ttgggggagg cggcaaaagt ttattgtacc gtaaaacccc
     4561 ggcagcgtcc gaacggcagg ctgtgtaaat aaagacgcgg tatgtggcaa attcggaaaa
     4621 acaggacgga aaacttgcac aacgcgccga actaccctat ccttctcttg aaacaaaacc
     4681 ttttctttaa ggaaaacaat gaatatcagg aaaatctccg ctttgtgtgc tgttgccgtt
     4741 tttactgttt cgacagccta tgccaaagaa atcaaaatcg atgccaacaa cacgccttat
     4801 tccgaagccg acgcgcaaaa gctggcggca acggcagtcg gtatgggcgt taaggaacct
     4861 gtcagcctga acggcggcag cggcagcatt accgtttccg gcagcagcgc ggcgcagtgc
     4921 gtctttacgg tcggcagcgg gggcactttg cagattcaag ggctcaactg caagtaaacc
     4981 gcccgaaaaa atgccgtctg aaggcttcag acggcatttt gcattggcgg cgttacgccc
     5041 cgccttcttt aatcagacgc cgttcgtaca ccgcctgcgc cagcgttccc gcatcgacat
     5101 attccaattc gccgcccaat gggatgccct gcgacagcct gctgaccttg taaggcaggt
     5161 ttttgaaaaa ctcggacagg acatacgctg tggcattgcc ttctgcggta aaggcggtcg
     5221 cgataatgat ttcttcgatt tccccgccgt ccagccgttg cgccagcctg tccaatgcga
     5281 tggcggatac gtccattccc aatgccgtat tgatttgccc catcaggacg aaatacagcc
     5341 cgtcgtggca gtttgccgct tctatattcg acacgtcggc aggcatatgc accaccatca
     5401 gccgccgccc gtcgcgtgtt tcatcggcac aaatatcgca caatccgcct tcgcaaaatg
     5461 tattgcacct tgcgcaatgg cgaacctgcc tcaatgccgt ctgtaaggca tccaccagct
     5521 cttcagcctc tttgcgcttt tgctgcaaca gatggtacgc catccgctgt gccgatttcg
     5581 gcccgacgtt gggcaatact ttcagcgcac cgatcaggcg ttggaatgca tcctgttttt
     5641 tggaattcat catactccgc catatgggaa aacggtcgga atattccgac cgttatgttg
     5701 tcaacaaaag tgtcaattac tgaccatcgc cgttgtcaac cgattgtgct cctttagtct
     5761 gtttgatttt tccgttgaaa tagcggatca gcaggtcgaa agtattggca gactgctgtt
     5821 gcgccaaagc ctgtttcgca ggaggaagct gtgcggcaat atcctccgga ggcgtgactg
     5881 cctgcacctc gacaatcacg ggtgccggca gaccggtcag tctgacatag gcgggtttgc
     5941 cgtttgccgg ttttgctttc agcagttccg cataagcctc gggcggcatg gactgccttg
     6001 cctgctgcgc gcccaaaacg gacacttccg accatttcac gtcaactgcc ttgccgccgt
     6061 tcagttgggt aagcacttct tttgccttgt tttcggcaag tttggcggct tcggtacgga
     6121 tataggcctg acgcaccgca tctttggctt cttcaaacag taggtttttt tcttcgcgga
     6181 cttctttggc gcggacgacc cacgcggttt cgctgttgat ggtcagcact tcggaattgt
     6241 gttttttctt caatacgtcg tcgctgaata cggcattgat taggttttcg ggcatgccgg
     6301 acatttgtgc gtcctgcctg ctcagccaag tttcttgggt ttccactttc aaaccgctgt
     6361 ttttggcggc ttcggcaagc gaggagggat gattgaacgc atcgtcgccc agcttttctt
     6421 ttgccttgtt gaagtcggca accgcctttt tcattttcaa ttcgttttcg acggcggctt
     6481 tttcctgctc gaaagaaggt ttggcttcat gtgccggcaa acgcgccacg cgctcttcaa
     6541 acgcattttt cacttccgtt tcactgacgg tctgcttgtc tgcaaaatcc ttcagattca
     6601 aggcgacata ttccaatttg accgcctgcg gcagcagata gtcttttttg ttcgcattat
     6661 aaaatttctg caaatcggct tcagacgctt tgacttgggc gatgaactcg tcggggttga
     6721 aagtgtgcga acggatggtg cggttgacct gcgtcagcct gatcagctgt tccgcctgcg
     6781 cgtcgccgac caatacgccg ttttggacga ggcttaccaa attctgcaag gcaaactgat
     6841 cgcggatttc ttcgacaaac tggtcttcag acatatggcg ttgcgacagg tattgactca
     6901 aaagcgcgtg actgaatttg ccgtttgcgt cgtggaaatt gggatcgtcc acaatcatct
     6961 gcttgatttg ttcggaagaa accgaaatgc ccatcagctt cgcaccctgt ttcaggtagg
     7021 cgcgttgcag cagggattgg aacaccgcgt cgcgccaagg gctgccgccg tccgcctgct
     7081 cgttctgcat ggcgttgttg attgagtgct cgctgatttt ttcgtcgccc acttggacga
     7141 tgtagtcggc gcccggatgg gaaaccgtgc tgacgccgaa gccgacaaaa gttaatgcaa
     7201 tcaggcctaa taagacttgg gcgggtgttc tgtatttttc gatggaatgg aacatatttt
     7261 cagattggga atgggaaatt caagtcgggt attgtaacgg tttttatccc tgtctgcacg
     7321 gggcttgccg gttgaagatg ccgtcgtagg tttcttcgct gaagccgacg taaaccctgc
     7381 tgccgcactc caatacggga cgcttgatca ggctcggcat ttcggacatc agtttgacgg
     7441 cctccgccgt cgaggacagg gctttttgct gtgtttcggc atcgagtttg cgccagcttg
     7501 tcccgcgttt gttgaacagg gttgccaaag gcacttgttc cagccacgag cagatttccg
     7561 cttcagacgg catctgtttt ttgaaatccc gaaacccaaa ctccaagccg tatccggcaa
     7621 gcctgttttt ggcttttttg actgtgtcgc aatttggaat gccgtgaagg attatcattt
     7681 ggaaaccttt tgcctgaaat aataaaaccg atattttact ataagtgtct gaaaatttgc
     7741 ccgtctgttt cagacggcgg ggcggttatg ttacaatccg aaaattcgaa aaatttaatc
     7801 tcttgttcaa taaaggtttt acccataatg atttctacca acggcattac catgcagttc
     7861 ggcgcgaagc cgctgtttga aaacgtatcc gtaaaattcg gcgaaggcaa ccgctacggc
     7921 ttgatcggcg cgaacggttc gggcaaatcc accttcatga aaatcctcgg cggcgatttg
     7981 gaacagaccg ccggcgaagt ggcgattgaa aacggcgtgc gtttgggtaa attgcgccaa
     8041 gaccagtttg cctacgaaga catgcgcgtg ctggacgtgg tgatgatggg gcataccgaa
     8101 atgtgggcgg cgatgaccga gcgcgatgcg atttacgcca atcccgaagc caccgaagac
     8161 gactacatga aagccgccga actggaagcc aagttcgccg aatacgacgg ctacaccgcc
     8221 gaagcgcgcg ccgccgaatt attaagcggc gtgggcattt ccgaagattt gcacaatgcg
     8281 aaaatggcgg aagtcgcccc gggcttcaaa ctgcgcgtat tgctggcgca ggcgctgttc
     8341 tccaagccgg atgtattgct cttggacgaa ccgaccaaca acttggacat caataccatc
     8401 cgctggctgg aaggcgtgtt gaaccaatac gactccacca tgatcatcat ctcgcacgac
     8461 cgccacttct tgaacgaagt ttgcacccac atggcggatt tggactacaa caccatcact
     8521 atttatccgg gcaactacga cgactatatg ctcgcttccg cccaatcgcg tgagcgcgcc
     8581 ctgaaagaca atgccaaagc caaagagaaa ctgcaagagc tgcaagaatt cgttgcccgt
     8641 ttttccgcca ataaatccaa agcccgtcag gcaaccagcc gtctgaaaca ggccgacaaa
     8701 atcaaatcgg aaatggtcga agtcaaacct tctacccgtc aaaacccgta tatccgtttt
     8761 gaagccgatg aaaaagccaa gctgcaccgt caggctgtgg aagttgaaaa actggccaaa
     8821 cgctttgaaa cccagttgtt tagaaacctc aacttcatcc ttgaagcagg ccaacgcctc
     8881 gccatcatcg gcccgaacgg cgcaggtaaa tctaccttgc tgaaactctt ggccggcgcg
     8941 tacaaccccg aatattcaga cggcctgttg ccggacgaag gcagcatcaa atgggcggaa
     9001 aaagccagtg tcggctacta tccgcaagac catgaaaacg actttgacgt cgatatggac
     9061 ttgagcgaat ggatgcgcca atggggtcgg gaaggcgacg acgaacaagt catccgaggc
     9121 actttggggc gtttgctttt cggcagcaac gacgtggtaa aaaaagtgaa agttctctcc
     9181 ggcggcgaaa aaggccgtat gctttacggc aaactgttgc tgttgaaacc caatgtcttg
     9241 gtcatggacg aaccgaccaa ccatatggac atggaaagca tcgaatcgct gaacatggca
     9301 ctggacaaat acaacggcac gctgattttc gtctcccacg accgtcagtt cgtttcctcc
     9361 ctggcaaccc aaatcatcga actggacggc aaaggcggat atgaacacta cttgggcgat
     9421 tacgaaagct atctcgagaa aaaaggcgta gcataagcgc acccctccgg ttgaaacaat
     9481 gccgtctgaa gccgcttcag acggcattgt tgataacttt aaaataggaa gcatatgcag
     9541 acttacctcg tcggcggcgc cgtccgcgat tatcttttgg gcctgcccgt caaagaccgc
     9601 gattgggtgg tcgtcggcgc agacgcgcaa accatgttgg cgcaaggttt ccagccggtc
     9661 ggcaaagatt ttcctgtatt cctccatccc aaaacgcacg aagaatacgc cctagcccgc
     9721 accgaacgca aaaccgccaa gggttacgcc ggtttcagtt tccacgccga caaagacgtt
     9781 acgctggaac aggacttgat gcgccgcgac ctgaccatca acgcgatggc gcaagatgcg
     9841 gacggcaaga ttatcgaccc tttcggcggg caacgggatt tggcggcagg cattttgcgc
     9901 cacgtctcac ccgccttcgc cgaagatccc gtccgcatcc ttcgtgccgc ccgctttgcc
     9961 gcgcgttacg gctttgaaat cgccgaagaa accataaagc tgatgcggca gatggtggaa
    10021 aacggcgaag cggacgcatt ggttgccgaa cgcgtctggc aggagttggc gaaaggtttg
    10081 atggaaaaaa atccgcgcaa aatgattgaa atgttgcgcg aatgcggcgc actccaagtc
    10141 ttgctgcccg aagtcgacgc cctcttcggc gtgccgcaac gcgccgacta tcatcccgaa
    10201 atcgacagcg gcatccatac cctgatgacg ctgcaacgcg ccgccgatat gggtttgagc
    10261 ctgcccgaac gctatgccgc cctgctgcac gacttgggca aagccaaaac accgcccgac
    10321 atcctgccgc gccaccacgg acacgacatc aacggcgtag aacccgtgcg cgaagtcaat
    10381 caacggctgc gcgcgccgag gcagtgtgcc gagcttgccg aattggtttg ccgttggcac
    10441 attattttcc accaagtcgg acagcttaaa agccaaacca ttctgaacgt attgaaaaaa
    10501 accgatgcct tccgccgtcc cgagcgtttt cagacggcat tgaacgtctg cattgccgac
    10561 acgcaaggcc gtctgaaccg agaacacacg ccctaccccc agcgcgcgca ctggctcgcc
    10621 ctgctcgaag ccgccaatca ggcggattcg ggcaaaatcg ccgccgaatg ccgcgcgcag
    10681 ggaaaagcgc actttatcgc cgaacaaatc gaccgggcgc ggctggcgca aatcgccccg
    10741 ctgcaaaaag cgtttcgggg cgcgtaggaa aaagaaaacg ccaaacgcgg catcttttga
    10801 ggttcgcccg cattgccgaa acagcgtcgg agaaagggat ggatgacatc ggtttattcg
    10861 gctgcctttt aatattccct tgatgcaggc ctgattagtc ggacaaaaat accttttgtt
    10921 caacggaacc gatttctatc cggacggaaa cagccggctt tccccttgca aacggatgga
    10981 atcaagcggg tattcaaaca cagccgttta tttttattcc gccgccaagt tatgggatag
    11041 ggtttgcgtg tttcgggcag gcaaaccgcg caaccttaga cgcagtccga agttttgaag
    11101 gcggcatccg ggccgggttt taaaacagcc gccgccatat ccgctataat ccatccttca
    11161 gccattccgc ccccgacata aaatcatgac cctgaaaacc gatttattgc ctaaaatcaa
    11221 caacgaagat tatcaacgcc tcatcctcaa acatagcgcg gaattcagcg gcggagaaac
    11281 ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg cgcaggcatt
    11341 ggcgcaggcg gtaatgcagc aagttcgctt cgatcccaac gcctaccaca tcgacagcga
    11401 cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc cgcccctgcg
    11461 cgactatctc gtttggcgcg aaacacgcgg ataaaacgct tttgaccgtt atcttttcaa
    11521 tgccgtctga aacgccgccg accgttcgga cggcataccc gacaaaggga acactatgct
    11581 gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc aaaccgcctc
    11641 cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg actacatcgg
    11701 gcagcataaa gccaaagagc agctcgccat cttcatccaa gccgccaaaa aacgcggcga
    11761 agcactcgac cacgtcctgc ttttcggccc gcccggactg ggcaaaacca cccttgccca
    11821 catcatcgcc aaagagttgg gcgtaaattt gcgccaaacc agcggccccg tcctcgaacg
    11881 cgccggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgacgtat tgtttatcga
    11941 cgaaatccac cggctaagcc ccgtcgtcga ggaaatcctc tatcccgcgc tcgaagacta
    12001 ccggctcgac attatgatag gcgaaggacc cgccgcacgt tccgtcaaaa tcgacctgcc
    12061 gcccttcacc ctcgtcggcg cgaccacccg cgccggtatg ctgaccaacc cgttgcgcga
    12121 ccgcttcggc atcgtctccc gcctcgagtt ttacgaaaac cgcgacctta ccaccatcgt
    12181 cagccgttcg gcacaactgt tgcagctcga tatgggcgaa gaaggcgcga tggaagtcgc
    12241 caaacgcagc cgcggcacgc cccgcatcgc caaccgcctg ttgcgccgcg tgcgcgattt
    12301 cgccgacgtg aaaaacaacg gcgtaatcga tgccgccgtc gccgatgccg ctttaagtat
    12361 gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaattcctcg aagccgtttt
    12421 gcacaaattc ggcggcggcc ccgtcggttt ggacaacgtt gccgccgcca tcggcgaatc
    12481 tacggacacc atcgaagacg ttatcgaacc ctacctcatc caacaaggct ttttgcaacg
    12541 caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc tgcccgtcga
    12601 aaaataaacc atgccgtctg aaaccgaacc gggtttcata cggcattgtt atcagacgtt
    12661 tgcaccggca ccggtttcat cacttaacag aaaccttttg tccggcagga cagattctcc
    12721 ccgatgaaaa taactgtttc cctggcaaac ggttgaaacc cgtctggctg ccggttgaaa
    12781 aataacgcaa tgctgtctga aacagagcta attttcagac ggcatttcta tttcaatcat
    12841 tggctcaagg ttcagcctgc cgctttttcc agttccgccc tcatcgcagc gattaccgcc
    12901 ttgtaatccg gtttgccgaa aatcgccgaa ccggcaacaa aggtatccgc accggctcgg
    12961 gcaacggcgg caatattgtc agttttgatg ccgccgtcca cttcaatggc aatacgtctg
    13021 ccgcttttcc cttcatagcg gtcaagcatg gcgcgaacct gtctgatttt ttccaaagtg
    13081 tgcggaatga agctctgacc gccgaacccg gggttgaccg acatcagcaa aaccatatcc
    13141 agcctgtcca acacgttttc caacacatat acgggcgttg ccggattcaa caccagcccc
    13201 gcctgacagc ccatgtcccg aatcaggctc aagctgcggt cgatatggcg gctcgcttcg
    13261 ggatggaaag tgataatcga tgctcctgct ttggcaaacg attgaattaa atcgtcaacg
    13321 ggttcgacca tcagatgcac atcgatcggc acgcttgcat aaggcttcaa cgccgcgcag
    13381 accataggtc cgaaggtcag gttcggcaca taatggttgt ccatcacgtc aaaatggatc
    13441 agatccgcac ccgccgcaat gacatttgcc acctcttctc cgaggcgggc aaagtctgcc
    13501 gacaaaatac tgggtgcgat acggtaggcg gtcatatttt ttccttcaat gcctttatgg
    13561 gcggactcaa cgcgggaaag cctcgaaaca gcattattaa caaaagttaa ctgccataat
    13621 accatctttc acacggcaat caagtatatt gtccgaaata tccggattaa aggaaaagcc
    13681 gtgccgctgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt aagtatggct
    13741 ttgctttcct gccagctttc ccacgccgcc acggcttata tccccccgaa cgattttcaa
    13801 ccgaactgcg acatacgccg gctcgggctg acacagggtc agcacaatga gctgcgtaaa
    13861 atccgcgccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat gcattccgaa
    13921 cacagccgcc gccgctctgt cgtcgaaatc atttcttcgg atgtttttaa tcggaacgag
    13981 gcgcgcgatt atgtcgaaag ccgctaccac tccagcatgg attttgcggt ggacgaattg
    14041 gaaatccaac accgcttctt ccatattctc acaccgcaac agcagcaaat gtggctttct
    14101 tcctgcctca aataatcccc gaaacactca cggcgcacgt tgctccggca gcctgcccgc
    14161 ccagtcgcag gcaaactgcc aagcggaacg tcccgagcgg ttgccccgca tctgcgccca
    14221 ctgcaacgcc gccgtccggg cggcttcgtc atatggcaca tcaaaatctt ccaaccagct
    14281 tcgcactgcc gccagataat cgttttgatc gaacggataa aaactgagcc acaatccgaa
    14341 tcggtcggac aaagatactt tttcttccac ggcctctttt tgatggattt ctccgcgcac
    14401 gcctgtcgta cctgcatttt catccaaata ttcgggcatc agatgccgtc tgttggaagt
    14461 cgcgtacacc atcacgttgg cgcaacgttg ggacagaccg ccatccaatg ccgttttcaa
    14521 tgccttatag gtttcgtcgc cgctttcaaa tgacaaatcg tcgcaaaaca caatgaattt
    14581 ttccgggcgt tccttcaaaa gtgtcaacag atacggcagg ccgatcaaat cgcttttatc
    14641 gacttcgatc aggcgcaatc ccttatccgc atattcgtgc agcagggctt tgaccagcga
    14701 ggatttgcct gttccgcgcg cgccgctcat caatacgtta ttcgcaggtc tgccggcaat
    14761 aaattgttcg gtattacgca ccagcaattc ggtctgcctg ccgactccgg caagccgcga
    14821 caaagggaaa acgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc ccgcactctg
    14881 ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa gcacggcatc
    14941 caaacgccgc aaaacggcat aggctttatc gagaaattcg ttcaattcca tctctacctc
    15001 actttgcata tctttgcgcc atcagccgtt cgacggtgtc gacgattgcc tgcgtattcg
    15061 gatcgatttc gatgttgatc ctgtctccgg cctttctgct gccgaacagc gtccgttcca
    15121 aagtttcggg aatcagatgg acattgaaac ggctgtcttc gactttgcct atggtcaggc
    15181 tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt tcgtgcggga
    15241 gcgcaaacca aaccgtgcgg ttgaacccgt cccgttcgat ttcgacaatg ggcacggttg
    15301 ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc gccgaaacgt gccgcccgtt
    15361 cgatgttgac gcaatcgcct tccttcagca gccccaaatt ggtttttgcc aaagtttctg
    15421 ccattaaatc gaagctgacg cggtttcctt cgatttcagt aatcgtcagg cagcagccgt
    15481 tattggcgac cgatgcgccg tgttgcaggt tttctgccgc ctcttgcgga agctcgacga
    15541 cataagtctg aaacgcctcc gacgggcggt ggattgccgt cagttttccc aatccttgaa
    15601 caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca aattgtagca
    15661 tttccccgcg aaaaatgccg tctgaaactc cttcagacgg cattgtgcct ccggttcggg
    15721 caaaaaccgc ccggtacgcc ttgacctttc ctttccgcgc cggtcggcgg tcttgcactt
    15781 atccctcctg caaatcggtt tgcgtgttca agtcggcaaa atgcccgtca aactcgaatc
    15841 tgaccggccg cgccctttgc tgctgcaacc agcttcttaa tgttttcata cccgaaaaca
    15901 gataaggaat cgcgctttgc agaatttgcg gacggatata cataatgttg tagtgcatcg
    15961 ttaccggcgt ttccacataa aacgcattgc acaacggtgt gcgtttggag acgctttcaa
    16021 agcgcgcgac caaatcgtcc ggcagatacg gcatatcgca aggtacaatc agcagccaat
    16081 cggccgccgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc gggccgaaat
    16141 gctgccactg ccgggcatcc ggaaaaacat gcgggctgcg gcgggcgtat tcttccaaat
    16201 tccggttggc actgatgacg atatggctga cctgcagcct gattttaccg ataacgcggt
    16261 caatcagcgg tctgccttcc aacagtgcca gccctttgtc ttccccgccc atacggtcgg
    16321 ccaggcctcc tgccaaaatc aatgcgaacg tttttaatgc gggcgggctt ttgggaaagt
    16381 ttgaggtttt catagtttca gtctgccgtt tccgatattt tagaagcgcg tttgaaaacg
    16441 gcgtcgtata aattcatgtt atttgaattt tatcataacg tgatttaaca ctgaaagcat
    16501 tgaggcttaa gttttcctta acctgtcctt tctgagcagt tgtttctaat tccaaagaat
    16561 gatattgttt gcaccgttta aaacgacttt cctccgaata tgatactgcc aacccgattt
    16621 tcagacggca tccccctttc cctgcgcctg aaactcctga ccggcttgtg ggtcggtttg
    16681 gcggcactgt ctgtcgtttt aactctgctg ctttctttcc gcctggaaaa tgccgcctcg
    16741 gtcatcgaag aggcgggcaa tctgaagatg caggcatacc ggcttgccta tatggcgggc
    16801 gaaggctcgc cccgcgcaca aatcgacaat cagattgccg aatttgaaaa aagtttaaaa
    16861 cgcatttccc aaagcgatgc catccatccg ctgattccct ccgacaatcc ccttgcctac
    16921 gatttgatac agtccatgct gattatcgac tggcaggcca acatcctccc cccgttgcag
    16981 gcataccgcc gccccacaca aatcgagctt taccgctttg ccggcaatat cgaactgttt
    17041 ttgcaggcgt tggaaaatgc cggcgaaaaa aacacttggt ggctcaggcg gtttcaatgg
    17101 gtcattatgc tgatgacgct ggtgtcgtcc gtgctgatgc ttttttggca ccagatttgg
    17161 gttatccgtc cgctgcaggc gttaagggaa ggtgcggaac gcatcggtca gcggcatttc
    17221 gacatccctg ttcccgaaga cggtacgccc gaattcaaac aggtcgggcg gtgtttcaac
    17281 caaatggcgc tcaggctgaa gactttatac gatgatttgg aaggacaggt tgccgaacaa
    17341 acccacaacc ttgaaaagca aaaccgcaac ctgaccctgc tgtaccggac cacgcgcgac
    17401 ctgcatcaat cctatacgcc gcgacaggcg gcggaagaat ttttgaacca catcctgccc
    17461 gccgtcggcg cgcaatccgg caacatctgc ctggaaaacg gttccgacac agacatttcc
    17521 gtccatacgg ccgaacacgg caaaaagccc cctttggaaa aataccatga cgaaaccttc
    17581 cccatcgaat atcaaaacga gaaactgggc atgctttcgc tcggcttttc agacggcact
    17641 tccctcaccg gagacgaccg cacgctgctt caaaccctga ttcggcaatt gggcgtatcg
    17701 ctcgccgggg cgaaacagga ggaagaaaaa cgtctgcttg ccgtattgca ggaacgcagc
    17761 ctgattgcac aggggctgca cgacagcatc gcgcaggcat taaccttcct gaacctgcag
    17821 gtacagatgc tggaaaccgc ctttgccgaa aacaaacggg aggaagccgc agaaaacatc
    17881 ggctttatca aaacaggcgt gcaggaatgt tatgaagatg tccgcgaact gctgctcaat
    17941 ttccgtacca aaatcagcaa taaagaattt cccgaagctg ttgccgacct gttcgcgcgc
    18001 ttcacgcaac aaaccgggat aacggtcgaa accgtttggg aaaacggttc gttcctgcct
    18061 acacaggacg agcagctcca aatgattttc atcctgcaag aaagcctgtc caacatccgc
    18121 aaacacgccc gcgccaccca tgtaaaattc accctttccg aatacggcgg acgctttacc
    18181 atgaccatcc aagacaacgg acaaggtttc gacacggaga aaataggaga acctacgggc
    18241 agccatgtcg gactgcacat catgcaggag cgtgccaaac gcatccgtgc cgttttagaa
    18301 atccgttccc aagcacaaca gggaaccacc gtctcattga cgggcgcacc caaagaaagc
    18361 ctaccatgac catcaaaatc atcctgatag acgaccacac cctcttccgc agcggcatca
    18421 aagccctttt gtcgcgccaa cacggttttg aagtcatcgg cgaagccgca gacggactct
    18481 cgggcatcaa aatgatcagt cggctgcaac ccgatgtcgt cctgctcgat ttggatatgc
    18541 caggcatgaa cgggcgcgag gccttgtccc aaatcatcag catcaatccg cagcaggcag
    18601 tcattatgct gaccgtttcc gaagacagcg acgaccttac cgaatgtatg cgcatcggcg
    18661 cgcgcggcta cctgctgaaa aacatcaacg ccgattttct tctcgaaagc atacgcaaag
    18721 ccgccgaagg cgataatgta ttctcgcccg agatgaccgc caaactcgtc aaaagcctga
    18781 tttcccccca acctgcccaa aggacgcagg cactctcctc acttacccct cgtgaactgg
    18841 aaatcttggg ctatctcgcc gcaggacaca gcaacaaaat catcgcccgc cacctcgatc
    18901 ttgccgaatc caccgtcaaa gtccacgttc aaaacctgct ccgcaaactc aacctcagca
    18961 gccgggtgca ggccgccgtt tacgccatcc ggcacaacgt cccccaacct gtgccggaat
    19021 agacgttcag acggcatcgg gtcaggaaaa aagcaatgcg cccctttgac ggggtgcaat
    19081 atataaggga cggcacaccg gatgccgtcc ctttaagtac gccattaccg ccgtaacccg
    19141 gacggaatgg cttaggcagt ttatttccaa gaatatttca cacccacggt gccttggtag
    19201 cctttatgtc tgttgcttcc gcatcctgcc gataattgcc ttgcctgtcc cgagaaaggg
    19261 tttgattttc gccacggcgt tggtggagcg tatccagcat cgcatagcct gattccaagt
    19321 tttgacgtgg aacgagtgcg taggccggtg cgcttggcgt gatgatggtt ttatcttgat
    19381 tcggcgtggt taaagtccag aagtattctg tcgcgcccgc ctcatctttg tgtgaaacca
    19441 attgcaattc ccccgcccct gtggttttgg cagttccgct aaaagtatcg cgcattgtgt
    19501 aggtatgttt agcgtcttcg atatttaatt taccggtttc cgcccccttg tcttgtccca
    19561 aaaccttaac aacggcggca ctgcgtttgt agcggtcgga aagttcgccg atcgagccgt
    19621 cgataatatt ttctttgcca tctttaccca ctgaaatgac cgttgttttt cctgaagcat
    19681 cgcctgtgat ttccagtaaa tcagattgag aatttcctcc ttgatcatca cccggcgagt
    19741 tccattctgt attgactttt aacacaccgt ctttaccgac ataatttcct tcaatggtga
    19801 gtttgtctgc ataggattga ttggcaagcg taatgatgcc gccgttggtg aatgttcctt
    19861 tttcaaatac agaagttgta cccgataaag aaatacctgt attagatagg gttagcgtgc
    19921 cgcctgataa taatgtagtc ccttcggggg ttgttaatga tgtgtctttt tttaggtgaa
    19981 aagtagcttt tgttagtttt atggttccaa tatcttctga tttgaattcc attgcgccat
    20041 ttgtaatttt tagcacagca ttggtaatag ggagggggaa gcgcattgta aatgattttt
    20101 ctgttccatc tggattgatt aatgtaccag atgcgccaaa gatctctcct tcagggatag
    20161 aaacagttcc atcagaaatt gatggtttat ttgcgctttt atcactgtgt ttatgaatta
    20221 atgtaat
//
LOCUS       DAWTCI010000031        20025 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWTCI010000031 DAWTCI010000000
VERSION     DAWTCI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20025
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.031"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            153..1217
                     /locus_tag="WM481_001112"
     CDS             153..1217
                     /locus_tag="WM481_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFA9080437.1"
                     /translation="MKAARFYNKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPGANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDKLVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1358..1681
                     /locus_tag="WM481_001113"
     CDS             1358..1681
                     /locus_tag="WM481_001113"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFA9080438.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELSRRQGLSGLAEKA
                     ARTES"
     gene            1755..2078
                     /locus_tag="WM481_001114"
     CDS             1755..2078
                     /locus_tag="WM481_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFA9080439.1"
                     /translation="MDNCIFCKIAAKEIPAQTVYEDGEMVCFKDINPAAPLHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKASGLTDGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2125..2328
                     /gene="tatA"
                     /locus_tag="WM481_001115"
     CDS             2125..2328
                     /gene="tatA"
                     /locus_tag="WM481_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFA9080440.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2332..3018
                     /gene="tatB"
                     /locus_tag="WM481_001116"
     CDS             2332..3018
                     /gene="tatB"
                     /locus_tag="WM481_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HFA9080441.1"
                     /translation="MFDFGLGELIFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQELDTQIELEELRKVKQAFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPLPDTANTVSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQAVEVSYIDTAVETPVPHTTSLRKQAINRKRDFRPKHR
                     AKPKLRVRKS"
     gene            3031..3801
                     /gene="tatC"
                     /locus_tag="WM481_001117"
     CDS             3031..3801
                     /gene="tatC"
                     /locus_tag="WM481_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFA9080442.1"
                     /translation="MSETQNEQTVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTAFEVPIVVILLTKIGAVTTEQLKHARPYVIVGAFVVAAVITPPDIIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPAKT"
     gene            3845..4453
                     /gene="maf"
                     /locus_tag="WM481_001118"
     CDS             3845..4453
                     /gene="maf"
                     /locus_tag="WM481_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9080443.1"
                     /translation="MNTLYLGSGSPRRMEILTQLGYRVVKLPAGIDETVKAGETPAPY
                     VQRMAEEKNQAALTLFCETNGAMPDFPLITADTCVFSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMEKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRPPLSALKP"
     gene            4462..4815
                     /locus_tag="WM481_001119"
     CDS             4462..4815
                     /locus_tag="WM481_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFA9080444.1"
                     /translation="MNRQTAYFLASFSLIALIALSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEKICAALSAVFSIIFFIS
                     CLSFVKQYKETNNVR"
     gene            4805..6619
                     /locus_tag="WM481_001120"
     CDS             4805..6619
                     /locus_tag="WM481_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HFA9080445.1"
                     /translation="MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWWLQARLMNLIYPGMNDI
                     ASWVFILLAVSAWACKSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWENTPL
                     LQNIIVHRGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNAILETF
                     TGIRYETAVERVANGGFTDLPRQSEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNFLSTLFTHSHNIILQLLAEMGISGTLLVAATLLTGIAGLLKRSLTPASLFLLC
                     ALAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     ELLHLDWTYTRLVNSFSPAADDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPETKPCK"
     gene            6809..7486
                     /locus_tag="WM481_001121"
     CDS             6809..7486
                     /locus_tag="WM481_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFA9080446.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7499..8905
                     /locus_tag="WM481_001122"
     CDS             7499..8905
                     /locus_tag="WM481_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9080447.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFKTRGDNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIE
                     RARLFAANNPHSNLVRIEYDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHE
                     FIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMAEKLEKLVAKERYLLHHVSHEMRSPLARMQAIVGLIQAQPQKREQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVAL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGFKTEKSAN"
     gene            9023..11446
                     /locus_tag="WM481_001123"
                     /pseudo
     CDS             9023..11446
                     /locus_tag="WM481_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
     gene            11491..11736
                     /gene="rpsP"
                     /locus_tag="WM481_001124"
     CDS             11491..11736
                     /gene="rpsP"
                     /locus_tag="WM481_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFA9080448.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAV"
     gene            11752..12261
                     /gene="rimM"
                     /locus_tag="WM481_001125"
     CDS             11752..12261
                     /gene="rimM"
                     /locus_tag="WM481_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFA9080449.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLA
                     KDGKTVSVTLEAGKVVNGELQVKFEGIDDRDSAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDDTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            12261..13010
                     /gene="trmD"
                     /locus_tag="WM481_001126"
     CDS             12261..13010
                     /gene="trmD"
                     /locus_tag="WM481_001126"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFA9080450.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELTHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGIL
                     GDIQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLNKILQEQREIQS"
     gene            13025..13390
                     /gene="rplS"
                     /locus_tag="WM481_001127"
     CDS             13025..13390
                     /gene="rplS"
                     /locus_tag="WM481_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFA9080451.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13711..14436
                     /locus_tag="WM481_001128"
     CDS             13711..14436
                     /locus_tag="WM481_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFA9080452.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTSQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TESYLAQASAKMQQQESPDWCAS"
     gene            14471..15346
                     /locus_tag="WM481_001129"
     CDS             14471..15346
                     /locus_tag="WM481_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFA9080453.1"
                     /translation="MNLYDLLLAPFAEFDFMHYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGVGGFAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVILISKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSGLTLITLAVIY
                     RPLVLESIDPLFLKSVNGKGGLWHVVFLILVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILPKWFKNHRHHTT"
     gene            15423..16337
                     /locus_tag="WM481_001130"
     CDS             15423..16337
                     /locus_tag="WM481_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFA9080454.1"
                     /translation="MKHLKLTLITALLATAATAAPLPVVTSFSILGDVAKQIGGERVA
                     VQSLVGANQDTHAYHMTSGDIKKIRSAKLVLLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEYDPHVWNDPVLMSDYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGNRYNISFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNVKALTNAMKQ"
     gene            16557..17423
                     /locus_tag="WM481_001131"
     CDS             16557..17423
                     /locus_tag="WM481_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFA9080455.1"
                     /translation="MKNILLVFVSFVPLCVRTDLPLNIEDIMTDKGKWKLETSLTYLN
                     SENSRAALASPVYIQTGSASFIPVPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGSG
                     SYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKAS
                     SGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAAND
                     RISLTGGIQWLGKQPDRIDGKKESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQS
                     SSELKLGIQHTF"
     gene            17466..18005
                     /locus_tag="WM481_001132"
     CDS             17466..18005
                     /locus_tag="WM481_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080456.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAGGLGAIPGDV
                     GAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQGI
                     GRHRPWESKSTDRSWKNRF"
     gene            17987..18352
                     /locus_tag="WM481_001133"
     CDS             17987..18352
                     /locus_tag="WM481_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080457.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMPSDDLFLKCLQSSYKAYSETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            18410..18787
                     /locus_tag="WM481_001134"
     CDS             18410..18787
                     /locus_tag="WM481_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080458.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGEWLKHIYEFNRESFIFSDVLLQKGESEENFSEIVLKSIKNN
                     KILTVEVRSGLHFDLRNIYRIEM"
     gene            18790..18987
                     /locus_tag="WM481_001135"
                     /pseudo
     CDS             18790..18987
                     /locus_tag="WM481_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19158..19693
                     /locus_tag="WM481_001136"
                     /pseudo
     CDS             19158..19693
                     /locus_tag="WM481_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5376 a         5924 c         4400 g         4325 t
ORIGIN      
        1 gagggaaggg aattttgcaa aggtctcagt ttgtagtaaa atatctaaac agattcaatc
       61 ggaaacggtc aggcttcttc acgccattcc catttcaacc gccgcctgct agattcagac
      121 ggcattccta cttgaaaaca aggagcgtta caatgaaagc agcacgtttt tacaacaaag
      181 gcgacatccg catcgaagac atccccgaac cgaccgtcgc ccccggcacc gtcggcatca
      241 atgtcgcctg gtgcggcatc tgcggtaccg acctgcacga atttatggaa ggcccgattt
      301 tcatcccgcc ctgcggtcat ccgcatccga tttccggcga gtccgcgccc gtaacgatgg
      361 gacacgagtt ctccggcgtg gtttatgccg tcggcgaagg cgtggacgac attaaagtcg
      421 gccaacacgt cgtggtcgaa ccctacatca tccgcgacga cgtaccgacc ggcgaaggca
      481 gcaactacca cctctccaaa gacatgaact ttatcggctt gggcggctgc ggcggcggcc
      541 tgtccgaaaa aatcgccgtc aaacgccgtt gggtgcatcc gatttccgac aaaatcccgt
      601 tggatcaagc tgctttgatt gaacctctgt ctgtcggaca ccacgcctat gtacgcagcg
      661 gcgcgaaagc aggcgatgtc gcattggtcg gcggcgcagg cccgatcggt ttgctgttgg
      721 ccgccgtgtt gaaagccaaa ggcatcaaag tcatcattac cgagttgagt aaagcacgca
      781 aagacaaagc ccgcgaatcc ggcgttgccg actacatcct cgacccgtcc gaagtcgatg
      841 ttgttgaaga agtgaaaaaa ctgaccaacg gcgaaggcgt ggacgtggcg ttcgaatgta
      901 ccagcgtgaa caaagtgttg gatactttgg tcgaagcctg caaacccggc gccaacttgg
      961 ttatcgtatc catctggagc caccccgcca ccgtcaacgt ccacagcgtc gtgatgaaag
     1021 aactggatgt gcgcggcacg attgcctact gcaacgacca cgcggaaacc atcaaactgg
     1081 tcgaagaagg caaaatcaac ctcgaaccct tcatcaccca gcgcatcaag ctggacaaac
     1141 tggtttccga aggcttcgag cgtctgatcc acaacaacga atccgccgtt aaaattattg
     1201 tgaatccaaa cctgtaagca aattacattt gcaacagcac aaatgccgtc tgaaacgctc
     1261 agacggcatt ttccaacgaa acgcaacagg cgtattgccc gagagcagcc gtttagccta
     1321 tcattccgaa caaacagaaa caaacagaag gaaaatcatg ggagattccg tactatccgc
     1381 catccaacaa accattatac agcgcaaatc tgccgacccg tccgaatctt acgtcgcaca
     1441 gctcctgcac aagggcgagg acaaaatcct aaaaaaagtg attgaagaag cgggcgaagt
     1501 gttgatggca tccaaagaca aagacccgtc ccacctggtt tacgaagttg ccgacttatg
     1561 gttccacacc atgattcttc tgacacacca cgacctgaag gcggaagacg tattggacga
     1621 actttcccgc cgtcaggggc tgtcggggct ggccgaaaaa gccgcccgta cagaatcctg
     1681 aatttatatt acaatctcat cccttccacg tttaatccgt ctgcgtgctt ttttcagacg
     1741 gcaacggagc cgttatggac aactgtattt tctgcaaaat cgccgccaaa gagattccgg
     1801 cgcaaaccgt ctatgaagac ggcgaaatgg tttgtttcaa agacatcaac cccgccgctc
     1861 cgcttcatct gctgctgatt cccaaagtcc attttgattc gttggcacac gccgcgcccg
     1921 aacatcagcc ccttttggga aaaatgatgc tgaaagttcc cgaaatcgcc aaagcttcgg
     1981 gactgaccga cggtttcaaa acccttatca ataccggcaa aggcggcgga caagaggtct
     2041 tccacctgca tatacacatc atgggcacac ccgtataaac cgttatttca caatcaaccc
     2101 ctaatactta cttaaggata catcatgggc agtttttctc tgacgcactg gattatcgta
     2161 ctgattatcg tcgttttgat attcggcacc aaaaaattgc gcaacgtcgg caaagacctc
     2221 ggcggtgcgg ttcatgactt caaacagggg ctgaacgaag gcacagacgg caaagaagcc
     2281 caaaaagacg atgtaatcga acacaaaaaa gacgaagaca aagcataact tatgtttgat
     2341 ttcggtttgg gcgagctgat ttttgtcggc attatcgccc tgattgtcct tggtccagaa
     2401 cgcctgcccg aagccgcccg cactgccgga cggcttatcg gcaggctgca acgctttgta
     2461 ggaagcgtca aacaagaact tgacactcaa atcgaactgg aagagctgag gaaggtcaag
     2521 caggcattcg aagctgccgc cgctcaggtt cgagacagcc tcaaagaaac cgatacggat
     2581 atgcagaaca gtctgcacga catttccgac ggtctgaagc cttgggaaaa actgcccgaa
     2641 cagcgcacgc ctgccgattt cggtgtcgat gaaaacggca atccccttcc cgatacggca
     2701 aacaccgtat cagacggcat ttccgacgtt atgccgtctg aacgttccga tacttccgcc
     2761 gaaacccttg gggacgacag gcaaaccggc agtacagccg aacctgcgga aaccgacaaa
     2821 gaccgcgcat ggcgggaata cctgactgct tctgccgccg cacctgtcgt acaggccgtc
     2881 gaagtcagct atatcgatac tgctgttgaa acgcctgttc cgcacaccac ttccctgcgc
     2941 aaacaggcaa taaaccgcaa acgcgatttt cgtccgaaac accgcgccaa accgaaattg
     3001 cgcgtccgta aatcataaag ggggcatccg gtgtccgaaa cacaaaacga acaaaccgtc
     3061 caaccgcttg tcgaacacct catcgagcta cgccgccgcc tgatgtggat ggttgtcggc
     3121 attctcgtct gctttttcgg catgatgccg tttgcccagc agctctatac ttttatcgcc
     3181 gacccgctga tggcaaacct gcccaaagac accagtatga ttgcgaccga tgtcatcgca
     3241 ccgtttttcg ttccggtcaa agttaccctg atggcggcgt ttttggtttc actgccgcat
     3301 acgctctacc aaatctgggc attcgtcgcc cccgcactct accaaaacga aaaacgcctg
     3361 attacgccgc tcgtcctctc cagcgtcagc ctgtttttca tcggcatggc atttgcctat
     3421 ttcctggttt tccccgtcat tttcaaattc cttgccggcg taacccccgt cggggtcaat
     3481 atggcgaccg acatagacaa atacctgtcc tttatcttag gtatgttcgt tgcgttcggc
     3541 acggcatttg aagtgcccat cgtcgtcatc ctgttaacca aaatcggcgc agtcacgacc
     3601 gagcagctca aacacgcccg gccttatgtc attgtcggcg cgtttgtcgt tgccgcagtc
     3661 atcacgccgc ccgacattat ttctcagacc ctgcttgcca ttccgctgat cctcttatac
     3721 gaggcgggca tttggttcgg acgcttcttc actccacgtt cagaacagga tggcgacata
     3781 cagccgcctg caaaaacctg acactatgcc gtctgaagcc ccgccgcaca ttaaggagca
     3841 caccgtgaac accctttact taggttcggg cagcccgcgc cggatggaaa tcctgacaca
     3901 gttgggctat cgggtcgtca agctgcccgc cggcatcgac gaaaccgtta aagccggcga
     3961 aacgcccgcc ccttacgtcc aaaggatggc ggaagaaaaa aatcaggcgg ctttgaccct
     4021 cttttgcgaa accaacggcg caatgcccga tttccccctg attaccgccg acacctgcgt
     4081 cttttcagac ggcatcatat tgggcaaacc tcgctcccaa gccgaagcaa tcgaattttt
     4141 aaaccgattg tccggcaaac aacataccgt cctgactgct gtctgcattc attatcgcgg
     4201 caaaacgtca agccgcgtcc aaaccaaccg cgtcgttttc aagcccctga gttcggaaga
     4261 aatttccgcc tatgtgcaaa gcggcgaacc gatggagaaa gccggtgcct acgccgtgca
     4321 aggcataggc ggtatcttta tccaatctat cgaaggcagc ttcagcggca ttatggggct
     4381 gcccgtttat gaaaccgttt ccatgttgca ggatttggga taccgccccc ccttatccgc
     4441 ccttaaaccg taaagtccgc cgtgaacaga caaaccgctt acttccttgc ctccttcagc
     4501 ctgattgcac tgattgccct atccctttct tgggaattat ggattgcacc gttacgcccg
     4561 ggcggttcgt ggctcgcgct caaagccctg cccctctgcc tgccgctttc aggtattcta
     4621 aagaaaaaaa tctataccta ccaatacagc tccatgctgg ttctgattta ctttgccgaa
     4681 gccgtcatgc gcctgttcga cgcctatccc gcagaaaaaa tttgcgccgc gctttccgca
     4741 gtattcagca tcatcttctt catatcctgc ctgtcgttcg tcaaacaata caaggaaaca
     4801 aacaatgtcc gctgaaacga ccgtatccgg cgcgcgcccc gccgccaaac tgccgattta
     4861 catcctgccc tgcttccttt ggataggcat catccccttt accttcgcac tcaggctgaa
     4921 accgtcgccc gacttttacc acgatgccgc cgccgcggcc ggcctgattg tcctgttgtt
     4981 cctcacggca ggaaaaaagc tgtttgatgt caaaatcccc gccatcagct tcctcctgtt
     5041 tgcaatggcg gcattttggt ggcttcaggc acgcctgatg aacctgattt atcccggaat
     5101 gaacgacatc gcctcttggg ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag
     5161 tttggtcgca cactacggac aagaacgcat cgttaccctg tttgcctggt cgctgcttat
     5221 cggctccctg cttcaatcct gcatcgtcgt catccagttt gccggctggg aaaacacccc
     5281 cctgcttcaa aacatcatcg ttcacagagg gcaaggcgta atcggacaca tcgggcagcg
     5341 caacaacctc ggacactacc tcatgtgggg catactcgcc tccgcctacc tcaacggaca
     5401 acgaaaaatc cccgcagccc tcggcgcaat ctgcctgatt atgcagaccg ccgttttagg
     5461 tttggtcaat tcgcgcacca tcttgaccta catagccgcc atcgccctca tccttccctt
     5521 ctggtatttc cgttcggaca aatccaacag acggacgatg ctcggcatag ccgcagccgt
     5581 attccttacc gcgctgttcc aattttccat gaacgccatt ctggaaacct ttacaggcat
     5641 ccgctacgaa actgccgtcg aacgcgtcgc caacggcggt ttcacagact tgccgcgcca
     5701 aagcgaatgg aataaagccc ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg
     5761 gaacagtttt gcccaacaaa ccttcctgat caatgccgaa cagcacacca tacacgacaa
     5821 cttcctcagc accttgttca cccattccca caacatcatc ctccaactcc ttgcagaaat
     5881 ggggatcagc ggcacgcttc tggttgccgc aaccctgctg acgggcattg ccgggctgct
     5941 gaaacgctcc ctgacccccg catcactttt cctgctgtgc gcgcttgccg tcagtatgtg
     6001 ccacagtatg ctcgaatatc ctttgtggta tgtctatttc ctcatcccct tcggactgat
     6061 gctctttctg tcccccgcag aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct
     6121 cggcatactg accgcctccg ccgccatatt cgcagaattg ctgcacttgg actggacata
     6181 cacccggctg gttaactcct tttcccccgc cgctgacgac agtgccaaaa ccctcaaccg
     6241 gaaaatcaac gaactgcgct atatttccgc aaacagcccg atgctgtcct tttatgccga
     6301 cttctccctc gtaaacttcg ccctgccgga ataccccgaa acccagactt gggcggaaga
     6361 agcaaccctc aaagcactaa aataccgccc ctactccgcc acctaccgca tcgccctcta
     6421 cttgatgcgg caaggcaaag ttgcagaagc aaaacaatgg atgcgggcaa cacagtccta
     6481 ttacccctac ctgatgcccc gatacgccga cgaaatccgc aaactgcccg tatgggcacc
     6541 gctgctgccc gaactgctca aagactgcaa agccttcgcc gccgctcccg gccatccgga
     6601 aacaaaaccc tgcaaatgac ccgcgtcggc ggatgcggat accgcccgaa atgtaaagct
     6661 ccatgcaaga cattgcaaaa aacagcaaac cggtagggaa atacgctatc aaaaaacatt
     6721 gcagccgtgt taagataaac tgtcaaacaa tcttttcacg ccacgcccga aacagggtcg
     6781 gggcataccc ttacgaaaag gaaacaccat gagccgcgta ttactcgtag atgacgatgc
     6841 cctgctgacc gaactgctga ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt
     6901 tcccgacggg gaagcaggcg tacaggaaat cctgagcggg caatacgatg tagtcgtatt
     6961 ggattccatg atgcccaaaa tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag
     7021 caccgtcccc atcatcatgc tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt
     7081 ggaaatgggt gcggacgact atgtccccaa accctgcaca ccacgcgaac tcttggcacg
     7141 catcaatgcc atcctccgcc gagcacaaca cagcggcgaa cagaacaacg cacccaacag
     7201 catctccgtc agcgatgtcg tcctataccc cgccaaacgc caggcatccg tcaaagacat
     7261 gccgctcgaa ctgaccagca ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg
     7321 acaggtagtc agcaaagaaa ccctgtccgt cgaagcactc gaccgcaaac tggcaaaatt
     7381 cgaccgcagt atcgacgtac acatctccag catccgccac aagctgggcg atgcctccct
     7441 gattcaaacc gtacgcggct tgggctacct gtttgtcaaa aactgaagca gcgggcaaat
     7501 gaaactgttc caacgcattt tcgccacatt ttgcgcggtt atcgtctgcg caatctttgt
     7561 ggcgagtttt tctttttggc tggtgcagaa cacccttgcc gaaaaccaat tcaaccaacg
     7621 ccgcaccatc gaaaccacat tgatgggcag cattatttcc gcattcaaga cacggggcga
     7681 caacggcgcg cgcgaaatcc tgaccgaatg gaaaaacagc cccgtctcat ccgccgttta
     7741 cgtcatacag ggcgacgaga aaaaagacat cttaaaccgc tatatcgaca attacaccat
     7801 agaacgcgcc cggttgtttg ccgccaacaa cccccattcc aaccttgtcc gcatcgaata
     7861 cgaccgtttc ggcgaagaat acctgttctt cattaaaggc tgggacaacc accaggcaca
     7921 acgcctgccc agcccgctgt ttatcccggg cctgccgctt gccccgattt ggcacgaatt
     7981 catcatcctc tccttcatca tcattgtcgg actgctgatg gcatatatcc ttgccggcaa
     8041 cattgccaaa cccatcagaa tcttaggcaa cggcatggac agggtggcaa acggagaact
     8101 tgaaacccgc atctcccaac aggtcgacga ccgcgacgac gaattgtccc atcttgccat
     8161 ccaattcgac aaaatggcag aaaaactcga aaaacttgtc gccaaagaac gttacctact
     8221 ccaccacgtt tcccacgaaa tgcgctcccc gcttgcccgt atgcaggcaa ttgtcggact
     8281 gattcaggca cagccccaaa aacgggagca atatctcaaa cggctggaag gcgaactgac
     8341 ccgcatggat acgctggccg gggaactgtt aaccctgtcc cgcctcgaaa cctccaatat
     8401 ggctttggaa aaagaaagcc tgaaactcct gcccttcctg ggcaacctgg tagaagacaa
     8461 ccaaagtatc gcccaaaaga acggacagac ggtcgccctg tctgccgacg gaaaaatccc
     8521 cgaaaacaca accatccttg ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat
     8581 ccgcaacgcc gtcaactaca gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga
     8641 ccacaaacac tggataatcg acgttaccga caacggcccc ggcgtggacg aaatgcagct
     8701 cccgcacatc ttcaccgctt tctaccgtgc agactccagc gccaacaaac ccggaacagg
     8761 actggggctt gcattgaccc aacatattat tgaacagcac tgcggcaaaa tcatcgccga
     8821 aaacatcaaa ccgaacggtc tgcggatgcg ctttatcctg cccaagaaaa aaaccggttt
     8881 caaaacagaa aaaagtgcga actgaccata atgccgtctg aacccgcttc agacggcatt
     8941 gcacaaacag ttaccccaaa acaaacacac agcgggtgca tctccaaaaa tcacgcacat
     9001 accgtccgca aaggaatata tcatgccggc acaaaccgat ccgggctact tcttcatgcc
     9061 caaccacatt atcctgatag gcgcgagcga acaaccgtac agcctgggtg aacgcgtact
     9121 cagcaacctg ctgagtacgc cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca
     9181 caccatagcc gggcttcccg cctacaccag cctcaacaaa atccccggca gtgcagacct
     9241 gattattacc gttaccccgc ccgacagtta cgacaccctc ctcaaaacct gccgtaaaaa
     9301 gcagctccga cacatcatcc tcatacagga ctgggacagc ctgtctgccg cagaactgca
     9361 caccgccgaa actgccatcc gcaaacacca cggcaacgga ctcaacatca ccgcctacac
     9421 caccgcaggc atccaactgc cctcactcgg actcaacatc agtacccaag acggatatgc
     9481 cgcaggccat accgccatac tgaccggcaa tgccgccgtc agccgccaaa tcgacaacat
     9541 cctgaacaaa ctccgtcaag gcacatcccg ccacatcaac ctgcatcccg gcatcagccc
     9601 catcacatcc gccgattggc tcaaccgctt cggacacagc ctgcacacca aaaccgccgt
     9661 cctacaccac aaccctgaag aggatcagcg caggctgttc agcgcaatcc gccaatttac
     9721 ccgccatacg ccgctgattc tccacatcac ctgcctcacg acagaaaccg accgtgccgt
     9781 actgcactgc ctcgcccgac actgcaactt cctcatcagt ttcaacgccg acgacctcga
     9841 agccgcactg agcgcccaac tgtccgacct tccacccctg tcccgactcg acatcctgtc
     9901 cgacacgcct gccgaatggc tgcacgcgca cgcaccaaaa aacctcaccc tccactttcc
     9961 caaccttccc caccacatcc gcaacggaca cctgaccggc acacccacac cctcaatctg
    10021 ccacgacatc gcctcacgtc agcttgcccg ccccgacacc caagccgtcc tgaccatcct
    10081 cggtccctcc ggacacgagg attacaaaaa aacagcacgc gcccttatcc gcctgtccga
    10141 acagaccgcc aaacccctgc tcgtcaacag ccccttttca gacggcataa cacatttcga
    10201 cacccccgca caggcaatcc gcaccctttc ctaccgtaac accgccgccg ccctgaaaca
    10261 ggcacagctc gacattgcac cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa
    10321 catcaaaaaa gcccttgcag cggcaaatcc cgccctgctc gccgaagccc tgcacctccc
    10381 cctaccggca caccacccat aacgccgtcc aattccaatt cggcagccac cccctctatg
    10441 gcgacatcct gaccgcacgc tgcaacggac aaaccactgc cgtactcccg ccgtttacca
    10501 cgctcgacag ccgccacctt gcccgctttg ccgaactcga cggcacacaa accctcgatc
    10561 agttcctgca cacactgacc gtcatccccg aataccgcca acacattttc ggcatcaccc
    10621 tcaacctcaa cggcggacaa tacagcagcg atttcatctt aaagacaccc ggaacacacg
    10681 acacgcccaa acgcaagaac acaggcaaag ccgcccaaac cctcgaacat gccgccgcaa
    10741 aaatgcagag tgccgccgca tacctggaac acaaaaaccc gacagccgcc gaatttctcc
    10801 gccacacaag cgaagccgcc gcagaactgc tcggcagcaa aaccgaaacc ggagcagccg
    10861 tacccaacgt acttgccccc tatcccgcag cacaccccaa aacactgtcc ctaaaaaaca
    10921 acacgaccgt taccattatc ccccttttac ccgaagacac agaagccaaa cagcagttcg
    10981 tccgcagcct cggccccgaa gcacggtaca cacgcttcat gacccacacc aacgaactgc
    11041 ccgcagccac gttggcacgc ctgtgcaacc ccgattacca ctgtgaaccg catggacggc
    11101 aagggatgcc gacagcaaca tcgtcgccgt cgtccgccac agccgcctga accgcaacga
    11161 atgcgaattt ggcatcacac tggcggaaca tatgcgcggc agcgggctgg cacagaaaat
    11221 gatggaactc atcatccaaa ccgccgcaca gcaaggctac ctgagcatga gtgccgacat
    11281 tctcaaaacc aataccccca tgatcaaact tgctgaaaaa tcaggattta ccctcaagga
    11341 atcggacacc gaaaaaaacc tgtaccgcgc atatctgaac cttgcggcag acaaaacaac
    11401 aggaaaaaca aataaaaact tgcgcaccgg ccacaaaata acctaaaatc ggcagtttcc
    11461 atatatccga ttttctcaaa aggactcaaa atggtagtta tccgtttggc acgcggcggc
    11521 tcgaaacacc gccccttcta caacgtcatc gttactgact cacgcagccg ccgcgacggc
    11581 cgcttcatcg aacgcgtagg cttctacaac cccgtagcca acgaaaaaca agagcgcgtc
    11641 cgcctcaatg cagaccgcct gaaccactgg attgcacaag gcgcgcaagt cagcgactcc
    11701 gttgcaaaac tgattaaaga acaaaaagcc gtctaatccg cgtttgccgc catgacagac
    11761 actcaaaacc gggtagccat gggctacatc aaaggcgtat tcggcataaa aggctggctg
    11821 aaaattgccg ccaacaccga atattccgac agccttttgg actaccccga gtggcatttg
    11881 gccaaggacg gcaaaaccgt cagcgttacc cttgaagccg gaaaagtcgt caacggcgaa
    11941 ctccaagtca aattcgaagg catagacgac cgcgattcag cattctcatt gcgcggttac
    12001 accatcgaaa taccccgtga agcattcgcc ccgacagaag aagacgaata ctactgggca
    12061 gacttggtcg gcatgaccgt cgtcaacaaa gacgataccg ttttaggcaa ggtaagcaac
    12121 ctgatggaaa ccggcgcaaa cgacgtattg atgattgacg gagaacacgg gcagattctg
    12181 attccgttcg tttcccaata tatcgaaacc gtcgataccg gcagcaagac cattactgcc
    12241 gactggggtt tggactactg atgcttatcc aggcagttac cattttcccc gaaatgttcg
    12301 acagcattac ccgctacggc gtaacgggac gcgcgaacag acagggaatc tggcagtttg
    12361 aagcagtcaa tccccgaaag tttgccgaca acagattggg ctatatcgac gaccgcccgt
    12421 tcggcggcgg tccgggaatg attatgatgg ctccgccgct tcatgcggcg atagaacacg
    12481 ccaaagcaca atcttcccaa accgcaaaag tcatctacct cagcccccaa ggaaaaccgc
    12541 tgacacacca aaaagcggca gaactggcag aacttacgca tctgattctg ctgtgcggac
    12601 gctatgaggg aatagacgaa agactgctgc aaagcagcgt cgatgaagaa atcagcatcg
    12661 gagacttcgt cgtttccggc ggagagcttc ccgccatgat gctgatggat gcggtattga
    12721 ggctcgtacc cggcatattg ggcgacattc agtctgccga acaggattcg ttctcaagcg
    12781 gtattttgga ctgcccccac tacaccaaac ccttagaatt tcaaggcatg gctgttccgg
    12841 aagtattgcg ctccggaaat catggcttga tagcggaatg gcggttggaa caatcgctgc
    12901 gccgcacctt ggagcgcaga cccgatcttt tggaaaagcg cgttttaatc ccaaaggaat
    12961 cccgcctctt gaataaaatc ctacaagagc aacgggaaat ccaatcataa tttaggaaaa
    13021 aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgcctga acaaagaaat
    13081 ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg aaggtacccg
    13141 cagccgtctg caagcctacg aaggcgtggt tatcgcccgt cgcaaccgtg gtttgaacag
    13201 caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt gaacgtactt tccaactata
    13261 ttcccctact gttgagaaaa tcgaagtcaa acgccgtggc gacgtacgcc gtgccaaact
    13321 gtactacctg cgcggtctga ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg
    13381 caaaggttga ttcaaaccgt tttcccccaa tgccgtctga acgttcagac ggcatttttt
    13441 attgccgcga tgccggcaag gcaacgatat gcctgaccct catccacctt aattaacaat
    13501 cactcccgcc tattcaagca gaatttacag actaaacgtt atacagtatc atatccgcca
    13561 tataacgata accgctttgc caacttccct ttcaaaactg caaatcatta tgacaccttc
    13621 cctcttactg tcaggattga ccttccgact cttcctcgcc ctgattgccg tattcctttt
    13681 atggggtgtt tacctttggg cggtatcggt atgagcatca tcgttgaaaa cctgacggtc
    13741 agctaccgcc gccgccccgc cgtacaccac gtcgacatta cttttgaaga acatagtatg
    13801 tgggcggttt tcggtcccaa cggcgcagga aaatctacct ttctcaaatc cttaatggga
    13861 ttgcagccta tcgatacggg cagcatccgg ctggacggat tgaccagtca aaacattgcc
    13921 tatctccccc aacaatccga catcgaccgt tcccagccta tgaccgtttt cgacttggcg
    13981 gcaatggggc tgtggtatga aatcggtttt ttcaaaggga taaataccgc tcaaaaacaa
    14041 cgcgttcacg aagccttgga gcgtgtcggt atgcggcagt ttgcccgccg ccaaatcgcc
    14101 cacctctcaa acggacaatt ccaacgcgtc cttttcgccc ggatgctggt tcaaaatgca
    14161 aaattcctgc ttttggacga acctttcaat gctgttgatg cacggacaac ctacgagctt
    14221 ctcgacgtgt tgcaaaaatg ccattgcggc ggacacgcta tcattgccgt tttgcacgat
    14281 tacgaacaag tccgtgccta ctttcccaat accctgctgc tcgcccgcga aaaaattgcg
    14341 gcaggcgcaa ccgagaccat tctgacagaa tcctacctcg cccaagccag cgccaaaatg
    14401 cagcaacagg aaagccccga ctggtgcgcc tcataaatac tgcccgaaac cacgccccac
    14461 accagacacc atgaacctct acgacctgct ccttgccccc tttgcagaat tcgacttcat
    14521 gcactacgcc ctcgcatccg tcttctgcct gtccctcagc gccgcacccg tcggcgtatt
    14581 cctcgtcatg cgccgtatga gcctgatagg cgacgcattg agccacgccg tcctgcccgg
    14641 tgccgccgtc ggctacatgt ttgccggctt gagcctgccc gctatgggtg tgggcgggtt
    14701 tgccgccggt atgctgatgg cgctgcttgc cggactcgtc agccgcttta ccaccctgaa
    14761 agaagatgcc aactttgccg ccttttacct gagcagcctc gccatcggcg taatcctcat
    14821 cagcaaaaac ggcagcagcg tcgatttact ccacctcctt ttcggatctg tgcttgccgt
    14881 cgatattccc gcactgcaac tcatcgccgc cgtctccggc ctcacgctca ttacccttgc
    14941 cgtcatctac cgccccctgg tgctagaaag catagacccc ctgttcctca agtccgtcaa
    15001 cggcaaaggc gggctttggc acgtcgtttt cctcatcctc gtcgttatga acctcgtatc
    15061 cggcttccaa gctctcggca ccctgatgtc ggtcggactt atgatgctgc ccgccattac
    15121 cgcccgctta tgggcaagaa atatggggac gctcattctg ttgtccgtcc tcatcgccct
    15181 tttttgcggt ttgatcgggc tgctcatttc ctaccacatc gaaatccctt ccggccccgc
    15241 catcatcctc tgttgcagcg tcctttatct cttttccgtc atactcggca aagaaggcgg
    15301 catcttgccc aaatggttca aaaaccaccg ccaccacacc acctgacccc gcaagccgct
    15361 tgaacaaaca ggcggcttgt tttaccataa tgaatgttat gttatatcac agaagaacag
    15421 ttatgaaaca cctcaaactc acccttatta ccgcattgct ggccaccgcc gcaactgccg
    15481 caccccttcc ggttgtaacc agtttcagca ttttaggcga cgtagccaaa caaatcggcg
    15541 gtgagcgcgt agccgtacaa agcctcgtcg gagccaacca agatactcat gcctatcaca
    15601 tgaccagtgg cgacattaaa aaaatccgca gtgcaaaact cgtcctgctc aacggcttgg
    15661 gacttgaagc cgccgacatc caacgcgccg tcaaacagag caaagtatcc tatgccgaag
    15721 cgaccaaagg catccaaccc ctcaaagccg aagaagaagg cggacaccat cacgaccacg
    15781 atcatgacca cgaaggacac caccacgacc acggcgaata tgacccccac gtctggaacg
    15841 accctgttct tatgtccgac tatgcccaaa acgtcgctga agccctgata aaggccgatc
    15901 ccgaaggcaa agtttattat caacaacgct tgggcaacta ccaaatgcag cttaaaaaac
    15961 tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc cgccaaacgc aaagtcctga
    16021 ccgggcacga cgcattttcc tacatgggca accgctacaa catcagcttc atcgccccgc
    16081 aaggcgtgag cagcgaagcc gagccgtccg ccaaacaagt cgccgccatc atccggcaaa
    16141 tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat caaagacacc cgcatggttg
    16201 accgcatcgc caaagaaacc ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg
    16261 gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca caacgtcaaa gccttgacca
    16321 acgcgatgaa gcaataaaac cgtttgattg gtttgaattt gaaaaggcaa aaggccgtct
    16381 gaaaattaaa tacgaggttt tcagacggcc ttctcttgtg caataaagca gtaaacaaat
    16441 attgataaga aaaaaacaat cagtattgaa aatatcgtta aaaatattat ttagacagat
    16501 tacatggaat ttcattaaca tcagtagact ttgatttcaa tcaagaaaac aatgtcatga
    16561 aaaatattct cttagtattt gttagctttg tgccattatg tgtccgcact gatctgccgc
    16621 tgaatatcga agacataatg accgacaagg gaaaatggaa actggaaact tcccttacct
    16681 acctgaacag cgaaaacagc cgcgccgcac ttgcctcacc ggtttacatt cagaccggct
    16741 ccgcttcctt tatccccgtc ccgaccgaaa ttcaggaaaa cggcagcaat accgatatgc
    16801 tcgccggcac gctcggtttg cgctacggac tgaccggcaa taccgacatt tacggcagcg
    16861 gcagctatct gtggcacgaa gaacgcaaac tcgacggcaa cggcaaaacc cgcaacaaac
    16921 ggatgtccga catatccgcc ggcatcagcc acaccttcct taaagacggc aaaaatcccg
    16981 cactcatcgc tttcctcgaa agcacggttt acgaaaaatc gcgcaacaaa gcctcgtcgg
    17041 gaaaatcgtg gctcatcggc gccaccacct acaaagccat agatccgatt gtcctttccc
    17101 tcaccgccgc ctaccgcatc aacggcagca aaaccctttc agacgacgtc aaatacaaag
    17161 caggcaatta ctggatgctg aatcccaaca tctcatttgc cgccaacgac agaatcagcc
    17221 tgaccggagg catccaatgg ctgggcaaac agcccgaccg catagacggc aaaaaagaat
    17281 ccgcaagaaa cacatccacc tacgcccatt tcggcgcagg tttcggtttc accaaaaccg
    17341 cggctttaaa cgcatccgca cgtttcaacg tttcagggca aagcagttcc gaactgaaat
    17401 tgggcataca gcatacattt taagcagatt ttgattttcc aaccttgtaa tccaaaggag
    17461 ttattatgaa aaaacaaatc accgcagccg taatgatgct gtctatgatc gcccccgcaa
    17521 tggcaaacgg attggacaat caggcatttg aagaccaagt gttccacacg cgggcagatg
    17581 cgccgatgca gttggcggag ctttctcaga aggagatgaa ggagactgaa ggggcttttc
    17641 ttccattggc tatcttgggt ggtgctgcca ttggtatgtg gacacagcat ggttttagtt
    17701 atgcaacgac aggcagacca gcttctgtta gagatgttgc tggcggatta ggcgcaattc
    17761 ctggtgatgt aggtgctgca ggaaaggttg tttcctttgc taaatatgga cgtgagatta
    17821 aaatcggcaa taatatgcgg atagcccctt tcggtaatag aacaggtcat cctattggaa
    17881 aatttcccca ttatcatcgt cgagttacgg ataatacggg caagactttg cctggacagg
    17941 gaattggtcg tcatcgccct tgggaatcaa aatctacgga cagatcatgg aaaaaccgct
    18001 tctaactcct cctttttttc tatttgaaga tgtgtctttg gatatatttc aaggattgtc
    18061 ggaattggaa aagaaaatag agccgcagga tttgatggat gatgtttacc gtgcttttga
    18121 ttcggttgga aatattctga atttccgtat agtagaaaaa gagcaaaaag ggttttgggt
    18181 aagtaccaaa atcaaaacag ttgtatttga ttcggcagat atgccttctg atgatctttt
    18241 tctgaaatgc ttacagtctt catataaggc ttattctgaa actgaacctg ccggtttaga
    18301 taaacggcag ctgatgaaaa cgttaattca aaaatgcgga ttcagttgtt gacgaaaaaa
    18361 tggattctga actaacagaa actgatttcg accatgtatg aggtaaaaaa tgtctttcgg
    18421 ctatttgatt gcaacatcgc aaccttgcga attgttgaca aaatctcgag gtgaaacttt
    18481 ttctttaatt atggataaga tggatttatg gatttatttc agattttgtg aaggaaatat
    18541 ttatacaata aggaagaatg aaactgaatc ttgccttaca gaaagaggag gtgaatggtt
    18601 gaaacatatt tatgaattta atcgggaaag ttttattttt tctgatgtgc ttctacaaaa
    18661 gggagaaagc gaagagaatt tttcagaaat tgtcttaaaa tcaataaaaa ataataaaat
    18721 tttgacagtt gaggtacggt caggcttgca ttttgactta agaaatattt atcgtattga
    18781 gatgtaataa tgcaattgat atgtgccgat tggacaggta taggaaattt tatgaaaacc
    18841 ttcgaaaaaa catggtctgc acaatatcgg gatatggaaa tttcagtacg gaatttttgg
    18901 aatttgaaac ggacaggggc ggaagtctat atcaacggca ggcgggttta tcataacgaa
    18961 gccgggatgg cgtctgcttc tttgcgttcg ctaatggggg aatacctgga atttgaagaa
    19021 agcggtacga aaattaccgt tgaaatcggc agcgcgtggc attttaatga aactataaga
    19081 aatattaata cacatttaag aggtacgaaa cttccaggtt ctaactggag agttcaagat
    19141 aaggggcatt ttcatctttg gaaaagataa taaattctaa ggtaagggag actaatttta
    19201 atcaggcatg gcgtgttata cgcgccatgc aaataaataa tatatctgat gaacaggtta
    19261 tgattgtcaa aatgtcttgt tatcttaaga aagatgaaat ttttaagaat atagaaagat
    19321 atggatatca gtcatcagat attaagaaaa ttaatcctcc ttttccatta aatattttac
    19381 ctgttcccaa accttattat ttttatcttt atgataccca tttgaatcaa atttatgatt
    19441 tggcttggtc atgtaatgat tttttaatgt ttgaaatatt tattttggat agggaaagca
    19501 tcaaatatat agataaagat aaacttttta aacctggaga aacactatat ttgaatttta
    19561 aatctattaa aaatcaatgc aaaaattatt tttttgcggt tttggttttg atgcccctta
    19621 ctttaaaagc ggtgcgagtc aagtggcaga ttataatttt gttcctgatg ccttttcttt
    19681 attctttatt taatcaaatt atttgaataa gcgtagggtg ggcacttgct gcccgcgtgt
    19741 tctgtttcaa gtttattatt gggtgaatat ttggaatttg aagaaaacgg tacaaagatt
    19801 gctgcggaaa tcggcagtgc atggcatttt ttgggcgcgg cttgtcggat ttcaataaat
    19861 ggaaagtatt atgcgggtaa tcggattgtt tggtttgcca aaaaagccga aagctaggaa
    19921 taatttgaca agggcagcct gaaagatttt caggctgcct tttatgtgga aaatatgatg
    19981 aagttcaaat atgtttttct gttggcgtgt gttgccgttt cttta
//
LOCUS       DAWTCI010000032        19837 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWTCI010000032 DAWTCI010000000
VERSION     DAWTCI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19837
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.032"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            65..670
                     /locus_tag="WM481_001137"
     CDS             65..670
                     /locus_tag="WM481_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFA9080459.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDIDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAAANKEQAKAREAIGNGLTMLSPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            742..1134
                     /locus_tag="WM481_001138"
     CDS             742..1134
                     /locus_tag="WM481_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFA9080460.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLRIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPSENTSAETAPRPAKKGLKLVK"
     gene            1139..1285
                     /locus_tag="WM481_001139"
     CDS             1139..1285
                     /locus_tag="WM481_001139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080461.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNLFRRIETVLPLPIRYWDI
                     ENAV"
     gene            complement(1333..1779)
                     /locus_tag="WM481_001140"
     CDS             complement(1333..1779)
                     /locus_tag="WM481_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFA9080462.1"
                     /translation="MSLKTRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKENAEIDVLHRYLPQMLSAGEIRTA
                     VEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA"
     gene            complement(1911..2123)
                     /locus_tag="WM481_001141"
     CDS             complement(1911..2123)
                     /locus_tag="WM481_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFA9080463.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(2412..4262)
                     /locus_tag="WM481_001142"
     CDS             complement(2412..4262)
                     /locus_tag="WM481_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9080464.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQNNDAAAAAYLENAGDSAMAENVRNEWLKS
                     LGARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSPEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADTNSVLRMAEDGAIKRALVLFQHSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLAAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEILATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYASLFGAPHIPLKQRMGTVPAR"
     gene            4639..5376
                     /locus_tag="WM481_001143"
     CDS             4639..5376
                     /locus_tag="WM481_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFA9080465.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIQDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLSNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHAQSEIGRELIRED"
     gene            5378..6064
                     /locus_tag="WM481_001144"
     CDS             5378..6064
                     /locus_tag="WM481_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9080466.1"
                     /translation="MADLTFEQAVSTIVGMKDEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVLKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            6223..7089
                     /locus_tag="WM481_001145"
     CDS             6223..7089
                     /locus_tag="WM481_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080467.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASAAAPSADNGAAKK
                     EIVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(7152..7721)
                     /locus_tag="WM481_001146"
     CDS             complement(7152..7721)
                     /locus_tag="WM481_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFA9080468.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDVETHLVVSKGAEMAR
                     ASETDYTKDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSIAHTLSLFGIDTPDLAEWQGMAD"
     gene            7879..8739
                     /locus_tag="WM481_001147"
     CDS             7879..8739
                     /locus_tag="WM481_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFA9080469.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTAVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLSEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPESVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8809..9051
                     /locus_tag="WM481_001148"
     CDS             8809..9051
                     /locus_tag="WM481_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080470.1"
                     /translation="MTFFLLRVRIVFLFSFLIFEEIMNTRINFTATVGKRVSQCYVTS
                     VISTIGVTTSDAICSGGGTNKAKSQCNALLKAAGRC"
     gene            9414..9767
                     /locus_tag="WM481_001149"
     CDS             9414..9767
                     /locus_tag="WM481_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFA9080471.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFVPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            9757..10623
                     /gene="atpB"
                     /locus_tag="WM481_001150"
     CDS             9757..10623
                     /gene="atpB"
                     /locus_tag="WM481_001150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFA9080472.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHSEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRIVPTADLNTALALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            10680..10916
                     /gene="atpE"
                     /locus_tag="WM481_001151"
     CDS             10680..10916
                     /gene="atpE"
                     /locus_tag="WM481_001151"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFA9080473.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            10986..11456
                     /locus_tag="WM481_001152"
     CDS             10986..11456
                     /locus_tag="WM481_001152"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFA9080474.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKIAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLRDQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            11461..11994
                     /locus_tag="WM481_001153"
     CDS             11461..11994
                     /locus_tag="WM481_001153"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFA9080475.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ALLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            12005..13552
                     /locus_tag="WM481_001154"
     CDS             12005..13552
                     /locus_tag="WM481_001154"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFA9080476.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVSTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTLGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPVEKIAPGVIARKSVDQPMQTGLKAIDSMIPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            13577..14452
                     /gene="atpG"
                     /locus_tag="WM481_001155"
     CDS             13577..14452
                     /gene="atpG"
                     /locus_tag="WM481_001155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFA9080477.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDKIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKPPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            14490..15887
                     /gene="atpD"
                     /locus_tag="WM481_001156"
     CDS             14490..15887
                     /gene="atpD"
                     /locus_tag="WM481_001156"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFA9080478.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGSPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFSGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LAVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            15898..16320
                     /locus_tag="WM481_001157"
     CDS             15898..16320
                     /locus_tag="WM481_001157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFA9080479.1"
                     /translation="MSIMQVEVVSGEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGILEVQPDKVTVLADVAVRSAEMDRARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            16492..17388
                     /gene="glyQ"
                     /locus_tag="WM481_001158"
     CDS             16492..17388
                     /gene="glyQ"
                     /locus_tag="WM481_001158"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFA9080480.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLFAEENADLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYIESREKLGFPLIKK"
     gene            17505..19568
                     /locus_tag="WM481_001159"
     CDS             17505..19568
                     /locus_tag="WM481_001159"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFA9080481.1"
                     /translation="MTTQTLLIELLTEELPPKALNNLGNHFAASVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGRQDVYAYEYVQTGKPLGGLLENIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNQTLGHRFLSDGEIIIENADSYAAQMRGQGKVVA
                     SFAGRKAAIQTALEGQARRLNATVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAGRAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPQGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     VFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALYAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAGQMNAVADI
                     ALLGE"
     gene            19632..>19837
                     /locus_tag="WM481_001160"
     CDS             19632..>19837
                     /locus_tag="WM481_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9080482.1"
                     /translation="MQPLVSVLICAYNVEKYFAQSLAAVVNQTWRNLDILIVDDGSTD
                     GTLAIAKDFQKRDSRIKILAQAQNS"
BASE COUNT         4693 a         5279 c         5218 g         4647 t
ORIGIN      
        1 ggtttaaatt taatccacta taaatcagcc gttttgcagg catcacacag gagcgacaat
       61 tattatgatg accctctatt ccggcattac ctgccccttc agccaccgct gccgcttcgt
      121 tttgtacgaa aaaggtatgg attttgaaat caaagacatc gatatttaca acaagcccga
      181 agacctcgcc gtcatgaacc cgtacaacca agtccccgtg ctggtcgagc gcgatttggt
      241 gctgcacgag tccaacatca tcaacgaata catcgacgaa cgcttccccc atccgcagct
      301 tatgcccggc gatcccgtta tgcgcggccg ggggcggctg gtgctgtacc gtatggaaaa
      361 agaattgttc aaccacgtcc aagttttgga aaaccccgcc gccgccaaca aggaacaggc
      421 aaaagcgcgc gaagccatcg gcaacggcct gacgatgctt tccccttcgt tcagcaaaag
      481 caaatacatc ctcggcgaag atttttctat gattgatgtt gccctggccc cgctgttgtg
      541 gcggctcgac cattacgatg tcaaactggg caaaagtgcc gcgccgctgc tcaaatacgc
      601 cgagcgtatc ttccagcgcg aagcctttat cgaagcactg acacccgccg aaaaagccat
      661 gcgcaaataa gtccgaaatg cttgcaaaac ccgccgtttt gcaggcattt tcacattcag
      721 gcacaccaca cggaacccat catgcccacc tcaaccaaac cctacatcct ccgcgcactt
      781 tgcgaatggt gcagcgacaa cagcctcacg ccgcacatcc ttgtctgggt aaacgagcat
      841 acgcgcgtcc ccatgcagta cgtccgcgac aacgaaatta tgctcaacat cggcgcaacc
      901 gccacgcaaa accttcgaat cgacaacgat tggatcagct tttccgcccg tttcggcgga
      961 caagcgcacg acatatggat acctgtcgga cacgtcctca gccttttcgc acgggagacc
     1021 ggagaaggta tggggtttga attggaggag taccgccccg acacgccgtc tgaaaacact
     1081 tctgccgaaa ccgcgccccg gcccgccaaa aaaggtttga aattggtcaa ataaatctat
     1141 gccgtctgaa cggaatcgtg tttcagacgg cattttgtcc gatggggcgc aaacggaatc
     1201 cgtttatcgg caaaacctgt ttcggcgtat cgaaaccgtg ttgcccctgc cgattcgata
     1261 ttgggacatt gaaaatgccg tctgaacctg cgatacgggc ttcagacggc gttttgtccg
     1321 atattcgggc aatcaggcgg tcagtacggt tttcaagatt ttgttgactt cgcccatatc
     1381 ggctttgccg gcgaggcggg ttttcaatac gaccatcact ttgcccatat ccgccatacc
     1441 tgccgcgccg gtttcggcaa cggctgcttc gacggcggtg cggatttcgc cggcggagag
     1501 catttgcggc aggtagcggt gcagcacgtc gatttcggcg ttttctttgt ctgccaaatc
     1561 ctgacggccg gcttcagtgt agattttcgc gccgtctttg cgctgtttga ccattttggt
     1621 caggatggcg gtgattttgg catcgtcggc ttcggtgcgt tcgtctactt caaactgttt
     1681 gacggcggca ttgatgaggc ggatggtgcc gagggaaact tgatctttgg cgcgcatcgc
     1741 ggttttcata tcttcggtaa ggcgggtttt caggctcatg atgtcctcgc tgggatgtcg
     1801 gatggaaacg gcgggtttcg atgccgtctg aaaagcaaaa cacaccgcag agcaggtctt
     1861 gcggtgtgtc ttcatatcac agggctgacc tgtaatctgt acttgaacgt ttagtacatt
     1921 ttgggcggca gctgttggct gcgcaggcgt ttttgcaggc gttttacggc tgccgctttt
     1981 ttgcgtttgc gttcggtagt cggtttttcg taggcttcgc gggcgcgcag ctcggtcagc
     2041 aggccggttt tttctacggc gcgtttgaaa cggcgcatag cgacttcaaa tggttcattc
     2101 tcttttacgc ggattgcagg cattttattt cctttaataa attcggttgt tccatctgcc
     2161 catcatatcg gtggaaaggg tagggcagac ggtgttgtag gttttccgta tcgctgtgcc
     2221 tttcggcggc gggatacgcg ccgtgacgga aacatcacct cctaacgggc cggcttgtgc
     2281 cgtgtcaagc tgcttgaaaa agtaggaaaa acaattttcg gattgtctta tatttatggt
     2341 tcgccgtcaa tgccgttcgg gataaaaatg ccgtctgaaa gaccgggcgg gtttcagacg
     2401 gcatcggcac gtcagcgtgc gggaacggtg cccatacgct gtttgagcgg gatgtgcggc
     2461 gcgccgaaga gggaggcgta gtaggcggca ttggtcatca cttttttgac atagtcgcgc
     2521 gtttcggaaa acgggatggt ttcggcatat accgcgcctt cgaggggtgt gtccgcctgc
     2581 catcggcgcg ctctgccggg gccggcgtta tagcctgcgg tggcgaggat ttcgttgttt
     2641 tgcaggcggc gtttggtgtc cgccatatac cacgtcccca tacggatatt gccgtcggcg
     2701 gtgtagagct gggcggcatc cataccgatt ttgccggcga tttcgcgcgc ggtggcaggc
     2761 ataacctgca tcagcccctg cgcgcctacg cgggattgcg cgcctataac gaagcggctt
     2821 tcctgacgaa tcaggccgta aacccaagcc ggatcgacat taacattttg cgcgtggcgg
     2881 attacggtgt ctttaaacgg cgaaatgtag cgcaaggtgt agttgagttt gcggtcggtg
     2941 cgttccgcgc tgttgaccgc catatcgtaa aaaccgtggt cgaacgcggt ttgcgcggcg
     3001 gccagcagct tgtcttcgtc aaagccgcgt gtggcaaaac gccattccgc ctgagcctga
     3061 cggcgcattt tcgcatcgcc ggcggttcgg ctgtgttgga acaacaccag tgcgcgctta
     3121 atcgcgccgt cttccgccat acggaggacg ctgtttgtgt cggcatcggg aacattgttg
     3181 cgcgtatcga ttttgcgccc caactcttcc cctgccagta cggcataaaa attcctgccc
     3241 gtcgctgccg cctgtttgta aagtttttcc gcttcttgcg tgttgcccgt tgcggcgcgg
     3301 ctgcgtgcca gccagtagag ccaggtcggg cttttttgca gtttttcggg catatgcgag
     3361 atcacggagg ccagctcgtc ccaacgtcgg gcgcgcaagg cggcgcgggc gtaccactcg
     3421 atttggtcgt cggtcagttg gcggcggtcg gcaaccttgc cgtaatagtc caaggcggca
     3481 ggcacattga ggctttgcga ctgataatgc cccaatacgc cccacgcgaa actgcgttgt
     3541 tcggggctta aaccgctttc catttcggac agcagggcgg cggcattcgg cgatttgcgt
     3601 gcttctttgc cgatgacgtt caacagggca tattcgcgcg aaccttgtgt accgccgtca
     3661 aacgggctgc ccaatgcggc ggcaaggttg cgtgcgtctg tggtttggcg gccggccagc
     3721 agtccgcgca cgcgcctcca ggcatcgttg ccgtccaaca agccggatgc ggctgcctgt
     3781 tccaacagtt tggtgcagcc cgaaggcagt ttgcccgtat ttttaactag ttcggcggca
     3841 cgcgtatagt cgttgcggct cgaatcggcg tagcattcga cttcttgggc gcgcccttca
     3901 ggtttgagtt tggcatattc ctgtgcaaac agcgtccact gtctgcgcgc gcccaaagac
     3961 ttcagccact cgttgcggac attttccgcc atcgcgctgt cgccggcgtt ttccaaatag
     4021 gcggcggctg ccgcatcgtt gttctgtttc actgcatcca gtgcggacgg gtagccgccg
     4081 taatctgcca gcgtttttcc ttccggttcg gcagggcggg tgggaacgct tgccgaaagg
     4141 tcggcagttt ctatattgtc tgccggggtc ttgccggctg gcagtgtgtt tgtcgaagaa
     4201 cacgcggcaa gcaccagggc cgccagcagc ggcagggaat gcttcataga gggtaggtac
     4261 atcggatttc cttaagaatc ggaaccctga acggtcaggg ttggaaaaga caaaatgccg
     4321 tctgaacagg cgtttgcccg aattatatgc cgaaactgca ccgtctttgg aatgtttccg
     4381 acataattta tatttttcaa tcatttgccg tttgggtgcg aaccgctgcc tttgcccgtt
     4441 tcagacggca ttgtccgaaa tggttgcccg cttcctgcct tattgacaaa aaaacgcttt
     4501 cccgataata tcctacgaaa attaacctgc cgatttgaca cagcttgcgg gcataacagc
     4561 taaagcgttc cgacaatttc agctttatct tccgcgcccg ttgtgtccga catcgggctt
     4621 tgttgtatgg gaaagacaat gattattttg gacaaggttt ccaagcatta ccaaacgcgc
     4681 gacaaaaccc gttttgccgc cgtcgagccg accagcctcg aaattcaaga cggcgaaatc
     4741 ttcgggctga tgggctattc cggcgcgggc aaatccactt tgttgcgcct gatcaacctg
     4801 ttggaacgcc ccgacacggg caaagtcaac gtctgcggac aagaactgac cgcgctcgat
     4861 gccgccgcat tgcgtcaggc gcggcagaat atcggcatgg tgtttcagca gttcaacctt
     4921 ttaagcaacc gcaccgtcgc cggcaacgtt gcctttccac tggaaatcgc cggatggccg
     4981 tctgaaaaaa tcaaagcgcg cgttgccgag tgcctcgaaa tcgtcggctt gaccgaacgc
     5041 gccggccact accccgccca gctttcaggc gggcagaaac aacgcgtcgg aatcgcccgc
     5101 gcgctcgcgc ccaagccgca agtcatcctc gccgacgaac ccacttccgc cctcgacccc
     5161 gccaccacgc gcagtgtctt ggaatgtttg gaagacatca acaaacgctt caacgttacc
     5221 atcgtcatcg taacccacga aatgagcgtc atcagacgat tgtgcgaccg cgccgccctg
     5281 ttggataaag ggaaagtggt ggaaatcgtc gaagtacgcg gcaaccaaat ccacgctcaa
     5341 tccgaaatcg ggcgcgaact gattcgggag gactgatatg gcagacttaa cattcgaaca
     5401 agccgtttcc accatcgtcg gcatgaaaga cgaaatcgtc cgcgccttgg gcgaaacttt
     5461 cgtgatggtc ggcttgtcca ccacattcgc cgtcatcttc ggcacgctct tgggcgtgtt
     5521 gctcttcgta acttccagcc gccaactgca ttacaacaag ccggtgaact tcctgctcga
     5581 caacctcgtc aacctcatgc gcgccttccc cttcgtcatc ctgatgattg cgatgatacc
     5641 cgccacacgc gccatcgtcg gcagcaccat cggcccggtt gccgcctcgc tggtgttgag
     5701 cgtgtcggga ttgttttatt ttgcccgact ggtggaacaa aacctgcgcg aagtcccaaa
     5761 aggtgtgatt gaagccgccg ccgcgatggg tgcgccgccg attgccatcg tcctcaaagt
     5821 cctcttgaac gaagcgcgcg cgggcatggt ttccagcatt accgtgcttg ccatcgggct
     5881 tttgtcatac agcgcggcgg caggcatgat aggcggcggc ggcttgggcg acctcgccat
     5941 ccgctacggc tactaccgct accaaaccga agtcatcgtc ttcatcgtcg ccctcctcgt
     6001 gctgctggtc atcctgattc aaagcaccgg caacgcgttg gcgcggaaac tcgacaaacg
     6061 ttaaacccga atgccgtctg aacgccaaaa accccgccgc tatccgaaaa atgctataaa
     6121 atccccctgt tcgcggcaaa tgccgtctga acgccgaatc cggacggcag gactccctgc
     6181 ctgtcatttt tgtttgaaac cgccacaaca tcaggagaaa atatgaaaac cttcttcaaa
     6241 accctttccg ccgccgcact cgcgctcatc ctcgcagcct gcggcggtca aaaagacagc
     6301 gcgcccgcag cctctgccgc cgccccttct gccgataacg gcgcggcgaa aaaagaaatc
     6361 gtcttcggca cgaccgtcgg cgacttcggc gatatggtca aagaacaaat ccaagccgag
     6421 ctggagaaaa aaggctacac cgtcaaattg gtcgaattta ccgactatgt gcgcccgaat
     6481 ctggcattgg cggagggcga gttggacatc aacgtcttcc aacacaaacc ctatcttgac
     6541 gatttcaaaa aagaacacaa cctggacatc accgaagcct tccaagtgcc gaccgcgcct
     6601 ttgggactgt atccgggcaa actgaaatcg ctggaagaag tcaaagacgg cagcaccgta
     6661 tccgcgccca acgacccgtc caacttcgca cgcgccttgg tgatgctgaa cgaactgggt
     6721 tggatcaaac tcaaagacgg catcaatccg ctgaccgcat ccaaagccga tatcgcggaa
     6781 aacctgaaaa acatcaaaat cgtcgagctt gaagccgcac aactgccgcg cagccgcgcc
     6841 gacgtggatt ttgccgtcgt caacggcaac tacgccataa gcagcggcat gaagctgacc
     6901 gaagccctgt tccaagagcc gagctttgcc tatgtcaact ggtctgccgt caaaaccgcc
     6961 gacaaagaca gccaatggct taaagacgta accgaggcct ataactccga cgcgttcaaa
     7021 gcctacgcgc acaaacgctt cgagggctac aaataccctg ccgcatggaa tgaaggcgca
     7081 gccaaataag gcagtcgtat aaaatgatgc cgtctgaacc gcattccgtg ttcagacggc
     7141 atttttgtcc tttaatccgc cattccctgc cattccgcca aatccggcgt atcgattccg
     7201 aacagcgaca gcgtgtgtgc gatactgtgc gccactatgt cgtccgccgt ctgcggcttg
     7261 cggtacatcg caggaacagg gggaaacacc acgccgccca tttccgttac ccgcttcata
     7321 ttgtccaaat gggcaaggtt cagcggcgtt tcgcgcacca tcagcaccag ccgccgcctt
     7381 tccttcaaaa ccacatccgc cgcacgcgtc aagagattgt cgccgaagcc gtgcgcgaca
     7441 gaggcaagcg tccgcatcga acagggtgcg accagcatcc cgtccgtttt aaacgtaccg
     7501 ctggcaatgc acgccccgat attgccgatc ggatggacga aatcagccaa ggcatatact
     7561 tcgtctttcg tataatccgt ttccgaagcg cgcgccatct ccgcgccttt cgataccaca
     7621 aggtgcgttt cgacatcttg cgcgcgcaaa agttccaaag ccttcacgcc gtattggaaa
     7681 ccgctcgccc cgctgatgcc gattatcaaa cgccgtacca tcatccgcct ttcccataaa
     7741 accgcctgca acggcaaacc ggctattata gtgaaaaaac agaaatccga taaacacgga
     7801 tacaaattgt cggcaacacc caatatccga taaaataccc gatttaacat cctatctgaa
     7861 taggcacggg agggcggtat ggcaaaagta aaaggcggat tggggcgcgg cttggattcg
     7921 ctgctcgcca acggtgcgga caacagcagc ggcgaccggc tgactgcggt tgcggttaaa
     7981 gatatccggc ccggccgcta tcaggcgcgc gtccaaatcg atgacgaagc cttgcaggaa
     8041 ctggcagatt cgattaaggc gcaaggcgtg atacagcccg tcatcgtgcg cgaacacgga
     8101 ctgtcccgat acgaactgat tgcaggcgaa cgccgttggc gcgccgctca gattgccggc
     8161 ctgtccgaaa tccccgccgt tatcaaaacc atcagcgacg aaaccgcatt ggcaatgggt
     8221 ttgatcgaaa acctccagcg cgaaaacctc aaccccatcg aagaagcaca aggcttgaaa
     8281 cgccttgccg acgagttcgg gctgacccac gaaaccatcg cccaagccgt cggcaaaagc
     8341 cgaagcgcga tttccaacag cctgcgcctt ttaagcctgc ccgaatccgt gcaggaaatg
     8401 ctttaccaac gccgcctcga aatggggcac gcccgcgcac tgctgaccct gcccgtcgtc
     8461 gaacagctcg aattggcgca aaaggccgtc aaaaacggct ggtcggtgcg cgaagtcgaa
     8521 cgccgcagcc aggccgccct tcaaaacaaa cgtcccgagc caaaaaaaac tgccgccgcc
     8581 gacatcggcc gcctgaatga tttgctgact gaaaaattgg gtgtcaacgc cgaaatcaag
     8641 accgccaacc ataaaaaagg caaaatcatc ctgcatttcg acacgcccga aaccttcgac
     8701 catatcttga agcagttggg catagattac cggccttaat tttgcgggat atgccgtttg
     8761 aaatatagag aatagctttc cagattttaa gtgggaaata taattctatt gacatttttt
     8821 ctgcttcgcg taagaatcgt tttcctgttt tcatttttaa ttttcgagga aattatgaac
     8881 acacgcatca actttaccgc aactgtgggt aagcgcgtga gccaatgcta tgttaccagt
     8941 gtaatcagca caatcggcgt taccacttcc gatgcaattt gttcgggagg cggaacgaac
     9001 aaagccaaaa gtcaatgcaa tgctttgctt aaagcggcag gccgctgcta atcctttatt
     9061 cggaaaaggt cgtctgaaaa tattttcaga cgacctttta tttattgagc aaactcccca
     9121 aactgccgac aatccgaccg ataaactact acaatttcgc gttgattttt tattatttcc
     9181 acgtaaagca aacaaatcac gtaagaacat taaaagtgta aagaaattgc ggacaaactc
     9241 cttaatatcc tgtaatcata tgtaatatgt gttagaatag cggagttgga ttttggggag
     9301 tcgctcgtct gtatcggcgg cgagtttcac aaagattaac atattgcttg acgtaataag
     9361 gcttctaaat tatattagcc gcgccccaaa cctgaggcgg aaacattccc tttatgaagc
     9421 agattatcat cctacaatcc gccgtattgt ccatatgcgc cgcagttgcc tttgccgtat
     9481 ggggttttgc cggattcctt tcggcggtcg ggggcggttt gtcctacctg cttcccacgt
     9541 ttgttgcagt tttacttcta aaacttttca ggggaaaccc cttcctgcaa agcaggatgt
     9601 tcgtcttcgg agagatttta aaagtagtgc tgtcgctgtt gtccatgctt gccgtatttg
     9661 caatatggca tcaatcgctg gtgttcgtcc cgtttttgat ggggctgctc ggtgtcagcc
     9721 atctggtttt tttagtattg ttgagagtga aagattatgg caggtgaaac cattaccgct
     9781 gccgactaca tcaagcacca cttgcagagc ttgaccagtt tgtcggatgt tactcagggt
     9841 cagggactga aaaacattgc tgatttttcg tttattaacc ttgatgccgt tttttttgcc
     9901 gtcctgttgg gcgtaatcgg cagcttcctg ttgtggcgcg gtgcaaaaaa agcaacggca
     9961 ggcgttcccg gacgtttcca ggccgccgtg gaaatcttgt tcgagtttgt ggacgatatg
    10021 tgtaagagca tcattcacag cgaaaagtct agaaaagccg tcgcgccgtt gggtctgacg
    10081 ctgtttgttt ggattttcct gatgaacgcg atggatatgc tgccggtcga tttgctgccg
    10141 atggtatggc agggcattac cggcaaccat cacgccctgc tgcgcatcgt accgactgcc
    10201 gatttgaaca ccgctttggc actcgctgtc ggcgtgttgc tgatttgtat ctattacaac
    10261 atcaaaatca aaggattggg cggctggttt cacgagttgt tcagtgcgcc gttcggtgca
    10321 aaactcgcac ctgcgaactt tctgttgaac ttggtcgagt ttctttctaa aaccgtatcc
    10381 cacggtatgc ggttgttcgg caatatgtat gcgggcgagc tggtattcct gctgatagcc
    10441 ttgcttggcg gtgcttgggc ggcttccggc agcgttgaag tcatggatcc gattctgttt
    10501 gtattccaca ttattgccgg tttggcatgg gcgattttcc atattttggt gattaccctg
    10561 caggcattta ttttcatggc gttggcgttc gtctatatcg ggcaggcgca cgatgcccac
    10621 tgattttccc aagtagtttg tttttgtagt ttaacctttg tttctttaag gagtttaaaa
    10681 tgggtttgat tgctatcgca tgtggtttga tcgttgcatt gggtgcattg ggtgcatcta
    10741 tcggtatcgc aatggtcggt tctaaatatt tggagtcttc tgctcgccaa cctgaactga
    10801 ttggtccgct gcaaaccaaa ctgttcctga ttgccggtct gattgatgcc gcattcttga
    10861 tcggtgtcgc cattgcacta ctgttcgcct tcgtcaaccc gtttgcaggt gcataatcag
    10921 gacggattcg tccgttcgta caggtgcagc ctgtttgatt aaccccaata cgaaggttaa
    10981 gtaacgtgaa tatcaatgca acattattcg ctcaaatcat cgtctttttc ggtttggtat
    11041 ggtttaccat gaaatttgtg tggccgccga ttgcaaaagc tttggatgag cgtgccgcaa
    11101 aaatcgccga gggcttggct gccgccgagc gtggtaaaag cgatttcgag caggctgaaa
    11161 aaaaggttgc agaacttttg gcagaagggc gtaatcaggt ttccgaaatg gttgccaacg
    11221 ccgaaaaacg tgccgccaaa attgtcgaag aagccaaaga acaggcttct tccgaggcgg
    11281 cgcgcattgc agctcaggca aaggccgatg tggagcagga attgttccgc gcacgcgaat
    11341 ccctgcgcga tcaggttgcc gtgttggctg tcaaaggtgc cgaatctatt ttgcgcagcg
    11401 aagtcgatgc ttccaaacac gcaaaactgc tcgataccct gaaacaggag ttgtaatctt
    11461 atggcagagt ttgcaacgat cgccagacct tatgcaaagg cattgttcgg tctggctcag
    11521 gaaaaaaacc aaattgagtc ttggttgggc ggactggaaa aacttgcggc ggttgttcag
    11581 gaagggaagg tggctttatt gattgaccgt cctgaaacga atgcttcaga aaaagcagat
    11641 atcctcatcg atttggtcgg tttgaaagac aaggagttaa aaaactttgt tatcgtcttg
    11701 gccgggcaga aacgtttgtc gatattgccg gaagtgtatg ctcaatatca agacttgacc
    11761 ttatcattca accatatcaa atctgccgtc atttacagtg cctatccgtt gaccgacaaa
    11821 caggtcggcg agttggcgca aatgctgaat aagcgtttcg acagcgagct gaaaatctct
    11881 gtcgaaatcg aaccggagct gattggcggc ataaaagttg aagtgggtga tcaggttttg
    11941 gatttgtctg tacaaggcaa actgagtgct ttgtacacga ctatgacgaa ttaggagagt
    12001 tttcatgcag cttaatcctg ctgaaattag cgatctgatt aaagccaaga tcgaaaatct
    12061 gtctgtaaat gccgaagtga gtacccgtgg tacggtgatt tcggtaactg acggtatcgt
    12121 tcgcatccat ggtttgtcag atgcaatgca aggtgagatg ctcgaattcc cgggtaacac
    12181 tttaggcttg gcgatgaacc tggagcgcga ctccgtcggt gccgtagtgt tgggcgagta
    12241 cgaacatatt aaagaaggcg acacggttac ctgtaccggc cgtattttag aagtgccggt
    12301 cggacgcgaa ttggtcggac gcgtcgtcga tgcattgggt cggcctatcg acggcaaagg
    12361 cccgattaat acaactttga ccgcccctgt cgaaaaaatc gcaccgggcg tgattgcacg
    12421 caaatcggtt gatcagccga tgcaaaccgg tctgaaggcg attgactcta tgattcctgt
    12481 cggtcgcggt cagcgtgagt tgattattgg agaccgtcag acaggtaaaa cggccgtggc
    12541 attggatgcc atcgtcaacc aaaaaggtac gggtgttatc tgtatctatg tcgctatcgg
    12601 tcaaaaagca tcttctattg ccaacgtggt ccgcaaattg gaagagcatg gcgcgatgga
    12661 acacacgatt gtggttgctg caactgcatc tgaagcggcg gcattgcaat atatcgcacc
    12721 ttattcaggt tgtacgatgg gtgaattctt ccgcgatcgt ggcgaagatg ccttgattgt
    12781 ttatgacgat ttgtccaaac aggctgtggc ttaccgtcaa atttccctgc ttttgcgccg
    12841 tccgcccggc cgcgaagcct atcccggcga tgtgttctac ctgcactccc gtctgttgga
    12901 acgtgcggca cgtgtcaatg aacacgaagt ggaaaaattg accaacggcg aagtaaaagg
    12961 caaaaccggt tctctgaccg cgttgccgat tatcgaaacc caagcgggcg acgtatctgc
    13021 tttcgttccg actaacgtca tttcgattac cgacggtcag attttcttgg aaaccgacct
    13081 cttcaacgcc ggtatccgtc ctgcaatcaa tgccggtatt tccgtatccc gcgtaggcgg
    13141 tgctgcacaa accaaagtga ttaaaaagct gggtggcggt atccgtttgg cgttggcaca
    13201 atatcgtgaa ttggcggcgt tctcgcaatt tgcatccgat ttggatgaag ctacgcgcaa
    13261 acagttggag catggcgaag tcgtaaccga actgatgaaa cagaaacagt tcagtacgtt
    13321 gaatacggct gaaatggctt tgaccctttg ggcaatcaac aacggttcgt attctgatgt
    13381 tccggttgcc aaagctctag cttttgaatc tgaatttttg agctttgtcc gtacccaaca
    13441 tccggaagtt ttggaagccg tcaatgcttc aggtgcaatg tccgacgaga gcgagaaaac
    13501 gcttgaagca gccatgaaat ccttcaaatc ttcttacgcc tatcaggcat aaggcttgaa
    13561 gtgaaaggag tctgaaatgg cagtaggaaa agagattctc accaaaatcc gcagtgttca
    13621 gaatacccaa aagatcacta aagcgatgca aatggtgtca acctctaaaa tgcggaagac
    13681 tcaggaacgg atgcgtttgg cgcgtccgta tgccgaaaaa gtgcgtatgg tgatgagcca
    13741 tcttgcgcaa accaataccg atcatggtat tccgttactg gaatctcatc gggaaatcag
    13801 acgtgtcggt tttattttga ttacgtctga taagggtttg tgtggcggtt tgaacgccaa
    13861 cgtgctgaaa aagtttttgg cacaagttca agagtatcgg aatcaaggta ttgaagagga
    13921 aatcgtatgc cttggcagta aaggtctgat ggcgtgtcag agcattggtc tgaatgtggt
    13981 tgccagtgcc gtaaatttgg gcgatacccc aaaaatggaa atgctgctcg gacctttgac
    14041 agaacttttc caacggtatg agaaacatga aattgacaaa atccatctgg tgtattcggg
    14101 ttttgtcaat accatgcgtc aagaaccgag gatggaagta ttgctgccta tcggtgagaa
    14161 cgtgattggc gattcagctc ccaaaccacc gttcagctgg gaataccgct acgaaccgac
    14221 tgcacttgca gtgttggaat atctggttcg ccgctattta gagtctgtgg tttatcaggc
    14281 gttgagcgac aatatggcat ccgaacaggc agcccgcatg gttgccatga aagctgcaac
    14341 agacaatgca ggcaatgcca tcaaagagtt gcgtctggta tataacaaat cgcgtcaagc
    14401 tgcgattacc acggaattgt cagaaattgt agcaggtgcg gcggccgtct gatgccgtct
    14461 gaatcctgta cggaaattag gatacgataa tgagccaagg caaaatcgta caaattatcg
    14521 gtgcggttgt tgacgtggaa tttccacgcg acatgattcc gcgcgtttac gacgctttga
    14581 aattagacga aaacggtctg actttggaag tccaacagct tttgggtgat ggcgtagtcc
    14641 gtaccatcgc tatgggcagc tcggacggtt tgaaacgcgg catgactgtg agcaatactg
    14701 gttcgcccat tactgtgccg gtaggtaaag gtacgttggg gcgcattgtc gatgtattgg
    14761 gaactcctgt tgacgaggca ggtccaattg ataccgacaa gagtcgtgcc atccaccaag
    14821 ccgctcctaa gtttgacgaa ctgtcttcca caaccgaatt gctcgaaacg ggcattaaag
    14881 tgattgactt gctgtgtccg tttgccaaag gcggtaaagt aggtctgttc ggcggtgccg
    14941 gtgtgggtaa aaccgtgaac atgatggaat tgatcaacaa catcgccaaa gcgcacagcg
    15001 gcttgtccgt gttctcaggc gtgggtgagc gtacccgcga aggtaacgac ttctaccacg
    15061 agatgaaaga ttccaacgta ttggataaag tagccatggt gtatggccaa atgaacgaac
    15121 ctccaggcaa ccgtctgcgc gttgctttga ccggtttgac tatggctgaa tacttccgtg
    15181 acgaaaaaga cgaaaacggt aaaggtcgtg acgtattgtt cttcgttgac aacatctacc
    15241 gttacactct ggccggtacc gaagtatccg cactgttggg ccgtatgcct tctgcagtgg
    15301 gttaccaacc gacattggct gaagaaatgg gtcgtttgca agagcgtatt acctctaccc
    15361 aaaccggttc cattacttcc atccaagccg tatatgtacc tgcggatgac ttgactgacc
    15421 cgtctccggc gacaactttc gcccacttgg acgcgaccgt cgtattgagc cgtgatattg
    15481 cctctttggg tatttacccg gcagttgacc cgcttgactc tacttcgcgc caattggatc
    15541 cgatggtatt gggtcaagag cactacgacg tagcgcgcgg tgtacagtct actctgcaaa
    15601 aatacaaaga attgcgcgac atcatcgcca ttctgggtat ggacgaattg tctgacgaag
    15661 acaaactggc tgtaatgcgt gcccgtaaaa tccaacgctt cctgtctcaa ccgttccacg
    15721 ttgccgaagt gtttacaggt tctccaggca aatatgtcgc cctgcgcgat accattgccg
    15781 gcttcaaagc catcttgaac ggcgaatacg atcatctgcc cgaacaggca ttctatatgg
    15841 tcggcagcat cgaagaagcg gttgagaaag cgaaaacctt aaactgagga ggtcggcatg
    15901 agcatcatgc aagttgaggt ggtaagtggc gagcagaaaa tctattcagg cgaggcaaca
    15961 tttatcgttg ttccgactgt acagggcgaa ctcggtattt atccgcgaca cgagccgatt
    16021 atgagtttgg tgcggccggg ggctttgcgt ttgaccgttc cgggcgagga taaagaggtt
    16081 ttggttgctg tttccggcgg tattttggaa gtacagcctg ataaagtaac tgtcttggcg
    16141 gatgttgccg tccgcagtgc ggaaatggat cgggcacgtg cggaagaggc gaaaaaagcc
    16201 gcagaagcgg gcatttccca agctaaagac gataaggctt tggcggaagc acataaagca
    16261 ttggctgctg cgattgcgca gctcaaaact ttggactata tccgttcgca caagaaataa
    16321 acttgtttgg tttgaaaaag cacgaccgtt tggccgtgct ttttattttc cggatttaaa
    16381 ttaaaaaatt atccatcccc tggtttcaaa tcaggctgtc ttcgagataa atctattcaa
    16441 gcggctatgg aaacagtaaa ataaaacctt tattttatcg aacaataagc catgcttacc
    16501 ttccaacaaa tcatcttcaa actgcaaacc ttctgggcag acaaaggctg caccgtcatc
    16561 caacccttcg acatggaagt cggtgccggc acatcccatc ccgccacctg cctgcgcgcg
    16621 ctcggccccg agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc
    16681 cgctacggcg acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaaa
    16741 cccgcccccg ccaatatcca agacctctat ctcgactccc tgcgcgaatt gggcatcgat
    16801 cccaaagtcc acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggtgcg
    16861 tggggtttgg gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc
    16921 caacaagtcg gcggtatcga ttgcacgccc gtactcggcg aaattaccta cggcatcgaa
    16981 cgcttggcga tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg
    17041 ccggacggca ataccgtaag ctacggcgac gtgtaccatc aaaacgaagt cgagcaatct
    17101 acctacaact tcgaatacag cgatgccgac tggctgctgc gccagttcaa cgactacgaa
    17161 gcgcaagcca aacgcctgtt cgccgaagaa aacgccgacc ttgccttacc tgcctacgag
    17221 ctggtcctca aagcggggca tacgttcaac cttttagacg cacgcggcgc gatttccgta
    17281 accgagcggg caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa
    17341 tatatcgaaa gccgtgagaa actgggcttc ccgttgatta aaaaataacg atacgtcgtc
    17401 tgaagcaggt tttcaggcga cctttattgc gtatccatta atacaggccg tacggcatat
    17461 gcaggcttta taagcatggc ctacggacaa acagaaagtc tcacatgaca acccaaaccc
    17521 ttttaatcga actccttacc gaagaactcc cgccaaaagc cctgaataat ctgggcaacc
    17581 attttgccgc ttccgttgcc gaaggcttgg aaaaagcgca actggttgac ggcgcggccg
    17641 aatttacggc ttatgcctcg ccgcgccgtt tggcggttca agtcaaaaac gtgaaggccg
    17701 ttcaagccga tcagaaaatc gtgaaaaaag gccctgccgt ggcgaatgcc gtaaaagacg
    17761 gtgcgccgac caaggctttg gaaggttttg cgcgcggcgc aggcgcgaaa atcgaagacc
    17821 tgaccatcgt ccacgacggc aggcaggacg tgtacgccta tgaatacgtc caaaccggca
    17881 aaccgttggg cggacttttg gaaaacatta tcaatcaagc tgttaagaag ctgccgattc
    17941 cgaaagtaat gcgttggggc agcagcacgt ttaccttcgt gcgccctgtt cacgggctga
    18001 ttgtgctgca cggcggcgat gtcgtgaacg tcagcgtttt gggcctgcaa agcggcaatc
    18061 aaaccttggg acaccgcttt ctttccgacg gcgaaatcat cattgaaaac gccgacagct
    18121 acgccgcaca aatgcgcggg caaggcaaag tcgtcgcttc gtttgccggg cgcaaagccg
    18181 cgattcagac ggcattggaa gggcaggcac gccgtctgaa cgcgaccgtt gccgccgatg
    18241 aagccctgtt ggacgaagtg accgcgctgg tcgaatggcc tgttgttttg gaagccggtt
    18301 ttgaggaaca cttcctcgcc gtgcctcaag aatgcttgat tttgacgatg cagcaaaacc
    18361 aaaaatactt cccgctgctc gaccaaaacg gcaagctgat gaaccgcttc ctgctggttt
    18421 ccaacctgca aaccgaagac ccgtcgcaca tcatccgagg caacgaacgc gtattgcgcg
    18481 cgcgcctgtc tgatgccgag ttcttctaca aacaagacca aaaagcgacc ttggaaagcc
    18541 gcctgcccaa gctggcgaac gtggtgtacc acaacaaaat cggttcgcag gccgaacgca
    18601 tcgagcgtct gcaaagcatc gccgcccaca tcgccaaagc attgggcgcg gatgccgcgg
    18661 cagccgggcg cgccgcgcgt cttgccaaag ccgacttggt aactgaaatg gtcggcgagt
    18721 tccccgaact gcaaggcacg atgggcaaat actacgcccg cttggacggc gaaaccgaag
    18781 aaatcgccga agctattgag caacactacc aaccgcgttt tgccggcgac aagctgcccg
    18841 aaagcaaaat tgccgccgcc gtggcacttg ccgacaaact ggaaaccctg gtcggcattt
    18901 ggggcatcgg tttgattcct accggcgaca aagaccccta cgccctgcgc cgcgccgcct
    18961 tgggtatttt gcggatgctg atgcagtacg gtttggacgt gaacgaactg attcagacgg
    19021 cattcgacag cttcccgcaa ggtttgctca acgagaaaac gccgtctgaa accgccgact
    19081 ttatgcaggc gcgccttgcc gtgttgctgc aaaacgatta tccgcaagac atcgttgccg
    19141 ccgtactcgc caaacagccg cgccgtttgg acgatttgac cgccaaactg caagccgtcg
    19201 ccgtattcaa acaactgccc gaagccgccg cgctcgccgc cgccaacaaa cgcgtgcaaa
    19261 acctgctgaa aaaagccgat gccgagttgg gcgcggttaa cgaaagcctg ctgcaacagg
    19321 acgaagaaaa agccctgtac gctgccgcgc aaggtttgca gccgaaaatc gccgccgccg
    19381 tcgccgaagg caatttccaa actgctttgt ccgaactggc ttccgtcaag ccgcaggttg
    19441 atgccttctt cgacggcgtg atggtgatgg cggaagatgc cgccgtaaaa caaaaccgcc
    19501 tgaacctgct gaaccgcttg gcagggcaga tgaacgcggt ggccgacatc gcgcttttgg
    19561 gcgagtaacc gttgtacagt ccaaatgccg tctgaagcct tcaggcggca tcaaattatc
    19621 gggagagtaa attgcagcct ttagtcagcg tattgatttg cgcctacaac gtagaaaaat
    19681 attttgccca atcattagcc gccgtcgtga atcagacttg gcgcaacttg gatattttga
    19741 ttgtcgatga cggctcgaca gacggcacac ttgccattgc caaggatttt caaaagcggg
    19801 acagccgtat caaaatcctt gcacaagctc aaaattc
//
LOCUS       DAWTCI010000033        19299 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWTCI010000033 DAWTCI010000000
VERSION     DAWTCI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19299
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.033"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(78..1148)
                     /gene="lptG"
                     /locus_tag="WM481_001161"
     CDS             complement(78..1148)
                     /gene="lptG"
                     /locus_tag="WM481_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFA9080483.1"
                     /translation="MNLISRYIIRQMAVMAVCALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLASLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIAAVALGEWVAPTLSQKAENIKAAAINGKISTGHTGLWLKEKNSIINVR
                     GMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSIMGTDKIETSA
                     AAEETWPIAVRRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTQIYAIAWWRKLVYP
                     VAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGTPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="WM481_001162"
     CDS             complement(1145..2260)
                     /gene="lptF"
                     /locus_tag="WM481_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFA9080484.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAILIAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNNLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTISTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            2249..2431
                     /locus_tag="WM481_001163"
     CDS             2249..2431
                     /locus_tag="WM481_001163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080485.1"
                     /translation="MINHKIAVRGAVSGSAQADCPRKVSDGASKNGVQSCQPSSDGLL
                     SERPSENYRQPSQSGE"
     gene            2434..3840
                     /locus_tag="WM481_001164"
     CDS             2434..3840
                     /locus_tag="WM481_001164"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFA9080486.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCADKAPEHNTAAHALFSALEEGQ
                     NFSDTKIPTDNGLQAVAVVRLEKTDRAALNKAAAEAAKWAQNQETVNVDVHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFSQAVFHSAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLMNYLRNL"
     gene            3903..4343
                     /locus_tag="WM481_001165"
     CDS             3903..4343
                     /locus_tag="WM481_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFA9080487.1"
                     /translation="MPKVTFYTHVDQIPLFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSDTSVLLACGGNLPRIPEGMAVLNLSDGFWNTA
                     SVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            4603..5421
                     /locus_tag="WM481_001166"
     CDS             4603..5421
                     /locus_tag="WM481_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9080488.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDT
                     SSIGGTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGK
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEA
                     TFYIPSNLAYREQGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVN"
     gene            5528..6154
                     /locus_tag="WM481_001167"
     CDS             5528..6154
                     /locus_tag="WM481_001167"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFA9080489.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGQIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            6170..6841
                     /locus_tag="WM481_001168"
     CDS             6170..6841
                     /locus_tag="WM481_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFA9080490.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPAYYKDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVTYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QAAKP"
     gene            6959..7381
                     /locus_tag="WM481_001169"
     CDS             6959..7381
                     /locus_tag="WM481_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFA9080491.1"
                     /translation="MQVTSKWIDGMCFVGTAEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVIGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGAEK"
     gene            7904..8620
                     /locus_tag="WM481_001170"
     CDS             7904..8620
                     /locus_tag="WM481_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFA9080492.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRCEEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREKKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            8769..9671
                     /locus_tag="WM481_001171"
     CDS             8769..9671
                     /locus_tag="WM481_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFA9080493.1"
                     /translation="MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLGDMALTVLWLFVCTVGANLLALAVLGKLSPWRIGGKGKGVSVG
                     VSGSVRQLGCVLIGFVSGKLMCDIWMPSENAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     LNRRGIRLSVWFILSSLSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            9714..10488
                     /locus_tag="WM481_001172"
                     /pseudo
     CDS             9714..10488
                     /locus_tag="WM481_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            10600..12288
                     /locus_tag="WM481_001173"
     CDS             10600..12288
                     /locus_tag="WM481_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFA9080494.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     DYFDRLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMAAGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPALHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGVRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFQAGKTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCGEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRQDHGKDMPVFNRTVTLKDSWQPK"
     gene            12380..13336
                     /gene="gshB"
                     /locus_tag="WM481_001174"
     CDS             12380..13336
                     /gene="gshB"
                     /locus_tag="WM481_001174"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFA9080495.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     NGGLVTAQASAFEFSGAKNDDDHEWFKAADKVQTALKAFDAVIMRTDPPFDMQYLYST
                     QLLTLAEQQGAKVFNSGRAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IVKPLDGMGGMGIFRLTEKDPNIGSILETLMRFDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRSGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKSFDVAAMFADAVAAWSVR"
     gene            13495..13878
                     /locus_tag="WM481_001175"
     CDS             13495..13878
                     /locus_tag="WM481_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFA9080496.1"
                     /translation="MGPSSYAAEKKGKSGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNALLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLVAMLMLAAVWLSALFG"
     gene            14010..14483
                     /locus_tag="WM481_001176"
     CDS             14010..14483
                     /locus_tag="WM481_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFA9080497.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKMGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKTAPMPAMNHGHHHGEAHQH"
     gene            14608..15711
                     /gene="mnmA"
                     /locus_tag="WM481_001177"
     CDS             14608..15711
                     /gene="mnmA"
                     /locus_tag="WM481_001177"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFA9080498.1"
                     /translation="MNTTSNTSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEN
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTIGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELCYLDDETAELVFDEPQW
                     AVTPGQSAVLYDVDICLGGGIIQTTDKPVIITR"
     gene            15782..17452
                     /locus_tag="WM481_001178"
     CDS             15782..17452
                     /locus_tag="WM481_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFA9080499.1"
                     /translation="MEKIWLESYEKGVSAEIDITQYNSVSDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVARGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLIANPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVPSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDACGFLETGDIAVMDEKGRLK
                     LVDRKKDLVVVSGFNVYPNEIEEFIAHHEKVMEVACIGVPNEKTGEALKVFVVKKDPS
                     LTKEELTAFCRTGLTAYKVPKDIEFRDELPKSNVGKILRRELRQSAGK"
     gene            17564..19198
                     /locus_tag="WM481_001179"
     CDS             17564..19198
                     /locus_tag="WM481_001179"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFA9080500.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRKMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSVLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAVKIYGSGHIRERHRHRYEVNNNYVSALEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         4731 a         5176 c         5233 g         4159 t
ORIGIN      
        1 agggaacggc tatatgtctg cgggaatgtg tcaatgaaaa caaatgccgt ctgaagagag
       61 gttcggacgg catttgttca acgtttttcc tgtttgcgta tcagccaaac agcgagcaag
      121 gcgaaggcta tggtaggcag tgcgccggcg aggaagggtg gggtgccgta gagttggctg
      181 gtaaacccga agagcctgcc ggcaaggtgg aacagcaatc cgagacagat gccgccgaag
      241 agttttaagc ccatattgcc gtggcgcgtg gtttgcggcg taaaggcgaa ggcaacgagc
      301 gccatgaccc atgcggcgac ggggtaaacg agtttacgcc accatgcgat ggcgtagatt
      361 tgggtgtttt ggctgttgtt ttggaggtgg cggatgtagg tggtcagctc gccgacggac
      421 atttggtcgg gcttgacgag caatacgtcc atcaggttgc gtctgacggc aatcggccaa
      481 gtttcttcgg cggcggcgga tgtttcgatt ttgtctgtac ccatgatgct gcggcggatg
      541 tttttcaact gccagctgcc gtcgctgttc aaaacggcgg aatcggcttc cactgcctct
      601 gccaattcgt ttttatcgtt gcgcgcccaa attttgatgc ccaaaagcgt atggtcgggc
      661 aacattccgc gcacattgat aatgctgttt ttttctttca gccaaaggcc ggtatggccg
      721 gtgctgattt tgccgttaat ggcggcggct ttgatgtttt cggctttttg gctcagcgtg
      781 ggcgcaaccc attcgccgag cgcgacggcg gcaatagcaa aaataaaacc gaactgagac
      841 agaatcaaca gcagcttttt ggtgctcatg ccgctggctt tgatgacggc cagttcgctg
      901 ccggcggcaa gctggctgag agaggccagt ccgccgatga ggacggcgag gggcatgagt
      961 tcgtaggcgc gggcgggcat tttgagggcg gtgtagccca gcatttccca tatgccgtaa
     1021 ctgcctttgc cgaggttgcc ggtttcgtac aggatttcaa aaaagctgta caaagcgagg
     1081 aaggcaagga gcgcgcaaac cgccataacc gccatttggc ggatgatgta acgtgaaatc
     1141 aggttcattt tccgcctttc aatgtcagac ttttgccaac cgcctgccag aagggctggc
     1201 tgggcatact gcggacgcgc agaagtacga ttgcgatgac gaacatgatg atgtgcatag
     1261 gcagcagtcc gagccaaaaa tggattttgc cgtcttccac ggcttcaaaa agcagggtca
     1321 gcccgttttg gtaaattaaa aacaaaccga tggcaatcaa gatattgtag gtatgtccgc
     1381 tgcgcgggtt gaaataggaa agcggcacgg cgagtaggca gagcaggagg acgctgacgg
     1441 tcagcgagat gcgccacatc aattctgcct gatgttgcgg attgctgctg ccaatcagtt
     1501 gggcggtcga aatggtgcgg cggtgggaaa cggggtcgat aagtttgggc gtggtgctga
     1561 taatcaggtt gagtttttgg aaggaaacct gattgtagtc ggcgcgtccg ggcgtgccgc
     1621 tgtaacggta gccgtggcgc aattcgagcg tgcgtttgtt gtcgttcagc gagaagttac
     1681 cttctttggc gaagatgatg ttgtcgccgc cgtttttgtc ctgttcgcgc aggaacaggt
     1741 ttttcatgat gccggattcg gtgtcaaagg tttcgacgaa ataaaccctg ccgttgcgct
     1801 tgcccaagtt attgaactcg ccggcttcca ccaaagacaa ttcctgcttc tgcttcaaaa
     1861 tttcggcata ttcgcggctg cgcagttccg cccacggtat cacccaaagc tgcatgacgg
     1921 caatcaggat ggcaaacggc acggcaaact gcatgacggg gcgtatccac tgtttcaacg
     1981 ccaatccgca ggatagccag accgacattt cgctgtcgcg ccagtagcgg gtcaatacgg
     2041 tcagcgtgct gatgaatgcg gtcaacacca gcaaaagcgg ggtcataccg atgacccaga
     2101 agccgactaa ggccaacacg gcatcgatgg cgacacgccc gtcagctgcg cggccaagca
     2161 ggttgatcgc ctgcgtggac accaacaccg ccaagaggac gacgaaaatg ccgacggcgg
     2221 taaaagagag ttctttgatg aggtttcttt gataaatcat aaaatcgcgg tcaggggggc
     2281 ggtaagcggt tcggcgcagg cggattgtcc gcgcaaggtt tcagacggtg cttcaaaaaa
     2341 tggggtacaa tcgtgccagc cgtcttcaga cggtcttttg agcgaaaggc cgtctgaaaa
     2401 ctaccgacaa ccgtctcaat caggagaata aacgtggaat ttagcacaaa aaccgaaatc
     2461 ttgcaggaac agcaggcagg cgcgcagtta tttgtctgtg ccgacaaagc acccgagcac
     2521 aacaccgccg cacatgcgct cttctctgct ttggaagagg gtcagaattt ttccgacacc
     2581 aaaatcccga cggacaacgg tttgcaggca gtcgccgtcg tccgcctcga aaaaaccgac
     2641 cgcgccgcgc tgaacaaagc cgccgccgaa gccgccaaat gggcgcaaaa tcaagaaacg
     2701 gtcaatgtgg acgttcacgc ctttgatgag gcgcaagcag cagccgttgc cgaagctttt
     2761 gccatcgcgt tcggcaacgc tgcatatcgt ttcgaccgct acaaaaaaga agccaagccc
     2821 gccaaatttt cgcaagccgt gttccacagc gcacacgaag ccgccgtcaa agaagccctg
     2881 cgcgttgccg aagcacaagt ttacggacaa agcctctgcc gcgacttagg caatgccgca
     2941 ccgaacgaat gcacgcctga gttcctcgcg cgtaccgcca aagccgaagc cgagaaactg
     3001 ggcgcgcacg ccaaaatcat cgaaaaagac tacatcaaag aaaacatggg ttcgttttgg
     3061 tcggttgcca aaggcagcgt cgaagaccca tatttggttg aactgagcta tttcggtgcg
     3121 gccgacaaag aagccgcgcc tgtggtattg gtcggcaaag gcattacctt cgacaccggc
     3181 ggcatttccc tcaaacccgg cctgaacatg gacgaaatga agtttgacat gtgcggcgcg
     3241 gcaaccgtca tcagcacctt ctgcgccgcc gtcaaactgc aactgccgat caatctgatt
     3301 gccatcgtcg ccacttgtga aaacatgcct tccggcgcgg caaacaaacc gggcgatgtt
     3361 gtgaaaagta tgaaagggct gacgattgaa gtgttgaaca ccgatgccga aggccgtctg
     3421 attttgtgcg acgcgctcac ttacgccgag cagttcaaac ccaaagccgt catcgatgtc
     3481 gccaccctga ccggcgcgtg catcgtcgcc ttggggcacg atgtcagcgg cgtgatgggc
     3541 aataatcagg atttgatcga cagcctgctt gccgcttcct acaacgtgga cgacaaagcg
     3601 tggcaactgc cgctctttga aacctacaaa gaccagctca aatccaactt tgccgacatc
     3661 cccaatatcg gcacgcccgg cgcgggcacg attaccgccg caacattcct gtcctatttc
     3721 accgaaggct acccgtgggc gcaccttgac atcgcgggta cggcgtggaa atccggagcg
     3781 gaaaaaggtg cgaccggccg ccccgtaccg ctgctgatga actatctgcg gaatctttaa
     3841 ttttgccaca atgccgtctg aagcttttca gacggcatca tcctgtcaaa tgctcaaaca
     3901 atatgccgaa agttaccttt tatacccatg ttgaccaaat cccccttttt acctgtcggt
     3961 tgattgcgcg cgctatccga gacggcggca ggatactggt gtggtccgac tcgttcgggc
     4021 ggcttcagga attggacaaa atgctttggc aatacgaggc cgagagtttt attccgcacg
     4081 aaatttggga aacggaagaa gccatgccgt ctgacacatc cgtcctgctt gcctgcggcg
     4141 gcaatctgcc ccgaattccc gaaggcatgg ccgttttgaa cctgtccgac ggtttttgga
     4201 acaccgcttc ggtcttgccc gcgcgcgttt tggaaatcgt cggcaacagc ctggaagatc
     4261 tcgccgacgc gcgcgaacgc tttaccgcct accgccgaag cggttttgcc atcgaacatc
     4321 acggcatgga gggcaaggca tgaaatgccg tctgaaatgc cgcatttaca aaatttaaac
     4381 aggcttgatt ctatttgatt aaaatttcct tatcggttga accccgccac ttggacatct
     4441 gtccttcggg gcggtagaat caaaccttat tttggaaggt tcaatccctt ccaaaacagg
     4501 gcaacacaca gattgacgct ttatgtgcca tcctgtgtgt tgaaacattc aaactcggct
     4561 ataatcccgt ttttccgact ttatcgacag cgaagatcca tcatgaacac cattttcaaa
     4621 atcagcgcac tgaccctttc cgccgctttg gcgctttccg cctgcggcaa aaaagaagcc
     4681 gcccccgcat ctgcatccga acctgccgcc gcttctgccg cgcagggcga cacctcttca
     4741 atcggcggca cgatgcagca ggcaagctat gcgatgggcg tggacatcgg acgctccctg
     4801 aaacaaatga aggaacaggg cgcggaaatc gatttgaaag tctttaccga tgccatgcag
     4861 gcagtgtatg acggcaaaga aatcaaaatg accgaagagc aggcccagga agtgatgatg
     4921 aaattcctgc aggagcagca ggctaaagcc gtagaaaaac acaaggcgga tgcgaaggcc
     4981 aacaaagaaa aaggcgaagc cttcctgaag gaaaatgccg ccaaagacgg cgtgaagacc
     5041 actgcttccg gtctgcagta caaaatcacc aaacagggta aaggcaaaca gccgacaaaa
     5101 gacgacatcg ttaccgtgga atacgaaggc cgcctgattg acggtaccgt attcgacagc
     5161 agcaaagcca acggcggccc ggccaccttc cctttgagcc aagtgattcc gggttggacc
     5221 gaaggcgtac ggcttctgaa agaaggcggc gaagccacgt tctacatccc gtccaacctt
     5281 gcctaccgcg aacagggtgc gggcgaaaaa atcggtccga acgccacttt ggtatttgac
     5341 gtgaaactgg tcaaaatcgg cgcacccgaa aacgcgcccg ccaagcagcc ggatcaagtc
     5401 gacatcaaaa aagtaaatta agtccgaatc catgcccgaa acaggttttc gggcattttt
     5461 acggcaaatg ccgtctgaag ccaccaaaca gcggttcaga cggcatccct ttcaaaagcg
     5521 caccattatg aaaaacatcg tcatcctgat ttccggacgc ggcagcaata tgcaggcaat
     5581 cgtcaatgcc gccattccca acgtccgcat tgccgccgtg ttgagcaaca gcgaaacggc
     5641 tgccggtttg caatgggcgg ccgaacgcgg catcccgacc gacagcctga atcataaaaa
     5701 ctttgaatca cgacttgcct tcgatacggc catgatggaa aaaatcgacg catatcaacc
     5761 cgatttggtg gttttggcag gttttatgcg gattctgacc cctgagttct gcgcccatta
     5821 cgaaggcagg ctgatgaaca tccatccgtc catcctgccc tcgttcaccg ggcttcatac
     5881 gcacgaacgc gctttggaag caggttgccg cgttgccggc tgtacgattc atttcgttac
     5941 ggccgagctg gattgcggcc agattgtatc gcaagggatt gtgccgatac ttgacggcga
     6001 tacggcagac gatgttgccg cccgggtttt ggctgtcgaa cacaaactct atccgaaagc
     6061 cgttgccgat tttgccgccg gccgcctgat tatcgaggga aaccgtgtca gaaattcgga
     6121 aaacgccgat gccgcccgtt tcctgacggc gtaaaccggg cgggagcaaa tgatgaagac
     6181 ttttaaaaat atattttccg ccgccatttt gtccgccgcc ctgccgtgcg cgtatgcggc
     6241 aaggctaccc caatccgccg tgctgcacta ttccggcagc tacggcattc ccgccacgat
     6301 gacatttgaa cgcagcggca atgcttacaa aatcgtttcg acgattaaag tgccgctata
     6361 caatatccgt ttcgagtccg gcggtacggt tgtcggcaat accctgcacc ctgcctacta
     6421 taaagacata cgcaggggca aactgtatgc ggaagccaaa ttcgccgacg gcagcgtaac
     6481 ctacggcaaa gcgggcgaga gcaaaaccga gcaaagcccc aaggctatgg atttgttcac
     6541 gcttgcctgg cagttggcgg caaatgacgc gaaactcccc ccgggtctga aaatcaccaa
     6601 cggcaaaaaa ctttattccg tcggcggcct gaataaggcg ggtacgggaa aatacagcat
     6661 aggcggcgtg gaaaccgaag tcgtcaaata tcgggtgcgg cgcggcgacg atacggtaac
     6721 gtatttcttc gcaccgtccc tgaacaatat tccggcacaa atcggctata ccgacgacgg
     6781 caaaacctat acgctgaagc tcaaatcggt gcagatcaac ggacaggccg ccaaaccgta
     6841 aatcggtttt caggcggttt tttgccgttt tgtccatatt tctgtataag ccgcttgaaa
     6901 ttgtttacaa taacgccata tgcaaagcgg ttttaacgct attttcggga atgacaccat
     6961 gcaggttaca tcaaaatgga tagacggaat gtgttttgtc ggcacggcgg aaggcgggca
     7021 cagcgtcgtt atggagggat cggcggcaga aggcgcggcc aagcgcgggc ccagcccttt
     7081 ggaaatgctg ctgttggggg tggcgggctg ttcgagcatc gatgtggtga tgattgccga
     7141 aaaacagcgt cagaaagtga ctgactgccg tgcgacggtt acggcgaaac gggcggacga
     7201 tgcgccgcgc gtgtttaccg aaatccacat ccatttcaag gtaatcgggc atgatttgaa
     7261 agaatcggcc attgagcgcg ccgttcagat gtctgccgaa aaatactgtt cggcttcgat
     7321 tatgttgggc aaagcggcaa agattaccca cagttttgaa attgccgggg cggagaaata
     7381 ggcatcgggc ggcattatgg ggaaacggct gatgccgtct gaaggggaaa ggtgcggcag
     7441 gcaatccgga ggcgcataaa aaagcagata tattcggact gcacctcccg aatatatctg
     7501 cctgctgttt cctctttatt cagcctttat aatacttgga cttgtcgggg tattgtgcag
     7561 gcttgattcc ggattgtcaa caattttcgg tcaaatttta aatgccgcgt tttaaaatga
     7621 tgcccgcctg attttgcggg cgggcggaag gcgggaatcc cgttatttta agccatatat
     7681 taatttattg aattaaaata aatttgtgca atatagtgga ttaacaaaaa ccagtacggc
     7741 attacctcgc cttgccgtac tatttttgta ctgtctgcgg cttcgtcgcc ttgtactgat
     7801 ttaaatttaa tccactatac tttaatttct cctacacgat ttgaaacaca gccgtcttaa
     7861 aacatcttaa aaaatttgaa agcgtaaaaa tacggaaaac atcatgaact ttgaaaacga
     7921 cgacattatc catgcgccga ccacgtcttc cctgattctc gaagagcggc acgattcgga
     7981 gctgttccgt gtttacgccc tgattttgga cggcattacc gatcaggtgc tgctgcccgg
     8041 caaaaagctg accgaatccg aactttgccg tcagatggtg tgttcgcgca acaccgtccg
     8101 cggcgcgctg tcgcttttgg cgcacgacaa gattgtcgat ttgcaaccca acaggggcgc
     8161 gttcgtccac gttcccgatt tgaaagaaat gcaggatgtg ttcaatgcgc gcatcgaaat
     8221 ggagacgatg attttgaaca tcctcgccgg cctgccggat ttggaaacgc gcctcaagcc
     8281 gctttatgcg atgataaggt gcgaagaaga ggcctccggc aggggcgacc gcgtcggctg
     8341 gaaccgcctg tccaatgcct tccacgtcga actggcgcgc ctggtgggca acgatgtgct
     8401 gttcgacatt atgaacacgc tgtgcgcgcg gtcttccctg attgtcgccg tggcgggcgt
     8461 gcatcgcgag aaaaaacacg ccatcaatac gcatacgcat tccgaacacc gcgaaatcct
     8521 cgacctgctg ctggcgggca ggcgcaaccg cgtggtcaaa atcctgcgcc gccatttggg
     8581 caactgtatg gagcgtttgg aaaagacttt ggaagattga atgccggagc ggataagccg
     8641 cctgaacctt caggcggttt tttaatggcg aacctgatgc cgtctgaaat atggatgcgg
     8701 gtatctgcaa ttttcagacg gcaattttta agccgcacat atcatgcggc aataaaggag
     8761 ggtaggggat gagcagcctg atgacgttgt tttcggtatt ggtaccgatg tttgccggat
     8821 tttttatccg tgttcccaag ccttacctgc ccgcttcgga caaggtgctg tcggttttgg
     8881 tgtatgccgt gctgctgctg atcggcgtat cgttgtcgcg cgtggaggat ttgggttcgc
     8941 ggttgggcga tatggcgttg acggttctgt ggctgtttgt ttgtacggta ggggcgaacc
     9001 tgcttgcctt ggcagtgttg ggaaagttgt ccccgtggcg gataggggga aaagggaagg
     9061 gcgtttcggt cggcgtgtcg ggcagtgtga ggcagctcgg atgcgtactg atcggttttg
     9121 tgtccggcaa attgatgtgc gatatttgga tgccgtctga aaacgcgggt atgtactgcc
     9181 tgatgctgct ggtgttcctc atcggcgtac agctcaaaag tagcggcgta tcgttgcggc
     9241 aggttttgct taaccggcgg ggcatccggc tgtcggtttg gtttatattg tcatctcttt
     9301 caggcgggct gctgtttgcc gcatcggcag atggtgtgtc gtggacgaaa ggtttggcga
     9361 tggcttccgg cttcggttgg tattccctct cgggtttggt aatgaccgag gcttacgggg
     9421 cggtatgggg cagcatcatg ctgctgaacg atttggcacg agagctgttt gcactggcat
     9481 ttattccgct gctgatgaag cgttttccgg atgcggcggt gggggtcggc ggcgcgacca
     9541 gtatggattt cacattgccc gtaattcagg gtgcgggcgg tttggaagtc gtgccggtag
     9601 cggtcagctt cggcgtggtg gtcaatatcg ccgccccgtt tctgatggtg gtgttttcca
     9661 cgctgggctg aacgcggtaa aatcggcatc ccgatgcaag gaagcagaaa acgatgaaac
     9721 cgaaaatcca aaggcatgga gagattttaa gccttgtccg ccggcatcag tttatgtcgg
     9781 tggacgagct tgccgccgca ttggacgtta ccccgcagac gatacgttgc gacatccgcg
     9841 agttggagga gggcggcagc ctgaaacgcc atcacggcgg cgcatcttcg ggcggaaact
     9901 tgccggaggg tctgcccgcc gaccgccaaa cccggtgtca aaacgaaaaa aacgccattg
     9961 cccggctgat tgcggaacac atccccgacg gttcgtcgct gttcgtcagt atcggtacga
    10021 ccatggaagc cgcggcatca gagctggtga agcggcgcag cagcctgcgg attattacca
    10081 acaatatcca cgtcgcctcc gtcgtttcgg cacgtacgga ttacacggtc atcatcacat
    10141 ccggcgtcgt ccgccctttg gacggcggta ttaccggcgt ggcgaccgtc gactttatca
    10201 accagttcaa agtcgattat gccgtgatga gcacgcacgg cgtggaaagc gacggttcgc
    10261 ttttggatta cgattacaag gaagtcagcg tcatgcaggc gatgattgcc aacgcgcgcg
    10321 tccgttttct cggcgtggat cacagcaaat tccgcagcaa cgcgctggtc aggctcggcg
    10381 gcattaccgg cgtttgacaa agtatttacc gacaggttgc ctgataccgc gatgcagaag
    10441 atgctgaaag aggcgggggt ggaatgcctg attgccgatg ccgtctgaac gctatgtcaa
    10501 agcgcgcaag tcgggtacaa taaacacatc atcaaaccgc ttcagacggc atacggaacc
    10561 tccccaatgc cgtctgaagc catctgttta aagaaaacca tgctcaataa agaccaattc
    10621 gcggacaacc atttcatccg caccatcatc gaagacgacc tcaaaagcgg caaacacgaa
    10681 gccgtccaaa cccgttttcc gcccgaaccc aacggctatt tgcatatcgg acacgccaaa
    10741 tccatctgcc tgaacttcgg tttggcgtat atttacgacg gtttgtgcaa cctgcgtttc
    10801 gacgacacca acccggaaaa agaaaacgac gaatacgtca acgccatcaa agaagacgtc
    10861 gagtggctcg gtttccattg ggcgggcgag ccgcgtttcg cttccgacta tttcgaccgg
    10921 ctttatgact acgccgtcgg tttgattaaa gacggaaaag cgtatgtcga tgatttgaca
    10981 cccgaagaaa tgcgagaata ccgcggtacg ctgaccgaag cgggtaaaaa cagcccttac
    11041 cgcgaccgca gtatcgaaga aaacctcgac ctgttcacgc gtatgaaaaa cggcgagttc
    11101 cccgacggca gcaaaaccct gcgcctgaaa atcgacatgg cggcaggcaa catcaatatg
    11161 cgcgatcccg tcatctaccg catccgccgc gcccatcacc acaacaccgg cgacaaatgg
    11221 tgcatctacc cgatgtacga ttacacgcat tgcatttccg atgccatcga aggcatcacg
    11281 cattccttgt gcacgctcga atttgaagcg caccgtccgc tttacgactg ggtgttggac
    11341 aacatccccg cgctgcacgc cacccgtccg cgccaatacg agttttcccg tttggagctt
    11401 ttgtacacca ttacctccaa acggaaattg aatcagttgg ttgtggaaaa acacgtttcc
    11461 ggctgggatg atccgcgtat gccgaccatt tccggtatgc gccgccgcgg ctacacgccc
    11521 gaaggggtgc gcctgtttgc caaacgcgcc ggtatttcca aatctgaaaa catcgtcgac
    11581 atgagcgtgt tggagggtgc gattcgcgaa gagctggaaa actccgcccc gcgcctgatg
    11641 gcggttttga acccgctcaa agtgaccctg accaactttc aagccggcaa aacccaaagc
    11701 cgccgtgccg cgttccatcc gaaccacgaa gaaatgggcg atcgcgaagt acctgtttca
    11761 caaaccatct acatcgaagc cgacgacttt gccgaaaatc cgcccaaagg cttcaagcgt
    11821 ctgattcccg gcggcgaagt acgcttgcgc cacggctatg tcatcaagtg cggtgaagtc
    11881 gtcaaagacg aagcaggcaa tgtggttgaa ctcaaatgca gcatcgacca cgacaccttg
    11941 ggcaaaaatc cagaaggccg caaagttaaa ggcgtaattc actgggtttc cgccgaacac
    12001 gccgccgaaa tcaaagtccg tctgtacgac cgcctcttta ccgtcgagcg tcccgatgcc
    12061 gtgcgcggcg aagacggcga atacctgccg tttaccgatt tcctcaatcc ggaatccgtt
    12121 aaggaaatca ctgcttacgc cgaacctgcc gcgaaagatt tgccggcgga aagccgttgg
    12181 cagttcgagc gcatcggcta ttttgtgacc gaccgccaag accacggcaa agacatgccg
    12241 gtgtttaacc gcacggtgac gttgaaagat tcttggcagc ctaagtaaac cccatccttg
    12301 ccgtctgaat attgttcggg cggcatttct cctttacccg cgaaatgcgg cacactcggc
    12361 acaccggaaa ggaaatatga tgaaagtcct ctttatcgcc gacccgatgg caagtttcaa
    12421 aacctacaaa gacaccacct acgcgatgat gcgggaaatg gcaaaacgcg gctggcggct
    12481 gtttcatacc ttgagcgggg aattgtctgt aaacggcggt ttggtaacgg cacaggcatc
    12541 ggcatttgaa ttttcgggtg caaaaaacga tgatgaccat gaatggttta aagcggcgga
    12601 caaagttcag acggcattaa aagcatttga tgccgtgatt atgcgtaccg atccgccgtt
    12661 cgatatgcaa tacctttact ccacccaatt actgacgctg gcggaacagc agggcgcgaa
    12721 agtgttcaac agcggacggg cgatgcgcga ctttaacgaa aaactggcga ttttgaattt
    12781 cagccgcttt accgcgccca cgctggtaac gacccgttcc gccgatgtcc gcgcattttt
    12841 gaaagaacac ggcgacatca tcgtcaaacc gctcgacggc atgggcggca tgggcatctt
    12901 ccgcctgacc gaaaaagacc ccaacatcgg cagcatcctc gaaaccctga tgcggtttga
    12961 ttcccgcacc attatggcgc aacgctacat tcccgaaatc gtacacggtg acaaacgcat
    13021 cttgattatc ggcggcgaag tcgtccccta tgctttggcg cgtatcccgc aaaacggcga
    13081 aacacgcggc aatctggcgg caggcgggcg cggtgtggcg caggaattgg acggacgcga
    13141 ccgggaaatt gcagagactc tggctcccga gcttaaacgg agcggcatcc tgctggccgg
    13201 tttggacgtt atcggcagca acctgaccga agtcaacgta accagcccga ccggattcca
    13261 agaaattatg aaacaaaaaa gtttcgacgt ggcggcaatg tttgccgatg ccgttgccgc
    13321 gtggtcggta cgttaaaccg atgccgtctg aaaggctttt gcttcgtaac cgcttggttt
    13381 gttcgggcag gcagggtttt ccctcagccg gcagggatgc gcttaatgcc gtacggacac
    13441 ccctccgccc cgttttcaga cggcatatat tgaggacatt ttgaaaggat accgatggga
    13501 ccttcctcct acgcggcaga aaaaaaaggc aaaagcggca tcaggcgcgt catcaacgca
    13561 ttcggctatt cgatagacgg catcgccgcc gcctaccgtt acgaagcggc attccgtcag
    13621 gttttgtggc tgaacgcgct gctggtgtgc gcggcatttt tttgggtttc cgaaaccgcc
    13681 gtccgcctgc cgttgattat cgcgtctttt gtgtcggtca ttgtcgaact gttcaacacc
    13741 gccgtcgaag ccgccgtcga tcatacttcg accgaaaaac acgagctggc caaacgcgcc
    13801 aaagatgcag gttctgccgc acaactggtt gccatgctga tgttggcggc ggtttggctg
    13861 tccgccctgt tcgggtaaaa cgcttgcagc aggatttgta atcctttagg attagtattt
    13921 attattcact taatctacat caaatttccg agcagtattt gtatgtaaga ttaagcacat
    13981 tccccgtctg atattaaagg agcaggaaga tgaaaaaatt attggcagcc gtgatgatgg
    14041 caggtttggc aggcgcggtt tccgccgccg gagtccatgt cgaggacggc tgggcgcgca
    14101 ccactgtcga aggtatgaaa atgggcggcg cgttcatgaa aatccacaac gacgaagcca
    14161 aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc
    14221 acatcaacga caacggcgtg atgcgtatgc gcgaagtcaa aggcggcgtg cctttggagg
    14281 cgaaatccgt taccgaactc aaacccggca gctatcacgt gatgtttatg ggtttgaaaa
    14341 aacaactgaa agagggcgac aagattcccg ttaccctgaa atttaaaaac gccaaagcgc
    14401 aaaccgtcca actggaagtc aaaaccgcgc cgatgccggc aatgaaccac ggtcatcacc
    14461 acggcgaagc gcatcagcac taatctgctg gaaatatttg aaatgccgtc tgaaaaagcc
    14521 cgggctttca gacggcattt ttatgcccgc ctttaaaatg tgttaaaatc cgcctttaaa
    14581 aaccgccgtt tccaagccat cctgcgtatg aatacgacat caaacacttc caatatcatc
    14641 gtcgggcttt ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt
    14701 tatcaagtgc gcggtgtgtt catgcagaac tgggaaaacg acgacaacga cgaatattgc
    14761 agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattgtcgg catcgacatc
    14821 gacatcgtta atttcgccgc gcaatataaa gacaaagttt ttgcttattt tcttcaggaa
    14881 tacagtgcgg ggcgcacgcc gaatccggat gtgttgtgca atgccgaaat caaattcaaa
    14941 tgctttttgg actacgccgt agggcagggc gcggatacca ttgccaccgg acactatgcg
    15001 cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac
    15061 caaagctatt ttctctaccg cctcaagcct ttccaactcg aacgcgcgat ttttccgttg
    15121 ggcggtttgg aaaaacccga agtgcgccgc cttgccgccg aatttaattt gccgactgcc
    15181 gctaaaaaag acagtaccgg catctgtttc atcggcgagc gtccgttccg cgagtttctg
    15241 cagaaatact tgccgaccga caacggcaaa atggtgacgc ccgaagggaa aaccatcggc
    15301 gaacacgtcg ggctgatgtt ttacacattg ggtcagcgca aaggattggg catcggcggc
    15361 gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact catcgtcgta
    15421 caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc
    15481 acgctgcccg aacgtccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg
    15541 gacgcgcctt gcgaattgtg ctatttggat gatgaaaccg ccgagctggt gtttgacgaa
    15601 ccgcaatggg cggttacgcc gggtcagtcc gccgtgctgt acgacgtcga catctgtttg
    15661 ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata aaggtaatgc
    15721 cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa gaccgatacc
    15781 tatggaaaaa atctggttag aaagctacga gaagggcgtc agtgccgaaa tcgacatcac
    15841 gcaatacaat tccgtcagcg acgtattccg ccaaagcgtg gaaaaatttg cccgtctgcc
    15901 cgcttttcaa aatatgggca aaacgctcac ttatgccgaa accggcaaac tggcgaccga
    15961 tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg ttgccattat
    16021 gatgccgaac gtattgcaat atccgattgc ccttttcggt attttgcagg caggtttggt
    16081 ggcggtgaac accaatccgc tctatacgcc gcgcgagttg gagcatcagc tgaaagacag
    16141 cggtgcgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc tggtgctgcc
    16201 gcgcacgcag atcaaacacg tcatcgtcgc ctccgtcggc gaaatgttcg ggctgcttaa
    16261 aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg aataccgtat
    16321 tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg ttttccaacc
    16381 tgtcgcatta aaccgcgaag ataccgcatt gttgcaatac acgggcggca cgacaggcgt
    16441 tgccagaggc gcggtgctga gccacggcaa catctgcgcc aatatgcttc aggcaaaaga
    16501 atggattaaa aaccaattgc gcgagggaaa agaaaccgtt atcgccgccc tgccgctgta
    16561 ccacatcttc gccctgacag tgaatctgat gatttttgcc aatgccggct cgaaaattgt
    16621 cctgattgcc aacccgcgcg atatgaaagg ctttatcggc gaactgaaaa agcagcgggt
    16681 taacgtattt atcggcgtga acacgctgtt taacgcgatg gtcaaccggc ccgatttcgc
    16741 cgaagtcgat ttttcaggat tgcggctgac tttgggcggc ggtatggcga cccaaaaagc
    16801 cgttgccgaa aaatggaaaa aaatcaccgg cacgcccatc gtcgaagcct acggtttgac
    16861 cgaagccagc cccggcgtgt gctgcaaccc cttaaacatc gaatcataca gcggcagcat
    16921 cggtttgccc gtcccgtcca ccgaagtcga actgcgcgac gcaaacggca aagaagtccc
    16981 cgttgggcag ccgggcgaat tgtgggtaaa aggcccgcaa gtgatgcaag gctactggaa
    17041 ccgccccgaa gaaaccgcca aagccataga cgcgtgcggc tttttggaaa ccggcgatat
    17101 tgccgtgatg gacgaaaaag gccgtctgaa gctggtcgat cgcaaaaaag acctcgtcgt
    17161 cgtttccgga ttcaatgttt atccgaacga aatcgaggaa ttcatcgcgc accacgaaaa
    17221 agttatggaa gttgcgtgta tcggcgttcc gaacgaaaaa accggcgagg cactcaaagt
    17281 gttcgtcgtc aaaaaagacc cgtctttgac caaagaagaa ctcaccgctt tctgccgtac
    17341 cggtctgacc gcatacaaag tgccgaaaga catcgaattc cgcgacgagt tgcccaagtc
    17401 caatgtcggc aaaatcctgc gccgcgagtt gcgccaaagt gccgggaaat aaagaaaaga
    17461 taccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc cagaaccgcg
    17521 cgcttcacgt taaaatcacg cattccaaca tgggtattcc atcatgacca aattcatttt
    17581 cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt ctattgccgc
    17641 catcctcgaa tcgcgcggct tgaacgttac catgctcaag cttgatcctt atatcaacgt
    17701 cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg acgacggcgc
    17761 ggaaaccgac ctcgacttgg gacactacga acgtttcatc gattccacga tgacccgccg
    17821 caacagcttc agcacgggtc aggtgtacga aaacgttatc gccaaagaac gccgcggcga
    17881 ctatctcggc ggtacggttc aagtcatccc gcacattacc gacgaaatca aacgacgcat
    17941 ccacgaaggc gcggcgggtt acgatgtggc gattgtcgaa atcggcggta cggtcggcga
    18001 catcgaatcg ctgccgtttt tggaagccat ccgccagatg cgaagccagt tgggacgcaa
    18061 caacaccctg ttcgcccact tgagctacgt tccctacatc gccgccgcag gcgaaatcaa
    18121 aaccaagccg acccagcaca ccgtgaaaga aatgttgagc atcggcttgc aacccgacat
    18181 cctgatttgc cgtatggaca ggaaaatgcc ggcagacgaa cgccgcaaaa tcgccttgtt
    18241 ctgcaacgtg gaagagcgcg cgattgtcgg cagctacgat gtggacagca tctacgaatg
    18301 ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt tgcagcttaa
    18361 cgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccgtca aaaacccgaa
    18421 acacaccgtc aaaatcgcga tggtcggcaa atacgttgat ttgaccgaat cctacaaatc
    18481 attgattgaa gccttgaaac acgcgggcat ccataccgaa accgatgtgc agattacctt
    18541 cgttgacagc gaaagcatcg agaaaaacaa gggcgacgtt tccgtactca aagatatgga
    18601 tgccatcctc gttcccggcg gcttcggttc gcgcggcgtg gaaggcaaaa tcgccgccgt
    18661 gcgctacgcc cgtgaaaaca acgtgccata cttgggcatc tgcctcggta tgcagattgc
    18721 gctgattgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca ctgagtttga
    18781 cttgaaatgc gctgcccccg tcgtcgccct gattgacgaa tggcaaaccg ccgacggcag
    18841 cgtcgaaacc cgtgacgaat ccgccgattt gggcggcacg atgcgtttgg gcgcgcaaga
    18901 agtcgaattg aaagcaggca gcctcgccgt caaaatctac ggcagcggac acattcgcga
    18961 acgccaccgc caccgctacg aagtcaacaa caactacgtt tccgcgctgg aacaggcagg
    19021 tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca tcgaactgcc
    19081 gaaccatcct tggttcttcg cctgccagtt ccatcccgag ttcacgtcca acccgcgcaa
    19141 agggcatcct ttgttcaccg cgtttgtcaa agccgcgttg aacaataaaa aagcctgata
    19201 aagcgttact tgatgatcaa aatgccgtct gaaagcctga caaagacttt cagacggcat
    19261 tttcgcaaat cggggatcat aaaagccatc aatttaatt
//
LOCUS       DAWTCI010000034        18910 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWTCI010000034 DAWTCI010000000
VERSION     DAWTCI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18910
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.034"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            187..477
                     /locus_tag="WM481_001180"
     CDS             187..477
                     /locus_tag="WM481_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFA9080501.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKAGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            570..2204
                     /gene="groL"
                     /locus_tag="WM481_001181"
     CDS             570..2204
                     /gene="groL"
                     /locus_tag="WM481_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFA9080502.1"
                     /translation="MAAKDVQFGNEVRQKMVNGANILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTVID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIGMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGMGGMM"
     gene            2908..4443
                     /locus_tag="WM481_001182"
     CDS             2908..4443
                     /locus_tag="WM481_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFA9080503.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIIGGRLPELRRHLNALSSIRVGGLWTACVVFTVVMLGYMLYQ
                     DTAGLLEKNYGDYPDGFLNIFGWGMSAALIMFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     GEK"
     gene            4440..4532
                     /locus_tag="WM481_001183"
     CDS             4440..4532
                     /locus_tag="WM481_001183"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFA9080504.1"
                     /translation="MSTSAIVMMVAAIAVIWGGLLLSLLRLPEE"
     gene            complement(4568..5008)
                     /locus_tag="WM481_001184"
     CDS             complement(4568..5008)
                     /locus_tag="WM481_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080505.1"
                     /translation="MKHAPIILLLTASSAALLSAGCTALPSDRPPLRTLPKSAPIQPA
                     NPYSRPPSGTPDGAFSTRASNGRVLKSIVKNGVFDRFVDIYHPNGKLHSHTPVENGVA
                     QGYTEQGILRTRILYRGTGISSERKLWMRRARWNANGSRKTNAV"
     gene            complement(5202..6422)
                     /gene="lysA"
                     /locus_tag="WM481_001185"
     CDS             complement(5202..6422)
                     /gene="lysA"
                     /locus_tag="WM481_001185"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFA9080506.1"
                     /translation="MTLFCEQVAYPRLAEEFGTPLYVYSQSALTGAFENYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKIAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYKDEGVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNTRNRAAEVLVDGGGYKLIRRR
                     ETLEQQMANELACL"
     gene            complement(6433..6603)
                     /locus_tag="WM481_001186"
     CDS             complement(6433..6603)
                     /locus_tag="WM481_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFA9080507.1"
                     /translation="MKYGVFFAAATALLLSACGYKGGLYLPKEGDTARFGAIQTGLQL
                     QGKPQSAPPTQK"
     gene            6674..6997
                     /gene="cyaY"
                     /locus_tag="WM481_001187"
     CDS             6674..6997
                     /gene="cyaY"
                     /locus_tag="WM481_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFA9080508.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIAEL"
     gene            7030..7449
                     /locus_tag="WM481_001188"
     CDS             7030..7449
                     /locus_tag="WM481_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFA9080509.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGNTGY
                     FYAASQEGILDALHIYNVEDVSDKHIPNHVLILWDGACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSRKPT"
     gene            7471..7977
                     /gene="luxS"
                     /locus_tag="WM481_001189"
     CDS             7471..7977
                     /gene="luxS"
                     /locus_tag="WM481_001189"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFA9080510.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGAGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNRNGDLA
                     LDESLLNA"
     gene            8121..10913
                     /gene="polA"
                     /locus_tag="WM481_001190"
     CDS             8121..10913
                     /gene="polA"
                     /locus_tag="WM481_001190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFA9080511.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALIGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAP
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLAVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEIPSEKRAEKATAPEKLDYQA
                     VTTEAQFAALLDKLAKADTIGIDTETTSLDAMNAELVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNNIAGDAMLASYI
                     IESHLGHGLDELSGRWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEARLRAQMDDKQLEMYEKMELPVAQVLFEMECNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPRDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPPGSVIVSADYSQIELRIMAHLSGDKTLIAAFQSGEDVHRRTAA
                     EVFGTAPENVSPEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMINVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
     gene            11475..12170
                     /locus_tag="WM481_001191"
     CDS             11475..12170
                     /locus_tag="WM481_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9080512.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     INGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVNISLLKRINNIVAKDDSLVPV
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPRFNEKLSAFYRTGDDTDISKFVYQNC
                     ISGIDYFGADEDI"
     gene            complement(12162..12272)
                     /locus_tag="WM481_001192"
                     /pseudo
     CDS             complement(12162..12272)
                     /locus_tag="WM481_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12410..16804)
                     /gene="app"
                     /locus_tag="WM481_001193"
     CDS             complement(12410..16804)
                     /gene="app"
                     /locus_tag="WM481_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFA9080513.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQARAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALAGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYADLNKYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPHQNGKYFFND
                     NNNGAGKIDAKHKHYSLPYRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDKGKGELILTSNINQGAGGLYFEGNFTVSPKNNETWQGAGVHISDGSTVTWK
                     VNGVANDRLSKIGKGTLLVQAKGENQGSVSVGDGKVILDQQADDQGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDITTTGNNNNLDSKKEIAYNGWFGEKDATKTNGRLNLNYQPEEADRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWIDRTFKA
                     ENFHIQGGQAVVSRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTSCTEK
                     TITDDKVIASLSKTDIRGNVSLADHAHLNLTGLATLNGNLSAGGDTHYTVTRNATQNG
                     NLSLVGNAQATFNQATLNGNTSASDNASFNLSNNAVQNGSLTLSDNAKANVSHSALNG
                     NVSLADKAVFHFENSRFTGKISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSAADAPRRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSGKLKLAESSEGTYTLAVNNTGNEPVSLEQLTVVEGKDNTPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAGETEAALTAKQAQLAAKQQAE
                     KDNAQSLDALIAAGRDATEKAESVAEPARQAGGENAGIMQAEEEKKRVQADKDTALAK
                     QREAETRPATTAFPRARRARRDLPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQD
                     ELDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFS
                     HNRTGNTFDDGIGNSARLAHGAVFGQYGIGRFDIGISAGAGFSSGSLSDGIRGKIRRR
                     VLHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKA
                     DYSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGF
                     TLSLHAAAAKGPQLEAQHSAGIKLGYRW"
     gene            17242..18588
                     /gene="mnmE"
                     /locus_tag="WM481_001194"
     CDS             17242..18588
                     /gene="mnmE"
                     /locus_tag="WM481_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFA9080514.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPEP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMEMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASGRSAARLALRSLKGDFSRRIHGLV
                     EGLITLRMLVEAALDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRMILDTLPSDLKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLCEAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
BASE COUNT         4287 a         4877 c         5199 g         4547 t
ORIGIN      
        1 aatttagtcc actataaaaa acggcggaaa taaatttttt tccgcctcac ttgaattttc
       61 ccgcacacac cctaattttg ccgacttgta cgggcaggcc gttaggcggc agcccggttt
      121 ccacttcaat ttgtccgaac cgttcgggca gatgattgtt ttcaaaccat ttatcggagc
      181 ataaatatga ccatccgtcc tttacacgac cgcgtcgtcg tcaaacgctt ggaagctgaa
      241 gaaaaaaccg catcgggcat cgtcctgccg ggtgcggccg ccgaaaaacc cgatatgggc
      301 gaagtcatcg ccgtgggcgc gggtaaaatc ggcaaagacg gcgcgcgccg tccgctggat
      361 gtcaaagccg gcgacaaaat cattttcggc aaatacagcg gccaaaccgt aaaagccgac
      421 ggcgaagagc tgttggtaat gcgcgaagaa gatattttcg gcatcgttga aaaataaata
      481 cggacacgat gccgtctgaa acggcaaacc gccttcagac ggcataaacg gttttatcag
      541 acagttttaa tgatttttgg agaattgaaa tggcagcaaa agacgtacaa ttcggcaatg
      601 aagtccgcca aaaaatggta aacggcgcga acattctggc aaacgccgtg cgcgtaacct
      661 tgggccccaa aggccgcaac gtagtggttg accgcgcttt cggcggcccg cacatcacca
      721 aagacggcgt taccgtcgcc aaagaaatcg aactgaaaga caagtttgaa aatatgggcg
      781 cgcaaatggt gaaagaagtc gcgtccaaaa ccaacgacgt agccggcgac ggtacgacta
      841 ccgccaccgt attggcacaa tccatcgttg ccgaaggcat gaaatacgtt accgccggca
      901 tgaacccgac cgatctgaaa cgcggcatcg acaaagccgt tgccgctttg gttgaagagc
      961 tgaaaaacat cgccaaacct tgcgatactt ccaaagaaat cgcccaagtc ggctcgattt
     1021 ccgccaactc cgacgaacaa gtcggcgcga ttatcgccga agcgatggaa aaagtcggca
     1081 aagaaggcgt gattaccgtt gaagacggca aatctttgga aaacgagctg gacgtggttg
     1141 aaggtatgca gttcgaccgc ggctacctgt ccccttactt tatcaacgac gcggaaaaac
     1201 aaatcgccgg tctggacaat ccgtttgttt tgctgttcga caaaaaaatc agcaacatcc
     1261 gcgacctgct gcccgtgttg gaacaagtgg cgaaagccag ccgcccgctg ttgattatcg
     1321 ctgaagacgt agaaggcgaa gccttggcga ctttggtcgt gaacaacatc cgcggcatcc
     1381 tgaaaaccgt tgccgtcaaa gcccccggct tcggcgaccg ccgcaaagcg atgctgcaag
     1441 acatcgccat cctgaccggc ggcgtagtga tttccgaaga agtcggcctg tctttggaaa
     1501 aagcgacttt ggacgacttg ggtcaagcca aacgcatcga aatcggtaaa gaaaacacta
     1561 ccgtcatcga cggcttcggc gacgcagccc aaatcgaagc gcgtgttgcc gaaatccgcc
     1621 aacaaatcga aaccgcgacc agcgattacg acaaagaaaa actgcaagag cgcgttgcca
     1681 aactggcagg aggcgtggca gtgatcaaag tcggcgcggc gaccgaagtc gaaatgaaag
     1741 agaaaaaaga ccgcgtggaa gacgcgctgc acgctacccg cgcagccgtt gaagaaggcg
     1801 tggttgcagg cggcggcgta gccctgttgc gcgcccgcgc cgctttggaa aacctgcaca
     1861 ccggcaatgc cgaccaagac gcaggcgtac aaatcgtatt gcgcgccgtt gagtctccgc
     1921 tgcgccaaat cgttgccaac gcaggcggcg aacccagcgt ggtggtgaac aaagtgttgg
     1981 aaggcaaagg caactacggt tacaacgcag gctccggcga atacggcgac atgatcggaa
     2041 tgggcgtact cgaccctgcc aaagtaaccc gttccgcgct gcaacacgcc gcgtctatcg
     2101 ccggtctgat gctgacgacc gactgcatga ttgccgaaat ccctgaagaa aaaccggctg
     2161 tgcccgatat ggggggaatg ggcggtatgg gcggcatgat gtaagcaatg ccgtctgaaa
     2221 ccttcagaca acaagccgca cggtcaacgc cgtgcggttt ttttgcgaat aagtgcggct
     2281 aaggcgcaaa ccttaaaatt atagcggatt aacaaaaacc ggtacgtcgt tgccccgccc
     2341 cggctcaaag ggaacgattc cctaaggcgc ccaagcaccg ggcgaaccgg ttccgtacca
     2401 ttcgtactgc ctgcggctgg ccgccttgtc ctgatttttg ttaatccgct atatttcccg
     2461 ccatcccaaa aacgaagagc ggcaggaatt tatcggaaaa acagcacctc tccgccgtca
     2521 ttcccgcgaa agcgggaatc taggttcgtc cggtttcggt tatttccgat agattcctgc
     2581 cgcgttgggg gtctggattc ccgtctgcgc gggaatgacg ggactttagg tttctgtttt
     2641 tgtttttctg tttttgcggg aatgacgggg aagttggcgg ttgtttctta tggagtggtt
     2701 tttgtatttg gataaaaaat ttttcgtttt caagccttca ccgcttgcca tcggcgttaa
     2761 aattttttac gataagcaca tagattgtaa acaatcggcc acaagccggt ttgttttttc
     2821 agaagacatt atccctgtca gacgctgttt ctatatatgt ttgcctataa cggcttgttt
     2881 ttaataaata attcaagagg taccaacgtg tctgattcca aaacgaaaga acgcgccaca
     2941 ttcggcacgc gccgcgcgtt tatgattgcc gccatcgggt ccgccgtcgg cttgggcaat
     3001 atttggcgtt tcccctatat tgcttttgaa aacggcggcg gcgcattcat cctgccctat
     3061 ctggtagcgc ttctgacggc gggcatcccg ctgctgctgc tcgattatgc catcggccac
     3121 cgttaccggg gttctgcgcc cttggctttc cgccgcctcg gacgctggtt cgagccggtc
     3181 ggctggtgga acgtgatgac caatatcgtc atctgcatct attacgcagt gattatcggt
     3241 tgggcggcaa gctataccta ttattcggtc aacgccgcct ggggtgcgga tccgcagggt
     3301 tttttcttta aggacttcct gcaaatggcg ggcccggaag ccttgggttt ggattttgtc
     3361 ggcaaagtcg ccggcccttt ggcgggcgtg tgggttttta ccgccgccat tatggctttg
     3421 ggcgtgcaaa agggcgtggc gcgcgcctcg tcgttcttta tgccgctgct tttggtgatg
     3481 tttttgatta tggtcggcat ttcgctgacg ctgccgggtg cggcaaaggg cttggacgca
     3541 ttgtttacgc ccgactggtc gaaactcgcc gattccaagg tctgggtggc ggcatacggg
     3601 cagattttct tttcgctttc catctgcttc ggcattatgg ttacctattc ttcttatttg
     3661 aagaaaaaaa ccgacttggg cggaacgggg ctggtggtcg gttttgccaa cagcagcttc
     3721 gaactgctcg cgggcatcgg cgtgtttgcc gcattgggct ttatggcgca ggcgggcggc
     3781 aaggcggtca acgaggttgc ctccggcggc atcggtttgg cgtttatcgc ctttccgacc
     3841 attatcaacc aggcgccgat gggctggctg atcggcatat tgtttttcgg ttcgctggtg
     3901 ttcgccggcg ttacgtcgat gatttccatc cttgaagtga ttgtggcggc gattcaggac
     3961 aagctgaaca tcgggcgcgt caacgccacg ctgctggtct gcattccgat gggcattgtt
     4021 tccacgctgc tgttcggtac ggcgacgggg ctgcctgttt tggacgtgat ggacaaattc
     4081 gtcaacacct acggcattgt tgccgccggc tttgtttatg ttgccgccat catcatcggc
     4141 ggcaggttgc cggaattacg caggcacctg aacgccttgt cctccatccg cgtcggcggc
     4201 ttgtggacgg cctgcgtcgt gtttaccgtc gtgatgctcg gctatatgct gtatcaggac
     4261 accgccggat tgttggaaaa aaactacggc gattatccgg atggtttcct caatattttc
     4321 ggctggggga tgtcggcggc gttaatcatg ttcgggctgc tgctgtcgct gctgccttgg
     4381 aaacacggtc aggattttaa tgtcaaagac gaacacgaac atgaacaagg aggagaaaaa
     4441 tgagtacttc cgccattgtg atgatggttg ccgccatcgc ggtgatttgg ggcgggctgc
     4501 tgctttccct gttaaggctg ccggaagagt aagcctttag aacgttaaaa atgccgtctg
     4561 aaccgcttca gacggcattt gttttacggc tgccattcgc gttccacctt gcccgacgca
     4621 tccaaagttt gcgctcggac gatatgcccg tcccccggta caggatgcgc gtccgcaata
     4681 tgccctgttc ggtatagcct tgcgccacgc cgttttcaac cggcgtgtgc gaatgcagtt
     4741 tgccgtttgg atggtaaata tcgacaaagc ggtcgaacac gccgtttttg acgatgcttt
     4801 tcaagacgcg cccgtttgag gcgcgggtgc tgaatgcgcc gtccggcgtg cctgaaggcg
     4861 gccggctgta tgggttggcg ggttggatgg gcgcgctttt cggcagggtg cgaagcggcg
     4921 gccggtcaga gggaagggcg gtgcagcccg ccgaaagtag ggcggcggac gaagcggtaa
     4981 gcaggaggat aatcggagcg tgcttcattt caattcctta gatattgcac cccataaaag
     5041 gggcgcgtaa tttttttcaa caattccctt cgtcagtcgg gcgagacggg gatttgttgc
     5101 gtcgtcattt gaaaacaggc tgtgtttgaa tattccgcca agatgtcact ttaaacggac
     5161 aatgccgtct gaaggcttca gacggcattt tgatgttcgg cttataggca ggcgagttcg
     5221 tttgccattt gctgttccaa ggtttcgcgc cggcggatga gtttgtatcc gccgccgtca
     5281 accaacacct ccgccgcacg gttgcgcgtg ttgtaattgc tagccatact ggccccgtat
     5341 gcgcccgcgc tgcggataag cagcaaatcg ccttcttcgc aggcgatggt gcggtctttg
     5401 ccgaggaagt cgccggtttc acaaatcggg ccgacgatgt tggcggtcag aggctcaatg
     5461 tttttggttt caaccgcttc gatgtggtgg taggcatcgt atagggctgg gcgcatcaaa
     5521 tcgttcatcg ccgcatcgac catcacaaag tttttctctt caccgtgttt gacaaattcg
     5581 acgcgcgtca gcaatgcacc tgcgttgccg accaagctgc ggcctggctc aagaatgagt
     5641 ttcagacggc gtgtccccat cagtttttga accgctcggg catacgcacc caaatcgggg
     5701 acgccttcgt ctttgtaaac aatgccgacg ccgccgccta agtccaaatg ttccaaaaca
     5761 atgccttcgg cggcaagagc gtcaaccaaa atcaaaatgc gttcgcaggc ttcgaccagt
     5821 gggcttaagt cggtcagttg cgaaccgatg tggcagtcga tgccgatgat tttcaaattg
     5881 ggctgttgtg cggcatggcg gtaggcttcg agcgcgtcgg cgtaggcgat gccgaatttg
     5941 ttggctttca gacctgtgga gatgtaggga tgggtttttg catcgacatc gggattgacg
     6001 cgcagggaga cgggcgcggt tttgcccaaa cgcgcggcaa ttttctgaat gcggtcgatt
     6061 tcggggatgc tttccatatt gaagcatttt acgccggcat tcagcgcgaa ctcgatttcc
     6121 gcctcgcttt tgcctacgcc ggaaaaaatc gttttcgccg catcgccgcc tgccgccaaa
     6181 acgcgtgcca attcgccgcc cgacacaatg tcaaaaccgc tgcccaaaga agcaaagtgt
     6241 ttgataatgc tcaggttgcc gtttgccttg acggcgtagc agacaagcgg gttcaaagcg
     6301 gcaaaggcgg tttgatagtt ttcaaatgct ccggtcagcg cggattggct gtacacataa
     6361 agcggcgtgc cgaattcttc ggcaaggcgg gggtaggcga cttgttcgca aaacagggtc
     6421 atattttcgt tttcattttt gggttggtgg ggcggattgc ggtttgcctt gaagctgcaa
     6481 accggtttgg attgcgccga aacgcgccgt atcgccttct ttgggcaggt agaggccgcc
     6541 tttgtaaccg caggcggaaa gcaggagggc ggttgccgcc gcaaaaaata cgccgtattt
     6601 catcggtaaa cttccttcat aagcgcgaat gtggcaagat tcggcatctt aaacaaaaaa
     6661 cacgcaaaaa gctatgatga ccgaaagcga gtttatccgc gcgagcgaag cattatttga
     6721 acacatcgaa gaccaaatcg acgaaaacgg ctgggatttc gactgccggt ttgccggaaa
     6781 cgtcctgacc atcgaagccg gagacggcac gcaaatcatc gtcaaccgcc acacgcccaa
     6841 ccaagaattg tggattgccg caaaaagcgg cggctaccat ttcgccgaac aaaacggcaa
     6901 atggctggca acgcgcgaca gccgcgattt ttacgacgtt ttaaacgaag ccctgagcgc
     6961 ggcttcgggc gaagcggttg agattgccga attgtgattc aattttcgat ataaaaagat
     7021 tgttacccaa tggcacaatt acctctatat cggactgccg aaatcggaaa ttttactgtc
     7081 ggcacgccta aagttttaga atcattttcc aaacatatcc cttatggtgt tgtctttgaa
     7141 gatgatggca acacgggcta cttctatgcc gcttcgcaag aggggatttt ggatgccttg
     7201 cacatctaca atgtcgaaga tgtatccgac aaacatatcc ccaatcatgt cttgatttta
     7261 tgggatggcg cctgcaccat agccgcattg tgtatcaacg actacatcca tgccgtctat
     7321 gattttgtcg aacaggcagg atattgccgc aacggcttcc ctgaagcagg cggcgaatgg
     7381 gtaaaagtcg aaaaccgcgt cttggacgat gaattgctgg acaaaatcct atcccgaaaa
     7441 cctacataac ccgcacaaaa ggatacccaa atgcccctgt tagacagttt caaagtcgat
     7501 cacacccgta tgcacgcccc tgccgtacgc gtggcgaaaa ccatgaccac gcccaaaggc
     7561 gacaccatta ctgtattcga cctgcgcttt tgcatcccca ataaagaaat cctgcccgaa
     7621 aaaggcatcc acacgctgga gcatttgttt gccggcttta tgcgcgacca cttgaacggc
     7681 gccggcgtgg aaatcatcga catttccccg atgggctgcc gcaccggttt ttatatgagc
     7741 cttatcggca cgccttccga acagcaggtt gccgatgcgt ggttggcttc gatgcaggat
     7801 gttttgaatg tcaaagacca aagtaaaatc cccgagttga acgaatacca atgcggcact
     7861 tatctgatgc actcgctcgc cgaagcgcaa caaatcgcgc aaaacgtgtt ggcgcgcaaa
     7921 gtggcggtta accgaaacgg cgatttggct ttggatgaaa gcctgctgaa cgcttaatcc
     7981 gccgcaaatg ctgtctgaac aagggtttca gacggcattt gccttttccg ttataatccg
     8041 gggttgtacg ggggcgggtt ttaagccggc atcgtccttc cctatttttc tgtcccttat
     8101 cggtttcaag cgggtttttt atgtccaaca gacctacact cctcctcgtt gacggctctt
     8161 cctatctcta ccgcgcgtat cacgcgatgg ggcaaaacct gactgcgcct gacggtgcgc
     8221 cgacgggtgc gctgtatggc gtattgaata tgttgcgccg tttgcggtcg gaatatccgc
     8281 acgattattg cgcggtggtt tttgatgcga aaggcaaaaa tttccgccat caaatgtttg
     8341 aagaatacaa ggcgacgcgc ccgccgatgc cggacgattt gcgcccgcag gcggaagccc
     8401 tgccggattt ggtgcgcctg acgggctggc cggtattggt gattgggcaa gtggaggcgg
     8461 acgatgtgat cggcacgctg gcgaaacagg gggcggaaca tggtttgcga gtcattgttt
     8521 cgacgggcga taaggatatg gcgcagttgg tggatgagcg cgttacgctg gtgaacacga
     8581 tgagcggaga aacgctggat attgaaggcg tgaaggcaaa attcggcgtt cgccccgacc
     8641 aaatccgcga ttatctcgcg ctgatcggcg acaaggtgga caacgtaccg ggcgtggaaa
     8701 aatgcggccc gaaaacggcg gtgaaatggc tggaagccta cggttcgttg caaggcgtga
     8761 tggaacacgc tccggaaatc aaaggcaagg tcggcgaaaa cctgcaagcc gcgctgcccc
     8821 aactgccgct gtcgtatgat ttggtcacga ttaaaaccga tgtggacttg cacaccgagc
     8881 tttcagacgg catcgaaagc ctgcgccgta ccgcgccgaa atgggcgcag cttgcggttg
     8941 atttcaaacg ctggggcttc cgtacttggc tgaaagaagc ggaatcaaac atgaataccg
     9001 gctcgaccga tgatttgttc ggtagcgaca gcatcggcga gcaggcggct ttgaatgcgg
     9061 aaataccgtc tgaaaaacga gctgaaaaag ccaccgcccc tgaaaaactg gattatcaag
     9121 ccgttaccac cgaagctcag tttgccgcct tgttggacaa actagcgaag gcggacacaa
     9181 tcggcattga tacggaaacc acgtcattag atgcgatgaa tgccgagctg gtcggcatca
     9241 gcattgcgtt ccaagcaggc gaagcggttt acatccccgt aggccacagc ctgactgccg
     9301 cgcccgaaca gcttgatttg caagacgtat taggccgtct gaagccgcat ttggaaaacc
     9361 ccgccctgaa aaaaatcggt caaaacctca aatacgacca acacgttttc gccaactacg
     9421 gcatcgcgtt aaacaacatt gccggcgacg cgatgctcgc ttcctacatc atcgagagcc
     9481 atctcggaca cggcttggac gaattgtccg gacgctggct cggtttagaa accattacct
     9541 acgaatcgct gtgcggcaaa ggcgcgaagc aaatcagttt tgccgatgtc gccatcgggc
     9601 aggcgaccga atacgccgcc caagatgccg atttcgccct gcgcctcgaa gcgcgcctgc
     9661 gcgcgcaaat ggacgacaaa caacttgaaa tgtatgaaaa aatggagctt cccgtcgcgc
     9721 aggtattgtt tgaaatggaa tgcaacggcg tgcaaatcga ccgcgccgaa ctcgcccgcc
     9781 aaagcgcgga actcggcgcg gagctgatga agctcgaaca ggaagcctac gccgctgcag
     9841 gtcaaccgtt taacctcaac tcgcccaaac agctgcaaga aatcctgttc gacaaaatgg
     9901 gcatcccgac aaaagggctg aaaaaaaccg ccaaaggcgg catttccacc aacgaagccg
     9961 tgctcgaaca gctcgcgccc gactaccccc tgcccaaaat catcctgcaa aaccgcagcc
    10021 tggcgaaact caaatccacc tacaccgaca aactgcccga aatgatttcg ccccgggata
    10081 accgcgtgca taccacctat gcccaagccg tcgccattac cggccgcctc gccagcaaca
    10141 accccaacct gcaaaacatc cccattcgca ccgccgaagg ccgccgcgtg cgccgcgcct
    10201 tcactgcacc gccgggcagc gtcatcgttt ccgccgacta ttcccaaatc gagctgcgca
    10261 ttatggcgca cctctccggt gacaaaaccc tcattgccgc atttcaaagc ggcgaagacg
    10321 tacaccgccg caccgccgcc gaagtgttcg gcactgcgcc cgaaaacgtc tcgcccgagc
    10381 aacgccgcta cgccaaaacc atcaacttcg gcttaatcta cggtatgggg caatacggtt
    10441 tggcaaaatc attgggcatc gacaaccttt ccgccaaaaa ctttatcgac cgctacttcg
    10501 cccgttatcc cggcgtcgcc gaatacatgc agcgcaccaa agaacaagcc gccgcccaag
    10561 gctacgtcga aaccctgttc ggcagaaggc tctatttgcc cgacatccgc aacaaaaacg
    10621 ccaacgcccg cgcaggagcc gagcgtgccg ccatcaacgc ccccatgcaa ggcaccgctt
    10681 ccgacctcat caaacgcgcc atgataaacg tgcgaaactg gctttcagac ggcatcggca
    10741 gcaaactggt catgcaggtg catgacgaac tggtgctgga agtcgttgaa accgaactgg
    10801 attttgtcaa agaaaaactg ccgcagatta tggcgaaagt ggacggcgga ttattggatg
    10861 taccgctggt ggctgaggtt ggcgtagggg agaattggga agaggcacat tgagaatgca
    10921 caagtattga actgttacgt tttgatttat caaaatttaa ggaagtaaaa tacccgattc
    10981 gaatgattgg aaatcaatag caagccgtag gtcggatact tgtatccgac aaaagcctgc
    11041 catctcaaat agcagtcgga ttcgagaatc cgacctgcca aaccgggcgc ggacgctccg
    11101 gtcggcagtt agcgcacaaa tcgaacagaa catcacaaaa aagcctgatt cggattttcc
    11161 aatcgggctt ttttgcgccc gttttgtcat cccgtgaaat atccgcataa caaaaataat
    11221 gcaaaatcat gacaaaaccg gcgcgaggtt acacaaacgg atgaaatcaa ccgatattca
    11281 aacacagcca tttttagcgc attttcagcg tatctttatg cggaaaattt cgtgaacagg
    11341 ttttttgcac aggcttccgc cgcgtttcgc gggatgggaa accgtattag aaaacggacg
    11401 cacgcaacat aaaccccgaa agtgatgata agatgatgat ttaacgtact gctttaatta
    11461 tttaaggaat tatcgtgttt cccgacaaat acaagttgag tttggaagaa aatatttttc
    11521 tggcaaagaa agtattggtt gctcaaattc acaacctcag ccgttttgag aattgtcaga
    11581 cgaccttgtt gcagaccgag caaattatca atggcaaaaa tgtagcctcc gcgtcactgg
    11641 aagacatcca aaccatcttg aacctgaaac gtgcctatca atatgtgatt tcgcatattt
    11701 caaacggcga accggtcaat atttcactcc ttaaaagaat caacaacatt gttgccaagg
    11761 acgattcttt ggtacccgtg gatttccgta ccggttcggt cggcgtaacg ctattggacg
    11821 gttcccgtca tgccccgaat ccggtgaagg aaattgaagt ggcccgcgtg ttgcaaaata
    11881 tcggactgca aagcggttcg acgacggaag cagccgttcg tttcatgctc tattgtatgc
    11941 ggcagcaggt tttttgggac ggcaacaaac ggacggcaac cctatttgcc aacggcctga
    12001 tgatggcggg gggctgcggt atcctggaaa tctccgaaat gcagatgccg cgattcaatg
    12061 aaaaactatc cgcattctat cgcaccggcg acgataccga tatttccaag tttgtgtatc
    12121 aaaattgtat atcgggcata gactatttcg gcgcggacga agatatatag tggattaaat
    12181 ttaaaccggt acggcgttgc ctcgccttgc cgtactattt ttactgtctg cggcttcgcc
    12241 gccttgtcct gatttaaatt taatccacta tagatttccc cgactcgccg aacatgggct
    12301 aaaaatcaat ttgacggtta tcagacgatg gagtaggcac aaggtggcag aaaaaggggt
    12361 ttgacagtgc acggcggcat cgtcaaaccc ctttcggcat atccggcggt taccagcggt
    12421 agcctaattt gatgcccgcg ctgtgctgcg cttccaattg cggccccttg gcggcggcag
    12481 cgtggaggga cagcgtgaaa cctttgattt cggcgtttac gccccattcc gcactgcggg
    12541 ttttgccgaa atcctgcgcc aatacggcgg tattgacgcg cgttcggact ttgccggaag
    12601 cggcatcggt ataggacagg ctcaaataag gcgtgatgga aatgtgttgc gccggtttga
    12661 atgaataatc tgccttaatg cccgcgcggt agcggttgaa tgcaaggccc ggggtggcga
    12721 tattgacgtt ttcgtatcgg taatccgctt tttggacgaa atagcgcgtt gcgccgatgt
    12781 gcggttcgat gccgaatccg ccgaaacctg cgcggtatct tgcctgaatg ccgtaatgca
    12841 gcacgcggcg gcggattttg cctctgatgc cgtctgaaag gctgccgcta ctaaaacccg
    12901 cgcccgcgct gatgccgatg tcgaacctgc cgatgccgta ttgcccgaaa acggcaccgt
    12961 gggcaagccg tgccgagttg ccgatgccgt cgtcgaaggt gtttccggtc cggttgtgcg
    13021 aaaacaggat gccgacgcgc ccgctgccga ggtttttctg cataccgatt tggcgcaggt
    13081 cggtttgttg gcggtaggcg cggaaatctt gcgaacggta gtgtttggtg tcccggatgc
    13141 cgcttgtcca aacggcgttg cggcggtctt cggcaaacac gcggtccaat tcgtcctgta
    13201 cggcgaaaac gctgttgagc gtggcggaaa attcactcaa accgctattg gcataacggc
    13261 tgatcaggtc gcgctgcggt tggggttgcg gttgcggcaa atcccggcgg gcgcggcggg
    13321 cgcgggggaa ggcggtggta gccggccggg tttccgcttc gcgctgtttc gccaaggcgg
    13381 tgtctttatc cgcctgcacc cgttttttct cttcctccgc ctgcataatg ccggcatttt
    13441 ccccgcctgc ctgccgggcc ggttcggcaa cgctttctgc cttttcggtg gcatcgcgcc
    13501 cggccgcaat cagcgcgtca aggctttgcg cgttgtcttt ttccgcctgt tgtttggcgg
    13561 caagttgtgc ctgttttgcc gtcaaggcgg cctctgtttc tcccgccttg ccgagtttgt
    13621 cggaaagctc ttgttctttg accggattat gcaggcggaa ctcgccgtct ttgcggataa
    13681 gctgataacg ccatgcgccg gcatcgacgt gttcgttttg cagggtgaaa ttaagatttt
    13741 cggacagcgg tgtgttgtct tttccttcca ctaccgtcaa ttgctcgaga cttacgggtt
    13801 cgttgccggt attgttgaca gccaaggtgt aagtgccttc ggaactttcc gccagcttca
    13861 atttgccgct gcggtagccg aagagttccg acataaagcg gaatgttccc tgaccgttca
    13921 atttgccgtt taccgtcagc gtgttgaaac gggattctgc cgaagttggc ggcgtaacgg
    13981 ataataggga acggcgcgaa cggcggcgcg gcgcatctgc cgcactgccg gtttgcgcgc
    14041 ctgccgcatc gtgtcgatag gcggaattga gtgtaatggt ggcgttgtca aggtttaaat
    14101 tgcctaattc cgtgcccgac ggcagcgtcc attcgctgtc ttttaagtgt aatgccgtat
    14161 ccttgccgcc gctgattttt ccggtaaagc ggctgttttc aaaatggaat actgccttat
    14221 cggctaggga gacattgccg ttgagtgcgg aatggcttac gtttgcctta gcgttgtcgg
    14281 aaagcgtcag actgccgttt tgtacggcgt tgttgcttag attaaatgaa gcattgtccg
    14341 aagccgatgt gttgccgttt aatgtggctt gattaaatgt tgcttgggca ttgcccacga
    14401 ggctgaggtt gccgttttgg gtggcgttgc gcgtaaccgt atagtgcgtg tctccgcctg
    14461 cactaagatt gccgttgagt gtggcaagtc ctgtgagatt taaatgagcg tgatcggcaa
    14521 ggctgacatt gcctctgatg tcggtcttgc tcaatgaagc aatcacttta tcgtcggtaa
    14581 tggttttttc ggtacaactt gtcagacccg tccagtccga acgtgtacag attgtgtggc
    14641 tttgatgcgg tgcgacaccg aaaactgctt gggcgtgatt gcttaaatgc caatcgcctt
    14701 ccactttggc aacattgcgg gaaaccaccg cttgtccgcc ctgaatatgg aagttttccg
    14761 ctttaaatgt gcggtcgatc caatcgttgt cccacacgat ttctccttgt gggatacctt
    14821 ccatttttga ccacccgctt cctaaatgat tgtaggcgtg cggtgtcggt ctgccgctga
    14881 aaaacagttt gccgtttgtt tgcgtgatat tgccgtttaa atttgttccg ccggaaagca
    14941 gtaaagtgcg atccgcttct tccggttggt aattcagatt gagccgcccg ttcgttttgg
    15001 ttgcatcttt ctcgccaaac caaccgttgt aggcaatttc ttttttgcta tccaagttgt
    15061 tgttattgcc ggttgtagta atatctttat tgcctgtaat ggtaacggtg gattctttgt
    15121 cttgattgtg gttgacaatc atcgcccctt catcggtatt ttgaatgcgg tggaacgaaa
    15181 gcgaatgccc gttcaaatcc aaacgtccgc cgcgaaagcc gaaatagagt ttgtcggggt
    15241 tgaactgatt atcggcattc agttgcaccg tccccctgcc gctgaccaag ccgatttcac
    15301 taaaggcttg ttttttgcct tgatcgtccg cctgctgatc taagatgact ttaccgtcgc
    15361 ccacgctgac cgagccttgg ttttcccctt tggcttgaac cagcagcgtg cctttgccga
    15421 ttttggacag gcggtcgttt gccacgccgt ttactttcca agtaacggta ctgccatcac
    15481 tgatatgaac gcccgcgcct tgccacgttt cgttgttttt aggcgagacc gtaaaattac
    15541 cctcaaaata caaaccgccc gcgccttggt tgatgttgct ggtaagtatc aattcacctt
    15601 ttcctttgtc aataaaggaa atattttctc cattattcag tctgggtcga taactgttga
    15661 ccccacctgc agcatgataa acaggttctc ttgctgtctc ggataaagaa acattaaaca
    15721 attgaacggt tcgtgttttt aatctataag gtagagaata gtgtttatgt ttggcatcga
    15781 tttttcctgc gccattatta ttgtcgttaa aaaagtattt cccattttga tgtggttcgt
    15841 agaatactga atgggtatct ccagcaaaga tttcatcata gaaccaatct ttacgaacta
    15901 gctggaagcc attgcttttt cctatatagg ggttgcctgt ttgcaatacc ccattaatta
    15961 accacttttg cttttgggca tcatagataa acattggtga gccactgtcg ccaaatgagc
    16021 ctcctgttgg taaaaaacca tatgggctat gtttaatttt ttcgctacct aagttgactg
    16081 tgccaccacc tgatccattt tgtgcaaagg tattgccacc gacgagccaa gaatatgcgc
    16141 ttgcaatatg atatgaactt tcgcggttat tgggttcgtc ttcatcagac cgccaatatt
    16201 gtctgcctgc tccgattcga acacgatcag ggtatttatt taaatcagcg tatttccacc
    16261 catccatata actggtcatc tcaacaggtt ctgcatctgt gacaaatttg tgcaaacgcg
    16321 gcatatgata atcgccgcca taaggatggc cgttagtccc tgctttataa ttatttcttt
    16381 tcacaatttg gtaagaaaag cggtgctgat cgggattgct tccctccgca ccaaaatcaa
    16441 cattgttata gccgccgtta tgtgccacgc tcacaatata ttgatcgccc gccaatgccg
    16501 ccacgccgtt acgcgatacc acagaaaaat caatcatcgg ggctttcgtc atcgatttgc
    16561 cgaccaactc cccttttttg ttgtaaacct caatatcttt cgccccgact gcaaacttgc
    16621 ctttattttc ggcaaagtcg cgatagtatt ggtagttgat gccgaaataa gtgtgtcccg
    16681 cccgggcttg gggcagaatg ccgaacgaca ggcatatggc taagtaagcg ggcgagaagc
    16741 ggatgcggcc ggttttaggg gctttgcggt gtgtttcggt tgtccgtttg tcggttgttt
    16801 tcattatttt tccttatctg acgggattcg gtttgtttgg gagggcgcgg cttccgcttc
    16861 cgggcggcgc gcgggatgtg gctatatgtg cggttcggcg tattttgccg cttctgttcg
    16921 gagatggtgc agggaagggc gggaaggaaa aaaaggggat ttttatcaac tcgccggagc
    16981 aatcgatggg ttgcacataa aggcatcatt ttgaatacga aatcatatca cactttactt
    17041 tatccccgtt ccttttcggt cggaaccccg ccgttttcgg cggtaaaaac cgaccggagg
    17101 cggaatgcgg ggcagggttt tgtgccgtgc ggtgtcggaa tatcgtttta cacaagccgg
    17161 caatgccatc tgaaagccga accgtcggac ttcagacggc atttgctata atcgcggctg
    17221 ttttgaattt tcgggggttt tatgtcggat aacgttccaa cgattgcggc agtcgcgacc
    17281 gcgcccggac gcggcggcgt gggcgtgata cgcatatcgg ggaaaaacct gttgccgatg
    17341 gcgcaggctt tgtgtgggaa aacgcccgag ccgcgcgtgg caacctatgc tgattttacg
    17401 gacgcggacg gacaggcaat cgatagcggg ctgctactgt tttttgccgc gccggcaagt
    17461 tttacgggcg aagatgtcat cgagcttcag ggacacggcg ggccggtagt gatggagatg
    17521 ctgctgaacc gctgtttgga actgggcgcg cgccttgccg agccgggcga gtttaccaaa
    17581 cgtgcgtttt taaacgacaa actggacttg gcacaggcgg aaggcgtggc ggatttgatt
    17641 gacgcatccg gccgttcggc ggcgcgtctg gcgttgcgct cgctcaaggg cgatttttcg
    17701 cggcggatac acggtttggt cgaaggcttg attaccttgc ggatgctggt cgaagcggcg
    17761 ctggattttc ccgaggaaga catcgatttt ctcgaagcgg cggacgcacg cggcaaactg
    17821 gacggcttgc gccgcgccgt ggatgatgtg cttgccaacg cgcagcaggg cgcgatttta
    17881 cgcgaaggtc tgaatgtcgt attggtcggt gcgccgaatg tcggcaagtc cagcctgttg
    17941 aacgcgctgg cgggcgacga agtggcgatt gttaccgata ttgccggaac gacgcgcgac
    18001 gcggtcaggg aacgtatcct gattgacggc gtgccggtgc atattgtcga tacggcaggt
    18061 ttgcgcgaga cggacgacgt ggtcgaacgc atcggcatcg aacgcagccg caaagccgta
    18121 tccgaagccg atgtcgcgct ggtgttggtc gatccgcgcg agggtttgaa tgaaaagacg
    18181 cggatgattt tggatacgtt gccgtcggat ttgaaacgca ttgaaatcca cagcaaatcc
    18241 gatttgcacg cacacgcggc aggcgggttc ggtacgggcg cggaaaccgt catcgcgctg
    18301 tcggcgaaaa ccggcgacgg cttggacgcg ctgaaacgga cgctgttgtg cgaggccggt
    18361 tggcagggcg aaagcgaagg gctgtttttg gcgcggacgc ggcacgtcaa cgcactcaag
    18421 gcggcgcagg aagaattgtc gctggccgct ttgtgcggca accatcaaat cgagctgttt
    18481 gccgaacact tgcggctggc tcaggtcgcg tgcggcgaaa ttacggggga atttacggcg
    18541 gacgacctgc tcggcgtgat tttttcgagg ttttgcatcg gaaaataagc gggccggagg
    18601 cattgtggcg gtgtccggct gaacattccg ttatcccata aaaacgggaa tccgatccgt
    18661 tcggttttgg ttttttgaat ttcgggcaac gcttaaatct tcattccgcg caggcggaaa
    18721 ttatcggcgc ggtacggcaa cttttttcaa tactgaaagc cccgtcattc ctgtaaaaac
    18781 aaaaaaatca aaaacagaaa actgaaatat cgtcattccc gcgcaggcgg gaatccagcc
    18841 ccgtcggcac ggaaacttat cgggaaaacg gtttctttag attttacgtt ccggattccc
    18901 gctttcgcgg
//
LOCUS       DAWTCI010000035        18560 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWTCI010000035 DAWTCI010000000
VERSION     DAWTCI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18560
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.035"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            324..1376
                     /locus_tag="WM481_001195"
     CDS             324..1376
                     /locus_tag="WM481_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9080515.1"
                     /translation="MRQYLPYSVRWCFFINPGAIILHRQIFLELMLPAAYGGFLTTAL
                     LDRTGFSGNLKPAATLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAWLIWLDR
                     NTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSVLLGTETLK
                     ECRLKDPVFIPNVIYKNIAITLLLHAAAELWLPAQTAGFTALAVGFILLAKLRELHHH
                     ELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMTGGVMMVWLTA
                     GLWHSGFTKLDYPKLCRIAVSILFASAVSRAVLMNVNPIFFITVPEILTAAVFMLYLL
                     TFVPIFRANAFTDDPE"
     gene            complement(1423..3612)
                     /locus_tag="WM481_001196"
     CDS             complement(1423..3612)
                     /locus_tag="WM481_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFA9080516.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSEPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDAARILSVQTVFVEEKPLSQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQANAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYRGQASHSEFVLNTDQQKASDEIQTALGRFRSF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHASAQLPQVDIL
                     NIGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIANDEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSRFGAAPMLMVRLA
                     ERERAQVFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            3752..4537
                     /locus_tag="WM481_001197"
     CDS             3752..4537
                     /locus_tag="WM481_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFA9080517.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAESAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRTQSGYSPMPQLEEIIRKNQQ"
     gene            complement(4636..6570)
                     /locus_tag="WM481_001198"
     CDS             complement(4636..6570)
                     /locus_tag="WM481_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFA9080518.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIQEKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGKIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTRVAEKGLAELLKARHGTEDFFMNNSDSIRQ
                     MVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICIIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(6636..7814)
                     /locus_tag="WM481_001199"
     CDS             complement(6636..7814)
                     /locus_tag="WM481_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFA9080519.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            8040..8429
                     /locus_tag="WM481_001200"
     CDS             8040..8429
                     /locus_tag="WM481_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9080520.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFENYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            complement(8523..12008)
                     /gene="smc"
                     /locus_tag="WM481_001201"
     CDS             complement(8523..12008)
                     /gene="smc"
                     /locus_tag="WM481_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFA9080521.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNNEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHRQNLHQRIERDKQAAQAQLQRIRQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEVQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARSVPNSFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGTHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIAAELENLAPGLSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRTAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIARLEQQTLDWQERRQELALAYETEFQNDEQHIKLDELT
                     ETVHTLDEEYIAVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESAKVLNSSIGSLTRQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAIALLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCKLVKEMSVQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            12401..13069
                     /locus_tag="WM481_001202"
     CDS             12401..13069
                     /locus_tag="WM481_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080522.1"
                     /translation="MGLRQKLRGIIPNLATSIGTSAEANAPGALALGGNSEASKKFSI
                     AEGYLASSDGYGAIAIGSAAKIKQLEKGTINHIVGNDNKGLYVDADGNVTKITVRTES
                     EKDILSRYGQTYGAMALGFRSSSHNLFASSFGAFSTATAIESLAVGDSSQSTGYRSAT
                     FGSHSRALAEESLALGYETRANAYGSVALGAESVANEENTVSVGSDTLKRKIVNVADG
                     TEDL"
     gene            13041..13526
                     /locus_tag="WM481_001203"
     CDS             13041..13526
                     /locus_tag="WM481_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFA9080523.1"
                     /translation="MSPMARKIYDAVNVRQLNRLSKRTNRVGASAAALASLKPAQLGK
                     NDKFAFSLGFGSYKNAQAVAMGAVFKPAENVLLNVAGSFAGPDRAFGAGVSWKFGGKP
                     TPAVAAQNAAHPAKVLQLRQEVAALRARQAETDRKLHKQAEMENELQQLRRALSELKK
                     H"
     gene            13735..14457
                     /gene="lpxH"
                     /locus_tag="WM481_001204"
     CDS             13735..14457
                     /gene="lpxH"
                     /locus_tag="WM481_001204"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFA9080524.1"
                     /translation="MRPSYFISDLHLSEKHPELTELLLRFLRSAAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGRDFCRQAGMTLLPDY
                     SVLDLFGSNTLICHGDTLCTDDKAYLRFRRIVHCRRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPPEEC"
     gene            14726..16312
                     /locus_tag="WM481_001205"
     CDS             14726..16312
                     /locus_tag="WM481_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFA9080525.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYAALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(16478..16681)
                     /locus_tag="WM481_001206"
     CDS             complement(16478..16681)
                     /locus_tag="WM481_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFA9080526.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLGEGESG
                     SVACPYCGTRYRLDGKMPHHYYA"
     gene            16797..17294
                     /locus_tag="WM481_001207"
     CDS             16797..17294
                     /locus_tag="WM481_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFA9080527.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHILSNRAAFA
                     HIGQA"
     gene            17376..>18560
                     /locus_tag="WM481_001208"
     CDS             17376..>18560
                     /locus_tag="WM481_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HFA9080528.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHCLDW
                     DGMIEDLNQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     STQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVK"
BASE COUNT         3943 a         5334 c         4920 g         4363 t
ORIGIN      
        1 cagtatctga caaaccttgc ggttcgtttc cctctcccta accctctccc acaggagagg
       61 ggatcaggtt gctgaaaatc agagggcgaa ggattgggga tcaaatggaa ggtaaaacct
      121 gaaaatgttc gggtttgaca gcccgattcc ctctccccgt gggagagggc tagggagagg
      181 gcaagcaagc cgcaggcttg cctctttggc gaaaaataca accctgtccg cccaataaat
      241 ccatacccga aagcatcttc atacagaatc aagccaagcg atgaataaat tttttaccca
      301 ccctatgcgc ccgtttttcg tcggtgcggc agtacttgcc atactcggtg cgttggtgtt
      361 ttttcatcaa ccccggcgct atcatcctgc accgccaaat tttcttggaa cttatgctgc
      421 cggctgcata cggcggtttt ttgactaccg ctttgttgga ccggacgggt ttttcaggca
      481 acctgaaacc tgccgctact ttgatggcgg tgttgttgct tgttgcggct gttttattgc
      541 cgtttttacc gcaacttgcc gcatttttcg tcgccgccta ttggctggtg ttgctgctgt
      601 tctgcgcctg gctgatttgg ctcgaccgca acaccgacaa cttcgctctg ttgatgttac
      661 ttgccgcatt taccgttttt cagacggcct atgccgtcag cggcgatttg aacttactgc
      721 gcgcgcaagt gcatttgaat atggcggcgg tcatgttcgt atccgtccgc gtcagcgtcc
      781 ttttgggcac ggaaaccctg aaagaatgcc gtctgaaaga ccccgtattc atccccaacg
      841 ttatctataa aaacatcgcc atcaccctgc tgctgcacgc cgccgccgaa ctttggctgc
      901 ccgcgcaaac cgccggtttt actgcgcttg ccgtcggctt catcctgctc gccaagctgc
      961 gcgaactgca ccatcacgaa ctcttacgca aacactacgt ccgcacttat tacctgctcc
     1021 agctctttgc cgccgcaggt tatctgtgga caggcgcggc gaaactgcaa aacctgcccg
     1081 cctccgcgcc cctgcacctg attaccctcg gcggcatgac gggtggcgtg atgatggtgt
     1141 ggctgactgc cggactgtgg cacagcggct ttaccaaact cgactacccg aaactctgcc
     1201 gcatcgccgt ctccatcctt ttcgcctccg ccgtttcgcg cgctgtttta atgaacgtga
     1261 atccgatatt cttcatcacc gttcccgaga ttctgaccgc cgccgtgttc atgctttacc
     1321 tgctgacgtt cgtaccgatt tttcgagcga acgcgtttac agacgatccg gaataaaaat
     1381 gccgtctgaa acggttttca gacggcatcg gattgccaat gatcaagcct cctgcggatc
     1441 gacatccacc gaccatcgga ttttgccgtc gcggttctgc tgcaacacct gcacccacaa
     1501 actcacggcg cggtgcaaat cttgtcggga tgttgattcg aggaaaactt gcgcacgttc
     1561 gcgttcggca aggcgcacca tcagcatcgg ggcggcaccg aaccgggaga cgctttcggg
     1621 caagagcggg gcgagggttt ctttggcggc gttgagaaat tccatcgcat cggcaacacg
     1681 cggcgcatcg gcacggacgg ccgtctgaaa accgaaaggc ggcatggcga acatttgccg
     1741 ttcgttcaat tcgttttcgg caaacacggc gtagtcctgc gctttgacgg cggcgaagac
     1801 agggtgttcg ggcagttggg tctgtatcag caccttgccg ggtttgtcgg cgcgccccgc
     1861 cctgccggac acttgcatca gctcggcaaa cagcctttcc ggcgcacgga aatccgcgct
     1921 gtacaggctg ccgtcggcgt tcaacacgat aacgaggttg agccgcgcga aatcatggcc
     1981 tttggcaagc atctgcgtgc cgaccagaat gtcgatttcg tcgttggcga tgcggcggta
     2041 caaatctgcc cagtcgtttt tgtgcgccgt actgtcccta tcgacgcgga cgacggctgc
     2101 cttgggcagg aaggcgcgca gggtttcttc gacgcgctgc gtaccgtgtc cgacggcggt
     2161 caggtcttgg ttgccgcagt cgggacattt gaacgggatg ggttcgcggt ggtcgcagtg
     2221 gtggcagcgc agttggcggg cgcgttggtg cagcaccatt ttggcggagc agttcgggca
     2281 gccgaaggta tggccgcagt cgccgcaaaa cagcgcaggc gcgaagccgc gccggttaag
     2341 gtacaccagc gacataccgc ccgcttcaaa gttctgtttc aaaagctgca aggcttgcgg
     2401 tgagaagccg ttatcgagtt tcagacgacc tatgttgagg atgtccactt gtggcagttg
     2461 cgcggaagca tgggcgcgtt cggtcagttg cagcaggcgg tacgcgccgc tttgcgcctt
     2521 gtgccaactc tccaagctgg gcgtggcact gcccaacacg acgggacagc cgccctgctt
     2581 cgcccgccac accgccaaat cgcgggcgtg gtagcgcaat tcgttgtctt gtttgaacga
     2641 gccgtcgtgt tcctcatcga ccacaatcag cccgacatca ggcaaaggcg taaacaccgc
     2701 cagccgcgtg ccgatcacca gcttcgcctg ccccaacatc gcgcgcagat aatcctgcgt
     2761 gcgcctgcct gccgccatcc ggctgtgcaa cacggcggtc ggcacgtcgg caaaacggtt
     2821 ttccacccgc tttaaaagct gcggcgtgag gttgatttcg ggcaacagaa acaacacctg
     2881 tcgcccctgc gccaacactt tcgccatcgc atcgaaatac acctcggtct tgccgctgcc
     2941 ggtaatgccg tacagcagaa acgaccggaa gcgtcccaat gccgtctgaa tttcatcgga
     3001 agccttctgc tggtcggtat ttaatacaaa ttcagagtgc gaagcctgcc cgcggtacgg
     3061 ccttaaaaca ggtttcgccg cttccgtcgt ttcaatccag ccctgctccg cccaatcctc
     3121 aatcaatttc gccgcctgcg cgtttgcctg tttcaacgcc gccatcgtca tttcgcccga
     3181 cagcagcgcg tcccacaaag ccgctttttt gttgaaccga gccggcggcg gcgtttgcgc
     3241 cctgcccgct tcgttcaaag cataaaacaa cggcggctgc ggcatttcca ccgcgcgcgt
     3301 ttccttcaaa ccctgcggca atgcggcaaa tacagcctgc ccagtcggat agtggtaata
     3361 acgcgacgta aacgccaaca aatcacgcca gctttgagac agcggctttt cttccacaaa
     3421 gaccgtctga acgctcaaaa tccgcgccgc atccatatcg ggcgcaatat ccgtttccca
     3481 caccatcccg acaacagttt tattgcgaaa aggcacaagc acccgcgttc ccggaggaag
     3541 cggctcggaa tgcgaataag tcaaaaggcc gtctgaaagc ggcacgttta cagcgatgcg
     3601 atggtagatc atacaggcag gcaaatcatt tttttgcgta ttttaacagc cggaagctat
     3661 tttcagacga taaattgagc ctcggcgcaa tgcaggcgca acagaggttt gatacaatgc
     3721 cacttttcca acacgctcaa tcatctttga tatgaaaacc aagttaatca aaatcttgac
     3781 cccctttacc gtcctgccgc tgctggcttg cgggcaaacg cccgtttcca atgccaacgc
     3841 cgaatccgcc gtcaaagccg aatccgccgg caaatccgtt gccgcttctt tgaaagcgcg
     3901 tttggaaaaa acctattccg cccaagattt gaaagtgttg agcgtcagcg aaacaccggt
     3961 caaaggcatt tacgaagtcg tcgtcagcgg caggcagatt atctacaccg atgccgaagg
     4021 cggctatatg ttcgtcggcg aactcatcaa catcgacacg cgcaaaaacc tgaccgaaga
     4081 acgcgccgcc gatttgaaca aaatcgactt cgcctccctg cctttggaca aagccatcaa
     4141 agaagtacgc ggcaacggca agctgaaagt cgccgtcttc tctgaccccg attgtccgtt
     4201 ctgcaaacgc ttggaacatg agtttgaaaa aatgaccgac gtgacggttt acagctttat
     4261 gatgcccatt gccggcctgc acccagatgc cgcgcgcaag gcgcaaatct tatggtgtca
     4321 gcccgaccgt gccaaagcgt ggacggattg gatgcgtaaa ggcaaattcc cggtcggcgg
     4381 cagcatctgc gacaatcccg tcgcggaaac cacttccttg ggcgaacagt tcggcttcaa
     4441 cggcacgccg accctcgtct tccccaacgg gcgcacccaa agcggttaca gcccgatgcc
     4501 ccaactggag gaaatcatcc gcaaaaacca gcagtaaacc cgcaatgcaa aaggcttgga
     4561 accatgttcc aagccttttt caaatgccgt ctgaagcctg tcgcggcagc ttcagacggc
     4621 atctttgttc aaacctcaat cctgcgccaa tgcgtctatc ggattgagtt tggctgcctt
     4681 attggcgggc ataaagccga acgcgatgcc gattccggtc gaacaggcga ccgccccgat
     4741 aacggatgcc gccgaaatgt ccatcgggaa atcggttaca aaatgattga acacgaggct
     4801 gacggcggcg gacaaaccta cgccgaccaa gcctccgatg atgcagatta acaccgcctc
     4861 aatcaaaaac tgctgcaaaa tattgccgcg ccgcgcgccg attgccatcc gtatgccgat
     4921 ttctttggtg cgctcggtaa cggacaccag catgatgttc atcacgccga tgccgccgac
     4981 taccaatgaa atcagggcga tggaggaaat cagcagcttc atcgtaccgg tggtgctttc
     5041 gaccatctgc ctgatgctgt cgctgttgtt cataaagaag tcttccgtgc cgtgccgtgc
     5101 tttgagcagc tcggccagcc ctttttcggc aacccgggta ttggcattgt ctttgatttt
     5161 gacggtgatg gagttggtgt ggctctcgcc tgtgatttgg tgcatcaccg tcgtataggg
     5221 cgaccaaagc atcagcacgt cggaattgcc gaaagcgttt tcgtcttttt tcatcacgcc
     5281 gatgacggtc aaggggcgtt tcctgaacaa aatggtttta cccaacggat ccgagtccgc
     5341 aaagagtttg tctttgacat tttggtcgat gacgacgact tgcgcgtctt ctttcacatc
     5401 gttctcatca aacagccgcc ccgtttccag cttcagcccg cgcacgtcga aatattgttc
     5461 gcccacgccg tacaaagaag cggtcaggtc ggtattgcgg taggtcagcg tgccgccgct
     5521 cgaagtcatg ggcgtggcgg aggcaacgta gctttgtttg gcgatgattt ttgcgtcgtc
     5581 tatggtcagg gttttgattt tgccgctgcg cctgtcgccg aagccgcgcc ccgggaagat
     5641 gctgatggtg ttcgtcccta tcgaactgat gtcttcaagg atttttttct gcgaaccgtt
     5701 gcccaatgcg acgacggaga caaccgaagc gataccgatg atgatgccga gcatcgtcag
     5761 aagcgaacgc attttgtgcg ccaatactgc ttgcaccgac attctgaagg cttcgacaaa
     5821 ctggtcgtaa taaaacgacc acgaggcttt ttcctgaatc ctcccgacat tgcttgcggg
     5881 gatttcggga tttttcgagg tgtcggaaat gatttcgccg tcccggattt cgatgacgcg
     5941 gttggcgttg gcggcaatgc cggggtcgtg cgtgaccata atgacggtat gcccggcttc
     6001 gtgcagctta tggatgattt ccatcacgtt tttgccgctg gcggtatcga gcgcgccggt
     6061 cggctcgtcg gcgaagatga tttctccgcc gttcatcagg gcgcgggcga tggagacgcg
     6121 ctgctgctgt ccgcccgaga gttcgccggg cttgttgccc tctttgcttg ccaaacccaa
     6181 atcctgcaag agtttgtccg cccgcgcgga acgctctttg ccgcccgcgc ccatatagac
     6241 ggctggcagc gcgacgttgt cccttgcggt tagcgagctt aagaggttgt agcgttggaa
     6301 gatgaaaccg aagcgttcgc ggcgcaatgc cgccagctcg tcaggctgca ttttggcagt
     6361 ttcgatgccg tcgattcggt acgaaccgga accggcggta tccaaacagc cgagtatgtt
     6421 catcagcgtg gacttgcccg aaccggactg cccgatgatg gcgacaaaat cgcccttctc
     6481 tatcgacagg ctgatgtctt tcaaaatatg gacgcggttc tcgccgctgc cgaaatagcg
     6541 gttgatgttt ttacattcga tcaagctcat aatattccgt aaatcaataa caaatgccgt
     6601 ctgaaaccgt ttccgtgttc agacggcata ttcgtttatc ggcgcggcgg gccgcctagg
     6661 gcgcgttcgc cgctttcctg ttgctcggcg gcggttattt cggagatgac cactttgtcc
     6721 ccctctttca acccgctttt tacttcggta ttcatactgt ctctcatacc ggtccggatt
     6781 tcgcgttccg ccgccttgcc gtccgcaccc aacacgcgca caaacgcctt gccgccgcga
     6841 tttttcacgg tcagcgacgg aataatcagc acatttttca cgccgtcgat ttcaaccgta
     6901 ttctgcgtcg tcatccccgt ggcgagtttg ccgtccggat tcggcacaaa cgaacgggca
     6961 taatagtaga ccgcattgga agccgtatcc gtactgctgt tgtagccgcc cgacgacatc
     7021 gtggtcagcc cggggtcgac gctgtcgagc ttcgccttaa tcggcgtatc cggttcggac
     7081 aaaatcgtaa acgaaatatc ctgccccgcc ttcaccttgg taatatcgcc ctcggcaatc
     7141 tgcattttgt tcaacatcat atccagattc gccaattgga caatcgtcgg cgtagactgc
     7201 gccgcgttca cagtctgccc ctcttccacg agaatcgcca ccaccgtgcc gtccatcgtt
     7261 gcggtaatgc gcgtgtagcc caattccgac tcggcggtat tgatggaaat tttgctctgt
     7321 ctgattagag ccttcagctc ggcaacattg gctttggcgg cggcaagcgc atcctgcgcg
     7381 ctttccaaat cttctttaga ggtcgcatca tccttccaca acgccgcctg acgcttatat
     7441 ttcttctccg cgctgcccaa tgcaatctgt gccgacacca gcttcgcctg atacgtttcc
     7501 aacttggatt tttccgtatt gagcgtattg gtctgcgagg tcgaattgat ttccgcaatc
     7561 aaatcgccct ttttaacctg ttgcccgagt ttgacataaa gtttcttaat ctgccccgat
     7621 gcctgcgcgc cgaccgatac caggttggac ggcgaaatct cccctgttgc agaaaccgtc
     7681 cggctgatgt cgccgcgcct gaccgtttcc gtaatataag cagcctgcgg ctcgggcttc
     7741 agataagacc atccgcccca aaccgctgcc gccgcgaccg ccgcaacagc cgcccatttc
     7801 atcatttttg ccatgttttc aattctctgt atgcgtcaat cccataaatc ggaagcggat
     7861 tctaccgcaa gtccctgccc catccaaggc ggcaaactgc cgacaataaa aataaccatt
     7921 tgttttaaat ggcttataca atctgctttc ccgaaaattt caatccgttt acatattccg
     7981 ccatttccga tacggcgtta taatgccgtc gaccgacaaa caaatggaaa cacattgcta
     8041 tgaaaaacgt tcaaaaaggc tttacgctgc tcgagctgat gattgccgtc gccatcctcg
     8101 gcatcctgac gcttatcacc tacccttcct ataaaaccta catccggcgc gtccgcctgt
     8161 cggaagtcag gacgaccctg ctgcacaacg cgcagaccat ggaacgttac taccgccaaa
     8221 aagggacgtt tgaaaactac gacaaaaaca aactgaaaca aaacgaatat ttcaagatta
     8281 ccttaagcga agtcagcccc gaccacttca cccttcaggc agcccccgat ccgatgacca
     8341 acgaaggcga aacctgcgtc gttacgctca acgacggcgg caccattgcc gcttccggta
     8401 caaaccaatc ctgccccggc ttcgactgat tggctgttgc agtcggcatt gtgcctaaac
     8461 catctataaa aatgccgtct gaacgctgtt cagacggcat ttttgacatc ccgatacgcc
     8521 tttcaaacgg cttccgccat ttccaacgcc tgcttgatgt ctacggcgac gacgcgcgac
     8581 acgccttttt cctgcatggt tacgccgacc agctgctccg ccatttccat ggtcaggcgg
     8641 ttgtgggaga tgtagaggaa ctgggtttgc accgacattt ctttgaccag cttgcagaaa
     8701 cgcgaggtgt tggcatcgtc cagcggggca tcgacttcgt ccagaaggca gaagggggcg
     8761 gggttgaggc tgaacagggc gaacacgagg ctcatggcgg tgagggcttt ttcgccgccg
     8821 gagaggaggt ggatggtgct gtttttcttg ccgggcggac gcgccatgat ggacacgccg
     8881 gcggtcagga ggtcgtcgcc tatcattttg agggtggctt cgccgccgcc gaacagagtc
     8941 gggaagaagg tttggacttt gccgttgacg gcgtcgaaag tttctttgaa acgcgctttg
     9001 gttttgtcgt cgatttgggc gatggcttct tccaaaagcg cgatggcggc ctgcacgtct
     9061 tcgctttggc tgcggtagta gccgtcgcgt tcgcgcgctt cttcgagttc ttgcagggcg
     9121 gcgaggttga cggcgccgag ggcttcgatt tgccgggtaa ggctgccgat gctgctgttc
     9181 aatactttcg ccgattcttt cgccaacgct tcgagcgcgt ccagatcggc agcgcgttcg
     9241 gtcaggtttt gatggtagcg tttggcgttg atcagggcct cctgctgctg caacagggcg
     9301 gtttgggtgg cggcctgaag ctgcggcagc ttggtttgca gggtttgtac gcgggcgtat
     9361 tgctccctgc cctgctcctg aatctgcgcg agtttttcct gcacggcgat gtattcttcg
     9421 tccagcgtgt gtacggtttc ggtcaactcg tcgagcttga tgtgctgctc gtcgttttgg
     9481 aactcggttt cataggcgag ggcaagctct tgccggcgtt cctgccagtc cagggtttgc
     9541 tgttcgagcc gggcgatttg ctgccggtag ttttgttttt gctggttgag tttgtggacg
     9601 gcgacttcgg caagcccgta ttggcggttg gcttccaaca gggcaagctg cgcctgtttc
     9661 agacggcctt gctgctcttg gcggctgtgc gcggtggttt gctgctggtg ttcgagttcg
     9721 gcggcggctt cctgcaaagt agcgatgtcg tctgaaagcc cgtcggacgt gtgttgcaac
     9781 acggtctgct cttccgccaa ctgtgccagt tcgcgctcga tgtgttcgcg gcggatttgc
     9841 ccttggttgg tgcgcgccag aagttcggcg gtgcgttgct gcgcttgact gtattggcgc
     9901 gtgtgttgct gttgctgctg catcagattt ttgtgctgca cttcggacga gcgcacggca
     9961 gcctccgcct gtttgaacgc ggcttcggcg gcggaaagtc cgggggcgag gttttccagt
    10021 tcggccgcga tgccgtcgag gcgtgctttt tgggcaatca ggctttcctg cgcgggcttg
    10081 gcatagagca ggacgctgac tttatcgacc tgatggcctt cgggtgtgag ccagatttga
    10141 tgcgtgccca aatcgctttg atgcgcgagg gcgtagctca aatcgggcgc gcacaatacg
    10201 ccgtcgagcc agtggtgcaa tgccgtctga aacggcggct gcgcttggat ttggttcagc
    10261 aatgcctgta cgggcaggga ttttttgatg ccgccggaga ggccgtctga aagccatgcc
    10321 gcctgcccct gcggcaaagg tgcgggcggt acgaaactat tcggcacgct acgggcgtgc
    10381 aggcgttcgg caaggatgac ggacagggcg tgctgccact cggcgggcgc ggtgatgtgt
    10441 tgccacagtt gcggcgcggc ggcgtggtcg gttgcctgcc aaaagtcggc ggcttcctgc
    10501 tgttgcgaca ggatttgcga caacgcctgc tgctgcgcct gcaaggtaat gtgttgctgc
    10561 ttcaggcttt ggaagcggtt tgaggccgtc tgaaacgcct cgcgggcggc gtgcaaggct
    10621 tcttcggcgg caatgatttg ttcttcgtaa tgctcttgcc ggctttgcaa caaggcggcg
    10681 gcttcctgag cggcggcggt ttcggcctcg tcgggcaggt tcagggcttg gttttcctgc
    10741 ttcagacggc ctttgcgctc ttcgtgcttg gcaacggttt gttcggcatg ggcaagctgc
    10801 tgctgcttca acgccagttc gcggcggatg cggtttgcct cgtcctgctg ggtttggaag
    10861 gcagcgttga gcgtggcttg gacttcttcc aattcgggca gacgctcttc gtgttcggca
    10921 acctgcatcg cccattccgc caattcggtt tgcttttctt cggcctgcaa ctcgttttct
    10981 tcaagctgca cgcggatttg ctgctgctcc tgccggatgc gttgcaactg cgcctgcgcc
    11041 gcctgcttgt cgcgttcgat gcgttggtgc aggttttgcc ggtggcggat ttgttcttcc
    11101 aaacgggcaa tctgctcgcg caacacgccg cgtttgttgc tcaattcgtg tactgcctgc
    11161 tgctgcgact gttcggcagt ctgcaaggcg tgtacttcgt tatttaacgc ctgaacctgt
    11221 gcggcggttt catcctgctg cgcctgcaaa gattgatgct gcgcggtcgc cttgtcggcg
    11281 gcggcaagcg attgctgcca ttgggcgtaa tcgagcaaat cctgttgttg attcaactgc
    11341 gcggtcaggg atttgtagcg ttcggcggtt tccgcctgtt tttccagctt ttccacctga
    11401 cgcgccaact cgttctgcaa atcgcccaaa cgctgcaaat gctcgcgcgt gtctttcaga
    11461 cggccttccg tctccttgcg gcgttcctta tatttggaca cgcccgccgc ctcctcgata
    11521 taggcgcgca actcctccgg ccgcgcttcg atgatgcgcg aaatcatccc ctgctcaatg
    11581 acggcataac cgcgcgcgcc cacgcccgta cccaagaaca aatcggtaat atcgcggcgg
    11641 cgcacggtct gattgttgat gaaataagtc gattccccct gacgcgtcag ctgccgcttg
    11701 atgctcacct cggcatactg cccccacgcg ccctgcaaac tgtggtcgct gttgtcaaat
    11761 accagctcca ccgaagccct cggcgcagga cggcgcgtcg ccgcaccgtt aaaaatcacg
    11821 tcctgcatac tctcgccgcg aagctgcttc gccgaagcct cgcccaacac ccagcgcacc
    11881 gcgtcaatca cattcgactt gccgcagccg ttcggcccga taaccgcgac aagctgcccc
    11941 ggaacatgaa tcgtggtcgg gtcggtaaaa gatttgaagc cggagagttt gatgtgggtc
    12001 aggcgcataa tggtcggaaa aaataaaaaa gaagcgtatt ttaacggaaa tcccgccgca
    12061 ccaccccata tcttgccggc aaaaccttac cgcatcccgc cccggtgcgg caggattttg
    12121 cacatcccgg ccatccgtat taaaatcttt gcgtttccca cactttcctg acaaatatac
    12181 ggaggaggat tccatgaaaa caaaaacagt caagcaagca agcaagcaag caagcaagca
    12241 agcaagcaag caagcaaaat agtctaaccc gcctttcttt aatcgctcta cttaatttcc
    12301 acttcttttt atcctgttcg atcttttgcg gctgcacctc aagaaataaa ctattcgtta
    12361 tcaggcggta aagtttacgc agataagttc agtatcgcca ttggggttga ggcaaaagct
    12421 cagggggata ataccaaact tagcaacctc aatcggcaca tctgccgaag caaatgcacc
    12481 cggggcattg gctttagggg gaaattctga agcatcgaaa aaatttagta tagcagaagg
    12541 ctatctggca tctagtgatg gctatggtgc aatagcgatt ggttctgccg ccaagataaa
    12601 gcaattggaa aaaggcacaa taaatcatat tgtgggaaac gataataaag gtctttatgt
    12661 cgatgcagac ggcaatgtaa caaagataac cgtaaggact gagtcggaaa aagatattct
    12721 ctcaagatac ggtcaaactt acggtgcgat ggcactaggg tttagatctt cttcacataa
    12781 tctttttgcc agttcatttg gagcgttttc tacagccaca gctattgaaa gcttggcagt
    12841 cggcgacagc agccaatcaa cgggctaccg cagtgctact tttggcagtc acagcagggc
    12901 tttggcagaa gaaagtttgg cattaggtta tgaaactcgg gcaaatgctt atggttccgt
    12961 tgctttaggt gcagaatctg tggcgaatga agaaaatacc gtatcagtgg gttccgatac
    13021 attgaaacgg aaaatcgtca atgtcgccga tggcacggaa gatttatgat gcagtaaatg
    13081 tccgccagtt gaaccgctta agcaaacgta caaaccgcgt cggcgcaagt gctgcggcgt
    13141 tggcttcgtt aaaacctgca caattaggca aaaacgacaa attcgccttt tctttgggct
    13201 ttggaagcta taaaaatgcc caagcggtgg caatgggggc ggtgtttaag cccgctgaaa
    13261 atgtgttgct taatgtggcg ggcagttttg ccggaccgga ccgggctttc ggcgcggggg
    13321 tttcttggaa attcggcggc aaaccgacac ctgcggttgc cgcacaaaac gcggcgcatc
    13381 ctgcaaaagt tttgcaattg cggcaggaag tggcggcgtt gcgcgcccgg caggcagaaa
    13441 ccgaccgcaa actgcacaaa caggccgaaa tggaaaacga gctgcaacag ttgcgccgcg
    13501 cgttgtccga attgaaaaaa cattgaagca ataaacgtaa ggcgggacgg cggctctgcc
    13561 tttttccgat gccgcacccg cgcgatgccg tctgaagcct tcagacggca tttttaacgc
    13621 gcccgaaccc cggttccgcc aatgcccgat gcttggcgcg gcatttgccg tattgccccg
    13681 ccgccgggcg cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgcga
    13741 ccctcctatt tcatttccga cctgcatttg agcgaaaagc accccgaact gaccgaactg
    13801 ctgctgcgtt ttttacgttc tgccgccgcc gggcaggcgc gggcggttta tattttgggc
    13861 gatttgtttg atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg
    13921 cgggaaatca ggaaattgtc cgacaaaggc gttgccgtgt tctttgtcag gggcaaccgc
    13981 gatttcctaa tcggtcggga tttttgccgg caggcgggta tgacgctgct gccggattac
    14041 tcggttttgg acttgttcgg cagcaacacc ctgatttgcc acggcgacac attgtgcacc
    14101 gacgacaagg catacctgcg tttccgccgc atcgtgcatt gcaggcggct gcaaaaactg
    14161 ttcctgatgc tgcccctgaa gtggcgcacg cgccttgccg ccaaaatcag gcgtgtcagc
    14221 aaaatggaaa aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg
    14281 cggcaggttc gcgcctttaa cgcggaaagg ctgatacacg gacacaccca ccgcgagcat
    14341 atccatcacg aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct
    14401 tcaatcctcc gcgtggacgg ggacggcgcg gtattcgtgc cgccggaaga atgctgaaaa
    14461 tgccgtctga agcctttcgg gcggcttttt ctacacgccc ctttcccaca ctccctccgc
    14521 cgcatcatca ccatccttaa cccgcattaa tagccattat cgataaaatg cttgaccgat
    14581 tacataagat tacgtaaagt gtgcaaacgc ataataattg gtcttaccaa tttatctttt
    14641 aaccaaaata tcgggtgtgc gggatttgaa cgtctttcca aacctcgacg cgccttatga
    14701 tgcaaatgcc taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct
    14761 gctgatttgg ctgatggtga aaaaaaacag tatgccttcc tacgtcgcac tgccgattac
    14821 cgcagtgctg atttacgcca tcaaactttt ctacttcggc gatgcgggaa tgctgctcaa
    14881 cgccaccgcc gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc
    14941 gattatgttc aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct
    15001 ggcgaccatc agcccgaacc ccgtagcgca actgatgatt atcggctggg cttttgcctt
    15061 tatgattgaa ggcgcatccg gtttcggcac gcctgccgcg attgccgcgc cgattctgat
    15121 gagcttgggc ttcaatccgt tgaaagtggc gattttcact ttggtgatga attccgtgcc
    15181 ggtatcgttc ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag
    15241 tgccgaagac atcctcgcca tcggcaggca gaccggcgtg atgcacttct tcgcaggttt
    15301 cgtcatccct gtcatcggtt tgggcttcat cgtgccttgg tctgaaatcc gcaaaaactt
    15361 gggcttcgtc gccattgccg tcttctcctg caccattcct tatgccgcat tggcgatggt
    15421 caacgaagaa ttcccgtcgc tcgtcgccgg cgcaatcggc ctgatggtgt ccgtattcgc
    15481 cgccaaccaa ggctggggct tgagcaaaga ccacgccaaa gacccgaatg ccgaaaaagt
    15541 gccgttcgcc caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt
    15601 ggttacccgc atcaaacagc tcggaatcaa aggcattttg accagcaaag aagaatggtt
    15661 cagcttccaa ctgccgtttg atttgtccaa aattaccgtc agcgactccc tgacgattac
    15721 cttcggcaat attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc
    15781 ttggattccg ttcgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa
    15841 agatgcctgg acgatttatg ccgtaacctt caatcaaacc aaaaaaccgc tgcttgccct
    15901 gatgggtgcg ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa
    15961 aatcatcggt aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tttcgccgta
    16021 tctgggcgcg atcggtgcgt tcttctccgg ctccaacacc gtgtccaacc tgaccttcgg
    16081 tccgattcag cagcaaatcg cccttgatac cggcctgtcc gtgaccctga ttctggcgtt
    16141 gcagtccgtc ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg
    16201 taccgtattg gacgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat
    16261 ggcgatttac ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg
    16321 ccgccatcca tagcggcagc acgcggggaa cggaaaccgc cgctcgtccg ggcggcgcgt
    16381 cgggcagaat cccggtgccg gaacagcccg ccctgccaaa caaatgccgt ctgaaaccgg
    16441 aaaaggcttc agacggcatt tttatcgccg tttgcattca ggcgtagtaa tgatgcggca
    16501 tcttgccgtc aaggcggtag cgcgtgccgc aatacgggca ggcaacgctg cccgattctc
    16561 cttcgcccaa aggtaaaaac accctcggat gcccgttcca ctgctcgttg tccggtcccg
    16621 agcaatacag cggcagattt tccggcaaca cggaaatttc ctgcggattc agattgtcca
    16681 tttgattttc ctttgcgtgg ttggcgtgcg cccattttac gccatcggca ggctaaagga
    16741 tattttcggc gcaaagccgc aatccgctat aatccccact tttcagacgg cataccatga
    16801 ctgcgcttac ccttcctgaa gacatccgcc aacaagaacc atccgttctg ctctataccc
    16861 tcgtttccgc ctacctcgaa cacaccgccc aaaccggcga cgaatccctc tcctgcctgt
    16921 ccgacgacca gcacacgctg accgcattct gctacctcga cagccaagtc gaagaaggcg
    16981 gcttcgtaca gcttatcgcc tccggctacg gcgaatacat tttccgcaac ccgcttgccg
    17041 acagcctgcg ccgctggaaa atcaaagccg tgccgaaagt cttggacaaa gccaaagccc
    17101 tctacgaaca acacggcgaa accatcgaaa cgctcgccga cggaggcgca gacatcgaat
    17161 ccctgcgcaa acagttcccc gactttgaag aatgggacgg cgcatactac gaagccgccg
    17221 aacaagacct gcccctgctt gcagaacaca tcctgtcaaa ccgggcggca ttcgcccata
    17281 tcgggcaggc gtgattgcgt ctgtttccag cccgtgtaaa acagcgtaaa atcggcaaac
    17341 ccgaattatc ttccgtcccc atcaaggagc aagctatgtt cttcaagcac atcgaagccg
    17401 cccccgccga tccgattctc ggtttgggcg aagcgttcaa agccgaaacc cgccccgaaa
    17461 aagtcaacct cggcatcggc gtgtacaaag acgcatccgg cgcgacaccc attgtcaaag
    17521 ccgtcaaaga ggctgaaaaa cgcctgttgg aaagcgaaac caccaaaaat tacctgacca
    17581 tcgacggcgt tgccgactac aacgagcaaa cccaaatcct gctgttcggc aaagaccacg
    17641 aaatcatcgc cagccgccgc gccaaaacag cgcaaagcct tggcggtaca ggcgcattgc
    17701 gtattgcggc cgaatttgcc aaacgccagt tgaacgcgca aaccatctgg atttccaatc
    17761 cgacctggcc aaaccacaac gccattgcca aagcggtcgg tatccaagac aaaccttatc
    17821 gctactatga tgccgccaaa cactgcttgg attgggacgg catgattgaa gatttgaacc
    17881 aagcgcaaaa aggcgacatc gtcctgctgc acggctgctg ccacaaccct accggtatcg
    17941 accctacgcc cgaacaatgg gaaactttag caaaactttc tgccgaaaaa ggctggctgc
    18001 cattgtttga ctttgcctac caaggcttcg gcaacggttt ggaagaagat gcctatggct
    18061 tgcgcgtgtt cttgaaacac aatacagaat tgctgattgc cagctcttat tccaaaaact
    18121 tcggcatgta caacgagcgt gtcggtgcat tcactttggt ggccgaagat gaagaaacag
    18181 cagcccgcgc ccacagccaa gtcaaaacca tcatccgtac cttgtattcc aacccggctt
    18241 cacatggtgc gaacaccatt gcactggtgt tgaaaaatga tgatttgaaa gcacaatgga
    18301 ttgccgaact cgatgaaatg cgcggccgca tcaaagccat gcgccaaaaa tttgtcgagt
    18361 tgctcaaggc caaaggttca acccaagact ttgattttat tattgaacaa aacggtatgt
    18421 tctctttcag cggcttgact cccgaacaag ttgaccgttt gaaaaacgag tttgccattt
    18481 atgccgtccg ttccggccgc atcaacgtcg ccggcattac cgacgacaac atcgattatc
    18541 tgtgtgaaag catcgtgaaa
//
LOCUS       DAWTCI010000036        18530 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWTCI010000036 DAWTCI010000000
VERSION     DAWTCI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18530
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.036"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(16..624)
                     /locus_tag="WM481_001209"
     CDS             complement(16..624)
                     /locus_tag="WM481_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFA9080529.1"
                     /translation="MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(688..1281)
                     /locus_tag="WM481_001210"
     CDS             complement(688..1281)
                     /locus_tag="WM481_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFA9080530.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKILVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(1286..1633)
                     /locus_tag="WM481_001211"
     CDS             complement(1286..1633)
                     /locus_tag="WM481_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFA9080531.1"
                     /translation="MRLNHKQGTAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNQRFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIKNITG"
     gene            1680..2555
                     /gene="rsmI"
                     /locus_tag="WM481_001212"
     CDS             1680..2555
                     /gene="rsmI"
                     /locus_tag="WM481_001212"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFA9080532.1"
                     /translation="MFQKHLQKAPDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGRLVSVREHNERQMADKVIGFLSDGLVVAQVSDAGTPAV
                     CDPGAKLARRVREAGFKVVPVVGASAVMAALSVAGVAESDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNAMKILAAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(2590..3120)
                     /locus_tag="WM481_001213"
     CDS             complement(2590..3120)
                     /locus_tag="WM481_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFA9080533.1"
                     /translation="MNTNQPAVYDPLTRALHWLTVAGFIGILTTIVLWTIYEEAEWAG
                     SLFGLHKSFGFLMLTVIALRIVWAVANRAKRPQSDSKAAAAGHGILYLLMLAVPVIGM
                     IRQYGGGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLFAAVVGHVAMVVVH
                     RVQGKDVLYRMTGRVR"
     gene            3399..4553
                     /gene="obgE"
                     /locus_tag="WM481_001214"
     CDS             3399..4553
                     /gene="obgE"
                     /locus_tag="WM481_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFA9080534.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPEPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLAEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            4607..5554
                     /locus_tag="WM481_001215"
     CDS             4607..5554
                     /locus_tag="WM481_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9080535.1"
                     /translation="MYKTIDLFSGIGGIRLGFEKYGCTNVFSSEWDKYARQVYEANFG
                     EKPFGDINGIDPSDIPDHDILLAGFPCQPFSIAGKGLGFEDTRGTLFFNIAEILKTKQ
                     PKAFLLENVKRLTTHDSGRTFRIVLETLKQLGYTVYFKVLNTLDFGLPQKRERIYIVG
                     FSDNIPFYFPEPINQYRPLGELLENDRDVEPSYFLSDTLKQKRLAALKKAPPTPSIWH
                     ENIGGNVSALPYSCALRAGGSYNYLVVNGVRRLTGREMLRLQGFPDDFEINIPYSQVR
                     KVAGNSVSVPVIEAIAENMLASLSGKVEQKGQLDLLEAG"
     gene            5551..6603
                     /locus_tag="WM481_001216"
     CDS             5551..6603
                     /locus_tag="WM481_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeII family restriction endonuclease"
                     /protein_id="HFA9080536.1"
                     /translation="MTLEEQQAKEALDGIIKKSRVHLYKPIQIAEILYHDRCIKQLDF
                     LNLDTYRNQSKRWRDEICRRFLGRISTSSAKFQDNLFEKNAIPPEKLAVLGTLNRQSD
                     GGVESYIYKQFFNRFSQMSEALAYVGNTDRYSFQLSEFLNLFWLEPGLKRSIDKIYEI
                     VVYALFDALVSELGITVSIDFPKENLFLWEEYQDFAEKIITMPKNEHLKLPAKIHRVG
                     VTNAADRGLDMWSNFGLAIQVKHLSLDEELAEDIVSSISADRIVIVCKKAEQSVIVSL
                     LTQIGWKSRIQNIVTEDDLISWYEKALRGQYPIAEALLENIKTEIMREFPAVNEANEF
                     LDFAQNRGYDITVTHF"
     gene            6619..8040
                     /locus_tag="WM481_001217"
     CDS             6619..8040
                     /locus_tag="WM481_001217"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFA9080537.1"
                     /translation="MTAIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELNARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAHL
                     AGCLKALGGIIGLLQRNPIEFLQGGAVSDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDSAGGTTWRRG"
     gene            8211..8990
                     /gene="xth"
                     /locus_tag="WM481_001218"
     CDS             8211..8990
                     /gene="xth"
                     /locus_tag="WM481_001218"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9080538.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LGVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVMHTLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            8977..9321
                     /locus_tag="WM481_001219"
     CDS             8977..9321
                     /locus_tag="WM481_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080539.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAVFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIAAKCLGWKGWRRVVNIFVCLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            9331..9861
                     /locus_tag="WM481_001220"
     CDS             9331..9861
                     /locus_tag="WM481_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9080540.1"
                     /translation="MESFELEDLTLWLIRDADEAEMWIDRWAVSYPVVQMSEASANGS
                     TEAWQSALQTAFERIQGRHIAVVAHGAGAAAFLAWLYRADILTQKKLAGIILVSPRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(9954..11069)
                     /gene="asd"
                     /locus_tag="WM481_001221"
     CDS             complement(9954..11069)
                     /gene="asd"
                     /locus_tag="WM481_001221"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFA9080541.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTSSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIALDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAQVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSGNPTVIDGLCVRIGAMRCHS
                     QAITLKLKKDLPVSEIEAILAGANDWVKVVPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPMRRVLRIVLGSL"
     gene            11244..11825
                     /locus_tag="WM481_001222"
     CDS             11244..11825
                     /locus_tag="WM481_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFA9080542.1"
                     /translation="MEEKNDYTDAVSDNRNGQEIEVGIAGAGDRILAALLNQLFTFLI
                     LLVPFVGLIAFAVKNEGRIGGREEIFGLLLGMTSFWVGLAGILAYTVIQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGENGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(11887..12741)
                     /gene="folD"
                     /locus_tag="WM481_001223"
     CDS             complement(11887..12741)
                     /gene="folD"
                     /locus_tag="WM481_001223"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFA9080543.1"
                     /translation="MSAQLINGKEVSQKHLQAIAEAVAQRQQDNLHTPCLAVVLVGGD
                     PAGAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAILERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAAADILVVGVGIPNFVKG
                     GWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            12964..13041
                     /locus_tag="WM481_001224"
     tRNA            12964..13041
                     /locus_tag="WM481_001224"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12999..13001,aa:Pro,seq:tgg)
     gene            13066..13142
                     /locus_tag="WM481_001225"
     tRNA            13066..13142
                     /locus_tag="WM481_001225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13100..13102,aa:Arg,seq:tct)
     gene            13170..13245
                     /locus_tag="WM481_001226"
     tRNA            13170..13245
                     /locus_tag="WM481_001226"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13203..13205,aa:His,seq:gtg)
     gene            13457..13963
                     /gene="rfaE2"
                     /locus_tag="WM481_001227"
     CDS             13457..13963
                     /gene="rfaE2"
                     /locus_tag="WM481_001227"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HFA9080544.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPVNSLENRAAVAAALESVDLVTWF
                     DEDTPAALIEAVKPEVLVKGGDWVVDKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            13960..15738
                     /locus_tag="WM481_001228"
     CDS             13960..15738
                     /locus_tag="WM481_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFA9080545.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWAFDRPQYELGSLSPVAA
                     LACRRALGCLGLETQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRD
                     HGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGESKKAQVKEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRPAGKRYPFPTTT
                     GNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHA"
     gene            15851..16675
                     /locus_tag="WM481_001229"
     CDS             15851..16675
                     /locus_tag="WM481_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFA9080546.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKAAGGVPENRAVENTPPA
                     TPAAGNAAASVEDTAALLKPGDILSEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLADAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(17181..17429)
                     /locus_tag="WM481_001230"
     CDS             complement(17181..17429)
                     /locus_tag="WM481_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HFA9080547.1"
                     /translation="MAFGGILKKAAKMPSEGFRRHRGPKRANRHSPASRLCREEQGNR
                     PENLAARPDTATNETPGLPGAQAAAQALSTAIEGCFVV"
     gene            complement(17408..18196)
                     /locus_tag="WM481_001231"
     CDS             complement(17408..18196)
                     /locus_tag="WM481_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFA9080548.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     TGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEY"
     gene            complement(18275..18469)
                     /gene="thiS"
                     /locus_tag="WM481_001232"
     CDS             complement(18275..18469)
                     /gene="thiS"
                     /locus_tag="WM481_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFA9080549.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQKPFAVAVNTVFIPKGAY
                     TETVLHENDKIDIVRPVVGG"
BASE COUNT         4201 a         4768 c         5355 g         4206 t
ORIGIN      
        1 gacggcattg ccgagtcagc gttggacgta gttttggtag agggtaatga ctttttgtac
       61 gccgacggtg gtgctgactt tttgggtaat ctgcgcctgt tcttcggggg tgaggatgcc
      121 cataacgtag gttacattgc cgtaggtaat gattttgacg cgcgcctgtg tagcggggct
      181 gatgcccagc agcgtggcgc ggactttgga cgtgttccaa gtgtcgccgg cgatgtcgcc
      241 cgcagtgcgc ggcagggagg cgacggtaat gtagttgtat acgccttcgg cggcctgttc
      301 ggaacgtgca atctgaccga cgaactgttt ttcgccttcg gtggcgactt gtccgagcag
      361 cagcaggtgg cggttgtagc cgacgacgga gatttggggc gtgtagcctt tggtttggtt
      421 gttttggcgc aggtaggaac gggcggtggt ttcgatacgc aacgccataa cgttgtcatc
      481 ggtttgcgcg ccggtggttc ggcggtcgat gacggatttc gcgccgacgg cggccccgcc
      541 gacgactgcg ctgaagcagc cgccgagggc aaggctgagg acggcggcaa tcagggtgcg
      601 gacggtgtgt ggtttgggtt tcatcgggga cttcctttct tgggcgtttc agacggcatt
      661 gctttgcgcc atgccgtctg aaaagggtta cattccttcc agcagtacgg agtcgataca
      721 gtcgcacatg gcgtgtatca gcaggatgtg gttttcctga atgcgggcgg tgcgcggatg
      781 ggggacgttg agcagaacgt cggtgtcttt gagcatggcg gcgattttgc cgccgtcgcg
      841 gccggtcagg gcgatgacgt gcatatcgcg ttcgtgcgcg gctttgacgg cttcgatgac
      901 gttggcggaa ttgccggagg tggaaatgcc gacgaggacg tcgcctgcac gtccgagcgc
      961 gcgcacttgt ttgctgaata cgtggtcgaa accgtagtcg ttgccgatgg cggtcagcgc
     1021 ggaggtgtct gtggtcaggg cgacggcggc gagttccatg cgttctttct caaaacgccc
     1081 ggtcatttcg gcggcgaagt gttgcgcgtc ggcagccgaa ccgccgttgc cgcaggccag
     1141 gattttgccg tcgttcatca ggcattgcag catcagctcg gcagcctgta cggtcggctc
     1201 gaccagtatt ttttcggctt cctgtttggc acggatgctt tcggcaaaat gtgcggcaac
     1261 gcgttcttgt aatgtcgtca tatcgtcaac ctgtaatatt ctttatccac tcgggcgggc
     1321 ggtttccttc gataagtacc gcatcgaggc ggcacggtac gtttgtcagc ctgttctgtt
     1381 gcagataata ctctacactt cgttgcagtt tcaataattt ggacggagaa atgctgtatg
     1441 ccgcgccgcc gaaccgttgg tttttgcggt attttacttc aacaaacaga atcatgccgc
     1501 cgtttttgac aatcaggtcg atttcgccgt aggcgcagtg ccagttgcgg gcaagcagcg
     1561 tgcagccttg ggattggagg aaggcaagtg cggcatcttc ccctgccgta ccctgtttgt
     1621 ggtttaagcg cataatcggt ttaaccttta taatgtctgt ttttcagacg gcatttttta
     1681 tgtttcagaa acacttgcag aaagcccccg acagcgtcgt cggagggaca ttatacgtgg
     1741 ttgccacgcc catcggcaat ttggcagaca ttaccctgcg cgctttggcg gtattgcaaa
     1801 aggcggacat catttgtgcc gaagacacgc gcgttactgc gcagcttttg agcgcgtacg
     1861 gcattcaggg caggttggtc agtgtgcgcg aacacaacga gcggcagatg gcggacaagg
     1921 taatcggttt cctttcagac ggcctggttg tggcgcaggt ttccgatgcg ggtacgccgg
     1981 ccgtgtgcga cccgggcgcg aaactcgccc gccgcgtgcg cgaagcaggg ttcaaagtcg
     2041 ttcccgtcgt gggcgcaagc gcggtaatgg cggcgttgag tgtggccggt gtggcggaat
     2101 ccgattttta tttcaacggt tttgtaccgc cgaaatcggg cgaacgtagg aaattgtttg
     2161 ccaaatgggt gcgggcggca tttcctgtcg tcatgtttga aacgccgcac cgaatcgggg
     2221 caacgcttgc cgatatggcg gaattgttcc ccgaacgccg tctgatgctg gcgcgcgaaa
     2281 tcacgaaaac gtttgaaacg ttcttaagcg gcacggttgg ggaaattcag acggcattgg
     2341 cggcggacgg caaccaatcg cgcggcgaga tggtgttggt gctttatccg gcgcaggatg
     2401 aaaaacacga aggcttgtcc gagtctgcgc aaaatgcgat gaaaatcctt gcggccgagc
     2461 tgccgaccaa gcaggcggcg gagcttgccg ccaagattac aggtgagggc aaaaaggctt
     2521 tgtacgattt ggcactgtcg tggaaaaaca aatgatgccg gatggaccgg caccatagtg
     2581 cgaacggaat caacggacac gccccgtcat gcggtacagc acgtctttgc cttgaacacg
     2641 atggacgacg accatggcga cgtgtccgac gacggcggca aacagcagcc agcccaaatt
     2701 gccgtggaac gtgttgccca agtttgccat ccactcgatt ttttccggcg aaccctgcat
     2761 tacttcaacg ccgaacactt tcaacgggcc gcgtccgcca ccgtattggc ggatcatgcc
     2821 gataacggga acggcaagca tgagcagata cagaatgccg tgtcctgccg ctgccgcctt
     2881 gctgtcgctt tgcggacgct tggcgcggtt ggcaaccgcc cacacgatgc gcaatgcaat
     2941 caccgtcagc atgaggaaac cgaaagattt gtgcaggccg aacaggctgc ccgcccattc
     3001 cgcctcttcg taaattgtcc acaggacaat ggtggtcaga atgccgatga agccggcaac
     3061 ggtcagccag tgcagcgcgc gtgtcaacgg gtcgtaaacg gcaggttggt ttgtgttcat
     3121 aaaaaaatcc ttatcatatg taacggtttg aacggacatt ccgtcgggtc ggaatgtcaa
     3181 aaaggcgcga ttgtaccaaa gaagttggac atattttgtt tgcaggctga agatttgctt
     3241 aaaaattcat taagatgggc agaacaaata gcttctccgc ggtatattga aataccgctt
     3301 ataccttata atgtcggcag aaaatctgtt cagacggcat caaaacatat ggcaatccgc
     3361 ctgaaaccct atttaaaacc aacaaacaag agcatttaat gaaattcatc gacgaagcaa
     3421 aaatcgaagt cgccgcaggc aaaggcggta atggcgcaac cagtttccgc cgcgaaaaat
     3481 tcgtaccccg cggcggcccg gacggtggcg acggcggcaa aggcggcagc gtctgggcag
     3541 aagccgacga aaacaccaac accctcgtcg aataccgctt cgtcaaacgc taccaagcca
     3601 aaaacggcga aaaaggccac ggttccgacc gctacggcgc aggtgcggac gacattgtcc
     3661 tcaaaatgcc cgtcggcacc cttatccgcg acctcgacac cgacgaaatc gttgccgacc
     3721 tcacctacca cggacagcgc gtctgcctcg ccaaaggcgg caaaggcggc ttgggcaaca
     3781 tccacttcaa atcctccgtc aaccgcgccc caaaacaatc cacgcccggc gaagaaggcg
     3841 aaacccgttc cctgcaactc gaactcaaag tcctcgccga tgtcggcttg ttaggcatgc
     3901 ccaacgccgg caaatccacc ctgattaccg ccgtctccgc cgcacgcccc aaaatcgcca
     3961 actacccctt caccaccctg catccgaact tgggcgtggt acgcatcgac gaaaaccaca
     4021 gcttcgtgat ggccgacatc cccggcctga ttgaaggcgc ggcagaaggc gcaggcctcg
     4081 gccaccgctt cctcaaacac ctatcacgca ccggtctgct gttacacgtt gtcgatttgg
     4141 cacccttcga cgaaaccgtc aatcccgccg aagaagcact cgccatcatc aacgaattac
     4201 gcaaatacga cgaagaactt tacggcaaac cgcgctggct ggtgctgaac aaactcgata
     4261 tgctcgacga agaagaagcc cgggcgcgaa cagccgcctt cctcgaagcc gtcggctggg
     4321 actaccccga acctgatgac cgtttccaat tcgacatgga aaccccgcgc ctcttccaaa
     4381 tcagcgcgct gacacatcaa ggcacgcagg agttggtaca ccaaatcaac caatacctgg
     4441 ccgagaaaaa acgtatcgag gctgaaaaag ccgaagcgga gaaagcagcg gcaaatgttg
     4501 aaattatcga acagcagcct aaaacggata ctggcgtgtt taagccggag taaaaggaca
     4561 aggccgtctg aaagttttca ggcggctcat tctgcagaga tgtgctatgt acaaaacaat
     4621 agatttattt tcaggcattg gcggaatacg cctaggcttt gaaaaatacg ggtgcaccaa
     4681 tgtattctca tcggaatggg acaaatatgc ccgacaagtg tatgaagcaa actttggaga
     4741 aaaacctttt ggcgacatta acggcatcga tccttcagat attcccgatc atgatattct
     4801 gcttgcggga tttccgtgcc aacctttcag tatcgcaggt aagggattgg gatttgaaga
     4861 tacgcgcgga acattgtttt tcaatattgc cgaaatcctg aaaaccaaac agccgaaggc
     4921 ttttcttctg gaaaacgtta agcggctgac tacgcacgat tcgggcagga ctttccgtat
     4981 tgttttagaa acccttaaac agcttggtta tacggtttat tttaaggttt taaatacttt
     5041 ggatttcgga ttgccgcaaa aacgcgagag aatttatatt gtgggttttt cagacaacat
     5101 ccctttttac tttcccgaac ccataaacca atatcggcca ttgggcgaac tgctggaaaa
     5161 cgatcgagat gtcgaaccaa gctatttttt atcagatacc ttgaaacaaa aacgtttggc
     5221 agccttaaaa aaagctccgc ccaccccatc aatttggcat gaaaatattg gcgggaatgt
     5281 ttccgcactt ccatattcct gtgccttaag ggccggggga agttacaatt atttggtggt
     5341 aaacggagta agaaggctaa caggaaggga aatgttaaga ttacaaggtt ttcccgatga
     5401 ttttgaaatc aatatcccct actcgcaagt caggaaagtc gccggaaatt ctgtatccgt
     5461 ccctgtaata gaagccatcg cggaaaatat gctcgcttct ctttccggca aggtcgaaca
     5521 aaaagggcaa ttggatttat tggaggcagg atgacgcttg aagaacaaca agccaaagaa
     5581 gcattggacg gcattatcaa aaaatcccgt gtccaccttt ataaacccat tcaaattgca
     5641 gaaattttat atcatgaccg ttgtataaaa cagttggatt ttttaaattt agatacttac
     5701 cgcaaccaat ctaaacgctg gagggatgaa atctgccgtc ggtttttagg gcggatttct
     5761 acttcatctg caaaattcca agataatctg tttgaaaaaa atgcaatacc gcctgaaaaa
     5821 ctagcggtat tgggaacatt aaacagacag tcggacggcg gggtagaatc gtacatttat
     5881 aaacagtttt tcaatcgttt ttcccaaatg agcgaagcat tggcttatgt cggcaataca
     5941 gacagatact ccttccaact gtctgaattt ctaaatttat tttggctcga gccgggattg
     6001 aaaagaagta tagacaagat atatgaaatc gttgtttatg cgttgtttga tgcattggtt
     6061 tcagaattag gcataacggt ttcaattgat tttcctaagg aaaacctgtt tttatgggag
     6121 gaatatcaag attttgccga aaaaatcatc actatgccga aaaatgagca tttaaaactt
     6181 cctgcaaaaa tccatcgtgt aggcgtaacc aatgctgccg atagagggtt ggatatgtgg
     6241 tctaacttcg gattggccat acaggtcaaa catctctctt tagacgaaga gttggcagaa
     6301 gatattgtat cctccattag cgcagataga attgtcattg tctgcaaaaa ggcagaacaa
     6361 tcggtgattg tttcattact gacgcaaata ggctggaaaa gccgaattca aaacatcgta
     6421 actgaggatg atttgataag ttggtacgaa aaagcattga gaggccaata tccgattgca
     6481 gaagcgttgt tggaaaatat taaaactgaa attatgcgtg aatttcctgc cgttaatgaa
     6541 gccaatgaat ttttagattt cgcccaaaat cgcggatatg acattactgt tactcatttc
     6601 taaacttact tgatgaatat gaccgccatc tacaacaccc ttacccgtca aaaagaaccc
     6661 ttcgccccca tcgaccctga aaacgtgcgt atgtacgtct gcggcatgac cgtttacgac
     6721 tattgccact taggccatgc ccgtgtgatg gtggtgttcg acatgattgc ccgctggttg
     6781 cgcgagtgcg gttatccgct cacttatgtg cgcaacatca ccgacatcga cgacaaaatc
     6841 atcgcccgtg cggctgaaaa cggcgaaacc atcggcgaac tgaacgcgcg tttcattcag
     6901 gcgatgcacg aagatgccga tgctttgggc gtgttgcgtc ccgacatcga gccgaaggcg
     6961 acggaaaaca tcccgcaaat gattgccatg attgagaccc tgattcaaaa cggcaaggca
     7021 tatcctgccg caaacggcga cgtttactac gccgtgcgcg agtttgccgc ttacggacaa
     7081 ttatcgggca aatcgttgga cgacctgcgt gcgggcgaac gcgtggaagt ggacggtttc
     7141 aaacgcgatc cgcttgattt tgtattgtgg aaagcggcga aagcaggcga accggcgtgg
     7201 gaaagcccgt ggggcaacgg ccgtccgggt tggcacatcg aatgttccgc gatgagtgaa
     7261 aacctgttcg gcgatacgtt cgacattcac ggcggcggcg cggatttgca gttcccgcac
     7321 catgaaaatg aaatcgccca aagcgtcggt gccagcggtc acacctgcgg tcatgaccac
     7381 gcgcaaaccc accacggtca aagtattgcc agccacgtca aatactggct gcacaacggc
     7441 ttcatccgtg tggacggcga aaaaatgtcc aaatcgctgg gcaacttctt tactatccgc
     7501 gaagtattga aacaatacga ccccgaagtc gtgcgtttct tcatcctgcg cgcccactac
     7561 cgcagcccgt tgaactactc cgacgcgcat ttggacgacg cgaaaggcgc gttgacccgt
     7621 ctgtacacca ccttgaaaaa cacaccggcg gctgagtttg atttgtctga aaacgccaac
     7681 ggctacaccc gccgtttcta cgccgccatg aacgacgatt tcggtacggt cgaagcggtt
     7741 gccgtgttgt ttgagctggc aggcgaagtg aacaaaacca atgacgcaca cctcgccggc
     7801 tgcctgaaag ctttgggcgg catcatcggc ctgctgcaac gcaacccgat tgagttcctc
     7861 caaggcggcg cagtttcaga cggcctctcc aacgaagaaa tcgaagactt gattgcccga
     7921 cgcaaacagg ctcgcgcgga taaaaactgg gcggagtccg accgcatccg cgaccttctg
     7981 aacgaacaca aaatcatttt ggaagacagc gccggcggca cgacttggcg gcgcggttga
     8041 actgtatcgg aaagggcaaa agtaccgcct tttgcccttt tttcgttttc agacggcatt
     8101 tcccgcttga agccccgccg ttttcagcag gcaggaccga atgggaaata ccgggcggcg
     8161 caccgggtgc cgctttgtgt atgggaacat tgtgagaact tatttttatt atgcttaaaa
     8221 tcatttccgc caacgtcaac ggcatccgct ccgcctacaa aaaaggtttt tacgaataca
     8281 tcgccgcatc gggcgcagac attgtctgcg tgcaggaact caaagcgcag gaggctgatt
     8341 tgtctgccga tatgaaaaat ccgcacggga tgcacggcta ttggcattgc gccgagaagc
     8401 gcggttacag cggtgtggcg gtgtacagca aacgcaaacc cgacaatgtg caaatcggca
     8461 tgggcattga ggaattcgac cgcgaagggc ggtttgtgcg ttgcgatttc ggcaggttgg
     8521 gcgtcatttc gctttatttg cccagcggca gcagcgcgga agaacgccag caggtgaaat
     8581 accgtttcct cgatgcgttt taccctatgc tcgaagcgat gaaaaacgaa gggcgcgaca
     8641 tcgtcgtctg cggcgactgg aacatcgccc accaaaacat cgacctgaaa aattggaaag
     8701 gcaatcagaa aaattcgggc ttcctgcccg aagagcgcga gtggataggc aaagtcatgc
     8761 atacgctggg ctggacggac atgtggcgca cgctctatcc cgatgtgccg ggttatacat
     8821 ggtggagcaa ccgcgggcag gcgtatgcga aagatgtcgg gtggcgcatc gattatcaga
     8881 tggttacgcc cgaacttgcc gccaaagccg tgtccgcaca cgtttataaa gatgaaaaat
     8941 tctcggatca cgccccttta gttgtggagt atgactatgc tgccgaataa ggttttgggt
     9001 aaatatgatt ggaatgtgga cggaaaaaca ggaataggcg cggcatgggt tgtcgctgcg
     9061 tttatcctgc ccatattggt gtgggcggtg tttatgttgt cgcggatgca aggctggctt
     9121 gcgccgacca aggcaaaccc gatatgggcg ttggtgtggc tgctgatttg tctgccctgc
     9181 ctgctgattg cggccaaatg tttgggttgg aaaggctggc ggcgggtggt gaatattttt
     9241 gtctgcctga ccgtctgcgc catattgagc gtacccgctt cgctgctcat cgcctttacc
     9301 ctccgggacc tgctcaaata gtcgcggaca atggaaagtt tcgagcttga ggatttgacg
     9361 ctgtggctga tacgcgatgc ggatgaagcg gaaatgtgga tagaccgttg ggcggtcagt
     9421 tatcccgttg tgcagatgtc cgaagcgtcc gcaaacggca gcacggaagc gtggcaaagc
     9481 gcgcttcaga cggcattcga acggatacag ggcaggcata tcgccgttgt cgcacacggc
     9541 gcgggcgcgg ccgcattctt ggcgtggctg taccgcgccg acatcctgac gcagaagaaa
     9601 ctcgccggta tcattctcgt atctccgcgt cccgatattt ttcccgacga tgcggaacac
     9661 actttccaac gcgtccgctg tccttgccgt gccgcattgg tggtgtccga acacggcggc
     9721 gtgccgcacg gttgggcgca aaaacaggcg gatttgtgga acgcccgcct gttggtttcc
     9781 ccgcattcgg gcagtttgaa cggtatgctc ggcggctggc agtggggtat gaagctgatg
     9841 caggaaatgt tgctggcgtg aacggtcgct ttatcggaat ttcggttaac cggggttgca
     9901 aggaaaatac cgtctgaaac aggcttcaga cggcatttcc attcaaacag ggcttacagg
     9961 ctgcccaaca cgatacgcaa cacgcggcgc atcggttcgg ctgcacccca caaaagttgg
    10021 tcgccgacgg tgaacgcgct gatgtattcg ccgcccatgc ccagtttgcg gatgcgtccg
    10081 acagggacgg acagcgtgcc ggtaactttg gcgggcgtca gctcgcggat gctggcttct
    10141 ttttcgttgg ggacgacttt cacccagtcg ttcgcgcctg ccaaaatcgc ttcgatttcg
    10201 gaaacaggca ggtctttttt caacttcaga gtgatggctt ggctgtggca gcgcatcgcg
    10261 ccgatgcgga cgcacaggcc gtcgatgacg gtcggattgc cgctgcgtcc gaggattttg
    10321 ttggtttcca cgccgccctt ccattcttct ttggactggc cgttgcccaa atccacgtca
    10381 atccacggaa tcaggctgcc ggcgagcggt acgccgaagt tggctttcgg atagtcttcg
    10441 ctgcggagga aatcggacac tttgcggtcg atatcgagaa tcgcgctgga aggatcggca
    10501 agctcgtccg ccacttgggc gtgaatcgcg cccatgccgc tgatgagttc gcgcatattt
    10561 ttcgcgcccg cgccggaagc ggcttggtag gtcatgctgg tcgcccattc gaccaaatcg
    10621 ttttggaaca ggccgcccaa agccatcagc atcagggaga cggtacagtt gccgccgatg
    10681 tagtttttca cgccgttttt gagaccgttg tcgatgacgt tgcggttgac ggggtcgagg
    10741 gcgataatcg cgtcgtcttt catgcgcagt gaggacgccg cgtcaatcca gtagccgttc
    10801 cagccgctgt cgcgcagggc ttggaagacg gatttggtgt aatcgccgcc ttggcaggtg
    10861 acgataatgt ccattttcgc cagctcggca acgtcgttcg cgtccaataa tgttttagcc
    10921 gcctgaccga aatcaggtgc cgcgccgccg acgttggaag aggtaaagaa aaacgcttcg
    10981 ggaatgtggg cgaagtcgtt ttcttctttc atacgctgca tcaaaaccga accgaccata
    11041 ccgcgccagc cgacgaaacc tactttcatg tattgctcct tgaggaaaat cgttaataaa
    11101 attatgtatg gcgtaaagaa agcgttttaa cgccgtattg ggaaaatgta aagttcttta
    11161 tcgaattttc tttttgggat tggcgtttgc agcagtaggg cggtagaatt ccatctgttt
    11221 aagaacaatt atcaggaacg gatatggaag aaaaaaacga ctacaccgac gcggtatccg
    11281 acaaccgcaa cgggcaggaa attgaagtcg gaattgccgg cgcaggcgac cggatacttg
    11341 ccgcgctgct gaaccaattg tttacctttt tgattttgtt ggtgccgttt gtcggactga
    11401 ttgccttcgc cgtcaaaaat gaaggcagga ttggcggtcg tgaggaaata ttcgggctgc
    11461 ttttgggcat gacttccttt tgggtcggac tggcgggcat tttggcgtac accgtcatcc
    11521 aaatctacta tatgagcagg gacgggcagt cattgggtaa gaaaatcatg agaatccgcg
    11581 tgttgaaaac cgacggccgc aatccgggtt ttgtcggcac ggttttggta cgcgaaatcg
    11641 cgtggtcggt tttggttgcc attattgccg ccgttatcgg tcttgcagta ggtgaaaacg
    11701 gggaaaacgc catcaacctg ctggcattcc ttgccaactt tgtcctgctc tttatggtca
    11761 aacgcgaccg ccgcacgctt tacgacatat tggcggatac ggttgtcgtc aagctgccca
    11821 aataaacgga cgcggcaaaa tgccgtctga aaggctttca gacggcattt ttatcttcag
    11881 aaccgctcaa gcatcgtgca gcgaagctgc gtgcaaagtg ttttccatta atgtggcaat
    11941 cgtcatcgga cccacgccgc cgggaacggg cgtaatcatc gccgcccgtt cttttgccgt
    12001 ttcaaattcc acgtcgccgc acaggctgcc gtcgtccaaa cggttgatgc ccacatcgat
    12061 aacgaccgcg ccgggtttga tccacccgcc tttgacaaag ttcggaatgc ccacgccgac
    12121 caccaaaata tcggcggcgg caacttcatc ggcaagattt tcggtcgcac tgtggcagac
    12181 cgttaccgtt gcgcgcgcca gcagcaattc caacgcctgc gggcggccga caatattcga
    12241 cgcgccgacc acgaccgctt ttttcccctt cggatcaatg ccgtaagcct ccaaaagcgt
    12301 catcacgccc ttgggcgtac acgggcgcat cagcggcatt ttgaccgcca gcctgccgac
    12361 attgtaagga tggaagccgt ccacgtcctt atccggcgaa atacgttcca agatcgcttg
    12421 gctgtcgagg tgcttcggca gtggaagctg aaccagaata ccgtccactt cggaatcggc
    12481 attcagacgg tcgaccaatg ccagcagttc ttcctgcgat gttgattcgg gcagttcgta
    12541 cgacagcgac ttgatgccgc atttttggca ggcggttttc ttgttgcgga cataaactgc
    12601 gccggcaggg tcgcctccga ccaaaaccac ggccaggcaa ggcgtgtgca gattgtcctg
    12661 ttggcgttgc gccaccgctt cggcaatcgc ctgcaggtgt ttttgcgaaa cttctttacc
    12721 gttgatcagt tgtgccgaca tatccgtcct tttgttaaac aggtagaaaa tgctgaaaat
    12781 tatcgcattt tccgtttgtt tttacagcag aatgccgaaa ataaatttgc cccgaaataa
    12841 aatacggctg tttggacgga ttaaatattt tttcaaatca aaaaactata tttttcatct
    12901 tggtgttttg aaaataaatg ttgacgcatc tttccctgaa ctgtatattc cgcttcttca
    12961 agtcggggcg tagcgcagcc cggttagcgc atctgctttg ggagcagagg gtcgtgagtt
    13021 cgaatcccac cgccccgacc aatcaatcag cacgaatcgg catgagcgcc cgtagctcaa
    13081 ccggatagag caccgacctt ctaagtcggg ggttacaggt tcgattcctg tcgggcgtgc
    13141 caagaatttg attgaaaacc gtttttatgg tggctgtagc tcagttggta gagccccgga
    13201 ttgtgattcc ggttgtcgtg ggttcgagcc ccatcagcca ccccagataa aggcccgtac
    13261 ttttcggtac gggtcttttc ctgtttgcgc gtactgtccg cgccgtcgta ccggtgcggc
    13321 ggtatgccgt ctgaaagccg ggccggtcat acggaaaccg cttcatacgg catttgcgcc
    13381 gccggctttg cctttcgcat atgcttctgt atcatgtggg tttttctgtc ttttatggtt
    13441 tttgtcggag gctttgatgg ttgacgcttg gtctgtccct gattttgaat cgaaaatctg
    13501 tccgcccgag gcgttggcgg cgcgtttggc gttgttgccg cgcccgctgg tgtttaccaa
    13561 cggctgtttc gacatcctcc acagggggca cgttacttat ctggcgcagg cgcgttcggc
    13621 aggggcggcg ttggtgctgg cgttgaatac cgatgcttcg gtgcggcgtt tgggcaaggg
    13681 cggcgaccgc ccggttaatt ctttggagaa ccgtgccgcc gttgccgccg cgttggaaag
    13741 tgtggatttg gttacttggt ttgacgagga tacgccggcg gcgttgattg aggcggtcaa
    13801 gccggaggtg ttggtcaagg gcggcgattg ggtcgtggat aagattgtcg gtgcggcgga
    13861 aacgctggca cgcggcggtc aggtgttttc aattccgttt ctgcatcaga cttcgacaac
    13921 gaagactttg gcaaaaatcc gtgcggcaga gggcggaaaa tgacggtttt gaagccttcg
    13981 cactggcggg tgttggcgga gcttgccgac ggtttgccgc aacacgtatc gcaattggcg
    14041 cgtgaggcgg acatgaagcc gcagcagctc aacggttttt ggcagcagat gccggcgcat
    14101 atacgcgggc tgttgcgcca acacgacggc tattggcggc tggtgcgccc cttggcggtt
    14161 ttcgatgccg aaggtttgcg cgatctgggg gaaaggtcgg gttttcagac ggcattgaag
    14221 cacgagtgcg cgtccagcaa cgacgagata ctggaattgg cgcggattgc gccggacaag
    14281 gcgcacaaaa ccatatgcgt gacccacctg caaagtaagg gcagggggcg gcaggggcgg
    14341 aagtggtcgc accgtttggg cgagtgcctg atgttcagtt tcggctgggc gtttgaccgg
    14401 ccgcagtatg agttgggttc gctgtcgcct gttgcggcac ttgcgtgccg gcgcgctttg
    14461 gggtgtttgg gtttggaaac gcaaatcaag tggccaaacg atttggtcgt cggacgcgac
    14521 aaattgggcg gcattctgat tgaaacagtc agggcgggcg gtaaaacggt tgccgtggtc
    14581 ggtatcggca tcaatttcgt gctgcccaag gaagtggaaa acgccgcttc cgtgcagtcg
    14641 ctgtttcaga cggcatcgcg gcggggcaat gccgatgccg ccgtattgct ggaaacattg
    14701 cttgcggaac tgggcgcggt gttggaacaa tatgcggaag aagggttcgc gccattttta
    14761 aatgagtatg aaacggccaa ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc
    14821 gaaaccgtgt gcgaaggcac ggttaaaggc gtggacggac gaggcgttct gcacttggaa
    14881 acggcagagg gcgaacagac ggtcgtcagc ggcgaaatca gcctgcggcc cgacgacaga
    14941 ccggtttccg tgccgaagcg gcgggattcg gaacgttttt tgctgttgga cggcgggaac
    15001 agccggctca agtgggcgtg ggtggaaaac ggcacgttcg caaccgtcgg cagcgcgccg
    15061 taccgcgatt tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc
    15121 atcgtcggtt gcgccgtgtg cggagaatcc aaaaaggcac aagtgaagga acagctcgcc
    15181 cgaaaaatcg agtggctgcc gtcttccgca caggctttgg gcatacgcaa ccactaccgc
    15241 caccccgaag aacacggttc cgaccgttgg ttcaacgcct tgggcagccg ccgcttcagc
    15301 cgcaacgcct gcgtcgtcgt cagttgcggc acggcggtaa cggttgacgc gctcaccgat
    15361 gacggacatt atctcggcgg aaccatcatg cccggcttcc acctgatgaa agaatcgctc
    15421 gccgtccgaa ccgccaacct caaccgcccc gccggcaaac gttacccttt cccgaccaca
    15481 acgggcaacg ccgtcgcaag cggcatgatg gacgcggttt gcggctcgat aatgatgatg
    15541 cacggccgtt tgaaagaaaa aaacggcgcg ggcaagcctg tcgatgtcat cattaccggc
    15601 ggcggcgcgg cgaaagtcgc cgaagccctg ccgcctgcat ttttggcgga aaataccgtg
    15661 cgcgtggcgg acaacctcgt catccacggg ctgctgaacc tgattgccgc cgaaggcggg
    15721 gaatcggaac acgcttaaaa aatatgcctt ttttcaacac ggggcggcat ccgtccgaat
    15781 ctgtccggat tctgccgccc gacagactgc cgcccgggcg ggcggggttt caaacattga
    15841 ggaaattcta atgaaatggc tcttcaccct tttggcggta ctcaatattg ccgtattcgg
    15901 cggcacggtg ggctataaat tggccgttaa agcggcgggc ggcgtgccgg aaaaccgggc
    15961 cgtcgaaaac acgccccccg caacgcccgc cgccggcaat gcggcggcat ccgtggagga
    16021 tacggcggca ctgctcaaac cgggcgacat cctgagcgaa gaacaggcgg agcagttgcg
    16081 cttgaaaaaa gaagcggaac agaaaaaact gagggagaaa aaacagcgtg aagaaaaagc
    16141 ccgccgcgaa aaactcgccg ccgaaaaggc gcaggcggaa cgtgaaaacg gcgcggcgga
    16201 tgccttatgc gccgcgcagg caagcctcac gatggacgaa gacgactacc accgcatcaa
    16261 agggcttttg ggcaaatggt cgcacgttgc cagcaggagc gtcgaaaaac gcaccgccaa
    16321 agccaaacct gccgacaaaa cctaccgcgt cgtcctgccc gtttccgccg atgccgaaaa
    16381 tcaggcggcg gagctgtctg ccaaaggttt caaccccata ccgtttgacg gcgcattgag
    16441 tttgggtgtc ggcaacagcc gggaaaacgc ccaagccctg caaaaccgac ttgccgatgc
    16501 cggattcggc ggggcgcata ttgtcgaaca ctttgccgaa gccgacaggc aggacgattc
    16561 tttgagcgtg tcgcgtatga cggttttgtt tacgggcgtg aatgccgccg atgcggacga
    16621 aatccgtaaa atcacgtccc tatacggcaa actgaacctc aagtcttgca aataagtgca
    16681 gaccaaaaac gccaaaccca ccgctagggt gggtttggcg tttcggtttt gagtatctga
    16741 ttaaaaaggg aatgtatgtc tcgtatatgc cgactccaag tgtgaaagtg aattgtgagg
    16801 gaatacagtg tttaatggtt cggaacggac ggcttccgag tggttttgat tatacgctaa
    16861 taataataat tatcaataaa aatttaaaaa taaatgcgga ttttttgtaa tgcgatgaaa
    16921 aataaacaaa ttaacctgtc cgccgatgtt tgtgatttgg gtcggcaaac tttcattgcg
    16981 ccggacaaat cggggtcggc aggcatggac gggggcttcg tgcctgtcgg aaagaagcct
    17041 gccgtgtttg aacaaccgtc cgatttggtg cggacagggt tgccattcgg gggtgtttga
    17101 gcgcggattc ggggatgcaa aagcggtgaa accgttgcgg atgaaggctt acggatgccc
    17161 cgaggtattt ttaaatgtat tcaaacgaca aaacaccctt ctattgccgt acttagggct
    17221 tgcgcagcag cctgcgcgcc ggggagtccg ggtgtttcgt tggttgcggt gtcggggcgg
    17281 gcggcaaggt tttccggccg gtttccctgt tcctcacgac aaagccgtga tgccggggaa
    17341 tggcggtttg cccgtttggg gccgcgatgc cgtctgaagc cttcagacgg catttttgcc
    17401 gcctttttca atattccgcc gaatgccaaa acggttgccc gactgtcggc gtgctggctt
    17461 gggctttggt tcgcgcttcg accggcccgg cttcaaatgc cagccgtccg gattcgacgg
    17521 cgagtgcgaa ggcgcgcgcc atgttgacgg ggtcgccgct gcgggaaacg gcggtgttta
    17581 acaatacgcc gtcaaaaccc cattccatca cttgtgccgc ttgggaaggc aaacccaagc
    17641 ccgcgtcgat aatcagcggc gtgtcgggca ggcgttcgcg caggattttg agcgcatagg
    17701 cgtgaaccgc ccccaaaccc gtgccgatgg gagccgccca cggcatcaac gcctgacagc
    17761 ccgcatcgag caggcggcgg caggcaatca ggtcttcggt gcaataaggc aacactttga
    17821 agccgtcttt aatcaggatt tccgccgctt cgacgagttg gaacacgtcc ggctgcaagg
    17881 tgtcgtcgtc gccgatgagt tccaatttta tccaatcggt ttcaaacact tcgcgcgcca
    17941 tttgcgccgt cgttaccgct tcctgcacgc tttggcagcc tgccgtgttc ggcaggacgg
    18001 gaacgccggt ttcttgaagc agcgaccaaa acccctgacc gtgcgcctcg ccgccgcatc
    18061 ccgtgcggcg cagcgagacg gtaatcatcg cgggccgggc ggttcggacg gattgtttga
    18121 ggatttcagg ggtcgggtag gcggctgtgc cgagcagcag ccgcgaaggg aaagtttcgc
    18181 cgtacagggt gagcatgatg gattctttgg tatggttgtc ggacgcgatg ccgtctgaaa
    18241 gccggcggcg tgcctttggg gtttcagacg gcattcagcc gccgacgacg gggcggacga
    18301 tgtcgatttt gtcgttttcg tgtaaaacgg tttccgtata cgcgcctttg gggatgaaaa
    18361 cggtgttgac cgccacggca aagggttttt gcggcgcggt ttgggcgatg aggtcggcaa
    18421 cgcttgtgcc gtgaagttcg gcgggtccgc cgtttaagat gatgttcatg gtttctcgat
    18481 gtctctgtat ttatagcgga ttaacaaaaa ccggtacggc gttgccccgc
//
LOCUS       DAWTCI010000037        18436 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWTCI010000037 DAWTCI010000000
VERSION     DAWTCI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18436
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.037"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(50..1471)
                     /locus_tag="WM481_001233"
     CDS             complement(50..1471)
                     /locus_tag="WM481_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFA9080550.1"
                     /translation="MRHLPLFSLMLFPASVYAADSDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQDRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILITLTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLVFFVFKLL"
     gene            complement(1486..1776)
                     /locus_tag="WM481_001234"
     CDS             complement(1486..1776)
                     /locus_tag="WM481_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HFA9080551.1"
                     /translation="MQIYVKINSFFVFDRLFIKDIYLFVLNKIIYQKEHIFPSTAIRL
                     PAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(1829..2188)
                     /locus_tag="WM481_001235"
     CDS             complement(1829..2188)
                     /locus_tag="WM481_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFA9080552.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2185..2790)
                     /locus_tag="WM481_001236"
     CDS             complement(2185..2790)
                     /locus_tag="WM481_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFA9080553.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2837..3319)
                     /locus_tag="WM481_001237"
     CDS             complement(2837..3319)
                     /locus_tag="WM481_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFA9080554.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3523..3620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3830..4930
                     /gene="gcvT"
                     /locus_tag="WM481_001238"
     CDS             3830..4930
                     /gene="gcvT"
                     /locus_tag="WM481_001238"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFA9080555.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRSGQKQFD"
     gene            5077..5616
                     /locus_tag="WM481_001239"
     CDS             5077..5616
                     /locus_tag="WM481_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080556.1"
                     /translation="MFFDTEGKSHTAIFMKIVGGNGKIAGEVVIDGEYALDIVRMNDD
                     LSIAVQDNKGRSRASDLEALSQAKSFTLIAFSRYAGTVEVDKVSAVTGICADYRSKGL
                     KVAVADNMRPKQEIVVFRASGVMASNKRKINEAYLDYSASSKLPSAQKQELEQDKNNM
                     IAVHYGRLEELIARGICVR"
     gene            5668..6054
                     /gene="gcvH"
                     /locus_tag="WM481_001240"
     CDS             5668..6054
                     /gene="gcvH"
                     /locus_tag="WM481_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFA9080557.1"
                     /translation="MSNNIPTELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQSGVVESVKAASDVYAPIAGEVVAVNDDLPGAPETANSDPYGAGW
                     FFKIKPANPADYDGLLTAEQYAGEVD"
     gene            6207..7454
                     /locus_tag="WM481_001241"
     CDS             6207..7454
                     /locus_tag="WM481_001241"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFA9080558.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYTLDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7626..8816
                     /locus_tag="WM481_001242"
     CDS             7626..8816
                     /locus_tag="WM481_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFA9080559.1"
                     /translation="MIHIISILKSIGISGIAMSCFSIRRMSAFRARITAFFTAFVFLT
                     AALPAYAERLPDFLAKIQPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAV
                     NTRGYSSKPIDTLMALANDGTIAGAKLVDHHEPIMLIGIPQSRVDKFIDKYIGLNFIK
                     NPPTPGVAPGDIISGATVTLMVVNDSIQRSYKVIANQYRLGSDKALQTASASDVREAA
                     PASETRPRRMANPDKQDILSWDELLKQKAVGHLHITLDQINKLFEKGGKAGVADHAEQ
                     GDPDDTFIDLYVALVSQPSIGKSLLGKDGWAHLQKRLKPGQQAVLVAGEGRYSWKGSG
                     YVRGGIFDRIEMIQGENSFRFTDAQHERVVELSAADAPRFKEVSWFTIPEGVAFDGAE
                     PWRL"
     gene            8820..10007
                     /locus_tag="WM481_001243"
     CDS             8820..10007
                     /locus_tag="WM481_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFA9080560.1"
                     /translation="MVQRVLSVNDKAFVTADLDYELPQAYYVDDPKAPPVEISAPVEA
                     VPAAASDTASDGIAEDASAENGVSNQLWKQIWKAKQGQIVVVGIALTILLLVFLFQDW
                     IVRYEKWYDRFRFAFLTFTLFYIGWYAQAQLSVVNTLTLFSAILTEFHWEFFLMDPIV
                     FILWLFTAATMLLWNRGTFCGWLCPFGSLQELTNRIAKKLGVKQITVPHMLHTRLNVI
                     KYLIFFGFFAISLYDLGTAEKFAEVEPFKTAIILKFMCDWWFVAFAVALLIAGLFIER
                     FFCRYLCPLGAGIALPGRFRVFDWLRRYKMCGNPCQICTHECPVQAIAPEGDIHPNEC
                     IQCLHCQVMYHHDTRCPQVVAENKKKQKQAAAKSGELENVSKQPQEQVVRFVKPETAQ
                     SEK"
     gene            10136..12103
                     /gene="nosZ"
                     /locus_tag="WM481_001244"
                     /pseudo
     CDS             10136..12103
                     /gene="nosZ"
                     /locus_tag="WM481_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
     gene            12276..13631
                     /locus_tag="WM481_001245"
                     /pseudo
     CDS             12276..13631
                     /locus_tag="WM481_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            13689..14585
                     /locus_tag="WM481_001246"
     CDS             13689..14585
                     /locus_tag="WM481_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9080561.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVSQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQTAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGPDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIVVMKNGVKVADGSMD
                     ELHVQSGLPLTVNIRLNAPRTLSSRWQPLSDGISYRAQCQAEERMELLGELGSLSDLA
                     YFDIHTPTLDEMYARFLKREDV"
     gene            14582..15412
                     /locus_tag="WM481_001247"
     CDS             14582..15412
                     /locus_tag="WM481_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9080562.1"
                     /translation="MNPVWIITGKEARDSLRNRWVLAAVLLLAALALSLGFLGSSPTG
                     SVKVDPLTVTVVSLSSLSIFLIPLIAMLLSYDALIGEIERGTMALLLSYPIWRNQILA
                     GKFVGHLIILALATTAGYGLAGITLQLANGGFDIAVWKPFALLIAASVILGAAFLSMG
                     YLISAKVKERGTAAGISIGVWLFFVVIFDMALLGILVADSKQVITAPVVETVLLFNPT
                     DIYRLLNLTGYENTAMYAGMAGLSGQIGLTVPVLLTAQVLWVIIPLVLAAGIFRKRRI
                     "
     gene            15409..15903
                     /locus_tag="WM481_001248"
     CDS             15409..15903
                     /locus_tag="WM481_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFA9080563.1"
                     /translation="MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWIDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDA
                     YIFK"
     gene            complement(16037..17698)
                     /locus_tag="WM481_001249"
     CDS             complement(16037..17698)
                     /locus_tag="WM481_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFA9080564.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VAEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGGPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFIYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENFEEVESFDSGKEAGNYQKLFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAVCKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIIE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(17828..18370)
                     /locus_tag="WM481_001250"
     CDS             complement(17828..18370)
                     /locus_tag="WM481_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFA9080565.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEEVL
                     EKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGVDGNTVLLADPSPGHVSMSRAQFLEAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
BASE COUNT         4470 a         4777 c         4959 g         4230 t
ORIGIN      
        1 attatgccgg aatttaaaat gccgtctgaa tgttcagacg gcatagcgtt tacagtagtt
       61 tgaaaacgaa aaagacgagg gtatgtacga tgaagacggg tgtcaggaag gcgaccgacc
      121 acatcatata gccgaagaaa gtcggcatcg gtacgccgcg ctgttcggca atggccttga
      181 ccatgaagtt cggtgcgttg ccgatgtagg tcagtgcgcc catgaatacc gaacccatag
      241 aaaccgccag cagcgaatga aacaggggac ccgtcattaa ggcttgggca tcgccgcccg
      301 ccatattgaa aaacacgaga taagtgggcg cgttatccaa gaatgccgac aatatgccgc
      361 tcatccagaa atacatcgta ttaatcggat gacctgccgt atcgtgaacc agcgatacca
      421 ccccgcccag cgcgcctgcc tcgcctgctt tcagaatgct caggacgggg aagatggtga
      481 tgaagatgcc gaggaagagt ttgcccactt cggcgatggg ttcaaagttg aattcgttgc
      541 ctgcgcggac ttgtttgggc gtgattgcca tagatacggc ggtcaatgta atcaggatga
      601 catcgcggac aaggttttgc agggcgtaac ggctgccgag gatttcaaat cccgggtgtt
      661 cgggtttcca aaggccggac attagaaccg cgccgaccac gcccgaaagc aggaggaagt
      721 tccatttgcc gaagatggcg attttttcgg gtttttcctg ttgtgccggc gtatcttgtg
      781 caatgctttc ctgtttgaag aaacggttgt cgatgaaata gaaggcggtc aacaggacag
      841 cggtgctaat caggacgggg gcgaacatat gtttgaccgt ccacatgaaa tctacgcctt
      901 tgaggaagcc gaggaagagt ggggggtcgc ccaaaggggt caggccgccg ccgatgtttg
      961 caaccaggaa aatgaagaag atgacgatgt gcacgcggcg ggtacggtct tggttggctt
     1021 tcagcagcgg acgaatcatc agcattgccg cgccggtcgt tcccatgata gaggcaagtg
     1081 ccgtaccgac ggcaagcagg gcggtgttga gcttgggtgt gccgttcaag tcgccccaaa
     1141 ccaaaatgcc gcctgaaatg gtgtacaggg caagcagcag caggatgaag gggatatatt
     1201 cttcaacgag tgcgtgtgcg acggtatgga taccggcgga cgcgccaaaa accaaactga
     1261 acgggatgag gaagagcaat gtccaaaagg cggtgatttt gccgtaatgg tgatgccagg
     1321 tatgcgaaaa aaacaaggga cccaatgcga tagacagcaa aatcagggca aagggcaggc
     1381 tccacagcag gtttaggttt gcgccgtccg aatctgcggc gtaaaccgat gcggggaaaa
     1441 gcattagtga aaacaggggt aggtggcgca tcgtgtttcc tcgattcaag cactgccttg
     1501 cgcggcgcgt gggagtgata caggcaccgt gccgcccgga cataggcgga ctgtgctgcc
     1561 tgtttgtttt tgaaaaaagt tttccgattg attgtaaagt aaataatcag gataaaccag
     1621 tttcccaaac cggaaggcgg cgggaaggcg gattgctgtg cttgggaata tatgttcttt
     1681 ttgataaata attttattta aaacaaataa atatatgtct ttaataaata atctgtcgaa
     1741 aacgaaaaat gaatttattt taacatatat ttgcaatgaa acaggtttgc cccccgtttg
     1801 tttgccctta tccctttcag tacggcattc aagattcggg cctgcgccac atccatatgg
     1861 cgacaaggga acaaaaaacc gatgaaaccg ccccgaccca ccagcgttgg ggaaactgcc
     1921 aaaacattat caggcaggat gcggtcatca tactgatggc gaatattttg gctttgcgcg
     1981 gcactgcgcc gttttgttcc cagttatgaa ccatcggacc gaaatagcgg tgccggtgca
     2041 gccagcggtg aaagcgcggg gatgcctttg cccagcaggc ggcggagagc agtacgaacg
     2101 gcgtggtcgg caacagcggc aaaaaaatgc cgatgatacc caacagcagg gaaatgcagc
     2161 cgcaggcaat taaaagataa cgtatcattt tgaaatattt ttcttattgt gcggataagg
     2221 gcaggatgtg ataccgagtt ttgcccagcc ttcatgtccc attttttcca gcagggcgat
     2281 attgcgttcg aatattgccg aagcgtcggg aaaggcttgt gcggctttgg caatgctgtc
     2341 ttcgcggatg aggtgcagcg tcggataggg agaacggttg gtgtagttgc cgatgtcgtc
     2401 gggttcggta ttctgaaagc ggaattgggg atggaacggt gcgatttgga cgatgccttc
     2461 caagccgttt tcgataacgg cggcatcggc aatgtcgagc atatcgttga atacgtcgaa
     2521 atcggggaat agggtcgggt gaaccagcag ggtggtttcc agttcggtgg cgggtgtatt
     2581 gcccagtcgc tgcagttctt cgtccaagtc ttccaaaaaa ctgtcaaggt gtttggcttc
     2641 gctgattgcg atgcggacaa ggtttttaac gtggggggct ttggcaaagg gacacaggtt
     2701 cagaccgatg acggcttttt ccaaccattg tccggtgtgt tcggcaacag catctttatt
     2761 ttcggaagta ttgatattca ttattgtcat gtaaatgtgt ttgcagattg cacgtgcggg
     2821 aaaatcggga agggcactat tccttcagca ggtggttgag cggcagggag gtggtgtgtt
     2881 tgatttcttt taaaacaaag ctcgattgcg catcttgtac gccgtggtgg gacaggagcg
     2941 tatccaaaac aaaatgggaa aacgcgttca tatcggtaaa aaacgcctga agcaggtagt
     3001 cggtttcccc tgtcagggcg aagcagctca agacttccgg ccattttcgg accgatgcgg
     3061 caaagtcttc ccgcgcgtct tttgctttgc ggatggaaac gcggataaat gcctgaagcc
     3121 ccaggttgac ggattccgga gacagcagcg cggcatattg gcggacgatg ccggcatctt
     3181 ccaactgctt cagacggcgc aggcacggag aaggcgaaag tgcgacacgt tcggacagtt
     3241 cgacattggt cagcctgccg ttttcctgga gaacctgtaa gattttaata tcggttttgt
     3301 ctaaagtgag ttggggcata tttgcgttcc gttttaagga attcggattg tctgtccgta
     3361 tgtttgcggc aatccgcaca gatggagacc atattaacat ataaaaagtt ataccgtcat
     3421 ccgggacaaa ttttgttttc ggaaaatcat gtgaaaacag aggcggtcgg tttgcatctc
     3481 ttttaagacg gcttgcccaa accgccgatt caagacataa tcgggaaatg tgcaggagag
     3541 tgttacaccc aactacaatg taaccaccga aggcgcagac acccttaaat cgctcaggta
     3601 tcagggactg cacattgaaa caaacaatct ggagagcggc gttggaataa cgtccaccga
     3661 aggggagaag gccgtctgaa ccaccattca gacaaccgcg caaagcagtg agcagactgg
     3721 tttgccatca tgcggatacg gccgaaaatc tcaggttcaa ggacagatag ggtcatccgc
     3781 gcattttgtg cggacggcat ctgaacaaaa aatccggaga aacttgagaa tgattactct
     3841 gaaaaccacc ccatttcatc aagcccatca agatgcaggc gcgaagccgg tagattttgc
     3901 cggctgggag ctgcccatcc attatggttc gcaaatcgcc gaacacgaag ccgtgcgcac
     3961 cgacgccggt atgtttgacg tatcccatat gcttgttacc gacgtagcag gcgcaaatgc
     4021 caaagccttt ttccgcaaat tgattgccaa cgatgtcgcc aagctcgctt ttgtcggcaa
     4081 agccctttat tccgctttgc tcaacgacaa cggcggcgtg attgacgact taatcgttta
     4141 ccgcaccaac gaagccgaaa cccaataccg catcgtgtcc aacggagcga ctcgcgaaaa
     4201 agacacggcg caattccaca aagtcggaca agagttcggc gttgccttta atccgcgcta
     4261 cgacctcggc atgctcgccg tacaaggccc taaagccatc gaaaaactcc tgaccgtcaa
     4321 acctgaatgg gcggatgtga ttcacggcct caaaccgttc caaggtgcag acttgggcaa
     4381 cgattggttt gtcgcccgca ccggctatac cggcgaagac ggcgtcgaag tgattctgcc
     4441 gggcactgaa gccgtcgcat tcttcaaagc cctgcaagca gcaggcgtac aaccttgcgg
     4501 tctcggcgcg cgcgacaccc tgcgcatgga agccggcatg aacctctacg gcaacgatat
     4561 ggacgacgat accagcccgc tcgaagcagg catgggttgg accgttgatt tgaaagacga
     4621 aagccgcgat tttgtcggca aagctgcctt gttggcattg aaagaaaaag gcgttgccgt
     4681 caaacaggtc ggtttgctgc tcggcaaagg cggcatcctg cgcgcgcata tggaagtgtt
     4741 gaccgacaaa ggcaaaggcg aaaccaccag cggcgtattc tcgcccagcc tgaaacaatc
     4801 catcgccatc gcccgcgtac cgaaagactt tgacggcgat accgctaaag tgctgatgcg
     4861 tggcaaggaa gtggacgtgc gcgtactgaa gctgccgttc gtccgcagcg gtcaaaaaca
     4921 gtttgattaa tatcggttca gacggcattt gtttgaatta agccgtctga aagcaggttt
     4981 taaggaattg ttatttccta tgaaagcaaa ttttggcagt ttattgttgt tattgatgtt
     5041 atcggcttgc gcttcgtcgc cggcgagcgc ggattgatgt ttttcgatac agaaggaaaa
     5101 tcccatactg caatatttat gaaaatcgtt ggcggcaatg gaaagattgc cggcgaggtg
     5161 gtgatagacg gggaatatgc tcttgatatt gtccgcatga atgatgattt gagtattgcc
     5221 gtgcaagata ataagggtag aagccgtgct tccgacctgg aagcattgtc gcaagcgaaa
     5281 tcatttaccc taattgcgtt cagccgttat gcgggcacag ttgaagtgga taaagtatcg
     5341 gctgttacag gcatttgtgc agattaccgc agcaaaggcc tgaaggttgc cgttgcagac
     5401 aatatgaggc ccaaacaaga aatcgttgta ttccgtgcaa gcggcgtaat ggcaagtaat
     5461 aagcgcaaga taaacgaagc atatttggat tattctgcat cctctaaact gccttccgcc
     5521 caaaagcaag aattggaaca agataaaaac aatatgattg ccgtccatta cggacgcttg
     5581 gaagaattaa tcgcacgagg tatctgcgta cgctaagttg tgcagatgcc gtctgaaaat
     5641 aaccccctac caatggagaa tcaaaccatg agcaacaaca tcccaacaga actgaaatac
     5701 gttgccagcc atgaatggct gcgccttgaa gaagacggta ccattaccgt cggtattacc
     5761 catcacgcgc aagagctgtt gggcgacatc gtgtttgtcg agctgcccga agtcggtgcg
     5821 aacctcgccg ccgaagaaca gtccggtgtg gttgagtctg taaaagccgc atccgacgtg
     5881 tatgcgccga ttgcgggcga agtcgttgcc gtcaacgatg acttgccagg cgcgccggaa
     5941 accgccaaca gcgaccctta cggcgcaggc tggttcttca aaatcaaacc tgccaatcct
     6001 gccgattacg acggtctgct gactgccgaa caatacgcgg gcgaagtgga ttaaaccgcc
     6061 cgtttgtccg acggcaaccg tcggacaaac ggaaactgca ccttcagacg gcatttctgc
     6121 ggtcggaggt gcagtttttt tgtccgtgtt ttaaggatgc agttaggcta taataacggt
     6181 ctatatccat cttcaccgat tttttcatgc aacttaccgc tgtcggactc aatcatcaaa
     6241 ccgcaccttt aagcatacgg gaaaagctgg cgtttgccgc cgccgccctg ccagaagccg
     6301 tccgcaatct tgcccgaagc aatgcggcaa cggaggcggt aatcctttct acctgcaacc
     6361 gcaccgagct ttactgcgtc ggcgattcgg aagaaatcat ccgatggctt gccgattacc
     6421 acagtttgcc gattgaagaa atccgtccgt atctgtacac gctggatatg caggaaaccg
     6481 tgcgccacgc cttccgcgtt gcctgcggct tggattcgat ggttttgggc gagccgcaga
     6541 ttttggggca gattaaagat gcggtgcgtg cggctcaaga acaggaaagt atgggggcaa
     6601 aactcaatgc cctgttccaa aaaacctttt ccgttgctaa agaagtccgt accgataccg
     6661 ctgtcggcga aaattcggtt tcgatggctt ccgcgtccgt caagttggcg gaacagattt
     6721 ttcccgacat cggcgatttg aacgtattgt ttatcggcgc aggcgaaatg attgagctgg
     6781 ttgccactta ttttgccgcc aaaaatcccc ggctgatgac ggttgccaac cggacgctgg
     6841 cgcgtgcaca ggagttgtgc gacaagctcg gtgttaacgc ggaaccgtgc ctgctgtccg
     6901 atctgcctgc cattctgcac gattacgacg tggtggtttc ttcaacggcg agccagcttc
     6961 cgatagtcgg caaaggcatg gtcgaacgcg cattgaaaca gcgtcagagt atgccgttgt
     7021 tcatgcttga cttggccgtg ccgcgcgata ttgaagcgga agtcggcgat ttgaacgatg
     7081 cgtatcttta tacggtggac gatatggtca acatcgtcca aagcggcaag gaggcaaggc
     7141 agaaggccgc cgccgccgcc gaaacgctgg tgtccgaaaa ggttgccgaa tttgtcaggc
     7201 agcagcaggg caggcagagc gttccgctga ttaaggcctt gcgggacgag ggcgagaaag
     7261 cgcgcaagca ggtgttggaa aatgcgatga aacagcttgc caaaggcgca acggcggaag
     7321 aggttttgga acggctgtcc gtccaactga ccaacaagct gctgcattcg ccaactcaaa
     7381 ccttgaataa ggcgggggaa gaagataaag atttggttca tgccgtcgcg cagatttatc
     7441 atttggacaa ataacggtgc gccggaaaag gatgccgtct gaaggggttt cagacggcat
     7501 atttttgtgc cgcctgacaa catcgtgaaa tcccacatta tatcgatgta attacaaagt
     7561 attatcctat atgcggattg gttttaagat ttgtaaattt gatttgcatc aaaaatcgcc
     7621 gatagatgat tcatataata tcaatattaa agagtatcgg tatatcgggg atagctatgt
     7681 cctgtttttc aatccggcgt atgtctgcgt ttcgggcgcg gataacggcg ttttttaccg
     7741 cctttgtctt tttgacggcg gcactgcccg cttatgcgga gcgtctgcct gattttctgg
     7801 cgaaaataca gccttcggaa atttttccgg gtgcggatcg ttacggcaag ccggaaggca
     7861 agcctatggt tgcccgcgtt tacaaaggcg atgagcagct cggtttggtt tatatcacga
     7921 ccgatgcggt caatacgcgc ggttattcga gcaaaccgat cgatacgctg atggctttgg
     7981 caaacgacgg cacgatagcc ggggcgaaac tggtcgatca tcacgaaccg attatgctga
     8041 tcggtatccc gcaatcgcgt gtcgataagt tcatcgacaa atatatcggt ctgaatttta
     8101 ttaaaaatcc gccgaccccg ggcgtggcgc cgggcgacat catcagcggt gcgactgtta
     8161 cactgatggt ggttaacgac agcatccagc gttcgtacaa ggtcattgcc aaccaatacc
     8221 gtctgggttc ggacaaggcc cttcagacgg catccgcttc cgatgttcgg gaagccgcgc
     8281 ctgcgtcaga aacccgtccg cgccgtatgg caaatcccga caagcaggat attttgtctt
     8341 gggacgaact tttgaaacaa aaggccgtcg gccatctgca tatcacgctc gatcaaatca
     8401 acaaactgtt tgagaaaggc ggcaaggccg gcgtggccga tcacgccgaa cagggcgatc
     8461 ctgacgatac ctttattgat ttgtatgttg ccttggtcag ccagccttcc atcggtaaaa
     8521 gcctgctggg taaggacggc tgggcgcatc tgcaaaaacg gctgaaaccc gggcagcagg
     8581 cggttttggt tgccggagag ggccgttatt cttggaaagg ttcgggctat gtgcgcggcg
     8641 gtattttcga ccgtatcgag atgattcagg gggagaacag cttccgtttt accgatgccc
     8701 aacacgaacg cgtcgtcgag ctgtctgccg ccgatgcgcc gcgttttaaa gaagtttctt
     8761 ggtttaccat ccctgaaggc gtagcgtttg acggtgcgga gccgtggcgg ctgtaattga
     8821 tggttcagcg cgtattgagt gtgaacgata aagccttcgt taccgccgat ttggattacg
     8881 aactgcccca agcttattac gtcgatgatc ctaaagcgcc gcccgtagaa atcagcgcgc
     8941 ctgtcgaagc agtacctgcc gcagcatccg ataccgcttc agacggcatt gccgaagatg
     9001 cgtcagctga aaacggtgta tccaaccaat tatggaaaca gatttggaaa gcgaaacagg
     9061 ggcagattgt cgtcgtcggc attgcgctga ccatcctgct tttggtattc ctgttccaag
     9121 attggattgt gcgttacgaa aaatggtacg accgcttccg ctttgctttc ctaactttta
     9181 cgctcttcta tatcggctgg tatgcacagg cgcagctttc ggtcgtcaat acgctgacgc
     9241 tgttttccgc catccttacc gaatttcatt gggaattctt cctgatggat ccgattgtgt
     9301 tcatcctgtg gctgttcacg gctgcaacca tgctgctgtg gaacaggggg acgttttgcg
     9361 gttggttgtg tccgttcggc tcattacagg aattgactaa ccgcattgcc aaaaaactgg
     9421 gtgtgaaaca gattaccgtg ccgcatatgc tgcatacccg tttgaacgtc ataaaatacc
     9481 tgattttctt cggttttttt gcaatttcgc tgtatgattt gggtacggcg gaaaaatttg
     9541 ccgaagtcga accgttcaaa actgcgatta tcttgaaatt catgtgcgat tggtggtttg
     9601 tcgcctttgc cgtggcactt ttgattgcgg ggctgtttat cgaacgcttc ttctgccgtt
     9661 atctgtgtcc tttgggtgcg ggcatcgcgc tgccgggcag attccgcgtg ttcgactggc
     9721 tgcgccgcta caaaatgtgc ggcaacccct gccaaatctg tacgcacgaa tgccccgttc
     9781 aggcaatcgc gcccgagggc gacatccatc cgaacgaatg tatccagtgc ctgcactgcc
     9841 aagtgatgta ccaccacgat acgcgctgtc cgcaagttgt ggcggaaaat aagaaaaaac
     9901 aaaaacaggc tgctgccaaa tcaggggaat tggaaaatgt ctctaagcag ccgcaggaac
     9961 aggtcgtccg ttttgtcaaa cccgaaacgg cgcaatctga aaaataagca tccgggggaa
    10021 gcctgaagcc aaagtgtcag gcaggcaggg tttccccgga aggataataa aatttttttc
    10081 ccatcggctt gccgtccgaa aaccggtttg ccgtatttta tccaaggagt gttgtatgtc
    10141 agacgaaaaa ttagaacaaa acggcttgag ccgtcgttcg ttcttaggta cggccgccgc
    10201 ttccggtgca ggtattgccg gtgcgggcct gttgggtttg gcgggttgct ctaaagacgg
    10261 cgaacaggct gccgctaacg cttccggcgc ggctcccgtc gccaaggcgc aaggggaatc
    10321 caaacccggc caactgtctt ccgaagtcgg tccgggcgaa ctcgatcaat attacggttt
    10381 cctttccggc ggccagtccg gcgagatgcg cctgatcggt ctgccttcta tgcgcgaact
    10441 gatgcgtatc cccgtgttca atatggacag cgcgaccggt tggggacgca ccaatgagag
    10501 cttgaaagtc ctcaacggca atattaccga agaaacccgt aaattcttaa aagacggcgg
    10561 cctgcgctgc taccccaacg gcgacttgca ccacccgcac ctgtcgttta ccgaccaaac
    10621 ttatgacggc cgctatgcct atgccaacga caaggcaaac aaccgcgtct gccgcgtgcg
    10681 tttggatgtg atgaaggccg acaaaatcat cgacatccct aatgattcag gtattcacgg
    10741 tctgcgtccg caacgttatc cgaaaaccgg ttatgttttt gccaacggcg aacacattac
    10801 ccctgtcagc ggtgtaggca aactggatga tgcgaaaacc tggaatgcag tgtacaccgc
    10861 catcgacggc gaaaccatgg agatcgcatg gcaagtattg gttgacggta acctggacaa
    10921 cggcgatgcc gactatcaag gcaaatattc ttttgccacc tgctacaact ccgagcgcgc
    10981 gctgaccgta caaggtgcgt cttccaacga gcaggactgg tgcgtcgttt tcgacctgaa
    11041 agccatcgaa gaaggcatca aagcgggcga cttcaaagaa gttaacggtg tgaaaatgct
    11101 ggacggccgc gctgaggcca aatccaaata cacgcgttat atccctgtgc cgaactctcc
    11161 tcacggctgt aacgcaagcc ctgacggtaa atacatcatg cccaacggta aactgcctcc
    11221 aaccgttacc gtattggatg tcagcaaact ggacgatttg ttcgccggca aaatcaaaga
    11281 gcgcgatgtg gtcgtagccg aaccgcaact gggtctcggc ccgttacaca ctgcattcga
    11341 cggtcgcggc aatgcttata cgacattgtt tatcgacagc caaatggtga aatggaatat
    11401 tgacgatgcg atcaaagcct acaaaggcga gaaaatcgat ccgatcaaac aaaaactcga
    11461 cgttcactat caaccgggtc acaaccatac gaccatgggc gaaaccaaag aagccgacgg
    11521 tcaatggctg gtgtccttga acaaattctc caaagaccgc ttcttgaatg ccggtccttt
    11581 gaaacccgag tgcgaccaat tgatcgacat ctccggcgac gaaatgcgtc tggtacacga
    11641 caacccgact tttgccgaac cgcacgactt gtgtttggtt gccgcgtcca aactgaaccc
    11701 gggcaaaact tgggaccgca aagacccgtg gttctagcag gatgctttgg aacaggcgaa
    11761 aaaagacggt gtcgagctgg aaaaagccgc caaagccgta cgcgaaggca acaaagtacg
    11821 cgtgtatatg acggccgtcg cgcctgctta cagtatcccg cagtttgaag tgaaccaagg
    11881 cgacgaagta accgtttacg ttaccaacgt cgaaaccatc gaggacctga cccacggctt
    11941 cactttggaa ggctgcggca tcgctatgga aatcggcccg caagccactt cttccgtaac
    12001 cttcaaagcc gtccgcccgg gtgtgcactg gtactactgc caatggttct gccacgcatt
    12061 gcatatggaa atgtccggtc agatgattgt tcatcccaaa taatgcggcc cgacggctga
    12121 atatggagaa tggcgtctga aggcaacagg tcttcagacg gcatttatag ccaaaccgct
    12181 tgccgtaccg tttcaggcgg tttcgctata aaaacaggca catttgaatg atgctttatt
    12241 ttgttcggcc ataccgaacg aaacagaggg acactatgca cacatccgcg ctccgtatat
    12301 ggctgaaggc cgtgctgata cttgccgccg gcagcatttt tcagacggca tcggcagccg
    12361 tcgtccacgt ttccccgcaa gacaaccttg ccgaaatttt tgcccgcgcc cgggcgggcg
    12421 atacgatcaa actggcttcc ggcgtatatc aaaccaaact ttatatcgac aaaccgatta
    12481 cgattgaagg gcctgccgac cgttccgcaa ccatcgaagg ggacaagagc gggcgtacca
    12541 tagccgtaca cgcgccggac gtaacgctcc gcaacctgac cgttacccgt tccggtatga
    12601 gcctgccagc aatggatgcc ggtatttatc tcgaaaaagc cgccccgcgc cccctggttg
    12661 aacacaacaa tatttttgat aattcgttcg gcgtatacct tcatggttct gccgatgcga
    12721 tggtgcgcga gaataaaatc gtcggcgatg cgaccttgcg cgtgaatgag cgcggcaatg
    12781 gcgttaccgt ttggaacgcg cccggcgcgc aggtcgtcgg caacgacatt tccaaagggc
    12841 gggacggcat tttttccaat accagcacgc acaacaccta taaaaacaac cgcttcagcg
    12901 acctgcgttt cgccgtccac tatatgtaca ccaacgacag cgaagtcagc ggcaatattt
    12961 ccgtgggcaa caatatgggc tatgtgctga tgttttccga acggctcaaa gtgttcgaca
    13021 atatcgccgt cggcagccgc gattagggca tcatgctcaa ctatgtcaac tattccgata
    13081 ttcacgacaa tattatcaac aaagcgggca agtgcgtttt tgcctacaat gccaactacg
    13141 ataaactgtc cgccaatcat tttgaaaact gccaaatcgg catgcacttt accgccgcca
    13201 tcgaaggcac gtccctgcac gacaattcct ttatcaacaa cggaagccag gtcaaatatg
    13261 tcagtacgcg ctttctcgac tggagcgagg gcggacacgg caactactgg agcgacaaca
    13321 gcccgttcga tttgaacggc gacggcttcg gagacagcgc gtaccgtccc gacggcatca
    13381 tcgaccaaat catctggcgc gcgcccgtat cgcgcctctt gatgaacagt cccgcaatca
    13441 gcatcgtcaa atgggcgcag gcgcagtttc ccgccgttct gcccggcggc gtggtggaca
    13501 gcaaaccgct gatgaagcct tatgccccca aaattcaaac ccgttatcag gcgatgaagg
    13561 acgagttgct caaagaagcc gaaacgcggc agtcggaacg gggcagggcg gaaaacggtt
    13621 ctttgaacta gtctgcttca gacggcaccc ggattcaaat gccgtctgaa aacacaaaag
    13681 gaacaaccat gaccacacat catgtcgaat tgaggaaggt aaccaaacgg ttcggggcgc
    13741 aaaaagccgt cagccaagtc gatttggttt tgaaggcagg agaaagcgtc gggcttgccg
    13801 gacacaacgg cgcgggcaag tccaccatca tgaagctgat actcgggctg attaccccga
    13861 ccgaaggcga agtgatgctt ttgggcgaac gtaccggcag caaagcgggg gcgcggcttc
    13921 gcagccaaat cggctacctg cccgaaaccg ttgcgctgca cccttcgctg accggcatcg
    13981 aaacgctgga tttttatgcc aaacttaaaa aacagccgct cacgcagaac cgggggctgc
    14041 ttgagcgcgt cggcatttcc cagaccgcac accgccgcgt cggcacttat tctaaaggga
    14101 tgcgccaacg ccttgccttg gcacaagccc tgctgggcga gcccaaagtc ctgctgtttg
    14161 acgaaccgac aaccggcccc gaccctgcat cacgacaaat gttttacgaa gtcgtgcgcg
    14221 aactcaacgg gcgcggcgcg accgtattgc tcagtaccca cgcccttgcc gagttggacg
    14281 gacacgccga ccgcattgtc gtgatgaaaa acggcgttaa ggttgccgac ggcagtatgg
    14341 acgaattgca cgtccaaagc gggctgccgc tgaccgtcaa tatccgcctc aatgcgccgc
    14401 gcaccctcag cagccgctgg cagccgcttt cagacggcat ttcctaccgg gcgcaatgtc
    14461 aggccgaaga acgtatggaa ctgttgggcg aattgggcag cctgtccgac cttgcctatt
    14521 tcgacatcca cacgccgacg cttgacgaaa tgtacgcacg gttcttgaaa agggaggacg
    14581 tatgaacccc gtttggatta ttaccggcaa agaagcccgc gacagcctgc gtaaccgctg
    14641 ggtgcttgcc gccgtcctcc tgcttgccgc actcgccctt tctttgggtt tcctcggcag
    14701 ctcgcccacc ggttcggtca aggtcgatcc gctgaccgtt accgtcgtca gcctgtcgag
    14761 cctgtctatt ttcctgattc cgctgattgc gatgctgctt tcttatgacg cactgatcgg
    14821 cgaaatcgaa cgcggtacga tggcgttgct gttgagttat cccatttggc gcaaccaaat
    14881 ccttgccggc aagtttgtcg gacacctcat catcctcgcc cttgccacca cggcaggtta
    14941 cggattggca ggcattacgc tgcaacttgc caacggcggt ttcgacatcg ccgtttggaa
    15001 accctttgcg ctgttgattg ccgccagcgt gattttaggt gcggcatttc tgtctatggg
    15061 ctacttgatt agtgcaaagg tcaaagagcg ggggacggcg gccggtattt ccatcggcgt
    15121 atggttgttt ttcgtcgtca tcttcgatat ggcgcttttg ggtattctgg ttgccgactc
    15181 gaaacaggtc atcaccgcgc ccgtcgttga aacggtgctg ctgttcaatc ccaccgacat
    15241 ttaccgcctg ctcaacctga ccggttacga aaatacggct atgtatgcgg gtatggcggg
    15301 tttgagcgga caaatcggcc tgaccgtccc cgttttgctg accgcgcagg ttttatgggt
    15361 tatcattccg cttgttttgg cagccggaat ttttagaaag cgacgaatat gaagaaaacc
    15421 ctgttggcaa ttgttgccgt tttcgcctta agtgcctgcc ggcaggcgga agaggcaccg
    15481 ccgcctttac cccggcagat tagcgaccgt tcggtcggac actattgcag tatgaacctg
    15541 accgaacaca acggccccaa agcccagatt tttttgaacg gcaaacccga tcagcccgtt
    15601 tggttctcca ccgtcaagca gatgttcggc tataccaagc tgcccgaaga gcccaaaggc
    15661 atccgcgtga tttacgttac cgatatgggc aatgttaccg attggacgaa tcctaatgcc
    15721 gacacggagt ggatagatgc gaaaaaagcc ttttacgtca tcgacagcgg ctttatcggc
    15781 ggtatgggcg cggaagacgc gctgccgttc ggcaacaagg agcaggctga aaaatttgca
    15841 aaggataaag gcggcaaggt cgtcggtttt gacgatatgc ccgatgctta cattttcaag
    15901 taaatccaaa cagaaatgcc gtctgaacgt tgaagccaag gttcagacgg cattttttgt
    15961 cataggttca aacggggcag gttcgaacaa ctgccccatt ccttattcct gaccgggcgt
    16021 aacggcggaa tgaggtttac atcccgccgt aattcggtcc gcttgcgcct tcggggcaaa
    16081 tccaagtgat gttttgcgtc gggtctttga tgtcgcaggt tttgcagtgc acgcagtttg
    16141 ccgcgttgat ttgcaggcgc ggactgccgt tttcttcgat gatttcatat acgccggccg
    16201 gacaatagcg cgtttcggga gaggcgtatt ctttatagtt cacgtctatc atcgtttgcg
    16261 gattgttcag caccaaatgg tcgggctggt tttcttcgtg cgcgagattg gcaaggaaga
    16321 cgctgctcaa gcggtcaaag gtcaacacac cgtcgggttt cggataatca atcggcttgc
    16381 acacggcggc ttttttgagc tgctcgttgt ctttgccgtg atgtttcaag gtccacgggg
    16441 ctttgcctct gaaaatcatc tggtcgatgc cggtataaag cgagccgagg taaacgcccc
    16501 atttgaatga cggacggaca ttgcgcgcgg cataaagctc ttgatacagc cagctttgtt
    16561 caaacagttt ttgatagttt cccgcttctt tgccgctgtc gaagctttcc acttcttcaa
    16621 agttttccaa caaggggaac acggcttcgg cggcgagcat ggcggatttc atcgcggtat
    16681 gaatgccttt gatgcgcggc atattgagga atcccgccgc atcgccgatt aaaacgcccc
    16741 ccttgaacga gagcttcggc aggctttgca aaccgccttc aatcagcgaa cgcgcgccgt
    16801 aagcaatgcg gcggccgcct tcaaaggttt tgcggatttc gggatgggtt ttgaaacgtt
    16861 ggaactcttc aaacggcgac agatacgggt tttgatagtc caaaccgacc acgaagccga
    16921 cggcgacttt gttgtcgtcg aaatggtaaa taaacgcacc gccgtaggtt ttgctgtcca
    16981 gcggccagcc tgcgctatgc accaccaaac cgggctgatg ctgttcagac ggcacttccc
    17041 aaacttcttt gatgcccaag ccgtaagttt gcggctggct gttttggtcg agttggaaac
    17101 gttcgatgat ttgcttggaa agcgaacctc ggcaaccttc ggcaaacagg gtttgccgcg
    17161 cccaaagctc catgccgggc tggaaactgt cggtcggccc gccgtctttg cctacaccca
    17221 tattgccggt cgcaatacct ttgaccgagc cgtcttcgtg atacagcact tcggcggcgg
    17281 caaagcccgg atagatttcc acgcccatat tttccgcctg ctccgccaac cagcgcacga
    17341 cttcgcctaa gctgacgatg tagttgccgt gattgtcgaa attcggggta ataggcagat
    17401 tgaacgcttt tttctccgtc aggaacaaca ccttgtcctg agttaccgtg cgcgtcagcg
    17461 gcgcgccttt ttctttccag tcgggaatca gctcattcag cgcaatcgga tcgataaccg
    17521 cgcccgccag cgaatgcgcc cccgcctccg aacccttctc cgccacgcaa acgctgattt
    17581 cgcgcccgtt ctgttcggca agctgcttga gtttgatggc ggcggacaac cccgacgggc
    17641 ctgcgccgac aatcacgaca tcgtattgca tactgtcgcg ggtgatggat tctgtcatgg
    17701 cggttcctgt gtatttatta ttgaattgca aatccgtaat tatacaacgg gaacatatag
    17761 ttaccaaata caacaaaggt cgtctgaaaa ccatattttc ggttttcaga cgacctttgt
    17821 cgaaatttca ataagcacgc caccatttta cctgtccgac cgcaaactcc gtctgacgtt
    17881 tcggactgcg tgtgaaaaac gccttatccc caccggcatc cctccctttc ggcacgaccg
    17941 ccaaaatttt gcctgccaaa tttccctcac gggtttgcca agcctccaaa aactgcgccc
    18001 tgctcatcga aacatgaccc ggcgacgggt cggcaagcaa aaccgtattg ccatccactc
    18061 cgcgcaatac cgaaaaatgg tcgtctttgc ggtatttcag atacacgatg acggggattt
    18121 tcaactgcgc gagctgctcg aaagacaggg catagccctt cgcctcaaaa cccaaatcgg
    18181 gcataatgcg ccgcatatcc tcaaacgacg cgcgcatctg ctccttaccc agtttttcca
    18241 acacttcttc ttccgtcagc ttttgcccgt aaaaattgtt caaaagcgtc gccaccgaag
    18301 ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc cgcgctttcc
    18361 aactctgcaa tttgattttt ccgtaaacaa caggattatc gttaaacatc ggtgcagcat
    18421 tcaaacgata agacaa
//
LOCUS       DAWTCI010000038        18419 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWTCI010000038 DAWTCI010000000
VERSION     DAWTCI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18419
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.038"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            42..115
                     /locus_tag="WM481_001251"
     tRNA            42..115
                     /locus_tag="WM481_001251"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Cys,seq:gca)
     gene            216..305
                     /locus_tag="WM481_001252"
     tRNA            216..305
                     /locus_tag="WM481_001252"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:250..252,aa:Leu,seq:taa)
     gene            409..1461
                     /gene="sohB"
                     /locus_tag="WM481_001253"
     CDS             409..1461
                     /gene="sohB"
                     /locus_tag="WM481_001253"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFA9080566.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIIGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKI
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(1512..2378)
                     /locus_tag="WM481_001254"
     CDS             complement(1512..2378)
                     /locus_tag="WM481_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HFA9080567.1"
                     /translation="MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAV
                     IAGRVWQALLYALVVFLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLERDNHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(2389..2820)
                     /locus_tag="WM481_001255"
     CDS             complement(2389..2820)
                     /locus_tag="WM481_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFA9080568.1"
                     /translation="MNRLDQLGIRINLICNVFDKWIGQQDLNYNLFTVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQEGEQDRRERLLSLTEKGKVHAAPLTE
                     NAQEFSDKVFSTFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(3055..3924)
                     /locus_tag="WM481_001256"
     CDS             complement(3055..3924)
                     /locus_tag="WM481_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFA9080569.1"
                     /translation="MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGSTAELVRACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(4000..4911)
                     /gene="hisB"
                     /locus_tag="WM481_001257"
     CDS             complement(4000..4911)
                     /gene="hisB"
                     /locus_tag="WM481_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFA9080570.1"
                     /translation="MTKTQLHLNNFLTLAQEAGSLPKLAKLCGYRTPVALYKLKQRLE
                     KQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETRIAETA
                     SAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDIDIS
                     CKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLSGR
                     PGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFKAF
                     GRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(4928..6007)
                     /locus_tag="WM481_001258"
     CDS             complement(4928..6007)
                     /locus_tag="WM481_001258"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFA9080571.1"
                     /translation="MKSVRSFIRNDILAMSAYKITDVPPGFAKLDAMESPAHPFEGHE
                     ALMREWRAQLASAPIHLYPNPSGCGLQEALRSAFDIPDCAAVALGNGSDELIQFITML
                     TAKPGAAMLAAEPGFIMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAAIEASDGIVVVDEAYGAFNGDSFLPQAGRIPNLIVLRTL
                     SKIGFAGLRIGYATGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNAFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITIGSSAQNDAVLDVIRRLYR"
     gene            complement(6053..7342)
                     /gene="hisD"
                     /locus_tag="WM481_001259"
     CDS             complement(6053..7342)
                     /gene="hisD"
                     /locus_tag="WM481_001259"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFA9080572.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAIAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILVKDLNEACEISNYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(7339..8226)
                     /locus_tag="WM481_001260"
     CDS             complement(7339..8226)
                     /locus_tag="WM481_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFA9080573.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLSEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMTEETKNGRKQTRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGGKAYIRPKTE
                     QETLQTIPSPKITKENPKS"
     gene            complement(8318..8983)
                     /locus_tag="WM481_001261"
     CDS             complement(8318..8983)
                     /locus_tag="WM481_001261"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFA9080574.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIAPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSYLVVNKAALKTKYALLEPIIQSFGGAVKAKW
                     AFI"
     gene            complement(9062..9715)
                     /locus_tag="WM481_001262"
     CDS             complement(9062..9715)
                     /locus_tag="WM481_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFA9080575.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASAPAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKINQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            9991..10075
                     /locus_tag="WM481_001263"
     tRNA            9991..10075
                     /locus_tag="WM481_001263"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10025..10027,aa:Leu,seq:caa)
     gene            complement(10138..10245)
                     /locus_tag="WM481_001264"
                     /pseudo
     CDS             complement(10138..10245)
                     /locus_tag="WM481_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            10769..12496
                     /gene="ilvB"
                     /locus_tag="WM481_001265"
     CDS             10769..12496
                     /gene="ilvB"
                     /locus_tag="WM481_001265"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFA9080576.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELVETIKKAFQIAASGRPGPVVLDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNILSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRTDGKA"
     gene            12507..12998
                     /gene="ilvN"
                     /locus_tag="WM481_001266"
     CDS             12507..12998
                     /gene="ilvN"
                     /locus_tag="WM481_001266"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFA9080577.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            13065..13358
                     /locus_tag="WM481_001267"
     CDS             13065..13358
                     /locus_tag="WM481_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFA9080578.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYNLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAV"
     gene            13438..14451
                     /gene="ilvC"
                     /locus_tag="WM481_001268"
     CDS             13438..14451
                     /gene="ilvC"
                     /locus_tag="WM481_001268"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFA9080579.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRHGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVAELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGARLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(14570..15565)
                     /locus_tag="WM481_001269"
     CDS             complement(14570..15565)
                     /locus_tag="WM481_001269"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFA9080580.1"
                     /translation="MNLKNRHFLKLLDFTPEEITTYLDLAAELKDAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGADATYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFAQETVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKGVGFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPAGIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(15707..18292)
                     /gene="acnB"
                     /locus_tag="WM481_001270"
     CDS             complement(15707..18292)
                     /gene="acnB"
                     /locus_tag="WM481_001270"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFA9080581.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKNPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLVSPKYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVNILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKVGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVIPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRALERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKSDIPVRLWVAPP
                     TKMDAKELSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
BASE COUNT         4124 a         4484 c         5131 g         4680 t
ORIGIN      
        1 ctcgtttccc gctccaagtt ttgtagacaa gccagtttta gggcgagata gcaaagtggt
       61 tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc cgactctcgc ctccatatat
      121 cttacggcgg ggtggcagag tgtttatgct atgaggggtg caaccttcat ataggccggt
      181 taaaatccgc gcccccgcct ccacctttca caaatgcccg ggtggtgaaa taggtagaca
      241 caacggactt aaaatccgtc gggactaaac atcccatgcc ggttcgattc cggctccggg
      301 caccaagctg aaaatgacaa tgccgctcca agcggttatt tttttatctg tcggatgggg
      361 atggacaata ttgatatgtt catgcctgaa caagaggaaa tccaatcaat gtggaaagaa
      421 attttactga attacggtat tttcctgctc gaactgctta ccgtgttcgg cgcaattgcg
      481 ctgattgtgt tggctatcgt acagagtaag aaacagtcgg aaagcggcag tgtcgtactg
      541 acagattttt cggaaaatta taaaaaacag cggcaatcgt ttgaaacatt ctttttaagc
      601 gaggaagaga caaaacatca ggaaaaaaaa gaaaagaaaa aggaaaaggc ggaagccaaa
      661 gcagagaaaa agcgtttgaa ggagggcggg gagaaatctg ccgaaacgca aaaatcccgc
      721 ctttttgtgt tggattttga cggcgatttg tatgcacacg ccgtagaatc cttgcgtcat
      781 gagattacgg cggtgctttt gattgccaag cctgaagatg aggttctgct cagattggaa
      841 agtccgggcg gcgtggttca cggttacggt ttggcggctt cgcagcttag gcgtttgcgc
      901 gaacgcaata ttccgctgac cgtcgccgtc gataaggtcg cggcaagcgg cggctatatg
      961 atggcgtgtg tggcggataa aattgtttcc gctccgtttg cgattatcgg ttcggtgggt
     1021 gtggtggcgg aagtgccgaa tatccaccgc ctgttgaaaa aacatgatat tgatgtggat
     1081 gtgatgacgg cgggcgaatt taagcgcacg gttactttta tgggtgaaaa tacggaaaag
     1141 ggcaaacaga aattccggca ggaactggag gaaacgcatc agttgttcaa gcagtttgtc
     1201 agtgaaaacc gccccgggtt ggatattgaa aaaatagcga cgggcgagca ttggttcggc
     1261 cggcaggcgt tggcgttgaa cttgattgac gagatttcga ccagtgatga tttgttgttg
     1321 aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc aggagaagcg aagcctgatc
     1381 cagcgcattg gtttgcaggc ggaagcttcc gttgaaaagt tgtttgccaa acttgtcaac
     1441 cggcgagcgg atgtgatgta gtttgccgaa cggatggaaa tgccgtctga agtgtgttca
     1501 gacggcattt ttcaagttcc ggctttgatg ttccgttccg accactctat ccgttgtccg
     1561 atgtctttca aattggaata ttgttcgacc aatcgcggca catcgtccaa actcatggca
     1621 aacatccaca gataagtgcc gaccgaataa atatgccccg cgctgccgta gcctttgagc
     1681 gtcatcatca caaaagcaaa gccgaacaaa atacccatcg ccgcgccgac gcagagatag
     1741 ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg aaaccagtcc gtaatggcgg
     1801 tacagctgcc gctcgtcgcc ttttcggata aagtggttgt cgcgttccaa gctgttgttc
     1861 aggcggaaat acaggttttc gctgatggcg gcaaaacgtg gcaaaagcca taaaaacaac
     1921 gcaagtatgc ccaccgccga cacgccgacc caaaattcca gcaccagcag catgatgcac
     1981 gcgccgaata tggatacgac ggatgtcgcg gcaatcggca ggtgttcttc aaaaaagctg
     2041 acaaattcac gcgacagggc aacccgtgca gttaccgctg aatgcgggac ttgccgctgc
     2101 cgttgttcca acacaaccgg cacggcgatt tcggtataaa tccgcgtaaa cgtgcgcgta
     2161 tcggcaatcc gccgtgccgc accgaccagc cacatcaaaa atacaaccaa agcgtacagc
     2221 aacgcctgcc acaccctccc cgcaatcacg gcattaatcg cccagccgcc aaacaccgga
     2281 taccccagca tcaaaaggtt ttccagtccg accggggaaa atgtgccaat caatcgcttg
     2341 cggtgggttt tggctatgtg tttcaacatt ttccacatat ttgtccctct attttttatt
     2401 ttccgagatt gttttttcca tcacttccgc cagtgcatcc aaatcggcaa acagccgggt
     2461 tgtgcgcttg tcgccgaatg tggaaaatac tttgtcgctg aattcctgcg cgttttctgt
     2521 taaaggtgcg gcatggactt tgcctttttc ggtcagcgac agcaaccgtt cgcgcctgtc
     2581 ctgttcgcct tcctgccatt caatcaaccc ttgtccggca agggttttgc atacgcctga
     2641 aacggtctgt ttgggcagac tccactcttc tccgatgtgc ttttgcgtgc ggctgccttc
     2701 ggttgccagg gtataaagta cggtaaagag gttgtaattc aaatcctgct gcccgatcca
     2761 tttgtcaaag acattgcaaa tcaggttgat acggattcca agttggtcga gtcggttcat
     2821 aattggtctt gatattgact aaatttgtgc ggattatagt ggattaacaa aaaccggtac
     2881 ggcgttgcct cgccttgtcg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     2941 tttttgttaa tccactatat agtcataatc gggactgatt tcaagcgtgg ggcgataaaa
     3001 atgccgtctg aaacgctttc tggcttcaga cggcatattg tttggaaagg gaagtcagtg
     3061 ttctgccaat ttcaggtaaa cgccggaaac gtcctgttcg ccgtagccgg cttcaactgc
     3121 tttgcggtag ctggcagcaa cggtttcgac ggcgggcagg gtgttgcctg cctgttcaag
     3181 ctctttgacg gcgaggttaa ggtctttgga agcgtgtttg agtgcaaagg caggggggaa
     3241 ctcacggttt gcccatagtg atttttttgt ttgaaacata ggcgagtcca ttgccgagcc
     3301 gccgatggct tcgacgatgg tgtcggtatc gatgccgaac tgccgcgcca tcagcatcgc
     3361 ttcgctgtac gcttcgccga aaatgcctaa gagcgagttc aagacgagtt tcgcgcccga
     3421 gcctttgccg acatcgccga aatggaaggt ttttttgccg acaagggaaa atattttttg
     3481 cagcgggttt aaaacggctt cgctgccgcc gaacagaatc agcagtgtgc cgttggtggc
     3541 gggtccgacc gatccggaaa cgggtgcttc ggcaaactgt ccgcctgcgg cttcgacaag
     3601 tgctttgacg gcgaggtttt cggtcgggga gatggtgctc atgttgacga tgattttgcc
     3661 ggccaatccg tcgcggactc cgttcaggat gtcgcacacg gcggcatagt cggaaaccat
     3721 caggaaaatg acgggacagg cgcggacgag ttcggcggtg ctgccgtaaa cttttgctcc
     3781 tttggcggag atgggggcag ttttgtcggg cgagcggttg tatacgccga cttcgatgcc
     3841 gccgtccaag agccgcgtta ccataggcag acccatttgc cctaagccta cccagccgat
     3901 ttgtgtgtat gtttctgcgg acatagtgtt tctcctttgt tggggcgctg taatgccgtc
     3961 tgaaggcttc ctgcgcttca gacggtctgt ttggggatgt tatgcggtca gcgtgccttt
     4021 ggtcgagggg gtttgcccag ccattctcgg gtcgtgttcg actgccatac gcagggcgcg
     4081 cccgaaggct ttgaatacgg tttccgcctg atggtgggcg tttttgccgc tgaggttgtc
     4141 gatgtgcagg gtcatcatgc tgtggttgac gatgccgtgg aaaaattctt caaacaaatc
     4201 gacatcgaaa cgtccgatta gtgcgcgggt aaattcgatg ttgtacacga gtccggggcg
     4261 gccggaaagg tcgatgacga cgcggctgag ggcttcgtcg agcgggacgt aggaatgtcc
     4321 gtaacggcgg atgccttttt tgtcgccgag tgcctgccgg attgcttgtc cgagtgtgat
     4381 gccgatgtct tcggcggtgt ggtggtcgtc gatgtgcagg tcgcctttgc agctgatgtc
     4441 gatgtcaatc atgccgtggc gggcgatttg gtcgatcatg tgttcgagga agggaacgcc
     4501 ggtatccagc ctgcttttgc cgctgccgtc gaggttgatg gagacggtga tttgggtttc
     4561 gcaggtattg cggttgacgg taacgctgcg gcagccggca gcagatgcgg tttcggcaat
     4621 tcgggtttcg gcagttccgg tgctttctgc ggcggtttcg gggacagtgc gttcgcggtg
     4681 ttttctgtcg agccagcctt tgggtttgcc ggtgtgtttt tcgagttttg ccatcaggct
     4741 gggacggatg ccgcgcgcat cggggtcttc tgcctgcttt tcaaggcgtt gtttgagttt
     4801 gtaaagtgcg acgggggtac ggtagccgca gagtttggca agcttgggca gcgaacctgc
     4861 ttcttgggcg agagtcagga agttgttcag gtggagttgt gtcttggtca tagggattcc
     4921 gtttggttta tcggtagagc cggcggataa cgtcgaggac ggcatcgttt tgtgcggagc
     4981 tgcctatggt aatgcgcagg cagtgttcca aaagcggatg tgcgccgtgc agttttttga
     5041 cgaggatgcg gttttgtttg agtgtgtcaa acaacaaatc ggcatcgggt acgcgtatgg
     5101 taatgaagtt tgcctgactt ggaaaggcgt tcagacggca tattttgccc aattcggcga
     5161 acatccgttc gcgttcgttt ttcaggctgt cgatgttggc agaaatgatg ccgtagtgtt
     5221 gcagggcgag tttggcggtg gtcaggctca gttggttcat attgtagggc ggcaggattt
     5281 tttgcagttc gccgatgact tcgggacagc ctgtcgcata accgatacgc agtccggcaa
     5341 aaccgatttt gctgagggtg cgtaagacta tcaggttggg aatcctgccc gcctgcggca
     5401 ggaagctgtc gccgttgaat gcgccgtagg cttcgtcgac gacgacgatg ccgtctgaag
     5461 cttcgatggc ggcttcgatt tcggcacgcg tgaaacatac gccggtcggg ttgttcgggt
     5521 aggcgataaa ggtcagggca gggcggtgct tcctgacggc ttcgaggacg gcgggcaggt
     5581 tgagggtgaa atctccgttc agtggaacgc cgacataatc catgccgtac agcgcggcgt
     5641 tgtggcggta catgatgaaa ccgggttcgg ctgccaacat tgccgcgccg ggtttggcgg
     5701 tcagcatggt gatgaactgt atcagttcgt ccgaaccgtt gcctaaggcg acggcggcgc
     5761 agtcagggat gtcgaacgcc gaacgcaatg cttcctgcaa accgcagccg gaggggttgg
     5821 gatagaggtg gatgggggcg gatgccagtt gcgcccgcca ttcccgcatc agggcttcgt
     5881 gcccctcaaa aggatgggcg gggctttcca tcgcatcgag tttggcaaaa ccgggcggaa
     5941 catcggtgat tttgtatgcg gacatggcga ggatgtcgtt gcggatgaag gagcggacgg
     6001 atttcatcgt gtttccttaa tggttggaat atgcctgtac gccgtttcgg cattatttca
     6061 tacggaactc tgccgcgcgt gcgtgggcgg tcaggctttc gccgtgtgcc agcacgctgg
     6121 cggtttcgcc taatttttgc gcgccctgtt ccgaaacctg aatcaggctg gagcgttttt
     6181 ggaaatcata tgtccccaaa ggcgaggaaa agcgggcggt tcggctggtg ggcaacacat
     6241 ggttcgggcc ggcgcagtag tcgccgaggc tttcgccggt gtagcgtccc atgaaaatcg
     6301 cgccggcatg gcggattttt ttcgcccatt cctgcgggtt ttcgactgac agttccaagt
     6361 gttcggggga aatgtagttg gagatttcgc aggcttcgtt caagtcttta acgagtatca
     6421 tcgcgcccct gttgccgagc gaggcctcga tgatgtcgcg gcgcggcatg gtttcgatga
     6481 gcctgtccat ggcggcttct acttcgtcga gatacgcttg cgacgtgccg atgaggatgg
     6541 cttgggcaat ttcgtcgtgt tcggcctggc tgaacaaatc catcgccacc caatcggcgg
     6601 gtgtcgtgcc gtcggcgatg accagtattt cagacggccc cgccaccatg tcgatgccga
     6661 ccacgccgaa cacgcggcgt ttggcggcgg cgacgaaggc gttgcccgga ccggtgattt
     6721 tatcgacttg ggggatggtt tccgtgccgt aggcgagggc ggcgatggct tgcgcgccgc
     6781 cgacggtgaa gactttggtt acgccggcga cgtatgcggc ggcaagcacg atgtcgttgc
     6841 gttcgccttt cggtgtcggt acgaccataa tgatttcttt cacgcctgcg acgtgggcgg
     6901 gcatcgcgtt catgatgacg gaactcggat atgccgcctt gccgcctggg acgtaaatgc
     6961 cgacgcggtc aagcggggta atctgttgcc ccagcagcgt gccgtcttca tcggtgtagc
     7021 tccacgattc cattttttgg cgttggtggt agctttcgac acggcgggcg gcggtctgca
     7081 atgctgtctg aacgtcgttc ggaatgcgct cgaacgccgc gttcaaatcg gcttgcgtga
     7141 gtattaaatc atcgatgctt ttagcgtttg tctgatcgaa tttgttggtg tattcaatca
     7201 aagccgcatc gccgcgcttt tgcacgtcgg cgcaaatgtc ggcgacgatg cgttcggttt
     7261 cggggttttg cgcggtttca aaagccagca gggctttgag tccggcttgg aaatcgggcg
     7321 attgggtgtt gagttttttc atgatttggg attctccttt gttattttgg gacttgggat
     7381 cgtctgaagc gtctcttgct cggttttggg tctgatatac gcttttcctc ccccgctacc
     7441 aaacttcaat tcgggcaggc atttttgttg cagaccgagt ttggctaata cctcttcgct
     7501 acgagaggta gtacggaaac ggtaacgggt ttgttttctg ccgtttttgg tttcctcagt
     7561 cattgtttca aaaccgtcca cttcgtacgc actgacaaca gcgttttgca gaccggtgtg
     7621 aacgccgata acgtatttca cttttgaagc aacatcttta ccgataaccc agttgcccaa
     7681 cgtacgcgat ttgaggttgg catcgtcttg gttgtcgaaa aggtagtcta attcttcgtc
     7741 agtatctaaa tcgaaagcat tgtggatctt gatggcgaga atcagcccgt cggggttaat
     7801 ctcgctaagt gggagagaag agaatccaaa gcggcgatct agttcttcta cgctaatacc
     7861 acccggacca tgcccggcaa ttttattttt gagtttttta cccaagacag atttaacaaa
     7921 atgaattaag gcagattcgg cggcgagcgc ttcgacttca gtcagatgat agctgatgat
     7981 atagcgaccg agtttcttgc atttggagat ggctttgagt ttccgatcga taatctcgcc
     8041 ggagactggg tcttgtgaac gactagcaac ccattcatgc tcgaagatac gattaccgca
     8101 accttttccg atgtagagga ttttgtcttt tttcaggtcg gtcagacaat agacataaaa
     8161 ccgacgctcg ccgttattca aaacggaaag ggtcgaatct gagaattttt ttatttttgc
     8221 aaccatttta acttctccta tacagtttac tgccggtgat attttcagac ggcctgtcgg
     8281 cgggaacgga tagggtcgtc tgaaaacgca tctttattca aatgaatgcc cacttcgcct
     8341 tcactgcgcc gccgaacgac tgaataatcg gctccagcag cgcgtatttc gttttcaaag
     8401 cagccttgtt gaccaccagg tagctggaaa tgtcgacgat gtgttcgact gcctccaagc
     8461 cgtttgcctt caaggtgttt cccgtcgaaa ccaagtccac aatcgcatcg ctcaagccga
     8521 ccagcggcgc aagttccatc gagccgtaca gtttgataat gtccacatgg acacccttgc
     8581 cggcaaaatg agatgccgcg atttcaggat acttcgtggc aatcttcaga cggcatccgg
     8641 gttgcgaagc ggcttcgtaa tcaaaccctt tacgcacagc aaccatcatg cggcacttgg
     8701 caatctccaa atccaaaggt cggtaaagcc ccgtgccgcc gtgttcgatc agcacgtctt
     8761 tgcccgcaat gccgaagtcc gccgcgccgt agcggacata agtcggcaca tcggttgcgc
     8821 ggacaatgac aaggcggatg ttttcatggt tcgtcccgat aatcagcttg cgcgattttt
     8881 cgggctcttc agtcggagca atgccggcag cggcaagcag cggcagcgtc tcctcaaaaa
     8941 tgcgcccttt ggataaggcg atggtcaaag cattatcctg catggtgtgg atgtcctttg
     9001 tcggttgaaa caataatgcc gtctgaacag gcgccggcgg tgcagacggc atacggtttt
     9061 atcagagcag ggtccttaca tcggcagcaa tcgtttccgg ctcgcttccg taaggcgaga
     9121 aaatggcaac ctcaccgttt ttatcgataa gatacgcacc ggaagagtgg tcgaccaaat
     9181 agttttcgct gtcgtctttt tgattgattt tggcagaaac cacgcggtat tgctgcttga
     9241 tgaccggcag gttttgaccg cccgttgccg tcagaccgat aaagtccgga ttgaactgtt
     9301 tggcatactt gccgatgatt tcaggcgtgt cgcgttccgg atcgatgctg acgaacacca
     9361 ctttcacgtc cttagcctgc ccgcccaact gcttcaaagt gtcgctgtac gtcaaaagct
     9421 ctgtcgggca gacatcggga cagtgcgtaa agccgaaaga cagaatcacg accttgcctt
     9481 tcaaatcgct caggctgaaa ggcttgcctt cgccgtcggt cagcgtgaaa tcgccgccga
     9541 tgtcttcctt acgcatatcc gtaccgcgcg tttgcggctt tgccgcattt tccgcagccg
     9601 gcgcggatgc acttgaagaa gcggcttgcg ccgcactgtt gtcttgaggt ttgcaggcgg
     9661 caagcgcggc aagtacgaaa acgcccggca aaaaggaacg cggtacggaa aacatgacaa
     9721 tctcccaaga ataaatagaa aaacctgatt cgtacggaat ttgaatattt ccatcttaac
     9781 gcgtttggca gatttaatgt agtatttttt tacgttgtcc gtacggcagg aattgccgaa
     9841 tcatacagaa tctttggagc ggaaagaaca gtaagattaa aataataaaa attctattta
     9901 aaatcaaaaa caaaataaaa acagataaaa taatttcaaa aaaaggttta cagaatctca
     9961 aaatccatta taatggcaat ttcttcacca gcccaggtgg cgaaattggt agacgcaggg
    10021 gactcaaaat cccccgccgc aaggtgtgtc ggttcgagtc cgaccctggg caccacaacc
    10081 gcttttggag cggttatttt tttgtctatt gctgtttaat gaaatagata agactaaata
    10141 tagtggatta aatttaaacc ggtacggcgt tgcctcgcct tgccgtacca tctgtactgt
    10201 ctgcggcttc gtcgttttgt cctgatttaa atttaatcca ctatacaatc aatatattta
    10261 agtcgttgtt agccctcttg gttctgtcaa tgccgtccgg tgcaatgagc cattttcagg
    10321 aagtatggtt gggaatattt ttgatatatc aaaaataaaa tcaagttcaa tcaatatccc
    10381 gagtgaaacc ctgtaaattt taaaattctt gatactttca catctgcccc catgcaaact
    10441 gtcaacaatt tccatctttc aaaaaaggta ctcctgattt tattctttta aaatcaatca
    10501 ataatttctc cgctttcaaa aaaatttctc cgctttcaaa aaatattaaa agatgaaaga
    10561 tttttcttga ttgaactgtc ggtttgcgtt atcgttttta ctctttttca caaactctgt
    10621 gttcctttcc aattgattgg ataatctgtc tgttgccgtg ttcccccaat gcgcgtaccc
    10681 taaatcgctg cttggtggaa ttgcattaca ggtgctgtgg caaagcggtt tgtccctatt
    10741 ggtttgaaac cgtataaaag aggtcgttat gcagttatca ggcgcgcaaa tcatagtgca
    10801 gagtctcaaa gccgaaggcg tcgagtacgt tttcggttat cccggcggtg cggttatcga
    10861 aatctacgat gccctgttcc aactcaataa attcaaacac attcttaccc gccatgagca
    10921 ggcggcagta cacgcggcag atgcgtatgc gcgcgtcagc ggtaaggtgg gcgtggcatt
    10981 ggttacatcc ggcccgggcg ttaccaatgc gctgaccggt attgctactg cctatacgga
    11041 ttcgattccg atggtggtca tcagcggaca ggtaggcaat tccctgattg gtacggatgc
    11101 gttccaagaa gttgatacgg tgggtattac ccgtccgtgc gtcaaacaca atttcttggt
    11161 tacggacatc aatgagttgg tggaaaccat taaaaaggcg ttccaaattg ccgcaagcgg
    11221 ccgaccgggg cccgtggtgc ttgatgtccc gaaagatgtt acgcaggcga tggcgaaatt
    11281 cagctatcct caggaagaca tttttatccg ttcgtatcaa ccggttgttc aagggcatat
    11341 cggtcagatt aaaaaggccg tgcagatgtt ggcatctgcc aaacgcccgg tcgtctattt
    11401 tggcggcggc gtggtgttgg gtaatgcttc tgaagagctg acccgatttg tccgaatgac
    11461 gggtgctccg tgcacgggta cgctgatggg cttgggcgct tatccttccg gcgaccgcca
    11521 atttctcggt atgctcggta tgcacggtac ttacgaggca aaccttgcca tgcagaatgc
    11581 ggatgtcgtt cttgccgtag gtgcgcgttt tgacgaccgt gtggtttccg taccgtccaa
    11641 atttttcgag aaggcgaaga aggttatcca tatcgatgtc gatccttcca gcatcgccaa
    11701 acgcgtgaag gcggacattc cgattgtcgg cgacgtgaaa aacattttgt ccgagatggt
    11761 tgcgctgtgg caaaaacaag agtccgtgcc gtctgaagat gctttgggca aatggtggaa
    11821 aaccatagag gaatggcgtt cccgcgattg cttgtggttt gacaacggca gcgaaattat
    11881 caagccccaa tatgtgattc agaagcttgc cgagattacc ggcaattcgg caatcatcac
    11941 atcggatgta gggcagcatc aaatgtttgc ggctcaatat tatcctttcg aacgtccgcg
    12001 ccaatggctc aactccggcg gtttgggtac gatgggcgtg ggtctgcctt atgcgattgg
    12061 tgcaaaactt gccgccccgg atcaagacgt attctgtatt accggcgacg gctcgatcca
    12121 gatgaacatc caagagttgt ccacctgttt ccaatatcgg attccggtta acgtcattac
    12181 gctgaacaac ggttatctgg gtatggtacg ccagtggcag gaaatatatt acggcggccg
    12241 agagtcggaa acctatttcg attctttgcc cgatttcgtc aaacttgccg aggcatacgg
    12301 ccatatcggc atccgcgtgg acaagaaatc tgatgtggaa ggtgccttgt tggaagcatt
    12361 gaaccaaaaa gacaggctgg tgtttatcga cttcctgacc gaccagaaac agaatgtgat
    12421 gcctatggtc ggcaacggca aaggtttgga cgaaatggta cttccgccgc atatgcgtac
    12481 ggacggaaag gcgtaaggag aggcaaatgc gacatatctt atctgttctg atcgaaaacg
    12541 aatcaggtgc gatgagccgc gtggtcggtt tgttctctgc acgcgattac aatatcgatt
    12601 ctttggcggt tgcgccgacc gaagacaaaa ccctgtcacg gatgaccatc gttacccacg
    12661 gcgatgagca ggttatcgaa caaatcacca agcaactcaa taaattgatt gaagtgatta
    12721 aagtggtcga tttgaacgaa agccgttttg tcgaacgcga actgatgttg gtaaaagtcc
    12781 gtgccgccgg caaagaccgc gacgaatttt tacgcttgac cgaaatctac cggggcagca
    12841 tcatcgacgt aaccgaccgc agctatacga ttgaaattac cggctcgacc gacaagctgg
    12901 attccttttt ggaaacggtc ggacgcgccc aaattttgga aaccgtacgt acaggtgcgg
    12961 ccggcatcgg tcgcggtgag cgtattttga aaatttaacg ccgtaacctt tcagacggca
    13021 tggtatttga atgccgtctg aaaaacgaac ggcaggagag atttatgtcg aacattaaaa
    13081 ttgtcgcact ggttaccgtc aaaccggaat acacggaaac actggcagca cagtttaaag
    13141 aactggtcaa agccagccgt gcggaagagg gcaacatcag ctacaatctc catcaggaaa
    13201 tcggcaaacc gaaccgtttt gttttcgtgg aaaattggaa atcccaagca gctattgacg
    13261 aacataatgc cagcgcgcat ttccaagcct tcgtccagtc cgtcgacggc aaaaccgaag
    13321 cgttggaaat cgtattgatg aatgaagttg ccgtttaagc ggcacactct gtttaacccg
    13381 tccgaagccg ctcaacattt taaggcttcg acaaccattt accttaaagg aaatcaaatg
    13441 caagtctatt acgataaaga tgccgatctg tccctgatca aaggcaaaac cgttgccatc
    13501 atcggttacg gttcgcaagg tcatgcccat gctgccaacc tgaaagattc gggtgtaaac
    13561 gtggtgatcg gtctgcgcca cggctcttct tggaaaaaag ccgaagcagc cggccatgtt
    13621 gttaaaaccg ttgctgaagc gaccaaagaa gccgatgtcg ttatgctgct gctgcctgac
    13681 gaaaccatgc ctgccgtcta tcacgccgaa gttgcagcca atttgaaaga aggcgcgacg
    13741 ctggcatttg cacacggctt caacgtgcac tacaaccaaa tcgttccgcg tgccgacttg
    13801 gacgtgatta tggttgcccc caaaggtccg ggtcataccg tacgcagtga atacaaacgc
    13861 ggcggcggcg tgccttctct gattgccgtt taccaagaca attccggcaa ggccaaagac
    13921 atcgccctgt cttatgcggc tgccaacggc ggcaccaaag gcggtgtgat tgaaaccact
    13981 ttccgcgaag aaaccgaaac cgatctgttc ggcgaacaag ccgtattgtg cggtggcgtg
    14041 gccgagttga tcaaagcagg ttttgaaacc ctgaccgaag ccggttacgc gcctgaaatg
    14101 gcttacttcg aatgcctgca cgaaatgaaa ctgatcgttg acctgatttt cgaaggcggt
    14161 attgccaata tgaactactc catttccaac aatgcggagt acggcgaata cgttaccggc
    14221 cctgaagtgg tcaatgcttc cagcaaagaa gccatgcgca atgccctgaa acgcatccaa
    14281 accggcgaat acgcaaaaat gtttatccaa gagggtaatg tcaactacgc gtctatgact
    14341 gcccgccgcc gtttgaatgc cgaccaccaa gttgaaaaag tcggcgcacg actgcgtgcc
    14401 atgatgcctt ggattacggc caacaaattg gttgaccaag acaaaaactg attgttttca
    14461 aacgggactg cctacacatc gtgtaggcag tttgttatat ggataccgtc tgaacatcgt
    14521 gttcggacgg tatctgtgtt gcggatgaat ttaaacaggc acagttctgt cagtcgccca
    14581 gagccgcgac cattaccgct ttaatcgtgt gcatacggtt ttccgcctga tcgaacacga
    14641 tgccggccgg actttcgaat acttcttctg taacttccac accgttcagc ccgaaggttt
    14701 cgtaaatcca ttcgccgact ttggtttcgc ggttgtggaa ggcgggcagg cagtgcatga
    14761 atttgacttg cggattgccc gatgccgcca tcagttcggg cgtaacgcgg taatctttca
    14821 gcaaatcgat gcgttcctgc cagacttctt tcggctcgcc catgctgacc catacgtcag
    14881 tatgaatgaa accgacacct ttgacggctt catgcgcgtt ttcggtcagg gtaatttttg
    14941 caccggtttc tttggcggcg gcgtgtgcgg cggcgataat gccttcagac ggccacaggc
    15001 tttgaggtgc gccgatacgc acgtccatcc ccaattttgc ccctaaaatc agcagggaat
    15061 tgcccatgtt gtaacgcgcg tcgccgacgt aggcaaacgc ggtttggttc aaaggtttgc
    15121 cgctgtgttc gcgcatagtc agtgcgtcgg caagcatttg tgtgggatgg aactcgttgg
    15181 tcagcccgtt gaacacgggt acgcccgcat attttgccaa ttcttcgaca gtttcctgag
    15241 cgaagccgcg atattcgatg gcatcgtaca ttctgcctaa gacgcgcgcc gtgtctttga
    15301 tgctttcttt gtgcccgatt tggctggcgg acggttccag ataggttgca tccgcgcctt
    15361 ggtcgcgtgc ggcgacttca aacgcacagc gtgtgcgcgt ggatgttttt tcaaaaatca
    15421 gggcgatgtt tttccctttc atccgctgaa tctcgcgccc tgcctttttg gcgtctttca
    15481 actcggcggc aaggtcgagg taggtggtga tttcttccgg cgtgaagtcc aaaagtttca
    15541 gaaaatggcg gtttttcagg ttcactgtcg tttcctttat gtgggggcgt gaacgccgta
    15601 tttgcagcgt ttgcggaaag aagggttagc ggaatgttgc aatgccgtct gaaggttgtt
    15661 cagacggcat tgcggtcaat cccttactcc atacggatga cggggatcaa acattcacgg
    15721 tctcggctac ttcgttgtag ctgtcgattt cgttgaagtt catatagcgg tagattttat
    15781 cgccctgttc gttgatgatg ccgatattgg cttgatattc ttcaacggtc gggattttac
    15841 ccagtttgga gcaaatcgcc gccaactctg ccgagccgag gtaaacgaag gtgtttttac
    15901 ccaagcggtt cgggaagttg cgggtcgaag tggacatgac agttgcacct tcgcgtactt
    15961 gggcttggtt acccatacac aacgaacagc ccggcatttc catacgcgcg ccggcgcggc
    16021 cgagtacgcc gtagtgacct tcgtcggaca actctttcgc gtccattttg gtcggcggcg
    16081 ctacccacag gcggacgggg atgtcgctct tgccttccaa aagtttggag gcggcgcgga
    16141 agtggccgat gttggtcata caagaaccga tgaacacttc gtcgattttg gtgccggagc
    16201 gttcggacat aaagcatacg tcgtccgggt cgttcgggca ggcgataatc ggctctttga
    16261 tgtcgtccat gttgatttca atcacggcgg cgtattcggc atctttatcc gcttcgagca
    16321 actcgggatt tgccagccat ttttccatag ctttgatgcg gcgttccaaa gcgcgcggat
    16381 cttgatagcc gttggcaatc atatttttca tcaacacgac gttggatttc atgtactcga
    16441 taatcggctc tttattgagc ttcacggtac agccggcggc ggagcgttcg gcggatgcgt
    16501 cggtcaattc aaaggcttgt tccactttca aatcgggcag gccttcgatt tcgaggatgc
    16561 ggccggagaa gatgtttttc ttaccggctt tggcaacggt cagcaaacct tgtttaatcg
    16621 cgtaaagcgg gatggcgttc accaaatcgc gcagggttac gcccggttgc agcttgccgc
    16681 tgaaacgtac caatacggac tcgggcatat cgagcggtat tacgcccgtt gcggcggcaa
    16741 aagcgaccaa gcctgaacct gcggggaagg aaataccgat ggggaaacgg gtgtggctgt
    16801 cgccgcctgt accgactgta tcaggcaaca gcaggcggtt gagccacgag tggatgacgc
    16861 cgtcgcccgg acgcagggac acgccgccac gggtagaaat aaaggcgggc agttctttat
    16921 gggtttttac atcgacaggt ttcggatagg cagcggtgtg gcagaaagac tgcatcacca
    16981 tatcggcgga gaagcccaaa caagccaagt ctttcaactc gtcgcgggtc atcggcccgg
    17041 tcgtatcttg cgagccgacc gtcgtcatgc gcggttcgca gtaagtaccc ggacgcacgc
    17101 cttgtccttc gggcagaccg caggcgcgac cgaccatttt ttgcgccaag gtgaaaccga
    17161 ctttgctttc ggcaggcgct tgcggcaggc ggaatgcagt agaggcaggc agtttcaggg
    17221 cttcgcgcgc tttggcggtc agaccgcgac cgataatcag gttgatacgg ccgccggctt
    17281 gcacttcgtc cagcaatact tgtgatttca attcaaactc ggcaacagtc tcgccgtttt
    17341 tcacgatttt accttcataa ggaaggatat tgacgacatc gcccattttc agcgcggaaa
    17401 catcgacttc aatcggcagc gcgccggagt cttcttgagt gttgaagaaa atcggcgcga
    17461 ttttaccgcc caagcacacg ccgccgaagc gtttgttcgg cacgaacggg atgtcttcgc
    17521 cggtatgcca aatgacggag ttggtcgcgg atttgcgtga agaaccggta ccgaccacgt
    17581 caccgacgta ggcaaccgga tggcctttgg ctttgagttc ttccaacaat ttaatcggac
    17641 cgacttcgcc cggtttgtcg ggcgtgatgc cgtcgcgcgg gtttttcagc atggccagcg
    17701 cgtgcagcgg aatatcggga cgactccacg cgtcgggcgc gggagagagg tcgtctgtat
    17761 tggtttcgcc gtcaactttg aagacggtaa cggtgatttt ttcgggaact ttggcacggg
    17821 aggcgaacca ttcggcatct gcccaagatt gcaaaacttc ctgcgcgtat ttgttgcctt
    17881 tttcggcttt ttcctgaacg tcgtggaagg aatcgaacat cagaagcgta tgtttcaagc
    17941 ctttggcggc aatgggcgcg agtttgtcgt cgtccaagag ttcgattaag gcgtggatat
    18001 tgtaaccgcc gagcatcgta cctaagagtt cggtcgcata tttgggggaa accagcgggc
    18061 tggatgcgct gccttcggca acggcagcca ggaatgaggc tttgactttg gcggcatcgt
    18121 ccacaccggg cggaacacgg tgggcaagca gctcgaccaa gaactcgcct tcgcctgcgg
    18181 gcgggttttt cagcagctca accaaatcgg cggtttgctg cgcattcaaa gggagggcgg
    18241 gaatgccgag ggcggcgcgc tcggcggcgg ctttacgata ggcttctaac atctctttgt
    18301 tcctttttcc gtttttcttt tgtcgggttg caaatgatct gcgttaaata ttgtaaacaa
    18361 catttgtacc gctatcatag actagtttta acaaaaatgg aactgttatg acaggtaat
//
LOCUS       DAWTCI010000039        18331 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWTCI010000039 DAWTCI010000000
VERSION     DAWTCI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.039"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            6..81
                     /locus_tag="WM481_001271"
     tRNA            6..81
                     /locus_tag="WM481_001271"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WM481_001272"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WM481_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFA9080582.1"
                     /translation="MTEHTPEKKNVKVNQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WM481_001273"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WM481_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFA9080583.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WM481_001274"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WM481_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFA9080584.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMESGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WM481_001275"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WM481_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFA9080585.1"
                     /translation="MAKVSKRLKVLRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDSRKSDQVIRGSVVLPKGTGKTTRVAVFTQGVNAEAAKEAGADVVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKIGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGVRVDTSSVNN"
     gene            2470..2970
                     /gene="rplJ"
                     /locus_tag="WM481_001276"
     CDS             2470..2970
                     /gene="rplJ"
                     /locus_tag="WM481_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFA9080586.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFVELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3028..3399
                     /gene="rplL"
                     /locus_tag="WM481_001277"
     CDS             3028..3399
                     /gene="rplL"
                     /locus_tag="WM481_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFA9080587.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEAAGAKVEIK"
     gene            3590..7768
                     /gene="rpoB"
                     /locus_tag="WM481_001278"
     CDS             3590..7768
                     /gene="rpoB"
                     /locus_tag="WM481_001278"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFA9080588.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVYYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEQESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTNKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKLSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKERRKAGELREFLNKLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(7733..7873)
                     /locus_tag="WM481_001279"
     CDS             complement(7733..7873)
                     /locus_tag="WM481_001279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080589.1"
                     /translation="MDKGTVLSDGISFLNIVGLPRIPAEQLPLCRLKPVSVAFQIDIQ
                     TQ"
     gene            7921..12096
                     /gene="rpoC"
                     /locus_tag="WM481_001280"
     CDS             7921..12096
                     /gene="rpoC"
                     /locus_tag="WM481_001280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFA9080590.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLNMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLDVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENMEEGVTVAKQTDDVTGLSALVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDMMAANEKALEEGKEPARYENILLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12385..12798
                     /locus_tag="WM481_001281"
     CDS             12385..12798
                     /locus_tag="WM481_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080591.1"
                     /translation="MKVSQSAMNKLVIAVIASMGLTACAGGGSDSSMSVQPSVSEQLK
                     DNANVDAKDEKVIEYLKKSSLKDVPKELQAKVLKVKGDEYTGVRKQYAGKLGKGESVK
                     AMLFLDGEEPFSKEQLQKMDVYVNGKKYEGSKGGN"
     gene            12816..13445
                     /locus_tag="WM481_001282"
     CDS             12816..13445
                     /locus_tag="WM481_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080592.1"
                     /translation="MSEQKIEFYGADKEQNYALLKTWVYEQPYSVVRGYFGYSRKDGN
                     PIEEDGQNPEEIPFDLYLGDIRGVATDEDKLPKAGSFQYEGRAFGGNGVLSKESLDNH
                     NGVFRYTIDFDRRKGSGSIEGMEQYGKIKLEEAAIERIPYRESGSSLGLKDRVSYFGV
                     NEGVAMLEKDNEIKKYHLGIFGEAANEVAGAVSQEHKHQAVIGFGGEKK"
     gene            13685..13927
                     /locus_tag="WM481_001283"
     CDS             13685..13927
                     /locus_tag="WM481_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080593.1"
                     /translation="MKSKNIFFMGYLIASATFAEDIGVPVEPINVGSRAAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGGRSIP"
     gene            13839..14828
                     /locus_tag="WM481_001284"
     CDS             13839..14828
                     /locus_tag="WM481_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9080594.1"
                     /translation="MCRRFAMQCRLKFLKARQAAMFEAADLYRELLSERPDLVYPRFD
                     LGVMLFEDKQYREALVQLHRAEEVLPPDMRQLAREYIRQAEAVQAWHPSFNMNYEQTD
                     NVNNASLSRDIVINGRKWIKSEDSLPKRANGIRYELGIDRMFNMAGNHFARLGISGSG
                     VHYWNARDFSEQAFHAEVGYRYRNSRLEWGFRPFVKQNRLGNNRYTANTGIALDYSRR
                     LNEKWRSTQSFQYGRKQYHDEYLAKRYNSKTISVSGTFSYYAMSAWQLYGGISGMFDN
                     TVEKEQASRRYGVSLGTVKILDGGLGLKLGAGYTKRIFKAPATLIYNFTRRDD"
     gene            14835..14984
                     /locus_tag="WM481_001285"
     CDS             14835..14984
                     /locus_tag="WM481_001285"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9080595.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKNNSNMPAFYSHSGKGWFVS
                     MEKTY"
     gene            15163..15534
                     /gene="rpsL"
                     /locus_tag="WM481_001286"
     CDS             15163..15534
                     /gene="rpsL"
                     /locus_tag="WM481_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFA9080596.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            15652..16122
                     /gene="rpsG"
                     /locus_tag="WM481_001287"
     CDS             15652..16122
                     /gene="rpsG"
                     /locus_tag="WM481_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFA9080597.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKAAIEVFNEAIANSKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            16141..18246
                     /gene="fusA"
                     /locus_tag="WM481_001288"
     CDS             16141..18246
                     /gene="fusA"
                     /locus_tag="WM481_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFA9080598.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDSFTGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGEDLAEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4954 a         3968 c         4755 g         4654 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaatgttaa agtgaatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggagggttt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgccgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcga attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ttagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 tccccgtgtt tctggtttta ttggagggag ggctaataga cccacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gttggtcaac aggttcgtgt aaatgaagga ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgag aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggc aaagccaatc catccccccc agttggtcct gctttgggtc
     1201 aacgtggttt gaatatcatg gaattctgta aagcatttaa tgctgcaacc caaggtatgg
     1261 aatctggttt gccaattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac tcctccggct tctattctgt tgaaaaaagc cgccggtttg caaaaaggta
     1381 gttctaatcc tctgacaaac aaggtaggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtt cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggacg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagttcttc gctcttctgt tgaagctaac aaactgtacg caatcgacga
     1621 agcaattgcc ttggttaaaa aagctgctac cgctaaattt gacgaatctg ttgacgtatc
     1681 tttcaacttg ggcgttgatt ctcgtaaatc tgaccaagtt atccgtggtt cagtcgttct
     1741 gcctaaaggt actggtaaga caacccgtgt ggctgtattt actcaaggtg taaatgcaga
     1801 agctgctaaa gaggctggtg cggatgtcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg atgttgttat tgcttctcct gatgcaatgc gtatcgttgg
     1921 tcaattgggt accatcttgg gtcctcgtgg tctgatgcca aaccctaaaa taggtacggt
     1981 tacccctaat gttgccgaag cggtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcgttcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggacgc tatcgttaaa gccaaacctg ctgctgctaa
     2161 aggtcagtac ctgaaaaaag ttgctgtatc cagcactatg ggtttaggtg ttcgcgttga
     2221 tacatcaagc gtgaataact aatctaagaa attttcaagc aactctgttt ttgagttgct
     2281 tgaatttggg ctacttaaaa ttaagtagat gtccaagacc gtagggatcg taagatttaa
     2341 tcgtaactgc cctacgcaga cggtagtcct gaaacacatt gcaagattgc ttgtaagatg
     2401 tctttttagg ttaccgcgct ggtgggatat cgttttggta tcctgtttat aaacagtggg
     2461 aggtagacct tgagtctcaa tattgaaacc aagaaagtgg cggtcgagga aattagcgcg
     2521 gcaattgcta atgctcaaac cctcgtagtc gctgaatatc gcggtatcag tgtttccagt
     2581 atgactgagc ttcgtgcgaa tgcacgtaaa gaaggcgttt atttgcgcgt tctgaaaaat
     2641 actctggctc gtcgcgcagt gcaaggtact tcatttgtag aattggccga tcaaatggtt
     2701 ggtccgttgg tttacgctgc ttctgaagat gctgttgctg ctgctaaagt gttgcaccaa
     2761 ttcgcgaaaa aagatgacaa aattgtcgtt aaagccggtt cttacaatgg cgaagtaatg
     2821 aatgctgctc aggttgctga gttggcttct attccgagcc gcgaagagct gttgtccaaa
     2881 ctgttgttcg ttatgcaagc tcctgtatcg ggctttgcgc gcggtttggc tgctttggca
     2941 gagaaaaaag ccggcgaaga agctgcttaa tcgattttgt ttctgttaat caattatttt
     3001 ttaatacaat atttggagta aaatagcatg gctattacta aagaagacat tttggaagca
     3061 gttggttctt tgaccgtaat ggaattgaat gacctggtta aagcttttga agaaaaattc
     3121 ggtgtttctg ctgctgctgt tgcagttgca ggtcctgctg gtgccggtgc tgccgatgct
     3181 gaagaaaaaa ccgaatttga tgtcgttttg gcttctgccg gcgatcaaaa agtcggcgtg
     3241 attaaagttg tccgtgcaat tactggtttg ggtctgaaag aagctaaaga catcgttgac
     3301 ggcgcaccta aaaccattaa agagggtgtt tctaaagctg aagccgaaga catccaaaaa
     3361 caactggaag cagcaggcgc taaagtcgaa atcaaataat ttgatgcttc ttatgaaggc
     3421 tggcagtttt ctgccagcct tattttgctt cttaaaataa gcattaagta ttgtttacat
     3481 ttatttgcat agtttttatc aaatcattgc aaataaatgt aaatatcaga ttgatgcgta
     3541 ccgttgtttt cagacggcct attattgaaa attacttttc ggagtgtgta tgaactattc
     3601 gtttaccgag aaaaaacgta tccgtaagag ttttgcaaag cgggaaaatg ttttggaagt
     3661 tccttttcta ctggcaaccc aaattgattc ttatgcgaaa tttttgcagc tggaaaatgc
     3721 ttttgacaaa cgtaccgatg acggtctgca agcggcattt aattctattt tcccgattgt
     3781 gagccacaac ggttatgctc gtctggagtt tgtatattac acgctgggcg agcctttgtt
     3841 cgatatccct gaatgtcaat tgcgcggaat cacttatgct gcccctctgc gcgcgcgtat
     3901 ccgtttggtg attttggata aggaagcgtc taaaccgacg gtaaaagaag ttcgtgaaaa
     3961 cgaagtgtat atgggcgaaa ttccgttgat gaccccgagc ggttcttttg tgattaacgg
     4021 cacagagcgt gtgattgttt cccagttgca ccgttcgccc ggtgtgttct tcgagcatga
     4081 caaaggtaag acacactctt ccggcaaatt gttattctcc gcccgcatca ttccctaccg
     4141 tggttcatgg ttggattttg aatttgatcc gaaagatttg ctgtatttcc gtatcgaccg
     4201 ccgccgtaaa atgccggtaa cgattttgtt gaaggctttg ggctacaaca atgagcaaat
     4261 cttggatatt ttctacgaca aagaaacgtt ttatttgtct tcaaacggtg ttcaaaccga
     4321 tttggtcgca ggccgtctga aaggcgaaac tgccaaggtc gatatcttgg ataaagaagg
     4381 caatgtattg gttgccaaag gtaagcgcat tactgcgaaa aatatccgtg atattaccaa
     4441 tgcaggcctg acccgtttgg atgtagaaca ggaaagcctg ctgggcaaag cattggctgc
     4501 cgatttgatt gattcggaaa ccggcgaggt attggcttct gccaatgatg aaattacaga
     4561 agagttgttg gccaaatttg atattaacgg cgtaaaagaa attacgaccc tttacatcaa
     4621 tgagttggat cagggtgctt atatttccaa taccttgcgc acggatgaga ctgccggccg
     4681 gcaggcggcg cgtgttgcga tttaccgtat gatgcgtccg ggcgaaccgc ccaccgaaga
     4741 ggcggtcgag caattgttta accgcttgtt cttcagtgaa gacagctacg atttgtcccg
     4801 cgtaggccgt atgaaattta atacgcgcac atacgaacaa aaactgtccg aagcccaaca
     4861 aaactcttgg tacggccgcc tgttgaacga gacgtttgcc ggtgctgccg acaaaggcgg
     4921 ttatgtcctg agcgtcgaag atattgtcgc ctcgattgcg actttggtcg agttgcgtaa
     4981 cggccatggc gaagtggacg atatcgatca cttgggcaac cgtcgagtac ggtcggtagg
     5041 cgagctgact gaaaaccaat tccgcagcgg tttggctcgt gtggaacgtg ccgtaaaaga
     5101 acgtttgaat caggcggaat cagaaaactt gatgccgcac gacttgatta atgcgaaacc
     5161 cgtttctgcc gccatcaaag aattcttcgg ctccagccaa ttgagtcagt ttatggatca
     5221 gaccaatccc ttgtctgaag taaccaataa acgccgtgta tctgcattgg gcccgggcgg
     5281 tttgacccgc gaacgtgccg gcttcgaagt gcgggacgtg catccgaccc actatggacg
     5341 tgtatgtcct attgaaacgc ctgaaggtcc gaacatcggt ttgatcaact cattgtctgt
     5401 ttacgcgcgc accaatgatt acggtttctt ggaaacgcct taccgccgcg ttattgacgg
     5461 caaagtaacc gaggaaattg attacttgtc tgccatcgaa gaaggccgct atgtgattgc
     5521 acaggcgaat gccgatttgg attcagacgg caatctgatt ggcgatttgg ttacctgtcg
     5581 tgaaaaaggc gaaaccatta tggcaacgcc cgaccgcgtc caatatatgg acgtggcaac
     5641 cggtcaggtg gtatccgttg cggcatccct gattccgttc ttggaacacg atgacgcgaa
     5701 ccgcgcattg atgggtgcca acatgcaacg tcaggcagtg ccttgtctgc gtcctgaaaa
     5761 accgatggtc ggtaccggta tcgagcgttc cgttgccgtt gactctgcta ctgcaatcgt
     5821 tgcccgccga ggcggcgtgg tcgagtatgt cgatgccaac cgcgttgtga tccgtgtcca
     5881 tgacgacgaa gcgactgccg gcgaagtggg tgtcgatatt tacaatttgg ttaaattcac
     5941 ccgttccaat caatctacca acatcaacca acgtccggct gtcaaagcag gcgatgtttt
     6001 gcaacgcggc gatttggtgg ccgacggcgc gtccaccgat ttgggcgaat tggctttggg
     6061 tcaaaatatg accatcgcct tcatgccgtg gaacggttac aactacgaag actcgattct
     6121 gatttccgaa aaagtggctg cggacgaccg ctatacttcg attcacattg aggaattgaa
     6181 tgtcgttgcc cgcgatacca agctgggtgc ggaagacatt acccgcgata ttccgaactt
     6241 gtccgagcgt atgcaaaacc gtttggacga atccggtatc gtttacatcg gtgcggaagt
     6301 agaagccggc gatgtgttgg taggcaaggt aacgcctaaa ggcgaaaccc agctgacgcc
     6361 ggaagaaaaa ctgctgcgcg ccatcttcgg cgaaaaagcg tctgacgtaa aagatacttc
     6421 attgcgtatg cctaccggta tgagcggtac ggttatcgac gttcaagtct ttacccgcga
     6481 aggcatccaa cgcgacaaac gcgcccaatc cattatcgat tccgaattga aacgctaccg
     6541 tttggatttg aacgaccaat tgcgtatttt cgacaacgac gcattcgacc gtatcgagcg
     6601 tatgattgtc ggtcagaaag ccaacggcgg tccgatgaag ctggccaaag gcagcgaaat
     6661 cacgaccgaa tatctggcgg gtctgccgag caggcacgat tggtttgata tccgtttgac
     6721 cgatgaagat ttggccaagc agttggaact gattaaattg agcctgcaac aaaaacgcga
     6781 agaagcggat gagttgtatg agatcaagaa gaaaaaactg acccaaggcg acgagctgca
     6841 acccggcgta caaaaaatgg tgaaagtctt tatcgccatc aaacgccgtc tgcaagccgg
     6901 tgacaaaatg gcgggccgcc acggtaataa aggtgtggta tctcgcattc tgcctgtgga
     6961 agacatgccg tacatggcgg acggccgtcc tgtggacatc gtactgaacc cgttgggcgt
     7021 accttcccgt atgaacatcg gtcagatttt ggaagttcac ttgggttggg cggcaaaagg
     7081 tatcggcgag cgcatcgacc gtatgctgaa agagcgacgc aaagccggcg agctgcgcga
     7141 gttcttgaac aaactctaca acggcagcgg taagaaagaa gatttggaca gcctgactga
     7201 tgaagaaatc atcgaattgg cttccaatct gcgtaaaggt gcatctttcg cctctcctgt
     7261 attcgacggt gcgaaagagt ctgaaatccg cgaaatgttg aacttggctt acccaagcga
     7321 agatcctgag gttgagaaac tgggcttcaa cgacagtaaa actcaaatca cgctgtatga
     7381 cggccgttca ggcgaagcat ttgaccgcaa ggttacagtc ggtgtgatgc actatctgaa
     7441 actgcaccac ttggttgacg aaaaaatgca cgcgcgttct accggcccgt acagtctggt
     7501 tactcaacaa cctctgggcg gtaaagctca gtttggcggc caacgtttcg gtgagatgga
     7561 ggtttgggca ttggaagcat acggcgcggc atacacgctg caagagatgc tgactgtgaa
     7621 gtctgacgac gtgaacggcc gtaccaaaat gtacgaaaac atcgtcaaag gcgaacacaa
     7681 aatcgatgcc ggtatgcccg agtccttcaa cgtattggtc aaagagattc gctcactggg
     7741 tttggatatc gatttggaac gctactgata cgggtttcag acggcataag gggagctgtt
     7801 ctgcaggtat gcggggcagc ccgacaatgt ttaaaaacga aatgccgtct gaaagcactg
     7861 tacctttatc catatcgaaa atccgccacg cggtaaaaat atttccttca aggagcagaa
     7921 atgaatttgt tgaacttatt taatccgttg caaaccgcag gtatggaaga agagtttgat
     7981 gccatcaaaa tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtt
     8041 aaaaaacctg aaaccatcaa ctaccgtacg ttcaaacccg agcgcgacgg cttgttctgc
     8101 gccaaaatct ttggcccggt caaagactac gaatgcttgt gcggaaaata caaacgattg
     8161 aaatttaaag gcgtaacctg tgaaaaatgt ggcgtagaag ttaccctgtc caaagtgcgc
     8221 cgcgaacgca tgggccatat cgaattggct gcacccgttg cccatatttg gttcttgaaa
     8281 tccctgcctt cccgcttggg tatggtattg aacatgacgt tgcgtgatat cgaacgcgta
     8341 ttgtactttg aagcattcgt ggtgaccgat cccggcatga ctccgttgca acgtcgtcaa
     8401 ttgctgactg aagacgatta ctacaacaaa ctggacgaat acggcgacga cttcgatgcc
     8461 aaaatgggtg cggaaggtat ccgcgaattg ttgcgaacct tggatgtagc aggcgaaatc
     8521 gaaatcctgc gccaagagct tgaatcgacc ggttctgaca ccaaaatcaa aaaaatcgct
     8581 aaacgcttga aagtattgga agccttccat cgttccggta tgaagctgga gtggatgatt
     8641 atggatgtgc tgccggtatt gccgcctgat ttgcgtccgt tggttccgtt ggacggcggc
     8701 cgttttgcca cttccgattt gaacgatttg taccgccgcg tcatcaaccg caacaaccgt
     8761 ctgaaacgcc tgctggaact gcatgcgcct gacatcatcg tccgcaacga aaaacgtatg
     8821 ttgcaagaag cggttgactc gctgttggat aacggccgtc gcggtaaagc catgaccgga
     8881 gccaacaaac gtccgctgaa atcgttggca gatatgatta aaggtaaagg cggccgcttc
     8941 cgtcaaaacc tgttgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc
     9001 ccatacctgc gtctgcacca atgcggtttg ccgaaaaaaa tggcgttgga actgttcaaa
     9061 ccatttattt tccacaaatt ggaaaaacaa ggtttggcgt ctaccgttaa agctgcgaaa
     9121 aaattggtag aacaagaagt acctgaagta tgggatatct tggaagaagt catccgcgaa
     9181 catccgatta tgttgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgag
     9241 cctattctga tcgaaggtaa agcgattcag ttgcacccat tggtgtgtgc cgcatttaat
     9301 gccgactttg acggtgacca aatggcagta cacgttccat tgagcttgga agcgcaaatg
     9361 gaagcacgca cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg
     9421 attatcgtac cttcccaaga catcgtattg ggtctgtact acatgacccg cgaccgtatc
     9481 aatgccaaag gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg tgcataccat
     9541 accaaacagg tcgagctggg tacaaaaata accgtacgtc tgcgcgaatg ggtgaaaaac
     9601 gaagcaggtg agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg
     9661 ttgagcgaaa tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa
     9721 aaagaaattt ccaagctgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt
     9781 atcttcgccg accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc
     9841 attgccgttg acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat
     9901 gccgaagtta aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc
     9961 tacaacaaag tggtggatat ttggggtcgt gccggcgata agattgctaa agcgatgatg
    10021 gacaacctgt ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca
    10081 ttcaactcca tttatatgat ggcggactcc ggtgcccgtg gttctgcggc tcagattaaa
    10141 cagttgtccg gtatgcgcgg tttgatggcg aaacctgacg gatcgattat tgaaacgccg
    10201 attacctcaa acttccgcga aggtctgacc gtattgcaat actttattgc gacccacggt
    10261 gcgcgtaagg gtttggcgga taccgcgttg aaaaccgcga actccggtta cctgacccgt
    10321 cgtctggtag acgtaactca agacttggtc gttgttgaag acgattgcgg tacttcagac
    10381 ggctttgtca tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt
    10441 attttgggtc gtgttaccgc gtctgacgtt gtcgatccgt caagcggcga gactttggtt
    10501 gaagccggta cgttgttgac tgaaaaactg gtggatatga ttgaccaatc cggtgtcgat
    10561 gaagtcaaag tccgtactcc gattacttgt aaaacccgcc acggtctgtg cgcgcactgt
    10621 tacggccgcg acttggcgcg cggcaaactg gtcaatgccg gcgaggcagt cggcgtgatt
    10681 gcggctcagt ctattggtga accgggtacc cagttgacca tgcgtacgtt ccacatcggt
    10741 ggtgcggcat cccgtgcggc agcagccagc caagtcgaag ccaaatccaa cggtacggca
    10801 cgtttcagca gccaaatgcg ttatgttgcc aacaacaaag gcgagttggt tgtcatcggc
    10861 cgttcttgcg aagtcgtgat tcacgatgac atcggccgtg aacgcgaacg ccacaaagta
    10921 ccttacggtg ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg
    10981 gcaacttggg atccgcatac ccgtccgatg attaccgaac atgcaggtat ggtgaaattc
    11041 gagaacatgg aagagggcgt taccgttgca aaacaaaccg atgatgtaac cggtttgtcc
    11101 gctttggtag tgattgacgg taaacgccgt tcctctagtg cttccaaact gctgcgtccg
    11161 actgtgaaac tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg
    11221 gtttcgatgg cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt
    11281 aaaggcgacg tattggcgcg tattccgcaa gcctcttcca aaacccgcga cattaccggc
    11341 ggtctgccgc gcgttgccga gctgtttgaa gcacgcgtgc cgaaagatgc cggtatgctg
    11401 gcggaaatta ccggtaccgt ttccttcggt aaggagacca aaggcaaaca acgtctgatt
    11461 atcactgacg tggacggtgt agcatacgag actttgattt ccaaagagaa acaaattctg
    11521 gtacatgacg gtcaagtggt aaaccgaggc gaaaccattg tggacggcgc ggtcgatccg
    11581 cacgatattc tgcgtctgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg
    11641 caagaggttt accgcctgca aggtgtgaag atttccgata aacacatcga agtcatcatc
    11701 cgtcaaatgt tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt tattaccgga
    11761 gagcaggtcg aacgcggcga tatgatggcg gccaatgaaa aagctttgga agaaggcaaa
    11821 gagccggcac gttacgaaaa catattgctg ggtattacca aagcctccct gtccaccgac
    11881 agcttcattt ctgccgcatc gttccaagaa accacccgtg tgttgaccga agctgccatc
    11941 atgggcaaac aagacgaact gcgcggtctg aaagagaatg tcatcgtcgg tcgcctgatt
    12001 cctgccggta ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag
    12061 caggagactg ctgaaaccca agtaacggat gaataatctt cggcgcgttc attcaataaa
    12121 aaaccgcaag ccttgagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt
    12181 tcgtcatttt gaggcgtgtg gatataagcc cgataagtgt cccatatctt cgtgccgtca
    12241 aattatgcat cgaatagaac gagttaaatc cgaaggttat tgtgttaaat attgcaaaaa
    12301 tactttaaaa atttcgcacg gtgggagggt agaattccca gacttaaagc ttaagttttt
    12361 ttaacaaagt ttgggggagt ctaaatgaaa gtatctcaat cagcaatgaa taaacttgtt
    12421 attgctgtta ttgcttctat ggggctaaca gcgtgcgcgg gtggcggttc cgattcatct
    12481 atgtcggttc aaccaagcgt ttcggaacaa ctgaaagaca atgcaaatgt tgatgcgaaa
    12541 gatgaaaaag ttattgaata tctgaagaaa tcatcattaa aagatgtacc aaaagaattg
    12601 caagccaaag tattaaaggt taaaggtgat gaatatacag gagtgcgaaa acaatatgca
    12661 ggtaagctcg gtaagggtga atctgtcaag gcaatgctat ttctagacgg cgaagagccg
    12721 tttagtaagg aacaacttca gaagatggat gtatatgtta atggtaaaaa atacgaaggt
    12781 agtaaggggg ggaactagat gttctcccca aaggtttgag tgagcaaaag attgagtttt
    12841 acggtgcgga taaagaacag aattatgcgc tattaaaaac ttgggtttat gagcaaccgt
    12901 attctgtggt tagaggctat tttggctaca gtcgaaaaga cggtaatcct atagaagagg
    12961 atggacaaaa tcctgaagag attccttttg acttgtatct gggcgacatt agaggcgtgg
    13021 caacagatga agacaaatta cccaaggctg ggagctttca atatgaaggt cgggcatttg
    13081 gcggtaatgg ggttttatcc aaagaatcgt tagataacca taatggcgta tttagataca
    13141 caattgattt tgatcgtaga aaaggctctg gctcaattga aggaatggaa caatatggca
    13201 agatcaaatt agaagaagct gctattgaga gaatccctta tcgagagtcc ggctccagct
    13261 tggggttgaa agaccgggtt tcttatttcg gcgtgaacga aggggtggct atgttagaga
    13321 aggataatga gattaaaaag tatcatttgg gtattttcgg cgaagcggct aatgaagtag
    13381 caggtgctgt aagccaagag cataaacatc aagctgttat cggtttcggc ggcgagaaga
    13441 aatagagaaa gcactatttc tatttagtgc cgtttgtccg ggcaagatgc cgtctgaagt
    13501 ttcagacggc attttcttca aaaaacagtc cgccaaatcg aatactgcca agcggcaatg
    13561 caatacctca aaacagcaat aatttctttt aaaacagttg attcttactg aacataaatg
    13621 gccgggcaat acgaaaagaa aaatggcgtt gctgggcatt ttatttgaat ttgttatgaa
    13681 attgatgaaa tctaaaaata tcttttttat gggataccta atcgcttcgg cgacgtttgc
    13741 cgaggatatc ggcgtgcctg tcgaaccgat taacgtcggt agtcgggctg cgatgccgtc
    13801 tgaaggggaa agcctcgccc tcctgccgtt tgcagaggat gtgccgccgg ttcgcgatgc
    13861 aatgccgtct gaagttccta aaagcgcggc aggcggcgat gttcgaggcg gccgatctat
    13921 accgtgagtt gttgtcggaa agacccgatc ttgtttatcc ccgttttgat ttgggcgtga
    13981 tgctgtttga agacaaacaa tatcgtgagg cattggttca gttgcaccgg gccgaggagg
    14041 tgttgccgcc cgatatgcgg cagcttgcac gggaatatat ccgtcaggcg gaagcggtgc
    14101 aggcgtggca tccttcgttc aatatgaact atgaacaaac agataatgta aacaatgcgt
    14161 ccctttcacg ggacatcgtc atcaacggcc ggaaatggat aaaatcggaa gacagcctgc
    14221 ccaaacgcgc caacgggata aggtatgaat tgggtattga ccggatgttt aacatggcag
    14281 gcaatcactt tgcccgctta ggcatcagcg gttccggtgt gcattattgg aatgcccggg
    14341 attttagcga acaggcgttt catgccgaag taggctaccg ttatcggaat agccgtctag
    14401 agtggggatt ccggcccttt gtcaaacaaa accggctggg caataaccgt tatacggcaa
    14461 atacgggtat tgcgttggat tacagccgcc gtctgaatga aaaatggcgc agcacccaat
    14521 ctttccagta tggccgcaaa caataccatg acgaatattt ggcaaaacgt tacaacagta
    14581 aaacgatttc ggtgagcggt acgttcagct attacgctat gtcggcttgg cagttgtacg
    14641 ggggtattag cggtatgttt gataatactg tggagaaaga gcaggcttca cggcgttatg
    14701 gtgtaagttt gggtacggtg aaaatattag acggcggtct tggactgaag ttgggtgcgg
    14761 gttacacgaa acggatattt aaagcccctg caacattaat ttacaatttt actcggcgtg
    14821 atgattaaca tcggatgagc gcggctttat ggcataaaaa actgtcgtgg aaaggattta
    14881 caccccaaat aaatttccgt tacaacaaga acaacagcaa tatgcccgcc ttttattcgc
    14941 acagcggcaa gggatggttt gtcagtatgg aaaaaacgta ttgacagtat tttctccagc
    15001 cgtccgactg attgtgaggg atgtcggtaa atatttatcg gcaaacaaga aaatcatctt
    15061 ttttcttgtc gttatgcttg actgtctgct tgcaataaaa atataattcc actcttgccg
    15121 acatggtgtc ggcaagtatt taactcaaca ggacgagaaa atatgccaac tatcaaccaa
    15181 ttggtacgca aaggccgtca aaagcccgtg tacgtaaaca aagtgcccgc actggaagcc
    15241 tgcccgcaaa aacgcggcgt gtgcacccgt gtatacacga ctacccctaa aaaacctaac
    15301 tctgcattgc gtaaagtatg taaagtccgc ctgaccaacg gttttgaagt catttcatat
    15361 atcggcggtg aaggccacaa cctgcaagag cacagcgtcg tactgattcg cggcggccgt
    15421 gtaaaagact tgccgggtgt acgttaccac actgtacgcg gttctttgga tactgcaggt
    15481 gttaaagacc gcaaacaagc ccgttctaaa tacggtgcta agcgtcctaa ataattaccg
    15541 ggactcaaat aggcacgtcg gccgcctaag ctgaacaacg gccgagtaag tgaatactca
    15601 attgggtatt catgggaata gacccgactg aatagattaa aggaaattaa aatgccaaga
    15661 cgtagagaag tccccaagcg cgacgtactg ccagatccta aattcggtag cgtcgagttg
    15721 accaaattca tgaacgtatt gatgattgac ggtaaaaaat ccgttgccga gcgtatcgtt
    15781 tacggtgcgt tggaacagat tgagaaaaaa accggcaaag cagcaatcga agtatttaac
    15841 gaagccattg caaactccaa acctatcgtg gaagtgaaaa gccgccgtgt aggtggtgca
    15901 aactaccaag ttcctgttga agttcgtcct tcacgccgtc tggctttggc aatgcgttgg
    15961 gttcgcgacg cggcccgcaa acgtggtgag aaatccatgg atctgcgttt ggcaggcgag
    16021 ttgattgatg cgtccgaagg ccgtggcggt gcgttgaaaa aacgtgaaga agtacaccgt
    16081 atggctgaag ccaacaaagc attctctcac ttccgtttct aattttgaaa ggctaataaa
    16141 atggctcgta agaccccgat cagcctgtac cgcaacatcg gtatttccgc ccatatcgat
    16201 gcgggtaaaa ccacgacgac agaacgtatt ttgttctata ccggtttgac ccacaagctg
    16261 ggcgaagtgc atgacggtgc ggctactacc gactacatgg aacaagagca agagcgcggt
    16321 attaccatta cctccgctgc cgttacttcc tactggtccg gtatggcgaa acaattcccc
    16381 gagcaccgct tcaacatcat cgacaccccg gggcacgttg actttaccgt agaggtagag
    16441 cgttctatgc gtgtattgga cggcgcggta atggtttact gtgcggtggg cggtgttcaa
    16501 ccgcaatctg aaaccgtatg gcggcaagcc aacaaatacc aagttccgcg cttggcgttt
    16561 gtcaataaaa tggaccgcca aggtgccaac ttcttccgcg ttgtcgagca aatgaaaacc
    16621 cgtttgcgcg caaaccccgt acctatcgtc attccggtag gcgcggaaga cagtttcacc
    16681 ggtgttgtcg atttgctgaa aatgaaatct atcatctgga atgaagccga taaaggtaca
    16741 acctttacct atggcgatat tcctgccgaa ttggtcgaaa ctgccgaaga atggcgtcaa
    16801 aatatgattg aagccgcagc cgaagccagc gaagaactga tggacaaata cttgggcggt
    16861 gaagatctgg ccgaagaaga aatcgtaggc gcgttgcgtc aacgtacttt ggcaggcgaa
    16921 attcagccta tgctgtgcgg ttctgcattt aaaaacaaag gtgttcaacg tatgttggac
    16981 gcagttgtag aattgctgcc agctcctacc gatattcctc cggttcaagg tgttaatcct
    17041 aacactgaag aagccgacag ccgtcaagcc agcgatgaag agaaattctc tgcattggca
    17101 ttcaaaatgt tgaacgacaa atacgtcggt cagctgacct ttatccgcgt ttactcaggc
    17161 gtagtaaaat ccggcgatac cgtactgaat tctgtaaaag gcactcgcga acgtatcggt
    17221 cgtttggtgc aaatgactgc cgcagaccgt actgaaatcg aagaagtacg cgctggcgac
    17281 atcgcagccg ctatcggtct gaaagacgtt actaccggtg aaaccttgtg tgcggaaagc
    17341 gcgccgatta tcttggaacg tatggaattc cccgagccgg taatccatat tgccgttgag
    17401 ccgaaaacca aagccgacca agagaaaatg ggtatcgccc tgaaccgctt ggctaaagaa
    17461 gacccttctt tccgcgttcg tacagacgaa gaatccggtc aaaccattat ttccggtatg
    17521 ggtgagctgc acttggaaat tattgttgac cgtatgaaac gcgaattcgg tgtggaagca
    17581 aatatcggtg cacctcaagt ggcttaccgt gaaactatcc gcaaagccgt taaagctgaa
    17641 tacaaacatg caaaacaatc cggtggtaaa ggtcaatacg gtcacgttgt gattgaaatg
    17701 gaacctatgg aaccgggtgg tgaaggttac gagtttatcg atgaaattaa aggtggtgtg
    17761 attcctcgcg aatttattcc gtctgtcgat aaaggtatcc gcgatacgtt gcctaacggt
    17821 atcgttgccg gctatcctgt agttgacgta cgtatccgtc tggtattcgg ttcttaccat
    17881 gatgtcgact cttcccaatt ggcatttgaa ttggctgctt ctcaagcgtt taaagaaggt
    17941 atgcgtcaag catctcctgc cctgcttgag ccgattatgg cagttgaagt ggaaactccg
    18001 gaagaataca tgggcgacgt aatgggcgac ttgaaccgcc gtcgcggtgt tgtattgggt
    18061 atggatgatg acggtatcgg cggtaaaaaa gtccgtgccg aagtacctct ggcagaaatg
    18121 ttcggttact cgaccgacct gcgttctgca acccaaggcc gcgctactta ctctatggag
    18181 ttcaagaaat attctgaagc tcctgcccac atagctgctg ctgtaactga agcccgtaaa
    18241 ggctaatcag gcaaataggc cgtctgaaag gctgaaatga tttttcagat ggcattgttc
    18301 tttaatcgat ctttaatgta aaggaattag c
//
LOCUS       DAWTCI010000040        18272 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWTCI010000040 DAWTCI010000000
VERSION     DAWTCI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18272
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.040"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            275..973
                     /locus_tag="WM481_001289"
     CDS             275..973
                     /locus_tag="WM481_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFA9080599.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAAESKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARAGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1030..2034)
                     /locus_tag="WM481_001290"
     CDS             complement(1030..2034)
                     /locus_tag="WM481_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HFA9080600.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLSFAAADADFRRYFRAAFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVGLQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTADTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPAEFDEFYRRFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2104..4509
                     /locus_tag="WM481_001291"
     CDS             2104..4509
                     /locus_tag="WM481_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFA9080601.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAADTVAAEEADGRVAEGGAQGA
                     SESAQAFDLTLGSTCLFCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMQGQSQV
                     KVRAEGSVIIERDGAVLNTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQ
                     TGEAHNVRMETEQGGRRLQSVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVE
                     ADRGKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPY
                     YFNLAPNFDATFAPGIIGERGATFDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQA
                     KWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGS
                     LNAGLSVQKYQTLANQSGYKDEPYAIMPRLSADWHKNAGRAQIGVSAQFTRFSQDGRQ
                     DGSRLVVYPGIKWDFSNSWGYVRPKLGLHATYYSLDSFGGKASRSVGRVLPVVNIDGG
                     TTFERNTRLFGGGVVQTIEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYYG
                     NDRINAANSLSTAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKNPRSRSD
                     WVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGAGYRPAPGKVLNARYKYGRNEKIY
                     LQADGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGCW
                     GAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGRMDVAVPGYIPAHSLSAGRN
                     KRP"
     gene            4611..5612
                     /locus_tag="WM481_001292"
     CDS             4611..5612
                     /locus_tag="WM481_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9080602.1"
                     /translation="MMKIKALMIAAALLAAADVHAAPQKAKTAPAKAVKAAATAQKEA
                     APAQQQGGIRFSDGIAAVADNEVITNRRLAEAVAEAKATLPEDAQISESELSRQVLMQ
                     LVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSPTQRRELADNIIAEKVRQQAV
                     MQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSKNAAVGAESTIRK
                     IYDQARNGTDFAGLARRYSQDASAGNGGDLGWFADGVMVPAFEEAVHALKPGQVGAPV
                     RTQFGWHIIKLNEVRDAGTPEERIRNSVRQYIFQQKAGQATVNLLRDLHSGAYVDIR"
     gene            complement(6092..7972)
                     /locus_tag="WM481_001293"
     CDS             complement(6092..7972)
                     /locus_tag="WM481_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFA9080603.1"
                     /translation="MNIFYEESGQFKVAVVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPVYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLRTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQEGFEIKYFP
                     KGTGFPDLSLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGTKRVGIHIAAPS
                     LAVRQGGGMEQIIMQRLSTVYFPGGKITMLPENWITAFSLDAGAYRPAVSIYFDVDGE
                     FNVGEPTCKIEAVNIAANLRIQAIEPHFNAETGLDQAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENGLPGIFRVQPAGKVRMSTQSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQKSDAALFAALRDFDTAYAAYADFQRQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLTTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAAAPGGKTAGN
                     AV"
     gene            8128..8760
                     /locus_tag="WM481_001294"
     CDS             8128..8760
                     /locus_tag="WM481_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFA9080604.1"
                     /translation="MENVNRVPEQARYDAERRQADEALADVFPAVSIFGSARTPQDHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIALPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPVVLVG
                     KAFWSGLAEWINAQLLARGMISEGAASLFSISDDEDEIIAYLSEHGLQTA"
     gene            8832..10202
                     /gene="purB"
                     /locus_tag="WM481_001295"
     CDS             8832..10202
                     /gene="purB"
                     /locus_tag="WM481_001295"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFA9080605.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAAPEIAEVPPFSAETLAEIDKVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEARETVILPKLAEIIGKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDVTWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            10282..12147
                     /locus_tag="WM481_001296"
     CDS             10282..12147
                     /locus_tag="WM481_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFA9080606.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITNIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTIELSTVFLSNIYLGFRLGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRALRHISIILFLILTATSFLPAAFYTDILNQPNTYYLSTLRFPELLVGSLLAVY
                     GQTQNGRRQTENGKRQLLSLLCFGALLVCLFVIDKHDPFIPGITLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFISFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLMLVGYNLYSRGILKQEHLRPLPG
                     TPVAAENNFPETVLTLGDSHAGHLRGFLDYVGGREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAINQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPENTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSRGGAL"
     gene            complement(12286..12900)
                     /locus_tag="WM481_001297"
     CDS             complement(12286..12900)
                     /locus_tag="WM481_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFA9080607.1"
                     /translation="MNFTRLLNQVLSTVQKKGSTFSGSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGKTEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIAAAVGNDEALAA
                     ETYLAARLVCADLSRKETVFLARLSQALELDDNLVESLERQLGI"
     gene            complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="WM481_001298"
     CDS             complement(12936..15086)
                     /gene="dinG"
                     /locus_tag="WM481_001298"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFA9080608.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKAACPNRP
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGLSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALIDRLTGEFGLFIARIEQISAVWDLLSTVSIEGEEPLAKWIARRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGSFNLILRQTGLLWLPETTT
                     LALKSPFDFEKQGELYIPSIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALHLPGDYLPLLLVQGELPKAVLLQKHHQAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            complement(15269..16567)
                     /locus_tag="WM481_001299"
     CDS             complement(15269..16567)
                     /locus_tag="WM481_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFA9080609.1"
                     /translation="MPSETALLLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGSPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLARRGIPLYTAASPAGLVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRSVFGKTDLTPDNGTAHTDFNRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLPAPPPAALSDGIFLPQNGGETAAWRQWRRF
                     LEQAASYSVLKDFPSRKNTSLMGAYLSVGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHADDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPARPVAGV"
     gene            complement(16557..17468)
                     /locus_tag="WM481_001300"
     CDS             complement(16557..17468)
                     /locus_tag="WM481_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFA9080610.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHHNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGPLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            17576..17974
                     /locus_tag="WM481_001301"
     CDS             17576..17974
                     /locus_tag="WM481_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFA9080611.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            18023..18211
                     /locus_tag="WM481_001302"
                     /pseudo
     CDS             18023..18211
                     /locus_tag="WM481_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
BASE COUNT         3920 a         4997 c         5248 g         4107 t
ORIGIN      
        1 aaggggtgta acccttccaa atcagggcaa cgcatagggc ggtgctttat gtgccgtcct
       61 gtgtgttgaa acatcattgt tccccaacag ccgtatccgt ccggggcggc gcgtattgta
      121 cgacagaaac cgcttcggat ggcatcgggt ttgatgccgt ctgaagcggt ttcccgaaac
      181 gaaaacgtcc tttttcggcg gcaaactgta tcaacacgcg aaaatacaat aaaataccaa
      241 tatccgattt atttccaaca tcacggagag ccgtatgaaa tccagacacc tcgccctcgg
      301 cgttgccgcc ctgttcgccc ttgccgcgtg cgacagcaaa gtccaaacca gcgtccccgc
      361 cgacagcgcg cctgccgctt cggcagccgc cgccccggca ggactggtcg aagggcaaaa
      421 ctacaccgtc cttgccaacc cgattcccca acagcaggca ggcaaggttg aagtgcttga
      481 gtttttcggc tatttttgtc cgcactgcgc ccgcctcgaa cctgttttga gcaaacacgc
      541 caagtctttt aaagacgata tgtacctgcg taccgaacac gtcgtctggc agaaagaaat
      601 gctgccgctg gcacgcctcg ccgccgccgt cgatatggct gccgccgaaa gcaaagatgt
      661 ggcgaacagc catattttcg atgcgatggt caaccaaaaa atcaagctgc aagagccgga
      721 agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac ggcaaaaaag tccttgccgc
      781 ctacgaatcc cccgaaagtc aggcgcgcgc cggcaaaatg caggagctga ccgaaacctt
      841 ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa tataaagttg aatttgccga
      901 ctgggagtcc ggtatgaaca ccatcgacct tttggcggac aaagtacgtg aagaacaaaa
      961 agccgcgcag taggcccgtt tgaaaaatgc cgtctgaaac ttggttttca gacggcattt
     1021 tgattgggtt tagaacgtaa agcccgtttc cagttcttca tcgccgacca gttcgaccaa
     1081 gagcgcgtag agcggggcga gttcggcata acggcgcgat acgcggcgca gatagtttaa
     1141 gaaacgcggg atttccggac ggtatttgtc tttgccgtcg cggtagtaca ggcgtgcgaa
     1201 gatgcccgcg accttcaagt gccgctgcac gcccatccat tcgaaccggc ggtaaaactc
     1261 gtcaaactcg gcagggacag gcaagccggc agcccgcgcc ttttcccagt agcggataac
     1321 caaatccaaa acaaactctt cttcccactc gataaaggca tcgcgcaaca gcgacaccaa
     1381 atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct aaaacgcccg gcctgccgcg
     1441 cgtcagcatc aggttgcgga cgataaagtc gcggtgcacg tagactttgg gctgcgccaa
     1501 caagggcggc agcagcgtat cggcggtttg ctgccaaagt tggcgttgtt tgaatgttaa
     1561 ttcgcgcccc aattcttttg cgacaaacca ttccgggaac aggttgattt cgcgcagcat
     1621 cgtttcacgg tcatattcgg gcaaaacccc ttcacggctt gccttctgca atccgaccag
     1681 ctcgccgatg gcctccagca acagggcttt gtgtgccgct tcgccctgct cctgaagcat
     1741 cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc acaaacccca aatccgtgtc
     1801 cgcgtgcaat acctgcggca cattgaccat gtcaaacagc ttctgcacct tcaaataagg
     1861 cgcgacactc atcttgtcgg gcggcgcatc catacagacg acgctgccgc cgtctgaaaa
     1921 cgccgcacgg aaatagcggc ggaaatcagc atccgccgcc gcgaaggaca gatcgaagtc
     1981 ccgttcggga taaacggtct gaagccaatt tttcagttcg gtttgccgtt gcataacagt
     2041 atccgggcat ttcgggttac aataaacact attctaaccg gtaaaccgac ttgaggggcg
     2101 attttggctc gtttattttc actcaaacca ctggtgctgg cattgggctt ctgtttcggc
     2161 acgcattgtg ccgccgatac cgttgcggcg gaagaggcgg acgggcgtgt cgcagaaggc
     2221 ggtgcgcagg gcgcgtccga atccgcacaa gctttcgatt tgaccctcgg ttcgacctgc
     2281 ctgttttgca gtaacgaaag cggcagcccc gagagaaccg aagccgccgt ccaaggcagc
     2341 ggcgaagcat ccgtccccga agactatacg cgcattgttg ccgacaggat gcaaggacag
     2401 tcgcaggtta aggtgcgcgc ggaaggaagc gttatcatcg aacgggacgg cgcagtcctc
     2461 aataccgatt gggcggatta cgaccagtcg ggcgacaccg ttaccgtagg cgaccggttc
     2521 gccctccaac aggacggtac gctgattcgg ggcgaaaccc tgacctacaa tctcgatcag
     2581 cagaccggcg aagcgcacaa cgtccgtatg gaaaccgaac aaggcggacg gcggctgcaa
     2641 agcgtcagcc gcaccgccga aatgttgggc gaagggcgtt acaaactgac ggaaacccaa
     2701 ttcaacacct gttccgccgg agatgccggc tggtatgtca aggccgcctc tgtcgaagcc
     2761 gatcggggaa aaggcatagg cgttgccaaa cacgccgcct tcgtgttcgg cggcgttccc
     2821 cttttctata cgccttgggc ggacttcccg cttgacggca accgcaaaag cggactgctc
     2881 gtcccgtccg tatctgccgg ttcggacggc gtttcccttt ccgtccccta ttatttcaac
     2941 cttgccccca acttcgatgc cactttcgcc cccggcatta tcggcgaacg cggcgcgacg
     3001 tttgacggac aaatccgtta cctgcgtccc gattacagcg gacagaccga cctgacctgg
     3061 ctgccgcacg ataagaaaag cggcaggaac aaccgctatc aggcaaaatg gcagcaccgg
     3121 cacgacattt ccgacacgct tcaggcgggt gtcgatttca accaagtctc cgacagcggc
     3181 tactaccgcg acttttacgg cggcgaagaa atcgccggca acgtcaacct caaccgccgc
     3241 gtatggctgg attatggcgg cagggcggcg ggaggcagcc tgaatgccgg cctttcggtt
     3301 cagaaatacc agacgctggc aaaccaaagc ggctacaaag acgaacctta cgccatcatg
     3361 ccccgccttt ctgccgattg gcataaaaac gcaggcaggg cgcaaatcgg cgtgtccgca
     3421 caatttacac gcttcagcca agacggccgc caagacggca gccgactggt cgtgtatccc
     3481 ggtatcaaat gggatttcag caacagctgg ggctacgtcc gccccaaact ggggctgcac
     3541 gccacttatt acagcctcga cagtttcggc ggcaaagcat cccgcagcgt cgggcgcgtt
     3601 ttgcccgttg tcaatatcga cggcggcaca accttcgaac gcaatacgcg cctgttcggc
     3661 ggcggagtcg tgcaaaccat cgagccgcgc ctgttctaca actatattcc tgccaaatct
     3721 caaaacgacc tgcccaattt cgattcgtcg gaaagcagct tcggctacgg gcagcttttc
     3781 cgcgaaaacc tttattacgg caacgaccgc atcaacgccg ccaacagcct ttccaccgcc
     3841 gtgcagagcc gtattttgga cggcgcgacg ggggaggagc gtttccgcgc cggtatcggt
     3901 cagaaattct atttcaagga tgatgcggtg atgcttgacg gcagcgtcgg caaaaatccg
     3961 cgcagccgtt ccgactgggt ggcattcgcc tccggcggca taggcgggcg tttcaccctc
     4021 gacagcagca tccactacaa ccaaaacgac aaacgcgccg aacattacgc cgtcggcgca
     4081 ggctaccgcc ccgcccccgg aaaagtgttg aacgcccgct acaaatacgg gcgcaacgaa
     4141 aaaatctacc tgcaggcgga cggttcctat ttttacgaca aactcagcca gctcgacctg
     4201 tccgcacaat ggccgctgac gcgcaacctg tctgccgtcg tccgctacaa ctacggtttt
     4261 gaagccaaaa aaccgataga aatgcttgcc ggtgcagaat acaaaagcag ttgcggctgc
     4321 tggggcgcgg gcgtgtacgc ccaacgctac gttaccggcg aaaacaccta caaaaacgcc
     4381 gtcttttttt cacttcagtt gaaagacctc agcagcgtcg gcagaaaccc cgcaggcagg
     4441 atggatgtcg ccgttcccgg ctacatcccc gcccactctc tttccgccgg acgcaacaaa
     4501 cggccctgac cgtcggaaac ccggcaggag caccgttcct gcacaagata gcattccacc
     4561 gacaacccca aacccgccat caaaggcagg attcaaacga taaggaaaga atgatgaaaa
     4621 tcaaagccct gatgattgcc gccgcattgc tggcagcagc cgatgtccac gccgcaccgc
     4681 aaaaggcaaa aaccgcgccc gccaaagcgg tcaaagctgc cgccacggcg caaaaagaag
     4741 ccgcacccgc acaacagcag ggcggtatcc gcttttcaga cggcattgcc gccgttgccg
     4801 acaacgaagt catcaccaac cgccggcttg ccgaagccgt tgccgaagcc aaagccaccc
     4861 tgcccgaaga cgcgcagata agtgaatccg agctgtcccg acaggtgctg atgcagcttg
     4921 tcaaccaatc cctgattgta caggcgggca aacgccgcaa cattcaggca agtgaagcgg
     4981 aaatcgatgc cgtcgtcgca caaaatcccg ccctcaaaaa cctcagcccc acccaacgcc
     5041 gcgagcttgc cgacaacatc attgccgaaa aagtccgcca gcaggcagtg atgcaaaaca
     5101 gccgcgtgag cgaagctgaa attgatgcct tcctcgagca ggcgcaaaaa caaggcatca
     5161 ccctgcccga aggcgcaccg ttgcgccaat accgcgccca acacatcctg attaaagccg
     5221 acagcaaaaa cgccgccgtc ggcgcggaaa gtaccatccg caaaatctac gaccaggcgc
     5281 gcaacggcac ggatttcgca ggtttggcac gccgatattc ccaagacgca agcgcgggca
     5341 atggcggcga cttgggctgg tttgccgacg gcgtaatggt tcccgccttt gaagaagccg
     5401 tccacgcgct caaacccgga caggtcggcg cgcctgtccg cacccaattc ggctggcaca
     5461 tcatcaaatt gaacgaagtg cgcgatgccg gcacgccgga ggaacgtatc cgcaattccg
     5521 tgcggcaata catcttccaa caaaaagccg gacaggcaac cgtcaacctg ttgcgcgacc
     5581 tgcattccgg cgcgtatgtc gacatccgct aaggcggttt gaatccaaaa gtcataccga
     5641 tcgggcaaaa gtccgggcgg tatggctttt ttgaatttcg agttactttt acaccgccat
     5701 tcgtcattcc cgcgcaggcg ggaatctagc aacgaaaagt aacaggaatt tatcgggaat
     5761 ggccggagtt taaaggtctg gattcccgcc ttcgcgggaa tgacgattca gaagttacac
     5821 gaaactcaaa aaaaaaaacg aaaccgaacg aaccggattc ccgcttttac gggaatgacg
     5881 gcatatcggt ttccgtgagg acaaacctag attcccgcct gcgcgggaat gacgggattt
     5941 tagattgcgg gtatttatcg ggaatggcga tttaaaaatt acctaaaact aaacttaaaa
     6001 gcaactgaaa cttaaaaaac taaaatttcg cctgcgtggg aaggatggga tttcagattt
     6061 ctgtttttgt gggaatttgc cgtatcgggc ttcagacggc attgcctgcc gttttgcccc
     6121 cgggtgcggc tgccttgatg tagttgagcg agacaaactg cttctcggca tccaattcgg
     6181 tgattttaaa caatgcctgc gatttgggca gcgcgtcaaa cggaataccg gtcgcgcgcg
     6241 ttgtcagtgg cagaccttcg atgcggacga ggtcttcttt cagaatcgtc gcggtcagtt
     6301 cgctgatgcc ttgttgttgc aggtacacga ggctccagta ggcttccatc tgccgttgga
     6361 agtcggcgta ggcggcgtag gcggtgtcga aatcgcgcag ggcggcgaag agcgcggcat
     6421 cgcttttttg atacagcggc tcggcagtgt cgtcgatcag gctgatcagc tgcttttggt
     6481 tgatgtagtc ggcggcgcgg cgcagcggcg aggtaaacca gccgtaatgc tgcacgccca
     6541 tgccgatgtg cggctcggat tgggtgctca tgcgcacttt gcctgccggt tggacgcgga
     6601 aaatgccggg cagcccgttt tcgtctagca tttgcgccca agtgctgttg gcaagaatca
     6661 tcatctcgct gaccagcgta tcgatgggcg aaccgcgttc gcggcggacg acggagacgt
     6721 tgccttcttc atccaattcg atgctgtaat cgtattgcgg cgcgcggtcg ggttcgtatt
     6781 tgccgcgcgc tttttgcagg gcggtggcga attgatagaa ccaaatcagg tcttgatggt
     6841 gggcgaacat catttcgcct gcttggtcca agccggtttc tgcgttgaaa tgcggctcga
     6901 tggcttggat acgcaggttt gcggcgatgt tgacggcttc gattttgcag gtcggctcgc
     6961 cgacgttgaa ctcgccgtcc acatcgaaat aaatgctgac ggcagggcgg tatgcgcctg
     7021 catcgaggct gaacgcggta atccagtttt cgggcagcat cgtgattttg ccgccgggga
     7081 aataaaccgt actcaaccgc tgcataatga tttgttccat tccgccgcct tgtcggacgg
     7141 caagcgacgg cgcggcgatg tggatgccga cgcgcttcgt gccgttgccc aaatcggtca
     7201 ggctcaaggc atcgtccact tcggtggtcg attcgtcgtc aatggaaaag gcggtaacgt
     7261 cggccttggg caggtcgggc atttcgggaa gggagaggtc ggggaaacct gttcctttgg
     7321 ggaagtattt gatttcaaag ccttcttgca ggtattgcgg aatggacgta atgccgcccg
     7381 tttttttcgc cagttcgtag gcggaggttt tcagtgcgtc ggcggctttg gtaaaggctt
     7441 tgtaggtcag cgactgtttg tcgggcgcgt gtaagatggt tctcaaatcc gccgcgattt
     7501 cagacggcat ctcgccgcgt ttcaaggctt ccgcccaagc gtcgatttgc gcgtcttgct
     7561 gttttttgcg ttcgatggcg gcaagcgctt gtttcaaagt ttcttcgggc gcggctttga
     7621 acacgccttt ggcttttttg tagaaataca ccggcgcggc gtacagcgca atcaaagtcg
     7681 ccgccagctc ggttttggtc ggcgcatggc cgtaatattc ttcggcgatg gcttcggcgg
     7741 taaactcctc ttcgccgcat acttcccaca ataaatcggt gtcgatgtcc gccgcctgcg
     7801 cctgagcgtt ttccaaaaac gccgccatat cgccgtcaaa ctcggcaaag acattgtttg
     7861 ccttcacttt ggtgcgtttg ccgtgttggg tatcgacttg gtaggtagcg tcgttttttt
     7921 ggacgacgac ggcaactttg aactggccgg actcttcgta aaaaatattc atttttcgga
     7981 tttttctgtg gaaactcaag cgggcgattt tagcagatta ccgaaaatgc cgtctgaaaa
     8041 aaggttgggg gaaggcgggt gcggctttgc ggtgcttgcg ttgcgttttt gacggggtac
     8101 aatcgccgtt tttgaacgga ggacggaatg gagaatgtaa accgcgtgcc ggagcaggca
     8161 cgttatgatg ccgaacgcag gcaggcagac gaagcattgg cggacgtgtt tccggcagtc
     8221 agtattttcg gcagcgcgcg cacgccgcag gatcatgcgg attatgcgtt tgcctgccgt
     8281 ctggcgcggc ggctgtcgga ttcgggtatt gccgtcattt cgggcggcgg gccggggatt
     8341 atggaggcgg caaacaaggg cgcgtttgcc gggaagtcgg tttcggtagg gctgaacatt
     8401 gccctgccgc acgagcagaa accgaatccg tatcaggaca tcgccttgcg gttttcccgt
     8461 tttgccgaac gcaaggcagt gtttttccgc tattcccaag catatgtcgt gatgccgggc
     8521 ggcttcggga cgctggacga attgtttgaa atcctgacct tggtgcagac gggcaaagtg
     8581 ccgccgcgtc cggtcgtttt ggttggaaag gcgttttggt cgggcttggc ggagtggata
     8641 aacgcgcagc ttttggcgcg cggtatgatt tccgaagggg ccgcttcttt gttctccata
     8701 tcggacgacg aagacgaaat cattgcgtat ctgtcggaac acgggcttca gacggcatag
     8761 cgtcctgaga gtgatgtata attgcaaaca atttgtcaat ttttgatgtc ttttccgaac
     8821 aggatgccga tatgatcaac cccatcgcct cgctttcccc cctagacggc cgttatgccc
     8881 aatccgttga agcattgcgc ccgatttttt cagagtacgg actgatgaag gcgcgcgtca
     8941 aagtcgaatt aaactggctc aaagccctgg ccgccgcgcc ggagattgcc gaagtgccgc
     9001 ccttcagtgc cgaaacgctt gccgaaatcg acaaagtcat cgaaaacttt tcattagaag
     9061 acgcggccgc cgtcaaagcc atcgaagcca cgaccaatca tgatgtcaaa gccatcgaat
     9121 attggctgaa aaaacgtttt gccgaagtgc cggaagtcgc cgccgtgagc gagttcatcc
     9181 acttcgcctg caccagcgaa gacatcaaca acctgtccca cgccctgatg ctgcaagagg
     9241 cgcgcgaaac cgtcatcctg cccaaacttg ccgaaatcat cggcaaactc accgctatgg
     9301 cgcacgacct tgccgccgtc ccgatgatga gccgcaccca cggccagccc gccacaccga
     9361 ccactttggg caaagaaacc gccaatgtcg tgtaccgcct gcaacgccag ttcaaaatcc
     9421 ttcaggcgca agaattcctc ggcaaaatca acggcgcggt cggcaactac aacgcccaca
     9481 tggccgccta tcccgatgtg gattgggaaa cacactgccg caacttcgtc gaaatcagcc
     9541 tcggcctgac cttcaacccc tacaccatcc aaatcgagcc gcacgactat atggcggagt
     9601 ttttccaaac cctcagccgt gtcaacacca tcctgatcga ctttaaccgc gacgtttggg
     9661 gttatatttc attgggttat ttcaaacaaa aagtcaaagc gggcgaagtc ggttcttcca
     9721 ccatgccgca caaagtcaac cccatcgact ttgaaaactc cgagggcaac ctcggcatgg
     9781 caaacgccgt attgggcttt ttgtccgaaa aactgccgat ttcccgctgg cagcgcgacc
     9841 tgaccgacag caccgtattg cgcaatatgg gcgtgggcgt gggctatgcc gtgttgggtt
     9901 tcgccgccca cctgcgcggt ctgaacaagc tcgagccgaa ccctgccgcg cttgccgccg
     9961 atttggatgt cacttgggag ctgctcgccg agccgattca aaccgtaatg cgccgttacg
    10021 gtgtcgccaa tccttacgaa aaactgaaag acctgacgcg cggtaaaggc ggcatcacac
    10081 ccgaagtgct gaaaggcttt atcggatcgc tggaaattcc cgccgaagcc aaagccaaat
    10141 tgcttgagct gacccccgcg ctgtatgtgg gcaaggctga agcgttggcg aaacggattt
    10201 gagcgtttac tgaaaccgat gccgtccgaa cgcgcgttca gacggcattt ttaagataac
    10261 gggacatacg gggcgatatt gatgcaagct gtccgataca ggcctgaaat tgacggattg
    10321 cgggccgtcg ccgtgctatc cgtcattatt ttccacctga ataaccgctg gctgcccgga
    10381 ggattcctgg gggtggacat tttctttgtc atctcgggat tcctcattac caacatcatt
    10441 ctttctgaaa tacagaacgg ttctttttct ttccgggatt tttatacccg caggattaag
    10501 cggatttatc ctgcttttat tgcggccgtg tccctggctt cggtgattgc ttctcaaatc
    10561 ttcctttacg aagatttcaa ccaaatgagg aaaaccatag agctttctac ggtttttttg
    10621 tccaatattt atttggggtt ccgattgggg tatttcgatt tgagtgccga cgagaacccc
    10681 gtactgcata tctggtcttt ggcggtagag gaacagtatt acctcctgta tcctcttttg
    10741 ctgatattct gctgcaaaaa gacaaaatcg ctacgggcgt tgcgccacat cagcatcatc
    10801 ctgttcctga ttttgactgc cacatcgttt ttgccggccg cgttttatac cgacatcctc
    10861 aaccaaccca atacttatta cctttcgaca ctgaggtttc ccgagctgtt ggtgggttcg
    10921 ctgttggcgg tttacgggca aacgcaaaac ggcagacggc aaacagaaaa tggaaaacgg
    10981 cagttgcttt cattactctg tttcggcgca ttgcttgtct gcctgttcgt gatcgacaaa
    11041 cacgatccgt ttatcccggg aataaccctg ctccttccct gcctgctgac ggcgctgctt
    11101 atccggagta tgcaatacgg gacacttccg acccgcatcc tgtcggcaag ccccatcgta
    11161 tttgtcggca aaatctctta ttccctatac ctgtaccatt ggatttttat ttccttcgcc
    11221 cattacatta caggcgacaa acagctcgga ttgcctgccg tatcggcggt tgccgcgttg
    11281 acggccggat tttccctgtt gagctattat ttgattgaac agccgcttag aaaacggaag
    11341 atgaccttca aaaaggcatt tttctgcctt tatctcgccc cgtccctgat gcttgtcggt
    11401 tacaacctgt attcaagagg gatattgaaa caggaacacc tccgcccgct gcccggcacg
    11461 cccgttgctg cggaaaataa ttttccggaa accgtcttga ccctcggcga ctcgcacgcc
    11521 ggacacctgc ggggttttct ggattatgtc ggcggcaggg aagggtggaa agctaaaatc
    11581 ctgtccctcg attcggagtg tttggtttgg gtggatgaga agctggcaga caacccgttg
    11641 tgccgaaaat accgggatga agttgaaaaa gccgaagccg ttttcattgc ccaattctat
    11701 gatttgagga tgggcggcca gcccgtgccg agatttgaag cgcaatcctt cctgataccc
    11761 ggattccaag cccgattcag ggaaaccgtc aaaaggatag ccgccgtcaa acccgtctat
    11821 gtttttgcaa acaacacatc aatcagccgt tcgcccctga gggaggaaaa attgaaaaga
    11881 tttgctataa accaatatct gcgccccatt caggctatgg gcgacatcgg caagagcaat
    11941 caggcggtct ttgatttggt taaagatatt cccaatgtgc attgggtgga cgcacaaaaa
    12001 tacctgcctg aaaacacggt cgaaatacac ggacgctatc tttacggcga ccaagaccac
    12061 ctgacctatt tcggttctta ttatatgggg cgggaattcc acaaacacga aagcctgctc
    12121 aagcattccc gaggcggcgc attgtagtag cctgccttct tgccggatat tgcctttggc
    12181 agcctatgcc gctgtttgcc ctttgaggcg gcttttattt tgccgttttg aggccggggt
    12241 cgggaataac cgttttttga tgattttccc tccccggctg tgtcatcaaa tccctagttg
    12301 cctttccaaa ctctccacca gattgtcatc cagttccaaa gcctgcgaca ggcgggcgag
    12361 gaagacggtt tctttccgcg acaaatcggc acagaccaac cttgccgcca gataggtctc
    12421 cgccgccaac gcctcatcgt tgccgacggc ggcggcgatg tccccgatgc ttgcgggcag
    12481 gcggtattcg gcggcgagcc atgcggcagt ttcggggtct gtgccgcttt cctgttcgat
    12541 agtccggcgt tcggcttcgt ctatcatgcc gtctgaagcg gcggcggcta tcatggtacg
    12601 caatacggtg cggctgtatg tttcttcggt ttttccggca ggttggaaat cgctttgtgt
    12661 tacggttgcc cgccctttgt tttgctgcca catctgatag ccccggtagg cgaggtagcc
    12721 caaagcggcg gtcgaaccga ttttggtgat ggttttgcgg tttttaccgt tcagcagcat
    12781 ggaggcgaca ccggcaacca gcgcgcctcc gccgaatgaa ttgagcgggc tgccggagaa
    12841 tgtgctgcct tttttttgaa ccgtgcttaa gacttggttg agcaaccggg tgaagttcat
    12901 gatttttcct ttctgttgcg ggaagggcgg tatgtttacc ctatcctttt aaacggtggc
    12961 aggccggcca ataattgttg gccgtaccgc tgcgttttga tgcggttgtc gaggatggtt
    13021 acgcggccgt agtcttgttc ggtgcggatg aggcggccga cggcctggat gagtttgatg
    13081 ccggcttcgg ggacggtgat ttcgatgaag gggttgccgc cgcgttgttc tatccaacgg
    13141 ttttgggttt tttcgatggg gttgtcgggc atggcgaagg ggagtttggc gatgatgact
    13201 tgcacgcagg cggtgccggg caggtcgagc ccttcggcga agctgtcgag tccgaagatg
    13261 atgctggctt tgccttcttc tatggcttgg tggtgttttt gcaggaggac ggctttgggt
    13321 aattcgcctt gtacgagcaa gagcggcagg tagtctccgg gcaggtgcag ggcgacatcc
    13381 tgcatttgtt tgcgcgagga aaacaggacg agtgtgccga tggcttcggt gggcgaaatc
    13441 agcttgggca gccattcgat gacggcggcg gtgtgggctt cggggtcttt ggggctggcg
    13501 tatatggagg ggatgtagag ttcgccctgt ttttcaaagt caaaggggct tttgagggcg
    13561 agggtggtgg tttcgggcag ccacagcagg ccggtttggc gcaaaatcaa gttgaagctg
    13621 ccgagcgatt gcagggtggc cgaagtcagc accgcgcctg ccgcgcgccg ccacaggctg
    13681 ttggcaaggt gggacgcgct gctgatgggg ctggcattga aaatatagtc gtttttgtcg
    13741 tcggcacggc gggctatcca tttcgccaac ggctcctcgc cctcaatgga aacagtggag
    13801 agcaaatccc aaaccgcgct gatttgttcg atacgggcga taaaaaggcc gaactcgccg
    13861 gtcaggcggt cgatgagcgc accgtcctgt tctttttcgc ggcgtgcggc ggaaagcgca
    13921 tcgttcagcc cgacaacgtg tttgagcagg ctgcgtgcag caatggccgt attggaaacg
    13981 gtggtttcga ggccttcggg gattttgccg tcttcccaca gccaagtcgg ttcgctgttg
    14041 gttcgtctgt cgttttcaga cagccccata cttaaagacg gctcttccgc caaatggaat
    14101 tgccattcgt gcaggctgtc gagcaaggat gcggcggctt cgtcggcaag gttggcaagt
    14161 tcggctttat cggtaagcgc ggcaattttg ccggtaagct gcggcagttt ttccagcgtc
    14221 caaacggcaa tattccatga atgttcggcg gcaaaacggc tgagggcttt tttgggcagg
    14281 tggtgcgctt cgtctatgca atagaaactg ttttcgggcg caggcagaat cacgccgccg
    14341 cccatgctga tgtcggcaag cagaagatcg tggttggcaa caacgacatc gacggtttcc
    14401 aacatatcgc gtgccaggta aaaagggcat tccggacggt tgggacaggc ggctttcagg
    14461 cagccgtggc ggtcgttggt cactttgagc caaatcgcgt catcgatttt ttccggccaa
    14521 gcgtcgcggt cgccgttgaa ccgtcgggcg gaaaattcgt cggcgatgtc gcgcagcagc
    14581 ttcaattctt ccggcttggg tttgctgtcc cacaagacgg cgggggcttc aaagccgagc
    14641 aggttttgct gggcgttgct ttgcgtcagt tgatagagtt tgtaggggca gagatagcgg
    14701 ccgcgccctt tggcaagtgc gaaggtcagt tccaaaccgc ttttttcgac cagaaacggc
    14761 aggtcgcggt ctaccaactg ctcctgcaag gcaaccgtcg cgctgctcac aatcagccgc
    14821 ttgccgcgtg tttgcgccat gatgccgccc gcaagcaggt aggcaagcga tttgcccacg
    14881 ccggtcggcc cttcgattac ggcaatgctt tcgccctcgc gcttgggcgg ctcgccgcct
    14941 tcttcgcgcg tcaacgtccg cgaaaaagcg ttggcaaccg ccgcaatcat ttcccgctgc
    15001 gaagcacgcg gacggaaacc gggcaggttt ttgccgatat tttggtaatg gtcgcggatg
    15061 gcgttttttt ctaaatcggt gagcatagta tcggtgggcg gatgatttgg ggtcatttta
    15121 acattgcgcg gatgcggtac ggtatcgttg tcggacggtg gttcgggcat atctgattat
    15181 tgaaagtaaa aaaaaacgga agatattggg tcatccgtac aaaatgccgt ctgaagcggg
    15241 aaacgggctt cagacggcat tgccgcgctt atacgccggc gacggggcgg gcgggatagc
    15301 cgtgggtatc gaccgaacag gcaaaccgcc aaggcgtgtg gacggtgtcg gcggacaggt
    15361 gggcgagttc gggaatgtgc cgtttgataa aagtaccgtc cgggtcggta cggtatgagg
    15421 cggcggcaat gtcggggcag gtgtgccgtg aggcggcaag ccgccagttg ccttggttga
    15481 ttgccgcatc gaaatcggtc agctgccggg caaaccatat ctcgccttcg cggcggggga
    15541 ggtttaaaac gtggcagaaa aaatccgcgc tcaagcgtct cagggcgggg tggaggctgc
    15601 cggttttgtg caaacagcgc atcgcggcat cgataatcgg aatgccggtc cggccctgct
    15661 gccaaagcgt caggcgcagg gtgtgttccg gattgccgtc tgaagggtcg tcatccgcgt
    15721 gctgcaaggc aagttgaagg aaaaaatcgc ggcggatgat gttgtccgcc cacgcgttca
    15781 gacggcgttc gaggctttcc cgcgcgagca ggcgcggcga gatgcagccg acactcaaat
    15841 acgcgcccat cagcgaagtg tttttgcgcg aagggaaatc ctttaaaacg gagtaggaag
    15901 ccgcctgttc gagaaaccgc cgccactgcc gccaagccgc cgtttcgccg ccgttttgcg
    15961 gcaggaagat gccgtctgaa agcgcggcag gcggcggggc gggcaggttt tcggggaagg
    16021 gttggcggta tgccgcgaat aggtccgaac cggcgggggg ctgcttggaa aagcggtcga
    16081 gccatacttc gcggtagcgg ttgaaatcgg tgtgtgccgt accgttgtct ggtgtcaggt
    16141 cggttttgcc gaaaacggaa cggtcgttga cgaaggttaa cgcgatgccg tgtttgtcca
    16201 attcgtgcca aagggcgttg tcggcgagtt tgtcggcaaa agtatgggat tcgtcggcga
    16261 tgacggcgcg gatattgagg cggacggcga gccggacaag cccggcaggc gatgccgccg
    16321 tgtagagcgg gatgccgcgc cttgccagcc cttgggcgag ttcggcggcg gattggcggt
    16381 agaacgcggc gcggcgcggg ttgtctgttt cggcatcgtc aatccaaatg ccgataacgg
    16441 gcgaaccttc ggcaacggcg gcgcataagg cggcgttgtc gcggatgcgg aggttttggc
    16501 ggaaccagac gagcgtgtgc gcggcgcgcg tgtccgcata aaggaggagg gcggtttcag
    16561 acggcatttc ggcagccttt cctgctggcg attttttcgt tcagaaaatc gatgaaactg
    16621 cggactttcg cactcaaaaa tgccctgtcc gcataaacgg cgttcagccg gtcggtcggg
    16681 acggcgtatc cgggcagcag cctcaccagc gggccgcagc gcaaatcgtg ttccgccgcc
    16741 caaagcggct gataaccgat gcacgcgccc gccttaatca tttcgcgcat catcagcgtg
    16801 ttgtcggtac ggatgacggg ggtcagttca agccggtatt tcttgccgtc cgatttgcgg
    16861 gtgaggtcga gtttctgctg gttggtgtag gtcggcagga cggcaggcag ccccgccact
    16921 tcttccggtg tttccggcac gccgttgcgc ctcaggaagt cgggcgaggc aagcagggca
    16981 aattcgattt ccgccagcgg gcgcgcaatc agcgaagggg aaagggtttg ggaaacgcgc
    17041 aacgccaaat ccacgccttc ggcaatcaaa tcgacgtggc ggttgtccaa aatcagttcc
    17101 aacgccactt cgggataacg ttcgcggtat tccgccagcc agttgcatat ctggctgccg
    17161 gcaaaccaca gcggcatcgt tacgcgcagc agcccctgcg gtttttccgt ccccccggcg
    17221 gctttttgcg cggcatcgtc gagcgtgtcg agcgcgtaac tgcattgccg gtagtattct
    17281 tccccggctt cggtcaggct gaggttgcgg ctgttgtggt gcaggagttt ggcttggacg
    17341 gtgttttcca agtgactgac gtgtttgctt gccattgcgg tggagatgcc gagcgcgtcg
    17401 gcggcgcggg tgaagccgcc gctttggacg acttggcgga aaactttgag gctgaacagg
    17461 gtgtccatat tttcttgtgt ggaaaagttg tatcaataaa agcagtatat atttgaaaaa
    17521 gggaaacatc tatactctgc cgcctgaaat gaagacaaat atcaaaggag cttttatgtc
    17581 cgattgctgc aaccgtatcc aaccggtttt gctttctgtt ttgcgtatcg taaccgccta
    17641 cctgtttttg ttgcacggta cgtcgaaaat cttcgccttc cccattgaaa tgggcagcgg
    17701 ttcgcccggc gggctgttgc tgcttgccgg tattttagaa attgtcggcg gcattttgct
    17761 ggtgttgggc ctgtttgcgc gccctgccgc gtttgttttg tccggtcaga tggcggttgc
    17821 ctattttatg gcgcacgctt ccggaaatgc tttgttcccg attgccaacg gcggcgagtc
    17881 cgcagtgctg ttctgcttcg tatttctcta tatcgcggca gcgggcggcg gagcatggtc
    17941 gctggacagg ctgtttttca agcgtaaagc ctgaaccgga ctgcctaaag tgtattttgt
    18001 tgaatgtttt tgaggaaaag aaatgagccg tcaatccctg caacaggctg ccgaaagccg
    18061 ccgttccgtt tattcgttaa ataaaaacct gcccgttggc aaagatgaaa tcgtccaaat
    18121 cgtcgaacac gccgttttgc acacaccttt ttcgttcaat tcccaatctg cccgcgtggt
    18181 cgtgctgttt ggcgaagagc atgataagat actactttaa ttgacaacag catctttaca
    18241 attcattttc aacccattga atttcaatat tt
//
LOCUS       DAWTCI010000041        18238 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWTCI010000041 DAWTCI010000000
VERSION     DAWTCI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18238
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.041"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(108..1325)
                     /locus_tag="WM481_001303"
     CDS             complement(108..1325)
                     /locus_tag="WM481_001303"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFA9080612.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLATGEQVTIGLLAMALKDIGVDAKSYTGWQVSL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGNISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERKDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFDLG"
     gene            complement(1527..2255)
                     /gene="rph"
                     /locus_tag="WM481_001304"
     CDS             complement(1527..2255)
                     /gene="rph"
                     /locus_tag="WM481_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFA9080613.1"
                     /translation="MPDYIRTSRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SVDENAPPFLHGKNQGWVTAEYGMLPASTALRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPILE
                     AVAAVSAGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEGAPFSLDELG
                     KLVALAQKGIGELLRYQQNALSVA"
     gene            complement(2337..2801)
                     /locus_tag="WM481_001305"
     CDS             complement(2337..2801)
                     /locus_tag="WM481_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFA9080614.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVGNTVKHLLVANKGAREMAQDL
                     VDYADENGAGLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3018..3794
                     /locus_tag="WM481_001306"
     CDS             3018..3794
                     /locus_tag="WM481_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HFA9080615.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALTHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERITQINPQCEVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVYSTENIMPPQSGAAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4039..4893
                     /locus_tag="WM481_001307"
     CDS             4039..4893
                     /locus_tag="WM481_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFA9080616.1"
                     /translation="MPPIPALSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVEITGQYLHYIAQMEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHGIC
                     RFHDLFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG
                     TRGGRDVF"
     gene            4934..6142
                     /gene="pncB"
                     /locus_tag="WM481_001308"
     CDS             4934..6142
                     /gene="pncB"
                     /locus_tag="WM481_001308"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFA9080617.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNVSTVY
                     PLADIREDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVYLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKG
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPRTP"
     gene            6234..7952
                     /gene="argS"
                     /locus_tag="WM481_001309"
     CDS             6234..7952
                     /gene="argS"
                     /locus_tag="WM481_001309"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFA9080618.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRHEFLAQNIHAALNDA
                     RFGVAKTAQPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFTGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVADDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKAGRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENAKAEFIGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDATAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQAATLFSRFYEACPILKAEGASRNSRLQLAKLTGNTLKQGLDLLGI
                     DVLDVM"
     gene            8464..9882
                     /locus_tag="WM481_001310"
     CDS             8464..9882
                     /locus_tag="WM481_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFA9080619.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLVPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAVKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATRL
                     SEPKSQKILFIVAESWGLPGNPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQKIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIRRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
     gene            9888..10148
                     /locus_tag="WM481_001311"
     CDS             9888..10148
                     /locus_tag="WM481_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080620.1"
                     /translation="MPSERTNSLQTAFCRQTDLLKPTFFIISDKLLCKNPKIQNHRKL
                     SKKQKPSAVVPAKAGIQTRKPQKFIGKTETRKQRTEFPPAQE"
     gene            complement(10335..11081)
                     /locus_tag="WM481_001312"
     CDS             complement(10335..11081)
                     /locus_tag="WM481_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFA9080621.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAACSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTAALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(11260..11931)
                     /locus_tag="WM481_001313"
     CDS             complement(11260..11931)
                     /locus_tag="WM481_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFA9080622.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAG
                     DVGSDAPVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGE
                     WLGIW"
     gene            complement(12000..12671)
                     /gene="rpiA"
                     /locus_tag="WM481_001314"
     CDS             complement(12000..12671)
                     /gene="rpiA"
                     /locus_tag="WM481_001314"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFA9080623.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(12748..13230)
                     /locus_tag="WM481_001315"
     CDS             complement(12748..13230)
                     /locus_tag="WM481_001315"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFA9080624.1"
                     /translation="MTNIRIGQGYDVHQLIEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(13262..13951)
                     /locus_tag="WM481_001316"
     CDS             complement(13262..13951)
                     /locus_tag="WM481_001316"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFA9080625.1"
                     /translation="MKRKNIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAVPVADTLKRAESGQISATVDRSG
                     LWQAQTPQLFQAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(13948..14682)
                     /gene="dnaQ"
                     /locus_tag="WM481_001317"
     CDS             complement(13948..14682)
                     /gene="dnaQ"
                     /locus_tag="WM481_001317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFA9080626.1"
                     /translation="MMTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEEKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGETEEEEPTAKPVASAEMKLGAKLKVIKADEAELAAHE
                     EYLDGLGEACIWRKEAVPSENGGENA"
     gene            complement(14838..16148)
                     /locus_tag="WM481_001318"
     CDS             complement(14838..16148)
                     /locus_tag="WM481_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9080627.1"
                     /translation="MDAKKGGLGLVKSRRFGPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGVLPAGQMLNLGALLFILPYFLFSALSGQLGNKFDKAVLARWVKVLEIIIMAV
                     AAYGFYIRSAPLLLLCLFCMGAQSTLFGPLKYAILPDYLDDNELMMGNSLIESGTFVA
                     ILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFGRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYSVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP"
     gene            complement(16138..16323)
                     /gene="ccoS"
                     /locus_tag="WM481_001319"
     CDS             complement(16138..16323)
                     /gene="ccoS"
                     /locus_tag="WM481_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFA9080628.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKEKENDGR"
     gene            complement(16323..16622)
                     /locus_tag="WM481_001320"
     CDS             complement(16323..16622)
                     /locus_tag="WM481_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080629.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARLQSGRFAVKLEDGTGN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLIGNM"
     gene            16973..18169
                     /locus_tag="WM481_001321"
     CDS             16973..18169
                     /locus_tag="WM481_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9080630.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELEAHGLDSRIGAIGHRVVSGGELYNESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETAHFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITDMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
BASE COUNT         4251 a         5392 c         4554 g         4041 t
ORIGIN      
        1 tattttttgt tccaaagtca aaatatgccg tccgaacgtt cgggcggcag acaaaacggc
       61 actgcccgat aaaggcagtg ccgttgtccg ttttaaaccg tgaaacatca gcccaaatca
      121 aaggctttat gcaataccct ggttgccagt tccatgtatt tttcgtcaat caatacggaa
      181 actttgattt cggaggtgga aatcatttgg atgttgatgc cctcttcggc gagcgtgcgg
      241 aagattttgg cggctacgcc gacgtgcgaa cgcataccca aaccgactgc ggagactttg
      301 cacacggtgt cgtcgccgtc gatagaagct gcgccgatac tgtctttacg ttccgacagg
      361 atttccaaag tctgtttgta atcgccgcgc ggtacggtga aagaaaaatc ggttgtgcct
      421 tcgctgccga cattttggat aatcatatcg acttcgatgt tggcatctgc gaccgcgccc
      481 aaaatctgat aggcgacgcc gggcttatcg ggcacaccgc gcacgttgat gcgggcttgg
      541 tttttatcga atgcgatacc ggttacggca gctctttcca tgttgtcgtc ctcttcaaag
      601 gtaattaagg tgccgttgcc gccgtcttgc aggctgctca gtacgcgcag gcgcactttg
      661 tattttccgg cgaattctac tgaacggatt tgcaaaactt tcgaaccgag gctggccagt
      721 tcgatcattt cttcaaatgt aaccgtatcc atacggcgcg cttcgggtac gacgcggggg
      781 tcggtggtgt aaacgccgtc tacgtcggta tagatttggc attcgtccgc tttgagggct
      841 gcggcaagcg caacggcgga agtgtcggaa ccgccgcgtc ctagcgtgga aatattgcct
      901 tcactgctga tgccttggaa gccggcaacg atgacgactt tgccggcggc gaggtcggcg
      961 cgcatttttt cgtcatcaat gctttctatg cgggctttgg tgtgggcggt atcggttttg
     1021 agggagacct gccagcctgt gtagcttttg gcatccacgc cgatatcctt caatgccatt
     1081 gccaaaaggc cgatggttac ttgttcgccg gtagctaaga cgacgtccag ctcgcgcgga
     1141 tcgggatgct cctgcatttc gtgcgccaac gcaaccagac ggttggtttc gccgctcatg
     1201 gcggatacga cgacgacgat atcgtgtcct tcggcgcggg ctttagcgac acgtttggcc
     1261 acgtttttaa tgcgctcggg cgagcctacg gatgtgccgc cgtatttatg tacgattaac
     1321 gccatgtttc atgctttctt gtgtgggatt ggtcgggcag catggtttgc tggaaaagcg
     1381 gctattatta ctatttttta catgaagttc aagaacggac tgcgctttcc cgcctgccgt
     1441 ttgacagcgg tcagcgaaaa acctgttctt tcagattttt gacaaaatgc cgtctgaacg
     1501 gttttcagac ggcatacgga cagtaattaa gcaacggaca acgcattttg ctgataacgc
     1561 agcagttcgc ctatgccttt ttgcgccagt gcaaccagtt tgcccaactc gtccaaactg
     1621 aatggcgcgc cttccgccgt tccctgtatt tcgatgattt tccccgatgc ggtcatgacg
     1681 atgttcacat cactgtcgca gccggagtct tcgggataat ccaaatccaa aagcggcacg
     1741 ccgttcacta cgcccgctga tacggcggca acagcttcga ggatggggtt ttcactcaaa
     1801 atgccgtctg aaaccagttt gccgacggcg atttgcagcg cgacaaacgc accggtaatc
     1861 gaagccgtac gcgtgccgcc gtccgcctga atcacatcgc aatcaatcag gatttgtcgt
     1921 tcgccgagtt tttccatatc cacgaccgcg cgcagcgaac gcccgatcaa gcgttggatt
     1981 tcttgtgtgc gtcccgactg tttgcccgcc gaagcttcgc ggcgcatacg caaagcggtt
     2041 gaggcaggca gcataccgta ttccgccgtt acccagcctt ggtttttgcc gtgcagaaac
     2101 ggtggggcat tttcgtccac ggaagcggta caaatcactt tggtattacc gcattcaata
     2161 aggcacgaac cgtccgtatg cggcaggaaa tgaggggtga ttttgatatc gcgcaggctg
     2221 tcggcggcgc gtgaggtgcg gatataatca ggcatactgc cctcccgtta aaaacagata
     2281 aattaaaaag ccttaaatat gaaaaatcac atttaaggcc ttcaaactga aaatttctac
     2341 gcctcttcgg ctttgctacg gataatcaaa agcggcaggt ggctttggcg cattaccgtt
     2401 tcggcgaaac tgcccatcaa aaggtgcatc agcccggtgc gcccgtgcgt gcccaacacc
     2461 agcaggccgg caccgttttc atcggcataa tcgaccaaat cctgcgccat ttcgcgcgca
     2521 cccttattgg caaccagcag gtgtttgacg gtatttccca cacccagttc ccgggcggtg
     2581 cgctcggcgg catccaaaac ctcgttgcct tgcgcgacgg cggcggcctc gtaactttcg
     2641 tgttgcaaaa attcgggggc gagcgccata tattcggcag ggttggcaac gtgtaccaaa
     2701 gtcagacgcg caccgttgac cccggcaagc tcggcggcgt gtttcagggc attgatggac
     2761 gtttcactgc cgtcaacggc aacgaccaga tgtttgtaca tatcgtattc tccttttgca
     2821 ccgtctcacg gtgccgtctc ttcaggtttg cgcaaaggca gtctgcgccg tttcattata
     2881 gacccgccgt cgggctttat acaacagccg aacagcctga ctgctttcca gtataatatg
     2941 ccgctttcgt gcagtcaggc attttttgcc ggctttcgtt cactttttga tttgacgcaa
     3001 tcttgcagga ttcgaccatg tccgacaacg ctttgacctc ttcgcgacgc ttcggcggca
     3061 tcgccagact ctacggagac tctgccttga cgcacttttc acaggcacac gtctgcgtgg
     3121 tcggcgtggg cggggtcggc tcgtgggcgg tcgaggcttt ggcgcgcacg ggcatcgggc
     3181 gtttgacttt gattgatttg gacaacgttg ccgaatcgaa tgtcaaccgc cagctgcacg
     3241 ccctgaccgg cgacttcggc aaagcaaaag ttaccgcctt gcgcgaacgt attacacaaa
     3301 tcaatccgca atgcgaagta tttgaaattg aagatttcgt taccgaagac aatttgccgg
     3361 aatacttcgg aaaaggtttt gatttcgtca tcgacgcgat cgaccaagtg cgcgtcaaag
     3421 cggcaatggc ggcttatttt gtggaacgca aacaaccgtt tgtcctcagc ggcggcgcgg
     3481 gcggacagaa aaatcccgcc ctgattcaaa ccgccgattt aagccgcgta acccacgacc
     3541 cgctgcttgc caacctgcgc tacaccttgc ggaaacgcta cggcttcagc cgcgatacta
     3601 aggaaaaaat gcgcgtgccg tgcgtgtatt cgaccgaaaa tatcatgccg ccgcaatcgg
     3661 gcgcagcgtg ttctgccgat gccgcgccgc agggcttgtc atgcgccggc tatggcgcaa
     3721 gtatgctcgt taccgcttcg ttcggactgt attgcgcaca ggcggcggtg gaacacatcg
     3781 cgggcaaaaa ataagcaatg ccgtctgaaa caggattcag acggcatttg aacaaacgcg
     3841 gcaatcaagc tgtcggttca gaaaaaccaa catcggcacg cctaaaaccg tattcacaat
     3901 ctactccttt tcaaaatatt tgcatttaaa agccgttata atgccgtccg aacatctgcc
     3961 cgaccacatt atgcgtgaat gccggcagat tgttttcttt tgtaaactta tattaaaatt
     4021 cacttaccaa ttcacgccat gccgcccatc cctgccctat ccgcaccatc cgagcacact
     4081 gtcgcatggg tattcggcca acccgttacc gatttgccgc aggatttgtt tatcccgccc
     4141 gacgcgctga aagtcgtatt gggcagtttt caaggccctt tggatctgct gctctacctg
     4201 attcgcaaac agaatatcga cgtactggat attccgatgg tggaaattac cgggcagtat
     4261 ctgcactata ttgcccaaat ggaagcctat cagtttgatt tggcggcgga atatcttttg
     4321 atggcggcaa tgctgattga aatcaaatcg cgcctgctgc tgccgcgtac cgaagccgtc
     4381 gaagacgaag aggccgaccc gcgtgccgag ttggtgcgcc gtctgcttgc ctacgagcaa
     4441 atgaaactgg cggcgcaggg tttggacgcg ctgccgcgtg cgggacggga tttcgcgtgg
     4501 gcttacctgc cgctggaaat tgcagccgag acgaagctgc ccgaggttta catcgccgat
     4561 ttgatgcagg catggttggg cattctttct cgggcaaaac atacgcgcag ccacgaagta
     4621 atccaagaaa ccctttccgt gcgcgcgcaa atgacggcaa tcctgcgccg tttgaacgaa
     4681 cacgggatat gcaggtttca cgacctgttc aatcccgaac agggcgcggc ttacgtgatc
     4741 gtcaacttca tcgccctgtt ggagcttgcc aaagaaggat tggtcggaat cgtacaggaa
     4801 gacggtttcg gagaaatccg aatcagcctc aatcatgagg gggcgcattc agacggcatt
     4861 ttcggcacac ggggcgggcg cgatgtgttc taatacgccc caagccgcca ccagaaatcg
     4921 ggagacacgc catatgaccg gcatcataca ttcgctgctc gacactgacc tctacaaatt
     4981 cactatgctg caagtagtcc tgcaccagtt tccgcagacg cacagccttt acgaattccg
     5041 ctgccgcaac gtctcgaccg tctatccgct tgccgacatc agggaggact tggaagccga
     5101 actcgacgcg ctctgccggc tgcgcttcac ccacgacgaa ctcggctatc tgcgctccct
     5161 gcgtttcatc aaaagcgact ttgtcgatta tctcgaactc ttccagctcc aacgccgctt
     5221 tgtcgaagtc ggcacagacg ataaaggccg tctgaacatc cgtatcgaag gcccgatgat
     5281 acaggcgatg ttttttgaaa tcttcatcct cgccattgtc aacgaacttt acttccgccg
     5341 gctggaaacc cctgccgtta tcgaagaagg cgaacgccgg cttcaagcca aagccgcgcg
     5401 cctgaaagaa atcgccgccg cacagaatcc ggacgaaccg cccttcctga tttccgactt
     5461 cggcacgcgc cgccgctaca agctcgcgtg gcaggaacac gtcatccgca ccctgctcga
     5521 agccgcccca agcatcgtgc gcggcacaag caacgtttac ctcgccaaaa aactcggcat
     5581 cacccccatc ggcaccatgg cgcacgaatt cctccaagcc ttccaagccc tcgacgtgcg
     5641 cctgcgtaat ttccaaaagg ccgcgctcga aagctgggtg cacgaatacc ggggcgattt
     5701 gggcgtcgcc ctgaccgacg ttgtcggtat ggatgccttc ctgcgcgatt tcgacctcta
     5761 tttcgccaaa ctcttcgacg ggcttcgcca cgacagcggc gacccttacg tttggggcga
     5821 caaagcctac gcccactatc aaaagctcaa aatcgacagc cgcaccaaaa tgctgacctt
     5881 ctccgacggg ctggacatcg aacgctcttg ggcattgcac caatatttca aaggccgctt
     5941 caaaaccggc ttcggcatcg gcaccaacct caccaacgat atggggcata cgcccttgaa
     6001 tatcgtcttg aaactggtcg aatgcaacgg gcagtccgtc gccaagctct ccgactcgcc
     6061 gggcaaaacc atgaccaaca acagcacctt cctcgcctac ctgcgccaag tgttcgacgt
     6121 acccgaacct agaacgccgt aaaccggcag aaaaagcgta caattcctgt ttccgccgca
     6181 taaaatcttt taaaataccg cctgatttga atttaaccga aagaccgaac ttcatgaacc
     6241 tacatcaaac cgtcgaacac gaagccgccg ccgcctttgc cgccgcaggc atcgccggca
     6301 gccccgttgt cttgcagccg accaaaaatg ccgaacacgg cgatttccaa atcaacggcg
     6361 tgatgggtgc ggcgaaaaaa gccaaacaaa atccgcgcga actggcgcaa aaggtcgccg
     6421 acgcattggc gggcaacgcc gtcatcgaaa gcgcggaagt ggccggaccc ggctttatca
     6481 acctgcgcct gcgccacgaa tttctcgctc aaaacattca tgcggctttg aacgacgcgc
     6541 gtttcggcgt agcgaaaact gcccaaccgc aaaccgtcgt tatcgactat tcttcgccta
     6601 atctggcgaa ggaaatgcac gtcggtcatc tgcgctccag catcattggc gacagcattt
     6661 cgcgcgtgtt ggaatttacg ggcaacaccg tcatccgcca aaaccatgtc ggcgactggg
     6721 gtacgcagtt cggcatgttg gttgcttatt tggtcgagca gcagaaagac aatgccgcgt
     6781 tcgagctggc ggatttggag cagttttacc gcgccgccaa agtgcgcttt gacgaagacc
     6841 ctgcctttgc cgacaccgcg cgcgaatacg ttgtgaagct gcaaggcggc gatgaaaccg
     6901 tgttggcgtt gtggaaacag tttgtcgata tttcgctctc gcacgcccaa gccgtttacg
     6961 acacgctggg cttgaagctg cgccccgaag atgtggcggg cgaatcgaaa tacaacgacg
     7021 atttgcagcc tgtagccgat gatttggttc aaaaaggcct ggcggttgag gacgacggcg
     7081 cgaaagtcgt gttcttggac gagtttaaaa acaaagaagg cgaacccgcc gcatttatcg
     7141 tgcaaaaaca aggcggcggc ttcctttatg cctccaccga tttggcgtgc ctgcgctacc
     7201 gcataggccg tctgaaagcc ggccgcctgc tgtacgtcgt cgaccaccgc caagccctgc
     7261 acttcgagca actctttacc acttcccgca aagcaggcta tctgcctgaa aatgcaaaag
     7321 ccgagtttat cggcttcggc accatgatgg gcaaagacgg caaaccgttc aaaacgcgca
     7381 gcggcgacac cgtaaaactg gttgacctgc tgaccgaagc cgtcgagcgc gccaccgctt
     7441 tggtgaaaga aaaaaatccc gaattgggcg cggatgaagc cgccaaaatc ggcaagaccg
     7501 tcggcatcgg cgcagtcaaa tacgccgatt taagcaaaaa ccgcaccagc gattatgtgt
     7561 tcgactggga cgccatgctc tcatttgaag gcaacaccgc cccctacctg caatacgcct
     7621 acacccgcgt gcaaagcgtg ttccgcaaag ccggagaatg ggacgcaacc gcaccgaccg
     7681 ttttgaccga accactggaa aaacagcttg ccgccgagct tctgaaattt gaaaacgtgc
     7741 tgcaaagcgt ggcggacacc gcgtatccgc actacctcgc cgcctacctt taccaagccg
     7801 ccaccctgtt cagccgcttc tacgaagctt gcccgatttt gaaagccgaa ggcgcaagcc
     7861 gcaacagccg cctgcaactg gcaaaactca ccggcaacac gctgaaacaa ggcttggatt
     7921 tgctgggcat cgatgtgttg gacgtaatgt aaacaccgca ccgcccgcca tcctcatccg
     7981 aatctgaaaa aagcggcgcg atacaccgta tccgccgcct ttcccaaaat gcgaaacaaa
     8041 caaacgccaa gcaagcaagc aagcaagcaa aaattatagc ccctttcctg ccggcgcaca
     8101 cactttccgc gcggcacatc cccctttttc cgcccctcaa acccgccttt tcttcaggca
     8161 gggtttcagc ccgcctcctt tccctgtttt cctttccccg acacgcgtgc gcgcccctgc
     8221 cgcaccgtgc cgcgcttttg cgcccggacg gcatcgttcc gccatctggt tttctgtttt
     8281 aaataccgct gtttcagaaa gaaatgcaga tgtttcgaca cacaggacga catataaagt
     8341 accgccctat gtgtcgtcct gatttggaag gggttacacc cctcccaacc agataaagtc
     8401 tgatcctgcc gccccgaagg acggatgtcc aagtggcggg gtttcaaccg aaaaggaaat
     8461 acgatgaata ttcacgccct gctctccaaa caatggacgc tgccgccatt cctgccgaaa
     8521 cggctgctgc tgtccctgct gatactgctg gtccccaatg cggtgttttg ggttttggca
     8581 ctgctgaccg ccaccgcccg cccgattgtc aatttggact accttcccgc cgcgctgctg
     8641 atcgccctgc cttggcgttt cgtcaaaatt gccggcgtat tggcgttttg gctggcggtt
     8701 ttgtttgacg ggctgatgat ggtgatccaa ctcttccctt ttatggacct catcggcgcc
     8761 atcaacctcg tccccttcat cctgaccgcc cccgcccctt atcagataat gaccgggctg
     8821 ttgctgctgt atatgctggc gatgccgttt gtgttgcaaa aagccgccgt caaaaccgac
     8881 ttccgacaca ttgccgtctg tgccgccgtt gtggcggcag ccggctattt caccggccat
     8941 ttgagttact acgaccgggg gcggatggcc aatatcttcg gcgcaaacaa cttctattac
     9001 gccaaaagtc aggcgatgct ctacaccgtc agccagaatg ccgactttat taccgccggc
     9061 ctggtcgacc ccgtcttcct ccccttgggc aatcagcagc gtgccgccac gcggctgagt
     9121 gagccgaaat ctcaaaaaat cctctttatc gtcgccgaat cttgggggct gccgggcaat
     9181 cccgagcttc aaaacgccac ttttgccaaa ctgctggcgc aaaaagaccg tttttcggtt
     9241 tgggaaagcg gcagttttcc cttcatcggc gcgacggtcg aaggcgaaat gcgcgaattg
     9301 tgcgcctacg gcggtttgcg cgggttcgca ctgcgccgcg cacccgacga aaaatttgcc
     9361 cgctgcctcc ccaaccgttt gaaacaagaa ggttacgcca cctttgcgat gcacggcgcg
     9421 ggtagttcgc tttacgaccg cttcagctgg tatccgaggg cgggctttca aaaaatcaaa
     9481 accgccgaaa acctgatcgg taaaaaaacc tgcgccattt tcggcggcgt gtgcgacagc
     9541 gagctgttcg gcgaagtgtc ggcatttttc aaaaaacacg acaagggact gttttactgg
     9601 atgacgctga ccagccacgc cgactatccc gaatccgaca ttttcaacca caggctcaaa
     9661 tgcaccgaat acggcctgcc cgccgaaacc gacctctgcc gcaatttcag cctgcacacc
     9721 caattcttcg accaactggc ggatttgatc cgacgccccg aaatgaaagg cacggaagtc
     9781 atcatcgtcg gcgaccatcc gccgcccgtc ggcaacctca atgaaacctt ccgctacctc
     9841 aaacagggac acgtcgcctg gctgcacttc aaaatcaaat aacaacaatg ccgtctgaac
     9901 gcaccaacag ccttcagacg gcattttgca gacagaccga cctcctcaag cccacttttt
     9961 tcatcatctc cgataaattg ctttgtaaga atccaaaaat ccaaaatcac aggaagttat
    10021 caaaaaaaca gaaaccatcc gccgtcgttc ccgcgaaagc gggaatccag acccggaaac
    10081 cgcagaaatt tatcggaaaa accgaaactc gaaaacaacg gacagaattc ccgcctgcgc
    10141 aggaatgacg gctcatacat cacccaaaaa aaattgaaac caaacagctg ggattccctc
    10201 ttgcgtgagg ctgacagatg ccgtctgaaa gactttcaga cggcataggg cgttttctct
    10261 ttgaatttaa attttgccac aaaaaagccg ccccaaatga atacccgggc agctttttgt
    10321 tgatatgact ccaatcagcg gtgttgcgga ttgtaacgtt tttccaaacg caggaatatc
    10381 cagcctaaga aagtcgtcat caacagataa atcagggcgg cggtgtaaag cggttcttca
    10441 taaaccgaat accggcccgt aatcgtattc tgaacatacg ccaactccgc cacagcaatg
    10501 accgacagca gcgagctgtc tttcaagagc gtgatgaact cgctcgccaa aggcggcagc
    10561 atacggcgca atgcctgcgg cagaatcaca tagcgcatcg cctgcggata ggtcagtccc
    10621 aaagaacacg ccgcctccat ctgtcctttg tctatagact ggatgcccgc gcggaaaatc
    10681 tcacagatat acgcccccga gttggcgatc agtgccaaag aaccggcaat cagcggcccg
    10741 tatccgcgac gcagcgcgat tgccgcctcg ccgctgacca aaatgccgtc tgaaggatgg
    10801 acgaaaaacg gaaaccacac atacgcccaa atcacaatct gcacaaacag cggcgtaccc
    10861 cggaacaggg taacgtacag cagcgaaacc ttacgcaacg cccacgccag cacgcgcatc
    10921 ggcgcaccgg ctttttccaa gtgaatcagg cgcgccaacg ccaacaacag acccaatacc
    10981 gaaccgcccg ccgtcgccac gaccgtcagc cccaaggtcg tcagtgcgcc gtaaagaaac
    11041 atccagcggt attcgtaaat aatgtcaaaa cgaaaatcca taaaccgtcc gtatcaaaaa
    11101 ccggcggaac tgccgccgtt gcaaaataat ccgccatttt accgtaaaaa ccgccgcctg
    11161 aactttttta tcgcggcaga cggcggttgc gcgtctccgc aaaaatgcag ggcgcgcggt
    11221 tttcagacgg catttgccgt tcaaggccgt gcggtgtctt taccaaatgc ccaaccattc
    11281 gcccacggaa tccatccaat ccttattgcc cccgccgctc ctgcctgccc ggcggtacgc
    11341 ccacggcgct tgcggatttt tagctttcca caatcctttg cgttcccttt ccgcctgaat
    11401 ttgagcgtcg gcatagtcgg caaaatccgc cttatcctgc tgttctttag cataactttt
    11461 ataatgccac gccgccccgt cctgcacctg catcaggttc aaatcggttt tgccggcgga
    11521 tacctgcgcc acttcgcgct gatagcggtc ggtttcaaac acacgtacac tgactttcct
    11581 accctccgcc gccgcgcgca ggttgtcgcg cgaacgtgta ccgtaagcct gtttcatctc
    11641 cggtgcgtcg atatacgcca tccgaatttt atgtttcgcg ccgtcgccgt cgatgacgtg
    11701 aagggtatcg ccgtcataga ctttggacac cgtgcctgtg tagctgtggc cggatttcgc
    11761 cgatgcccgt cggcgaacgg gcgcgtcgga acccacgtcc cctgccgtgc cgagtacgtc
    11821 gagtacggca accgccgtcc gcaccgcctc actgtcatat cccgtataac ccaacgcgcc
    11881 caaaagcgac agggcgacgg gaagccattt catgattttt ttaatctgca tatttttcaa
    11941 atgccgatgc cgtctgaaca tctctgaacc ggatttcaga cggcatctta acgtcaggat
    12001 tacccttggc agggatagat gactttcgca ccctcttccg tccccaaaat caacacatcg
    12061 gcggcatcgc gggcgaatat gccgttttcg agtacgccgg tgattttgtt gatttcatct
    12121 tccatcgtca gcggctgatc gatattcaag ccgtggacat cgacgatttg gttgccgtaa
    12181 aacgtggtgt agccgatacg cagttcgggc tgtccgccca tggcaagcag tttgcgcgaa
    12241 acaagggagc gcgcgctttc gacggcttcc acgggcagtg ggaatttgcc cagccgggaa
    12301 acatatttgc tttcgtccgc aatgcagacg aatttttctg atgcgctggc gacgattttt
    12361 tcgttgaggt gcgcgccgcc gccgccctta atcatttgca gggcgtggtt cacttcgtcc
    12421 gcaccgtcga tatagaccgc caaaccggac acttcgttca gggacacgac gggaatgtcg
    12481 taccgggcaa gcagttcgcc ggattttttg gaggttgaaa ccgcgccttt gattttttta
    12541 ccgcttttgc ccaaggcttc gatgaagaaa ttgatggtcg aaccggtacc gatgccgatg
    12601 tattcgtttt cgggtacgaa ttcgactgct ttttcggcgg cgatgcgctt gagttcgtct
    12661 tgtgtcgtca tatttttatc ctttgggaaa ccgtatcaac aaacagccgc catcttaaca
    12721 tttttttgca cgtcctgccc gccgcgttca aatccgtacc agcaataccg ccgcctgcgc
    12781 ctctatgcct tccatccgcc cgagatagcc gagtttttcg ttggttttgc ctttgatatt
    12841 gacgcacgaa atatcgatac ccaaatcggc ggcgatgttg gcacgcattt gcggaatgtg
    12901 cggtgcgagt ttgggtttct gtgcaatcac ggtcgtatcg acattgaccg cctgccaacc
    12961 ctgcgcctga acgctttgat acgccgcacg caaaaggacg cggctgtccg catctttgaa
    13021 ctctgcggcg gtgtcgggaa aatggctgcc gatgtcgccc aaacctgccg caccgagcag
    13081 cgcgtcggta acggcgtgca gcagcgcgtc ggcatcggaa tgcccgagca gccctttttc
    13141 aaatgggatt tcaactccgc caagtatcag ctttctgcct tcgatcaatt ggtggacatc
    13201 gtagccctgt ccgatacgga tattcgtcat cgtttgtgtt cctgatgttt tgaattgaat
    13261 ttcagacggc attgagcagc agcctgacga tgtatgcgtc ctgcggctgc gtcagtttca
    13321 aattgcgcgc gtcgccctgt atcagtagcg gacgcacacc cagtttttcc acggcggacg
    13381 cttcatcggt aatgccgtcc aagttttccg cagccaatgc gcggtgcagc aaacccgctt
    13441 gaaaaagctg cggcgtttgc gcctgccaaa gccccgaacg gtcgacagtt gcactgattt
    13501 gtccgctttc tgcgcgcttg agcgtatcgg caacgggaac tgccaaaatc ccgccttcgg
    13561 cggcgttgcc cgcctgttct atcaaccgcg tcagagcttc agacggcagg cagcagcggg
    13621 cggcatcgtg taccagaata ttgtcggttt ccgccgccaa accggtttcc aacagttttg
    13681 ccacaccgtt gcggacagtt tcggcgcggg tctgtccacc gtttttccac acccgaacct
    13741 gtggaaatgc cgtctgaacc ttatcggcaa acgtgtcttc gggcgagacg acaacgacgg
    13801 tcaaatcgac ggcctcatgc cgttcaaaaa tcccaagtac atgttctaaa acggttttgc
    13861 ttccgatttc gacatattgc ttgggtttgt ccgcaccgaa acgcaccccg atgccggcgg
    13921 cgggaatcag cgcgatattt ttgcgtttca tgcgttttcc ccgccgtttt cagacggcac
    13981 ggcttcctta cgccaaatac aggcttcgcc caagccgtca agatattctt cgtgcgccgc
    14041 cagttcggct tcatccgcct taatcacttt cagtttggca ccgagtttca tttcggcaga
    14101 tgcaacaggt ttggctgtcg gttcttcctc ctcggtctcc cccatcaagt cgaattgccg
    14161 ccgcgtcatc gccagataga cttcgcccaa aagttcgcag tcgatcaatg cgccgtgcag
    14221 gacgcgcttg ctgcggtcga cggaaaaacg gttgcacagg gcatccagac tggctttctg
    14281 cccggggaac atttcgcgcg ccatcgccag ggtatcggta acggtgcagc cgagttcctc
    14341 gacggtcggc agccccatcc ggcggaactc catattgagg aagcccacat cgaatttggc
    14401 attgtggata atcagttccg caccgcgcag aaaatcggca atctgcctgc cgacctctgc
    14461 aaacggcggc gcgtttttct cttccaaaac ctgtatcgtc aagccgtgga cgcgtgccgc
    14521 ctcttcgggc atatcgcgct cggggtggac atagaggtgc aggtttttgt cggtcatttg
    14581 gcggttgacc atttccagac cggcaaactc gaccagacgg tcgccgccgt cggcatacag
    14641 gccggtggtt tcggtatcga ggatgatttg gcgtgtcatc atatcggtgt ctttcttctg
    14701 tcttcgtaaa ttgattattt tttaagcaat gtatttttct gttttcattt caatgcacaa
    14761 acccacttat tcacagtgtg ttcacaacat tgggcaggcg gattgtgtat tttggggaca
    14821 attttttcag acggcattca aggttttttc ctgattgccg ccgcgcctaa aaaccgcctt
    14881 tcgcgcttaa tcaaaaatac cgccaacgga atattgccca aggcgacaat cagatacagc
    14941 agggaaatgc tgtcaaacaa aaacagcaat accgcgctca aaacggcggc ggaaaccata
    15001 aagatgccgt taacgatatt gttggcggca acggcgcggg cgcggaaagt ctcgctgctg
    15061 gcggtttgca gccaggtata gagcggaacg gagaaaaatc cgccgaaaaa gccgatcagc
    15121 gtcatcaccg ccatcacgga gtatgcccat ccttgcgata aaaaccaaaa aatgccgttc
    15181 agcccttcaa aacggtgtcc gtgcgtcagc cacaccaaaa ccaggccgca aaccgtcaaa
    15241 cccaacgcac caaccgttac ccaagccaac atcagccgtt ccctgccgaa cttggcacac
    15301 agtaccgaac cggcggcaat accgatggaa aacaaagcaa gcatcaggtt aaaaacatta
    15361 tcgttgccgc ccaaatggat ttgggtaaag gtcggcagtt gcgtggtata aaccgcgccg
    15421 acaaaccaaa accacgagat gccgataatg gcggtaaaaa cgggattgtg ccgcaccgtt
    15481 tcacgcagca gggattttgt accacggaca atattccact cgatttgggt atcggcagcc
    15541 ttggcgggta cggacggcat aaacaggctg ccggccgttc ctccgacggc gaccagcaaa
    15601 accagtatcc cgacaatata aggcggcgcg ccggcaaccg ccgttcccaa aatctgacca
    15661 aacaggatgg cgacaaacgt accggattca atcaggctgt tgcccatcat cagctcgttg
    15721 tcgtcgagat aatcgggcag aatggcatat ttaagcgggc cgaacagcgt cgattgcgcg
    15781 cccatgcaaa acaggcagag caagagcagc ggggctgacc ggatataaaa cccgtatgcc
    15841 gccaccgcca taatgatgat ttccagcacc ttgacccaac gcgccaaaac ggccttgtcg
    15901 aatttgttac ccaactgccc cgacagcgcg gaaaacagga aatacggcaa aataaacagc
    15961 agtgcgccca agttcaacat ctgtccggca ggcagaacgc cgttttgccc caaaccgtaa
    16021 aacccgatca tcacaaacag cgcggttttg aacacattgt cattgaacgc gccgagaaac
    16081 tgcgtagcga aaagaggccc gaaacggcgg cttttaacca gtcccaaacc gcctttttta
    16141 gcgtccatcg ttttccttct ctttttcaat cagtttactt gtcgaatcat catccatcaa
    16201 aatgcggtgc gccggtcctt ccaaatcgtc aaactgtccg tttttgcccg accaccagaa
    16261 aaaccagccg atgacaaatg ccaaaataat gctgatgggc accagaataa acatactttc
    16321 catcacatat tccctatcaa atcgttcaaa acaaaagtct gccctgacac ggtcagatat
    16381 tcgttacgca aagttccgac gggggcttcg tcaaaaaaca gctcgatacg gtctttgacc
    16441 acgcgccaat attggggaat ttccgtctga ccgaacggcg acaggacatg gttgcccgtc
    16501 ccatcttcca atttaacggc aaaacgcccg ctctgcagcc gtgcttccga ttcgtcgtcg
    16561 gcaagaagga tgaaaaagcc tatatgccgt cccgattggt catgaatact gaaataatgc
    16621 ataaattccc cacccgcctt ttttcagacg gcatcaacta aaaacagggc gaatgtacca
    16681 gtttggacgg gaaaaatgca aagaaattct ccctccccca gccgaaaaca ccggcaaacc
    16741 gcatatcccc cttttttccg tcaaaatacc cgacttccgc cattttcacg caaacgcccg
    16801 attaagccaa gcaattgcaa agattttttg ctagaatagc ctgcttcttt tatcaaccgt
    16861 ttcagacggc tccactactt tcccgtcaag gaaagcaaag cggattcggc acgaatccga
    16921 ttagtatccg tgtccgattc caatgccgtc tgaaactttc cggagtaaga aaatgtccca
    16981 aaaattgatc ttggttttga actgcggcag ctcgtccctc aaaggcgcgg tcctggataa
    17041 cggcagcggc gaagtcctgc tgagctgcct tgccgaaaaa ctcaacctgc ccgatgccta
    17101 cattacattc aaagtaaacg gcgaaaaaca caaagtcgat ctgtccgcac accccgacca
    17161 caccggcgcg gtcgaagccc tgatggaaga actcgaagcc cacggcctcg acagccgcat
    17221 cggcgccatc ggccaccgcg tcgtcagcgg cggcgaactg tacaacgaat ccatcctcgt
    17281 tgacgacgaa gtcatcgccg gcatcgaaaa atgcatcccg ctggctcctc tgcacaatcc
    17341 cgcccacctc ttgggcctgc gtgccgcgca aagcattttc aaaggcctgc ccaacgtcgt
    17401 cgtattcgat acctccttcc accaaaccat gcccgaagtc gcctacaaat acgccgttcc
    17461 gcaggagttg tatgaaaaat acggcctgcg ccgctacggc gcgcacggta ccagctaccg
    17521 cttcgttgcc gacgaaaccg cgcacttcct cggcaaagac aaaaaagacc tgcgtatggt
    17581 cattgcccac ttgggcaacg gcgcatccat caccgccgtc gccaacggag aatcgcgcga
    17641 caccagtatg ggcctgaccc cgctggaagg gctggtaatg ggtacgcgca gcggcgacat
    17701 cgatccttcc gtattcggct tcctcgccga aaacgccaat atgaccattg cccaaatcac
    17761 cgacatgctg aacaaaaaat ccggcctgct cggcatttcc ggcctgtcca acgactgccg
    17821 caccattgaa gaagaagccg ccaaggggca taaaggcgcg aaattggcct tggatatgtt
    17881 tatctaccgc cttgccaaat acatcggcag tatggcggtt gccgcaggcg gcttggacgc
    17941 actggtcttt accggcggta tcggcgaaaa ctccgacatc atccgcgaac gcgtaatcgg
    18001 ctacttgggc ttcctcggtc tgaacatcga ccaagaagcc aacctgaaag cccgcttcgg
    18061 caacgccggc gtgattacca ctgccgacag caaagccgtt gccgtggtca ttccgaccaa
    18121 cgaagagctg atgattgccc acgataccgc ccgtttgagc ggtctgtaag gttttatcct
    18181 cacgcgaact gcctccggaa acggaggcag tttttttata gtggattaaa tttaaacc
//
LOCUS       DAWTCI010000042        17771 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWTCI010000042 DAWTCI010000000
VERSION     DAWTCI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17771
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.042"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            17..328
                     /gene="rpsJ"
                     /locus_tag="WM481_001322"
     CDS             17..328
                     /gene="rpsJ"
                     /locus_tag="WM481_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFA9080631.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(414..596)
                     /locus_tag="WM481_001323"
     CDS             complement(414..596)
                     /locus_tag="WM481_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080632.1"
                     /translation="MDASAQTKPDSTFFRGASLRKVMDKKYPVLFTKKCRLKILEAQN
                     KPISPTALFFDFVVLP"
     gene            complement(670..783)
                     /locus_tag="WM481_001324"
                     /pseudo
     CDS             complement(670..783)
                     /locus_tag="WM481_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1114..1758
                     /gene="rplC"
                     /locus_tag="WM481_001325"
     CDS             1114..1758
                     /gene="rplC"
                     /locus_tag="WM481_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFA9080633.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDAD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDVERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            1758..2378
                     /gene="rplD"
                     /locus_tag="WM481_001326"
     CDS             1758..2378
                     /gene="rplD"
                     /locus_tag="WM481_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFA9080634.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2375..2689
                     /gene="rplW"
                     /locus_tag="WM481_001327"
     CDS             2375..2689
                     /gene="rplW"
                     /locus_tag="WM481_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFA9080635.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            2695..3528
                     /gene="rplB"
                     /locus_tag="WM481_001328"
     CDS             2695..3528
                     /gene="rplB"
                     /locus_tag="WM481_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFA9080636.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     HNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            3534..3812
                     /gene="rpsS"
                     /locus_tag="WM481_001329"
     CDS             3534..3812
                     /gene="rpsS"
                     /locus_tag="WM481_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFA9080637.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAVRASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGYKLGEFSLTRTFKGHLADKKAKKK"
     gene            3821..4150
                     /gene="rplV"
                     /locus_tag="WM481_001330"
     CDS             3821..4150
                     /gene="rplV"
                     /locus_tag="WM481_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFA9080638.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4160..4852
                     /gene="rpsC"
                     /locus_tag="WM481_001331"
     CDS             4160..4852
                     /gene="rpsC"
                     /locus_tag="WM481_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFA9080639.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVIIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPIHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4836..5252
                     /gene="rplP"
                     /locus_tag="WM481_001332"
     CDS             4836..5252
                     /gene="rplP"
                     /locus_tag="WM481_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFA9080640.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5252..5443
                     /gene="rpmC"
                     /locus_tag="WM481_001333"
     CDS             5252..5443
                     /gene="rpmC"
                     /locus_tag="WM481_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFA9080641.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5443..5706
                     /gene="rpsQ"
                     /locus_tag="WM481_001334"
     CDS             5443..5706
                     /gene="rpsQ"
                     /locus_tag="WM481_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFA9080642.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHSLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5928..6296
                     /gene="rplN"
                     /locus_tag="WM481_001335"
     CDS             5928..6296
                     /gene="rplN"
                     /locus_tag="WM481_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFA9080643.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAVPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6308..6631
                     /gene="rplX"
                     /locus_tag="WM481_001336"
     CDS             6308..6631
                     /gene="rplX"
                     /locus_tag="WM481_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFA9080644.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGGKVVVEGVNVVKRHQ
                     KPNPMRGIKGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            6641..7180
                     /gene="rplE"
                     /locus_tag="WM481_001337"
     CDS             6641..7180
                     /gene="rplE"
                     /locus_tag="WM481_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFA9080645.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLERIAGQRPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7183..7488
                     /gene="rpsN"
                     /locus_tag="WM481_001338"
     CDS             7183..7488
                     /gene="rpsN"
                     /locus_tag="WM481_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFA9080646.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            7504..7896
                     /gene="rpsH"
                     /locus_tag="WM481_001339"
     CDS             7504..7896
                     /gene="rpsH"
                     /locus_tag="WM481_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFA9080647.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7910..8443
                     /gene="rplF"
                     /locus_tag="WM481_001340"
     CDS             7910..8443
                     /gene="rplF"
                     /locus_tag="WM481_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFA9080648.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKKLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVASEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8457..8810
                     /gene="rplR"
                     /locus_tag="WM481_001341"
     CDS             8457..8810
                     /gene="rplR"
                     /locus_tag="WM481_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFA9080649.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8829..9347
                     /gene="rpsE"
                     /locus_tag="WM481_001342"
     CDS             8829..9347
                     /gene="rpsE"
                     /locus_tag="WM481_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFA9080650.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9340..9525
                     /gene="rpmD"
                     /locus_tag="WM481_001343"
     CDS             9340..9525
                     /gene="rpmD"
                     /locus_tag="WM481_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFA9080651.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTS
                     ENRGMINKISYLLKVES"
     gene            9527..9961
                     /gene="rplO"
                     /locus_tag="WM481_001344"
     CDS             9527..9961
                     /gene="rplO"
                     /locus_tag="WM481_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFA9080652.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTVSANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEDVGGKIEM"
     gene            9973..11283
                     /gene="secY"
                     /locus_tag="WM481_001345"
     CDS             9973..11283
                     /gene="secY"
                     /locus_tag="WM481_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFA9080653.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALLIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRVGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            11288..11506
                     /gene="infA"
                     /locus_tag="WM481_001346"
     CDS             11288..11506
                     /gene="infA"
                     /locus_tag="WM481_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFA9080654.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            11527..11640
                     /gene="rpmJ"
                     /locus_tag="WM481_001347"
     CDS             11527..11640
                     /gene="rpmJ"
                     /locus_tag="WM481_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFA9080655.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDPRHKQRQG"
     gene            11706..12068
                     /gene="rpsM"
                     /locus_tag="WM481_001348"
     CDS             11706..12068
                     /gene="rpsM"
                     /locus_tag="WM481_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFA9080656.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12088..12483
                     /gene="rpsK"
                     /locus_tag="WM481_001349"
     CDS             12088..12483
                     /gene="rpsK"
                     /locus_tag="WM481_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFA9080657.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            12503..13123
                     /gene="rpsD"
                     /locus_tag="WM481_001350"
     CDS             12503..13123
                     /gene="rpsD"
                     /locus_tag="WM481_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFA9080658.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13149..14135
                     /gene="rpoA"
                     /locus_tag="WM481_001351"
     CDS             13149..14135
                     /gene="rpoA"
                     /locus_tag="WM481_001351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFA9080659.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVGGVQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKSPPIDPVLLHPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14159..14527
                     /gene="rplQ"
                     /locus_tag="WM481_001352"
     CDS             14159..14527
                     /gene="rplQ"
                     /locus_tag="WM481_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFA9080660.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLASRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            14674..15387
                     /gene="minC"
                     /locus_tag="WM481_001353"
     CDS             14674..15387
                     /gene="minC"
                     /locus_tag="WM481_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFA9080661.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAVAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVNQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15416..16231
                     /gene="minD"
                     /locus_tag="WM481_001354"
     CDS             15416..16231
                     /gene="minD"
                     /locus_tag="WM481_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFA9080662.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSRKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILRIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16235..16498
                     /gene="minE"
                     /locus_tag="WM481_001355"
     CDS             16235..16498
                     /gene="minE"
                     /locus_tag="WM481_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFA9080663.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQTPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            16502..17422
                     /gene="oxyR"
                     /locus_tag="WM481_001356"
     CDS             16502..17422
                     /gene="oxyR"
                     /locus_tag="WM481_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFA9080664.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLKEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIHD"
BASE COUNT         4896 a         3340 c         4385 g         5150 t
ORIGIN      
        1 tgaaggatat tgataaatgg caaaccaaaa aatccgtatc cgcctgaaag cttatgatta
       61 cgccctgatt gaccgttctg cacaagaaat cgttgaaact gcaaaacgta ccggtgctgt
      121 tgtaaaaggc ccgattcctt tgccgaccaa aatcgagcgt ttcaacattt tgcgttctcc
      181 gcacatgaac aaaacttccc gtgaacaatt ggaaatccgc acccatttgc gcctgatgga
      241 catcgtggat tggaccgata aaactaccga tgcgctgatg aagctggatt tgccggccgg
      301 tgttgatgta gaaatcaaag tccaataatt cggactgtcg aaaatcccca agcaatcaat
      361 gcttggggat tttttatgtt atgccgtctg aaaatatgcg gcggcaaaca gatttaaggc
      421 aaaacaacaa aatcaaaaaa cagtgcggtc ggtgaaatcg gcttattttg tgcctcaaga
      481 attttcagac ggcatttttt agtgaacaaa acaggatatt ttttatccat aactttccta
      541 aggcttgccc cccgaaaaaa ggtagaatcg ggcttggttt gggcagaagc atccatctga
      601 ttctaaggat ggatgatgtt ttattctcaa gtaaataaag gaaagatgat gagcctacta
      661 atgtctttaa agatatagtg gattaaattt aaaccggtac ggcgttgcct cgccttgccg
      721 tactatttgt actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata
      781 ttttaaacaa caaagaattg aatttggaag aacggcatga tttggagcgg gcggttaaga
      841 tcttgcagga cacgcacgtc atcccggttt gtcggagaaa gatgcatacg ccgtgatggt
      901 cggataccga cactttaaaa gcatccgacc ccatgccttc tgaagattca aaacgcttca
      961 gacggcatat tgaagatatt tctgatattt ctgttgatat ttctttgact tgtcggatat
     1021 aatgtctgac ttggtacatt cgtgccaagt ttaactttgt ctgaaagaca ggccaatcgt
     1081 agcctgtccc tttactttaa aaggaaaata atcatgactt taggtctggt tgggcgcaaa
     1141 gttggtatga cccgcgtgtt cgacgaacaa ggtgtttctg ttccggtaac cgttttggat
     1201 atgtctgcca accgcgttac acaagtaaaa tccaaagatg ctgacggcta tactgccgtt
     1261 caagttacct ttggtcagaa aaaagctaac cgtgtcaata aagccgaagc cggacacttt
     1321 gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa
     1381 ctggctgaat tgaaggccgg tgacgaaatt accgtttcta tgtttgaagt cggtcaactg
     1441 gttgatgtaa ccggtacctc taaaggtaaa ggtttctccg gtacgatcaa acgtcataac
     1501 ttcggtgctc aacgtacttc ccacggtaac tcccgttctc accgtgttcc aggctctatc
     1561 ggtatggcgc aagacccggg tcgcgtgttc cctggtaaac gcatggccgg tcaatacggt
     1621 aacaccaaag caactgtcca aaaactggaa gttgtacgtg ttgatgtaga acgccaactg
     1681 ctgttggtca agggtgctgt tccgggtgcg gtcaacagcg atgttgtagt tcgtcctagc
     1741 gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca
     1801 gtctgtctgt ttctgatgct ttgttcgctc gcgaatacaa tgaagcgttg gttcatcagc
     1861 tggtaaatgc ctacctggca aacgcccgct ctggtaaccg cgctcaaaaa acccgtgccg
     1921 aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg taccggccgt gcacgttccg
     1981 gtatgacttc ttctccgctg tggcgtaaag gtggtcgcgc gttcccgaac aaacctgacg
     2041 aaaacttcac tcaaaaagta aaccgtaaaa tgtaccgtgc tggtatggcg actattctgt
     2101 cccaattggc tcgtgacgag cgtttgtttg ctatcgaagc gttgactgct gaaactccta
     2161 aaaccaaagt ttttgctgaa caagtgaaaa atctgggtct ggagcaagtg ttgtttgtaa
     2221 ccaaacagct cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtgttggttt
     2281 tggaagctca acaagttgat ccttacagct tgctgcgtta caaaaaagta atcatcacta
     2341 aagatgcagt tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt
     2401 gattttggca cctatcgttt ctgaaaaaag caacgtattg gctgaaaaac gcaaccaaat
     2461 gacgtttaaa gtcttggcaa atgcaaccaa acctgaaatc aaagctgctg ttgagctgct
     2521 gtttggtgtt caagttgctt ctgtaactac cgttacaact aaaggcaaaa ctaagcgttt
     2581 tggtcgtact ttgggccgcc gcagcgatgt taaaaaagct tatgtaagct tggcagccgg
     2641 tcaagagttg gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca
     2701 atcgttaaaa tgaagccaac ttctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa
     2761 ggtttgcaca aaggtgcgcc ttatgcacct ttgctcgaaa agaaaaattc tactgccggt
     2821 cgtaaccata atggtcatat caccacccgt cataaaggcg gcggtcataa acaccattac
     2881 cgtgttgtag actttaaacg taacaaagac ggtatccctg caaaagtaga gcgtatcgaa
     2941 tacgatccta accgcactgc ctttattgca ttgttgtgct atgcagacgg cgaacgtcgc
     3001 tacatcatcg ctcctcgcgg tattcaagcc ggtgctgtat tggtttccgg tgctgaagct
     3061 gccatcaaag taggtaacac cctgccgatc cgcaatattc ccgttggtac gactatccac
     3121 tgtatcgaaa tgaaacctgg taaaggtgca caaattgcac gttctgccgg tgcttctgcg
     3181 gtattgttgg ctaaagaagg tgcatacgct caagtccgtc tgcgctctgg cgaagttcgt
     3241 aaaatcaacg tagattgccg tgcgaccatc ggtgaagtcg gtaacgaaga gcaaagtctg
     3301 aaaaaaatcg gtaaagccgg tgctaaccgt tggcgcggta ttcgtccgac cgtacgcggt
     3361 gttgtcatga atcccgtcga tcacccgcat ggtggtggtg aaggccgtac cggcgaagcc
     3421 cgcgaaccgg ttagtccatg gggtactcct gctaaaggct accgcactcg taataacaaa
     3481 cgcacggata atatgattgt tcgtcgccgt tactcaaata aaggttaatt agtatggctc
     3541 gttcattgaa aaaaggccca tatgtagacc tgcatttgct gaaaaaagta gatgctgttc
     3601 gcgcaagcaa cgacaaacgc ccgattaaaa cctggtctcg tcgttctacc attctgcctg
     3661 attttatcgg tctgaccatt gccgtgcaca acggtcgcac ccatgtgcct gtgtttatca
     3721 gcgacaatat ggttggttat aaattaggcg aattctcatt gacccgtacc tttaaaggcc
     3781 acctggccga taaaaaggct aaaaagaaat aaggtgaatc atgagagtaa atgcacaaca
     3841 taaaaatgcc cgtatctctg ctcaaaaggc tcgtttggta gctgatttga ttcgtggtaa
     3901 agacgttgcc caagctttga atattttggc tttcagccct aaaaaaggtg ccgagctgat
     3961 caaaaaagta ttggagtcag ctattgctaa tgctgagcat aataacggtg cggacattga
     4021 tgaactgaaa gtggtaacta tctttgttga caaaggtcca agcttgaaac gttttcaagc
     4081 tcgcgccaaa ggtcgcggta accgcatcga aaaacaaact tgtcatatca atgtgacagt
     4141 gggtaactaa ggaaaagcta tgggacaaaa gattaaccct acaggctttc gcctggcggt
     4201 aactaaagac tgggcttcaa aatggtttgc taaaagtacc gacttttcta ctgttttgaa
     4261 gcaggatatc gatgttcgca attatttgcg tcaaaaattg gccaatgctt cggttggtcg
     4321 agtaattatt gaacgccctg caaaatctgc acgcattacc attcactccg ctcgtccggg
     4381 tgtggttatc ggtaaaaaag gtgaggatat cgaagttttg aaacgtgact tgcaagtctt
     4441 gatgggtgta cctattcatg taaatattga agagattcgc cgtcctgagt tggatgctca
     4501 aattattgct gatggtatcg cccagcagtt ggaaaaacgc gttcaattcc gtcgtgctat
     4561 gaaacgagca atgcaaaatg caatgcgttc tggtgctaaa ggcattaaga ttatgacttc
     4621 aggccgtctg aatggtgcgg atattgcccg tagcgaatgg tatcgtgaag gtcgcgtgcc
     4681 actgcatact ttacgtgcaa atgtagatta tgcaaccagc gaagcgcaca ccacatatgg
     4741 tgtattgggt ctgaaagttt gggtttatac ggaaggcaat attaaatctt ccaaacctga
     4801 acatgagagt aaacaaagaa aggcaggtag acgtaatgct gcagccaact agactgaaat
     4861 accgtaagca acaaaagggt cgcaataccg gcatcgctac tcgcggtaat aaggtaagtt
     4921 tcggtgagtt cggcttgaaa gccgtaggtc gtggtcgttt gactgcccgt caaatcgaag
     4981 ctgctcgtcg tgcaatgacc cgtcacatca aacgtggtgg tcgtatttgg attcgtgtat
     5041 tccctgacaa accgattact gaaaagccta ttcaagttcg tatgggtggc ggtaaaggta
     5101 acgtggaata ttacattgcc gaaattaaac caggtaaagt gttgtatgaa atggatggcg
     5161 ttccagagga actggctcgt gaagcattcg agttggctgc tgccaaattg cctattccta
     5221 caacctttgt agtaagacag gtgggtcaat aatgaaagca aatgaattga aagacaaatc
     5281 cgttgagcag ttgaatgcag atttgttgga cttgttgaaa gctcagtttg gcttacgtat
     5341 gcaaaacgct accggtcaat taggcaaacc aagtgaattg aaacgtgtac gtcgcgatat
     5401 tgctcgtatt aaaaccgttt taactgaaaa aggtgctaag taatgagcga aactaaaaat
     5461 gttcgtactt tgcaaggcaa agtagtaagc gacaaaatgg ataaaaccgt aacagtattg
     5521 gttgagcgta aagtaaaaca ttcgctgtat ggtaagatta ttcgattatc tactaaaatc
     5581 catgcccatg atgaaaataa tcaatatgga attggtgatg tggttgttat atcggaatcc
     5641 cgtccattgt caaaaactaa atcttgggtt gtcagtgagc tggttgagaa agcacgttct
     5701 atttaagaat taaagcaacg tgcttggaat gggaaacgaa gtattgcagc aaatttaatt
     5761 tgcgtgtaaa cttcgtttcc tgtctttcag tttcttctgg aagtttcttc cctttcggga
     5821 tccaagactg gtttacttga accgcaaggt ttcatttaat aagcagcggc tttgctgtaa
     5881 gttatctgaa agtggtaaat taagttggtt aatttaaagg taataatatg attcaaatgc
     5941 agaccatctt agatgtggct gataactctg gtgcgcgtcg cgtaatgtgt atcaaggtat
     6001 tgggcggatc taagcgtcgc tacgcttctg ttggcgatat tattaaagtg gcagttaaag
     6061 atgcggttcc gcgtggccgt gtcaaaaaag gcgatgtata taatgcggta gttgttcgta
     6121 ctgctaaggg tgtgcgtcgt cctgatggtg cgttaattaa attcgataac aatgccgccg
     6181 tgttgctgaa taataaactt gaacctttgg gtactcgtat ctttggtccg gtaacccgtg
     6241 aattgcgtac tgagcgattt atgaaaatcg tttcattggc acctgaagta ttataaggaa
     6301 tagcacgatg aataaaatca ttaaaggcga tagggttgta gtaattgctg gtaaggataa
     6361 aggtaagcag ggtcaagtag ttcgagtgtt gggtggtaaa gttgttgttg agggcgttaa
     6421 tgttgtaaaa cgccatcaaa aacctaatcc aatgcgtggc attaagggcg gtattattac
     6481 taaagaaatg cctttggata tttctaatat cgcaatcctg aatccggaaa ctaataaagc
     6541 ggaccgtgtt ggtattaagc tgattgaaaa tgaaggcaaa gttaaacgcg ttcgtttctt
     6601 caaatcaaat ggctctatca ttggagcata aggagataac atggctcggt tgagagagtt
     6661 ttataaagag acagttgttc ctgaattggt taaacaattt ggttacaaat cagtaatgga
     6721 agtcccgcgt attgaaaaaa tcaccttgaa tatgggtgtg ggtgaggctg ttgctgacaa
     6781 aaaagttatg gaacatgctg tttccgattt agagagaatt gccggtcaaa gaccggttgt
     6841 tactgttgcc cgtaaatcta tcgcaggttt taaaatccgt gataactatc cggttggttg
     6901 caaagtaaca ttgcgtcgtg atcaaatgtt tgaattcttg gatcgtttga ttactattgc
     6961 attacctcgc gtacgtgact tccgtggtgt gagcggtaaa tcatttgatg gccgtggcaa
     7021 ttacaatatg ggtgttcgtg agcaaattat tttcccggaa attgaatacg ataaaattga
     7081 tgctttgcgt ggtttgaata ttactattac tactacagca aaaaccgatg aggaagcgaa
     7141 agctttattg tcattgttta aatttccgtt caaaggataa tcatggctaa gaaagcactt
     7201 attaatcgtg atctgaaacg tcaagctttg gctaaaaaat atgcggctaa acgcgcggca
     7261 attaaagcgg taatcaatga ttcgaatgca actgaggaag agcgttttga ggctcgtttg
     7321 aggtttcaat ccattcctcg taatgcggca cctgtgcgtc aacgtcgtcg ttgtgctttg
     7381 acaggtcgcc ctcgtggtac tttccgtaaa tttggtttgg gtcgtattaa aatccgtgaa
     7441 atcgccatgc gtggcgaaat tccgggtgtt gttaaagcca gctggtaata ggagtaatta
     7501 agaatgagta tgcatgatcc tatttccgat atgttgactc gtatccgcaa tgcgcaacgt
     7561 gctaataaag cagcagttgc aatgccttct tcaaaattaa agtgtgctat tgcaaaggta
     7621 ttgaaagaag aaggatatat tgaggacttc gcagtttcat ctgacgtaaa gtctatattg
     7681 gaaattcaat taaaatacta tgcaggtcgt cctgtaattg aacaaatcaa gcgtgtatct
     7741 cgccccggtt tgcgtattta taaagcgtct agtgagattc caagtgttat gaatggcttg
     7801 ggtattgcta tcgttagtac ttctaaaggt gtaatgactg atcgtaaagc acgttctcaa
     7861 ggtgttggtg gtgagttgtt atgcattgta gcctagtgga ggaaaagaaa tgtcacgtgt
     7921 cgcaaaaaac ccagtgacgg ttcccgctgg tgtagaagta aaatttggaa cagaggcatt
     7981 ggttattaag ggtaagaacg gtgaattgtc ttttcctttg cattctgatg tagccattga
     8041 atttaatgat ggcaaattga cttttgttgc gaataatagc agtaaacaag caaatgcaat
     8101 gtctggtact gctcgcgcat tagtcagcaa tatggttaaa ggtgtttcag aaggttttga
     8161 gaagaaatta caattgatag gcgtgggtta tcgtgctcaa gcacaaggta aaatcttgaa
     8221 tctgtctttg ggtttttctc atccgatcgt atatgaaatg cctgaaggtg tctccgttca
     8281 aactcctagc caaacagaga ttgttttaac tggctcggat aaacaagttg ttggtcaagt
     8341 tgcttctgag attcgtgcgt tccgtgctcc tgagccttat aaaggtaaag gtgttcgcta
     8401 tgtaggcgaa gtagtggtaa tgaaagaagc caagaaaaaa taattgaggt tcactaatgg
     8461 ataaacatac aacccgactc cgtcgtgcac gcaaaacccg tgctcgtatt gcggacttga
     8521 aaatggtaag attatgtgtg ttccgaagca ataatcatat ttatgctcaa gtaattagtg
     8581 ctgaaggtga taaagtattg gctcaagcct ctacattgga agctgaggtg cgcggtagtc
     8641 tgaaatctgg aagcaatgtt gaagcagctg caatagttgg taaacgtatt gctgagaagg
     8701 ctaaagcagc aggtgtagaa aaggttgctt ttgatcgttc aggtttccaa tatcacggtc
     8761 gtgtgaaggc tttggctgaa gctgctcgtg aaaatggttt aagcttctaa atatttggag
     8821 actttcagat ggcaaaacat gaaattgaag aacgcggtga cggtctgatt gaaaagatgg
     8881 tcgcagttaa ccgtgtaacc aaagtagtta aaggtggtcg cattatggct ttctctgcgc
     8941 taactgttgt tggtgatgga gatggtcgta ttggtatggg taaaggtaag tcaaaagaag
     9001 tgcctgttgc agtccaaaag gcgatggatc aagcacgacg ctctatgatt aaggtaccat
     9061 taaaaaatgg tacgatccat catgaggtta ttggtcgaca tggtgctact aaagtattta
     9121 tgcagcctgc taaagagggt agtggcgtaa aagccggtgg acctatgcgt ttggtttttg
     9181 atgctatggg cattcataat atttccgcca aagtgcacgg atctactaac ccatataata
     9241 tcgtacgtgc aacattagat ggtttgtcta agttgcatac tcctgctgat atcgcagcca
     9301 aacgtggctt gacagtggaa gacattttgg gagttaacca tggctgaaca aaaaaagatt
     9361 agggttacat tggttaaaag cctgattggt acaattgaat ctcatcgtgc atgtgcacgc
     9421 ggtttaggtt tgcgtcgtcg cgagcatacg gtagaggttt tagatacctc tgaaaaccgt
     9481 ggtatgatta ataaaatcag ctacttgttg aaagtggagt cttgatatgt ttttgaatac
     9541 aattcaacct gctgttggtg ctacgcatgc tggtcgtcgt gttggacgcg gtattggtag
     9601 tggtcttggc aaaacgggtg gtcgtggtca taaaggtcaa aagagccggt ctggtgggtt
     9661 tcataaggtg ggtttcgagg gtggtcaaat gcccttgcaa cgacgcctcc ctaaaagagg
     9721 ttttaaatct ttaacagtat cagctaatgc acagcttcgt ttaagtgaac tggaatcaat
     9781 tgctgttaat gagattgata ttttagtctt aaagcaagca ggtctgattg catctacagt
     9841 ctctaatgtt aaagttattg cttctggtga aatttctaag gcagttgctt tgaagggtat
     9901 taaagttacc aaaggtgcga gagctgctat cgaggatgtt ggtggtaaga ttgaaatgta
     9961 aggtttaata ttgtggctaa tcaacaaacg tcatcaggtt catccaagtt tggagatctt
    10021 aagaaacgtc ttttgtttct atttggagca ttgattgttt ttcgaattgg tgcccatata
    10081 cccgtacctg gggttgatgc tgttgcttta gctaaattat acgaaagcgc tggaaacggc
    10141 atcctgggaa tattgaatat gttttccggt gggtcgttag agcgctttag tatatttgca
    10201 ataggaatta tgccatatat ttcagcttct attattgtac agctcgcttc tgaaattttg
    10261 ccatcattga aggctttaaa aaaagaaggg gaggctggta gaaaggtaat tacgaaatat
    10321 actaggtatg gtactgtttt gttagcaatt cttcaaagtc taggtgttgc atctttcgta
    10381 tttcagcaag gaattgttgt aacaagttca tttgagtttc atgtttccac ggtagtttct
    10441 ttggtaacgg gaaccatgtt tcttatgtgg cttggggagc aaattactga aaggggtatc
    10501 gggaacggta tttctttaat cattacggca ggtattgctt caggtattcc ttcgggtatt
    10561 gcaaagctgg ttacactgac gaaccaaggt tctatgagca tgcttacggc gttgcttatt
    10621 gtatttggtg ccttattatt aatttatttg gttgtatact ttgaaagtgc acagcggaag
    10681 attcctattc attatgcaaa acgccagttt aatggtaggg tgggtagtca aaatacgcat
    10741 atgcctttca agttgaatat ggctggtgtt attcccccaa tttttgcttc cagtattatt
    10801 ctatttccat ctactctttt aggttggttt ggttcggctg atacaaatag tgttttgcac
    10861 aaaatagctg gattgttaca acacggtcaa ttgctgtata tggctttatt tgcagcgaca
    10921 gttattttct tttgttattt ttatacggct ttggttttta gtcctaaaga aatggcagag
    10981 aatttaaaaa agagtggtgc ttttgttcct gggattagac ctggtgagca gacctctagg
    11041 tatttagaaa aagttgtatt acgtttgaca ttgtttggag ctctttatat tacaactatt
    11101 tgtttaattc cagagttctt aactacggtt ttaaatgtac ctttttattt gggtggcacg
    11161 tctttgttga ttctagttgt tgtaacgatg gattttagta cacaaataaa ttcgtatagg
    11221 cttactcaac agtatgataa gttaatgact cgttcagaaa tgaaatcatt ttctcggaaa
    11281 tagaattatg gcgaaagaag atactatcca aatgcaaggt gaaattcttg aaactttacc
    11341 taacgcaaca tttaaagtaa aacttgagaa tgaccatatt gtattgggtc atatttctgg
    11401 gaagatgcgg atgcattaca ttcgtatttc tccgggagat aaggtcacag tagagctgac
    11461 accttatgat ttaactaggg ctcgaatcgt tttcagagca agataaacca ataaaaggaa
    11521 aataaaatgc gtgtacaacc atctgttaag aaaatttgcc gaaattgcaa gattattcgt
    11581 cgaaatcgtg tagttcgtgt gatttgtact gatccccgtc acaaacagcg tcaaggttaa
    11641 tggaatattt cttttaatgt gattctgtga tatagtgaca aaatttgctc taaaaaggaa
    11701 aaaacatggc tcgtattgca ggggtaaata tccctaataa cgcacacatc gtaattggtc
    11761 ttcaggctat ttacggtatt ggtgctactc gtgctaaatt gatttgtgag gctgcaaata
    11821 ttgcgcctga tactaaagca aaagatttgg acgagactca attagatgct ttgcgtgatc
    11881 aagttgccaa gtatgaagta gaaggtgatt tgcgtcgtga agtaactatg agtatcaaac
    11941 gattgatgga catgggctgc tatcgtggct tccgtcatcg tcgcggctta ccatgccgcg
    12001 gtcaacgcac tcgtacaaat gcgcgtactc gcaaaggtcc gcgtaaagcg attgctggta
    12061 agaaataaat tttaaggaat tttattaatg gctaaagcaa acacagcttc acgtgtacgt
    12121 aaaaaagtac gtaaaaccgt gagtgagggt attgtgcacg ttcatgcatc tttcaacaat
    12181 accatcatta caatcactga ccgtcaaggc aatgcgttgt cttgggctac ctctggcggc
    12241 gctggtttta aaggttctcg taaaagtaca ccatttgcag cacaagttgc agcagaagca
    12301 gctggtaaag ttgcccaaga gtatggcgtt aaaaatttag aggttcgtat taaaggtcca
    12361 ggtccaggtc gcgaatcctc tgtacgtgct ttgaatgctc ttggtttcaa gattaccagc
    12421 attactgacg ttaccccgtt gcctcataac ggttgccgtc cgcctaaaaa acgtcgtatt
    12481 taatattgga gtgatttgaa acatggcacg ttatattggc cctaaatgta agttggcacg
    12541 tcgcgaaggt acggatttgt ttttgaagag tgcgcgccgc tctttggatt ctaaatgtaa
    12601 aattgattcc gctcccggtc agcatggtgc aaaaaaaccg cgtttgtcag actatggttt
    12661 gcagttgcgt gaaaaacaaa aaatccgccg tatttatggc gtattagaac gtcagttccg
    12721 tcgttatttc gcagaagctg atcgccgtaa aggttctact ggcgagttgc tgttgcagtt
    12781 gctggaatct cgtttggata atgtcgttta tcgtatgggt ttcggttcta cccgagctga
    12841 agcaagacag cttgtttctc ataaggctat agttgtaaat ggacaagttg tcaatattcc
    12901 ttctttccaa gtgaaagctg gtgatgttgt ctcagttcgt gaaaaagcca aaaaacaggt
    12961 acgtattcaa gaagcattgg gtttggcaac tcaaatcggc ttgccgggtt gggtttctgt
    13021 agatgcagat aaacttgagg gtgtgttcaa aaacatgccg gatcgctctg aattgaccgg
    13081 tgatattaat gaacagctgg tggtagagtt ctactctaaa taatgctagc tcagtgaggg
    13141 acagttaaat gcagaatagc acaaccgaat ttttgaaacc tcgtcaaatt gatgtaaata
    13201 ctttttctgc aactcgtgca aaagtatcta tgcagccatt tgaacgtggt ttcggtcata
    13261 ccttaggtaa tgctttgcgc cgtatcttac tgtcatccat gaatggtttt gctcctactg
    13321 aagtagctat tgccggtgta ttgcacgaat attctactgt tggtggtgtt caggaagatg
    13381 ttgttgacat tttgctgaat attaaaggta ttgtgtttaa actccatggt cgtagccaag
    13441 ttcaacttgt gttgaagaaa tcaggttcag gtgtcgtatc tgccggtgat attgagttgc
    13501 cgcatgatgt agaaattctg aatcctggtc atgtcatttg tcatttggct gataacggtc
    13561 aaattgaaat ggaaattaaa gtagagcaag gtcgtggtta tcaatctgtt tcaggtcgtc
    13621 aggtagttcg tgatgagaac cgtcagattg gtgcaatcca gttggatgcg agcttttcgc
    13681 ccatcagccg tgttagcttt gaggttgaac ctgcacgtgt agagcagcgg acggatcttg
    13741 ataaattggt tttggatatc gaaaccgacg gttctattga tcctgaggaa gctgtacgca
    13801 gtgcggcacg tattttgatt gatcagatgt ctatttttgc tgatttgcag ggtactcctg
    13861 tggaggaggt tgaagaaaaa tcacctccta tcgaccctgt tcttttgcat ccggtggatg
    13921 atctggaatt gacagtacgt tcagctaatt gtttgaaagc tgaggatatt tattatattg
    13981 gcgatttgat tcaacgcact gaaaccgagc ttcttaaaac gccgaatttg ggacgtaaat
    14041 ctttgaatga gattaaggaa gtattggcat ctaaaggttt gacactgggt tctaagttgg
    14101 aagcatggcc acctgtaggc ttggaaaagc cttaatgaag aattaaagga taattgatat
    14161 gcgtcatcgt aatggcaatc gcaaattaaa ccgtaccagc agtcatcgtg ctgcaatgct
    14221 gcgtaatatg gcgaattcat tattgactca cgaaactatt gtaacaactc tgcctaaggc
    14281 caaggaattg cgccgtgtag tagagccgtt gattacattg ggtaaaaagc cgtcattggc
    14341 aagccgccgt ttggcatttg accgtactcg cgaccgtgat gttgtagtaa aactgtttgg
    14401 cgatttgggt cctcgtttta ctgctcgtaa cggtggttat gttcgagtgt tgaaatacgg
    14461 attccgtaaa ggtgataatg cacctctggc actggttgaa ttggttgaca aaccggctgc
    14521 tgagtaattt tagtcatata acgccatctg ccgaaaagca ggtggcgtta tttttgcaat
    14581 atctgataga taatatggta ttggctatta tgtttaaaat attaattgaa tagctaaggt
    14641 ttgcgcggta aacttacatc attaaaaaat tctatgatgg tttatataat gaatgccttc
    14701 gatataaagt cgacaaagat ggacgtattg tctatatctt tgcatacatc agacttgttt
    14761 gatttggaag atgtgctggt caaattgggc aagaagtttc aagagtctgg tgttgttcca
    14821 tttgttctgg atgttcaaga atttgattat cccgagtctt tggatcttgc tgcattggtt
    14881 tcgttgtttt caaggcatgg tatgcaaatt ttgggtctga agcattctaa tgaacgttgg
    14941 gctgctgtgg ctatgaagta tcatttgctg ttttgtctgt ctcattcgga aaatgttaaa
    15001 gaactgggtc aggttgaggt gcagaaaacg gaggatggtc agaaagcaag gaaaacagta
    15061 ttgattacat cccctgtccg taccggtcag caggtttatg ccgaagatgg cgatttgatt
    15121 gttacggggg cggtcaacca gggggcggaa ttgattgcag atggcaatat gcatatttat
    15181 gcgccgatga gagggcgtgc tttggccggc gccaaaggtg atacttctgc ccgcatattt
    15241 atccactcca tgcaggcgga gttggtttct gtcgcgggga tttaccgtaa ttttgaacag
    15301 gatttgccgg accatctgca caagcagccg gtacagatat tgttgcagga taaccgattg
    15361 gttatcagtg caattggctc agagtaattg tttgatattt taaaaaggaa atattgtggc
    15421 aaaaattatt gtagtaactt caggcaaggg cggtgtcggt aaaacgacta ccagtgcaag
    15481 tattgcaaca ggtttggcat tacgcggata taaaactgcg gtaattgatt ttgatgtggg
    15541 tttgcgcaac ctcgacctca ttatgggttg cgagcgtcgt gtcgtttatg acctgatcaa
    15601 tgtcattcag ggtgaggcga cgctcaacca ggctttgatt aaagataaaa attgtgaaaa
    15661 cctgtttatt ttgccggctt cccagactcg ggataaagac gctttgacac gcgagggcgt
    15721 agaaaaagtg atgcaggagc tgtccggcaa gaaaatgggc tttgagtata ttatttgcga
    15781 ctctcctgct ggtattgagc agggtgcatt gatggcgttg tattttgctg atgaagccat
    15841 tgtaacgacc aatcctgagg tttccagtgt gcgtgactcc gacaggattt tgggcatttt
    15901 gcaaagcaaa tcccgtaagg cggagcaagg cggttcggtt aaagaacatc tgttaattac
    15961 gcgttattct cccgaacgtg tggcaaaagg cgaaatgctg tctgtacagg atatttgcga
    16021 tattctgcgt attcctttgc tgggtgtgat tcccgaatcc caaaacgtct tgcaggcatc
    16081 caattccgga gaaccggtca tccatcagga cagcgtagcg gcttccgagg catataagga
    16141 cgttattgcc cgtcttttgg gcgagaaccg tgaaatgcgt ttcttggaag ctgagaaaaa
    16201 aagcttcttc aaacgtctgt tcggaggata aggtatgtca ttgatcgaac ttttattcgg
    16261 tagaaagcag aaaacggcaa ccgttgcccg cgaccgcctt caaatcatca ttgcccaaga
    16321 gcgcgcccaa gaaggtcaga ctccggatta cctgccgact ttacgtaaag agttgatgga
    16381 agtcctgtcc aaatatgtga atgtttcatt agacaatatc cgtatttccc aagaaaagca
    16441 ggatggtatg gatgtgcttg agttgaacat tactttgccg gaacagaaaa aggtatagga
    16501 catgacctta accgaattgc ggtacatcgt cgcagtcgcc caagaacgtc atttcggccg
    16561 ggcggcgcgg cgttgttttg tcagccagcc cactttgtct attgccatta agaaattgga
    16621 agaagagctt gccgtctctt tgtttgaccg gagcagcaac gatattatta cgaccgaggc
    16681 gggggaacgt atcgttgcac aggcgcgtaa ggtattgaaa gaggcggagc ttatcaggca
    16741 tttggcaaat gaagaacaaa acgagctgga gggtgcgttc aaactcgggc tgatttttac
    16801 ggttgcgcca tacctgctgc cgaaactgat tgtctctttg cgccgtactg caccgaaaat
    16861 gcctttgatg ttggaagaga attacacgca tactttgacc gagtcgctca aacgcgggga
    16921 cgttgacgcg attatcgttg ccgaaccgtt tcaagagccg ggcattgtta ccgaaccctt
    16981 gtatgacgaa ccgtttttcg tgattgtccc gaaagggcat tcatttgagg aactggatgc
    17041 cgtttcgccc cggatgctgg gtgaggagca ggttttgctg ctgacggaag gcaactgtat
    17101 gcgggatcag gtactctcaa gctgttctga attggcggcg aaacagcgca tacaggggct
    17161 gaccaataca ttgcagggca gctcgatcaa tacaatccgc catatggtcg ccagcggttt
    17221 ggcaatcagc gtgttgccgg caaccgcgct gaccgagaac gatcatatgc tgttcagcat
    17281 tattccgttt gaaggtacgc cgccaagccg gcgggtcgta ttggcgtatc gccgtaattt
    17341 tgtccgtccg aaggcgttgt cggcgatgaa ggcggcgatt atgcagtcgc agcttcacgg
    17401 ggtaagtttt atccacgact aggcgcagaa aatgccgtct gaaacgggtt tcagacggca
    17461 tttgttatct ttgccgtcaa tcaggcaaac ctatccgacc tacagttgct tcgaatcgtc
    17521 attcccgcgc aggcgggaat ctaggtctgt cggtgcggaa acttatcgga taaaacgctt
    17581 gctccaatct cgcgttccag attcccactt tcgtgggaat gacgggatca ggtttccgta
    17641 tggatagatt cgtcattccc gcgcaggcgg gaatctaggt ctgtcggtgc ggaaacttat
    17701 caggtaaaac ggtttctccg gtcccgagtc ctagattccc actttcgtgg gaatgacggc
    17761 atattttgac a
//
LOCUS       DAWTCI010000043        17590 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWTCI010000043 DAWTCI010000000
VERSION     DAWTCI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17590
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.043"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            404..571
                     /locus_tag="WM481_001357"
     CDS             404..571
                     /locus_tag="WM481_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080665.1"
                     /translation="MVNEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNLDFSQW
                     VKSIIKEKIKK"
     gene            731..1582
                     /locus_tag="WM481_001358"
     CDS             731..1582
                     /locus_tag="WM481_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFA9080666.1"
                     /translation="MNAVQVLNFQQNSVRTVADNKGELWFLANDVCEILGYTNPRRTV
                     DLHCKSRGVTKRYTPTASGEQEMTYINEPNLYRLIIKSRKPAAEAFEEWVMETVLPAI
                     RKTGGYQITPKTTADDRTGLRQAVAALVGRKGIDYYSAYSMIHQRFNVESVEGIPAGK
                     LPEAVAYVHALTLHTGLTGEVLDREPLPAPQPALPIDGNALAGIAAMVYYGTWMIELG
                     KDISAPLKQLGNRQAVTMWTVWHETRSILKRSAAALEVLRGYADKDTSDRIAACLEGI
                     YRKAAAR"
     gene            complement(1768..2442)
                     /locus_tag="WM481_001359"
     CDS             complement(1768..2442)
                     /locus_tag="WM481_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080667.1"
                     /translation="MNLKALIARFRVLANDKAEPYFWSDEEVSGWLNDAVHEACLRGR
                     LLHSDDAFVTDVEKGRPLYAYAAGGFAAGYAYEIDSIRFVSDGKPVCLKLVSPEAADV
                     CAPGWRDGAQTGLPVYAVQGDGKLTLAPAPDRDGRLFAGGYCLPRDMAGDGDEPEINS
                     IHHRNLVYWALAEAFSIPDAETFDPQRSESARRRFELYFGLPADSDLRRITREDTPHL
                     NRHFWI"
     gene            complement(2439..2924)
                     /locus_tag="WM481_001360"
     CDS             complement(2439..2924)
                     /locus_tag="WM481_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080668.1"
                     /translation="MKRRPGICLCGVAVFDNAVRTAAGGWMDKVFIKYIGGRAVWRDR
                     IYHTGLVFEDGQVREVSAGAAAKLLRHGDVFAAVPGKRVEKADDTEALEKAGASELER
                     ETAAFDAVQDVILQINRMGKDELELYAKANYGQGLDKRKSAENLRGDVVRMVRQFGIA
                     Q"
     gene            complement(2930..3403)
                     /locus_tag="WM481_001361"
     CDS             complement(2930..3403)
                     /locus_tag="WM481_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080669.1"
                     /translation="MAEIYVKNNGGNRFGGVPYGNLAAEHYRIVAKQDGAILGADASE
                     PPKAGDVLALGVLEQGFRLDDAQIIVKTAMSSGITADVGFAYADGADDAKVPQDAAYF
                     ASGADFASAGRIRCQSAKLVTLPKQALLTVTLKGAENKKAADIDILIYGEKFGQL"
     gene            complement(3458..4753)
                     /locus_tag="WM481_001362"
     CDS             complement(3458..4753)
                     /locus_tag="WM481_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFA9080670.1"
                     /translation="MAQKTNTAYGDPQAMMKQAAGLFAMHMQRNSTLNRLAGKMPAGT
                     AGAEATLRKQTTQHMPVVRCQDLTRGMGDEIRFNLVNPVSALPIMGDNTAEGRGVGMS
                     LSEAGLRVNQARFPVDGGGTMTNQRSPADYRALIRPAAQSLMDRYADQTLLVHMAGAR
                     GFHDNIEWGVPLAGDPKFNDYAVNPVKAPSKNRHFTASGDAVTGVGDNGGELKIASTD
                     LFTMDTVDSMRTVLDQIPLPPPIVKFEGDKAAGDSPLRVWLLSPAQYNRFAADPKFRQ
                     LQASAIARASQANQNPLFLGDAGLWNGFILVKMPRPIRFYAGDEMKYCADKFSEAESG
                     LKIPASFADKFAVDRSVILGGQAVLEAFANTGKHGGMPFFWSEKELDHGNRVETLVGT
                     IRGVAKTRFAVDVGGGAKEITDYGVTVVDTVVPLHGGIR"
     gene            complement(4774..5296)
                     /locus_tag="WM481_001363"
                     /pseudo
     CDS             complement(4774..5296)
                     /locus_tag="WM481_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5389..12690)
                     /locus_tag="WM481_001364"
     CDS             complement(5389..12690)
                     /locus_tag="WM481_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HFA9080671.1"
                     /translation="MSDLVRYDPLEHGRLAGGLKEYRGFTQKDARAAADDTALTRGFK
                     NSMRSARMGWNALTGDKEELGRLKAEDMDYRKIQEGRKSQARRELGEAWEKGGGVGGG
                     LSNVWGELKKDWREKGLDGALEDVGEMGGAVLEQAPNALVPIATTTAGGILGALAGGN
                     AAVGAYAGATLGNTLMEYGGQLDRAAEAAGVDPADKDAVMAFIGRGAPGALKNAAVKG
                     AVVGAADMAAMKLGGSILNMGKKAAGKAALEKMGVAAADKAAVAAAKGTPEFAALAKE
                     SAKGGLGGAARHAAAYATESAGEFAGEYLGTGLANGEWDEKGAALEAFSSLGHSAVGF
                     AGTKAYAAVTDPLRPPAGRKAGAQGGIGGNRKAGRPAPEGAQALRAAAQAAADGGAEQ
                     GGAGFDTAHHDQSHPALRQFADRTKQEEAGRFFSGPADGNTPHAEELARGTEKQPDVS
                     GIPSEDGAEFLDTGVMPDGLARQYADMAAKYRAKPSEAVGIDPDDGAVSAAAALAADS
                     GAAVPSAVSDDMEARSVADDAPSGRSADADRGGVPSAYGNVRPGGAPRGAASVAPGGS
                     AAAASGGIARVSPLPAGQYFDGLDTRGRKALAKEAGLDIKGVADFGQIAAPVRRKIEQ
                     AYHARIEADYQAASEAKQGYLPPPVRMADAVPVPKKGFSVPADALDKESRRRFDALPE
                     WVRRHAQTVADYTADGIMRREAGMADMRGHYPEGLAESAGAVRAYRAQHPESADVLDR
                     LNRAVYGYRRNNGWSVPLLSREGERLQGVRTALPDDGASEAVVGGGRGLTRALPTEDK
                     GLAQDVRQDVRQGLTQGGRGLTPDAGADANAAALQGLPGSAVASGNAPARRQNLQVRA
                     RAEGAAPGLSASENLAGTDGGKRAPVAGKRPDTVLPVLNPQVAASAGRVSPKKRMADA
                     AADRRRPEKAGVPLGGGEYRFEHTDRRHIDALAGVPGRPGKGGMPEEFADMAGPSNSD
                     GLVSDGRRYLKGREAETLRAGGLSEAVPSEPGRDYRPTQEARAPARVMARPRDAAADG
                     KPAGRAQPARAKDTPVAGKAAAAKNAATEKPSSDKVRNIESGKSHFDGGKGKSAAAQG
                     AATEKPSSDKAAKPETFAKTASDNPEEARRKARVLQGGPVYTVKERQAPQGFKALREH
                     AENIKKRLAESIGGLAERVDVAAVSETAPDKAQMLLSQRVEGWFDGRTGKITLVAENL
                     TPERAVWAAWHELGHRGFAADGFAKYREELERADGNGLIRRIADAVQEGREGTGDAAA
                     SVRSAAVEEAVAELYAAQRTGGWAGIENRYGVKVGNGLKRGIAGVLARIGALLRRVLQ
                     RLAGKAGGAMSDADVFAMLADLHGNVEGARDAPWDGNHRAVMFARAEDGAAERSKSES
                     LEKLRRAETIRISGREVPEGGNLREYKRNALEYGKSLRGPYVNKDTGREISLGRSGIT
                     EILRHDYKDAEHLQSIAAIPQIIENAVYIDTLPNEDLAKNGDIQGYEYYVSGLNVGGA
                     DYTVRAAVAVSRNGNRYYDHKLTKIEKGNLLSLLDRVSTTGASESKSPLSGIDDKRLL
                     QILQDKDAGKGGIADFDTEAVRFSRAANIGAAISRITGKKSDLRNALKDRWDASKGIQ
                     LQFLGRRQIEDIYGGVLDGLKEYGRLSELFGADANKAVTEADKVVREWGRLKEEDAKA
                     LADLMHDATLAKVDADPLMRKDARGRLDGIRTALDIADGKIAKARAAVASAGARTARA
                     DAAYNKAQRAADKAAYALEKAQEKHGREILADEADMRLRRLFYADSEAKRALRRAGAD
                     AAAESRAKTDAVRMLEQARADVKRLEKDEVGAQKALEGLALLNRRFAGLPDAAQRVYR
                     KARDDYRAHFGQVRDALAERLARAGQDAETVRRLKERFDNELGGVYFPLARFGDYLVV
                     VKDADGNSANVSRAETLSEAEKLRDALKADFGPGFKVSPVMKSRDYIRSRDAVGSGFM
                     KELGEAVGRMDLDPAQRARLNDTLTQLYLNSLPDTSWAKHGIHRKGVPGFSDDARRAY
                     AQNMGSGANYLAKLRYADRMAEQLDVMQDFVDGRKYEEGFDQRQLQRVADEMRKRHEA
                     VMNPNPSKLAQALTGFGFLWMMGMSPASAVVNLSQTAMVAYPVMAAKWGYAGAARELL
                     RASKQIGLRFGEKFNTIEDSLNGDEKAAFRKAADYGVIDLSQAHDLAGVANGDPGLAG
                     SAWQKVMDKAAWLFHHAEKFNRQVTFVAAYRLAKRAGADSEAAFEQAKKATYDGHFDY
                     AAQNRPRFMMGNAAKVVFLFKQYSQNILYALGRNAYLAFKGDKEARKTLAGLLVSHAM
                     ASGILGLPFVSTLLAVASMLGSDDDDPWDAEAALRNMLADTFGDKAGEVLAKGFSRLT
                     PLDVSGRLGLDQLVFPDIQDGLEGKKWAESLVVGSTGAVVGAGIGAADGVRTRSSVPR
                     TANTLSLMKSW"
     gene            complement(12687..13883)
                     /locus_tag="WM481_001365"
     CDS             complement(12687..13883)
                     /locus_tag="WM481_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080672.1"
                     /translation="MGLFEPSAGDFWEMKEKEKKEKARKGAEERERAAAQAHRADAVR
                     RTVANYEAGPARYRNVMDLSRNNIEDGARRLRRAGAFERGADAGLGFSGGDKALSPDA
                     RAGADFARRDTRPTDAGGRTPPPLGFDGNVYRDGKPVRDFDAQRPLVSAGPDALSPEE
                     RELYRRATTPHAGALNGQLTAAQLNAARGIVAEHNKNAAVRELGRERLAAAAAENAAN
                     REAVLQEGRFDAAVKANEGALNREMAQRNADRAFDVQQAELGMKRQGFEMKREADALE
                     LEDRKRIADLTRAYGFAKSDGQRGEIARQIDALNGKFERQGEKGFDPNVFKTISYEVA
                     DPDTGLTAKREGIVDLRTGKPLDVEFAGEREKRYAQLGFKPNGQKTAGGKIIYENEKG
                     EKRVEQ"
     gene            complement(14120..14308)
                     /locus_tag="WM481_001366"
                     /pseudo
     CDS             complement(14120..14308)
                     /locus_tag="WM481_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14294..15331)
                     /locus_tag="WM481_001367"
                     /pseudo
     CDS             complement(14294..15331)
                     /locus_tag="WM481_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
     gene            complement(15312..15554)
                     /locus_tag="WM481_001368"
     CDS             complement(15312..15554)
                     /locus_tag="WM481_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080673.1"
                     /translation="MAENLSKSAEQIVAELYGSPEDTFTKARMFQTVAAGFKVLVEIE
                     RKSYGMDTAESKISESAKTAGIRIEFVGPEDDGKDG"
     gene            complement(15612..15851)
                     /locus_tag="WM481_001369"
                     /pseudo
     CDS             complement(15612..15851)
                     /locus_tag="WM481_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15851..16273)
                     /locus_tag="WM481_001370"
     CDS             complement(15851..16273)
                     /locus_tag="WM481_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080674.1"
                     /translation="MSGLSDIDKVYQGVVDLHNAEQVVSREALAETTGLKMSEITKFT
                     KLLVEHGKIYRVTRGIFKPAIGFGETRPVSVSVLDSGMGVLEIGDTVLHLNPQEMRSL
                     GALMSGFGQQFSSIQMGREFSVLRNYLECSAKNGRLDL"
     gene            complement(16338..16856)
                     /locus_tag="WM481_001371"
     CDS             complement(16338..16856)
                     /locus_tag="WM481_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9080675.1"
                     /translation="MGLTQEVLKELLRYDDNTGKLYWAERPRKYFNSGLHYKSWNTGF
                     SGKEVFLYKGRLGYLKLKIFKKQYNAHRLIWLFVYGKHASSIDHINRDKTDNRISNLR
                     DVTHAENMKNRGKFKNNTSGHTGVYFHKPSKKWQARIMVNRKNKILGLFEHIEDAVKA
                     REAASKDFGFVV"
     gene            complement(16847..17230)
                     /locus_tag="WM481_001372"
     CDS             complement(16847..17230)
                     /locus_tag="WM481_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFA9080676.1"
                     /translation="MNGAEFTLTPQNKKQVMRSIWDSPDGWFENGNLEITIRPRKSKR
                     SVEQNRRLWFLYREISEKVFIDGRRFSQDVWHEFLKRKFIGCIEMPNGQLMGISTTKL
                     SVREMSEYQEKIISWASMEHGVLWD"
     gene            complement(17227..17577)
                     /locus_tag="WM481_001373"
     CDS             complement(17227..17577)
                     /locus_tag="WM481_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HFA9080677.1"
                     /translation="MRQTRVGISSDRRVGMSSAIRKAAKGGQCTPNIAGVCNDNPETV
                     VLCRFPGETHGAGLKSGGLGAGFGCGCRRGAIDGRGAGLSREDKEFYMRRSQLRTIRR
                     LEALGVVGVKGRLK"
BASE COUNT         3726 a         6215 c         4000 g         3649 t
ORIGIN      
        1 aaattagttt ttcccaatgg gttaaagaac aaatccgaaa agagttctta aaatagcccg
       61 accgccgcca agccgcaaac ttggcggctt ctttatgccg tccgaaagcc cttcagacgg
      121 catttttctt tgattttgtt gggggaatgg ttgactttga acgtgtccac gtttataatt
      181 caattcatcg gcgcatcggg tgccgatacg ggaaaagccc caccgaagcg gggcttgtac
      241 agggagtagg aaaatgaagc tcatccttca aattctcatc ctggttttaa tcttgatgct
      301 ggcaggcgat acgcctatta gcggataaaa ccggctgggg cggtagccgc cgccccagtc
      361 cctatcttac aaaaggcagt tcaaaaaatc aaggagtttg cctatggtta atgaaaaact
      421 ggcagaaaac cgaaagcgtt acgagcaaaa acgagtcatt aaaaaagtct cgtttaatgc
      481 ggaaacagaa aaagagcttt tggaatatgc ccaaaatctc gatttttccc aatgggttaa
      541 atcaatcata aaagaaaaaa tcaaaaagta attgactttg aacgtgtcca cgtttattat
      601 tcttacaacg gcacagccgt taatggaaaa ccgaaccaag cgggaacttg gttcggttaa
      661 gtgaaaagca gttctaggaa aactcttttc gcgtctttta aataacctct aaaagaaaga
      721 ctattctatc atgaatgctg tccaagtttt aaactttcaa caaaactccg tccgtactgt
      781 tgccgataac aaaggcgagt tgtggttttt ggcaaacgat gtttgcgaga ttttaggcta
      841 caccaaccct cgccgaacgg ttgacctgca ctgcaaaagc aggggcgtaa caaaacggta
      901 cacccccacc gccagcggcg aacaggagat gacctatatt aacgagccga atctctaccg
      961 cttgattatc aaatcccgca agcctgccgc agaagcgttt gaagaatggg taatggaaac
     1021 cgtcctgccc gccatccgca aaacaggcgg ctaccaaatt actcccaaaa ccaccgccga
     1081 cgaccgtacc ggattgcgcc aagctgttgc cgcgcttgtc ggacgcaaag gcatagacta
     1141 ctactccgcg tacagtatga tacaccaacg cttcaacgtc gaatccgtcg aaggcatccc
     1201 cgccgggaag ctgcccgaag ccgtcgccta cgtccacgca ttgacactgc acacgggctt
     1261 gacgggcgaa gtccttgacc gcgaaccgtt acccgcgccg caacccgccc tgcccatcga
     1321 cggcaacgct ttagccggca ttgccgctat ggtttattac ggcacatgga tgattgaatt
     1381 gggcaaagac atctccgcgc cgctcaaaca gcttggcaac aggcaggcgg ttacgatgtg
     1441 gacggtttgg cacgaaaccc gttcaatcct gaaaagatcc gccgcggccc tcgaagtgtt
     1501 gcggggatat gcggacaaag acacctccga ccgcatagcc gcatgtcttg aaggcattta
     1561 cagaaaggct gcggcaaggt aaacggaaaa tgccgtccga accttcggac ggcatttttc
     1621 agacggcata gcgtttcaac atttcccgat aatccgccgg ttcccgtccg gcaaattcaa
     1681 aggccttcaa atcgccgccg cccgcatttc ggcgcccgtc cggccggcag gcggtttgtt
     1741 tttcacaaaa tccttcggcg tccgccgtca aatccaaaaa tgcctgttca ggtgcggcgt
     1801 atcctcacgc gtgatacggc gcaaatcact gtcggccggc aggccgaaat acagctcgaa
     1861 acgcctcctt gccgattccg aacgctgcgg gtcgaaagtc tccgcatccg ggatactgaa
     1921 agcctccgcc aaagcccaat aaaccaaatt ccgatgatgt atggaattga tttccggctc
     1981 gtccccgtcc cccgccatat cccgcggcag gcaatacccc ccggcaaaca acctgccgtc
     2041 acggtccggc gcaggcgcaa gcgtcagctt cccgtcgccc tgcaccgcat aaaccggcag
     2101 ccccgtttgc gccccgtcgc gccaacccgg ggcgcaaaca tccgccgcct ccggcgaaac
     2161 caacttcagg cacaccggct tgccgtcgga cacaaaacgg atactgtcga tttcatacgc
     2221 ataacccgcc gcaaaccccc ctgccgcata agcataaagc ggccgccctt tttccacatc
     2281 cgttacaaac gcatcgtccg aatgcagcag ccggccgcgc aggcacgcct cgtgaaccgc
     2341 atcgttcaac catcccgaca cctcttcgtc gctccaaaaa tacggctcgg ccttatcatt
     2401 ggcaagcacg cggaaacgcg caatcaaagc cttcaaattc attgcgcgat accgaactga
     2461 cgaaccatcc gaaccacgtc cccgcgcaaa ttctccgccg atttgcgctt atccaaaccc
     2521 tggccgtaat tcgccttcgc atacagttca agctcgtcct tgcccatccg gttgatttgc
     2581 aggatcacat cctgcaccgc gtcaaacgcc gccgtttccc gttcgagttc cgaagcgccc
     2641 gccttttcca aagcctccgt atcatccgcc ttttccacgc gtttgcccgg gacggcggca
     2701 aacacatcgc cgtggcgcaa caactttgcc gcagccccgg ccgacacctc gcgcacctgg
     2761 ccgtcttcga aaaccaagcc cgtatgatag atacggtcgc gccacacggc acgcccgccg
     2821 atatacttga taaaaacctt atccatccaa ccccccgccg ccgtccggac ggcattgtca
     2881 aatacagcaa cgccgcaaag gcaaatcccc gggcggcgtt tcattccgct tacaactggc
     2941 cgaacttctc gccgtaaatc aaaatatcga tatcggcggc cttcttattt tccgcccctt
     3001 taagcgtaac cgtcagcaaa gcctgcttgg gcagcgtaac cagtttggca gactggcagc
     3061 ggatacgccc ggcagacgca aagtccgcgc ccgaagcaaa ataagccgca tcctgaggca
     3121 ctttcgcatc atccgcacca tccgcatacg caaagccgac atcggcagtg ataccggaag
     3181 acatcgccgt cttcacaata atctgcgcgt catccaaacg gaaaccctgt tccaagacac
     3241 ccagcgccaa cacatcgccc gccttaggcg gctcagaagc atccgccccc aagatcgcac
     3301 cgtcctgctt cgcaacgata cggtaatgct ccgccgccag attgccgtaa ggcacgccgc
     3361 cgaaacggtt gccgccattg tttttcacat aaatctcagc cataaaaaac ctcaacccaa
     3421 caaataggaa aagccgccct ttcaagcggc attcgaatca gcggataccg ccgtgcaaag
     3481 gaaccaccgt atccacaacc gtgacaccgt agtcggtaat ttccttcgcg cccccgccga
     3541 catccacggc aaagcgcgtt ttcgccacac cgcgtatcgt accgacgagc gtttccacac
     3601 ggttgccgtg gtcaagctcc ttctcagacc aaaagaaagg cataccgccg tgtttgccgg
     3661 tattcgcaaa cgcctccaac accgcctggc cgcccaaaat aaccgaacgg tcgaccgcaa
     3721 acttgtccgc aaacgaagcc gggattttca agcccgattc cgcctcgctg aacttatcgg
     3781 cgcaatactt catctcatcg cccgcataga aacggatggg gcgcggcatt ttcaccagga
     3841 taaagccgtt ccacaaaccc gcatcgccca aaaacagcgg attttgattt gcctgggagg
     3901 cgcgcgcgat tgccgaagcc tgaagctggc ggaatttcgg atcggcggca aaacggttgt
     3961 actgcgccgg ggaaagcagc cacacgcgca aaggcgaatc acccgccgcc ttgtcgccct
     4021 caaacttcac aatcggcggc ggcagcggaa tctggtcgag cacggtacgc atactgtcca
     4081 ccgtatccat cgtaaacaaa tcggtagagg caatcttcaa ctcgccgccg ttgtccccaa
     4141 cgcccgttac cgcatcgccc gaagccgtaa aatggcggtt tttggacggg gctttgaccg
     4201 gattgaccgc ataatcattg aatttcgggt cgcccgccaa aggcacgccc cattcgatat
     4261 tgtcatgaaa accgcgcgcg cccgccatat gcaccaacag cgtctggtcg gcataacggt
     4321 ccatcaggct ttgcgccgcc ggacgaatca gcgcgcgata atcggcaggg ctgcgctgat
     4381 tcgtcatcgt gccgccgccg tcaacgggga aacgcgcctg attcacacgc aaacccgcct
     4441 ccgacaggct catccccacg cccctgcctt ccgccgtgtt gtcgcccata atcggcaggg
     4501 cggaaacagg gttgaccaaa ttgaaacgga tttcgtcacc catgccgcgc gtcaaatcct
     4561 gacagcgcac gaccggcata tgctgggtcg tctgtttgcg caaagtcgcc tccgcacccg
     4621 cggtaccggc aggcatcttg cccgccaaac ggttcagcgt actgttgcgc tgcatatgca
     4681 tcgcaaacag ccccgccgcc tgcttcatca tcgcctgcgg gtcgccgtag gccgtattcg
     4741 tcttttgtgc cataaagagc ctccctcaaa aactcaagaa tttaaaaact tctcaacctg
     4801 ctcttccgtc atggaagcca gtttttccgc cagcgcgttg ccgctcaaat aatccggcgg
     4861 gccgtccgaa gccgtatggt cgcgccccgc cggaatatcc gacagcgtat tgggcgtatt
     4921 cttcccggca ggcggctttt cggcgggctt ttccggttcg gcggcggatt ttgccgcctt
     4981 aaacatatcg aacgccccga tgacctcggc ggcagtcccg tccttaagcg cgtcattaaa
     5041 cgccttgcgg acaagcgggt tctgcgcatc cagccactcc tccagctcgc ggctttccac
     5101 aatcgaatcc gcatcggggt gcgccgtata gatttcgcgg taatgcgcct ttgccgcctc
     5161 ctgccgcctc ctgccgcttc atgtccgcct cataacccgc cagcttctcc tgaatcagct
     5221 tttccacgcc ctttttcaca tcctcgtcgg aaaaatcccc gaacaacgaa ttatcggaac
     5281 cgtcagccgt ttccactccg ggtttttccg cccccttctc aagctgggca atgcgctccc
     5341 tcaaggcttg ggcttcggct ttcgccgact tttccgcctc gcgcgcctct accagctttt
     5401 cataagggat agtgtgtttg ccgtccttgg cacggatgac cttgtccgca cgccgtccgc
     5461 cgcgccgata cccgcgccga ccaccgcgcc cgtactgccg accaccagcg attccgccca
     5521 cttcttaccc tccaaaccgt cttggatatc ggggaaaacc aactggtcca aacccaaacg
     5581 ccccgacacg tccagcggcg tcaggcggct gaagcccttg gccaacacct cgcccgcctt
     5641 atccccgaaa gtgtccgcca acatattgcg caacgccgct tccgcatccc acgggtcatc
     5701 gtcgtcactg cccaacatcg aagccaccgc aagcagcgtc gacacaaacg gcagccccaa
     5761 gatgcccgaa gccatcgcat gcgagaccaa cagccccgcc agcgtcttac gcgcctcctt
     5821 atccccctta aacgcaaggt acgcattgcg ccccagcgca tacaggatat tctgcgaata
     5881 ctgcttgaac aggaagacca ccttcgccgc attgcccatc ataaagcgcg gacggttttg
     5941 cgccgcatag tcaaaatgcc cgtcgtacgt cgcctttttc gcctgttcga aagccgcctc
     6001 actgtccgcc cccgcccgtt tcgccaaacg gtaggccgcg acaaacgtaa cctggcggtt
     6061 aaacttctcc gcatgatgga acagccaggc cgccttatcc atcaccttct gccaagccga
     6121 ccccgccaac cccgggtcgc cgttggccac ccccgccaaa tcatgcgcct gcgacaaatc
     6181 gatcacaccg taatccgccg cctttcggaa cgccgccttc tcatccccgt tcaaactgtc
     6241 ctcaatcgta ttgaacttct ccccgaacct cagcccgatc tgttttgaag cccgcagcaa
     6301 ttcccgcgcc gcaccggcat aaccccactt cgccgccatc accggataag ccaccattgc
     6361 cgtctgcgac aggttcacaa ccgcagaagc gggcgacatc cccatcatcc acaaaaagcc
     6421 gaagcccgtc aaagcctgcg ccagcttgga aggattcgga ttcatcaccg cctcgtggcg
     6481 tttcctcatc tcatccgcca cacgctgcaa ctgacgctgg tcgaaaccct cctcatattt
     6541 gcgcccgtcc acaaaatcct gcatcacatc caactgttcc gccatacggt ccgcatagcg
     6601 caacttcgcc agataattcg caccgctgcc catattctgc gcatatgcgc gcctcgcatc
     6661 atcgctgaag cccggcacgc ccttgcggtg gatgccgtgt ttcgcccaag acgtatcggg
     6721 cagggagttc aaataaagct gcgtcagcgt atcgttcaat cgggcccgtt gcgccggatc
     6781 caaatccatc ctgccgacag cctcgcccag ctccttcata aatccgctgc cgaccgcatc
     6841 gcggcttcgg atataatccc gggacttcat cacgggcgaa accttaaacc ccggcccgaa
     6901 atcagccttc agcgcatcgc gcagcttctc cgcctcgctc aaagtttccg cgcgggacac
     6961 attcgcacta ttcccgtccg catccttgac caccaccaga taatcgccga aacgcgccaa
     7021 agggaaatac acaccgccca gctcgttgtc aaaacgctcc ttcaggcggc gcaccgtttc
     7081 cgcatcctgc cccgcacgcg ccaaccgttc ggcaagcgca tcgcgcacct gcccgaaatg
     7141 cgccctataa tcatcccgcg ccttgcggta caccctctgc gccgcatcag gcagcccggc
     7201 aaaacggcgg ttcagcaaag caagcccctc caaagccttt tgcgccccaa cctcatcctt
     7261 ttccaaacgc ttcacatccg cgcgcgcctg ctccaacatc cgtaccgcat ccgttttagc
     7321 ccggctttcc gccgccgcat ccgccccggc gcgcctcaac gcccgcttcg cctccgaatc
     7381 cgcataaaac agacggcgca ggcgcatatc cgcctcatcc gccaaaattt cccgaccgtg
     7441 tttttcctgc gccttctcca gcgcataagc cgccttatcc gccgcccgtt gcgccttatt
     7501 gtaagcagca tccgcacgcg cggtacgcgc accggcggaa gcaacggccg cccgcgcctt
     7561 cgcgatttta ccgtccgcaa tatccaaagc cgtccggatg ccgtccaaac gcccccgggc
     7621 atcctttcgc atcagcgggt cggcatccac cttcgccagc gtcgcatcgt gcatcagatc
     7681 cgcaagcgct ttcgcatcct cctccttcaa cctgccccat tccctgacaa ccttgtccgc
     7741 ctccgtaacc gccttgttcg catccgcgcc gaagagttcc gacaaacgcc cgtattcctt
     7801 caggccgtcc aaaacgccgc cgtaaatgtc ctcgatctgc cgcctgccca aaaactggag
     7861 ctgaatcccc ttggaagcat cccagcggtc tttcagcgcg tttctcaaat cggatttttt
     7921 acccgttata cggctgattg cggccccgat gttcgccgca cgggaaaaac gcaccgcctc
     7981 cgtgtcaaaa tcggcaatgc cgcccttgcc cgcatctttg tcttgcaaaa tctgcaacag
     8041 gcgtttatca tcaatgcccg ataaaggcga tttgctttca gaggctcccg tagttgatac
     8101 gcggtcaagt aatgaaagca agttgccttt ttctattttc gtcagcttat ggtcgtaata
     8161 gcggttaccg ttcctgctaa ccgcgacggc agcccttacc gtgtaatccg caccgccgac
     8221 attcagtccc gaaacataat attcataacc ctgaatatcg ccgttcttag ccaaatcctc
     8281 gttcggcagc gtatcgatat acaccgcatt ctcaataatc tgcggaattg ccgcgatact
     8341 ctgcaaatgt tccgcgtcct tatagtcgtg acgcaatatt tcagtgatgc ccgaacgtcc
     8401 caaactgatt tcacgccccg tgtccttatt cacataaggc ccgcgcaaag atttgccgta
     8461 ttccagcgca ttgcgcttat attcgcgcaa attgccgcct tccggaacct ccctgcccga
     8521 gatacggatg gtttccgcac ggcgcagctt ctcaaggctt tccgacttgg aacgttccgc
     8581 cgcaccgtct tcagcccgcg cgaacatcac tgcacgatga ttgccgtccc aaggcgcatc
     8641 ccgcgcccct tccacattcc cgtgcaaatc cgccagcatc gcaaacacat ccgcgtccga
     8701 catcgcaccg ccggccttgc ccgccaggcg ttgcagcaca cggcgcaaca gggcgccgat
     8761 acgcgccaac acgcccgcaa tgccgcgttt caaaccgttg ccgaccttca cgccgtaacg
     8821 gttttcaatg cccgcccaac cgccggtacg ctgcgccgca taaagctccg cgaccgcctc
     8881 ttccaccgcg gcggagcgca ccgaggccgc cgcatcgccc gtgccttcgc gcccttcctg
     8941 caccgcgtcc gcaatgcgcc gtatcaggcc gttgccgtcc gcacgttcca attcttcacg
     9001 gtacttggcg aaaccatccg ccgcaaagcc cctgtgcccc agctcgtgcc acgccgccca
     9061 taccgcgcgt tcgggcgtaa ggttttccgc caccagcgtg attttgcccg tcctgccgtc
     9121 aaaccagccc tccacacgct gcgacaacag catctgcgcc ttgtccggcg ccgtttcgga
     9181 cacggcggcg acatccaccc gttccgccag tccgcctatg ctttcggcga ggcgtttttt
     9241 gatattttcg gcgtgctcgc gcaatgcctt aaaaccttgc ggcgcctgac gctctttcac
     9301 cgtataaaca ggccctccct gcaacacacg cgccttgcgc cgtgcctctt ccggattgtc
     9361 cgaagccgtt ttcgcaaacg tttcaggctt ggcagcctta tccgaagacg gcttttcggt
     9421 tgccgcgcct tgtgcggcgg ccgacttgcc ctttccgcca tcgaagtggg attttcccga
     9481 ttcgatattt cgcaccttat ccgaagacgg cttttcggtt gccgcatttt ttgcagcagc
     9541 cgccttgccc gcaacaggcg tatcttttgc ccgggcaggc tgcgccctgc ccgccggttt
     9601 gccgtcggcg gcggcatcgc gcggccgcgc catcaccctg gccggcgccc ttgcttcctg
     9661 cgtcggacga taatcccggc ccggctcgga cggcacggct tccgacaaac cgcccgcccg
     9721 caaggtttcc gcctcccgcc ccttcaaata acggcggccg tcggagacaa ggccgtcaga
     9781 gttggaagga ccggccatat cggcaaactc ctccggcatc ccgcctttgc ccggcctgcc
     9841 cggcacgccc gcaagcgcat caatatgcct gcggtccgta tgctcgaaac ggtattcgcc
     9901 gccccccaaa ggcacacccg ccttttccgg cctgcgcctg tccgccgccg catccgccat
     9961 ccgtttctta ggcgataccc tgcccgccga agccgcaacc tgcggattca ataccggcaa
    10021 caccgtatcg gggcgtttgc ccgcaacagg cgcacgtttc ccgccgtccg tcccggcaag
    10081 gttttcagac gcggacaggc cgggcgcggc accttccgcg cgcgcccgaa cctgtaagtt
    10141 ttgccgacgg gccggcgcat tgccggacgc aacggcggac cctggcaaac cctgcaaggc
    10201 agccgcattt gcatccgccc ccgcatcagg ggtcaaaccc ctgccgcctt gggtcaaacc
    10261 ctgccgcaca tcctgccgca catcctgcgc caaaccctta tcttccgtgg gtaaagcccg
    10321 ggtcaaaccc ctgccgccgc cgacaacggc ctcagacgcg ccgtcatccg gcaacgccgt
    10381 ccgaaccccc tgcaaacgct ccccctcgcg gctcaacagc ggcacgctcc aaccgttgtt
    10441 gcggcggtaa ccgtaaaccg cacggttaag cctgtccaac acatccgccg attccggatg
    10501 ttgcgcacgg taagcgcgta cagccccggc cgattccgcc aaaccctcag gataatgtcc
    10561 gcgcatatcc gccatacccg cctcccggcg cataatcccg tccgccgtat agtccgccac
    10621 cgtctgcgca tgacggcgca cccattcagg cagcgcgtca aaccgcctgc gcgattcctt
    10681 atccaacgca tcggcaggga cggaaaaccc ttttttagga acaggcacgg catccgccat
    10741 acgcacgggc ggcggcaggt aaccctgttt ggcttcggaa gccgcctgat aatccgcctc
    10801 aatccgcgcg tgatacgcct gctcgatttt tcggcgcaca ggcgcggcga tttgcccgaa
    10861 atccgcaacg cccttaatat caaggcccgc ctccttcgcc aaagccttgc gcccccgggt
    10921 atccaagccg tcgaaatatt ggcccgcagg cagcggcgag acccgcgcaa tcccgcccga
    10981 agcggcggcg gcagaaccgc ccggcgcaac cgaagccgca ccgcgcggcg caccgccggg
    11041 gcgcacattg ccgtaagcgg acggaacacc gcccctgtcc gcatccgccg accgtccgga
    11101 cggcgcatca tccgcaaccg accgggcttc catatcgtca gacaccgcag acggcacagc
    11161 cgcgcccgaa tccgccgcca aagcagcggc cgcagaaacc gcaccgtcat ccggatcaat
    11221 ccccaccgcc tccgacggct tggcccggta tttagccgcc atgtccgcat actgacgcgc
    11281 caaaccgtcc ggcatcacgc ccgtatccaa aaattccgcc ccgtcctccg acgggatacc
    11341 cgaaacatcc ggctgttttt ccgtcccgcg cgccaattcc tccgcgtgcg gcgtattgcc
    11401 gtcggcaggg ccgctgaaaa accgccccgc ctcctcctgc ttcgtacggt ccgcaaactg
    11461 ccgcaaagcc ggatgcgact gatcgtgatg cgccgtatca aaacccgcgc cgccctgttc
    11521 cgcgccgccg tccgcagccg cctgtgccgc cgcacgcaac gcctgcgccc cttccggggc
    11581 cggcctgccc gccttcctgt tgcccccgat acccccctgc gcacccgcct tccgtccggc
    11641 cggcggcctg agcgggtcag ttaccgccgc ataagccttc gttccggcaa accccaccgc
    11701 agaatgcccc aaagaagaga aagcctccaa agccgccccc ttctcgtccc attccccgtt
    11761 tgccagcccc gtacccaaat actcgcccgc aaactcaccg gccgattccg tcgcataagc
    11821 cgccgcgtgc cgtgccgcac cgcccaaacc gcccttggca gactccttcg ccagcgccgc
    11881 aaattcaggc gttcccttag ccgccgcaac cgccgcctta tccgccgccg caacacccat
    11941 tttctccaat gcggctttcc cggcggcctt cttgcccata ttcaaaatac tgccgccaag
    12001 tttcatcgcc gccatatccg ccgcgccgac caccgcgccc ttgaccgccg catttttcaa
    12061 cgcacccggc gcacctcggc cgataaacgc catcaccgca tccttgtccg cagggtcgac
    12121 gcccgccgcc tctgccgccc tgtccagctg cccgccgtat tccatcagcg tattgcccaa
    12181 ggtcgcgccc gcataggcgc cgacagccgc gttaccgccc gccaaagcgc ccaatatgcc
    12241 gccggcggtt gtcgtagcaa tagggacaag cgcattgggc gcctgctcca gcaccgcgcc
    12301 ccccatctcg cccacatctt ccaaggcgcc gtccaaaccc ttctcgcgcc agtccttctt
    12361 aagctccccc cacacattcg acaggccgcc gccgacaccc ccgccctttt cccaagcctc
    12421 gcccagctcc ctgcgcgctt gggatttgcg cccctcctga atcttccgat aatccatatc
    12481 ctcggccttg agccggccca gttcctcttt gtcgcccgta agggcattcc accccatacg
    12541 cgccgaacgc atagaatttt taaacccgcg cgtcaacgcc gtatcgtcgg cggccgcccg
    12601 cgcatccttt tgcgtaaagc cgcggtactc cttcaaaccc ccggcaagcc gcccgtgttc
    12661 caacggatcg tatctgacca aatcactcat tgctcaaccc tcttctcacc cttctcattc
    12721 tcataaatga ttttaccgcc ggccgttttc tgaccgttcg gcttaaagcc caactgcgca
    12781 taacgcttct cgcgctctcc cgcaaactcc acatccaaag gcttgcccgt ccgcaaatcg
    12841 acaatcccct cgcgcttcgc cgtcaagccc gtatccgggt cggcaacctc ataactgata
    12901 gtcttgaaca cattcgggtc aaagcccttc tccccttgcc gctcaaactt cccgttaagc
    12961 gcatcaatct gccgcgcaat ctccccgcgc tgcccgtccg acttcgcaaa accataagcc
    13021 cgcgtcaaat cggcgatgcg cttcctatcc tcaagctcca gcgcatccgc ctcacgcttc
    13081 atctcaaacc cctgccgctt catccccagc tcggcctgct gcacatcaaa cgccctgtcc
    13141 gcattcctct gcgccatctc gcggttcaac gcaccctcgt tcgccttaac cgccgcatca
    13201 aaccgcccct cctgcaacac cgcctcacgg ttcgccgcat tctccgccgc agccgccgcc
    13261 agcctttccc tgcccaattc cctgaccgcc gcatttttat tatgttccgc cacaatcccg
    13321 cgcgccgcat taagctgcgc cgccgtcaac tgaccgttca aggcccccgc atgaggcgta
    13381 gtcgccctcc tgtaaagctc ccgctcctca ggcgacagcg catccggccc ggcagacacc
    13441 aaaggacgct gcgcgtcaaa atcacgcacc ggtttgccgt cccgatacac attcccgtca
    13501 aatcccaaag gcggcggcgt ccgtccgccc gcatccgtcg ggcgcgtatc gcgccgcgcg
    13561 aaatccgccc cggcccgcgc atcgggggaa agcgccttat cgccgcccga aaaccccaaa
    13621 ccggcatccg caccccgttc gaaagcaccc gcccggcgca accgccgcgc cccatcctca
    13681 atattgttac ggctcaaatc catcacattc cgataacgcg ccggcccggc ctcataattc
    13741 gcaacggtac gccgcaccgc atcggcacgg tgcgcctgtg ccgccgcccg ctcccgctcc
    13801 tccgccccct tccgggcctt ctcttttttc tccttttcct tcatctccca aaaatccccg
    13861 gcagacggct caaacaaacc catcgcaaac cctccgaaca aataaagccc aaaataagca
    13921 aaaacccaaa cccaatcaaa cgcctaaagt taagaacaaa caaaaatcgc aaccacaaac
    13981 caattaataa acaacaagtt acacaaaaaa acacaccgac gggccatttc cgggggcaaa
    14041 taaaaaccgc cttcgtgcaa ggcggttgca aaaaagcctt tcatttaacg aaaggctttt
    14101 tgccgcccgc gtaccgcgtt cagaggttgt ccgacaccgt cggcgtttcc atgcctgttt
    14161 gggcgtgatt cgcactcttt ggcggcacgg ccgtcatcgg gccggtatcc gcgccgtagg
    14221 cttcagggat gccttcgggc ggtatttgcg tttcaggcgg catggcgggg acggggaagc
    14281 cgggatcgtc gccccgcgcc gccctgatgc cttcttcgat accggtcgcg ttttgcacga
    14341 aaaccgactt gcgccccagt ccggtcaaaa tctccatcgt acttttgcct gtttggaagt
    14401 tgcgcgtcct gccgtcgtgc ggcaggaagt ccgtccccca ccggtagggc agcttttcca
    14461 attcggcaac ataccagtcc aacgtccggt gcgtgtcttc gatgtagccg atgatgcgca
    14521 cgctcgtcaa atcgcgctgc accagcccga tggtcatggc atcgttccag cccaaatccc
    14581 aaaccgtatg cacgggcaaa gaagaatcat aagggacgag tgtaacgcgt ccggaatgaa
    14641 aagcgtcctg tatttcatgc cggtaaaccg ccccctccga taccatgcgc ggcctgcctt
    14701 cccaaatatt cccgtagtcc tctttattca tcgaacgctg tgctttgagc cgctcccggt
    14761 tcaatgcttc gggaaaccac ggattgtcgc gccagttgat ttcgcacaac caagtgtctt
    14821 cggacggcat agcgataaaa cgccggtagg tttcgtccgt ctccatatcg ggattgaggg
    14881 taatccaaat ttccgaacct tctttgcgta tggtcggcgt gagcacgtcc cagctttttt
    14941 tactgacgcc gtgcccttcc tcaacccata cgatgtcgat accttcaaac gatttgatgc
    15001 tgtccacggt atgcgactgc aggcccgaaa acacgaacag cgtgccgttt gcgccgcgta
    15061 tctcgaagtc ggttatttcg tagaaatgcc ccaaacccaa ctgcgccact ttgtctttca
    15121 acaggcggtg tacggtatcg cgcatcgatt tttgaatttc gcgcacgcat aaaatacgca
    15181 aagggcgttg cgcccccagc gccagcagtg cggatgccgc gccgtgcgat ttgccgccgc
    15241 cgcgcccgcc gtacataacc ttgtaccggc aaggcttgaa cagcccgtcc agttttgcgg
    15301 gcaatttcaa atcaaccgtc tttcccgtca tcttcaggcc ctacaaattc gatacggatg
    15361 cccgccgtct tggcggattc cgaaatcttc gattccgcgg tatccatacc gtatgattta
    15421 cgctcgattt ccaccaaaac cttaaacccc gccgccaccg tttggaacat acgcgccttt
    15481 gtgaatgtgt cctccgggct tccgtacaat tccgcgacaa tctgctccgc gcttttggac
    15541 aggttttccg ccaaattccg atgcctgcgc tgtacggcgg caacccgttt cgcttccgcg
    15601 tccgttactt gtttaaggtt gaaatccgca taatccggcg cattgccgtt tttgtccgcg
    15661 gacagcacca tcgcccgcgc ctttgcctgt acctcgtcat tcaaatcgcg cgtccacacg
    15721 cgttctttgg caaccctgcc gacgtgttgc gccgaaacat tgtatttgcg gccgatttcg
    15781 gcaatgctca aaaccccgcg ccggtaatcc agctcgactt tgcgccaatc ttcatctgac
    15841 aatctcggca ttaaaggtcc aacctcccgt ttttggcgga acattccaga taattccgca
    15901 atactgaaaa ctcgcgcccc atctgaatac tggaaaactg ctgcccgaac cccgacatca
    15961 aagcccccaa cgagcgcatt tcctgcgggt tgaggtgcaa taccgtatcg cctatttcca
    16021 atacgcccat ccccgaatcc aataccgata cgctgacggg gcgcgtctcg ccaaacccta
    16081 tggcgggctt gaaaatcccc cttgtcacgc ggtagatttt gccgtgctcg accagcagct
    16141 tggtaaattt agtgatttcc gacattttca ggcctgtcgt ttccgccaaa gcctcgcggc
    16201 tgaccacctg ctccgcgttg tgcaaatcca caaccccctg gtacaccttg tcaatatccg
    16261 acaatcccga catcaaatca aactcctaaa catcccaaac gattccaaac tccgccgccg
    16321 cccgcgattg caggcggtta cactacaaag ccaaaatctt tagatgctgc ctctctcgct
    16381 ttcactgcat cttcaatatg ttcaaataaa cctaatattt tattttttct attaaccata
    16441 atcctagctt gccatttctt agacggctta tggaaataaa ccccagtatg cccgctagta
    16501 ttatttttaa acttccctct atttttcata ttttcagcat gtgtaacatc tctcaaatta
    16561 gatattctat tatctgtctt atccctattg atatggtcta ttgaagaagc gtgtttccca
    16621 taaacaaaaa gccaaattaa tctatgtgca ttatattgtt tcttaaatat ttttaacttc
    16681 aaatacccca acctgccttt gtataagaaa acctccttgc cggaaaatcc ggtattccaa
    16741 gatttgtaat gcaaaccgct attgaaatac tttcttggac gctccgccca atataacttt
    16801 cccgtattgt catcatatct taataattct tttaaaacct cttgtgttaa tcccataaaa
    16861 caccatgctc catagatgcc caagatataa tcttttcttg atattcagac atttcccgaa
    16921 ctgataattt tgtcgttgat atacccatta attgcccgtt aggcatttca atacatccaa
    16981 taaattttct ttttaaaaat tcatgccata catcttgact aaaccttctc ccatcgataa
    17041 aaactttttc tgaaatttca cgatacaaaa accatagccg cctgttctgc tcgacgctcc
    17101 gttttgactt gcgcgggcgg attgtgattt caaggttgcc gttttcaaac cacccgtccg
    17161 ggctgtccca aatcgaccgc ataacctgct ttttgttttg gggcgtcagt gtaaattccg
    17221 ccccgttcat ttcagacggc ctttcacgcc gacaaccccc aatgcttcaa ggcggcgtat
    17281 cgtgcgcaat tgcgaacggc gcatataaaa ctctttgtct tcgcggctta ggcctgcgcc
    17341 cctgccgtcg atcgcgccgc ggcggcaacc gcacccgaaa cccgcgccca aaccgccgct
    17401 tttcaatccc gccccgtgcg tctcgcccgg gaaacggcac aacacgaccg tttcagggtt
    17461 gtcattgcac acgcccgcga tgttcggcgt gcattgcccg cctttggcgg ctttgcgtat
    17521 cgcgctagac atcccaaccc tcctgtccga tgaaatccca accctcgtct gccgcatccg
    17581 tctgttccat
//
LOCUS       DAWTCI010000044        16537 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWTCI010000044 DAWTCI010000000
VERSION     DAWTCI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.044"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            175..2202
                     /locus_tag="WM481_001374"
     CDS             175..2202
                     /locus_tag="WM481_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFA9080678.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSGLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFGVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(2291..3844)
                     /locus_tag="WM481_001375"
     CDS             complement(2291..3844)
                     /locus_tag="WM481_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFA9080679.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFENVRRFPEKPDL
                     DRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGEDEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(4096..5289)
                     /locus_tag="WM481_001376"
     CDS             complement(4096..5289)
                     /locus_tag="WM481_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFA9080680.1"
                     /translation="MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDALRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDG
                     KTGKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLAIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVLLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAQGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            complement(5286..6269)
                     /locus_tag="WM481_001377"
     CDS             complement(5286..6269)
                     /locus_tag="WM481_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HFA9080681.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGT
                     EWKQGTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKVKLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAEKIMEKIVFEPSTQPSSTQP"
     gene            complement(6280..7716)
                     /locus_tag="WM481_001378"
     CDS             complement(6280..7716)
                     /locus_tag="WM481_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFA9080682.1"
                     /translation="MPLLSVEFALFFIVFLPIYWGFAKYPSVQNLLLLAAGMGWLYHI
                     SPVFAAIIVLYSSCVYLLGELLRSDRESTRRFWLGCGIAASITVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSVAYLVYCFRAPHAARFGWHELLLHLSFFPTVTS
                     GPIIRAAAFKSTDGEQAGALAQIRTRRPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALALLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANAGGWNAPQRADMLLLASFASLMLLYPYLQRAFDGTVKGLEKIPMWL
                     WFIQISIILLLIIVLAPSGIPGFIYANF"
     gene            complement(7846..9011)
                     /locus_tag="WM481_001379"
                     /pseudo
     CDS             complement(7846..9011)
                     /locus_tag="WM481_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            9452..9664
                     /locus_tag="WM481_001380"
     CDS             9452..9664
                     /locus_tag="WM481_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFA9080683.1"
                     /translation="METLLLDIVGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTAAEALIEAVEDGGYDAALK"
     gene            9864..11390
                     /locus_tag="WM481_001381"
     CDS             9864..11390
                     /locus_tag="WM481_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFA9080684.1"
                     /translation="MPGVSDIKNSYDDLMYESKAFSQTAINALEACARMMGLKPTAAA
                     KAKVLELGCSMGRNIITQALYYPDAEFVGIDLSGRQVAQGNAIIEKMGLENVRLEEKD
                     ILTVDESFGKFDYIIVHGIWSRVPDAVKDKIFSICRNNLTEYGIAYISYNVYPGWKRQ
                     EQLRDIMQFAGRDALGEPLEARTRKGLDAIKALAEILENDKGLGGGKLPAIQKILNHN
                     TYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLHVSFVSWMAEHTRERILALA
                     GGDYIAKEFYSDILSDRQFHRSLLCREEVGDTVRRDESVAVEVIESLNFRPARGETIN
                     FDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLEFDRMKINSQLLLQTILGRFS
                     VSSDNAGKPFFEDHKTYVPARFTNYAAAFVEHGAGAFVRPANRYNESTPSFGYGHLYI
                     MRQLSRPTSKQALIETVAENLNIVSATPDGLTFHPPAEVYVEEILADLADRHFLVSAD
                     "
     gene            11472..12569
                     /locus_tag="WM481_001382"
     CDS             11472..12569
                     /locus_tag="WM481_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFA9080685.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYVLGFGSGKVQAALGQNLLQAGLPLMQ
                     VWQENLASEGITLFANPLSPDTPARALSDGSHTRQRMAMDVFAANAIRAVRMQGPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFCWQLSFTDNIAVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGRNPFFSA"
     gene            12683..13797
                     /locus_tag="WM481_001383"
                     /pseudo
     CDS             12683..13797
                     /locus_tag="WM481_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            13922..14377
                     /locus_tag="WM481_001384"
     CDS             13922..14377
                     /locus_tag="WM481_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HFA9080686.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLETDSAGTSG
                     WHDGEDMHRETAKILKKYGIDASGFTSRKIRQSDATAFDYIIAMDGKNLSELEKTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYMGDFEETYRLADAGCRALLEKISK"
     gene            14454..14930
                     /locus_tag="WM481_001385"
     CDS             14454..14930
                     /locus_tag="WM481_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFA9080687.1"
                     /translation="MKTNFKQKIIEQARREDLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWADQGVLHKVAAVNGYILCSHAQHECNDHCHDHEEA
                     EAHHSAFILVCTECGAADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            14918..15844
                     /locus_tag="WM481_001386"
     CDS             14918..15844
                     /locus_tag="WM481_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFA9080688.1"
                     /translation="MSEVKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAAGKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVVEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            15909..16466
                     /locus_tag="WM481_001387"
     CDS             15909..16466
                     /locus_tag="WM481_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFA9080689.1"
                     /translation="MKPKFKTVLTALLLAVCLPSMAATRVLMETDMGNIRLVLDESKA
                     SKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLVQKATDKAVANESGNGLKN
                     TVGTIAMARTADPDSAAAQFFINLADNGSLDYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATLGLYQNVPVQPVKIRRVVVGQ"
BASE COUNT         3873 a         4182 c         4671 g         3811 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
       61 catttcggcc ggtttgtgca tttcgtcatc cggaagccgg tattttgccg gcaggttttg
      121 actactttgc ccgatgaccg ggtttgtctg tttttaacaa aggagtgaga aggtttgtcc
      181 ctgtctgaat ttatagaacg ccgaacgtca tttaatccga tggttatttt gacgactttg
      241 ttttttgtgt gtgttttggt ggtattggtt ttaaccgtgc cggatcaggt gcagatgtgg
      301 ctcgaccggg caaaagaagt catttttacc gagttcagct ggttttatgt tttaacgttt
      361 tccatttttc tgggttttct gctgatactc tcggtcagcg gtttgggaaa catcaggcta
      421 ggacgggatg aagatgtgcc ggaattcggc ttcctgtcgt ggctggcgat gctgtttgcg
      481 gccgggatgg gcgtgggcct gatgtttttc ggcgtggcag agccgttgat gcattatttt
      541 tcggacatta cggtcggcgc gccggaacac aggcagcagc aggcattgct gcacacggtg
      601 ttccattggg gcgttcacgc ctggtcggtg tacggtacga ttgcattggc tttggcttat
      661 ttcggtttcc gctacaaact gccgcttgcc ctgcgttctt gtttttaccc cctgttgaaa
      721 gaaaaaattt ccggaaggtt cggcgatgcc attgatatta tggcgttgct tgctactttt
      781 ttcggcatca tcaccacatt ggggttcggg gcttcgcaac tgggcgccgg attgcaggaa
      841 atgggctgga ttgccgaaaa cagcttcggc gtgcaggtct tgattatcgc cgccgtaatg
      901 tccctcgccg tcgtttcggc aatatccggc gtggggaagg gcgtgaaggt gttgagcgag
      961 ttgaacctgg gccttgcgtt tttgctgctg ttttttgttt tggcggcggg ccccactgtt
     1021 tacctgttgt cggcattcgg cgacaacata gggaactacc tcggaaatct ggtgcgcctc
     1081 agtttgaaaa cttatgcgta cgaacgggaa cacaagccgt ggtttgaatc ttggacggtg
     1141 ctttattggg cgtggtggtg ttcttgggcg ccgtttgtgg gtttgtttat cgcgcgcatt
     1201 tcaaaggggc gcaccatccg cgagtttgtc ttcggggttt tgctcatccc cggcctgttc
     1261 ggcgttttgt ggtttaccgt cttcggcaat acggcgattt ggctgaatga cggggttgcg
     1321 gggggaatgc tcgaaaagat gacctcctct ccggaaacgc tgctttttaa attctttaat
     1381 tacctccccc tgcccgaact gacgagcatc gtcagcctgc tggtcatttc cctgtttttt
     1441 gtaacttctg ccgactccgg gatttatgtc ctgaacaata ttacctctcg ggacaaaggc
     1501 ttgagcgcgc cacggtggca ggcggttatg tggggcgtgc tgatgtctgc cgttgccgtt
     1561 ttgctgatgc gctcgggcgg actcggcaac ctgcagtcta tgaccctgat tgtttccctg
     1621 ccgtttgccc tgctgatgct gataatgtgt ttcagcctgt ggaaaggctt gagtgcggat
     1681 aagaaatatt ttgagacccg ggtcaaccct accagtgtat tttggacggg cggcaagtgg
     1741 aaagaacggc tggtgcggat aatgagccag acgcaggagc aggatatttt aaaattcctc
     1801 aaacataccg catcgcccgc tatgcacgag ttgcaacggg agctttcgga agaatacggc
     1861 ttgagcgtcc gggtcgataa gatgtttcat caggacgagc ccgcaatcga gttcgtcatt
     1921 cggaaagaga cgatgcgcga ttttatgtac gggattaagt ctgtcgggca ggatgtatcc
     1981 gaccagttga ttaacgacgg caagctgccg catatccggc accagacaac ttacaaaccc
     2041 tacgcttatt ttttcgacgg gcgcgtcggg tacgatgtgc agtatatgaa caaggacgag
     2101 ctgattgccg acattttgaa aaactacgaa cgttatttga tgttgttgga tgatgtcggt
     2161 caggaactga tggcgcacga gcaggtggaa ttggcagagt aagcgccttc cctccggcgt
     2221 ttgccaaaaa atgcggtgaa tcagattcac cgcatttttt tgccggacgg ggcttcggac
     2281 ggcacggcgt tcatttgttt ccgtcaaact gctccttcag cacccgtttc aatactttgc
     2341 ccgtagcgtt gcgcggcagc ccgtctttaa agtggatctg tttggggatt ttgaaatttg
     2401 ccagcacggt acgcaggtgg cggcggattt cgtcctcgcc caaatccata ccttccttca
     2461 attggacgaa ggcgacgatt tcctcgtcgg cataacggtc tttcacgccg atgacggcgg
     2521 cggcttcgac ggcatcgagt ttgtagattt cttcttcgat ctcgcgcgga tagacgtttt
     2581 gacctttgga aataatcaaa tcttttttgc ggtcgacgat aaagataaag ccgtcctcgt
     2641 ctatggtaac gaaatcgccc gttttcaacc agccgttgac gatggtttca tcggtggcgg
     2701 caggcatatt gaggtagccc cgcatcaccg aaccgcccct gacgatcagt tcgcccactt
     2761 cgccgcgcgg cacttcgacc aattcttcat cgacggcttt ggcttccaaa ccgggcaggg
     2821 ggatgccgac gctgcgggct ttttgccgtt cgggcgtatt gacggcgacg acgggcgagg
     2881 cttcgctcag tccgtagcct tccagcaatt tggcgcgggg gaacttggct ttaaaatcga
     2941 ggatggtttg ttccgccaaa ggcgcgccgc cgctgataaa caggcggatg cggttgaacc
     3001 atctgaaata ccaagggatt ttcgccttgc tcatcgcggt gtaaatcgcg ggtacgccca
     3061 aaaacacggt tgcgcgtttg agcagggcct gtttcaaaac gttggaaaag gggaaaacgg
     3121 atttgaccaa aataatcgaa cacgccatat aaatcggcag cagtaccata gccgtcagcg
     3181 tgaagctgtg gaacatcggc aggaaaacga taaagcggtc gcgtttggaa attttaaaga
     3241 tgcgttcgat gccgttcagg ttggcgaaca ggttggcgta actgattagc gcgcctttgg
     3301 gatgccccgt cgtgccggag gtgtagatga tgtgtgccaa atcatttatc cggggttggc
     3361 ggtccaagtc gggtttttcg gggaagcggc gcacgttttc aaaaaaggca tcgccttccg
     3421 ccgtttcgcc ggccggccgg cttttgtccg tccaaatgat tttttcgacg ggcgtttgcg
     3481 ccttcaggcc cgccaattct tttgacaggc cggccgaggc gaacaggaag cgcgccttgc
     3541 agtcattcag gatatacgcg tattcgctgt ttttcaaaaa tgtgttcatc ggtacggcga
     3601 ccgcgccgat ggcggagacg gcgaaatagg cggtaataaa ttctgtggaa ttggaaaccg
     3661 ccagcgcgac cgtgtcgccg aacttcacgc cgatattttg cagatacgcc gcgacggctt
     3721 cggcttcctg cttgagcgcg cggtaggcgg ttttttcctt gccgtcgaac actgccgtgc
     3781 cgttgccgtt tttgcggcag gcggcggcga gcatttcgta gaaattggca taagtccggt
     3841 tcatgaaact tcctttatgg ttgcggacgg cgaacgccct catttgaaaa caatatgccg
     3901 tctgaaaacg gaaggcggca gtttatcatc tttgccttca gacggcatat caaacgccgc
     3961 ctttgcgccg catgccggag gttcgccccc gtccttccta tgtctgacct gaatgccgtc
     4021 tgaatcaata tgaagatata cgtttcagac ggcatccccc tgttgtggaa ataccgtccg
     4081 cccccccctg tccgattatt gcctgattgc ggcggcgcgg acgagttctt cgaggctgtc
     4141 ggcaagcatt tccgccgcgc gccggtagcc ttgggcggag aagtgtacgc cgtctttggc
     4201 ggcccatcct tggttgagcc agtttttcat gctgcatatg ccgcccattg cgttttgcca
     4261 agaccaaaac atcgtctgcc cctgacgggc gacgcgccgc tgcatctgtt ggacttcggt
     4321 caggaggacg gggcgcgtgc cgcatacgcc gagcgtgttt ttcagggatt cgggcgcgcc
     4381 gatgatgagg atgccggcgg cgggcaggct gtcgcggatt tggcggacgg tatccagcca
     4441 tttttgttcg gtatcggcaa tgtcgatgtt gttgttgaag gcttcgttgg tgccgtagga
     4501 aaggataacc aaatcggcgc cggtttgggc aaggtcgttc atacggtcgg cacgccattt
     4561 cgaccactgg gtcaattgtg cgccgttgat gcccatcgcg gaaacggtaa tgccgccggc
     4621 gggattttcg atgttgatga agccgatgtc ccacggcatt tcggtctgta tggccagggg
     4681 cagtgccgcg cccgtatcca gtacctgcca gccgccgccg ttggcggaga cggtgttgcc
     4741 gttgacggtc agggtttgtt cggcgagcag cggtttggca aacagggaaa cgcgctgttt
     4801 gccggttttg ccgtcagacg cggtcagggt catgccgccg ccgctgccgg tttgggcgag
     4861 gatgccgccg agcgggaaat ctccggtatt gttcctgctg gtgaagcttt gccagttgcc
     4921 gctgtgacgg acggccgcca tgcgctgccc tttgacgttg gcggggtaaa cccagcctat
     4981 gccgccgtcg ccccatgttt tttgcaggcg tttgcgcagg gcgtcggtaa agaagtcgcc
     5041 ggcggtatgc gagtcgccga tttgcaggat gcggaaggtc tcgccgctgc cttgtgcgac
     5101 ggatcggagt tttttcatcc aaggcgaggc ggcggcgttg ccgtagtcgg tcagcagccc
     5161 gtttgtatcg gtgtaggggg cggacgggga aacggtaacg gtgtcggggc tgacggaggc
     5221 gacgggcagg gcttctgcct gcgtggcggc gaacagggcg gaaaatgcga tgaagtgttt
     5281 ggggttcatg gctgtgtact tgatggttgc gtactcggtt caaaaacgat tttttccatt
     5341 attttttccg ccagcagttt ttgtccttcg gcggtaaagt gtatgccgtc cttactgcgg
     5401 tagcggacgg gtttgccgtt gacgttgacg gaatcggtgt agcggccttt cccgccgctc
     5461 agtgtttgcg cggtgggaat caggatgatt ttgcctttca agtgttccga aagcagtttg
     5521 tcgaggtagc gcatctgacc gtcgagcttg acttttttca tgtaggggat gccgagccag
     5581 acgacttgga cgcggtgcgt gtgtgcggct tcaaggatgc ggtcgacgcg tttcaggtat
     5641 tcttgcgccc attcgtcgga agcgaatttg aggtagcgtt tgccgacggg gaaatcccac
     5701 gggtcgttcg ggccgaggaa gacggcgagt acgctgattt cgggatgttt tttcaaggtt
     5761 tcttcaatcg ttttcggcca gtcgaagaat gagggatagg aaagccccgt gctttgtttg
     5821 ctgaggttgg cggattcgat gccgtattgc tgtttcaggc ttttttgcac gaaaggcgca
     5881 acgccctgca tcagcgaatc tccggcgaaa aagactttgt cgccgctgcg gacggcggcg
     5941 gcttcggtgc cttgtttcca ttctgttccg ccggccggcg gggcggcttc ggatgcggcg
     6001 gcttcaggcg gcatatctgc cgaaccgccg tcttgagccg tcggcggcgt ttcgccggac
     6061 aggaaggttt tgatgccgtc tgaaagggcg taggcgtttt cttgcaacgc cgctccgctc
     6121 cgccaccatc cgtaggcggc aagcggttcg agcgggctgt tgcggtggta ggtctgctgc
     6181 cagtaggcgt tgatggggtt ttggctgaac cacacggcaa tcagggcaga catcagtatg
     6241 gaggcgaaaa gggaaagaaa gtttttcatg tccaaaccct taaaaattgg cataaataaa
     6301 gccgggtatc cccgaagggg cgaggacgat aatcagcagc aggatgatgg aaatctggat
     6361 aaaccacagc cacatcggga ttttttccaa acctttgacc gtgccgtcga aagcgcgttg
     6421 caggtaaggg tagagcagca tcaaggatgc aaacgaggca agcagcagca tatccgcccg
     6481 ttgcggcgcg ttccagccgc cggcattggc aaagagggca ctgaaaactg cgcctgcatc
     6541 gtcggggttt gcggtattga agacgacaaa gctaaggcag acgaaatgga aggtaacaaa
     6601 ccacgagagc ggcgcaaggt atttcagacg gcatagcgcg tcgcgtccga aataccggtc
     6661 gcccgtgttg agcagcgcca gtgccgtgcc gtgcagcgcg ccccaaatga ggaagttcca
     6721 gccgtagccg tgccagatgc ctgagagtac cattgccgcc atcaggttga gctgtgtccg
     6781 caaaaagcct tttttgctgc cgcccaaggg gatgtagatg tagtcgcgta tccaggtgga
     6841 aaggctgatg tgccatttgt cccaaaatgc gcggatattc gcggcacgaa gcggtgcgga
     6901 gaagtttttg ggcagccgga agcccagcag catcgccata ccgataacca aatcggaata
     6961 tccggaaaag tctaaaaaga gttggaaggt atagccgtac acgcctgcca acacgcccca
     7021 gccgtcgaat tgggcgggat tttcaaatac gggcgacacc cagttttccg ccagcatccc
     7081 cgccagccac cattttttgg caatgcccag taaaatcagg gaaacggcga gggcggggcg
     7141 gacgggcgaa cgcggtcggc gggtgcggat ttgcgccagt gcacccgcct gttcgccgtc
     7201 cgtgcttttg aatgcggcgg cgcggataat cgggccggag gtaacggtgg ggaaaaaact
     7261 caggtgcagc agcagctcgt gccacccaaa acgcgcggcg tgcggggcgc ggaagcagta
     7321 aaccagatag gcgacagact ggaaggtata ataggaaagc cccagcggca tcaggatgtc
     7381 gattgcgccg ccttttccgg catattgtgc aatcagcgga cggaaaaaat cgaaatattt
     7441 gaaaaagccc aaaacggtaa tcgaggcggc aatgccgcac cccagccaaa agcggcgcgt
     7501 actttcgcga tcggaacgga gcagttcgcc caaaaggtac acgcaggagg aataaaggac
     7561 gatgattgcc gcaaatacgg ggctaatatg gtagagccag cccataccgg cagccaaaag
     7621 cagcaggttt tggacggacg ggtatttcgc aaagccccag taaatcggca ggaagacgat
     7681 aaaaaacagt gcgaactcga cagacagcag cggcataaac catccttggg acaaaaaaat
     7741 aggcgttaaa acacggcaaa gcccgttatt ttaagcaggg gcggatattc cttcaatcaa
     7801 aatgccgtct gaagggcggg aggggcttca gacggcatcg ctcggtcagg ttcggatatg
     7861 gtggttcaaa ttcaggcaga cccgcaaacc tgccgccgcc gtcagcatac ccgcaacgcc
     7921 gatgcaggaa atgcccgagt attgcgtaac ctaatgtccc agcagtgcgc cgccgcctat
     7981 accgacgttg taaagctccg aataaatcga gttggcaagg tctgcggcat cggaggcgaa
     8041 atccaaaact ttggcaacca tccccaagct gacgaccaca atcgccgtcc cccatacgaa
     8101 caccaacgcg taaatggcgg caggaaaatg cgccaaaggc agcaaaagcc ccgaagagag
     8161 ggcgataagc gacaccgcgc ccgccaaaaa tgcgcgcgga tgtttggcaa accatttgcc
     8221 gaacaggtaa gacgcggcaa aacccgccag gccgtacagc cccggaacca ccgtaacctg
     8281 ccgcgcggag aaaccgccga tttggatgac gaagggctcg atgtagctgt atgcggtaaa
     8341 gtgcgccgta atcaccggca ccgttaccgc atacagcagc atcagttttt tccgtttcaa
     8401 aagcaggggc aggctgctta aagaaccggt attcacgctc ggcaggcgcg gcaggctttt
     8461 ggcaagcact gccatcaccg ccgccgaaca caggccgatc agcagaaaac tcgcctgcca
     8521 gcccaaatat tgtccgacca tacgcccgag cggaatcccc gccaccatcg ccatcaccgt
     8581 ccccgtgctg agcagcccca atgcctgatt cccttttccg acaagggcga tgcgtaccgc
     8641 cagcgaagcc gtaatggacc agaacaccgc gtgcgtcagc gcaatcccca cgcggcttgc
     8701 gagtaggatt tcaaaacgcc acgaggcaaa cgacaggatg tggctgacag taatacagcg
     8761 caaacaggac cagcagcagg ccgcgccgtt ccataattgc gcgtcagcag catcagcggc
     8821 agcggcagcg aagtcagcgc gacaatccac gcataaaccg taatcatcac acccgtttcg
     8881 gtcgccgcca tgccgaagct tcgtccgatg tcgctcaaaa gcgcgatggg gatgtattcg
     8941 accgtgttga aaataaatgc gccgattgcc aaggcaatga tgctcaacca aggtttgtgc
     9001 gaagcgttca tctcaattcc ttatatgtcg caccccgcca aaagggggcg cattatggtg
     9061 aattaacaaa aaccggtacg gcgttgcccc gccccggctc aaagggaacg attccctaag
     9121 gtgatgaagc accaggcgaa ccggttccgt accatttgta ctgcctgcgg cccgccgcct
     9181 tgtcctgatt tttgttaatt cactatactt ttcttgtctg ccggacggca aaatccaaat
     9241 cagggggaag cagccgcctc ccgttcggag gaacactcgg gacgctgctt tgaaaatcgt
     9301 tccatatgtt gaaacattgc cgcctcccga ttgggcaagg ttgccattat gtcgcaagca
     9361 tccgtgccgc accgtttccg cgtttgccca agttcacgct ccgacttaca ataacgcccg
     9421 tcagattgca gtaccaacga aaggaaaaat cgtggaaacc ctcctcctcg acatcgtcgg
     9481 catgagctgc ggcggctgcg tcaaaagcgt tacccggata ttagaaagcg taaaaggcgt
     9541 ggcaagcgtc gaagtcagcc ttgaaaacaa aagtgcgacc gtcggatacg accccgcgca
     9601 aaccgcagcg gaagcactaa tcgaagccgt agaagacggc ggctatgatg cggcgttgaa
     9661 ataaagcggc aaaaatgccg tctgaagcct ttgcgtttcc ccgatgccgt ctgaagcacg
     9721 ctccgcgcct tcagatggca tttgatttag ttgaacgggc aggatgaatt ggttaaactt
     9781 gcgggcaata gtgccttcag acaaacaaaa tatgccgaac aaaaagaaaa aagccgcctt
     9841 tgcgcgctgc aacggagaac ggtgtgccgg gcgtgtccga catcaaaaat tcatatgatg
     9901 atttgatgta cgaatccaaa gcattttccc aaaccgccat caacgccttg gaggcatgcg
     9961 cccgcatgat gggtttgaag cctacggcag ccgcaaaagc caaggtcttg gagctgggct
    10021 gttcgatggg cagaaacatc atcacgcagg cactttatta cccggatgcg gaatttgtcg
    10081 gtatcgactt gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct
    10141 tggaaaatgt gcgtctggaa gaaaaagata ttttgaccgt cgatgaatca ttcgggaagt
    10201 tcgactatat catcgtccac ggcatttggt cgcgggtgcc cgacgcagtt aaagacaaaa
    10261 tcttttcgat ttgccggaac aacctgaccg aatacggcat tgcctatatt tcatacaatg
    10321 tttaccccgg ttggaagcgg caggagcagt tgcgcgatat tatgcagttt gccggcaggg
    10381 atgcgcttgg agaacccttg gaagcaagga cgcggaaagg cttggatgcg atcaaggcgc
    10441 tggcggaaat tttggaaaac gacaagggct tgggcggcgg caaacttccg gcgattcaaa
    10501 aaatattgaa tcacaatact tattatgtcg cacacgaata tatggaaata ttcaacgacc
    10561 cgatttacgt taacggtttc attgaatggg caaaccgcca ccggctggcg tatatcgggg
    10621 ataccgattt gcatgtgtct tttgtttctt ggatggcgga gcatacgcgg gagcggattc
    10681 tggcattggc agggggtgat tatattgcca aagaatttta cagcgatatt ttatccgacc
    10741 gccaattcca ccgttcgctt ttatgccgtg aggaagtcgg ggatactgtc aggcgtgatg
    10801 agtcggttgc cgtcgaagtg atagaaagtt tgaatttccg cccggcaaga ggggaaacaa
    10861 tcaactttga tgaaaacgat accctgcttt cgggcatacg cgatgtgatg aaaaccggag
    10921 aggcgtttaa aacggaagat gttgcggaaa atcttgcccg ccgatttccc ggtctggaat
    10981 ttgaccgcat gaaaatcaat tcccagcttt tattgcaaac cattctcggg cgtttttctg
    11041 tttcatcaga caatgcgggc aaaccatttt ttgaagacca taaaacctat gtgccggcgc
    11101 gctttacaaa ctatgccgcc gcctttgtgg aacacggtgc gggagcgttt gtccggcctg
    11161 ccaaccgtta caacgaaagc acgccctcat tcggatacgg gcatttgtac attatgcggc
    11221 aattgtcgcg gccgacgagc aaacaggcat tgattgaaac ggttgccgaa aacttgaaca
    11281 tcgtcagcgc cacgcccgac ggtttgacat tccatccgcc tgccgaagtg tatgtggaag
    11341 aaatattggc agacttggca gacaggcatt ttctcgtttc ggcggattga cgggcaagtc
    11401 ccgacggcaa tccgtccgat atattttcag acaggaaaga cacacactgc gaaaaacggt
    11461 acacgacaca tatgaaccag acatttacct tgcccgatac gcgcccgtat ccccaaaatc
    11521 cgattaaaaa ccacctgctg ctcaatgcct accagttggc gcacaattcg tcacaggctt
    11581 cgcgcaaact ctcgtccggg cagcttcaaa ccgaaatcag ggggatgctt gagcaaaacc
    11641 actatatcaa cctttccctc gcgctgacga tgtcgcccga tgccggaact tatgccgcgc
    11701 tgctttccag tgtgaacgcg gtactcgatt gcgagaaaga aggcgaagtg cagtggttcg
    11761 ccctgccggt cgtgctggtg tccggctgca agaaagaacg ggcaatcgag atgaagctgc
    11821 cgacggaggc attgtttgcc tgcctgcaaa actatccgca cctgcgcgcg ttgacgcaag
    11881 agacacaatg gctgccttat cttgtgcatt cttccgattt gagtgcggtc gcgccggatg
    11941 agtggtggcg tgccaaacaa aataccgaag cggcggcgca acacttacgc cgtttcgccc
    12001 cgcgcccttt gctgttgccc gaagggcagt ccgtccacgt cgtttacgtg ctgggtttcg
    12061 gcagcggcaa agttcaggcg gcattggggc agaaccttct tcaggcaggc ttgcccttaa
    12121 tgcaggtatg gcaggaaaat cttgcatcgg aaggcattac gctgtttgcc aatccgcttt
    12181 cccccgatac cccggcgcgc gcgctttcag acggcagcca cacgcgccaa cgtatggcga
    12241 tggatgtttt tgcggcaaac gcgatacgcg ccgtccggat gcagggtccg cgcgtcggcg
    12301 tggtcgctgc ggcaaaggcg ggcggacaga ttctattcgg atttaatgcg accgacggcg
    12361 cgtttgaagt cgtgccgcag gtgttttgct ggcagctttc ctttaccgat aacattgccg
    12421 tcatccagca gaatttcctc gacctgatgg cagaatgccg tgtagaacac gtttacctgc
    12481 tgcacaatcc cttgggcgaa caggaaaaca tcccgagcta tgcggaagcg ttgaaacggg
    12541 aagggcgcaa tccgtttttc agcgcatagt gatttgtagt agtgataggc tttctcattt
    12601 ctataataaa tcaaaataaa gagcacaaca ctttttcatt ctgtgttgtg ccttgagtga
    12661 aacgaaagga tgaattataa atatgaagat tgtaattgca ccggattcgt ttaaggaaag
    12721 cttgacagct caacaggtag ctgaagcaat aaaaagaggc ttccaacaat cgatagcaga
    12781 tgtggaatgt ctcctctgtc ctgtcggtga tgggggagaa ggcactgtag atgctatccg
    12841 acattctctt gacctagaag aaaaatgtct ccaagtgaca ggaccttttg gacaaaaaaa
    12901 gtcatgcgct attttcaaaa agaacaacta gccctattcg aagttgctga tttggttggt
    12961 cttggaaaaa tcccgctaga gaaacgaaat ccattacaaa tccaaactcg tggtattgga
    13021 gaattgattc gccacctcat tagtcaagaa attaaagaaa tctatattgg cgttggcggt
    13081 acggccggta atgatggagg tattgggatt gctgctggtt taggttatca attttatgat
    13141 gaggatggaa atgccttacc cgcttgcggt caatccttac taaacccagc ttctgtttca
    13201 atagaaaatc gctatgaaat tcctgaagat gttcacattc gtattttagc agatgttgtg
    13261 agtcccttat gtggacacca aggtgcgacc tatacgtttg gcaagcaaaa agggctggat
    13321 tctactatgt ttgaggccgt agatcaggca atacaagatt tttatgaaaa agtcccccct
    13381 gcaacattaa aacttaaagg agcaggagct ggtggaggca tcgctggtgg tttgtgtgcc
    13441 tttgctcagg caagtatcgt atctggaatt gacacctgcc tggacttgat tgactttgat
    13501 aagaaagtgt cagatgttga cttggttatc gttggtgaag gaagactaga tcgtcaaagt
    13561 ttagcaggga aagcgcctat tggtgtagca aaaagaaccc ctgtcggagt tcctgttgtc
    13621 gctatttgcg gcagtttcgc tgaagatttg ccttccctac catttgaaaa tatccaagct
    13681 gccttttcta ttttggagaa aagtgaacct ttagaagata gtttgaaaaa tgccagtctt
    13741 tatttggagc atacggcttc taatattgga catttattaa atatgcctaa gatttagcca
    13801 agccatttct tccagatgga tgttttggca ggttcttcct tatgcggtta cgattaaccg
    13861 gccgatgccg tctgaaaggg tttccgcttc agacggcatc gcgttggaac agggatggga
    13921 tatgaaaaaa ccgaaaatcc tttttgtctg cctcggcaac atctgccgtt cgccgatggc
    13981 ggaatacatt ttgcgccgcc gtgccgccga agcgggcatt cccctcgaaa cggacagcgc
    14041 agggacatcg ggctggcacg acggcgagga tatgcaccgt gaaacggcaa agatattgaa
    14101 aaaatacggc attgacgctt caggctttac cagccgcaaa atccgccaaa gcgatgcgac
    14161 ggcgtttgac tacatcatcg ctatggacgg caagaatttg tccgaattgg aaaaaacttt
    14221 tggcaggcgg ccggaaaaaa tattcaagct gaccgacctg atacccgaaa gcggttacga
    14281 ccatgtcccc gatccgtggt acatgggtga ttttgaagaa acatacaggc ttgcggatgc
    14341 aggctgtcgg gcattattgg aaaagatttc caaataaagc aattgaatac aaacacaaat
    14401 ataaaaccgc ctcctgtcgt atcggtcgtt cggacggcat aaacagggaa tttatgaaaa
    14461 caaatttcaa acagaaaatt atcgaacagg cacgcagaga ggacttgcag gtaaccgctt
    14521 tgcgcgagca ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaatg
    14581 ttttgtcaca gatgcagcag cagagcgaag gcgtgcttgc accgcctacc gcctaccgtg
    14641 cccttgattt ttgggcggat cagggcgttt tacacaaagt ggcggcggtc aacggctata
    14701 ttttgtgcag ccacgcgcag cacgagtgca acgaccattg ccacgaccac gaagaagccg
    14761 aagcgcacca cagcgcgttt attttggtct gcaccgaatg cggcgcggcg gacgagcaaa
    14821 ccctcagcca cgagtgggcg gcactgcgtg caggcgttgc cgaaagcggt tttgcgctga
    14881 aagaagaaca cgttgtttta actggaatct gtaaaaaatg tcagaagtga aaaaaaccaa
    14941 agtccacctg atttcaggtt ttctgggaac aggcaaaacc accgcgctca aaagcctgat
    15001 ggcgcaaaaa gacccgaacg aaaaatgggt catcatcgtc aacgagttcg gcgaaatcgg
    15061 cattgacggc gcggtattga gcgacaacgg catccctgtg gcagaaatcg ccggcggctg
    15121 tttatgctgc accgccggcc cgcaaatggg cgtaaccgtg cagaaaatgc tgcgcgacgc
    15181 caaacccgac cgcctgatga ttgaggcaag cggactggcg cacgccgcca gcgtcatcga
    15241 cgaattgaaa gccaaacctc tggacagtct tttggaaatc ggcgccgtct ttaccgtcgt
    15301 cgatccgcgc cagttcatca atcccgatta cgcgcaacag gcgttgtata aagaccaaat
    15361 cggcatttgc gacgtattgg ccgccggcaa aaccgattta tgcacccccg aacagcttgc
    15421 cgaatttcat gacaaagcgg caaaactgtt cccacccaaa gccaaagtgg tcgaagtcca
    15481 aaacgcacaa ctcgatatcc aatggctcga tattcccgta gttgaaaaat cacgctaccg
    15541 cctcaaagcc ctgccggaca acaccatggg cttccagtcg caaggtttca cattccccgc
    15601 cggacgcgat ttcgacggcg aaaaattgac caacttcttc aatgatttgc ccaaaatgac
    15661 cgaaggactc gtccgcgcca aaggcgtgtt ccaagtattg ggaacgtggg tgtggctcaa
    15721 ctgggtggac gggcagtggg gcgcgaacca agtgtcttgg cggcgcgatt cgcgtttcga
    15781 gctgattgcc aaatcgtttg acgcggattt aatcgaacaa aaacttaaag acgcattgga
    15841 atagcgttca cgcgctttca gacggcatat gaaatgaaaa tgccgtctga aggctttagg
    15901 aggacgggat gaaacccaaa ttcaaaaccg ttttaaccgc gctgcttttg gcggtttgcc
    15961 tgccgtctat ggcggcaacc cgcgtcctga tggaaaccga tatgggcaat atccgtttgg
    16021 ttttggacga atccaaagcc tccaaaaccg ttgccaattt cgtgcgctat gcccgaaaag
    16081 gcttttacga caacacgatt ttccaccgcg tcatcggcgg cttcgtcatc caaggcggcg
    16141 gattgaccga ggacttggtg caaaaggcaa ccgataaggc cgttgccaac gaatccggca
    16201 acggcttgaa aaacaccgtc ggcactatcg caatggcgcg gacggcagac cccgattccg
    16261 ccgccgccca attctttatc aatctggcgg acaacggctc gctcgactac aaaaacggac
    16321 aatacggcta caccgttttc ggcagggtcg aaagcggaat ggacaccgtt tccaaaatcg
    16381 cccgcgtcaa aaccgccacg ctcggccttt atcaaaacgt acccgtacag cccgtcaaaa
    16441 tccgtcgcgt tgttgtcggg cagtaacacg cagacagacg ttcagacggc gtcgcccgtt
    16501 tcccaaaaaa cgccgtttaa agtaaaaaaa tatttta
//
LOCUS       DAWTCI010000045        16299 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWTCI010000045 DAWTCI010000000
VERSION     DAWTCI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16299
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.045"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..242)
                     /locus_tag="WM481_001388"
     CDS             complement(<1..242)
                     /locus_tag="WM481_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080690.1"
                     /translation="MPSERLSDGIFPCILNRVYETPDAFGGNVGCCAMFGGDQRTKCA
                     GKDLMPSEGSDGIGIGESEAVAHAQRGFVGFEAGVF"
     gene            complement(260..817)
                     /locus_tag="WM481_001389"
     CDS             complement(260..817)
                     /locus_tag="WM481_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFA9080691.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQILQAATQVSDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFVPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCEDKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(892..2526)
                     /locus_tag="WM481_001390"
     CDS             complement(892..2526)
                     /locus_tag="WM481_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HFA9080692.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELRPFNDT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEALNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEIDKCARATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESAVYYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2924..4030)
                     /gene="serC"
                     /locus_tag="WM481_001391"
     CDS             complement(2924..4030)
                     /gene="serC"
                     /locus_tag="WM481_001391"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFA9080693.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLRTAQQEMSDYNGTGFSVMEMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSDGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINDIHPDARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4396..4827
                     /gene="rimP"
                     /locus_tag="WM481_001392"
     CDS             4396..4827
                     /gene="rimP"
                     /locus_tag="WM481_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFA9080694.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELAAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIGYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIGGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4855..6357
                     /gene="nusA"
                     /locus_tag="WM481_001393"
     CDS             4855..6357
                     /gene="nusA"
                     /locus_tag="WM481_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HFA9080695.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPDTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIAGGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDRLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMNHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LLSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6369..9200
                     /gene="infB"
                     /locus_tag="WM481_001394"
     CDS             6369..9200
                     /gene="infB"
                     /locus_tag="WM481_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFA9080696.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVQPEQTAEDAVKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEPKEKAVKPKHERNGKGKDAKKPAKPAAPAVPQPVVSAEEQAQ
                     RDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQAKAAQEAKTGRQRPAKPAE
                     KPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGKGAKGGRDRNNARNGGDERV
                     RGGKKGKKLKLEPNQHAFQAPTEPIVHEVLVPETITVADLAHKMAVKGVEVVKALMKM
                     GMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLGEGAEAVEAEALPRPPVVTV
                     MGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKTPRGVITFLDTPGHEAFTAM
                     RARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPIVVAVNKIDKDTANPERIRQ
                     ELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLEAEVLELTAPVDAPAKGIIV
                     EARLDKGRGAVATLLVQNGTLKKGDMLLAGTAFGKIRAMVDENGKSITEAGPSIPVEI
                     LGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLAKQQAAKLENMFNNMGETQA
                     QSLSVIIKADVQGSYEALAGSLKKLSADEVKVNVLHSGVGGITESDVNLAIASGAFII
                     GFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMSGMLSPEKKEQVTGTVEIRQ
                     VISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGELASLKRYKDDVKEVRMGFE
                     CGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9360..10700)
                     /locus_tag="WM481_001395"
     CDS             complement(9360..10700)
                     /locus_tag="WM481_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFA9080697.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GGARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAAKLPPELNKARSHWKWSSLNTHRPETSRAERREYAEKIGIRLAGK"
     gene            complement(10774..12114)
                     /locus_tag="WM481_001396"
     CDS             complement(10774..12114)
                     /locus_tag="WM481_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFA9080698.1"
                     /translation="MLNPSRKLVELVRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRDRMLRDTLERVRAGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVNQAVLRLYADQWRQPSV
                     RWKIGATAHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPS
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKTYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQ
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQ"
     gene            12257..12883
                     /locus_tag="WM481_001397"
     CDS             12257..12883
                     /locus_tag="WM481_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFA9080699.1"
                     /translation="MYAGERFNTYSHLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12948..14366)
                     /locus_tag="WM481_001398"
     CDS             complement(12948..14366)
                     /locus_tag="WM481_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9080700.1"
                     /translation="MENILSVLVGTVNRFLWGYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWLSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIVALGFIGNATQANSIASAVTIAFDVPSLAVGIVFAVLAGMVVIGGVNRIANIA
                     RFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMTAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFAFTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWNLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKQ"
     gene            14693..15421
                     /locus_tag="WM481_001399"
     CDS             14693..15421
                     /locus_tag="WM481_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFA9080701.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIGLRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDELMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15480..15968)
                     /locus_tag="WM481_001400"
     CDS             complement(15480..15968)
                     /locus_tag="WM481_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFA9080702.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAVVLACKPKGRVGGLSGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
BASE COUNT         4269 a         4661 c         4158 g         3211 t
ORIGIN      
        1 tggaaaacac ccgcttcaaa acccacgaag cctcgttggg cgtgcgctac cgcttctgat
       61 tcgccgatac cgatgccgtc tgaaccttca gacggcatga ggtctttgcc tgcgcacttg
      121 gtgcgctggt cgcctccgaa catggcgcaa cacccgacat ttccgccgaa cgcatcgggc
      181 gtttcataaa cccggtttaa aatgcatgga aaaatgccgt ctgaaagcct ttcagacggc
      241 attttgtttg agattccgtt taccaatggc tgacaaacgc ttccaaatcg gtattcttgg
      301 gcttatgcac ttcctctgtc ggcgtgccga ccatcatcag cccgatgatt ttatccttat
      361 cctcgcaacc gaaagcctcc cgcaacaatg ggctgttgac ccacatcccc gtaatccaga
      421 cattgtcgaa tccctgagcc gttgccgcca gttgcagcgc atacgccgca caacccgccg
      481 tcagcatctg ctcccattcc ggtttcggct taggcacatc gcggttcggc acaaacgtta
      541 ccccgatgac catcggcgcc atattgccca ctttttccgc ctttttcatc gcatcgtcgc
      601 cgaaattcag ttcggcaacc gtttgcttca acacatcgcg aaaacgttgc aatcctacct
      661 cgccttgaat cacggtaaaa cggaaggggc gcatgttgcc gtgatcggaa acttgggttg
      721 ccgcctgcaa tatttgttcc aactccgccg catcgggggc ggggtgcttc agctttttgg
      781 aagatcggcg gttcgtcaat aattttaaag catccatatc gttatactcc ggtcatcggt
      841 cgggtgtttc ggacacatat gccgtgaagg cttcagacgg catatccggc atcagcgcgg
      901 acggcggcag gctgccaata tatccatttc cttccgatag gtttggctgt tggaaatgtc
      961 catcagcccc aataccgtgc tgaaatagtg gtcgtgcgaa tattcgtttt ccgccgcttt
     1021 ttgtttgagg cattggaaat ctatgccccc gtgttggcgg aaggctttgg aaaaccacat
     1081 aaccatcggg atatgcgtct gtccggaagg cgcgatggcg taaggcgcgg cgtgcaggta
     1141 catcccgttt tcgcccaaac tttcgccgtg gtcggaaaca taatacaccg cgctttccaa
     1201 atcgtcgcgg ttttcaagtt tgcggataac cttgtcgata aactgatcca catacaaaac
     1261 cgtattgtcg taagtgttga ccaacgtggc gcgggcgcat ttgtcgattt cgttggtatc
     1321 gcatgtcggc gtgaatttgc gctcggcttc ggtgtagcgt tcgtaatacg tcggcccgtg
     1381 gctgccgatg gtatgcagga ttaaaaccgc gtctttatcg tttttgttga gggcttcgtc
     1441 gaacttggtc aacaggatat tgtcgaggca ctcgccgttg cggcagtatt cgggcaggtt
     1501 gagcgaagta acgtcggtat tcggcacttt gccgcacacg cccttgcagc cggaatcgtt
     1561 ttccaaccaa gtaacttcca cgccggcgcg ctgcacgatg tccagcaggt tgtcttggtg
     1621 ttcggctttg atttcgtcat aatccgtgcg gtcgaaggtt gagaacatac acggcaggga
     1681 gtgcgcggtc gatgtgccgc agcttctgac ctgcgggaaa ttgacaattt catcgccgcg
     1741 cgcggcaagc agcggcgtag tttggcggct gtaaccgttc aaaccccagt tggcggcacg
     1801 cgtagtctcg cccacgacca gcaccacgaa acggcgcagg ctgccggccg gccggttttg
     1861 cacgaccgcc atatccaatt gcgtataagg aatattggaa cgcttccaat ctttgtattt
     1921 cgacacgccc gcgccgatga aattagacgg cacaatcaga tgggttactg atttattgtt
     1981 gcggaaaaac gaggcgtaat cctgatattg caacatcgcg atgcccaacg cgcacaaaaa
     2041 ggaaacggcg gcaagcacaa ggcgcgtcaa aagctcctta taccaaacgc ggtatttgac
     2101 cttgacggcg atatatgcca gcgcgggcaa tacgcccaaa catacaatcc acagcacata
     2161 gcccggcgta atcaggcgcg cgctttccgc cgccgtggtt tgcaagacat tattcaacat
     2221 cgacttgttg aaatagatat tgaaaaatat ttcttggtaa gacaccgccg cactgataac
     2281 caatatcaat ggaatcaaca ctttatgcac aaaaggcagg gcaatgacgt gaaaaacgaa
     2341 attacttaaa aaaaacagca ccaccggcat cgtatagagg aagatgtccg ccccggtgtc
     2401 gttaaaaggg cgaagttcga caactttggc aaaaaaggcg taattcaata ccagcgaaga
     2461 atacagggaa agaaaggcaa tcagcgcgga agagccgagc ttcggcctca ggttcggttt
     2521 tatcatttgg aatgtgtcgg ataagggttg gaaaaggcat ccggaatccg gattattgaa
     2581 aaagattctt aattataagg caacggagca aagcagggca agaaaacgag taggcggcgc
     2641 gggggtttcc gcccgccatt caaacgtccg gcagacataa aaacatcgta agcaaaattc
     2701 gaaccggtct gcaaccgccc ctgccggaaa agcgggcaaa gcatttcata ttggaaaaac
     2761 ccggccgcgc cgccgacggg acagtccggc acaaacagca tcacgcccgg attgaaaagg
     2821 acggatagcc gccggcagca caatcttacc acctccaaaa cgccgccgga gaacgccgaa
     2881 acagccgatg ccgtctgaaa cgcattcggg cggcatttga aaatcaaccg taacgccgtt
     2941 ggaaatcgcg cataaaatct gccaaagccc gcacgccttc aagcggcatc gcattataaa
     3001 tgctggcgcg catcccgccg acagacttat agcccttcaa caggcacaag ccctgcaatt
     3061 cggcttccag cacaaaacgg cggtccaagt cttcgcttgc ggttttgaag accacgttca
     3121 ttttggagcg cgcatcagga tgaatatcgt tgatgtaaaa accgccgctg ccatctatcg
     3181 tctcatacaa ggtttgcgcc ttcagccgat tgaccgcttc aatttttttc acaccgccct
     3241 gcgcctgcag ccagcggaac accagccccg acatataaat cgcgtaagtt gacggcgtgt
     3301 tgtacatacc gtcgcggttc aaatgagaac ggtagttgaa cacatcggga atatcgttcg
     3361 gacaacgctc gagcaaatcc tcacgcacaa tcaccaccgt aactcctgcc ggcccgatgt
     3421 ttttctgtgc gcctgcgtaa atcagtccgt agtcggcaac atcaaactca cgcgacagaa
     3481 tctcgctgga catatcgcac accaacggcg gcatgccgtc tgaaaggcgc ggcacttcac
     3541 ggtattgcag cccgttgaca gtttcattga cggcaaaatg gacaaacgcc gaatcgggtg
     3601 caacatccca cgtttccaca ggcggcaggt caagatagtc gaactgctcg cccccgtgcg
     3661 ccgccaaacg gatttccgta tcggtcaaac ggctcatctg ttcataggcg atacggctcc
     3721 agttgcccgt taccaccgcg tcggcagtgc ggaaaccgtg tgccagattc atggctgcca
     3781 tattaaattg ggttgttgct ccgccctgaa gaaacaatat cttatagttg tcaggcactt
     3841 tcaaaagctg cctcaaatcc tgttccgcat gatgcaggat gctcaaaaac atttccgaac
     3901 ggtggctcat ttccatcacg gaaaaacccg taccgttgta gtccgacatt tcctgctgcg
     3961 ccgtcctcaa cacagcttca ggcaacacgg cagggccggc ggaaaaattg taaatcggat
     4021 aaagagacat gatgcagccc tggttctgaa caataacccg cccgatttta ggcttgcagc
     4081 aggctttgtg caaggatggg aaatacctgc cctccgcccg attccatgcc gcccgaacac
     4141 gggaaataat atcaatatat tgatttacaa acataaaaat catgctagcg acaaatagat
     4201 acatttgttt tgtcaacaat attcacgatt tcccattaca aacctcccgt acacccgctt
     4261 ttttccgtcc caaaaacaca aaataaatca acactttcat ttctccgcaa aagcggttat
     4321 aatcacgccg atttttcaac ttttgacgaa aaatggccac ggggccattt ttttattatg
     4381 tacataggga gcagcatgga tattcaaacc atcctcgaaa aaaccctgcc cggcctggga
     4441 tacgaactgg tcgatttcga actggccgcg caaggaacat tgcgcgtgtt catcgacaaa
     4501 gaaggcggca ttaccgtcga agactgcgcc accgtcagca accacttgag ccgcgtcttc
     4561 atggttgaag acatcggcta caaaaacctg gaaatttcca gccccggact cgaccgcccc
     4621 ttgaaaaaag ccgccgactt cgtgcgcttt gccggtcaga atgccaaaat caaaacccgc
     4681 ctgccgatag gcggtcagaa aaactttatc ggtaaaatcg aaggctgcga aaacgacact
     4741 gttaccgttt ccttcgacgg caaaaccgtg caaatcgaat tgggcaacat cgacaaagcc
     4801 cgcctgcgcc ccgaattcaa attctaaaac acagcaatat tggagatgtt caaaatgagt
     4861 cgtgaaatgt tacagctggc agaagcactg gcaagcgaaa aaaacgttga tgcggaagtc
     4921 gtcttccaag cactggaatt cgcactctct accgccgcca agaaaaaggc agaccgcgaa
     4981 cacatggacg tgcgcgtcca aatcaaccgc gataccggcg aataccaaac cttccgccgc
     5041 tggctgattg tggccgatga agactatacc tatcccgatg tcgaaaaaac catcgaggaa
     5101 atccaagagg aaatccccga tacgaccatc caaatcggcg aatactacga agagcagctg
     5161 cccaacgaag gcttcggccg ccaagcggcg caaactgcca aacaaatcat cctgcaacgc
     5221 atccgcgatg ccgagcgcga gcagaatctg aacgagttcc tcgccgtcaa agaagacatc
     5281 gtgtccggca cggtcaaacg cgtcgaacgc cacggcatca tcgtcgaagt cgttgccggc
     5341 aaactggacg cgctgattcc gcgcgaccaa atgattccgc gcgaaaactt ccgcagcggc
     5401 gaccgcatcc gcgccctctt cctgcgcgtc gaagaaatcg gcaacaccgg ccgcaaacaa
     5461 gtcatcctga gccgtacttc cggcgatttc ctcgtcaaac tgtacgccaa tgaagtacct
     5521 gaaattgcag gcggcatgct tgaaatccgc gctgtcgccc gcgacccggg ccaacgtgcc
     5581 aaagtcgccg tcaaagccaa cgaccagcgc atcgatccgc aaggcacctg tatcggcgtt
     5641 cgcggttcgc gtgtcaatgc cgtcagcaat gaattgtccg gcgagcgcat cgacgttgtc
     5701 ctttggtcgc ccgagcctgc ccaattcgtc atgagcgcgc tctcacccgc cgaagtcagc
     5761 cgcatcgtca tcgacgaaga caaacacgcc gtcgatgtca tcgttgccga ggaccggctc
     5821 gcgctcgcca tcggacgcgg cggtcaaaac gtgcgccttg cttccgacct gaccggctgg
     5881 cagctcaaca tcatgacttc cgccgaagca gacgaacgca atgcggcaga agatgccgcc
     5941 atccgccgcc tgtttatgaa tcacttgaat gtagatgaag aaaccgccga cgtactggtt
     6001 caagaaggtt ttgcaacctt ggaagaagtc gcctatgttc ctgccgccga actgcttgcc
     6061 attgaaggat ttgacgaaga aatcgtcgat atgctccgca accgtgcccg cgatgccatc
     6121 ctgaccatgg cgattgctgc cgaagaaaaa ctgggcgaag tgtccgacga tatgcgtaac
     6181 ctcgaaggcg tagatgccga tatgctcctc agccttgccg aagcaggcat taccacccgc
     6241 gacgacttgg cagagcttgc cgtggacgaa ctgattgaaa tcaccggtgt aaacgaagaa
     6301 actgcaaaag ccgtcatcct gaccgcacgc gaacactggt ttaccgaaga caaataaagg
     6361 gggtacagat gagtaacaca accgtagaac aatttgccgc cgagctgaaa cgccccgtcg
     6421 aagacctgtt gaaacagttg aaagaagccg gcgtcagcaa aaacagcggc agcgattccc
     6481 tgacgctgga cgacaaacag cttctgaacg cctacctgac caagaaaaac ggcagcaacg
     6541 gcggcaccat cagcatccgc cgcaccaaaa ccgaagtcag caccgttgac ggcgtaaaag
     6601 tcgaaacacg caaacgcgga cgcactgtca acattccttc tgccgaagaa ttggcagcac
     6661 aagtaaaagc cgcccaaacc caagccgcac ctgttcagcc ggagcagacg gcagaagacg
     6721 cggtaaaagc ccgagccgaa gctgccgcac gcgcagaagc ccgtgccaag gcagaagcgg
     6781 aagcggcaaa actgaaagcg gcaaaagcag gcaacaaagc caaacctgcc gcgcagaaac
     6841 ctaccgaagc aaaagccgaa accgcacccg ttgcggcgga aaccaaaccc gccgagccca
     6901 aagaaaaagc cgtcaagccg aaacacgagc gaaacggcaa aggcaaagat gccaaaaaac
     6961 cggcgaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa gaacaggcgc
     7021 aacgcgacga agaggcgcgc cgcgccgccg cattgcgcgc ccaccaggaa gccctgttga
     7081 aagagaaaca ggaacgccag gcacgtcgcg aagccatgaa acaacaggca gaacaacagg
     7141 caaaagccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct gccgagaaac
     7201 cgcaggcagc cgcaccggcc gtcgaaaaca aacctgtcaa tccggcaaaa gcgaaaaaag
     7261 aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc aaaggtgcaa
     7321 aaggcgggcg tgaccgcaac aatgcccgca acggcggcga cgagcgcgta cgcggcggca
     7381 agaaaggcaa aaaactcaaa ctcgagccga accaacacgc cttccaagca ccgaccgaac
     7441 ccatcgtaca cgaggttttg gttcccgaaa ccattaccgt tgccgatttg gcgcacaaaa
     7501 tggcggtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg atggttacca
     7561 tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc ggacacatcg
     7621 gcaaacccgc cgccgccgac gatcccgaag ccttcttggg cgagggcgcg gaagcggtgg
     7681 aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc gaccacggca
     7741 aaacctcgct gctggactac atccgccgcg ccaaagtggt acagggcgaa gcgggcggca
     7801 ttacgcagca catcggcgcg taccacgtca aaaccccgcg cggcgtgatt accttcttgg
     7861 acaccccggg ccacgaagcc tttaccgcta tgcgcgcgcg cggcgcgaaa gcaaccgata
     7921 tcgtgattct tgtggtcgcc gccgacgacg gcgtgatgcc gcaaaccatc gaggcaattg
     7981 cccacgctaa agcggcaggc gttccgattg tggttgccgt caataaaatc gataaagaca
     8041 ctgccaaccc cgaacgcatc cgtcaggaac tgacccaaca cgaagtcatc cccgacgatt
     8101 ggggcggcac ggttcaattc atcgacgttt ccgcgaaaaa aggaacgaac atcgacgcgc
     8161 tgctcgaagc cgtattgctc gaagccgaag tattggaact gaccgcacct gtcgatgcac
     8221 ccgccaaagg catcatcgtc gaggcgcgtt tggacaaagg gcgcggcgcg gttgccacat
     8281 tgctggttca aaacggtacg ctgaaaaaag gcgacatgct gctggccggt acggcattcg
     8341 gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc ggcccgtcca
     8401 tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac gcgatggtat
     8461 tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa taccgcgacg
     8521 tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat atgggcgaaa
     8581 cccaggccca atctttgtcg gtcatcatca aggcagacgt tcagggctct tacgaggctt
     8641 tggcgggcag cctgaaaaaa ctgtccgccg acgaggtgaa agtgaacgtg ttgcacagcg
     8701 gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcttcgggc gcgttcatta
     8761 tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat gaaaacgtgg
     8821 aaatccgcta ctacaacatc atctacgatg ccatcgacga cgtgaaggcg gcgatgagcg
     8881 gcatgctttc tcccgaaaag aaagaacagg ttaccggtac ggtcgaaatc cgtcaggtca
     8941 tctccgtttc caaggtcggc aacattgcag gctgtatggt caccgacggc gtggtcaaac
     9001 gcgattccca tatccgcctc atccgcaaca acgtggtcat ccacacgggc gaactggctt
     9061 cgttgaaacg ctataaagac gacgtaaaag aagtccgcat gggcttcgag tgcggtctga
     9121 tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc gacatcgtcg
     9181 aagttgcccg caccctgtaa ttcctttgca aataaaatgc cgtctgaagc gttcagacgg
     9241 caacgaaacg ggttctgtat catacagaac ccgttttttg tcgcaaatcg gcttcagcga
     9301 ccctcctgcc ttatcccgat ttgaatctga cttgccatac aaacaggctt cagacggcat
     9361 tatttgcccg ccaaacgtat cccgatcttc tccgcatatt ccctgcgttc ggcgcggctg
     9421 gtttccgggc ggtgcgtatt gagcgacgac catttccaat gactgcgggc tttgttgagt
     9481 tcgggcggga gtttggcggc atcccacggg gctttgcggc tgtgcagctc gatatccgac
     9541 tgtgccgcgt gtccgcgcgt ttgcaggacg tggagcagat cgagggcgcg ggcggcgagc
     9601 agggtcaggg tttcagggtc ggtgtgcagg gtttgacggc cggcgagttt gtcggaaatg
     9661 gtgcgggtat tgggcaggat gccgcccaaa aagccgccga ttgccgtacc caatccgagc
     9721 gagccgccga gtgtggcgat gtccagcccc aagccgatga gcgcgccggt tgccgcgccc
     9781 gtgccggtgc ggatgccgta ttgtttgagc aattcgctgt cgaacgggtc ttggcggaag
     9841 gcttgcggca tccagtcgcc gccgtcgatt tcgctgtggt agaaacggta aagggcaaac
     9901 agccgctgct gcatctgccg ttcgagttgg cgtatttccg cctgcatggt ttgcagcacg
     9961 gtggcggtat cctcgttttc gtccacttcc tgcctgaagg cggcggcatc gagcaggaaa
    10021 tcggcgattt cgcggcgcgc tccgccgtcc agccgctgcc attcgcgccg gcgcatggct
    10081 gtcaggcggt caagtgtgct gcgttcgggc aacatggtgg cgaggttttc ccacaggcgc
    10141 aattcgcctt caaaatcaaa ggcgacggtg tcgaaccctg cgaaaacgtg caggtttctc
    10201 ctcgccagca tggttgtcca cgattcggga agctgtccgc cggtaaagtt gaacacgggc
    10261 ataaccggtt tggcacacca tgaaaggatg gtcaattcat cccgatattt gtcgaggacg
    10321 ggttcgcgcg cgtcgatgac gtacattgcc atatcgcttt gcaaaacctg ccgcagcact
    10381 ttggcttctt ggttgaaatc gtgatgcgcg ccgtggctgc cgagaaactg ttgcagccgc
    10441 tcgatgccgt ctgaacgatt gtccgtatgg ttttccagcc attccaaaac accgcccgcg
    10501 tcttcgagtc cgggcgtgtc atacaggaaa accaacatgt ccgcgccgtc gctgatggcg
    10561 gcttcttcga catggcgtgt ggtggacggt gcgtttctga cttcgccgaa gccgctgtcg
    10621 cgcaacaggg tgcgcaggag cgaggttttg ccggtgttgg tgtgtcccac gacggcgagg
    10681 gaaaggggtt gtttgttcat gatgtttttg aagaatggat tttcagacgg tcttttttca
    10741 gaatggcggc ttaacagaac atttcaagtg agtttattgg tctttcaaac ggccttcctg
    10801 cgccaccctg tcaggctcaa gccacgccgc gccgcattcg gtcagcgcgt tacgccaatg
    10861 ttccagcttt tccgaaaggt cgtctgaaag cccctgttcc gccaaaagct gcaccaccgc
    10921 gccgccctgc gccgcttccg aaagccgcac aatctgccgc agcacgcccc ggtccggcac
    10981 agtttgggcg cgtacgccga taagcagttg cgccggtttc tgcttcagct ctgtctccag
    11041 cgcggcaacc tgttcccgat tggcggcaac gcccttatcc agccattcct gcgccagcct
    11101 gccctcgaac cattggccgt cctgccactc ggtctccagc atgagcgccc atttcggcgc
    11161 atcgttcaag acgattttcg gcgaaacggc ggacacggtt tcccgacgcg tatccgcatc
    11221 ggtgattttg ttctgccagc ggcggatgac cgcctgataa taggtttttt ccaaatccaa
    11281 tccgttttcg cttgttttca aaaggatttt acacactacc caagccaaga ggcgcggcag
    11341 gatgccgtag cagacgatac tgccgaccag cagccccgac caagcccgcg catcggcaat
    11401 attgccgttc agacgacctt cgatgaccgc ccgcgcatcg gggacaggga aaccgagttt
    11461 cgacggcagc catgccaaca tttccaccgc gcgtaccgaa gcggcattgc tcaacagcgt
    11521 gctttcccag ttgaacgtat attgccgcac caaaagcagc agcaataccg acaccagcat
    11581 tccgagcagc gtgcagagcc acaagctgtg cgccgttgcg cctattttcc atcgtaccga
    11641 aggttgccgc cactggtccg catacagccg caacaccgcc tgatttacag ggcctttgcc
    11701 ccgaaaccac gtcgccggac tgctgaaaaa ccgccccact ttcacgcgca ggaacaacgt
    11761 tgccaaccat actgccagca tcagcgtatt catgcccaac actcccgcca aaactaaaaa
    11821 gaaattcagc ccctgattgt ccatcagaag ataagtgcct gaaaatccgg cggtaaacat
    11881 catcgatgcc gccaccaccc ataaccagaa cgaccccgca cgcacacgtt ccaacgtgtc
    11941 ccgcagcata cggtccctgt cgatcatctc cgcccgacgg atgatttttt cctccgtact
    12001 gccgtccacg cggcgcaaag cctccgtcgc ctgcacggga tcgccgctga aaataaaacc
    12061 gccttcatcc aaaatacgga ccagctcaac cagttttcgg gatggattca acataaaatg
    12121 ccgtctgaaa ataaaaaaca aattttaaca cacgcatttt caagaatatt cacagtgtag
    12181 gcaaagagta aatcccacac agaggcaaaa gtatcggcgt aaactgactg cctctacttt
    12241 cccgaaagat tgtgcgatgt atgcaggcga acgcttcaat acttacagcc atttgagcgg
    12301 tttgattctg gcggcggcag gtttgatgct gatgctgctg aaaaccatag gacacgggga
    12361 cggctaccgt atcttcagcg tatcggttta cggcatcagc cttcttctgc tctatttgag
    12421 ttcctcgctg taccacggaa ttgcagccgg aaaactgaaa agcattttga aaaaaaccga
    12481 ccactgcatg atttatgtgc tgattgccgg aagctacaca ccgtttgcac tggtttcttt
    12541 gagaaacggg ccgggctgga cggtattttc actgtcctgg ctgctggcgg ctgcaggaat
    12601 cgcacaagaa ctcaccatcg gacggaaaag cgaaaaacgt ctgctgtcta ttgcgattta
    12661 tatcgtaatg ggctggatgg tcttggcggt aatgaaatcc ctgacagcct cactcccgcc
    12721 ggcaggactg gcttggctgg cggcaggcgg tatgctgtac agcgtcggca tttactggtt
    12781 tgtaaacgat gaaaaaatcc gacacgggca cggaatctgg catctgttcg tattgggcgg
    12841 cagcataacc caatttgtca gcgtgtacgg ttatgtaatc tgaatgccgt ctgaaacaca
    12901 aaaacctccc aaacctgaag gcagggaggt tttctctttg ccgggcatta ttgtttatcg
    12961 tggaactcgt ggaattcata ctgataggac aaatcccgac ccgctttttt ctgtgccaaa
    13021 taatcatcat aaatggcgcg gatttcctta cgcagcaaga acagggcaat caggttgggg
    13081 ataaccataa agccgttgaa catatcggac agattccaaa ccaaatcgac tttgccgagc
    13141 gtacccaaaa cgatagcaag cagaaccaat gcgcgataga tgcccaagtg tcttcccctg
    13201 aaaagaaaac ggatgttgga ctcgccgaaa taataccagc cgataatggt cgtaaaggcg
    13261 aagaaggtca gacacacggc aagcaattgc gaaccgaagc ccggaaatgc cttgttaaag
    13321 gcaaattgag taaccgccgc gccctgttcg cccgaaaggt tggcatcggt cagcaggata
    13381 atcaatgccg tagccgtaca taccaaaatc gtatcgataa atacaccgac aaatgccgtc
    13441 ataccttgct gaacaggatg cttcacatcc gcagtcgcgt gggcgtgcgg agtcgaaccc
    13501 atacctgctt cgttggaaaa cagaccgcgc gccacgccga aacgtatcgc ttcacgcata
    13561 ccgatacccg cagcaccgcc caaaacggcc tcgggattga aggcggcggt aaagatgtgg
    13621 ttgaacatcg gcacaatatg gtcggaaaat tcaaacagga taacgacggc gcacaaaata
    13681 taaacaaccg ccataaacgg cacgacaaac cgggcaatat tggcaatacg gttcacgccg
    13741 ccaatcacaa ccatgcccgc aaggacggca aacacaatac cgactgccaa agaaggcaca
    13801 tcaaatgcaa tggtaacggc agaagcgatg gaatttgcct gagtcgcatt gccgataaag
    13861 cccaacgcga caatcagcgc gatggagaaa aagcccgaca ggaaacgcgc cgcgcccctg
    13921 ccgattttcg gagtcagacc gtgcgtaata taaaacgccg gcccgccgat gtatttgccg
    13981 tggctgacga cgcggtattt ctgcgccagc agtgcttccg caaaaatcgt ggacatcccc
    14041 aaaacggcag aaagccacat ccaaaaaatc gcacccggcc cgcctgcggt aatggcggtc
    14101 gccacaccgg cgacgttgcc cgtaccgatt tgcgcggata tagcaaccgc caacgcctga
    14161 aactgcgata aggacttatc gtctttatca cctttggcaa acaagccgcc gaatacggat
    14221 ttgaatcccg cgcccagctt ggtaatctgc ggcgcaccaa gatacagcgt aaaaaacagg
    14281 ccgataccca aaagcgcata aatcagcagg tagccccaaa gaaaccgatt gactgtaccc
    14341 accagaacag acaatatatt ttccataaaa taaaccttat cttacgatta aaatgactgt
    14401 tttccaaaag acattccaat aaggaaacac ggcgagcaga ccgtatttgc cgcaacagat
    14461 gccttaaatt gtcaataatc cgagagaagc tgtgccagca taccgtaaaa tatcgttaat
    14521 taaaatattt ctttattttt aagcggaaag cggaggaaat cgttttcaga cggcatcgac
    14581 aacggcacgg cataaaacag gatattttgg gtacttgcaa cttatgttaa aatgccgacc
    14641 gtaaaaaatc tgacaaaaac agattaatta tttgaaataa gaaaggaaat ttatggcagg
    14701 ccatagcaag tgggcgaata tccagcataa aaaagcccgt caggatgcca aacgcggcaa
    14761 aatcttcacc cgtttaatca aagaaatcac cgttgcggcg cgtatgggcg gcggcgatcc
    14821 cggcgcaaat ccgcgcctgc gtctggcttt ggaaaaagca gccgaaaaca atatgcccaa
    14881 agacaatgtg caacgcgcca tcgacaaagg tacgggtaac ttggaaggcg tggaatacat
    14941 cgggttgcgc tacgaaggct acggcatcgg cggcgcagct ttgatggtgg actgcctgac
    15001 cgacaacaaa acccgcaccg ttgcggacgt acgccacgca tttaccaaaa acggcggcaa
    15061 cttgggtacc gacggctgcg tggcgttcaa cttcgtgcat cagggctatt tggtattcga
    15121 acccggcgtt gacgaagacg agctgatgga agcggctttg gaagccggtg cggaagacgt
    15181 ggttaccaac gacgacggtt ccatcgaagt cattaccgcg ccaaatgatt gggcgggcgt
    15241 aaaatccgct ttggaggcgg caggttacaa atccgttgac ggcgacgtta cgatgcgcgc
    15301 ccaaaacgaa accgaactct ccggcgacga tgccgtcaaa atgcaaaaac tgattgacgc
    15361 gctggaagac ttggacgacg tgcaagacgt ttacacttcc gccgtattga atctggactg
    15421 atacgcagca cagcagacat acaacaaaat gccgtctgaa cctttcagac ggcattgatt
    15481 tatttagccc ttgcccacgc ccaccaaacc gtcaggacaa ccgccaaaaa atacgccgcg
    15541 ctccaaacag gcaaagcaac ccctaacaga taatccggtt cggcacaatt tccgaacccg
    15601 cgcacaacag gctcgaacca atcaaacaaa ggccagccct tcaatcgaaa cgtccacggc
    15661 gcgccgcacg aaggagccgt acccggcggc agcgactgca accacaactg atatgccgca
    15721 acagaaatac ccgtaacggc gggaatactg ataaacactg cgcctgacag tccgcccacc
    15781 ctgcccttgg gcttacacgc cagcacaact gccgcacaca atgcggttgc caaaacacat
    15841 aaccgctgac tgatgcacaa aacgcaaggc tccataccca aaacatactg tgccgccaaa
    15901 gaaccggcaa atgcacagac cgaaacggca aacagcagcc aaacggcttt tctaaataac
    15961 ggggtcattt tctcaacaca ccaatcaaaa taccgatatg ccgattttgc tggatatatc
    16021 ccgagatggc aagggacgaa tcgacaccgg cacaggtgcg gacgatttcg gttttcccga
    16081 tatgcacaaa acctgacaaa ggacaaaacg gcgatttgtc cgcacaatgc ccgaaaaaat
    16141 acatccggag gatttgaatt tgaatttcgg caaatttagc gaatcaaaag caacagccga
    16201 taaaaaaatc aaaagcttat ttcaatgttt caacacacag gacgacacat aaagcaccgc
    16261 cctatgtgcc gtcctgattt ggaaggggtt acacccctc
//
LOCUS       DAWTCI010000046        16123 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWTCI010000046 DAWTCI010000000
VERSION     DAWTCI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16123
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.046"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            338..2393
                     /locus_tag="WM481_001401"
                     /pseudo
     CDS             338..2393
                     /locus_tag="WM481_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            2518..4068
                     /locus_tag="WM481_001402"
     CDS             2518..4068
                     /locus_tag="WM481_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFA9080703.1"
                     /translation="MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFL
                     TVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFK
                     GMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     DMVSGQPH"
     gene            complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="WM481_001403"
     CDS             complement(4492..6276)
                     /gene="lpdA"
                     /locus_tag="WM481_001403"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFA9080704.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVIKEVKVKVGDKISEGGLIVVVEAEGAAAAPKAEAAAAPAQEAPKAAA
                     PAPQAAQFGSAADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKS
                     RKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGVCTDLPPQKKK"
     gene            complement(6294..6728)
                     /locus_tag="WM481_001404"
     CDS             complement(6294..6728)
                     /locus_tag="WM481_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080705.1"
                     /translation="MSYLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKAT
                     HGPSVKNAVYAHQIETDLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQY
                     SIFEYEVEHPENPDEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="WM481_001405"
     CDS             complement(6829..8391)
                     /gene="aceF"
                     /locus_tag="WM481_001405"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFA9080706.1"
                     /translation="MSIVEIKVPDIGGHESVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAAEAQPAPAAAGG
                     ATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAV
                     FLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAAVPTSASPAAAKIDEAAFAKA
                     HAGPSARKLARELGVDLGQVKGSGLKGRIMGDDIKAFVKSVMQGGAAKPAAAGASLGG
                     GLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEADMTELEEF
                     RKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFNIGFAADT
                     PNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSLGGIGGTG
                     FTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAGMRFTVFL
                     ANLLKDFRRITL"
     gene            complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="WM481_001406"
     CDS             complement(8541..11204)
                     /gene="aceE"
                     /locus_tag="WM481_001406"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFA9080707.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLLKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            11903..13615
                     /locus_tag="WM481_001407"
     CDS             11903..13615
                     /locus_tag="WM481_001407"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFA9080708.1"
                     /translation="MKASQFFISTLKEAPAEAAFASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHER
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQATYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGELVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFTGKVYADFATEKGADWVIGANEDDYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRGKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADRIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAVAIGEIVARVTA
                     SLNA"
     gene            13678..14334
                     /locus_tag="WM481_001408"
     CDS             13678..14334
                     /locus_tag="WM481_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFA9080709.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHVEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLIGRDSDGTGEGEDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAADRIGNQDKVLFSLKQNGVLKQRGETG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVCASFTIGC
                     "
     gene            complement(14344..14799)
                     /gene="ruvX"
                     /locus_tag="WM481_001409"
     CDS             complement(14344..14799)
                     /gene="ruvX"
                     /locus_tag="WM481_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFA9080710.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHADGTEHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVLGKKRKSVLDQVAAQAILHGFLEGGPAECFNGREG"
     gene            complement(14792..15340)
                     /locus_tag="WM481_001410"
     CDS             complement(14792..15340)
                     /locus_tag="WM481_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFA9080711.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDGIALTS
                     SRDVLENISREGAVDKALISIGYSSWSKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(15342..15902)
                     /locus_tag="WM481_001411"
     CDS             complement(15342..15902)
                     /locus_tag="WM481_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFA9080712.1"
                     /translation="MNRLLLLSAAVLPTACGSGETDKIGRASTVFNMLGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKRGTGFAFKSRQIVRYYDPKRKAFAYLVYSDKIVQGSPKNSLSAVSCFGSGIPQTDG
                     VQADTSGKLLAGACIISNPIKNPDKR"
BASE COUNT         3820 a         4009 c         4318 g         3976 t
ORIGIN      
        1 aaaccaatat ttaacagtcg gatagctccg cctgattaag tttttcggta atagttgtga
       61 ttatcaaata taattgccgc ccgaaatatc cgataagttt gtttgtttta tctttaattc
      121 cccaatactt aaaaaagaga gcgttttcat gaacaaaaaa cttgccttaa tgccgctgtt
      181 gattttgagc gcgttttcat ctgccgccga caatactccg cagcatggcg aactcggaca
      241 ggttcatgtc cgcgccgatg ccaagcgcgt caaagccgcg cattcttact ccatcgccag
      301 cgatggcgac ttgcgcgacc gcgtaaattt gggcgtattg ggtaaagcca acgcgtttac
      361 cgcgccgatt accgtcgtca actacgacga acaagccttc aacaataccg aggcgcgtac
      421 tttagtggat gccgtagcca aaaaagacgc ttccgtttga gagtttggcg gcgaaagcaa
      481 cacgctgacc ggtctgtatt tccgtggtta tcagcttgat gcgcgccaat tcagcgtaaa
      541 cggcttggca ggaatgtatg gcacgcaagg cacggcgagc gtgcaagtcg gcttcgcaca
      601 actgattaaa ggcgcgtcca ccgccgtaaa cggcataccc tgaaggcgcg gtatccggtt
      661 ccgtcaacat cgaaaccaaa aaagccgccg acgaaggcaa ccgcaaaatc ggtttggccc
      721 gattcggcaa caaccgcgcc caaggtaggt tcgacttagg gcaacgcttc ggcgaaaaca
      781 aagcattcgg cgtgcgtgcc aacggcaaac tgcgccacgg cgacaccccg cgccacggtt
      841 acagagaaga caacaaagaa tttgccttaa atgccgatta tcgcggcgaa aaactgcgcg
      901 tgacgttcga ctccatctac gcgaaacgca aaatcaacgg cggccgcgcg cgtatgcagg
      961 atatccaaaa cgccggcgga cgcttgttcg acgcgcccga cggcaaaatc aacctgctgc
     1021 cttcttggaa ctggcaaaat accgtcggcg aaaccaatat gctgacgttt gaatgggatg
     1081 cgtttgacaa tacctaaatt accggcggta tcggctacaa caaagcgcgt tattacggta
     1141 cgctgatttc cccaaccgtt tgcggtacaa gcggtgcgag cagccaaacc aaaacctgca
     1201 tggctgccaa ccaataccac accggcacgg cacgcctgac cgaccaatat ttccgtactt
     1261 tgagtatgaa cctgaccgca cgcggcgaat ttgaaaccgg cccggtcacg cataactgga
     1321 gtacggcttt cgaccgcgtt atccgccagc ggaaaaccat aaacggcagc gggaacggca
     1381 acagcaagat agaagtgaag gcaaacgaaa atattgcgaa tcaattggca tcgttcaaag
     1441 ccgactatcc caattcttgg gcgaactcgg ccaacttgga tgccaatatc aaagtcaaca
     1501 gcttggcttt gtccgatact ttgggcttcg ttgataacaa ataccccctg actttgggcg
     1561 gacgtttcca agcggttgaa tacactgata agaagaaatc acagagcggc gatgccaaac
     1621 gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtc gtgtgcggca
     1681 actatatgga agacttggag cgtgccgata tcaaaaccga tgattccggc gaaacgacaa
     1741 tggcgaagcc gcgcgtcagc cgccagtttg aaatcggcgt gcgtaaaaac tggggcgatt
     1801 ttgtaaccac gttgaatgcg ttccaaatca aacgcccggg ctactggcgc ggcaatacca
     1861 aaaaaggtac tgattttgca gcatataaag cagcaggagg cgcagccggc gacgagcaag
     1921 gtatggaacg caatcgcggt attgagttca atgcctatgc caacctgttg aacaaaaccc
     1981 tgcgcccgac tttcggcgtg atgtatctgc aatcgtccgt gaagaattat ccgaatgcgc
     2041 gcgatatgct ggttaacggc gtacaagtcg ccaacccgcg cgtgattgtc aaaacaggcg
     2101 ttgaacggga tacaccgttt gccaaaggtt tgagcttgaa cggcaatgtt tcgtatttcg
     2161 gcaagtctta tcaagacacg caaaaacaat acgccttccc gtcctatacc ttggttgacg
     2221 taggcgcgcg ctacaaaacc aagctgggca aaaacaccct gaccgtcagc agctcggtag
     2281 aaaacctgtt caacaaaaac tactggcagg tacagcgcgg acaatttgac cgtagtttcg
     2341 ccgtcgtcgg tctgccgcgt acttactggc tgaaggcgga attggatttc taagctgcct
     2401 ccgtttccga aaatgccgtc cgaacaggtt tcagacggca tttcttttaa agaaatatgg
     2461 cgggatatgc ttaaataatg ctattctagt acctttgaaa cctgcttgtt tgaagtgatg
     2521 aaaaaacctt tgatttcagt tgcggcagta ttgctcggcg ttgctttggg tacaccttat
     2581 tatttgggtg tcaaagcaga agaaagtctg acgcagcagc aaaaaatatt gcagaaaacg
     2641 ggctttttga ccgtcgaatc gcaccagtat gatcgaggct ggtttacctc tacggaaacg
     2701 acggtcatcc gtctgaaacc cgagttgctg cataatgcgc agaaatacct gccggataac
     2761 ttgaaaatag tgttggaaca gccggttacg ctggtaaacc atatcacgca cggccctttc
     2821 gccggcggat tcggcacgca ggcgcacatt gaaaccgagt tcaaatacgc gcctgaaacg
     2881 gaaaaagttt tggaacgctt ttttgggaaa caagttccgg tttcccttgc caataccgtt
     2941 tatttcaacg gcagcggtaa aatggaagtc agtgttcccg ctttcgatta tgaagaactg
     3001 tcgggcatca ggctgcactg ggaaggcctg acgggggaaa cggtttatca aaaaggtttc
     3061 aaaagctacc gcaacagcta tgatgcgccc ttgttcaaaa tcaagctggc agacaaaggc
     3121 gatgccgcgt ttgaaaaagc gcatttcgat tcggaaactt cagacggcat caatccgctt
     3181 gctttgggca gcagcaatct gactttggaa aaattttcgc tcgaatggaa agagggtgtc
     3241 gattacaacg tcaaattgaa cgaactggtc aacctcgtta ccgatttgca gatcggcgcg
     3301 tttatcaatc ccaacggcag catcgcacct tccaaaatcg aagtcggcaa gctggctttt
     3361 tcaaccaaga ccggggaatc gggcgcgttt atcgacagcg aagggcggtt ccgtttcgat
     3421 acgttggtgt acggcgatga aaaatacggc ccgctggaca tccatatcgc tgccgaacac
     3481 ctcgatgctt ctgccttaac cgtattgaaa cgcaagtttg cacaaatttc tgccaaaaaa
     3541 atgactgagg aacaaatccg caatgatttg attgcggcag tcaaaggcga tgcttccgga
     3601 ttatttaccc atgacccggt actaaatatc aaaattttcc gtttcaccct gcctcaggga
     3661 aaaattgatg tgggcggaaa aatcatgttt aaaggcatga agaaggaaga tttgaaccaa
     3721 ttgggactga tgttaaagaa aaccgaggca aacatcagaa tgagtattcc tcaaaaaatg
     3781 ttggaagatt tggcggtaag tcaggctgga aatattttca gtgtaaatgc cgaagatgag
     3841 gcggaagcca gagcaagcat tgccgatatt aatgaaacat tgcgcctgat ggtggacagt
     3901 acggtccaaa gtatggcaag ggaaaaatat cttactttag acggtaatca gattgatacg
     3961 gtcatttccc ttaaaaacaa cgccctgaag ttaaacggga aaacgctgca aaatgaaccc
     4021 gatcctgatt ttgacgaggg agatatggtt tccggccagc cgcattaatg ccgtttatgc
     4081 caaatgccgt ctgaaatgtt cagacggcat ttttgtcggt ctcgaaatag gatggatttt
     4141 catgtttcaa aaacaattga ttgtctctaa tccgatatca ggacacgctt ccaatgttta
     4201 gatatggaat tcggacggga taggggaact attgaaatcc agttgaaatc tgttattgga
     4261 aagcatggag gaaaaataaa aggctgtatt ttaatatagt attgataggc atgcttagct
     4321 tcaaggtatt caatagtgta ggtgtctgcc taactggaaa ataatcgtct tttcccttgc
     4381 aaccagttaa aattgacaga tggttaaaac acagccaaat aaaaatgccg cctgaagggt
     4441 tcagacggca tttgttcgga taaaccttat cggctgctta gagcatcagg tttatttttt
     4501 cttttgagga ggcaggtcgg tacatacacc caaagccact tccgcagcca taccgatgga
     4561 ttcgcccaag gtcgggtgcg ggtggatggt tttgccgatg tctgccgcgt cgcagcccat
     4621 ttcgatggca aggcagactt cgccgatcat gtcgccaccg ttcggaccga caatgccgcc
     4681 gccgatgatg cggccggttt cggcatcgaa aatcagcttg gtaaagccgt tgtcgcaacc
     4741 gttggcaatc gcacgaccgg aagccgccca tgggaagttg gctttggtga ttttgcggcc
     4801 tgatgctttg gcagacaatt cggtttcacc gacccatgcc acttcggggg aagtgtaggc
     4861 aacgccggga ataacgcgtg cgtcgaagta ggctttgtgg ccggcacagt tttcagcggc
     4921 aacgtgacct tcgtgaacgg ctttgtgtgc caacatcggt tgaccgacga tatcgccgat
     4981 agcgtagatg tgcggtacgt tggtgcgcat ttgtttgtca acttcgatga agccacgatc
     5041 ggtaacggca acaccggctt tttctgcgct gatgagtttg ccgttaggcg cacggcctgc
     5101 cgcgaccagt acggcatcgt aacgttgcgg ctctttaggt gcatttgcac cttcaaaggt
     5161 aacgtaaacg ccgtcttctt tcggctcgac tgcaacggtt ttggtgttga ccataatgtt
     5221 gtcaaaacgg tattcgtttt gtttttgcca tactttaacc aagtcgcggt ctgcgccttg
     5281 catcaggccg tccatcattt caaccacatc caggcgagaa cccagcgtgc tgtaaaccgt
     5341 acccatctcg aggccgataa tgccgccgcc gataatcagc agtttgcccg gtacttcttt
     5401 cagtgccagt gcgccgctgg aatcgatgat gcgcggatct tcaggaatga aaggcagttt
     5461 ggttacgcgg ctgcctgctg caatgataca gtttttgaag gcaacgattt ttttctcgcc
     5521 ggtaggggct gcctgttcgt acgcgtcgcc ggcagtcagc gacacttcca agtggtgagg
     5581 atccaagaat tgcccgtcgc cttggataac gtccacttta cggcttttcg ccatacctgc
     5641 caaaccaccc gtgaggcggg aaaccacgcc gtctttatag gcgcgaagca tatcgatgtc
     5701 gagttccggt tcggggtatt tgataccgtt ggcagccaag tggcgcactt cgtcgataac
     5761 ggcggcattg tgcaacaagg ctttggaagg gatacagccg acgttcaggc aaacgccgcc
     5821 caaagttttg taacgctcga cgatggcgac tttcaagcct tcatcggcag cggcaaatgc
     5881 agcggagtaa ccgccgggac cgccgcccaa tacgaccacg tcgtactcgg catcggcagc
     5941 actgccgaat tgcgcggctt gcggagcagg agcggcagct ttgggtgctt cttgcgccgg
     6001 ggcggcagcc gcttcggctt taggggcggc agccgcacct tcggcttcga cgacgacaat
     6061 caaaccgcct tcggagattt tgtcgccgac tttgactttc acttctttga tcacgcccgc
     6121 aacttcggca ggcacgtcca tcgtcgcttt gtcggtttcc aaggtaatca gggtgtcgtc
     6181 aacggcgatg gtgtcgccaa cgttcacttc aaccgcgata atatctacat tttcgtgtcc
     6241 gccaatgtcg ggcactttca attcaactaa agccattata ttgacctttc aacttagtta
     6301 atttctatca actctttagc aataatcact tcttcaggaa tacagccaca atcttcagct
     6361 ctgattgtta cttccttctc atctgggttt tctggatgtt caacctcata ttcaaaaata
     6421 gaatattgac caaacaaatc tctatttaaa acatatatat agccattttc gatgccggag
     6481 cttgttgcaa atttcttggc aatttccttg tctgttgtcg tagatatata acatccgtca
     6541 tatagatctg tttcaatttg atgggcgtaa actgcattct tcacacttgg accatgtgta
     6601 gctttaccat catatttaaa cttaccatca taacgaattg caacttcagc tttattacct
     6661 ttaggtttta actgtccatt attttgctca tcttgttggc aactaatgcc tctatataaa
     6721 taagaaattt cccataagtt attaatttaa agtttgaatt taacaccatc actcatcaat
     6781 ctcaagcaat ccaaacatta tcagactgct tgagagggat gttttatttt ataaggtaat
     6841 gcggcggaag tctttcaaca ggttcgccag gaatacggtg aagcgcatac cggcggcacc
     6901 gtcgatgacg cggtggtcga aggacaggct caacgggcac atcaggcgcg gggcaaactc
     6961 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatag cgacttcggg
     7021 agcgttcaca atcggcgtga aacctgtgcc gccgatgccg cccaagctgg aaatggtaaa
     7081 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tggacaattc
     7141 ggtcaattct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     7201 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     7261 gttgtcgccg tccaaagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     7321 tttgatgatg aacgccaacg gggacagttt cacgccttcg cgttcccatt ctttgttcag
     7381 ctgtttgcgg aattcttcca gctcggtcat atccgcttct tcgtgtacgg taacgtgcgg
     7441 aatcaccacc cagttgcggg acaggttttg accggagatt ttcttaatgc gggacaattc
     7501 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     7561 accgccgccc aaagatgcgc cggctgcggc aggttttgcc gcgccgccct gcattacgga
     7621 tttcacaaag gctttgatgt cgtctcccat gatacggcct ttcaagccgc tacctttgac
     7681 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     7741 tttggcgaaa gcggcttcat cgattttggc agcggcaggc gaggcagatg taggcacggc
     7801 ggcgggtgcg gcagcttgag cggcaggcgc aggagctgct gcggcagagc cggcggtttc
     7861 cacttcgata atggcagagc cttcggacac tttgtcgccg acttttaaga atacggcttt
     7921 aacgacaccg gcagcggtac aaggtacgtc cattgtcgct ttatcggttt ccaaagtaat
     7981 cagcgtgtcg tcttcggcaa cggtgtcgcc aactttgatt tcaacggcga ttacatctac
     8041 atcggtatgg ccgccgatat cgggaacggc tacttgaacg gttgcaccgc ctgcggcagc
     8101 gggtgcaggt tgtgcttcgg cggcagcagc cggggcggct tcggcagcgg cggcaccggt
     8161 ttcaacggtc aaaattacgc cgccttcgga gattttgtcg ccgactttga cttttacttc
     8221 tttcacgaca ccggccgcat cggcaggcac atccatcgtg gctttgtcgg tttccagtgt
     8281 aatcagggtg tcgtcaacgg cgatggtgtc gcccgcttta acttctacgg cgatgatgtc
     8341 gacgctttcg tgaccgccga tatcggggac tttgatttct acgatactca tttgagttcc
     8401 tttaatcttt ttcagttgga tgccgtctga aagctgcttc aggcggcatc ggtacggttt
     8461 gtctgttcaa accattcggg cattcggttt tcagacggcc tgatgttggc atgaaacaaa
     8521 caggccgtct gaaacatcaa tcagcgtttc cagctagggg ctgaatcggc tttgatgccg
     8581 tatttctcaa tggcttgttg aacggtttct ttgctgactt tgccttgttc cgccaatgcg
     8641 gccaatgctg ccacggcaac gttgtagcgg tccacttcaa agaagcggcg caggttggcg
     8701 cggctgtcgg aacggccgaa accgtcagtg cccaagacat ggtagtcgtt cgggatgtac
     8761 gcgcggatac ggtcggcata gctgcggata tagtcggtag cggcgataac cggaccgtca
     8821 tgaccttgca gttgggaagt aacgaaaggc actttttcag cttccagcgg atgcaggcgg
     8881 ttgaagcgtt cggtttcgat ggcgtcgcgg tgcagcaggt tgaaggacgg gcaagaccag
     8941 atgtctgctt ccacgccgaa gtcggctttc agcagctcgg caccggcaat cacttcttgc
     9001 agaatcgtac cggaacccat caattggact ttcttgtcgc ctttgccgcc ggctttcagc
     9061 aggtacatac ctttgaggat gtcttgttcc gcaccttccg gcatatcggg atgggtgtag
     9121 ttctcgttca tcagggtgat gtagtagaac acgtcttcat tattggcata catacggcgc
     9181 agaccgtctt gtacgatgac ggccacttcg tattggaagg tcgggtcata agatacgcag
     9241 ttcggaatca ggtcggcctg gatgtggctg tggccgtctt cgtgttgcag accttcgccg
     9301 ttcagcgtcg tacggccggc agtaccgccc agcaggaagc cgcgcgcgtg catatcgccc
     9361 gccgcccaag ccaagtcgcc gatacgttgg aaaccgaaca tggagtagta gatgtagaac
     9421 ggaatcatcg cgaagtcgct gttggcgtag ctggtcgcag ccgcaatcca gtcggccatc
     9481 gcgcccggtt cgttaatacc ttcttgcaag atttgaccgt cgacggattc tttgtagaac
     9541 atcagttggt ctttatcttg aggcgtgtat tgttggcctt tgggattcca aataccgtat
     9601 tggcggaaca taccttccat gccgaaagta cggctttcgt cgggaacgat aggtacgacg
     9661 cgtttgccga tttttttgtc tttcagcaaa gtggacagga tgcggacgaa tgccatcgtg
     9721 gtcgagaact cgcgttcacc gctggatttg agttgtgcgt cgaatgccga cagctcgggc
     9781 acttccaata cttcctgcgt cggtttgcgt tgcggcaggt agccgcccaa agcatcgcgg
     9841 cgtgcgtgca ggtatttgta ttcttccgta tcgggggcaa aagtcaggta aggcagatcg
     9901 ccgctttcga tttgttcgtc ggtaaccgga atgtcaaagc ggtcgcggaa ttgtttcagg
     9961 gacgctttgt ccattttttt ggcttggtgg gcaacgttct gaccttcgcc ggatgcgccc
    10021 ataccgtaac ctttaatggt tttcgccaag ataacggtag gtttgccgtc ggcatggttc
    10081 gctgcgcggt cgtaggcgtt gtacactttt tgaggatcgt ggccgccgcg gttcaatgcc
    10141 cagagttgct catcggtcat atcggcaacc aatgctttca gttcgggcgt attgaagaag
    10201 tgttcgcgca catacgcgcc gtctttggat ttgtaagttt ggtagtcgcc gtccaaacat
    10261 tcttccatac gttggcgcag gataccgtct ttgtctttcg ccaagaggcg gtcccaacgg
    10321 cggccccaaa tgactttgac gacattccag ccggcaccgg caaagttgcc ttccaattct
    10381 tggatgattt tgccgttgcc gcgcaccgga ccgtccaagc gttgcaggtt gcagttgatg
    10441 acgaaaatca ggttgtccaa gccttcgcgt gcagccagcg cgattgcacc ttgggattcg
    10501 ggttcgtcca tttcgccgtc gccgcagaaa caccatactt tacggccttt ggtttttgcc
    10561 aagccgcgtg attccaagta tttcaggaaa cgcgcctgat aaatcgccat gatggggccc
    10621 aagcccatgg atacggtcgg gaactgccag aagtcgggca agaggtgggg gtgtggatag
    10681 gaaggcagac cgtgtccgtc cacttcttgg cggaagttgt tcagctggtc ttcggtcaga
    10741 cggccttcga cgaatgcgcg tgcatagatg cccggggcaa cgtgaccttg gaagaagacc
    10801 aaatcgcctt cttcaccttc acctttggct ttccagaagt ggttgaaacc gacttcgtac
    10861 atggtggcgg cagattggaa agatgcgatg tgcccaccca gttccaaatc tttcttgccg
    10921 gcgcgcaata cgatggcggc ggcgttccag cgtacgaatg cgcgaatgcg gtgttcgatg
    10981 ttttggtcgc ccggaatgcc tttttcgttt tcaaccgaaa cggtattcaa atacggggtg
    11041 gtcgtgccgt ggggcatacg tacgcctttg tcgcggcagt atttgagcag gttttccaag
    11101 aggtattgcg cgcgttcgcc gccttcatat tcgaggacgg agcttaacgc gtccagccac
    11161 tcttgggttt cgatagggtc aacatcgtgt aattgggtgg acatagtatc tatcctttat
    11221 gttgagtgtt taatcggaag ggagggtgta gccaacccct ttattttcgt ttgtgaaaaa
    11281 taataagcaa aaacgaaaat ttctaaatca aaccgaaaca aaataattta agtttaatgt
    11341 tttcaaaaaa ttataaaagt cttggaagca agggaaaact tgaaaaaaaa aatgttcggt
    11401 ctgctccgca cgaaatccta tcagcaatcg ggtctgctgg caaataacca tgcttttaaa
    11461 agcaggtttt atactgaagt tggcgtttgg taaggacagg tctttttgat tgcgaaaaca
    11521 ttctgtttaa ttttggttat attttttcgg aattgtggga tatcgaatcg ttatgtctct
    11581 gtcggcaatt ttcctattgc agaaaatcta tacacaagct ttgtggacaa ccgtgttgat
    11641 aagccacggc ttgtgttaaa aaagtcttac gcaacaaatg tacgcttatt ttgattaaaa
    11701 attaggcaaa tttaaatcta taaaaatcat gtaattatgt tcaatatgat gtttctggtc
    11761 atgtttttgc cgtctgttat ggggtttgtc ccgcgtttgg ggcagggggt gaattttgga
    11821 ggttggtgca aaacctgctt ttttcgcgtt acaatctgca cccgatttac ttttgaatct
    11881 gcctgtttgg aaaaatactt ttatgaaagc cagccaattc tttatctcta ctttaaaaga
    11941 agcccctgcc gaagccgcgt ttgccagcca caagctgatg attcgcgccg gtctgattaa
    12001 agccaacgcg tccggtcttt atacttggat gccgatgggg ctgcgcgtgt tacgcaaagt
    12061 cgaaaacgtc gtgcgcgagg aaatggcgcg cgcgggcagc gtggagctgc tgatgccggt
    12121 ggtgcagcct gccgaactgt ggcaggaatc cggccgctgg gagttttacg gtaaagaact
    12181 gctgcgcctg aaagaccgcc acgaacgcga tttctgcatg ggcccgacct gcgaggaagt
    12241 catcgccgac atcgtgcgca aagaaatcaa cagctacaaa caactgccga aaaattttta
    12301 ccacatccaa accaaattcc gcgacgaagt gcgcccgcgt ttcggcgtga tgcgcgcgcg
    12361 cgaatttgtg atgaaagatg cttattcctt ccacgccgac tacgcctcgc ttcaggcgac
    12421 ctatgatgcc atgtatgacg cttactgccg catctttacc cgtctgggtt tggcgttccg
    12481 ccccgtcgcc gcagacaccg gcagcatcgg cggtacgggt tcgcacgagt ttcaagtgtt
    12541 ggcggaaagc ggcgaagatg tgattgcata cagcgacact tccgattacg ccgccaatat
    12601 cgagttggca ccgaccttgc cgcttaaagg tgaacgtgcc gccgctcagg ctgtgttgac
    12661 caaagtacat acaccaaacg tcaaaaccat tgagtctttg gttgaattcc tgaatattcc
    12721 ggttgaacaa accctcaaat ccatcgtggt tgaaggcgaa aacgaaggcg aactcgtcct
    12781 actgctgttg cgtggcgacc atgagtttaa cgacatcaag gcagaaaaac tggcgggcgt
    12841 aaaatcgcca ctgactatgg caagccctgc cgcgattgtt gaacaattcg gcgcaaacgg
    12901 cggttcgctc ggccccgtcg gcttcacagg caaagtctat gccgatttcg ctaccgaaaa
    12961 aggcgcggac tgggttatcg gcgcaaacga agacgactac cactataccg gcttcaactt
    13021 cggccgcgat gccgccgagc ctgaattcgt cgatttgcgt aatgtggtcg aaggcgacga
    13081 aagccccgac gggcaaggcc gtctgaaact ggcgcgcggc atcgaagtcg gacatgtctt
    13141 ccagttgcgc ggcaaatata cccaagctat gaacgtcagc ttcctcgaca ataacggcaa
    13201 atcgcaaatc atggaaatgg gctgctacgg catcggcatc acccgcgtcg ttgccgccgc
    13261 catcgagcag aataacgacg aaaaaggcat catttggacc aaagcgatgg cgccgtttga
    13321 agtcgtcatc gtgccgatga actacaaaaa atccgacact gtgcgtgaag ccgccgacag
    13381 aatctatgcc gaattgctgg cggcaggcgc ggatgtgctg ctggacgacc gcgacgaacg
    13441 cgcaggcgta ttgctgaacg attccgagct tctcggtatc ccgcaccgca tcgtcatcgg
    13501 cgaccgcgcc ttgaaagaag gcaatgtcga atacgccgaa cgccgcgaca acgaagcgca
    13561 ggcagttgca atcggagaaa ttgttgcgcg tgtaacagct tcattaaatg cgtaagtctg
    13621 gtcaaatgcc gtctgaaaat aatttcagac ggcattttta ttttggggga aagtgaagtg
    13681 gcttcagtat ttttagaagg agaagcagtc gaggtcggca atattttctg tatcggcagg
    13741 aactatgccg cacatgtcga agagttaaaa aacgaaatac cttcagaacc tgtggtgttt
    13801 atgaagccgt caggcagcat tttgaacagc ggcggtacaa tcttgctgcc cgagttcagt
    13861 agggatgtgc agtttgagtg cgaactagtg ttgctgattg gccgggattc agacggcacg
    13921 ggcgagggtg aagatatttt gggatgcgtg gcggggtacg gcgtgggttt ggatttgacc
    13981 gcgcgggaca tccaatgccg tctgaaggcg caggggctgc cctggctgaa ggcaaaaggg
    14041 ttcaggcatt ctgcgtgcgt gtcggatttt gcggcggcag acagaatcgg caatcaagat
    14101 aaggtgttgt tttcattaaa acaaaacggc gtattgaaac agcgcgggga gactggtttg
    14161 atgatttacc cgattcggga aattttacac aaactggcgg cggattacgg tttggggaaa
    14221 ggggatttgg tgtttaccgg aacgccctcc ggcgtgggcg caatcggtgc gggggacaat
    14281 ttggcattgg agctggacgg cttggtgtgc gcctctttca cgatagggtg ttaaccgcgc
    14341 cgcttaacct tcacgcccgt tgaaacattc cgccgggccg ccctcgagaa aaccgtgcag
    14401 gatggcttgc gccgccactt ggtcgagcac cgatttgcgt tttttgccca agacctgtgc
    14461 ttccgaaagc aggctttcgg catagacgga cgacagccgt tcgtcaaccc aatagacggg
    14521 gagattgaac ctgccgttca gcctgcgtcc aaacttgcgc gacaggtgcg tcatttcatg
    14581 ttccgtgccg tcggcatgca cgggcagtcc gacgacaaaa taacgcggct gccattcttg
    14641 aaccagcttg gcgattgccg cgaatttttc atcgttgctg ccgccggtaa cggtggccag
    14701 cggatgggac agccccaatt ccgcatcgcc ctgcgccacg ccgatgcgcg cttcgccgaa
    14761 gtcgaatgcc agtgccgttc cttttggaat tttatgcatg gccggctccc gaaaacaggg
    14821 cgagcgggtc gatgccgagt ttggcgaatg cggcggcgta acggtgttcg taggggatgt
    14881 cgaacaggat gtgttcgtcg gcgggaacgg tcagccacgc attgtcggca agttcgcgtt
    14941 cgagctgccc tttgctccag ctcgaatagc ctatgctgat caaggctttg tcaaccgcgc
    15001 cttcgcgtga aatgttttca agcacatcgc gggaagaggt cagcgcgatg ccgtctgaaa
    15061 cgccgatgct gctttgccag ttgccgatcg gagtgtggac gacgtaaccg cgctcgacct
    15121 gcaccggccc gcccatcatc acgctgtcgt gctgcatccg catggggatg tttttgccgg
    15181 tggcggaaaa aatcatgtcc atcgtaatcg gagagggttt gttgatggcg ataccgagtg
    15241 cgccgtcttc atcgtgtttg cagatataga cgaccgattg tgaaaaaaac gcgtcttcca
    15301 tatcgggcat ggcgacgaga aagtggttgg acaggttcat atcagcgttt gtcgggattt
    15361 tttatcgggt tggaaataat gcaggcgccg gcaagcagtt tgccggaagt atcggcttgc
    15421 accccgtcgg tttgcggtat gccgctgccg aaacaggaaa ccgcgcttaa ggaatttttc
    15481 ggcgatcctt ggacgatttt atcgctgtaa accaaatagg cgaaggcttt gcgtttgggg
    15541 tcgtaataac ggacaatctg ccggctcttg aacgcgaagc ccgtaccgcg cttgaaaact
    15601 tctttcggtt tgcgcacggc ggtttcgtca aaagaaatcg aagatgccgt ctgaacgcac
    15661 gaaaccgatg cgtcggacgc gtcctcttcc aaattgacca tttccttcaa gccgcctttt
    15721 tttgcatacg aaatataaca ggcaacccct tgaacgtcgg gatcgtcgaa tccttccact
    15781 tcgatacggt cgtttttgcc caacatgttg aaaacggtac ttgcccgtcc gattttatcg
    15841 gtttcgccgc tgccgcaggc agtcggcagg acggcggcag acagcagtag cagtctgttc
    15901 atcaaaattt ccttatcggt tgaatccccg ttttccggaa ggcggcaggg tttgctttgt
    15961 ttggaggggc gcacgctccc aaatcgggca acatacggta cgcagcttac atgtgctacc
    16021 ctgtgtgtcg gaacacaata tccgttgcag cattgaaaat atgccgcaca atataaggct
    16081 gtttgagccg attgcaagta tccgcgcaaa gaaggtgcaa gat
//
LOCUS       DAWTCI010000047        16016 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWTCI010000047 DAWTCI010000000
VERSION     DAWTCI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16016
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.047"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            344..2728
                     /gene="ppsA"
                     /locus_tag="WM481_001412"
     CDS             344..2728
                     /gene="ppsA"
                     /locus_tag="WM481_001412"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFA9080713.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLNERISAALAKLDVEDVSELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWDKMVTDADGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKKAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYNQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVEVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRYAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEIIRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVAESFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVGEGIESVSLNPDTVIETWLYLANELNK"
     gene            2949..4208
                     /gene="rho"
                     /locus_tag="WM481_001413"
     CDS             2949..4208
                     /gene="rho"
                     /locus_tag="WM481_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFA9080714.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKIGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPASGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            4824..6128
                     /locus_tag="WM481_001414"
     CDS             4824..6128
                     /locus_tag="WM481_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFA9080715.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAARMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFIMA
                     AYSGLMSWAILKAVGKICGGLRVGKDVEREGLDLNIHGEHVE"
     gene            complement(6222..7517)
                     /locus_tag="WM481_001415"
     CDS             complement(6222..7517)
                     /locus_tag="WM481_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFA9080716.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKAETCIIGGGLSG
                     LCTALPLAEHGHEAVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVASAAAEAGAQIFEQSPMTRIEP
                     YQNGWLVYTPEGSVECKNVVYAVNTYVGLNPIFRPLERKAIAVSTFIIATEPLGARTK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVRIEYSWGGECDITANLVPHFGRLTSNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(7644..8531)
                     /locus_tag="WM481_001416"
     CDS             complement(7644..8531)
                     /locus_tag="WM481_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9080717.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     NREAAEAYRQEKLAAEKAN"
     gene            complement(8531..9496)
                     /locus_tag="WM481_001417"
     CDS             complement(8531..9496)
                     /locus_tag="WM481_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFA9080718.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISSA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIINNLLIKMGIIREPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDSRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(9512..10636)
                     /gene="potA"
                     /locus_tag="WM481_001418"
     CDS             complement(9512..10636)
                     /gene="potA"
                     /locus_tag="WM481_001418"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFA9080719.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLNIYQNEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIDARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIENHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(10914..11183)
                     /gene="rpsO"
                     /locus_tag="WM481_001419"
     CDS             complement(10914..11183)
                     /gene="rpsO"
                     /locus_tag="WM481_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFA9080720.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(11388..12323)
                     /gene="secF"
                     /locus_tag="WM481_001420"
     CDS             complement(11388..12323)
                     /gene="secF"
                     /locus_tag="WM481_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFA9080721.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVARGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKMGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVNNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHTVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIAKEAKQKEEIVV"
     gene            complement(12327..14180)
                     /gene="secD"
                     /locus_tag="WM481_001421"
     CDS             complement(12327..14180)
                     /gene="secD"
                     /locus_tag="WM481_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFA9080722.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAENSLTVPLQDAGDVQKALPQLLKLFPEATLNSEGSNIVLALSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPIIQQSGLDRIVVQLPGVQDTAKAKDIIGRTAT
                     LEVRMVEDDPAKLREALEGNVPSGYELLSSGGEHPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(14392..14658)
                     /gene="yajC"
                     /locus_tag="WM481_001422"
     CDS             complement(14392..14658)
                     /gene="yajC"
                     /locus_tag="WM481_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFA9080723.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKAGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            14789..15907
                     /locus_tag="WM481_001423"
     CDS             14789..15907
                     /locus_tag="WM481_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFA9080724.1"
                     /translation="MPLTRLFLKLYALLRLFLGKNAQTAWIFHPACTGHDPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTVEAEEISDTRLALVRSSKYLNRLESCLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMKRKAWHAFCAARPPGHHAKSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NGMPDGGNMVHLPLPSGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASSCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLNPTKKRFAKPKTGQVRQPTQSDRYDINAV"
BASE COUNT         4035 a         4371 c         4003 g         3607 t
ORIGIN      
        1 aacatatctg ctaatctggt acagacccaa aaacaaatca tattttaaaa caaattatct
       61 caaaatcaga tttctttaaa ataaaattcc aaaaaaaaga aacaggaaaa atacggcata
      121 aaacattaac ctattcaaaa ccatctgttt tacaaaggaa atacccgaaa aaaacaatat
      181 gcctgccatc ggtttcaaac cgctacacag ccggaacccg ctatttttca gacggcgcag
      241 cggttgtccg gacagataaa aacgtgacag cggcggaacg aatcgcttaa aataagcaag
      301 cggatttgtt tcttttttaa catattttgg attggacaca caaatggccg acaactacgt
      361 aatctggttt gaaaacctgc gtatgacaga tgttgaacgc gtgggcggta aaaacgcctc
      421 gctgggcgaa atgatcagtc aactgaccga aaaaggcgtt cgcgtccccg gtggctttgc
      481 caccacagcc gatgcctacc gcgcatttct cgcacacaac ggtctgaacg aacgcatttc
      541 cgccgctctg gcaaaattgg atgtcgaaga cgtttccgaa ctggcacgcg tcggcaaaga
      601 aatccgccaa tggattttgg acacgccctt ccccgaacag ctcgatgccg aaatcgaagc
      661 ggcatgggac aaaatggtta ccgatgccga cggcgcggac atttccgttg ccgtacgctc
      721 ttccgcaacc gccgaagacc tgcccgacgc atcatttgcc ggccaacagg aaaccttcct
      781 gaatatcaat ggcttggata acgttaaaaa agcgatgcgc cacgttttcg cctccctgta
      841 caacgaccgc gccatttcct accgcgtcca caaaggcttc gaacacgaca tcgtcgccct
      901 ttccgccggc gttcaacgca tggtgcgttc cgacagcggc gcatcaggtg tgatgttcac
      961 cctcgacacc gaatccggct acaaccaagt cgtctttgtt acctcctctt acggtctggg
     1021 cgaaaacgtc gtacaaggcg cggtcaaccc ggacgaattt tatgtgttca aacccacgct
     1081 caaagcaggc aaacccgcca tcctgcgcaa gaccatgggt tcgaaacaca tcaaaatgat
     1141 tttcaccgac aaagccgaag ccggcaaatc ggtaaccaac gtcgaagtcc ccgaagaaga
     1201 ccgcaaccgc ttctccatta ccgacgaaga aatcaccgag ttggcacact acgcgctgac
     1261 catcgaaaaa cactacggcc gcccgatgga tatcgaatgg ggacgcgacg gcctggacgg
     1321 caaactctac atcctgcaag cccgccccga aaccgtcaaa tcccaagaag aaggcagccg
     1381 caacctgcgc cgctacgcca tcaacggcga aaaaaccgtc ttgtgcgaag gtcgtgccat
     1441 cggtcaaaaa gtcggtcagg gcaaggtgcg cctgattaaa gatgcttccg agatggattc
     1501 cgtcgaagcc ggcgacgtac tggttaccga catgaccgat ccggattggg aacccgtgat
     1561 gaaacgtgct tccgccatcg tgaccaaccg cggcggacgc acctgccacg ccgccatcat
     1621 cgcgcgcgaa ctgggtattc ctgccgtcgt cggctgcggc aatgcgaccg aactcttgga
     1681 aaacggtcag gaagttaccg tatcctgtgc cgaaggcgat accggcttta tctatgccgg
     1741 cctgctggac gtacaaatta ccgatgtcgc cttggacaat atgccaaaag cacccgtcaa
     1801 agtcatgatg aacgtcggca atcccgaact cgcattcagc ttcgccaatc tgcccagcga
     1861 aggcatcggc ttggcgcgta tggaatttat catcaaccgc caaatcggca tccacccgaa
     1921 agccttgttg gaatttgaca aacaagacga cgaactgaaa gcggaaatta tccgccgcat
     1981 cgccggttac gcatcccctg tcgacttcta cgtcgataaa atcgccgaag gcgtggcaac
     2041 gcttgccgca tcggtttatc cgcgtaaaac catcgtccgt atgtccgact tcaaatccaa
     2101 cgaatacgcc aacctggtcg gcggcagcgt atacgaaccg catgaagaaa acccgatgtt
     2161 gggcttccgt ggtgcggcgc gttatgttgc cgagagcttc aaagactgtt tcgccttgga
     2221 atgcaaagcc ttgaaacgcg ttcgtgacga aatgggtctg accaacgtcg aaatcatgat
     2281 tccgttcgtc cgcactttgg gcgaagccga agcagtggtt aaggcattga aagaaaacgg
     2341 cttggaacgc ggcaaaaacg gcctgcgcct gattatgatg tgcgagctgc cgagcaacgc
     2401 ggtattggcg gaacaattcc tgcaatactt cgacggcttc tccatcggct cgaacgacat
     2461 gacccagctg accctcggcc tcgaccgaga cagcggcttg gtgtccgaat cgtttgacga
     2521 acgcaaccct gccgtcaaag tgatgctgca tcttgccatc tccgcctgcc gcaagcagaa
     2581 caaatatgtc ggtatctgcg gtcaaggtcc gtccgaccat ccggactttg ccaaatggct
     2641 ggttggagaa ggcatcgaaa gcgtgtctct gaacccggat accgtcatcg aaacttggct
     2701 gtatttggcg aatgaattga acaaataatc aattcccata cccccaagcc tgaaaagcgc
     2761 gggggtattt tttccaagca gctccgttca gacggcattt cctgtcgatg ccccgtccgc
     2821 gataatattt gacacccacg cgccgactgc ctacaattcc cccctctccg agcaaccggc
     2881 aacagtcagc ttcttctttc agacggcatc cacccgtctt ttcctttcta ttctatatat
     2941 cattgattat gcacgtctcc gaattacaaa ccctgcacat ttccaaactc ttagaattgg
     3001 cggaagaaca cggcatcgaa aacgccaacc gattccgcaa acaagacctc gtatttgcca
     3061 tcgtccgcca gatgatgaaa aaaggcgagg gttttacctg ctccggcacg ctcgaaatcc
     3121 tgcccgacgg cttcggcttc ctccgcagcg cggacacgtc ctatcttgcc ggccccgacg
     3181 acatctatgt ctcgcccact caaatccgcc gcttcaacct gcatacgggc gacaccatcg
     3241 aaggcagcgt gcgcgtcccc aaagacaacg aacgctattt tgccttggtc agactggatt
     3301 ccatcaatgg cgaccacccg gaagtatgcc gccataaaat cctgtttgaa aacctgaccc
     3361 cgctgtttcc gaccgaacag ttgaagctgg aacgcgactt aaagtccgaa gaaaacctga
     3421 ccggacgcgc catcgacctg atttcgccca tcggcaaagg tcagcgcgcc ctcttggttg
     3481 ccccgcccaa aatcggtaaa accgtgatgc tgcaaaacat tgcccacgcc gttaccgcaa
     3541 actatcctga agtcgaactc atcgtcctct tgattgacga acgccccgaa gaagtaaccg
     3601 aaatgagccg ttccgtccga ggtgaagtag tctcctccac ctttgacgaa ccggcgcaac
     3661 gccacgtcca agttgccgaa atggtgcttg aaaaagccaa gcgtatggtg gaacacaaaa
     3721 aagacgtggt catcctgctg gattcgatta cccgccttgc ccgcgcctac aataccgtcg
     3781 tccccgcctc gggcaaaatc ctgaccggcg gtgtcgatgc caacgcgctg caccgcccca
     3841 aacgtttctt cggtgcggcg cgcaacgtgg aagaaggcgg ttcgctgacc attatcgcca
     3901 ccgcattggt tgaaaccggc agccgtatgg acgatgtgat ttacgaagaa ttcaaaggta
     3961 ccggcaatat ggaattgcac ctcgaccgcc gtatggcgga aaaacgcctc ttccccgcca
     4021 tcaacatcaa caaatccggc acgcgccgcg aagagctgct tgtgccgaac gaccagttgc
     4081 aacgtatgtg gttactgcgc aagttcctgc acccgatgga cgaaatcgaa gctaccgagt
     4141 tcttaaacgg aaaaatcaaa gcatctaaaa ataatgatga tttctttgaa ctgatgcgcg
     4201 gaaaataaac gcgccgcccg cattaatgcg aaatgccgtc tgaagcctga aaatcgggtt
     4261 tcagacgaca ttttcattcg cacggccggc gcagctcccc ccccccgcta acggcgcaac
     4321 cgccggcagt gtccgtgtcc gcctgccgaa ggcgcggcct ttgcaaaacc ggctcgaacg
     4381 catccctacc ggcaatgaaa accgatgcgg gaacttgatt caaggttgcg ccgaaccgcg
     4441 cccatttttg tataaatttt attttttatt tccaattaga aatttttaat atattaatca
     4501 ataaattaat tttataaaat aaaaatattg tcaacaatct tttgacttat cgcccaaacc
     4561 tctgtatatt ttcctacagt aaattgttga caatccatac gcccgcatat gcgccgccta
     4621 aggataaatc ctcccgccgg acaacggggg caagggatag gatgcgatat ttccatattc
     4681 aaacaaggga tttgtttcac gcacagggcg gcacatcggc aaaatccccg cgccggtccg
     4741 gcagggcttg cgcccatccc ggacaagccc cgaccccgcc tttccgaaag acgggcccaa
     4801 ccattaagga aactttaatc aaaatgaaaa aacacatatg ggcggcatct ttgctgccgg
     4861 catccctatc ggcagaacct ttaaactggt ggaaacctta ttccgccgtc aattcgggcg
     4921 ataccgcctg ggtgatgact gcggctgcct tggtactgtt gatgacgctt cccgggctgg
     4981 ctttattcta cggcggtatg gtgcggaaaa aaaacctgct ctcgacgatg atgcacagct
     5041 tttccatcgc gacattggtg ggcatccttt gggtcgccgt cggctattct ttggcgttca
     5101 cgccgggaaa tgcctttatc ggcggtttgg ggcgcgtatt tttaagcggg atgcagatag
     5161 acgctgccgc acggatgctg accgtgtcgc ccaatgcgcc gactgttccc gaaccggtat
     5221 ttatgttttt tcagatgacg tttgccatta tttcgactgc cattattacc ggcgcgtttg
     5281 ccgaacgaat gaaatattcg gcaatgatgc tgttttcggg catatggttt ttattggttt
     5341 atgtgccggg cgcgcattgg gtgtggggcg gcggctttat gagcaagggc ggcgtattgg
     5401 attatgccgg cggtacggtg gtgcacatca atgccggtat cgcgggactc gtcgccgcct
     5461 tggttttggg caggcgcata ggctacgggc gcgaggcgat gcctccgcac aatatggcga
     5521 tgacgctgat tggcgcggca atgttgtggt tcggctggtt cggctttaac gctggttcgg
     5581 cgcttgcggc agacgcggcg gcggggatgg cgatggcggt aacgcaggtg tcggccgtat
     5641 tcggcgcggc aggctggctt gcctgcgaga aaatagcggg acacaaacct tctgctttgg
     5701 ggctggcttc cggcgcagtt tccgggctgg tcggcatcac ccctgccgcc ggctttaccg
     5761 gtccgtcggg cgcggccgcc atcggtatat tgactgccgc cgcgtgcttt gtgtccgtca
     5821 ccgtcgtcaa acacaaattg cgttacgatg attctttgga cgctttcggc atacacggat
     5881 tcggcgggct ggtaggcgga atattgaccg gcatcttttt cgacaaccgc attttcggtg
     5941 gggatgcggc agtttggcag cagttgtgga tacaggtaaa agacgggttc attatggcgg
     6001 catacagcgg gctgatgagt tgggcgattt tgaaggctgt ggggaaaatc tgcggcggtc
     6061 tgcgcgtcgg caaggatgtc gaacgcgaag gtttggatct gaatatccac ggcgaacacg
     6121 tggaataagg acggctatgc cgtctgaagc ctgaaaatcg ggtttcagac ggcatttttc
     6181 acgtttgccg ccgatgggga taaacatact gcctgcgacg cttaacggct gtctttccac
     6241 tgataatatt tcgagccgag gaaagaaccg agtttgcgca aaaacggttg caggataata
     6301 ttcggctggc gcaactgttc aaacggtttc agacggcatt cgtcccctaa aattgcttcg
     6361 gcaaccgcca ggcctgcaat gcccgttatc gccatcccgt gcccggaata accttgcgta
     6421 taaaaaacat tcgaggtcaa acgtccgaaa tgcgggacaa ggttggcggt aatatcgcac
     6481 tccccgcccc acgaatattc gattctaaca tcggcaagct gcggaaaaac tttaagcata
     6541 tcttggcgga caagctcggt catacgcgca ggattgtcga taaattcgtt atccttaccg
     6601 ccgaaaagca gcctgccgtc cgcgctgagg cggtaataat ccaaaatatg gcggttgtcg
     6661 catactgcca tattgttgcg gataagccct tttgtgcgcg cgcccaaggg ttcggtggca
     6721 ataataaagg tgctgacggc aatcgccttg cgttccaaag gccggaatat cgggttcaaa
     6781 ccgacataag tattgacggc atagaccaca tttttacact cgacgctgcc ttcgggcgtg
     6841 taaaccagcc aaccgttttg atacggttcg atgcgcgtca tcggggattg ctcgaaaatc
     6901 tgcgcgccgg cttcggcagc ggcgctggca acacccaacg tgtaattgag cggatgaaga
     6961 tgcccggaca agggatcgaa ctgtgcgcct tggtacatat cgctgtcaag ctgctgtttc
     7021 aactcggctt tatcccaaag ttgataatga ctcgcaccgt aatgccgttg ggcgtgttca
     7081 tgccactgct gcaactcttc ccaatgctgc ggacggacgg caaccgtggc ataaccgcgc
     7141 tgccaatcgc aatcgatggc atgtttgcgg acgcgttcgt ccaccagttc gaccgcctgc
     7201 aaagactgtt gccaaaacca ttgcgcctgc tccaagccga cctgtttttc aatttccccc
     7261 ataccgcagg cgtagtcgct gataacctgc ccgccactcc tgccggacgc gccgaagccg
     7321 atacgtgcgg cttccaaaac gacggcttca tgtccgtgtt ccgccagcgg caatgcggta
     7381 cacaaaccgc tcaaaccgcc gccgataatg caggtttcgg ctttcagacg gcattggagt
     7441 ttcggataaa cagtatgcgg attaaccgaa ctaaaataat aagaaggcag atattcttga
     7501 aaatcagggc gaatcattgt gtttgcttta tcgggtatat tttcggacgg aatgatacag
     7561 gctgtcgggc catatcgtcc aaacagaaaa tcggttgaaa aaaaacaggc tgacccagtc
     7621 atgcggtcag cctgccttat taattaattt gctttctcgg cagccaattt ttcttggcgg
     7681 taggcttctg ccgcttctcg gttgcgcttg gttgcctgcc tcatcatcca ataattgacg
     7741 atgatgacca atgttccgat gatgccgatc aggatggttg ccaagacatt catctgagga
     7801 tcgagaccca acttgatctt ggagaaaata acctgcggca atgtggatga accggggccg
     7861 gagaggaatg aggtaatcac caaatcatcc aaagacaagg taatgccgag taggaagcct
     7921 gaagcaatgg caggggcaat caaaggcaaa gtgatgacaa aaaagatttt cagcggacgc
     7981 gcgcccaagt ccattgcggc ttcttcgagc gactggtcaa gctcgaccag ccgcgaacgg
     8041 atgacaacgg taatgtatgc catgcacagc gtcgtatgtc cgaggaagat ggtgaaaaag
     8101 ccgcgatcga agtagagatg ttgcaaccat tcgctgccct gcaaaaatat ctgcacctga
     8161 ataatcagca gcagcataga cagaccggta atcacgtcgg gcatgaccat gggcgcggaa
     8221 atcataccgg caaacagcgt gctgccgcga aagcgtttga tacgcgccat cgcatagccc
     8281 gccagcgtgc ccaaaacgac ggcggcaagc gaagacacaa cggcaatccg cagcgacagc
     8341 caagcggctt ccaaaatggt gtcgttttcc agcaatgcgc cgtaccactt ggtcgaaaag
     8401 ccgccccaaa cggtaacaag tttggattcg ttgaacgaat agatgaccaa aacaaccagc
     8461 gggatataca gaaacgccag cgatagtgcc aacatcagtt tcaagaacca agataatttg
     8521 gatttctgca ttatttggct ccttcttcca attcgcggtt ttcatagtgc tggaacaggg
     8581 caatcggtac aaccagtagc gcgaccatca cgacggcgac ggcagaagcc aacggccagt
     8641 tgttttgatc aaagaatgcc tgccacaata ctttaccgat catcaggttt tccgaaccgc
     8701 cgaccagctc gggaatgacg aactcaccga cagcggggac gaaaaccagc atcgaacctg
     8761 caataatgcc ggttttcgac aaaggcaggg taatcgtcaa gaacgatttg accggtcccg
     8821 cgcccaagtc ggaagccgct tcgagcaggc ggctgtccag tttcaccagc tgcgtgtaaa
     8881 gcggcagaat cataaacggc agataggcat aaaccattac caaatttaac gaaaacgcat
     8941 tatagaacaa atctaatggt tctctgataa tacccatctt aatcaataag ttgtttatga
     9001 tgccgttatg cccgagcaaa cccatccacg catagacacg cagcaggaac gatgtccaaa
     9061 aaggtaacat gatggcaagc agcaaaccat tgcggacgga aggattggca cgggaaatcg
     9121 cataggcggt cgggtaaccg accaacaggc aaatcaccgt cgtcgtcagc gcagtcttaa
     9181 ttgaagacca ataagtcatc agatagatat tactgttttc actgtcgccg aaggggttaa
     9241 gggtatgcca aaaattttgg aaaatatcgg catagttttg atagctgacg gcgatgttca
     9301 ggcgacccaa gtcctcatct atcgtcgtta aaggagtaaa cggtggaatg gcgatttctt
     9361 gttcggcaga gctgattttc agcacgatgg cgaacggaat cagaaacagc accaaaagcc
     9421 aaatatacgg tacggcaatc accgcacgct gccccggacg gcggaacagt ttgtttttca
     9481 gtttattaag gttcatctca ctccccttaa atcaacggaa caacggagtc ggttggtttt
     9541 ccggccagct gatatagacg gtttcatccc aagtcggcgg cgtgatgtta cgcacatacc
     9601 agtaaggtgc aggaacttgg cttttgacga cgcgcccgtt ggcgagtttg atatggtata
     9661 tggcgaagct gcccaaatag gcaatttctt ttaccgtgcc tttcgcccag ttgtagtcgc
     9721 ccaaatattc gggtttttct ttatataaat caatatcctc tggtcgaata ctaacccaaa
     9781 gatcctgctc gctcgggcca cccaaaccat ggtcgatacg gacgtggttt tccaaacctt
     9841 cgcattcgat aacggcatag tcggcatgat tttcaatcac cacaccgtca aagatgttgg
     9901 tttcgccgat aaactcggca gtgaagcggc tgttgggata gtcgtacacg tcgctgggcg
     9961 tgccgacctg ctgcaactga ccgtcggaca taatggcgat gcgggtcgcc atcgtcatcg
    10021 cctcttcttg gtcgtgcgta accataatgc aggttacgcc gacttgttcc agcgtattga
    10081 ccaactcgag ctgggtttgc tggcgcaatt tcttatccaa cgcccccaaa ggttcgtcaa
    10141 gcaacaggat tttcggacgt tttgccaaac tgcgcgccaa ggcgatacgt tgctgctgac
    10201 caccggacaa ttggtgcggt ttgcgtttgg caaatttggt catctgaacc agacgcagca
    10261 tttcttcgac gcgcgcatcg atttcgcctt taggcatttt gtcctgtttc agaccgaagg
    10321 caatgttttg ctccaccgtc atatgcggaa aaagcgcgta actttggaac atcatattga
    10381 tggggcggtc atagggtgca agtttggtaa tatcttgacc atcaaggatg atttttccct
    10441 gattgggact ttccataccc gccagcatac gcagcagcgt agattttccg ctgccggaac
    10501 tgcccaaaag ggcgaagatt tcgttttgat aaatgttcaa gtcgatgtta tcgacagcgt
    10561 aattgtcacc aaactttttc accaaacctt ggatttgcag ataaggtttg gctgaagacg
    10621 ctgtggttgc ggtcataatg gcaatactcc aataaaaaga cgagtaccgg caaaacggat
    10681 tttcgaatgg gtgataaaaa gctgtttgat tgctggcggg agtcgcaacg tctgatgccg
    10741 tctgaaactc ttgtgaagcg cacgggcagc atgaaataaa aaggggttct aaagaacccc
    10801 gcattatatt agtttataag gtttttaggg taataaatat gggtttgagc ttgtcggaaa
    10861 gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg caattattta
    10921 cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg gcgcaaatag
    10981 gccaacaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct gtggtggtct
    11041 ttggggttgg ctttgaagtg gggggtcaga tcgttgatgc ggaaagtcaa cagggcaact
    11101 tgtacttcag aagagccggt gtcgccttct ttacgttgga aatctttaac gatttgtgct
    11161 ttttgttcta cggtcagtgc cataatggaa aaactccgaa aaataaaaag aaccccaaat
    11221 gggattcgga caagtttgcc aagcctcgac aacggcaaac tccctattcc tgaaacagga
    11281 acagcgcatt atcacacaat ttttgctgtt ctgcacgata ttttgcgatg tcggcgaaat
    11341 gccgtctgaa aacggagaca tcttccggtt tcagacggca tttgacatca gaccacaatt
    11401 tcttccttct gtttcgcttc cttggcgata ttgtcgcggc tcaaaccgaa catcagcaag
    11461 agcgggctgg caaccaatac ggaagagtag atgccgaaca cgataccgat ggtcagtgcc
    11521 atagaaaagc cgtgcaaggc tgcaccgccg aacaccagca tagaaacaac catcgcctcg
    11581 gtcgaaccgt gggtaatgat ggtgcggctc atcgttgcgg taatcgcgtt gtcaatgact
    11641 tccggcacgg tatgtccgcg catcgccggc ttgcggaaat tttcacggat acggtcgaag
    11701 acgacgacgg attcgttcac agaatagccc aatacggcaa ggatacctgc caagacggtc
    11761 agcgaaaatt cccattggaa gaaggcaaag cagccgagaa taatcacgat gtcgtgcata
    11821 ttggcgataa tggcagatac ggcaaaacgc cattcgaaac gcatcgacag gtaaataatg
    11881 atgccgataa cgacaaaacc taaagccatc aatccattat ttaccaattc ctcaccgact
    11941 tgcgggccga taaattcgac ttggcgcaaa gtaacgtcgg gactgtcttt tttcagcaaa
    12001 tccataacct gattggacaa ctgtgcggaa gtaacacctt ctttgttcgg caggcggatc
    12061 atgatgtgtt tgttcgtacc caatgcctga acctgtacat cacccatttt cagcgtatcg
    12121 aggcgttcgc gcatcttatt gacatccgca ccctgctgat attggacttc cattaccgta
    12181 ccgccggtaa attcgacaga gaaattcagg cctctggcaa ccaaaaagaa tacggcggca
    12241 ataaaggtaa ccaacgaaat gaaggttgtc agtttgccgt agctcataaa cggaatatcg
    12301 cgtttgattt taaagagttc catagcttac tccttacctg ccgcagtttc ggcttcaggt
    12361 ttccataccg caccgatgga aatattctgc aatttgcggc ggcggccgta ccacagattg
    12421 accaacgcac ggaatacgac gacggacgaa tacatcgaag tcagaatacc caagcagtgt
    12481 acgacggcaa aacctcttac cggtccggaa ccgaatacca aaagcgcgat accggcaatc
    12541 agcgaagtca ggttcgaatc aacaatggtc gcccatgcgt gttggaaacc gagattgatt
    12601 gcctgctgcg gcggcacgcc ggcacgcaat tcctctcgga tacgttcgtt aatcaagacg
    12661 ttggagtcga ttgccatacc caaagtcaac gccagcgcgg ccatacccgg taacgtcaac
    12721 gttgcctgca tggcagacaa aataccgatt aggaacagta tgttggcact caatgcaata
    12781 gtagaaaaga aacccatcag gcggtaataa accaccatga atgcagcaac gatggcaaaa
    12841 ccccataaag tcgaatggaa gcctttttcg atgttctcct tacccaaaga cggaccgatg
    12901 gtacgttctt cgacaatctg catcggtgcg gcaagagaac cggcacgcaa cagcaaagac
    12961 gtatcgttgg cttcggctgt cgtcatgctt ccggaaattt ccacgcgtcc gccggtaatg
    13021 gcagtacgga taaccggtgc ggttacaacc tcggattttc cttggtcgat caaaaccatc
    13081 gccatgcgtt tgccgacatt tgcggcagtc agttcgccga aaatgctgcc gcccgcgctg
    13141 tccaagctca gactgacggc aggtgcgccc atttggtcga aactcggttg cgcatcgttg
    13201 atgttgtcgc cggtcagctc gacctgtttg ctgatcagca gagtttcggg atgctccccg
    13261 ccgcttgaaa gcagctcata accgctcggc acgttgcctt ccaatgcctc gcgcaacttg
    13321 gcaggatcgt cctccaccat gcggacttcc aaagtcgcgg tacggccgat gatgtctttt
    13381 gccttggcag tatcttgcac gccgggaagc tgtacgacaa tgcggtccaa acctgactgt
    13441 tggataatcg gctctgccac acccaattcg tttacacggt tatgcagtgt agtgatgttt
    13501 tgcttcaccg catctgatcg tactttattc accgcctctt ccgaaagcgc caatacaata
    13561 ttgctgcctt ctgaatttaa cgttgcctca ggaaacagct tgagtaactg aggcagtgcc
    13621 ttttgcacat cgccggcatc ctgcaaaggg acggtcaggc tgttttcagc ctgacgcacc
    13681 gtgccgctgc ggattttttc acggcgcagt tcgcggcgga tgtcgcccga ataacgctca
    13741 aacgttttct gcatcgcggc tttcatatcg acctgcatgg tgaaatgcac gccgccgcgc
    13801 aggtccaaac ccaaaaacat cggattggct ttgattttcg ccatccattc ggggctgtcc
    13861 gccaacaggt tgagcgcggt aatataccct tcgcccaaag tgttttcgat aacgtcgcgc
    13921 gctttaagct gcgtttctgt gtctttgaaa cgcactttca gtgaattatc cacaacaaac
    13981 atcccgtcgg tctgaatgcc cgcgtttttc agcgcagcat ccactttgga ttgagtctgt
    14041 tcgttgatga tgatggcttg tcggttggtc gatacctgca cggcgggcgt ttcgccgaat
    14101 aggttgggca gcgaatacac tgcggcaacc gcaatcgtga acacaatcag cagatatttc
    14161 cataaaggat aacggttcat cattgttcct taatggttgg aaccccaccc tttcggtggt
    14221 gtcggaatcg ggctatttca gaagaggcaa aaacccctcc ctgccaggca agaccgaaag
    14281 gcagcatcct gaatatgccg ccctgcgtgt cggaacatgg tcaagccttc ggttggaatt
    14341 caaaacaaag tgccgcattc gggctttccc gatgcggctt gtcggcacaa atcaatcgac
    14401 ttttgcggca atcgcattgc gttccacttc gacctcgatt tttgtaccct gtccgatatc
    14461 cacggtaaaa aactgttcgc cgactctggt taccttaccc ttgaaacctg ccgccaagac
    14521 cactttgtcg ccggctttca aggcggcaag cattgcctga tgcgctttga atttcttttg
    14581 ttgggggcgc atgatcagga agtagaacac caccataatc aacaccaaag gagcaaattg
    14641 tgcaacagct tgattcataa tttatccgtt ctttctaaat atgattgaaa atcgagcggg
    14701 gtatataata gcataagacc gtaaacaata tatcgggttt gccgcccata cccaaccgta
    14761 tgccgtctgc accccctttc ccgaacccat gccgctgacc cgcctgttcc tgaaactcta
    14821 cgccctgttg cgcctttttt tgggcaaaaa cgcccagacc gcctggattt tccaccccgc
    14881 ctgcaccggg cacgaccccg gggcaaacca tcccgattcg cccgaccgca tcctctgcat
    14941 cgagcaggca ttgcgccgcg ccggtatttg gcagcacctc caaaccgtag aggcggaaga
    15001 aatcagcgat acgcgccttg cacttgtccg ctcgagcaaa tatctgaacc gtttggaatc
    15061 ttgcctaccc gaagacggca agatttgccg tttggacgac gacaccgtta tcagcaaaaa
    15121 ctccctgtcc gccgcccgtt tttccgccgg ttcggcagtt caggcagtcg atatggtcat
    15181 gaaacgcaaa gcgtggcacg ccttttgcgc cgcccgcccg ccggggcatc acgcaaaaag
    15241 cggcaaagcc ggcggattct gcctgctgaa caacgttgcc gccggcgtca tgcatgccat
    15301 tgccgaatac cgcctgaaac gcattgccgt catcgatttc gatgtccact acggcgacgg
    15361 tacggcagaa atattcaaag acgatccgcg catcctgttt ttcaacctgt ttgaaaccga
    15421 ccttttcccc ttccccgaaa acaacggtat gcccgacggc ggcaatatgg tgcacctgcc
    15481 cttgccgtcg ggaacgggca gccgcacatt ccgcgaagcc gtccgcagac agtggctgcc
    15541 ccggcttgcc gcattcaaac ccgaactggt gctgctgtcg gcaggattcg acgcacaccg
    15601 tctggacgaa tcgggcaggc tcaacctgca cgaggcggat tttgcctggc tgacacacaa
    15661 aatcattcag acggcatcca gctgccccgg caaaatcgta tccgtactcg aaggcggcta
    15721 cacccttgaa cctctggcac aatctgctgc cgagcatatc cgcgtactgg cggggctggg
    15781 caaatccgat gccgcaaccg cctatcagaa aacactgaac ccaacaaaaa aacggtttgc
    15841 caaaccgaaa accggacagg tgcgccaacc tacccaatcc gaccgttacg atataaatgc
    15901 cgtctgaagc cgattcggtt ttcagacggc atcattattt gacctgacgc aaacagaaag
    15961 ccttccgcgt gcttaaacac atctcttgag acctttgcaa aaatccccaa aatccc
//
LOCUS       DAWTCI010000048        15879 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWTCI010000048 DAWTCI010000000
VERSION     DAWTCI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15879
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.048"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(214..1494)
                     /gene="hemL"
                     /locus_tag="WM481_001424"
     CDS             complement(214..1494)
                     /gene="hemL"
                     /locus_tag="WM481_001424"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFA9080725.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIAIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGDEIACVIVEPFVGNMNLVRPTEA
                     FVKALRGLTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTALTQRLANG
                     IAAAKAHGIEFAADSVGGMFGLYFAAHVPRNYADMARSNIDAFKRFFHGMLDRGIAFG
                     PSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1558..1733)
                     /locus_tag="WM481_001425"
                     /pseudo
     CDS             complement(1558..1733)
                     /locus_tag="WM481_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            complement(1997..2071)
                     /locus_tag="WM481_001426"
                     /pseudo
     CDS             complement(1997..2071)
                     /locus_tag="WM481_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
     gene            2268..3596
                     /gene="miaB"
                     /locus_tag="WM481_001427"
     CDS             2268..3596
                     /gene="miaB"
                     /locus_tag="WM481_001427"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFA9080726.1"
                     /translation="MKKVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            3614..3778
                     /locus_tag="WM481_001428"
     CDS             3614..3778
                     /locus_tag="WM481_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080727.1"
                     /translation="MPPEAFQTAFCLVSADGTAAVIRTSGINFQTRNGTPSTARKPPP
                     EPFLPPAGFQ"
     gene            complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="WM481_001429"
     CDS             complement(3843..5756)
                     /gene="dxs"
                     /locus_tag="WM481_001429"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFA9080728.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAATDKLLGGDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKEGGAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVATALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDPKKLLDDLGLSAEAVER
                     RVREWLPDRDAAN"
     gene            complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="WM481_001430"
     CDS             complement(5841..6758)
                     /gene="xerC"
                     /locus_tag="WM481_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFA9080729.1"
                     /translation="MVLDGFAAYFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLDGRTLARKLSAWRQYCAWLVKRGLMRADP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLESWAAQYGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            6952..8016
                     /locus_tag="WM481_001431"
     CDS             6952..8016
                     /locus_tag="WM481_001431"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HFA9080730.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLGKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            8155..9012
                     /locus_tag="WM481_001432"
     CDS             8155..9012
                     /locus_tag="WM481_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HFA9080731.1"
                     /translation="MTRSKPVNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGL
                     ADALTAPLDHKDKGLKSLTLEASIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKND
                     KISRFDFVQKIEVDGQTITLASGEFQIYKQDHSAVVALRIEKINNPDKIDSLINQRSF
                     LVSDLGGEHTAFNQLPDGKAEYHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPE
                     QNVELASAELKADEKSHAVILGDTRYGGEEKGTYRLALFGGRAQEIAGSATVKIGEKV
                     HEIGIADKQ"
     gene            9151..9825
                     /gene="tsaB"
                     /locus_tag="WM481_001433"
     CDS             9151..9825
                     /gene="tsaB"
                     /locus_tag="WM481_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFA9080732.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGEIRLFHQEVGIRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIALPEGYVFSDG
                     IGSAFALENRPPFSGKPDMPTAADFLALALGGYPATGAAHAELLYVRNKIALTAKEQA
                     ERKART"
     gene            9822..10262
                     /gene="rimI"
                     /locus_tag="WM481_001434"
     CDS             9822..10262
                     /gene="rimI"
                     /locus_tag="WM481_001434"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFA9080733.1"
                     /translation="MNIRPALPSDCAALAALDTVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGRIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNAAAQALYTKHGFSIAGRRKNYYCAADGQTEDAVLMEKIC"
     gene            10256..10993
                     /locus_tag="WM481_001435"
     CDS             10256..10993
                     /locus_tag="WM481_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFA9080734.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAATIRPSQPHNVQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGILLDNILKAAGLDAAYVHKTCWVKTAAVGNPMP
                     SEQAVADALGQIAGELDGCRAPAVLFLGQAFVNLERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKRALRQGGGS"
     gene            11055..11696
                     /gene="pyrE"
                     /locus_tag="WM481_001436"
     CDS             11055..11696
                     /gene="pyrE"
                     /locus_tag="WM481_001436"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFA9080735.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGA
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DRGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRTYRRQYGVE"
     gene            11770..12198
                     /locus_tag="WM481_001437"
     CDS             11770..12198
                     /locus_tag="WM481_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080736.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAISKKQISRDEIADILNGGTTLHDTPPATAAAA
                     PAAAPQVSVPPARQEGLNWTIATLFALIVLIMQLSYLFIL"
     gene            12195..13505
                     /gene="argA"
                     /locus_tag="WM481_001438"
     CDS             12195..13505
                     /gene="argA"
                     /locus_tag="WM481_001438"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFA9080737.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLNKLA
                     ADIGLLSQLGIRLVLIHGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWMPPLGHSYGGKTFNLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAASETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNPHILVRRLHR"
     gene            13601..15616
                     /locus_tag="WM481_001439"
     CDS             13601..15616
                     /locus_tag="WM481_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HFA9080738.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGGAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADSKWQEAELPHLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRLQPQQFVSDGIEVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPKHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLACVLLFLKEFLG"
     gene            complement(15729..>15879)
                     /locus_tag="WM481_001440"
     CDS             complement(15729..>15879)
                     /locus_tag="WM481_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9080739.1"
                     /translation="LRLEHALALIESGSTIQAAMHFCGYRHAGRFNEAFRRHYGFLPS
                     DVKKC"
BASE COUNT         3579 a         4912 c         4243 g         3145 t
ORIGIN      
        1 cagacggcat ttttacagta gattgaaatc aaaaatgtac acgcccgtca ttcccgcgca
       61 ggcgggaatc cggaaggtcg ggcttgccgt tattttcaat cattacagaa actgaaaggt
      121 ccggattccc gcctgcgcgg aaatgacggc ggtgtgcatt cttacttcaa tctgccaaac
      181 aaatcgaaca gagaaactct gtccgtcaaa acatcatgca gccatcgcct tgaacacttc
      241 aaccgcaacc gcaaccgttt cgtcaatcag ctcgggcgta tgcgcggcgg aaacgaaacc
      301 tgcttcataa gcggacgggc cgaaggcaat gccgcggtcg agcatgccgt ggaagaagcg
      361 tttgaaagcg tcgatattgg agcgcgccat atcggcatag tttcgcggca cgtgtgcggc
      421 gaaatacaga ccgaacatac cgcccacgct gtcggcggca aactcgatac cgtgcgcttt
      481 ggcggcggca ataccgttgg caaggcgttg tgtcaaggcg gtcaggtttt catagaagcc
      541 ttcgcgctgg atgatttcca gcgttttcaa gccggcggcg acggcaatcg ggttgcctga
      601 taatgtacct gcctgataca cgccgcccaa cggggaaata cattccatga tgtctttgcg
      661 tccgccgaac gcggcaagcg gcataccgcc gccgatgact ttgcccatcg tggtcaggtc
      721 gggcgtgatg ccgtgcagcg actgcgcgcc gccgagcgcg acgcggaaac cggtcatcac
      781 ttcgtcgtaa atcaacaccg cgccgtgttt ttcggtcaat ccgcgcaagg ctttgacaaa
      841 ggcttcggtc gggcggacga ggttcatatt gccgacgaag ggttcgacaa tcacgcaggc
      901 gatttcgtcg ccgctttggg caaaggcttc ttcgagttgg gcgatgttgt tgtattcgag
      961 taccaaagta tgtttggtaa agtcggcagg cacaccggcg gaagaaggat tgccgaaggt
     1021 aagcagaccg ctgcctgctt tcaccaacag gctgtcggaa tggccgtggt agcagccttc
     1081 aaatttgatg attttgtcgc ggccggtaaa accgcgtgcc agacggatgg cagtcatcgt
     1141 cgcttccgtg ccggagctga cgaggcgcag ccgttcgaca gacggcataa tttcggcaat
     1201 ttgttcggca atggcgattt cgccttcggt gggcgcgccg aacgacaaac cgcccaacgc
     1261 agcttcgcgc acggcttcga cgacttcggg atgcgcgtgt ccgacaatcg caggccccca
     1321 agagccgaca taatcggtgt agcgcgtgcc gttttcgtcc caaacatacg cgccttcggc
     1381 ttttttgatg aagcgcggca cgccgccgac gctgccgaat gcgcgcacgg gcgaattcac
     1441 gccgccgggg atgatggctt tggcgcggtc gaataaaatt tcgttacggt tcatatttat
     1501 cctcaaatgc cgtctgaacg gcaggtttcg ggcttggaag cagaaagccc cattttatca
     1561 tttttcaggt tgcgacaagg atttgcccgc ttctttgcgg attacgccaa ccgtatcccg
     1621 gatgacggaa cgctcgtctt tttcctcttt atatgtgtaa agcggtagtc ccggacgact
     1681 ccctccccgt cgtaatccac gcaccattcc caatgccgcc gctccgattt catatagatg
     1741 aaattggtcg gcaaaaaatt ataaatcggc aggctgacct cgtgataggc ataacagccg
     1801 aaagggttgc gcttcccgaa acgtgcctct acctccgccc gggtcgtttt gcctttaaca
     1861 accgtttgtg cgattccctc ttccgtctga tatagtggat taacaaaaat caggacaagg
     1921 cgacgaagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg tactggttta
     1981 aatttaatcc actataacgc aggaactgat gttccctgtc gccgaaattg ctggtacacg
     2041 cacacagcag caatgccgcc catacacaca cctcccgtta aagccaaaca ttatacagcc
     2101 gttccgaccg attaaattca tattttaaaa caatattttg cctcccaaac ccacatcgtg
     2161 ctataatccg caccgatttt cagacggcat cgtcgtgccg tctgaaattt tttcattcca
     2221 acaaccatca gccccgcgat tacggctgcc cgagaaagac acaaaccatg aaaaaagtat
     2281 ttatccgcac cttcggctgc cagatgaacg agtacgacag cgaaaaaatg ctgtccgtcc
     2341 ttgccgaaga acacggcggc atcgaacagg ttacccaagc cgacgaagcc gacatcatct
     2401 tgttcaacac ctgttccgtg cgcgaaaaag cacaggaaaa agtgttctcc gacttggggc
     2461 gcgtccgtcc gctcaaagaa aaaaaccccg gcctcatcat cggcgttgcc ggctgcgtcg
     2521 cctcgcaaga aggcgaaaac atcatcaaac gcgcgcctta tgttgacgtg gttttcggcc
     2581 cgcaaacgct gcaccgcctg cccaaaatga ttgtggacaa agaaaccagc gggctgtcgc
     2641 aagtcgatat ttccttcccc gaaatcgaaa aattcgacca cctgccgccc gcccgcgtcg
     2701 aaggcggcgc ggcatttgtg tcgattatgg aaggctgttc caaatactgc tccttctgcg
     2761 tcgtccccta cacgcgcggc gaagaattct cccgcccgct caacgacgta ttgaccgaaa
     2821 tcgccaacct tgcccagcaa ggcgtgaaag aaatcaacct cttgggacaa aacgtcaatg
     2881 cctatcgcgg cgaaatggac gacggcgaaa tctgcgactt cgccaccctg ctgcgtatcg
     2941 tccacgaaat ccccggcatc gaacgtatgc gcttcaccac cagccacccg cgcgagttta
     3001 ccgactcgat tatcgagtgc taccgcgacc tgcccaaact ggtttcccac ctgcacctgc
     3061 cgattcaaag cggttccgac cgcgtattga gcgcgatgaa acgcggctac accgctttgg
     3121 aatacaaatc catcatccgc aaactgcgcg ccatccgtcc tgatttgtgc ctgagcagcg
     3181 acttcatcgt cggtttcccc ggcgagaccg aacgcgagtt cgagcaaacc ttgaaactgg
     3241 tgaaagacat tgccttcgac ttgagcttcg tgtttattta cagcccgcgc cccggcacgc
     3301 ctgccgccaa cctgccggac gacacgccgc acgaagaaaa agtgcgccgc ctcgaagcct
     3361 tgaacgaagt catcgaagcc gaaaccgccc gcatcaacca aaccatgatc ggcacggttc
     3421 aacgctgcct ggtcgaaggc atctccaaaa aagaccccga ccagctccaa gcccgtaccg
     3481 ccaacaaccg cgtcgtcaac tttaccggca cgcccgacat gatcaaccaa atgatcgatt
     3541 tggaaatcac cgaagcctac accttttccc tgcgcggcaa aattgtcgaa gcctaaaacc
     3601 ctcacgccga aaaatgccgc ctgaagcgtt tcagacggca ttttgccttg tatcggcaga
     3661 cggcaccgcc gccgttatac ggacaagcgg aataaatttc caaacgcgca acggcacacc
     3721 aagcaccgcc cgaaagccgc cgcccgaacc cttcctgccg cctgcggggt ttcaataaaa
     3781 aaaccgcaga aacaaacgcc gtttaaaaac gccgcccgat aacgacggcg cggtcaagcg
     3841 gtttaatttg ccgcatcacg gtccggcagc cactcgcgca cccggcgttc caccgcttcg
     3901 gcactcaaac ccaaatcgtc caaaagtttt ttcggatcgc cgtgttcggt tacggtatcg
     3961 gcaacgccca aaagcaaaac gggtttgcag atgccgtgtt tcgccaacac ttccaagacc
     4021 gcgccgcctg cgccgccctg ttcggcgttt tcttcaaggg taacgatgcg gtcgtggctt
     4081 cgggcaaggc ggacaatcaa ctcttcgtct atcggtttga cgaagcgcat atcggcgacg
     4141 gtggcgttca gtttttcggc aaccgccaat gcggtggcga ccatactgcc gaaggcaatg
     4201 aaggcggttt tctcaccttc gcggcggata atgcccttgc cgatttccac ggtttccatg
     4261 ccgtctgaaa ccggcgcgcc cgtacccgtg ccgcgcggat agcggacggc ggcgggcgca
     4321 tccgcctgat agcaggtcga aagcagcagg cggcattcgt tttcatcgct cggcgcggca
     4381 acaatcatgt tcggcacaca gcgcaagaag ctcaaatcgt acaagccggc atgggtcggg
     4441 ccgtccgcgc cgacgatgcc cgcacggtcg acggcaaaca aaacgggcag gttttgcagg
     4501 gcgatgtcgt gcaccagttg gtcgtaggcg cgttgtaaaa aggtggaata aatcgccacg
     4561 acgggcttca tgccttcgca cgccaaaccg ccggcaaagg taacggcgtg ctgctcggcg
     4621 atgccgacat cgaaatagcg gtcggggaat cgttgttcaa actccaccag tccgctgccc
     4681 tcgcgcatgg cgggggtaat cgcaaccagt cgggaatctg ccgccgcccg gtcgcacagc
     4741 catttgccga atacttgggt ataggtcggt ttggcggcgg gcttgggttc tttttcagac
     4801 ggcatttgcg ccccgccttc tttaggcagg ttggcgacgg cgtggtattt gacggggtcg
     4861 ttttcggcga gtttgtagcc gttgcccttt ttggtgatga cgtgcagcaa ctgagggcct
     4921 ttgcggctgc gcaagtcttt caatacgtcc accagattct cgacgttgtg tccgtccacg
     4981 gggccggtgt agcggaagcc gaaattttca aacagcgaca gcgactgttt ggcgtgttcg
     5041 gcttcttcgg caagggtttt gattttgtgt tcgacttttt gggcaaactc catcgcgccg
     5101 ggtattttgt ctaatacctt gcccgtttgc gctttgacgg tactcaacag tccgtgcata
     5161 tcgcgcacga cgttgctggc aagatatttg ggcaacgcgc cgacgttggg ggaaatcgac
     5221 atttcgttgt cgttgaggac gaccagcaaa tccacatcca tatcgcccgc gcaattcaag
     5281 gcttcaaacg cctgccccgc cgtcatcgcg ccgtctccga tgatggcgac gctgcggcgg
     5341 tcgccgccca agagtttgtc cgtcgccgcc atgcccaaag ccgcgccgat ggaggtggag
     5401 gaatgcccca cgccgaacgc gtcgtactcg gactcgcaac gtttcggaaa acccgccaaa
     5461 ccgccgtatt ggcgcatggt gtgcatctgg tttttcctgc ctgtcaggat tttgtgcggg
     5521 tagctttggt gtccgacatc ccacaccagc ttgtcttcgg gcgtgtcgta cacatagtgc
     5581 agggcgatgg tcagttcgac cgcacccaga ttgctggcga aatgcccgcc ggtctgcccg
     5641 acagattcca gcagaaaggc gcgcaactcg ccggcaaggc gcggcagctg ttttttgtcc
     5701 aggcggcgca aatcttgcgg gctgtcaatc aggtcgagta gggggcttgg gttcatggtg
     5761 tgtctttttt atgtgtcgtc cgggtgcaac ggtcaattat atatcaagag cgtgcggctg
     5821 acggctgatt ttgccgtatg tcattcgtcc tgccgcttgg cgcgcgggtg ggcttcgtca
     5881 tagaggcggg cgatgtggtc gaaatcgagc ttggtataaa tctgcgtggt cgaaaggctg
     5941 ctgtgtccga gcagctcctg caccgccctg atgtcgcgcg aagcctgcaa caggtggccg
     6001 gcgtagctgt ggcgcatcat atgcggcgaa acgtgcctgc cgtcgccgta ttgcgccgcc
     6061 cacgattcga ggcgtttttg gatttggcgt tggctcaggc gcgtgccgtt cctgccggta
     6121 aacagggctt tgccgtccga tgccgtctga cgcagcggca gatagttttt caaggcttcc
     6181 acgcttttgc cggtcagcgg aacctgacgc tgcttgcgcc ctttgccggt aacgtgtacc
     6241 cacgcttcgt ccaaatatac atcatctgca ttcaagccgt gtatctcgct cacgcgcaaa
     6301 ccgctgccgt acatcagctc gaacagcgcg tggtcgcgca ccgccagcgg gtcgccgccg
     6361 tccacgggca aatccaacat ccggttcagc cattcctgcg gcagggcttt gggtacgcgc
     6421 tcgggctgct tcggcggttt gatgtcggcg gtcgggtcgg cgcgcatcag gccgcgtttg
     6481 accagccagg cgcaatactg ccgccacgcc gacagcttgc gcgccagcgt ccgaccgtcc
     6541 aaaccgcgct gcgacagccg ccgcaacgcc gccgtaaaat cgcgccgcga caagtcctgc
     6601 ggcacgccgc ctgcatcttc agacggcatt tgtgccaaca gtgcaaacag ttcttccaaa
     6661 tcgcgccggt atgccgcaac cgtgtgctcc gacttgccct cgcgcacgat gttttccaaa
     6721 taagcgtcaa aatacgccgc aaacccgtcc aaaaccataa ccgtcccaca caaatatcaa
     6781 aaaaccagtg aaaacattat cataaacaac acaccaaaat cccgaaccgc ccaaaatccg
     6841 ccccatctgc ccgccgttat cgttaatata aaatcaatga gccgtttatg gttttttgcc
     6901 gtaaaaaaca ttataatccg ccttatttac ctattgccca aggagacaca aatggcactc
     6961 gtatccatgc gccaactgct tgaccacgcc gccgaaaaca gctacggcct gcccgcgttc
     7021 aacgtcaaca acctcgaaca aatgcgcgcc attatggaag ccgccgacca agtcaacgcg
     7081 cccgtcatcg tacaggcgag cgcaggtgcg cgcaaatacg cgggcgcgcc gtttttgcgc
     7141 cacctgattc tggcggcagt cgaagaattt ccgcacatcc ccgtcgtgat gcaccaagac
     7201 cacggcgcat cgcccgacgt gtgccaacgc tccatccaac tgggcttctc ctccgtgatg
     7261 atggacggct ctttgctcga agacggcaaa accccttctt cttacgaata caacgtcaac
     7321 gccacccgta ccgtcgtcaa cttctcccac gcctgcggcg tgtccgtcga aggcgaaatc
     7381 ggcgtattgg gcaacctcga aaccggcgaa gcaggcgaag aagacggagt gggcgcggca
     7441 ggcaaactct cacacgacca aatgctcacc agcgttgaag atgccgtgcg tttcgttaaa
     7501 gataccggcg ttgacgcatt ggcgattgcc gtcggcacca gccacggcgc atacaaattc
     7561 acccgtccgc ccacaggcga cgtattgcgt atcgaccgca tcaaggaaat ccaccaagcc
     7621 ctgcccaata cacacatcgt gatgcacggc tccagctccg ttccgcaaga atggctgaaa
     7681 gtcatcaacg aatacggcgg caatatcggc gaaacctacg gcgtgccggt tgaagaaatc
     7741 gtcgaaggca tcaaacacgg cgtgcgcaaa gtcaacatcg ataccgacct gcgcctcgct
     7801 tccaccggcg cggtacgccg ctaccttgcc gaaaacccgt ccgactttga tccgcgcaaa
     7861 tacttgggca aaaccattga agcgatgaag caaatctgcc tcgaccgtta tcttgcgttc
     7921 ggttgcgaag gtcaggcagg caaaatcaaa cctgtttcgt tggaaaaaat ggcaagccgt
     7981 tatgccaagg gcgaattgaa ccaaatcgtc aaataacagg ttgcctgtaa acaaaatgcc
     8041 gtctgaacgc cgttcggacg gcatttgatt tttgcttctc tgacctgcct cattgatgca
     8101 gtatgcaaaa aaagatacca taaccaaaac gtttatatat tatctattct gtgtatgact
     8161 aggagtaaac ctgtgaaccg aactaccttc tgctgccttt ctttgaccgc cggccctgat
     8221 tctgaccgcc tgcagcagcg gaggggcgga ggcggtggtg tcgccgccga catcggcacg
     8281 gggcttgccg atgcattaac cgcgccgctc gaccataaag acaaaggttt gaaatcccta
     8341 acattggaag cctccattcc ccaaaacgga acactgaccc tgtcggcaca aggtgcggaa
     8401 aaaactttca aagccggcgg caaagacaac agcctcaaca cgggcaaact gaagaacgac
     8461 aaaatcagcc gcttcgactt cgtgcaaaaa atcgaagtgg acggacaaac catcacactg
     8521 gcaagcggcg aatttcaaat atacaaacag gatcactccg ccgtcgttgc cctacggatt
     8581 gaaaaaatca acaaccccga caaaatcgac agcctgataa accaacgctc cttccttgtc
     8641 agcgatttgg gcggagaaca taccgccttc aaccaactgc ctgacggcaa agccgagtat
     8701 cacggcaaag cattcagctc cgacgatgcc ggcggaaaac tgacctatac catagatttc
     8761 gccgccaaac agggacacgg caaaatcgaa cacctgaaaa cacccgagca gaatgttgag
     8821 cttgcctccg ccgaactcaa agcagatgaa aaatcacacg ccgtcatttt gggcgacacg
     8881 cgctacggcg gcgaagagaa aggcacttac cgcctcgccc ttttcggcgg ccgcgcccaa
     8941 gaaatcgctg gctcggcaac cgtgaagata ggggaaaagg ttcacgaaat cggcatcgcc
     9001 gacaaacagt agtatttgaa aaatgccgtc cgaacacgat aatttaccgt tcggacggca
     9061 tttttgcgcc ataccgccct aggacacgac gacacggcag gcaagccgat ttcactatat
     9121 aatgccgtct gaaccaacga gaaccacacc atgcaagccg atttcaaccg ccccgtcctc
     9181 gccgtcgata ccggaacttc ctatttgtcg ctcgcgctgc gcgccgacgg cgaaatccgt
     9241 ctgttccatc aggaagtcgg catccgccag tccgaactga tcctgccgga aatccgcacc
     9301 ctattccgaa atgcgggcat taccgccgcc gatttggggg cgattgttta tgcgaaaggc
     9361 cccggcgcgt ttaccggact gcgtatcggc atcggcgtgg cgcaaggttt ggcaacgccg
     9421 ttcgacacgc ctttaatcgg catccccacg ctcgatgccg ccgcctcgct gccgccgccg
     9481 caaagctgca tactcgccgc tgcggacgcg cgtatgggcg aagtgtttta tgcgtggttc
     9541 gacacgctga accgccgccg tttgagcgat tatcaagtcg gtagggcggc agacattgcc
     9601 ctccccgagg ggtacgtctt ttcagacggc atcggcagtg cgttcgcact ggaaaaccgt
     9661 ccgcccttct caggcaaacc cgatatgccg accgccgccg attttctcgc gctcgccctc
     9721 ggcggctatc ccgcaactgg cgcggcacac gccgaactgc tctacgtccg caacaaaatc
     9781 gccctgactg ccaaagaaca ggcggaacgg aaggcacgaa catgaacatc cgcccagcct
     9841 taccgtccga ctgcgccgca ctggccgcgc tcgataccgt ctgcaacccg tccgcgtgga
     9901 cgcaacgcca atttgagtcc gcgcttgttt cgccgtccga acaggttttc cttgcggaaa
     9961 aagacggcag gattgccgcc tttatcgttt ggcagaacct gcccgacgaa tccgaactgc
    10021 acctgattgc caccgcgccc gaatgccgcc gacggggcgt tgcgtccgcc ctgctcgaat
    10081 attggtacgc acatctgccc gaaggcacgc aacgcctgct gctcgaagtc cgcgcaggca
    10141 acgccgccgc acaggcactg tataccaaac acggcttcag cattgcgggc aggcggaaaa
    10201 actattactg cgccgccgac gggcaaaccg aagatgccgt cttaatggag aaaatatgtt
    10261 aagcgcgcgc tacctccacc tgcacgaggc tttgggcttg ggtccgatgt ggctgaaaca
    10321 ggccgccgcc gtcctgccgc ccaaaaacac acccgcaacc ccggcacagg cacgtcccca
    10381 aaccgtccgc gccgccacga tccgcccttc ccaaccccat aacgttcagg cgcggctcga
    10441 aacgatgaaa gcgttggaaa ccgccgccgt acatacgcgc aaacccgcgc ctgaaaccga
    10501 aacgcccccg cccggccttt cagacggcat cgcccccgtt cccgccgctt cgggcattac
    10561 caagcttgcc gtcgtcagcc tgtgtccgcc gatcgaggat gcggtttacg ggcaactgtt
    10621 ccacggcaaa gcgggcatcc tgctcgacaa catcctcaaa gccgcaggac tggatgccgc
    10681 ctatgtccac aaaacctgtt gggtgaaaac cgccgccgtc ggtaacccga tgccgtctga
    10741 acaggccgtc gcggatgcgc tgggtcaaat cgccggggaa ctcgacggct gccgcgcccc
    10801 cgccgtccta ttcctcggac aggcgtttgt caacctggaa cggcaggcga tgattgaaac
    10861 tttgtgcggc agccgtccct tcttcatcat cgaccatccc gcccggctgt tgcgccaacc
    10921 cgaactcaaa gcccgaaact ggcaggtgtt gaaacagttg aaacgcgcct tgcggcaagg
    10981 cggcggcagt tgaagcgcgc cgcacggggc ggtagaatcg caactgcgtc ccaatatctg
    11041 acagaaagca caaaatgact gatttccgcc aagatttcct caaattctcc ctcgcccaaa
    11101 atgttttgaa attcggcgaa tttaccacca aagccggacg gcggtcgccc tatttcttca
    11161 atgccggcct cttcaacgac ggcgcgtcca cgctgcaact ggcaaaattc tatgcacaat
    11221 ccatcattga aagcggcatc cgattcgata tgctgttcgg ccccgcctac aaaggcatta
    11281 ttttggcggc ggcaaccgcg atgatgctgg cggaaaaagg cgtgaacgtc ccgtttgcct
    11341 acaaccgcaa agaagccaaa gaccgcggcg aaggcggcgt gttggtcggc gcgccgctta
    11401 aagggcgcgt gctgattatc gacgacgtga tttccgccgg cacatccgta cgcgaatcaa
    11461 tcaaactgat tgaagcggag ggtgcaaccc ccgccggtgt cgccatcgcg ctcgaccgca
    11521 tggaaaaagg cacgggtgaa ttgtccgccg ttcaggaagt ggaaaaacaa tacggcctgc
    11581 ccgtcgcccc catcgccagc ctgaacgatt tgtttatcct gttgcaaaac aaccccgaat
    11641 tcggacagtt cctcgaaccc gtccgcacct accgccggca gtacggcgta gaataaaaat
    11701 aaagcatatg ccgtccgaac cacctgccgc ttcagacggc atcaaaccga cacacaccga
    11761 gaaaacatca tgcccgcctg tttctgtccg cactgcaaaa cccgcctctg ggtcaaagaa
    11821 acccagctca acgtcgccca aggcttcgtc gtctgccaaa aatgcgaagg gctgtttaaa
    11881 gccaaagacc atctggcaag cacgaaagaa cctatattca acgatttgcc cgaagctgtt
    11941 tcggatgtca aactcgtcca ccgcatcggc acgcacgcca ttagcaagaa acagatttcc
    12001 cgcgacgaaa tcgccgatat cctcaacggc ggtacaaccc tgcacgatac gccgcccgca
    12061 accgccgctg ccgcacctgc cgccgcaccg caggtttccg taccgcccgc ccgtcaggaa
    12121 gggctcaact ggactattgc aaccctgttc gcacttatcg tcctcattat gcagctttcc
    12181 tacctcttca tcctatgaac gcgcccgaca gctttgtcgc ccacttccgc gaagccgccc
    12241 cctacatccg ccaaatgcgc ggcacgacac tggtcgccgg catagacggc cgcctgctcg
    12301 aaggcggcac cttaaataag ctcgccgccg acatcgggct gttgtcgcaa ctgggcatcc
    12361 gactcgtcct catccacggc gcgtaccact tcctcgaccg cctcgccgcc gcgcaaggcc
    12421 gcacgccgca ttattgccgg ggtttgcgcg ttaccgacga aacctcgctc ggacaggcgc
    12481 agcagtttgc cggcaccgtc cgcagccgtt ttgaagccgc attgtgcggc agcgtttcag
    12541 gattcgcgcg cgcgccttcc gtcccgctcg tatcgggcaa cttcctgacc gcccgtccga
    12601 taggcgtgat tgacggaacc gatatggaat acgcgggcgt tatccgcaaa accgacaccg
    12661 ccgccctccg tttccaactc gacgcgggca atatcgtctg gatgccgccg ctcgggcatt
    12721 cctacggcgg caaaaccttc aatctcgata tggtgcaggc cgccgcttcc gtcgccgtct
    12781 cgcttcaggc cgaaaaactc gtttacctga ccctttcaga cggcatttcc cgccccgacg
    12841 gcacgctcgc cgaaaccctc tcggcacagg aagcgcaatc gctggcggaa cacgccgcca
    12901 gcgaaacccg acgactgatt tcgtccgccg ttgccgcgct cgaaggcggc gtgcatcgcg
    12961 tccaaatcct caacggggcc gccgacggca gcctgctgca agaactcttc acccgcaacg
    13021 gcatcggcac gtccattgcc aaagaagcct tcgtctccat ccggcaggcg cacagcggcg
    13081 acatcccgca catcgccgcc ctcatccgcc cgctggaaga acagggcatc ctattgcacc
    13141 gcagccgcga atacctcgaa aaccacattt ccgaattttc catcctcgaa cacgacggca
    13201 acctgtacgg ctgtgccgca ctcaaaacct ttgccgaagc cgattgcggc gaaatcgcct
    13261 gccttgccgt ctcgccgcag gcacaggacg gcggctacgg cgaacgcctg cttgcccaca
    13321 ttatcgataa ggcgcgcggc ataggcataa gcaggctgtt cgcactgtcc acaaataccg
    13381 gcgaatggtt tgccgaacgc ggctttcaga cggcatcgga agacgagctg cccgaaacgc
    13441 ggcgcaaaga ctaccgcagc aacggacgaa acccgcatat tctggtgcgt cgcctgcacc
    13501 gctgaccgca acggaaagcc gccgcagaaa tgccgcctga accccgtttc agacggcatt
    13561 tccccgatga caaagacctg cccgaccctc aaggaccccg atgaaatcct accccgaccc
    13621 ctaccgccat tttgaaaacc tcgattccgc cgaaacgcaa aacttcgctg ctgaagcgaa
    13681 tgccgaaacg cgcgcgcgtt ttttagaaaa cgacaaggcg cgcgcacttt cagacggcat
    13741 tttgaatcaa atgcaggaca cgcggcagat tccgttttgt caggaacacc gcgcgcggat
    13801 gtaccatttc catcagaatg cggaatatcc gaagggcgtg taccgcatgt gtacggcggc
    13861 gacctaccgt tccggctatc ccgagtggaa aatcctgttt tcggtggcgg atttcgatga
    13921 gttgctcggc gacgatgtgt atttgggcgg cgtgtcgcac ttggtggagc agcccaaccg
    13981 cgcgctgctg actttgaaca aatcgggcgg cgatacggcg tatacgctgg aagtggattt
    14041 ggaagcaggg gaattggtag agggcggttt tcactttccg gcaggcaaaa accatgtgtc
    14101 gtggcgcgat gaaaacagcg tgtgggtgtg tccggcttgg gacgaacgcc agttgaccga
    14161 atcgggctat ccgcgcgaag tgtggctggt ggaacgcggc aagagtttcg aggaaagcct
    14221 gccggcgtac caaatcgata aaggcgcgat gatggtaaac gcgtggcgtt acctcgatcc
    14281 gcagggttcg ccgattgatt tgattgaagc gtcggacggt ttttacacca agacgtattt
    14341 gcaggtgtcg tccgaaggcg gggcgaaacc gttgaacctg cctaatgatt gcgatgtggt
    14401 cggctatctg gcgggacatc ttttgctgac gctgcgcaag gactggcacc gcgcgaacca
    14461 aagctatccg agtggcgcgt tggtggcggt gaaactgaat cggggcgaac tcggggcggc
    14521 gcagcttttg tttgcgcccg atgaaacgca ggcattggaa agcgtggaaa cgaccaagcg
    14581 ttttgtggtg gcaagcctgc tggagaatgt acaaggccgt ctgaaagcgt ggcggtttgc
    14641 cgacagcaaa tggcaggaag ccgagttgcc gcacctgccc tcgggcgcgt tggaaatgac
    14701 cgaccaaccg tggggcggcg acgtggttta tcttgccgcc agcgatttca ccacgccgct
    14761 gacgctgttt gcgctggatt tgaacgtgat ggaactgacc gtcatgcgcc tccagccgca
    14821 gcagtttgtt tcagacggca tcgaagtgcg gcagttttgg gcggtgtcgt ccgacggcga
    14881 acgcattcct tatttccacg tcggcaaaaa cgccgcgccc gacacgccga ccttagtcta
    14941 tgcttacgga ggtttcggca ttcctgaatt gccgcattat ctgggcagcg tcggcaaata
    15001 ttggctggaa gagggcaatg cctttgtatt ggcaaacatc cgcggcggcg gagaattcgg
    15061 cccgcgctgg catcaggcgg cgcagggaat cagcaaacac aaaagcgttg atgatttgtt
    15121 ggcagtcgtg cgtgatttgt ccgaacgcgg catgagttcg cccaaacaca tcggcttgca
    15181 gggcggcagc aacggcggcc tgattaccgc cgccgccttc gtgcgcgaac cgcaaagcat
    15241 cggtgcgctg gtgtgcgaag taccgctgac cgatatgatc cgttatccgc tgctgtccgc
    15301 cggttcaagt tggacggacg aatacggcaa tccgcagaaa tacgaagcct gcaaacgccg
    15361 gctgggcgaa ttgtcgccgt atcacaatct ttcagacggc atcgattatc cgcccgcact
    15421 cattaccacc agcctcagcg acgaccgcgt ccatcccgcc cacgcgctca aattctacgc
    15481 caaactgcgc gaaacctcgc cgcaatcttg gctctactcg cctgacggcg gcggccatac
    15541 cggcaacggc acccaacgcg aatccgccga caaactcgcc tgcgtgttgc tgtttttgaa
    15601 agaatttttg ggataagggc agggggcgac actgccgccg cgaatgaaaa aagatcgtct
    15661 gaaattgctt tttcagacga tcttttttaa tggttgtttc aaatcaaaat atctatgccg
    15721 tcggcccatc agcacttttt cacatccgaa ggcaaaaatc cgtaatgccg cctgaacgct
    15781 tcgttgaacc gtcccgcgtg gcggtagccg caaaagtgca tcgcggcttg gatggtgctg
    15841 cccgattcga tgagggcgag cgcgtgttcc agccgcagg
//
LOCUS       DAWTCI010000049        15805 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWTCI010000049 DAWTCI010000000
VERSION     DAWTCI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15805
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.049"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            195..1538
                     /locus_tag="WM481_001441"
     CDS             195..1538
                     /locus_tag="WM481_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFA9080740.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYVGMRPSMK
                     IKEGEAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYVPEALAKLSSEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFVTGRLNTERVVALGGLQVNKPRLL
                     RTVLGAKVSQLTAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRGKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1541..2773
                     /locus_tag="WM481_001442"
     CDS             1541..2773
                     /locus_tag="WM481_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFA9080741.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYVLYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSPEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVANPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2766..3542
                     /locus_tag="WM481_001443"
     CDS             2766..3542
                     /locus_tag="WM481_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFA9080742.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIRPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGVQ"
     gene            3542..4168
                     /locus_tag="WM481_001444"
     CDS             3542..4168
                     /locus_tag="WM481_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFA9080743.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4172..4765
                     /gene="nqrE"
                     /locus_tag="WM481_001445"
     CDS             4172..4765
                     /gene="nqrE"
                     /locus_tag="WM481_001445"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFA9080744.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYDFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4779..5996
                     /gene="nqrF"
                     /locus_tag="WM481_001446"
     CDS             4779..5996
                     /gene="nqrF"
                     /locus_tag="WM481_001446"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFA9080745.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNDEKEL
                     TMPAGGKLLGALASQGIFVPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLHSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6513..7568
                     /locus_tag="WM481_001447"
     CDS             6513..7568
                     /locus_tag="WM481_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFA9080746.1"
                     /translation="MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILQQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNAHGEPWRIGIEQPNIIQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVSDSAMTADGLSTGLFVLGETEALRLAEQEKLAVFLIVRD
                     KDGYRTAMSSEFAKLLR"
     gene            7594..7809
                     /gene="nqrM"
                     /locus_tag="WM481_001448"
     CDS             7594..7809
                     /gene="nqrM"
                     /locus_tag="WM481_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFA9080747.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="WM481_001449"
     CDS             complement(7933..8511)
                     /gene="grpE"
                     /locus_tag="WM481_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFA9080748.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8694..9512
                     /gene="cysE"
                     /locus_tag="WM481_001450"
     CDS             8694..9512
                     /gene="cysE"
                     /locus_tag="WM481_001450"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFA9080749.1"
                     /translation="MKKNHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQISKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARLGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFAEIDFMI"
     gene            complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="WM481_001451"
     CDS             complement(9716..11227)
                     /gene="ubiB"
                     /locus_tag="WM481_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFA9080750.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCVSLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGKRLKPREVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HVESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKINALVDEARRQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(11262..11471)
                     /locus_tag="WM481_001452"
     CDS             complement(11262..11471)
                     /locus_tag="WM481_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HFA9080751.1"
                     /translation="MELETVVPPEEGEGEGGGADGEMPEYLELHGGREDDISASSQPE
                     PPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            complement(11716..11862)
                     /locus_tag="WM481_001453"
     CDS             complement(11716..11862)
                     /locus_tag="WM481_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080752.1"
                     /translation="MPSERSDGIFTFGKYIIGMNLHIIRLLEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="WM481_001454"
     CDS             complement(11879..12217)
                     /gene="erpA"
                     /locus_tag="WM481_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFA9080753.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(12350..13036)
                     /locus_tag="WM481_001455"
     CDS             complement(12350..13036)
                     /locus_tag="WM481_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9080754.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVGHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTV"
     gene            13223..13495
                     /locus_tag="WM481_001456"
     CDS             13223..13495
                     /locus_tag="WM481_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080755.1"
                     /translation="MKKTSKYLIYTAAFTSFCFAFQENRSEAKQPDITLSASLCEQFN
                     MLNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGVVYE"
     gene            13703..15631
                     /gene="dnaK"
                     /locus_tag="WM481_001457"
     CDS             13703..15631
                     /gene="dnaK"
                     /locus_tag="WM481_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFA9080756.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSIIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKADNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKIAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGRFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGEGAQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         4109 a         4358 c         3716 g         3622 t
ORIGIN      
        1 caaatcaata tcccccttcc gacaattaaa aacggcttcc gcaaggaaaa aagcggcaaa
       61 tctgacaaac aggtacaatt cctgataaaa tctaacactt tgctacatta atttattcaa
      121 tgcttttgat gccacgcgac acggtatcaa aaacacgccg tttttacctt aaccatcaca
      181 agtaatgcaa atctatgatt aaaatcaaaa aaggtctaaa tctgcccatc gcgggcagac
      241 cggagcaagt catttatgac ggcccggcca ttaccgaagt cgcgttgctt ggcgaagaat
      301 atgtcggcat gcgcccctcg atgaaaatca aggaaggtga agccgtcaaa aaaggccaag
      361 tgctgtttga agacaaaaag aatccgggcg tagtatttac tgcgccggct tcaggcaaaa
      421 tcgccgctat tcaccgtggc gaaaagcgcg tacttcagtc agtcgtgatt gccgttgaag
      481 gcaacgacga aatcgagttc gaacgctacg tacctgaagc gctggcaaaa ttgagcagcg
      541 aagaagtgcg ccgcaacctg attcaatcag gcttatggac tgcgcttcgc acccgtccgt
      601 tcagcaaaat ccctgccgta gatgccgagc cgttcgccat cttcgtcaat gcgatggaca
      661 ccaatccgct ggctgccgac cctacggtca tcatcaaaga agccgccgaa gacttcaaac
      721 gcggcctgtt ggtattgagc cgcctgaccg aacgtaaaat ccatgtgtgt aaagcagcag
      781 gcgcagacgt gccgtctgaa aatgctgcca atatcgaaac acatgaattt ggcggcccgc
      841 atcctgccgg cttgagtggc acgcacattc atttcatcga gccagtcggc gcgaataaaa
      901 ccgtgtggac catcaattat caagacgtga ttgctatcgg acgtttgttc gtaacaggcc
      961 gtctgaatac cgagcgcgtg gttgccttgg gcggcctgca agtcaacaaa ccgcgcctct
     1021 tgcgtaccgt tttgggtgcg aaggtgtctc aacttaccgc cggcgaattg gttgacgcgg
     1081 acaaccgcgt gatttccggt tcggtattga acggtgcgat tgcacaaggc gcgcatgatt
     1141 atttgggacg ctaccacaat cagatttccg ttatcgaaga aggccgcggc aaagagctgt
     1201 tcggctgggt tgcgccgcag ccggacaaat actccatcac gcgcaccact ctcggccatt
     1261 tcctaaaaaa caaactcttc aagttcacga cagccgtcaa cggcggcgac cgcgccatgg
     1321 taccgatcgg cacttatgag cgcgtaatgc cgttggacat cctgcctacc ttgcttttgc
     1381 gcgatttaat cgtcggcgat accgacagcg cgcaggcttt gggttgcttg gaattggacg
     1441 aagaagacct cgctttgtgc agcttcgtct gcccgggcaa atacgaatac ggcccgctgt
     1501 tgcgcaaagt gctggaaacc attgagaagg aaggctgatt atgggcttaa aacattttct
     1561 ggaaaaaatc gaaccgcact tcctgccggg cggcaaacat gaaaaatggt atgtcctcta
     1621 tgaagctgcg gcgacgattt tctatacatc cggcgcggta acgcgcaaag cggcgcacgt
     1681 ccgcgacgcg ctcgactcca aacgcatgat gattttggtg tggctggctt tgttccccgc
     1741 catgttttac ggcatgtaca acgtcggcgc acaggcattc ggtgccttaa cgcccgattt
     1801 gctgcaacaa agcatcgcca acgactggca ttacgccctt gccaacgctt tgggcatcaa
     1861 tatgtcgcct gaagcgggcg tgttgggcaa aatgctgttc ggcgcgattt acttcctgcc
     1921 gatttacgcg accgtattta ttgtgggcgg cttctgggaa gtcttgttcg catccgtacg
     1981 caaacacgaa atcaacgaag gtttcttcgt tacttcgatt ctgtttgcct taatcgttcc
     2041 gcccacgctg ccgctgtggc aggcggcttt gggtatttct ttcggcgttg tggttgcgaa
     2101 agaagtattc ggcggtacag gtaaaaactt catgaaccct gcgctggcag gccgcgcctt
     2161 cctgttcttc gcctaccccg ccaacttgag cggcgatgcg gtttggacgg cggttgacgg
     2221 ctattccggc gcaaccgcgc tggcgcaatg ggcggcacac ggtgcagacg gcctgaaaaa
     2281 cgccgtaacc ggtcaaacca tcacttggat ggacgcgttt atcggcaaac tgcccggctc
     2341 catcggcgaa gtctccactt tggcactctt aatcggcggc gcgtttatcg tgtttgcccg
     2401 catcgcttct tggcgcatta ttgccggcgt gatgatcggt atgattgcga tgtcttcgct
     2461 gtttaacttc atcggttctg acaccaacgc tatgtttgct atgccttggt actggcactt
     2521 ggtggtcggc ggcttcgcca tcggtatgct gtttatggcg accgaccccg tttccgcttc
     2581 ctttaccaat gtcggcaaat ggtggtacgg cgcgcttatc ggcgtaatgt gcgtgttaat
     2641 ccgcgtggcc aatccggctt accccgaagg catgatgttg gcgattctgt ttgccaacct
     2701 gtttgccccg attttcgact atttcgtcgc acaagcgaac atcaaacgca gaaaggcgcg
     2761 cagcaatggc taagaaattc gataaagaca gcttcagcgg cacgctgatt gtcgtgttgg
     2821 cggtcagcct gatttgctcg gtcatcgttg ccggtgcggt cgtcggcttg aaacccatcc
     2881 aagaaaaaca aaaactccaa gacaaacaag gctatatttt gagcgtggcc ggtttgatgg
     2941 ataaggacac cgacatcggt aaaacctttg ccgagcgtat cgagcaacgc gtggtcgatt
     3001 tggcgaccgg cgaatatgtg aaagacgcgc cgaaagactt cagcgcgcgc atcgcaggca
     3061 aagaccccgc gcaaagcatc cgcatcaaac ctgaagacga tttggcaggc atcaaaagcc
     3121 gcgccaaata caccgaggtt tatttggtaa aaggcgaaga cggcaaaatc gggcaaatca
     3181 tcctgccgat gcacggcaac ggtttgtggt cggtgatgta cggcttcgtc gccatccgac
     3241 ccgacggcaa caccatcaac ggcattacct actacgaaca aggcgaaacc ccgggcttgg
     3301 gcggcgaaat cggcaatcct ttgtggcagc aaaaattcgt cggcaaaaaa ttgtttgacg
     3361 gacaaggcaa actcgccctg cacgtgggca aaggcgcggg ttcggacaaa gaacacggcg
     3421 tagacgccct ctccggcgcg tcgctgactt ccaaaggcgt gcaaggttcg ttcgcctact
     3481 ggttcggcga aaacggctat atcccctacc tgaacaaatt gaaatcagca ggagtgcaat
     3541 aatggctgat atgaaacgct tgaaacattt gatgttctca ccctttatcg acaacaaccc
     3601 gattgccttg caggttttgg gtatttgttc ggctttggcg gttaccacca aacttcagac
     3661 ggccatcgta atgggtattt ccgtagcttt ggtaaccggt ttttccagct tcttcatttc
     3721 gctggtgcgc aactacatcc ccaacagcat ccgcatcatc gtgcaaatgg cgattatcgc
     3781 gtcgctggtt acgctggtcg accaactgct tcaggcattt gcctacgaat tgtccaagca
     3841 gctttccgta ttcgtcggcc tgattattac caactgtata gtgatgggcc gtgccgaagc
     3901 atttgcgatg aaagagccgc cgctggaaag cctgatcgac ggcatcggca acggtgcggg
     3961 ctacgggatg atgctgcttg tcgtcgccac cgtccgcgaa ctgatcggct caggcaaact
     4021 tttaggttat accgtctttc aaaccgtaca ggacggcggc tggtatcaga ccaacggcct
     4081 cttcctactc gcccccagcg cgttcttcat catcggcttt ttgatttggg gactgcgtac
     4141 ttggaaaccc gaacaggcgg aggaataagc tatggaacac tatttgagcc tcttcatcaa
     4201 atccgtcttc attgaaaata tggcgctatc cttctttttg ggtatgtgta cgtttttggc
     4261 ggtatccaaa aaagtatcca ccgcattcgg tttgggtgtg gcggtaattt tcgtactcgg
     4321 gctgtccgtc cccgccaacc aattggttta ctcgctgctc aaagacggcg cgattgtcga
     4381 aggcgtggat ttgacctttt tgaaattcat cacctttatc ggcgtgattg ccgctttggt
     4441 gcagattttg gaaatgttct tggacaaatt cgtccccgcc ctctataacg cgctgggcat
     4501 ctacctgcct ctgattaccg taaactgcgc gattttcggc gccgtatcgt ttatggcgca
     4561 acgcgaatac gacttcggcg agtccgtcgt gtacggcttc ggcgcgggtt tgggctggat
     4621 gttggcgatt gtcgctttgg cgggcattac cgaaaaaatg aaatattcgg acgctcccaa
     4681 aggcctcaaa ggcttgggca ttacctttat cgccgccggc ctgatggcaa tggcgtttat
     4741 gtcgttctcc ggcatccagt tataagaaag ggaccggtat ggagattatt ttaggtatcg
     4801 tgatgtttac cgtcatcgtc ttagctttgg cactgatgat tctgtttgcc aaatccaagt
     4861 tggtcagcga aggcgacatc accatcaaag tcaatgatga aaaagaactg actatgcccg
     4921 ccggcggcaa actcttgggc gcgcttgcca gccaaggcat ctttgtcccc tccgcctgcg
     4981 gtggcggcgg ttcgtgcgga caatgccgcg ttgtcgtgaa aagcggcggc ggcgacattc
     5041 tgccgaccga gctgtcccac atcagcaaac gcgaagcacg cgaaggctgc cgtttgtcct
     5101 gccaagtcaa tgtcaaaacc gacatggaca tcgaagtacc cgaagaagtg ttcggcgtga
     5161 aaaaatggga atgcaccgtc atttccaacg acaacaaagc aacgttcatt aaagaactca
     5221 agcttgccat tcccgaaggc gaagaagttc ctttccgcgc cggcggctac attcaaatcg
     5281 aagccccgcc gcacaccgtt gcctacaaag acttcgacat tcccaaggaa tatcacgaag
     5341 actgggacaa atacaatctg tggcaatacg tttccaaagt ggacgagccg attttgcgcg
     5401 cctactctat ggcttcgtat cctgaagaaa aaggcatcat tatgctgaac gtgcgtatcg
     5461 ccacgcctcc cccgcgcgta cccgatgcgc ctccgggaca aatgtcgtcc tacatctggt
     5521 cgctcaaacc cggcgacaaa gttacgattt ccggcccgtt cggcgaattt ttcgccaaag
     5581 acaccgatgc cgaaatggta tttatcggcg gcggtgcggg tatggccccg atgcgttccc
     5641 acattttcga ccagttgaaa cgtttgcact ccaaacgcaa aatcaccttc tggtacggcg
     5701 cacgctccaa acgcgaaatg ttctatgtcg aagacttcga ccaactcgcg gcagaattcc
     5761 cgaacttcac ttggcacgtc gccctgtccg acccattgcc tgaagacaac tgggacggct
     5821 acacgggctt catccacaac gtggtttacg aaaaccacct gaaaaaccac gaagcaccgg
     5881 aagactgcga attttatatg tgcggcccgc cgattatgaa ccagtccgtc atcaaaatgc
     5941 tcaaagactt gggcgtggaa gacgaaaaca tcctcttgga cgatttcggc ggttaaatat
     6001 tcggcttcaa acacaaaagg aagaaacccc ggtttcttcc ttttttatcc gatgccgtcc
     6061 gaacaccctt cagacggcat ccgcccatac ccgtatcaat atcgtttttt taccctgcgg
     6121 cggatgtgcg gctaaatatt ttgacgaaaa aacaaagaag tatacttctt tttagttatg
     6181 gttgattcca taactaaaaa agtatcgatc aacccatttt acgaggtacg gataatgtct
     6241 gacagctttg aaaacagccc cgaatacgat gattggattg aatcgggcgg tcgtgatgaa
     6301 gattacgaat actactacaa caaatggcaa cgcaggacgc gctgaccatt aaaccctgac
     6361 aaaccggttt gtaaaacacc ggtttgtttt ttattaccgc cacccaaacg acgggcgatg
     6421 ccgcccgaac gcccttcaaa cggcatcggc atagaaacat accggccgtt ttgcgatata
     6481 attcaagcca tttgccaaaa ccgtcaaaca ctatgccgtc tgaaacacgc ctgccgaacc
     6541 ttatccgcgc cttgatattt gccctgggtt tcatcttcct gaacgcctgt tcggaacaaa
     6601 ccgcgcaaac cgttaccctg caaggcgaaa cgatgggtac gacctatacc gtcaaatacc
     6661 tttcaaataa tcgggacaaa ctcccctccc ctgccaaaat acaaaagcgc attgatgatg
     6721 cgcttaaaga agtcaaccgg cagatgtcca cctaccagac cgattccgaa atcagccggt
     6781 tcaaccaaca cacagccggc aagcccctcc gcatttcaag cgatttcgca cacgttaccg
     6841 ccgaagccgt ccgcctgaac cgcctgactc acggcgcact ggacgtaacc gtcggccctt
     6901 tggtcaacct ttgggggttc ggccccgaca aatccgttac ccgtgaaccg tcgccggaac
     6961 aaatcaaaca ggcggcatct tatacgggca tagacaaaat cattttgcaa caaggcaaag
     7021 attacgcttc cttgagcaaa acccacccca aagcctattt ggatttatct tcgattgcca
     7081 aaggcttcgg cgttgataaa gttgcgggcg aactggaaaa atacggcatt caaaattatc
     7141 tggtcgaaat cggcggcgag ttgcacggca aaggcaaaaa tgcgcacggc gaaccgtggc
     7201 gcatcggtat agagcaaccc aatatcatcc aaggcggcaa tacgcagatt atcgtcccgc
     7261 tgaacaaccg ttcgcttgcc acttccggcg attaccgtat tttccacgtc gataaaaacg
     7321 gcaaacgcct ctcccacatc atcaatccca acaacaaacg acccatcagc cacaacctcg
     7381 cctccatcag cgtggtctca gacagtgcaa tgacggcgga cggtttatcc acaggattat
     7441 ttgttttagg cgaaaccgaa gccttaaggc tggcagaaca agaaaaactc gctgttttcc
     7501 taattgtccg ggataaggac ggctaccgca ccgccatgtc ttccgaattt gccaagctgc
     7561 tccgctaaac atacaaaccg attaggaaac accatgaaaa ccctgctcct caccttcggc
     7621 atcttcctga ccgtcatcat cggtatggcg gtcggctata ttttctccaa acgcaccatc
     7681 aaaggcagtt gcggcggcat taccgcttta ggtatgaaaa aaatgtgcga ctgcgacaca
     7741 ccttgcgaca ccctgcaaaa aaagctggat gaagaaaaac aggcaggcgg catacgggtt
     7801 gaccgttaac gccgaaacaa aatgccgcct gaagcccggc cggcgggttc agacggcatt
     7861 gccgctttac ttcgatcggt ttatacgcaa aacgcgcttc aggaaataaa tcagattatt
     7921 ccccagacgc cttcaggctt ccttctgtgc caccgtaacc atagccgggc gcaacacacg
     7981 gtcggacagg gtataaccct tcttcatcac accaaccacg gtattgggtt cctgctcact
     8041 ggccaccgcc tgcatcgcct gatggatatt cggatcgagc ttatcgcccg ctttaggatt
     8101 gatttccttg atttgcgtgg catcaaacgc cttctgcaac tcattcaaag tcatctgcac
     8161 gcccattttc agcgcatcga aattaccgct ctgatccaaa agcgccattt ccagataatc
     8221 cttgaccggc aacatttcca cggcaaactt ctgtccggcg aacttgtgcg tatcggcaat
     8281 ttcctgctgg tggcggcggc gcaggttttg ctcgtttgcc aaagcgcgca gttgttcgtc
     8341 tttcaactgc gcttccaact cggcaatccg cgcctgcaaa tcctcataag ccggctcggc
     8401 ggcagcctgt tcctgtacac cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc
     8461 cacattttca accgcttctt cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc
     8521 ctcaaaacaa attggaaatc aaaatccggt tattacccga gcaagataag gacattttca
     8581 agaaacttca agcaaaggca ggaaatttca catccgccgc cgaataccct accgcaaaaa
     8641 ccatataaac ggtaagatac cgccgacctt atgcttttca gacggcatcc attatgaaaa
     8701 aaaaccattt gaacacaacc ggtttcgacc tctggcacac catccgcgaa gaaaccgcgg
     8761 ccgctgccgc cgccgaaccg atgctggcaa gttttttgca ccaaaccgtg ttgcgccacg
     8821 agtccctcgg ctccgtcctt gcctaccacc tttccagcaa actcggcagc ccgattatgg
     8881 acgtgcgcgc gctgtttgaa atttaccagc aggcattggg cagcgacacc caaatcagca
     8941 aatgtgtcga agcagactta aaagccatct acgaacgcga tcccgcctgc gacgaatatt
     9001 cgctgccgct tttatatttc aaaggcttcc acgcgattca ggcacaccgc atcaaccacc
     9061 ggctgtatct cgacggacgc aaaacgctgg cgtatttcct acaaaaccgt atgtccgaag
     9121 tattcggcgt ggacatccat cccgccgccc gtttaggata cgggctgatg ctcgaccacg
     9181 ccaccggctt tgttgccgga gaaaccgccg tgttgggcaa caatatttcg attttgcacg
     9241 gcgtaacgct cggcggttcg ggcaaagaag gcggcgaccg ccaccccaaa atcggcgacg
     9301 gcgtgatgat cggcgcaaac gcctcgatat tgggcaatat ccgcatcggc agcaatgcca
     9361 aaatcggcgc gggcagcgtc gtggtttcag acgtgccgcc gtccatcacg gttgtcggcg
     9421 tacccgccaa acccgtggcg cgatcgctca aaaccccgtc ggcggatatg gatcaaaata
     9481 tccagtttgc cgaaatcgac tttatgattt gaatatccgc accaatgccg tctgaaacgc
     9541 caaacgggct tcagacggca ttgcccgctt caagcctgcg ccttaatatg ccgccgatgc
     9601 cggcacttat cagtcggaca aaatgccttt tattcgatac gcccgattcc cacgcaaccg
     9661 gaaataaccg gtttccttgt aaacgggcgg agtcgggcaa tgattcggac aaagcctatt
     9721 tcaaaagcaa aatcagcagc aaaacaaccg ctatcgctgc cagccacagg ctttgccgct
     9781 gctgcacttt gaccaaatgg atataggcat cgcgcatttc ctgccggcgc gcctcatcga
     9841 ccaaggcatt gattttgcgc ggcagggaag ggatgatttg cgcccagtct ggggcttcgt
     9901 ttttgaggtt gcgccaaagg gctttgggac cgacctgccc gttcatccat tttaccaaaa
     9961 acggtttggc ggttttccac aagtccaaat cgggatcgag ttggcggccc aagccttcga
    10021 tgttgagcag cgttttttgc agcaatacca gctgcggctg gatttcgaca ttgaagcggc
    10081 ggctgacttc aaacaggcgc atcagtacca agccgaagga aatctgcgaa atcggtttgt
    10141 tgaacacagg ctcgcacacg gcgcgaaccg ccgcttccaa ctcttccgca cgcgtgtcgg
    10201 caggcaccca gcccgattcg acgtgggcgg tggcgacacg tcggtaatcg cggttgaaaa
    10261 aggcgaggaa attgatggcg agatagcgtt tgtcgtaatc ggtcagcgta ccgacgatgc
    10321 cgaaatcaag ggcgatatag cggttgtcgg cggcaaccaa aatattgccg gggtgcatat
    10381 ccgcgtggaa aaagccgtcg cggaaaactt gcgtgaaaaa gatttccacg ccgtaatcgg
    10441 caagtttgtg caaatcgatg ccgtctgctt tgagtttggc gatgtcggaa accggcgtgc
    10501 cgtccatcca ttcaatcgtc agcacgtcgc ttgtgcagta gtcgtaaaac accttgggca
    10561 caatcagcat attgctgttt tggaaattgc gcccgagttg tcctgcattg gcggcttcgc
    10621 gcatcaaatc caattcgtcg tgcaggtatt tgtcgaactc cgccaccact tcgcgcggct
    10681 tcagacgctt gccgtctgaa aacaagcgtt cgacccaagc cgcgccgaaa cgcatcagcg
    10741 acaagtcttg ttcgataagg ggcaagaggt tggggcgcaa aactttgacc gccactcgtt
    10801 cgcccgaatg caggcgggct ttgtgtactt gggcgatgga cgcgctggcg acgggctcgg
    10861 tttcaaattc cgcatacagc ttttcgatgg gctgacccag cgatttttcg atttgttcac
    10921 gcgaaagccg cgcgtcaaaa ggcggcactt tgtcttgcag ctttgccagt tcgacggcgt
    10981 aatcgtgcgg aatcaaatcg gggcgtgtgg acaaaacctg cccgaacttg atgaaaatcg
    11041 gccccaggct ttccaaggca aggcgcagac ggacggcagg cggttcgttt ttcaatttgg
    11101 acgactgcgg catcattttc agcagagcgc atatccaacc gctacccatc agcgaaacgc
    11161 acagacctgc cagccgatag cggtaaaaag tcccgacaat gaccgtcagg cgtttcaacc
    11221 atttcatata taaaatcccg aatacagcat ttatcggaca atcaaaaaat cttaccgagc
    11281 agatagccca aaaaggcaat cagcaacccg aaaaccagcg gtttgatatt gcggggcggc
    11341 tcgggttgcg aagatgcgga aatatcatct tcccgcccgc cgtgaagttc aagatattcg
    11401 ggcatttccc catccgcgcc tcctccttcg ccctccccct cctccggcgg cacgaccgtt
    11461 tccaactcca tatacgggta ataatatcca aacccaaata gctgaaataa tcatgcaact
    11521 cttcccgttt cgcccaagtg catttttcct caaccagcac ttgcggtttg gaatcgagcg
    11581 attcgatcaa tccttccgcc ttttcttcgc cgagattctc cttaaggcac tcccggataa
    11641 gcccgcgatc cacttggggc gggtaggaaa catatacatc gtacaaatcg tttcggtaat
    11701 ctttactcat ctcaatcata agggaacaaa tacgcctgat accgttccgc ccgttccttt
    11761 cgggaaaccc gattcatctt caagcaatcg gattatatgt aaattcatcc cgataatata
    11821 ttttccaaaa gtaaaaatgc cgtccgaacg ttcagacggc ataacacaaa ccagccggtc
    11881 acacggaaaa cgacgatccg caaccgcagg ttgtttccgc gttcggattg cggatgacga
    11941 actgcgaacc ctgcagactt tccgtatagt cgatttccgc accgaccaga tattgatagc
    12001 tcatcggatc gaccaaaaag accaaaccgt ttttctcaat ttcaaaatcg tcgtcgtttt
    12061 tgatttcgtc aaaagtaaat ccgtactgga aacccgaaca gccgccgccg ttgacaaaaa
    12121 cccgcaattt caaatcggga ttgttttctt cggcaatcaa atcggcaact ttggcacagc
    12181 agctgtcagt aaaaataata gggctttcgt ccgacatagt gttattcctt aatataatat
    12241 tttctgcatt ctctctcaac cccccttcaa aggcaagcag ggcttcgcct ttgaaaaagg
    12301 aattgtccca atagataagt ttgaccgcct gcaatttcaa gccccaaact caaaccgtcc
    12361 tgccgaacca ctcgacacgc ccgataatct ccacatcatc ggtcaagtcg ttcaaattga
    12421 tttcaaaagc aggataagcc tcgttggcgg aaatgacatt gataatcccg ccggggacga
    12481 tttgcaggcg tttgaccaac agattttcat taatccgcaa cacatacagt ccgtctctcg
    12541 gcgtattttc cccatgattg accaaaatcg aatctccgtc attcaaaacc ccctccatcg
    12601 aatccccctt gacggaaatt acagacaggt ttttcgtatc tcgggtaaca taattctcaa
    12661 tccaatgccg tctgaacgcc attgtaaata ccggttcctc atgaccgaca aactgcccgt
    12721 atcccgcagc cgcccgaata tcatatctcg gcacaaagac aaactcgtcc gtatcaactt
    12781 catttcccaa ggtatcgtaa gcaagggatt tttttggggc ttcatccgga aacggattac
    12841 cctcccctgt cagcagccaa tcgatactac accccttcaa ctgcttaatt tttttcaatg
    12901 tttcagactt cggcagaccg ccttcattcc atattctgct gaagcccgca atcgtcattt
    12961 caatatcgga tgcgattttt gcctgcctcg cttcactttt ccataaaaaa accagtctgt
    13021 ctttaaaggt ttccataaat atccttccgc tccccttcat tcaatcatct aaagtaaaca
    13081 tgaaatatat tttacattag ctatcccaaa aacggaataa ctatgataaa ctaaatttaa
    13141 attatgattt ttcttatcca atacataatt tttcagattt tccataggtt tgatcccgaa
    13201 ttgacagaaa aaggatagaa aaatgaagaa aaccagcaaa tatcttatct atactgcggc
    13261 atttacctca ttctgctttg ccttccaaga aaaccgttct gaagccaaac agcccgacat
    13321 cactttatcc gcatccctgt gcgaacaatt caacatgctg aacgccaaag atatggatgc
    13381 agaacaagtc tccctttcca aagaatgcga catcatcgag tcttcacacg actgggaaaa
    13441 agagtacggc aacttgaacg aacaggaaat gctcgccggc gtcgtctatg aataaacctg
    13501 cctgccattt gaaacattat gcttgaatgc attggagcca aatgtattaa atcaaatata
    13561 aaaccaatat attcataaag ttatatactt atagccatgc tgtagcttga aacagcccgc
    13621 cgcccgccct atttacagcc catcgggaca aaatgttcca acatatttct caaaataagc
    13681 aaaatcaaat aggagtatcc acatggcaaa agtaatcggt atcgacttag gtacaaccaa
    13741 ctcttgtttg gccatttccg aaaacggtca aaccaaagtc atcgaaaacg cagaaggcgc
    13801 acgcaccacg ccgtccatta tcgcttattt ggacggcggc gaaatcctcg tcggcgcgcc
    13861 tgccaaacgc caagcggtaa ccaacgccaa aaacactatt tacgccgcca aacgtttgat
    13921 cggtcacaaa tttgaagaca aagaagtcca acgcgacatc gaatctatgc ctttcgaaat
    13981 catcaaagcc gacaacggcg acgcatgggt aaaagcacaa ggcaaagagc tgtctcctcc
    14041 tcaaatttcc gcagaagtcc tgcgtaaaat gaaagaagcc gccgaagctt acttgggcga
    14101 aaaagtaacc gaagccgtga ttaccgtccc tgcctacttc aacgacagcc aacgtcaagc
    14161 caccaaagac gcaggccgta tcgccggttt ggacgtaaaa cgcatcatca acgagccgac
    14221 cgcagccgct ttggcattcg gtatggacaa aggcgacaac aaagaccgca aaatagccgt
    14281 atatgacttg ggcggcggta ccttcgatat ttccatcatc gaaatcgcca acctcgacgg
    14341 cgacaaacaa tttgaagtat tggctaccaa cggcgatact ttcttgggcg gtgaagactt
    14401 cgaccaacgc ttgattgact acatcattga cgagtttaaa aaagaacaag gcattgattt
    14461 gaaacaagac gtaatggctc tgcaacgtct gaaagaagct gccgaaaaag ccaaaatcga
    14521 attgtccagc ggccagcaaa ccgaaatcaa cctgccgtac attaccatgg acgcaaccgg
    14581 cccgaaacac ttggcaatga aaattacccg cgccaaattc gagagcctgg ttgaagacct
    14641 gattgcccgc tctatcgagc cttgccgcac cgcattgaaa gatgccggct tgagcaccgg
    14701 cgacatcgac gacgtgattt tggtcggcgg tcagtcccgt atgccgaaag tacaagaagc
    14761 cgttaaagac ttcttcggca aagaaccgcg caaagacgtg aaccctgacg aagccgttgc
    14821 cgtaggtgca gcgattcaag gcgaagtatt gagcggcggc cgcagcgacg tattgctgct
    14881 ggacgtaacc cctctgtcct tgggtatcga aaccatgggt ggtgtgatga ccaagctgat
    14941 tcaaaagaac actactattc cgactaaagc gtctcaagtg ttctctactg ccgaagacaa
    15001 ccaaagcgca gtaaccatcc acgtactgca aggcgaacgc gaacgcgctt ctgccaacaa
    15061 atctttaggt cgattcaact tgggcgacat cgcacccgca ccacgcggta tgccgcaaat
    15121 tgaagttact ttcgacatcg acgccaatgg tatcttgcac gtttccgcca aagacaaagg
    15181 cactggcaaa gcggccaaca ttaccatcca aggttcttca ggtttgagcg aagaagaaat
    15241 cgaacgcatg gtgaaagatg ccgaagccaa tgccgaggaa gataaaaagc tgactgaatt
    15301 ggtcgcttcc cgcaaccaag ccgaagccct gattcactcc gtgaaaaaat ctttggcgga
    15361 ctacggcgac aaactcgacg ctgccgagaa agaaaaaatc gaagccgcgc tgaaagaagc
    15421 cgaagaagcc gtgaaaggcg acgacaaaac cgccatcgat gccaaagccg aagcactggg
    15481 tacagccagc caaaaactgg gcgaaatggt ttacgcgcaa gcgcaagccg aagcccaagc
    15541 cggcgagggc gcacaagcca atgcttctgc aaagaaagac gatgatgtcg tagatgccga
    15601 ctttgaagaa gtaaaagacg acaaaaaata attgatgccg tctgaaaaaa acgcgaacca
    15661 ttcggttcgc gtttttttca attgagataa aagacaatag catgacagag attccaaatt
    15721 catctaccgt gatattccca agcccaagtt ccaactgttg catcggttat ctggaaattt
    15781 ttcatatcat tttcttattt actta
//
LOCUS       DAWTCI010000050        15003 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWTCI010000050 DAWTCI010000000
VERSION     DAWTCI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.050"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(41..211)
                     /locus_tag="WM481_001458"
     CDS             complement(41..211)
                     /locus_tag="WM481_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HFA9080757.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
     gene            complement(344..1426)
                     /locus_tag="WM481_001459"
     CDS             complement(344..1426)
                     /locus_tag="WM481_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HFA9080758.1"
                     /translation="MNAQTLLANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGA
                     VGIEAEGGNGLGLATRIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDKYLA
                     DILQGKVLAGTGMSNTVKHLAGIEKHNLQAECVDGGYKVNGALPWVSNIGEDHIWANT
                     AQIGGGYVMFITGGQREGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPEQFA
                     GYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNSYLDDGYDSLKARLDGAW
                     AETERLAGLAWDNTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQRRVG
                     EAMFVAIVTPAIKHLCKEIAAIEAAK"
     gene            complement(1564..2112)
                     /locus_tag="WM481_001460"
     CDS             complement(1564..2112)
                     /locus_tag="WM481_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFA9080759.1"
                     /translation="MARLTVHTLETAPEAAKPRVEAVPKNNGFIPNLIGVLANAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIRIIAARTNQCGFCVAGHTKLATLKKLLSEQSLNAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNRQQAVEVVMGVALA
                     TLCNYANNLAQTEINPKLQAYA"
     gene            complement(2252..2623)
                     /locus_tag="WM481_001461"
     CDS             complement(2252..2623)
                     /locus_tag="WM481_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFA9080760.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     MTGAMFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGG"
     gene            complement(2808..4499)
                     /gene="dld"
                     /locus_tag="WM481_001462"
     CDS             complement(2808..4499)
                     /gene="dld"
                     /locus_tag="WM481_001462"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFA9080761.1"
                     /translation="MNASQLISSLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDMPEEILTNLQGHHYQKKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKVSFLPKHFSDKAMQFVSKFLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFSHHGGAFFECNAEETQAAMLHRFAVASAAIRYRSVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNGCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            complement(4596..5015)
                     /locus_tag="WM481_001463"
     CDS             complement(4596..5015)
                     /locus_tag="WM481_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080762.1"
                     /translation="MKKLLMITLTGMLAACSTGVNVGRLMVEMPQGERSVVVQVPATN
                     NPLSDAVAVGMIKTSGSPSASNMIEMLGADNINVGVAGGSQMFNKATALYSLNHAKKV
                     GNNVSVYMTGDSESDKADLENAANAKNIKLHYFFNQK"
     gene            complement(5238..5334)
                     /gene="ffs"
                     /locus_tag="WM481_001464"
     ncRNA           complement(5238..5334)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WM481_001464"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5595..9428
                     /locus_tag="WM481_001465"
     CDS             5595..9428
                     /locus_tag="WM481_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HFA9080763.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     QQVDVLIAAAEKAVERFDGSFDETRQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAVPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKARWDWLLAHLDAPLADYKSRYGTA
                     VHAAPEAKDDESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLTDEDLQPFWNYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIHNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYSDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            9505..10506
                     /locus_tag="WM481_001466"
     CDS             9505..10506
                     /locus_tag="WM481_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HFA9080764.1"
                     /translation="MIDGQTTEPKQKTRIIPAPMRGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTGKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIHDTVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSTWIGQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEMDV"
     gene            complement(10567..10749)
                     /locus_tag="WM481_001467"
     CDS             complement(10567..10749)
                     /locus_tag="WM481_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080765.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPKAGEKRQ"
     gene            complement(11312..12456)
                     /locus_tag="WM481_001468"
                     /pseudo
     CDS             complement(11312..12456)
                     /locus_tag="WM481_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(12781..13257)
                     /gene="greA"
                     /locus_tag="WM481_001469"
     CDS             complement(12781..13257)
                     /gene="greA"
                     /locus_tag="WM481_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFA9080766.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPAEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            13648..14949
                     /gene="aroA"
                     /locus_tag="WM481_001470"
     CDS             13648..14949
                     /gene="aroA"
                     /locus_tag="WM481_001470"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFA9080767.1"
                     /translation="MTESVRLPAASLKPSTVALPGSKSISNRTLLLAALSDNVCEIHS
                     LLKSDDTDRMLEALDKLGVQIEHLAEGRLKVHGTGGRFPNRSADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGNEHYPPLHIGKRQDCGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     EGYRVFKIPADAHYHAPEHLHVEGDASGASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKGRAVQAFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLISLLGVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
BASE COUNT         3394 a         4133 c         3970 g         3506 t
ORIGIN      
        1 cggaacacgc cgagggattt tccaaggggt gcggctgccg ctatatcacg aaatccaaca
       61 ggtagaaatc ttctttgccc acgccgcatt cggggcattt ccagtcgtcg gggatgtctt
      121 caaactttgt cccgggggcg atgccgtgtt ccgggtcgcc gagttcttca tcgtaaatcc
      181 agccgcaggg gccgcacata tattgagcca tttgtgtttc cttgtttgta ccgtgctttt
      241 ttgcagcggg ccgccggggt ttggttcgga tgccgtctga agcgtgccgc cgcatctgtc
      301 ccgccttggg cggtttgtgc ttcagacggc attttccgcc tgtttatttt gcggcttcga
      361 ttgctgctat ttctttgcag aggtgtttga ttgcgggggt tacgatggcg acaaacatcg
      421 cttcgccgac gcgcctttgg gcgggactcc gcatcaggta gcctttcgcg ccggaatgca
      481 gggcggcgga ttgcgcagcg gcaagggcca gtacggcggc ggcttcgcgc agcttgaggg
      541 tggcgaggtt gtcgggcgtg ttgtcccacg ccaatccggc gagccgttcg gtttctgccc
      601 acgcgccgtc cagccttgct ttgaggctgt cgtagccgtc gtcgagatag ctgttgactt
      661 ctgcattgac gatgttggcg aggcggatga tgccgaggct gccgtctatc acgcccgcgc
      721 cgatgccgat ttgcaggagg ataaagcctg ctttgatgct ttggatgtag ccggcaaact
      781 gttcgggcgc ggcgatgatg tcttcgtcgg ggataaatac gtctttgaag ttcaggctga
      841 aggtgcgcgt gccttcgagg gcgcaaaatt cggggcagtt ttgcaggctt acgccttccc
      901 tttgcccgcc tgtgatgaac atgacgtagc cgccgccgat ttgggcggta ttcgcccaaa
      961 tgtggtcttc gccgatgttg gacacccacg gcagcgcgcc gttgactttg taaccaccgt
     1021 ccacgcactc ggcttgaagg ttgtgttttt caatgccggc aaggtgtttg acggtattgg
     1081 acattccggt gcccgccaat actttgcctt gcaggatgtc ggcgaggtat ttgtctttga
     1141 cggcttggtt gggcgtttgg tgcagatacc acgcgcaagc cgcctgacac cacgcgctga
     1201 aggaggtcgc gccgcattct ttgccgattt cgcgcaagac ggcgattcgt gttgccaagc
     1261 ctaagccgtt gccgccttcg gcttctatac cgactgcgcc gattgcgccg agttcgcgca
     1321 taaatgcttc ggggtagtat cctttgcggt cgatgtcgtc gacgaggggt ttgagcttgg
     1381 ttttgacgag ttcggcaacg ttggcaagca gggtttgggc gttcatcttt gttccttaag
     1441 gtttttgggg ggaaatcggg ggcacgcctg atgcgcggct gcttttcccg ccgcttgggc
     1501 ggcttgtttt tgtttgaggg ggtgtttttg aaaaaccgcc tgcattcagg cggttttgtt
     1561 tgcttaggcg tatgcctgca atttggggtt gatttcggtt tgggcgaggt tgttggcgta
     1621 gttgcacaaa gttgccaagg ctacgcccat tacgacttcg actgcctgct gccggttgta
     1681 gcccgcatcg aaaaatgctt tgagttcgtc gtcggatact gcgccttttt tcgccattac
     1741 ggcttgggtg aaggcggcaa gcgcgccgag tttggcatcg tcaaattcac ctgccgccaa
     1801 agcgcgggcg gcattgaggg attgctcgga caggagtttt ttcagggttg cgagtttggt
     1861 gtgccctgcc acgcagaagc cgcattggtt ggtgcgtgcg gcaatgatcc ggatgacttc
     1921 gacttcgccg gcggtcaggc tgttggcggc gttgagcttg ccgacttctt ggtaaaacgc
     1981 caaggcttcg ggggcgtttg ccaatacgcc gatgaggttg gggataaagc cgttgttttt
     2041 gggtacggcc tctacgcgcg gtttggcggc ttcgggggcg gtttcgaggg tgtgtacggt
     2101 taaacgtgcc attttctttc cttgttcgca aatattgggg acgggcgcat ggtacgcatt
     2161 tcggaacgga ataggaaaga ctgtgccaca aacaaaaagt tataagaaat gccgcctgaa
     2221 gcctttcaga cggcattgcc gggaaacacg cctacccgcc cccgcgtccg aaatattgcg
     2281 ccaatgcggc ttgggggttg gcggcttcga ctgtttcggg caattcggta tagccgcgcg
     2341 ataaaaggtg gcgtgcataa aggctgttgc cctttgcgcc gaaccagacg gaaaccgcca
     2401 agcccaatac gttaaacgcc gagtctgtgg cgggcgtgtc gccgtaaacc agttgggcaa
     2461 acaccgccaa cacgaacatc gcgcccgtca tccccagccc caaaccccac attcttttga
     2521 aacacgccca cagcgtgccg aacaaaagac ccggccacga ccagccttgt ttgacggctt
     2581 ggggcggcag ggcgggatgg gtgtagattt tgtatggttt catcgtgttt ccttttcggt
     2641 tgaaaccctg ccctttggga aggtaggatc agactttatt tgtgagacgc gcgcccgtgt
     2701 gcagacatac ggcttgaaca tgcccgtcgg ggaagacaga tccgacaatg ccgtctgaac
     2761 ggtgttttgc cttcagacgg cattgcctgc cttcaaagcg gacgcgctta ttccgcccag
     2821 tttttctttt tgctggtttt gccgataccc gggttgaagc tgttggtcgg gtcgagtttg
     2881 cggtaaaact gtttgagcgc gggcttggct tcatacaaat ggccgacgtt gtgttcggcg
     2941 ggatattgcg cgccgcgttg gtctaagaga tgtagcattt cgtgttccaa cgccatacag
     3001 ccgttgcctt ttttgatgat gtaatcctga tggaaaacgt ggcacataaa atgcccgtaa
     3061 tacaatttat ggatgatttt attgtcgatt tccggcggca gtttttcaaa ccagtcgcgg
     3121 tcgtcgcggc gcagggcgat atccagcgcg accaagtcct ccacttcgtc gtcgtgcacg
     3181 gaacggtagc ggatggcggc ggaagcgacg gcgaaacggt gcagcatcgc ggcttgggtt
     3241 tcttcggcgt tgcactcgaa aaacgcgccg ccgtggtgtg aaaaatattc ttttaagaac
     3301 gcgcgcgcct catccacgcc ttttccgccc attttcagaa tcaggtggtg ttcgtatttg
     3361 tcgcggtaat cgcgcatgga tttgggcagg tggtcgggca ggaatttgct gacgaactgc
     3421 attgccttgt cggaaaaatg tttgggcagg aagctgactt ttttgccgaa cctgtccacg
     3481 cgcgccttca agtcaaataa tttcggcagt tggtgcgtac cgaatttttt gatgacgtag
     3541 aacgtgtctt tgccgtacac gtcggcaatg tcgaaagcgt ggcggtggat gtattcgccg
     3601 gaaacgggca ggctttcaaa ttcgcccaag gcggcgcggc ggatgtcggt cagctcgttg
     3661 atgtcgttcg tgccgatgta gaacacggcg gtttgttttt cttgcgggaa ggtgtccaaa
     3721 cggacggcaa aaaccatcag cttgcccgcg cagcccgagg cttcgtaatg gcgggcgggg
     3781 tcggcattga aacgcgcggc ggtcggttcg tccacttggc ggacgtgttc gcaataggcg
     3841 tggtcatgtc ctttgcccgc gtcttgtttg atgtcttttt tctgataatg atgaccttgc
     3901 aggttggtca ggatttcttc gggcatgtcg cccaagtcta tgcccaagtg gttgaccagt
     3961 tccaacctgc cctcttcgtt gatttgggcg aacaacgcca tttcggtgta ggccgggccg
     4021 cgctgtacca acgcgccgcc ggagttgttg cacacgccgc ctaaaacgga cgcgccgatg
     4081 caagaagagc cgatgaccga atgcggttct cgtcccaaag gtttcagcag cagttcaagc
     4141 tggttcaggg tcgagccggg caggcagacg acttgttcgt tgttgttgat ggtttggatg
     4201 atgttcatcc gcatggtgtt cacaatcacg atgtcgcggt cgtaatcgtt gccgtcgggg
     4261 gtcgagccgc cggtcagtcc ggtattcgcc gcctgcgtaa tcacaatcac gtccgcttcg
     4321 acgcaggcct gcagaatttt ccacatttcc aaaatgcttc ccgggcgcac caccgccaac
     4381 gccttgcctt cgccgaaacg gtagccttgg cggtattgtt cggttttcgc ggggtcggtg
     4441 atgatgtatt tttcgcctac ggtttgggtc aggctgctga ttaattgcga ggcgttcata
     4501 acactctcct atcgggataa gcggcggcgc aaatcccggt acgccgccgt atgttcaatt
     4561 tggatatttt ggtgcggcaa gttgcacgct gcaaattatt tttggttaaa gaaataatgt
     4621 aatttaatat ttttggcatt tgccgcgttt tccaaatcgg ccttgtcgct ttcgctatcg
     4681 cccgtcatat agacactgac attatttccg actttctttg catggtttaa ggaataaagt
     4741 gcggtcgcct tattaaacat ttggctgcct cccgccacgc cgacgttgat attgtccgcg
     4801 ccgagcattt caatcatatt tgatgccgaa ggcgatccgg atgttttaat cattccgaca
     4861 gccaccgcat cggaaagcgg gttattcgtc gcgggaacct gcacgacaac agagcgttct
     4921 ccctgcggca tttcaaccat caaccggccg acattgacac ctgttgaaca agctgcaagc
     4981 ataccggtga gggttatcat tagaagtttt ttcatttgcc tactccttta gtctttaatc
     5041 aaaccgccct gttcgtcata taacttgaaa ttgaaccata cttccatttt tatgaagccg
     5101 gttcttcatt ggcctgtatg gatacattgt aatgatgcgt tatggcaaag taaaggaaat
     5161 gccgtctgaa cggctttcag acggcatcgg tgcgatacgg gaacgccgga acatcgaagc
     5221 tccggcgttt caaatagggc ggcggaccaa acccccggca ctggcgcatc ggagtgggct
     5281 gctggcttcc gcccctgacc cggtgttccg atttgccatg cggggagacc cgcctcggag
     5341 aaacggcatt ataacgggtt ttgtgaaaaa ctcaaccgtt ttgatacggt catacgccgg
     5401 aaacactgtc taaaacttat atccgataat attgtcaaca atttttcaaa gcgtcatttt
     5461 gtttctatga gggtatttcc tgtttcggca ttgaaaaata ttaagaatta aactacatta
     5521 ttgcctttca aaatttgtct gcaaccgacc gttcggacaa cactcaaata aaaacaacca
     5581 aacacggaca cgccatgacc acgactaccg ccccgcagcg tattcgggaa attccctaca
     5641 actatacttc ttataccgac cgcgaaatcg tcatccgcct gttgggcgat gaagcgtggc
     5701 aaatcctgca agacctgcgc ggtcaacgca aaacaggacg ttcggcgcgg atgttgtttg
     5761 aagtgttggg cgatatttgg gtggtcgtgc gcaatccgta tctggtcgat gatttgctgg
     5821 agcatccgaa acgccgcgcc gcgttggtac gcgaaatgcg ccatcgctta aatgaaatcc
     5881 gcaaacgccg cgacgacaat cagcaagtgg atgtgttgat tgcggcggcg gaaaaggcag
     5941 tggagcgttt tgacggcagc tttgacgaaa cgcgccaaaa gcggcggcag attttggaac
     6001 gcttaagcaa aatcaccaag ccgcacaaca tcatgtttga cggcttggct cgcgtaacgc
     6061 acgttacaga tgcaaccgac tggcgcgtgg agtatccgtt tgtcgtcgtc aatcctgata
     6121 ccgaggcaga gattgcccct ttggtgcgcg ccttaatcga attggacttg gttattatcc
     6181 cgcgcggcgg cggtacgggt tataccggcg gcgcagtacc tttggacgca aacagcgcag
     6241 tcatcaatac ggaaaagctg gacaagcatc gcggcgtgga atacgttgag ctggcaggct
     6301 tggacggcaa acatccgatt atccggtgcg gcgcgggcgt ggttacgcgg cgggtggaag
     6361 aaaccgcgca tcaggcgggc ttggtgttcg ccgtcgatcc gacttccgcc gatgcgtcct
     6421 gcgtgggcgg caatgtggcg atgaacgcgg gcggcaaaaa agccgtgctg tgggggacgg
     6481 cgttggacaa cctcgcctac tggaacatgg ttaaccctca aggcgaatgg ctgcgtatcg
     6541 aacgcgtgcg ccacaatttc ggcaaaatcc acgacgaaga aactgccgtg ttcgacgtgc
     6601 acacgctgga ttcagacggc atcaacatcg ttaaaaccga acgcttggaa atccccggac
     6661 acaaattccg caaagtcggt ttgggcaaag acgttaccga caaatttttg agcggactgc
     6721 ccggcgtgca gaaagaaggt acggacggca tcattaccgg cgttgccttc gtattgcaca
     6781 aaatgccgaa atacacgcgc accgtgtgta tggagttttt cggcacggtc gccaccgcca
     6841 cgccttctat cgtcgaaatc cgcgattttc tgctcgcaca cgacagcgtg cggctggcgg
     6901 gtttggagca tttggactgg cgttatgtcc gcgccgtcgg ctacgccacc aaagcggcgg
     6961 gcaaaggacg accgaaaatg gtcttgctgg cggacgtggt ttcagacgac gaagccgccg
     7021 tagaggcagc cgccgaacac atctgcgaac ttgcccgcgc ccgtgacggc gaaggcttta
     7081 tcgccgtatc gcccgaagcg cgcaaaacct tttggctcga ccgcagccgc accgccgcca
     7141 tcgccaaaca caccaacgcc tttaaaatca acgaagacgt ggtgattccg ctggagcggc
     7201 tgggcgagta ttcggacggc atcgaacgca tcaacatcga gctttccatc caaaacaaac
     7261 tcaaactctg tgccgctttg gagcaatatc tttcgggcaa actccccatc gacaaaatgg
     7321 gcaccgacct gccgaccgcc gaactgttgg gcgaacgcgg caaacacgcc ctcgcccacg
     7381 ttgccgccgt caaagcgcgt tgggactggc tgctcgccca tttggatgcg ccgcttgccg
     7441 actacaaatc acgctacggt acagccgtcc acgccgcgcc cgaagccaaa gacgatgaaa
     7501 gctgcttcac cgccttccgc gatttccgcc tgcgcgtgtc tgtcaaagca gacgtaatga
     7561 agccgcttgc cgaaatcttc agcggcaaaa ccgataccaa aattatccaa ggcttgggca
     7621 aaatccacgc caaaaccgta cgcagccgcg tctttgtcgc cctgcatatg catgccggcg
     7681 acggtaacgt ccacaccaat attccggtca actcagacga tgccgaaatg cttcagacgg
     7741 cctaccgttc tgtggaacgc attatgaaaa tcgcccgttc gctcggcggc gtgatttccg
     7801 gcgaacacgg catcggcatc accaagctcg aattcctcac cgatgaagat ttgcaaccgt
     7861 tttggaacta caaaaaccaa gtcgacccca agcacacctt caaccgccac aaactgatga
     7921 aaggttcgga cttacgcaac gcctacacgc cgtctttcga gctgttgggc gcggaatcgc
     7981 tgattatgga aaaatcagac ctcggcacga ttgccgattc cgtcaaagac tgcctgcgct
     8041 gcggcaaatg taaacccgtc tgctccaccc acgttccgcg cgccaacctg ctgtacagcc
     8101 cgcgcaacaa aatcctcggc gtgggtttgc tgaccgaggc cttcttatac gaagaacaaa
     8161 cccggcgcgg cgtttccgtc aaacattttg aagaactgat ggacatcggc gaccactgca
     8221 ccgtgtgcca ccgctgcgtc aaaccctgcc ccgtcaacat cgacttcggc gacgttaccg
     8281 tagccgtccg caactacctt gccgattccg gccacaaacg ctttgcgcct gcggcttcaa
     8341 tgggtatggc gtttttgaac gccaccggtc cgaaaaccat caaagccctg cgcgccgcga
     8401 tgatacagac cggcttcccc gcgcagaact ttgcctacaa aatcggcaag cttcttccga
     8461 tcggcacgaa aaagcaaaaa gccgaaccca aggcaaccgt cggcaaagcc ccgattaaag
     8521 aacaggttat ccatttcatc aaccgcccac tgcccaaaag cgtacccgcc aaaacaccgc
     8581 gctccttatt gggcatcgaa gacggcaaaa gcatccccat catccacaac cccgccgcgc
     8641 ccgaagatgc cgaagccgtg ttctacttcc ccggctgcgg ttcggagcgt ttgttcagcc
     8701 aaatcggact cgccgtccaa gccatgctct ggcacgtcgg cgtacaaacc gtcctgccgc
     8761 ccggctatat gtgttgcggc tatccgcaag acgctggcgg caacaaggca aaagccgaag
     8821 aaatgagcac caacaaccgc gtcgccttcc accgtatggc gaacaccctc aactacctcg
     8881 acatcaaaac cgtcgtcgtc agttgcggca cttgttacga ccagcttgaa aaataccgct
     8941 tcgaggaaat cttccccggc tgccgcatca tcgacatcca cgaatacctg ctcgaaaaag
     9001 gcgtgaaact cgacggcgtg aaaggtcagc aatacctcta ccacgacccc tgccacaccc
     9061 cgattaaaac catgaacgcc acccaaatgg ccagcagcct gatggggcag aaagtcgttt
     9121 taagcgaccg ctgctgcggc gaatccggta tgttcgccgt caaacgcccc gacatcgcca
     9181 cacaggtcaa gttccgcaaa caagaggaaa tcgagaaaaa cctcaaagag ctgccgcagg
     9241 gcgaacccgt caaaatgctg acctcctgcc ccgcctgcct gcaaggactg agccgctatt
     9301 ccgacgacaa caatatgcct gccgactaca tcgtcattga aatggcgaaa cacatcctcg
     9361 gcgaaaactg gctggatgag tttgtaaaaa aagccaacaa cggcggcgta gagaaagtgt
     9421 tgctataaca acggacacgg aaatgccgtc tgaacgccga aagccttcag acggcattgt
     9481 ttgaatcaaa tacctacccg aatcatgatt gacggacaaa caaccgaacc caaacaaaaa
     9541 accagaatca taccagcccc catgcggggg ctggtagacg atgtgatgcg cgacctgctg
     9601 acacgtatcg gcggctacga cgaatgcgtc agcgaatttg tacgcattac ccataccgtg
     9661 cattcacgat ccatatggtt aaaatatgtc cccgaaatcg ccaacggaaa caaaacgttt
     9721 tccggcacgc cctgcaccgt ccaacttctg ggcagcgatg cggacaatat ggcggcaaac
     9781 gcattggaag ccgtccgatt cggcgcggac aaaatcgatt tgaacttcgg ctgccccgcg
     9841 cccaccgtca acaaacacaa aggcggcgca attcttctga aagagccgga actgatattc
     9901 cacatcgtca aaacgctgcg cggacgtttg cctgcacata ttccgctgac ggggaaaatg
     9961 cggctaggct atgaagacaa aagccctgct ttggaatgcg cctgtgcgat tgccgaaggg
    10021 ggcgcgtgcg gactgaccgt gcacgcgcgc accaaagccg agggctacga acccccggcg
    10081 cattgggaat ggataaggaa aatccacgat accgtcaata ttcccgttac cgccaacggc
    10141 gacgttttca gcctgcagga ctatatcggc atcaaaacaa tcagcggctg caacagcgtg
    10201 atgctcggtc gcggtgcggt catccgcccc gatttggcgc gccaaatcaa gcaatacgag
    10261 aacggcggac cggtcaaaga cacggatttt gccgaagttt ccacatggat agggcagttt
    10321 ttcgagctgt gcctgacaaa agaggcaaac aacaaatacc cgatcgcccg tcttaagcaa
    10381 tggctgggca tgatgaaaaa aacgttcgac ccggcgcaaa cgctgttcga ccgcgtccga
    10441 acggttaaag atgcggatga agtgcgccga atcctgaacg catttgagca tgagatggat
    10501 gtttgatgtc tcccgcgctt caaagcaaat gccgtctgaa aacctttcag acggcatttg
    10561 ttatctttat tgccgttttt cccctgcttt cggtgtattt ttgtttttca gtttcgcccc
    10621 caaacccaaa cgtttttcat aatccgattg cggggtatcg tcttcctgca taccgaacgc
    10681 gccggcattg acccacagcg acagcagcgc gacgacaaag gcgcaaaagc cgattacata
    10741 ccaaaacatt gcccctccaa atttgttaaa atcatatcaa atacagtgcc gaatttatca
    10801 caagcgcacg agcaaataaa tcttctgacc gcagtatttc attcatttac cttgaaaaca
    10861 aaaaagggca cctgaaaatt ttgcgaaact tttcagatgc cctgctttgc cgaaaaacgt
    10921 atcagccgca gcaaccgcct tcgccgtgtc cgcctttttt gtcgttatcg acaatgggca
    10981 gttcgacttt ttcgtttggt ttttctgcat tttcgggccg gtcgttgagt ttgattttcg
    11041 cggtttcgct catttcctta cctcatttgg aaaacggttt gaaagattca taaatatagc
    11101 atgtttaatc cgaatctgaa aagggaagcc gtatgcgaaa tcccgtctga agcccaatca
    11161 gggcttcaga cgggatttgc tatctttatt gccgcttttc ttccgtatcc ggatttttgt
    11221 ttggggctga agcagattgg cagtcagatt gcaatcgaag aatgaaggca agccatcaaa
    11281 aacaaagcta tccgcttcac cgccccgata tttagaattt gtggcgcaga ccaacggagg
    11341 cggcattaat ttgagtgtag ttgccgatgc cggtatttcg tttcagccaa gcggcagaca
    11401 tgatggcgga agtgcgcttg gaaaaatcgt aatcgacacc ggcgatgatt tgatcatagc
    11461 tggtatgttc gcgtttctga ctgcgttcga caaagtcgaa accatgggca tagctgatgc
    11521 gcgggactgt attaccgaag cggtaggaag cagtggcggc aatttcggtc gtactgtttt
    11581 tggttttgtc ggcattttca gacaaatcca actgagccgc caaggcgaga ttcaagccgc
    11641 cttccccata gccgcccgtc aggcggtgta cctgatggtt tttcaatgga tcggtatcac
    11701 tcgctctgcc gagcaagaac aaaaagaaag catcatgccc ctcattggcg tgtttcgcat
    11761 atttaagggc ataatttccg aaaaagccgc catttttgta attcagaccg gcataataca
    11821 catccgaccc cggcttgccg acaacagccg gaactggttt catctgatta ctttccagcg
    11881 tgaaagtagc aggcgtatag gcggacttgc tgttttgact cggaacaaat tgaatgctgc
    11941 cgctgaaacc ggaaaatccg ggggaatcgt aacgcacgga aaccggcata ccgtcgtggc
    12001 gtttgaaaat acccaattgc gaagccacat tattattgct gtcccaagga tcaatggctt
    12061 tgctggcatc gccaaacgga ttcgcaacgc gaccggcgag cgccgtgccg aattcgcctg
    12121 ccaagccgat aaaggattcc ctgttacccc aacgggtcgc gccgccgccg gcaacggata
    12181 cgtcttgctc gagctgccaa acagccttca gcccgccgcc caaatcccca cccccttaaa
    12241 gccgataaac gagccgaaat cactgacttt cgtcctgatg cggcttttgg ccttagtaac
    12301 tgtattgccc gtttgacctt ctgagggtgg ctcggtcaac tgcagccgga tgttcctgcc
    12361 ttccacacca gctttgactt cgccgtacag gctgacatcg gcaactgccg caaacggcag
    12421 tgcggacaat acgagggcgg taagtttttt tcgcatatcg gcttcctttt gtaaatttgc
    12481 taaaaacatc gggcaaacac ccaatcagtc tccaattacc ccccgaaaaa aaaaccattt
    12541 ttcggaacaa atttcgggta aattttggaa tttttatttt gaaaaataat atttcaatat
    12601 aaaacaataa attaactttt ttgaaaatgt tgctttcccg caacatgatt taaatacaga
    12661 ccttgtcaaa gccgatgcgg cgcagacccg gatgccgtct cgttttttaa aacaccataa
    12721 aaaaacacac gcggtacttt aatttcctgc gtgtgtcgag tgtatcgaaa tcaagccgaa
    12781 tcaaatatac cggacttcga taatatcgta ttcgcgtacg ccgcccggtg cctgaacttc
    12841 cgccgtatcc ccttcttcct tgccgatcag ggcgcgggca atcggagagc cgacatagat
    12901 tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgataggtaa cgtgttcttc
    12961 cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt cggcgtggat
    13021 ttcggccgga ttgatgatgt gggcaacgga aagtttgtgt tccaactcgg aaatgcggcc
    13081 ctcgataaaa ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg acaaatcgcc
    13141 gtgcgatctg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc ttttgagctg
    13201 ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt tttgcatcgg
    13261 tctttttctc catattccgg cacaccggtt tgcggcggca agcataccgc gtaccgtctt
    13321 gttttgtgcg tccggatatt aaaataaaaa tacaagccgc ccggaaaatc ggcggcttgt
    13381 ctgtcgttga acagcggcta ttctaccaaa ttctatgaaa ttggcaatcg tgccgtgccg
    13441 ccggcagacg cgctatgtcc gcaacaaaag ccgaaaatat gccggcaaaa aaattttaga
    13501 aacaaaaaat ttaaaaaaaa tcaattttcg gcataaaaaa ccaaattcac gtactttaaa
    13561 accgaaaatg ccaaacctga gattttccag acggcatttg caccagtata atgcaggctg
    13621 tttttatctt tatatttacg ttttgccatg accgaatccg tccgcctccc cgccgcctcg
    13681 ctcaaacctt ccaccgtcgc cctgcccggc tccaaaagca tcagcaaccg caccctgctg
    13741 ctcgccgccc tgtccgacaa tgtttgcgaa atccattccc tgctcaaatc cgacgatacc
    13801 gaccgtatgc tcgaagcact cgataaactc ggcgttcaaa tcgaacatct tgccgaaggc
    13861 cgtctgaaag tacacggcac gggcggacgc ttccccaacc gctctgccga tttgtttttg
    13921 ggcaacgcgg gcacggcgtt ccgtccgctg accgccgcgc tggccgtttt gggcggcgat
    13981 tatcatctgc acggcgtgcc tcgtatgcac gaacgcccca tcggcgattt ggtcgatgcg
    14041 ttgcggattg ccggcgccga tgtcgaatat ctcggcaacg aacactatcc gccgcttcat
    14101 atcggcaaac gtcaagactg cggcgagcgc gtgattccta ttaaaggcaa tgtgtccagc
    14161 cagtttctga ccgccctttt gatggcgttg ccgctgaccg ggcaggcgtt tgaaatccgt
    14221 atggtcggcg agttgatttc caagccctat atcgacatta ctttgaaact gatggcgcaa
    14281 ttcggcgtac aggttgccaa tgaaggctac cgcgttttca aaatccctgc cgatgcgcac
    14341 tatcacgcac ccgaacactt gcacgtcgaa ggcgatgcct ccggcgcgtc ctacttcctc
    14401 gcagccggtt tgattgccgc cacgcccgtc cgcgttaccg gcatcggcgc aaacagcata
    14461 cagggcgatg tcgcctttgc ccgcgaactg gaaaaaatcg gtgcggacgt ggtttggggc
    14521 gaaaacttcg tcgaagtctc gcgcccgaaa ggacgcgccg tccaagcctt tgatttggat
    14581 gcgaaccata tccccgatgc cgcgatgacc ctcgccatcg tcgcgcttgc cacaaggcaa
    14641 acctgcacgc ttcgcaacat cggttcgtgg cgcgttaagg aaaccgaccg catcgccgca
    14701 atggcgaacg agttgcgcaa actcggtgca aaagtcgtcg aagaagccga agcgattcac
    14761 attaccccgc ccgaaacgcc gacacccgat gccgtcatcg acacttacga cgaccaccgc
    14821 atggcgatgt gtttctcgct gatttcgctg ttgggagtac ccgtcgtcat caacgacccg
    14881 aaatgcaccc ataaaacctt cccgacttat tttgaagtgt tctcatcact gaccgaaaca
    14941 gcggaataag gcggcaaaat gccgtctgaa acccgaaaac agggcttcag acggcatttt
    15001 gcc
//
LOCUS       DAWTCI010000051        14721 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWTCI010000051 DAWTCI010000000
VERSION     DAWTCI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14721
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.051"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..556
                     /locus_tag="WM481_001471"
     CDS             <1..556
                     /locus_tag="WM481_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFA9080768.1"
                     /translation="TAESVAALLAAALCLTGIFSPLTSLLLPENYAAVRFTVVSCMLP
                     PLFCTLAEISGIGLNVVRKTRPIAFATLGALAANLLLLGLSVPAGGARGAAVACAASF
                     WLFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLA
                     GCILRHRKNLHKLFHYLKKQGFPL"
     gene            553..1621
                     /locus_tag="WM481_001472"
                     /pseudo
     CDS             553..1621
                     /locus_tag="WM481_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            1635..2807
                     /locus_tag="WM481_001473"
     CDS             1635..2807
                     /locus_tag="WM481_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFA9080769.1"
                     /translation="MNITIAAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVILLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVIYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDIAVNAIHSYAMQSITNKLSDYMALQKPILNSQVHDEVAEVLTLL
                     PHENYRSGDVDGFVQAAKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLADE"
     gene            2800..4041
                     /locus_tag="WM481_001474"
     CDS             2800..4041
                     /locus_tag="WM481_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFA9080770.1"
                     /translation="MNKFFKRLIDIIASASGLIVLSPVFLVLIYLIRKNLGSPVFFIR
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDSERLTPFGKKLRTASLDELPELWNVLK
                     GEMSLVGLRPLLMQYLPLYNKFQNRRHEMKPGITGWAQVNGRNALSWDEKFSCDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAQGEATMPPFAGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVIGTTLLLENSLSPEQFDITVAVGNNRIRRQITE
                     KAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDG
                     MTVAGNPAKPLTGKNPKTGTA"
     gene            4172..5347
                     /locus_tag="WM481_001475"
     CDS             4172..5347
                     /locus_tag="WM481_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HFA9080771.1"
                     /translation="MLNTALSPWPSFTREEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALSNGTLALDAALKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAVIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGATYKGKSVGSIGHVGAWSFCQDKIITTGGEGGMVTTNDKTLWEKMWAYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKHLPEWTARRQENAAKLAESLR
                     KFKSIRLIEVAGYIGHAQYKFYAFVKPEHLKDDWTRDRIVSELNARNVPCYQGGCSEV
                     YLEKAFDNTPWRPKERLKNAVELGGTALTFLVHPTLTDDEIAFCKKHIEAVLTEAAR"
     gene            5395..7305
                     /locus_tag="WM481_001476"
     CDS             5395..7305
                     /locus_tag="WM481_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFA9080772.1"
                     /translation="MNLETLIALPRNIKKICFLIHDFLMIFTAFWFTQSLKADYSDEW
                     FDFANWQSFLLTALLTITLFIRMGLYRAVTRFVSFHVLTTAFAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSDHQKKTHDPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDNPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPSATQEQ
                     RRRIISKLKAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     TEGKTVMVTGAGGSIGSELCRQIIRRRPERLLLFELSEFALYTVEKELCEYCARNGIA
                     AEILPFLGSVQNRTLLTHIMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVETFVLISTDKAVRPANTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPITLTHPDITRYFMTIPEAAQLVIQAGAMGRGGDVFVL
                     DMGESVRITDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADETMLPWHELSALLDRIRTACDRYDQQAIRDLLVNAPTGFTPTGGICDL
                     LWVCETHRKNAV"
     gene            7361..8653
                     /locus_tag="WM481_001477"
     CDS             7361..8653
                     /locus_tag="WM481_001477"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFA9080773.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFPNREYGWNLTVGNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGRHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPPIIDNAYGMPFPNIIDSGVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEAVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKPYDNV"
     gene            complement(8705..9541)
                     /locus_tag="WM481_001478"
     CDS             complement(8705..9541)
                     /locus_tag="WM481_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFA9080774.1"
                     /translation="MIDLHCHSTVSDGMLPPAEVVRLAHQNGCTLLALTDHDHTGGIS
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLADVRKGRLKRLE
                     AIAAKLEKKGIGGAYDGALALAANKEMVSRTHVAEFLIKNGHVKNKRQAFTKYLGDGK
                     SCAVRHEWATLADCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLADRFGLLASAGSDFHRLDDFSGGILGACPDLPENCKPVRAHFS
                     RH"
     gene            complement(9836..10039)
                     /locus_tag="WM481_001479"
     CDS             complement(9836..10039)
                     /locus_tag="WM481_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080775.1"
                     /translation="MDTTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRKKLKEAGWPDDAV"
     gene            complement(10017..10267)
                     /locus_tag="WM481_001480"
                     /pseudo
     CDS             complement(10017..10267)
                     /locus_tag="WM481_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
     gene            complement(10279..10476)
                     /locus_tag="WM481_001481"
     CDS             complement(10279..10476)
                     /locus_tag="WM481_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080776.1"
                     /translation="MCISVNADSSGNRICSLPLGRISLDGWRHTGFQTAFLRNRQCGH
                     IAGFAAILGAAAAGATITPSF"
     gene            10800..14234
                     /gene="dnaE"
                     /locus_tag="WM481_001482"
     CDS             10800..14234
                     /gene="dnaE"
                     /locus_tag="WM481_001482"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFA9080777.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPVGAADVWIGNPNAPDKPFRAMLVIRNDAGYLRLSELLT
                     EAYVGQDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDTFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDCLIKLSNEGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLDDAMKAQPQIQELLEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTAGDIVDVGKIPLDDQTAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPTKLGR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDSSGQAEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
BASE COUNT         3453 a         4494 c         3786 g         2988 t
ORIGIN      
        1 aacggcagaa tctgtcgccg ccctgcttgc cgccgccctc tgcctgaccg gcattttctc
       61 gcccctcacc tccctcctac tgccggaaaa ctacgccgcc gtccggttta ccgtcgtatc
      121 gtgtatgctg ccgccgctgt tttgcacgct ggcagaaatc agcggcatcg gtttgaacgt
      181 cgtccgcaaa acgcgtccga ttgcattcgc caccttgggc gcgctggcgg caaacctgct
      241 gctgctgggg ctttccgtac cggccggcgg ggcgcggggc gcggcggttg cctgtgccgc
      301 ttcattctgg ctgttttttg ttttcaagac cgaaagctcc tgccgcctgt ggcagccgct
      361 caaacgcctg ccgctttata tgcacacatt gttctgcctg gcctcctcgg cggcttacac
      421 ctgcttcggc acgccggcaa actaccccct gtttgccggc gtatgggcgg catatctggc
      481 aggctgcatc ctgcgccacc ggaaaaattt gcacaaactg tttcattatt tgaaaaaaca
      541 aggtttccca ttatgaaaat catcctgact acttctatgt cgggtctggg cggcacagaa
      601 accgccaccg tccgcctcgg gcggctgctc aaacggcacg ggcacgacat catcctcgcc
      661 tcctcggacg gcccttttgt cggagaggcg caagcatcgg gcatccgttg gcagccggtt
      721 gatttttacc ggggcgggct tgccggctac cttaaaagca cctttgccta cgcccggatg
      781 ctgcgccgcg aacaacccga catcatcgac tgccaaatgg cgcgcgtcgt cccggcctgc
      841 gcccttgccg ccaaaatcgt ttcaccgaaa accaaaatca tctgccactc gcacggtttg
      901 gacgcggcaa cctaccccaa aaccgccaaa ctcttcgaca agctgggcgc gtacatcatc
      961 ggcaactgca aacacgaacg cgaaaagctc atccgccacg gcttccccgc cggccggatt
     1021 gcctacgcct acaacacccc cccccccgga atttcatttc cggaaaaccg aaaaagaatg
     1081 cgccgtactc ggcacacttt cccgtttgga caccgtccgc gccgtgcatc tgatgttgga
     1141 tattttgaag aaaatggttg gccgcaatat acccgtacgc ctgaacatgg cgggcatagg
     1201 agaagaaatg gacaacctca aagcccaagc caaacgtttg ggtattgaag acaaagtgac
     1261 cttcctcggc ggcgtccgcg atttgaccgg ctacttcaaa gaagtcgata ttttagtgaa
     1321 cacgccgcat tgcgtaggtg accacggcgc aggtgtcggc aacaatattt tggaagccgg
     1381 cctttacgac acgcctgtcg tgacctacaa catggcgggc atttccgaaa tggtcattac
     1441 cggccaaacc ggctactgca ttcctttcgg cgatgacgaa gcgtttatcg aagccgtcga
     1501 tacactcatc aagcatccag agttgcgcag ccaaatgggc aaagccctgc acaaacatgt
     1561 cgaaacctta tgctccgacg acgaaatcta ccgcaccacc atggctgcgt acgaaatgta
     1621 aggccgtctg aaacatgaac attaccatag ccgcccccta ctgctcgctg ccgtccgaac
     1681 cgcatttcaa ccgcttttgg tatctggcag aactattgtc gcaatcgcac gacgtgttgc
     1741 tgattaccag caacttcaag cactacgaca aatctttcag acggcccgaa gatgccgagg
     1801 ccgcctcaca aggccgtctg aaagtcatac tgttggaaga aagcggatac agcaaaaacg
     1861 tatctttggg acgcgtgacc agccatcacc gcttcgtcaa acattttgaa aaatggttgg
     1921 aaaactgccg tccgggcgaa caagacgtca tctattccgc ctatccgctg attgccacca
     1981 acctgctgtt gggcaaacac aaagcgcgtt tgggttacaa actgattgtc gatgtgcagg
     2041 acgtatggcc ggagtctttc tcctcggtcg taccgttttt gaaaaaagta ccgcacaagc
     2101 tgctgccctt tgcttcacgc gccaaccggg cctaccgcta cgccgacgcg ctggttgccg
     2161 tatcgcagac ttatctcgac cgcgccaaag aaaccaatcc gaacgtaccc ggcgaagtca
     2221 tctatattgg tgctgacttt cccaaactcg atgccgcacc tgccaaagat tttggcgacg
     2281 acaaaacccg ctttttctac ttgggcacgc tcagttacag ctatgacgtg gaaaccgtgt
     2341 gcaaaggcgt tcggaaactt ttggacgacg gcgaaaatgt agagctgcac atcatgggcg
     2401 gcggccccga tttggacagg ctcaaacaat atgcctgcga cggcatcaag ttttacggct
     2461 acattcctta cgccgaaatg atgtcggtcg ccaagggctg cgacattgcc gtcaacgcca
     2521 tccactctta cgccatgcag tcgattacca acaaactctc cgactatatg gctttgcaaa
     2581 aaccgattct gaacagccag gtccacgacg aagttgccga agtccttacc ctgctgccgc
     2641 acgaaaacta ccgttccggc gatgtggacg gtttcgtcca agccgccaaa gatattttga
     2701 agcgcaaaaa cgatcctgtt cagtccgaag aaatcgtccg ccgtttcaag cgcgacgttg
     2761 cctatcaaaa aatcgtcaac ctgattgaaa gattagctga tgaataaatt tttcaaacgc
     2821 ctcatcgaca tcatcgcatc cgcatcgggg ctgattgtcc tgtcgcccgt gtttctggtt
     2881 ttgatatacc tcatccgcaa gaatctgggt tcgcccgtct tcttcattcg ggaacgcccc
     2941 ggaaaggacg gaaaaccttt taaaatggtc aaattccgtt ccatgcgcga cgcgcttgat
     3001 tcagacggca ttccgctgcc cgacagcgag cggctcacgc ccttcggcaa aaaactgcgc
     3061 accgccagtt tggacgaact gcctgaactg tggaacgtcc tcaaaggcga gatgagcctg
     3121 gtcggcctcc gcccgctttt gatgcagtat ctgccgcttt acaacaaatt tcaaaaccgc
     3181 cgccacgaaa tgaaaccggg cattaccggc tgggcgcagg tcaacgggcg caacgcgctt
     3241 tcgtgggacg aaaagttctc ctgcgatgtt tggtacaccg acaatttcag cttttggctg
     3301 gatatgaaaa tcctgtttct gacagtcaaa aaagtcttga ttaaagaagg catttcggcg
     3361 caaggggaag ccaccatgcc ccctttcgcg gggaatcgca aactcgccgt tatcggcgcg
     3421 ggcggacacg gcaaagtcgt tgccgagctt gccgccgcac tcggcacata cggcgaaatc
     3481 gtttttctgg acgaccgcac ccaaggcagc gtcaacggct tccccgtcat cggcacgacg
     3541 ctgctgcttg aaaacagttt atcgcccgaa caattcgaca tcaccgtcgc cgtcggcaac
     3601 aaccgcatcc gccgccaaat caccgaaaaa gccgccgcgc tcggcttcaa actgcccgtt
     3661 ctgattcatc ccgacgcgac cgtctcgcct tctgcaataa tcggacaagg cagcgtcgta
     3721 atggcgaaag ccgtcgtaca ggccggcagc gtattgaaag acggcgtgat tgtgaacact
     3781 gccgccaccg tcgatcacga ctgcctgctt gacgctttcg tccacatcag cccgggcgcg
     3841 cacctgtcag gcaacacgcg tatcggcgaa gaaagccgga taggcacggg cgcgtgcagc
     3901 cgccagcaga caaccgtcgg cagcggggtt accgccggtg caggggcggt tatcgtatgc
     3961 gacatcccgg acggcatgac cgtcgcgggc aacccggcaa agccccttac gggcaaaaac
     4021 cccaagaccg ggacggcata agcgattaaa ataaacttcc gtacaaaccg attttgacaa
     4081 cacctgcggc gcgcgcccga ttcttcggaa cacgccccct tcagacggca tagggtcgga
     4141 aatgccgtcc gaacccgacg gaccaaacac catgctgaac accgcccttt ccccatggcc
     4201 gagcttcacc cgagaagaag ccgatgccgt ttccaaagtc ctgctgtcca acaaagtcaa
     4261 ctactggacg ggcagcgaat gccgcgaatt tgaaaaagaa tttgccgcct ttgccggcac
     4321 gcggtacgcc gtcgcccttt ccaacggcac gcttgcgctc gatgccgcac tcaaagcaat
     4381 cggcataggc gcgggcgacg atgtgattgt tacctcgcgc accttcctcg cctccgcctc
     4441 ctgcattgtg aacgcgggcg caaaccccgt gtttgccgac gtggatttga acagccaaaa
     4501 catcagcgcg gaaaccgtca aagccgtgct gaccccgaat accaaagccg tcattgtcgt
     4561 ccaccttgcc ggtatgcctg ccgaaatgga cggcatcatg gctttggcaa aagaacatga
     4621 tttgtgggtg attgaagact gcgcccaagc gcacggcgcg acctacaaag gcaaatccgt
     4681 cggctctatc gggcacgtcg gcgcgtggtc gttctgtcaa gacaaaatca tcaccaccgg
     4741 cggcgagggc ggtatggtta cgaccaacga caaaaccctg tgggaaaaaa tgtgggcgta
     4801 caaagaccac ggcaaaagct acgatgccgt gtaccaccgc gaacacgcgc ccggtttccg
     4861 ctggctgcac gaaagtttcg gcaccaactg gcgcatgatg gaaatgcagg ctgtcatcgg
     4921 ccgcatccag ctcaaacacc tgcccgaatg gacggcgcgc cgccaagaaa acgccgccaa
     4981 gctggcagaa agtttgcgca aattcaaaag catccgcctg attgaagtcg ccggctacat
     5041 cggacacgcg caatacaagt tctacgcctt cgtcaaaccc gaacacctga aagacgactg
     5101 gacgcgcgac cgcatcgtca gcgaactgaa cgcgcgcaac gtcccctgct atcaaggcgg
     5161 ctgctccgaa gtgtatttgg aaaaagcctt cgacaacacg ccgtggcggc ccaaagagcg
     5221 gctgaaaaac gccgtcgaac tgggcggcac cgccctgacg ttcttggtgc acccgacgct
     5281 gaccgacgac gaaatcgcgt tctgcaaaaa acacatcgaa gccgtcctga ccgaagccgc
     5341 acgataaccc ttcagacggc atatgccgcc tgaaagcaca taccgcccca cgatatgaat
     5401 ctggaaaccc tgatcgccct gccgcgcaac atcaagaaaa tctgtttcct catacacgat
     5461 tttctgatga ttttcaccgc cttttggttc acccaaagcc tgaaggccga ctactcggac
     5521 gaatggttcg attttgccaa ctggcagtct tttttgctga ctgccttgct gaccatcaca
     5581 ttatttatcc gaatggggct ttaccgcgcc gttacacgct ttgtcagttt ccacgtcctg
     5641 accaccgcct ttgccggcag cctcgcctcc gccgtattgt tcttcctcaa cacattgatt
     5701 tttgaagaaa ggctgcgcct cgccctgccg attgtctatt tcttactgct gtttgtttcc
     5761 gtgaccggct cgcgtatggt tttgcgcgga ctcctgtccg accaccagaa aaaaacacat
     5821 gaccccgtca tcatttacgg cgcggggcgt tcgggtcggc agctccttga agccgtcaaa
     5881 cagatgcgcg aatattccgc cgccgccttt gtggacgaca accccaagct ttggcacacc
     5941 gtcatctacg accttgccgt ttaccagccc gatgccatcg ccttcctcat cgaacgctac
     6001 ggcgtggaaa aaatcctgct cgccattccc agcgcgactc aggaacaacg ccgccgaatc
     6061 atcagcaaac tgaaagccta tccgtgcgaa gtgttgacca ttcccggaat gaaagacctg
     6121 atggacggga aaatcagcat cggcacgctc aaaaaaatct ctgtgtccga cctgctcggg
     6181 cgcgattccg tcgcacccga cgaccgcctc atgaatgccg acaccgaagg caaaaccgtc
     6241 atggtaaccg gcgcgggcgg ctccatcggt tcggaactct gccgccagat tatccgccgc
     6301 cgccccgaaa ggctgctgct gttcgagctg tccgaattcg ccctgtacac cgttgaaaaa
     6361 gaattgtgcg aatactgcgc ccgaaacggc atcgccgccg aaatccttcc ctttttgggt
     6421 tcggtgcaaa accgcacgct gctcacgcac attatgaccg ccttttccgt tgcgaccgtc
     6481 tatcacgccg ccgcctacaa acacgtccct atggtcgagt tcaacaccgt cgagggcata
     6541 cgcaacaaca tcttcggcac actcgaatgc gcgcttgccg ccacggcttc gggcgtggaa
     6601 accttcgtcc tcatctccac cgacaaagcc gtccgccccg ccaacacgat gggtgcgggc
     6661 aaacgcatgg cggaactctg ccttcaggca ctcgccgccg aacccggaca aaaaacccgc
     6721 ttcagcatgg tacgtttcgg caatgtttta ggctcgtccg gctccgtcgt cccgctgttt
     6781 gaaaaacaga ttgccgaagg cggccccatc accctgaccc accccgacat cacgcgctat
     6841 ttcatgacca tacccgaagc ggcgcaactg gttatccaag ccggcgcgat gggcagaggc
     6901 ggcgacgtgt tcgtcctcga tatgggcgaa tccgtcagaa tcaccgacct tgcccgccaa
     6961 atgattacct taagcggact caaacccaaa acgcccgaac agcccgacgg cgacatcgaa
     7021 atcctcatta ccgggctgcg ccccggcgaa aaactctacg aagagttgct catcggcgac
     7081 aacgtccgca aaaccggcca tccgcgcatc atgaccgccg acgagaccat gctgccgtgg
     7141 cacgaactct ccgccctgct cgaccgcatc cgcaccgcct gcgaccgtta cgaccagcag
     7201 gcaatccgcg acctgctcgt caacgccccg accggcttta ccccgaccgg cggcatctgc
     7261 gacctgcttt gggtatgcga aacacacaga aaaaatgccg tctgaacctt cagacggcat
     7321 accgtacaaa ccaatctgcc ttacacacac tgagtttgac atgcagttct cggcattcgg
     7381 cgaaaaattc acgcaacaca gcggcatcct gcaactgatg gacgacctcg gcgacgcgct
     7441 caaaagcgac aagcccgtca atatgctcgg cggcggcaac ccggcgcgca ttcccgaaat
     7501 cgatcgggcg ttcgccgaca tattttccaa gctggcggcg gaacacgccg tcgagaacat
     7561 cggcaactac tccaatcccc aaggcgatgc cgcgctgatt gacgcgctga ccgccttccc
     7621 caaccgcgaa tacggctgga acctgaccgt cggcaatatc gcgctgacca acggttcgca
     7681 aaacgcgttt ttctatttat tcaacctctt cggcggcaaa ttcaagcttt cagacggcac
     7741 atccgcagaa aaagccattt tgttgccgct cgcgcccgaa tacatcggct atgccgacgt
     7801 gcatgtcgaa gggcggcact tcgtttccgt caaacccaaa atcgaaaacg tcgaacacga
     7861 aggcgaagca ggcttcttca aataccgcgt ggactttgac gcactggaaa acctgcccga
     7921 actcaaagcg ggcaaaatcg gcgcgatttg ctgttcgcgc ccgaccaacc cgaccggcaa
     7981 cgtgttgacc gacggcgaaa tggcgcgttt ggacgctttg gcgcgtgaac acggcattcc
     8041 gccgattatc gacaacgcct acggaatgcc gttccccaac attattgaca gcggcgtaac
     8101 gctgaattgg cacgaaaaca tcatcctctg cttcagcctt tccaaagtcg gcctgccggg
     8161 cgttcgcacc ggcatcatcg tcgccgcgcc cgaagccgtc aaagccgtca gcagcctgaa
     8221 cgcgattgtg aaccttgccc ccacgcgctt cggcgcggcc atcgccgccc cgctgctgga
     8281 aagcggcgag atgaaacggc ttgccgacca agtcatccgg ccgttttacc gcaatcaggc
     8341 gcaaaccgcc gtttcgctgc tcaagcgcga gctgggcgcg tacccgatga aaatccacaa
     8401 acccgaaggc gcgattttcc tgtggctctg gtttgaaaac ctgcccgttt cgtcgcaaac
     8461 cctgtacgaa atgctcaaag ccgaaggtac gctgattatt ccgggcgaac atttcttcgt
     8521 cggcatcgac acgcaagact acccgcacgc gggcgagtgc atccgtatga gtatcgcaca
     8581 ggacgctcaa acgctggaaa aaggcatcgc cgccatcggc aaaaccgtcc gaaaaccgta
     8641 cgacaacgtt taaaacgcaa aaaatgccgt ctgaagacct ttcagacggc attttcatcc
     8701 gcattcaatg tcgggaaaaa tgcgcccgca ccggtttgca gttttccgga agatcgggac
     8761 acgcgccgag gatgccgccg ctgaagtcgt ctaaacggtg gaagtcgctg cccgcgcttg
     8821 ccaacaatcc gaagcggtct gccaaaagcg cgtagttgag gcggtcgttt ttgcagcagt
     8881 tgccgctgtg gacttcgatg cccgcgccgc cgaggttttt aaattcttca aacaggttgc
     8941 gcttggcggt tgccgacaaa tcataacgca tcggatgggc aatcaccgcc atgccgcccg
     9001 cgccgttgac ggcggagacg cagtctgcca gcgtcgccca ttcgtggcgt accgcgcagg
     9061 atttgccgtc gcccaagtat ttggtgaacg cctgccgctt gtttttgacg tgtccgtttt
     9121 tgatgaggaa ctcggcgacg tgggtgcggc tgaccatttc tttgtttgcc gccagcgcca
     9181 gcgcgccgtc gtatgcgccg ccgatgcctt tcttttcgag cttggcggcg atggcttcaa
     9241 gacgtttcag acggcctttg cggacatctg ccaacaggtt ttgcaggttt tcgtcctgct
     9301 cgtcgaaatc caaaccgaca acgtgtatgg tacgcccgcg ccacgttacc gaaatttcca
     9361 cgccgttaat caggcgcaaa ccgagcctgt cggcttcggc acgcgcttcg gagatgccgc
     9421 ccgtgtggtc gtggtcggtc aacgccagca gcgtgcagcc gttttgatgt gcgaggcgga
     9481 cgacttcggc gggggggagc ataccgtcgg aaacggtgga atggcagtgc aggtctatca
     9541 tggggtgtta tgtggtggtg aatgaaggac gggatgtttc aatgttgtcg gacagtgtgg
     9601 cggcggcggt cattttaaaa aacaggtgta tggcaaatgc aaaggaaaag ccgctatgcc
     9661 gtcattcccg cgcaggcgag aatccagact ttgatttatt ggaaatgttt aaggttaacc
     9721 gctatttcaa acttccggat tcctgctttc gagggaatgg cgatatgaac gttttcaatt
     9781 ttaatctact ataaaagacc gtctgaaaac gtggttttat caagcccaac cgttttcaga
     9841 cggcatcgtc cggccaaccc gcttccttca gttttttccg ttgcgcatcg tagcgtgcct
     9901 tgtccgccca gtaaatcgtc tgaatgcacg cctcgccgca gtcgctgccg cagcattccc
     9961 acgactcggg gcggacgggt tcgtccaaaa gcggctcgcc caaaagggct tcggctttat
    10021 gcttcagggt cgtatccatc ggcgatttcc aagcgcgcgc cgtcaaactc gatgacttct
    10081 ccgccgcgta ttttggccgt tttggtattt tggccgtttt gcggatttcg gttccgccgt
    10141 tgcgcaacac cagcccttcg gcgataaatg cttttgcctg tccgccgctt tcggcaagtc
    10201 cggccaattt caagaggtcg cacaaagcga tgtattcgtt gtcttcgaga tagacggtgg
    10261 cttccataat gtcccctttt agaaagaggg cgttattgta gcacctgccg ccgccgcacc
    10321 caaaattgcc gcaaaaccgg cgatgtgtcc gcactgcctg ttccgtaaaa atgccgtctg
    10381 aaaccccgta tgccgccatc cgtccaaaga aatcctgccc aacggcaggc tgcaaatcct
    10441 gttccccgac gaatccgcat tgacgctgat gcacatcctc aaacgcggac tgcccgatac
    10501 accggcaatc ggcatcaaaa cgaaatcaaa aacctgtttg aacgtaaaat ctatttaact
    10561 gcttgccgtc cgtccgaata aggtatatag ttccttataa ctagtttgat agtcctttat
    10621 atctatcaat actccttggg aagcctccgc cacacggcag gaggcatttt tttgccgtcg
    10681 taaaagctca aaaaacattt gcaggtcatg ccgtctgaat ccgaaacggc attacctgca
    10741 ccgccatcta aagacaaccc tgctacaata cgccttttat tgtccacgcc gattttgcca
    10801 tgaccgagcc gacctacatc cccctgcgcc tgcataccga attctccatt accgacggta
    10861 tggtgcggat taaaaaactg attgccaaag cgcaagaata cggtttgcct gctttgggca
    10921 tcagcgattt gatgaacgaa ttcggtttgg tgaaattcta taaagcctgc cgcggcgcag
    10981 ggattaagcc cgtcggcgcg gcggacgtgt ggataggcaa tccgaatgcg cccgacaagc
    11041 cgttccgcgc catgctggtt atccgcaacg atgcgggtta tctgcgcttg agcgagcttt
    11101 tgacggaggc ttatgtcggc caagaccgca atatccatca tgcggaactc aatcccgaat
    11161 ggctggaaaa cggcgacaac agcggcttga tttgtttgag cggcgcgcat tacggcgaag
    11221 tgggcgtgaa tctgttgaac ggcaatgaag acgcggcgcg tgcggcggcg ttgaaatatg
    11281 cggcatggtt tcccgatacg ttttatttag agctgcaacg cctgcccgaa cgccccgaat
    11341 gggaggcttg cgtttccggc agcgtgaagc tggcggagga actgggtttg ccggtggtag
    11401 caacgcatcc gacacagttt atgaaccgcg acgatttcaa cgcgcacgag gcgcgggtgt
    11461 gcatcgcggg cggctgggtg ctgacggaca agaaacgtcc gcgcgatttc acgccgagcc
    11521 agtttttcat tccgccggaa acgatgctgg agcgtttcgc cgacttgccc gaagccttgg
    11581 aaaacacggt ggaaatcgcc aaacgctgca acttgcacat cacattgggc aaaaacttcc
    11641 tacccctttt ccccacgccc gacggtttat cactcgatga ctgtctcatc aaactttcca
    11701 acgagggttt gcaggaacgc atggttcagc tttatcccga cgaggcggaa cgggcggcaa
    11761 aaatgccgga atatcaggag cggctggact ttgagctgaa catcatcatc caaatgaaat
    11821 tccccggcta tttccttatc gtacaagact ttatcaactg ggcgaaaacg cacggctgtc
    11881 cggtcgggcc gggacgcggt tcgggcgcgg gttcgctggt ggcgtattcg ctgaagatta
    11941 ccgacctcga cccgctgaaa tacgcgctgc tgttcgagcg tttcctaaac cccgaacgcg
    12001 tttccatgcc cgactttgac gtggacttct gtcaggcaaa ccgcggccgc gtgattgaat
    12061 acgtgcgcga aaaatacggc gctcaggcgg tcagccagat tgtgactttc ggcacgatgt
    12121 cgtccaaagc ggtcatccgc gacgtggggc gtgtgctgga actgccgttt atgctgtgcg
    12181 acaaactgtc caagctgatt ccgttggaag ccaacaaacc cctgagtttg gacgacgcga
    12241 tgaaggcgca gccgcagatt caggaattgc ttgaagcgga agaagcggac gaactgatta
    12301 cgctggcgaa aaaactggaa gacctgacgc gcggtttggg tatgcacgca ggcggcgtgt
    12361 tgattgcgcc gggcaagatt tccgattaca gccccgtgta tcaggcggac gaatccgcct
    12421 cgcccgtatc catgtacgac aagggcgacg tggaagacgt gggtttggtg aagttcgact
    12481 ttttgggcct gcgcaacctg accattatcg aaatggcgca gaacaacatc aaaaacaccg
    12541 ccggcgatat cgtcgatgtc ggcaagattc cgcttgacga ccagaccgcc taccaaatct
    12601 tccgcgatgc gaacaccacc gccgtcttcc agttcgagtc gaccggcatg aaaaaaatgc
    12661 tgaaaacggc gcatacgacc aagtttgaag aactcatcgc cttcgtatcg ctctaccgac
    12721 ccggcccgat ggacaacatt cccgacttcg tcgcacgcat gaagggacag gaattccaat
    12781 acatccaccc gctgctggaa ggcatcctcg cgccgaccta cggcattatg gtgtatcagg
    12841 aacaagtgat gcaggcggcg cagattatcg gcggctactc gctcggcggc gcggacctgc
    12901 tgcgccgcgc catgggtaag aaaaagcccg aagagatggt gaaacaccgc gaaatcttcg
    12961 ccgaaggtgc ggcaaaacaa ggcatttcgc gcgaaaaatc cgacgaaatc ttcaactaca
    13021 tggaaaaatt cgccggctac ggtttcaaca aatcccacgc cgccgcctac gccctgattt
    13081 cctaccagac cgcatggctc aaagcccact atcccgccga atttatggcg gcgacgatgt
    13141 cgtccgaatt ggacaacacc gaccagctca aacatttcta cgacgactgc cgcgccaacg
    13201 gcatcgagtt cctgccgccc gacattaacg aatccgacta ccgcttcacg ccgtatccga
    13261 acatgaaaat ccgctacgca ctcggcgcga ttaaaggcac gggcgaagcc gccgtcgaat
    13321 ccatcatcgc cgcgcggcaa agcggcggca aatttaccgg cctgttggac ttctgcgagc
    13381 gcgtcggcaa agaacacatg aaccgccgca ccctcgaagc cctgatacgc ggcggcgcgt
    13441 tcgacagcat cgaacccaac cgcgccatgc tcttggcgaa catcgacctt gccatggaca
    13501 acgccgacca aaaagccgcc aacgccaatc agggcgggct gtttgacatg atggaagacg
    13561 ccatcgaacc ggtgcggctc atcgacgcac ccatgtggag cgaatcggaa aaactcgccg
    13621 aagaaaaaac cgtcatcggc ttttacctgt ccggccaccc gttcggcccg tatgcccaag
    13681 aagtccgcca aatcgccccg accaaattag gccgtctgaa accgcaagac agcgtgcgcc
    13741 tcgccggatt cgttaccgcc gtgcgcacca tgatgggcaa acgcggcaaa atcgccttcg
    13801 tcagcctcga agattcgagc gggcaggctg aaatcatggt cggcggacaa acgctggaaa
    13861 actgcgccga ctgcctcaaa gccgaccaag tgctgattat cgaatccaaa gtcagccgcg
    13921 acgattacgg cggcggcgac gggctgcgca tcatggcaaa ccaagtcatg accctgcaaa
    13981 cggcgcgcga acgctacgcc cgcagcctca gcctcgccct tgccccgcat cacgacatcg
    14041 gcggactggt acggctgctc gccgcccacc aactgcccga cacgccgcgc atcccgcttc
    14101 aattgtcgta tgccaacgaa aaagcgtcgg gcaggcttca agtgcctccg aaatggacgg
    14161 ttacaccgag ttccgcattg ttcggcgaac tggaaacatt gctcggcagc cggtcggtgc
    14221 gcgtgaattg gtaaccaaaa atacaaatgc cgtctgaagc ccaaaaccgg tttcattcgt
    14281 actttattcg aatgattgaa taaaagtaac tgccaagaaa aacgtatttt ttggttattt
    14341 cgccagccta aataaatcaa ccggggcgat tgatatccgt gtacatgaca cagacagcac
    14401 caaagggaaa aacggcattt tcaaaagtat cggtgtcaaa accgcccttt cattccaaaa
    14461 ataccaaatc gacgaaccgg gcaaagaaat cagaccgtgc cgcctgaaaa aacgcccgcc
    14521 cgtccgttaa attaatctat ccctgtttca gacggcctga aacagggatt tttatatcaa
    14581 gataaaatga gaacgggagc gacaaccacg ggcaaagtcg gcgcgaacta tcaaaaattg
    14641 tagcactgcg aaatgaggac gggagcaata acccttaggt agctcttgtt atagtggatt
    14701 aacgaaaatc aggacaaggc g
//
LOCUS       DAWTCI010000052        14697 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWTCI010000052 DAWTCI010000000
VERSION     DAWTCI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14697
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.052"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(321..4490)
                     /locus_tag="WM481_001483"
     CDS             complement(321..4490)
                     /locus_tag="WM481_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HFA9080778.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPAPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPA
                     TVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KADFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGTGTARTDGSLAIASDPAN
                     EQRKLVFDTVNISAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGNING
                     SIHLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGTARN
                     LHIGKAADIRSLDFTLKGSPDTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQH
                     RIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQ
                     IGGRVSSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAASLPVNMNLTLDLN
                     DGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSD
                     KKDSAGNGKGK"
     gene            complement(4545..6392)
                     /locus_tag="WM481_001484"
     CDS             complement(4545..6392)
                     /locus_tag="WM481_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HFA9080779.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     SKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYQGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6764..7993
                     /gene="sstT"
                     /locus_tag="WM481_001485"
     CDS             6764..7993
                     /gene="sstT"
                     /locus_tag="WM481_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFA9080780.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALAVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8144..9424
                     /locus_tag="WM481_001486"
     CDS             8144..9424
                     /locus_tag="WM481_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9080781.1"
                     /translation="MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     NAGEGVLPKEKKDEEAAGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKN
                     EDAGAQNDMPQNAAESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSVVID
                     GPSQNITLTHCKGDPCNGDNLLDEEAPPKSEFESLSDEEKIKKYKKDGEKFTGLVAIK
                     VENNGLNKYTIIYQAQPTRSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYP
                     SGSRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKDRD"
     gene            9424..9627
                     /locus_tag="WM481_001487"
     CDS             9424..9627
                     /locus_tag="WM481_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9080782.1"
                     /translation="MLMPPEAQVFITAGRRVQIIKQAPEKGGIPKSGTEIAARIGGIP
                     QPAGETVGSSRNVGYFRPPFSGC"
     gene            9657..9935
                     /locus_tag="WM481_001488"
     CDS             9657..9935
                     /locus_tag="WM481_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080783.1"
                     /translation="MFRRPVFLTAVFQHSAGSNRQQQGDNALNPALRGGRVRAGLGKG
                     IAFPNRGGATGCALCAERVCGNGMPSEAGAFRRHCDVWGAGPVQIRIS"
     gene            complement(9928..10143)
                     /locus_tag="WM481_001489"
     CDS             complement(9928..10143)
                     /locus_tag="WM481_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080784.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDNGFIN"
     gene            complement(10136..10351)
                     /locus_tag="WM481_001490"
     CDS             complement(10136..10351)
                     /locus_tag="WM481_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFA9080785.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10554..11897)
                     /gene="argG"
                     /locus_tag="WM481_001491"
     CDS             complement(10554..11897)
                     /gene="argG"
                     /locus_tag="WM481_001491"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFA9080786.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKQ"
     gene            12022..12822
                     /locus_tag="WM481_001492"
     CDS             12022..12822
                     /locus_tag="WM481_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFA9080787.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDRTRQTAAAALDLPVVRHPEAAKFIEG
                     ITRKRGKPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYAGRFNQTERGSRSVYVFEQPESRITPIIEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAKLKNRVN"
     gene            12862..13848
                     /locus_tag="WM481_001493"
     CDS             12862..13848
                     /locus_tag="WM481_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFA9080788.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGKIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVSEALQAVR"
     gene            14509..14697
                     /locus_tag="WM481_001494"
                     /pseudo
     CDS             14509..14697
                     /locus_tag="WM481_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3090 a         3758 c         4437 g         3412 t
ORIGIN      
        1 tcttaaccat cccttccaac agccgtgcga aggttttttt atatcagtgg gaaatgcaat
       61 attctattgt ttttattgta gaattttaaa aacagattgt tgtgttccgc gtttttgccg
      121 gtttggaagc ggtggggcgc atcagccttt tataaaggca tatcggaagc ctgtataagg
      181 tttttgaaca tatcgatcct gttcccttgc aggcggacgg cagcggcgga tggcgaaagg
      241 cgggttttca aatgtccggt ttggcggcgc gtctttcaga cggcatggcg gcggagcgtg
      301 ccgcgccgtc tgaaaaccgt ttatttccct ttgccgtttc ctgcggagtc ttttttgtcc
      361 gaaccgaaga ggcggtcgaa acgtatggtg tatgtcagct cgccgcccga cgaacggctg
      421 ccgatacggg caaccgcctg tatggcgcgg gtcagccggt aaatcagttt gacggactgt
      481 tccgcgctgg agatgccgta ttcgtagccg atgtagagtt tgccggtcag ttgtttgccg
      541 acggtcagca cctgttcggc ggggttgagt tcgccggttt gcgcgttgcg gctgcgcttg
      601 ctggtaaagc ccaaatcatc caccagcccg atgcggtcgt tgatttgccc ggcaagcagc
      661 gcgcctgcgg ctgcggacag ggcggcattg tcgccgctgc tgccgctgcc ggcgcggttg
      721 aggatgagcc aggagagctt gtctttttca ctcatcggtt cgtttgccgt cagcgtaatg
      781 cgcgggctgt tgaggctgcc caatatttcc acgcccgcac cgacggggga aaggcggcgt
      841 tcggcgcgga tgttcaggtt ggggtcgttg agcgggccga caaaggagac tgtgcctttg
      901 gtaatgtcta aatcctgccc gtatgctttg taacgccctt tgatgacgcg gaccgtgccc
      961 accccacgca catttccgcc cggttgcgcg gtcagggtca gtttgccgcc tatggtaacg
     1021 tccgcgccgt agccggagaa gcggatgccg tcattgaggt ctaaagtcag gttcatattg
     1081 acggggagcg atgccgccgc ctctttcttg acttcgccca atacgacgac atcgtcgccg
     1141 acggacggca tcgaggattt ttgcgaaccg aacagcccct gatcagtttt aatcataccg
     1201 gtaacggata tgcctttttg cggcgaatag cgcaggcggg tgttgccgga aaccgtcagg
     1261 cggcggttgg ggcgggacag gatgcggtat ttgtcgaaca ccgcgccgat atcgacatcg
     1321 ggcacgctgt tttccatgct gaccgtgccg gagagttccg ccgtcccttc gtgccggaat
     1381 ttcaggctgt cgattaccca tttcctgcct gcaatatgcg aacgcagcga gccgttgtcc
     1441 aagatgatgc cttgggtttg gttgcggtaa tagagcttgt cgccgttgat actgccgccc
     1501 aagtgcgggt cggcgatgct gccgccgagg gttacggcgg cattcaggct gcctttgacg
     1561 gtttgtccga ccggtaggaa gttgcggaat acttcggcat cggcaacggt caggttgagc
     1621 ctgccgccca aaggcgcggt atcgaaggag cggctttgcc cgacggtgat attgccgttg
     1681 attttcccgt agttgctgct accgttgacg gcggcattga cggacggaga gcttacccgt
     1741 ccgccgattt gcgcggaggc attcaggctg ccggtaatgt tttgcgcggc ggcgggcaga
     1801 aagggcttca atgcgcccaa gtcgggaagg gaggctgtaa tcctgccgcc gagcggtgta
     1861 tttgccatat tgccgccgaa ggcgttgccg atgcccaaat cggcgttaat ccgtccgaaa
     1921 cgcgcgccgc cgtcaagcag gattccgatg cggtcgtttt gaaagcgcgt tttcagggaa
     1981 aatgcgttca aacccaaagc ctgcccgccg ggcaatacgg catcgccgct ttgccggctg
     2041 atattgaggt agccgcgcgc gttgtgcccg taggcaacat cccagtcgcc gtttaaaacc
     2101 agattgtgtt cgaagggcgg tttgaagaaa ttgtgcaact cggcgatgtg caggccgcgt
     2161 gcgccgcctt ttgccgatat gccggttttc ctgtcccaag aaaagtgttg caggttgagg
     2221 ctgccgccca ttgcctgcca atttgccgca cttgccgcca cgcgttccgc accggcttcg
     2281 agcgtcatac ggttttgcag cttgaggttg aatgcgccgc cgatgtcgag gatgccgatg
     2341 ctgcctttcc atcgggtaag ttccctgttg atgccgcctg aagcgtccaa atcgagtttg
     2401 aacggtttgc cgtccagcgt catggcggcg tgtgtgcgga tgcggtgctg cgcgcccgta
     2461 ccttccagcg tcaggccggc ggtatcgaca accgccgcgc cgcccgacag ggaaaggcgg
     2521 ccgcccttga tatcggcgcg catcgggcgg cttgtgtcgg gcgagccttt gagggtaaaa
     2581 tcgagcgaac ggatgtctgc cgctttgccg atgtgtaagt tgcgcgccgt gccggaaagg
     2641 tcggtttcaa aggttcggat gccgccgtcc aaatcgccgg aaaggtgtcc gcgtacattt
     2701 aaagaccccg cgagtccgaa accgaaacgg gataaatcgg gtgcggtgat attgaggtta
     2761 agccggtcgc cttttttgcc gaagccgccg tctgttttga cgatgttccg ccccaaccgc
     2821 aaatcgacgg cggcacgcgg aaggtggcgg gactcgtaaa caatgtcggc actgccggca
     2881 atcggcacgc cgttgaacgt accgggcaaa aaacgcattt tgcccgtaaa tttctctttt
     2941 gccagttcac cggcaagatg aatcgaaccg ttgatattgc ctgccggaaa ttgcggatcg
     3001 atgcgcgaag ggtcgaatgc gcgggaacgg atgtccagct tgagcaggcg gtctttaaac
     3061 agctcgagat agccttgcgc ggtcaggctg ccttccccgg cggagatgtt gacggtgtcg
     3121 aacaccagtt tccgctgttc gtttgcgggg tcgcttgcga tggcgaggct gccgtccgtg
     3181 cgtgccgtgc cggtgccgag ttgccaagag attttgggcg aggcggtcgt gccgccgatg
     3241 ccgatgctgc cgtccaacct gcctttgaac gcggtttgca gcacgtcttc cgcgccgacg
     3301 gagttgatgc ctatatttaa atcaaggatg tctttttcgg tgtcgatttt gcccgacagc
     3361 ctgatgccgc cccgtccgag cagggcggcg gacgtattgc cgatatgcac cgtgccgtcc
     3421 tgccggatga caaagccgcc caaaacctga cggacgggga tgccgttgcg gtcggcaaag
     3481 tcggctttgg tgttttccaa atcgagcgag ccttccagcg cgatgccgtc tgaaaacgac
     3541 gggatggcgg tcaggtcgaa attcagcccg gcatcgggca gggaaggcac gaaggcggac
     3601 ggattgatgt tgaatccttt gaccagtact tcttccaatg ttttatccaa tgattcggca
     3661 aacgggtgga tgacggattt tcccgagagg cggatattgc cgccgtcgat cgtcagttcg
     3721 gcgcgcacat ccttcaggct gccgctcagc cgcgccgtac tgtgtatggt ttcgccttcg
     3781 aatccgcctt tggtgtaaat ggcggtatcg agggcaaacg gttttttcaa gccgaccgag
     3841 gctgaccccg acgaactgct ccacggcgtg tcggcggcct tcaggtcgag gcggtgccct
     3901 ttacggtcgt aacggtatgc cgcgttgagg cgttcgagat agacagtttg tttgtcaaag
     3961 gttttgccca tgctgatttt gcccgtctcg aagcggtcga gatagacggt ggcgggcagg
     4021 tctatgctgt cgggcaggcc ttgaggcggg cgttcttctt taggcggagt cggtttggtt
     4081 acgatggcga tgtcgccggc ggagatgtcg gtgatgtgca ggctgcggcg catcagttcg
     4141 gacggtttcc acgcgaagcg gaagcggctg attttaaggt ctgccccctc ggtttctatc
     4201 gaccagttgt cgccgtcgaa gccgtcgagc agtgtgcctt tgaggttttg ggaggaaatg
     4261 tttacgccga accaggacgg gatttggtac agcccgaagc gcaaacctgc ttccgtaccg
     4321 gcgatccagc cgaggaaaca tactgccaaa atcaggacag acagcagtgc cgccgacagc
     4381 ttcagcagcg ggcggcgttt ttttgccggc ggggcggggc ggtgttcaga cggcattttg
     4441 cgcgtgccgt tttcggtcgg atcggtatct gtcggtgcgg tatcggtcat aatgtctgtt
     4501 tcaaaatggt ttgctgcaat gccgtctgaa gcggccgtat cggtttagaa acgcgttccc
     4561 aagctgatgt gccagcggat ttttttgtcg ctgtgtccgt aggcgatgtc gaaggaaaac
     4621 ggcgcgagcg ggctgaacca gcgcacgccc agtcccgaac cgtgtttcag cttcatacgt
     4681 ttgaaattgg cggcggcatc gcccatatcg tggaataccg cgccggacag ggtgcgcgta
     4741 aacggcagtt ggtattccaa gctgcccacc aagagggcgc gttcgggcag gaccgatccg
     4801 ttcggaccgg caagcccgat gctgtcaagt tcgtaaccgc gcacggaaga cgcgccgccg
     4861 ctgcggaaca tcagccccga ggggacatcg gcattgtcgc gtgcaacggt gtaacccgct
     4921 tgtccgcgta tgatgaacgt gccgagtttt ttgttttcgg gcgtgaagaa ataacctgcg
     4981 cgggcagagg tgcggattag cgcggtggag gacaggaatg tgcccaaagt cgtcccgatt
     5041 ttgccgtcga ggtaatggcc gttttcgggg tgcagcacgt tgttgagcag ctggcgtttc
     5101 caagaggcgg tcagcatcgt ggcgtggctg ttgcccaaat cgacatccga gccggggatt
     5161 ttccggcctt ctgcgagaaa ttccgccccc agcctggcat cgatgcccgc gcggtcgcgc
     5221 acataccaga tgccgccgga gaaggcgcgt ttttcaaggt tttgggtggt cgaacggttg
     5281 taggaaacgt tgcttgtcca gtagttgccc tgatagttgc gcggctggct gatgccggcg
     5341 gcaagcgtgg tttcgtattt gtccatatcc cagacgaccg agccgatata gcctttgttg
     5401 aagaggttgt aatagtcgta ggcgattttg ccgcccaaac cgtattccga atcgaggcgg
     5461 atgccggttt cgagtttgtg gcgtttgacc tcggttacgc tgactttgac ggggacgcgg
     5521 tcgccttgga ggcggtcgaa gtcggcttgt acggacgcgc cggaataatg cccgttttgt
     5581 tcgagcgcct gttggaagtc gagcagcagg tcgaggtcgt agggcgtgcc cggttggaag
     5641 cgcgccaggc cggagacggt ttgttcgggg taacgctgtg tgccggtgat ttcaaagtcg
     5701 ccgaaggcga tggggcggcc gctgtccacg acgacgttca aatcggcggt ggcggtatcg
     5761 gggttgacgg ccgcccgggt gttgccgagc ttggcaagcg ggtagccttt gcgcgttacc
     5821 gcgccgagga cggaagtttt gctgttttcc caactgtcct gatcgaaatc gctgcctacc
     5881 ggctgctgcc agttttccag cgcgttgcgg tagtattcgg cgaggttgcc gtctgaaagg
     5941 atgtcgccga ggatggcgac gccgacgttg gcgattttgg tgcgcgggcc cggtgtgatg
     6001 tgcaccgtat aagctccgtc tttttccgtc aggctgacct tgctgctgaa atagcctttg
     6061 ctgcggagca tggttttgac gttgtccggt gcttcttcgg caaggaatcc cgtctgttcc
     6121 ttatccaaaa cctcttcctg ctgctgcgtg atgagcggca ggtgttcttc gaccatatct
     6181 ttgatttcac tgtcctgcgt gtcgatgcgg acggggaatt tgggttttaa tttgactgat
     6241 tcggtgtcgg ggcttttgct tttgaacaat gcgaaacccg ccgccttgtt ttcggaaagg
     6301 tcggcggcag gcgcgtatgc gtgcggaaag aaaaataaag ccggcaggag cagggcggtc
     6361 ggtttgatca tcatggtacg ggtgtcgtgc atcggtgtgg cggatttcga ttctaacact
     6421 gttcggacgg ggcgggacgc ttcagacggc atactgccga cggtatttcg gaggttgcgg
     6481 cccgccggaa atgtatctgc ttgttttaag gcattttgtt tccaatccgc atatggaaaa
     6541 caggctgcgt ttcggaatag tgtcgaaaaa taggcaggat taaataaatt tattctcgtt
     6601 caataaatta aaccgttgcg ccgaatgata aaacaaagcc tgtccctgca tagggcaaat
     6661 caacgcacct caaaacacag cttgaaaaat aaaaaaaaca cagagaatga gttttctttt
     6721 aaaatacaac attttttaac atatcacaac ataaggaaga attatggcgt tcggcaaatc
     6781 cttatttcat gcgatcggcc gcgtcagcct ggtcaggcag attgccgccg gtttggcgtt
     6841 gggcatcgta atcggttcgg tttcaccgca actgggcttg gcggcaggct tgttcggcag
     6901 cctgtttgtc ggcgcgctca aagcggtcgc gccggttttg gtatttattt tggtggcggc
     6961 cacaatcgcg cagcaccaaa aaggcaacaa ggcgcatatc aggccgatta tcgtccttta
     7021 cctcatcggc acgttttccg cagccctgac cgccgtcatc gccggtatgg ttttcccgac
     7081 gcacattgtt ttggcgggcg cgggcgatgt gtccgccgcg ccgccttccg gcattgtgga
     7141 agtgttgaaa tcgctgctga tgaacctggt cgccaacccg attaacgcga ttgccaatgc
     7201 caactatatc ggcattttgg cttgggcttt ggttttgggc gcggcgttgc ggaatcacgg
     7261 ttcggacgtt acgcggcagg tcgttgccga tttggcggaa gcggtttcca ccgtcgtgaa
     7321 atggattatc cgttttgccc ctttgggtat tttcgggctg gtgtcgtcca caatcgcgga
     7381 gacgggtttc ggcgcgctgg cgggttatgc gaagctgctt gcggtgctgt tgggctgtat
     7441 ggcgtttatc gcgctggcgg tcaatcccgc catcgtgtgg tggaaaatcc gccgcaaccc
     7501 ttatccgctg gtgtttacct gcctgcgcga aagcggcgtg tatgccttct ttacccgttc
     7561 ttccgccgcc aatatccccg tgaatatggc tttggcgaaa aagctggggc tgcacgaaga
     7621 cacttattcg atttccatcc cgttgggggc gaccgtcaat atgggcggcg cggcgattac
     7681 gattaccgtt ttggctatgg cggcggcgca cacccaaggc atacaggttg attttgccac
     7741 cgcgctgctg ttgagcctgg tggcgacggt cagcgcgtgc ggcgcgtccg gcgtggccgg
     7801 cggttcgctg ctgctgattc cgctggcgtg cagcctgttc ggcatcagca acgatgtcgc
     7861 catgcaggtg gtcgccgtcg gcttcatcat cggcgtgatt caggattcgg cggaaacggc
     7921 gttgaattct tcaaccgacg ttttgtttac cgctgccgcc gatttgggcc gacagagaaa
     7981 ccgtgcggaa taaaccaagc tatgccgtct gaagcccgtt tggcgttcag acggcatatt
     8041 tttacaattt tatgggcata tggcggaaac aataccggct gccttgaata tgtttcaaca
     8101 cacagggcga tacataaagc gtcgccctat gaaggagatg atgatgttta aacgcagtgt
     8161 gattgcaatg gcttgtattt ttcccctttc agcctgtggg ggcggcggtg gcggatcgcc
     8221 cgatgtcaag tcggcggaca cgccgtcaaa accggccgcc cccgttgttg ctgaaaatgc
     8281 cggggaaggg gtgctgccga aagaaaagaa agatgaggag gcagcgggcg gtgcgccgca
     8341 agccgatacg caggacgcaa ccgccggaga aggcagccaa gatatggcgg cagtttcggc
     8401 agaaaataca ggcaatggcg gtgcggcaac aacggacaac cccaaaaatg aagacgcggg
     8461 ggcgcaaaat gatatgccgc aaaatgccgc cgaatccgca aatcaaacag ggaacaacca
     8521 acccgccggt tcttcagatt ccgcccccgc gtcaaaccct gcccctgcga atggcggtag
     8581 cgattttgga aggacgaacg tgggcaattc tgttgtgatt gacggaccgt cgcaaaatat
     8641 aacgttgacc cactgtaaag gcgatccttg taatggtgat aatttattgg atgaagaagc
     8701 accgccaaaa tcagaatttg aatcgttaag tgatgaagag aaaattaaga aatataaaaa
     8761 agatggagaa aagtttactg gtttggttgc tataaaagtt gaaaataatg ggttgaataa
     8821 atataccatt atttatcaag ctcagcctac tcgttctgca cggtcgagga ggtcgcttcc
     8881 ggccgagatt ccgctgattc ccgtcaatca ggccgatacg ctgattgtgg atggggaagc
     8941 ggtcagcctg acggggcatt ccggcaatat cttcgcgccc gaagggaatt accggtatct
     9001 gacttacggg gcggaaaaat tgcccggcgg atcgtatgcc ctccgtgtgc aaggcgaacc
     9061 ggcaaaaggc gaaatgcttg ttggcacggc cgtgtacaac ggcgaagtgc tgcatttcca
     9121 tatggaaaac ggccgtccgt acccgtccgg aagcaggttt gccgcaaaag tcgatttcgg
     9181 cagcaaatct gtggacggca ttatcgacag cggcgatgat ttgcatatgg gtacgcaaaa
     9241 attcaaagcc gccatcgatg gaaacggctt taaggggact tggacggaaa atggcggcgg
     9301 ggatgtttcc ggaaggtttt acggcccggc cggcgaggaa gtggcgggaa aatacagcta
     9361 tcgcccgaca gatgctgaaa agggcggatt cggcgtgttt gccggcaaaa aagatcggga
     9421 ttgatgttga tgccgcccga agcgcaggta ttcattacgg cgggacgacg cgttcaaatc
     9481 atcaaacaag cccccgaaaa aggcggcatc ccaaaatccg gcactgaaat tgccgcgcgt
     9541 atcggcggca ttccgcagcc tgccggggaa accgtcggca gcagccggaa tgtcgggtat
     9601 ttccgcccgc ctttttcggg ctgttgaaat atgaaaataa agcataggcc gtctgaatgt
     9661 tcagacggcc tgttttttta acggctgtgt ttcaacactc cgccggttcc aaccggcagc
     9721 agcagggaga taatgccttg aaccccgctc ttcggggcgg cagggttcgg gccggtttgg
     9781 ggaaaggcat cgcctttccg aatcggggcg gtgcaacggg gtgcgcctta tgtgccgaaa
     9841 gggtttgcgg caacggtatg ccgtctgaag cgggtgcgtt cagacggcat tgtgatgttt
     9901 ggggcgcggg tccggttcaa atccgcatca gttgataaac ccgttgtctt tcattttctt
     9961 ttcggaaaaa tactggccgc ataccagcag ggcggcgagg atgatgacaa acagccctgc
    10021 catagtcagt gtgattttga agaacaattc ggcttcgaga aaagaaaacc aaagttgcac
    10081 aatcagcagc agcacccagc cggcaaacag tccgagacag gcataaacgc cgagcttaaa
    10141 cacggtcccg ctccaaaatg acgacgtagg cttcgcgttt gaaaaacagc agcatacggc
    10201 gcaaatcttt ttccatcgtt acgacccgcc agccttcggg ggtttgtttg ttgaggaacg
    10261 cgctgaaatt gacggggttg acctttgccg cgccgaaaaa caggctggac aacaggcttt
    10321 cctgataaat gacgactttg tattctttca tcgtatttcc ttttcggttg aaaccccgcc
    10381 ctttggggcg gtaggatcag actttatttg ggagggaacc ccttccaaat caggacgata
    10441 cataggttgg tgctttatgt atcgtcctgt gtgttgaaac ataggattcc tttaatcaag
    10501 ggattgcctt ttcaggcaat cccttaaatt gcagcgatgc aatgcggcga aatttattgc
    10561 ttattgccca actgcggcaa taccgagcct tcgcccagcg agagcaaacc gctttgcgaa
    10621 taaataccca gtttggcgcg ggtgtcggtg atgtcgaggt tgcgcatcgt gagctgtccg
    10681 atgcggtcga gcggtgtgaa cgccgcacct tcgacttttt ccatactcag gcgttcgggt
    10741 tggtaggtca ggttgggcga ttcggtgttc agaatcgagt agtcgttgcc gcgccgcagt
    10801 tcgagggtaa cttcgccggt aacggctttg gcgacccagc gttgggcggt ttcgcgcaac
    10861 atcaaggctt ggctgtcgaa ccagcggcct tggtagagca aacgtccgag gcgcaggccg
    10921 ttgatgcggt attgttcgat ggtgtcttcg ttgtggatgc cggtaaccaa gcgttcgtag
    10981 gcgatgtgga acaatgccat gcccggggct tcgtagatgc cgcgcgattt ggcttcgatg
    11041 atgcggtttt cgatttggtc gctcataccc aagccgtggc ggccgccgat gcggttggct
    11101 tcgaggaaga gttcgacggg gtcggcgtat tctttgccgt tcaatgcaac cggcacgcct
    11161 tcttcaaagc gcacgctgac ttcttcgggt tcaatttcga cgttttcgtc ccagaaggca
    11221 acgcccatga tgggcttaac gattttgatg cccgagttca aaaattccaa gtctttggct
    11281 tcgtgggtcg cgccgagcat attggaatcg gtggagtagg ctttttcaac cgacattttg
    11341 tagttgaagc cgttggcaat cagaaattcg ctcatttcgt gacggccgcc gagttcgtcg
    11401 ataaattgct ggtcgagcca gggtttgtag attttcagcg cgggattggt gagcaagccg
    11461 tagcggtaga aacgctcgat gtcgttgcct ttgtaggtgc tgccgtcgcc ccagatattc
    11521 acatcgtctt ctttcatggc ggaaacgagc atggtgccgg tcacggcgcg gcccagaggc
    11581 gtggtgttga aataggcgat gccgccggtg gaaacgtgga acgcgccgca ttggatggcg
    11641 gcgatgcctt cgtgtgccag ttgtgcgcgg cagtcgatca ggcgggcgtt ttccgcaccg
    11701 tattccattg cttttttggg aatggcgttg taatcgtctt cgtcgggctg gccaaggttg
    11761 gcagtgtagg cataaggcag cgcgcctttg agtttcatcc acaacagcgc ggcagaggta
    11821 tcaagaccgc cggagaaggc gatgccgact ttctgaccga cggggaggtt ttgtaaaata
    11881 gtatggtttt ggttcatgaa aaaatcctcg atagcttgaa aaatcaaagc cattatctta
    11941 taaatagaaa atattgtcag ccatcatgcc gtctgaacgg gtattccgcc gtttcaggcg
    12001 gtatttaaaa ccgaaaacat tatgaacgcg ttcaacctca tcatcatcgg agacgaaatc
    12061 ttacacggca gccgccaaga caaacatttc gcctttttta aatccctgct cgaatccaaa
    12121 gggctgaagc tcaatcaggt gcaatatctg cccgacgaac ccgatttgct ggtcaggcaa
    12181 ctgcgccgca gcttttcaga cggcataccg acgttcgtta ccggcggcat cggcgccacg
    12241 cccgacgacc gcacgcgcca aaccgcagcg gctgctttgg atttgcccgt cgtccgccat
    12301 cctgaagccg ccaagtttat cgaaggcata acccggaaac gcggcaagcc gctcgattcg
    12361 cccgaacacg cccaacgcct gaaaatggcg gatttccccg agggcgcaga gcttgtgccc
    12421 aacccgttca acaacatcgc cggattttcc atccgcgagc attatttctt ccccggcttt
    12481 cctgtgatgg cgcacccgat ggcggaatgg gttttggaaa cttattacgc cggccgcttc
    12541 aaccaaaccg aacgcggcag ccgcagcgtg tatgtgtttg agcagcccga atcgcgcatt
    12601 acgccgatta tagagcatat cgagcaaact tatcccggcg tgcgttcgta cagcctgccc
    12661 agcgtcggtt ggacgcattc agacggcacg caggtcaaac cgcacattga gttcggcatc
    12721 aaagccgaag gagaagccgt caaccttttg gacgcggcgt gggcggaagt gttgcacagc
    12781 ttggacggat tgggagcaaa gctgaaaaat cgggtaaact gaaaactgtt tgcccaaatc
    12841 ggacgatatt taaggaaaac catgcaatac cgaatcagaa gagaaaacga agcacccgaa
    12901 gcaaaaaatg cgggggaaac cttatgggag cgcgacatta tgcgcgaagt gctgctgtcc
    12961 gcctatcggg acaggcgcag ggagcggatg tggaaaaaca tctggcgcgc cgtcagcacc
    13021 ctgattctgg ttgccctgat tgcaggcatt ttccgaaaag acgaagccgc attgcagttg
    13081 gcgggcaaca cgccgcatac cgcagtcgtc aacctgtacg gcaaaatcgg caacggcgta
    13141 gaagaccagg tcaaaaaact caaagacggg atggaggccg cctacaaaaa cccgcaggca
    13201 aaagccatcg tcatccgcgc caacagcccc ggcggttcgc ccgtcgtctc caacaccgct
    13261 tttgaagaaa tacgccgtct gaaggcgcag caccccggca tccccgttta tctcgtggca
    13321 gaggatatgt gcgcgtccgg ctgctactac atcgcggcgg cggcggacaa aatctatgcc
    13381 gacccgtcca gcatcgtcgg cagcatcggc gttatcggca gcagtttcga cgcgaccggc
    13441 ctgatggaaa aaatcggcgt aaaacgcagg gttaaaattg cgggcagcaa caaaggcatg
    13501 ggcgatccgt tttcgcccga aacgcccgaa cagtcgaaaa tctgggaaga aatgctgacc
    13561 ggcatacacg gcgaatttat caaagccgtc aaaaccggac gcggcggccg cctcaaattc
    13621 cgacaatatc ccgacgtgtt cagcggtcgc gtgtacacag gcgcggacgc gctgaaagtc
    13681 gggctggtgg acggactcgg caatatttac agcgtggcgc gagacgtggt caaagctccc
    13741 gatgtggtgg attacactcc gaaggacgat ttcggcagaa tcttgggcag acgcttcgga
    13801 gcagaactca aagcaagcgt ttcggaagct ctgcaggccg tccggtagcg ttgtttacag
    13861 tatttctaag ggaataccag aataaaaata tacacaaaaa cactcacctc cccagtatgc
    13921 tgccactaag tcagccgcgt tactatttac cgtgcactta aagccgcaaa aggcaggttg
    13981 tccccgggag gttttgtgca tcctgatacc gatcggttgc tgttgctgtt gctgttgctc
    14041 aaaggacgga aggccgccga taaacgagat tacctgtttg tcgctattga cgatttttat
    14101 actctgccat tttgccggac aaaaccgcag acagtgctgc caagtttctg accgaacatc
    14161 tggctgaccc ctgcttgtac ctggttgagt acgtttactc tgacaatgat aggtaatata
    14221 aagagccgtc caacatgctt tcggtgcagc ttgttatgat aatgggattg gttggaggct
    14281 tgcccgattt gcttgtccgc agaccaacgg taaggcggag cgggttatcc gtaccttgat
    14341 ggagatgtgg catgaggaac agtcgtttga cagtccggaa catcagcaaa aggagttgtg
    14401 ctgctttatt aatttaggcg ctgtcctaga taactaggga aattcaaatt aagttagaat
    14461 tatccctatg agaaaagccg tctaagccgg tataaacaaa ataaactcat tgggctattt
    14521 gtcgcaggtg taactgcaag aacagcggca gagttggtag gcgttaataa aaatactgca
    14581 gcctatgatt ttcatcgttt acgatgactg atttatcaaa acggtccgca tttggaaatg
    14641 tttgatggcg aagtagaagc agatgaaagt tattttggcg gacaacgcaa aggtaaa
//
LOCUS       DAWTCI010000053        14607 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWTCI010000053 DAWTCI010000000
VERSION     DAWTCI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.053"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            92..1615
                     /locus_tag="WM481_001495"
     CDS             92..1615
                     /locus_tag="WM481_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9080789.1"
                     /translation="MYRVCRLCAALCVWGAVGAYAAGLPDVRDDAAALRAQRAAAEGW
                     AGMPPEGDSAANGGSRVIDGDFLLSRPQLLEHVLRDALNGNQADLIASLADLYAKLPD
                     YDAVLYGRARALLAKLAGRPAEAVARYRALHRDNAADERVLLDLAAAEFDDFRLKSAG
                     LHFAEAAKLDLSAPVLENVGRFRKKTEGLTGWHFSGGISPSVNKNANNAAPQYCLQGG
                     GSRICSVTGPVRAAGLDYEAGAEKLTALADNHYLLFRANVGGTSYYFSRKSAYDDAFG
                     RAYLGWQYKNARQTVGVLPFYQAQLSGSDGFDAKTKPPADRRLAPYMLAHGAGVQLAH
                     SYRLSRRSQLSVSLERYRQRYREQGRAERNDGWQDGLYVSLARRLGGSATVFGGWQFA
                     RFVPKRETVGGAVNNAAYRRNAVHIGWAQEWGGTGGLNSRIAASYAHRNYKGIAAFST
                     EAQRNREWNVSLALSHDKLSYKGIVPTLNYRFGKTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1676..3550)
                     /locus_tag="WM481_001496"
     CDS             complement(1676..3550)
                     /locus_tag="WM481_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HFA9080790.1"
                     /translation="MPYFALFDDAVSRRAKLYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYEFGLPLMGMESERGGNIALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDEAGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIRAQALPDTSF
                     ADTLRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCSGGLGFEGAFNV
                     VIRTLSLKPVSDGIVSGIGGTDSNAQARTAGQGGETPHPFEANPPYRGVYGVGSGIVI
                     DSDPAAEYRECGWKARFLNELRPDFGIFETLRAENRQCALLDRHLCRLKTSAQALNLP
                     LPDGCENQIKQYIADLPDGAFRIKALLASDGISLSHAVLNRLTDKQRVIISPTILPAQ
                     NYLRRFKTTHRAIFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLTPS
                     LDLDILNGIMRQAVSDEPQKYLHTNQVIETHITQKTLQGAEEIRLSNALRGVFAAVLA
                     "
     gene            3721..4650
                     /gene="ppk2"
                     /locus_tag="WM481_001497"
     CDS             3721..4650
                     /gene="ppk2"
                     /locus_tag="WM481_001497"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HFA9080791.1"
                     /translation="MTDRQLQPFENVELGEKQDQLQVFEKAVLEHEGKGSAEDSGTAP
                     LPENYPCRKRMRRAVYEAEKAKLQIELLKVQSRVKDSGQRIVSLFEGRDAAGKGGTIK
                     RFMEHLNPRGARVVALEKPTTTERGQWYFQRYIQNLPTAGEMVFFDRSWYNRAGVERV
                     TGFCEPNEYMLFMCQAPELERMLVASGIHLFKFWFSVSREEQLRRFISRRDDALKHWK
                     LSPVDIQSLDRWDDYTEAKNAMFFHTHTGDAPWVIIRSDDKKRARLNCIRYFLHQLDY
                     PGKDVKAIGKVDDKIVLVPDTRYKEKTIDVGHD"
     gene            complement(4732..6036)
                     /gene="aldA"
                     /locus_tag="WM481_001498"
     CDS             complement(4732..6036)
                     /gene="aldA"
                     /locus_tag="WM481_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9080792.1"
                     /translation="MTAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAE
                     GGKTKDLARVEVMFTADYLDYQAEWARRYEGEIIQSDRPRENILSFKRPLGVIAGILP
                     RNFPFFLIARKMGPDLVTGNTIVVKPSSVTPINCHIFAEIIDAVGLPAGVFNVVDGPG
                     AEIGNALSAHPQVDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLD
                     LAVKSILASRVGNTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGAP
                     EMGTLIEERAVKAVAEKVERAVKQGAKLVCGGKRTEGRGYFFEPTLLTDTDNSMDIMK
                     EETFGLVLPVSAFDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINR
                     ENFEAMQGFHAGWKKSGIGGADGKHGLEEYLQTQVIYLETDI"
     gene            complement(5963..6163)
                     /locus_tag="WM481_001499"
     CDS             complement(5963..6163)
                     /locus_tag="WM481_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFA9080793.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGRADVD
                     RGARGATGLGASACGRTRRVFA"
     gene            complement(6271..7119)
                     /locus_tag="WM481_001500"
                     /pseudo
     CDS             complement(6271..7119)
                     /locus_tag="WM481_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221212.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7359..8159
                     /locus_tag="WM481_001501"
     CDS             7359..8159
                     /locus_tag="WM481_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9080794.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            8207..8983
                     /gene="mlaE"
                     /locus_tag="WM481_001502"
     CDS             8207..8983
                     /gene="mlaE"
                     /locus_tag="WM481_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFA9080795.1"
                     /translation="MNFIRSVGAKTLGLIQPFGSITLFLLNILAKSGTAFARPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTGQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCIPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            9034..9528
                     /gene="mlaD"
                     /locus_tag="WM481_001503"
     CDS             9034..9528
                     /gene="mlaD"
                     /locus_tag="WM481_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFA9080796.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAE
                     KAAE"
     gene            9559..10149
                     /locus_tag="WM481_001504"
     CDS             9559..10149
                     /locus_tag="WM481_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFA9080797.1"
                     /translation="MKKSSFISALGIGILSIGMAFASPADAVGQIRQNATQVLTILKS
                     GDAASARPKAEAYAVPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKFKNATVNVKDNPIVNKGGKEIVVRAEVGIPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGIDGLIAELKAKNGGK"
     gene            10200..10478
                     /locus_tag="WM481_001505"
     CDS             10200..10478
                     /locus_tag="WM481_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFA9080798.1"
                     /translation="MHTELKNGTLHIGGDITVKTLTGDAFGRFRQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            10475..11302
                     /locus_tag="WM481_001506"
     CDS             10475..11302
                     /locus_tag="WM481_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFA9080799.1"
                     /translation="MKKTAYAILLLIGFASAPAFAETRPADPYEGYNRAVSKFNDQAD
                     RYIFAPAARGYRKVTPKPVRAGVSNFFNNLRDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGVPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYPPKNIVFHTPAGRWGTTAAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQP"
     gene            11427..11789
                     /locus_tag="WM481_001507"
     CDS             11427..11789
                     /locus_tag="WM481_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFA9080800.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            11786..12172
                     /locus_tag="WM481_001508"
     CDS             11786..12172
                     /locus_tag="WM481_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFA9080801.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFLEEARWAFFEKRGLMHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGMRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            12169..12657
                     /locus_tag="WM481_001509"
     CDS             12169..12657
                     /locus_tag="WM481_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFA9080802.1"
                     /translation="MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(12738..12905)
                     /locus_tag="WM481_001510"
     CDS             complement(12738..12905)
                     /locus_tag="WM481_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFA9080803.1"
                     /translation="MEDDVWPGRRVIVMAGVTVGRGSVVGAGAVITKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(12872..13234)
                     /locus_tag="WM481_001511"
     CDS             complement(12872..13234)
                     /locus_tag="WM481_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFA9080804.1"
                     /translation="MESKFFFLLLRFAGSVLPPSHMRGIGIIGRRVLGFLARRISPHI
                     GRGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMGPECLFYSTNHKFDREKNALRA
                     TRKSVRLRWRTMSGRGAG"
     gene            13369..13584
                     /gene="rpmE"
                     /locus_tag="WM481_001512"
     CDS             13369..13584
                     /gene="rpmE"
                     /locus_tag="WM481_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFA9080805.1"
                     /translation="MKQGIHPNYREVNVTCSCGNKFVTKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            13842..14477
                     /locus_tag="WM481_001513"
     CDS             13842..14477
                     /locus_tag="WM481_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFA9080806.1"
                     /translation="MFYDSKCCYFNNPVFWDSRRLTYYPNVIFCQKKSRKDIINIYLG
                     QFLGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGESIAKEGL
                     RKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQ
                     KLAQVPSVGETLEKYSRWFVAVVYLGLGIYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         3193 a         3976 c         4084 g         3354 t
ORIGIN      
        1 gatgcgccgc gtccgccgcg cgtggggctt gcggcgtttc tgccgccgcc gtccggcggt
       61 gttttttaag gaaggcaaat tgaaggatag tgtgtatcgg gtatgccggc tgtgtgccgc
      121 gctgtgtgtt tggggtgcgg tcggcgcgta tgccgccggc ttgcccgacg ttcgggacga
      181 tgcggcggcg ttgcgcgccc agcgtgcggc tgcggagggt tgggcgggaa tgccgccgga
      241 aggggattct gccgcaaacg gcggttcgcg cgtaatcgac ggggatttcc tgctgtcgcg
      301 cccgcagttg ttggaacacg ttttgcgcga cgcgctcaac ggcaatcagg cggatttgat
      361 cgcttcgttg gcggatttgt atgccaagct gccggattat gatgcggtgt tgtacggcag
      421 ggcgcgggct ttgctggcga aattggcggg aaggccggcg gaggcggtgg cgcgctatcg
      481 ggcgttgcac agggataatg cggcagacga gcgggttttg ctggatttgg cggcggcgga
      541 gtttgacgat ttccggctga agtcggcagg gctgcatttt gcggaggcgg caaaattgga
      601 tttgtcggcg ccggttttgg aaaatgtggg gcgtttccgg aaaaaaacgg aggggctgac
      661 gggctggcat ttttcgggcg gcatcagccc ttctgtgaac aaaaacgcca acaatgccgc
      721 gccgcaatac tgcctgcagg gcggcggcag ccggatatgc agcgttaccg gacctgtccg
      781 tgcggcaggg ttggattatg aggccggggc ggaaaagctg actgcgctgg cggacaacca
      841 ttatctgctg ttccgcgcca atgtcggggg cacaagctat tatttcagcc gcaaatcggc
      901 ttatgacgat gctttcggca gggcttattt gggctggcag tataaaaatg cgcggcagac
      961 ggtgggggtt ttgccgtttt atcaggcgca attgtcgggt agcgacggct ttgatgcgaa
     1021 aacgaaaccg cctgccgacc gccggcttgc gccgtatatg ctggcgcacg gggcgggtgt
     1081 gcagttggcg cattcttacc gcctgagccg ccggtcgcag ctttcggtgt cgctggaacg
     1141 ttaccgccaa cgctaccgcg aacaaggccg ggcggaacgc aacgacggtt ggcaggacgg
     1201 gctttatgtg tcgctggcaa ggcggttggg cggctcggca accgtgttcg gcggctggca
     1261 gtttgcgcgg tttgtgccga aacgcgaaac ggtgggcggc gcggtcaaca atgccgccta
     1321 ccgccgcaac gccgtccata tcggttgggc gcaggagtgg ggcgggacgg gcggtttgaa
     1381 cagccggatt gccgcgtctt acgcccaccg caactacaaa ggcattgcgg cattttcgac
     1441 ggaggcgcag cgtaaccgcg aatggaatgt ctcgctggct ttgagccacg acaagttgtc
     1501 gtacaaaggc atcgtgccga cgttgaacta ccgcttcggt aagacggaaa gcaatgtgcc
     1561 gtatgcaaaa cgccgcaaca gcgaggtgtt tgtgtcggcg gattggcggt tttgaatggt
     1621 gggataatgc cgtccgaact ttgcggcagc aggttcggac ggcatttttg cgcgttcagg
     1681 caaggacggc ggcaaatacg ccgcgcaagg cgttggagag gcggatttct tcggctcctt
     1741 gcagtgtttt ttgtgtgatg tgtgtttcaa tcacttgatt tgtgtgcaaa tatttttgcg
     1801 gttcgtccga cacggcttgg cgcattatgc cgtttaaaat gtctaaatct aaagagggcg
     1861 tgagccattg tccgcgatgt ttgacgaaca cgttgctcct gccgccttcg agtaggatgc
     1921 cgtccgaatt gaaaaacagg ctgtcgaacg cgccttgcgt ttcggcggtt tgccacgctt
     1981 ggtcgaagat ggcgcggtgt gtggttttga agcggcgcag gtagttttgt gcgggcagga
     2041 tggtcggcga aatgatgacg cgctgtttgt cggtcagacg gtttaaaacg gcgtgggaca
     2101 ggctgatgcc gtctgaagcg agcagggctt tgatgcggaa cgcgccgtcg ggcaagtcgg
     2161 caatgtattg tttgatttga ttttcgcagc cgtcgggcag gggcaggttg agggcttggg
     2221 cggaggtttt cagacggcat aggtggcggt cgagcagggc gcattgcctg ttttccgcgc
     2281 gcagggtttc aaaaatgccg aaatcggggc gcagttcgtt gaggaatcgg gctttccagc
     2341 cgcattcgcg atattcggcg gcggggtcgc tgtcgatgac gatgccggaa ccgacaccgt
     2401 acacgccccg atacggcgga ttggcttcaa acggatgcgg cgtttccccg ccttgtcctg
     2461 ccgttcgggc ttgcgcgttt gaatccgtgc cgccgatgcc tgaaacaatg ccgtctgaaa
     2521 cgggtttgag cgacagggtg cggatgacga cgttgaacgc gccttcaaac cccaagccgc
     2581 cggaacacgg gttcaaatag ccgatgctgc ccgtataaag cccgcgcgct tcggtttcga
     2641 gcgattcgat aatctgcata ctcatttttt tgggcgcgcc ggtgatgctg ccgcagggga
     2701 aggcggcgcg gagggtgtcg gcgaacgagg tgtccggcag ggcttgcgcc cggatggtgc
     2761 tggtcatctg ccagacgctg ccgaaacgcg atactttaaa cggctcgggt acgcatactt
     2821 taccggtttg ggcgattttg ccgaggtcgt tgcgcagcaa atcgacaatc atcacgtttt
     2881 cggcgcggtt tttcgggtcg gtttgtaact cggcggcgcg gcgttcgtct tgtccgtcgc
     2941 ccaaaatcgg cgcggtgcct ttcatcggtt cggtgctgat ggtgccgtct gaagcgatgt
     3001 tgaggaagag ttcgggcgag aaacacagcg tccacgcgga ttgcccggct tcatcgggca
     3061 ggtgggacaa tacggcatag gggacgggct ggcgcaggcg gcggtagagg ctgacggggt
     3121 tgccgtaggc ttgcaggtgc aggcgggtgg tgtagttgat ttgataggtg tcgccgcgcc
     3181 ggatggcttc gtggatttgg cggatgcggt cgaggtaatc ggtttcggat acggaggatt
     3241 gcggcgtgga aataccggcg gggatgccgt ctgaatgtcg ggcaagccag ctttcggcat
     3301 cggtgtcggc gcagtcggca aaccagtgca gggcaatgtt gccgccgcgt tcggattcca
     3361 tccccatcag cggcaaaccg aattcgtagt ctgcaaacaa caccgaatgc agcccttttt
     3421 gccatcccga ttgcagcgcg ccgtctaaag catcgagttc ttcgggacgg aaaaaacggc
     3481 tttccacatg attttgatag agttttgcgc gtcggctgac ggcatcgtca aacagggcga
     3541 aataaggcat ggcaattcgg ggcaaatgtt ttgattatac gcccctttta cacggatttt
     3601 cagacggcgg ggcaaattcg ggcaacaaaa tgtaatttta tgtagagaaa gcggtggcga
     3661 aagtgtaaaa ttgtgaaaac ggactttcat ttccaacctg aaatacaata ggagaccttt
     3721 atgacagacc gccagttgca accgtttgaa aacgtagaat tgggcgaaaa gcaagaccag
     3781 ctccaagtat ttgaaaaagc tgttttggaa catgaaggca aaggttctgc cgaagattcc
     3841 ggcacagccc cgctgcccga aaactacccc tgccgcaaac gtatgcgccg tgccgtatac
     3901 gaagccgaaa aagccaaact gcaaatcgag ctgctgaaag tgcaaagccg ggtcaaagac
     3961 tccggccagc gcatcgtcag cctgtttgaa ggccgggatg cggcaggtaa gggcggcacc
     4021 atcaaacgct ttatggaaca tttaaacccg cgtggcgcgc gcgtggttgc tttggagaaa
     4081 ccaaccacta ccgaacgcgg acaatggtat ttccaacgct acatccaaaa cctgccgact
     4141 gcgggcgaaa tggtattctt cgaccgctcg tggtacaacc gcgccggcgt ggaacgcgtg
     4201 acgggcttct gcgaacccaa cgaatatatg ctctttatgt gccaagcccc tgagttggaa
     4261 cgtatgcttg tcgccagcgg catccacctg ttcaaattct ggttctccgt atcccgcgaa
     4321 gaacaactgc gccgcttcat ctcccgccgc gacgacgccc tgaaacactg gaaactttcc
     4381 cccgtggaca tccagtcgct cgaccgctgg gacgactaca ccgaagccaa aaacgcgatg
     4441 ttcttccaca cccacaccgg cgacgcgcct tgggtcatca tccgttcgga cgacaaaaaa
     4501 cgcgcccgtt tgaactgtat ccgctacttc ctgcaccagt tggactaccc gggtaaagac
     4561 gtgaaagcca tcggcaaagt ggacgataaa atcgttcttg ttcccgatac gcgttacaaa
     4621 gagaaaacca tcgatgtcgg tcatgactga ttgccggtga agtaataaaa atgccgtctg
     4681 aacccgattt caggttcaga cggcattttc tatccgtgtg tcaaaccggc atcagatgtc
     4741 ggtttccaga taaatgactt gggtttgcag gtattcttcc aaaccgtgtt tgccgtccgc
     4801 gccgccgatg ccggattttt tccagcccgc gtggaagccc tgcatggctt cgaagttttc
     4861 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgttcag
     4921 gttggtggtg tacacggagc tggtcagacc gaattcgcag tcgtttgcca gggcgatgac
     4981 ctggtcgagc gtgtcgaaag cggaaacggg cagcacgagg ccgaaagttt cttctttcat
     5041 aatgtccata ctgttgtcgg tgtcggtcag cagggtaggc tcgaagaaat agccgcgtcc
     5101 ttcggtgcgt ttgccgccgc aaaccaattt cgcaccttgt ttgacggccc gttccacttt
     5161 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgtg cccatttccg gcgcgcctgc
     5221 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatc gcggcggtca ttttttcaat
     5281 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
     5341 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgct aaatccaaat ccgcgtcttt
     5401 caacacgatg gcgggggctt tgccgccgag ttccagcgag actttggtga tgttggcgga
     5461 agcggcttcc atcacttggc gacccgcttc aaccgagccg gtcaggctga ccatatcgac
     5521 ttgcggatgg gcggacaagg cgttgccgat ttccgcaccc ggaccgtcca ccacgttgaa
     5581 cacgcctgcg ggcagtccga ccgcatcgat gatttcggcg aagatgtggc agttgatcgg
     5641 ggttacgctg ctgggtttga cgacgatggt gttgcccgta accagatccg ggcccatttt
     5701 gcgggcgatc aggaagaagg ggaagttccg cggcaaaatg ccggcaatca cgcccagcgg
     5761 acgtttgaac gataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     5821 acggcgcgcc cattcggctt ggtaatcgag atagtcggca gtgaacatca cttccacacg
     5881 cgccaagtct ttggttttgc cgccttcggc aacgatggtg tcggtcagct cgtcggcgcg
     5941 tccgcgtatg ccttgggcga ttttacgcaa atacgcgccg cgttcgaccg caggcagacg
     6001 ctcccaagcc ggttgcgccg cgcgcgccgc ggtcaacgtc cgccctgccg cctttgggtt
     6061 cgcgggcgat ggtttcttcg gtggacgggt tcaatacgtc gcgccattcg ccgttgaaat
     6121 cgttttcaaa gcgtccgttg atgtacatgg ccaattgttt catttcgggc cctccagttt
     6181 tgtagtcgga tgtagttttg gtttattccc aaataaattg ccgtacaagt ttctttacac
     6241 gcggattttg ggtttcaagt caaatacggc ttaccggttt tcttttttag tttccgcagg
     6301 cgtgctgccg aaatattgcc tgaatgcctg aataaagctg gaaacgtggc gctagccgca
     6361 caaatacgcg gtttcgccca cgtttttgcc cccgttttgc aacagataga gcgcgtgctg
     6421 catttgtttg tggtgcagcc actcgcttgc cgtaatgccg aaatggtcgc gcatacggcg
     6481 ttgcagcgtc ctttcgctga tgttcagcgc ggctgtcagc cggttgactt ggtgtgcgcc
     6541 gccgtcgaac gcggcattca gggtgcggct gaagtcttca gacggcatag cgtctgcttc
     6601 cgccgtttgc cccgccgccg gctcgatgcc gtctgaaacc gtgtcccaca aatccgacag
     6661 cagccgcaac acgtccgcct cgcggcgcaa tgtttcgccc aaatgcccct ttgggacggt
     6721 ttgcaggcag gatgccgcca agccgcgcag gtttgggggc aaatcccata tcctgaccgg
     6781 ttcgcggtaa agcaggggtg cgaaacgcgc gtattccgga cgcggcagcc attgttccat
     6841 acctttaatg gtcattttga ccgttttgcc gccgcgataa agatagcggc tgaacaggac
     6901 ttcttccccg acggcaatta ggacaatctt gccgccgtcc gcatcgatgc ggaagcggcg
     6961 gcggttgatg ccgaagtcca aacgcccttc cagcaacagc acgaacgaca cataaggttc
     7021 ggcgaggcgg ctgctgcaaa aatcgcaccg tgccgttacc gtaccgccgt ggatggaaat
     7081 gccgttggac aaagtatcga aacggtagct gccctccaca taagccgccg ccgcttttga
     7141 ccagattgac gaaccggctg ctgttcaaat gttctgcttt ttccatagtt ttctgataaa
     7201 ggttatcatt tgaaagataa catttttcgc cacagcaaac aatccgccgc cgacaaaggc
     7261 attttcacaa cacttgccgt ccgtatgccg tctgaaaatt gcccgtcttc ccgataagcc
     7321 ttaaaataca cgccgtcaaa cctaccgtcc gcccgtacat gagcccatcc ccctttatcg
     7381 aaatgaaaga cgtcgccttc gcgtatggcg accgcccgat tctgaacgac atcaatttca
     7441 gcattccgca aggcaatttt gccgccgtga tgggcggttc gggcagcggc aaaaccacgc
     7501 tgatgaggct gattacagga cagattcgtc cgcagtccgg gcaggttttg attgaaggac
     7561 gggatttggc gggtttttcg gctgacgaac tctacgaaca ccgccgccgt atgggcgtat
     7621 tgttccaaca cggtgcgctg tttaccgatt tgtcggtatt cgacaatatc gcttttccga
     7681 tgcgcgaact gacgcaactt ccggaagcgg ttattcgcga tttggttttg ttgaaattga
     7741 acgcggtcgg tctgcgcggc gtggaaaacc tgatgccgtc cgagttgtcc ggcgggatgt
     7801 cgcgccgcgt cgcgctggca cgcacaatcg cgctcgaccc cgaaattatg ttgtacgacg
     7861 aaccgtttac cggcctcgac ccgatttcct tgggcgtgat tgcccacttg atcagccgcg
     7921 tcaacaaggc cttgcgttcg accagcatta tggtaacgca cgacattgaa aaatctttgg
     7981 aaatcgtcga tcaggtaatt ttcttggcgc acggcgaaat tatgttctcc ggctcgccgc
     8041 aggaaatgcg cgaactggat tcgccttggg tgcgccagtt tgtcggcggg ctggcagacg
     8101 gccccgtggc ataccgctat ccggcgcaaa cgtcgttgca gcaggatttg ctcgggtaaa
     8161 tgagcaggtg ccgtctgaaa ccgttgaacc ttacatatag aaatttatga attttatccg
     8221 ttccgtcggg gcgaaaaccc tcggccttat tcaacccttc ggcagtatca cgctgtttct
     8281 gctgaacatt ttggcgaaat ccggcacggc tttcgcccgt ccgcgcctga gcgtgcgcca
     8341 agtgtatttt gccggcgtgc tgtcggtgct gattgttgcc gtttcggggc tgttcgtcgg
     8401 tatggttttg ggtttgcagg gctatacgca gttgtcgaaa ttcaaatccg ccgatatttt
     8461 gggctatatg gtcgcggctt ctctgttgcg cgaactgggt cccgtgttgg cggcgattct
     8521 gtttgccagc agcgcgggcg gtgcgatgac cagcgaaatc ggtttgatga aaacgaccgg
     8581 acagctcgaa gcgatgaacg tgatggcggt caaccccgtc gcccgcgtgg ttgccccgcg
     8641 tttttgggcg ggcgtgtttt ctatgccgct tttggcttcg attttcaacg tcgcgggcat
     8701 tttcggcgcg tatttggtcg gcgtgagctg gctgggtttg gacagcggta ttttctggcc
     8761 gcagatgcag aacaacatta cgatacatta cgatgtaatc aacggtttga tcaaatccgc
     8821 cgcgttcggc gtggcggtaa cgctgattgc cgtgcatcag ggcttccact gcatcccgac
     8881 ttcggaaggc attttgcgcg ccagcacgcg cacggtggtt tcgtccgccc tgacgatttt
     8941 ggcggtcgat tttatattga ccgcgtggat gtttacagat tgacagactg tctgaagacg
     9001 aaacgcggaa catcggatat tcaaggaact ttaatgaaaa agaacatatt ggaattttgg
     9061 gtcggactgt tcgtcctgat cggcgcggcg gcggttgcct ttctcgcttt ccgcgtggcg
     9121 ggcggcgcgg cgttcggcgg ttcggacaaa acttacgccg tttatgccga tttcggcgac
     9181 atcggcggtt tgaaggtcaa tgcccccgtc aaatccgcag gcgtattggt cgggcgcgtc
     9241 ggcgctatcg ggcttgaccc gaaatcctat caggcgaggg tgcgccttga tttggacggc
     9301 aagtatcagt tcagcagcga cgtttccgcg caaatcctga cttcgggact tttgggcgaa
     9361 cagtacatcg ggctgcagca gggcggcgat acggaaaacc ttgctgccgg cgacaccatc
     9421 tccgtaacca gttctgcaat ggttctggaa aacctgatcg gtaaattcat gaccagcttc
     9481 gccgagaaaa acgctgaggg cggcaatgcg gaaaaagccg cagaataaac atacacatcc
     9541 attttcagga aagacatcat gaaaaaatcc tccttcatca gcgcattggg catcggtatt
     9601 ttgagcatcg gcatggcatt tgcctccccg gccgacgcag tgggacaaat ccgccaaaac
     9661 gccacacagg ttttgaccat cctcaaaagc ggcgacgcgg cttctgcacg cccaaaagcc
     9721 gaagcctatg cggttcccta tttcgatttc caacgtatga ccgcattggc ggtcggcaac
     9781 ccttggcgta ccgcgtccga cgcgcaaaaa caagcgttgg ccaaagaatt tcaaaccctg
     9841 ctgatccgca cctattccgg cacgatgctg aaattcaaaa acgcgaccgt caacgtcaaa
     9901 gacaatccca tcgtcaataa gggcggcaag gaaatcgtcg tccgtgccga agtcggcatc
     9961 cccggtcaga agcccgtcaa tatggacttt accacctacc aaagcggcgg caaataccgt
    10021 acctacaacg tcgccatcga aggcgcgagc ctggttaccg tgtaccgcaa ccaattcggc
    10081 gaaatcatca aagccaaagg catcgacggg ctgattgccg agttgaaagc caaaaacggc
    10141 ggcaaataac cgacaaacca taccgtccga gggcggtatg ggtttttcga acaaacgcta
    10201 tgcatacaga actcaaaaac ggaacactgc acatcggcgg cgacatcacc gtcaaaaccc
    10261 tgaccgggga cgcatttggg cgtttccggc aacaatgccg tctgaaagaa accattgccg
    10321 tcgatttcgg aggtgtcaaa cgtgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc
    10381 tgcgcggctg caaaggcagc gtcaggctga ccggcattcc cgaatccgtg cgcgcgctgt
    10441 ccgaactgta cgaaatcaaa gactggctga aatcatgaaa aaaaccgcct acgccatcct
    10501 cctgctgatc gggttcgctt ccgcccctgc atttgcagaa acccgccccg ccgaccctta
    10561 tgaaggctac aaccgcgccg tttccaaatt caacgaccaa gccgaccgct acattttcgc
    10621 ccctgccgcg cgcggctacc gcaaagttac gccgaaaccc gtccgcgccg gcgtgtccaa
    10681 tttttttaac aacctgcgcg acgtggtcag tttcggcagc aatatcttgc gtttggacat
    10741 caaacgcgca agcgaagacc tcgtccgcgt cggcatcaat accaccttcg gtttgggcgg
    10801 gctcattgat attgccggcg cgggcggcgt tcccgacaat aaaaacactt tgggcgacac
    10861 gtttgcctcg tggggctgga aaaacagcaa ttatttcgtg ttgcccgtct taggcccgtc
    10921 caccgtccgc gacgcgctcg gcacgggcat tacctctgtt tatccgccca agaatatcgt
    10981 tttccatacc cctgccggac gctggggcac gactgccgct gccgccgtca gtacgcgcga
    11041 aggcctgctc gatttgaccg acagtctgga cgaagccgcc atcgacaaat acagctacac
    11101 gcgcgacctc tatatgaaag tccgcgcacg gcagaccggt gcaacacctg ccgaaggtac
    11161 ggaagataac atcgacatcg acgaattggt cgaaagtgcc gaaaccggcg cggcagagcc
    11221 cgccgttcac gaagattccg tatccgaaac acaggcagaa gcagcagggg aagccgaaac
    11281 gcaacctgga acacaaccct aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc
    11341 ccttccgtcg gaacggacag tccgacacta cagattgggc tataatccct tttttgcgca
    11401 taccgcgcga cagcaaagga aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca
    11461 tccctttaga acctttttgg aactggctgc aaaccctgcc gggcaaccac ctcgacggac
    11521 tgaccctcga agacatccaa gccgatgcca attcctacct tgtccgcccg tgcgaaaccg
    11581 ccgacgaagt atgggacgaa atcgaagccc gtttcgaaga cattttcgcc gccgaacttg
    11641 ccgactggtg cgaagacgaa cgcgagtggc ccgcactcga tgccgacatt ttcaacgaat
    11701 ggttcgacat ccagctttct accgtcatca ccgacctcga acacgaaccg cttgcccgcg
    11761 aagccttcca acccatcaac ctgaactgat gaagctgacc gtccgcaatt accatctcga
    11821 cggctacggt catgtcaaca atgcacgcta cctcgaattt ctcgaggaag cgcgctgggc
    11881 gttttttgaa aaacgcggac tgatgcacga gttggcaggc ctcatactga ttgtcgcccg
    11941 catcgacatc cgatacagcc gtcccgccgt cgaaggcgac gtattgcagt tttcatgccg
    12001 cctgaaaacc cccggcatgc gccgcattgt gctgacccag acgattacac tgccaaacgg
    12061 caaaaccgcc gcagaagccg acatcaccct gatgcccgtc cacgccgcca cacaacgcac
    12121 cgtcagcctg cccgccaccc ttgcccgcgc actggaagcc ttgtccgaat gaaaaaaata
    12181 ctcaccgccg ccgccgtcgc actgatcggc atcctccttg ccaccgtcct catccccgac
    12241 agtaaaaccg cgcccgcctt ctccctgccc gacctgcacg gaaaaaccgt ttccaacgcc
    12301 gacctgcaag gcaaagtcac cctgattaat ttttggtttc cctcctgtcc gggttgtgtg
    12361 agcgaaatgc ccaaagtcat caaaacggca aacgactaca aaaataaaga tttccaagtc
    12421 ctcgccgttg cccagcccat cgatccgata gaaagcgtcc gccaatacgt caaagactac
    12481 ggactgccgt ttaccgtcat ttatgatgcg gacaaagccg tcggacaggc attcggcaca
    12541 caggtttatc cgacttccgt ccttatcggc aaaaaaggcg aaattctcaa aacctacgtc
    12601 ggagaacccg atttcggcaa actctaccaa gaaatcgata ccgcgctggc gcaatagcga
    12661 tgccgtctga agcgcaaaaa acagaaacga tgttcctgtt ttttcagatc atccgccccg
    12721 acacgttcag gcggcattca accttccggc agattctttt ttaccaccgc aggattgcct
    12781 gccgccaaag agtagggcgg aatgtctttt gtaattaccg cgcctgcgcc cacgaccgag
    12841 ccgcgtccga cggttacgcc cgccatcaca atcaccctgc gccccggcca gacatcgtcc
    12901 tccaacgtaa tcggacggat ttccgtgtag ccctcaaagc gtttttttca cggtcaaact
    12961 tgtggttggt cgaatagaac aggcattccg gccccatcat cacatttttg ccgaccacca
    13021 gtccacggca gatttcacag tttgccccga tgcccgagcc gtcgcccaaa accgtatccg
    13081 gaaacacata cgccccgcgt ccgatatgcg gcgaaatccg ccgcgccaga aaaccgagca
    13141 cccgtctgcc gatgatgccg atgccgcgca tatgcgacgg cggcagcacc gaaccggcaa
    13201 aacgcagcag caggaaaaag aattttgatt ccatcaaacc ttcctcaaaa gaacttttta
    13261 cattttatcg aatatcctct tgacgaaaca acagaaaata gcgagaatgc cgagcttgtc
    13321 tgaggcatag gtacatcccc tatgcccgct tttttgaaag gaaatcaaat gaaacaaggt
    13381 attcacccga actaccgcga agttaacgtt acctgctctt gcggcaacaa attcgtaacc
    13441 aaatccgcaa tggaaaaaga aaacttcaac atcgaggttt gctccctgtg ccacccgttc
    13501 tataccggca cccaaaaaat cgtcgatacc accggccgcg tggacaaatt caacaacaaa
    13561 ttcggcaacc tgttcaaacg ctgattgccg ctttgcaaga attaagccct gccatcattt
    13621 cgatggcagg gctttttaat atttggcaca ctgacttctt atctatatag tggattaaca
    13681 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcgaggcaa
    13741 cgccgtaccg gtttttgtta atccactata ttttgacggg gttgaagttg acagctacaa
    13801 agtttaatgt ttgaatacat taaaaaatat atttgaatgg gatgttttat gattcaaaat
    13861 gttgttactt caataatcct gtattctggg acagccgtag acttacttat tatcctaatg
    13921 ttattttttg ccaaaaaaaa agcagaaaag acatcattaa catctattta ggacaatttc
    13981 taggctctgt tagcctgata ttgctaagtt tgctttttgc atttgtctta gattatattc
    14041 ctagtaaaga gattttaggt ttgctcggct tgattccgat tttcctaggc ctcaaagttt
    14101 tgctgttagg agattccgat ggagagtcta ttgccaaaga gggtttgcgc aaagataata
    14161 aaaacctgat ttttctagtc gctatgatta cttttgcaag ttgtggcgct gacaatattg
    14221 gtgtctttgt cccatatttt actaccttaa atttagcgaa tttgatagtg gctttactta
    14281 cctttctagt tatgatttat ctcttggttt tttctgccca aaaattggca caagtccctt
    14341 ctgtcggaga aactttggaa aaatatagca gatggtttgt tgccgttgtt tatttaggat
    14401 tggggatata tatcctgatt gaaaacaaca gttttgatat gctatggact gtgtcgggcc
    14461 aggaaaaaat attatgaaaa agatagtatc tgcaaagact gccccgtgca gtcttttttg
    14521 ttgccggtct ttttgtgtct gatgccgtct gaagcagtct ctgcacgacc tttgtgcgaa
    14581 tatttgctac acttggcaat ccgtcca
//
LOCUS       DAWTCI010000054        14275 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWTCI010000054 DAWTCI010000000
VERSION     DAWTCI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14275
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.054"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            316..1191
                     /gene="prpB"
                     /locus_tag="WM481_001514"
     CDS             316..1191
                     /gene="prpB"
                     /locus_tag="WM481_001514"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFA9080807.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVVGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLMVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLKMYRQFADAVKVPVLANITEFGATPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFDQKLDKLFQK"
     gene            1276..2424
                     /gene="prpC"
                     /locus_tag="WM481_001515"
     CDS             1276..2424
                     /gene="prpC"
                     /locus_tag="WM481_001515"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFA9080808.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSHRGYD
                     ILNLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLETLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPDSEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETVGGHFLHLLHGKRLTESQVKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSC
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEADIRERIGRKEIVIGFGHPVYTISDP
                     RNVVIKEVARGLSREAGDMRLFDIAECLESVMWEEKKMFPNLDWFSAVSYQKLGVPTA
                     MFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            2576..3364
                     /locus_tag="WM481_001516"
     CDS             2576..3364
                     /locus_tag="WM481_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFA9080809.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKSLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIVATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            3441..6046
                     /gene="acnD"
                     /locus_tag="WM481_001517"
                     /pseudo
     CDS             3441..6046
                     /gene="acnD"
                     /locus_tag="WM481_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
     gene            6596..7758
                     /gene="prpF"
                     /locus_tag="WM481_001518"
                     /pseudo
     CDS             6596..7758
                     /gene="prpF"
                     /locus_tag="WM481_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
     gene            8070..9269
                     /locus_tag="WM481_001519"
     CDS             8070..9269
                     /locus_tag="WM481_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFA9080810.1"
                     /translation="MSDQLILVLNCVSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGRRIAHGGEKYHESVL
                     IDQDVLDELKACIPFAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTGMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAVKN
                     GKSVDTGMGFTPIEGLVMGTRCGDTDPGVYSYPTFHAGMDVAQVDEMLNEKSGFPGIS
                     ELPNDCRTLEIAADEGREGARLALEVMTCRLAKYIASMAVACGSVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            9380..10000
                     /locus_tag="WM481_001520"
     CDS             9380..10000
                     /locus_tag="WM481_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HFA9080811.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIINQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDAPARALHPKRKTIGLRIPDNAVAQALLEELGGPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTDPTTVIDMTDGTELVRRGFGDTAVFGL"
     gene            complement(10162..10605)
                     /locus_tag="WM481_001521"
     CDS             complement(10162..10605)
                     /locus_tag="WM481_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFA9080812.1"
                     /translation="MYLTQHTDYGLRVLIYTAANDDALVNIATIASTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPERINIGAVVRHLEPMQLVECMGPNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYESKIPIAVR"
     gene            10779..11147
                     /locus_tag="WM481_001522"
     CDS             10779..11147
                     /locus_tag="WM481_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFA9080813.1"
                     /translation="MKPLKRHSALVGLSREHHHSLSLCVRMLRTPGADHRAELELHFA
                     ELETHFREEEAKFAPIWQNVAPELKTRFEGDHAKLRQMMASPECGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            11192..12346
                     /locus_tag="WM481_001523"
     CDS             11192..12346
                     /locus_tag="WM481_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFA9080814.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTAFWLAARIAAFIPGWG
                     AAASGILGTLFFWYGAVCMALPVIRSQNRRNYVAVFAIFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGMRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAIL
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNSIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(12404..13276)
                     /locus_tag="WM481_001524"
     CDS             complement(12404..13276)
                     /locus_tag="WM481_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFA9080815.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVINRASVVIDADSIGGADLVLIATPVATVPAVLTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRNGAQAAQFGLFRHRKLIITPHGGEDSDGI
                     ALVENLWRAVGADIFTMDAQHHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            13361..14173
                     /locus_tag="WM481_001525"
     CDS             13361..14173
                     /locus_tag="WM481_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFA9080816.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTGKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGCDGVRTGLYHKMHLFGFSGLGERYAEADTIRAGREVPHLSAEGMPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKHRVLDAV"
BASE COUNT         3218 a         3888 c         4003 g         3166 t
ORIGIN      
        1 gacattccag tcttgtgttt tagattaccg aaaaataaaa ctacataata ctacaaagga
       61 acattactat gaaaccaatt cagatgtttt tcccttttct gaataatccc cttgttttct
      121 tcttgtctgc ggttttgccg cataattccg aacggtctgc tgtttttctt tgattcgttt
      181 taaatatcaa taagataatt tttcccatat atttttaatg attgggatgc ccgacgcgtc
      241 ggatggctgt gttttgccgt ccgaatgtga tggaagcctg tccatactga aaaaagtcta
      301 taaaggagaa atatgatgag tcaacactct gccggagcac gtttccgcca agctgtgaaa
      361 gaatcgaatc cgcttgccgt cgtcggttgc gtcaatgctt actttgcacg attggccacc
      421 caaagcggtt tcaaagccat ctatctgtcc ggcggcggcg tggcagcctg ttcttgcggt
      481 atccctgatt tgggcattac cacaatggaa gatgtgctga tcgacgcacg acgcattacg
      541 gacaacgtgg atacgcctct gatggtggac atcgatgtgg gttggggcgg tgcattcaat
      601 attgcccgta ccattcgcaa ctttgaacgc gccggtgttg cagcggttca catcgaagat
      661 caggtagcgc aaaaacgctg cggccaccgt ccgaacaaag ccatcgtttc caaagatgaa
      721 atggtcgacc gcatcaaagc tgccgtagat gcgcgcgttg atgagaactt cgtgattatg
      781 gcgcgtaccg atgcgctggc ggtagaaggt ttggatgccg ctatcgaacg cgctcaggct
      841 tgtgtcgaag ccggtgcgga catgattttc cctgaagcca tgaccgattt gaaaatgtac
      901 cgtcaatttg cagatgcagt gaaagtacca gtattggcca acattaccga gtttggtgct
      961 acaccgcttt atacgcaaag cgagttggct gaaaacggcg tgtcactggt gctgtatccg
     1021 ttgtcatcat tccgcgcagc aagcaaagcc gctttgaatg tttacgaagc gattatgcgc
     1081 gatggcactc aggcggcggt ggtggacagt atgcaaaccc gtgccgagct gtacgagcat
     1141 ctgaactatc atgccttcga tcaaaaactg gataaattgt ttcaaaaatg atttaccgct
     1201 ttcagacggc ctttcaacaa atccgcatcg accgtctgaa aaataaaacc cataaaaata
     1261 caaaggagaa ataccatgac tgaaactact caaaccccga ccttcaaacc taaaaaatcc
     1321 gttgcgcttt caggtgttgc ggccggtaat accgctttgt gtaccgttgg ccgtaccggt
     1381 aacgatttga gccatcgcgg ttacgacatt ctaaatttgg cacaaaaatg cgagtttgaa
     1441 gaagtcgcac acttgctgat tcacggccat ctgcccaaca aattcgagct ggccgcttat
     1501 aaaaccaagc tcaaatccat gcgcggcctg cctatccgtg tgattaaagt tttggaaacc
     1561 ctgcctgcac atacccatcc gatggacgtg atgcgtaccg gcgtatccat gctgggctgc
     1621 gttcatcccg aacgcgaaag ccatcccgac agcgaagcgc gcgacatcgc cgacaaactg
     1681 atcgccagcc tcggcagcat cctgctctac tggtatcaat attcgcacaa cggcaaacgt
     1741 attgaagttg aaagcgacga agagaccgtc ggcggccact tcctgcacct gttgcacggc
     1801 aaacgcctga ccgaatcgca ggtaaaagcc atgcacgttt cgctgattct gtatgccgaa
     1861 cacgagttca acgcctccac ctttactgcc cgcgtgattg ccggtacggg ttcggatatg
     1921 tattcctgca tcaccggcgc catcggcgcg cttaaaggtc cgaaacacgg cggtgcgaac
     1981 gaagtcgcct acgacatcca aaaacgctac cgcaacgccg acgaagccga catccgcgaa
     2041 cgcatcggcc gcaaagaaat cgtgatcggt ttcggccatc cggtttacac catttccgac
     2101 ccgcgcaacg tcgtgattaa agaagtggcg cgcggtttga gccgggaagc gggcgacatg
     2161 cgcctcttcg acatcgccga atgcttggag agcgtgatgt gggaagagaa aaaaatgttc
     2221 ccgaatctgg actggttctc tgccgtttcc taccaaaaat tgggcgtacc gaccgctatg
     2281 ttcacaccgc tgttcgtgat ttcccgtaca accggttgga gcgcacacgt cctcgagcaa
     2341 cgcaaagacg gcaaaatcat ccgtccgagc gcaaactaca ccggccctga agatttggcg
     2401 tttgtggaga ttgaagaacg ataattgaag aatgcaatag cagtttgttc tttatttcgg
     2461 tatgcaaagc tgaggatttc agacgacctt gccttattgg aaaggttgtc tgaaataagt
     2521 ttaatctaat aggagaagat aatcctgtat tggcgcaagt aacaggataa gaaacatgga
     2581 agatttatac ataatactcg ctttgggttt ggttgcgatg atcgccggat ttatcgatgc
     2641 gattgcgggc gggggtggtt tgattacgct gcctgcactc ttgttggcag gtattcctcc
     2701 cgtgtcggca attgccacca acaagctgca agcagccgct gctacgtttt cggctacggt
     2761 ttcttttgca cgcaaaggtt tgattgattg gaagaaaagt ctcccgattg ccgcagcatc
     2821 gtttgcaggc ggcgtggtcg gtgcattatc ggtcagcttg gtttccaaag atattttgct
     2881 ggcggtcgtg ccggttttgt tgatatttgt cgcgctgtat tttgtgtttt cgcccaagct
     2941 cgacggcagt aaggaaggca aagccagaat gtcttttttt ctattcgggc tgacggttgc
     3001 accgcttttg ggtttttacg acggtgtgtt cggaccgggt gtcggctcgt tttttctgat
     3061 tgcctttatt gttttgctcg gctgcaagct gttgaacgcg atgtcttaca ccaaattggc
     3121 gaacgttgct tgcaatcttg gttcgctatc ggtattcctg ctgcacggtt cgattatttt
     3181 cccgattgtg gcaacgatgg cggtcggtgc gtttgtcggt gcgaatttag gtgcgagatt
     3241 tgccgtccgc ttcggttcga agctgattaa gccgctgctg attgtcatca gcatttcgat
     3301 ggctgtgaaa ttgttgatag acgagagaaa tccgctgtat cagatgattg tttcgatgtt
     3361 ttaaaccctt tcagacgact ccttcaaaac atcggctgaa acccaaacca caagaaaaat
     3421 agatccacag gagaaccgac atgactgcca accaaagtta ccgcaaaccg ctgcccggta
     3481 cggatttgga atactacgac gcgcgtgcgg cgtgtgagga catcaaaccc ggctcttacg
     3541 acaagctgcc gtacacgagc cgcattttgt ctgagaactt ggtcaaccgc gcggacaaag
     3601 tcgatttgcc gatgctgcaa agctggctgg gccagttgat agaagggaag caggaaatcg
     3661 attttccgtg gtatcccgcg cgggtggtgt gccacgatat tctggggcag accgcgctgg
     3721 tggatttggc aggcttgcgc gatgcgattg ccgaaaaagg cggcgatcct gccaaagtga
     3781 atccggtggt gcagacgcag cttatcgtcg accactcttt ggcggtggag tgcggcggtt
     3841 acgatcctga tgccttccgc aaaaaccgcg aaatcgaaga cagacgtaac gaagaccgtt
     3901 tccacttcat caactggaca aaaaccgcat ttgaaaatgt ggacgtgatt ccggcgggca
     3961 acggcatcat gcaccaaatc aatctggaaa aaatgtcgcc cgtcgtccaa gtcaaaaacg
     4021 gcgtggcttt ccccgatacc tgcgtcggca cggactcgca tacgccgcac gttgatgcgc
     4081 tgggcgtgat ttccgtggcg tgggcggttt ggaagcggaa accgtgatgc tgggacgcgc
     4141 gtccatgatg cgcctgcccg atattgtcgg cgtggagctg acgggcaaac ggcaggcggg
     4201 cattactgcc acagacatcg tgttggcact caccgaattc ttgcgtaaag agcgcgtggt
     4261 cggggcgttt gtcgaatttt tcggcgaggg cgcgagaagc ctgtctatcg gcgaccgcgc
     4321 gaccatttcc aacatgacgc cggagttcgg cgcgactgcc gccatgttcg ccatcgacgc
     4381 gcaaactatt gattatttga aactgaccgg acgtgacgac gcgcaggtga aattggtgga
     4441 aacctacgcc aaaaccgcag gcttatgggc aggtggcttg aaaaccgccg tttatccgcg
     4501 cgttttgaaa tttgatttga gcagcgtaac gcgcaatatg gcaggcccga gcaacccgca
     4561 cgcgcgtttt gccaccgccg atttggcggc gaaagggctg gcgaagcctt acgaagagcc
     4621 ttcagacggc caaatgcctg acggtgcagt gattattgcc gcgattactt cgtgtaccaa
     4681 tacttccaac ccgcgcaacg ttgtcgccgc cgcactgttg gcacgcaatg ccaaccgcct
     4741 cggcttgaaa cgcaaacctt gggtgaaatc ttcgtttgcc ccgggttcaa aagtagccgg
     4801 aatctatttg aaagaagcag gcttgttgcc cgaaatggaa aaactcggct tcggtatcgt
     4861 cgccttcgca tgtaccacct gtaacggcat gagcggcgcg ctcgacccga aaatccaaca
     4921 agaaatcatc gaccgcgatt tgtacgccac cgccgtattg tcaggcaacc gcaacttcga
     4981 cggccgtatc catccgtatg cgaaacaggc tttcctcgct tcgcctcctt tggtcgttgc
     5041 ctacgcattg gcaggtagca tccgtttcga tattgaaaac gacgtactcg gcgttgcaga
     5101 cggccgcgaa atccgcctga aagatatctg gccgacagac gaagaaatcg atgccatggt
     5161 tgccgaatat gtgaaaccgc aacaattccg cgacatttat atcccgatgt ccgacaccgg
     5221 cacagcgcaa aaagcaccaa gcccgctgta cgactggcga ccgatgtcca cctacatccg
     5281 ccgtccgccc tattgggaag gcgcactggc aggggaacgt acattaagag gtatgcgtcc
     5341 gccggcgatt ttgcccgaca acatcaccac cgaccacatc tcgccatcca atgcgatttt
     5401 ggccggcagt gccgcaggcg aatatttggc gaaaatgggt ttgcctgaag aagacttcaa
     5461 ctcttacgca acccaccgcg gcgaccactt gaccgcccaa cgcgcaacct tcgccaatcc
     5521 gaaactgttt aacgaaatgg tgagaaacga agacggcagc gtacgccaag gttcgttggc
     5581 acgcgttgaa ccagaaggcc aaaccatgcg catgtgggaa gccatcgaaa cctatatgaa
     5641 ccgcaaacag ccgcttatca tcattgccgg tgcggactat ggtcaaggct caagccgcga
     5701 ccgggcggcg aagggcgtgc ggctggcggg tgtggaagcc atcgccgccg aaggtttcga
     5761 gcgcatccac cgcaccaacc tcatcggcat gggcgtcttg ccgctgcaat tcaaacccgg
     5821 caccaaccgc cataccctgc aactggacgg tacggaaacc tacgacgttg tcggcgaacg
     5881 cacaccgcgc tgcggcctga ccctcgtgat tcaccgtaaa aacggagaaa ccgtcgaagt
     5941 tccggttacc tgccgccccg atactgcaga agaagtattg gtatatgaag ccggcggcgt
     6001 gttgcaacgg tttgcacagg actttttgga agggaacgcg gcttagagtt cgtctgaaaa
     6061 gcaagacgta gcgtgggtcg ggttctcatt cacgtaattc ccgatatggc aggcatttac
     6121 tgtaaatcgt cattcccgcg caggcgggaa tccagaaagt ggaattgagg aaaccttttt
     6181 atccgatgag tttctgtgcg gataaatctg gatttccgcc tacgcgggaa tgacggggtt
     6241 taataatcta ccgtatcaca acacagcagc cgtagattgg ggcgaacccc gacagtttgc
     6301 ggaatcaaac ggctttggtc ggagtggtag cctaatgtac ttctggaaag tgggtgtagc
     6361 gtgggctttg cccacgaaat aaaggctgaa ttgacatggt atagcggatt aacaaagatc
     6421 aggacaaggc ggcgggccgc aggcagtaca aatggtacgg aaccggttcg cccggtgctt
     6481 gggcgcctta gggaatcgtt ccctttgagc cggggcgggg caacccgtac cggtttaaat
     6541 taatccacta taaaaatcgt gggcagagcc cacgctacat aaggagaacc ctaaaatgcc
     6601 gcaaatcaaa atccccgccg tttactaccg cggcggtaca tctaaaggca ttttcttcaa
     6661 acgcaccgat ctgccggcag ctgcgcagga agcaggcggg gcacgcgaca aaatcctctt
     6721 gcgcgtactc ggcagccccg acccttacgg caaacagatt gacggtttgg gcaacgccag
     6781 ttcgtccacc agcaaagctg tgatttcgga caaatccgaa cgcgccgatc acgatgtcga
     6841 ttacttgttc ggacaagttt ccatcgacaa accatttgtc gattggagcg gcaactgcgg
     6901 caaccttacc gccgccgtgg gcacattcgc catcgaacag ggcttggtcg ataaatccaa
     6961 aatcccttca gacggcatct gcacggtcaa aatctggcag aaaaacatcg gcaaaaccat
     7021 tattgcccat gtgccgatgc aaaacggagc agtgttggaa acaggcgatt tcgagcttga
     7081 cggcgtaatt ttccggcagc cgaagtacaa atcgaatttt tagatccagc cgacggcgaa
     7141 ggcagtatgt tcccaaccgg caatttggtc gacgaaattg atgtgccgaa tataggtcgt
     7201 ctgaaagcca cgctcatcaa cgcgggcatt ccgaccgttt tcctgaacgc cgccgacttg
     7261 ggctacacgg gcaaagagtt gcaggacgac atcaacaacg atgccgccgc gctggaaaaa
     7321 tttgaaacca tccgcgcata tggcgcgctg aaaatgggtt tgatcagcga cgtatccgaa
     7381 gccgccgccc gcgcgcacac gccgaaaccc gccttcgtcg cgcccgccgc cgattacacc
     7441 gcctccagcg gcaaaaccgt aaacgccgcc gacatcgatt tgccggtacg cgccctgagc
     7501 atgggcaaac tgcaccacgc tatgatgggc atcgcctcgg tcgccatcgc cgccgccgtg
     7561 ctcggtacgc tggtcaacct tgccgcaggc ggcggaacgc gtaaagaagt gcgcttcggg
     7621 catccgtcag gtacgctgcg tgtcggtgct gccgccgaat gtcaggacgg acaatggacg
     7681 gccgccaaag cggtcatgag ccgcagcgca cgcgtgatta tggaaggttg ggtgcgcgtt
     7741 cccgatgatt gtttttgaaa tcatgtagga cgcacattgc tgtccgctgt ttcagacggc
     7801 atttgattaa ggattaccat aaagccgaat aaccttaccg ccgtcattcc cacgcaggcg
     7861 ggaatccgca gccgatattc tgtttcctga tttttatcag agcctgcgtg tgagcaaata
     7921 tggattcccg cctgcgtggg aatgacgcta ggaaatacgg catacgcttt gcccaaagag
     7981 gccgtctgaa acacactccg cccaacgccc atcctttttc agacggcccc gcaccaaaaa
     8041 aacaaccaca aactacaagg agaaacatca tgtccgacca actcattctt gtcctgaact
     8101 gcgtcagttc atcgctcaaa ggcgccgtta tcgaccgcaa aagcggcagc gtcgtcctaa
     8161 gctgcctcgg ggaacgcctg actacgcccg aagccgtcat taccttcaac aaagacggca
     8221 acaaacgcca agttcccctg agcggccgca actgccacgc cggcgcggtg ggtatgctgt
     8281 tgaacgaact ggaaaaacac ggactgcacg accgcatcaa agccatcggc cgccgcatcg
     8341 cccacggcgg cgaaaaatat cacgagtccg tcctcatcga ccaagacgtc cttgacgaac
     8401 tgaaagcctg catcccgttc gccccgctgc acaaccccgc caacatcagc ggcatcctcg
     8461 ccgcgcagga acactttccc ggcctgccca acgtcggcgt gatggacacc tcgttccacc
     8521 aaaccatgcc ggagcgggcc tacacttatg ccgtgccgcg cgaattgcgc aaaaaatacg
     8581 ccttccgccg ctacggtttc cacggtaccg gtatgcgtta cgtcgcccct gaagccgcac
     8641 gcatcttggg caaacctctg gaagacatcc gcatgattat tgcccactta ggcaacggtg
     8701 catctattac cgccgtcaaa aacggcaaat ccgtcgatac cggtatgggt ttcacgccga
     8761 tcgaaggttt ggtaatgggt acacgttgcg gcgacaccga tccgggcgta tacagctatc
     8821 cgactttcca cgcagggatg gatgttgccc aagttgatga aatgctgaac gaaaaatcag
     8881 gtttccccgg tatttccgaa cttcccaacg actgccgcac cctcgaaatc gccgccgacg
     8941 aaggccgcga aggcgcgcgc ctcgccctcg aagtcatgac ctgccgcctc gccaaataca
     9001 tcgcttcgat ggctgtggcc tgcggcagtg ttgacgcact cgtgttcacc ggcggtatcg
     9061 gcgaaaactc gcgtaatatc cgtgccaaaa ccgtttccta tcttgatttc ttgggtctgc
     9121 acatcgacac caaagccaat atggaaaaac gctacggcaa ttcgggcatt atcagcccga
     9181 ccgattcttc tccggctgtt ttggtcgtcc cgaccaatga agaactgatg attgcctgcg
     9241 acactgccga acttgccggc atcttgtagc caaaaaaggg ggcgagtccg caaaaatgcc
     9301 gtctgaaagg ttttcagacg gcattttgtt tggcgtttgg gcgtacaata cggaaaacat
     9361 gacgataagg aaacaaacta tggcacaatt tttcgccgtt catcccgaca atccccaaga
     9421 acgcctcatc aagcaggcag tcgaaatcat caatcaaggc ggcgtggtgg tttatccgac
     9481 cgattcctgt tacgccttgg gttgcaaact cggcgataag gcggcgatgg aacgcatact
     9541 ctccatccga aaaatcgatt tgaaacacca cctgaccctg atgtgcgccg atttgagcga
     9601 gttgggcaca tacgccaaaa tcgacaacgt acagtttcgt cagcttaaag ccgccacgcc
     9661 cgggccttat acctttatct tgcaggcgac gaaggatgcg ccggcgcgcg ccctgcaccc
     9721 gaaacgcaaa accatcgggc tgcgtattcc cgataatgcc gttgcacaag ccctgctgga
     9781 ggagttgggc gggccgcttt taagctgcac cctgatgctg cccgaagacg gcgaaccatt
     9841 gaccgaccct tatgaaatcc gcgagcgttt ggaacacgcc gtcgatttgg taattgacgg
     9901 cggctggtgc ggaaccgatc cgactaccgt catcgacatg accgacggca cggaactggt
     9961 gcgccggggt ttcggcgata cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa
    10021 gcatcagccg ttcagacggc atcgcgcgcc ttgccggcgg cagcccgaaa tgccggcgcg
    10081 tatcgcgccc ggtcggaata tccgtttgaa acggcaaaca aaatgccgtc cgaacgcggg
    10141 tttgggcggc attttgatgc gttatcgcac cgcaatcggg attttcgatt cataaagcag
    10201 gtcgtaggtc ggcttgttga gcaggtcttg cagcgtgaaa ccgtccagat acgtgaaaaa
    10261 cgacttcatt gcaccgccga gtatgcccgt cagccggcag gacggcgtaa tcaggcattc
    10321 gttgttcggg cccatacact cgaccagctg catcggttcg aggtggcgga cgaccgcgcc
    10381 gatattgatg cgttcgggcg gcgcggccag cctcagcccg ccgcctttcc cgcgtacgct
    10441 gtgcaagaac ccgcctttga ccagcgcggt aaccactttc atcaaatggc ttttggaaat
    10501 gccgtaggtc gaggcgatgg tggcgatatt gaccagcgcg tcgtcgttgg cggcggtgta
    10561 gatgaggacg cgcagcccgt agtcggtatg ttgggtcaga tacatgattt tctcggtatg
    10621 gatcgttatt cttatcggta cggtttaagt ttcacggaaa ataccttaat ggttgaaacc
    10681 ctgtccgtcg gggcggtaga atgtagcctg tctgcggcgg tatgccgtct gaaacatccg
    10741 cgctaccgtt tgataatttg ttattgtaac ctaaatttat gaaaccgttg aaacgacatt
    10801 ccgcccttgt cgggctttcg cgcgaacacc accactcgct ttccctgtgc gtgcgtatgt
    10861 tgcggacgcc cggggcagac catcgtgccg aactcgagct gcattttgcc gaactggaaa
    10921 cccattttcg cgaagaagaa gccaagtttg ccccaatttg gcagaatgtc gcgcctgagt
    10981 tgaaaacccg ttttgaaggc gatcacgcca aactgcggca gatgatggca agccccgaat
    11041 gcggtaacgc ggcgtggaat accgcttttg ccacaaccct gcgcgaccac gcgcgctttg
    11101 aagaacgcga gctgtttccc gccgtcgaac cgtttttgcc ggcatgattc cgttttgcgg
    11161 caaatatatt aatgataaac aaggaacaca catgaaattt accaaacatc ccgtctgggc
    11221 aatggcgttc cgcccgtttt attcactggc ggcactgtac ggcgcattgt ccgtattgct
    11281 gtggggtttc ggctacacgg gaacgcacga gctgtccggt ttctattggc acgcgcatga
    11341 gatgatttgg ggttatgccg gtctcgtcgt catcgccttc ctgctgaccg ccgtcgccac
    11401 ttggacggga cagccgccca cgaggggcgg cgttctggtc ggcttgaccg ccttttggct
    11461 ggctgcgcgg attgccgcct ttatcccggg ttggggtgcg gcggcaagcg gcatactcgg
    11521 tacgctgttt ttctggtacg gcgcggtgtg catggctttg cccgttatcc gttcgcaaaa
    11581 ccggcgcaac tatgtcgccg tattcgcaat atttgtgctg ggcggtacgc atgcggcgtt
    11641 ccacgtccag ctgcacaacg gcaacctagg cggactcttg agcggattgc agtcgggcct
    11701 ggttatggtg tcgggcttta tcggcctgat tgggatgagg attatttcgt tttttacgtc
    11761 caaacggttg aacgtgccgc agattcccag tccgaaatgg gtggcgcagg cttcgctgtg
    11821 gctacccatg ctgaccgcca tactgatggc gcacggcgtg atgccttggc tgtcggcggc
    11881 tttcgcgttt gcggcgggcg tgatttttac cgtacaggtg taccgctggt ggtataaacc
    11941 cgtattgaaa gaaccgatgc tgtggattct gtttgccggc tatctgttta ccggattggg
    12001 gctgattgcg gtcggcgcgt cttatttcaa acctgccttc ctcaatctgg gcgtacatct
    12061 gatcggggtc ggcggtatcg gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg
    12121 tcatacgggc aattcgattt atccgccgcc caaagccgtt cccgttgcgt tttggctgat
    12181 gatggcggca accgccgtcc gtatggttgc cgtattttct tccggcactg cctacacgca
    12241 cagcatccgc acgtcttcgg ttttgtttgc actcgcgctg ctggtgtatg cgtggaaata
    12301 cattccgtgg ctgatccgtc cgcgttcgga cggcaggccc ggttgagtaa aaccgccgca
    12361 gatttcgggt ctgcggcggt ttgcttttca gacggcatgg cggtcagttg ccgtccagcc
    12421 agcggtcgcg cgtggttttg gcttcttcaa aatagcggta cagggcttcg cggtcgtcgg
    12481 cggtcaggat gtctgccaaa acgtccaact gtttgcccaa gccttgaacc agttgcagca
    12541 ggctgtcttt gttggcaagg cagatgtccg cccacacggc gggatggccg gaggcgatgc
    12601 gggtgaagtc ccgaaagccc gtggcggcga atttcagata ttcctgtccg tcggggtggt
    12661 cgagaatctg gtggacatag gcgaaggcgg tcaggtgggg catatgggag acggcggcga
    12721 aaaccgcgtc gtggtgttgc gcgtccatcg tgaaaatgtc cgcaccgacc gcgcgccaca
    12781 ggttttctac caatgcgatg ccgtctgaat cttcgccgcc gtggggcgtg atgatgagtt
    12841 ttctgtggcg gaacagcccg aactgcgcgg cttgcgcacc gtttctgtcc gaaccggcaa
    12901 tcgggtgtgc ggcgatgcag cggtgcaggc ggtcgggcag gcagcggcgg aaggcttcga
    12961 tgaccgaaga tttggtgctg ccgacatcgg aaatccaagt gtgttccggc aaaacggggc
    13021 gcagcgcggt taaaacggcg ggaacggtgg cgacgggcgt ggcaatcagt accaagtccg
    13081 caccgccgat gctgtccgcg tcgatgacga cggaagcccg gttaatcacg ccgcgttcca
    13141 atgcacgttc gaggttgtcg cggtcggtgt cgataccggt aacggtgcgg acgagtccct
    13201 gccttttgag gtcgagaacg aacgaaccgc cgatcagccc tacaccgatg agggcaatat
    13261 ggttcaaaat gggcatttgt gtaaacggtt ttcgcaaagt gccgccatgg tagcctatcg
    13321 gcggaatatg ccgcaaggtc ggcaggaaaa aggagaagaa atggacaaaa tcagagttgc
    13381 cgccgtgcag atggtgtcgg gcgtgtcgcc ggaaaccaac gtcgccgcca tgaaacgcct
    13441 ggtcgcacgg gcggcggagc agggtgcgga ttgggtgctg ctgcccgaat attgggtgct
    13501 gatgggcgca aacgataccg gcaaactcgc acttgccgag cctttgggcg gcggacgctt
    13561 tcagacggca ttgagcgaaa cggcgaaaga atgcggcgtg gtgctgttcg gcggaaccgt
    13621 gccgctgcaa agccccgaag cgggcaaggt catgaatact ttattggtgt acgggtgcga
    13681 cggcgtaagg acggggctgt accacaaaat gcacctcttc ggtttttccg gtttgggcga
    13741 acgctatgcc gaagccgata ccatccgcgc agggcgggaa gtgccgcact tgtcggcaga
    13801 aggcatgccg gtggcggcag gtatttgtta cgatgtccgc tttcccgaat ttttccgccg
    13861 ccaactgccg tttgacgtat tgatgctgcc ggcggcattt acccatacga cgggcaaggc
    13921 gcactgggag ctgctgctgc gcgcgcgcgc cgtcgaaaac caatgttacg tcgtggcggc
    13981 ggcacagggc ggtttgcacg aaaacggacg gcgcacgttc ggacacagta tgattgtcga
    14041 tccgtggggc gacgtgttgg acgtattacc cgaaggcgaa ggcatcgtta cggcagacat
    14101 cgatgccaac cgcctgaaca gcgtccgcaa ccgcctgccc gccttgaaac accgggtttt
    14161 ggatgccgtc tgaaggttca gacggcatcg gtgtcgggga atcagcgggc cgtagcaacc
    14221 gaaccgtcat tcccacggaa gtgggaatct aggacgcggg gtttcagtta tttcc
//
LOCUS       DAWTCI010000055        14226 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWTCI010000055 DAWTCI010000000
VERSION     DAWTCI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.055"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            826..1935
                     /locus_tag="WM481_001526"
     CDS             826..1935
                     /locus_tag="WM481_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9080817.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALLAGYESLASRPLCTQTAIMVCNAIKHPYETAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLVIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRCLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     GIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSF"
     gene            complement(2663..3475)
                     /locus_tag="WM481_001527"
     CDS             complement(2663..3475)
                     /locus_tag="WM481_001527"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFA9080818.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLGHDVKAMVIACNTIAAVAGRKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLDTGNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            complement(3435..3643)
                     /locus_tag="WM481_001528"
                     /pseudo
     CDS             complement(3435..3643)
                     /locus_tag="WM481_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
     gene            3664..4125
                     /gene="tsaE"
                     /locus_tag="WM481_001529"
     CDS             3664..4125
                     /gene="tsaE"
                     /locus_tag="WM481_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFA9080819.1"
                     /translation="MSDFSPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFALHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            4122..5372
                     /locus_tag="WM481_001530"
     CDS             4122..5372
                     /locus_tag="WM481_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFA9080820.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPTHAQVFALPPVGGFKDRLVVDLYPHGMDADDPMMALLNGS
                     LNKTLRGSPEADLAQNTTPRPGRGKNGRRPVIMLDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            5454..6272
                     /locus_tag="WM481_001531"
     CDS             5454..6272
                     /locus_tag="WM481_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFA9080821.1"
                     /translation="MKGMDKLRYQRDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSPAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPKPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLRYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSI"
     gene            complement(6318..7460)
                     /locus_tag="WM481_001532"
     CDS             complement(6318..7460)
                     /locus_tag="WM481_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFA9080822.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTEVRAFDGGVSCRGGL
                     EQAYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFAGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAAGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDNGRRIVQTALDNAHNA
                     GADDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLLPKRKIPLYNGNIDCRLFLIDMVQGSNR"
     gene            complement(7509..7931)
                     /locus_tag="WM481_001533"
     CDS             complement(7509..7931)
                     /locus_tag="WM481_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080823.1"
                     /translation="MNKNRKLLLAVLLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GNWQAARIRLPVCVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(7924..8733)
                     /locus_tag="WM481_001534"
     CDS             complement(7924..8733)
                     /locus_tag="WM481_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFA9080824.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLGSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFGEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWEGGPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAGGSGANVLMEAAQWQDRSEHAGSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHAVVRDETIGKNHE"
     gene            complement(8815..9192)
                     /locus_tag="WM481_001535"
     CDS             complement(8815..9192)
                     /locus_tag="WM481_001535"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFA9080825.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFCNIGIGHDALGKPEFFYGPALSEWLEEQG
                     ISRVSLSMADEGDTVLAFAVAEK"
     gene            complement(9242..9970)
                     /gene="pdxJ"
                     /locus_tag="WM481_001536"
     CDS             complement(9242..9970)
                     /gene="pdxJ"
                     /locus_tag="WM481_001536"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFA9080826.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAEFAKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAQ
                     SHAEQIRQFERIQNGAHFAGDLGLVVNAGHGLTIHNVTPVAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(10023..10766)
                     /gene="recO"
                     /locus_tag="WM481_001537"
     CDS             complement(10023..10766)
                     /gene="recO"
                     /locus_tag="WM481_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFA9080827.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPASVSWYGSQELKTLHRAEWMGGWRQPQGRALFSGLYVNELVLK
                     LTAREDPMSELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCRDTGALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLLIGTLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(10803..11930)
                     /gene="pheA"
                     /locus_tag="WM481_001538"
     CDS             complement(10803..11930)
                     /gene="pheA"
                     /locus_tag="WM481_001538"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFA9080828.1"
                     /translation="MLECTANYRSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     RLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKAIGSYPTAVL"
     gene            complement(11969..13006)
                     /locus_tag="WM481_001539"
     CDS             complement(11969..13006)
                     /locus_tag="WM481_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFA9080829.1"
                     /translation="MFGTAFGQVAGGAVSDIKGRKPVALTGLIVYCLAVAAIVFASST
                     EQLLNLRAVQAFGAGMAVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLAAPMVGAL
                     LQGLGGWRAIFVFLAAYSPVLPGLVQYFLPNPAVGGKIGRDVFGLVAGRFKRVLKTRA
                     AMGYLFFQAFSFGSMFAFLTESSFVYRQLYHVTPHRYAWVFALNIITMMFFSRVTAWR
                     LKTGAHPQSILLRGIVVQFAANPSQLAAVLFFGLPPFWLPVACVMFSVGTQGLVGADT
                     QACFMSYFKEEGGSANAVSGVFRSLIGAGVVMAATVMAATMTASASCGIALLWLCSHR
                     AWKENGQSEYL"
     gene            complement(13022..13300)
                     /locus_tag="WM481_001540"
     CDS             complement(13022..13300)
                     /locus_tag="WM481_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080830.1"
                     /translation="MPSEAVSGGISFGFAPPSDMLNLRPCRPSFATVSLFDLNGKTMS
                     PTLPPMSGKLMAVLMAVLVALMPFSTDAYLPAIPEMAQPLNADIHRIE"
     gene            complement(13290..14165)
                     /locus_tag="WM481_001541"
     CDS             complement(13290..14165)
                     /locus_tag="WM481_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9080831.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANASGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATFA
                     AGRLSRRDAQNGNAV"
BASE COUNT         3284 a         4072 c         3824 g         3046 t
ORIGIN      
        1 gtggctgttg gggttaaggt ttatgcaaac taatacggct tgctgccctc tctctaactc
       61 tctcccacgg ggagagagga ctatgatgcc caccggcggc ttgctattag gaatatctga
      121 atcgtcattc ccacgaaagt gggaatctag aacgtcaaat ctcaagaaac cgttttatcc
      181 gataagtttc tgttccgaca ggtccggatt cccgcctgcg cgggaatgac gaatccatcc
      241 atacggaaac ctgcatcccg tcattcccac gaacctacat tccgtcattc ccacgaaagt
      301 gggaatccgg ttttttgagt ttcagtcatt cccgataaat tgccttagca ttgaaggtcc
      361 ggattcccgc ctgcgcggga atgacgggat ttgaggtttc tgtttttaat tttctgtttt
      421 cacgggaatg acggtttaaa agttacccga aacctcaaaa aaagctgtgt tttaatatag
      481 tgttgataga cgtacttggc ctccatgtat tcaatcgtgg aaatctatat cttcgtcctc
      541 gccgaaatag tctatgcccg atatacaatt ttgatacaca aacttggaaa tatgggtatc
      601 gtcgccggag cgatagaatg cggacagtta tatatatata cggttttttt aggggagcgg
      661 gcagatgaag ggcggatttt gaattaaccc catgcaaatt gacttttgcg ggcggtttta
      721 ccgcccatat atttacaaaa gccaaatttc taaacatata tccttgatat atacacggcg
      781 taaacatata ctggaaacat ctttaaattt tccgaaattt taaacatgag caactggaaa
      841 cccgatattc cctataacga tttaccaccc ctgccgccaa aacaggatat tgaaagcaaa
      901 accatcctga aacgttgtat agccgcccgt gcatcccttg cccgtttaaa gcaggcggca
      961 gaattgatac cgaatcaagc catgctgatt aacacccttc ctgttatgga agcccgtgca
     1021 agttcggaaa ttgaaaacat cgtaaccacc acggacaagc tgtttcaatc cctgcaaatg
     1081 gatacggaac ggcaagaccc tgccacgaaa gaagccctgc aataccgcac cgccctgctt
     1141 gcaggctatg aatcactggc gagccgccct ttatgcacac aaaccgccat catggtctgc
     1201 aacgccatca agcaccccta cgaaacggcc atccgcaaaa caggcggcac agccctaaaa
     1261 ggaggtaaca gcggaaatgt tgtctatacc ccgcccgaag gagaagaaac catacgcggc
     1321 aagctggcaa attgggagcg gtttattcac gaaagcggcg atttagaccc gcttgtcatc
     1381 atggcggcgg cacattacca atttgaagcc atccacccgt ttacggacgg caacgggcgg
     1441 acgggacgca tattgaacag cctgctattg attgaaaaag ggcttttgga tttgcctatt
     1501 ttgtatttga gccgctacat catcgaaaac agggcggact attaccgcct gcttttaggc
     1561 gtaaccgaac ggcaggattg ggaaagctgg ataatctaca tcttagacgg cgtagccgac
     1621 accgccgatt ggacggtatc gaaaatagat gcgatacgct gcctgttcga gcagacacgt
     1681 caacacatac ggacacacgc acaaggaatc tacacgcacg aactggtaaa tcttctgttt
     1741 gagcagccat atacacgcat tgccaaccta gaagcggcag ggatagccaa acggcagacg
     1801 gcttctaagt acctgaaaga gctttcaggc ataggtgtgc tgcaagaaat cgccatcggc
     1861 agggacaaac tattcattca tccgcgccta atggaactat tgcggggaga gggcaacagc
     1921 tttacttcat tctaaccccc tcttcatccc tacatgacta acacgaaaca gggattttga
     1981 cacccgaacc ggtacccctt gtattttccc cgcgaaaagc cggcatccgc ccgcgcatca
     2041 tgggagcaac aaaacccctg cctaaaattt tgacttgtgc aaattagggg tatatttggg
     2101 gggtatattg aaaaatggct aaaataaaat gtttaataaa caaaatgttg aaacttaatt
     2161 tcgatagagc ctctgcatat cgtattgagg cgttaatgga atttgagaaa gctattttta
     2221 aataagaaaa ggtaactatt aaatagctac ctttctaaaa ttaaatatca acacatcgtt
     2281 aaaacacagc caaaaaaaac cgaaaccgaa cggtccggat tcccgcctgc gcgggaatga
     2341 cggatttcaa gattacggta ttgtcgggga tgatgggaaa cggtgggaat tgtgtaaaaa
     2401 atgccgtctg aagcctttca ggcggcattt gcggcattcg gacatttaaa aatcccgtca
     2461 ttcccgcgca ggagggaatc cgaatacatc cgcacagaaa cctgcatccc gtcattcccg
     2521 cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgac aagtttccgc
     2581 accgacaggt ctagattccc actttcgcgg gaatgacggg attttaggtc tctgtaatct
     2641 cgggcagctt tccgttatcg ttttaaccca aagacaccat ttcaatctgc tccatcgtcc
     2701 tgcccagaaa acgctcgccg atggttctga atttcaaagg aatatcgctg acgtaaaaac
     2761 ggtagtcggg attgttgttg ccggtatcga gcaatccttc ctgtgcaagg acgcgtgcgg
     2821 tttcttcggc cgtcgtaatc gcggaatcga ccagcgcgac attgtgcgcc tccctgccga
     2881 ttaagggctt gagcaaggga aagtgcgtgc agcccaacac cagcgtatcg atgccgtctg
     2941 caagcaatgg tttgaggtat tcgcatacgg tcaggcgggt aacttcgtgt tccaaccaac
     3001 cttcctccac caaagggacg agcagcggcg cggcctgcgt gcggacgagc gtgtcggggt
     3061 tgtccctgtg gatggcgcgc gcataagcat tgctgttgac tgtcgtattg gtggcgatga
     3121 tgccgatttt attgttgcgc gtcgttgcca gcgcggcttt ggcgccggcg gaaatcacgt
     3181 ccaaaacggg catattgccc gtcttttgac ggattttccg ccccgcaacc gccgcaatcg
     3241 tgttgcacgc gataaccatc gccttgacat cgtgtcccaa taaaaaatcg acaatctgca
     3301 tcgagaaatt ttcgatggtc gccttagatt tcgtcccgta aggcacgcgc gccgtgtcgc
     3361 cgaaataaat gatgttctcc atcggcagcc gttccatcag cgcgcgcaca ttggtcaaac
     3421 cgccgattcc cgagtcaaaa acgccgatgg gccgctgcct gccgatattt tccatccttt
     3481 ttccaatccg tcctaaacat acaatccgca tattgtacac gcgccgcttt ttcttgacag
     3541 ccatgccgtc tgaaagcagg atgcgtattt ggctgttata atgtacggca atgccgccgc
     3601 cgggcgcacg gttttccgac cgcctgcccc tccgatccgc cacataaaag ttgtaaagcc
     3661 cctatgtctg atttttcccc cgtttcccga ttccttgccg acgaagccgc cacactcgat
     3721 ttgggcgcgg cgtggtcttc ccgtttaaac gcaccgctgg tcatttacct cgaaggggat
     3781 ttgggtgcgg gcaaaaccac gctgacgcgc ggcatcctgc gcggattggg tcaccagggc
     3841 gcagtcaaaa gcccgaccta cgccatcgtc gaatcttatc cgctggaacg cttcgccctg
     3901 caccatttcg acctctaccg cttctcgttc cccgaagaat gggaagacgc ggggcttgac
     3961 gagctgtttg ccgcaaacag cgtctgcctg atcgaatggc cgcaacaggg aggcgaattt
     4021 accccgcctg ccgacatcac cgcaacattg acacacggcg gcggcggcag aaaatgcctg
     4081 ctgaccgccc ataccgaacg aggacgcgaa agcctgccgc tatgaccaaa ctgacacgaa
     4141 gacaaatcat ccgccgcacc gccggcacac tgttcgccct aagccccatc gcatccgccg
     4201 ttgccaaaac ggtacgcgcc ccgcagttta ccgccgcacg gatatggccg tcgcacacct
     4261 acacccgcct gacgctggaa agcaccgccg cgctcaaata ccagcacttc gcgctcgaca
     4321 acccgggcag gttggtcgtc gatatacaaa acgcaaacat caataccgta ttgcacgggc
     4381 tttcccaaaa agttatggcg gacgacccct ttatccgcag catacgcgcg ggtcagaaca
     4441 cgccgaccac cgtccgcctc gtcatcgacc tgaaacagcc cacccacgca caagtcttcg
     4501 cgcttccgcc cgtcggcggc tttaaggacc gcctcgtcgt cgacctctat ccgcacggga
     4561 tggatgccga cgatccgatg atggcgctgc tcaacggcag cctgaacaaa accctgcgcg
     4621 gctctccgga agccgacctc gcccaaaaca ccacgccccg acccgggcgg ggcaaaaacg
     4681 ggcgcagacc cgtcatcatg ctcgatccgg gacacggcgg tgaagacccc ggcgccgtca
     4741 gcccgggcgg tttgcaggaa aaacacgtcg tcctctccat cgcccgcgaa accaaaaaac
     4801 aattggaagc cttgggttac aacgtcttta tgacgcgcaa cgaagacgtg ttcatcccat
     4861 tgggcgtgcg tgtcgccaaa gggcgcgcac ggcgggcgga cgtattcgtc tccatccacg
     4921 ccgacgcttt caccagcccc tccgcgcgcg gaacgggggt ttatatgctg aacaccaaag
     4981 gcgcgaccag ctctgccgcc aaattcttgg aacaaaccca aaataatgcc gacgcggtcg
     5041 gcggcgtacc gaccagcggc aaccgcaacg tcgataccgc cctgctcgac atgacgcaaa
     5101 ccgccacact gcgcgacagc cgcaaactcg gcaaactggt gcttgaagaa ttgggcaggc
     5161 tcaaccatct gcacaaaggc agggtggacg aagccaattt cgccgttttg cgcgcacccg
     5221 atatgccgtc tatcctggtc gaaaccgcct tcctgtccaa tcctgccgaa gagaagctgc
     5281 tgggcagcga atccttccgt cggcagtgcg cccaatccat tgcctcgggt gtccaacgct
     5341 acatcaatac atccgtattg aagcggggtt gattgaaaag gcggtgtttc aaaatatagt
     5401 tgtcggatga aggcaggcat aaacctactt ccgccccaaa ccgcgttttt atgatgaaag
     5461 gtatggataa attgagatac caacgggatt ttttaaatat ccgccccatt tttacagccg
     5521 gcgaacaaga atacctgacc gaattgtccg atcggcttcc tttatctgtt ttaacggact
     5581 ccgtgcgtaa tattgaagaa atcggtatcg attttgtgta cagtcctgcc aaattggaag
     5641 gaaacactta taatcaatac gatacgcagg cattgcttaa actcgggcaa accgcaggcg
     5701 ggaagttata ttcggatgcc gtaatgttaa tcaacctgag ggaaagttac cggcacctgc
     5761 tgtccggttt ggacagcccc aagccgtttg actggttgga tttcttaaaa accacccaca
     5821 gcctgatttc ggaaaactta ctggaaaaag gctccggcgg cgtagtccgc cgcgacagcg
     5881 ttactataag cgggacagac tacacccctt tatccaaccc gcaaagtttg gatacggaat
     5941 tgaaatggtt gcttcaagaa gcccccaaaa tagaaaatcc tttcgaccgc gccgtttatc
     6001 tccataataa tttggcgtat ctccgatatt ttaaggactg caataaacgt actgccagaa
     6061 actgtatgac cttgtcgctg atgcgctccg gtttttttcc ttgcgtattc tcccccgaca
     6121 gttatcccgc ctatgccgag gctgtcgtag cctattacga aaccggcgat tacggtttat
     6181 tcaagaaata ttttatttcg gcatacgaaa ataccgtaaa taagtacggt ccccagcctg
     6241 atgtggatat tttccgaaat ttctccattt aaaaagccgt ctgaacattt ttcagacggc
     6301 atttttgtac actttcctca acggttcgat ccctgcacca tatcgatcag gaacaggcgg
     6361 cagtcgatgt tgccgttata aagcgggatt ttccgcttgg gcagcaggcg catgaatttg
     6421 ggcatttccc tgtcgccggt aaacatgccg acccgccagc cggcatagtg ttttttcaac
     6481 cacgtaccca actgcggata aagtgcctgc aaagcgcgga cttcctcaag gcgcacgccg
     6541 tagggcggat tggacaccat gatgccgttt tcgccgttcg gacggacgga ctgcgcgtcg
     6601 gcaacgctga aggaaacgat gtcgtccgct ccggcattgt gtgcgttgtc caatgccgtc
     6661 tgaacgatgc ggcggccgtt gtcgctgcct gcaatcgggg cgcggacggg gcgggtttgc
     6721 gcttcggcgc ggcggcgcaa atccgcccac agcgttttat cgaaattttg cagtttttca
     6781 aaaccgaagc ggcgcatcat acccggcgcg cggccggcgg caatccaagc ggcttcgata
     6841 gcaatcgtgc cgctgccgca aaacgggtct tgaaacggct gcgtgccgtc gtagcctgcc
     6901 gagagcagca gcccggcggc aaggttttcg cgcagcgggg cttcgccggt atccaggcgg
     6961 tagccgcgtt tgaacagggc ttcgcccgaa gtgtcgatga agatttcgac attgcgttcg
     7021 tccaaaaagg cgtggatgcg gacatcgggc gcggctttgt ccacgctcgg acgtgcgtcg
     7081 taaatgtcgc ggaaagcgtc gcagacggca tctttgacgg tcaggccggc aaattggagg
     7141 cttttgacgt tggcgcgttt tgcctcgatt ttgactttga acgtctgctg caaggtaaac
     7201 cagttgaacc agttgatgtt tttggcaagt ttgtagatgt cgcgctcggt tcggtatgtg
     7261 cctttggtca ggcgcagcag gatgcggccg gcggtgcgcg agtgcaggtt ggcggcgtag
     7321 gcctgttcca atccgccacg gcaggaaacg ccgccgtcaa acgcccgaac ctcggtgcag
     7381 ccgaggcttt cgagttcttg cgataaaacg gtttccaagc cgcgcgggca ggtggcgaaa
     7441 agtgtatata aagtattcat agcgtgtcct ttccggacgg atgttccggc aatggcggat
     7501 tgaaaggttt attcggcggt aaatgccgtc tgaaatgtcc ggctgccgat tgtaatgtcc
     7561 gccgtaaaat cgcgcctgcc ttcgacacag acgggcaggc ggatgcgtgc tgcctgccaa
     7621 ttccccgacg gttgccgctc gaacatatag cggttgaaac ccatatccat atttttcata
     7681 ctgaagctga tgctgacctg ttccgtgccg gcgggcgcgt gttcgatata aatatcaaac
     7741 ggttttttgg ttgaaacggc ggcggcgcgg acacggcttc cgtccggcag cgtgcaaccc
     7801 tcggtcaaat cgcattgcgc cgccacggct tgcggctgct gcgcctgcca ccattgcaac
     7861 aaaacgagct tgaaggcggc aaaggcagtc agcagcagta cggcaagcag taatttgcgg
     7921 tttttattca tgattcttcc ctattgtttc atcccttacc accgcgtgcg cccccaatcc
     7981 gagccatagt tttccataac gtgcaaccgt ctgtccgttt gctgcaagta cgagcggcac
     8041 ggatacgccc tgccgcaggg cttctgccgc ctcttgtgcc tgaacatcat cgcacacgcg
     8101 ccacacgacc acatccgcgc ccttttcctg cgcctgccgc cattgttcgc gggtctgcac
     8161 catcatccac acgctgccgg cgtgttcgga cctgtcctgc cattgtgccg cctccatcaa
     8221 aacgttcgca ccgcttcccc ctgccgaacc caaaggcagg acgcggtacg cgcccatact
     8281 gttttcgccg tacaaacctg ttttcaggct gccgtacaaa cggcgcggga cggacagcga
     8341 acgcaaaagc gcaccgttgg caggcagcat gggggcaacg gtaaaatcac ccgccttctg
     8401 ccaagaccat tcctgccctt cgcgcgattg cggtccgccc tcccattgat cggggtttac
     8461 ccataagaat ttcaggcaga cgcgggcgtg ttcgtaggaa tggattttgg tcagccaagg
     8521 cgtggcggcg aggatgcgga tgccgagttc ttctccaaac tcgcgttgca gggcttggaa
     8581 gtcggtttcg cccgcctcga ccttgccgcc ggcaaattcc caatatccgg catagggctt
     8641 gccttcgggg cgcgagccga gcaggtaatt gccgtctgaa tcgagcagga tgccggcaac
     8701 gacgcggata agggggcggg tatcttggat catggcgggt atgcggttgg tcggtcggaa
     8761 tgcaaatgag ggaagggcaa tgccgtcaac gggtttgccg aatttcagac ggcattattt
     8821 ttcggcaacg gcaaacgcca acacggtatc gccttcgtcc gccatactca ggctgacgcg
     8881 gctgatgcct tgttcttcca gccattcgga cagggcgggg ccgtagaaaa attcgggctt
     8941 gcccaatgcg tcgtgcccga tgccgatgtt gcagaaggaa accgcgcctc gtatgcccgt
     9001 gccgacggct ttggcgaagg cttctttggc ggcaaagcgt ttggcaaggt agttgacggg
     9061 tttgcccgcc tgcggaaatt caagcagctc ttcgggagtg agtatgcgcc ctgcaaacgc
     9121 ctgtccgaat tttttgttta agcggatgat gcgcttgagg gaaacgatgt ctgtaccgat
     9181 gccgtaaatc atatttgcgc tcctttttcg tgaaatgccg tctgaaacgg tttgcctgcc
     9241 cttacggcag cagccttgcc ctgaacatcg cctccttcat ctggcgcacg gcttcgggaa
     9301 gtccgaggaa gagggcttgg gcaatcagcg aatgcccgat gttcagttcg cggatggcga
     9361 ggatttgggc gacgggggta acgttgtgta tggtcagtcc gtgtccggcg ttgacgacca
     9421 agcccaaatc gccggcgaaa tgcgcgccgt tttggatgcg ctcgaactgc ctgatttgtt
     9481 cggcgtggct ttgtgcgtcg gcatatgcgc cggtgtgcag ctcgacaacg ggcgcgccga
     9541 catcataggc ggcttggatt tgcctgtcgt cggcatcgat aaacaaggac acgcgtatgc
     9601 ccgcgtcggt caggattttg gcgaattcgg cgattttttc ctgttgcgcc aatacgtcca
     9661 aaccgccttc ggtcgtgatt tcctgccgtt tttcaggcac gatgcacacg tcttccggca
     9721 tcactttaag cgcgttttcg agcatttctt ccgtcaacgc catttcaagg ttcaggcgcg
     9781 tgcggatggc gtttttgacg gcaaatacat ccgcgtcttt gatgtggcgg cggtcttcgc
     9841 gcaggtgcat ggtaatcagg tctgcaccgt gcgtttcggc aaccagtgcc gcctccacgg
     9901 ggctgggata agtcgtgccg cgcgcgttgc ggacggtggc gatgtggtcg atgttgacac
     9961 ccagaagcat aatgtttcct tttctcttcg ttttcctgtt tgacggcatg gtgcgggcgc
    10021 agctacgcgg tattgcggtc gaactgcctt atctgttcca acacctgccg cgatttcaag
    10081 ccttcgggaa gcagcgtgcc gattagcagc cgtgtgattt tcaatgcctg ttgcaggctt
    10141 tcggcggtgc ggaaactgcc ttcgcgcaaa tcgatcaggc tctgcccttc gacaattgcg
    10201 ccggcttcgt gcgaaagcgc gcctgtgtcc cggcatacgg gcatgaccgc ctcttcgggc
    10261 atcagccggt aggttttgtc cgccaaaata tcgccgcccg tgccgtcggc gtgcaaatcg
    10321 ggggcaacgc ccaaggcatt gagcagcttc cattcgaagc ggcgcaaatc ggcaatatgg
    10381 ttggcttcac ggcacactgc ctccatcact tttgccaacg cgtcgtacag ttcggacatc
    10441 gggtcttcgc gcgcggtcag tttcaacacc agctcgttca catacagacc gctgaacaac
    10501 gccctgccct gcggctgccg ccatccgccc atccattcgg cgcggtgcag ggttttgagt
    10561 tcctgactgc cgtaccacga cacgctggca gggacgaacg gaaccaatac gccgcgcagc
    10621 tcgctctgcc gtttgcgcgc gctgcgcgcc agcaaagcca cacgcccgta acggcggctg
    10681 aatgcttcaa cccgcaggct gctttcgcgc caaggcgaag atgccagcat aaaaacgggt
    10741 tcgtggttga ctcggtattc ggacataatc gttgtggaaa tgccgtctga acgctgccgc
    10801 gactacaaaa cggcagtcgg atacgaaccg atggctttga caaacgaagc gcgttcgccc
    10861 aagcgttcca atgccgtctg aatctgcgcg tcccggcggt gtccttcgat gtcgatgaag
    10921 aacaggtatt cccacaaaac ggatttgctc ggacggctct caaacttggt catggaaata
    10981 cccgattcgg tcagcggttg cagcagcgag gcaaccgcgc cggcgcggtt gggcgcggaa
    11041 acggccagcg aggttttgtc gctgccgctt gcaccggttt cgtgatgtcc catgaccaga
    11101 aagcgcgtgg tgttgttcgg ttcgtcttcg atgcactcgg caaccatatc gagtccgtaa
    11161 atttccgccg ccgtgcgtcc ggcgatggcg gcaaccgtac cgtcgtccga ttcggcaacc
    11221 agccttgcgg cttcggcatt gctggacacg gcaatccgtt cggcattggg cagacgcctg
    11281 cccaaccagt cgttgcactg cgccaacgcc tgcgcgtgcg aaaatacttt ggcgataccc
    11341 tcggtgctgc cgttgttttt gcgcaacagg ttgtggtgga tgcgcaaaac gacttcgccg
    11401 cacgcctgca acgcggtaac ggcaagcaaa tccaatgtgc gcccgaccga gccttcggtc
    11461 gagttttcca cgggcgcgac cagataatcc gcctgacggg tttcaacctg tttgaagcag
    11521 tcgtctatgg tcggacacgc cattgtatgc gcggcgtgtc cgaaatgctt gattgccgcc
    11581 tgttgggtaa acgtgccctg cggccccaga taggcaatgg tcagcggacg ttcgacggcg
    11641 aggcactcgc tcatcacttc ccgaaacagg cgtgctaccg attcgtcggg cagcgggcct
    11701 ttgttcaaat cctgaatgcg gcgcaacacg gcaacctcgc gctccgggcg gtataccgcg
    11761 cccgtgcctt tcagctcgcc gatggcgtgc gcgtgttgcg cgcgttcgtt gagcaggcgc
    11821 aggatttccg catcgatggt gtcgatggcg ttgcggtggg gaaggaggag ttcgtcgata
    11881 gtttgggaca taatttcgcc tgaacggtaa tttgccgtgc attctaacat caaatgccgt
    11941 ctgaagcggt ttcagacggc attttccgtt aaaggtattc gctttgcccg ttttctttcc
    12001 acgcacgatg cgagcagagc cacaaaagcg caatgccgca agaggcggac gcggtcatgg
    12061 ttgccgccat cacggttgcc gccatgacca cgcccgcgcc gattaaggac cggaatacac
    12121 ccgacacggc gttcgcgctg ccgccctctt ctttgaaata agacataaag catgcctgcg
    12181 tgtccgcacc gaccaggccc tgcgtaccga cggaaaacat cacgcacgcg accggcagcc
    12241 aaaacggggg caacccgaaa aacagcacgg cggcgagttg ggacgggttg gcggcaaatt
    12301 ggacgacaat cccccgcagc aggatgcttt gcggatgcgc gccggtttta agccgccacg
    12361 cggtaacgcg gctgaaaaac atcatcgtga tgatgttgag tgcaaacacc catgcgtacc
    12421 ggtgcggcgt aacgtggtag agctgccggt acacgaagga agattcggtc agaaaggcga
    12481 acatcgaacc gaagctgaat gcctgaaaaa acagataacc catcgcggca cgggttttca
    12541 atacgcgctt gaaccgcccc gccaccagcc cgaacacatc cctgccgatt ttgccgccga
    12601 cggcgggatt gggcaggaaa tactgtacca aaccgggcag caccggcgaa tacgccgcca
    12661 agaaaacgaa aatcgcccgc catccgccca atccctgcaa caatgcgccg accatgggtg
    12721 cggccagcgg cacaaccatc agaatgatgc cgataagggc aaacatctgc gcggctttgc
    12781 gtccggaata ataatcgcgc accatcgcac cgacgatgac tacagccatg cctgcgccga
    12841 acgcctgtac cgcacgcagg ttaaggagct gttcggtact cgaagcaaat acgatggcgg
    12901 caacggcaag gcaatataca atcaaaccgg tcagggcgac gggtttgcgc cctttgatgt
    12961 cggacaccgc gccgccggcc acttgcccga acgccgtgcc gaacataaac aaactcagac
    13021 tctattcgat acggtggata tccgcgttca gcggctgcgc catttcggga atcgcgggca
    13081 ggtaggcatc ggtggaaaac ggcatcagcg cgaccagtac cgccatcaaa accgccatca
    13141 gttttccgct catcggagga agggtcggag acatggtttt tccgttcaaa tcaaataaag
    13201 aaaccgtcgc aaacgacggt cggcacgggc gcaagtttaa catatcggac ggcggggcaa
    13261 aaccgaagga tatgccgccc gaaaccgctt cagacggcat tgccgttttg cgcgtccctg
    13321 cgcgacagcc ggccggcggc gaaagtggcg gcgatgacga caaacacgcc caaggcggac
    13381 acgggcgata aatgttcgcc caaaatcaaa accgccaaca gcacgccgac gacgggttcg
    13441 agcgaaatca acagtcccga cgcgttggca ggaacacggc tcatcccctt gttccacagc
    13501 caataggcgt accagccgca ccccaaaccc aaatacaaca gcgacaatac catcccgacg
    13561 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
    13621 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgggcaat cagcctttgc
    13681 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
    13741 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
    13801 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
    13861 acaaacacca tcagcagcgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
    13921 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
    13981 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
    14041 gcaggcagcg cggcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
    14101 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
    14161 aacatagtga gtggtaaagt cagatggtca tacaaaagta tagcggatta acaaaaatca
    14221 ggacaa
//
LOCUS       DAWTCI010000056        13445 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWTCI010000056 DAWTCI010000000
VERSION     DAWTCI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13445
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.056"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(263..1198)
                     /gene="hemC"
                     /locus_tag="WM481_001542"
     CDS             complement(263..1198)
                     /gene="hemC"
                     /locus_tag="WM481_001542"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFA9080832.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQSLQDGRADLAVHSIKDVPMDLPEGFALAAISERA
                     NPFDAFVSNRYARLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDERIRMILSESDSLPAAGQGALGIEIATHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLILRGLVGHPDGSIVLQADA
                     QAPAGYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            1317..1832
                     /gene="ybeY"
                     /locus_tag="WM481_001543"
     CDS             1317..1832
                     /gene="ybeY"
                     /locus_tag="WM481_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFA9080833.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENVSSAAGIPAERDFYRWAWSAL
                     KNEYLRADIGLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSERLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGTLHLMGYDHIKDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            1834..2658
                     /locus_tag="WM481_001544"
     CDS             1834..2658
                     /locus_tag="WM481_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFA9080834.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLTRLEKVLDFAELEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSVLRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDESADNIHSVSAERWRIHAATEIEDINAFF
                     GTEYGSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="WM481_001545"
     CDS             complement(2801..4180)
                     /gene="nhaC"
                     /locus_tag="WM481_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFA9080835.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQAGMIGALNQGMGAVYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTACATAGVAFMGMAAAFQADMAMTAGAIV
                     SGVFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAVRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(4470..5693)
                     /locus_tag="WM481_001546"
     CDS             complement(4470..5693)
                     /locus_tag="WM481_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFA9080836.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVIQGWAADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            6130..6543
                     /locus_tag="WM481_001547"
     CDS             6130..6543
                     /locus_tag="WM481_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFA9080837.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRNFPKHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLIRRFGIWLLAFAWLPVVGDALPLT
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            6590..7219
                     /gene="nth"
                     /locus_tag="WM481_001548"
     CDS             6590..7219
                     /gene="nth"
                     /locus_tag="WM481_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFA9080838.1"
                     /translation="MNRQIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKS"
     gene            7357..8856
                     /locus_tag="WM481_001549"
     CDS             7357..8856
                     /locus_tag="WM481_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFA9080839.1"
                     /translation="MFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFAQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKNGYILTNTHVVAGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKASGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKAKLGN
                     AAEHTGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGKHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            9059..10579
                     /locus_tag="WM481_001550"
     CDS             9059..10579
                     /locus_tag="WM481_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFA9080840.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWADGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TASPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTFGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFSTTGIVLAVLIFLLKDKKRADV"
     gene            10636..11721
                     /gene="nagZ"
                     /locus_tag="WM481_001551"
     CDS             10636..11721
                     /gene="nagZ"
                     /locus_tag="WM481_001551"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFA9080841.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDSEGAET
                     AETHAEHIGWVLATELSACGIDLSFTPVLDLDWGNCAVIGNRSFHRNPEAVARLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIILVCNRPDLVDELRDGFTIPDNQDLAGRWQYMENSLGHEAVQAVIQTTG
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            11946..12022
                     /locus_tag="WM481_001552"
     tRNA            11946..12022
                     /locus_tag="WM481_001552"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11980..11982,aa:Pro,seq:cgg)
     gene            complement(12358..12996)
                     /locus_tag="WM481_001553"
     CDS             complement(12358..12996)
                     /locus_tag="WM481_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFA9080842.1"
                     /translation="MKKISVAPENPQYRIVEIFESLQGEGRNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLKPAPPQIDYVATSPKACYAAKYETNCIAEADEVRIVADGDVLAFCENMERK
                     IRARHYYLSPCEQNGVMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(12993..13361)
                     /locus_tag="WM481_001554"
     CDS             complement(12993..13361)
                     /locus_tag="WM481_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFA9080843.1"
                     /translation="MKLLSTILVLLVAAEHFYIAWLEMTQIPGEKAAEMFKLPYEFME
                     QKRVQTLFGNQGLYNGFLAVGLLWAQFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAVLAV"
BASE COUNT         3378 a         3831 c         3378 g         2858 t
ORIGIN      
        1 aaaaaatacg aatatcatca ttctcaaatt caaaatttga acgccacagt attatccgaa
       61 gccacaaaaa cctttccgcc gtcattcccg cgcaggcggg aatctggaaa cccaatgctg
      121 caagaattga ttaaaaacaa ctgaaaccga gcaaactgga ttcccgcctg cgcgggaatg
      181 acgaaataag gtaagtagaa cttattaaat aaattaatta aaaacaataa cttatattat
      241 tttctttaaa ttttcgataa aatcaatttt ccgtattcaa tactgctcca atcaattcct
      301 gcgcaccgtc gtccgccagt tttttggcaa ccgcacgtcc aagcgcatcg gcatatccgg
      361 caggggcttg cgcgtccgcc tgcaacacaa tcgatccgtc ggggtgcccg accaatccgc
      421 gcaagatcag cagcccgttt tcttcagtgc aatacgcggc cagcggcact tggcagcttc
      481 cgcccaaagc gcgcgccaaa gcgcgttcgg cggtaacgca ggcgtgtgtg gtatcgtggt
      541 ttaacggctt caagacttcg tacaaatctt cgcgatgcgt ggcaatttca atacccaatg
      601 cgccttgtcc ggcggcaggc aggctgtcgg attccgacaa aatcatgcgg atgcgttcat
      661 ccaattccag acgctgcaac ccggcggcag ccaagataat tgcatcgtat tcgccgttgt
      721 cgagtttgga caaacgggtt tgcacattgc cgcgcaaagg tttgataagc aaatgcggat
      781 agcgcgcgcg caactgggct tcacggcgca ggctggatgt gccgacaacc gcgccttcgg
      841 gcatttcttc caaacgcgcg tatcggttgg acacaaacgc gtcaaacgga ttggcgcgtt
      901 cgctgatggc ggcaagggcg aagccttcgg gcaaatccat cggcacgtcc ttaatcgaat
      961 gcaccgccaa atcggcgcgc ccgtcttgaa gggattgttc caactctttg acaaacaagc
     1021 ctttcccgcc gacttttgac aaagttctgt ccaaaatccg gtcgccgcgc gtggtcatgc
     1081 ccaaaatttc gacttcgcaa tcgggataca gcgccttcag acggccttgg atatgtttcg
     1141 cctgccacat ggcaagcagg ctttcgcggc tggcgataac aagttttttc gggttcatgt
     1201 tcaatgcgtt caaagaatgg aaacggcgtg gagtctatca tattttgaac gtttcttcag
     1261 acggcaccgt caggtctatg ttatatttac gcccacaaaa cacgaaaccc gccacaatga
     1321 aacgcgccaa aaaataccct ttcttaacgt tgcagcggca acgtttccat ttgaactttg
     1381 aaaacgtctc ttccgccgcc ggcatccccg ccgaacgcga tttttaccgc tgggcgtggt
     1441 ctgccttgaa aaacgaatac ctccgcgccg acatcggttt gattcttttg gacgaagaag
     1501 aagcccgcgc ctacaaccgc gactaccggg gcaaagatta tgccaccaat gtattgagtt
     1561 tcgcgctcaa cgaaggcgaa atcctgccct gccaagtttc ggaaagactg tacggcgatt
     1621 tgattatctg cccgcaagtc gttttaaaag aagccgccga acagggcaaa acgcctgaac
     1681 ggcattttgc ccacttaacc atacacggca cgctgcacct gatgggatac gaccacatca
     1741 aagacgatga ggccgaaata atggaagccg aagaaatccg cctgatgcgg gcggcaggct
     1801 atcccaaccc ctaccgagag gacggacatt aaaatggacg gcgcacaacc gaaaacaaat
     1861 ttttttgaac gcctgattgc ccgactcgcc cgcgaacccg attccgccga agacgtatta
     1921 aacctgcttc ggcaggcgca cgaacaggaa gtttttgatg ccgacacact gacccggctg
     1981 gaaaaagtat tggactttgc cgagctggaa gtgcgcgatg cgatgattac gcgcagccgc
     2041 atgaacgtat tgaaagaaaa cgacagcatc gaacgcatca ccgcctacgt catcgatacc
     2101 gcccattcgc gcttccccgt catcggcgaa gacaaagacg aagttttggg cattttgcac
     2161 gccaaagacc tgctcaaata tatgttcaac cccgagcagt tccacctgaa atccgtcttg
     2221 cgccctgccg ttttcgtgcc cgaaggcaaa tctttgaccg cccttttaaa agagttccgc
     2281 gaacagcgca accatatggc aatcgtcatc gacgaatacg gcggcacgtc gggtttggtc
     2341 acctttgaag acatcatcga gcaaatcgtc ggtgacatcg aagacgagtt tgacgaagac
     2401 gaaagcgccg acaacatcca ctccgtttcc gccgaacgct ggcgcatcca cgcggctacc
     2461 gaaatcgaag acatcaacgc ctttttcggt acggaatacg gcagcgaaga agccgacacc
     2521 atcggcggct tggtcattca ggaattggga cacctgcccg tgcgcggcga aaaagtcctt
     2581 atcggcggtt tgcagttcac cgtcgcccgc gccgacaacc gccgcctgca cacgctgatg
     2641 gcgacccgcg tgaagtaagc agagcctgcc cgcaccgccg tttctgcaca gtttaggatg
     2701 acggtacggt cgttttctgt ttcaatccgc cccatccgcc aaacataaaa atgccgtctg
     2761 aaaccggaaa acaggttcag acggcatttc gcttatccgc ttattttttg ctcaaagtca
     2821 gccccgtcca gccgaataac agggttaaag ccaaactcaa atagcagaaa aaggcataag
     2881 gcagatattc ccaaacgggt acgccaaggg cgtggctgat aaatacgccg cacacgctcc
     2941 acggcacgag cgggttaatc accgtccccg catcttccag agtccgcgac aggttgcgcg
     3001 aatgcaggcc gagtttgtcg taaacgggtt tgaacgtttc tcccgaaagc aggatgctca
     3061 aatattgctc tccaatcagg aaattgaccc cgaccgaagt catggcaacg ctgaacgtcg
     3121 cgcgtccggc attcgtcaag aaggtacgga cggcctccag cagggaagga atcacaccga
     3181 gcgcaaacag cagcccgccc aaactcatac cgaggataac gatggtctgc gtaaagaaca
     3241 tactctccaa gccgccgcgc gaaatcagtt tggcaatgtc tttaaacgct tcgccttcga
     3301 gtttgtagcc gccataaaac cacgcgccga gctgacgcag atcgggcgtg ctgtgcagat
     3361 acgttacggc aacggcggca atgacggtaa agagcatggc gaccacggca ttgacgcgca
     3421 tcaatgccaa aacgaccaac agtgcaaacg gaatcagcga atagccgtgc accaatcccg
     3481 tggcttcaag ctggctgcgg aaggattcga cgctgttcaa atcctgcgcg gcgacgctgg
     3541 gaagaagcca aagcatcagt gccgcgctga taagccacgc ggggatggtg gtgtacatca
     3601 tgtttttgat gtgttcaaac aggtcgatac cgacgatgga cgcggaaatg cccgtggtgt
     3661 cggaaagcgg ggacatttta tcgccgaaaa acacaccgga aacaatcgcg cccgccgtca
     3721 tcgccatatc ggcctgaaac gccgccgcca tccccataaa ggcaacgccg gcagtggcgc
     3781 aggcggtcag gctgctgccg atggacacgc cgatgacgga acacagcgcg aaggcggaaa
     3841 aataaaaata agtcggggaa atcagcccga aaccgtaata catcagcgtc ggaatcgcgc
     3901 cgctcatcat cagcgcgctg accatcagcc cgatgaagaa aaacaggtaa accgcgccca
     3961 taccctgatt caacgcgcct atcatccctg cctgcatatc gttgtatttc aaaccgcgcg
     4021 ccaagccgta caaaatcagc acgacgatgg cggcaataat ggacatatgc ggcagccact
     4081 ccaatgaaat gatggtatag cccattgcgg caatcagagc gacgacgacg gcaagtgcct
     4141 caccgcgcgg catatcgagt aaggatttga aagcaaacat gattctttgc ccgatttgat
     4201 ataaagttaa cggattctaa cacaccatgc cgaatctgcc cgacgttttc agacggcatt
     4261 ttagcgtttt acaaaagccg ggcagaaaca aaacggcaat tttctcgatt taaattaatt
     4321 tttttgaaat ataattttta tttttttatt tgttggcata taagactttt atggattgaa
     4381 gtattaaaac ctcggcacac acgccgcaaa caccggtacg caaaaatgcc gtctgaaaga
     4441 tgttcagacg gcatttctca agaaaaagat tatttgtccg ccctatatcc gtacaccgca
     4501 aaaaacacga tatacaggta acagataacg gaaacgacaa acgaagacat cagggtgtaa
     4561 gtatccgccg cccaaccttg aattaccggc acgaccgcac cgccgacaat cgcggtacac
     4621 agcacaccgg aagccgcgtt ggtgaatttg cccaaccctt tggttgccaa agagaaaatc
     4681 gtcgggaaca taatcgagtt gaaaaaaccg atggcaagca gcgaccacat cgccacatcc
     4741 gcattgccgc tgcccgtcgc catcgcgacg gcaagcagca cgaccgcagc cgatgcgtta
     4801 aacgccaaat aacggttggg cgcgaatttc gccattaccg ccgaaccgag gaaacgtccg
     4861 accatcgctc cgccccaata aaacgacagg taatgcgcgg cagaagcatg atccagccct
     4921 ttcagataac ccaacacatt gaccatcagc gaaccgatag acacctccgc accgacgtag
     4981 cagaaaatgc ctgccgtacc gaacacgaga tgtttgtatt gccatacgcc ggttttgccg
     5041 tcgtggttgt gcgcgctttc ctcggcggca attttgcgcg cgtcgggcag ccggatcatt
     5101 ttcacgaaaa cggcaaggat aatcagcagc cccgccagtc ccaaataggg aatctgtacg
     5161 gaagaaatct gttccgcctt gctgacggtt tgggttgcgt ccgccaaaat caggaacgcg
     5221 ccgatttgag gcgcaatggt cgtacccaaa gcgttaaacg cctgaaccag cgtcagcgtt
     5281 gccgattcct tgccgggttt cgccagcagg gtaacataag gattaccggc gacctgaagc
     5341 agcgttacgc cggaagccaa aataaacaac gcgcccaaaa ataccgcgta agaatggctg
     5401 cccgcagcag gataaaacag caggcacccg accgccgtca gcagaaagcc gccgataacg
     5461 ccgtttttgt agccgacttt gccgacaaaa gcccccatcg ggatggacat taccgcatag
     5521 gcggtaaaaa agcaaaattg aatcagcatc gcctgaacgt aagagaggtc gaaaatttct
     5581 ttcaaatgag ggataaggat gtcgttcatg caggtaataa aacccatcat gaagaacagc
     5641 gtggtcaaga cgaccaatgg ggacgtatgg ttgttttgtg attgcgcaga catgatttgc
     5701 ctttcgtggt gtcggaatat tgcccgtttc aagaaaatag aaattgacat ttcttaacgg
     5761 caatgcaaat catactgata tttgaacatt ttgcaaactt tatcggatgc aggcaatgcg
     5821 ccgatctgaa attcaagcag cagaaattaa tattttacag ttgtttgcat aggttttaat
     5881 agattttaaa aaattccggc gggcttgcaa aaaccgttcc caataaaatg aaaatcctcc
     5941 gccgtatccg gaaaattcgg aaatgccgca tcggggcatc aaaaccggag cggaaagaat
     6001 gtcgtatgcc cgacaaataa aggggcatac ggctgtgccg tatttttgca ccgtcctccg
     6061 cccttcggcg ggggcgtgaa aataccgtat aatccgtcgc cgatttttac ctgtttggga
     6121 ctgtttgcaa tgataccctc ctacacctac gccgccctcg ctttttccgc cttcacttcc
     6181 gccaccctcc tgcccggcac gtccgaagcc gccttcgccc tgttcgtccg caacttcccc
     6241 aaacacgcat acggcgcgtt gctgtgcgcc ggccttgcca acggattggg cagtatggtt
     6301 tcctattgga tgggacgttt gctgccttcc cgaaaaatgc cgtctgaaaa aacactgaat
     6361 ctgatacggc gtttcggtat ttggctgctt gcgtttgcgt ggctgcccgt cgtcggcgac
     6421 gcactgccgc ttaccgccgg ctggctgcgg ttgaatccgt ggacaagcgg gctgatgctg
     6481 gttatcggca aaacggcgcg ttacgccttc attctgtggg gaatgcaata ttacgccgcc
     6541 tgaaccgtgc gttataatgc cgaccgcaca aaccgactgc acaaaaaaaa tgaacagaca
     6601 aatccgccaa gaaatcttcg agcgtttccg cgccgccaac ccccatccga ccaccgagct
     6661 gaatttcaac tcccctttcg agcttttaat cgccgttctg ctttcggcgc aggcgaccga
     6721 tgtcggcgta aacaaggcga cggcgaagct gtttccggtt gccgatacgc cgcaggcgat
     6781 gctggatttg ggcttggacg gcgtgatgga atacacgaaa accatcgggc tgtataaaac
     6841 caagtccaaa cacattatgc aaacctgccg catcctgctg gaaaaataca acggcgaagt
     6901 gccggaagac cgcgaggctt tggaatcgtt gcctggcgtg gggcgcaaaa cggcaaacgt
     6961 ggtattgaac acagcgttcg gacaccccgt tatggcggtc gatacgcata ttttccgcgt
     7021 gtccaaccga accaaaatcg cacccggaaa agatgtacgc gaagtcgaag acaaactgat
     7081 gcgcttcatt cctaaagaat ttctgatgga cgcgcaccac tggctgattt tgcacggacg
     7141 ctacacctgc aaggctttaa agccgcaatg ccaaacctgc atcatcaacg atttgtgcga
     7201 atatcccgcc aaatcctgac cgccgccgcc cttcaaaatg aaacgcttga aaattcggac
     7261 ggacgttgta gaatttcaac ctatgccgtc tgaagccgac aaaatgcgct tcagacggca
     7321 ttaaagccgg taccggcatc cgacaaagga caaaacgtgt tcaaaaaata ccaatacttc
     7381 gctttggcgg cactgtgtgc cgccttgctg gcaggctgcg aaaaggcagg cagctttttc
     7441 ggtgcggaca aaaaagaagc atccttcgta gaacgcatcg aacacaccaa agacgacggc
     7501 agtgtcagta tgctgctgcc cgactttgcc caactggttc aaagcgaagg cccggcagtc
     7561 gtcaatattc aggcagcccc cgccccgcgc acccaaaacg gcagcggcaa tgccgaaacc
     7621 gattccgacc cgcttgccga cagcgacccg ttctacgaat ttttcaaacg cctcgtcccg
     7681 aacatgcccg aaatccccca agaagaagca gatgacggcg gattgaactt cggttcgggc
     7741 ttcatcatca gcaaaaacgg ctacatcctg accaataccc acgtcgttgc cggtatgggc
     7801 agtatcaaag tcctgctcaa cgacaagcgc gaatataccg ccaaactcat cggttcggat
     7861 gtccaatccg atgtcgccct tctgaaaatc gacgcaacgg aagagctacc cgtcgtcaaa
     7921 atcggcaatc ccaaaaattt gaaaccgggc gaatgggtcg ctgccatcgg cgcgcccttc
     7981 ggctttgaca acagcgtgac cgccggcatc gtgtccgcca aaggcagaag cctgcccaac
     8041 gaaagctaca cacccttcat ccaaaccgac gttgccatca atccgggcaa ttccggcggc
     8101 ccgctgttca acttaaaagg acaggtcgtc ggcatcaatt cgcaaatata cagccgcagc
     8161 ggcggattca tgggcatctc ctttgccatc ccgattgacg ttgccatgaa tgtcgccgaa
     8221 cagctgaaaa acaccggcaa agtccaacgc ggacaactgg gcgtgattat tcaggaagta
     8281 tcctacggtt tggcacagtc gttcggtctg gataaagcca gcggcgcatt gattgccaaa
     8341 atccttcccg gcagccccgc agaacgtgcc ggcctgcagg cgggcgacat cgtcctcagc
     8401 ctcgacggcg gagaaatacg ttcttccggc gaccttcccg tcatggtcgg cgccattacg
     8461 ccgggaaaag aagtcagcct cggcgtatgg cgcaaaggcg aagaaatcac aatcaaagcc
     8521 aagctgggca acgccgccga gcataccggc gcatcatcca aaacagatga agccccctac
     8581 accgaacagc aatccggtac gttctcggtc gaatccgcag gcattaccct tcagacacat
     8641 accgacagca gcggcaaaca cctcgtcgtc gtacgggttt ccgacgcggc agaacgcgca
     8701 ggcttaaggc gcggcgacga aatcctcgcc gtcgggcaag tccccgtcaa tgacgaagcc
     8761 ggtttccgca aagctatgga caaggcaggc aaaaacgtcc ccctgctggt catgcgccgt
     8821 ggcaacacgc tgttcatcgc attaaacctg caataacgca cataatttaa ccaaaatatt
     8881 tttaaatgat aaaatgcccg tcaataccga ttaacgggca ttttacaaaa atcagttggt
     8941 tgattttaaa caaatatttt ttacgacaaa aaatatttcc acccattttg ccaacaaaac
     9001 ccaaaccgga ggcggacaga tacccaccca tccggacgct taacaccccc cataaaccat
     9061 gcagctgatt gactattcac attcattttt ctcggttgtg ccaccctttt tggcactggc
     9121 acttgccgtc attacccgcc gcgtactgct gtctttaggc atcggtattt tggtcggcgt
     9181 tgcctttttg gtcggcggca accccgtcga cggtctgaca cacctgaaag acatggtcgt
     9241 cggcttggct tgggcagacg gcgattggtc gctgggcaaa ccaaaaatct tggttttcct
     9301 gatacttttg ggcattttca cttcactgct gacctactcc ggcagcaatc aggcgtttgc
     9361 cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg aaaatgctga ccgcctgcct
     9421 cgtgttcgta acctttatcg acgactattt ccacagcctc gccgtcggtg cgattgcccg
     9481 ccccgttacc gacaagttta aagtttcccg cgccaaactc gcctacatcc tcgactccac
     9541 tgcctcgccc atgtgcgtgc tgatgcccgt ttcaagctgg ggcgcgtcga ttatcgccac
     9601 gcttgccgga ttgctcgtta cctacaaaat taccgaatac acgccgatgg ggacgtttgt
     9661 tgccatgagc ctgatgaact attacgcgct gtttgccctg attatggtat tcgtcgtcgc
     9721 atggttctcc ttcgacatcg gctcgatggc gcgtttcgaa caggctgcgt tgaacgaagc
     9781 ccacgacgaa accgccgctt cagacgctac caaaggtcgt gtttacgcat tgattattcc
     9841 cgttttggcc ttaatcgcct caacggtttc cgccatgatc tacaccggcg cgcaggcaag
     9901 cgaaaccttc agcattttgg gggcatttga aaataccgac gtaaacactt cgctggtatt
     9961 cggcggcact tgcggcgtgc ttgccgtcgt cctctgcacg ttcggcacga ttaaaaccgc
    10021 cgattatccc aaagccgtgt ggcagggtgc gaaatccatg ttcggcgcaa tcgccatttt
    10081 aatcctcgcc tggctcatca gtacggttgt cggcgaaatg cacacgggcg actacctctc
    10141 cacgctggtt gcgggcaaca tccatcccgg cttcctgccc gtcatcctct tcctgctcgc
    10201 cagcgtgatg gcgtttgcca caggcacaag ctgggggacg ttcggcatta tgctgccgat
    10261 tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt atcccgtgta tgtccgcagt
    10321 aatggcgggg gcggtatgcg gcgaccactg ttcgcccatc tccgacacga ccatcctgtc
    10381 gtccaccggc gcgcgctgca accacatcga ccacgttacc tcgcaactgc cttatgccct
    10441 gacggttgcc gccgccgccg catcgggcta cctcgcattg ggtctgacaa aatccgcgct
    10501 gttgggcttt agcacgaccg gtattgtatt ggcggtgctg atttttctgt tgaaagataa
    10561 aaaacgcgcc gacgtttgac cttcccccct gttcagacgg catatgccgt ctgaagtttt
    10621 ttggagcaaa ccgcaatgac cgtcccccat attccgcgcg gccccgtcat ggcagacatt
    10681 gccgccttcc gcctgaccga agaagaaaaa caacgcctgc tcgatcccgc aatcggcggc
    10741 atcatcctct tccgccgcaa cttccaaaac atcgaacaac tcaaaacact caccgccgaa
    10801 atcaaagccc tgcgcacacc cgaactcatt atcgccgtcg atcacgaagg cggcagggtg
    10861 caacgtttca tcgaaggttt tacccgcctg cccgcaatga gtacgctcgg cgagatttgg
    10921 gacagtgaag gcgcggaaac tgccgaaact catgccgaac acatcggatg ggttttggca
    10981 accgagcttt ccgcctgcgg catcgatttg tccttcacgc ccgtcttaga tttggactgg
    11041 ggaaactgcg ccgtcatcgg caaccgcagc ttccaccgca atcccgaagc ggtcgcccgc
    11101 ctcgccctcg cccttcaaaa aggtttggca aaaggcggta tgaaatcatg cggcaaacat
    11161 ttccccggac acggatttgt cgaaggggac agccatttgg tgctacccga agacgggcgc
    11221 agcctcgacg aactcgaagc cgccgacctc gcccccttcc gcattatgag ccgcgaaggt
    11281 atggcggcgg tcatgcccgc ccacgtcgtt tatccgcaag tggacacaaa gcccgcaggg
    11341 ttttccgaaa tctggctcaa acaaattttg cgccgcgata tcggattcaa aggcgtgatt
    11401 ttttctgacg atttgaccat ggagggcgcg tgcggggcag gcggcatcaa agaacgcgcc
    11461 cgcatttcct ttgaggcagg ttgcgacatc atattggtct gcaaccgtcc cgacttggtc
    11521 gatgaattgc gcgacggttt taccattccc gacaaccagg atttggccgg acgttggcaa
    11581 tacatggaaa actcgctcgg tcatgaggcg gtacaagccg ttatacagac aacgggtttc
    11641 caagcggcgc aagcctttgt tgccggcctt gcctcgccgc aggacacggc gggcggcgta
    11701 aaagtcggag aggcctttta aaccggctgc gtttcaacaa tctgccgatt tcaaccgttc
    11761 gtcagggaaa ggcaatgatt ttttcggtca aatagaaagt tatcttattg aattactttg
    11821 agataaggat gcttatcaat actgtattca aacacagccc tttaaatccg cccgacacgc
    11881 ggcaaaaagg caaaacggta taggcacgcc gtattttttg cgctataatc acaccttaat
    11941 taattcggaa tgtagcgcag cccggtagcg cacttcgttc gggacgaagg ggtcggaggt
    12001 tcgaatcctc tcattccgac catattcaga ccgcctcgac atcgattgcc ggggcggttt
    12061 ttcccttttg gcggcaacaa aaaaatgccg tctgaaacag aatgctgttt cagacggcat
    12121 ttcgttttcc ccaatcctgc catttgcgcg cactgacaaa gggtatcggc aaggttcaaa
    12181 agaccatcgg aatctaggaa gcctggcgtg tacccaaccg cggcatctta ttcattaagg
    12241 cttcgtcctg attgggcggc ggaaatgtcg gcagtttcac gcggaagcgg cacactttcc
    12301 ccgtatgtca aacatcggca agaaggtacg cctgcccttt gggttacact gcttaaacta
    12361 ctctatcccc gcccatttgt gcgtctgcac gctcaactgc caatgcacgg gcgcgtcggg
    12421 gcggctgttt aaaataccga tttggcggat ggtgtcgtag atgttcatca cgccgttttg
    12481 ttcgcaggga gaaagatagt aatggcgcgc acggattttg cgttccatat tttcgcaaaa
    12541 cgcaaggaca tcgccgtcgg caacaatccg tacttcgtcg gcttcggcga tgcagttggt
    12601 ttcatacttg gcggcgtagc aggctttggg gctggtggcg acatagtcga tttgcggcgg
    12661 cgcgggcttg agcccgttgg tttcgagaca gaggaaatag ccttccgcct tgagcgtgtc
    12721 cagcagcata tcgagatgcg gctggatggt cggctctccg ccggtgatga tgatgttgcg
    12781 ggcggcataa gttttcagac agcctaagat gtcggacagg ctcatcatac caaatgtcaa
    12841 ataatcggta tcacaccagc cgcacgccag attgcatttg cccaagcgga cgaaaacggc
    12901 aggcataccc gtgtttcggc cctcaccttg caggctttca aagatttcga cgatgcggta
    12961 ctggggattt tcgggggcga cgctgatttt tttcataccg ccaacaccgc cgccgccaaa
    13021 aatgcgggca aaccttgttt gacgagtatg cctttgttgc cggacgaaaa cgcaccccac
    13081 gcggcggcaa tcaggacgaa gccgagaaac agtaccgtcg cgccgtacac ggcattatcc
    13141 ggagcggcaa actgcgccca caaaagcccg acggcaagaa agccgttgta cagcccttgg
    13201 ttgccaaaca gggtctgcac gcgtttctgt tccataaatt cgtaaggcag cttgaacatt
    13261 tccgccgctt tttcgccggg aatctgcgtc atttcaagcc aggcgatgta gaaatgttcg
    13321 gcggcgacga ggaggacgag aatggtggag aggagtttca tgggtgttcc tttcaatggg
    13381 tgtgccggta tgaaatgccg tctgaaaaaa cggggaatcc gtgcaggaga aaaaaccgct
    13441 ttacg
//
LOCUS       DAWTCI010000057        13388 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWTCI010000057 DAWTCI010000000
VERSION     DAWTCI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.057"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(244..1094)
                     /locus_tag="WM481_001555"
                     /pseudo
     CDS             complement(244..1094)
                     /locus_tag="WM481_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            1444..2958
                     /gene="katA"
                     /locus_tag="WM481_001556"
     CDS             1444..2958
                     /gene="katA"
                     /locus_tag="WM481_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFA9080844.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLTQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPASYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRSQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFDNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            3474..4040
                     /locus_tag="WM481_001557"
                     /pseudo
     CDS             3474..4040
                     /locus_tag="WM481_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
     gene            4262..5425
                     /locus_tag="WM481_001558"
     CDS             4262..5425
                     /locus_tag="WM481_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HFA9080845.1"
                     /translation="MSFKLRYLASVLALSSLLAACGGQEKSAAGDASPASETEAASQV
                     QASEAVPSASSASPEDQDLLKRAQGVFQPLPTVEEMQKIRPFTEEQVKLGHQLWYEPR
                     LSKGNTVSCNSCHNLASAGVDNMPTSQGHKGQFGGRNSPTALNAALLGSQFWDGRAAD
                     VEEQAGGPLVNPVEMANDSQEAAAAKIAKVPEYQEMFKKAFPEDGAVSFKNITTALGA
                     FERTLLTPTKWDEYLKGNVNALSEQERKGVRAFMDNGCIACHNGVNLGGTTFQKFGLV
                     QGPYWKFIEDPKRDKGRADVTKKTEDEFFFRVPGLRNVAKTYPYFHNGSVWELDKAVT
                     IMGKAQLGKDIPKEDVDNIVVFLNALSGNVSESARTMPELPLTAPMESKPDNK"
     gene            complement(6006..8042)
                     /locus_tag="WM481_001559"
     CDS             complement(6006..8042)
                     /locus_tag="WM481_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFA9080846.1"
                     /translation="MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVVDTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFATLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYAGNRELREQIYRAYVTRASELSNDGKFDNTANIDRTLENALKTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLS
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFAEKTVPVWHKDVRYF
                     ELEQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     GKEARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMTIYSEDNEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8130..9002)
                     /locus_tag="WM481_001560"
     CDS             complement(8130..9002)
                     /locus_tag="WM481_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9080847.1"
                     /translation="MEIWNMLNTWPDAVPIRAEAAESVAAVAALLLARALLLNIHFRR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RYLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNHPAGSETL"
     gene            9268..11658
                     /gene="gyrB"
                     /locus_tag="WM481_001561"
     CDS             9268..11658
                     /gene="gyrB"
                     /locus_tag="WM481_001561"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFA9080848.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKI
                     VGDSDKKGTTVRFLAGTETFGNIEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVINQALTDFLEENPNEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVATLITALGAGIGKEEFNPEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEAALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGLVGEGAKLYKGENEYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            11757..13142
                     /locus_tag="WM481_001562"
     CDS             11757..13142
                     /locus_tag="WM481_001562"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFA9080849.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQTARIAIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLGRIRTQHILRLNGQEIRFIP
                     DRDLNIRGNQVLPKHPNGLRFTAYASDGTVLKEQVYYSVGGGFVVTEEDFEWQSETEK
                     AVPYPYNSCAGLLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVAEIMEECIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(13213..13311)
                     /locus_tag="WM481_001563"
     CDS             complement(13213..13311)
                     /locus_tag="WM481_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HFA9080850.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         3109 a         3645 c         3687 g         2947 t
ORIGIN      
        1 gccaccaacc tcaccggctc aaaccggcac agcaacttat atcccgtcat tctcgcgcag
       61 gcgggaatcc agccccgccg gtacagaaac ttatcgaaaa aaagtttctt cagattttac
      121 gttctagatt cccactttcg cgggaatgac gggatgcagg tttccgcgcg gacggattcg
      181 gcattcccgc tttcgcagaa atgtcggctt ttggtttgtt gtctttataa gattattcgg
      241 taattattct gcggttttgc gtttgtgtgc tgccggaatc tcaagggatt ctctgtattt
      301 ggcaacggtg cggcgggaaa cctctatacc gcggaaagcc agcaggttgg cgagtgcctc
      361 gtcagaatag gacttgggcg tggacgaggc aaaaccggcg gcgggaaagg ggttgagcga
      421 agcaatgagg ccgagtgcgg cttcgagtgt gccgctgtcg gtttggggca ggcgtttttt
      481 attcgcgccg gggtttggct gcggttgccg tcaatgctgt cgagggcgtt tcggacgata
      541 tgcagggcgg cgggtttggc agcacattcg cccgatcttt ctatctgcaa tatcaacgat
      601 tcggtcacat cggcggcggc catgcctgcc ggatcgaatt ttttcaatgc ggtcagggct
      661 tgtttcagca ttgcttcatc caacatccac tccaagggcg tatggtccag gatgtcttcg
      721 atgctgtcgg tcaggtagcc ctgctcgtca aggaaatcga taaggatgtg gacgcaggcg
      781 gcttcttggt cggaaagggg gtgttcgcac gcttgcgcgt gcaggtattg cttgaaatct
      841 tcttcgccgg cgatgttgga cagcatatct tcgccttcgt ctccgccgat ttgacgcgcg
      901 ggcgcggtgt aatggctgaa ttcggcatcg gaaaattcat ccgtttcttt gcgttcgagc
      961 agggggttgt ccgacggcca gttttcgacc tcgcgttcaa gttcgatacc cggcatctgc
     1021 aatacgcgca aagattgttg cagccgctgg tcgagctgct gggtctgttt gagctttatt
     1081 ccgattaagg taatgataat gcggtaaaaa ttgttatttt cagcctgtcg gcgcaaaaaa
     1141 atgccgcaaa gcgtcattgc attataaatg gttttaatga gcgggttcgg attccgtccg
     1201 ataacaaaaa acaaacgaaa atggaaactt gattgcttat aacaatatta aatcaatttc
     1261 atagttttaa taacgaaaat cttggcgtat aatcgcatcc atagtttttg caaaaggaaa
     1321 ataaaatgtc gattcaagaa atttattaca atcaagaaac cggttacgaa tacgctttcc
     1381 gccaaatcgt actgtaagcc gtttgccccc catttgacca acctgagaaa aggaacaaga
     1441 gcgatgacta cctccaaatg ccccgtaacc catctgacca tgaacaacgg cgcgcccgtt
     1501 gccgacaatc aaaacagcct gaccgccggc ccgcgcggcc ccctgctgac gcaagatttg
     1561 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1621 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacac acgacatcac caaatacacc
     1681 cgcgccaaaa ttttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1741 gtggcaggcg agcgcggcgc agccgatgcg gaacgcgaca tccgcggttt tgcgttgaaa
     1801 ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1861 cgcgacccgc gcaaattccc cgacctgaac aaagccgtca aacgcgatcc gcgcaccaat
     1921 atgcgttccg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc cctgcaccaa
     1981 gttaccatcg tgatgagcga ccgcggtatt cccgccagct accgccatat gcacggcttc
     2041 ggttcgcata cctacagctt ctggaacgaa gcgggcgaac gtttttgggt gaaattccat
     2101 ttccgcagcc agcaagggat taaaaacctg accaacgaag aagctgcaaa aatcatcgcc
     2161 gacgaccgcg aaagccatca gcgcgacctg tacgaagcca tcgagcgcgg cgagttcccg
     2221 aaatggacga tgtacatcca agtcatgcct gaagccgatg ccgaaaaagt gccttatcat
     2281 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2341 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2401 ccgagcaacc tcgttcccgg tatcggcgcg agcccggata aaatgttgca agcccgtttg
     2461 ttcaattacg ccgacgcgca acgttaccgc ttgggtgtga acttccgcca aatccccgtc
     2521 aatcgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggccg cgccgacggc
     2581 aactacggca gcctgccgca ctacgaaccc aacagcttcg gccaatggca gcaacaaccc
     2641 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cacactggga ctaccgccaa
     2701 gacgatgacg actacttcag ccaaccgcgc gccttgttca acctgatgaa cgacgcgcag
     2761 aaacaggctt tgtttgacaa caccgccgca gccatgggcg atgcgcccga cttcatcaaa
     2821 taccgccata tccgcaactg ctaccgttgc gacccggcat acggtgaagg cgtagccaaa
     2881 gccctcggac tgaccgtcga agacgcacaa gccgcccgcg cgaccgatcc cgcactgggt
     2941 caggccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     3001 gccgtccgat accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgt
     3061 ttcagacggc atttccacac ctcgccccgc cgtttcagtg ggcgtttttt attatagtgg
     3121 attaaattta aaccggtacg gcgttgcctc gccttgtcct gatttttgtt aatccactat
     3181 aaacgcaaaa tatcccgtca ttcccgcacc atccccaccg gttcaaaccg gcacgaaaac
     3241 ttttccgcgt cattcccgcg aaagtgggaa tccggaacgt aaaatctaaa gaaaccgttt
     3301 tatccgataa gtttccgtgc cgacggggct ggattcccac tttcgtggga atgacgggat
     3361 ttctgttttt gattttttgt ttttgcggga ataacgggat gcgggttttc gtgcggcgtt
     3421 tttcgcgctt tgctgttttg ttataatccg ccctttttaa ggatgggtgc ggtatgagtc
     3481 tttacgcttt gctcttggtt gcgctgggaa tgtcgatgga tgcgtttgcc gtcgcgctgg
     3541 caaagggtgc ggcggtcagg atgcccccgc gcaaaattgc agcaacggct ttggtgttcg
     3601 gtacggttga agcgttcatg ccgctggcgg gctgggtagg cggtttttat gccaagccgt
     3661 ttatcagcga atgagaccat tgggtggctt ttgtcctgtt gggcggactg ggtctgaaaa
     3721 tgatgcgcga agggttgtcc ggcgaggcgg aagatgtgcg cgaaagcaaa caggaaagcc
     3781 tatggatgac ggttttgact gcttttggaa ccagtattga ttccatgatc gtcggggtgg
     3841 gcttggcgtt tatggaggta aacatcgcct ttgccgccgc cgtaatcggt atggcggcta
     3901 cggtaatggt taccatcggg ctgacggcgg gaaaggcttt tggcgtattg ttcggcaggc
     3961 gtgcggaatt tgccggaggt ttggtgttga ttgccatcgg tacatggacg ctcttatcgc
     4021 atttgggttt gattcaatga tgtcggaaaa taaaatgccg tctgaagctt taccggtttc
     4081 agacggcatt ttgttaaact tgattctcta tcggtattat tgtgtaaagc tattgaaaat
     4141 aaaatattga ttttccgcaa agatggctaa aggtgcgata ggtacactga agaaataatc
     4201 ggcagaacag gtttgcctgc cggtttgaac aataatttta acctcccata aggaatacac
     4261 gatgtctttc aaactccgtt acctcgcatc agtattggca ttgtcttcac tgttggcggc
     4321 atgcggcggt caggaaaagt ctgcggcagg cgatgcttct cctgcttccg agaccgaagc
     4381 ggcttcccaa gtacaggcct cggaagccgt tccttccgct tcatcggcct cgcccgaaga
     4441 ccaagacctt ttgaaacgcg cgcaaggtgt attccaacct ttgccgactg tcgaagaaat
     4501 gcagaaaatc cgtccgttta ccgaagaaca ggtcaaactc ggacaccaac tctggtatga
     4561 accacgcctt tccaaaggca ataccgtaag ctgcaactct tgccacaacc ttgcttccgc
     4621 cggtgtggac aatatgccga ccagtcaggg gcacaaaggg cagttcggcg gacgcaactc
     4681 gcctaccgca ttgaatgctg cgctgctggg cagccagttt tgggacggac gtgccgccga
     4741 tgttgaagaa caggctggcg ggcctttggt gaatccggtg gaaatggcga atgattcgca
     4801 agaggcggct gcagccaaaa tcgccaaagt tcccgaatat caagaaatgt ttaaaaaagc
     4861 ttttcctgaa gacggcgcgg tttcgtttaa aaacatcact accgcattgg gtgcgtttga
     4921 gcgtaccctg ctgacgccga ccaaatggga cgaatacctc aaaggcaacg tcaacgccct
     4981 gagcgaacag gaacgaaaag gcgtgcgcgc gttcatggac aacggctgta ttgcctgcca
     5041 caacggtgtc aaccttggag gcacgacctt ccagaaattc ggtctggtcc aagggccgta
     5101 ttggaaattc attgaagatc cgaaacgcga caaaggccgt gctgacgtaa ccaaaaaaac
     5161 cgaagacgaa tttttcttcc gtgttccggg gttgcgtaac gtggctaaaa cttatccgta
     5221 tttccacaac ggcagcgtgt gggagctgga taaggcagtt accatcatgg gtaaggcgca
     5281 attgggtaaa gacattccga aagaagatgt ggataacatc gtcgtattcc tgaatgcact
     5341 ttccggcaat gtttccgaat cagcgcgcac gatgccggaa ctgcccctga ccgcaccgat
     5401 ggaatctaag ccggacaaca aataatccgg ccggtacgca aaaatgccgt ctgaaacggg
     5461 aatctgtttc agacggcatt ttatatttcg tcgcgtcaga tacgcagggt atgccgtagg
     5521 gtgtgcttca gccagccgtt tccaccaatt caaccaacca tgtccgggtc ttatgcgttt
     5581 ttcacgaatg cgcggcgtag gcggagggat tggtgggctg aagcctaatt caatggctgc
     5641 gttgtgtaag ccatgatgcg tttggataat taatcaatat tcttgcgcta ccattaccat
     5701 cggctttggg ggtttgtact tcggcattat gataatcaat agcctgtttg gattgtattc
     5761 ccgaatccga cagttttaga ttataaaaag gccgtctgaa aaattttcag acggcctttt
     5821 atagtaaatt tcaaataaaa atatctttgt cgtcattccc acgcaggcgg gaatccagac
     5881 ttgtcggcac agaaacttat cgggtaaaac ggtttcttca attttatgtt ccggattccc
     5941 gcctgcgcgg ggataacgat tcaggtattt ctacatcgaa tccgcttttt atatcaacct
     6001 gactatcaag ccgcgttgtc gaagccgctg tggcgcagca gtgcgtctat gctcggttcg
     6061 cgtccgcgga aggccttgaa ggattccgcc gcgctgcggg agccgccgac ggcgaggatt
     6121 tcctgccaga agcgtttgcc tgtggcggca acgtcgtcgc tttcttcaaa ggcggcgtag
     6181 gcgtcggcgc tgaggacttc cgcccatgcg tagctgtaat agcctgcgga gtagccgccg
     6241 gcgaagatgt ggccgaagct gttggcgaag cggttgtatt cgggcggttg gatgacggcg
     6301 acttctttgc gcacgctgtc taaaacctgc tgccagtttt tcagacggcc ttcgttgtct
     6361 tcgctgtaaa tggtcatgtc aaagagggcg aattccattt ggcggacgag gaacatgccg
     6421 cgttggaagt ttttggcggc gagcattttg tcgaagagtt ctttcggcag gggctcgccg
     6481 gtttcttcgt gggcggacat ttgtgccaat acattgtatt cccaaacgaa gttttccata
     6541 aactggctgg gcagttcgac cgcgtcccat tctacgccgt tgatgccgga cacgcccagt
     6601 tcgtccactt gggtaagcag gtggtgcagt ccgtggccgg tttcgtggaa gagggtgagg
     6661 atttcgtcgt ggcttaaacg cgcttctttg ccgccgacgg gcggggcgaa gttgcagacg
     6721 aggtaggcgg tgggcagttg cagcgtgccg tcggcaaagc ggcggcggcc tttgtagtcg
     6781 ttcatccacg cgccgccgcg tttgccttcg cgtgcgtaca aatccatata aacgccgccg
     6841 atggttttgc cgttttgctc cagctcgaag tagcgcacgt ctttgtgcca gacggggacg
     6901 gttttttcgg caaatccgat gccgtagagt tttttgattt gggcgaacag tccgtttaat
     6961 actttgccga cggggaagta ttttttgact tcggtttcgc tgaatgcgta tttggcttcg
     7021 cgcagttttt cgccggcgta gctcaagtcc cacggctgcg ggtcggcgag accgaggtgt
     7081 tcgcgggcga aggctttgac ttcggcgagg tctttttcgg cgtagggttt ggcgcggcgg
     7141 gcgaggtcgt gcaggaagtt taaaacctgt tcgggcgtgt ccgccatttt ggttgccagc
     7201 gacaattcgg cgtaattttt aaagccgagc agtttggcgg ttttcaatgc gttttcgagc
     7261 gtgcggtcga tgttggcggt gttgtcgaat ttgccgtcgt ttgaaagttc gctggcacgg
     7321 gtaacgtagg cgcggtagat ttgttcgcgc agttcgcggt tgccggcgta ttggatgacg
     7381 gcaaggtagt gcggaatctg caagccgatt ttgtaacctg ttttgccttc gctttgcgcg
     7441 gcggcggcaa acatcgcgag cgcgtcttcg ggaatgccgg caagcggtgc ggcatcgtca
     7501 aagtaaatgc cgaacgcgtc ggtcgcgtct aggacgtttt gggagaattt ggcggaaagt
     7561 tgcgcgcctt cggtttgcag ttttgccagt tctgcctgcc gttcgggcgg cagttccgcg
     7621 ccgctcaata cgaaatcgcg caggtcgtga tcgagcttgg ttttttgtgc gggggaaagc
     7681 gttgcaaatt cgggggaatt tttgatggtt ttgaagcggt tgtacagttc gatgtcttgt
     7741 ccgatttcgg tgaagaagac ggtgatttca ggcatcagtt cgttatagac ggcgcgcagt
     7801 tcgggcgtgt cgacgacgga gttgagatgg gacacgacgc cccaaatcct gccgacgcgt
     7861 tcggtgatgc cggtcagacg ctcgacggtg ttcgcccagc cggtgtgcgt ttgcgctttg
     7921 acggcggcga tttgtccgcg cgcttcggcg atggcggttt ggacggcggg tttgatgtct
     7981 tcggttttga tttgattaaa acggggttct tcgcccaagt ggagcagtgc gttgtcaatc
     8041 ataagatggg tttcctttgc gtgttgtgct tcagacggca tatcggcaat atgccgtctg
     8101 aagccagaaa tggggccggc cggggaaagt taaagtgttt cggagccggc gggatgattt
     8161 aacaggcggt attgtacgcg caaaaattcg tccataaccg cctgttggat ttccagccgc
     8221 tttgaaacgg gggaggcgaa gcggacgatg atgcggtatg ccttgtcgtc gtacggtacg
     8281 cgggtaacgc gcggcctggc ggcgggcgtg ataaacagtt tttccgcctg cacgttttcc
     8341 aaataccgct gaatggcggg gatgtagggc gcgcacaagg gctcgagtac ggctttcaga
     8401 cggcatacgg cttcatccga atccaaatgg atgggaacgg ggatttcgac cgtatggatg
     8461 acatagtcgc ccaaaatatt gtcgcggcgc acggggtggc tcaacaacag gctgttgggg
     8521 aaagaaacgg tggttcccgc aagctgtccg accaaggggt tcggaccgac ctgcatcatc
     8581 agcgtgttca acagattgat gtcgaccacg cgcccgcgca ggccgttgat ttcgatatag
     8641 tcgccgaccg agtattgctg ggtggcagac cttaaaatac tgcccgacag acacataatc
     8701 agttcttttg tcgccacgac gaccgccgcc gccaccgcaa acatcgacaa agccagcgtt
     8761 tgaatttgcg ccgaccagat aaatgccagc gaaaacagca ccaaaagcag cgttatattg
     8821 cggctggcaa ccaaaaaccg ccgcttgctt tcgatgccga aatccggatg ccgtctgaag
     8881 tggatattca acagaagggc gcgcgccagc agcaaagccg cgaccgccgc cacggattcg
     8941 gccgcctccg cgcgtatcgg gacggcatcg ggccaagtgt tcaacatatt ccatatttcc
     9001 atttccgcgc ccctgtccga aaagtatagg gtgtagattt tagtggcaaa aaaacgcttt
     9061 tgccactttg ccggcaaatt ccgcgcccgt accggccgca aatgcgccgc acgccctttc
     9121 agacggcatc cggatgcgga taacgctgaa tcaggcggcg aaatcggcta taatacacaa
     9181 ttatatgcgc cgtcccgccc gctttttcca caaggtgcgg actgttttgg cggcatcgca
     9241 aaatcttttc aaaatccggc aaaaaatatg actgaacaaa aacacgaaga atacggcgcc
     9301 gacagcatcc aggtgctcga aggcttggaa gcggtacgca aacgccccgg catgtacatc
     9361 ggcgacacgc aggacggcag cgggctgcac catatggtgt ttgaagtatt ggacaacgcc
     9421 atcgacgaag cactcgccgg acattgcgac aaaatcacgg taacgataca cgccgaccat
     9481 tccgtcagcg tcgccgacaa cgggcgcggt atgcccaccg gcatccaccc gaaagaaggg
     9541 cgttccgccg ccgaagtcat catgaccgtc ttgcacgcgg gcggcaaatt cgacaacaac
     9601 agctacaaaa tctccggcgg cctgcacggc gtgggcgtat ccgtcgtcaa cgcgctgtcc
     9661 gactgggtaa cgctgaccat ctaccgcgac ggcaaagaac acttcgtccg cttcgtacgc
     9721 ggcgaaaccg aagagccgct gaaaattgtc ggcgattccg acaaaaaagg cacgaccgtg
     9781 cgcttcctcg ccggcacgga aaccttcggc aatatcgaat acagcttcga catcctcgcc
     9841 aaacgtattc gcgaactttc gttcctaaac aacggcgtgg acatcgaatt gaccgacgag
     9901 cgcgacggca agcacgaaag cttcgccctt tccggcggcg tggcgggctt cgtgcaatac
     9961 atgaaccgca aaaaaacgcc cttgcacgaa aaaatcttct atgcgttcgg cgagaaagac
    10021 ggcatgagcg tcgaatgcgc aatgcaatgg aacgacagct atcaggaaag cgtgcagtgc
    10081 ttcaccaaca acatccctca gcgcgacggc ggtacgcacc tgaccgcgct gcgccaagtg
    10141 atgacgcgca ccatcaacag ctacatcgaa gctaacgaag tcgccaaaaa agccaaagtg
    10201 gaaaccgccg gcgacgatat gcgcgaaggt ttgacctgcg tgttgtccgt caaactgccc
    10261 gaccccaaat tctcatccca aaccaaagac aaactggttt ccggcgaaat cggccccgtt
    10321 gtcaacgaag tcatcaacca agcactaacc gacttcctcg aagaaaatcc gaacgaagcc
    10381 aaaatcatca ccggcaaaat cgtcgatgcc gcccgcgcac gcgaagccgc ccgcaaagcc
    10441 cgcgaaatca cccgccgcaa aggcgtgatg gacggcttgg gactgcccgg caaactcgcc
    10501 gactgccaag aaaaagaccc tgccctgtct gaactctacc tcgtcgaggg cgactccgca
    10561 ggcggttccg ccatgcaggg ccgcgaccgc aaattccaag cgattttgcc gctcaaaggt
    10621 aaaattttga acgtcgaaaa agcacgtttt gaaaaaatgc tcgccagcca agaggtcgcc
    10681 accctgatta ccgcgctggg tgcaggcatc ggcaaagaag agttcaaccc tgaaaaacta
    10741 cgctaccacc gcatcatcat catgaccgat gccgacgtgg acggtgcgca catccgcacc
    10801 ctgctcctga ccttcttcta ccgccaaatg cccgaactgg tcgagcgcgg ctacatttac
    10861 atcgcccagc cgccgctcta caaagccaaa tacggcaagc aggagcgtta cctcaaagac
    10921 gaactggaaa aagaccaatg gctgctcggc cttgccttgg aaaaagccaa aatcgtttca
    10981 gacggccgca ccatcgaagg cgcagaactt gccgacaccg ccaaacaatt cttgttggcg
    11041 aaaaccgtca tcgaacagga aagccgcttc gtggacgaac tcgtcctgcg tgccatgctg
    11101 cacgcgtcgc ccattgattt gacgtcgtct gaaaacgccg ataaagccgt tgccgaactt
    11161 tccggtttgc ttgacgaaaa agaagccgcc ctcgaacgca tcgaaggtca tgaaggacac
    11221 cggttcatca aaatcacgcg caagctgcac ggcaacgtca tggtcagcta catcgaaccc
    11281 aagttcctca acagcaaagc ctaccaaacc ctcacccaaa ccgccgccgc gctcaaaggc
    11341 ttggtcggcg agggcgccaa gctctacaaa ggcgagaacg agtacgacgc ggacagcttt
    11401 gaaaccgctt tggacatctt gatgagcgtt gcccaaaaag gtatgtccat ccaacgatac
    11461 aaaggtttgg gcgagatgaa ccccgagcag ctttgggaaa ccacgatgga tcccgccgtg
    11521 cgccgcctgt tgaaagtgcg catcgaagat gccattgccg ccgacgaagt gttcgttacc
    11581 ctgatgggcg acgaggtcga accgcgccgc gcctttatcg aaaacaatgc gctgattgcg
    11641 caaaatatcg acgcataagt gccgttttaa aaaaggagac gggcagcttg ccgcgtctcc
    11701 ttttggtttg tcaaacggaa gccatgccgt ctgaaaccgc ctttcggagc aaaaccatga
    11761 tcagcatttt tgatattttc aaaatcggta tcggcccttc cagttcgcat acggtcggcc
    11821 cgatgaaggc cgccgccgcc tttgccgcag gcttggacgc gcaaaccgcc cgcatcgcca
    11881 tcgacattta cggctcgctt gccctgaccg gatacggaca cggcacattt gacgcgctga
    11941 tgctcggttt ggaaggcagc ctgccgcacg acatcccgct tgccggcatt cccgaacgcc
    12001 tcggacgcat ccgcacgcag cacatcctcc ggctcaacgg gcaagaaatc cgcttcatcc
    12061 ccgaccgcga cctgaacata cgcggcaatc aagtgctgcc caaacacccc aacggcctgc
    12121 gatttaccgc ctatgcttca gacggcacgg tattgaagga acaggtttat tattcggtcg
    12181 gcggcggctt tgtcgttacc gaagaagact tcgagtggca gtcggaaacg gaaaaagccg
    12241 ttccttatcc ttacaacagt tgtgccgggc tgcttgcccg atgccgtctg aaccggctcg
    12301 acatctccga agtcgtgttg gcaaacgaag ccgcgcttgc cggatgcggc gaagccgaaa
    12361 tccgccgccg ggttgccggt gttgccgaga ttatggaaga gtgcatcaaa cgcggcttgg
    12421 gtgcggacgg cgaactgccc ggcggattga acgtccgccg ccgcgccccg cagcttgccg
    12481 ccaaactcaa agtcctgcgc gaaaccgaaa tcgtcaacac ccagctctgg ccgatggtgt
    12541 acgccatggc ggtcaacgaa gaaaacgccg ccggcggacg cgtcgttacc gccccgacca
    12601 acggcgcggc aggcatcatt cccgccgtat tgcattattt ccgcaagttc aacccccacg
    12661 ccacacagga acgtgtcgaa aacttcctgc tcaccgcagg cgcaatcggc atcctctaca
    12721 aaaccaacgc ctccatttcc ggcgcggatg tcggttgtca gggcgaagtc ggcgtagcgt
    12781 gttcaatggc ggcgggtgca tacgccgaag tcatcggcgg cacgcccaaa caagtggaaa
    12841 acgccgccga aatggcaatg gaacaccatt tgggcttaac ttgcgacccc gtcggcgggt
    12901 tggtgcaaat cccctgcatc gaacgcaacg gcatcgccgc cgaaaaagcc ctcaaactcg
    12961 gcacgctcgc gctcttggaa gacggcacgg acaaaaaagt ctcgctcgac gaagtcatcc
    13021 gaaccatgct gcaaaccggc cgggacatga aggcgaccta caaagaaacc tcgcttgccg
    13081 gactcgccgc cacgctccgg aaaaaagccg tccccgtatc cgtgcgcgtg gtcgagtgct
    13141 gaggatggac agaaacgaaa atgccgtctg aaaacagttt ttcagacggc atttcattta
    13201 tttcttgcgg gtttaccgct tcaacgccaa cgtcaacacg cccgccgtaa ccagccccaa
    13261 gcctatccat tcctgcgtgt tcgggcgttc gtccaagaaa accaccgcca tcaggaatgc
    13321 aaaccggcgt tgtttgcagc atccggcaag aatttttata gtggattaac aaaaatcagg
    13381 acaaggcg
//
LOCUS       DAWTCI010000058        13183 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWTCI010000058 DAWTCI010000000
VERSION     DAWTCI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.058"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(201..686)
                     /gene="purE"
                     /locus_tag="WM481_001564"
     CDS             complement(201..686)
                     /gene="purE"
                     /locus_tag="WM481_001564"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFA9080851.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFEYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAISMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(734..1447)
                     /locus_tag="WM481_001565"
     CDS             complement(734..1447)
                     /locus_tag="WM481_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFA9080852.1"
                     /translation="MKTKLPLFIIWLSVSASCASVLPVPEGSRTEMPTQENASDGIPY
                     PVPTLQDRLDYLEGKIVRLSNEVEMLNGKVKALEHTKIHPSGRTYVQKLDDRKLKEHY
                     LNTEGGSASAHTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGSIAQRSMYLL
                     LQSRARMGNCESVIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLI
                     QTYPGSPAAKRAAAAVRKR"
     gene            complement(1447..2115)
                     /locus_tag="WM481_001566"
     CDS             complement(1447..2115)
                     /locus_tag="WM481_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFA9080853.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKAGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAAFDAPPSVKILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(2126..2311)
                     /locus_tag="WM481_001567"
     CDS             complement(2126..2311)
                     /locus_tag="WM481_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFA9080854.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELAQERLKNRGLLWDVELNPEEQK
                     NFESAKVAKKPYTYYQD"
     gene            2455..4431
                     /gene="mutL"
                     /locus_tag="WM481_001568"
     CDS             2455..4431
                     /gene="mutL"
                     /locus_tag="WM481_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFA9080855.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAVD
                     VELEGGGIRLIRVGDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNSALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLELPPKAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRANLTE
                     SVSNAGEVLHDITGVTPAPMPSENDSENLFDSASNHPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMDTYAELYKKTDDIDLELSQFEQARFGNMPSETPAHKT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQHLLIPVTFAASHEECAALADHAETLAGFGLELSDMGGNTLAVRAAPVML
                     GKSDVVSLARDVLGELAQVGSSQTIASHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDTLFLRGQ"
     gene            4526..6649
                     /gene="dnaX"
                     /locus_tag="WM481_001569"
     CDS             4526..6649
                     /gene="dnaX"
                     /locus_tag="WM481_001569"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFA9080856.1"
                     /translation="MTYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYDPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIVNQDGEALLAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEADAAQTPVQTASAAAMPSEGKTAGPVSHQENNDVPPWEDAPDKTETAAGTA
                     RTSAKSIQTASEAETPPENQVSKNKAADNETEASLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGFPDNDCPPEDGVEIPPPDWANVLPADTAGGGTDEEAEAGGIGGNNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYRSDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTEDWRDEAGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5715..5754
                     /gene="dnaX"
                     /locus_tag="WM481_001569"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6730..7065
                     /locus_tag="WM481_001570"
     CDS             6730..7065
                     /locus_tag="WM481_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFA9080857.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQDL
                     PPGVGDFFR"
     gene            complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WM481_001571"
     CDS             complement(7718..8764)
                     /gene="recA"
                     /locus_tag="WM481_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFA9080858.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEISDEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8964..9728
                     /locus_tag="WM481_001572"
     CDS             8964..9728
                     /locus_tag="WM481_001572"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFA9080859.1"
                     /translation="MHTSLTVKNTVIGSGRTKIAVPLVARDAADLSSVLSQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQAVRDALPDKPLLFTFRRHCEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSSEDVLTLLSATLEAKRLVAKPVITMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9748..11763
                     /gene="rep"
                     /locus_tag="WM481_001573"
     CDS             9748..11763
                     /gene="rep"
                     /locus_tag="WM481_001573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFA9080860.1"
                     /translation="MMKLNPQQLEAVRYLGGPLFVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKPQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNVWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTRYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGNYRAKAEIDEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVGEL
                     VSWFARKGERDGKNVIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRIDNLKKSGPAD"
     gene            complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WM481_001574"
     CDS             complement(11833..12891)
                     /gene="dinB"
                     /locus_tag="WM481_001574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFA9080861.1"
                     /translation="MSLRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATVKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     PDEAYLDVTRNFKNIPYAGEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGAGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDEHPVKAERERLQISTEITLPEDLPLGQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGRLVPKNQQQDLWA"
BASE COUNT         3222 a         3841 c         3419 g         2701 t
ORIGIN      
        1 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat atttcaaaaa
       61 ttgaaacccg cacagattgt taaaccccaa aacaggggcg ggcgaaattc ttccggcaat
      121 gggttcatgc aaacaaaatg ccatctgaac cccgaaaaca gggcttcaga cggcatttgt
      181 gtattcgcgg acggcgggtt ttaaatctgt tccaattcca tgtttaaaac ggtttgttcc
      241 tgtttggctc ggaagtctgc cagtttttgc gccagttcgg ggttttcgtt ggcgagcatg
      301 gagatggcga acaatgcggc atttgccgcg cctgcctcgc cgatggcgaa tgtggcgacg
      361 ggtacgcctt tgggcatttg tacaatcgat aaaagcgaat cttcgccgcg caggtatttg
      421 cttgggacgg gtacgcccaa aacggggacg gtggtcttgg cggcaaccat gccgggtaaa
      481 tgcgccgcgc cgcccgcacc cgcgatgatg gctttgatgc cgcgcgcccg tgcggtttcg
      541 gcgtattcaa acatcaaatc cggagtgcgg tgtgcggaaa cgacgcgcgc ctcatattct
      601 acgccgaact cttcaagaaa ctgcgctgcc tgccgcataa cgggccaatc gctgttgctg
      661 cccatgatga tgccgatttg tatcataaat ccttcttggt gcggatgggg taaaaagcgg
      721 aaaaatggaa aaactatcgt ttgcgtacgg ctgcggcggc gcgttttgcc gccgggctgc
      781 cgggataggt ctgtatcagg ctgcgccaag tcgcccttgc aatgtctttt tgctgaagcc
      841 tgtattggca ttcgccgatt ttgaatatga cttcgggcgc ggttgggctg tctttgaaac
      901 ggttggcgta acgccctccg atttcgatga cagattcaca gttccccata cgcgccctgc
      961 tttgcagcaa caggtacata ctgcgttgcg cgatgctgcc gccgtctccg ccgtccgccc
     1021 ccttcaacaa ggcggctgcg gcagaaaacc tgccgttttg atagtgtttg agtgcctgat
     1081 tgtagaggtt ttgtgcggtt tcgacggtat gtgcggatgc gctgccgcct tcggtattga
     1141 ggtaatgctc tttcaatttg cggtcgtcga gtttttggac gtatgtcctg ccggaagggt
     1201 gtattttcgt atgctccaat gctttgactt tcccgtttaa catttccact tcgttcgaca
     1261 gccggacgat tttgccttcc agatagtcca aacggtcttg cagagtggga acgggatagg
     1321 gaatgccgtc tgaagcattt tcctgtgtcg gcatttcggt tcggctgccc tccggaacgg
     1381 gtaaaacgga agcacaggag gcagacacag acagccaaat gataaaaagc ggtaatttgg
     1441 tcttcattat tttttcagaa gcagggtcaa accgtcgccg acgggcaggg tgatggggac
     1501 aatccgcgta tcgttcggca ggttttgatt gaaatctttg agaattttga cactgggcgg
     1561 cgcatcaaaa gccgcttcgc gcatcaccct tccgttcaac aaaatattgt cgatggcgat
     1621 gatgccgcct tgccggacga gtttgaggca acgctcgaaa tattgcggcg tgggcggttt
     1681 gtctgcgtct atcagtgcca aatcgtagct tccggcttca ccctgtgcaa tcaaatcatc
     1741 caatgtcagc aatgcgggtt gcaggtgcag gctgatttta tgcgccacac cggcctcgtt
     1801 ccaaacctga cgcgccgtat cggtaaaggt tacattgatg tcgcaggcgg taatccgccc
     1861 gtgttcgggc agcgccaatg caagcgcggt gctgctgtat ccggtaaata cgccgatttc
     1921 cagatatttt tccgcgcgaa tcagctttgc cagccaaacc aaaaccgccg cctgttcgcg
     1981 cgcaatcgcc attttgccca tacggtgatg cccggccttc tcacgcagcc gcgtcaaaac
     2041 gggatgttcg ggttcgccga tggcattcaa atagttttgc aggtccggtg cgacattgga
     2101 cagatgggtc gtcatttagg cgggttcagt cttggtaata ggtataaggt tttttcgcca
     2161 cttttgccga ttcaaagttt ttttgctctt cgggattgag ttcgacatcc cacaaaagcc
     2221 ctcgattttt caaacgttct tgcgccaatt caggtttttc ttcgatcagg cggttgagga
     2281 attgtgtggc ttcggattgg tagtggtaca tctttgcgct ccaattttac gggatacggc
     2341 gtgattatac tggtattttc caaacgggat aaacggcttt tatcaagaat acgggcagaa
     2401 agataagggg ttttattata gaataaggcg ttttttgcaa cggaagaccg ccttatgcca
     2461 cgcattgccg ccctgcccga ccacctcgtc aaccaaattg ccgccggcga agtggtcgaa
     2521 cgccctgcca acgccctgaa agaaatcgtt gaaaacagca tagacgcggg cgcaacggcg
     2581 gttgatgtgg aactggaagg cggcggcatc cgcctcatcc gcgtcggcga caacggcggc
     2641 ggcatccacc ccgacgacat cgaactcgcg ctccaccgcc acgccaccag caaaatcaaa
     2701 accctgaacg atttggaaca cgttgccagc atgggctttc gcggcgaagg cttggccagt
     2761 atcgcctccg tcagccgcct gaccctgacc agccgccaag aagacagttc gcatgcgacc
     2821 caagtcaaag ccgaagacgg caaactcagc agccccaccg ccgccgccca ccccgtcggc
     2881 accaccatcg aagccgccga actcttcttc aacacccccg cgcggcgcaa gttcctcaaa
     2941 tccgaaaaca ccgaatacgc tcactgcgcc accatgctcg aacgcctcgc gctggcgcat
     3001 ccgcacattg ccttctcact caaacgcgac ggcaaacaag tgttcaaact tcctgcccaa
     3061 agcctgcatg aacgtatcgc cgccattgtc ggcgatgatt ttcagacggc atctttggaa
     3121 attgacagcg gcaatagcgc gctgcggctc tatggcgcga ttgccaagcc gactttcgcc
     3181 aaaggcaaaa ccgataaaca atattgcttc gtcaaccatc gttttgtccg cgacaaagtc
     3241 atgctccatg ccgtcaagca ggcataccgc gacgtattgc acaacgccct cacccctgct
     3301 ttcgtccttt tcctcgagct gccgcccaaa gccgtggatg tcaacgtcca cccgaccaaa
     3361 accgaaatcc gcttccgcga cagccggcaa gtacaccaac tcgtgttcca cacgctcaac
     3421 aaagcccttg ccgacacacg cgccaacctg accgaaagcg tcagcaacgc aggcgaagtg
     3481 ttgcatgaca ttaccggcgt tacgcctgcc ccaatgccgt ctgaaaacga cagcgaaaat
     3541 ctgtttgata gcgcatccaa ccacccgaca ggcaacaaac ccgatacacg caatgccttt
     3601 ggttcatcag gtaaaaccgc gcccatgcct taccaggccg cgcgtgcgcc gcaacaacac
     3661 agcctgtccc tgcgcgaaag ccgcgcggca atggacacct atgccgaact ctacaaaaaa
     3721 accgacgaca tcgaccttga gttgagccaa ttcgaacagg cacgtttcgg caatatgccg
     3781 tctgaaacgc ctgctcacaa aacagatacg ccgctttcag acggcatccc gtcccaatcc
     3841 gaactgccgc cgctcggttt cgccattgcc caattacttg gcatctacat tcttgcccaa
     3901 gccgaagaca gcctgttgct catcgatatg cacgccgctg ccgaacgcgt caactacgag
     3961 aaaatgaaac gccaacgtca ggaaaacggc aacctgcaaa gccagcacct gcttattccc
     4021 gtaacctttg ccgcgtccca cgaagaatgc gccgccctcg ccgatcatgc cgaaacgctg
     4081 gcaggcttcg gcctggaact gtccgacatg ggcggcaaca ccctcgccgt ccgcgccgcg
     4141 cccgtaatgt tgggcaaatc cgatgtcgtc tccctcgcac gcgacgtatt gggcgaactc
     4201 gcccaagtcg gcagcagcca aaccatcgca tcacacgaaa accgcatcct cgccacaatg
     4261 tcctgccacg gctcaatccg cgccggccgc aggctcaccc tgcccgaaat gaacgcgctg
     4321 ctgcgcgata tggaaaatac gccgcgcagc aaccagtgca accacggcag gccgacgtgg
     4381 gtcaaactga ctttgaaaga attggacaca ctgttcttgc gcggacagta agccgaaaat
     4441 gctagaatac gccgcccgaa gccgccgttc agacggcatt ccgacgcacc gacagaaaca
     4501 tcacgaccga aaccaagaga aaaacatgac ctatcaagtt ctcgcccgaa aatggcggcc
     4561 caaaaccttt tccgacttag tcggtcagga acacgtcgtc aaagccctgc aaaatgcctt
     4621 ggacgaaggc aggctgcacc acgcctacct gctgaccggg acgcgcggcg tgggcaaaac
     4681 caccatcgcc cgcatccttg ccaaaagcct caactgcgaa aatgcgcaac acggcgaacc
     4741 ttgcggcgtg tgccaaagct gcacccaaat cgatgccgga cgctatgtcg acctgctcga
     4801 aatcgacgcc gcctccaaca caggcatcga caacatccgc gaagtcttgg aaaacgccca
     4861 atacgcgccg accgccggca aatacaaagt ctatatcatc gacgaagtgc atatgctttc
     4921 caaaagcgcg ttcaacgcca tgctcaaaac gctggaagag ccgcccgaac acgtcaaatt
     4981 catcctcgcc accaccgatc cgcacaaagt ccccgttacc gtattaagcc gctgcctgca
     5041 attcgtctta cgcaatatga ccgcgcaaca ggttgccgac caccttgccc acgtcctcga
     5101 cagcgaaaaa atcgcctacg acccccccgc cctgcaactt ttgggacgtg ccgccgccgg
     5161 atcgatgcgc gatgccttaa gcctgctcga ccaagccatc gccctaggtt cgggcaaagt
     5221 tgccgaaaac gatgtccgcc aaatgatcgg cgcggttgac aaacaatacc tgtacgaact
     5281 gctgacaggc atcgtcaacc aagacggcga agccctgctg gcaaaagcgc aggaaatggc
     5341 ggcgtgtgcc gtcggctttg acaacgcctt gggcgaactg gccatactgc tgcaacaact
     5401 cgccctgata caggcagtcc cctccgcctt ggcgcacgac gaccccgatt ccgatatttt
     5461 gcaccgcctc gcccaaacca taagcggcga acaaatccag ctttactacc aaatcgccgt
     5521 ccacggcaaa cgcgacctgg gcctcgcccc cgacgaatac gccggcttta tgatgaccct
     5581 gctgcgtatg ctggcgttcg cccccttggc ggcggcttcg tgtgatgcaa acgccgtgat
     5641 tgaaaatacc gaacttcaat ccccgtcggc acaaaccgcc gaaaaggaaa ccgccgcaaa
     5701 aaagccacaa ccgcgccctg aagcggatgc cgcccaaacg cccgttcaga cggcatccgc
     5761 agcagcaatg ccgtctgaag gcaaaactgc cggccccgtt tcccatcaag aaaacaacga
     5821 tgttccgcct tgggaagacg cgccggacaa aaccgaaacc gcagccggca cggcgcgaac
     5881 gtcggcaaaa agcattcaga cggcatccga agccgaaacg ccgcccgaaa accaagtttc
     5941 caagaacaag gcagccgaca acgaaaccga ggcttccttg tccgaagtgc cgtctgaaaa
     6001 ccccattcag gcaacaccga atgatgaagc cgttgaaacg gaaacatttg cacacgaagc
     6061 ccctgcagaa cctttctacg gttttccgga taatgactgc cccccggaag acggcgtgga
     6121 aatcccgccg cccgattggg caaatgtcct ccctgccgat accgcaggcg gagggacgga
     6181 cgaagaagca gaagcgggcg gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga
     6241 attttccacc gaaaactggg cagccatcgt ccggcacttc gcccgcaaac tcggcgcggc
     6301 gcaaatgccg gcgcaacact ccgcgtggac ggaataccgt tccgacaccg gactgatggt
     6361 tttggcaatg accgccgaag cccgcgccac cgccgacaaa aagcggctcg acaaaatccg
     6421 cgacaccctc gcccaagcct acgggctgca actcaccctg caaaccgaag actggcgcga
     6481 cgaagccggc cgggaaaccc ccgcgatgca ggacaagcgt gtccaagccg aagacaggca
     6541 aaaagcacaa gcattgctcg aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg
     6601 cgcgcaatgg cagcccgaat cactggaatt ggcggcaaac cggccataaa cagatataat
     6661 gccgcccgaa cccttcggac ggcattgccg cttccctcat tcattcaaac acaaacagga
     6721 gtattcagta tgttcggaaa agccggatta ggcggcctga tgaaacaggc gcagcaaatg
     6781 caggaaaaca tgaaaaaggc acaagccaaa cttgccgaaa ccgaaatcga aggcgaagca
     6841 ggcaacggct tggtcaaaat cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc
     6901 agccccgatt tgattcaaga agccgccgat gacaaagaaa tgctcgaaga cctcatcctc
     6961 gccgccctca aatccgcccg gggcaaagcc gaagaaaccg caaacaaaac aatgggcgca
     7021 ttcacccaag atctgccccc cggcgtgggc gacttcttcc gctgaccccg accgtcattc
     7081 ccgcgaacgc gggaatccgg taaccgaaaa accacaggaa tctatcggaa aaaaacagaa
     7141 acccccgccg ccgtcattcc cgcgcaggcg ggaatctaga acgtagaatc tgaaaaaccg
     7201 ttttactcga taaatttccg tgccgacagg tctggattcc cgccttcgcg ggaatgacgg
     7261 catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag tgcaaagtat
     7321 tgcacataaa agggcgcagg cttcatccgt aaagtcggcg caggatagag gcaaagcggg
     7381 cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggtaaaaata tagtggatta
     7441 acaaaaaccg gtacggcgtt gcctcgcctt agatcaaaga gaacgattct ctaagatgct
     7501 gaagcaccaa gtgaatcggt tccgtactat agtggattaa atttaaacca gtacggcgtt
     7561 gcctcgcctt gccgtactat ttgtactgtc tgcggctctc cgccttgtcc tgatttttgt
     7621 taatccgcta taccaaaact caaatcaagc cgtccggagg cggctcaaaa aaaacggtac
     7681 ttcgcagcag aagtaccgtt tatcgggatt tcaggtttta ttcttcgggt cgttcgccgt
     7741 cggtttcgtc ctgcgtccct tcggtgatgt gcatttctac gccgttgagg gcgcggattt
     7801 ttgcgtcgat ttcgtcggaa atttcggggt tttctttcag ccagacgcgg acgttgtctt
     7861 tgccctgacc gattttcgcg ccgttgtagc tgtaccacgc gccggatttg ttgatgatgt
     7921 cgtttttcac gccgatgtcg atcaattcgc cttcccaact gatgccttct ccgtagagga
     7981 tgtcaaactc tgcctgacgg aacggggggg cgactttgtt tttgatgact ttgacgcggg
     8041 tttcgttgcc caatacttct tcgccttttt tgatggatcc ggtgcggcgg atgtcgaggc
     8101 ggacggaaga atagaatttc agcgcgttgc cgccggtcgt ggtctcgggg ctgccgaaca
     8161 ttacgccgat cttcattcgg atttggttga tgaacacaac cagcgtattg gtttttttga
     8221 tgtgtccggt cagtttgcgc aaagcctgac tcatcaggcg cgcctgcagt ccgacgtggc
     8281 tgtcccccat atcgccttcg atttcggctt tggggacgag tgcggctacg gaatcgacga
     8341 ctaccatatc tatgccgccc gaacggacga gcgtgtcgca gatttccaaa gcctgttcgc
     8401 cggtatcggg ctgggacagg taaagctctt cgacttttac gccgagtttg cgggcgtaaa
     8461 cgggatcgaa ggcgtgttcg gcatcgacaa aggcgcacac gccgccgttt ttctggcatt
     8521 gggcgacggc ttcgaggcag agtgtggttt tgccggagga ttcggggccg aagatttcga
     8581 cgatgcgccc gcgcggcagg ccgccgactc cgagggcgag gtctaatccg agcgatccgg
     8641 tggaaatgac ttcgaggttt tcttcctgct ggctgccgtc cattttcatg atggcgcctt
     8701 tgccgaaact tttttcgatt tgcgccagtg cggcggcaag ggctttgctt ttgtcgtctg
     8761 acatgggggt tactccggaa caaatgcggt atgtgggctg cggcgcaaca cgggctgcgg
     8821 cgcggaatat gaatcggtcg gtcattatcg catgtttgcc tttcgggggc taccatccgc
     8881 ttcagaccga atgccgtctg aaacattgtc cctcctttgc tataatggcg cccttacttt
     8941 tcccgccagc cgcttgaacc gccatgcaca ccagcctgac cgtcaaaaat accgttatcg
     9001 gaagcgggcg caccaaaatc gccgtgccgc ttgtcgcccg agatgccgcc gatttatcct
     9061 ccgtgctgtc ccaaatcaaa aacctgccct tcgatatcgt cgagttccgc gccgactttt
     9121 tggaatgcgc gggcagcatc ggcgaagtat tgcgccacac gcaggccgtc cgcgacgcgc
     9181 tgcccgacaa accgctgctg tttacgttca gacggcattg cgaaggcggc tcgttcccgt
     9241 gttcggacga ttattatttt gaactgctcg acgcgctgat cgaaagccgc ctgcccgaca
     9301 ttatcgatat cgagctgttt tccggcgaaa ccgccgtccg ccgcgccgtg gcaaatgctc
     9361 aaaaaaacgg catcgccgcc ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag
     9421 aagaaatcgt atgccgtctg aaacagatgg aggactgcgg cgcggacatc tgcaaaattg
     9481 cggtgatgcc gcaaagctcg gaagatgtgc tgactttgct ttccgccacg ctcgaagcga
     9541 aacggcttgt cgccaaaccg gttattacga tgtcgatggg gcagacgggt gcggtcagcc
     9601 ggcttgccgg acaggtgttc ggctcaagca tcacgttcgg ttcgggaacg caaaactccg
     9661 cgccggggca aatcggcgta tccgccctcc gtgcggcact cgactgcctc gaaagcggcg
     9721 cagactgatt tcagacggca tcaaaacatg atgaaactca atccccaaca gctcgaagcc
     9781 gtccgctacc tcggcggccc cctgttcgtc cttgccggcg caggcagcgg caaaaccggc
     9841 gtgattacgc aaaaaatcaa gcatttgatt gtcaatgtcg gctatctgcc gcataccgtc
     9901 gccgcgatta cctttaccaa caaagccgct gcggaaatgc aggagcgcgt cgccaaaatg
     9961 ctgcccaaac cgcaaacgcg cgggctgacg atttgcacgt tccattcttt gggcatgaag
    10021 attctgcgcg aagaggcgaa ccatatcggt tacaaaaaaa acttctccat tctcgactct
    10081 accgacagcg ccaaaatcat cggcgaactc ttaggcggca cgggaaaaga agccgtattc
    10141 aaggcgcagc accagatttc cctttggaaa aacgatttaa aaacgcctga agatgtcgtt
    10201 cagacggcct ctaatgtgtg ggaacaacaa accgcgcgcg tgtatgcaag ttatcaggaa
    10261 accctgcaaa gctatcaggc agtagatttt gacgacttaa tccgcctgcc tgccgtgctg
    10321 ttgcagcaaa acagtgaagt gcgcaacaaa tggcagcggc ggctgcgtta tctgttggtt
    10381 gacgaatgcc aagatacgaa tacctgccaa tttgcgctga tgaaactctt gacaggcgcg
    10441 gaagggatgt ttaccgccgt cggcgacgac gaccagtcca tctacgcatg gcgcggcgcg
    10501 aacatggaaa acctgcgcaa aatgcaggaa aactatccgc agatgaaggt catcaaactg
    10561 gagcaaaact accgctccac cgcgcggatt ctcaaaattg ccaacaaagt catcgaaaac
    10621 aaccccaagc tgtttaccaa aaaactttgg tcgcaattgg gcgaaggcga gccggttaaa
    10681 gtcgttgcct gccaaaacga gcagcacgaa gccgattggg tcgtcagcca aatcgtcaaa
    10741 caaaaactca tcggcggcga caaaacccga tacgccgatt ttgccgtgtt ataccgcggc
    10801 aagcatcagg cacggatttt cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc
    10861 tccggcggac aaagcttttt cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg
    10921 ctgcttgcca accccaacga cgatcccgcc tttctgcgcg ccgttaccac gccaaaacgc
    10981 ggcatcggcg atgtaacgct gggcaaactc aacgcctacg cgcacgagca cgaatgcagc
    11041 ctgtatgaag ccgcacaaaa cgaagaagcc cttgccacac tgaacaatac caaccgccaa
    11101 cacctgcaag cctttatgga tatgttcggc aactaccgcg ccaaagccga aatcgacgaa
    11161 gcgggcgagt tcatcaacag cctgctcgaa gaaatcgact atgaaaacca tttgatgcaa
    11221 aacgaagaag gcaaagccgg cgaaatcaaa tggcgcaacg tcggcgagtt ggtatcgtgg
    11281 ttcgcacgaa aaggcgaacg ggacggcaaa aacgtcatcg aactcgccca aaccgtcgcc
    11341 ttgatgacgc ttttggaagg aaaagacgaa gaagaaaccg atgccgtctc gctatccacg
    11401 ctgcacgccg ccaaaggttt ggagtatccg tatgttttcc ttgtcggttg cgaagaaggt
    11461 gttttgccgc acaacgacag catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg
    11521 tacgtcggca tcacccgtgc caaacgccaa ctcacgctga cccactgtgt caaacgcaaa
    11581 aaacaaggca tatggcaatt ccccgaaccc agccgattca tagacgaaat gccgcaggaa
    11641 gatttgaaaa tcctggggcg caaaggcggc gaaccgattg tcagcaaaga agaaggcaga
    11701 cgtaaccttg ccgatataat cggaaggatc gacaacctaa aaaaaagcgg cccggcggat
    11761 taaaccggag ccgcaatgcc gtctgaaaga cgttcagacg gcatattttt tgaacggcgc
    11821 gcgtcaagcg gtttacgccc acaaatcctg ctgctgattt ttcggtacaa gcctgccgac
    11881 accgataccg atgaggcgga acgcgtcttc cgtctgcggc gggacgcgcg ccatcaacat
    11941 ttgcgcagcc tgcagcagag ctgcgcagtc gggcaatacg gaggaataag tcagcgtgcg
    12001 cgtgatgatg cggaaatcgt aggtcttcag cttgagcgtt acgctttggg cttcgacgtt
    12061 tttgcgcgtg atttgccgcc acaggtcttc ggcaagatgg ggcaggtgtc cggcagcctg
    12121 cccgagcggc aggtcttcgg gcagggtgat ttctgtggaa atttggaggc gttcgcgttc
    12181 ggctttgacg gggtgttcgt ccgtaccgcg cgccaaatca tagaggcggt agccgtagcg
    12241 tccgaaatga tttaagagct cgccgcgctc gaaacggcgc aaatcgcccg ccgtccgcat
    12301 acccagcgac tgcatttttt tcagcgttac cttgcccgct ccggggattt tgcccaaagg
    12361 cagggtttcc aaaaatgcca taactttgtg cggcggcaac acaaactgcc cgttcggctt
    12421 gcgccagtcg gaagcaattt tcgccagaaa tttgttcggc gcgatgcctg cggatgcggt
    12481 caaacccgtt tccgcaaaaa tggcggcacg gatttctttg gcaacttcgc cggcgtaagg
    12541 gatatttttg aaattacggg taacgtcaag ataggcttcg tccggcgaca agggttcgat
    12601 taaatcggta taacgcctga atacggcgtg aatctgcgcg gaaacctgac ggtacaaatc
    12661 gaaatgcgga ggcacataca ccgcttgcgg acacagcctt ttcaccgttg ccaccgacat
    12721 cgcggaatgc agcccgaact gccgtgcctc atacgatgcg gcgcaaatca ccgaacgcgc
    12781 gccctcccac gcgacgacca ccggccgccc tttcaaatgc ggctgctcgc gtagttctac
    12841 cgatgcgtag aacgcgtcca tgtcgatgtg gatgattttg cgtaaagaca tcggctctgc
    12901 cgaggataaa agggatattt tactgcgggc atcggacaaa atccaaatat actaacaggt
    12961 taaatacaaa agagttggct ggattaattt tgaaactcga taaaaataat ggtttttatc
    13021 aagcagaaag aaacagtaat ggaaattatg atatcgtaag aaaaaattat ctgaaaaata
    13081 ttaccaaaat acaatcaaat atattgattt acttagataa taccagtacc ggatttaaaa
    13141 ttcaacagag gagaaaacaa atcagagcac aaatttcagc cag
//
LOCUS       DAWTCI010000059        12995 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWTCI010000059 DAWTCI010000000
VERSION     DAWTCI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12995
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.059"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(106..1233)
                     /locus_tag="WM481_001575"
     CDS             complement(106..1233)
                     /locus_tag="WM481_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9080862.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKALKKISPDLVVPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRITLPMLESLIVLNPDD
                     KDELLHQYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDE
                     FIRAAEQVKGKYPDTVFTALGAIDKPRGSGDLEQPAARDIIRFPGFVNNVSEMIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENRAAVRLMGNASYAIAKDKFDAEKVDLKFLDILKA"
     gene            complement(1587..2812)
                     /locus_tag="WM481_001576"
                     /pseudo
     CDS             complement(1587..2812)
                     /locus_tag="WM481_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3205..4452)
                     /gene="fabF"
                     /locus_tag="WM481_001577"
     CDS             complement(3205..4452)
                     /gene="fabF"
                     /locus_tag="WM481_001577"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFA9080863.1"
                     /translation="MSQRRVVITGLGQVSPVGNTAAEAWDNLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEATGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHACKTVISST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(4609..4845)
                     /gene="acpP"
                     /locus_tag="WM481_001578"
     CDS             complement(4609..4845)
                     /gene="acpP"
                     /locus_tag="WM481_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFA9080864.1"
                     /translation="MSNIEQQVKKIIAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5077..6084
                     /locus_tag="WM481_001579"
     CDS             5077..6084
                     /locus_tag="WM481_001579"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFA9080865.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNTVYKLGLIPVTDNRTKPI
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFLEIGTVTRNPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDAMIRNIEKSKYQGVLGINIGKNAVTPIQNAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGGELGALLEALKNKQAQLAAAHGKYIPLAVKIAPD
                     LDEAQIEDIARVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGFPVREKSNRVL
                     KKLAEHIDGALPIIGVGGIMEGGDAAEKIRLGTTAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6281..6754
                     /locus_tag="WM481_001580"
     CDS             6281..6754
                     /locus_tag="WM481_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFA9080866.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLTNKSNIFELKPVLEDLASETRGYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENIARLYSHWEHYLNNGGGQG"
     gene            6768..7103
                     /locus_tag="WM481_001581"
     CDS             6768..7103
                     /locus_tag="WM481_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080867.1"
                     /translation="MPSEALSGVQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WGSVGKAAKAVGEGLIEAGNQHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKGAAY
                     AVLKERGEV"
     gene            7297..9987
                     /gene="glnE"
                     /locus_tag="WM481_001582"
     CDS             7297..9987
                     /gene="glnE"
                     /locus_tag="WM481_001582"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFA9080868.1"
                     /translation="MSDHRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKALTDEDFQ
                     AFADWDKIRAEESEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYQDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNGITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKSLVRPFVFRKYLDYGAYEAMRNLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKIAEMGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPISPEQRQLLAESMGFDSYAAFSDGL
                     NVHRNKVNQLFNEILSEPEEQAQSNSEWQWAWQEKPDEEERLGRLKEHGFDAEAVTAR
                     LEQICHGHKYRRLSAHAQPRFDTIVPLFVQAAAEQNNPTDTLMRLLDFLENISLRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DGIEACGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVIGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNRLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFGKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRLYRRQ
                     QHNTKLRDAAKTEVSDELLSHYGNVRKLWREVFGEEAATA"
     gene            10411..12618
                     /gene="uvrD"
                     /locus_tag="WM481_001583"
     CDS             10411..12618
                     /gene="uvrD"
                     /locus_tag="WM481_001583"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFA9080869.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRFGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLGAPDPHTSRMIGCYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRRHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMHLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIASRFVEEIPPEVLHYLSVKKPAFDSYGNTRQTTVQRDKIIDDFKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
BASE COUNT         3094 a         3594 c         3506 g         2801 t
ORIGIN      
        1 aaactcaacg aacctggatt cccgctttcg cgggaatgac ggggtttcgc gggaatggcg
       61 ggagtttgtc agaaatcacc gaaactcaaa agccgaccac cttgtttacg ccttcaaaat
      121 atcgagaaat ttcaaatcga ctttttcggc atcgaattta tctttggcaa tcgcataact
      181 tgcattcccc atcaggcgga cggcagccct gttttcgata aaataaatca ttttttcggc
      241 caagatgcgg ggattccaag gttcgatcag gaagccgttg accttgtcgg caaccgtttc
      301 cctgcatccg gggacatccg tcgtaatcac cgccctgccg acggccattg cctcctgagt
      361 gctccgggga acgccttccc tataataaga cggcaatacg aatatatgat gttcttttat
      421 catttcggaa acattgttca caaaaccggg gaaacggata atatcgcggg cggcaggctg
      481 ttccaaatcc ccgctcccgc gtggtttgtc gattgcgccc aaagcggtaa aaaccgtatc
      541 ggggtatttg cccttaacct gttccgccgc ccgaataaac tcatcaatcc ctttttcttt
      601 cagaaatctg ccgataaaga ggaattttac gggttctttt tcatcgggaa tatccgcctc
      661 ggaataagga tattgccgca aatccagacc gattccgccc aaaatatgga tgttttttat
      721 tttgatgccg tattgatgca gcaactcgtc tttgtcgtcg gggtttaata caatcaggct
      781 ttccaacatc ggcagggtaa tgcggtacaa ggcgattaaa atccccttta tgatttttgt
      841 ttttaacggt atgccttccg gctgcggggt aaatgcgaat cccaaacctt ccagcatccc
      901 gacgattctg ggcacgcctg ccaattttgc ggcaaaagtg ccgaaaatca cgggttttgc
      961 gaaataaggg acaaccaaat ccggcgatat ttttttgagt gctttaaata tgaggaaggt
     1021 ggattttata tcggaaaacg ggtttagccc gctgcggttt gaccggtagg taacgggtgt
     1081 aacccccatt tccctgataa tgtccaactc attgtcggaa aactccgata caaaggcata
     1141 cacctgatgg tttttgccga ttaatttttt aatgacgggg gcgcggaaac cgtaaatgct
     1201 ggatgcgact gttgtgataa aaacgatttt cataaggcgg acaccttgaa tataggattg
     1261 gaaatgcggt ctgcattgat gaaatgccgg atacggggcg cggcgggcaa acctttgcca
     1321 agcgcgccgc atccgacgct tcgcccgccc gggcaggggc ggcttcagac ggcattttat
     1381 cctgaaatcc gctgcataac gcccagcgat atacagtatc gcgttttgtg atgattttac
     1441 acccgaaggg aaaacgggct tatttgaatg gcggtttaat cgcggcggtt atttgcggtt
     1501 tgataaggct gatttgcgtg ccgtgcagtc cggcaacggg cggggtgttt ggaaaatgcc
     1561 gtctgaaggt ttcagacggc attctgtcat gcggcggttc ggggttcgcg gacgggcagg
     1621 acaagcaggg ctgccgtgcc ggcgagtacg gcatcggcgt accacatcca gccgtagtca
     1681 ccgaattgtg taatcacgat gccgccgatg tacgagccga ggaatccgcc gatttggtgg
     1741 gtgagcatca ccagtccgaa cagggtggcg aggtagcgcg tgccgaagag tttgccggta
     1801 acggcggcgg tcggggcgac ggtggcgagc catgtgaatc cgagtgcggc ggcgaaaatg
     1861 taaaagttga ggtcggtttt gggtgagaag atgtaaatca gcaccatggc gacgcgtgag
     1921 gcgtagagtc cgaacaggat gtgttttcct ttgaagcgtc cggtgcatca gcctgagaac
     1981 acgcaaccgg cgatgtttgc cagtccgatg atggcgatgg atgtcgaggc gacggtggcg
     2041 ggcagtccgc acagggcgac ttccgtgggt aggtgggtta cgagaaaggc gatgtggaag
     2101 ccgcaggcga aaaagctcag gtgcagcagg atgtagcttg gggttttgaa ggcggttttg
     2161 actgcttctc cgaggctttg tccgtgtgtg gcttgggtgt gttgggtgtg ggcggcgttg
     2221 ttgccgccgg caagccacca tgagacgggc agaatcagca gggcgattgc gccccaaacg
     2281 taaaatgtac ccgtccagcc gacttcgggc aggacgacga gtccttgagt ccttgaacca
     2341 gcggtgcgaa caggaattgt cctgccgaac cgcctgcgtt gaccagtccg gatgccaagc
     2401 cgcgtttgtg tgtggggact tgggcggcga cctgtcccat aatgatggaa aacccgccgg
     2461 aaccggtgcc gaatgcgagc agcagcccga cggcaatcat cagcccccaa taagtcggga
     2521 tgttggaggc aatcaggcag gcgcagacga gcaggagggc accgccgctt aataccctga
     2581 acgcgccgaa acggtcggca agcgcgccgg acaatggttg caatacgccc cacatcagtt
     2641 ggaaaacggc gatgatgagg ctgaactggg caatgttcaa ttcggtggtg ttgacgacgg
     2701 gttggacgaa cagtccgagc gtcatcctca tgccgatggt aatcagcagg atgaatgcgg
     2761 cggcggcaat gaccgcccag agtttgggtg ttttcgatgc ggtgtgcgtc ataatatgta
     2821 ttttgagtcg gttaagtttg cgaattttaa caaaccggat gattcgggct aaataattcc
     2881 tgattctttg gatatagcca acggtatgtc tgccgatgcc gtctgaaagg gcttgatggg
     2941 cgtttgcggt tcgggttaat atttgtgccg tatttgttaa ttccagttag tatagtggat
     3001 taactttaaa ccggtacggc gttgccccgc cttagctcaa agagaacgat tccctaaggt
     3061 gctgaagcac cgggcgaacc ggttccgtac catttgtacc gtctgcggcc cgccgccttg
     3121 tcctgatttt tgttaatcca ctataaaaat gccgtctgaa acggtttcag acggcatttc
     3181 gatgtcggcg gcggctttgc ggaatcagcc tttgaagcgt ttgaagacca gcgtaccgtt
     3241 ggtgccgccg aagccgaagg agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc
     3301 gttggcgcag tagtccaaat cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt
     3361 cggcgggatt ttgccgtcgt gtatcgccaa aatgctgtac acggcctcca cgccgcccgc
     3421 cgcgccgagc aggtggccgg tcatggattt ggtcgagctg atgacggttt tgcaggcgtg
     3481 ttcgccgaac gcgcgtttga gggctttggt ttcgttggca tcgcccaagg gggtggacgt
     3541 gccgtgcgcg ttgacgtaat ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg
     3601 ggtaacggca agggcggggc cttcttcgtt cggcgcggtg atatggtaag catcggaact
     3661 catgccgaag ccgacgattt cggcgtagat tttcgcgccg cgttttttgg cgtgttctaa
     3721 ttcttccaac accaatatgc ccgcgccttc gccgatgacg aagccgtcgc ggcctttgtc
     3781 ccacggacgg gaagcggtgg cggggtcgtc gttgcgggtg gagagggctt tcatcgcagc
     3841 aaaaccgccc acgcccaagg tgctgattgc gccttccgcg ccgccggcaa ccattatgtc
     3901 cgcgtcgccg tatttgatca gtcgggcgga atcgccgatg gcgtgcgcgc cggtggtaca
     3961 ggcggaaacc atgccgtagc tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga
     4021 aatcaggttg atcagcgaac cggggataaa gaaagggttg attttgcgcg cgccgccttc
     4081 gattacggct ttgccggtgg cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt
     4141 cacgccgatg cggtctttgt cgaggttttc cacatcgtcc aaacccgaat cggcaatcgc
     4201 ctgcaatgcg gcggcgatgc cgtagtggat gaacacgtcc atccggcgcg cttctttcgc
     4261 gctgatgtat tgtccgatgt cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc
     4321 ggatgcgtca aagcgggtaa tcgcgccgat gccgcttttg ccggcgagca ggttgtccca
     4381 agcctctgcg gcagtgttgc cgacagggga aacctgacct aagcctgtaa tgactactct
     4441 tctctgactc atgataacct cgctgttggt tgtcggaatg gggggcatat gcggctgtcg
     4501 tgcagatgcc gtctgtaatt tgcggcaggg gttcaaacag tttgccatat aagggaaaag
     4561 cctctattgc gcggtgcagc agaggctgtt gtgtcgggcg acgaccggtt agccgttgtg
     4621 ggcattgatg tagtcgatag ccagttggac ggtggtgatt ttttcggcat cttcgtcggg
     4681 gatttcgcag ccgaaggctt cttccaaagc cataaccagc tccacggtat ccaaagaatc
     4741 cgcgcccaag tcgtcttgga aggaagattc gtttttcacg tcggcttcgt ttacgcccag
     4801 ttgttcagca ataattttct taacttgttg ttcgatgttt gacatatcag tcgttccttt
     4861 atgccttgcg gcaggttgtt taagggaaat aaatcggtgg tattgtaccg atttttaata
     4921 gagttttcta tctaatgcct attatatcaa tatttgcgga tttgtacatt tttgggtgcg
     4981 gcgggttttg tcgttcaagt ttgacctgtg tgccgtatgt ttggcgggat ttcggttaaa
     5041 atggcggcat ttccatctga agcagaaagc cctgtcatgt atccacttgc ccgccgcatc
     5101 ctgtttgcac tcgatgccga aaaagcccac cacttcacgc tcgacgcgct caacacggtt
     5161 tataaattgg gcctgattcc cgtaaccgac aaccgtacca aacctataaa actgatgggt
     5221 atggatttgc ccaaccctgt cgggcttgcc gcaggtctcg acaaaaacgg tgaatacatc
     5281 gacgctttgg gcgcgctcgg tttcggcttc ctcgaaatcg gcacggttac gcgcaacccg
     5341 cagcccggca acccgcagcc gcgcctcttc cgcgttcccg aacaccaagg catcatcaac
     5401 cgaatgggtt ttaacaacca cggcatcgac gcgatgatac gcaatattga aaaaagcaaa
     5461 tatcaaggcg tattgggcat caacatcggc aaaaacgcgg ttacgcccat ccaaaacgcc
     5521 gccgatgatt atttaatctg cctcgaaaaa gcctacgcgc acgcaagcta cattaccgtc
     5581 aacatttcct cgcccaacac caaaaacctc cgcgcgctgc aaggcggcgg cgaattgggc
     5641 gcattgctcg aagccctgaa aaacaaacag gcgcagcttg ccgccgccca cggcaaatac
     5701 atcccgctag ccgtcaaaat cgcccccgat ttggatgaag cacaaatcga agacatcgcc
     5761 cgcgtcgtca aatccgtcga aatggacggc atcatcgcta ccaacaccac catcgacaaa
     5821 tcaagcctcg gcagccatcc gctcgcaggc gagcagggcg gtttgagcgg ttttcccgtg
     5881 cgcgaaaaaa gcaaccgggt gctgaaaaaa ctggcggaac acatagacgg cgcattgccg
     5941 attatcggcg taggcggcat tatggaaggc ggggacgcgg cagagaaaat ccgcttgggc
     6001 acgaccgccg tccaagtgta cagcggattg atatacaaag gcccggcgtt ggtcaaagaa
     6061 tgtttgaagg cattggcgcg atgacgcgcc ccacccaaaa tgccgtctga acgcacgttt
     6121 tgccgttcag acggcatttt catagattgg tatccgactg ttgaagaggc aatcagccgt
     6181 catttggaag acttttcgta acaggcgtca atcgaaaggg gcggaatgac ggggcaggca
     6241 aaccgatgcg tgaacacggg gcaggcaacc ggagtaaaaa atgaaccttg atttaaccgc
     6301 tcaaaaagtc cgcctttctt ggaaggatat tctgtggggg tatgagaata aatacttggg
     6361 ttgggctgat gtggcagctt atgcccgaaa aatgacgctt tcagatcatg atgaacgcgt
     6421 gttcaagcta tctttaacca acaaatccaa tatttttgaa ttaaagcctg ttctagaaga
     6481 tttggcttcg gaaacgaggg gttattcccc taaaaattgg ctgtacatcc tcctaaatga
     6541 cgtattccat agaaaggaag aatttgagga tcctctgggg gaagttgaaa aaatttatgc
     6601 ggattttgat tacccggaag aaatagaatc atttgtcagg tatatgccgc ccaaagacgg
     6661 ctatatccct tctaatcata gttatgaaga aaatattgcc cggttatatt ctcactggga
     6721 acactatttg aacaacggcg gcgggcaggg ttaaaaccgg caatccgatg ccgtctgaag
     6781 cgttatccgg cgttcagacg gcattttgtt ttccgacagt ttataaactg tcgttgtttc
     6841 ttgacagaaa caacgacctt atttatttga aacgattgga ggacatgatt atgggttttt
     6901 ggggtagtgt gggaaaagca gcaaaagcag taggcgaggg attgattgaa gccggcaatc
     6961 agcataaggc gttgaaaatg gaatatgcgg agaaatcaag tgaggagctg catgaaatcg
     7021 taaagagtga tggttttttt aaaaattcca cacgggagaa aggtgcggct tatgctgttt
     7081 taaaagagcg tggcgaggtt tgaacgggaa acggcggcat ttgccgctgt ttttttatcg
     7141 gcaggcatcc gtccgaatat cggggcaagg tttcagacgg catcgaaggt tgctatgata
     7201 tagtggcttg actttaaacc ggtacggctc cgccttgccc tgatttgaag ttcatccgct
     7261 atcacattcc cgccatcctt tcaaacggaa tccgaaatgt ccgaccaccg cctcgacacc
     7321 gcccgccgcc attccctgtt cctcgcccgc cagctcgaca acggcaaact caagcccgaa
     7381 attttcctgc ccatgctgga caaggcgttg acggatgagg atttccaagc ctttgccgac
     7441 tgggacaaaa tccgcgcgga agaaagcgag gaagaattgg cgcggcagtt gcgcgagttg
     7501 cgccgttatg tggtgtcgca gattatcgtg cgcgatataa accgcatcag cgatttgaac
     7561 gaagttaccc gcacgattac gctgtttgcc gattttgccg tcaataccgc gctggatttc
     7621 gcctacgcct attatcagga catgtacggc acgccgatcg ggcgttatac caaatcgccg
     7681 cagcatttga gcgtggtggc gatgggcaag gcgggcggct atgagctgaa cgtgtcttcc
     7741 gacatcgatt tgattttcgt ctatcccgaa tcgggcgaca ccgacggcag gcgcgaacgg
     7801 gggaatcagg agttttttac caaagtcggg cagaaactga ttgcattgtt gaacggcatt
     7861 actgccgacg ggcaggtgtt ccgcgtcgat atgcggctgc ggccggacgg tgattcgggc
     7921 gcgctggtgt tgagtgaaac cgcgctggag caatatctga ttacgcaggg gcgcgaatgg
     7981 gaacgctatg cgtggtgcaa aggccgcgta gtcacgccgt atccgaacga catcaaatcg
     8041 ctggtgcgcc ccttcgtgtt ccgcaaatat ctggattacg gcgcgtatga ggcgatgcgc
     8101 aacctgcacc gccaaatccg cagcgaagtc agcaaaaaag gcatggcgga caacatcaaa
     8161 ctcggcgcgg gcggcatccg cgaggttgaa tttatcgccc agattttcca gatgatacgc
     8221 ggcggccaaa tgcgcgcgct gcaactgaaa ggcacgcagg aaacgctgaa gaaaattgcc
     8281 gagatgggca ttatgccgtc tgaaaacgtc gaaaccctgc ttgccgccta ccgcttcctg
     8341 cgcgatgtcg aacaccgcct gcaatactgg gacgaccggc aaacccaaac cctgccgatc
     8401 tcgcccgaac agcggcaact gctcgccgaa agcatgggtt tcgacagtta tgccgctttt
     8461 tcagacggcc tcaatgttca tcggaacaaa gtcaatcagt tgttcaacga aattttgagc
     8521 gaacctgaag aacaagcgca aagcaatagc gaatggcaat gggcatggca ggaaaaaccc
     8581 gacgaagaag aacggctagg ccgtctgaaa gaacacgggt tcgatgccga agccgtcacc
     8641 gcaaggctcg aacaaatctg ccacggacat aaataccgcc gcctttccgc acacgcccag
     8701 ccgcgctttg acaccattgt gccgctgttc gtacaggcgg cggcagagca aaacaacccg
     8761 acagatacgc tgatgcggct gttggatttt ctcgaaaaca tcagcctccg ctccgcctat
     8821 ctcgccttcc tcaacgaaca tccgcaaacc ttggcacaac tggcgcagat tatgagccaa
     8881 agctcgtggg tggcggcgta tctgaacaaa tatccgattt tgttggacga actcatcagc
     8941 gcgcagcttt tggataccgc gtttgattgg caggcactcg ccgccgccct ttcagacggc
     9001 atcgaagcct gcggcggcga taccgaagcg caaatggaca cgctgcgcca tttccaacac
     9061 gcccaagtct tccgcctcgc cgtccaagac ctcgccggat tgtggacggt agaatccctc
     9121 tctgaccaac tctccgccct cgccgacacc gtcattgccg ccgccctttc gtgcgcgtgg
     9181 gcggatatgc ccaaaaaaca ccgcgacacc ccgcaattcg ccgtcatcgg ctacggcaag
     9241 ctcggcggca aagaactcgg ctacgcctcc gacctcgact tagtctatct ttacgacgat
     9301 ccccatcccg aagcaggcga cgtgtacagc cgcctcgccc gccgcctgac caaccggctt
     9361 tccgccgcca ccggcgcggg cagcctctac gaaaccgacc tgcgcctgcg ccctaatggc
     9421 gacgccggtt tcctcgcgca cagcatcgcc gcctttggaa aataccagcg tgaaaacgca
     9481 tggacatggg aacaccagtc cctcacccgc gcccgcttca tctgcggcac acccgaaatt
     9541 cagacggcat tcgaccgcat ccgcaccgaa atgctgactg ccgaacgcga ccaaaccgcc
     9601 ttggcaggcg aaatcatcga aatgcgcgaa aaaatgttcc ccacccatcc gcctgccgac
     9661 agcaacgtca aatacgcgcg cggcggcgtg gtcgacgtcg aatttatcgt ccaatatctg
     9721 atacttgccc atgcccgcca gtatccgcaa ctcttggaca actacggcaa catcgcccta
     9781 ttgaacatcg ccgccgactg cggtttgatt gacaaaaccc tcgccggaca aagccgcacc
     9841 gcctaccgcc tctaccgccg gcagcagcac aacaccaaat tgcgcgacgc ggcaaaaacc
     9901 gaagtaagcg acgaattgct gtcccattac ggcaatgtca ggaaattgtg gcgggaagtg
     9961 ttcggggagg aagcggcaac cgcctgaaca aaaaatgccg tctgaagcct gacaatccgg
    10021 gtttcagacg gcatttgttt ttggctgtgt ctgaacaatc ggtacgattt cggttgtttg
    10081 caaattgaaa ggcggttatt cctatgttca ggcggcaaac cgaaatattt tccccaaccg
    10141 atagggcgat atcaaagcct attcgaatac cgcctttacg gggaaggggc tttccgagct
    10201 tgaccatggt caggctcggc aaatcagccc gcatcctgtc gatgcgtccg tccaaatcgg
    10261 tttggaacgc cgcaatgtga tatccgccta tcaaggtttt gcgtgcgtgt gtcggcgagg
    10321 gctttgccaa aaccgaaatc aaaatcaagt tgtcaggcat ccttccgttt ctattaaaat
    10381 agcgcattcc atttttcaga cggcatcctt atgtttcccg accaatccgc ccccaatctg
    10441 ctgcaaggct tgaaccccga acaactctcc gccgtaacct ggccgccgca atccgccctc
    10501 gttttggcag gcgcaggcag cggcaaaacg cgcgtgctga ccacgcgtat cgcatggctt
    10561 ttgcaaagcg gacaagccag cgtacacagc attatggcgg taacgtttac caacaaggcc
    10621 gccaaagaga tgcaaacccg attcggcgca atgattccca tcaacgtccg cgctatgtgg
    10681 ctcggcacgt tccacggttt gtgccaccgc tttttgcgcc tgcaccaccg cgacgcaggc
    10741 ctgccgtctt cctttcaaat cctcgacagc ggcgaccagc tttccctcat caaacgcctg
    10801 ctcaaaagcc tcaacatcgc cgaagaaatc atcgcgccgc gttcgctgca aggctttatc
    10861 aacgcgcaaa aagaatccgg tttgcgcgct tccgtcttgg gcgcgcccga tccgcacaca
    10921 agccgcatga ttgggtgcta cgccgaatac gacaaaatct gccaacgcga aggcgtggtc
    10981 gatttcgccg aactcatgct ccgcagctac gaaatgctgc aaagtaacga aatcctgcgc
    11041 cggcactacc aaaaccgctt caaccacatt ctggtcgacg agtttcaaga caccaacaaa
    11101 ctgcaatacg cctggctcaa actcatggcg ggcggtaacg cagcggtatt tgccgtcggc
    11161 gacgacgacc aaagcattta ccgcttccgc ggcgcaaacg tcggcaacat gaccgcactg
    11221 atggaagaat tccacatcga cgcgcccgtc aaactcgaac aaaactaccg ctccgtcggc
    11281 aacatcctcg ccgccgccaa cgccgttatc gaaaacaacg acgaacgtct cggcaaaaac
    11341 ctgcgcaccg acgccgaagc aggcgacaaa atccgctact actccgcctt taccgacctc
    11401 gaagaagccc ggttcatcgt ggatgaaacc aaagccctcg aacgcgaagg ctgggatttg
    11461 gacgaaatcg ccgtcctcta ccgcagcaac gcccaatcgc gcgtcatcga acaaagcctg
    11521 ttccgcagcg gcatccccta caaaatctac ggcggcctgc gcttttacga acgccaagaa
    11581 atcaaacacg ccctcgccta cctgcgcctc gccgtcaatc ccgacgacga caacgccctc
    11641 ttgcgtgtca tcaacttccc accgcgcggc atcggcgcgc gcaccgtcga aaaccttcag
    11701 acggcctcaa acgaacaagg catcaccctc tggcaggcag cctgcaacgc cggcgcgaaa
    11761 gccgccaaag tcgccgcctt cgtccgcctg attgaagccc tgcgcaacca agtcggacaa
    11821 atgcacctgt ccgaaatcat cgtcggcatc ctcaaagaca gcggtctgac cgagcactac
    11881 cgaacccaaa aaggcgacaa ccaagaccgc ctcgacaacc ttgacgaact cgtcaacgcc
    11941 gccatcgagt tcaaacccga agacagcaac tttgaaaccc tgcccgaaaa catttcagac
    12001 gaccccgcct tccccatcct cgccttcttg agcaacgccg ccctcgaatc cggcgaaaac
    12061 caagcagggg caggcgaaaa agccgtccaa ctgatgaccg tccacgccgc caaaggcttg
    12121 gaattcaacg ccgtcttcct caccggtatg gaagaggggc gtttccccag cgaaatgagc
    12181 cttgccgagc gcggcggcct cgaagaagaa cgccgcctca tgtacgtcgc catcacccgc
    12241 gcccgcaaac gcctctacat caccatggca cagcaacgca tgctgcacgg acaaacccaa
    12301 ttcggcatcg catcccgctt cgtcgaagaa atcccgccag aagtattgca ctacctgtcc
    12361 gtcaaaaaac ccgccttcga cagctacggc aacacgcgcc aaaccaccgt acaacgggac
    12421 aaaatcatcg atgacttcaa acagccgcaa acctacgcag gcttccgcat cggacaaaac
    12481 gtccgtcacg ccaaattcgg caccggcgtg attatcgatg ccgcagacaa aggcgaatcc
    12541 gcccgactga ccatcaactt cggcaaacag ggcgtgaaag agctggatac caagtttgcg
    12601 aaattggaag cgatgtaaat ttgaaatgca ggccggatat tcgtatccga caaaaaaaca
    12661 tttgacgcgt ttatcgtttc cgaaaaaccg ctgttggaaa tgtcggattc gagaatccga
    12721 cctacggcaa aaaaccgtag caaggacaag gcaaatagtc gtctgaaaac gggaagggca
    12781 attttgccgc agccgccgcc gtcattcccg cgaacctaca ttccgtcatt cccacgaaag
    12841 tgggaatccg gaacgcaaaa tctaaagaaa ccgttttatc cgataagttt ccgcaccgac
    12901 aggtctggat tcccgcctgc gcgggaatga cggcggcgga ttcggcggag ttggtggatt
    12961 tggcgggctg aagcccgccc ttgtatatcg gaatt
//
LOCUS       DAWTCI010000060        12760 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWTCI010000060 DAWTCI010000000
VERSION     DAWTCI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.060"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..365
                     /locus_tag="WM481_001584"
     CDS             <1..365
                     /locus_tag="WM481_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9080870.1"
                     /translation="GIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMG
                     APVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSG
                     KYAFARRIKKNDPSRFLN"
     gene            384..602
                     /locus_tag="WM481_001585"
     CDS             384..602
                     /locus_tag="WM481_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080871.1"
                     /translation="MSMPEMPKWYGDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRAYLLALIEGLENPYRKV"
     gene            747..1091
                     /locus_tag="WM481_001586"
     CDS             747..1091
                     /locus_tag="WM481_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFA9080872.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            1091..1783
                     /locus_tag="WM481_001587"
     CDS             1091..1783
                     /locus_tag="WM481_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFA9080873.1"
                     /translation="MSEILRQPGILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFRLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLPLASKSVTNPIAIEITSSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            1850..3070
                     /gene="argJ"
                     /locus_tag="WM481_001588"
     CDS             1850..3070
                     /gene="argJ"
                     /locus_tag="WM481_001588"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFA9080874.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDQHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTCDEARQAAYAAARSPLVKTAFFASDPNLGRLLAAIGYADVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            3130..4527
                     /locus_tag="WM481_001589"
     CDS             3130..4527
                     /locus_tag="WM481_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFA9080875.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWIALPPGLPVIVWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLENILMWIVAAGLVCGAAGGIFARMLHRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPVGLAAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSPRPFYHASGMRFRRRVLQETAAQTGNAPA
                     RPQAANSKTGMPSEN"
     gene            4715..6106
                     /gene="hrpA"
                     /locus_tag="WM481_001590"
     CDS             4715..6106
                     /gene="hrpA"
                     /locus_tag="WM481_001590"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFA9080876.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKIEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLEEIKKTVAENQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKCRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEVV"
     gene            6174..7676
                     /locus_tag="WM481_001591"
     CDS             6174..7676
                     /locus_tag="WM481_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFA9080877.1"
                     /translation="MNIKEFMSNYTNHPVLFIGTGMSLRYLDNSYTWDGLLSKIAIDL
                     FGDDREYLNIKSRYCEDGRFQYEEIAEELQSKFDKVLENDPDGRFKEINDKFFENMRA
                     GNTLSRFKIYISTLLSQLNYKDNSNTELSELKKARKNVGSIITTNYDKLAQDIFEFNP
                     LIGNDILLSNPYGSVYKIHGCVDDPSKIIITKKDYEKFKEKYELIRAQLLSLFIHNPI
                     IFLGYNVGDENIKEILKTIFTYVEPNSPSANKIRRNFLLVEYEPESNNEDIVEHDIDI
                     TGFSTIRINKIKTDNFSQIYKALAELTLPISAMDVRKFQSIAKEIYTGGNIKVSFTED
                     MDNLNNSDKVVAIGSTKTISYNFQTTSEMMSNYFKIIEEENSQLLKLIDKHSIASTQY
                     FPIYGFSRICSDIHKEAVLKRQQKEKLDHFIEEINRRCKNNHSSIQSILDDENISDTY
                     KNDAIAWGIWNNQLSEDEVENYLKNFVNKKNTHYKRLLCMFDYKKYADTV"
     gene            7903..9480
                     /gene="lpt3"
                     /locus_tag="WM481_001592"
     CDS             7903..9480
                     /gene="lpt3"
                     /locus_tag="WM481_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HFA9080878.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSKIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIADK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRNGKAEYVYPQ"
     gene            9546..>12760
                     /locus_tag="WM481_001593"
     CDS             9546..>12760
                     /locus_tag="WM481_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFA9080879.1"
                     /translation="MQNMKNQVRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLTYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRPPEVKQLTSSENAGDQDLSAKLKQKQLDKKQHRTQIRATKEAG
                     YEQIHRALLTGLIANVGMKSPDSNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TRLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLAVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQEYDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYHERLPDFYMADSVSEGLCPANPQQTTPSPVGEGWGEGKTVAAQTNFSATSA
                     SPLPNPLPQEREQSASVSTVSGSLKTMSCEARLNFCEAKTKTESSLHSQRLSENYTPP
                     FSDGLRPANPQQTTPSPVGEGWGEGKTVAAQTNFSATAANPLPQEREQSASALTVSDD
                     PKPKKQPASQKGRLKPLPLADIRTFEAWLKTAERDNPRLLFLSRDDLMQHAAAHITEE
                     QFPKHWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPGMIREKIQ
                     LQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTAGDIRILEQINQ
                     DEWAAFRLPEHCYFNLRIIDDGGQELAMGRDLIQIQQQLGKAATTTFRDNTQEFERDN
                     VTAWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTSAAAGHAHRLGVIEL
                     MKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDKLPRNEK
                     AFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPG
                     FATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKTDGLAKQ
                     GQPVSDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKM"
BASE COUNT         3296 a         3728 c         3042 g         2694 t
ORIGIN      
        1 tgggtattgc ctaccgctac ggcggcacat cggtttctac cggttttgac tgcagcggat
       61 tcatgcagca catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg tcggcggaac
      121 aggcgcggat gggcgcaccc gttgcccgaa gcgaattgca gcccggggat atggtgtttt
      181 tccgcacgct cggcggcagc cgcatttccc atgtcggact ttatatcggc aacaaccgct
      241 tcatccacgc gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc cacaaatatt
      301 ggagcggcaa atatgcgttc gcccgccgga tcaagaaaaa cgacccgtca cgctttctga
      361 actgatttcc caaggaatac gcaatgagta tgcccgaaat gcccaaatgg tacggcgatg
      421 acggacagat cgtatcctgt accgaaaagg tcaaagtgat gtccgaaaat atggccgagc
      481 tgtatcagac ggcacaagac gcgtttgaag acgcgctgct gatgggttgc ggcgaacggc
      541 agttgcgcgc ttacctgctc gcgctgattg aaggtttgga aaatccctac cgcaaagtct
      601 gaacacgccc cggttgctgc ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc
      661 ttcggctttt cagacggcat atttgacgtt atgattaaac agttaacaag atttatcaca
      721 acgccgtcaa aagacagaca cacaacatga acatcatccg cgcgctcctc atcatcctcg
      781 gctgcctcgc cgccggcgaa accgccgttt tcctagcagg catcaaactg cccggcagca
      841 tcgtcggcat gggcgtgctg tttgcgcttt tgcaggcggg ttgggtcaaa acgtcttggc
      901 tgcaacagct taccgacgcg ctgatggcaa acctgacgct gttcctcgtg ccgccctgcg
      961 tggcggtcat cagctatttg gatttgattg ccgacgattg gttttcgata ctggtttccg
     1021 cctccgccag cactttgtgc gtactgctgg ttacgggcaa ggttcaccgc tggatacgga
     1081 gcattatctg atgagcgaaa tcctcaggca gcccggcatc ctgcttttcc tcacgcttgc
     1141 cgtgtacgcg cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt
     1201 actcgtcagc actatcgtgc tgattgccta cctgaaaatc ctcggtatcg attatgcggt
     1261 gtaccacaac gccgcgcaat tcattgattt tcggctgaaa cccgccgtcg tcgtgcttgc
     1321 cgtgccgctc taccaaaacc gccgtaaaat cttcaaccag tggctgcccg tcatcgtttc
     1381 gcagcttgcg ggcagcgtta cgggcattgt tacggggatg tattttgcca agtggctggg
     1441 cgcggaacgc gaagtcgtcc tcccgctcgc gtccaaatct gttaccaacc ccatcgccat
     1501 cgaaatcacc agctccatcg gcggcattcc cgccattacc gccgccaccg tcatcattgc
     1561 cggtctggtc ggacagattg ccggttacaa aatgttgaag aacacggtcg tcatgccctc
     1621 gtccgtgggt atgtcgctcg gcacggcttc gcacgcgatg ggcattgccg cctcgctcga
     1681 acgcagccgc cgcatggcgg catacgcggg gctggggctg acgttcaacg gcgtgctgac
     1741 cgcgctgatt gcgccgctgc ttatccccgt tttggggttc tgaacccgtt tcagacggca
     1801 tttcaaccca tgccgtctga acgccgacac actcgcaagg agaaccgtta tggctgtcaa
     1861 cctgaccgaa aaaaccgccg aacaactgcc cgacatcgac ggcattgccc tctacaccgc
     1921 ccaagcaggc gtgaagaagc ccgggcatac cgacctgaca ctgattgccg tagccgccgg
     1981 cagcaccgtc ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc
     2041 caaatcgcac cttttcgacg aagacggcgt gcgcgccctc gtcatcaaca cgggcaacgc
     2101 caacgcgggt acgggcgcac agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc
     2161 ccggcaaatc ggctgcaaac cgaaccaggt gctgcccttc tccaccggcg tgattctcga
     2221 accgctgccc gcagacaaaa tcatcgccgc cctgcccaaa atgcagcctg ccttctggaa
     2281 cgaagcggca cgcgccatca tgaccaccga caccgttccc aaagccgcct cgcgcgaagg
     2341 caaggtcggc gaccaacaca ccgtccgcgc cacaggcatt gccaaaggct cgggcatgat
     2401 tcatcccaat atggcgacca tgctcggttt catcgccacc gatgccaaag tttcccaacc
     2461 cgtcctccaa ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga
     2521 cggcgacacc agcaccaacg acagcttcgt catcatcgcc accggcaaaa acagccaaag
     2581 cgaaatcgac aacatcgccg acccgcgtta cgcccaactc aaagaattgt tgtgcagcct
     2641 tgcgctcgaa ctcgcccaag ccatcgtccg cgacggcgag ggcgcgacca agttcatcac
     2701 cgtccgcgtc gaaaacgcca aaacctgcga cgaagcccgc caagccgcct acgccgcggc
     2761 acgttcgcca ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg gcaggctgct
     2821 cgccgccatc ggttatgccg acgttgccga cctcgatacc gacctcgtgg aaatgtatct
     2881 cgacgatatt ttggttgccg aacacggcgg acgcgccgca agctacaccg aagcacaagg
     2941 gcaggcggtg atgtcgaagg acgaaatcac cgtccgcatc aagctgcatc gcggacaagc
     3001 cgccgccacc gtctatacct gcgacctgtc gcacggatac gtttccatca acgccgacta
     3061 ccgttcctga cccgacacgg cttcagacgg catacataaa atgccgtctg aaccgtcgga
     3121 caacatacca tgacctccac attcccccgc cgcctcgccc gcaaaatccg ccaaacccgc
     3181 cgcctgtcgc gcaaaagcat cgcctttctg ttccttctgg caggttcggc actcgtcgcc
     3241 ctgaccgcac tgttttttgc ccatcttgcc gattttgcgc tggaactgaa cgccaaactg
     3301 gttcaacaat acccgtggtt cgcatggatt gccctgccgc cgggactgcc cgtcatcgtc
     3361 tggctcacac gcaaattcgc ccccttcacc gccggcagcg gcatccctca agtcatcgcc
     3421 tcactgtcgc tgccctacgg cgcacaaaaa acgcggctga tacgcctcgg gcaaacgctg
     3481 ctgaagattc cgctgacctt tttgggtatg ctgttcggcg cgtccatcgg acgcgaaggc
     3541 ccgtccgtgc aggtcggcgc ggcagtaatg ggcgcgtggg gcgcgtggtg caaaaaacac
     3601 ggtttggcgt ttaagggtat gcaggaaaac gatttgatgg cggcgggcgc ggcgggcggt
     3661 ttggcagcag cgttcaacgc gccgctggcg ggcgtgattt tcgccattga ggaactgggg
     3721 cgcggcatca tgctgcgctg ggagcggcag attctgctcg gcgtactcgc cgccggtttc
     3781 atacaggtcg ccattcaggg caacaacccg tatttttcgg gtttcaacgg cggcgttttg
     3841 gaaaatattt tgatgtggat cgtggcggcg gggctggttt gcggcgcggc tggcggcata
     3901 tttgcccgta tgctccatcg cggtgcggcg gcgtttgcac cgcgcaagat acgcggcttc
     3961 atccgcaacc gtccgctgct gctggcggca ctgatggggc tgctgctcgc cctgctcggc
     4021 acgttctacc aaggcaaaac ctacggcacc ggctaccacg aagccgccca agccctgcac
     4081 ggcatctacg aagcccccgt cggactcgcc gccgccaaat ggctcgccac cgtattcagc
     4141 tattgggcag gcatcccggg cggcattttc actccctcgc tgaccatagg tgcggttttg
     4201 ggcgagcata tcgccgccat cgccgacata tcgcagggtg caaacatcat cgtcctcatc
     4261 tgcatggcgg catttttagc gggcgcgaca caatcgccga ttacttccgc cgtcgtcgtc
     4321 atggaaatga ccggcgggca aagcctgctg ttttggatgc tgattgcctg cattttcgcc
     4381 tcgcaggttt cgccgcgtcc gttctaccac gcatcgggaa tgcgcttccg ccggcgcgtg
     4441 cttcaggaaa ccgccgccca aaccggcaat gcgcccgcaa gaccgcaagc cgcaaacagc
     4501 aaaacgggaa tgccgtctga aaattaaaac gcccccgatc aaacgccggc agccgccttg
     4561 attcaaatac cgttccgctg cgcttgaaat ttcagcaaca atgccgtctg aacgacagaa
     4621 tgcggttttc agacggcatt tccccatccc gatattgttc aaacaaaacc gaagcgtttg
     4681 ctataattgc actttttacc gcacacgcac gcccatgttt cccgatttct cccaaaccct
     4741 ctccaaagac cgccacttcc tgcaatccgc cttcaaaaat cccaacaaat acggcggctt
     4801 gtccaaaatc gaagaaaaat accgaaaatc gcacgaaatc ttcttgaaac gtttggccgc
     4861 attgccaaaa cccgaattcg acaacaccct gcccgttcac gaaaaactcg aagaaatcaa
     4921 aaaaaccgtt gccgaaaatc aggtaacgat tatctgcggc gaaaccggtt cgggcaaaac
     4981 cacgcagttg cccaagattt gtttggaact cgggcgtggg gcggcaggat tgatcgggca
     5041 tacccagccg cgccgtttgg ccgcgcgttc ggtggcggag cggattgccg aagagctgaa
     5101 atccgaaatc ggcagcgcgg tcggctataa agtgcgcttt accgaccaca cctcgcgcga
     5161 tgcctgcgtc aagctgatga ccgacggcat cctgctggcg gaaacccaga ccgaccgtta
     5221 tctcgccgcc tacgacacga ttatcattga cgaagcgcat gaacgcagcc tgaacatcga
     5281 cttcctcttg ggctacctaa aacaactcct gccgcgccgc cccgatttga aagtcatcat
     5341 cacatcagcc accatagacg cagaacgctt ctcccgacac ttcaacggcg cgcccgtact
     5401 ggaagtgagc gggcgcacct atcccgtcga aatcctctac cgaccgctga ccggcaaaga
     5461 cgaagacgac gcagaagtcg agctgaccga cgcgattgtc gatgccgccg acgaattggc
     5521 acgctacggc gaaggcgata ttttagtatt cctgccgggc gaacgcgaaa tccgcgaagc
     5581 ggcagaagcc ctgcgcaaat ccacactgcg ccgcaatgac gaaatcctgc ccctgtttgc
     5641 acgcctgtcg cacgccgaac agcacaaaat cttccaccct tcaggcgcaa aatgccgcat
     5701 cgtgctggcg accaacgtcg ccgaaacctc gctcaccgtg ccgggcatca aatacgtcat
     5761 cgacaccggc ctcgcgcgcg tcaaacgcta ttccgcgcgg gcaaaagtgg aacaacttca
     5821 cgtagaaaaa atctcccaag ccgccgcccg ccaacgctcc ggccgctgcg gacgcgtctc
     5881 agcaggcgtg tgtatccgac tgttttcaga agaagatttt aacagccgtc ccgaatttac
     5941 cgatcccgaa atcgtccgca gcaacctcgc cgccgtcatc ctgcgcatgg catcgctgaa
     6001 cttgggcgac gtagcagcat tcccgttttt agaaatgccc gactcgcggt atatcaatga
     6061 cgggtttcag gtgttgttgg aattgggggc agtggaggtt gtctgaaaac aggcagatcg
     6121 ggcgtttatg cccaacgaga accttattgt cactattatt agggattaat acaatgaata
     6181 taaaagaatt tatgtctaac tataccaacc atcccgttct ctttattgga acaggtatga
     6241 gtttgagata cttagataat tcatatactt gggatggttt attatctaaa attgcaatag
     6301 atttatttgg agatgatagg gaatatttga acatcaaatc acggtactgt gaagatggta
     6361 gattccaata tgaagagatt gcagaggaat tacaaagtaa atttgataaa gttttagaaa
     6421 atgaccctga tggtagattt aaagaaataa atgataagtt ctttgaaaat atgagggcgg
     6481 gaaacacctt gagcaggttt aagatttata tatccaccct gctctcacaa ttgaattata
     6541 aagataattc taatacagaa ttatctgaat taaaaaaagc gagaaaaaat gtagggtcaa
     6601 tcattacaac aaattatgat aaattagccc aagatatttt tgaatttaac ccactaattg
     6661 gtaatgatat tcttttaagc aacccttatg gctcagtata caaaatacat ggttgtgtgg
     6721 acgatccatc aaaaattatt attaccaaaa aggattatga gaaatttaaa gaaaaatatg
     6781 aacttattag agcccaacta ttatcgttat ttattcacaa tccaatcata tttcttggat
     6841 ataatgttgg tgacgaaaat atcaaagaaa ttttaaaaac aatctttact tatgtagaac
     6901 caaactctcc ttcagctaat aaaattcgta ggaatttttt actcgtagaa tatgaacctg
     6961 agtctaacaa tgaagatatt gttgaacatg atatagatat aactggattc tctactatcc
     7021 gtattaataa aatcaaaaca gataacttct cacaaattta taaagctctt gcagaactaa
     7081 cactaccaat ctcagctatg gatgtacgta aatttcaatc tatagcaaag gagatttata
     7141 ctggcggtaa cattaaagtt agctttacgg aagatatgga caatttaaat aatagcgata
     7201 aagtggttgc tattggttca actaaaacta tcagctacaa ctttcaaaca acatcagaga
     7261 tgatgtcaaa ttatttcaaa ataattgaag aggaaaattc acaacttcta aaattaatag
     7321 ataaacatag tatagcatct acgcaatatt ttcctattta tggatttagt aggatatgtt
     7381 ctgatataca taaagaagct gtactaaaac gccaacaaaa agaaaaatta gatcatttta
     7441 ttgaagaaat aaataggcgt tgtaaaaata atcattcatc catccaatca attttagatg
     7501 atgaaaatat ttcagacaca tataaaaacg atgcgattgc ttggggaata tggaataacc
     7561 aactttcaga agatgaggtt gaaaattatt taaaaaattt tgtgaataaa aaaaatacgc
     7621 actataaaag actactatgt atgttcgatt ataaaaaata tgcggatact gtctaaactc
     7681 taaaaaataa ggtcgggcat ttatgtccga cctacaaaac tatgcaaaac actaaaaatc
     7741 aagcacgcag ctcaggcatt catgcccaac acaatccgac aaatgacaaa ccactttcag
     7801 acaacctcgt tttttgaaaa acaatctgta aacagatagc tgctgaagaa taccgttgcc
     7861 gagccccaaa acccgtcctg caacttttat tgtgaactgc ccattatgaa aaaatccctt
     7921 ttcgttctct ttctgtattc atccctactt accgccagcg aaatcgccta tcgctttgta
     7981 ttcggaattg aaaccctacc ggctgcaaaa atggcggaaa cgtttgcact gacatttgtg
     8041 attgctgcgc tgtatctgtt tgcgcgttat aaggcaacgc gtttgttgat tgcggtgttt
     8101 ttcgcgttca gcattattgc caacaatgtg cattacgcgg tttatcaaag ctggatgacg
     8161 ggtattaact attggctgat gctgaaagag gttaccgaag tcggcagcgc gggcgcgtcg
     8221 atgttggata agttgtggct gcctgctttg tggggcgtgg cggaagtcgt gctgttttgc
     8281 agccttgcca agttccgccg taagacgcat ttttctgccg atatcctgtt tgccttccta
     8341 atgctgatga ttttcgtgcg ttcgttcgac acgaaacaag agcacggtat ttcgcccaaa
     8401 ccgacataca gccgcatcaa agccaattat ttcagcttcg gttattttgt cggacgcgtg
     8461 ttgccgtatc agttgtttga tttaagcaag atccctgtgt tcaaacagcc tgctccaagc
     8521 aaaatcgggc aaggcagtat tcaaaatatc gtcctgatta tgggcgaaag cgaaagcgcg
     8581 gcgcatttga aattgtttgg ttacgggcgc gaaacttcgc cgtttttaac ccggctgtcg
     8641 caagccgatt ttaagccgat tgtgaaacaa agttattccg caggctttat gacggcagta
     8701 tccctgccca gtttctttaa cgtcataccg cacgccaacg gcttggaaca aatcagcggc
     8761 ggcgatacca atatgttccg cctcgccaaa gagcagggct atgaaacgta tttttacagt
     8821 gcccaggctg aaaaccaaat ggcaattttg aacttaatcg gtaagaaatg gatagaccat
     8881 ctgattcagc cgacgcaact tggctacggc aacggcgaca atatgcccga tgagaagctg
     8941 ctgccgttgt tcgacaaaat caatttgcag cagggcaggc attttatcgt gttgcaccaa
     9001 cgcggttcgc acgccccata cggcgcattg ttgcagcctc aagataaagt attcggcgaa
     9061 gccgatattg cggataagta cgacaacacc atccacaaaa ccgaccaaat gattcaaacc
     9121 gtattcgagc agctgcaaaa gcagcctgac ggcaactggc tgtttgccta tacctccgat
     9181 catggccagt atgtgcgcca agatatctac aatcaaggca cggtgcagcc cgacagctat
     9241 attgtgcctc tggttttgta cagcccggat aaggccgtgc aacaggctgc caaccaggct
     9301 tttgcgcctt gcgagattgc cttccatcag cagctttcaa cgttcctgat tcacacgttg
     9361 ggctacgata tgccggtttc aggttgtcgc gaaggctcgg taacaggcaa cctgattacg
     9421 ggcgatgcag gcagcttgaa cattcgcaac ggcaaggcgg aatatgttta tccgcaataa
     9481 gcagcgtgaa aaccaataaa gacaaattta gatgatgtcg gggaagatgc ccgaccgaca
     9541 agactatgca aaatatgaaa aaccaagtac gcggatcagg catggatgcc cgatccaacc
     9601 cggccaatgt ttcagacggc ctgcaaaaca gttcgggcca tatcggtacc aacacgcgct
     9661 accgcctgac caagctcggc gaacaaatgg cgcgcctgcc catcgatccg aaaattgcgc
     9721 gcattttgct ggcggcgaag aaacacgact gcatggcgga aatattggtg attgcgtctg
     9781 cgctgtcgat tcaagacccg cgcgaacggc cgctggaagc gcgcgatgcc gcggccaagg
     9841 cgcatgaacg ctttaccgac aagcaatccg atttccttac ctacctgaat atttgggaca
     9901 gtttccagcg cgagcgcgac aaaggtttgt ccaacaagca gttggtgcaa tggtgccgtc
     9961 aatattttct gtcgcacctg aggatgcgcg agtggcgcga gctgcaccac cagcttgccc
    10021 aaaccgcgat tgaaatgggc ttgaccacca aagaagctgc gtttagacga cctcccgaag
    10081 tcaagcaact gacgtcgtct gagaatgcgg gcgaccaaga cctatctgcc aaactcaaac
    10141 aaaaacaact ggataagaaa caacaccgca cccaaatccg cgctaccaaa gaagcgggct
    10201 acgaacaaat ccaccgcgcc ctgctcaccg gcctcatcgc caacgtcggc atgaaatcgc
    10261 ccgacagcaa cgactacacc ggcgcgcgcg gcagccgctt ccaccttttc cccgcctccg
    10321 ccctgttcaa agccaagccc aaatgggtga tggcggcaga attggttgaa accacgcgcc
    10381 tttacgcgcg cgacgtcgcc gttatccagc ccgaatggat cgagcaggaa gccccgcacc
    10441 ttgtccgcta ccattatttc gagccgcact gggaacaaaa acgcggcgaa gtcgtcgcca
    10501 gcgaacgcgt aacgctttac ggcctggccg tattgccgcg ccgccccgtg tcttacggta
    10561 aagttgcgcc cgaagaagca cgtgaaatct ttatccgcag cgcgttggtg gcgcaggaat
    10621 acgatttgaa agcggatttt tttgtccaca acaaaaagct gattaaagaa attaccgaac
    10681 tcgaacacaa atcgcgcaag caagacgtat tggtcgatga cgaagccctg tttgcgtttt
    10741 atcacgaacg gctgcccgat ttttatatgg cggattcggt ttcagagggc ctgtgtcctg
    10801 caaatccgca gcaaaccacc ccctcccccg tgggggaagg ctggggagag ggcaaaacag
    10861 ttgccgcaca aaccaacttt tccgcaacct cagcaagccc tctccctaac cctctcccac
    10921 aggagaggga acagagtgcc tcagtttcaa cggtttcagg cagtctgaaa acaatgtctt
    10981 gcgaagcaag gctgaatttc tgcgaagcta aaactaaaac cgaaagcagc ctgcactccc
    11041 aaaggctatc tgaaaactac accccgccat tttcagacgg cctgcgtcct gcaaaccccc
    11101 agcaaaccac cccctccccc gtgggggagg gctggggaga gggcaaaaca gttgccgcac
    11161 aaaccaactt ttccgcaacc gcagcaaacc ctctcccaca ggagagggaa cagagtgcct
    11221 cggctttaac agtttcagat gaccccaaac ccaaaaagca gcctgcatct caaaaaggcc
    11281 gtctgaaacc tttgcccctt gccgatatcc gcacctttga agcctggctc aaaaccgccg
    11341 agcgcgacaa cccgcgcctg ttgttcctca gccgcgacga cctcatgcag cacgccgccg
    11401 cgcacatcac cgaagaacaa ttccccaaac actggcagac cgcagacggc aaattcaaac
    11461 tcagctaccg cttcgagccg caccacccgc tcgacggcgt taccctcacc ctgccgctca
    11521 ccgtgctcaa ccgcatcagc cccgccgccc tcgaatggct cgtgcccggc atgatacgcg
    11581 aaaaaatcca gctacaaatc aaagcactgc ccaaacaaat ccgccgcatc tgcgtacccg
    11641 tacccgaatt catcacccaa tttttaagcc aaaaccccga ccgcaacgcc cccatcctgc
    11701 cccagctcgc ccaagccatc gccaaaaccg caggcgacat ccgcatactc gagcaaatca
    11761 accaagacga atgggccgcg ttcaggctgc ccgaacactg ctatttcaac ctccgcatca
    11821 tcgacgacgg cggacaagag ttagccatgg gtcgcgattt aatccaaatc caacaacaac
    11881 ttggcaaagc cgccaccacc accttccgcg acaacaccca agaattcgag cgtgacaacg
    11941 ttaccgcatg ggacatcggc atcctgcccg aatccatcaa attcgcccgc ggcaaacaac
    12001 agctcaccgg ctacctcggc ctgcaaaaag aaaaagacgg ccgcatcgcc ctgcgcctgt
    12061 tcgacacgtc tgccgccgcc ggacacgccc acagattagg cgtgattgaa ctcatgaaac
    12121 tacaactaaa agaacaagtt aaagatctga acaaaggcat ccaaggcttc acccaagccg
    12181 ccatgctgct caaacacatc aacgccgaca ccctgcgcga cgacctcacc caagccgtct
    12241 gcgaccgcgc ctttatcggc gaagacaagc tgccgcgcaa cgaaaaagcc ttcaaagaac
    12301 aaatcaaacg cgcccgcagc cgcctgcccg ccgtcaaaga agccctcagc cgctacctgc
    12361 aggaaaccgc agccgcctac gccgaactga acggcaaact cggcaaacac ccattgaccc
    12421 acctcctaag actacgcctg caaaccctgc ttgcacccgg cttcgccacc cgcaccccgt
    12481 gggcacaatg gccgcgcctc cccatctacc tcaaagccat gaccctgcgc ctcgaaaaat
    12541 acagcagcaa ccccgcccgc gacgcagccc gcgaagccga tatccaagag ctggaacaaa
    12601 tgtggcagga aaaaactgac ggcttggcga aacaaggaca gcccgtttca gacggcctcg
    12661 ccgcgtttaa atggatgatt gaagaattga gagtgtcgct gttcgcgcag gagttgaaga
    12721 cgccgtatcc ggtgtcggta aagcggctgt tgaagatgtg
//
LOCUS       DAWTCI010000061        12651 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWTCI010000061 DAWTCI010000000
VERSION     DAWTCI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.061"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..628
                     /locus_tag="WM481_001594"
     CDS             <1..628
                     /locus_tag="WM481_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFA9080880.1"
                     /translation="TPRQILTAGAVVPVMAIDDLSTAVDLSRALVEGGIPTLEITLRT
                     PVGLEAIRLIAKEMPNAIIGAGTVTNPEQLKAVEDAGAVFAISPGLHESLARAGRNSG
                     IPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTGGISL
                     ATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(817..1002)
                     /locus_tag="WM481_001595"
     CDS             complement(817..1002)
                     /locus_tag="WM481_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080881.1"
                     /translation="MGYFGGNGGYLKRKQPKNLWLDVGCLKKRNFAKVSERYFLTTVN
                     LLVSAAHHGILPIGRFL"
     gene            complement(1013..1125)
                     /locus_tag="WM481_001596"
                     /pseudo
     CDS             complement(1013..1125)
                     /locus_tag="WM481_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1223..2263)
                     /locus_tag="WM481_001597"
     CDS             complement(1223..2263)
                     /locus_tag="WM481_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HFA9080882.1"
                     /translation="MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGI
                     WKGKNPEVADGRILGHEGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKIQLYS
                     HCRNGGWILGYMIDGTQAEYVRTPYADNSLVPPHDNVNEEIALLLSDALPTAHEIGVQ
                     YGDVKPGDTVFIAGAGPVGMSALLTAQLYSPAAIIVCDMDENRLKPAKELGATHTISP
                     ASGDVSKQVFAIVGEDGVDCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKL
                     EKLWIKNLAITTGLVNANTTEILMKAIFSSSVDYTKMLTHRFKFSELEKAYDVFKHAA
                     ENQAMKVVLEAD"
     gene            2426..3466
                     /gene="mutY"
                     /locus_tag="WM481_001598"
     CDS             2426..3466
                     /gene="mutY"
                     /locus_tag="WM481_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFA9080883.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VAAVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYGRARNLHKAAQQIVGQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFAFNRRETILDGNVKRVLCRVFAQDGNPQDKKF
                     ENSLWTLAESLMPSENADMPTYTQGLMDLGATVCKRTKPLCRQCPMADICEAKKQNRT
                     AELPRKKTALEVQTLPLYWLIVRNRDGAILLEKRTAKGIWGGLYCVPCFESLNGLSDF
                     AAKLSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSEHHSDGIWIKPGHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            complement(3842..4210)
                     /locus_tag="WM481_001599"
     CDS             complement(3842..4210)
                     /locus_tag="WM481_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080884.1"
                     /translation="MYDKIHKSCRFFAGSFCFMFGAVPDNRVKFPLAHYFKIEILYFI
                     SLLLKRCSHFFIGFIIHHLIYGFWRQGLTAFAAVIDFWNRGNAFKESKAIRPDADGGA
                     IIHLLEFVAIGADSCDDYVE"
     gene            complement(4228..6084)
                     /locus_tag="WM481_001600"
                     /pseudo
     CDS             complement(4228..6084)
                     /locus_tag="WM481_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(6122..6355)
                     /locus_tag="WM481_001601"
     CDS             complement(6122..6355)
                     /locus_tag="WM481_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFA9080885.1"
                     /translation="MGAGGSEAAAPIIGKWLYGKGDGGSLNAEEKETVSAITRMLGTA
                     AGAAEGNSSADAVWGCFQTASDFASSFSYPINM"
     gene            complement(6782..7475)
                     /locus_tag="WM481_001602"
                     /pseudo
     CDS             complement(6782..7475)
                     /locus_tag="WM481_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            complement(7990..12262)
                     /locus_tag="WM481_001603"
                     /pseudo
     CDS             complement(7990..12262)
                     /locus_tag="WM481_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            12463..12651
                     /locus_tag="WM481_001604"
                     /pseudo
     CDS             12463..12651
                     /locus_tag="WM481_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2963 a         3066 c         2961 g         3661 t
ORIGIN      
        1 gaccccccgc caaatcctga ccgccggcgc agttgtgcct gtgatggcga ttgacgactt
       61 aagcaccgcc gtcgatttgt cccgcgccct tgtcgaaggc ggcatcccca ccctcgaaat
      121 taccttgcgc acccccgtcg gactcgaggc catccgcctg attgccaaag aaatgcccaa
      181 cgccatcatc ggcgcaggta cggtaaccaa tcccgaacag ctcaaagccg tcgaagacgc
      241 aggcgcggtt ttcgccatca gcccgggttt gcacgaatcc ctcgccagag ccggccgcaa
      301 cagcggcatt cccctgattc ccggcgttgc cactccgggc gaagtccaac tggctttgga
      361 acacggcatc gacacactca aactcttccc tgccgaagtc gttggcggca aagcgatgct
      421 caaagccctc tacggacctt acgccgacgt acgcttctgc ccgaccggcg gcatcagcct
      481 cgccactgcg cccgattact tggcattgcc caacgtctta tgcgtcggcg gctcttggct
      541 gacaccgaaa gaagctgtaa aaaacaaaga ctgggacacc attacccgcc tcgccaaaga
      601 agcggcggca ttgaaaccca aagcctgaac cgcatcgtaa aaatgccgtc tgaagccctt
      661 tccagtctca gacggcattt tgccgcttgg cgactcagtc ggcatacaca gcagcaccgc
      721 ccctaaaatg cccatccatc tccagaacaa tgcgaatgac gcgcgatatg cggagaccta
      781 tgacgaaaaa ataattataa tatatttatc aattagtcat aaaaatcgcc ctatcggtaa
      841 aatgccgtga tgagcggctg aaactaacaa gttaaccgtc gttaaaaaat aacgctcaga
      901 gacctttgca aaattccttt tcttcaaaca gccgacatcc aaccacaggt ttttcggctg
      961 ttttcgtttc aaataaccgc cgttcccacc aaaatatccc attaatcccc cttaaatata
     1021 gtggattaaa tttaaaccag tacggcgtta cctcgccttg ccgtactatt ttactgtctg
     1081 cggcttcgtt gccttgtcct gatttaaatt taatccacta tacctgattg atcaggaaca
     1141 gcccgtttta cacggctgaa aaaatgccgt ctgaaacacg ttcagacggc atccgcttaa
     1201 aaaacaactg attcaacggc gattaatccg cttccaaaac cactttcatc gcttggtttt
     1261 cggcggcgtg tttgaacacg tcgtaggctt tttccaattc gctgaatttg aaacggtggg
     1321 tcaacatttt ggtgtaatcc actgagctgc tgaaaattgc cttcatcagt atttcggtgg
     1381 tgttggcatt gaccaaaccg gtagtgatgg caagattttt aatccacagt ttttccagtt
     1441 tgaaatcaac ggattgaccg tgtacgccga cgacggcgat atgaccgccg ggtttcacga
     1501 tgtcttggca catattccaa gttgcaggga taccgaccgc ttcgatggca caatctacgc
     1561 cgtcttcgcc gacgatggcg aaaacttgtt tggagacgtc gccggaagcg gggctgatgg
     1621 tatgggtcgc gcccaactct ttcgccggtt tcaaacggtt ttcgtccata tcgcacacga
     1681 taatggcggc agggctatac agttgggcgg tcaacaaggc ggacataccg acagggccgg
     1741 cacctgcgat gaatacggta tcgccgggtt tcacatcgcc gtattgcacg ccgatttcgt
     1801 gggcggtcgg taaagcgtcg ctcaacagca gggcgatttc ttcgttgacg ttgtcgtgcg
     1861 gcggcacgag gctgttgtcg gcataaggcg tgcggacgta ttcggcctgc gtgccgtcaa
     1921 tcatatagcc caaaatccag ccgccgttgc ggcagtgcga ataaagttgg attttgcagt
     1981 tgtcgcaagt gcagcatttg ctgacgcatg aaataatgac tttatcgccg actttgatgt
     2041 tttttacagc ctcgccgact tcttctacaa taccgatgcc ttcatgaccg aggatacgac
     2101 cgtcggcgac ttcggggttt ttgcctttcc aaatacccaa gtcggtaccg caaatcgtgg
     2161 ttttgacgat tttcaccacc gcatcggtcg gatcgataat ctgcgggcgg ggtttttctt
     2221 caaaacggat gtcgtttgcg ccgtgataaa ccattgcttt catgctgata ctccttgctt
     2281 gttgataaat aatttcaata ccacaataaa gtttctttat atgagttata tgcccctaca
     2341 aaaaataagt caataagaat tattttcaca attttataca ataacatact gttttaaata
     2401 taaataaaac caccgattga tattaatgaa cacacccatc cccttctccg aacggctcat
     2461 ccgctggcaa aaacaacacg gtcgccacca cctcccttgg caggtcaaaa acccttattg
     2521 cgtctggctt tccgaaatca tgctccagca aacgcaggtc gccgccgtgt tggactacta
     2581 tccgcgtttc ttggaaaaat tcccgaccgt tcagacgctt gccgccgcgc cgcaagacga
     2641 agtgttgtcg ttgtgggcgg gcttgggcta ttacggccgc gcgcgcaatc tgcacaaagc
     2701 cgcgcaacaa atcgtcggac aattcggcgg tacgtttcca tcggagcgca aagacttgga
     2761 aacgctctgc ggcgtaggca gaagcaccgc cgccgccatt tctgcctttg ctttcaaccg
     2821 acgagaaacc atcttggacg gcaacgtcaa acgcgttctc tgccgtgttt ttgcccaaga
     2881 cggcaatccg caagataaaa aatttgaaaa ctcgctctgg acacttgccg aaagcctgat
     2941 gccgtctgaa aacgccgata tgcctactta cacgcaaggt ttgatggatt tgggcgcgac
     3001 cgtgtgcaaa cggacgaaac ccttgtgccg tcaatgccct atggcggaca tctgcgaagc
     3061 aaaaaagcaa aaccgcaccg ccgagctgcc gcgtaaaaaa actgccctcg aagtgcaaac
     3121 cctgccgctt tactggctga ttgtccgcaa ccgggacggc gcaatcttgc tggaaaaacg
     3181 caccgccaaa ggcatttggg gcggactgta ttgcgtgccg tgttttgaaa gtttgaacgg
     3241 gctttccgac tttgccgcca aactctccct gacgatggcg gatatggacg aacaaaccgc
     3301 cctgacccac cgcctgacgc accggctgct gatgattacg ccgtttgaag ggcaaatgcc
     3361 gtccgaacac cattcagacg gcatttggat aaagccgggc catttgaaag attacggcct
     3421 gcccaagcct ttggaaattt atttaaacgg taataggtta gaataaacaa aataaacccg
     3481 ttgaactgtt gttagcaggt atcgcagcaa gaacaaccga tgaatttggg tcgtatttta
     3541 ggcagcggga taatgttcaa atgggacatt tggaacggaa aaagtcggca atttaaaaag
     3601 gattttagaa acaaagaggg tcaaaaacat gaacacaaac ttaaatgaca aagacaaagc
     3661 catggatacc gcaatcaggt ttcagaaaag gatgaggatt ccgaaatttt tctttttaat
     3721 tctcggaatc acagtggttt tggcatttat ccaagacgtg ataacgggtt ctaattttct
     3781 gcaaataaca attatgtaaa taatatttgt gatatatttc agtttcggag gtatcataat
     3841 tttattctac ataatcatcg caggaatctg cccctattgc cacgaattcc aaaagatgaa
     3901 ttatggcacc tccgtcggca tcgggtcgga tagctttact ttctttaaag gcatttcccc
     3961 tgttccaaaa atcgattact gcagcaaatg cggtaagccc ctgtctccag aagccgtaaa
     4021 taaggtgatg aatgataaaa ccgatgaaga aatgtgaaca gcgttttaac aacagtgaaa
     4081 taaaatataa aatttcaatt ttaaaataat gagctaatgg aaatttgacc cggttgtccg
     4141 gtacggcccc aaacataaaa caaaagctgc ctgcaaaaaa ccggcagctt ttgtgtatct
     4201 tgtcatacat tattcacttc aaccgcatca aaacgaataa cccacctgaa accccgttac
     4261 ccatttcttc gtctgaaaat attcgggctt tttcaatgca cggccggtaa atatatcgta
     4321 atgcaggttg ccgccaagct ttatctgccc gcgtatccca attgctgtgc cggctagagt
     4381 ttggcccgat aaccatttgg cggattgtcc tgaaacatgt cctacatcag ccccaagata
     4441 aagctgatgg cctggtttaa attgccagct caaatcgttg cgccaatacc atccccgctc
     4501 ggcaggcaaa ctcatttcac cgtcgaagcc acgtacggtg tggtgtccgc cgatagccag
     4561 tttgtcttgc gatgttagcg gggttttgtt ccattgtgca tgaacggatg tgtcataggc
     4621 aaatagctgt ttaccgattt gaaaaggagt atttacatca gccgatgccg tccaaatttt
     4681 catacgtgac gtgccttcgc caaaggcttc ttcaggcgcg cgcagagcat ctttcatgcc
     4741 ggtgccgtgt ttatatttca acttaaaatc tgccgtactg cgaccgatat atcctttgtg
     4801 ggaaagttct gccaaccaac ctgtggtttt acgccgttgt acagtcagtt cggcatcatc
     4861 aatgtaactt tttgtttccc tcgtccacag ttttacactg agataggttt tgcgtttggc
     4921 atcacgatac aacaggcggt tgaagccgaa atcagtgttg taactttttc cattatagtc
     4981 atagacttcc gataatccgg aaaccgcctg atggtaacgg tagccattgt gattgaatgc
     5041 ccatgtccat ttaccgaaag gggctgaata atgtacggcg taattgtttg atccgccttc
     5101 tttgcgatgg ccgtcaaaat tttcctcatc gggcgtaccg ccaattgaac gtccataatt
     5161 tacatagaac atatcactca gtccaaaagg attgtcggca gagaaagtga tatttccttg
     5221 gtattttcct gtcgcctcac tacccgaatt atccatcccc acactcacac agtagggcag
     5281 cagacgttac cgccattgca ccacgacatc actttggttt ggttctctct ctacgggaac
     5341 gatttggaga tcggcttccg cagtcgggag acatttgaga ttttccagtc cttgttccaa
     5401 atcacgcaga ttcaacagat cgttcgagcg ggtgggaaat ttgttttgga atgctgcaat
     5461 acgtcctgca tgggtttgat catcgttgga ccgatcgatt cgtatggagc gcagatagcc
     5521 cggcatcagg gttaattgaa gcttgccact attcaaatcc tgtggcgcag ccaagatacg
     5581 ggtcgtggta tatcccctgc cgatcaaagc attttgtgct aaggacatga tttgattaat
     5641 gtcgcccgca tgcagacact tgccggaaac aaaatgtgtt tggcacaagg catggttgag
     5701 cgcaaaccgg aatttagccg cttcttcacc caccaattcc acctcagaaa taggaaagca
     5761 ggggcttgcc tcatccgaat ttgcaccgcc tacctgtgtt gccatcttct ctatgcccgt
     5821 atcggtacca tccaaacgaa catcttgttc cggctgcatc tgttcacgca attgcttctc
     5881 acgttgacgt tggagaatat cctgttgttg tgaaacagct tcaggtgtgg gggcggctga
     5941 tgttagggtg gggaggcagc catcaatgat aggactaaca gtgataaggg atagggatga
     6001 aatacatttt tggaggaaaa gaaaggtttc ataggattgg tctaagtaat gtaagcgggc
     6061 aattatagca atgcacaaaa acaaacaaaa agataaaaaa actccataaa tgaaaattta
     6121 atcacatgtt tataggatat gaaaaagagg aagcgaaatc tgaagccgtc tgaaaacaac
     6181 cccacacagc atcggcggac gagtttccct cagctgctcc ggcagccgta cccagcatcc
     6241 ttgtaatcgc cgaaacggtc tctttttcct ccgcattcag gctgccgccg tctcctttgc
     6301 cgtacagcca tttgccgatg attggggcgg ccgcttccga cccgcccgca cccaatgctc
     6361 ctgccggggc attgttgcct catgctgcgg caaccgctgc tcctaatacc gcgtgggcaa
     6421 gaacgtgagc ggtttcttga ctggcggtta gtttgccatt cgcgttttga ccggctaaat
     6481 ctttaaagtg ctgtccaatc gcatacgata cgtctggcga tgcggtagcc gcagcgatgc
     6541 ccgctccgct ttgggtcggc tcagctaaac ctgaggctaa catgttgaga atgactttgc
     6601 cttgttgcca attatctgct tttgctgccg catcttgagc ttcatgggct ttgcgtttgg
     6661 cagtttccat atcgccattg gctaatgcct cggctgctgc tgtttcggct gcttctttgt
     6721 ctgctttgag tttgtctaaa tgttggttaa tctcggtatt ggtttgttga acatttttac
     6781 taaaatcttg gctgacggtt ctttgtaaat ccagttcact ttgcactcta tctttgtcaa
     6841 atatgttttt caggctgccc gaatgtcgtt cggcggtgtc tgtggttacg tttgtatcaa
     6901 tatcggcttt ggtttgtgcc gctattttgc ctgtcagcct gatttgtgcg gcttcgtctg
     6961 tgatttgaat gtttttgggt attgatgccg ctttttgtga tgctgctttg actgtcgctg
     7021 tcgctgccgt accctactga tgaacttgcg ccgtttttat cggctacgct tgtcaggtgt
     7081 ttgtcttgag gtttattttt tgcgccctgt cccagtgttt tgccgcttac ggcaacgctg
     7141 gcacccaatc caaaactttt tccttcgtat tggctgtaat tttgaatatc actgccggcg
     7201 agtgtgcctg tgctgaatcg gtttttaccc ttgtcttttg cgctttggct gctggtgatg
     7261 atgccgcctt tgaggcctgt atggtttccg accttgattt gatagccgtc ttctccggca
     7321 taaataccgc tttgctcggt taccgaagca tggtcggctc ggattttgct ttggctgtaa
     7381 tcgccactgg cactgaagcc ataacctacg gtaacttgtg caccggcgtt ttgttgtttg
     7441 ctttgataag tttctctatc ttgtacactt tgaatgctta ggtttttggc attgacttgt
     7501 acgcctttgc cgcgtacttg cgcgcctttg atgatggtat cgccaccact ttggataagg
     7561 gtttggctgc ctttgtcgcc aatatggcta tggcggtggg ttacgctatc gccattgccg
     7621 tagcctttgc cgacattgcc gcctgcggca acgcctaatg accagcctcc ttgtccgaat
     7681 gatacggcag cacctgcgtt ccagcctgct gatttgtttt ggctgcgttc ggtattgctt
     7741 tgctcggctg attgcagatg gatttgctta tcggcaatca ggccggttcc tgctctgcct
     7801 gaaacacctg agcctgtgat gttgatattg gattgttcgc cgcaccttcg gcaataaggg
     7861 tagttttgcc gcctgcttga atttgactcg cttgggcttg attggcttga acttgggtgg
     7921 tttgtcggtt ttgctgttcg ccgtaggtta tggagatgct gacttgtttg gcattggttg
     7981 taccattggc taagttttgt gcgcctttgc ctgtttgata ggcctgccaa cctgcattgg
     8041 cagccgccat ggcattaacg cggtcgtttt tgctttgtcc gacttgtttg ctgctttgtg
     8101 tgctacggca atcgcttgtt gtgccaaatc ggtaacgggc gaactgaatg ccaccgttaa
     8161 gcctttttgt tcgtaggttt gggtggtttt gctgtttaat tggttttgtg ctgcgccaat
     8221 atccatactt tgcgtgctga taaggttgtt gccctcaggg ctggaaacgt tgctgccggt
     8281 ttgttcgtag tgtttgcttg caacaatggt ggtatcgcct ttcaggctgc ctacggtact
     8341 gcctgtatgt tcgttgcttt gggattggtt ttcttgtgtg tttgtcttgc tgccaatagt
     8401 gaagccgata cctgcactca tcaatcctga tttttgggtt tgatgatagg tttcgctttg
     8461 gctttgagtt tgggttgtac caatgcgaac atgattgcct gcttgaatcc gggtgccatt
     8521 atcggaaata acattactgc caaggatgtt ggcatcgttt cctgcctgca atacaacttg
     8581 cttgccttca aaggtgctgc tttgagcagt ttcgtgatga ctttgggctt tatcggtaat
     8641 gactaattta ttaccgccgc cgcttctgcc tgtatgtttg gacgcatcat caacttggcc
     8701 ggcatggatg cctgagctga tagtaatgtc atttttagca tacacggcaa gtgtgccttt
     8761 tgcgctgccg acttcggcag ctttggcatt gagattattc cctgacaata gggtaacatc
     8821 gccttttgtt tgaatgctgc tgccgacttc gttcgttgaa cctcggatgg tatggttatc
     8881 ggcatcaaaa tggatttctt gatatttgcc ggtttgtacc gtatccaggt taatgtcgcg
     8941 tcctgcctgc agccgggttt gcccttgatc tgattgattg ctgatttgac cggcaatgat
     9001 gttgatgtct ttgcctgcct gcgctgctaa aacacctttt tctttgcctg tgatataaat
     9061 acctgccatt cggtctaggt aggtgctgct accttgtgca ttttgactgc tcttggccgt
     9121 gctttggttg ttgatgttgt tacccgcatt gagcaataat gtctgttcgg cagaaagaat
     9181 gccgccaata ttattgatgt cttgtgtggc cgtaaccgct gatttttgcg catgaatacg
     9241 cccaccgata ttgtctagcg tatcggtatt gataataagc gcattgcgcc ctgcaatcgt
     9301 gcctgagttt ttcaggctgc ctgaaacatt gatttgtgta ttgctgcctg acaacaatgc
     9361 acctttaccg tctatgccgc catttttaac gcgtacataa acctgtggca tcaatacggt
     9421 ttgtgtgccg ccatcaggaa gtttaacttc tttttgtacc aaccaaacaa tatcgctggt
     9481 cagttgcgct gcttgctcgg cacttaatgc aatgccaacg ctgagattca tcgaacgtgc
     9541 cgcagtcgcg ccattatcca ttaaggcttt aaattgttct tcgtcgtttt gataaccgtc
     9601 taaacgacga tgccctgtca gctctgcgat ttgttcattg attaaacgtt gctcgtaata
     9661 accatcaccc aaacgtttat gtaaattgtt tgggtctagt ttgaggctgc ccagcatata
     9721 gtcactaccc aaccattgac ggtagttggc aaagcgtgga tcggtttcaa caagatagcc
     9781 tttattggca ggattgataa tgtataagct gctgccgggt aatggggtaa aagaattggg
     9841 cgtatagggt agcgaaatac cgttgctttt cgcagtacgg atattatccc ggttactttg
     9901 tggcaactca gtgccttggc tgggcgcatg acggcttaat gctttgctat gcgattcata
     9961 ggcaaatgaa cccagtgaaa tgtcgcgtgt gatttcctcc ggcaaagtat aattttgttc
    10021 acgatgccct gtttcatcat gtcctttacg acgcgcacgc cagtagttgt gcaacttacc
    10081 attttcgctg aagactttct tctcgccaaa ggtttgctcg ttatgcaaac cgtctttttc
    10141 tgtttgcaca agcaaattgc cgccggcgat gattcggctg tcgctgttga agactgcttt
    10201 gctatcaata atcaaatcgc tacctgcaat gattttagca ggcgcagttc cggttacttg
    10261 agtttcttgc gttacttttt cataatcgta tttatgccaa ttttcatgcg ccactccatc
    10321 aggggtgcgt aagtggtctg attcattgtt gtagacaaac cagcctaatt catgttgcgt
    10381 gccttctcgc aataattcgt gtcgtccaaa tgcttcgtaa tcaacaatac gctcgcgccc
    10441 tgtttctacc aactgcgttt tcaaatgctc attggtattg tgcagctttt ctacacctaa
    10501 acgcattttg cctgcagctt caatgattgc gccggcattg tgtatccttt gggctttgcc
    10561 tgtggcttgg tcattggtat ctaatgcgcc gccaaccgcc atatcgttac cgctgtaaat
    10621 cagactgttt tcacggttat ttaattgttc aatgcctaaa ttcaggtttt cgcgtgccgc
    10681 aatggcggca cctgtaccgt tttcatcttg attgtctaag cgggtagccg caatagcgat
    10741 attgtcgcca taaatccgac ctgtaccgat attattcatt tgcccggctt ggattttggt
    10801 ttgttgtccg tcaatcaagc ctctattggt taaattgtgc tgcgtgccaa tgtctgtcgt
    10861 accgccggat tgaatgttgc cttgtactgc attatcaagg ttatttgctt taatccgaat
    10921 gcgttttcct gcttgcaagg tatgtgaatt tttcaggctg cctcgtgtac tgagcgacaa
    10981 ttcattgccc gccacgatct tgcgttctac ataaaaatca tcttgtaacg caatatccag
    11041 tttattatca gcggcaagtg tgccgttgtt ggataacgat tttgcctgaa tggcaacatc
    11101 acggcctgat tgtatcgtgc cattcgtatt atcaatgaca acggtagatt gctgaccatc
    11161 gtgaataatc agttgttgat tggtcgctat ttcgccattt tgattgttca ggctgcctga
    11221 aacggctaaa tcctctgttc ctgccgacaa taatttgcca gtgttttgaa tcgattgact
    11281 gtgaatttga gtgccttgtt gcgataccgc cgtaccgctg ttttcaaagg cttgactgcg
    11341 gatattgact ttgtgttccg ctgtattatt catatcttgc acattggcgg cagccatcgt
    11401 gccactattg accaaacggc catttgcatc aatcgccaca ttaccggaag aagcaaacaa
    11461 ctgcccttga ttacgaatgc ctgcttgttc ggccgtactg atcaaggtga ttttgttggc
    11521 atacaagaat acgtgtgaaa tcggtatcac gggcatccaa accgtgtccg gcgattacaa
    11581 cattgccttg ccttatctta aagccgctaa agtctcctgc ttgatattgc ggttggcctg
    11641 tcgtcaaagt ggcacgggaa gcattgataa aaccaccacc attgactgca atccctgccg
    11701 gattggcaat aacgacttct gcacgtcgtc cacccacttc aatatagcca ttcagttgtg
    11761 aaggatggct gctgttgatt tggtttacaa ccacacgtgc ttcgcccctt gtcaaccaag
    11821 gattgccttg aatccaaccg cctagctgtg tttgggtgtt gctgcgactg ttgtttaaaa
    11881 tcgccccgcg attacccaca tcaaactggg catattgatt aacagaaacc cctgccgaag
    11941 taggggtttg aatattgact tgcggtatgc cgttacctgt ttgcagaatc gtggcttgtt
    12001 gggttttagg agcagcttta tcagtaataa tgccgtcagc aaaagcaata ttgaccgtac
    12061 ccaaagccaa acataaagaa aagcctaatg cagaaaaaca aaaggctttg gaatgagtag
    12121 gaatgaaaga aacggatttc acataaacgc tgcccgaacc actatcggca cagcttttac
    12181 cttcgcgctt ggtggtttcg gcaacagcta ccacagcacc gcgtttgcgg ttgaaaatca
    12241 cacgatagag ggttttgttc atgattttag ttatttgatt tttatagagt tattacaaac
    12301 attggacagt ctgggcattc taggacaaag attttggtaa gtcaattacc gccatttttt
    12361 tattaaatta ggggacgtac cggataacgg ctaaaaatcc catttaggtt agaataagcc
    12421 aatgagaaaa gccgtctaag ccggcataaa caaaataaac tcattgggct atttgtcgca
    12481 ggcgtaactg caagaacagc ggcagagttg gtaggcgtta ataaaaatac cgcagcctat
    12541 gattttcatc gtttacgatg actgatttat caaaacggtc cgcatttaga aatgtttgat
    12601 ggcgaagtag aagcagatga aagttatttt ggcggacaac gcaaaggtaa a
//
LOCUS       DAWTCI010000062        11942 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWTCI010000062 DAWTCI010000000
VERSION     DAWTCI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11942
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.062"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            1..486
                     /gene="def"
                     /locus_tag="WM481_001605"
     CDS             1..486
                     /gene="def"
                     /locus_tag="WM481_001605"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009118779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFA9080886.1"
                     /translation="MQYPDERLHTVAKPVEQVDERIRKLVADMFETMYESRGIGLAAT
                     QVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDAVTRAERVK
                     VEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLKKRQKHT
                     I"
     gene            574..1500
                     /locus_tag="WM481_001606"
     CDS             574..1500
                     /locus_tag="WM481_001606"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFA9080887.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     ADIIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGTAGEVLSCSADGLVVACGES
                     ALKITELQPAGGRRMNIAAFAAGRSIEAGAKL"
     gene            1579..2838
                     /gene="rsmB"
                     /locus_tag="WM481_001607"
     CDS             1579..2838
                     /gene="rsmB"
                     /locus_tag="WM481_001607"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFA9080888.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAHIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLREREKLAASCKKDDVAKYNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAVEGIAAKALDEYAVTLEEAVPVNRL
                     PGFAEGLVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARRQEALLDALWQALKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            2804..3403
                     /locus_tag="WM481_001608"
     CDS             2804..3403
                     /locus_tag="WM481_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFA9080889.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIAATRAEARITDG
                     GRLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPTIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            3403..5523
                     /locus_tag="WM481_001609"
     CDS             3403..5523
                     /locus_tag="WM481_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFA9080890.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVSFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALDLAADNAVSNAVPVQIDLIGTASLSGNMGSVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPIPENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTL
                     LIASLLSIFLALVMALYFARRFVEPILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLDGLTTGVVVFDEKGRLKTFNKAAE
                     QILGMPLAPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVQVEYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIRAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDDQDAQILTRSTDTIIKQVAALKEMVEAFRNYARAPSLKLENQDLNALI
                     GDVLALYEAGPCRFEAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADMPEVRVK
                     SETGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIGEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            5516..6793
                     /locus_tag="WM481_001610"
     CDS             5516..6793
                     /locus_tag="WM481_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9080891.1"
                     /translation="MRSSDILIVDDEVGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVVMSGHASIDTAVEATKIGAIDF
                     LEKPISLQKLLSAVENALKHGAAQIETGSVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGITFIIGKAERCRVRVIASCSYAAGSDGISCEEKLAGLFSESVVRIPPLSMQHE
                     DIPFLIQGIACNVAESQKITPTAFSDDALAVLSRYDWPGNFEQLQNVVTTLLLEADGQ
                     EIGIGAASSALGRGVPADGTGHMACGFDFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            6884..8071
                     /gene="dprA"
                     /locus_tag="WM481_001611"
     CDS             6884..8071
                     /gene="dprA"
                     /locus_tag="WM481_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFA9080892.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRSFGSAQNALSAPAEQV
                     APAVRHKHALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPTVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPANKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEG
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            8097..8558
                     /locus_tag="WM481_001612"
     CDS             8097..8558
                     /locus_tag="WM481_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFA9080893.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPAGSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            8636..10942
                     /gene="topA"
                     /locus_tag="WM481_001613"
     CDS             8636..10942
                     /gene="topA"
                     /locus_tag="WM481_001613"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFA9080894.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAAAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDGKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRRWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELNETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLAYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRRGVEKVCPQKECGWKEQIEPPAPQE"
     gene            11190..11825
                     /locus_tag="WM481_001614"
                     /pseudo
     CDS             11190..11825
                     /locus_tag="WM481_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         2936 a         3294 c         3395 g         2317 t
ORIGIN      
        1 ttgcaatatc ccgacgagcg tctgcacacg gtggcaaagc ctgtcgaaca agttgacgag
       61 cgcatccgga agctggttgc cgatatgttt gaaacgatgt acgaatcgcg cggcatcggg
      121 ctggcggcga cgcaggtcga tgtgcacgaa cgcgtggtcg tgatggattt gaccgaagac
      181 cgcagcgaac cgcgcgtgtt catcaacccc gtcatcgttg aaaaagacgg cgaaaccact
      241 tacgaagagg gctgcctgtc cgtaccgggc atttacgacg ccgttacccg cgccgaacgc
      301 gtcaaggtcg aggctttgaa cgaaaaaggc gaaaaattca cgctggaggc ggacgggctg
      361 ctggcgattt gcgtgcagca cgagttagat cacttgatgg gcatcgtgtt tgtcgaacgc
      421 ctttcccaac tcaagcaggg gcggattaag accaaactga aaaaacgtca gaaacatacg
      481 atttgattcg cttcgtcatg ccgtctgaac gctgcaaggc tttcagacgg cacagccttg
      541 tccgacaact ttacgcacac gcaggaacac gctatgaaag tcatctttgc cggcacgccc
      601 gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat cccgctggtg
      661 ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc gcctgtcaaa
      721 caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg caacaacgcc
      781 gaagccctgc aaatgctcaa agaggtcgag gcggacgtaa tggtggttgc cgcgtacggc
      841 ttgattctgc cgcaggacgt gttggatacg ccgaaacacg gctgcctcaa tatccatgct
      901 tcgctgctgc cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggccggcgat
      961 gccgaaaccg gcgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc
     1021 agcgaacacc gctacgccat ccaacctacc gatactgcca acgaagtcca cgacgcgctg
     1081 atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt
     1141 ctgaacgcgg tcaaacagcc cgaagaaggc gtaacttacg cccaaaaact aagcaaagaa
     1201 gaagcgcgta tcgattggag cgaaagcgcg gacattatcg aacgcaaaat ccgcgccttc
     1261 aaccccgtgc ctgccgcgtg ggtcgaatat cagggcaagc cgatgaaaat ccggcgtgcc
     1321 gaagtggtgg cgcaacaagg tacggcaggc gaagtgttgt cctgttcggc ggacggtttg
     1381 gtcgttgcct gcggcgaaag cgcgctgaag attaccgaat tgcagcctgc cggcggcagg
     1441 cgtatgaata ttgcggcgtt tgcggcggga cggtctattg aagcaggggc gaagctgtaa
     1501 atcccttcag acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagttgaa
     1561 gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcatt
     1621 gcggcggttg ccgaagggcg caaccttcag gacgtgttgg cgcacatccg cgcggcgcat
     1681 ccgcagctga cggcgcagga aaacggcgcg ttgcaggata tcgcctacgg ctgccagcgt
     1741 tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaagcccat cgacaatccg
     1801 cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg caacgcgccc
     1861 cacgccgtgg tcaacgaagc tgttgaaagc atagccaaaa tcgggcgcgg gcagtaccgt
     1921 tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gtgaaaaatt ggcggcttcc
     1981 tgcaaaaaag acgatgtggc gaaatacaac ctgccgctgt ggtgggttgc ctacctgaaa
     2041 aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca tccgccgatg
     2101 acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa actggctgtg
     2161 gaaggtatcg cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg
     2221 gtaaaccgtc tgcccggttt tgccgaagga ctggtgtcgg tacaggactt cggcgcgcag
     2281 caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc gtgcgccgcg
     2341 ccgggcggca agacggggca tattttggaa ctggcggact gccgcgttac cgccttggac
     2401 atcgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg ctttcagacg
     2461 gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc
     2521 gacgcaatcc ttgccgacgt gccgtgcacc gcttccggcg tggcgcggcg caatcccgac
     2581 gtgaaatggc tgcgccgacc gaccgacgcg ctcaaaaccg cccgccggca ggaagccctg
     2641 ctggacgcac tttggcaggc gttgaaacag ggcggcagaa tgctgcttgc cacctgttcc
     2701 gtgttcgtcg aagaaaacga cgggcaactg caaaaattcc tcaaccgcca tgccgatgcc
     2761 gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg
     2821 cttattcaaa agcattaaac aatggcttgt gctgttgccg atgctctccg ttttgccgga
     2881 cgcggcggcg gagggcattg ccgcgacccg cgccgaagcg aggataaccg acggcgggcg
     2941 gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg
     3001 ccggggcgta ccgctcaact ttaccttaag ctggcagctt tccgccccga caatcgcttc
     3061 ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag
     3121 tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcattttcca ccgattacga
     3181 cactttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa
     3241 aggcgcgttg tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc
     3301 cacttcaaaa ctgcccaagc ctttccaaat caacgcattg acttctcaaa actggcattt
     3361 ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg cttcctaccg
     3421 atcgcagcca tatgcgccgt cgtcctgctg tacggattga cggcggcaac cggcagcacc
     3481 agttcgctgg cggattattt ctggtggata gtctcgttca gcgcaatgct gctgctggtg
     3541 ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgtg
     3601 ttcggttcgc agattgccaa acgcctttcc gggatgttca cgctggtcgc cgtactgccc
     3661 ggcttgttcc tgttcggcat ttccgcgcag tttatcaacg gcacgattaa ttcgtggttc
     3721 ggcaacgaca cccacgaagc cctcgaacgc agccttaatt tgagcaagtc cgcactggat
     3781 ttggcggcag acaatgccgt cagcaacgcc gttcccgtac agatagacct catcggcacc
     3841 gcctccctgt cgggcaatat gggcagtgtg ctggaacact acgccggcag cggttttgcc
     3901 cagcttgccc tgtacaatgc cgcaagcggg aaaatcgaaa aaagcatcaa tccgcaccaa
     3961 ttcgaccagc cgcttcccga caaagaacat tgggaacaga ttcagcagac cggttcggtt
     4021 cggagtttgg aaagcatagg cggcgtattg tacgcgcagg gatggttgtc ggcaggtacg
     4081 cacaacgggc gcgattacgc gctgttcttc cgccagccga ttcccgaaaa tgtggcacag
     4141 gatgccgttc tgattgaaaa ggcgcgggcg aaatatgccg aattgagtta cagcaaaaaa
     4201 ggtttgcaga ccttttttct ggtaaccctg ctgattgcct cgctgctgtc gatttttctt
     4261 gcgctggtaa tggcactgta ttttgcccgc cgtttcgtcg aacccattct gtcgcttgcc
     4321 gagggcgcaa aggcggtggc gcagggtgat ttcagccaga cgcgccccgt attgcgcaac
     4381 gacgagttcg gacgtttgac caagctgttc aaccatatga ccgagcagct ttccatcgcc
     4441 aaagaagcag acgaacgcaa ccgccggcgc gaggaagccg cccgccacta cctcgagtgc
     4501 gtgttggatg ggttgactac cggtgtggtg gtgtttgacg aaaaaggccg tttgaaaacc
     4561 ttcaacaagg cggcggaaca gattttgggg atgccgctcg cccccctgtg gggcagcagc
     4621 cggcacggtt ggcacggcgt ttcggcacag cagtccctgc ttgccgaagt gtttgccgcc
     4681 atcggtgcgg cggcaggtac ggacaaaccg gtccaggtgg aatatgccgc gccggacgat
     4741 gccaaaatcc tgctgggcaa ggcgacggta ttgcccgaag acaacggcaa cggcgtggtg
     4801 atggtgattg acgacatcac cgtgctgata cgcgcgcaaa aagaagccgc gtggggtgaa
     4861 gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc
     4921 gaacggctgg cgtggaaatt gggcgggaag ctggacgatc aggacgcgca aatcctgacg
     4981 cgttcgaccg acaccatcat caaacaggtg gcggcgttaa aagaaatggt cgaggcattc
     5041 cgcaattacg cgcgcgcccc ttcgctcaaa ctggaaaatc aggatttgaa cgccttaatc
     5101 ggcgatgttt tggccctgta cgaagccggc ccgtgccggt ttgaggcgga acttgccggc
     5161 gaaccgctga tgatggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc
     5221 aaaaatgccg ccgaagcggc ggaagaagcc gatatgcccg aagtcagggt aaaatcggaa
     5281 acggggcagg acggacggat tgtcctgacg gtttgcgaca acggcaaggg attcggcaag
     5341 gaaatgctgc acaatgcttt cgagccgtat gtgacggata agccggcggg aacgggactg
     5401 ggtctgcctg tagtgaaaaa aatcattgga gaacacggcg gccgcatcag cctgagcaat
     5461 caggatgcgg gtggggcgtg tgtcagaatc atcttgccaa aaacggtaga aacttatgcg
     5521 tagcagcgat attttaattg tagacgacga agtcggcatc cgcgacctgc tgtcggaaat
     5581 cctgcaggac gaaggctatt cggtcgcgtt ggcggaaaat gccgaagagg cgcgcaagct
     5641 gcgccatcag gcgcgcccgg cgatggtgct gctggatatt tggatgcccg attgcgacgg
     5701 tatcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtggtgat
     5761 gagcgggcat gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgatcgattt
     5821 tttggaaaaa ccgatttccc tgcaaaagct gctgtctgcg gtcgaaaacg cgttgaaaca
     5881 cggtgcggcg caaatcgaaa cgggatcggt gttcgacaaa ctgggcaaca gtgccgcgat
     5941 tcaagagatg aaccgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac
     6001 gggcgaggcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc
     6061 gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa
     6121 ggcggagggc ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac
     6181 cggcattacc tttatcatcg gcaaggcaga acgctgccgt gtcagggtga ttgcttcgtg
     6241 cagctacgcg gcaggttcag acggcatttc atgtgaagaa aaattggccg ggcttttttc
     6301 ggaatcggtc gtccgtattc cgcccctgag tatgcagcat gaagacattc ccttcctgat
     6361 acaggggatt gcctgcaatg tggcggaaag ccaaaaaatc acgcctaccg cattcagtga
     6421 cgatgcgctt gccgtattgt cccgttacga ctggccgggg aatttcgagc agttgcaaaa
     6481 cgtcgtgaca acgctgctgc tggaggcgga cgggcaggaa atcggtatcg gcgcggcttc
     6541 ttcggcactg gggcgcggtg tgcctgccga cggaacgggg catatggcgt gcgggttcga
     6601 ctttaacctg ccgctgcgcg aattaaggga ggaggtggag cggcgttatt tcgagtacca
     6661 catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac
     6721 gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa
     6781 aaccgaagaa taggcccgga cggccggttt cccggctgcg ggcttttgtt ttcagacggc
     6841 atttggagaa aatgccgtct ggaatcgtaa ggggatggat tttatgacag aggacgaacg
     6901 tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagtt tcctgctgct
     6961 gatgcgcagt ttcggcagcg cgcaaaatgc cttgtccgca ccggcggaac aggttgcccc
     7021 ggcggtgcgg cataagcacg cgcttgaggc ttggcgcaat gcggaaaaac gcgctttggc
     7081 gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gcctgatgct
     7141 gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt
     7201 gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg
     7261 ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa
     7321 aggcattcct accgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc
     7381 actggaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc
     7441 gccggccaac aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg tcagcgagtt
     7501 ccccatcggc acgcggccgt atgccggcaa ctttccgcgc cgcaaccgcc tgattgccgc
     7561 gctgtcgcag gttacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc
     7621 cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga tagacaaccc
     7681 ccacagcaaa ggctgccaca aactgattaa agacggagcg aaactgacgg aatgtttgga
     7741 cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcgt catattctat
     7801 aaataaggat acgcccgaca cgggccgccg cgccgttcag acggcatacg ccccgccgcc
     7861 tgccgcaaaa atgccgtctg aaggggcggc aggcggcaca gccgtcggcg gcatactgga
     7921 taaaatgggt ttcgacccca tccatcccga cgtgcttgcc ggacagttgg ctatgcctgc
     7981 cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg ccgccatgcc
     8041 cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga acacgaatga
     8101 ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgc ccgccgcccg
     8161 aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc ggcaacaccc
     8221 tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc gccggcagcg
     8281 gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa gtgatggggc
     8341 tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa atcatcatcc
     8401 acgcgctcat gcacatcccc ggcgacgaaa ttaccgtaga taccgccaaa gtgctgaccc
     8461 tgctgctttt atgggcaaac aagagcgagc tgcccgtatt ggtcggcgac gagctgatga
     8521 gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga cggcgccggc
     8581 ccgcccgatg ccgtctgaaa caccggcgtc aaaaccacca tccagagaac gacaaatggc
     8641 gaaaaacctc ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg
     8701 cggcgatttt gaaatcctcg cgtcctacgg acacgtccgc gacctcgtcc ccaaaagcgg
     8761 cgcggtcgat cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa
     8821 acatgtcgat gcgattgttg ccgctgccaa agaagccgaa aacatctacc tcgcaaccga
     8881 cccggacagg gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg
     8941 cggcctgaaa aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt
     9001 gctcgatgcg gttgcccatc ctcgcgaaat cgaaatggat ttggtcgatg cgcaacaagc
     9061 ccgtcgcgct ctggactatc tggtcggttt caacctctcg ccgttgctgt ggaaaaaaat
     9121 ccgacgcggt ttgagcgcgg gccgcgtgca aagccctgct ttgcgcctga tttgcgaacg
     9181 cgaaaacgaa atccgcgcgt ttgaagcgca ggaatattgg acggtacatc tagacagcca
     9241 caaaggccgc agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca
     9301 attcgacctg ccgaacgaag ccgctcaagc cgatgtgttg aaagaactcg aaggcaaaga
     9361 ggccgtcgtt accgccatcg aaaagaaaaa gcgcagccgc aaccccgccg cgccgttcac
     9421 cacatccacc atgcagcagg atgccgtgcg taaacttggt ttcaccaccg accgcaccat
     9481 gcgtaccgcc cagcagcttt acgaaggtat agacgtaggg cagggcgcca tcggtctgat
     9541 tacctatatg cgtaccgaca gcgtgaattt ggctgacgaa gcgttaaccg aaatccgcca
     9601 ttacattgaa aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac
     9661 caaatccaaa aacgcccaag aagcccatga agcgatccgc ccgacttccg tgtaccgcac
     9721 gcccgaaagc gtcaaaccct tcctgagtgc agaccagttt aaactttatc aaatgatttg
     9781 gcagcgcacc gtcgcctgtc agatgacgcc cgccaaattc gaccaaacca ccgtcgatat
     9841 taccgtcggc aaaggcgtgt tccgagtaac cggacaagtg caaaccttcg caggcttcct
     9901 cagcgtttac gaagaaagca gcgacgatga agagagcgaa gacggcaaaa aactgcccga
     9961 aatgagcgaa ggcgacaaat tgccggtcga caaactctac ggcgaacaac acttcaccac
    10021 tccgccgccg cgctacaacg aagccacact ggttaaagcc cttgaagaat acggcatcgg
    10081 ccgcccctcg acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct
    10141 tgagcaaaaa cgcttcatgc ccaccgacac aggcgacatc gtcaataaat tcctgaccga
    10201 acacttcgcc caatacgtcg attaccactt caccgccaaa ctcgaagacc agcttgacga
    10261 aatcgccgac ggcaaacgcc gctggattcc cgtgatggac aaattctgga aaccgttcat
    10321 caaacaagtg gaagaaaaag agggcatcga acgcgccaaa tttaccacgc aggaacttaa
    10381 cgaaacctgc ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg
    10441 cttcgtcgcc tgtgccggct atcccgaatg cagctacacg cgcaacgtca acgaaaccgc
    10501 cgaagaagcc gccgagcgca tcgccaaagc cgaggctgaa caagccgaac tcgacggacg
    10561 cgaatgcccc aaatgcggcg gtcggttagc gtataaatac agccgtaccg gcagcaaatt
    10621 catcggctgc gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga
    10681 caccggcgtc caatgcccgc aatgcaaaaa aggtaacctc gtcgagcgca aatcccgcta
    10741 cggcaaactg ttttacagtt gcagcaccta tcccgactgc aactacgcca cttggaaccc
    10801 gcccgttgcc gaagaatgcc cgaactgcca ttggccggtc ttgaccatca aaaccactaa
    10861 acgccggggt gtggaaaaag tctgcccgca aaaagaatgc ggctggaaag aacaaatcga
    10921 accgcctgca ccgcaagagt gaagcggttg ggattaagat gaaagaaaat gccgtctgaa
    10981 aggggtttca gacggcattt gtaaattaga aggggctgtc ccagatggct ggggaaattt
    11041 aaattaagtc agaattatcc ctatgagaaa agccgtccaa gccggtacaa acaaaagaaa
    11101 cccatcggac tgtttgccgc aggggtaact gcaagaacgg cagcagggtt ggcagcagtt
    11161 aatcaaaaca cggcagcctg ttattttcta tggcgaaata gtgtttggca catcatcggg
    11221 aaaacttcct ttacacgcat ttggacgaaa tctgcgaaat tatggaggca tacgacatat
    11281 cgtttacgat tacttatccg tcaaaacagt ccgcatttgg aaatgtatga cggcgaagca
    11341 ggggcgggtg aaagttattt ccgtaaaggc agacgcggcc gcagtgccgc cggcaaagcc
    11401 gccgtattcg ggcttttgaa gcgcaacggc aaggtttgta cggttactgt cggacactcg
    11461 aaccgctatt ttattgccta ttatccgcgg acgggtgaaa cccggcagca ttgtttatac
    11521 ggattgttcg tagttacgat gtatcagatt gtaagcggat ttggccgttt ccgtatcaat
    11581 cgcggcacac attttgccga acggcaaaac catatcaacg caattgggaa cttttggaat
    11641 cgggcaaacg tcatttgcgc aagtttgacg gcattcccaa agagcatttc gggctgtatt
    11701 tgaaggagtg cggacggcgt tttgacaaca gtggggtaaa agttcgaatt tccattttaa
    11761 aacaattggt aaagcaggat ttatcccggt tggccgggat tttaaaaaat aaatacattc
    11821 tttaacaaaa caaatcatat cccggcaaaa caaatctgat aaaatacttg gcgtttatta
    11881 acaatctttt taataattta atcttagata tttggagttg atatgcatac ggtcgacaaa
    11941 aa
//
LOCUS       DAWTCI010000063        11645 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWTCI010000063 DAWTCI010000000
VERSION     DAWTCI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11645
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.063"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(91..525)
                     /gene="queD"
                     /locus_tag="WM481_001615"
     CDS             complement(91..525)
                     /gene="queD"
                     /locus_tag="WM481_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFA9080895.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGTIKGG
                     AKDGMVMDFTDLKAIVKQHITDHFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYCRLKNAGLNVCRVKLWETPTSCAEYEGGEGIF"
     gene            complement(613..1128)
                     /locus_tag="WM481_001616"
     CDS             complement(613..1128)
                     /locus_tag="WM481_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFA9080896.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DTDKIHIDGWQVVEWADGYDIAVSETPKTKMPPENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDYVRQHKDNLKDVDNLLALEHIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(1156..1815)
                     /gene="queC"
                     /locus_tag="WM481_001617"
     CDS             complement(1156..1815)
                     /gene="queC"
                     /locus_tag="WM481_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFA9080897.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFRYGQRHA
                     VELERAEWIAQDLGVSQTVLDLSLMRQITHNALMDETAAIETADNGVPNTFVDGRNAL
                     FLLYAAIFAKGQGIRHIIAGVCETDFSGYPDCRGVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLDYIREQTHTCYKGIVGGCRECPSCILRERGLAECLESKKA
                     V"
     gene            complement(2000..2205)
                     /locus_tag="WM481_001618"
                     /pseudo
     CDS             complement(2000..2205)
                     /locus_tag="WM481_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2304..3530
                     /locus_tag="WM481_001619"
     CDS             2304..3530
                     /locus_tag="WM481_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFA9080898.1"
                     /translation="MTFLQRWQGLADNKICAFAWFVIRRFSEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYIDAFRDQANRLTA
                     IGSVMLVVTSLMLIRTIDNAFNRIWRVNTQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDSVLSSGAQQWADALKTAARLAFMTLLLWGLYRFVPNRFVPARQAFVGALITA
                     FCLETARFLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGRTLSVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELSELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(3551..3832)
                     /locus_tag="WM481_001620"
     CDS             complement(3551..3832)
                     /locus_tag="WM481_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9080899.1"
                     /translation="MIREEISAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEAASHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            3911..4027
                     /locus_tag="WM481_001621"
                     /pseudo
     CDS             3911..4027
                     /locus_tag="WM481_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4025..5746)
                     /locus_tag="WM481_001622"
     CDS             complement(4025..5746)
                     /locus_tag="WM481_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HFA9080900.1"
                     /translation="MDYLQNLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDL
                     RALRSKYCLSLKGENLADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLNHFVV
                     LESVSSDGAAVMDPASGRRKVKTEEISRKFTGIALELWPNTRFEAGEEKQEIRILPML
                     RGISGLGRTLFQLLALAAAMEVFALVSPFFMQWVIDHVVVTGDRNLLATLALGFTLLL
                     LVQNTVSVMQGWLGMHFSTTLNVQWKSNVFKRLIDLPTDYFAKRHLGDVVSRFGAVDS
                     IQGTLTSTFFVLVLNSLTAVFTFVLMIVYSPILTGLVAATLVVYIAVRWVAYYPLRRA
                     TEENIIHAAKQSSYFMETVRGIRAVKQFGKGPQRYSAWMGLLVDTVNTGLTVQKLGIW
                     FGLANKLLFGLANILIIYLAAGMVLDGVFTVGVLMAFLAYKGQFEGRIGSLVDQFVQI
                     KMLSLHAERLADTVLNETESEATPDVGIPDISDDIEISVENVSFRYADNEPYVLQNVS
                     FKIGRGESLALIGRSGCGKSTFLDILSGNLPPESGKVMINGHDIYSLPPRFIRNLSAM
                     VRQDDVL"
     gene            complement(5848..6063)
                     /locus_tag="WM481_001623"
     CDS             complement(5848..6063)
                     /locus_tag="WM481_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080901.1"
                     /translation="MDFAHYLKHWKAAVLIYLAISILTDILCYFLNFDGVFYKGRFFS
                     VTVAGPVGALSFLAYLLYLKREENRSH"
     gene            complement(6077..6424)
                     /locus_tag="WM481_001624"
     CDS             complement(6077..6424)
                     /locus_tag="WM481_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080902.1"
                     /translation="MRELTMNEMTAVFGGTEGTWGHRGRVSGEWVGSKVGGALGSFAG
                     AFTGMAGGALVTGGNPAGIAIGGAGGGWVGAEGGSALGGHIGGFAGAKWGKMIDRRNC
                     QCGEDCHCDPCNC"
     gene            complement(6609..7883)
                     /locus_tag="WM481_001625"
     CDS             complement(6609..7883)
                     /locus_tag="WM481_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFA9080903.1"
                     /translation="MNRPKQPFFRPEVAIARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTMEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEEMLRKYRFLSANDAVSKQEMMNVEAELLEQKAKLDAYRREEA
                     GLLQEIRTQNLTLASLPKRHETEQSQLERTIADISQEVLDFEMRSEQIIRAGRSGYIA
                     IPNVEVGRQVDPSKLFLSIVPERTELYAHLYIPSSAAGFIKPKDKVVLRYQAYPYQKF
                     GLASGSVVSVAKTALGRQELSGLGMVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPL
                     QIGMTLEADILHEKRRLYEWVLEPIYSMSGRL"
     gene            complement(7944..8298)
                     /locus_tag="WM481_001626"
                     /pseudo
     CDS             complement(7944..8298)
                     /locus_tag="WM481_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8291..8518)
                     /locus_tag="WM481_001627"
     CDS             complement(8291..8518)
                     /locus_tag="WM481_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080904.1"
                     /translation="MKNWKQFIFFVILVIACYQLLYSLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            complement(8527..9681)
                     /locus_tag="WM481_001628"
     CDS             complement(8527..9681)
                     /locus_tag="WM481_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9080905.1"
                     /translation="MGIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASCKYINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDARDMYAVQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQAKHEEVRKLIYQLIELDFLEIL"
     gene            9933..10214
                     /locus_tag="WM481_001629"
     CDS             9933..10214
                     /locus_tag="WM481_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080906.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            10211..11254
                     /locus_tag="WM481_001630"
     CDS             10211..11254
                     /locus_tag="WM481_001630"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFA9080907.1"
                     /translation="MSKKIKAGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGTAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSDPETVLRDYYRDSPFMDI
                     LPAGSTPETRSVRGANLCRISIRQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     EETHGLGGIPLLP"
BASE COUNT         2766 a         3028 c         2843 g         3008 t
ORIGIN      
        1 aatcgttctc tttgatctga ggcgaggcaa cgccgtaccg gtttaaagtt aatccaccat
       61 accttatttg aattgactat atcgatacgt tcaaaatatt ccctcccccc cttcatactc
      121 cgcgcacgat gtcggcgttt cccacaattt gacgcggcac acgttcagcc ccgcgttttt
      181 cagacggcag tacatttcga ccgccatatt ttcggcagtt gtgcggcagg gcaggcgcag
      241 ggttttcatg ttccagcctt ccaaaagcgc ggcgatttga gattcgcgcc cgttgccgcc
      301 gtggtagatg aaggcgtggt cgaaatggtc ggtaatgtgt tgtttcacaa tggctttcaa
      361 gtcggtaaag tccatcacca taccgtcttt cgctccgcct ttgatggtgc cgtctgaaac
      421 ggtgatttcg agtttgtagg tatgtccgtg caggttttgg catttgccgt catgcccgtc
      481 gagcatatgc gaggagtcga aggtaaatat cttggtaatt ttcatcccga ttccttttca
      541 gtgaaaaccc cgccgctttc cgcccggagg gtggcttgat gtgtcgtttt gcgtggaaac
      601 atatccgctg cttcaaatgg gcaaatagcc ttgaaagccg atttcggcgg gtttgccgtg
      661 cggattcggg gtcaggcgga tatggaaatt gccgatgtgc tcgagcgcaa gcaggttgtc
      721 cacgtctttc aagttgtctt tatgctgccg aacatagtcg gtcaacaggg tttgcagggc
      781 tttttgtttg gcttcggcag gcgtggcggc ggcgaacagc ccgaacgcgt gcgcctcggc
      841 aagctgcccc gcgcgatagc cgccgacatt ggcaaaatac aggcgcggag cgttttcagg
      901 cggcattttc gttttgggcg tttcggatac ggcgatgtcg taaccgtccg cccattcgac
      961 aacctgccaa ccgtcgatgt ggattttgtc cgtatcgccg aaccacgcgg ctttgagtgc
     1021 ggggacggct tcgcggtagt cgtcgcacac ggcaaattgg atgtcgtgca cttcgatatt
     1081 cgaccggccg gcattgccgc cgaggtaaaa catatggagc ttgggcatat cgtattcctt
     1141 gtgctttgcg cgcgttcaga cggccttttt actttccaga cattccgcca gcccgcgttc
     1201 gcgcaagata cagctcgggc attcgcggca gccgccgacg atacccttgt agcaggtgtg
     1261 ggtctgttcg cggatgtagt ccaacacgcc catttcgtcc gccaacgccc acgtttgcgc
     1321 cttggtcagg tacatcagcg gcgtgtggat ttgaaaatcg tagtccatcg ccagattaag
     1381 ggtaacgttc atggatttga cgaacacgcc gcggcagtcg ggatagcccg aaaaatcggt
     1441 ttcgcacacg cccgcgatga tgtgccggat accctgccct ttggcaaaaa tggcggcgta
     1501 aagcaggaaa agcgcgttac gcccgtccac aaaggtattg ggaacgccgt tgtcggcggt
     1561 ttcgatggcg gcggtttcgt ccatcagggc gttgtgcgta atctgccgca tcaggctcaa
     1621 atcgagtacg gtttgactga cacccaaatc ctgcgcgatc cactctgcgc gttccagctc
     1681 gacggcatgg cgttgcccgt atcggaaggt gatggcttgg acgttttcgc gcccgtaggt
     1741 ttggattgcc tgaatcaggc aggtggtcga atcctgaccg cccgagaaga tgaccaaggc
     1801 tttttggttt gacatatcga atcctagttt tggtaacgcg ggaggtttgc gaaccgcgaa
     1861 gttggacagt aaagacgcgg attatacagg caaacgcgtt cagacggcat ctgaacgcca
     1921 tgccgtctga acacgcggcg tatgccgact tgacggcgag ttccgccaag cgtctgcctt
     1981 gtgcgaaggc gatattgttt tcttcggcgg tcaggacggg agccgctgtc gggaatatct
     2041 ttatttgacg gcttcgcaga cggcggacac tttggggacg gtgcgcaata cggcttcgca
     2101 accttcaacg ctgtcgatgc cgcgagtgat tcggcgtgcg ggattgcggg tgctgccgtt
     2161 ttgggaatag tagaggacga ggattttgag gggatttggg ttcatgggcg gattccgtta
     2221 aaatagtttg gtgttcagac ggcataacgg ttgtccagat gccgtctgaa acagtttgaa
     2281 aacatgaaac ctggtaagag attatgacct ttttacaacg ttggcaaggt ttggcggaca
     2341 ataaaatctg tgcatttgca tggttcgtca tccgccgttt cagtgaagag cgcgtaccgc
     2401 aggcagcggc gagcatgacg tttacgacac tgctggcact cgtccccgta ctgaccgtaa
     2461 tggtcgcggt cgcttcgatt ttccccgtgt tcgaccgctg gtcggattcg ttcgtctcct
     2521 tcgtcaacca aaccattgtg ccgcagggcg cggatatggt gttcgactat atcgacgcat
     2581 tccgcgatca ggcaaaccgg ctgaccgcca tcggcagcgt gatgctggtc gtaacctcgc
     2641 tgatgctgat tcggacgata gacaatgcgt tcaaccgcat ctggcgggtt aacacgcaac
     2701 gcccctggat gatgcagttc ctcgtttatt gggcgttgct gactttcggg cctttgtctt
     2761 tgggtgtggg catttccttt atggtcgggt cggttcaaga ctccgtactc tcctccggag
     2821 cgcaacaatg ggcggacgcg ttgaagacgg cggcaaggct ggctttcatg acgcttttgc
     2881 tgtgggggct gtaccgcttc gtgcccaacc gcttcgtgcc cgcccggcag gcgtttgtcg
     2941 gagctttgat tacggcattc tgcctggaga cggcacgttt cctgttcacc tggtatatgg
     3001 gcaatttcga cggctaccgc tcgatttacg gcgcgtttgc cgccgtgccg tttttcctgc
     3061 tgtggttaaa cctgctgtgg acgctggtct tgggcggggc ggtgctgact tcgtcgctgt
     3121 cttattggca gggcgaggcc ttccgcaggg gattcgactc gcgcggacgg tttgacgacg
     3181 tgttgaaaat cctgctgctt ctggatgcgg cgcaaaaaga aggccgaacc ctgtccgttc
     3241 aggagttcag acggcatatc aatatgggtt acgatgaatt gggcgagctt ttggaaaagc
     3301 tggcgcggta cggctatatc tattccggca gacagggctg ggttttgaaa acgggggcgg
     3361 attcgattga gttgagcgaa ctcttcaagc tcttcgtgta ccgcccgttg cctgtggaaa
     3421 gggatcatgt gaaccaagct gtcgatgcgg taatgacgcc gtgtttgcag actttgaaca
     3481 tgacgctggc ggagtttgac gctcaggcga aaaaacagca gcagtcttga gtatttttga
     3541 aactgtattt ttatcctaaa gacattttcc tatctgctga ttccaccgtt tcctttctgt
     3601 gtgccgccat aatttttata atagacaagc cattcaagtt tgcattgact gctttttcat
     3661 tggcaatatc caaatgactg gccgcttcat ctaaaaatag gattttcggt tcgcaatata
     3721 aggcccgcgc caatacgata cgttgttttt gtccgcctga cagtgcgctg cccatatcgc
     3781 cgatcaaggt ttcatagccc ataggcatgg cggagatttc ttcgcgaatc atagcaaggc
     3841 aggcgcaatg ttcgattttt cctctatccg gttctgtatc gaaaaaacag atattctctg
     3901 caattgagcc agcaaatgca aatatagtgg attaaattta aatcaggaca aggcggcgaa
     3961 gccgcagaca gtacagatag tacggcaagg cgaggcaacg ccgtaccggt ttaaatttaa
     4021 tccactataa aacatcgtcc tgcctgacca tcgcactcaa attgcggata aaacgtggcg
     4081 gtaagctgta aatgtcgtgc ccatttatca tgactttgcc tgattcggga ggtagattgc
     4141 cgcttaaaat atccaaaaat gtcgatttac cgcagcccga tcgtccgatt aacgcaagcg
     4201 attcaccacg tccgatcttg aagctgacgt tttgtaaaac atatggctcg ttatcggcat
     4261 aacggaaaga aacattttcc actgagattt cgatgtcgtc tgaaatatca ggaatgccta
     4321 catccggcgt agcttccgat tctgtttcat tcaatacggt gtcagctaac cgttccgcat
     4381 gaagtgacag cattttaatt tgcacaaact gatcgaccag cgagccaata cgtccttcaa
     4441 actgtccctt ataagccaaa aaagccatta gtacgccgac ggtaaagacg ccgtccaata
     4501 ccatgcccgc agctaagtaa ataatcaaaa tatttgccaa accgaaaagc agcttgtttg
     4561 ccaagccgaa ccaaattccc agtttctgca ccgtcagtcc cgtattaacc gtatccacca
     4621 gcagccccat ccatgcagaa tatcgttgag gacctttacc aaattgcttg actgctcgga
     4681 taccgcgtac cgtttccata aaatagctgc tttgtttggc agcatggatg atgttttcct
     4741 ccgtcgcccg tcgcaacgga taataggcta cccaacggac tgcgatataa actaccagtg
     4801 tcgctgcaac caaacctgtc agtatcgggc tgtacactat cattagcaca aaagtaaaaa
     4861 cagccgttag gctgtttaaa accaacacga aaaacgttga agtcagagtt ccctgaatac
     4921 tatccaccgc cccgaagcgc gataccacat cgcccaaatg ccgtttagcg aaataatctg
     4981 taggcaaatc tatcaaacgt ttgaaaacgt ttgatttcca ctgaacattt aacgtcgttg
     5041 aaaaatgcat gcccaaccag ccctgcatga cagacaccgt attttgtacc agaagtagca
     5101 aggtaaagcc caaagccagc gtcgctaaca gattacgatc gcccgttacc actacatggt
     5161 cgatgaccca ttgcataaaa aacggcgaca ccaaagcaaa cacttccatt gctgctgcca
     5221 aagccaaaag ctgaaacaat gtccgcccca gcccagaaat cccgcgcaac atgggtagga
     5281 tgcggatttc ctgcttttct tcccctgcct cgaaacgcgt gtttggccac agttccaaag
     5341 caattcccgt aaacttgcgc gatatttcct ccgtcttgac tttgcgtcgt cccgaagccg
     5401 gatccatgac ggcagccccg tccgaagata ccgattccag caccacaaaa tgattcaaat
     5461 cccaatgtag aatacagggc aggcgcaaac tgcccaattc gtctaaatcc agcctcaacg
     5521 cccgtcccgt cagccccata tcatcagcaa aacgaacaat atctgccaaa ttctcaccct
     5581 taagtgacag acagtatttt gaacgcagtg cgcgcaaatc cgtatagaaa ccataaaatc
     5641 cggccacagc cgctagacat gccaagccac actccgctac ttctgtttgc agtataacgg
     5701 gcagcttttt tgtcaagccc aaagacaggt tttgcagata atccatagat ttccttgttg
     5761 aatcagaaaa gagagatggg aagacgggaa tggtttggaa aggccgtctg aaaaggtttc
     5821 agacggcctg cagccccata ttgactttca atgcgaccgg ttttcctcgc gtttcaaata
     5881 cagcagatac gcaagaaaag acaaagcccc tacaggtcct gcgacggtaa ccgaaaaaaa
     5941 cctgcctttg taaaacacac cgtcgaagtt taaaaaatag caaaggatgt cggtcaggat
     6001 gctgatggca agataaatca gcacagcggc tttccagtgt ttcaggtaat gtgcgaaatc
     6061 catttcaaac tcccgatcaa cagttgcacg ggtcgcaatg gcagtcttct ccgcattggc
     6121 agttgcgcct gtcgatcatt tttccccatt ttgcaccggc aaaaccgccg atatgcccac
     6181 ccaatgcaga accgccctca gctcctaccc atccgccgcc tgcgccgccg atggcaattc
     6241 cggcaggatt tccgcctgtt accaatgcgc cgcccgccat tcccgtgaat gctccggcaa
     6301 acgaccctaa agcgccgccg accttgcttc cgacccattc tccggagact ctgcccctgt
     6361 gcccccaagt accttctgtg ccgccgaaaa cagcggtcat ttcgttcatg gtcaattctc
     6421 tcatttcaaa actcctttta tcgtcaatcg aaaaattgaa agggcagata ccctatcctt
     6481 gcttttacag acagagtatc tgtaaaaggg ggtagtttca ggcagcttca gtgtttatgt
     6541 ttttatgtag tgttttacag gtcgtctgaa gaggtttcag acgacttttt atctttcagc
     6601 ctcaagcctt acaacctgcc cgacatacta taaatcggct ccaataccca ttcgtacagc
     6661 cgccgtttct cgtgtaggat gtctgcttcc aacgtcatgc cgatttgcag cggtttttcc
     6721 tcaccgtatg cagtgatggt tggtttgtcg ggttttattt tcacgagata aacaggttcg
     6781 ttgctcttcg ccaaatcgga ggataccatg cccaatcccg acaattcctg tctgcccagt
     6841 gccgtttttg ccactgatac gacactgccg gaagcaagcc cgaatttttg atagggatat
     6901 gcctgataac gcaggacaac cttgtctttc ggcttgataa agcctgctgc actgctgggg
     6961 atatatagat gggcatataa ctcggtacgt tcgggaacaa tgctcaagaa cagtttggaa
     7021 ggatcaacct gccgtccgac ttcgacgttc ggtattgcta tataacccga ccgtcctgca
     7081 cggatgattt gttcagagcg catttcaaaa tccaaaactt cttgagaaat atcggcaatg
     7141 gtgcgttcaa gctggctttg ttctgtctca tgccgtttgg ggaggctggc caatgtcaga
     7201 ttctgcgtgc ggatttcctg aagcagcccg gcttcttctc ggcggtaggc atcaagtttg
     7261 gctttctgct ctaaaagctc tgcctcgaca ttcatcattt cttgttttga cactgcatca
     7321 ttggcggata ggaaacgata tttccgcaac atttcttccg caagtctaat gcgccttttc
     7381 tggccgtcta tctgttgcga aatatggagt ttctggtttt ccaaacgttc gacagttgct
     7441 ttaaggctgc gcgtttcatt ctcgtgtatc agcttcagac gacccagttc ctgttctgcc
     7501 aacgttttct tcaaaactgc ctccgttttc aactgctgct gcacgctacc tcctgcgccg
     7561 aaacgtgagg tcgaaagcgc aaatagcttg tcgccagcct taaccttttc tccatcttcc
     7621 acgaatttcg ctgtaattgt ccccgtatcc ggtgcataca ccctgattac gcccgatgca
     7681 ggtagaattt gtccctccat tgttgtcttt cgcgtatagt taccgaatat caaaaacagg
     7741 ataatcagta acgcagatat cgatgcaaat gtcgtccaca gggaaaatga caacgggcgt
     7801 gtcagaatca ctttacccgt caggctggtt tggcgggcaa tggcgacttc agggcggaag
     7861 aaaggttgct tgggtctatt cataaaattg aagttaagaa agtttcagac gacccctaga
     7921 gatcgtctga acaatgagaa atatcagcag taatctgtac cgtcagtgta gccgtttcct
     7981 gatttatctg cttttgttgc gggagcagtt aatccatgtt caatctcaaa gattggtctt
     8041 ccgttataag gaggtgcatt aacggcatca tttacccaat cacgagtcag attgtataca
     8101 ccatttgcac catatagcac cgaaaaaatt tttcggcagg aaatgaactg tcgctgcagc
     8161 agcaggtatt gcaacaaaat ccccccatgt gggacctcct ttggttgtgg cagcattagc
     8221 tacatttcca gctatattgt ctgttacagg acctccccct gaaaccagct tcaattcatg
     8281 aagttgaagt tcaatcattt ttatactcct tttcttggtt ggtattccta aaaattcggc
     8341 taacaaaaac atatggcaga tatattgaaa aaaaattcaa agtaccctga ataaaattca
     8401 aattccaact atatttgtta atgtagtcga gaagaaacat atctgataaa gaatatagca
     8461 attgataaca agctattact aatattacga aaaatataaa ctgcttccag tttttcatag
     8521 aatccctcac aaaatttcca gaaaatctaa ctctatcaac tgatagatca acttcctaac
     8581 ttcttcatgt tttgcctgat tgaagttaac cagtagattt ttcaacaata acggttcatt
     8641 cttaccgatg tgttctaaca cttttttccc caactcatct acgcttatct tcatcccatt
     8701 cccaatcaaa tatccctttt ccaacgtatc caaattattg gcatttagtc tcaacctgac
     8761 gtcgtctgaa agcggagtag cgttgggatt cgcgaactgt tcgagatgaa aagcggtatc
     8821 ggtacgttct ttgccgagaa agtcttcact gaaagcttcg taattgacgg ggtcggcaat
     8881 catggcggca atttgtgcgg cagtatcgtt gatacgcgtc ctatcttgct cccagtctga
     8941 gaaactgtgg cgcagacttt ctatggtggg aaatttcttc attagccact cgaggtaatt
     9001 atagccgttt ggtgggaagg taccgacagc gaagtggaag gtttcacagc cgagcgggat
     9061 aggtctgtgc caccaaccgc gtgggatgta gaggacatca cctgcttcaa ggataatatc
     9121 catatcgata tgttcaggaa tggaaatatc agtatcttta gtctgttgca tatacaatgg
     9181 catagggaaa tcaggggcag taagttgcca acgtttcttg ccgaaaagct ggacggcata
     9241 catatctcgg gcgtcccaat ggtttttata agattcgtcg ctgccaaaag caagatatcc
     9301 actaacaata gtatgtgcgc cggcaaagcg ggcgacttga cgggcgatat ggtctgaaaa
     9361 cggctcgttg ttaatatggt tatagactaa cgacgcaccg ttcttcatat gttcgtagat
     9421 aacggattta ataaaacggt agcgagtttt gcccaaatcg tcgaaacttt cgacgtattc
     9481 ttctttagga acgattgcgc cttttttacg cagatgaaac agcggtgcgg ttgggtctgc
     9541 tcgttggtat atctcgttga tatatttaca agatgcggct tcgagattcc gaaccgctcc
     9601 tttaaagagc ttgggctttt gatacagata agtctgtcgg aacgttttag gactaatgcc
     9661 gaagtcgaga tggatgccca ttacttcccc ttactcagaa aatatttaaa atttataatg
     9721 ttacatatag ttacaaatat tagagttttt tgtgtgtgcg tcaaggaatt gttgacaatt
     9781 ttagttaaaa atttgtctcc aaacggaaaa aagcggtttg ttttttgttg ttaacatttt
     9841 tattgtaatt aaataaaagg tcgtctgaaa acggttttca gacgaccttt tgctataatc
     9901 gggcttcatc gccccgttcg gtttggaacc ttatgaaaac cctcgtcctc ctcctgcttt
     9961 ccttctccac gaccaccgct ttcgccgcat acggtttggg tttggggcag gcaccgaaat
    10021 atcctgccgg ctttcgcgcc tacggttatg tttattccgg acggcagggc tgggttttaa
    10081 aaacagaggc ggatgccatt aaattagaca cgcttttcaa acgctttgtg taccgtcctt
    10141 ccgccgccaa tcaaaacccc gtcggacagc gttcggacgg catacccgcc aaccacacaa
    10201 aggaaaaacc atgagcaaaa aaatcaaagc cggcattgtc ggcgcgacgg gctacacggg
    10261 cgtggaactg ctgcgcctgc ttgccgccca tccagatgtc gaagtcgccg ccgtaaccag
    10321 ccgcagcgaa gcgggaaccg cagttgccga ttactttcca agtctgcgcg gcgtgtacgg
    10381 cctcgccttc caaacgcccg acgaggcagg tttggaacaa tgcgacatcg tcttcttcgc
    10441 cacgcccaac ggcatcgcca tgaaagacgc gccgcgcctg attgaacagg gcgtgcgcgt
    10501 catcgacctt tccgccgact tccgcatacg ggacattccg acctgggaac actggtacgg
    10561 catgacccac gccgcccccg gcctcgtttc ccaagccgtg tacggattga gcgaactcaa
    10621 ccgcgaagcc gtcgcacagg cgcgcctcgt cgccaacccc ggctgctacc cgacctgcgt
    10681 atccctgccg ctcgtgccgc tgttgcggca atgccgtctg aagcccggta tgccgctgat
    10741 tgccgactgc aaatccggcg tgtccggcgc gggcaggaaa ggcaatgtcg gttcgctgtt
    10801 gtgcgaagcc ggcgacaact tcaaagccta cggcacagcc ggacaccgcc acctgcccga
    10861 aatcaggcag accatcgccg ggcttcagga cggcatcgcc gaaggattcg tgttcacgcc
    10921 gcacctcgcg ccgatgatac gcggtatgca cgccaccgtt tacctccacc tttcagacgg
    10981 cagcgacccc gaaaccgtcc tgcgcgacta ctaccgcgac agcccgttca tggacatcct
    11041 gcccgccggt tcgacacccg aaacccgcag cgtgcgcggc gcaaacctct gccgcatcag
    11101 catccgtcag gcggcgcaat ccgatgtgtg ggtcgtcctt tccgtcatcg acaacctcgt
    11161 caaaggcgcg gcgggacagg cggtgcaaaa tatgaacatt atgttcggac tggaggaaac
    11221 acacggcttg ggcggcatcc ccctgctccc ctgaagcgca aacggcaaac cgcaggcatc
    11281 gtgcctgcgg ttttttgatg ccgtctgaaa gcggcggttt ttttgggttc ggacggcttt
    11341 tggctcatca ttccgcgaaa acaaaaatct aaaataccgt cattcccgca aaagcgggaa
    11401 tctagtttat ccggcttcag caatttccga cacatttcca cacgcttcga ttccgtcatt
    11461 tctccggttt caatcgtttc cgataacacc gtggtttttc atttctagat tcccgcctgc
    11521 gcgggaatga cggcgggggg cttgccgttt tcccgataag cctctgcggc ttctcattgc
    11581 cggatttccg cccgcttggg aataacggga tttaaaatca tgaaaatgtg tcaaaaataa
    11641 tatag
//
LOCUS       DAWTCI010000064        11331 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWTCI010000064 DAWTCI010000000
VERSION     DAWTCI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.064"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(64..1143)
                     /gene="apbC"
                     /locus_tag="WM481_001631"
     CDS             complement(64..1143)
                     /gene="apbC"
                     /locus_tag="WM481_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFA9080908.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVGQRSDGIHIALHF
                     GFPVAHIAAALADAVQETLMPETGGAHIHLSIDTEIGTHKVRPGVATIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPARLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1362..1437
                     /locus_tag="WM481_001632"
     tRNA            1362..1437
                     /locus_tag="WM481_001632"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1395..1397,aa:Lys,seq:ttt)
     gene            1510..2019
                     /locus_tag="WM481_001633"
     CDS             1510..2019
                     /locus_tag="WM481_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFA9080909.1"
                     /translation="MKKKLLSGIKFAVRTALVFLLVSLFLDWVRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVSDEDGGLARSWRIAATPAVVLVKNGKMVRYTTGISSYWGLRA
                     RIFQADFFG"
     gene            2360..2800
                     /gene="nadR"
                     /locus_tag="WM481_001634"
     CDS             2360..2800
                     /gene="nadR"
                     /locus_tag="WM481_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFA9080910.1"
                     /translation="MPTQSKHASINIGLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVYLKLTSEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            2915..3415
                     /gene="hpaC"
                     /locus_tag="WM481_001635"
     CDS             2915..3415
                     /gene="hpaC"
                     /locus_tag="WM481_001635"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFA9080911.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRPLD"
     gene            3431..4126
                     /locus_tag="WM481_001636"
     CDS             3431..4126
                     /locus_tag="WM481_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HFA9080912.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIENALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVANGDVLTDIDFTAAFQTASSLPGHISAHLWLVGNPPHNPDGDFSLLPDGSVRPEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLLNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAAAWK"
     gene            complement(4180..4590)
                     /locus_tag="WM481_001637"
     CDS             complement(4180..4590)
                     /locus_tag="WM481_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFA9080913.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFAAFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNRTFLVLSGLATGASWLAYFKALQLGNAPQVAPVD
                     KFSLVLVALMAVIFLDERPNTQEWIDLGLVTAAC"
     gene            4765..6441
                     /locus_tag="WM481_001638"
     CDS             4765..6441
                     /locus_tag="WM481_001638"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFA9080914.1"
                     /translation="MSFKTDAETAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RFGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIDNQPNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6555..7646
                     /gene="ychF"
                     /locus_tag="WM481_001639"
     CDS             6555..7646
                     /gene="ychF"
                     /locus_tag="WM481_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFA9080915.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNRFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERALLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVIAYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            7684..7923
                     /locus_tag="WM481_001640"
     CDS             7684..7923
                     /locus_tag="WM481_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080916.1"
                     /translation="MPSEAGFGGFRRSHTAGMQPHQNKIPPAFPICPPRFLQNKPPAL
                     PLREAVRYSEYPEPRYKMTFQTAFAQPAAFQVKTL"
     gene            7920..9795
                     /locus_tag="WM481_001641"
                     /pseudo
     CDS             7920..9795
                     /locus_tag="WM481_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            9857..11152
                     /gene="tyrS"
                     /locus_tag="WM481_001642"
     CDS             9857..11152
                     /gene="tyrS"
                     /locus_tag="WM481_001642"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFA9080917.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVGSIR
                     SQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIGVVEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSRLTES
                     DFEQLALDGLPAFEVSDGINAVEALVKTGLAASNKEARGFVNAKAVLLNGKPAEANNP
                     NHAAERPDDAYLLIGEYKRFGKYTILRRGKRNHALLVWK"
BASE COUNT         2510 a         3104 c         3161 g         2556 t
ORIGIN      
        1 atgaagcccg tcggcaggcg cggggcttgg gacggcattg ctgttgcggt ttcgggcgcg
       61 gctttattcg acgacaattt tagggaaccg gctgctgaag tctttgcctt tgtcggcaat
      121 gcccagggcg atttggaatg cggcatcggt gtagattcgg gcgatggcgg ggtgttcgtc
      181 gaacagtcgc gccggtgtgc cgccgtccat ggcttcgcgc acgggcaggc ttaggggaag
      241 ctgtccgagc agggggacgt tgaggcgggc cgccaaatct ttgccgccgt ccgtgccgaa
      301 cagtgcttcg ctatgcccgc agttggagca gatgtggacg gacatgtttt ccaatacgcc
      361 taaaatggga atgttgacct tgcggaacat atccacagcc ttgcgcgcgt ctatcagcgc
      421 gatgtcctgc ggcgtggtta cgatgacgga cccggttacg gggatgcgtt gggacagcgt
      481 gagttggatg tcgcccgtgc cggggggcag gtcgatgaaa agatagtcca cttcgtccca
      541 ctcgctttgg aacatcagct gctgcaaggc ttggctgacc atcggcccgc gccagacgac
      601 ggcttggccg gtatcgacta gaaagccgat ggacataacc tgtatgccgt ctgaagattc
      661 gacgggaatc agtttttggt ttttctgatc gggtttgcgg tcgtgtacgc ccagcatcgt
      721 gggctggctc ggaccgtaaa ggtcggcatc gagcacgccg acgcgtgcgc ccatgcgcgc
      781 cattgcggcg gcaaggttgg cagtggtggt tgatttgccc acgccgcctt ttcccgatgc
      841 gacggcaatg atgtttttca cgcctttgat ggtggcgacg ccgggccgga ctttgtgtgt
      901 gccgatttcg gtgtctatgg acagatggat gtgcgcgccg cctgtttcgg gcatcagggt
      961 ttcttgtacg gcatcggcga gggcggcggc gatgtgcgcg acagggaagc cgaaatgcag
     1021 ggcgatgtgg atgccgtctg aacgctgtcc gaccgaacgg acggcctttt cgccgccgag
     1081 cgtgcgtgcc gtattcggaa cggcgacggt gtcgaggagg gtgcggatgt tttgtatatt
     1141 catgatgtgt gcctctgttc gcaagtcgtg tgaggggaag atagacggaa agtgtacatg
     1201 attttgggca gtttgctttt ttcttgtgcg aacttttaca cggtatgcgg cggattgtga
     1261 aaatccgttg ccaaaatatc ccgtgcaaac agcagtctgc aattaagcct gtaaaatttg
     1321 cttgacggcg gggaacgggg ctgtataatg cacggcttat cgggtcgtta gctcagccgg
     1381 tagagcagcg gacttttaat ccgttggtcg agcgttcgaa tcgctcacga cccaccagat
     1441 aaccggaagc caagtttcga gacttggctt ttttgtttgc ctgccggggc gtaccgtttg
     1501 cgggggtgta tgaaaaagaa actgctttcg ggcatcaaat ttgccgttcg gacggcattg
     1561 gtgtttttgc tggtgtcgct gtttttggat tgggtacgca agcccgacga acctgccggg
     1621 gcggcagggc ggcctttgac cctgctgtcg gggcagcggc tgactttggg acagtttagc
     1681 cgggatagga cggtgctggt gtatttttgg ggaagctggt gcggcgtgtg ccgttatcag
     1741 tcgccgataa tcgatgattt ggcggcggac ggcgtgccgg tcgtcggcgt ggcggtacgt
     1801 tccggcagcg cgtccgaagt ggcggcatat atggcaaaac ggggcttggg ctttccgact
     1861 gtcagcgatg aggacggggg tttggcaagg tcttggcgga ttgccgcaac gcctgccgtc
     1921 gttttggtca aaaatgggaa aatggtccgc tatacgacgg gaatcagcag ttattggggc
     1981 ttgcgcgcac gaatttttca ggcggatttt ttcggttaac ttttgttttt gtcaaacttt
     2041 ccgctatgta gagaatcaaa cggcatatgc cttgcgcggc ggatgcggcg gcttttgttt
     2101 tttgggaaag agccggcgcg cctgccgatg taatcccttg ttgtgattgt gggaaaaata
     2161 gattaaaata taactattaa aatattttca gataggatta tcggaattaa agtcttttat
     2221 acccggtcgt ccgatgaggt ttatagcgta ttgttgctat atgttcgttt tgttatataa
     2281 cggttgcatc aaaaattacg cccacaggct ttcccgacgg tttgaaagtt tgattttcga
     2341 taacttggag acttaaacaa tgcctaccca atcaaaacat gcgtctatca atatcggtct
     2401 gatacaggca agggaagccc tgatgaccca attcaggcct attctgaatc aggcgaatat
     2461 taccgatcag caatggcgga ttatccgtct tttggcggaa aacggcacat tggattttca
     2521 ggatttggcg aatcaggcgt gcattttgcg ccccagcctg accggtatcc tgacccgcct
     2581 tgaaaaagcg gggttggtcg tccgcctgaa accttccaac gaccaacggc gcgtttatct
     2641 gaagctgact tccgagggcg agaagctgta tgaggaaatc ggcgaagaag tggacgagcg
     2701 ttacgacgct atcgaggaag tgctgggccg cgagaaaatg ctgctgctta aagacctgtt
     2761 ggcagaactt gccaaaatcg aggatgcgtt gaactcgtaa tacgccgtaa tgcgcggaaa
     2821 cgtccgaccg acggcttttt gaatcagaac tgctgcacat gggggatgcc ttgtgtgcag
     2881 cattcttata taggggacgg tttaaagggg aaaaatggcg gatttgcaga aaacttttca
     2941 aacttcgttc cgtgatgcga tggcatcctg tgcggcaggc gttcatgtca tcacgacaga
     3001 cggtgcggca gggcgttacg gcattacaat gacggcggtt gcgccggtta ccgacgagcc
     3061 gccgaccgtg atgctgtgca taaaccggag tgcgcgaatc attccgatcc tgtcggaaaa
     3121 cggcagcctc tgcatcaata tgctggcgga cgaacatcag gatgttgccg aacattttgc
     3181 cgggctgacc ggcctgtcgc ccgaagagcg gtttgcctac catatttggc atcgcggcaa
     3241 aacgggacaa cttgaaatag aaggcgcgtt ggcgcacctg cacgggcata ttgtcggcaa
     3301 acatgaaatc ggcacgcatt ttgtgtttta cgtcaggctc gacgaaatca aaaactgcgg
     3361 gtgcaaacgc cccgcgctgc tgtatttcag acggcagttt aggcctttag actgatattc
     3421 ggacagatat atgaaagcga tgatactggc ggcaggacgc ggcgagcgta tgcgcccttt
     3481 gaccgacacc actccgaagc cgctgctcga tgtggcgggt aagcctctaa tcggttggca
     3541 cttgtgccgt ctgaagcagg cggggtttac cgaaatcgtc atcaaccacg cttggctggg
     3601 tcggcagata gaaaatgctt tgggcgacgg ctcggcttat ggcgtgaaca tcgcctattc
     3661 gcccgaaccc gcaggcggtt tggaaacggc aggcggcatc gcgcaggcat tgccgctgtt
     3721 gggtgggcag ccgtttttgg tggccaacgg cgacgtgctg accgacatcg attttaccgc
     3781 cgcgtttcag acggcatcgt ccctgcccgg gcacatttcc gcacatttgt ggctggtggg
     3841 aaatcccccg cacaaccccg acggcgattt ttccctgctg cccgacggca gcgtgcggcc
     3901 ggaagtatcc ggcggcaacg gactgacatt cagcggcgtg ggtatttacc gtcctgaaat
     3961 gtttgacgga atcgaagcgg gcagtgtggc aaaactcgcg cccgtattgc tgaacgaaat
     4021 gcggcaaaac cgcgtgagcg gtcagaagca tacgggcctg tggctggatg tcggcacggt
     4081 atgccgtctg aaggaagcgc aagcccttgc agcggcttgg aagtgaaaac tcggtttcag
     4141 gcggtatggc ggattcggtt taacgtttca gtgccaacgt caacacgccg ccgtaaccag
     4201 ccccaagtct atccattcct gcgtgttcgg gcgttcgtct aagaaaatca ccgccatcag
     4261 cgcgaccaag accaggctga atttgtcgac gggcgcgact tgcggggcgt tgcccagttg
     4321 cagggctttg aagtaggcga gccaagatgc gccggtggcg agtccggaca aaaccagaaa
     4381 cgtccggttg cgccccgtga agccgtttac gccctgccat ttgccggtgt aggtcaaaaa
     4441 caataccaag gcggcgagga tgaccaaggt gcggataaag gcggcgaaat ccgaatctat
     4501 gccctgcaag cccattttgg cgaaaacggc ggtcaatgag gcgaagcctg ccgatgccaa
     4561 tgcccaaaac agccatgcgt tgctgcccat gttttctcct ttgattgtga acaatatgaa
     4621 cggtattttt gttgctgcgt caaaaatttc actgcgggtt tggtgcggat aacgttataa
     4681 tatgcctgat attattttca atccacctgt ttgtcgcctg atgctttcag acggcatgtc
     4741 cctcctcatt tctaaaggaa aatcatgagc ttcaaaaccg atgccgaaac cgcccaatcc
     4801 tccaccatgc gcccgattgg cgaaattgcc gccaagctgg gtttgaacgt tgacaacatt
     4861 gagccttacg gtcattacaa agccaaaatc aatcctgccg aagcgttcaa gctgccgcaa
     4921 aaacaaggca ggctgatttt ggttaccgcc atcaacccga ctccggcggg cgaaggcaaa
     4981 accaccgtaa ccatcggttt ggcggacgca ttgcgccata tcggcaaaga ctctgtgatt
     5041 gctttgcgcg agccttcttt gggtccggtg ttcggcgtga aaggcggcgc ggcaggcggc
     5101 ggctacgcgc aagttttgcc gatggaagac atcaacctgc acttcaccgg cgacttccac
     5161 gccatcggtg cggcgaataa cctcctcgcc gccatgctcg acaaccatat ctaccaaggt
     5221 aacgagttga acatcgaccc caaacgcgtg ctgtggcggc gcgtggtcga tatgaacgac
     5281 cgccagttgc gcaacatcat cgacggtatg ggcaagcctg ttgacggcgt gatgcgtccc
     5341 gacggcttcg acatcaccgt cgcctccgaa gtgatggcgg tattctgcct tgccaaagac
     5401 atcagcgatt tgaaagagcg ttttggcaat attctcgtcg cctacgccaa agacggcagc
     5461 cccgtttacg ccaaagattt gaaggcacac ggcgcgatgg cggcattgct aaaagatgcg
     5521 attaagccca atttggtgca aaccatcgaa ggcactccgg cctttgtaca cggcggcccg
     5581 ttcgccaaca tcgcccacgg ctgcaactcc gttaccgcaa cccgtctggc gaaacacctt
     5641 gccgattacg ccgtaaccga agcaggcttc ggcgcggact tgggtgcgga aaaattctgc
     5701 gacatcaaat gccgccttgc cggtttgaaa cctgatgcgg cagtcgtcgt ggcgactgtc
     5761 cgcgccctga aatacaacgg cggcgtggaa cgcgccaacc ttggtgaaga aaacctcgaa
     5821 gccttggcaa aaggtttgcc caacctgttg aaacacattt ccaacctgaa aaacgtattc
     5881 ggactgcccg tcgtcgttgc gctcaaccgc ttcgtgtccg actccgatgc cgagttggcg
     5941 atgattgaaa aagcctgtgc cgaacacggc gttgaagttt ccctgaccga agtgtggggc
     6001 aaaggcggcg cgggcggcgc ggatttggcg cgcaaagtcg tcaatgccat cgacaaccaa
     6061 cctaataact tcggtttcgc ctacgatgtc gagttgggca tcaaagacaa aatccgtgcg
     6121 attgcccaaa aagtgtacgg cgcggaagat gtcgatttca gcgcggaagc gtctgccgaa
     6181 atcgcctcgc tggaaaaact gggcttggac aaaatgccga tctgcatggc gaaaacccaa
     6241 tattcattga gcgacaacgc caaactcttg ggctgccccg aaggcttccg catcaccgta
     6301 cgcggtatca ctgtttccgc cggcgcgggc ttcatcgttg cgttgtgcgg caatatgatg
     6361 aaaatgccgg gcctgccgaa agttccggct gccgagaaaa tcgatgtgga cgaacacggc
     6421 gtgattcacg gcttgttctg aacggttttt gaaaccggat gccgtctgaa gccgtttcag
     6481 acggcatttt ttcggaacgc gggcggcggt atgctataat ccgccgttaa atttctctat
     6541 tttcaggaaa aaccatgagt ttgaaatgcg gcatcgtcgg tttgcccaac gtcggcaaat
     6601 ccaccctttt taacgcgctg acccaatcgg gcatcgaagc ggcaaactat cccttctgca
     6661 ccatcgaacc caacgtcggc atcgtcgaag tgcccgaccc gcgtatggcg gagctggcga
     6721 aaatcgtcaa tccgcaaaaa atgcagcccg ccatcgtcga gtttgtcgat attgccggct
     6781 tggttgcggg cgcgagcaaa ggcgaaggct tgggcaaccg gttccttgcc aacatccgtg
     6841 aaaccgatgc catcgtcaac gtcgtgcgct gctttgacga cgacaacatc gtccacgttt
     6901 ccggcaaagt cgatccgatt gccgacatcg aaaccatcgg caccgaattg gcgcttgccg
     6961 acttggcaag tgtggaaaaa gccatcgtcc gcgaagaaaa acgcgcccga tcaggcgaca
     7021 aagacgcgca aaaactagtc gatttgtgca aaaaactgct gccgcatctg gacgaaggca
     7081 aacccgtgcg ttccttcggt ttggacgcgg aagaacgcgc gctgctgaag ccgctgttcc
     7141 tgctgaccgc caaacctgcg atgtatgtgg gaaacgttgc cgaagacggt tttgaaaaca
     7201 acccgcacct cgaccgcctg aaagaattgg cggcaaaaga aaacgccccc gtcgttgccg
     7261 tctgcgccgc gatggagagc gaaatcgccg aattggaaga cggtgaaaaa gccgaatttc
     7321 tcgccgaaat gggcttggaa gaaccgggct tgaaccgcct catccgcgcg ggttacgacc
     7381 ttttgggact gcaaacctac ttcaccgccg gcgtgaaaga agtccgcgcg tggacgatac
     7441 acaaaggcga caccgcgccg caagccgccg gcgtgatcca tacggatttt gaacgcggct
     7501 tcatccgcgc ccaagtgatt gcctacgatg attttgtctc gctcggcggc gaagccaaag
     7561 ccaaagaagc cggcaaaatg cgcgtggaag gcaaggaata cgtcgtgcag gacggcgacg
     7621 tgatgcactt tttgtttaac gtgtaaccca aatgcggcag gttttcagac ggctttgccg
     7681 gaaatgccgt ctgaagccgg ttttggtggt tttcgacgtt cccataccgc cggaatgcag
     7741 ccgcatcaaa ataaaatccc gcccgcattt ccgatttgcc ctccccgatt cctgcaaaac
     7801 aaaccgcctg ccctgccgtt acgggaagcc gtccggtatt ccgaatatcc cgaaccccga
     7861 tacaaaatga cctttcagac ggcatttgca cagcccgccg cgtttcaagt aaaaacatta
     7921 tgagccaagc cttaccctac cgcccggaca tcgacacatt gcgcgccgcc gccgtcttgt
     7981 ccgtcatcgt gttccatatc gaaaaggatt ggctgccggg cgggtttctc ggtgtcgata
     8041 tattctttgt gatttcaggc tttttgatga cggcgatcct ccttcgcgaa atgtccgggg
     8101 ggcgtttctt cctcaagaca ttttatatcc gccgcatcaa acggattttg cccgcatttt
     8161 tcgccgtatt ggcggcaacg ctggcaggcg gcttcttttt attcaccaaa gatgatttct
     8221 ttcttttgtg gaaatccgcg ctgaccgcct tgggtttcgc ctccaacctg tattttgcaa
     8281 gggggaagga ttatttcgat cccgcgcagg aagaaaagcc cctgctgcac atctggtctt
     8341 tgtcggtcga agaacaattt tactttgtct ttccgatatt gctgttgctt gtcgcccgca
     8401 aaagcctgcg cgtacagttc ggcttcctcg ccgcattgtg cgccttaagc cttgccgctt
     8461 cctttatgcc ttccgcgctc gataaatatt acctgcccca cctgcgcgcc tgcgaaatgc
     8521 tggtcggatc gctgaccgcc gtgcggatgc ggtaccggca acagcggaat cccgccgtcg
     8581 ggaaacggta tgccgccgtc ggcgcattgt tttccgcgtg catactgtcc gcctgcctgt
     8641 ttgcctattc ggaacaaacc gcctatttcc cgggctccgc cgccctgatt ccctgtctgg
     8701 ctgttgccgc gctgatttat ttcaaccatt acgaacaccc gcttaaaaaa ttttttccaa
     8761 tggaaaatca ccgttgccgc cggtttgatt tcctattcgc tttatctgtg gcattggccg
     8821 atattggcct ttatgcgcta tatcggcccg gacaacctgc cgccttattc gccggcggca
     8881 gcgatcgtcc tgaccctggc gttttccctg atttcttatc actgcatcga aaagccgttt
     8941 aaaaaatgga aaggctcgtt cgcacaatcc gttttatgga tttatgcctt gcctatgctc
     9001 gttttggggg cgggctcgtt tttcgcgatg aggctgccgt ttatggcgca atacgaccgc
     9061 ttggggctga cgcgttccaa cacctcctgc cacaacaata ccggcaaaca atgcctgtgg
     9121 ggggatacgg aaaaacagcc ggaactgctg gttttgggcg actcccacgc cgaccattac
     9181 aaaacattct tcgatgccgt gggcaaaaaa gaaaaatggt ccgccactat ggtttccgcc
     9241 gacgcctgcg cctatgtgga aggctacgcg tcccgtgtgt tccaaaactg ggccgcctgc
     9301 cgcgccgttt accgctatgc cgaagaacac ctgccccggt atccgaaagt ggttttggcg
     9361 atgcgctggg gcagccagat gcccgaaaac agccgctccc ttgcctacga tgccggtttt
     9421 ttccaaaaat tcgaccgtat gctgcacaaa ctctcatccg aaaaacaagc cgtttacctg
     9481 atggcggaca acttggcttc gtcttacaac gtccagcgcg cctatatctt gtcttcacgc
     9541 ataccgggtt gccgccaaac actgcgcccg gacgacgaaa gcaccctgaa agccaatgcc
     9601 cgcatcaggg aattggcagc caaatacccc aacgtctata ttattgatgc cgccgcctat
     9661 atccccgcag atttccaaat cggcggattg ccggtttact cggacaaaga ccacatcaac
     9721 ccttacggcg gcacagaatt ggcgaagcgt ttttccgaaa aacaaaggtt tctcgatacg
     9781 cgccataacc attgattcgc ttaaatttgt tacaatcggc ggtttgcaaa accgctaatt
     9841 tttttgaaag agaccgatga gcgtcatcca agacctgcaa tcgcgcggcc ttatcgcgca
     9901 aaccaccgac atcgaagcct tagacgcttt gctgaacgaa caaaaaatcg ccctttactg
     9961 cggtttcgac ccgacagccg acagcctgca catcggacac ctgctgcccg tattggcatt
    10021 gcgccgcttc caacaggcgg ggcatacgcc gattgcactg gtgggcggcg cgaccggtat
    10081 gatcggcgac cccagcttca aagccgccga acgcagcttg aattccgccg aaaccgttgc
    10141 cggctgggta ggaagcatac gcagccaatt aacccctttc ttgagctttg aaggcggaaa
    10201 cgccgccatt atggcgaaca atgccgactg gttcggcagc atgaactgcc tcgacttcct
    10261 gcgcgacatc ggcaagcatt tctccgtcaa cgccatgctg aacaaagaat ccgtcaaaca
    10321 gcgcatcgac cgcgacggcg caggcatttc cttcaccgag ttcgcctatt ccctgctgca
    10381 aggctacgac ttcgccgagt tgaacaaacg ccacggcgcg gttttggaaa tcggcggttc
    10441 cgaccagtgg ggcaacatca ccgccggtat cgacctgacc cgccgcctga accaaaaaca
    10501 agtgttcggt ctgaccctgc ctttggttac caaatccgac ggtaccaaat tcggcaaaac
    10561 cgaaggcggc gcggtgtggc tgaacgcgaa aaaaacctcg ccgtaccagt tctaccagtt
    10621 ctggctgaaa gtcgccgatg ccgatgtgta taaattcctg aaatacttta ccttcctgtc
    10681 catcgaagaa atcggtgtcg tcgaagccaa agacaaggca agcggcagca agcccgaagc
    10741 gcaacgcatc ctcgccgaag aaatgacccg cctgattcac ggcgaagaag cccttgccgc
    10801 cgcgcaacgc atttccgaaa gcctgtttgc cgaagaccaa agccggctta ccgaaagcga
    10861 cttcgagcag ctcgccctcg acggcctgcc tgcatttgaa gtttcagacg gcatcaacgc
    10921 cgtcgaagcc ttggtcaaaa ccggcttggc agcgtccaac aaagaagcgc gcggctttgt
    10981 caatgccaaa gcggttctgc tcaacggcaa accggctgaa gccaacaacc ccaaccacgc
    11041 cgccgaacgc cccgacgatg cctatctgtt gataggcgaa tacaaacgtt tcggcaaata
    11101 caccatcctc cggcgcggca aacgcaacca cgcgcttttg gtttggaaat aatccgattg
    11161 ccgcagaaat gccgtctgaa gctttcagac ggcattttta tcaaatgcaa aacaccctgc
    11221 gcctgccgat atgtcgtcat ttccatgcag gcgggaattc aaacttgtcc gcacggaaac
    11281 ttatcgggca aaacggtttc ttcagttcta cgttctagat gcccgctttc g
//
LOCUS       DAWTCI010000065        11061 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWTCI010000065 DAWTCI010000000
VERSION     DAWTCI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.065"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            170..2543
                     /locus_tag="WM481_001643"
                     /pseudo
     CDS             170..2543
                     /locus_tag="WM481_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(2752..3225)
                     /locus_tag="WM481_001644"
     CDS             complement(2752..3225)
                     /locus_tag="WM481_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFA9080918.1"
                     /translation="MLAACQSGRALKNAHTASAGTPAGESCKVIRGDTAGGGKNIIYR
                     CSNGQAAINAATAAGVLESGIPVSFGGGVPVANGRNLVTRQAANGVGKSDSEACEHAL
                     INAARKFQQTAGKLGGRSVTGFHSYFGKQSLQGGQYDCQAGSFHVRAVMRANVVR"
     gene            complement(3356..4711)
                     /gene="yegQ"
                     /locus_tag="WM481_001645"
     CDS             complement(3356..4711)
                     /gene="yegQ"
                     /locus_tag="WM481_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFA9080919.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVADMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELGMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESEAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIHDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            5037..5825
                     /locus_tag="WM481_001646"
     CDS             5037..5825
                     /locus_tag="WM481_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFA9080920.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDTGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPGVDEDGVLRGLQDLGVI"
     gene            complement(5871..6470)
                     /locus_tag="WM481_001647"
     CDS             complement(5871..6470)
                     /locus_tag="WM481_001647"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFA9080921.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            6598..7215
                     /gene="gmk"
                     /locus_tag="WM481_001648"
     CDS             6598..7215
                     /gene="gmk"
                     /locus_tag="WM481_001648"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFA9080922.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSTEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLKGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLEKAEGDLLHIVNACRLKRSRQLGFIADLLENS"
     gene            7273..7479
                     /locus_tag="WM481_001649"
     CDS             7273..7479
                     /locus_tag="WM481_001649"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFA9080923.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            7572..9728
                     /locus_tag="WM481_001650"
     CDS             7572..9728
                     /locus_tag="WM481_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFA9080924.1"
                     /translation="MPAPQPSAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGDPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYRELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGTLHTLYQPKPGRFKDYIAIPKSNGYQSLHTILVGPYGLPIEVQ
                     IRTREMDAVAEGGIVGHWSYKSDSKTVDQAVLHTNQWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARVNNVMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="WM481_001651"
     CDS             complement(9795..10979)
                     /gene="coaBC"
                     /locus_tag="WM481_001651"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFA9080925.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACRENGM
                     GRMPEPAELLDLLPDLWTPKILRDKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEISLIHGQLQTTLPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDTLASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKTTNQIVIIDDDAELSFPETSKDEAAMRIVERLAVYL
                     NK"
BASE COUNT         2675 a         2667 c         2998 g         2721 t
ORIGIN      
        1 aaataatgaa acaaacaagg cctcggcata gatgtttgca gagaccttta attacacgac
       61 acagatttat ttgtacgggt tgctgtctta ttaaatatta atgattatca gaaaatttat
      121 tatgaattaa ctgatggatg aacaatccat tacatcttga gttgataata tgaaaccatt
      181 acacatgctt cctattgccg cgctggtcgg cagtattttc ggcaatccgg tcttggcagc
      241 ggatgaagct gcaaccgaaa ccacacccgt taaagcagag ataaaagaag tgcgcgttaa
      301 agaccagctt aatgcgcctg caaccgtgga acgtgtcaac ctcggccgca ttcaacagga
      361 aatgatacgc gacaacaaag acttggtgcg ttactccacc gacgtcggct tgagcgatag
      421 cggccgccat caaaaaggct ttgctgtgcg cggcgtggaa ggcaaccgtg tcggtgtcag
      481 cattgacggc gtgagcctgc ctgattcgga agaaaactca ctgtatgcac gttatggcaa
      541 cttcaacagc tcgcgcctgt ctatcgaccc cgaactcgtg cgcaacatcg aaatcgcgaa
      601 gggcgctgac tctttcaata ccggtagcgg cgcattgggt ggcggcgtga attaccaaac
      661 cctgcaaggc catgatttgc tgttggacga caggcaattc ggcgtgatga tgaaaaacgg
      721 ttacagcagc cgcaaccgcg aatggacaaa tacactcggt ttcggtgtga gcaacgaccg
      781 cgtggatgcc gctttgctgt attcgcaacg ttgcggtcat gagaccgaaa gcgcgggcga
      841 gcgtggctat ccggtagagg gtgctggcag cggagcgaat atccgtggtt cggcacgcgg
      901 tatccctgat ccgtccaaac acaaatacca caacttcttg ggtaagattg cttatcaaat
      961 caacgacaag caccgcatcg gcgcatcgtt taacggccag caggggcata attacacgat
     1021 ttgaagagtc ttataacctg accgcttctt cctggcgcga agccgatgac gtaaacagac
     1081 ggcgcaatgc caacctcttt tacgaatgga cgcctgattc aaattggttg tcgtctttga
     1141 aggcggactt cgattatcag aaaaccaaag tggcggcggt taacaacaaa ggctcgttcc
     1201 cgatggatta ttccacctgg acgcgcaact ataaatcaga aggatttgga cgaaatatac
     1261 aaccgcagca tggacacccg attcaaacgt tttactttgc gtatggacag ccaaccgttg
     1321 caactgggcg gccaacatcg cttgtcgttt aaaactttcg ccagtcggcg tgagtttgaa
     1381 aacttaaacc gcgacgatta ttacttcagc ggacagatat cccgtactac cagctcgatt
     1441 caacaccctg tgaaaaccac taattatggt ttctcactgt ctgatcaaat ccaatggaac
     1501 gacgtgttca gcagccgtgc agatatccgt tacgatcata ccaaaatgac gcctcaggaa
     1561 ttgaatgccg agtgtcatgc ttgtgacaaa acaccgcctg cagccaatac ttataaaggc
     1621 tggagcggat ttgtcggttt ggcggcgcaa ctgaatcagg cttggcatgt cggttacgac
     1681 attacttccg gctaccgtgt ccccaatgcg tccgaagtgt atttcactta caaccacggt
     1741 tcgggtaatt ggctgcccaa tcccaacctg aaagccgagc gcagcaccac ccacaccctg
     1801 tctctgcaag gccgcagcga aaaaggtact ttggatgcca acctgtatca aaacaattac
     1861 cgcaacttct tgtctgaaga gcagaagctg accaccagcg gcgatgtcgg ctgtactcag
     1921 atgaattact actacggtat gtgtagcaat ccttattccg aaaaaccgga atggcagatg
     1981 caaaatatcg ataaggcccg aatccgtggt cttgagctga caggccgtct gaatgtggac
     2041 aaaatagcgt cttttgttcc tgagggctgg aaattgttcg gctcgctggg ttatgcgaaa
     2101 agcaaactgt cgggcgacaa cagcctgctg tccacacagc cgccgaaagt gattgccggt
     2161 gtcgactacg aagcccgagc gaaaaatggg gtgtgttctc ccgcctgact tatctgggtg
     2221 cgaaaaaggc caaagacgcg caatacaccg tttatgaaaa caagggccgg ggtacgcctt
     2281 tgcagaaaaa ggtaaaagat tacccgtggc tgaacaagtc ggcttatgtg tttgatatgt
     2341 acggcttcta caaaccggct aaaaacctga ctttgcgtgc aggcgtatat aatgtgttca
     2401 accgcaaata caccacttgg gattccctgc gcggtttgta tagctacagc accaccaacg
     2461 cggtcgaccg agatggcaaa ggcttagacc gctaccgcgc ctcaggccgt aattacgccg
     2521 tatcgctgga atggaagttt taatctagta ttattggatt aattgcctcg ttggggatta
     2581 aagctgtccg aattgttttc aggaactcat tcggacggct ttttaccgta aatctgtgtg
     2641 atggggtttt atattgatac agtatccgaa ctcagggcag ggagtaatgt tgcaggatta
     2701 aaaatgccgt ctgaaaaatg tttcagacgg cattttttga ttgccgatat gttaacggac
     2761 gacattggcg cgcatgaccg cgcggacgtg gaacgaacct gcctgacagt cgtattgtcc
     2821 gccttgcagc gattgcttgc caaagtagct gtggaagccg gtaacactgc gcccgccgag
     2881 tttgccggcg gtctgctgga atttgcgggc ggcattgatt agggcgtgtt cgcaggcctc
     2941 ggagtcggat ttgccaacgc cgtttgccgc ttgccgtgta actagattcc tgccgtttgc
     3001 aaccggtacg ccgccgccga agctgacggg aatccctgat tccaatacgc cggcggcggt
     3061 ggctgcattt attgccgcct gaccgttgct acaacggtaa atgatgtttt tgccgcctcc
     3121 tgcggtgtct ccgcggataa ctttgcatga ttctccggcc ggtgtcccgg cggatgccgt
     3181 gtgcgcgttt ttaagcgcgc ggccggattg gcaggctgcc agcatcaggg cggcggcgga
     3241 gagtatgagg attttttatg tcggatgtcc ttgtttgctg tgggaaatca ttgccattgt
     3301 acagtaagat tcaacaaaat gccgtctgaa aacggggttc ggacggcatt gtggctcagg
     3361 ggttcaacac gcgtgcgatg agggcttttt ctttgccctg catattgggg attttgacct
     3421 gaatgccgtt gcccggggca acatcgacag gttggccttt gcgggtcatt tgttccaatt
     3481 tgatggtttg gttgccgctc gggtggatga tttcgagcga atcgccgacg gcgaagcggt
     3541 ttttaacttc gatggtcgcc cagccatttt cgtcgatttc ggtaacgtgt ccgacgtatt
     3601 ggctttgttt ggcggtggaa tggccgctga ggtagttttg ataatcctga gtttggtggc
     3661 gttcgaggaa gccgctggtg tagcctcggt tggcaaggcc ttcgagttcg ctcaacaggc
     3721 tgtaatcaaa cggacggcct gcgacggcat cgtgaatcgc tttgcggtag gactgggcga
     3781 cgcgggcgac gtagtagaga gacttggtac ggccttcgac tttgaggctg tccacaccga
     3841 ttttggcgag tttttcgacg acttcgatac cgcgaagatc tttggagttc atgatgtagg
     3901 tgccgtgttc gtcttccata atcggcatca tctcgcccgg gcggttggat tcttcaatca
     3961 ggaaaacttt gtcggcgtag ggatggcgtt tttgaccgtt gatgccttca aagttttggt
     4021 tggcttcttc ttgggctttt tcaaagttga aaccttgcag aagctgggca tcgcctgcct
     4081 cgctttccgt ggcgttgtgg actttgtagt cccaacggca ggagttggtg caggtgcctt
     4141 ggttgggatc gcggtggttg aaatagcccg acaacaggca gcggcctgag taggcgatgc
     4201 acaatgcgcc gtggatgaag acttcgagtt cgatgtcggg gcattcttgg cggatttcgg
     4261 caatttcttc catgcccaat tcgcgtgaca ggatgatgcg ttcgacacca atgttctgcc
     4321 aaaatttcac gccccagtag ttggtggtgt tcgcctgtac ggacagatgg atcggcattt
     4381 ccggccattt ttcgcgcacg gtcataatca aacccggatc cgccataatc agcgcgtcgg
     4441 gtttcatggc aatcagcggc tccatgtcgg caacgaaggt tttgagtttg gaattgtgcg
     4501 gcagggtgtt gacggtcaaa aagaattttt tgttgcgttc gtgcgcttct ttaatgcctt
     4561 gctcgaggac gtcgagtttg gcaaattcgt tgttgcgggc gcgcagggag taacgcgggc
     4621 tgccggcgta aacggcatct gcgccgtaat cgtaggcggc gcgcattctt tccaatccgc
     4681 cggcgggcaa taagagttcg ggtgctttca tcgtgtttcc ttttcggttg aaaccccgcc
     4741 ctttagggcg gcaggatcag actttgtttg ggaggggcgt aaccccttcc gaatcaggac
     4801 gacataatag ggcggtgctt tatgtgtcgt cctgtgtgtt gaagcatgat ttgtatgctt
     4861 tgtaaaaatg tttcagacgg cattgctgca aatgccgtct gaagggtgtt tgaagatggg
     4921 cggattatcc gccttttcgt gttcgcggtc aattttgccg ggaaatttat aaggatttat
     4981 caagtatttg ccttcttggg atacaatgcg gcagtttgag tgcgagagag aatatgatga
     5041 atcccaaaat cgtgtttttc gacattgacg atacgctgta ccgcaaatat acggatacct
     5101 tgcgcccttc cgtgaaaacg gcggtggcgg ctttgcgcgg caaaggtata ttgacggcgt
     5161 tggcaacggg gcggtctttg gcgacgattc ccgaaaaggt cagggacatg atggcggaaa
     5221 cgggtatgga tgccgtggta acgataaacg gacagtttgc gctgctgcac ggtaaaactg
     5281 tgcgcgaagt accgatggat accggtttga tgggcagggt ttgtgcgcat ttggatggct
     5341 tgggcatgga ttatgcgttt gtcggcggag aggggatcgc cgtgtccgcg ctgtcggaat
     5401 gcgtgtgccg cgccttgcag catatcgcca gcgatttttt tgccgataag gattattttt
     5461 caagcaaacc ggtgtatcag atgctggtgt ttgcggagga aaacgaaatg ccgctttggt
     5521 cggatattgt ggaacgggaa ggcttgaaaa cggtgcgctg gcacgaggaa gcggtcgatc
     5581 tgctgcctgc gggaatgtcg aaaacagacg gcatcagaag cgtggttgaa gcattgggtt
     5641 tggaaatggc agacgtgatg gcgttcggcg acggtttgaa cgatgtggaa atgctgtctg
     5701 aagtcgggtt cggcgtggca atgggtaacg gggaacaggc agcgaaagaa gcggcgaaat
     5761 atgtttgccc cggcgttgat gaagacggcg tgttgagggg cttgcaagat ttgggtgtga
     5821 tttgaacgta taataacaac cctgccggtt tcagacggca gggtcggttt tcagcctttc
     5881 atgcagcctt cgttttgaag cagggtaaat aagggcgcgc cacttgcgcg gatattcttg
     5941 ccgccttgaa ggtcggtaaa ttccaaaatg gcggcggctt cgacaatttc cccgccgagt
     6001 ttgcggatca gttccagccc ggcaagcatt gtgccgcccg tggcaaccaa atcatcgacc
     6061 agcaggacgc gcgaaccggg tttgacggca tcggtgtgga tttccaccgc agcttccccg
     6121 tattcgagcg cgtagctttg cgatacggtt tcaaaaggca gcttgccttt tttgcggatg
     6181 gggacgaagc cgacgttgag ctggtaggcg agtgccgcgc cgataatgaa gccgcgcgcg
     6241 tccaagccgg caacgatgtc gattttctga tccatatagc ggtaaaccag caaatcgacc
     6301 aaaaggcgga agtattccgc actttgcagg acgggcgtga tgtcgtggaa taagatgcct
     6361 ttttgcggcc agttttcgat tttgcggatt ttgtcggcaa gcgcgccgac actcatagct
     6421 tcgggatgaa ccagcattgc gtgttccaag tttgatgttt ttaacgccat attgtagccg
     6481 tcagcctgcc gcttgccaaa tcaatcaaaa caatagcggc aaaatatggg catcggcaaa
     6541 tatgaaatag ggtgtccgtt gtgatacaat cggttggaaa atatacaggt aaaaaatatg
     6601 tccgcttatc gaaaaggcaa catctttatc atttcggccg cttccggcac gggcaaaacc
     6661 acgctggtgt cgcggctgtt ggcaaaccat aacggtttgc gcgtttccgt gtcgcatacg
     6721 acgcgcccgc cgcgtgaagg cgaagcaaac ggcgtacatt atcactttgt ttccaaagaa
     6781 gagtttgaat cgcttatcgc gcaggaggct tttttggaat acgcagacgt attcggcaac
     6841 tattacggca caagcacgga gggcgtgaat gcgttggcgg cggcaggcta tgacgtgatt
     6901 ttggaaatcg acgttcaggg cgcggcgcag gttcgcaacg cgctgccgga agctgtcggt
     6961 atctttatcc tgccgccttc tttcgacgtg cttgccgcgc gcctcaaagg gcgcgggacg
     7021 gacagtcggg aagttatcca aaggaggctg tcgaaggcaa ggcatgaaat cgaacagtcc
     7081 gtattgttcg actttgtcgt ggtcaatgac gacctggaga aggcggaggg ggatttgctt
     7141 catattgtga atgcctgccg tctgaaaagg tcgcggcaac tgggatttat tgcagatttg
     7201 ttggaaaatt cctaaaaacc gcgaaaatat ccggtttccc aatttaaata tttttgaaag
     7261 aaagcaaata atatggcacg tattaccacc gaagactgta ccggaaaaat ttccaaccat
     7321 tttgacctga cattggtagc ggcccgtcgg gcccgccagc ttgaaaatgg caatacgccg
     7381 cttgtggacg atgtccgcaa taacaaaccg accgttaccg ccttaaggga aatcgcagcc
     7441 gggcatatcg gtacggaact gttgacgcgc aataaataaa ttctgccgga aacgcacgcc
     7501 ggcacactct gccgccgtgc agtccgacgg tttgaaatga aaaaacacat acatcccgaa
     7561 agccgtctga aatgcccgcc ccccaacctt ccgcccctta cgaccccctg accgccgaag
     7621 cgcgtaccct gcttttccat accgcctcct acctcaagcc cgaggaacag gcggagcttg
     7681 aaaaagctgt cgcctatgcg tttcgcgccc acgacgggca aacccgcaaa agcggggatc
     7741 cctacatcac gcatccgatt gccgttgcga cgcagctcgc cctttggcat atggacatac
     7801 agggtctttg tgcaggcgtg atgcacgacg tattggaaga tacgggcgtg acaaaagggg
     7861 aaatggcggc ggtgttcggc aatactgttg ccgagatggt ggacgggctg tccaaactcg
     7921 aaaaactcaa atttgaagat catgcggaac atcaggcgga gagtttccgc aaactgattt
     7981 tggcgatgac caaagatgtg cgcgtcatcg ttgtcaaact tgccgaccgc ctgcacaata
     8041 tgcggacgct cggttcgatg cgcccggaca aacgccgccg gattgcaagg gaaacccttg
     8101 aaatctacgc acagattgcc aaccgcatag gtctgaataa tgcgtatcgg gagcttcagg
     8161 atttatcgtt ccaaaacctg catcccaacc gctacgagac tttaaaaaaa gcgatggaca
     8221 agagccggaa gaacaggcag gacgttgtcg gcaaagtttt gcgcgcgttc ggccagcggc
     8281 tggtaggcgc gaatatagag gccaaaatca aaggcaggga aaaaaacctg tacggcatcc
     8341 atcagaaaat gatggcgaaa aagctgcgct ttgccgaggt tatggatatt tacggtttcc
     8401 gcgtcattgt caacagcatt cccgcctgtt atgccgcact cggcacattg cacaccctct
     8461 atcagcccaa gcccggacgg ttcaaagact atatcgccat tccgaaaagc aacggttatc
     8521 aaagtctgca tacgattttg gtcggcccct acggcttgcc gattgaagtt cagatacgca
     8581 cgcgggaaat ggatgccgtg gcggaaggcg gtattgtcgg ccattggagc tataaatcag
     8641 attccaaaac ggtcgatcag gcagtgctgc acaccaatca atggttgaaa aacattttag
     8701 acttgcaggc aagcagcgcc aatgccattg agtttctcga acacgtcaaa gtcgatttgt
     8761 ttccgaacga aatctacatc cttactccga aaggaaaaat cctaactttg cccaaagggg
     8821 caacgcctgt cgattttgct tatgcggtgc ataccgacat tgggcataaa acagttgccg
     8881 cgcgtgtcaa taatgtgatg atgccgctgc gtacgaagct caaaaccggt gattctgttg
     8941 aaattatcac atccgaacac gccaaaccca atcccgcgtg gttgaatttc gccgtgtccg
     9001 gcagggcgcg cagtgccata cgccaatata ttaaaaatct taaccggcac gatgcggtcg
     9061 ttttgggaga gagcctctta caaaaagcct tgtccagttt gctgcccaaa gatgtcctgc
     9121 tttcagacgg catcaaagaa aaataccttg ccgatctcaa cgacaaacag acttcatttg
     9181 aggaagtgct gtacaacgtc ggtatggggc ataccctgcc tgtttatgtc gccatgcata
     9241 tcgccgaatt ggcaggggag catttcggca gcgaggtcag gctcagttcc attaaagttg
     9301 atggtcagga aagcgggcat attcattttg cagagtgctg ccaccctgtt cccggcgatt
     9361 ccatccgttt gctgttggtt aagggaaaag gcatgattat ccatagggat acctgcccga
     9421 cgttgttgaa gtccgatccc gaacagcagc tggatgcaga ctgggaaaat atgaacgggc
     9481 agaactaccg tgtcgggctt caagtccaat cggaagacag ccacggcctg ttggcattaa
     9541 tggcgcaagc gatttccgat tccggtgcgg acatcgagtc ggtcgaaaca ccgtctaaat
     9601 cccagtcggg aacggaaggt tttgtcgaat tcaaattctt attgaaagtc aagaatttgg
     9661 atcagttgaa tcagattatt caaaatctgc acagcatccc atatatccgc aaagtcatca
     9721 gaagttgaag caggtaaaac gggttgcctg ttaatcaaaa ggcaacccgt tttatggttt
     9781 atccgttcaa ttgcttattt gttcaaatat acggcaagcc tttcaacaat ccgcattgcc
     9841 gcttcatctt tacttgtttc ggggaaagac aactccgcat catcatctat gataacaatt
     9901 tggttggttg tcttgcccat cgcgattgaa acatcattgg caactatcac cggtattttt
     9961 ttcttaatac gtttttcccg cgcatatgcc attacattct ccgtttcagc ggcaaaaccg
    10021 atgcagaacg gcgggttcgg caatgaggca atagaagcca aagtatcggg gttctcatcc
    10081 aattcgatgg ataacggttt ggcattttta tcttttttga atttttgcgt actcctgttt
    10141 ttaaccttat agtctgagac ggcggcaaca gaaataaaag catcttgttt ttcaatcaaa
    10201 cgatgtactg cgcgatgcat atcttcggca ctgacggctt gaaccgtatc ggatatgccg
    10261 aaaggcagcg tggtttgaag ctgtccgtga atcaggctga tttccgcacc ggcggcacgg
    10321 cacgcccgcg ccaaagctac gcccattttt ccgctggagg tatttgtgat gcccctgaca
    10381 gggtcaatgg cttcaaatgt cgcacctgcg gtaatcaaga ttttcttgtc ccttaaaatt
    10441 ttcggtgtcc acaagtctgg aagcaaatcc agcaattcgg caggttccgg catccttccc
    10501 ataccatttt ctctgcaagc ctgttcgccc aagcccggca tatagacagt aatgccgtct
    10561 gaaaccagtt gtgcgatatt ccgttggttg gcaggattca gccacatttc cacattcatc
    10621 gcgggagcga tggcaagagg gcatttccgt gcggctgcca gattggtcag taggttatct
    10681 gccacgccgt tacagatttt tgccacggta ttcatacttg ccggcgcaat cagaaaaaca
    10741 tccgcattcc gggtcaggtt gatatgttcc ataccgtttg aaccgttgcc gccgtgcgtg
    10801 tcggtcagaa cgggattgcc gctcaaagcc tgaaaagtca gcggagaaac aaattcagtt
    10861 gccgagcggc tcataaccac cgtaaccgaa tgcccctgtt ttttcagcag tcgcaccaac
    10921 tcgcaagact tatacgccgc aatactgccc gttacaccta aaagaatatg tttgcccatt
    10981 ttgacaattt ctaattacga agaaaaaaca gcagccattc tataacaaag tacggataca
    11041 ttagttaccg tttggatacg a
//
LOCUS       DAWTCI010000066        11009 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWTCI010000066 DAWTCI010000000
VERSION     DAWTCI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.066"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(54..1082)
                     /gene="trpD"
                     /locus_tag="WM481_001652"
     CDS             complement(54..1082)
                     /gene="trpD"
                     /locus_tag="WM481_001652"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFA9080926.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMCEFASKVPLEDADGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNIAASLSDGISAAREGIDSGRAKAKKEEF
                     VGFTRQFA"
     gene            complement(1146..1736)
                     /locus_tag="WM481_001653"
     CDS             complement(1146..1736)
                     /locus_tag="WM481_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFA9080927.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVEAMRHFAGRLPIMGVCLGHQTIGEAFGGDVVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTERGHDMLNNFLVEFQNFKPQKI"
     gene            2147..4423
                     /locus_tag="WM481_001654"
     CDS             2147..4423
                     /locus_tag="WM481_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9080928.1"
                     /translation="MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNKTHNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPHHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLAAYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDPYGKRPFIAQDDQNAPRIPAARLGFHLKTSLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            complement(4963..5742)
                     /locus_tag="WM481_001655"
     CDS             complement(4963..5742)
                     /locus_tag="WM481_001655"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFA9080929.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDVYLPVDAQAQVAIGDKVTGVKTVLARLPLTDS
                     QADPVSQAASVETAANPSAEQQQIEAAAAKIQAAVQDVLKD"
     gene            5896..8745
                     /gene="uvrA"
                     /locus_tag="WM481_001656"
     CDS             5896..8745
                     /gene="uvrA"
                     /locus_tag="WM481_001656"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFA9080930.1"
                     /translation="MCNHHPRHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILGPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     HHYKFDVNVAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCEKSVTGQYLGGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGKVIAKGSPEQVAKIKGSYTGKYLKVVLK"
     gene            8763..9404
                     /locus_tag="WM481_001657"
     CDS             8763..9404
                     /locus_tag="WM481_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="HFA9080931.1"
                     /translation="MNNNFDRSKHIYHNEAFVELVKDAIRFFHGTPVHTLPPPKQFKG
                     AGVYAIYYIGNNPLYKQYADWNRLSYNAPIYVGKAVPKGWRQARNSDNALNQSTELFH
                     RLKEHSRSIAAVSDLDPSDFMCRFVIFEGAGSDMIGTIEAALIKLHKPLWNSCVDGFG
                     NHDPGKGRYEQAKSDWDVLHSGRVWADRLNGIPNSYESILENINTHLEIIKRK"
     gene            9408..10541
                     /gene="dcm"
                     /locus_tag="WM481_001658"
     CDS             9408..10541
                     /gene="dcm"
                     /locus_tag="WM481_001658"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFA9080932.1"
                     /translation="MKSLEIFSGAGGLAKGLELAGFQHASFIELNKDACNSLRSNFNP
                     KLVYQGDVADFDLSSQEGIEVIAGGPPCQPFSLGGKHLAHEDRRDMFPHAVRYVEYYR
                     PKAFIFENVKGLLRKSFADYFEYILLRLTYPNLGILQNEDWKGHLTRLKEIEFNLYKG
                     IKYKVSYQLLNAADYGVPQKRERVVIVGIRADLDIDWKFPKRTHSEDRLNWEKYVTGE
                     YWEKHNEPKRFNKDIAEKLQKKYGIFEPEKKPWQTVRDTLSDIPHPLGNHKITGHEYR
                     DGARIYPGHTGSGIDEPSKTIKAGGHGVPGGENMIRYDDGTVRYFTSYEAKLLQTFPE
                     EFVISGAWGEAMRQIGNAVPVKLSEILGKHLMGVLSEKSSLHN"
     gene            10849..>11009
                     /locus_tag="WM481_001659"
     CDS             10849..>11009
                     /locus_tag="WM481_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9080933.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPHTK"
BASE COUNT         2907 a         3099 c         2556 g         2447 t
ORIGIN      
        1 cgccaattcc gccgttcaga cggcatttgt tgctttatat caagtttgcc ggcttaggcg
       61 aattgccgtg taaaaccgac aaactcctct tttttcgcct tcgccctacc tgaatcgatg
      121 ccttcccgtg cggcagatat gccgtctgaa agcgaagcag cgatatttcc ggcatataag
      181 gcggcggcgg tattaagcaa tacgatatcg cgcgccgccc cttctttccc gtccagtacc
      241 tcgttcattt tcaacaaaga ttcctgagta ttggcaactt tgatttcatc caaattgcgg
      301 cgggtttcga taccgaaatc ttctgggcgg atgtcgtatt cgctgatttt tccgtctttg
      361 agttcggcaa cgcgcgtttt gcccgtcagt gtaatttcat ccaaaccgcc ctccccgcaa
      421 acgaccaaaa cgtgttttga gccgagttgt tgcaagaccc gcgacaaaat gccgcacaaa
      481 tcggtgtgga acacgcccaa aagctggttc ggcgcgcccg caggattggt taacggaccc
      541 aatatgttga aaatgcttcg gaaaccgagc gaacggcgca ccggggcgac atggcgcatg
      601 gcactgtggt gattgggtgc gaacataaac cctataccgg tctgcctgat actttgggca
      661 atctgttcgg gagtcaggtt gaggtttgcg cccatttgct ccatcacgtc agccgcaccg
      721 ctggaggaag agaccgaccg gcctccgtgt ttggcaacct ttgcgcctgc cgctgcagca
      781 acaaacatcg aagtcgtcga aatattgaag gttttcgcgc catccccgcc cgtaccgacg
      841 atatcgacca gcccgtccgc atcctccagc ggcacttttg acgcaaactc gcacatcacg
      901 gcggcggcgg cggtgatttc ggaaacggtt tcaaccttga tacgcaagcc ggtcaaaatc
      961 gccgcaattt gttcgggcgg cacttttccg ctcatcattt ggcgcatcaa gtcggtcatt
     1021 tcatcgtaaa acaactcgtt attgctgatt aatcgttcga tagcctgttg cggtgtaatc
     1081 attttttgtc ctccgttcaa tattcggacg aaaatgccgc ccgaagggct tcagacggca
     1141 tcacgtcaga ttttttgcgg tttgaagttt tgaaattcaa ctaaaaaatt gttcaacata
     1201 tcatgtccgc gttcggtcaa gagggcttcg gggtggaact gcacgccctc gacggcatat
     1261 tccttatggc gcacgcccat aatttcgccg tcttccgtcc acgccgtgat ttccaagcaa
     1321 tccggcagcg tgccgcgttc gataacgagg ctgtgataac gcgtacaggt aaccggattg
     1381 ggcaaaccct taaacatacc cttgcccgaa tgggacacgg gcgacacctt accgtgcatc
     1441 aaggtttttg cccgtaccac atctccaccg aacgcttcgc ctatcgtctg atgcccgagg
     1501 cacacgccca taatcggcag ccggccggca aaatggcgca tggcttctac tgaaataccc
     1561 gcctccttag gggaacacgg tccgggaccg ataacgagat attgcggatt caatgcctcg
     1621 atttcctcca acgtaatatc atcgttgcgg cgcacggcga cttcctgccc caattctgcg
     1681 aaatactgga cgatgttgta agtaaaactg tcgtaattgt cgataaacaa aagcattttg
     1741 tgattaacct attgatttat aatttgttta ttattaactg catttccgat acccccatcc
     1801 ataccccctt atatcttagc gtgcccgatg cgccctcgtg aatctggcgc agagcctcgc
     1861 gttctgacaa gtacgccatt ttgccacctt ttcgaccgtt cgggaacgta ccgtttttat
     1921 gccctcataa attttggtta tccgatagct aacatatagc ggcgttcgtg ttcacatcat
     1981 taacttccct aaaattaggg ttaatacaaa ttaacgccat taaatatcac aaccgtgtca
     2041 tttgaaaaac ttttgcctat gccctctgag tattctcaaa atcttatgat ataaaaaatg
     2101 caatgttata taataacaaa cttttgaaat gcgacgagaa gccaaaatgg cacaaatcac
     2161 actcaaaccc attgttttat caattctttt aatcaacaca cccctcctcg cccaagcgca
     2221 tgaaactgag caatcggtgg gcttggaaac ggtcagcgtc gtcggcaaaa gccgtccgcg
     2281 cgcgacttcg gggctgctgc acacttcgac cgcctccgac aaaatcatct ccggcgatac
     2341 tttgcgccaa aaagccgtca acttgggcga cgctttggac ggcgtaccgg gcattcatgc
     2401 ctcgcaatac ggcggcggcg catccgctcc cgttattcgc ggtcaaacgg gcagacggat
     2461 taaagtattg aaccatcacg gcgaaacggg cgatatggcg gacttttctc ccgatcacgc
     2521 cattatggta gataccgcct tgtcgcaaca ggttgaaatc ctgcgcgggc cggttacgct
     2581 cttgtacagc tcgggcaatg tggcggggct ggtcgatgtt gccgatggaa aaatccccga
     2641 aaaaatgcct gaaaacggcg tatcgggcga agccggattg cgtttgagca gcggcaattt
     2701 agaaaaactg acatccgcag gcatcaatat cggactgggc aaaaacttcg tgctgcatac
     2761 cgaaggcttg taccgcaaat cgggcgatta cgccgtaccg cgttaccgca atctgaaacg
     2821 cctgcccgac agccatgccg attcgcaaac gggcagcatc gggctgtctt gggtaggcga
     2881 aaaaggcttt atcggcgcag catacagcga ccgtcgcgac cgctacggcc tgcctgccca
     2941 cagccacgaa tacgatgatt gccacgccga catcatctgg caaaagagtt tgatcaacaa
     3001 acgctatttg cagctttatc cgcacttgtt gaccgaagaa gacatcgatt acgacaatcc
     3061 gggcttgagc tgcggcttcc acgacggcga cggtgcacac gcacacaccc acaacggcaa
     3121 accgtggata gacctgcgca acaaacgcta cgaactccgc gccgaatgga agcagccatt
     3181 ccccggtttt gaagccctgc gcgtacatct gaaccgcaat gactaccacc acgacgaaaa
     3241 agcaggcgat gcagtagaaa acttcttcaa caacaaaaca cacaacgccc gtatcgagtt
     3301 gcgccaccaa cccataggcc gtctgaaagg cagctggggc gtgcaatatt tgggacaaaa
     3361 atccagcgcg ctttccgcca ttcccgaaac cgtccaacaa ccgatgttga ttgacaacaa
     3421 tgtccgccat tacagctttt tcggtgtaga acaggcaaat tgggacaact tcacgcttga
     3481 aggcggcgta cgcgtggaaa aacaaaaagc ctccatccgg tacgacaaag cattgattga
     3541 tcgagaaaac tactacaacc agcccctgcc cgacctcggc gcgcaccgcc aaaccgcccg
     3601 ctcgttcgca ctttcgggca actggtattt cacgccacac cacaaactca gcctgaccgc
     3661 ctcccatcag gaacgcctgc cgtcaacgca agaactgtac gcacacggca agcacgtcgc
     3721 caccaacacc tttgaagtcg gcaacaaaca cctcaacaaa gagcgttcca acaatatcga
     3781 actcgcgctg ggctacgaag gcgaccgctg gcaatacaat ctggcagcct accgcaaccg
     3841 attcggcaac tacatttacg cccaaacctt aaacgacgga cgcggcccca aatccatcga
     3901 agacgacagc gaaatgaagc tcgtgcgcta caaccaatcc ggtgccgact tctacggcgc
     3961 ggaaggcgaa atctacttca aaccgacacc gcgctaccgc atcggcgttt ccggcgacta
     4021 tgtacgaggc cgtctgaaaa acctgccgtc cctacccggc agggaagatc cctacggcaa
     4081 acgtcccttc atcgcgcagg acgaccaaaa cgccccccgc attccggctg cgcgcctcgg
     4141 cttccacctg aaaacctcgc taaccgaccg tatcgatgcc aatttggact actaccgcgt
     4201 gttcgcccaa aacaaactcg cccgctacga aacgcgtacg cccggacacc atatgctcaa
     4261 cctcggtgca aactaccgcc gcaatacgcg ctatggcgag tggaattggt acgtcaaagc
     4321 cgacaacctg ctcaaccaat ccgtttacgc ccacagcagc ttcctctctg atacgccgca
     4381 aatgggccgc agctttaccg gcggcgtaaa cgtgaagttt taaaatcgga caggcaaaca
     4441 aaaaaggttt tcagacggca ttctgaaaac ctttttttct ttttaatcaa cttgttagga
     4501 aatatggcaa agacaaggaa caaaccactg tgccatcatt cccattttct ttgtttcatc
     4561 attcttgctt tcacgccgtc attctcgcaa aagcagaaaa atcaaaagca aaaacccaag
     4621 atcccatcat tcccgcgcag gcgggaatcc aattcattcg gtttcagccg tttccgataa
     4681 atcaccgtaa cgttaagttc ccggattccc actttcgtgg gaatgacggc gtaaaggttt
     4741 ctgtttttta agttttaggt aactaatgag tcgtcattcc cgcgcaggcg ggaatccaat
     4801 tcgttcggtt tctgttattt ctgataaatc accgtaacgt taagttcccg gattcccatt
     4861 ttcgtgaaca taacggtgtg aacattagca aaaattcaaa aaaccaaact tcctcattcg
     4921 tgtgaatgat ggtactgata ttttctattt cagttcgcaa aattaatctt tcaacacatc
     4981 ttgcacagcc gcctgaatct tagccgccgc cgcttcgatt tgctgctgtt cggcagatgg
     5041 gtttgccgct gtttcaaccg aagcagcttg tgaaacagga tcggcttgag aatcagtcag
     5101 cggcaaacgc gccaatacgg tttttacgcc ggttacttta tcaccaatcg ccacttgcgc
     5161 ctgcgcatcg acaggcagat acacatccac gcgcgaaccg aagcggataa agccatagcg
     5221 ttcgccgcgg gacagtttcg cacctgcttg ggtgtaacac aaaatacggc gtgccaccag
     5281 accggccact tgaacaaaag taatttcacg accggaagcc gtagtcgcca aaaccgcgtt
     5341 gcgttcgttt tccgtgctgg ctttgtccaa atccgcattc acgaatttgc ctttgttata
     5401 gaccactttc gttaccgtac aatcggcagg cgatttttgc gaatgcacgt tgaacacgtt
     5461 cataaaaata ctgattttca aagcatcgac atcacgatac ggatcgcgtg cgcgctcgac
     5521 caccacgata cggccgtcaa ccggactcaa caccgcttca ggattttgcg gaatttcacg
     5581 cgcagggtcg cggaaaaact gcaatgcaaa tacggtaaac acccaaaacg gcaaagacca
     5641 ccagccgcag cacatcgaca ccagcaggct caaagccaaa ccgccgccga taatcggcca
     5701 gccctcacgg gcgataatcg ggtggggata aagacggttc atttgtcatc cttttattca
     5761 aatatattaa atagcgacat tataacggaa tccgccgcgt tccgccgaca aatgccgtct
     5821 gaacgcggct tcagacggca tccgagaaag tgctacaata tcggttttcc tttatttttt
     5881 caggcggcat ccattatgtg caaccatcat ccccgacatt cgcacgacaa cgacaccatc
     5941 cgcattcgcg gcgcgcgcac gcataatttg aaaaacatcg atttggacat tccccgccac
     6001 aagctcgtgg tggtaacagg attgtcaggc agcggcaaat catcgctggc gtttgatact
     6061 ttatatgccg aagggcagcg gcgttatgtc gagagcctgt ctgcctatgc gcggcagttt
     6121 ttgcagatga tggacaaacc cgatgtcgat ttgattgaag gcctgtcgcc tgcgatttcc
     6181 atcgagcaga aatccaccag ccacaatccg cgttccaccg tcggcacggt aacggaaatc
     6241 cacgactacc tgcgcctttt atacgcccgt gtcggcacgc cgtattgccc cgaacacaag
     6301 ctgccgctat ccagccaaac cgtatctcaa atggtcgatg ccgtattaaa actgccggaa
     6361 gatacgcgcg tgatgattct ggggccggcg gtgcgcgagc gtaagggcga gtttgtcgat
     6421 ttctttgccg acttgcaggc gcaaggcttt gcccgtgtgc gcgtggacgg cgaagtctat
     6481 cagcttgacg aagtaccgaa gctggaaaaa aacatcaaac acaatatcga cgtcgtcatc
     6541 gaccgcgtga aagtgaaagc cgacatcaaa caacgactgg cggaaagttt tgaaaccgcg
     6601 ctgcgccacg gcaacgagcg cgcgctggcg atggaaatgg acagcggcga agaacattgg
     6661 ttttccgcgc gttttgcctg ccccgtatgc tcatacagcc tgcccgaatt ggaaccgcgc
     6721 ctcttttcgt tcaacaaccc gatgggttcc tgcccgactt gcgacggctt gggcaacacc
     6781 aatttcttcg accccgaaaa agtagtcgcc catcccgaat tgtcgctggc aaccggcgcg
     6841 attgacggtt gggacaaacg caaccaattc tatttccaaa tgattcaatc gctggcgcat
     6901 cattataaat ttgatgtcaa cgtcgcttgg gaaactttgc ctgaaaaagt caaaaaagtc
     6961 gtgttgcacg gctcgggcaa agaagtcatt gatttcacct acctgtccga acgcggcacc
     7021 accttcaacc gcagccacgc cttcgagggc atcatcccca atctcgaacg ccgctaccgc
     7081 gaaaccgaca gcgaaaccgt gcgcgaaaaa ctgcgcgagt accaaaatca ccgtgcctgc
     7141 ccgagctgcg gcggcgcacg tttgcgcaaa gaagcacgct acgtttacgt cggcggcgaa
     7201 ccgttgcacg aagtctccgc ctggccgctg accaaaaccc accgattctt cgaaacgctg
     7261 gatttggacg gcaacaaaaa acagatcgcc gaaaaaatcc tcaaagaaat caccgagcgg
     7321 ctcggcttct tgattaacgt cgggctggat tacctgaatc taagccgttc cgccgaaacc
     7381 ctttccggcg gcgaagccca gcgcatccgc cttgccagcc aaatcggcag cggcctgacc
     7441 ggcgtgatgt acgttttgga cgaaccctcc atcggcctgc accagcgcga caacgaccgc
     7501 ctgctcgcca ccctcaaacg cctgcgcgat ttgggcaaca gcgtaattgt ggtcgaacac
     7561 gacgaagacg ccatccgcga agccgatttc gtcgtcgata tgggccccgg cgcgggcgaa
     7621 cacggcggca acgtactgat tgccgacacg cccgaaaacg tcgccaaatg cgaaaaatcc
     7681 gttaccggac aatacctcgg cggtaaaaaa tccattgccg tgccgtctga acgcacgccc
     7741 gtcaatcccg gccgaatgct cgtcctcaaa ggcgcgcgcg gcaacaacct caaaaacgta
     7801 accctcgagt tgccgcttgg tttgattacc tgcatcaccg gcgtatccgg cagcggcaaa
     7861 tccaccctga ttaacgacac cctcgccaaa atcaccgccc gcgaactcaa ccgcgcccaa
     7921 gaagaacccg ccccatacga cgacatccgc ggcctcgaac acctcgacaa agtcatcaac
     7981 gtcgaccaat cccccatcgg ccgcaccccg cgctccaacc ccgccaccta caccggcctg
     8041 ttcaccccca tccgcgaact cttcgccggc gtacccctct cgcgcgaacg cggctacaac
     8101 gtcggcagat tctccttcaa cgtcaaaggc ggccgctgcg aagcctgcca aggcgacggc
     8161 gtaatcaaag tcgaaatgca cttcctgccc gacgtgtacg tcccctgcga agtctgccac
     8221 ggcaaacgct acaaccgcga aaccctcgaa atccaataca aaggcaaaaa catcagccaa
     8281 gtcctcgaca tgacggtcga agaagcccgc gaatttttcg acgccgtccc caccgtatcg
     8341 cgcaaactgc aaaccctgat ggacgtaggc ctaggctaca tccgcctcgg ccaatccgcc
     8401 accaccctct ccggcggcga agcccagcgc gtcaaactcg ccttggaact ctccaaacgc
     8461 gacaccggca gaacgctcta catcctcgac gaacccacca ccggcctgca cttcgccgac
     8521 atcgccctgc tgctggaagt cataggccgt ctgaaaggca aaggcaactc gattgtgatt
     8581 atcgagcata atctggatgt aattaaaacc gcggattgga ttgtggactt agggccggaa
     8641 ggcggcgatg gtgggggtaa ggttatcgcg aaaggtagcc ctgaacaagt ggctaaaatt
     8701 aaggggagtt ataccgggaa gtatttgaaa gtggttttga agtaaacgaa cggatataga
     8761 aaatgaacaa taattttgac cgcagcaagc atatctatca taacgaagct tttgtagaac
     8821 tggttaaaga tgccatacgc ttttttcatg gtacgcctgt tcatacactt cctccaccca
     8881 aacagttcaa aggtgcaggg gtttatgcga tttactatat cggtaacaat cctttgtaca
     8941 aacaatatgc ggactggaac agattatcct acaatgcgcc aatttatgtt ggcaaggccg
     9001 tccccaaagg ctggcggcaa gcaagaaatt ctgataatgc gctaaaccaa tcaaccgaac
     9061 tgttccaccg gttgaaagaa cacagcagga gtattgccgc tgtttctgat ttagatcctt
     9121 ccgatttcat gtgcagattc gttatctttg aaggtgccgg ttctgacatg ataggcacaa
     9181 tcgaagctgc cctgattaaa ttacacaaac ctctatggaa ctcttgtgtc gatggtttcg
     9241 gaaaccacga ccccggaaaa ggccgttacg aacaagccaa atcggattgg gatgtccttc
     9301 atagcgggcg ggtttgggca gataggctaa acggcatacc gaatagctat gagtccatac
     9361 ttgaaaatat caacacacat ttagagataa taaagagaaa ataacctatg aagtctttgg
     9421 aaattttttc cggcgcgggc gggttggcaa aaggccttga acttgcagga ttccaacatg
     9481 catcttttat tgaactcaat aaagatgcat gcaattcttt aagaagcaac tttaacccca
     9541 agcttgttta tcagggagat gtggcagatt ttgatctttc cagtcaagaa ggcatagaag
     9601 tcattgcagg cggtcctcct tgccagccct tttcattggg cgggaaacac cttgcccatg
     9661 aagacagaag ggatatgttt cctcatgcgg tcagatatgt ggagtattac cgccccaaag
     9721 cttttatctt tgagaatgta aaaggacttt taagaaaatc attcgctgat tattttgaat
     9781 atatcctact tcggcttact tatccgaatc ttggtatttt gcagaatgaa gattggaaag
     9841 gccatctgac acggctgaaa gaaattgaat tcaatttata taaaggaatc aaatataagg
     9901 tttcatatca attgttgaat gcagctgatt acggagtgcc gcaaaaacgc gaacgcgtgg
     9961 ttatcgtagg tatccgggcc gatttggata ttgattggaa attcccaaaa agaacacatt
    10021 ccgaagacag gttgaattgg gaaaagtatg taacggggga atactgggaa aaacacaacg
    10081 aacccaaaag attcaataaa gatattgctg aaaagttaca aaaaaaatac ggtatattcg
    10141 aaccagaaaa aaaaccttgg caaacggtaa gggatacctt gtccgacatc ccgcatcctt
    10201 tggggaatca taaaattaca ggacatgaat atagggatgg cgcaagaatt tatcccggac
    10261 acacaggaag cgggatagac gaaccgtcca aaaccattaa agcaggtggg catggcgttc
    10321 ccggcggaga aaatatgatt cgttatgatg atggaacagt cagatacttt accagctatg
    10381 aagcaaaact tcttcaaaca ttccctgaag aatttgtcat ttctggagct tggggagaag
    10441 caatgcgaca aattggcaat gcggttcctg tcaaattgtc ggaaatttta ggcaaacatc
    10501 tgatgggggt gttgtcggag aaaagcagcc tgcacaacta acccgcccac tgcgtgtgtg
    10561 cggtacgcac gcggttgctt gattgacggt ttgccgtttc aacttttaac ttcgttgaag
    10621 ctagtgcttt cagacggcct ccaatgtcgt ttggaaacat caggtccaac aaagttaaac
    10681 cttaaacaac aaaccattcc ttctccaaca cccaaaccac aaacaaaaat cctatggcgc
    10741 gttatcgcag aaacttcatt gccggcggca cattcttttt caccgtcaaa cccgccgccc
    10801 cgaaatcgcg tctgcttgtc ggacatatcg gcttcgcgtg cggcttatat ggatgtgcaa
    10861 aaacaatatc cctttgaaac cgtcgccgta tgcgtgctgc cgaaccatat tcacgccatt
    10921 tggacgctgc cgcccgacga tgcggattat tccctactcc ggcggctgat taaaaccaaa
    10981 ttctccgcat attcccctca tactaaaaa
//
LOCUS       DAWTCI010000067        10918 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWTCI010000067 DAWTCI010000000
VERSION     DAWTCI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.067"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..464
                     /locus_tag="WM481_001660"
     CDS             <1..464
                     /locus_tag="WM481_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9080934.1"
                     /translation="VNILPFSKVKGLVKAAKLGRAAKPGRAAKLGKAKVSEDFAVASQ
                     ARYTSQTAENIIAADRIGSGLKADTSHRAASFLSQEQLGAGRTFSITGGDGIQRTLLQ
                     TKGSLNGQSGIYEYILDSNNKVTHQRFIKGGEITGQPNQRVKTKVRNRGKK"
     gene            461..934
                     /locus_tag="WM481_001661"
     CDS             461..934
                     /locus_tag="WM481_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFA9080935.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYENKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNIFELKPVLEDLASEMRDYSPKNWLYILLNDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSNHSYEENISRLYFNWGKYLSNKSRSG"
     gene            1063..1824
                     /locus_tag="WM481_001662"
     CDS             1063..1824
                     /locus_tag="WM481_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9080936.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGIRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPTKLKPTERYNRQTHYGGSQTD
                     GARAQAARQAGEGKPCPTCGRIQIFGTKTAPSPQHEPPLVKHYYEHGGHSMSNADRAK
                     HARESIKGTQCLTCQRKEGAMMSRYSREQAKKHGL"
     gene            1814..2362
                     /locus_tag="WM481_001663"
     CDS             1814..2362
                     /locus_tag="WM481_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFA9080937.1"
                     /translation="MDYNQTVLSHLQKFWKHHDIKGFTWTLGRIVEELPDFQVFQVIP
                     NHEDEPWVYVSSGIGQFLGQEFFIISPFETPEHIETLAMLASASMHYPDQFQLGKTVN
                     IGRPWVEQSSFRHFLISLPYPYGQELEYMDNVRFFWLLPITQTERLFLNTHSVEELET
                     KFDEAGIDYLDINRASTVWQAG"
     gene            2659..3012
                     /locus_tag="WM481_001664"
     CDS             2659..3012
                     /locus_tag="WM481_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080938.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQTGLYPINGL
                     RHRPKGDTNGWYIWAGENFSYDKNFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            3002..3325
                     /locus_tag="WM481_001665"
     CDS             3002..3325
                     /locus_tag="WM481_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFA9080939.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQDEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            3384..3611
                     /locus_tag="WM481_001666"
     CDS             3384..3611
                     /locus_tag="WM481_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080940.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDGQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            3738..4281
                     /locus_tag="WM481_001667"
                     /pseudo
     CDS             3738..4281
                     /locus_tag="WM481_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
     gene            4416..5147
                     /locus_tag="WM481_001668"
     CDS             4416..5147
                     /locus_tag="WM481_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9080941.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYR
                     ETVYIKVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYGKGNQRYKDAPYHGKNDNSV
                     KSRAPTNGQAVLDNSVQVKSTSSQRVGVDKTNNEIVVLNQTRIFNDGSAEYHGHVRNW
                     KNLHTDQQNALKKAGLVNSKGKIKK"
     gene            5144..5479
                     /locus_tag="WM481_001669"
     CDS             5144..5479
                     /locus_tag="WM481_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080942.1"
                     /translation="MMADKEQVEKMIYSENKDCVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLRSKQGNPDFQGFAEDALDDI
                     SFFIFKKRP"
     gene            5527..6054
                     /locus_tag="WM481_001670"
                     /pseudo
     CDS             5527..6054
                     /locus_tag="WM481_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676498.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
     gene            6060..6458
                     /locus_tag="WM481_001671"
     CDS             6060..6458
                     /locus_tag="WM481_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9080943.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDAEDWKFFYFPIQHSKGRLR"
     gene            6489..6866
                     /locus_tag="WM481_001672"
     CDS             6489..6866
                     /locus_tag="WM481_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HFA9080944.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVQFDYYNKK"
     gene            6997..7734
                     /locus_tag="WM481_001673"
     CDS             6997..7734
                     /locus_tag="WM481_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFA9080945.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGNRLFAESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHTYFVKGDKAETEGVWVHNDCPYGGSNNLEKAKLRAERLSKND
                     RAGKDFTKAGKEAVIDLNRIQNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVI
                     PKSKGGQGTPKNGQVLCRGCNIKKSNK"
     gene            7731..8150
                     /locus_tag="WM481_001674"
     CDS             7731..8150
                     /locus_tag="WM481_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HFA9080946.1"
                     /translation="MKSKLTVVYYDLESNIAEEILSGNIMPDGNFLIQEIPLFAPNLA
                     LNDIVAIEREDKMLFFDHLIKASGNTTINIVVLDHFPKDLLAAIEEHSGKIRKNGENY
                     LSVNFPPKKYNSDLKGILNRYEEANILSYREACLGFS"
     gene            8317..9048
                     /locus_tag="WM481_001675"
     CDS             8317..9048
                     /locus_tag="WM481_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9080947.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGCKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGSQAETEGVWVHNDCPYGNLSDNKSVGEGKKFTPAQK
                     KAIIQENMNRNGGVVKSDQSGEVLVRPKKSQKGITPPHQMNGRLTMCKQKVKEVQIVI
                     KMHRFYPEEKILRNQINRYQYAMEI"
     gene            9032..9304
                     /locus_tag="WM481_001676"
     CDS             9032..9304
                     /locus_tag="WM481_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080948.1"
                     /translation="MQWKFEDPQNTAVITTRRILSKKNGILEVWHDADDGMWQFLDGN
                     EIHEGDAVIVSLKEIVTIDSSVNLLSNLPLGGYAWRNTISEKWNFD"
     gene            9471..9701
                     /locus_tag="WM481_001677"
     CDS             9471..9701
                     /locus_tag="WM481_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFA9080949.1"
                     /translation="METLDERIKNLGKSLEDRIDANLIDATLEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            9850..10479
                     /locus_tag="WM481_001678"
     CDS             9850..10479
                     /locus_tag="WM481_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFA9080950.1"
                     /translation="MVKTADGYKAIARIRAGESVLSKDEASGVTGCKPVTARYGNPYR
                     ETVYIEVSDGIGNSQTLISNRIHSFYSGGKWIKAEDLKAGSRLLSESGRTQTVRKTVV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESKIAELTNKIIPGMSKNERLKIKQKIKNIAKNANRKTKGEEHGRRGR"
     gene            10460..10915
                     /locus_tag="WM481_001679"
     CDS             10460..10915
                     /locus_tag="WM481_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080951.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
BASE COUNT         3646 a         2039 c         2336 g         2897 t
ORIGIN      
        1 tggtcaacat cctgccgttt tccaaagtca aaggattggt gaaggcggcg aaactgggga
       61 gggcggcaaa accggggagg gctgcgaaac tagggaaggc aaaggttagc gaggattttg
      121 ctgttgccag tcaggcaaga tatacttcgc aaacggcaga aaatataatt gcagccgata
      181 gaataggaag tggattaaaa gctgatacgt cacatcgtgc agccagcttt ctatctcaag
      241 aacaattggg agcaggaaga acattctcaa ttactggcgg cgatggtatt caaagaactt
      301 tattgcagac aaaaggttct ttgaacggtc aaagcggtat atatgaatat atcctcgatt
      361 caaataacaa agtgactcat caaagattca ttaaaggagg tgaaattaca ggacaaccaa
      421 accaacgcgt gaaaactaaa gtacgcaacc gggggaaaaa atgaaccttg atttaaccgc
      481 tcaaaaagtc cgcctttctt ggaaggatat tctgtggggg tatgagaata aatacttggg
      541 ttgggctgat gtggcagctt atgcccgaaa aatgacgctt tcagatcatg atgaacgcgt
      601 gttcaagcta tctttaatca acaaatccaa tatttttgaa ttaaagcctg ttctagaaga
      661 tttggcttcg gaaatgaggg attattcccc taaaaattgg ctgtacatcc tcctaaatga
      721 cgtattccat agaaaagaag aatttgagga tcctctgggg gaagttgaaa aaatttatgc
      781 ggattttgat tacccggaag aaatagaatc atttgtcagg tatatgccgc ccaaagacgg
      841 ctatatccct tctaatcata gttatgaaga aaatatttct cggttatatt ttaattgggg
      901 aaaatatttg agcaacaaga gtaggtcggg ttaaaaattc aacaatcaaa aggtcgtctg
      961 aaactgcttt taattttcag gcgaccttcc ccttatttta aacaggatat tgggaactaa
     1021 gttcttcaaa aatcctacac ctgctccttc cacggcagca ccttggtcaa aacggcagac
     1081 ggctacaaag ccattgcccg tatccgagcc ggcgagagcg tcctctccaa ggacgaggca
     1141 agcggagaaa cgggatacaa acccgttacc gcccgatacg gtaatccgta ccaagaaacc
     1201 gtttacattg aagtttcaga cggcatcggc aacagccaaa ccctgatttc caaccgcatc
     1261 catccgtttt attcggacgg caaatggatt aaagcggaag atttaaaggc gggaatcagg
     1321 ctgttatccg aaagcggcaa aacccaaacc gtccgcaaca tcgttgtcaa accaaaaccg
     1381 ctcaaagcct acaatctgac cgttgccgat tggcatacct actttgtcaa aggcgataag
     1441 gcggaaacgg aaggggtttg ggttcataat gattgtccaa ccaaactaaa accgacagaa
     1501 cgttataatc gtcaaacgca ttatggaggt tctcaaacag atggagcaag ggctcaggca
     1561 gcaagacagg caggagaagg taaaccttgt ccaacttgtg gtagaattca gatatttggt
     1621 acgaaaaccg caccaagtcc gcaacatgaa cccccattag taaaacatta ctatgaacat
     1681 ggtggtcact ctatgagcaa tgctgatcga gcgaaacatg ccagggaaag cataaaagga
     1741 actcaatgtt taacatgtca aaggaaagag ggtgcgatga tgtcaagata ttcgagagaa
     1801 caggctaaaa agcatggact ataaccaaac tgttttatct cacttgcaaa aattttggaa
     1861 acatcatgat attaaaggat ttacttggac tttaggaaga attgttgaag aacttcccga
     1921 tttccaagtt ttccaagtga ttcctaatca tgaagatgag ccttgggttt atgtcagctc
     1981 tgggataggt caatttttag ggcaggagtt ttttatcatc agcccgtttg aaacacctga
     2041 acatattgag accttggcaa tgctggcatc tgcaagtatg cattatcctg atcaatttca
     2101 attagggaaa accgtcaata tcggtagacc gtgggtcgag caatcatcct tccggcattt
     2161 tctgatttct ctgccttatc cttatgggca agagttggaa tatatggata atgtccgatt
     2221 tttctggtta ttgccaatta cccagacaga aagacttttc ttgaatactc attcggtaga
     2281 agaattggaa acgaaatttg atgaagcggg tattgattat ttagatataa atcgtgcgag
     2341 tactgtctgg caggcaggat aaaaattcgg caatcaaaat gccgtctgaa acaagtttgg
     2401 actttcagac ggtattctga tgaatggaac aggatactgg gaactgagtt cttcaaaaat
     2461 cctatctccg tcccttatac gtcggtgctt tagtcaaaac ggcagacgga tgaaaaatta
     2521 ttgcccgtat ccttgccaaa gatgaagcaa gatgaagcca ggtaattgaa tcggtacaag
     2581 cgcaatgtcc gacttgttct gcaaaacagg gagggagatt aagccaatat tcaaaagaac
     2641 agaaaaagaa actgagtaat gggaataaaa aatgaacaaa taataatttg taaaaaatat
     2701 aatacagaaa tatatcctgt ttccgatgtt tcaaaaatcg gtgttgctga aaatgtcaaa
     2761 cagacaggcc tgtatccgat taacgggtta aggcaccgtc ccaaaggtga tactaacggt
     2821 tggtatatct gggccggtga aaacttctcc tatgataaaa attttttcct gcccttgcat
     2881 acgttccatt tgcaaatatg gcgacccgaa atcatcccct ttttgacact tccgcccggt
     2941 taccgttttt taattgggga aaacggttat gaagacgtat ggtttgatga attgctatta
     3001 aatgataatt gacgaaaaat ctacttcaga tgtactgaaa caatatcaag aactagggtt
     3061 tgatttgaca aaacctatga taatagagtt ttttatagga ggttctcaaa aaaatctgca
     3121 atcaatagaa agccaactca tttcttatcg gcaagatttt caaatcagta ttgaacaaga
     3181 tgaattcgga gaaatgtgga cttgctattg ttccgtcaat atcgtccctt cccttgaaaa
     3241 cattttagct attgaaacta cgttatttgg tattgcacaa aaaaatgatt gtaattatga
     3301 aggttttggt tcgtatggga actaacaaaa agtgtaggtt gggttgaaaa aaccatattc
     3361 ccattaaaga ttagggtgtg tctatggaca taaaattcaa tactttgggg gttatcctga
     3421 atggggtcaa tcctgaagaa aaattcataa aaattattga tggccaggaa aataccggtg
     3481 gatttctgat actgttatct tcaaatgaca aattctcatc atttgacagt tacgatgact
     3541 gggtcgaaaa cttagaaata ttaaaagaat acctgcaaga atctcattgg ataataaagt
     3601 gggtcggata gtcggcaaac gtagtccgtt tgaaacgctt ttacttttca gacggacttt
     3661 ctaccaatgg gaacaaggcg ttttgtctca atccggttta tgattattcg atacagtgat
     3721 gggaagctaa gaaaaatatg attaatcata ttgaaaaata tataggcact atttctcatg
     3781 gctcgaaaag cgattcggga agtcagtaca aattaaatat cgcagctatt ccctcttccc
     3841 ctaacaggga tttaaaaact tatattactt tagggttaag caaacatgat ctgaattata
     3901 aaagcagatt tgaaatactt tttgtatgtt ctttaaaata tgatgaaaat cagattttcc
     3961 catttttaag atggttagca gaaaccatca ttgagaataa aaaaaatcct tcttcggggg
     4021 caggttgttt atttgcctag aagcattgtt aattcaacaa aaatggacgc actgtatgtt
     4081 tccgccccat tttatttcga cgatgatttc caagtctgtt atggcgaaca ctacaatatt
     4141 gttttccctt tgcttgtccc cttgtataaa caggaagccg aattggtgga aaaaaagggt
     4201 tggaatgctt ttgagcagtt cttgttggat aatgaagttg gcaacctttc ggatatgaat
     4261 aggaagccgt ttgtttggta agggctttct taaatttgat taaaggtcgt ctgaaaccgt
     4321 gtttaatttt cagacggcat ttccttcatt tgaaacagga tattgaaaac taagttcttc
     4381 aaaaatccta cacctgctcc ttccacggca gcaccttggt caaaacggca gacggctaca
     4441 aagccatcgc ccatattcaa gccggagacc gcgtcctttc caaggacgag gcaagcggag
     4501 aaacgggata caaacccgtt accgcccgat acggtaatcc gtatcgggaa accgtttaca
     4561 ttaaagtttc agacggcatc ggtaacagcc aaaccctgat ttccaaccgc atccactcgt
     4621 tttattcggg cggcaaatgg attaaggcgg aagatttaaa agcgggaagc agactgttat
     4681 ccgaaagcgg cagaacccaa accgtccgca aaaccgttgt caaaccaaaa ccgctcaaag
     4741 cctacaatct gaccgttgcc gactggcata cctacttcgt caagggcagt caggcggaaa
     4801 cggaaggggt ttgggttcat aatgattgtc cgtatggaaa aggcaaccaa cgatataaag
     4861 atgctcctta tcatggtaaa aatgataatt ctgtgaaaag tagagcgcca acaaatgggc
     4921 aagctgtcct tgataactct gttcaagtta aatcgacatc ttctcaaaga gttggagttg
     4981 ataaaaccaa taatgaaatc gttgtattaa accaaactag aatttttaat gacggttctg
     5041 ctgagtatca tgggcatgta aggaactgga aaaatttaca tactgatcaa caaaatgctt
     5101 taaagaaagc aggattggtt aattcaaaag gaaaaatcaa aaaatgatgg ctgataagga
     5161 acaagtagaa aagatgattt attctgaaaa taaagattgt gttattgatg gtatgctcgg
     5221 tatgaccttc agctcggagg aggatgaaat cccttggatt tccgagaaat tgacggagtt
     5281 gtcgaagcat aaagacttgg atattgccag attgtcatta acctgtttcg gacatctcgc
     5341 caggatgcat gaaaatatcg gcgattgcga taaagtgatt gctttattgc gctcaaaaca
     5401 aggtaatccg gattttcaag gttttgccga agatgcgtta gacgatattt ccttctttat
     5461 atttaaaaag cgtccgtaag cgcggattaa acccggaaat ccgatgcgtc tgaaagtaca
     5521 ttccggcttt cagacggcat cggtaacagc caaaccctga tttccaaccg catccatccg
     5581 ttttattcgg acggcaaatg gattaaagcg gaagatttga aagcgggaag caggctgttt
     5641 gctgaaaacg gtgcagagca gaccgttcaa agcgttaccg tcaaaccgga accgctcaaa
     5701 gcctacaatc tgaccgtcgc cgactggcat acttatttcg tcaagggtag tcaggcggaa
     5761 acggaagggg tttgggttca taatgcgtgt ccgccaagaa aaacaccatc tacccccgta
     5821 tatgggaatg attcagaagc atatgcagca gcaaagaaat tgggatatag aaaaataaaa
     5881 gaaagaacga gaaatgatgc tgctattttt aaaaaaggaa agagttatat atcacgcgat
     5941 gtagattcac ataatggagg ggcttggaaa gaagcatctt cacccaaaaa tctgaacaga
     6001 aaagaaacaa gaaacggtac atttgataaa aacctaaatc gtattggaga ttaattatta
     6061 tgtgcgagtt caaggatttt agaagaaaca tcccttgttt tgaagagtat gacgaaaatt
     6121 catttattgg caaatggtat gatgacggag tgtgggatga tgaagaatat tggaaattgg
     6181 agaatgcttt aatcgaggtt agaaaaaaat atccttatcc gatggatata ccaagggaca
     6241 tcgtgattgg aatcggtacc attattgatt ttttaatggt tcaaaattgg aaactttttg
     6301 aaattaaagc ttctccttgg ttgcctaaaa gtgtcaaaat taatgagcgt tatgaaaggt
     6361 tcagagtaat gctccgttat atttttactg atctagatgc agaggactgg aaattttttt
     6421 actttccaat acaacatagt aaaggtagat tgagatgaaa agtttaatta tattggagat
     6481 acattattat gtgtgagttc aaggatatta taagaaacgt tccttatttt gaggggtatg
     6541 acgaaaattc atttattggc aaatggtatg atgacggggt gtgggatgat gaagaatatt
     6601 ggaagttgga gaatgattta atcgaggtta ggagaaaata tccttatccg atggatatac
     6661 caagggatat cgtgattgga atcggtacca ttattgagtt tttaatggtt ccaaattgga
     6721 aactttttga aattaaagct tccccttggt tacctgatag cgtgggaatt aacgaacgtt
     6781 atgaaaggct taaaacaatg ctccgttata tttttaccga gaaagacata gtcaacgtgc
     6841 aatttgatta ttacaacaaa aaatagcaaa acccggcaat caaaatgccg tctgaaaccg
     6901 tgtttaattt tcaggcgacc ttccccttat tttaaacagg atattgggaa ctaagttctt
     6961 caaaaatcct acacctgctc tttccacggc agcaccttgg tcagaacggc agacggctac
     7021 aaagccatcg cccatattca agccggagac cgcgtccttt ccaaggacga ggcaagcggg
     7081 aaaacaggat acaaacccgt taccgcccga tacggcaatc cgtatcaaga aaccgtttac
     7141 attaaagttt cagacggcat cggcaacagc caaaccctga tttccaaccg catccacccg
     7201 ttctattcgg acggcaaatg gattaaggcg gaagatttaa aagcgggaaa caggctgttt
     7261 gccgaaagcg gcaaaaccca aaccgtccgc aacatcgttg tcaaaccaaa accgctcaaa
     7321 gcctacaatc tgaccgttgc cgactggcat acctactttg tcaaaggcga taaggcggaa
     7381 acggaagggg tttgggttca taatgattgt ccgtatggcg gttcaaataa tttagagaaa
     7441 gcaaaacttc gcgcagaacg gcttagtaaa aacgatcggg caggaaaaga ctttactaaa
     7501 gcaggaaaag aagccgttat tgatttgaat aggattcaaa ataatggtca agttaagtgc
     7561 gctaattgcg gtattgaaac aattccagcc aaacaatcta taaaaaatat ttctcctact
     7621 tcaaatgaaa gacaagtaga tcatgtcatc cctaaatcta agggtggtca aggtacacct
     7681 aaaaacgggc aggtattatg cagaggctgc aacattaaga agagtaacaa atgaaatcga
     7741 aacttactgt cgtctattac gatttggaaa gcaatattgc agaagaaata ctgtcaggaa
     7801 acataatgcc tgatggaaat tttcttattc aagagattcc tcttttcgca ccgaatttgg
     7861 ctttaaacga cattgttgcc atagaacgtg aggataagat gctgtttttc gaccacttga
     7921 taaaagcttc aggaaatacc acgataaaca tcgttgtttt ggatcatttc ccaaaggatt
     7981 tattggcagc catagaagaa cacagtggta aaatcagaaa aaatggagag aattatttat
     8041 cggttaattt tccgcccaaa aaatataatt ctgatttaaa aggaatttta aatagatatg
     8101 aggaagcaaa tatcctcagc tacagggaag cttgtttggg cttctcttaa tcgatctata
     8161 agtaagaatg gattacgtta gattgtaaac ctaacgatta aaaggtcgtc tgaaaccgtg
     8221 tttaattttc agacgaccta tttccttcat ttgaaacagg atattgggaa ttaagttttt
     8281 caaaaatcct acacctgctc cttccacggc agcaccttgg tcaaaacggc agacggctac
     8341 aaagccattg cccgtatccg agccggcgag agcgtcctct ccaaggacga ggcaagcggg
     8401 gtaacgggat gcaaacctgt taccgcccga tacggcaatc cgtatcggga aaccgtttac
     8461 attgaagttt cagacggcat cggcaacagc caaaccctga tttccaaccg catccactcg
     8521 ttttattcgg gcggcaaatg gattaaggcg gaagatttaa aagcgggaag cagactgtta
     8581 tccgaaagcg gcagaaccca aaccgtccgc aaaaccgttg tcaaaccaaa accgctcaaa
     8641 gcctacaatc tgaccgttgc cgactggcat acctacttcg tcaagggcag tcaggcggaa
     8701 acggaagggg tttgggttca taatgattgt ccgtatggaa atttatcgga taataaatct
     8761 gttggcgaag gaaaaaaatt tacaccagct caaaagaaag caatcatcca agaaaacatg
     8821 aatagaaacg gaggggtagt gaagtctgac caatctggag aagttttggt tcgtcctaaa
     8881 aagagccaaa aaggtatcac ccccccccat caaatgaatg gcagattgac catgtgcaag
     8941 caaaaagtaa aggaggttca aatagttata aaaatgcaca ggttttatcc agaagagaaa
     9001 atattaagaa atcaaataaa taggtatcaa tatgcaatgg aaatttgaag atcctcaaaa
     9061 tactgctgtt attaccacca gaagaatttt gtctaaaaaa aacggaatct tagaagtatg
     9121 gcatgatgct gatgatggaa tgtggcaatt cttagatggc aatgaaattc atgaaggaga
     9181 tgctgtgata gttagtttaa aagaaattgt cactattgat tcctcagtca atctcttaag
     9241 taatttacct cttggtggtt acgcttggag aaatactata tcggaaaaat ggaattttga
     9301 ttagcaagcg taggttaggt taaaaaaacc aacaatcaaa ggtcgtctga aaccgtgttt
     9361 agtttttcaa acgacctttt cttcaattaa catagaatgt tgggtctcga cccaacctac
     9421 acgcttacta atctcaaata taaccttatg aaataatgag tatttaaaag atggaaacat
     9481 tagacgaacg aataaagaat ttaggaaaat ctcttgaaga tagaattgac gctaatctaa
     9541 ttgatgccac actagagtac attacttttt cagaacgttt gctagctttt gaaacgctat
     9601 gtgattatat tgaagatttc aatatccagc ttacggaaaa agaatcccaa gaaatctctt
     9661 ttattaataa ggaatttgga attgaaagta catcagatta ataaatatta ggtcggttta
     9721 aaaacccaac aatcacaagg tcttctgaaa ccgtgtttaa ttttcagacg gcatttcctt
     9781 catttgaaac aggatattgg gaattaagtt tttcaaaaat cctacacctg ctccttccac
     9841 ggcagcacct tggtcaaaac ggcagacggc tacaaagcca ttgcccgtat ccgagccggc
     9901 gagagcgtcc tctccaagga cgaggcaagc ggggtaacgg gatgcaaacc tgttaccgcc
     9961 cgatacggca atccgtatcg ggaaaccgtt tacattgaag tttcagacgg catcggcaac
    10021 agccaaaccc tgatttccaa ccgcatccac tcgttttatt cgggcggcaa atggattaag
    10081 gcggaagatt taaaagcggg aagcagactg ttatccgaaa gcggcagaac ccaaaccgtc
    10141 cgcaaaaccg ttgtcaaacc aaaaccgctc aaagcctaca atctgaccgt tgccgactgg
    10201 catacttact tcgtcaaggg taatcaggcg gaaacggaag gggtttgggt tcataattct
    10261 tgtccgccta aaagaacagg aagctccaag aatgaaaaac atggagacgg cggtcgaagt
    10321 caaatatcag cggaatcaaa aattgctgaa ttaacaaata aaattattcc cggaatgtcc
    10381 aaaaatgaac gcttaaagat taagcaaaaa atcaaaaata ttgccaaaaa tgccaatcga
    10441 aaaacaaaag gagaagagca tggtcgacgc ggtcgttaaa actcccgaat ttttaccttt
    10501 tacttctgta ggaatattcc gttttggtgc tgatataaca cagtacaaaa acatactgga
    10561 aacttttatg tatgaacctc ctgatgagtt tggaacagaa tattatgaat ctcccgactc
    10621 aaatttactc atttccgtca aaaaaaacaa aattatttcg atattttgtt atcaggagct
    10681 atattttatg ggcgttaata taataggtct gaattttgaa gattttaaac agcttttcca
    10741 tcaccctaaa tcatacggtg ttgataaata ttatttatca aatgaatctt atccgacata
    10801 tgtctatgag tttgatgaaa taggagtaca agcatgggaa gcaaaaggga aaattgttac
    10861 tatcattgcc gggggcaaag acaattattc gacagaacct tactacgatg aataacat
//
LOCUS       DAWTCI010000068        10915 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWTCI010000068 DAWTCI010000000
VERSION     DAWTCI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.068"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            200..709
                     /locus_tag="WM481_001680"
                     /pseudo
     CDS             200..709
                     /locus_tag="WM481_001680"
                     /inference="COORDINATES: protein motif:HMM:NF014500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
     gene            684..1586
                     /locus_tag="WM481_001681"
     CDS             684..1586
                     /locus_tag="WM481_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFA9080952.1"
                     /translation="MHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYML
                     GKVLGRAIHVPVPELLSGGTRDSDPPKEPAKAGTVVAVMLIPMLLIFLNTGVSALISE
                     KLVSADETWVQTAKMIGSTPVALLISVLAALLVLGRKRGESGSTLEKTVDGALAPVCS
                     VILITGAGGMFGGVLRASGIGKALADSMADLGIPVLLGCFLAALALRIAQGSATVALT
                     TAAALMAPAVAAAGFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLK
                     TWTANQTLIALIGFALSALLFAIV"
     gene            1606..2124
                     /locus_tag="WM481_001682"
     CDS             1606..2124
                     /locus_tag="WM481_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFA9080953.1"
                     /translation="MTTHFVVTGVCGCGKTTAAPSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGADHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(2310..3227)
                     /gene="thrB"
                     /locus_tag="WM481_001683"
     CDS             complement(2310..3227)
                     /gene="thrB"
                     /locus_tag="WM481_001683"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFA9080954.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELSFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAGEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(3264..3992)
                     /gene="ubiG"
                     /locus_tag="WM481_001684"
     CDS             complement(3264..3992)
                     /gene="ubiG"
                     /locus_tag="WM481_001684"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFA9080955.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            4144..5385
                     /locus_tag="WM481_001685"
     CDS             4144..5385
                     /locus_tag="WM481_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFA9080956.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGSAVGGKISLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTF
                     IWATGGLVADAKPSVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWAGTLVALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSMSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGCVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIANIAAQVLSQMELVPVFKG"
     gene            5537..6592
                     /locus_tag="WM481_001686"
     CDS             5537..6592
                     /locus_tag="WM481_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFA9080957.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQRRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIVAHSHMMRRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEQTEL
                     PVKLAVAGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESALCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDMLASV"
     gene            complement(6659..8311)
                     /locus_tag="WM481_001687"
     CDS             complement(6659..8311)
                     /locus_tag="WM481_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFA9080958.1"
                     /translation="MVAYAFLFLFVTAVVLLIVRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGMPWRWAGAVLFAAAFVSVRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETQPYGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRELVGNYRNYLYDVLKIAR"
     gene            8454..9017
                     /gene="gmhB"
                     /locus_tag="WM481_001688"
     CDS             8454..9017
                     /gene="gmhB"
                     /locus_tag="WM481_001688"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFA9080959.1"
                     /translation="MKLIILDRDGVINRDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQDLTEMHAKMHRLIRQAGGEIDGIWFCPHTGADGCNCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PGHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            9047..9790
                     /locus_tag="WM481_001689"
     CDS             9047..9790
                     /locus_tag="WM481_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFA9080960.1"
                     /translation="MLIIRNLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGI
                     LNWSLKHIVGLKYRIIGAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAEMPSETA"
     gene            9787..10476
                     /locus_tag="WM481_001690"
     CDS             9787..10476
                     /locus_tag="WM481_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFA9080961.1"
                     /translation="MTSLIHTLSDGIELTVEINRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPHNTANRLPEHIWFHGRQLALTAHQDTQILLMPS
                     EILVPEGAPEKQLALLRDFLERQAHSYLIPRLEHHARATQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPEAKQRI
                     KIHGRELFALG"
     gene            complement(10540..10731)
                     /locus_tag="WM481_001691"
     CDS             complement(10540..10731)
                     /locus_tag="WM481_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080962.1"
                     /translation="MPSENLSDGIFAGKSVEDFAPAQAFHTARRIGRVSEGRCEMKRC
                     GRRGCRHPAPNRRQSVIAV"
BASE COUNT         2540 a         3506 c         2683 g         2186 t
ORIGIN      
        1 agcgccttag ggaaccgttc cctttgagcc ggggcggggc aacgccgtac cggtttttgt
       61 taatccacta taaatcgttc aaataaacag gaatataact tcagacaaac aacttaccgc
      121 ccgatttgtg ctatcgtttt cgcacaactt aaaaaaacct gacaattttg tacttttatt
      181 acagagaaag gctttacaaa tggacggccg gacacagacg ctgtccgcgc aaaccttgtt
      241 gggcatttcg gcggcggcaa tcatcctcat tctgatttta atcgtcaaat tccgcatccg
      301 cgcgctgctg acactggtca tcgccagcct gctgacggct ttggcaaccg gtttgcccac
      361 aggcagcatc gtcaacgacg tactggtcaa aaacttcggc ggcacgctcg gcggcgtggc
      421 gcttctggtc ggtctgggcg caatgctcgg acgtttggta gaaacatccg gcggcgcaca
      481 gtcgctggcg gacgcgctga tccggatgtt cggcgaaaaa cgcgcaccgt tcgcgccggg
      541 cgttgcctcg ctgattttcg gcttcccgat tttcttcgat gccggactaa tcgtcatgct
      601 gcccatcgta ttcgccaccg cacggcgcat gaaacaggac gtactgccct tcgcgcttgc
      661 ctccgtcggc gcatttatcc gtcatgcacg tcttcctgcc gccccatccg ggcccgattg
      721 ccgcttccga attttacggc gcgaacatcg gccaggtttt gattttgggt ctgccgaccg
      781 ccttcatcac atggtatttc agcggctata tgctcggcaa agtgttgggg cgcgccatcc
      841 atgttcccgt tcccgaactg ctcagcggcg gcacgcgaga cagcgacccg ccgaaagaac
      901 ctgccaaagc aggaacggtc gtcgccgtca tgctgattcc catgctgctg attttcctga
      961 ataccggcgt atcggccctc atcagcgaaa aactcgtgag cgcggacgaa acctgggttc
     1021 agacggcaaa aatgatcggt tcgacacctg tcgcccttct gatttccgta ttggccgcac
     1081 tgttggtctt gggacgcaaa cgcggcgaaa gcggcagcac gttggaaaaa accgtggacg
     1141 gcgcactcgc ccccgtctgt tccgtgattc tgattaccgg cgcgggcggt atgttcggcg
     1201 gcgttttgcg cgcttccggc atcggcaagg cactcgccga cagcatggcg gatttgggca
     1261 ttcccgtcct tttgggctgc ttccttgccg ccttggcact gcgtatcgcg caaggttcgg
     1321 caaccgtcgc cctgaccacc gccgccgcgc tgatggctcc tgccgttgcc gccgccggct
     1381 ttaccgactg gcagctcgcc tgtatcgtat tggcaacggc ggcaggttcg gtcggttgca
     1441 gccacttcaa cgactccggc ttctggctgg tcggccgcct cttggatatg gacgtaccga
     1501 ccacgctgaa aacctggacg gccaaccaaa ccctcatcgc actcatcggc tttgccttgt
     1561 ccgcactgct gtttgccatc gtctgacaga cggaaaggat agtaaatgac tacgcatttt
     1621 gtcgttacgg gcgtatgcgg ctgcggcaag accaccgccg cgccgtccct gcagaaacac
     1681 ctcggtcaat gtccctatgc cgaaggcgac gagttccaca cccaagccaa ccgcgacaag
     1741 atgggcgcgg gtattccgct gaccgatgaa gaccgctatc cgtggttggg caatctgcgc
     1801 gactggatga cgcaacaggc gcaaaacggc gcggaccaca ccatcgtaac ctgttccgcc
     1861 ctcaaacgcg gctaccgcga cattctgcgc ggagccgaag gcaaagccgc cttcatccac
     1921 ctcagtccgc cgcaagacat caacctcgaa cgcatgatgt cgcgcaaagg gcactacatg
     1981 aaagcaggga tgctcgattc gcaactggaa atcctcgagg aactgggcga aggcgaatac
     2041 ggggtcaaaa tcgccaaccc cggcacgccc gaagcggtcg aagccgatat tctgaactgg
     2101 gttgcctcgg aaaacctgct ttgaagcaat atgccgtctg aagcccggca caggatgggt
     2161 ttcagacggc ataaacatcg ggaacagaat gaattacatt gatttaaaat ggaaaatacc
     2221 cggctatcgt ctcattttcg ttttaatcag ccataaaaat gccgtccgaa accctttcag
     2281 acggcatttc tgtcaaacgc cggacgcact caatccaaac tcaacagcag gttgcggaac
     2341 gcgttcgggt ctttgataaa cgtcatctcg cccgcctgcg gaaaatgaaa atccaacagg
     2401 cgcgacaccc aaaaacggat gcagccggcg cgttgggcgg tcgggaaata cgccttttcc
     2461 ccggcactca aggggcgcac gccctcataa ccgccgataa acgccttttt caacgcctca
     2521 tccaacttat tgtccgccgt ccttgcccaa tcgttgaccg caatcgccaa gtcatacata
     2581 aaattgcccc ggcaggcata atagaaatcg atgaagcccg atacctgacc gccgtcaagc
     2641 aacacattgt ctttaaacag gtcggcgtgg atgatgcccg aaggcagatg gttgccgaga
     2701 ttgtccttca gcgcatcgat ttcggaacac agcagcgcgg catcgtcttg cgacaggacg
     2761 ggcagcagcc gggcgcacgc ttccgtccac cacgcatcgt aacgcgggtt ttccatttcc
     2821 aaagggaaat cggcggcggc aaggtgcatt ttcgccaaca tcgcaccggt atggaaacac
     2881 tgctcagccg tcggcagcgc ggtatccgaa cctttcaggc aggcaaccag gcaggccggc
     2941 ttgcccgcca aaacggaatc gagccggccg tctttgcgcg caaccggcgc ggcaaccgcc
     3001 acgcccttca tactcaaatg ccggttaagc tccagaaaaa acgacagctc ttcctgtttc
     3061 aacacttcaa acacggtcag cacataacgt cccgaagtcg tcgtcagaaa ataattgctg
     3121 ttggtaatcc cctgcgcaat gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc
     3181 aggaagccgc gcatttcatc atcggaaaca ctggtataga cagacatatc aacttacttg
     3241 ctcaaaaacg ggcaaacccg ccgtcagaac gccggacggc aggcaaacat ataattcaca
     3301 tcggtcgaat cgcacagggc ataagtttgc gacaacacat ggtaagtcat acccttcgta
     3361 tccgccacat ccaagcccgc ctgacggcac atccgcgcca gctcggcagg cgcgatgaat
     3421 tttttccagt cgtgcgtgcc tttggggacg aacttcaaca gatattccgc cgccacaatc
     3481 agatgcaggt acgatttcgg gtttttattg atggtggaaa aaaacaccat gccgtccggc
     3541 ttgaccagct tggcacaagc acgcacgatg gcggcgggat cggggacgtg ttccatcatt
     3601 tccatgcacg ttaccacatc gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg
     3661 atacattcgt attcgatatc ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc
     3721 agcgactgct ccgccatgtc gatgcccttg acaaacgccg cgccgcgccg cgccatgctt
     3781 tccgcaagga tgccgcctcc gcagcccacg tccaaaaccc gtttgccgca caaatccgcg
     3841 tgtccgtcga tataatcgag ccggagcgga ttgatgtcgt gcaaggtttt gaactcgccc
     3901 gacttgtccc accatttgtc ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca
     3961 ttatattttt tgtcggacat tttccctccc atctgacgaa ccgcccactc caaaacccaa
     4021 gattatacta tggaacacaa tgccgtctga aagccttttc gggcggcgcg gcgcaaatgc
     4081 cttacaaatc cttacacttt acggcataat ggcggctcgc tttttctggc agaaagacaa
     4141 aatatgccca ccaaaacccc ttcactgttc ggcggcgcga tgattatcgc cggcacggtc
     4201 atcggcgcag gtatgctcgc caacccgacc gccacatccg gcgtatggtt taccggctcg
     4261 ctggccgtgt tgctgtacac ctggttttct atgctttcca gcggcctgat gattttggaa
     4321 gtcaacaccc attatccgca cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga
     4381 cgcggctgga acatcatcaa cggcatcgcc gtcgccttcg ttttatacct gctcacctac
     4441 gcctacatct tcgtcggcgg cgacctgacc gccaaaggca tcggcagcgc agtaggcggc
     4501 aaaatttcgc tcaccgtcgg acaactcgtc ttcttcggca tcctcgcctt ttgcgtatgg
     4561 gcatccgcac gcttggtcga ccgctttacc ggcgtcctca tcggcggcat ggtattaacc
     4621 tttatttggg caaccggcgg cctggttgcc gatgccaaac cgtccgtcct cttcgacacc
     4681 caagcccccg ccggcaccgg ctactggatt tacgccgcca ccgccctgcc cgtctgcctc
     4741 gcttccttcg gcttccacgg caacgtttcc agcctgctca aatactttaa aggcgacgcg
     4801 cccaaagtgg cgaaatccat ctgggcaggt acgttggttg ccttggtaat ttacgtcctc
     4861 tggcaaaccg ccatccaagg caacctgccg cgcaacgagt tcgcccccgt gattgccgcc
     4921 gaagggcaag tctccgtcct gattgaaacc ctgtccaaat tcgcccaaac cggtaatatg
     4981 gataaaatat tgtccctatt ttcctacatg gcaatcgcca cctcgttttt aggcgtaacc
     5041 ttaggcctgt tcgactacat cgccgacatc ttcaaatgga acgacagcat gtccggccgc
     5101 accaaaaccg ccgcgctgac cttcctgccg cccctgattt cctgcctgct cttccccacc
     5161 ggcttcgtta ccgccatcgg ctgcgtcggc ctggcggcaa ccgtctggac aggcatcatc
     5221 cccgccatgc tgctctaccg ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt
     5281 tacggcggct tgtggctgat ggtttgggtc ttccttttcg gcatcgccaa catcgccgca
     5341 caggtattga gccaaatgga actcgtcccc gtatttaaag gataaaggca aaatgccgtt
     5401 tgaaacccgc cggcggcttc aggcggcatt gccgcaacaa acggcaaccg tattccggca
     5461 cacagcgcat taccctaccc cctcacgcac aaatcccgcc ccgacaggcg cgggacgcaa
     5521 ccataaagga acaatgatga agctcaaaat agacattgca accaacaact tcaaacacgg
     5581 cggcggcacg gaacgctaca cattggattt ggtaaagggt ctgaacagac aaaacatcac
     5641 accggccgtt tatgcgacga aatttgatca cggcattccc gaatacgcca tgatcgaacc
     5701 ccatcttgtc gatcaacgcc ggacgctgaa aaaactacgc tcgttcctct tttcaagccg
     5761 gctcgctcaa accagaaaaa acagtgccgc caaactgatt gcctgccacc acgccgatta
     5821 cgccgacctc ctcatctgcg gcggcacgca cttgggctac ctgcaccata tggcgcaaaa
     5881 accgaacctg ctcgaccgcc tcgccatacg ccgcaaccgc agcaactacg ccaccgccaa
     5941 actgattgtg gcgcattccc atatgatgcg gcgcgaactg gtcggactat acggcgttcc
     6001 ccccgaaaga atccaagtcg cccccccccc cgcagatacg gaacgcttct ttccacaacc
     6061 tagagaaact gccgccctgc gcgccaaata cggttttgcc gaccatgaaa ccgttttcct
     6121 attcccatcg accggccaca cgcgcaaagg cctggaactg ctcgccgact ttttcgaaca
     6181 aaccgaactg cccgtcaagc tcgccgttgc cggttcaccg cttccccgcc cgatgaaaaa
     6241 cgtcgtcgga ctgggcttct gcaccgatat gcccgaactc taccgcgccg ccgactttac
     6301 cattatggca tccctgtacg aacccttcgg gctggtcggt gtcgaatccg ccctatgcgg
     6361 cacacgcgtc gtcctctccg aaaacatggc atgtacagag gtcatgaacg aagaagccgg
     6421 attctttttc tcacgacaaa acccggaaac cctcgcgcaa gccgttgccc aagccgtcag
     6481 ccttaaaaaa cagggcggac accgcctgtc cgacccgatg cgggcgctga actacaaccc
     6541 gtccctgtcg caccacatcg accgactgac cgacatgctg gcatccgtat aacaaaatgc
     6601 cgtctgaacg ggttttcaga cggcattttc gcaaccgttg cagccgtgcg gcaaaaagct
     6661 aacgggcaat tttcaaaacg tcgtacagat agttgcggta atttccgacc aactcccggt
     6721 tcgggcagtc ttccaaccct ccgccgcaca aaccgcgcat cgccatattc gcacgcatga
     6781 agccgtcttt ggcttccaaa ccagccgcct ccatcagtac gccgcctgca aaggcaaggt
     6841 cgaggaaatc ctgccgctgt acgaatccgc cggcaatatt gctcctgacg gcaaactgcg
     6901 ttacataatc cggctgagcg taatcccatt tcttcctgac ggacacgccc tcaaacggta
     6961 cctgatgatc gccgaaataa ccgaaaacaa agggtttgcc gcgttcgtgc agataacggt
     7021 cgaaactttc caccgctttg tccaaatcgg caatgcgccc gatgtagtcg ttaagcgcgg
     7081 atacggtttt cgcattcaaa tcgggcgcat ccaaatcaaa cacattgtcg gtatccgtcc
     7141 gatacggccc gtgctccttc atggtcagca catatacgaa catcggctgc cgcacgtttt
     7201 ccaaatcggg atggcgtttt tcgagaatca tccgcgcata ctgcatcatt tcctcactgg
     7261 aaatgtgcca caggtttttg cccatcggcg cgggatagcc caaatcttgc ggctggaaca
     7321 tcagattaaa gccgaaatgg tcatatgctg ccttggcatt gtagttgccc ttggtaaacg
     7381 gcgagagcgc cacgcaaaaa taaccgtgtt cgcgcaggtt gcggacaaaa ccggtctgca
     7441 aatgcggtac gaccgaataa aacacgccgc ttgccaacgc gccgaaatct gtggacggaa
     7501 cacccgccaa aaacgcaaat tcggacttcc acgttgcgcc gccgaaagta tgcacgcgca
     7561 aaggtgagga aaatacagta tcttcctgcc gtccgaacat tttcaaatcg ggaattttgg
     7621 cggcggcaaa atcgaaacag tgcggatcga gcgtcgattc catcagggta acgacaatat
     7681 cgggcttttc atccgacatg ccgtacggct gggtttcaga cggcatctgc ctggcaaacg
     7741 cttccccgtc gccctcgaac acgggtactt ggaaaaacac cgcccgacag gacatcggca
     7801 ggttcaaaaa cacgtcgcgc ccgtcgtccg gcagcgagtc cagccacgtc tttaccgcgc
     7861 cggggtgctt ggaaaaatgc cgcaccgaca cgaacgccgc cgcaaacaga accgcgcccg
     7921 cccaacgcca cggcataccc aaagaatccg caccgctcca gccgaaaacc gcatatgccg
     7981 ccaaagccag cagccccgcc ataccgataa ccgcttcctt ataatgaaac agcgtttccc
     8041 aattccgcca gtcggcaatc agcaaaaagt cggaaatcaa cagcggctgt ttgtaataat
     8101 ggattttcag cctgtggaac agtatcagca ccacaaacca gaccgaagcg aaattcaagg
     8161 cgcgctgcca ctgcgccgtc aacatcagca taccgcccgc caaaaagaca aacagcgccg
     8221 acgcgaaaaa atacgtccag cggtagtgcg acctgacaat cagcagcacc accgccgtta
     8281 caaacaaaaa taggaaagca taggcaacca tcccgcccca ctcctcaaaa cggcatacct
     8341 gtgccgttgc gaattattat cgaaagcgtg cattataatg cagcacaggc gcatttccac
     8401 cgatatttca gtataatgcc atccccgaac ccgcccaacc caaaggaacc gtgatgaaac
     8461 tcatcattct cgaccgcgac ggcgtcatca accgggaccg cgacgacttc gtcaaatccg
     8521 ttgacgagtg gatacctgtc gaaggcagca tggatgcggt ggcattcctg acgcaggcag
     8581 gctgcaccgt tgccgtcgcc accaaccaat ccggcatcgg ccgcaaatat tttaccgttc
     8641 aagacctcac cgaaatgcat gccaaaatgc accgcctcat ccgtcaggcg ggcggggaaa
     8701 tcgacggcat ctggttctgc ccgcataccg gcgccgacgg ctgcaactgc cgcaagccca
     8761 aaccgggcat gattgaagac atcctcgaac gcttcaacgc ccaagcttcg gaaacttggc
     8821 tggtcggcga cagcctgcgc gatttgcagg caatcgatgc cgtcggcgga aaacccgcgc
     8881 tggttctgac cggaaaaggc aaaaaaacgc tctcccaaca cggacacgaa ttacccggac
     8941 acacacaggt tttcgatacc ctgctcgatt tctcacaata catcatgcag gaaaacgccg
     9001 caccgcaagc cgactgaaca taccgcattt ccgacaaggc aaaaccatgc tcatcatccg
     9061 caacctgatt tactggctga tactctgttc cagcctgatt ttcctctttc cctttatgct
     9121 gctcgcctcg cctttccggg acggggcgca caagatggcg cgggtctggg tcggcatcct
     9181 caactggtcg ctcaaacaca tcgtcgggct caaataccgc atcatcggcg cggaacacat
     9241 tccggaccgc ccctccgtca tctgcgccaa acaccaaagc ggctgggaaa cgctcgcgct
     9301 ccaagagatt tttccgccgc aggtttacgt tgccaagcgc gagttgttca aaatcccctt
     9361 tttcggctgg ggcttgaaac tggtcaaaac cataggcata gaccgcaaca accgccgcga
     9421 agccaacgaa cagctcataa aacagggttt ggcgcgcaaa aacgaaggtt attggattac
     9481 cattttcccc gaaggcacgc gccttgcgcc cggaaaacgc ggcaaataca aactcggcgg
     9541 cgcgcgcatg gcgaaaatgt ttgagatgga catcgtcccc gtcgccctca acagcggcga
     9601 attttggccg aaaaattcct ttctgaaata tccgggggaa atcaccgtca tcatctgtcc
     9661 gaccatcccg cacgcaagcg gcagcgaagc cgaattgatg gaaaaatgcg aacacctcat
     9721 cgaaacgcag cagccgctca tttccggcgc aggcccgttt gccgccgaaa tgccgtctga
     9781 aaccgcatga cctccctgat tcacaccctt tcagacggca tagaactgac cgtcgaaatc
     9841 aaccgccgtg ccaagaaaaa cctgattatc cgccctatcg gcacacatac cgtccgcatc
     9901 agcgtcccac cctgcttctc cgtctccgcc ctcaaccgct ggctgtatga aaacgaagcc
     9961 gtcctgcggc ggacactggc gaaaacaccg ccgcacaata cggcaaaccg actgcccgaa
    10021 catatctggt tccacggcag gcagcttgcc cttaccgccc atcaagacac gcaaatcctg
    10081 ctgatgccgt ctgaaatcct cgtccccgaa ggcgcgcccg aaaaacagct tgcgctgctg
    10141 cgggactttt tggaacggca ggcgcacagt tacctgattc cccgtctcga acaccacgcc
    10201 cgcgccacgc aactgttccc cgcctcctcc tcgctgacct ccgccaaaac cttctggggc
    10261 gtgtgccgca aaaccacagg catacgcctc aaccggcggc tggtcggcgc acccgaatac
    10321 gttgccgact atgtctgcat acacgaactc tgccacctcg cccatcccga ccacagcccc
    10381 gccttttggg aactgacccg ccgcttcgcc ccctacacgc ccgaagcaaa acagcggatc
    10441 aaaatccacg gcagggagct tttcgccttg ggctgacgca gccccgggcc gacgctttca
    10501 gacggcatcc gtgccggaac aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg
    10561 ccgccggttc ggggcaggat ggcggcatcc gcgccgtccg caccgtttca tttcacaccg
    10621 cccttccgaa acccgcccga tccgacgtgc ggtatgaaac gcttgagccg gcgcgaagtc
    10681 ttctactgat ttgcccgcga aaataccgtc cgaaaggttt tcggacggca tttttttgcg
    10741 ttttcctgga tggcggcgga ttcgtaaaag gcgggcaggg tggattgcag gatgggttga
    10801 ggcctgccaa atccgccaac tctaccgaat acgccgccgt cattcccgcg caggcgggaa
    10861 tttggttttg tcggtgcgga aacttatcgg ataaaacggt ttcttagatt ccacg
//
LOCUS       DAWTCI010000069        10805 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWTCI010000069 DAWTCI010000000
VERSION     DAWTCI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10805
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.069"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..188)
                     /locus_tag="WM481_001692"
     CDS             complement(<1..188)
                     /locus_tag="WM481_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080963.1"
                     /translation="MFPPAEITGRGRVVLQQGFAGFGLYFHGVKFRRLNIPFKLPASC
                     RAVKYARMFAAVSADFGG"
     gene            313..996
                     /locus_tag="WM481_001693"
     CDS             313..996
                     /locus_tag="WM481_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFA9080964.1"
                     /translation="MDKEKVLDKIKKCLALGRSANEHEAAQALRQAQALMEKYKVNAE
                     DIALSKVSEQKADRKMAFKLAGWQWGVANMIADIFGCKSYQRGKTMMFYGIGNRAETS
                     AYAFDVVYRQISADRRKFLKTCRAGKPSHRTYLADRFCGGWIASAWETVKKFEMSDEE
                     KAIMDGYKKKEYPDMAEARTRDAKSSILQGSKMEYEALTRGMESGKQVKLHYAVNGTG
                     FVKQIGGQT"
     gene            1191..1460
                     /locus_tag="WM481_001694"
     CDS             1191..1460
                     /locus_tag="WM481_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080965.1"
                     /translation="MESSDVQGRMETLAFATAPKKGGIRKSGFGRVILIKSGHIVIHN
                     ADGDSFATGYGVIAADIVSPDLKTDFDIFFPPESLLRYKKIGKKK"
     gene            complement(1816..2172)
                     /locus_tag="WM481_001695"
     CDS             complement(1816..2172)
                     /locus_tag="WM481_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080966.1"
                     /translation="MPLSRAMRWVLDQARGLNVNGRFVFESVNFQGHINKESPRVAMR
                     RSGLDTTAHGLRSLARTYLREVLKVYNDVAEKLLAHSLGTRTQTAYNRSELWEERKDA
                     PERWGNDVLRLADNGK"
     gene            complement(2121..3016)
                     /locus_tag="WM481_001696"
                     /pseudo
     CDS             complement(2121..3016)
                     /locus_tag="WM481_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(3207..3296)
                     /locus_tag="WM481_001697"
     tRNA            complement(3207..3296)
                     /locus_tag="WM481_001697"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3260..3262),aa:Ser,seq:cga)
     gene            complement(3373..3463)
                     /locus_tag="WM481_001698"
     tRNA            complement(3373..3463)
                     /locus_tag="WM481_001698"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3427..3429),aa:Ser,seq:tga)
     gene            complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="WM481_001699"
     CDS             complement(3546..4325)
                     /gene="yaaA"
                     /locus_tag="WM481_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFA9080967.1"
                     /translation="MFFVLSPAKNLNEKDPCPVSEFTQPDLLAESEILMRQLRELAPQ
                     QIAELMHVSDKIALLNAERNAAWHTPFTPENAKQAVFMFNGDVYEGMDANTLNTNQIQ
                     YLQNHVRLLSGLYGLMRPLDLMQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="WM481_001700"
     CDS             complement(4481..5668)
                     /gene="dapC"
                     /locus_tag="WM481_001700"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFA9080968.1"
                     /translation="MNTLLKQLKPYPFARLREAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLRELEKYPLTAGLPELRQACANWLKRRYDGLTVNPDNEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWERTKLVFVCSPNNPSGSVPDLDGWREVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQSGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVC
                     LYR"
     gene            complement(5740..6192)
                     /gene="dut"
                     /locus_tag="WM481_001701"
     CDS             complement(5740..6192)
                     /gene="dut"
                     /locus_tag="WM481_001701"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFA9080969.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYTAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     GSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGSH"
     gene            6358..7069
                     /locus_tag="WM481_001702"
                     /pseudo
     CDS             6358..7069
                     /locus_tag="WM481_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            7066..7374
                     /locus_tag="WM481_001703"
     CDS             7066..7374
                     /locus_tag="WM481_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HFA9080970.1"
                     /translation="MKDLLSFPSFLLFSCMLAVTCPTQLIGFFALRNRTLSRRAQTVM
                     ETAPGCVLISVIAPYFVSDKPHELIAIALTAFAACRFSMLFTVLIGVGSSGISGWLMA
                     "
     gene            complement(7444..7917)
                     /locus_tag="WM481_001704"
     CDS             complement(7444..7917)
                     /locus_tag="WM481_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFA9080971.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNVLKQFILKNPQDNNQIIKSKLETFVLGYKMNPKIAKKYSVSVAFANTEKPRA
                     YRLVGVPKAGTGYTLSVWMNSVGDGYKCRNATSAQTYSETLSANTGCEAFSNRKK"
     gene            complement(7919..8530)
                     /locus_tag="WM481_001705"
     CDS             complement(7919..8530)
                     /locus_tag="WM481_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFA9080972.1"
                     /translation="MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRT
                     NDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGKVSKMP
                     RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ"
     gene            complement(8509..9450)
                     /locus_tag="WM481_001706"
     CDS             complement(8509..9450)
                     /locus_tag="WM481_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFA9080973.1"
                     /translation="MKRKMLNVPKGGYDGMKGFTIVEFLVAGLLSVIVLIAVVSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGSFGCFNMSEHIGNDVVFNVAQKNAL
                     FSLKRNSTNSTNKLIPITESPNINYQNFFQVSSALIFQYGIDDVDASADTTVVSSCAA
                     ISKPGKQIPTLEDAKKELKIQNSDKEQNGNIARQRHVVNAYAVGKIAGEEGLFRFQLN
                     EKGEWGNPQLLAKKIKRMRVRYIYVSGCPEDEDAGKEEQFKYTDKFDSSVTPAGVEVL
                     LDSGSDAKIAASSDNIIYAYRINATIRGGNVCANRTL"
     gene            complement(9447..10055)
                     /gene="pilV"
                     /locus_tag="WM481_001707"
     CDS             complement(9447..10055)
                     /gene="pilV"
                     /locus_tag="WM481_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFA9080974.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYTGSYPPTSSDGDFKLNNL
                     ISKTDLAKAQLDRFGYELKQALPDAVAIRYAVCKDSSGDAPTLSGNTFSSNCDKKANG
                     DTLIKVLWVNDSAGDSDIFRTNLGVSGGNIVYTYQARVGGRE"
     gene            complement(10087..10749)
                     /locus_tag="WM481_001708"
     CDS             complement(10087..10749)
                     /locus_tag="WM481_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HFA9080975.1"
                     /translation="MCTRKQQGFTLIELLIVMAIAAIMATIALPNMGGWIASRRIASH
                     AEQVANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNGCDFSKKGRGMLAFGDKNGNK
                     AYDGDTKDVFLRNVVLNDTDDSRINYAFNHIAFGSSQPKADRVVWTFNQNGTFGYLPD
                     QNLKDNSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCRKNDTRAVC
                     KH"
BASE COUNT         2856 a         2819 c         2415 g         2715 t
ORIGIN      
        1 ttgccgccaa agtccgccga tactgcggcg aacatccggg cgtatttgac ggcgcggcag
       61 gaagcaggca gcttgaacgg tatattaagc cgtctgaatt tcacgccgtg gaaatacagc
      121 ccgaagcctg caaagccttg ttgcaaaact accctgccgc ggccggtaat ctcggcaggc
      181 ggaaacaaaa aaagccccga gttcgtaagc gtatcagccc tagccgaatg gcccgaccga
      241 atcgaaagca aagcgaaaga cgaatgggca agggtgcgaa attaaaatac cggacaaatt
      301 aaagggttta atatggataa agagaaagtc ttagacaaaa tcaagaaatg tttggctttg
      361 ggtcggtcgg caaatgaaca tgaagccgca caggcgttaa ggcaggcgca ggctttaatg
      421 gaaaagtaca aagtaaatgc cgaggatatc gccttatcaa aagtctccga acagaaagcc
      481 gatcggaaaa tggcttttaa attggcaggc tggcaatggg gcgttgcaaa tatgattgcc
      541 gatatattcg ggtgcaaatc ttaccaacgc gggaaaacaa tgatgtttta cggcattgga
      601 aatcgagcgg aaacctcagc ctatgccttt gatgtagtct atcgtcagat ttccgccgac
      661 cgccgcaaat ttttgaaaac ctgccgggcg ggaaagccct cgcacagaac ttatcttgcc
      721 gaccgatttt gcggcggttg gattgcgagc gcttgggaaa cagtcaaaaa atttgagatg
      781 tcagatgaag aaaaagccat tatggacgga tacaaaaaga aagaatatcc ggatatggcc
      841 gaagcaagga caagagatgc gaaatcgtca atcctacaag ggtcaaaaat ggaatatgag
      901 gcattaaccc gaggaatgga atcgggtaag caagtgaagt tacactatgc cgtgaacggt
      961 acgggcttcg taaaacaaat cggagggcaa acatgaatcg atacggcggc ggatgcggaa
     1021 acttcggcgg attggggaaa ggatatgccg aatatgaaag acgcaattgc cgcatgccaa
     1081 gccgtaaaaa agcggaaacc cggacaaatc ggaatcaacg gaaagaggat tgaagacatg
     1141 gaatacgaag tattcaataa acgcccgtca aaaaagcggt cggcgaagca atggagtctt
     1201 ccgacgtaca agggcggatg gaaacactgg catttgcaac cgccccgaaa aaaggcggaa
     1261 tccggaaatc cggattcggc cgcgttattc taatcaaaag cgggcatata gttattcata
     1321 acgctgatgg cgacagcttt gccaccggct acggcgtgat tgccgccgac atcgtcagtc
     1381 ccgatttgaa aacagacttt gacatattct tcccgccgga aagcttgttg cgatataaaa
     1441 aaatcgggaa gaagaaatag gcagacaaac gacagagccg taaaaacggc gcaagattaa
     1501 accgaacagg agagtaaaaa aatgaacatc accaaaccgg gcgcaaggat agaccgcccg
     1561 accatccgcg aactgattgc ctacgcaacc tgccgcaacc accccatttc aaactcgaca
     1621 ctgctgcgta tggaaaaaga cggacggata ccgtgccgtc tgaacctccc tcgcatcccc
     1681 cgtatgggac acccgcgaag ttttggaagc attagggttg cagcaataaa aaaacgcccg
     1741 attagggcgt tttcacataa tgcggaaaca gctttcttat ctacatttcc acataacgca
     1801 aaacacacaa aaaaatcatt ttccgttgtc ggcaagcctc aaaacatcat tcccccaccg
     1861 ttccggcgca tccttacgct cctcccaaag ctccgagcgg ttgtaagccg tttgcgtcct
     1921 cgtccccaac gaatgggcaa gcagcttttc cgccacatca ttatacacct tcagaacctc
     1981 gcgcaaataa gtacgcgcaa gcgagcgcaa accgtgcgcc gtcgtatcca gccccgaccg
     2041 ccgcatcgcc acgcgcggac tttccttatt gatatgccct tgaaaattca cactttcaaa
     2101 cacaaaccgc ccgttcacat tcagcccccg cgcctgatcc aacacccacc gcatcgcccg
     2161 cgacagcggc acatcatgcc cccgccgctc ctttttccgt tcggcgggga tacgccaaac
     2221 ctccccgtcc aactccgacc actccatcaa cgccgcctcc cgaatccgcg tcatcgtcaa
     2281 cagcagccaa tagatacaaa accgcgttac aggatggata tgcaccctgc ccgcataaac
     2341 cgcaaattca ttgcgctgct ccaaaaaatc aatcaggcgc ggcaattcgg acggactcaa
     2401 cgccgtcatg tttttggttt tcacccgttc aaacacaccc gaaccgattt gcgcgacagg
     2461 gttgatttcc atcattcccg aaccgaccgc aaacgcaaac gcaaacacca tcttcagact
     2521 gtttttcgtt ttgcgcaacg tatcaacgat accgcgcgcc tccatcacac gcaggcagcc
     2581 gaccacgtcc gccgtcctga cttgacggat atcaagattg ccgatagccg gaaaaaccca
     2641 ccgttcaaaa ttccgcataa cctgtccggc atacttttca gaccgcccct tcgaccaacg
     2701 cacaaaccaa tcacgcgcca ccttctcaaa agcaaaatcc gcccgcacct tcttattgac
     2761 gacattttcc ccgtgtgccc gttttcggcg cacctcctcc cgccattccc gcgcctcagc
     2821 cagcgagaaa tcaggatacc gccccagcga aattgtctgt tgccttccgt cctgcataaa
     2881 cgacagcttc caactccgtc cgcccgtcgg gtagacccac aaagccagcc cccccgtccg
     2941 acaacttata cagcttatcg cgcggcttcg catttttaac ctgatttgcc gacagcggcg
     3001 taatgatttt cgccattatg gtaaattccc gtttgtccaa aatttaccat ataaattacc
     3061 ataaaaaaat gcaataagaa gatgccgcaa gattgcaaat gatttgcctt gaagcgttat
     3121 gataggtcaa gccgatgatt tttatatggt aatcaacaaa acttgaacaa aaaagaaccg
     3181 ccctgaatca gggcggttat gttttctggc ggaaacggtg ggattcgaac ccacggagga
     3241 tttgcaccct cagcggattt cgagtccgct gcattcagcc tctctgccac gtttccgata
     3301 atgcagtaaa accaaataaa aataacaata ttcgcggcta ttgcattttt atttggtttt
     3361 acgctcaaaa attggcggaa gcggtgagat tcgaactcac ggagggctat caaccctcga
     3421 cggttttcaa gaccgttgca ttaaaccgct ctgccacgct tccgtcttct tgaagagtag
     3481 gaataataat gaagtttata cattttgcca agcacttttt ttgggaaata cttaatttat
     3541 tgttttcact ttatttgttc cgaacgcata aacacccatt cgctttcatt ggaagccgcg
     3601 tcattgaatg cgtagccttc gtaattaaaa tttttcagca tttcaggatc ggtaatattg
     3661 tgttctgccg catagcgcac catcaatccg cgcgcgcgtt tggcatagaa actgatgatt
     3721 ttatatttac cgtttttttc gtctttaaat atcggggtaa tcagccgcgc ccgaagtttt
     3781 ttcgtgttga cggacttgaa atattcctgt gaggcaaggt tgacaagcgt attgctgcct
     3841 gcttgggcaa gcgtgtcatt taaaaggttg gtaatgatgt cgccccaaaa ctcatacaaa
     3901 ttcttgccgc gcaaattggc aaatgccgtt cccatttcca ggcggtaggg ctgcatcaaa
     3961 tccagtggac gcataagacc gtacaagccg gacagaaggc ggacatggtt ttgcagatat
     4021 tgtatttgat tggtattcaa tgtgtttgca tccatacctt cgtaaacatc gccgttgaac
     4081 ataaagactg cctgtttggc gttttccggc gtaaacggcg tgtgccacgc tgcatttcgt
     4141 tcggcgttta gaagtgcgat tttgtcggaa acgtgcatca actctgcaat ttgttgaggg
     4201 gcaagctccc tcagttggcg catcaggatt tcagattccg ccaaaagatc gggttgggta
     4261 aactcgctga cagggcaagg gtctttttcg ttaaggtttt tcgcagggga aaggacaaaa
     4321 aacataatcc gctcggtata gtgtcaaacg tcggaatttt agatttaaaa gcatcctcag
     4381 gcaagccgat tgattgtgat gtaaataccg ttataataaa tacatcttga tagtattaaa
     4441 aaaagccgtc aggaaaagtc cggacggcat tttttattct ttaccgatat aagcaaacaa
     4501 tggtttccgc agctttgaca caagttgcga catctgcaac caaagcaatc cgcacataac
     4561 cttccccggg attgccctgt tcggtatccc gtgccaaaaa gcgtccgggc aatacttgga
     4621 tagcggcttt ttgccataaa ttgcgtgcga atgccaaatc gtcgccatcg gggactttca
     4681 accagatgta aaacgaggca tccggtaatt taacgtcaaa tacctgttgc aaaatgggaa
     4741 taacacgctc aaatttttcc tgatacaggc ggcggttgtc gataacgtgt tgttcgtcat
     4801 tccaggcggc aatactggca cgctgcacgg gaatgctcat tgcgctgccg tgataggttc
     4861 tgtaaagcag aaagttctta agcagttccg catcaccggc gacaaaaccg gaacgcaggc
     4921 ccggaacatt ggaccgcttg gacaagctgg taaacataag cagtttttgc ctgcttcgcc
     4981 ccgactgtgc ggcggcctgc aggcagccca aaggtttgtt gccgtcaaaa tagatttcgg
     5041 aatagcattc atccgaggca ataatgaaac catatttatc ctgtaaatca aaaacttctc
     5101 tccatccgtc caaatccggc acgctgccgc tggggttgtt gggcgagcag acgaacacca
     5161 gtttggtgcg ttcccaaacc tcttcggaaa tactgcgcca atcggggttg aaagacggcg
     5221 cggggcaatt ggcaaaatgg atttcaccgc cgcccaaaag tgtcgcacct tcataaatct
     5281 gataaaaggg attcgggctg acgattacgg gttttaggcc gtctgaaaca gggttcaaca
     5341 cggtttgaac aaaagaaaac aacgcctccc tactgcccaa aaccggcaga atttcattat
     5401 ccggattgac cgtcaagccg tcataacggc gttttaacca gttcgcacac gcctgacgca
     5461 gttcaggcag acccgcagtc agcggatatt tttccaactc gcgcaatgag gcggtcagcg
     5521 catccgtaat gactttcggt gtcgggtgtt tcggttcgcc gatatgcagt gggacggctt
     5581 ccatgccttc aggcgcggaa atgccctgca tcgcctcgcg cagtcgggca aagggatatg
     5641 gtttgagctg cttgagtaag gtattcatga ttttctccgg ttatcggtat atcggaagat
     5701 atgccgcctg aaccgtcctt cagacggcat tctatatctt tagtgagaac ccgtactgcc
     5761 gaagccgccc tcaccccggc tgcttccgac aaattcctcg acacgtttga agcccgcctg
     5821 tacgaccggc acgataacca tttgtgcgat acgctcgaac ggtttgacag taaacggttc
     5881 gctgccccta ttccataacg acaccttcaa ttctccctga taatcggagt cgattaagcc
     5941 gaccaaattg cccaagacaa tgccgtgttt atgccccaag ccggaacggg gcagcaaaac
     6001 ggcggtatat gcgggattcg ccaaataaat tgccaaacct gtcggcacaa gaaacgtttc
     6061 gcccggctgc aaaacgactt cctcatccaa acaggcgcgc aaatccaaac ctgcagaacc
     6121 ctccgtcgca taggcaggga taaaatccgc catcctctcg tccaacactt tcatttctac
     6181 ttcaatattc atcgtcctgc tcctgcatac ggccggtttc aaagattgga gattatacac
     6241 aagccgtgtt tacgctgaaa cacaacaaac tgcttttgtt ttgaaaccat cctcgactgt
     6301 caaaaacatt ctgcctgtgt atgataccgc cccgtctctt catatataaa cattaacatg
     6361 acgcacacag cctctccacg tgatgaattc atacgcggca tcaaagaaag ttcgcccatg
     6421 ctgattgggc ttttgccttg ggcattgata ctcggaatgc agggcgggca aaaaggtatg
     6481 ggccggctgg aaatgctgct gatgacgggg atgaacttcg ccggcggctc cgaatttgcc
     6541 acggtcaacc tgtgggcgga acctctgccg atactgccta tcgccaccat aacctttatg
     6601 attaattcgc ggcatatcct gatggggggc ggcgcttgcc acgcacatga aagaaatacc
     6661 gctgaaaaaa gccgtgcccg cgctgctttt tatgtgtgat gaaagccggg ccatggcatt
     6721 ctccgaaatc caaaaacgga aagcagccgg tttgcctgca ttcaatatgc ctttttatat
     6781 gggcgcatgc tttatcctct acattacctg gatagggttt gccgccctcg gcgcggcagt
     6841 agggcccgtg ttcggtaatg tggccgcttg ggggttcggt atggcgtttc ctgccgtatt
     6901 tttggtttta ctgaggggaa tgtggaaaag tttttccgca tcaagacttt ggtttgtcaa
     6961 cctgtttgtc gcatccgccg tttacctgac cgttgacggc gcgtggtatg ttcccgccgg
     7021 cacgctgtcc ggcctgttgt ccgcctattt ttggaatggg cggaaatgaa agatttattg
     7081 tcgttcccat cattcctgct gttttcctgt atgcttgccg ttacctgccc tacccagtta
     7141 atcggctttt tcgcattgcg taaccgaacc ttaagccgcc gcgcccaaac cgttatggaa
     7201 accgcgccgg gctgcgtcct catttccgtt attgccccct attttgtctc cgacaagccg
     7261 cacgaactta ttgccatcgc gttaaccgcc tttgcggcct gccgtttttc tatgttgttt
     7321 accgtattga taggggtcgg ctcgtcaggc atctcgggat ggttgatggc ataattcatt
     7381 tcaaaatgcc cataaaaaat accgtctgaa aattgatctt cagacggtat tggcaaaaca
     7441 accctatttt ttacgattag agaaagcttc acagccggta tttgcggaca aggtctccga
     7501 ataggtctgg gcagaagtgg cattacggca tttgtatccg tcgcccacgc tgttcatcca
     7561 taccgacaag gtataacccg tccctgcctt cggaacgccg accaacctgt atgcccttgg
     7621 tttttccgta ttggcaaatg ccaccgaaac actatatttt ttggcaattt tcggattcat
     7681 cttatagcct aagacaaatg tttccagttt gctcttgatg atctgattat tgtcctgggg
     7741 atttttcaaa ataaactgtt tgagaacatt gttgataccg accatctccg tataaagctg
     7801 ggactgatag cctttttcaa tataactttg ataagaaggt atggcaatga cgctgataat
     7861 gccgagtatc gcgacaacta tcatcatctc aatcaatgta aacccttttt gttccatttt
     7921 attgctcatc attattgcct acataagatt gaaggacgac cacggtattg gcattcttac
     7981 cccaagcctt ggcagtaacc cgataaacat tttgtccgtt cttcacgcct aaatattcga
     8041 taatatagcg cggcattttg ctgacttttc ccgtaccttt cttatattcc actccctgat
     8101 tgtcaatgca caggccggta gaatttttgc cagactttgc agggcaagaa cgcttcaccg
     8161 cctcaacggt gggcttgcct tgcaccacga tattgccaaa agcctcttca ttaccattat
     8221 catttgtccg cacattcact gcggtacaca gacctttttc acagttttcg ctaaacgtaa
     8281 ccttactgtc cgcagcatat tccaaatcca aaacctgaaa ttcgccctcc cgcaaagccg
     8341 cctcggctaa agacaaagcc aatttcctgt ctgattcgtt ggcactgatc ctctgttcgg
     8401 tattgtagga ctgggcggca gttacaacca aaaaggccac gactatcatc accatcagca
     8461 cgataaacag tgcggacccc ctctgtccgt cagaagtcgg gattcctgtc aaagtgttct
     8521 gtttgcgcat acatttcccc cgcgtattgt cgcattgata cggtaagcat aaataatatt
     8581 atctgaagaa gcggcaatct tggcatcact accgctatcc aataaaacct ccaccccggc
     8641 aggcgtaaca gagctgtcga atttatccgt atatttgaat tgttcctctt tgccggcatc
     8701 ttcatcttca ggacaaccgg aaacatagat ataccgcact ctcatacgtt taatcttttt
     8761 cgcgagcaac tgaggattac cccactcgcc cttttcattc aattggaagc ggaacaaacc
     8821 ttcctcaccg gcaatcttgc cgaccgcata ggcattgacc acatgccttt gacgcgctat
     8881 attgccattt tgctccttat cagaattctg aatcttcaat tctttttttg catcttctaa
     8941 agtagggatt tgcttacccg gtttcgatat tgcggcacag ctgctgacga cggtagtatc
     9001 ggcgcttgca tcaacatcat cgattccgta ttgaaaaatc aatgcgctac taacctggaa
     9061 aaaattctga taattgatat ttggagattc cgttatgggg ataagtttat ttgtactgtt
     9121 tgtactgttc ctttttaagg aaaaaagagc gtttttttgc gccacattga aaacaacatc
     9181 atttccgata tgctcagaca tattgaaaca accgaagctc cccgccattc ttgcatcgcg
     9241 gacaatcaat gttgccgcat tccgcaaatc ctgttgctcg gcaagacgct cgtttgccgc
     9301 atcatttaat ttccgggatg taaagtaact cgatacgacc gctatcagga caatcacact
     9361 gagcaggccc gcaaccagaa attcaacaat ggtaaaaccc ttcataccat cataaccgcc
     9421 ttttggtacg tttagcattt tacgtttcat tcacgacctc cgacccttgc ctgataggta
     9481 tatacgatat tgccgccgct caccccaaga ttcgtacgga aaatatccga atcccctgcc
     9541 gaatcattta cccacaatac tttaatcaaa gtatccccgt ttgccttctt gtcgcaattt
     9601 gaagaaaaag tattgccgga caatgtcggc gcgtcacccg acgaatcctt gcagacggcg
     9661 taacgaatag ctaccgcatc cggcaaggct tgtttcaatt cataaccgaa cctgtccaac
     9721 tgggcttttg ccaaatccgt cttacttatc aaattattaa gcttgaaatc gccgtcagag
     9781 gaagtggggg ggtacgaccc cgtgtaaaga ttatagtttt tcttgttgct gtccgaatca
     9841 atggtcggat tcatcaacat tccttccatc aggttttgcg tgatttggct gacgatggtt
     9901 tgtgtctccg cctccctgac ggaagcgact gtccgcaact gtacggacag caatgccaaa
     9961 ataccgatgg tcagaacgag catagcgacc aagacttcta tcaacgccat accggactgg
    10021 ggatttttca ggcgcaagca atcattattc ttcatttttt atttcccaaa ccgactaaaa
    10081 cagtcattaa tgtttgcata cggcgcgcgt atcgttttta cgacaaactt cgaccctgcc
    10141 gctgctgtca atcaaaacca ccgccgaacg gaatttctta tcggcatctg aaactgctct
    10201 cgcatctgtc agcacgattt ggatataacc gtcggaataa acaaatttgg aattatcctt
    10261 gagattctga tcgggcaaat agccgaatgt cccgttttgg ttgaacgtcc aaactacacg
    10321 gtcggctttc ggctgagacg aaccgaaagc gatatggttg aaggcataat taatccggct
    10381 gtcgtcggta tcattcaata ccacgttgcg gagaaaaaca tccttagtat cgccgtcata
    10441 tgccttattg ccgtttttgt cgccgaaagc caacattccc cgccccttct tgctgaaatc
    10501 acatccattg ttgggcgcac cgtctttttt aacttgaaca ggacagatat agacagggag
    10561 attgagccga acggcttcgc ccctggaaaa acgcaaaagg ttggcaacct gctccgcgtg
    10621 actggcaatg cggcgtgatg caatccaccc gcccatattg gggagggcta tcgtcgccat
    10681 aatggctgca atggccatca cgatgagcag ctctattagc gtgaaacctt gttgttttcg
    10741 tgtacacata agcaatagga cgttaattgg taatgtatag tggattaaat tcaaaccggt
    10801 acggc
//
LOCUS       DAWTCI010000070        10665 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWTCI010000070 DAWTCI010000000
VERSION     DAWTCI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.070"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(140..1975)
                     /gene="edd"
                     /locus_tag="WM481_001709"
     CDS             complement(140..1975)
                     /gene="edd"
                     /locus_tag="WM481_001709"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFA9080976.1"
                     /translation="MNHTPIHPKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRDELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVVKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFICVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNVREVERIDLGANQQGCGRELFAGFRSMTSSAETGAMSFGGEFA"
     gene            complement(2059..2177)
                     /locus_tag="WM481_001710"
                     /pseudo
     CDS             complement(2059..2177)
                     /locus_tag="WM481_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2495..3940
                     /gene="zwf"
                     /locus_tag="WM481_001711"
     CDS             2495..3940
                     /gene="zwf"
                     /locus_tag="WM481_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFA9080977.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSGEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTIE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFRPLQNHIFDNSQTAPNRLVIELQPNESVRLYTQVKTPGAGNKVEV
                     TPLGVDLGKAVEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGNKWHEEQ"
     gene            4092..4787
                     /gene="pgl"
                     /locus_tag="WM481_001712"
     CDS             4092..4787
                     /gene="pgl"
                     /locus_tag="WM481_001712"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFA9080978.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTNHADSNTGLVREYLLKNKAAAAVWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLILGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERISMTLDAIAHTGHVFLAIQGEEKKAVFDQAAQGENREYPISLVL
                     NHQGVNCHVFYAE"
     gene            4768..5754
                     /locus_tag="WM481_001713"
     CDS             4768..5754
                     /locus_tag="WM481_001713"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFA9080979.1"
                     /translation="MSSTPNKHADYPRLVADIGGTNARFALETAPCVIEKVAVLPCKE
                     YDTVTDAVRAYLNQSGATGVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQALGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSPAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYRHVSAERFLSGAGLSLIYETLAAKQKAEP
                     AKLMPSEITEKALNCESPLCRQALDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRM
                     LDYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGIAGAAAALGNHLKNV"
     gene            5803..6651
                     /gene="hexR"
                     /locus_tag="WM481_001714"
     CDS             5803..6651
                     /gene="hexR"
                     /locus_tag="WM481_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFA9080980.1"
                     /translation="MLSKISESLADLSGAERKVAECALAEPKWFVHAAVAEIADRASV
                     SQPTVIRFCRSLGYKGLPEFKLSLSASIGHEGMPYVHEELNADDDMANVVEKVLGNAA
                     AALLGERRFLKESELENGIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            6877..8523
                     /gene="pgi"
                     /locus_tag="WM481_001715"
     CDS             6877..8523
                     /gene="pgi"
                     /locus_tag="WM481_001715"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFA9080981.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIMVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     TMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNRKAVAEFGIDIANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDRHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMCGKTPDEVRAELKAQGMDEA
                     RIEELVPHKIFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETEPQKHDSSTERLINLYLQTNRK"
     gene            9301..9753
                     /locus_tag="WM481_001716"
     CDS             9301..9753
                     /locus_tag="WM481_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HFA9080982.1"
                     /translation="MALIKEPLDKAKQRNEKLEAAEATAQEAREAEEAAAQEALGREQ
                     EAARVSEWEERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGE
                     TPNGIKVSELACKTAETEARLEELHNRKKALIDEMVREEDKKELPKRL"
     gene            9798..9938
                     /locus_tag="WM481_001717"
     CDS             9798..9938
                     /locus_tag="WM481_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080983.1"
                     /translation="MPQYRNWQSGLDAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            10043..10426
                     /locus_tag="WM481_001718"
     CDS             10043..10426
                     /locus_tag="WM481_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFA9080984.1"
                     /translation="MIYYPAVLFQDPDQSNWGVIIPDLPGCYPVGDTVADALADAKAA
                     AMFHLEGLLQENLPIPEAQSIEVNRDNPDYADAVLWTMVEADDAALTGQVRFNVSWPQ
                     HILNRVDACTAARHETRAVFWRKPP"
     gene            10480..>10665
                     /locus_tag="WM481_001719"
     CDS             10480..>10665
                     /locus_tag="WM481_001719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080985.1"
                     /translation="MPSECGAAAILPRIPFIGGAALVEPRVVLPAFQLADIRLPAVVA
                     VEKYAVDQGIYSGLTKTG"
BASE COUNT         2561 a         2830 c         2948 g         2326 t
ORIGIN      
        1 caacctttgg ggtaaaacat tctgatgttg cttgggtttt gttcgcgggc aaagctcacg
       61 ctacgtctgc atctatttta aacggcttca ataatttttc agacggcctt ctgttttaaa
      121 aggccgtctg aaacgcgcat caggcaaatt ctccgccgaa gctcatcgca ccggtttccg
      181 cactgctggt catactgcgg aagccggcga agagttcgcg gccgcagcct tgttggttcg
      241 cgcccaaatc gatgcgctcg acttctcgga cgttccattc ggcctcgtta atcaggacgt
      301 tgagttcgcc ggtaacggag tcgaagcgaa tcaggtcgcc ggtacggatt ttggcgatgt
      361 tgccgcccat cagggcttcg ggcgtcatgt ggatggacgc gggcactttg cctgacgcgc
      421 cggacatacg gccgtcggtc agcagcgcca ctttgaagcc gcggtcttgc aggatgccca
      481 aaggcggggt cagtttgtgc aattcgggca taccgttggc acgcgggcct tggtagcgga
      541 cgacgcagat aaaatcgcgt tccaactcgc cgcgttcaaa tgccgccaac acttcgcgct
      601 ggtcgttgaa cacgatggcg ggtgcttcga tgatgcggca gccttcgcgt acggctgaca
      661 ctttaaccac gccgcgtccg atgttgcctt tcatcaggcg cagaccgccg tccggggaga
      721 acggattgtc ggctttacgc aggatgtcgt cgttgccgct ggtctcgggg gcttcgcgcc
      781 attcgagttt gccgtcgatg aggaaaggct ctttagtgta gtggcgcata ccgtgtccga
      841 cgacggtatc gacatcgtcg tgcaataagc ctgcgtccag caattcgcgg ataacgaaag
      901 gcaggccgcc tgctgcggta aagtggttca cgtcggcttt gccgttggga tacacgcgga
      961 tgagcagcgg gatgatggaa gaaatttcgt cgaagtcgtc ccagttcaaa atcacgcctg
     1021 cggcgcgcgc catagcgacg aggtgcatgg tgtggttggt cgagccgccg gtcgccatca
     1081 aaccgatcag ggcattgatg aaggattttt cggtcaacat ttcgcccaaa ggtttaatcg
     1141 tgccgttttt gatgccgcgc gcgaggtgtc cggcggcgta gcgggtcagg gcttcgcgca
     1201 ggtcggtata ggggtggaca aaggcggcgg caggcaggtg tacgcccatc atttccatca
     1261 tcatttggtt ggagtttgcc gtgccgtaga aagtgcaggt gcccgggctg tggtaagaac
     1321 ccatttcgct tttcaaaagt tcgtcgcgtc cgactttgcc ttcggcgaaa agttggcggg
     1381 tacgggcttt ttctttattg ccgatgccgc tggacatcgg gcctgccgga acgaagatac
     1441 cgggaatatg accgaacgaa agcgcaccta tcatcagacc cggaacgatt ttgtcgcaca
     1501 cgcccataaa caggctgccg tcaaacattt ggtgcgacag cccgacggcg gtactcatcg
     1561 caatcacgtc gcgggaaaac agcgacaatt ccatgccggc gtagccttgc gtgatgccgt
     1621 cgcacatggc aggcgtgccg ccggcgactt gtgcggtcgc gccgtttttt tgtgcttcgt
     1681 ctttgatttg gtcagggaag tctttaaacg gctggtgtgc ggaaaccatg tcgttgtagg
     1741 cagtgatgat gcctaagttg gggacggttt cctgaagcat ttcgattttg atgcttttgg
     1801 gcatggcggc ataaccgtgc gccaaattgc tgcagccgag ctggttgcgc tctaagcgtc
     1861 ccatttgttt ggcactgcgg attttcgcca gatatttttc acgcgtcgga cggctgcgtt
     1921 cgataatgcg cccggtgatt tcggcgagtt tggggtggat aggagtgtgg ttcatgttcg
     1981 gtctcctggc ggaacggggt ggtattgggg atttgtttgt gcgtttctga tttttgtatc
     2041 gggtatgtta gacggtgagt atagtggatt aaatttaaac cggtccggcg ttgcctcgcc
     2101 ttgccgtact atttgtgctg tctgcggctc gccgccttgt cctgatttaa atttaattca
     2161 ctatatttta aaaggtacgc cgtctgaaag tgggaagcgg gtgctattat agtacaaaat
     2221 ttgtaatttt gttacaaaaa aatcagacat tgaaaagaaa tttgatttaa atcgcagaat
     2281 cttctgattg ttgtaacgga ataggggaaa cctgcctgat ttttggacta ggtcaaataa
     2341 attgtaaaaa tgggaataga caaaaagtgt agcggtgtgt agtattacta ctcattatgc
     2401 cgcctgcctt tgtttccgat attgcgatgc tgcgtgatgg aaaaaacagg tttcagacgg
     2461 catcgaaaaa atcctttcct gacgagataa tgcgatgagt acacagacaa attttgattt
     2521 ggtgttgttc ggtgcgaccg gcgatttggc aatgcgcaaa cttttgccct gcctgtatca
     2581 ggcgcatgtt gccggtctgc ttcatcccga agggcgtatt ttgggggtca gccgcagcga
     2641 gttggatacc gaaggttttc tggcgaaggt ggaaaccagt tccaaaatcc atgtgaaaga
     2701 aaatttttcc ggtgaggcgt gggcttcgtt tgttgaacgt ctcgcatatc tcaaagtcga
     2761 tgttacgcag ccggacgatt ttgccgcttt gggcgatttg gtcaaagcac gcaaagaaac
     2821 cgacaacgtc gtcatctatc tgtccaccgc gccgaaattc ttcgcgcaag cctgcgaaaa
     2881 ccttgctgcg atcggtttga atgccgacaa tgtgcgcgtg gttttggaaa aaccgctggg
     2941 tacggatttg gcttcttccc aacaaatcaa taccgatgtg gcgcgttatt tcaaagaagg
     3001 gcaaatttac cgcatcgacc actaccttgg caaagaaagc ctgcaaaacc tgcttgcgct
     3061 gcgttttgcc aatgtaatgt tcgagccgct gtggaacaat aaatacatcg aaagcgtgca
     3121 gctgaccatc gccgaacagc tcggtgtgga agagcgcggc gagttttacg acattaccgg
     3181 cgcgttgcgc gatatggtgc aaaaccattt gatgcagatg ctgtgcatga ctgcgatgga
     3241 agcccccgcc agcttggatg ccgacgcggt gcgcgatgaa aaagtcaaag tcatcaagtc
     3301 attgaagccg ctgaccatcg aatctgtcaa tgaaaatgtc gtgcgcggac aatataccgc
     3361 tgccaaaggc atgaacggct atcttgaaga aatcaacgtt ccgcaagaca gctttaccga
     3421 aacctacgtc gccattaaag ccgaaatcga aaacgaacgc tggaagggcg ttcccttcta
     3481 cctgcgtacc ggcaaacgca tggcgggcaa agtggcggaa atcgttttga acttccgccc
     3541 gctgcaaaac catattttcg acaacagcca aaccgcgccg aaccgtttgg ttatcgagct
     3601 tcaaccgaat gaatccgtcc gcctttacac gcaggtgaaa accccgggtg cgggcaacaa
     3661 agtggaagta acgcctttgg gcgtggattt gggcaaggcc gttgaaggcc gccgcgccga
     3721 ggcttacgaa cgcctgctgt tggatgtgat taacggcaaa ctcgctttgt tcaaccgccg
     3781 cgacgaactc gaagccgcgt gggaatatgt gatgccgatt ttggaaaact gggcaaataa
     3841 caccacgccg ccgcacggtt acggcgcaca ctcttggggt cctgaagccg cccgcgaact
     3901 gttggcgcgc gacggcaaca agtggcacga agaacagtaa tgcgcttcag acggcatcgg
     3961 gttcgcaatg ccgtctgaag gagggcgggg cagaaaaagc agtcccgctt cgaggcggaa
     4021 gcggctaagc cattgcccgg cagccgccct gtaacgggat atgccgtctg aaatcacaaa
     4081 aggacacaaa tatgtttgtt tggcacgaat acgaaaatgc ggcagaagcg gcgcagtctt
     4141 tggctgacgc agtggcggat gctttgcagg gcgcactgga cgagaagggc ggtgcggtgt
     4201 tggcagtttc cggcggacgt tcgccgattg catttttcaa cgccctgtcg caaaaagatt
     4261 tggattggaa aaacgtcgga attaccttgg cagatgaacg catcgtgccg accaaccacg
     4321 ccgacagcaa taccggtctg gtgcgcgaat acctgttaaa gaacaaagcc gctgcggcag
     4381 tgtggattcc tatggtggaa gacggaaaaa ctgaaaccga attacatccc gatgctgttg
     4441 tcgattatgc gctgaaacat tacaagcagc ccgatgtttt gattttgggt atgggaaacg
     4501 acgggcatac ggcttcgatt ttcccaaaag ctccgcagtt tcagacggca atcgacggtt
     4561 cggcaggtgt cgcgctggtg cataccacgc ccgttaccgc gccgcacgag cgcatcagca
     4621 tgactttgga tgcgattgcc catactgggc atgtattttt ggcgatacag ggcgaagaga
     4681 aaaaagccgt gttcgaccaa gccgcacaag gcgaaaaccg cgaatatccg atcagcctcg
     4741 ttttgaacca tcaaggagtg aactgccatg tcttctacgc cgaataaaca cgccgattac
     4801 ccgaggctgg ttgccgacat cggcggcaca aacgcccgtt tcgcattgga aaccgcgccg
     4861 tgcgtgattg agaaagtcgc cgtcctgccg tgtaaggaat acgacacggt taccgacgcg
     4921 gtgcgtgcct atctgaacca aagtggtgca acgggcgtac ggcacgcggc atttgccatc
     4981 gccaacccga ttttgggcga ctgggtgcag atgaccaacc accattgggc gttttccatc
     5041 gaaaccaccc gtcaggcttt ggggctggac accctcatcc ttttgaacga ctttaccgcg
     5101 caggcattgg cggtaacgca gacttcaagc aaagacctga tgcaggtagg cgggcaaaag
     5161 cctgtcgaat tcgcccccaa ggccgttatc ggccccggta ccggcttggg cgtgagcgga
     5221 ttggtgcaca gccccgcagg ctgggtggcg ttggcgggcg agggcgggca taccagtttc
     5281 ccgccgtttg acgatatgga agtgttgatt tggcagtacg ccaaaaacaa ataccgccac
     5341 gtttccgccg aacgtttcct cagcggcgca ggtttgagcc tgatttatga aacgctggcg
     5401 gccaagcaga aagccgaacc ggcaaaactg atgccgtctg aaatcaccga aaaggcatta
     5461 aattgcgaat cgcccctgtg ccggcaggca ttggacattt tctgcgcgat gctgggcacg
     5521 gtcgcttcca acctcgccct gactttgggc gcgcgcgggg gcgtgtattt gtgcggcggc
     5581 attatcccgc gaatgttgga ttacttcaaa acctccccct tccgcagccg ctttgaaaac
     5641 aaagggcgtt ttgaagccta ccttgccgcg attcccgttt atgtcgtctt gagcgaattt
     5701 ccgggtattg ccggcgcggc ggcggcgctc ggcaaccacc tgaaaaacgt ctaaccacgg
     5761 cagcccttgc agcagggccg cattatcgaa gggcatatca ttatgttaag caaaatcagc
     5821 gaatcactgg ccgacctgtc cggtgcggaa cgcaaagttg ccgaatgcgc cttggcagaa
     5881 cccaagtggt ttgtccacgc cgccgtcgcc gaaatcgccg accgcgcctc ggtcagccag
     5941 ccgaccgtca tccggttctg ccgcagcctc ggttataagg ggctgccgga attcaagctc
     6001 tccctgtccg ccagcatcgg gcacgaaggt atgccttatg tccacgaaga actcaatgcc
     6061 gacgacgata tggcgaatgt ggttgaaaaa gtgttgggca acgccgccgc agccttgttg
     6121 ggggagagga ggttcttaaa agaatccgaa ctcgaaaacg gcatcgccac cctgatgcac
     6181 gcccgccgcg tcgaatttta cggcgtgggc aattccggca ttgtggcaca ggacgcgcag
     6241 cacaaatttt tccgtttcgg catgtccacc gtcgcctatg tcgatacgca cacgcaactg
     6301 atggcggcat ccgttttgag cgatcaggat gttttggttg ccatttccaa cacgggttcg
     6361 tctatcgaac ttttggatgc ggtcagcatc gccaaagaaa acggcgcgtc cgtcatcgca
     6421 ctgacccgca acgattcgcc tctggcgcaa cttgccgact gcgtgttgag cgttgccaca
     6481 caggaaaacg ccgaactcta cacgcccatg gtttcccgcc tcttgcagct tgccgtcatc
     6541 gatattctcg ccatcggact tgccctgcgc ttgggcgatg ctgccagcct gcaattgcag
     6601 aaaagcaaaa aaagcataca caacaagcac atcgattacg acaaagactg atgttcagac
     6661 ggcatcccgc caatgccgtc tgaacggccg gacaggctcc ggcaagcctt ttatcagaat
     6721 ttccttaatg gcggaaaccc cgccctttaa ggcggcaggg ttcgggtttg ccgcaacccc
     6781 ttccaaatcg gggcggtacg cagggttgcg ccttatgtgc gcatcgatgt gcggaaacgc
     6841 cccccgtcac ggaaaacctc aatcgggagc atacagatga aacaccttca cgatttaccg
     6901 gcctggtcaa aattgtggaa ccactttgac gacagcaaaa cattgcatat gcgcgaaatg
     6961 ttcgagcaag acccgcagcg ggcggaacgc tactggctgc aagtcggcgg actgacgctg
     7021 gactactcca aaaaccgcat caacgacgaa accatgtccc tactgttcga gttggcgcgt
     7081 gaagcaggcg tgccggagcg gatgcggcag atgttccacg gcgaaaaaat caataccacc
     7141 gaaaaccgcg ccgtcctgca tgtcgccctt cgcaaccgca ccaattcgcc gattatggtt
     7201 gacggtgaag atgtgatgcc caaagtcaac cgcgttttgc aacgtatggg cgaatttgca
     7261 cacgaagtcc gcagcggaag ctggctgggc tataccaacc aagtcattac cgacgttgtc
     7321 aacatcggca tcggcggatc ggatttgggc ccgctgacga tgtgtaccgc gctcaaacct
     7381 ttcggtcatc cgcgcctcaa tatgcacttc gtctccaacg tggacggctc gcaactgcgc
     7441 gacgtattgt ccaaagtcca cccggaaacc acgctgttca tcatcgcctc caaaacattt
     7501 accacgcagg aaacgctgac caacgcgcta accgcgcgcg aatggttttt gaatcatgcg
     7561 ggtgacgaag aagccgttgc caaacacttc gccgccgttt ccaccaatcg aaaagccgtc
     7621 gccgaattcg gcatcgacat cgccaatatg tttgaatttt gggattgggt cggcggtcgg
     7681 tacagcctgt ggtccgccat cggattgccg attatgctgt atctcggcga agaaaacttc
     7741 atcgaaatgc tcaacggcgc gcacctgatg gaccgacact tcatcaacac cccgctcgag
     7801 cgcaacctgc ccgtcatcct cgccctcatc ggcatctggt atatcaacta ctacggtggc
     7861 ggcagccacg tcatcgcgcc ttacgaccaa catttgcacc gcctgcccaa attcatccag
     7921 cagctcgata tggaaagcaa cggcaaacag gttacgttgg acggcaaagc agtcggacac
     7981 gaaacctcgc cgattatctg gggagaaacg ggcattaacg gtcagcacgc cttcttccaa
     8041 ctgctgcatc aaggcacgca catcaccccc atcgacctga ttgcctcgct tgaaaaacgc
     8101 agcaacctgc ccggacacca cgaaatcctg cttgccaacg tctttgcaca agcagaagcc
     8161 tttatgtgcg gcaaaacccc cgacgaagtc cgcgccgaac tcaaagcgca gggtatggat
     8221 gaggcgcgca tcgaagagtt ggttccgcac aaaatcttct ccggcaaccg cccgaccaac
     8281 ctcatcctga tggataaggt caacccccgc aatatgggca gcctgattgc catgtacgaa
     8341 cacaaaacct tcgtccaagg catcatttgg ggcatcaaca gcttcgacca gtggggcgtg
     8401 gaactcggca aacaactggc taaaaccatt ttgggcgaac tgacaggcga aaccgaacct
     8461 caaaaacacg acagctcgac cgaacgcctg attaacctct acctgcagac caaccgcaaa
     8521 taaaacctgc ggaaaaatgc cgtctgaacg ccgaccgttt cagacggcat ttttatcgaa
     8581 caggaaaacc gtcggtaaat ttgcagggcg tgtgcaatcg cgatatggcg gtttgcataa
     8641 atttaaacat atatgttccg cagccatgta cttcaagtgc cgtagcgtgt gtggaaatac
     8701 gcaacagaca gtccgtgttt ggacggcaaa ggaaatctaa tgaaaaaaaa tttacccgca
     8761 ctggcattgg caagtatgct gattttgtcg ggctgcgacc gtttgggaat aggcaacccg
     8821 ttttccggaa aggaaatttc ctgcggaagc gaagagacta aagagatttt ggtcaaactg
     8881 gtccgcgaca atgtcgaagg tgaaaccgtc aaaacttttg acgacgacgc attcaaagac
     8941 caagcatttg ccgatatcgg catatcgcat atccgcagaa tggtcgaacg tttgggcata
     9001 accgtcgatg aagtccgaac taccgagaaa accgacacgt ccagcaaact caaatgtgaa
     9061 gccgcgttaa aactggacgt gcccgacgat gttgtcgatt atgccgtcgc cgccaaccaa
     9121 tctataggca acagctataa gaaaacgccc gacttttttg aaccctacta ccgcaaagaa
     9181 ggcgcgtatt atgtcaaaac tatttcttac agcgtccagc cgacagacga caaaagcaaa
     9241 atctttgccg aactcagtca ggcacacgat atcatccatc cgctcagcga gctggtgtct
     9301 atggcactga ttaaagagcc gttggacaaa gcgaaacaaa ggaacgaaaa acttgaagcg
     9361 gcagaagcca ccgcgcagga agcgagggag gcagaagaag cggcggcgca ggaggcattg
     9421 ggtcgggagc aggaagccgc ccgcgtatcc gaatgggaag aacgctacaa gctgtcgcgc
     9481 agcgagttcg agcagttttg gaaaggattg cctcaaactg tacagaataa gctgcaagcc
     9541 tcgcagaaaa catggaaaag cggtatggac aagatctgtg ccaacaatgc gaaagccgaa
     9601 ggtgaaacgc caaacggcat aaaagtcagt gagttggcgt gtaaaacggc agaaaccgaa
     9661 gcacgcttgg aagagctgca caaccgtaaa aaagccctta tcgacgaaat ggtcagggaa
     9721 gaggacaaga aagaactgcc aaagcggctc tgaacagcgc ggttcaggca ctgcccgccg
     9781 atattgccga aaccgttatg ccccagtacc gcaactggca aagcggtttg gatgccaaat
     9841 gcgccgacgg cgacgaatac ggggtggcac aatctgactg ccgtaccaga gaaatcaatg
     9901 cgaaaaccaa agaaatccaa ggttatctga ttgactgaaa cttggatgcg ggaatgtcgg
     9961 cggcttttgc gtttttgtcg tttttaaatc cccttcgttg cgtttggact ttgccgatac
    10021 cgcatatccg agaaaggaag taatgattta ctatcccgcc gtgctgttcc aagaccccga
    10081 tcaaagcaac tggggcgtga ttattcccga tctgccgggc tgctatcctg tcggcgatac
    10141 ggttgccgac gcgcttgccg atgccaaagc cgccgcaatg ttccatctcg aagggctttt
    10201 gcaggaaaac ctgccgattc ccgaggcgca aagcatagag gttaatcggg acaatcccga
    10261 ttatgccgat gcggtgttgt ggacgatggt tgaagcggac gatgccgcat taaccggaca
    10321 ggtgcggttt aacgtcagtt ggccgcaaca tatcctcaac cgtgtcgatg cctgcactgc
    10381 cgcccgtcat gaaacccgag cggttttttg gcgaaagccg ccttaaaggc gatgcagcct
    10441 taactttcag cccgattttt tcgggctttt ttattgccga tgccgtctga atgcggcgcg
    10501 gcggcaattt taccgcgtat ccctttcatt ggcggcgcgg ctttggttga accgcgcgtc
    10561 gtgctgcctg cctttcagtt agctgatata cgcctgccgg cagtggtggc ggtcgaaaaa
    10621 tacgccgttg atcagggtat atatagtgga ttaacaaaaa ccggt
//
LOCUS       DAWTCI010000071        10552 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWTCI010000071 DAWTCI010000000
VERSION     DAWTCI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.071"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(67..1153)
                     /locus_tag="WM481_001720"
                     /pseudo
     CDS             complement(67..1153)
                     /locus_tag="WM481_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
     gene            complement(1090..1305)
                     /locus_tag="WM481_001721"
     CDS             complement(1090..1305)
                     /locus_tag="WM481_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HFA9080986.1"
                     /translation="MSTQFDVAVIGAGPAGSAASALLRKKGYQVCVLENSTFRASSSA
                     KACCRTVWKCWKKPVLPMPFAPGPAFS"
     gene            complement(1485..2858)
                     /locus_tag="WM481_001722"
     CDS             complement(1485..2858)
                     /locus_tag="WM481_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFA9080987.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGVNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKAEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTFQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYI
                     HRIGRTGRAGADGVAISLMDESEQKMFEAIKELTGNKLLIERIEGFEPRWWEQEGSKP
                     EKTETSEPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRRGHRTCALL
                     QPRYGVK"
     gene            3072..4076
                     /gene="dusB"
                     /locus_tag="WM481_001723"
     CDS             3072..4076
                     /gene="dusB"
                     /locus_tag="WM481_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFA9080988.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRAFGAGWAVCEMLAS
                     DPTLRNTGKTLHRSDFADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPA
                     KKVCNVQAGSALMQDEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPAIAKIAED
                     CGIAALAVHGRARTQMYKGEARYELIAETKSRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECRAAILNHIRAMHAFYGETVGV
                     RIARKHIGWYIGEMPDGEQARREINRLDNAAAQYDTLAGYLERLAGKTDRWACGYREG
                     "
     gene            4089..4238
                     /locus_tag="WM481_001724"
     CDS             4089..4238
                     /locus_tag="WM481_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9080989.1"
                     /translation="MPPFGFGRHLSAWFGGRAESRPANVENNKCRLKTSGTGFAKRLS
                     DGIPS"
     gene            4265..4504
                     /locus_tag="WM481_001725"
     CDS             4265..4504
                     /locus_tag="WM481_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFA9080990.1"
                     /translation="MNPATADIAQCIEQNLNQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            4507..5043
                     /gene="ruvC"
                     /locus_tag="WM481_001726"
     CDS             4507..5043
                     /gene="ruvC"
                     /locus_tag="WM481_001726"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFA9080991.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADEP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            5094..5963
                     /locus_tag="WM481_001727"
     CDS             5094..5963
                     /locus_tag="WM481_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HFA9080992.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIN
                     LAKCFPEWDEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAKCLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNNSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLQFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            6867..7661
                     /locus_tag="WM481_001728"
     CDS             6867..7661
                     /locus_tag="WM481_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFA9080993.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAVSCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELVEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDTGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(7710..7814)
                     /locus_tag="WM481_001729"
                     /pseudo
     CDS             complement(7710..7814)
                     /locus_tag="WM481_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7886..10489
                     /gene="pepN"
                     /locus_tag="WM481_001730"
     CDS             7886..10489
                     /gene="pepN"
                     /locus_tag="WM481_001730"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFA9080994.1"
                     /translation="MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQR
                     AGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEFSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRA
                     VRRIENIRLLRQNQFPEDAGPTAHPVRPVSYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAKQENQSYEYSPETADWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNCLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAQD
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQELQCIRAQEGLSK
                     DVGEIVGKILG"
BASE COUNT         2422 a         2753 c         2998 g         2379 t
ORIGIN      
        1 tgaatgatga aataaaaatg ccgtctgaac ccgattggat tcagacggca ttttgtatgg
       61 caggatttat tcgctttcca actgaccgac ccattctgac aaggcagtca ggcgttgttc
      121 ggatttcgcc acgaacgggt tttcggtatc ccacgcgtag cctgccaaaa tcgaagaaag
      181 catacggctg atttccgggc tgcggtcggg cgcgtacacg acgttttgga agcggaaatc
      241 ataccagccg tccacatagg tgcggaacgc gtctacgccg atcatcaggg gttcggcaaa
      301 ttcggtttgc caatcggcgg cttcgccttt gagttgtttt gtcagcagat cggcagcaag
      361 ttcggcggag tgcagcgcga tggttacgcc cgacgagaac acggggtcga ggaactcggc
      421 ggcattgccc aacagcgcga aatgcctgcc gtgcagtgat ttgacgttgg cggaatagcc
      481 ttggatggag cggaacggaa aatcgttttc ccaaacggct ttgtccaaaa tttcgctcaa
      541 catcgggcat tcgtaaacaa attttttcaa caccgtttcc gattcgccgg caagtttgtc
      601 gggtgtgccg accacgccga cggaacagcg gttgtcgccg aagggaatca gccaaatcca
      661 tacgtcgcgg ttgttgcgga tgggtggtaa tcaggatttt gttgcggtcg aatttcgggt
      721 gggtaatgtt gtcgtcgatg tgcgtgaaat gcgtttggcg cggcggcagg tgcgagggcg
      781 tttccaagtt tagcaggcgc ggcagcacgc gtccgtagcc gcttgcgtcc aagacgaatt
      841 tcgcggtcag ttcatagctc tcgccggtgt cggtttcgat gttcaagcgg gcaaaatcgc
      901 cgctgttgtc gaacgcggtt acgccgtgcc cgaaacgtac ttcaacgcct tgtttggcgg
      961 cttcttcaat caggattttg tcgaacacgg cgcggcggac ttggtaaacc gtgccggggc
     1021 cgtctgaaaa tttatcggtg aaatcaaact cggtatagcg gctgccccac gaaaacgccg
     1081 caccgttttt caactgaaag ccgggcccgg tgcgaacggc atcggcaaaa ccggcttctt
     1141 ccagcatttc catacagtgc ggcagcaggc tttcgccgat gacgaagcgc ggaaagtgct
     1201 gttttccaac acgcagactt gataaccttt tttgcggagc agggcggatg cggccgaacc
     1261 tgccggacct gcgccgatta cggcgacatc gaattgggtg gacattggga aatcctgatg
     1321 gtttgagtgg aaaaggaaat gatattgtac aggcaggaaa tgccgtctga aactgtttgc
     1381 ggaagaaatt tatccggtgc gggcggttgc tcccaatgcg tcagtccggt ttgaaaaatg
     1441 ccgtctgaaa cgggaaatgt tcagacggca tttgattttc agggttattt tacgccgtaa
     1501 cgcggttgga gcagggcgca cgtccggtgt ccccggcggc ttcggcggct gcgcccggca
     1561 atttttccgc aggccgcgcc cgcatcgttt gccgtggttt ccggccgggt atttttttcg
     1621 cgctgagccc tggcggattc gtagcggttg cgttgtctcg gttcgcttgt ttcggttttt
     1681 tccggttttg agccttcctg ttcccaccat cggggctcga agccttcgat gcgttcgatg
     1741 agcagcttgt tgccggtcag ttctttgatg gcctcaaaca ttttctgttc ggattcgtcc
     1801 atcagggaaa tcgccacgcc gtccgcaccg gcgcgccccg tgcgtccgat gcggtggatg
     1861 tagtcttcgg gctgggcggg catttcgtaa ttgatgacga agggcagttc ggcgatgtcc
     1921 agcccacgcg cggcgatatc ggtggcgacg aggacgcgca ggctgccgtc tttgaaggcg
     1981 ttgagtgttt cgagcctgct ttgttgggaa cggtcgccgt gtatcgcctg tgcggacagg
     2041 ttgcggcgca ccagttcgcg cgttacgcgg tcgacgcttt gtttggtttt gcaaaagacg
     2101 ataacttggt tcatatgcag atcgacaatc agccgttcga gcaggttgcg cttttggaag
     2161 gtatcgacgg cgatgatgtg ttgttcgacg ttggcgttgg tggtgttttg ggcggcaacc
     2221 tcgacggttt cgggcgcgtt catgaagtct tgcgccagtt tgcgtatcgg ggcggagaag
     2281 gtggcggaaa agagcagggt ttggcgttgg cggggcagca tctgcatgat tttgcggatg
     2341 tcgtcgataa accccatatc caacatgcgg tctgcttcgt ccagaacgac gatttcggct
     2401 ttgtttaaac tgatgttttt ctgtttcaca tggtcgagca gccgtccgac ggtggcgacg
     2461 actatttcgc agccggcacg caggtcggcg gtttgtttgt ccatgttgac accgccgaag
     2521 aggacggtat gccgcagcgg caggttttta atgtagccct gcacgttttg gtcgatttgg
     2581 tcggcaagtt cgcgcgtcgg ggtcaacacg agcatacgca cggggtgcat tgcaggcgag
     2641 gtgctggcgg tggcgtaacg tttgaggcgt tccagactgg gcagcataaa ggcagcggtt
     2701 ttgcctgtgc cggtttgcgc ggcggcaagc aggtcgtgtc cggccagtgc tttgggaatg
     2761 gccgcggctt ggatgggcgt cgggttttcg taaccttgcg cggtcagtgc ggaaaccagt
     2821 tccgtaccca aacctaaaga ggaaaatgga ttactcatga ttgtagtctt tctttcagac
     2881 cttatgccgt ctgaagcggg aaaccgatag gacggggaaa taaactgccc gcctgtgaac
     2941 ggcggggacg ggaatttgtg tgcggattat gccataaaac ggtgcccgcc ccaatcgcgg
     3001 gcgcgtccgc agattggaaa tcctgcttaa aaaatgtaca atggcgcact tttttgaaac
     3061 gcgggcccat tatgcacatc ggcggttatt ttatcgacaa ccccatcgca cttgcgccga
     3121 tggcgggcat taccgacaaa cccttccgcc gcctctgtcg ggcgtttggc gcaggttggg
     3181 cggtgtgcga aatgctggcc agcgatccga cgctcaggaa taccggaaaa accctgcacc
     3241 gcagtgattt tgccgatgaa ggcggcatcg ttgccgtgca gattgccggc agcgaccccg
     3301 aacagatggc ggatgcggcg cgttacaacg tcggactcgg ggcgcaggtc atcgacatca
     3361 atatgggctg ccccgccaag aaagtgtgca acgtccaagc cggtagcgcg ctgatgcagg
     3421 acgagccgct ggttgccgcc attttggagg cggtggtcaa ggcggcgggc gtacccgtta
     3481 ccctcaaaac ccgtttgggt tggcacgacg accatcaaaa cctgcccgcc atcgccaaaa
     3541 tcgccgaaga ttgcggcatt gccgcccttg ccgtccacgg gcgcgcgcgc acgcaaatgt
     3601 acaaaggcga ggcgcgttac gaactcatcg ccgagaccaa aagccgtctg aacatcccgg
     3661 tctgggtcaa cggcgacatc acttcgccgc aaaaagccgc cgccgtcctc aaacaaaccg
     3721 ccgccgacgg catcatgata gggcgcggcg cgcaaggcag gccgtggttt ttccgcgatt
     3781 tgaagcatta tgccgaacac ggcgttttac cgcctgcctt gagtttggca gaatgcagag
     3841 ccgccatttt gaaccacatc cgcgccatgc acgcgtttta tggtgagacc gtcggtgtgc
     3901 gcatcgcacg caaacacata ggctggtaca tcggcgaaat gcccgacggc gaacaggcgc
     3961 ggcgtgaaat caaccgcttg gacaatgcgg cggcgcaata cgacacactt gccggttatc
     4021 ttgaaaggct tgccggaaaa accgaccgtt gggcgtgcgg ctatcgggaa gggtagggca
     4081 gtattgccat gccgccgttc gggttcggac ggcatctgtc tgcatggttc ggaggtcggg
     4141 cggaatccag gccggcgaat gtcgaaaaca ataaatgccg tctgaaaacg agtggaacgg
     4201 gtttcgccaa aaggctttca gacggcattc cgtcttaaaa acatttcaat caaaaggata
     4261 tgcgatgaac cctgcgactg cggacattgc gcaatgtatc gagcagaact tgaaccaata
     4321 tttcaaagac ctgaacggca ccgaaccttg cggcgtgtac gatatggtac tgcatcaggt
     4381 ggaaaagccg ctgctggtgt gcgtgatgga gcaatgcggc ggcaaccagt ccaaagcctc
     4441 cgtgatgctg ggactgaacc gcaatacctt gcgtaagaaa ctgattcaac acggtttgct
     4501 gtgaatatgt cggcaaccgt ccgtatcttg ggcatcgacc cgggcagccg cgtaacgggt
     4561 ttcggcatca tcgatgtcag ggggcgcgat catttttacg tcgcctccgg ctgcatcaaa
     4621 acgcctgccg atgagcctct ggcagacagg attgccgtga ttgtgcggca tatcggcgaa
     4681 gtcgttgccg tttacaagcc gcaacaggcg gcggtggaac aggtgttcgt caacgtcaat
     4741 ccggcatcga cgctgatgct cggtcaggcg cgcggagcgg cattggcggc tttggtcagc
     4801 cataagctgc ccgtttcgga atacacggcc ttgcaggtca aacaggcggt ggtcggcaaa
     4861 ggcaaggcgg cgaaagaaca ggtgcagcat atggtggtgc aaatgctggg actttcggga
     4921 acgccgcagg cggatgcggc ggacggtctt gccgtcgcgc tgacccacgc cttacgcaac
     4981 cacgggcttg ccgccaaact caatccttcg gggatgcagg tcaagcgcgg aaggtttcaa
     5041 tagtttcaga cggcatttgt attttgccgc ctgaaaagaa aatgtgtacc gagatgaaat
     5101 ttatattttt tgtactgtat gttttgcagt ttctgccgtt tgcgctgctg cacaagattg
     5161 ccggcctgat cggttcgctt gcctaccttc tggtcaaacc gcgccgccgt atcggcgaaa
     5221 tcaatttggc aaaatgtttt cccgaatggg acgaagaaaa gcgtaaaacc gtgttgaaac
     5281 agcatttcaa acacatggca aaactgatgc tcgaatacgg cttatattgg tacgcgcctg
     5341 ccaaatgcct gaaatcgctg gtgcgctacc gcaataagca ttatttggac gacgcgctgg
     5401 cggcggggga aaaagtcatc atcctgtacc cgcactttac cgcgttcgag atggcggtgt
     5461 acgcgcttaa tcaggatgtc ccgctgatca gtatgtattc ccaccaaaaa aacaagatat
     5521 tggacgaaca gattttgaaa ggccgcaacc gctatcacaa cgtcttcctt atcgggcgca
     5581 ccgaagggct gcgcgccctc gtcaaacagt tccgcaaaag cagtgcgccg ttcctgtatc
     5641 tgcccgatca ggatttcgga cgcaacaatt cggtttttgt ggattttttc ggcattcaga
     5701 cggcaacgat taccggcttg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg
     5761 ccattcccgt ccgcgaggcg gacaatacgg ttacattgca attctatccc gcttggaaat
     5821 cctttccgag tgaagacgcg caagccgacg cgcaacgtat gaaccgcttt atcgaagaac
     5881 gcgtgcgcga acatccggaa caatatttct ggctgcacaa gcgtttcaaa acccgtccgg
     5941 aaggcagccc cgatttttac tgactacata aaattacaaa acaaatcagg cgtttcagat
     6001 caaaaacccc gattgttttt gggaatttga aacccgggtt gtacaaacag gatttgccgg
     6061 acggttttaa cggttcagtt gtttgtaaaa acaatgcttt tttaaaattg acaaaaaacg
     6121 aaatcggttt taaaggctta ttccgagaac aaaggggagt ggatgccgaa aacccggtta
     6181 atatattata gtggattaac aaaaaccaat acggcgttgc ttcgccttag ctcaaagaga
     6241 acgattccct aaggtgctga agcaccaagc gaatcggttc cgtactattt gtactgtctg
     6301 cggcttcgcc gccttgtcct gatttttgtt aatccactat aaaattaaat ttgtttaaaa
     6361 acataaagtt gtaaacaagt atctcatata agcctttttc attaaacaga tagtcagata
     6421 ttttgtgcta aaaatttata taatatttaa attaatatca agttataaaa aatatatgga
     6481 attttatttt gtttatttat aattttaagc aataatctaa atcaggcatt tttattcccc
     6541 tgttttaaaa aatatttggc aaggtgtgaa aaaaggcgta cattccgcta cacagaatta
     6601 cagatacagc ggagcaatgc cgtctgaaag gattttccgg tcagtcttgc ggttggtcgg
     6661 ggtttcatcg gatacggtga aacgaaagtt tgccggcgca gggttgagct acgcgggtaa
     6721 agccgcaggc gaaagcctgt attgtttgtg aagcgtaaat ctctgatttg aggtattggg
     6781 gcaatcctgt gggggttgcc tcttttttta tccgcctttt aatgacacaa taggcgcaag
     6841 tctgtttttg aatgcaaggt actgccatga atacgatttt ggctttcgat attgaaaccg
     6901 tacccgatgt gcagggtatc cgtacgttgt atgacttgcc gtcttccctg ccggatgacg
     6961 aagtggtgct gttcgcgcag cagaaacgcc gcgcacagac cggcggcgat tttatgcagc
     7021 accacctcca tcaggttgtg gcggtttcct gctgtatgcg ctgggggcag gacaaggttc
     7081 atgtcggcac catcggcgag atggacgatg gcgaggaagt ggttatcgcc aaatttttcg
     7141 agttggtgga aaaacatacg ccgcaactgg tcagttggaa cgggggcggg ttcgatttgc
     7201 ccgtactgca ttaccgctcc ctgatatacg gcatcaacgc cgcgcgctat tgggatacgg
     7261 gcgacggcga tttcggcgac agccgcgatt tcaagtggaa caactacatc agccgttatc
     7321 accaacgcca ctgcgatttg atggatttgc tcgcgcttta ccagccgagg gcgaatgtgc
     7381 cgctggacga tatggcgaaa ctgtgcggct ttccgggcaa gctgggtatg gacggcagca
     7441 aggtgtggga ggcgttccac gcgggcaggc tgaaggaaat ccgcaattat tgcgaaaccg
     7501 atgccgtgaa tacgtatttg atgtatctgc gtttctgtct ggtcagcggc agattcgacg
     7561 cggacgaata cgaaatggaa atcaagcgga tcagaaacta tctctccgcc caaacagaag
     7621 acaaaccgca ttgggcagaa tttgtccaag cgtggaaata gaaacctgct gtccgaaggc
     7681 ggtacgcttg tgccgccgtt tcggtttcag acggcgtatg gtggattaaa ttcaaaccgg
     7741 tacagccttg cctctccttg ccttactatc tgtactgtct gcggcttcgt cgccttgtcc
     7801 tgatttttgt taatccgctt taaaagctgt atccgtgccg tctgaagcct tttccagtca
     7861 aaccaaacaa tattgaaaga aaaaaatgag caaaaccgtc cgttatctga aagattacca
     7921 aacgcctgcc taccgcattc ttgaaaccga actgcatttc gacattgccg aaccgcaaac
     7981 cgtcgtgaag tcgcgtttga cggtcgagcc gcagagggcg ggcgagccgc tggtgttgga
     8041 cggttcggca aaactcttgt ccgtcaaaat caacggcgcg gcggcggatt atgtgttgga
     8101 aggcgagacg ctgacgattg cagacgtacc gtccgaacgc ttcaccgtcg aagtggaaac
     8161 cgaaatcctg ccggcggaaa acaaatcgct gatggggctg tatgcttccg gcggcaatct
     8221 gtttacccag tgcgagccgg agggcttccg caaaatcacg ttctacatcg accgtccgga
     8281 tgtgatgtcc aagttcacga ccaccatcgt cgcggacaaa aaacgctatc ccgttttgct
     8341 ttccaacggc aacaaaatcg acggcggcga gttttcagac ggccgccatt gggtgaaatg
     8401 ggaagacccg tttgccaaac cgagttatct gtttgctttg gtcgcgggcg atttggcggt
     8461 aacggaagac cgtttcacca ccatgagcgg cagaaacgtc aaaatcgagt tttacaccac
     8521 cgaagcggac aagcccaagg tcggctttgc cgtggaatcg ttgaaaaacg cgatgaagtg
     8581 ggacgaaacg cgcttcgggt tggaatatga cttggatatt ttcatggtcg tcgccgtagg
     8641 cgatttcaat atgggcgcga tggaaaacaa gggtttgaac atttttaaca ccaagttcgt
     8701 cctcgccgac agccgcaccg ccaccgatac cgatttcgaa ggcattgaat ccgtggtcgg
     8761 acacgaatat ttccacaact ggacgggcaa ccgcgtaacc tgccgcgact ggttccagct
     8821 ttcgctgaag gaagggctga ccgtgttccg cgaccaagag ttttccggcg accgcgccgg
     8881 ccgcgccgtg cgccgcatcg agaacatccg cctgctgcgc cagaaccagt tccccgaaga
     8941 cgcaggcccg accgcccatc cggtgcgccc cgtcagctat gaggagatga acaatttcta
     9001 caccatgacc gtttatgaaa aaggcgcgga agtggtgcgg atgtatcata ccctgctcgg
     9061 cgaagagggc ttccaaaaag gcatgaagct atatttccaa cgccacgacg gacaggcagt
     9121 gacctgcgac gatttccgcg cggcgatggc ggatgcgaac ggcatcaatc tcgaccagtt
     9181 cgccttgtgg tacagccagg cgggcacgcc cgttttggaa gccgaaggcc gtctgaaaaa
     9241 caatgttttc gagttaacca ttaaacaaac cgtgccgccc acgcccgata tggcggacaa
     9301 acagccgatg atgattcccg tcaaagtcgg gcttctgaac cgcaacggcg aagcggcggc
     9361 attcgattat cagggcaaac gcgcaaccga agccgtattg ctgctgaccg aagccgaaca
     9421 gatcttcctg ctcgaaggcg taaccgaagc cgtcgttccc tcgctgctgc gcgggttcag
     9481 cgcgccggtg catctgaact atccgtacag cgacgacgac ctgctgcttc tgctcgccca
     9541 tgacagcgac gccttcacgc gctgggaagc cgcccaaacg ctctaccgtc gcgccgtcgc
     9601 cgccaacctt gccgcacttt cagacggcat cgggttgccg aaacacgaaa aactgcttgc
     9661 cgccgtcgaa aaagtcattt cagacgacct cttggacaac gccttcaaag ccctgctttt
     9721 gggcgtgccg tccgaagccg aactgtggga cggcacggaa aacatcgacc cgctgcgcta
     9781 ccatcaggcg cgcgaagcct tgttggatac gcttgccgtc cgcttcctgc cgaaatggca
     9841 cgaattggac cgtcaggcgg cgaagcagga aaaccaaagt tacgaataca gccccgaaac
     9901 cgccgactgg cgcacgctgc gcaacgtctg ccgcgccttc gtcctgcgcg ccgaccccgc
     9961 gcacatcgaa actgttgccg aaaaatacgg cgaaatggcg caaaacatga cccacgaatg
    10021 gggcatcctg tccgccgtca acggcaacga aagcgatacg cgcaactgcc tgctggcgca
    10081 gtttgccgac aagttttcag acgacgcgct ggtgatggac aaatatttcg cccttatcgg
    10141 ctcaagccgc cgcagcgaca ccctgcaaca ggttcaaacc gccttgcagc atccgaaatt
    10201 cagtctcgaa aaccccaaca aagcccgttc gctcatcggc agcttcagcc gcaacgtccc
    10261 gcattttcac gcacaagacg gcagcggcta ccgcttcatc gccgacaaag tcatcgaaat
    10321 cgaccgcttc aacccgcagg tcgccgcccg cctggtgcag gcgttcaacc tctgcaacaa
    10381 gctcgagccg caccgcaaaa acttggtgaa acaagaattg cagtgcattc gggcgcagga
    10441 aggattgtcg aaagacgtgg gcgaaatcgt cggcaagatt ttgggttgag gcagtcaaac
    10501 agaaaaaaca agggtctgta ccagattagc agatatgttg ccctcgaaat at
//
LOCUS       DAWTCI010000072        10194 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWTCI010000072 DAWTCI010000000
VERSION     DAWTCI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.072"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..129)
                     /locus_tag="WM481_001731"
     CDS             complement(<1..129)
                     /locus_tag="WM481_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFA9080995.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAE"
     gene            374..1282
                     /gene="hslO"
                     /locus_tag="WM481_001732"
     CDS             374..1282
                     /gene="hslO"
                     /locus_tag="WM481_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFA9080996.1"
                     /translation="MNQTAINRADARTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRCALGELLAAGVLLSGNLKNEGTLIVQVQGQGKLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGGSIAQMLVNYMKRSEQLDT
                     HIALSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAELDAQHVLYRLFH
                     ETPPRVFEPETFESSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WM481_001733"
     CDS             complement(1368..2822)
                     /gene="mgtE"
                     /locus_tag="WM481_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFA9080997.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLAELHPADVADVLESLPPRERNIVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVCIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITIDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMVAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLSLATLFLL"
     gene            complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="WM481_001734"
     CDS             complement(3056..3808)
                     /gene="gloB"
                     /locus_tag="WM481_001734"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFA9080998.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTRFTFGNGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTVEQLYDSFQRFNQLPEGT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYR"
     gene            4040..4351
                     /locus_tag="WM481_001735"
     CDS             4040..4351
                     /locus_tag="WM481_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9080999.1"
                     /translation="MPQQTMNLMRECIPIFTVLSDENRHQILHVLWKHGRMNVNELTE
                     HLHLSRPAVSHHLKIMLQAGAVAVEQVGKERFYSIAMADAVARLKQLADLMAQNCPLS
                     K"
     gene            4425..5636
                     /locus_tag="WM481_001736"
     CDS             4425..5636
                     /locus_tag="WM481_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HFA9081000.1"
                     /translation="MLFTPFTFQNGKTAKNRIFKSAMEEQLAQNDQPSEKLVRLYGAW
                     AEGGAGVLVTGNVMVAESGKGSINDVLISDDRALEMLKKWAKARMQNDTLLIMQINHA
                     GKQSPAVVNKTPLAPSAVPLVGMNGFINPPRELSADEINGLIQQFVQTAKIAEQAGFS
                     GVQIYAVHGYLISQFLSPHHNRRQDQWGGSLENRMRFLLETYTAIRAAAGKDFLVGVK
                     LNSADFQKGGFDESESVQVVQKLSEMGIDFIEVSGGNYESPQMLAAKDSTRKREAFFI
                     DYAEKARAASQAPLIITGGFRSQTAMEDALSSGHLDLVGIARPFALVPDLANKMQNRT
                     YQTVQADRIQTGVAFVDKKAGAMLEMNWYMTQMDLIGQGKQSNPKLSAWKVLLKTLRE
                     NGKAGLSTGRA"
     gene            complement(5707..9663)
                     /gene="purL"
                     /locus_tag="WM481_001737"
     CDS             complement(5707..9663)
                     /gene="purL"
                     /locus_tag="WM481_001737"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFA9081001.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPEAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLKSET
                     FSTVDVLGGGKEALVKANTETGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIKGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDQNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVKDVRKTVTPELKNVEGSVLLFIDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDAGRLKAFYSVIQQLVAEDKLLAYHDRSDGGLFATLAEMAFAARCGISADIDCLMD
                     KFLPIHLPDFQGDPAEDLSDELYNHAAIKILFNEELGAVIQIRQKDRDYVDAAFETAG
                     LTDAVSRIGSPDFDNEFISFFGYGYFLEQNRADLQRAWQETSHAIQRLRDNPACADSE
                     FALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFTRA
                     GFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQFA
                     AFFADPNTLTLGVCNGCQMVSNLAEIIPGAETWPKFKRNLSEQFEARLNMVHVPKSAS
                     LILNEMQDSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNPNG
                     SPQGIAGITNADGRVTIMMPHPERVYRAAQMSRKPEGWTELSGWYRLFAGARKALG"
     gene            9814..10152
                     /locus_tag="WM481_001738"
     CDS             9814..10152
                     /locus_tag="WM481_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFA9081002.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
BASE COUNT         2259 a         2649 c         2965 g         2321 t
ORIGIN      
        1 ttcggcaaac tgtctgagaa tctgctcgcg gctgctgagc aggttggtca actcgtcggc
       61 aagggcgggg cggacggcaa acatcagcca caaaaccgcc caaactgccg gtttgaaaaa
      121 agaatccata tcggtgtttc cgcgcaggag gcatcgtgcc gccgtgcgat gttatgctgt
      181 atcagtcgaa aatatcttgt tgattgtata taaaaaaacg gctgtttggc aaaaaacggc
      241 ttgttagatt gagttaatat gttgattttt atataattta ttgagaattt gggaagcgca
      301 tccgccgtcc gccttgtttt cgcgccgacc gcaaccatat agccgccatc cgaacaatca
      361 gtcagagaaa atcatgaatc aaaccgccat caaccgcgcc gatgcgcgta cccgctttat
      421 cttcgacgat atgcccgtgc gcgggctgca cgtccgtctg gaaaacgtgt ggcagcacat
      481 tgtaaaacag aaaaactatc ccgccgccat ccgctgcgcc ttgggagagc tactggctgc
      541 cggcgttctg ctgtcgggca accttaaaaa cgaaggcacg ctgattgtgc aggttcaggg
      601 gcagggaaaa ctgaaaatgc tggttgcgga agcgacttcc gaccgtaccg tccgtgcaac
      661 cgcgcggtgg gacgaaaccg cagaaatagc cgatgacgaa agcctcggcg accttttggg
      721 cggcaacggc gtgttcgtat tgacgctgca acccaaagac ggcgaaccct ggcagggcgt
      781 agtgcctttg gaaggcggca gcatcgcgca aatgttggtg aactatatga aacgttccga
      841 acagcttgat acgcacatcg ccctgtctgc aagcgatgaa gcggcgggcg ggctgctggt
      901 gcagcgtctg cctgaagagg tgttggatga agaggcatgg gaacacgtca gtacgctggc
      961 gcgcacgctg acggcggagg agctggcaga attggacgcg caacacgttt tataccgcct
     1021 gttccacgaa acgccgccgc gcgtgttcga gccggaaacg tttgaatcct catgtacctg
     1081 ttctcggggc aaggtcagcg atatgctgtt gatgctgggc ggggaagaag tcggcggcgt
     1141 ggtggcggaa caaggcagca tcgaagtcga ttgcgacttc tgccacagca aatacgtgtt
     1201 tgatgaaacc gatgtcaacg cgctgttcgg ggaggatgtg gtcggcgttg ccaaagggct
     1261 gccccgacat accgtccaat aatcttgagc aacaaggatg aatggtcgaa tgccgtctga
     1321 agcagcttct gcttcagacg gcatttttgc gcgggtcggg acggcattta aagcaggaac
     1381 agcgtggcga gactcaagaa aatcaggaag ccgccggagt cggtaacggc ggtaatcagc
     1441 accgagctgc ccagcgcggg atcgcgtccg aatttttcca ttaccacggg aatcaatacg
     1501 ccgaccgttg ccgccagcag gaggttgagc gtcatcgcgg caaccatgac caaaccgatg
     1561 ccgaggctgc cgtaaagcag ccacgaaacc gctcccatca ccgtccccca aataatgccg
     1621 ttgaccaagg cgacgccgac ttcttttttc aacagccttc ccgcctgcat atccgtcagc
     1681 tgccccatcg ccatcgcgcg gacaatcatg gtaatcgtct ggttgcccga gttaccgccg
     1741 atgccggcga cgatgggcat cagcgcggcg agtgcgacga ttttttcgat actgccttca
     1801 aacgcgccga tgacgcggct ggcgaggaag gcggtgcaga ggttgacggc gagccacatc
     1861 cagcggtttt tgacggaatc caagacgggg gcgaacaggt cttcctcttc ctgcaaacct
     1921 gccatgttca acatatccgc ttcggattct tcgcggatca cgtccaccat ctcgtcgatg
     1981 gtaatcctgc cgatgagctt tttgttttca tcaacgacgg gcgcggtaac caagtcgtag
     2041 cgttcaaacg cctgcgccgc ttcttccacg tcgtcctcgg cgcggaaacg cacgacatct
     2101 ttcgccatca cgttttccac caaatcttcg ggatcggcga ccaaaagttt gcggatgggc
     2161 agcacgccct gcagcacgtc gttttcatcg acgacaaaaa tcttgtcggt atggtcgggc
     2221 aggctgtcga agcggcgcag ataacgcagc accacttcgc aggcgacatc ggcgcggatg
     2281 cagaccaact cgaagtccat aatcgcaccg acttggttgt cttcatacga cattgccgcc
     2341 ttgacttggg cgcgctcttc ctcatcgcgc gtctgtaagg cttcataaac cacttggtgc
     2401 ggcaaatcgt ctgccagctc cgccaattcg tccgcatcca aatcatcgac cgccgccaac
     2461 aattcgtcct tgtccattga ctcgatcagc gtttctcgca ccgcgtcgga tacttccagc
     2521 aatacttcgc cgtcgtcttc gggtttgacc agaagccaaa cgatattgcg ttcgcgcggc
     2581 ggcagggact ccaagacgtc ggccacgtcg gcagggtgca actcggccaa gaggacggtc
     2641 agctcggtca gtttgtcgcg cagcggcgca tcttcgagcg gtgtgccgtt ttcgatttgt
     2701 tcaaaagcag gttcgaggat ttcgcagagg gagtggacac ggtcgaaatc ggcggaaggg
     2761 cgttcggcat ggttttgacc ggtttcgccg tcgtgtgtcg gaggagtgtg ttcgatgctc
     2821 ataaatgctc cgcccgccgt gtgcggggag gcattccggc gggatggtta ttgtcgtttg
     2881 gaatcggaag gggtgttcag taaaaacgaa ctgggaaggt ccataataaa agcccgaaaa
     2941 atcaggcacg ggattggaaa cagcactatt ctactccctt ttgaaacggt ttactatttt
     3001 taacgcaaat gccgtctgaa acgcgggttt cagacggcat ttttcggagg gcaggtcagc
     3061 ggtaggcgtt tttcagttcg cgcagggcgg cgaatacttc caaaccgctg ttcagcgttt
     3121 tgccgaccag ggcttcggca cgttggcgga cggcggggat ttctgtccgt aagaacgggt
     3181 tgacgcggcg ttcgtgcgcg agggtaacgg gcagggtggg cgtgcgttcc gccgccttca
     3241 atgccgtctg aatgtcggcg ttgtccggct cgatatgggc ggcgaaacgc aggttggcgg
     3301 cggtgtattc gtgtgccgga tagaacaggg tgccttcggg taattggttg aaccgttgga
     3361 agctgtcgta aagctgttcg accgtgccgg taaacacgcg tccgcagccg gcggaaaaaa
     3421 gggtgtcgcc gcagaaaacg tgtatgccgt ctgaagtttc gagaaggtag ctggtgtggc
     3481 ggtctgtgtg gccgggtgtt gcccaaacgg taacctgacc gttgccgaag gtgaatcgtg
     3541 tgccggcggt tacggtgtgg gtggcggctt cgatgtcgga ttcgccgtaa acgggcgatt
     3601 ccatgtagcc gcgccagagt gccgccgcac cgccttcgtg gtcgggatgg gggtgcgtta
     3661 cccaggtttg ggcgagcatg agtcggttgc gtacgaggaa ttccaagacg ggcgagggtt
     3721 cggaaggatc gacgcagacg gcatggttgc cgtgctgtat catccagatg tagttgtcgg
     3781 tcagggcttt gacgggggta attttcatga gtgttccttg atggttcgga tgccgtctga
     3841 aaggtgtatc cgaaaggcgt taatatacag cggaacccta gcaaacgcca tcatgctgtt
     3901 tcaggcggca ttttttctta taaaaacctt tcagcaacaa tatattgcca aactgcaaac
     3961 caaactgcct ttgataactg ccgaccttcc ctctaaaata cgttcgaatt tttaaatata
     4021 taaactcaat agatcgaata tgccgcaaca aaccatgaat ttaatgcgcg agtgcatacc
     4081 catttttacc gtattgagcg atgaaaaccg ccatcagatc ctgcatgtat tgtggaaaca
     4141 cggtaggatg aatgtgaatg agctgaccga gcatctgcat ctgtcgcgcc ctgctgtgtc
     4201 gcaccattta aaaatcatgc tgcaagccgg agcggtggcg gtggagcagg tcggcaaaga
     4261 gcggttttac agtattgcca tggcggatgc ggtggcgaga ttgaaacagc ttgccgattt
     4321 gatggctcaa aattgcccgc tttcaaaata gggttatccg ttggcccttt atttttaccc
     4381 aaatacgttt acatttttaa acttatcaac ttaaaggaac gatcatgtta ttcacgccat
     4441 tcacttttca aaacggcaaa accgccaaaa accgtatctt caaatccgcc atggaagagc
     4501 aacttgccca aaacgaccag ccgtctgaaa aacttgtccg cctgtacggc gcttgggcag
     4561 aaggcggcgc aggcgttttg gtgacgggca atgtgatggt ggctgaaagc ggcaaaggtt
     4621 cgataaacga tgtgttgatt tcagacgacc gcgcccttga aatgctgaaa aaatgggcga
     4681 aagcccgcat gcaaaacgac acgctgctca tcatgcaaat caatcatgcg ggcaaacagt
     4741 cgcctgcggt ggtcaataaa acaccgcttg caccgagcgc cgtgccgctg gtgggcatga
     4801 atggctttat caatccgccg cgcgaattga gtgccgatga aatcaacggg ctgattcagc
     4861 aattcgtaca aactgccaaa attgccgaac aggcaggctt ttcaggcgta caaatttacg
     4921 ccgtacacgg ttatctcatc agccaattcc tctcgccgca ccacaaccgc cgccaagacc
     4981 aatggggcgg cagtttggaa aaccgtatgc gcttcctttt ggaaacctac accgctattc
     5041 gcgctgccgc gggcaaagat ttcttggtgg gcgtaaaact caattcggca gatttccaaa
     5101 aaggcggatt tgacgaaagc gaatcggtgc aggtggtgca aaaactgtcg gaaatgggca
     5161 ttgattttat tgaagtttcc ggtggcaact acgaaagccc gcaaatgctc gccgccaaag
     5221 acagcacccg caaacgcgaa gccttcttca tcgattacgc cgaaaaagcc cgtgcagcca
     5281 gccaagcccc gctgattatc accggcggat tccgttcgca aaccgccatg gaagatgcct
     5341 tatccagcgg tcatttggat ttggtcggca tagcccgccc gtttgcctta gtccctgatt
     5401 tggcgaacaa aatgcaaaac agaacttacc aaaccgtaca agcagaccgc atccaaactg
     5461 gcgtagcatt tgttgataaa aaagcgggtg caatgttaga aatgaactgg tatatgacgc
     5521 aaatggattt gatcggacaa ggaaaacagt ccaaccctaa attgtcggcg tggaaagtat
     5581 tgctgaaaac tttgcgggaa aacggtaaag cgggattgag taccggcagg gcttgagaat
     5641 ggtcaaaatg ccgtctgaaa cgggtttcaa aacctttttc agacggcatt tgtttgggca
     5701 gacggtttag cccaaggctt tacgtgcgcc ggcaaagagg cggtaccagc cggacagttc
     5761 cgtccagcct tccggtttcc ggctcatttg tgcggcacgg tacacgcgtt cggggtgggg
     5821 catcataatg gtaacgcggc cgtcggcgtt ggtaatgccg gcgatgccct gcggcgagcc
     5881 gttggggttg agcggatagg tttgggtaac ctggttttga ccgtccacat attgcagcgc
     5941 gatgcccaaa tcggcggaaa tattgccgcc gtgaagcgcg aagtcggcgc ggccttcgcc
     6001 gtggctgacc acgacaggca ggctggagtc ttgcatttcg ttcaaaatca gcgacgctga
     6061 tttggggacg tgaaccatat tcaggcgcgc ttcaaactgc tcgctcaggt tgcgtttgaa
     6121 cttcggccat gtttctgcgc ccgggataat ttcggcaagg ttgctgacca tttggcagcc
     6181 gttgcacacg cccaatgtca gcgtgttcgg atcggcaaag aaggcggcga attggtcgcg
     6241 caaggcaggg tggaacagga tggatttcgc ccagccttca cccgcgccga gtacgtcgcc
     6301 gtagctgaag ccgccgcacg ccgccagcat tttgaagtcg gcaaggcgga agcggccagc
     6361 catcaggtcg gacatatgca cgtcgtaggc atcgaatccg gcgcgggtga aggcggcggc
     6421 catttcgatt tgcccgttta cgccctgttc gcgcaggatg gcgattttag gtttcgcgcc
     6481 actgttgata aacggcgcgg cgatgtcttc gttcacgtcg aacttcacgt cggcaaacaa
     6541 tgcgctgcgt tcgttgtcgc caatcagggc gaactcgctg tcggcgcagg cagggttgtc
     6601 gcgcaggcgt tggatggcgt ggctggtttc ctgccacgcg cgttgcaggt cggcgcggtt
     6661 ttgttccaag aaataaccgt aaccaaagaa agaaataaac tcattgtcaa aatcaggaga
     6721 gccaatccgg ctgaccgcat cggttaagcc ggccgtttca aatgccgcat caacataatc
     6781 cctatctttt tggcggattt ggataaccgc gcctaattct tcattgaata agattttaat
     6841 ggcggcatga ttataaagtt cgtcagataa gtcttcggca gggtcgcctt gaaaatccgg
     6901 gagatgaatc ggcaggaatt tatccatcag gcaatctata tcggcactaa taccgcaccg
     6961 tgccgcaaac gccatttccg ccagcgtcgc aaacaaaccg ccgtcgctgc ggtcgtgata
     7021 cgccaaaagt ttgtcttcgg cgacaagctg ttgaattacg ctgtaaaacg ctttcagacg
     7081 gcctgcatcg tccaaatcgg gcgcgtcgcc gctcatgttg ttgtacacct gaccgaacgc
     7141 cgagccgccc atgcgcgctt tgccgaagcc caaatcgata aacagcaata cgctgccttc
     7201 gacgtttttc aattcgggcg taacggtttt tcgcacgtct ttaacaggcg cgaacgcgga
     7261 aataatcagg ctcaacggcg aaaccacgga ttttttctct tcgccgtcct gccacacggt
     7321 tttcatcgac aggctgtctt tacctacggg aatgctcaaa tccaatgcct ggcaggcttt
     7381 agaaacggct tcgacggtgc ggtagagttt ttcgtcttcg ccttcgttgc cgcacgccgc
     7441 catccagttg gcggaaagtt tgatgttgcc gatgtcgccg atgttgactg ccgcgatgtt
     7501 ggtgatggct tcgccgacgc acattctgcc cgaggcgggc gcgtcaaaca gggcgacggc
     7561 cggtttttcg cccatagaca tcgcttcgcc gcgataggtg ttgaagccca tcatggtaac
     7621 ggcgcagtcg gctacggggg tttggtattt gccgaccatt tggtcgcggt gggtcatgcc
     7681 gccgacgctg cggtcgccga tggtaatcag gaagtttttg gcggctacgg caggcaggcg
     7741 caaaacgcgg taggcggctt cggtgatgtc gatatcgccc gcgtgaaacg gttttttgga
     7801 cggcgtaacc gttttgtcgg tacgcgtggt tttgggcggt ttgccgagca agacgttcag
     7861 cggcaaatcg acggggttgt tggagaacaa atcgtcgcgc actttcaaat gaccgtcgtc
     7921 ggtcgccgtg ccgactacgg caaacgggca gcgttcgcgt tcgcagatgg cgcggaaggt
     7981 gtccaaatct ttttccaaaa tcgacaagac ataacgctct tgcgattcgt tacaccagat
     8041 ttgcaaaggc gttaaaccgt gttcttccaa cggcacttcg cgcagctcga ataccgcgcc
     8101 gcgtccggca tcgttgacca attcagggaa agcgttggac aagccgcccg cgcccacgtc
     8161 gtggatggag ataatcgggt tttggtcgcc gagctgccag cagcggtcga tgacttcctg
     8221 cgcgcggcgt tcgatttcag ggttgccgcg ttgtacggag ttgaagtcca aagacgcatc
     8281 atttgtaccg gtatccatcg aagaagccgc accgccgccc aagccgatga gcatgcccgg
     8341 gccgcccagt tggatcagca gtgcgccttc ggggatttcg tctttgtgcg tctgctgcgc
     8401 ctgaatgctg cccaaaccgc cggcaatcat aatcggtttg tggtagccgc gaacctgacc
     8461 gtcaaatttt tcttcaaagg tgcggaagta gcccaagagg ttcgggcggc cgaattcgtt
     8521 gttgaacgcc gcaccgccaa tcgggccttc aatcatgatg tccaacggcg aggaaatgtg
     8581 ttccggcttg ccgtagtctt gttcccacgg ctgtttgagg tcggggatgt tgaggttgga
     8641 cacggtaaag ccggtcaagc ccgctttcgg acgcgaacct ttgcccgtcg caccttcgtc
     8701 gcggatttcg ccgcccgcgc ctgttgccgc gcccgcaaac ggcgcgatgg cggtcgggtg
     8761 gttgtgcgtt tccactttca tgatgatgtg ggtgtcttcc tcgtggaaac ggtagccttg
     8821 gttttccgct gcattcggat agaaacgctc gattttcgcg cctttgatca cggacgaatt
     8881 gtctttatag gcgacgaccg taccttcggg atgcgcgttg tgcgtgtcgc gtatcatgcc
     8941 gaacagggat ttcggttgtt tttcgccgtt gaggatgaaa tcggcgttga agattttgtg
     9001 gcggcagtgt tcgctgtttg cctgcgcgaa catcatcagc tccacatcgg acggattgcg
     9061 ctgcaaagcc tgatagtttt caaccagata atcgatttcg tcggcggaga gtgccaaacc
     9121 cgtttcggta ttggctttga ccaaagcctc tttaccgccg cccaaaacat cgacggtgga
     9181 gaaggtttcg gatttgagat ggtggaataa tttggatgcc gtctgaaaat cgggcagcac
     9241 ggattcggtc atgcggtcgt gcagcaacgc cgcccattgc tgtttctgtt catcggtaag
     9301 cgcaccttcc agccacaccg ccataccgcg ctcgatgcgt tcgatgcctg ccaaaccgca
     9361 gttttccgcg atattggtcg ccttggaagc ccacggcgaa atcgtaccca aacggggcgt
     9421 taccaaaaac aaatgcaaac cctcgcgcgc ttcgggcgtt tgttcaacgc tttgcgccgc
     9481 caacaaggct tgcagttttt cgacagtcgc ggcatcaagt gctttctcgc tgccggcgaa
     9541 ataccaaaat tcgctgctta atttgacttc gggcaggccg agtgcggcgg ctttttgcaa
     9601 gagtttttcg acacggaaat cggaaagggc ggtaacgccg cgcaagggca aaacaacaga
     9661 catggattcg gctctcaaat gcggttggaa aagctgtcat tatatgcgca acgggggctg
     9721 tattgcttgg gtattttggt ttaaaatgtt gacaatctga cgggggttcc cgtttttcgg
     9781 atgtgtttgt attgtttgtt caaaggaaat tttatgaaaa aaatcgaggc gattgtcaaa
     9841 ccgttcaagc tcgacgacgt gcgcgaggcg ttgacggaaa tcggcattac gggcatgacc
     9901 gtcagcgagg tcaaagggtt cggcaggcag aaggggcata cggaaatcta tcgcggtgcg
     9961 gaatacgccg tcgatttcct gcccaaggtc aaaatcgagt tggtgttggc ggatgatgcc
    10021 gtggaacgcg cgattgacgt gattgtcgag gtggcgcgtt cgggcaaaat cggcgacggc
    10081 aagatttttg tgctgccggt cgaggaggca atccgtatcc gcacgggcga acgttcggac
    10141 gcggcggttt gagcgtttgg aatggaaatg ccgtctgaaa ggctttcaga cggc
//
LOCUS       DAWTCI010000073         9914 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWTCI010000073 DAWTCI010000000
VERSION     DAWTCI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.073"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            446..1009
                     /locus_tag="WM481_001739"
     CDS             446..1009
                     /locus_tag="WM481_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFA9081003.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            1012..1560
                     /locus_tag="WM481_001740"
     CDS             1012..1560
                     /locus_tag="WM481_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081004.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQEFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            1567..4782
                     /gene="carB"
                     /locus_tag="WM481_001741"
     CDS             1567..4782
                     /gene="carB"
                     /locus_tag="WM481_001741"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFA9081005.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKADNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGRTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSEDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLMKEEKSVSDGQLQDLDYAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAIKEAVFPFIKFPGVDTILSPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQAINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDYLGVYSVQELHGRLKNRN"
     gene            complement(4983..6023)
                     /gene="queA"
                     /locus_tag="WM481_001742"
     CDS             complement(4983..6023)
                     /gene="queA"
                     /locus_tag="WM481_001742"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFA9081006.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETVAAVEAAKARGNKAWAVGTTSMRALESAARATGYLKDGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVGAFSGMGHIRAVYRHAIEREYRFFSYGD
                     AMVLGRNEGGGL"
     gene            6140..6397
                     /locus_tag="WM481_001743"
     CDS             6140..6397
                     /locus_tag="WM481_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFA9081007.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENEHNFTVVSHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6379..6840
                     /locus_tag="WM481_001744"
     CDS             6379..6840
                     /locus_tag="WM481_001744"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFA9081008.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            6954..8315
                     /gene="accC"
                     /locus_tag="WM481_001745"
     CDS             6954..8315
                     /gene="accC"
                     /locus_tag="WM481_001745"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFA9081009.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKGSLHVKL
                     ADESVCIGPAASAQSYLNIPAIIAAAEVTFADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDDAEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVLADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFIDEEARKKIGKACTDACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQEGGVSIHYLEHWLEARKTK
                     QDK"
     gene            8419..9306
                     /gene="prmA"
                     /locus_tag="WM481_001746"
     CDS             8419..9306
                     /gene="prmA"
                     /locus_tag="WM481_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFA9081010.1"
                     /translation="MPYQQITVNVNDAVAERLADVLMEHGALSAAIEDACAGTQNEQA
                     IFGEPGMPTEQIWQQSKVIALFGEHDEAAAVIDAAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGCAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGRDNAEQNNVDAQFFLP
                     DSLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETDEGWARLSGVKR"
     gene            9324..9887
                     /gene="orn"
                     /locus_tag="WM481_001747"
     CDS             9324..9887
                     /gene="orn"
                     /locus_tag="WM481_001747"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFA9081011.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDDLLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWIPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPIAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPKAEAQ"
BASE COUNT         2540 a         2810 c         2521 g         2043 t
ORIGIN      
        1 gagagggcaa caagccgcaa ggcttgtatt tgggcggcga aggtattagg aaagattgcc
       61 accattcgga gaatgccctc cccccagccc tcccccacgg gggagggagt gggtttcggc
      121 agatttttcg gttgccggtg gtttgaaaaa taacttaaat ttgcaaccgt tgatttcagg
      181 tcgtctgaaa aaggatgcac gaaatatcaa cagcgggaat ttttcaggta gcctttattg
      241 tcaagcagat ggaacaaacg ccgcgagcgt tttttcagac aatctttgaa ctcatcggta
      301 gagtgtgtgc cgcaaggcgc gcatgcggtg ggtcggggtt acagggaaaa tgaagaacgc
      361 gtgcgtgcgt accgcacacc ctacatgcgg gctacggctt gctaggattg ctcgttatac
      421 ttaattttga caaggagtca atgaaatggc gatagaacta aatggtaact ggacaaaagg
      481 atttgcttta gaccatcata tgcaaaaaag tattttgtta ggttatgatg aattagggca
      541 tcctcacttt gatagcaaga gaagtgtgat tggagaatgg gtttatcaat taaaatatcg
      601 taatcaaacc caaaatgttt ctttgctggt agactatatt ttacaaaaat ttagtggttt
      661 agaatctata aatttaattg ttcccgcccc ttttacaaca gagcgtatta atcaaccagt
      721 tcaattaatt gccaaagaac taagtgatcg cttgaatatt ccatattctc ctattttaag
      781 aaaatctagc tcacacacac cactcaaaaa tattgaagaa aaatcagaaa aactagaaat
      841 actgaataat tccatcacaa ttgacaatat agatctatcc aataagaata ttttagtaat
      901 cgatgattta tttgatagcg gagcaacatt ggaaatatca actgaaaaat tgttttcaaa
      961 aaatgctaga tctgtaattg ttttagcaat gacaaagact agaggataaa aatgcgcact
     1021 attttctttt ctggttcacg cagcatttca cgactaaatc cacaaatacg agaaagaatt
     1081 aacaatattc ttagcaataa ttttgatatt gttattggag atgctaatgg ggctgataaa
     1141 gctatccaag aatttctaca agaacaagat tatgctaatg tgcatattta cttttctggc
     1201 aaaatttacc gaaataatgt gggaaattgg cagtttgtcc aagtagacag caaaggaact
     1261 ggaagggtat tttatacagc aaaagataag aaaatggcag aaattgctga ttatggcttt
     1321 attttatggg atggtaagag tattggttca ttaaataata tagcagaact tttacagcta
     1381 aataaacctt ctcttgtata ccattctcaa acaaaagaat tctttaaaat taaatcaagc
     1441 gcagatttag agaatatttt atctaacata gaagatgatg ttttagcttc aattttagaa
     1501 aagggaaata cttttcttaa aagctatgta acaaaacaac cttctttaat tcaagagtaa
     1561 ccacccatgc ccaaacgtac cgacctaaaa tccatcctta tcatcggcgc cggccctatc
     1621 gttatcggtc aggcctgcga atttgactat tcgggcgcac aggcctgcaa agccttgcgt
     1681 gaagaaggct ataaagtcat tttggtgaat tccaaccccg ccacgattat gaccgacccc
     1741 gaaatggcgg atgttaccta catcgagccg attatgtggc agacggtgga gaagattatc
     1801 gccaaggagc ggcccgatgc gattctgccc acgatgggcg gtcagaccgc gctgaactgt
     1861 gcgctggatt tggcgcgtaa cggcgtgctg gcgaaataca atgtcgagtt aatcggcgca
     1921 acggaagacg cgatcgacaa ggcggaagac cgcggccgct ttaaagaagc gatggaaaaa
     1981 atcggcctct cttgcccgaa atcttttgtc tgccacacca tgaacgaagc cttggcggcg
     2041 caagaacagg tcggctttcc gacgctgatt cgtccgtctt tcacgatggg cggttcgggc
     2101 ggcggcattg cctacaataa ggatgagttt ttggcgattt gcgaacgcgg tttcgatgcg
     2161 tcgcctacgc atgagctgct gattgagcag tctgtgctcg gctggaaaga gtacgagatg
     2221 gaagtggtgc gcgataaggc ggacaactgc atcatcatct gttcgattga aaacttcgac
     2281 ccgatgggcg ttcatacggg cgactcgatt acggttgcgc cggcgcaaac gctgacggac
     2341 aaggaatacc aaatcatgcg caacgcttcg ttggcggtat tgcgcgaaat cggcgtggac
     2401 acgggcggct cgaacgtgca gtttgcggtg aaccctgaaa acggcgagat gattgtgatc
     2461 gagatgaacc cgcgcgtgag ccgttcgtcc gcgctggctt ccaaagcaac gggcttcccg
     2521 attgcgaagg tggcggcgaa gctggcggtc ggctttacgc tggacgagtt gcgcaacgac
     2581 atcaccggcg gccgcacgcc cgcgtcgttc gagccttcca tcgactatgt ggtaaccaaa
     2641 atcccgcgtt tcgcgtttga aaaattcccc gccgcagacg accgcctgac cacgcagatg
     2701 aaatctgtgg gcgaagtaat ggcgatgggc cgtaccattc aggaaagctt ccaaaaagcc
     2761 ttgcgcggtt tggaaaccgg cttgtgcggt ttcaatccgc gcagcgaaga caaagcggaa
     2821 atccgccgcg aactggccaa ccccggcccc gaacgtatgc tgtttgtggc agacgcgttc
     2881 cgcgcgggct tcacgccgga agaaatccac gagatctgcg ccatcgaccc ttggttcttg
     2941 gcgcaaatcg aagacttgat gaaggaagaa aaatcggttt cagacggcca gttgcaagat
     3001 ttggattacg ccgccctacg ccgtctgaaa cgcaaaggct tctccgacaa acgtttggca
     3061 caattgttga acgtaagcga aaaagaagtt cgcgaacacc gctacgcgct gaagctgcat
     3121 ccggtttaca aacgcgtcga tacctgcgcc gccgagttcg ccaccgaaac cgcctatctt
     3181 tactccactt acgaagaaga atgcgaatcc cgtccttccg accgtaaaaa agtgatgatt
     3241 ctcggcggcg gcccgaaccg catcggtcag ggcatcgagt ttgactactg ctgcgttcac
     3301 gccgcactcg ccctgcgcga atcgggcttt gaaacgatta tggtcaactg caaccccgaa
     3361 accgtgtcca ccgacttcga caccagcgac cgcctctatt tcgagccgct gacattggaa
     3421 gacgtgttgg aaatcgtccg caccgaaaac ccgtggggcg tgattgttca ttacggcggt
     3481 caaaccccgc tgaaactcgc caacgcattg gttgaaaacg gcgtgaacat catcggcaca
     3541 tccgccgaca gcatcgatgc cgccgaagac cgcgaacgct tccaaaaagt gttgaacgac
     3601 ttaggcctgc gccaaccgcc caaccgcatc gcccacaacg aagaagaagc actcgtcaaa
     3661 gccgaagaaa tcggttatcc gctggtcgtg cgcccgtctt atgttttggg cggacgcgcg
     3721 atgcagattg tccactccgc cgaagccttg caaaaataca tgcgcgaagc cgtgcaggtt
     3781 tccgaagaca gccccgtgct gctcgatttc ttcctcaaca acgcgattga agtcgatgtg
     3841 gactgcgttt cagacggcaa agatgtggtt atcggcggca tcatgcagca cgtcgaacag
     3901 gcgggcatcc actccggcga ctccggctgc tcgctgccgc catactcctt aagcgaagaa
     3961 atccaagacg aaatccgccg ccaaaccaaa gcgatggcgt acgcgctggg cgtggtcgga
     4021 ctgatgaacg tgcagtttgc cgtgcaggac ggcgtggtgt tcgtgttgga agtgaacccg
     4081 cgcgccagcc gtaccgtccc ctttgtctcc aaagccaccg gcgtgccgct cgccaaagtc
     4141 ggcgcgcgct gcatggcagg catttccctg aaagaacaag gcgtggaaaa agaagtcgtc
     4201 cccgatttct atgccattaa agaagccgtg ttcccattca tcaaattccc gggcgtggat
     4261 acgattttga gtccggaaat gcgctccacc ggcgaagtga tgggcgtggg cgcaagtttc
     4321 ggcgaagcct actacaaagc ccaactcggc gcgggcgaac gcctcaaccc gaccggcaaa
     4381 atcttcctct ccgtgcgcga agaagacaaa gaacgcgtca ttaaaaccgc caaaaacttc
     4441 caagccttag gctacggcat ctgcgccacg cgcggcacgg cgcaatacct gaccgaacac
     4501 gggctgattg tgcaggccat caacaaagtc cccgaaggcc gcccgcacat cggcgacgcg
     4561 ctgaaaaacg gcgaaatcgc actggtcgta aataccgttt ccagcgatcc gcaatccgtg
     4621 tccgacagcc acatcatccg ccaaagcgca ttgcagcaac gcgtgccgca atacaccacc
     4681 accgccggcg gcgaagcgat gagcgaaggc gcgaaaagcc gggattatct gggtgtgtac
     4741 agcgttcaag agctgcacgg tcgtttgaaa aaccgcaact gatgcctgaa tcaggttgaa
     4801 caatgccgtc tgaagccgtt ttgcggtttc agacggcatt ttgttgcaca tagccttcac
     4861 tttcttttga cctttaagca ccctaatttg gggcggtgga ttatccaact gccggacatc
     4921 cataaaaata ccgcccgaac cacggttcag acggcatctg ccttgcaacg gacagcagca
     4981 gtttaaagcc cgcccccttc gttccgcccc aaaaccatcg catctccgta gctgaagaaa
     5041 cggtattcac gttcaatcgc atgacggtac acggcgcgga tatgacccat acccgaaaac
     5101 gcgccgacca acatcagcag cgtcgatttc ggcagatgaa aattggtaac cagcctgtcg
     5161 acaacattaa aacggtagcc cggcgtgatg aaaatatcgg tgtcgccctg tccgtctttc
     5221 aaatatcccg ttgcgcgcgc ggcagactcg agggcgcgca tggaagtcgt gccgaccgcc
     5281 caggctttgt tcccccgggc ttttgccgcc tcaacggcgg cgacggtttc agacggcact
     5341 tcaaaccatt cgctgtgcat tttgtgttct tcgattttat cgacgcgcac gggttggaat
     5401 gtccccgcac cgacgtgcag ggttacttct gcggttaccg cgcctttgtc tttcagacgg
     5461 cgcaaaagtt cttccgtaaa atgcaggccc gccgtcggcg cggcgaccgc gccctgatat
     5521 ttggcataaa cagtttgata acggctgtcg tcgtccgcat cggcggcacg ttcgatataa
     5581 ggcggcaggg gcaggtgtcc gttctgttcc aaaagttcgt aaacggtttg accgccttca
     5641 aaacgcaggc agaacagttc gccctcacgt ccgaccatca cggcacggat accgccttca
     5701 aacaccagcc ccataccggg cttgggggac ttggacgaac ggatgtgcgc caatgcggta
     5761 tggttgtcca acacacgctc aatcagggct tcgatcctgc cgccgctgtc tttctgccca
     5821 aacagccgcg ccttcatgac tttggtgttg ttgaatacca aaacgtcgcc tgcctcgaca
     5881 taatcgggca aatcgccaaa cacccggtct tgcagcggca tatcgggcag cgcgaccaaa
     5941 agccggctgc tgccgcgcac ctcgggcgga tgctgggcaa tcaggtgttc gggcagggta
     6001 aagtcaaaat ctgaaatatc catttttaca ctctcgttcg ggcaagccgc cattatacgc
     6061 actttagccc tttttcagac ggcatctttg cccgaaaaac caacagatta gaataaacac
     6121 tcttgactcg gggcatcttg tgcgcaaaat caaacttcct gcacatttcc cccaaaaacc
     6181 gccgtttttt gatattttac tggacattta ccgacaactt cgggaaaatg aacacaattt
     6241 cacggtcgtt tcccaccaca ggaaaaccgt atccgaacac cattccgccc ggtttgcgcc
     6301 gttgccgcaa gccggctgtt ttctgaaaaa ccaacgcaac aacccgccgg aacaccggca
     6361 gcctttaaag gaacagaaat ggatttgcgc aaattaaaaa aactgattga tttggttgaa
     6421 gaatcgggta tcgccgaaat cgaagtaacc gaaggcgagg aaaaagtccg cattacccgt
     6481 accattgcag ctgcggcagc tcccgtttac gccgcgcccg tacctgccgc cgcgccggcc
     6541 gtaacgccgg ccgccgcacc cgttgcggca tccgcgcccg ccgccgcgcc ggccgcccgc
     6601 gatttgtccg acgctcaaaa atcgcctatg gtcggcacgt tctaccgcgc gcccggcccg
     6661 aatgccgccg catttgtcga agtcggtcaa caggtcaaag ccggcgacac gctgtgcatc
     6721 atcgaagcga tgaagctgat gaacgaaatc gaagccgaaa aatccggcac ggtcaaagaa
     6781 attctggtcg aaaacggtac gcccgtcgaa ttcggcgaac cgctcttcat catcggataa
     6841 tcctgttttc agacggcata aacttccgat gccgtctgaa atgctttccc ccccttcagc
     6901 gttcccgcac ccttttttac ggacgggttg ccggaaccgc aggaaaggtc atcatgctga
     6961 aaaaagtttt aatcgccaac cggggcgaaa tcgccttgcg cgtactccgt gcctgccgcg
     7021 aaatgggcat tgccaccgtt gccgtgcatt ccgaagccga caaaggcagc ctgcacgtca
     7081 aactcgccga cgaatccgtg tgcatcggtc ctgccgcttc tgcgcaaagt tatctgaaca
     7141 ttcccgccat cattgccgcc gccgaagtaa ccttcgcgga cgctgtccac ccgggctacg
     7201 gcttccttgc cgaaaacgcc gatttcgccg aacaggtcga acagtccggc tttaccttca
     7261 tcggcccgaa acccgacacc atccgcctga tgggcgacaa agtctccgcc aaacacgcga
     7321 tgatagcggc aggcgtgccc tgcgtccccg gctctgacgg cgcattgccc gacgatgatg
     7381 ccgaaatcct caaaatcgcc gacaaagtcg gttatcccgt gattatcaaa gcatcgggcg
     7441 gcggcggcgg gcgcggtatg cgcgtggtcg agaaaaaaga agacctcctc cagtctgtcg
     7501 aaatgaccaa ggctgaagcg ggcgcggcgt tcggcaaccc gatggtttat atggaacgct
     7561 acctgcaacg cccgcgccac gtcgaaatcc aagtgcttgc cgacgaacac ggcaacgccg
     7621 tctatcttgc cgagcgcgac tgttcgctgc aacgccgcca ccagaaagtc atcgaggaag
     7681 cacccgcccc gtttatcgat gaagaggcac gcaaaaaaat cggcaaagcc tgtaccgacg
     7741 cgtgcaaacg catcggctac cggggcgcgg gcacgtttga atttttatac gaagacggcg
     7801 aatttttctt tatcgagatg aacacgcgcg ttcaggtcga gcatccggtt accgaactca
     7861 ttaccggcgt ggacatcgtc caagagcaac tccgcatcgc atccggcctg ccgctgcaat
     7921 acaaacagaa agacatcaaa atcgaaggcc acgccttcga gtgccgcatc aatgccgaag
     7981 acccgtacaa cttcatcccc agcccgggcc cgattgaaag ctgccacctg cccggcggct
     8041 tcggcatccg cgtggacagc cacatctacc aaggctaccg catcccgccg tactacgaca
     8101 gcctgatcgg caaaatctgc gtacacggca aaacgcgtga acaggcaatg gcgaaaatgc
     8161 gcgtcgcact cgccgagctg gcggtaaccg gcatcaaaac caatacgccg ttgcaccgcg
     8221 acctgttcgc cgatgcgggt ttccaagaag gcggagtgag catccattac ctggagcact
     8281 ggctggaagc gcgcaaaacc aaacaggaca aataagccgc cgccgatatg ccgtctgaag
     8341 ccgcccttcc gcgttcagac ggcatttccc ttgccccgcc gtctgaaacc gatttcgata
     8401 tttccaagaa agcccgttat gccctaccaa caaatcaccg tcaacgtcaa cgatgccgtc
     8461 gccgaacgcc tcgccgacgt gctgatggaa cacggtgcac tctccgccgc catcgaagat
     8521 gcctgcgccg gcacgcaaaa cgaacaggcg attttcggcg aacccggtat gcccaccgaa
     8581 caaatctggc agcagagcaa agtcatcgcc ctgttcggcg aacacgacga agccgccgcc
     8641 gtcatcgatg ccgccgcaca agaatgcggg ttaaaagact tggcatacac cggcgaaacc
     8701 atcgaagacc aagactgggt gcgcctcacg caatcgcaat tcgaccccat ccgaatttcc
     8761 gaccgcctgt ggattacccc ctcttggcac gaagcccccg aaggctgcgc cgtcaacctc
     8821 cgcctcgatc ccggactcgc cttcggcacc ggcagccacc cgaccacgcg cctctgcctc
     8881 aaatggctgg atacgcaact caaaaacggc gaaagcgtcc tcgactacgg ctgcggttcg
     8941 ggcatcctga ccatcgccgc cctcaaactc ggtgcaggtt ccgccgtcgg cgtggatatt
     9001 gacgaacagg ccgtccgcgc cggcagggac aacgccgaac agaacaacgt cgatgcacaa
     9061 ttcttcctgc ccgacagtct gcctcaaggg caattcgatg tcgtcgtcgc caacatcctc
     9121 gccaaccctt tgcgtatgct cggcgaaatg cttgcagccc gcaccaaaca gggcggacgc
     9181 atcgtgttgt ccggtttgtt ggatgaacag gccgaagaac tcggcggcat ttacagccaa
     9241 tggttcgatc tcgacccggc ggaaactgac gaaggctggg cgcgcttgag cggcgtaaaa
     9301 cgctgaaacg gaaaggaaac accgtgcagg acaaaaacaa cctctgctgg ctcgatatgg
     9361 aaatgacggg gctgaatccc gaaaccgacc gcattatcga agtcgcgatg attattaccg
     9421 actcggattt gaatgtgttg gcgcaatccg aagtttacgc catccaccaa agcgacgatc
     9481 tgctcgacaa tatggacgag tggaacaccg ccacacacgg caggacgggg ctgacgcagc
     9541 gcgtgcgcga atcgtcgcat accgaagccg aagtcgaaca gaaactgctg gactttatgt
     9601 cggaatggat acccggacgc gccacaccga tgtgcggcaa ctccatccac caagaccggc
     9661 gttttatggt caaatatatg ccgaaactgg aaaactactt ccactaccgc aacctcgacg
     9721 tttccacgct gaaagaactc gccaaacgct ggaatccgcc cattgccaaa agcgtcgtca
     9781 aacgcggttc gcacaaggca ttggacgaca ttttggagag catcgaagaa atgcgccact
     9841 accgcgaaca cttcctgatt tccgccccga aagccgaagc gcaataagaa acaaacaatg
     9901 ccgtctgaaa cgct
//
LOCUS       DAWTCI010000074         9861 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWTCI010000074 DAWTCI010000000
VERSION     DAWTCI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.074"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            120..1385
                     /gene="efeB"
                     /locus_tag="WM481_001748"
     CDS             120..1385
                     /gene="efeB"
                     /locus_tag="WM481_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HFA9081012.1"
                     /translation="MSQNQPAQPTKRNLFKTALAVGAIGAIGGYFGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPRQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPSAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRD
                     FPNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTAQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHMRLANPR
                     DPEFLKKHRLFRRAYNYSRGPASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVGKGGFLGQGLPGV"
     gene            complement(1457..1786)
                     /locus_tag="WM481_001749"
     CDS             complement(1457..1786)
                     /locus_tag="WM481_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HFA9081013.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVR
                     DANGAALQNGDTVILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(1861..3231)
                     /gene="glmU"
                     /locus_tag="WM481_001750"
     CDS             complement(1861..3231)
                     /gene="glmU"
                     /locus_tag="WM481_001750"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFA9081014.1"
                     /translation="MPQNTLNTVILAAGKGTRMYSQMPKVLHCIGGKPMVERVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDAVWVEQTEQLGTGHAVKTALPHLASEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPAGLGRIIRDGSGSVTAIVEEKDASAT
                     QKTIREINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVRPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     VVIEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLEGCEVGENNRIGPYARLRPQ
                     AKLADNVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNIEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(3286..3558)
                     /locus_tag="WM481_001751"
     CDS             complement(3286..3558)
                     /locus_tag="WM481_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFA9081015.1"
                     /translation="MRKTFLILTVSAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERRAVLPDQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(3534..4265)
                     /locus_tag="WM481_001752"
     CDS             complement(3534..4265)
                     /locus_tag="WM481_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFA9081016.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFHLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVAGALGLENCFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHTPPFIDASVSDMAQLARYAETLSER
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            4324..5325
                     /locus_tag="WM481_001753"
     CDS             4324..5325
                     /locus_tag="WM481_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9081017.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSVNYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANIGGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSPEV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPAHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5419..6267
                     /locus_tag="WM481_001754"
     CDS             5419..6267
                     /locus_tag="WM481_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFA9081018.1"
                     /translation="MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQN
                     EERQPAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            6294..6776
                     /locus_tag="WM481_001755"
     CDS             6294..6776
                     /locus_tag="WM481_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFA9081019.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEYRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLVENGGDAV
                     "
     gene            complement(6860..8428)
                     /gene="msrAB"
                     /locus_tag="WM481_001756"
     CDS             complement(6860..8428)
                     /gene="msrAB"
                     /locus_tag="WM481_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFA9081020.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQAEKWAQDAKFSSANLITVASPGFLH
                     EKKDGEFQKWYAGLNYPKLPVVTDNGGTIAQNLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRNPNADLGSLKHSFYKPDTQKKDSAIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTENPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYYFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKAAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRTEVRSRAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKGEVK"
     gene            8574..9827
                     /gene="ftsY"
                     /locus_tag="WM481_001757"
     CDS             8574..9827
                     /gene="ftsY"
                     /locus_tag="WM481_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFA9081021.1"
                     /translation="MFSFFRRKKKQETPALEEAQVQETAAKVESEVAQIVGNIKEDVE
                     SLAESVKGRAESAVETVSGAVEQVKETVAEMPSEAGEAAERVESAKEAVAETVGEAVG
                     QVQEAVATTEEHKLGWAARLKQGLAKSRDKMAKSLAGVFGGGQIGEDLYEELETVLIT
                     GDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPETKEPF
                     VIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGGRNNVTVI
                     SQTAGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKAIPGA
                     PHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRPVPVR
                     YIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2036 a         2603 c         2994 g         2228 t
ORIGIN      
        1 gttaatccgc tatatccgcc atatgttgca gggcgggctt tcagaccgcc gctatcggtt
       61 aatggaaaaa cggcgtgcag ggatgcccac cctgctgcac ggatattgaa ggaaacgcca
      121 tgagtcaaaa ccaacccgca caaccgacca aacgcaatct gttcaaaacc gcccttgccg
      181 tcggcgcaat cggcgcaatc ggaggttatt tcggcggcaa aaaacagggc gaaaccgccg
      241 aacgcaccgc cgaaagccaa cactcgcccc aagcctatcc ctgctacggc gaacatcagg
      301 caggtatcgt tacgccgcgg caggcgtttt ccattatgtg cgccttcgac gtaaccgcgc
      361 aaagtgccaa gcagctggaa aacctgttcc gcacactgac cgcccgcatc gagtttctca
      421 cccaaggcgg agaataccaa gacggcgacg acaaactccc gtcagccggc agcggcattt
      481 tgggcaaagc cttcaacccc gacggattga ccgttaccgt gggggtgggc agcagcctgt
      541 ttgacggccg gttcggactc aaagacaaaa aaacggttca tttgcaggaa atgcgcgact
      601 tccccaacga taagctgcaa aaaagctggt gcgacggcga tttgagcctg caaatctgcg
      661 ccttcacccc cgaaacctgc caagccgccc tgcgcgacat catcaaacac accgcccaaa
      721 ccgccgtcat ccgctggagt atcgacgggt ggcagcctaa atccgaaccc ggcgcgatgg
      781 cggcgcgcaa cctgttgggc ttccgagacg gcacgggcaa ccccaaggtt tccgatccca
      841 aaaccgccga cgaggtttta tggacgggcg tggccgccaa cagcctcgac gaaccggagt
      901 gggcgaaaaa cggcagctat caggcagtcc gccttatccg ccgctttgtc gagttttggg
      961 acaggacgcc gcttcaagag caaaccgaca ttttcgggcg gcgaaaatac agcggggcgc
     1021 cgatggacgg caaaaaagaa gccgaccaac cggatttcgc caaagacccc gagggtaata
     1081 ccacgcccaa agacagccat atgcgcctgg cgaatccgcg cgatcccgaa ttcctcaaaa
     1141 aacaccgcct cttccgccgc gcctacaact attctcgcgg acccgcctca agcggacagc
     1201 ttgatgtcgg gctggtgttc gtctgctatc aggcaaatct tgccgacggt ttcatcttcg
     1261 tgcaaaacct cctcaacggc gaaccgctgg aagaatacat cagccccttc ggcggcggct
     1321 atttcttcgt cttgcccggc gtgggaaaag gcggattctt gggacaaggg ctgccgggcg
     1381 tataaacctg cctgacggac aaatgccgtc cgaaccttgc caaacgggtt cggacgggat
     1441 tttactgttt ggacggtcag gcttttttga cgaattcgga ttttaaattc atcgcgctgc
     1501 cgtcgatttt gcagccgatg ttgtgatcgc cttcctgcag gcgtatgcct ttgaccttcg
     1561 taccttgttt gatcgtcatc gagctgcctt ttaccttgag gtctttgatg aggatgacgg
     1621 tgtcgccgtt ttgcagcgcc gcgccgttgg catcgcgcac ttgagccgca aggtcggcgg
     1681 cggattcggt ttcattccat tcgtgggcgc attcggggca gatgtattgt ccgccgtctt
     1741 cataggtgta ttcggaggcg cattgcgggc aagggggtaa tgacatggtt tgccgtcctt
     1801 atcggatgtt tgttttggga tgccgtctga aacctgaaac cggcttcaga cggcacggct
     1861 ttattgtttg tctttttccg gccttaccca gccttcgatg acggtttggc gggcgcgggc
     1921 gagggcgagt ttgttgtctt cgatattgcg ggtaatcgcg ctgcctgcgc ctgtggttac
     1981 tttgttgccc agtgttacag gggcaaccag tacgcagttc gaaccgatgc gcacttcgtc
     2041 gccgatgacg gttttgtgtt tgtgcacgcc gtcgtagttg gcaataatcg tgcccgcgcc
     2101 gaagttggtt ttgctgccga cttcggcatc gcctatgtag gtgaggtggt tggctttggt
     2161 gcctttgccg atggcggcgt ttttgatttc gacgaagttg ccgacgtgta cattgtcagc
     2221 cagtttggct tgcggacgca aacgggcgta cgggccgatt cggttgtttt cgccgacttc
     2281 gcagccttcg aggtgggaga agggggcgat tttgctgttt gcgccgattt tggcgttttt
     2341 gatgacgcag tttgcgccga tttcgacgtt gtcgccgagt tcgacttcac cttcgatgac
     2401 gacgttgacg tcaatcacga catcctgtcc gtgtttcaga cggccccgca aatcgaaacg
     2461 cgccggatcg cgcagggtca cgcctgcttt gagcaattct tgcgcctgtt cggtttggaa
     2521 gatgcgttcg agttcggcga gctggcgttt gttgttcacg ccggcggcga ggtgggaggc
     2581 gcgcacccgg acggggcgga ctttgatgcc gtcggcaacg gctttggcga tgaggtcggt
     2641 taaataatat tcgccttggg cattgttgct ggaaaggctg ttcagccagt tttccagttt
     2701 ggcgttgggc aggacgagga tgcctgtgtt gatttcgcgg atggtttttt gggtggcact
     2761 tgcgtctttt tcttcgacaa tggcggttac gctgccgctg ccgtcgcgga tgatgcgccc
     2821 caagcctgcc gggtcggcgg gcacgtcggt caacagcccg acttcgttgc ctgcggcttc
     2881 gagcagggtt tcgagggttt caacgtcaat taaaggaacg tcgccgtaca acaccagcgt
     2941 gcggccttcg gaggcaaggt ggggcagggc ggttttgacg gcgtggccgg tgccgagttg
     3001 ttcggtttgt tcgacccaga cggcatcgcg tttgacagtg tctaaaactt gctctttgcc
     3061 gtgtccgacg acgacgcaga tgttttgcgg attcagcgcg gcggcggtgt cgataacgcg
     3121 ctcgaccatg ggcttgccgc cgatgcagtg cagcactttg ggcatttggg aatacatgcg
     3181 cgtgcctttg ccggcggcga ggatgacggt gtttaaagtg ttttgcggca tagcgttttc
     3241 ctgtgccatg ccgtctgaag cggtttcaga cggcataggg taggtttatc ggttttgaaa
     3301 ctttggtttt tgccagtgtt ggcgatgctc ttcgtcggcg ttgttgccgg tttggtcggg
     3361 cagcacggcg cggcgttcgg gacggtattg gttgtagttc atatttttcg agtagctgcc
     3421 gtcttggtaa taaacgggcg tgccggcggg atatttttga cggacggcgg ttttgccgtt
     3481 gccgtcttga taagtttccc acgcgcagcc cgacaaaagg gcggcggaga cggtcaggat
     3541 aaggaaggtt ttacgcatag cattctttcg tattttcggg ggggggtgcg ggtattgtaa
     3601 tgattttggc ggcgttctga caaagtttct gcataccgtg ccagttgcgc catatcgctt
     3661 acggaggcat cgataaaggg cggcgtgtgg gattttgcac cgaaccggac ggttttcatg
     3721 cccaatgctt ttgcctgatg gaggttgtcc gcgctgtcgt ccaccataat gcagcattcg
     3781 ggcggtacgt ccaacaggcg gcagacattg agatacgctt gcggattggg tttgtacagc
     3841 agtccgaaat catccgtgcc gaacagtgcg tcgaaacagt tttccaaacc gagtgcgccg
     3901 gcaacggcac ggacgtaaaa cgacgggccg ttggaaaaaa ccgccttgcg cccgttcaga
     3961 cggcataggg tgttttccgt atccgccatg ccgtgcagcc tggtcaggat tgcatcgatc
     4021 ggatggcttt cgcgtaaaaa ttccgcaatg tcgatttcgg gatggtggat ttgcagtccg
     4081 gcgagcgttg cgccgtaacg gtgccaatag tcttgacgca ggtcggacgc ggcggattcg
     4141 gagagtttga ggcggcgtgc catatagcgc gtcatagcgc ggttgatgag gtggaagatg
     4201 cctgcgtcgg catcgtgcag cgtgttgtcg aggtcgaaca gccacacggt cgggttttct
     4261 tgcattttga accgtgaaaa tttgttagaa tgttatttta cagcgaataa aggaagactc
     4321 ggaatgaaac ggaaaatttg gctgctgccg ctgctggcgg tttcggcata cctgcaggcg
     4381 cagacggaag tcaggctggc ggtgcataag tcgttcagcc tgcccaaagg gctgattgcg
     4441 cgcttcgagc gggcaaacga tgcgaaagtg tcgattattc aggcgggcgg cgcgaacgaa
     4501 atgctcaaca aactgatttt gagccgagcc aacccgattg ccgacgcggt gtacggtttg
     4561 gacaacgcca atatcggcaa ggcgcgggaa atgggcattt tggcggcggc gcaacccgaa
     4621 tccgcccccg tcgcggtcgg gctgccttcg gctttggcgg tcgattacgg ctatgtgtcc
     4681 gtcaattacg acaaaaaatg gtttgaaggc aaaaagctgc ccctgccgca aaccctgcag
     4741 gatttgaccc gccccgaata taaaaaccta ttggtcgtgc cgtcccccgc cacatcttcc
     4801 cccggcttgg gcttcctgat ggcgaacatc ggcggtctgg gcgaagaagg cgcgtttaaa
     4861 tggtgggcac agatgcggca gaacggcgtg aaggtcgcca aaggctggag cgaggcgtat
     4921 tacaccgact tttcgcacaa cggcggcgcg tatccgctgg tggtcggtta tgccgccagc
     4981 cctgcggcgg aagtatattt ttccaaaggc aaatacagcg agccgccgac gggcaacctg
     5041 tttttaaaag gcggcgtatt ccgtcaggtc gaaggcgcgg cggtcttgaa gggcgcgaaa
     5101 cagccgaagc tggcggcaaa actggtgcaa tggctgcaaa gtccggaagt gcagcaggcg
     5161 gttccgtctg aaatgtgggt ttaccccgcc gtcaaaaaca cgcgcctgcc cgacgtgttc
     5221 cgcttcgccc aagccccggc gcacactacc gcccccgcgc agcgcgatat tgatgcgaac
     5281 cagcgcggat gggtttcccg ttggatcagg acggttttga aataaaacaa acataccgcc
     5341 ccgcagggct tcagacggca tttttacacc tgtgccgatt acgccgcacg gggcggatgt
     5401 tcgatcgaga ggaaaacaat ggacttcaaa caatttgatt ttttacacct gatcagtgtt
     5461 tccggttggg ggcatctggc tgaaaaggcg tgggcgttcg ggctgaacct tgccgccgcg
     5521 ctgcttattt tcttggtcgg gaaatgggcg gcgaaacgca ttgtcgccgt gatgagggcg
     5581 gcgatgacgc gcgcgcaggt cgatgccacg ctgattagtt ttttgtgtaa tgttgccaat
     5641 atcggcttat tgattttggt gattattgcc gcattgggca gattgggcgt ttccacaaca
     5701 tccgtaaccg ccttaatcgg cggcgcgggt ttggcggtgg cgttgtcctt aaaagaccag
     5761 ctgtccaatt ttgccgccgg cgcgctgatt atcctgttcc gcccgttcaa agtcggcgac
     5821 tttatccgtg tcggcggttt tgaaggatat gtccgggaaa tcaaaatggt gcagacttct
     5881 ttgcggacga ccgacaacga agaagtcgtg ctgcccaaca gcgtggtgat gggcaacagc
     5941 atcgtcaacc gttccagcct gccgctttgc cgcgcccaag tgatagtcgg cgtcgattac
     6001 aactgcgatt tgaaagtggc gaaagaggcg gtgttgaaag ccgccgccga acaccccttg
     6061 agcgttcaaa acgaagagcg gcagcccgcc gcctacatca ccgccttggg cgacaatgcc
     6121 atcgaaatca cattatgggc ttgggcaaac gaagcagacc gctggacgct gcaatgcgac
     6181 ttgaacgaac aagtggtcga aaacctccgc aaagtcaata tcaacatccc gttcccgcaa
     6241 cgcgacatac acatcatcaa ttcttaaacg ccgtctgaaa gggggggtgg gaaatggacg
     6301 cgttgcacac catcgcccga aacctgacga aaaaacgtca aaccgtaagc tgtgccgaat
     6361 cctgcacggg cggaatgctt gccgccgcat tcacaagcgt tgcaggcagt tcgcaatggt
     6421 tcgaccagag ttttgtaaca tacagcaaca aagccaaaga ataccgcttg ggcgtgttgc
     6481 ccgaaaccct gctcgaacac ggcgcggtca gccgccaaac cgtctatgag atggcgcgcg
     6541 gcgcgaaagc cgtggctcag gcggattacg ccgtcggtat ttccggcatc gccggcccgg
     6601 gcggcggcga aagcaaaccc gtcggcacgg tttggttcgg gtttgccttt ccgggcggaa
     6661 gttgcgaagc aatgcgccgt tttgacggca accgcgaatc cgtccgcgcg caggcggtcg
     6721 ccttcgcgtt ggaacggttg gccgggctgg ttgaaaacgg cggcgatgct gtctgaacaa
     6781 atccccgccg gataaaaaat gccgtctgaa acgttgcagg tttcagacgg cattttgccg
     6841 ggttaggcgg cggtgcggct tatttcactt cgcccttcaa cgcgccgtag cctgccgcgt
     6901 ccatttgttc cagcgggatg aatttcaagc tcgcgccgtt gatgcagtag cgcagtccgc
     6961 ctttgtcgcg ggggccgtcg gggaagacgt gtcccaagtg cgaatcggcg gcgcggcttc
     7021 tgacttcggt gcggcgcata ttgaagctga aatcatcgtg ttcggtaacg gattttgcat
     7081 caatcgggcg cgtgaagctc ggccagccgc agccggaatc atatttgtcg gcggagctga
     7141 acaggggttc gccgctgaca acgtccacat aaatgccggg tttgaacaaa tggtcgtatt
     7201 cgtggctgaa ggcgtattcg gtcgcgctgt tttgggtcac ttggtattgc tcttcggtca
     7261 gggtgcgttt gagttcggcg tcactcggtt ttttatacgt tgccgcgtcg aagcctttcc
     7321 cttgcggtgc ggctttggtt ttgcccggca gcggttcgtc ggctttgcgg atgtcgatgt
     7381 ggcagtagcc gttggggttt ttaatcaggt agtcctgatg gtattcctcg gcgtcgtaga
     7441 agtttttcag cggctcgttt tcaacaacga ggggcagttg gtatttttgc tgctcgcgtt
     7501 tgagggcggc ggcgatgacg gctttttcgg cggggtcggt gtagtacacg ccgctgcggt
     7561 attgcgtgcc ggtgtcgtta ccctgtttgt tgaggctggt cggatcaacg acgcggaaat
     7621 aatattgcag gatgtcgtcc aggctgagtt tgtcggcatc gtaggtcact ttgacggtct
     7681 cggcatggcc cgtatggcgg taggacacgt cttcgtagct cgggttttcc gtgttgccgt
     7741 tggcgtagcc ggataccgcg tcaaccacgc cgtcgatgcg ttggaaatag gcttccaagc
     7801 cccagaagca gccgccggcg aggtagatgg tgcgcgtgtt catgattgct gaatcctttt
     7861 tctgagtgtc gggtttgtag aacgaatgtt tcaaactgcc caaatcggca ttcgggttgc
     7921 ggattaacgc caatgcctgc gcttcgttga tgctgccttt gacgatgcgc tgcacgtcgc
     7981 cgtctttacc gattaacgcc caagaaggat aaacgctgat attcaggttt tgggcgatcg
     8041 tgccgccgtt gtcggtaacg acgggcagct tggggtagtt caaaccggca taccattttt
     8101 gaaactcgcc gtcttttttc tcgtgcaaaa agccggggga ggcgacggta atcaggttgg
     8161 cggagctgaa ttttgcatct tgcgcccatt tctcggcctg tcccaattcg gacaaacata
     8221 aaggacacca gctcgcccaa aatttaatca gcgtcggttt gtcttttttc aaataaacac
     8281 tggcggggcg gttgtccgcg gtttttaacg tggataaagt gtgcggcacg gtcgcggccc
     8341 cggcatcgac gattttgggc gaacaagcgc ccagcgcaag caggcagccg aacttggcgc
     8401 aaagggaaaa gaaagtacgg tgtttcattt tgatgtttcc tgtgtggacg gtttgcatga
     8461 ttagacgttt gagatgccga aaccttacgg cccggatttt cagacaacct tgccgcgcaa
     8521 aatacgctac aatacgccct atttcaagtt tctaaaatta aaaggaaaat tcaatgttca
     8581 gcttcttccg tcgcaagaaa aaacaggaaa cgccggctct cgaggaggcc caagttcagg
     8641 aaaccgcagc aaaagtagaa tctgaagttg ctcaaatagt tggaaatatt aaagaagatg
     8701 tcgaatcttt agcagaaagc gtcaaagggc gggccgaatc tgccgttgaa accgtcagcg
     8761 gtgcggttga acaggtaaag gaaaccgttg ccgagatgcc gtctgaagca ggggaagcgg
     8821 cggaacgcgt tgaatccgca aaagaagctg ttgccgaaac cgtcggcgag gctgtcgggc
     8881 aagttcaaga agccgttgcg acaactgaag aacacaagct cggttgggct gcgcgtttga
     8941 aacaaggctt ggccaaatca cgcgacaaaa tggcgaaatc gctggccggc gtgttcggcg
     9001 gcggacaaat cggcgaggat ttgtacgaag agctggaaac cgtgctgatt accggcgata
     9061 tgggcatgga ggccaccgaa tacctgatga aagacgtgcg cgaccgcgtc agcctcaaag
     9121 ggctgaaaga cggcaacgaa ttgcgcggcg cgttgaaaga agccttgtac gacctgatta
     9181 agccgttgga aaaaccgctg gtcttgcccg aaactaaaga gcctttcgtg attatgcttg
     9241 ccggtatcaa cggcgcgggc aaaaccacgt ctatcggcaa actcgccaaa tatttccaag
     9301 cgcagggcaa atccgtattg ctggcggcag gcgatacttt ccgcgccgcc gcgcgcgagc
     9361 agcttcaggc ttggggcggg cgcaacaatg tgaccgtgat ttcacaaact gcgggcgatt
     9421 ccgccgccgt gtgcttcgat gccgtccaag ccgcgaaagc gcgcgggatc gacatcgtgc
     9481 ttgccgacac cgccggccgc ctgcccacgc agcttcattt gatggaagaa atcaaaaaag
     9541 tgaagcgcgt gctgcaaaaa gccattcccg gcgcgccgca cgaaatcatc gtcgtactcg
     9601 atgccaatat cgggcaaaac gccgtcaacc aagtcaaagc ctttgacgac gcattggggc
     9661 tgacggggct tatcgttacc aaactcgacg gcacggcaaa aggcggcatc ctcgccgcgc
     9721 ttgcttccga ccgccccgtc cccgtccgct acatcggcgt gggcgaaggc atagacgacc
     9781 tgcgcccgtt tgacgcgcgc gcgtttgtgg acgcactgct ggattgagcc gaaatgccgt
     9841 ccgaaaacgg cagaccgccg c
//
LOCUS       DAWTCI010000075         9812 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWTCI010000075 DAWTCI010000000
VERSION     DAWTCI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.075"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..848)
                     /gene="mafA"
                     /locus_tag="WM481_001758"
     CDS             complement(<1..848)
                     /gene="mafA"
                     /locus_tag="WM481_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFA9081022.1"
                     /translation="MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIR"
     gene            complement(979..1698)
                     /locus_tag="WM481_001759"
     CDS             complement(979..1698)
                     /locus_tag="WM481_001759"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFA9081023.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEIVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALNKNLKVMDATAFALCRERKLNIVVFGI
                     AKQGSLKRVITGEDEGTLVHC"
     gene            complement(1915..2769)
                     /locus_tag="WM481_001760"
     CDS             complement(1915..2769)
                     /locus_tag="WM481_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HFA9081024.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVESERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENDTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2898..3626)
                     /gene="rpsB"
                     /locus_tag="WM481_001761"
     CDS             complement(2898..3626)
                     /gene="rpsB"
                     /locus_tag="WM481_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFA9081025.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQETAAE"
     gene            complement(3799..4032)
                     /locus_tag="WM481_001762"
     CDS             complement(3799..4032)
                     /locus_tag="WM481_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081026.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWVVLPTVFTVFA
                     VTVRRRNRLMGQQEQAESEQQRAQRQKDSGTKP"
     gene            4110..4691
                     /locus_tag="WM481_001763"
     CDS             4110..4691
                     /locus_tag="WM481_001763"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFA9081027.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAAAVKINRLLKRYIKRGR
                     KIGVYWPMGKELRLGGFVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGR
                     AKLHVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTV
                     GVGFACQLVDRLPREAHDLPLDGFVSEAGILCF"
     gene            4752..5342
                     /locus_tag="WM481_001764"
     CDS             4752..5342
                     /locus_tag="WM481_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9081028.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEADRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRKRINTCCKIMLLDYAFDVLACCCAGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            5726..7192
                     /locus_tag="WM481_001765"
     CDS             5726..7192
                     /locus_tag="WM481_001765"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFA9081029.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(7379..7753)
                     /locus_tag="WM481_001766"
     CDS             complement(7379..7753)
                     /locus_tag="WM481_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFA9081030.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPINLDKSDNMDTFYGKEGGYVLSTGAMDSKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(7991..8290)
                     /locus_tag="WM481_001767"
     CDS             complement(7991..8290)
                     /locus_tag="WM481_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFA9081031.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(8389..9168)
                     /gene="map"
                     /locus_tag="WM481_001768"
     CDS             complement(8389..9168)
                     /gene="map"
                     /locus_tag="WM481_001768"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFA9081032.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGITTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGGVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPATGKP"
     gene            complement(9208..9801)
                     /locus_tag="WM481_001769"
     CDS             complement(9208..9801)
                     /locus_tag="WM481_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081033.1"
                     /translation="MAKKPNKPFRPTPKLLIRAVLLICITAIGALAVGIVSTFNPNGD
                     KTLQTEPQHTDSPRETEFWLPNGAVGQDAAQPEHHHAASSEPAQPDGTEESGSGLPSP
                     AAPKKNRVKPRPSDAARAADSLTGTGTQAENTLKETPVLPTNAPHPEPRKETPEKQAQ
                     PKETPKEKETPKENHTKPDTPKNTPAKPHKEILDNLF"
BASE COUNT         2058 a         2309 c         2809 g         2636 t
ORIGIN      
        1 cggatatcgg agaaatcgac catcaatcct tccgtcggtt tgattccttt gcttacttta
       61 tacggcccca tccacaatgc gtaattttct ttataggcag cttcaaacgc attggttttg
      121 ggtttgatga gcaatttttt attggttctg tctactgcga aatattccag ttttgtttgg
      181 gctttcagtg tttcggcatt gtataggtgc atttcggttc tgttgcgtat cgttccgaat
      241 acgtcgatgt taataaacac atctgtatcg gcattggcag gagaaacaac gtctatgccg
      301 cgcaggaaaa atacggtctg taccaagtgg gaaagaaagg cagtgtcgcg cgggttggtc
      361 gtcaaggttt catttcgata atcccccatc ccgccaatat ttaagcccag actgctccta
      421 cttccgctac cgtctgattg ggtgcgcgag agtgcagggg cattaagtgt agataaagaa
      481 gtggttaaac ccgtcaaacc gcctgatgtt gtttcagcgg tggtttcgta acgcggatag
      541 gtgtaatcgg tacggacggc agggctgttt atgtattcgc cgcgaatcag tgcatcaatg
      601 gagtagcgac cccctgtcaa actgcctgaa ccttggtcgc ccatagttgc aatgtacaat
      661 gcaacttttc gtccgtgtaa tgcctgtaaa tccatgtctt taacggcagc tctggcagaa
      721 gcggccacaa gttcttgttc gaccgcgaag cgtttgcctc cgccatgcga tggaatacct
      781 gtcagtgtcc cgcaggcgga taaaataaaa actgaaaaaa gaataggtat cagcagccgt
      841 gcccgcatag attttctcct ttatgaaaaa caaattgtat caaaattgta aatttaatcc
      901 actataatct ccatataata aatggctgtc tgaagcccat atgcgactat atctgccgac
      961 cctatggtca atcatcaatc agcagtggac cagtgtgcct tcgtcttcgc cggtaatgac
     1021 gcgtttgagc gagccttgtt tggcgatgcc gaagacgacg atattgagct tgcgttcgcg
     1081 gcagagggca aaggcggtcg cgtccatgac tttgaggttt ttattcaagg cttcgtcaaa
     1141 agtaatggtt tcgtagcgtg tggcggacgg gtcttttttc gggtctgcgg tgtacacgcc
     1201 gtcgacgttg gtggctttga gcatcacgtc gcagttcatt tccgcgccgc gcaatgcggc
     1261 ggcagtgtcg gtcgtgaaga acggattacc ggtaccggcg gcgaaaatca cgactttgcc
     1321 ttcttccaaa tattgaatgg ctttaggacg ggcgtaagtt tcagcgattt gctgcataga
     1381 cagtgcggat tgtacgcgcg ctttgatgcc caaggtttca aatgcgtctt tgagtgccaa
     1441 ggcgttcatc acggtcgcca tcatgcccat gtagtcggca gtggcgcggt ccatgctgcc
     1501 tgcttgtgca gatacgccgc ggaaaatgtt accgccgccg acaacgatac cgacttgcac
     1561 acccattttc acgatttcgg caatttcgcc gacagtttga acaatggtat cgtgattgat
     1621 gccgaacgga tcggaaccca tcagggattc gccggagagt ttcagtaata cgcgtttgta
     1681 tttgatttgc tgtgtcatgg gataccttgc tttcttgaat gtcgtcggtc ggagttggga
     1741 cgtctgaatt tcaaatggtc ggattacaat gatatggtgt gtcgttctat tattgttttg
     1801 ttttcagacg gcctttatat tcggtcgtct taaaataggt accgtaaacg gttttctcaa
     1861 aaaaaagcac cctgattcgt ttggaatcca ggtgctttct ttttcataag tgccttacac
     1921 tttggcggct gcggctactt cggctgcgta gtcgacaacg gctttctcga taccgtcgcc
     1981 tactttgtag cgtacgaagc taaccacttc agtgtcgttt tctttagcga attgggcaac
     2041 agtttggtca gggttcatca cgaatgcttg gccgttcaga gtgatttcag ccaagaattt
     2101 gcggatacgg ccttcaacca ttttagcggc gatgtcggca ggtttgccgg aagcgatggc
     2161 ttgctcggtg tagatgtggc gttctttttc aacggtttca gcatctactt cggcttcgct
     2221 tacgcattgc ggtttggcgg caacgatgtg cataccgatt ttgcgtgcta catcttcaga
     2281 gcctttgtac tcgaccaaaa cgccttcggt cgccaatgcg ccgtggatgt aggcaaccag
     2341 ttggttggca gtgtcaatca cttggaagcg gcggacagac atgttctcgc ccaatttagc
     2401 gatgatggct ttgcgttctg attcaaccag ttcgctcagt tcttcaacag aagccggttt
     2461 tttctcggca gcagttttcg caacgaagtt ggcaaattct acgaagccgg cgtctttagc
     2521 aacgaagtcg gtttcgcagt ttacttcgac caatgcgccg acattaccgt tgattgcata
     2581 agccaatacg ccttcggcag cagtacggcc agccagtttg cccgctttcg caccggattt
     2641 aatacgcagg atttcttcgg ctttgtcgaa gttgccttca gcttcaacca aggctttttt
     2701 gcattccatc atacccaggc cggtagcggc gcgcaggtcg gcaaccattt ttgcagtaat
     2761 ttctgccatt ttgaatctcc tagatttttg ggaaatacgg aaaggatttt ccacattcgg
     2821 gaatcgtgtg ccgcgaacgg gcgttcagac ggcatatttg agaaaagggg cataacgccc
     2881 ctcttctgtg taccgaatta ctcggcagcg gtttcttggg cagcggctac ggtttcttgc
     2941 agcgcttggt ttttgccttc caaaactgcg tcggcgatgc ctcggcagta caggcggatg
     3001 gctttggcgg agtcgtcgtt gccggggatg acgtatttca cgccgtctgg gctgttgttg
     3061 gtatcgacta cggcgataac ggggatgccc agtttttcag cttcaaccag agtacctttt
     3121 tggtagccgg tatcgataac gaaaatcgcg tcgggcaggc ctttcatgtt tttgataccg
     3181 cccaaagagc gttccagttt ttcaacgtcg cgttgcattt ccagaatttc ttttttgctg
     3241 aaaccgcttt cggcagcatt ttccaaggct gcggtttttt cttccaggcg tttgatggat
     3301 tgcttaacgg ttttgtagtt ggtcagcata ccgcccaacc agcggtggtc gacgaaaggc
     3361 ataccggcgc gggtcgcttc ttcgcggatg atgtcgcgtg cctggcgttt ggtacctacg
     3421 aacaataccg tacctttgtt ggcaaccaga cgacgtacgg cttcttgcgc ctcttggaac
     3481 atcggcaggg ttttttccaa gtttacgata tggattttgt tgcgcgcgcc gaaaatgtat
     3541 tgagccattt tcgggttcca gaaacgggtt tggtgaccga agtgaacacc ggcttcaatc
     3601 atctgacgca tagtaatttg agacatgtta tttccttgaa agggttaaag agcacacatt
     3661 caatcgcata gaacttgata ggcggcgcct attgtcaagc acccttcggc gaaagtgggc
     3721 ataagtttta aaaatagtgt gtttccgaaa tggaaaactg acggattata gcggataaga
     3781 tgccgtctga aaagggattc agggttttgt gccgctgtct ttttgccgtt gtgcgcgctg
     3841 ctgttcggat tctgcctgtt cctgctgccc catcaggcgg ttgcggcggc ggacggttac
     3901 ggcgaagacc gtgaacacgg tgggcagaac cacccaaaaa accaaataaa tcaaagcgcg
     3961 cgcaatactc ggctgcgcgg cggaatacat aacggcaaca aaaagatagc cgatggcgac
     4021 gatgtgccac attgtgtgcg tcctgtttga atgttaacaa aatcgttata atcgcaacat
     4081 tctaccgcaa tcctgaaagg ccgtctgaaa tgaggaacga ggaaaaacgc gccctgcgcc
     4141 gcgaattgcg cgggcggcgt tcgcaaatgg ggcgggacgt gcgggcggcg gcggcggtaa
     4201 aaatcaaccg cctgctcaaa cgttatatca agcgcggtcg gaaaatcggc gtgtattggc
     4261 cgatgggcaa ggaattgcgt ttgggcggct ttgtccgcgc ggcgcaaaaa cgcggcgcaa
     4321 aactctatct gccttatatc gaaccgcaca cgcggcggat gtggtttacg ccgtatcctg
     4381 aacgcggaat ggaacgggaa cgcaagcgcg gtagggcgaa gctgcatgtc cctcagtttg
     4441 cagggcgcaa aatccgcgtg cacggtttgt cggtattgct cgtcccgctt gtcggcatag
     4501 accgcgaagg ctaccgtttg gggcaggcag gcggctatta cgatgcgacg ctttcggcga
     4561 tgaaataccg tttgcaggcg aaaaccgtgg gcgtgggctt tgcctgccag ttggtggaca
     4621 ggctcccacg cgaggcgcac gacctgccgc tggacggttt tgtatcggaa gcggggatat
     4681 tgtgttttta gccgttcaga cggcatttcg ggcggaaatg ccgtctgaag gcggaatatt
     4741 gggagaaaag gatgtcggaa aggataatac tgccggtttt gtcattgggc ggcgtacggc
     4801 tcgaaccgtt ggatgtgcat catgaaacgg gtttgcgcga ggcggtttgc gatggggagg
     4861 tttggaagct gggggtaacg tccgcgcccc atccggatag ggtggcagac tatatcggga
     4921 cagcattggc aacgcgtttg gcgtttgcgg ttgtcgatga agaggcggac agggtggtcg
     4981 ggacaacggc gtattatcat tttgaaccgc agattccgcg tttggatatc ggattcacat
     5041 ggtatgcggc atcggcacgg cggaaacgga taaacacctg ttgcaaaatc atgctgttgg
     5101 attacgcatt tgatgtttta gcttgctgtt gcgcgggatg gcgcacggat attctgaacc
     5161 ttgcctcgca acgtgccatc gaacggctgg gcgcggaaaa agacggcgtg ctgcgtatgc
     5221 atatgctccg taaagacggc agtgtgcgcg atacggttgt gtacagtatg ctgagggaag
     5281 attggtgcaa aaacagggaa atcctgaccg gcaggttggc agggtatggc gtgcaggtgt
     5341 gaggcagtat tctcaaattt aaaaaactta acgcgatatg ctgacaatct tttaaaaatt
     5401 aatatataaa caattttgat tttttgtcta ttaaaaattt gatatatata gataaataac
     5461 tatttttatt ttttgtttcg cagttccccg gcttggcggg cgacagaaaa actttgcatt
     5521 tcccagtaaa atccactaca ttcacgataa catattacaa gcccctgaag cggtgtcgga
     5581 agccatactt gcaacgcatt ccaaccccga aacagcgtgc tcggacacac accattgccg
     5641 gtttcccctg cttttgcgat tcaggcgttt gtagtacggc aaataaccat aagggcagaa
     5701 gatgcctgtt ttttaggagt aaaccatggc tgaagcgaca gacgttgtct tggtgggcgg
     5761 cggcattatg agcgcgactt tgggcgtttt gctcaaagaa ctcgaaccgt cttgggaaat
     5821 caccctgatt gaacgcttgg aagatgtggc gttggaatcg tcaaacgcgt ggaacaacgc
     5881 cggcacgggg cattccgcgc tgtgtgaatt gaactatgcg ccgttgggtg caaatgggat
     5941 tatcgatccg gcgcgcgccc tcaatattgc cgaacagttt catgtcagcc gccagttttg
     6001 ggcgacgctg gtcgcggaag gcaagttgga agacaattcc ttcatcaatg ccgtgccgca
     6061 tatgtctttg gtgatgaatg aagaccattg ttcttatctt caaaaacgtt atgacgcgtt
     6121 taaaacccaa aaactttttg aaaatatgga attttccacc gatcggaaca aaatttccga
     6181 ttgggctccg ctgatgatgc gcggccggga cgaaaaccaa cccgtcgccg ccaactactc
     6241 cgccgaaggt acggatgtcg atttcggacg gctgacgcgc cagatggtga aatatttgca
     6301 gggcaagggc gtaaaaaccg agttcaaccg ccacgtcgaa gacatcaaac gcgaatccga
     6361 cggcgcgtgg gtgctcaaaa ccgccgatac ccgcaacccc gacgggcagc tcaccctccg
     6421 tacccgcttc ctcttcctcg gcgcgggcgg cggcgcgctg accctgctgc aaaaatccgg
     6481 catccccgaa ggcaaaggct acggcggctt ccccgtgtcc ggcctgttct tccgcaacag
     6541 caaccccgaa accgccgaac aacacaacgc caaagtgtac gggcaggctt ccgtcggcgc
     6601 gccgccgatg tccgtcccgc acctcgacac acgcaacgtg gacggcaaac gccaccttat
     6661 gttcggccct tacgcaggct tccgttccaa cttcctcaag caaggctcgc ttatggattt
     6721 gccgctgtcc atccatatgg acaatctcta tcctatgctg cgcgccggct gggcgaatat
     6781 gccgctgacc aaatacctgc tgggcgaatt gcgtaaaacc aaagaagaac gcttcgcctc
     6841 cctgctggaa tactaccccg aggcaaaccc cgacgactgg gaactcatca ccgcagggca
     6901 acgcgtccaa atcattaaaa aagactccga aaaaggcggc gtgctccagt ttggtacgga
     6961 gattgtcgcc cacgccgacg gctcactcgc cgcattgctg ggcgcgtcgc cgggcgcatc
     7021 gaccgctgtg ccgctgatga tccggctgat gcaccaatgc ttccccgagc gcgccccgtc
     7081 ttgggaaggt cgtctgaaag agctggtacc gggttacggc atcaagttga acgaaaaccc
     7141 tgaaagggcg gatgaaatta tcgcctatac cgcgaaagtg ttggatattt aagccgaatc
     7201 tttcaagaaa agccgtaccc ccatatggtg ttgatgccgt ccctgccggt tatgcccttc
     7261 atccggcagg gcaggttttg atacacgggg caggtaatcg ttttttccct tttgcgaata
     7321 atggaacggt caaaaaacag cgttttcaga aatgaaaacg ctgttttttg ttgcctgatt
     7381 aacgtgggga acagtctttg aagacgattt ggttgtcagg tgcggtaatc ataataggct
     7441 gtttgcggta ggatttgctg tccattgcgc cggtgctcag cacataaccg ccttctttgc
     7501 cgtagaacgt gtccatattg tcggatttat ccaaattgat gggcatttgc acacgtttgc
     7561 cgttgatgac ggcggaggcg tatgtggtca gaccctgttt gttgaagccg taggttactt
     7621 tgactttttt accttgctgg cagacgtagc tgacggtttt tttggcaacg gtggggttgt
     7681 tcgtgccggc agcagcagcc atactgctga gcgcgattgc ggaagacagg attgcggtgg
     7741 tcagaagttt catttttatt cctttggatg gatgtggagt ttgcccgttt cgagctgttt
     7801 gacattctaa tgtcggcgta tcctgaaata aacaggaaat tggataaagt ttgtaaaaag
     7861 gcgggttcgg gcggttcggc ggtttgaggc aggggcggca ggcgggatac ggcggcctgt
     7921 ccggtgtttc agacggcatt gtttgcgttt ttcggaaatg aaaaaccttc gggcatccgg
     7981 aggttgcggt ttaactttgt ttttttcttt cggacttggt ctgtttggac aattcggcat
     8041 acaggtcggg atggtgtttt ttcaacaggg cggcgatttc catatctttt ttgttgactt
     8101 tcgccaaatc gatgcattcg atatggacga ggcggagcag ttcgcgtacg ggtttgggca
     8161 ttttgcggcc ggtttcgtag cgcgagcctc cagactgggt aacgccgacc ttgccccaga
     8221 agtctatctg gttcagcccg gtctttttac ggatggcttt aacatcttct atattttcaa
     8281 atattttcat aaaacttcct ttaagtttgt ctggttgcgg ggcaaattcc gatttgcttt
     8341 gttttcgtaa tgtgccgggt caagcctgtt tttatattgc atacagcttc agggtttgcc
     8401 ggtggcgggg ctgacggtga ggatttcgta gccggtttcg gtcaccaaga cttcgtgttc
     8461 ccattgggcg gagagggagc ggtctttggt aaccaccgtc cagccgtcgt tgaggatacg
     8521 caggtggcgt ttgccttggt tgatcatcgg ttcgacggta aaaatcatac ccggttttag
     8581 aacgggaccc tgcccttttt ttccgtagtg caacacttgc ggggcttcgt ggaaaccgcg
     8641 cccgatgccg tgtccgcaga attcttgtac aaccgaataa cctgcatttt cggcaacctg
     8701 ctggcaggcg taacctacgc cgcccagcgt cgcgccaggt ttgacggctt ctatgcccgc
     8761 catcatagag gcgtgggtta cgtcgatcag gcgttgggcg atgggggaga ctttgccgac
     8821 ggtaaacata cggctggagt cgccgtggaa accgtctttt ttgatggtga ggtcgatgtt
     8881 gataatgtca ccttctttga gcggcttgtc gtcgggaatg ccgtggcaga tgacgtggtt
     8941 gacggaggtg cagcaggatt tggggtaggg cgggttgccg tagtggaggg gggcgggata
     9001 gccgccttgg acgttgacgt ggtagtcgta aacaagtttg tcgatttcgt cggtggtgat
     9061 gccgggtttg acgaattgtc cgatgtagtc gagggcttcg gcgacgagtt tgcccagctc
     9121 gcgcattttt tcgatttctt cgggggtttt gatgatgatg ccgttcataa tgcttccttg
     9181 gattgcgggt gtgcctgccg tgccgggtca aaagaggttg tcgagaatct ctttatgggg
     9241 tttggccggc gtgtttttcg gggtgtccgg tttggtatgg ttttctttgg gcgtttcttt
     9301 ttctttgggt gtttctttgg gctgcgcctg tttttcgggt gtttcttttc ggggttcggg
     9361 atggggggcg tttgtgggca gtacgggggt ttctttgagt gtgttttcag cttgtgttcc
     9421 ggtgccggtt aacgaatcgg ctgcccgggc cgcatccgaa gggcgcggtt tgacccggtt
     9481 tttcttgggt gcggcagggg acggcagtcc gctgccgctt tcttctgtgc cgtccggctg
     9541 tgcgggttcg gatgaggcgg cgtggtggtg ttcgggttgg gcggcatctt gtccgacggc
     9601 gccgtttggc agccagaatt cggtttcgcg ggggctgtcg gtgtgttgcg gttcggtttg
     9661 gagggttttg tcgccgttcg ggttgaatgt gctgacgatg cctactgcca atgcgccgat
     9721 ggcggtgata cagatgagca atacggcgcg tatcaggagt ttgggggtcg gcctgaaggg
     9781 tttgttcggt ttttttgcca ttttgattgt gc
//
LOCUS       DAWTCI010000076         9623 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWTCI010000076 DAWTCI010000000
VERSION     DAWTCI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.076"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(70..945)
                     /locus_tag="WM481_001770"
     CDS             complement(70..945)
                     /locus_tag="WM481_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9081034.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     SVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKSDSGKKSGSAWRQALLLLGVWAGYGI
                     IDILFKQIAKSGTAFAGNLLVAFALAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDSPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLSLCSI
                     ACLFYWTEVKALFGI"
     gene            complement(949..1641)
                     /locus_tag="WM481_001771"
     CDS             complement(949..1641)
                     /locus_tag="WM481_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFA9081035.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNLQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRRHEERGRQEEAFRREQARREQFYGEQMRREQA
                     FRREFERQASRSRHAYEPSGSGSGRNYILAAYILFGLGAIMQFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYLLGALTARFGIGVLVIIATTVWYFYRIIAGFIRF
                     NDGRAVAPEKWI"
     gene            complement(1663..2142)
                     /gene="ybaK"
                     /locus_tag="WM481_001772"
     CDS             complement(1663..2142)
                     /gene="ybaK"
                     /locus_tag="WM481_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFA9081036.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGPIVLMHGDKQISTRNLARHLGAKHIEPAAPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPDDLNILNPKTIQAAV"
     gene            2217..2579
                     /locus_tag="WM481_001773"
     CDS             2217..2579
                     /locus_tag="WM481_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFA9081037.1"
                     /translation="MDIRYFGTTPRYSEAVCAGGLIFLSGMVPENGETAAEQTADVLA
                     QTDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            2613..3476
                     /locus_tag="WM481_001774"
     CDS             2613..3476
                     /locus_tag="WM481_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFA9081038.1"
                     /translation="MKTLLPFFGVARVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPSLAFTSECVSDGICSVILHHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVQDSENFTALAVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTKNAE"
     gene            3568..4527
                     /gene="ttcA"
                     /locus_tag="WM481_001775"
     CDS             3568..4527
                     /gene="ttcA"
                     /locus_tag="WM481_001775"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFA9081039.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDTINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQNLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(4621..7560)
                     /locus_tag="WM481_001776"
     CDS             complement(4621..7560)
                     /locus_tag="WM481_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFA9081040.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGICTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIQMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTNKDGKPRAVKKADLGRFEDTEHRPAAGYLFGEIF
                     YDSDIEHENVANNQIEGVIVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(7650..9446)
                     /locus_tag="WM481_001777"
     CDS             complement(7650..9446)
                     /locus_tag="WM481_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFA9081041.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYANSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELALLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFVAQLPWRKRTAKSDVPFGISQDYEWIFVFAKSCQFIAATKGKERRYYETDDFPDR
                     PWRTHDLTKQTTAAERPNSFFTMVDPKTGKKYPANPNATWRVTKDTFQDYYNKGKIVF
                     PDDYDFLNISNPVMRYFKDDDMKKAGEDFGKVAVSSRLPENVGTLADAVAEYLAIFSR
                     TLPENIGMTKEGTKEITDLFGSKIFTFPKPSQLIKFLVSISSKSNDLILDFFAGSGTT
                     AHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARKAGYPTIFDITKARIEKAAAKIRV
                     EHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDLPLNDELSEEQLQTLLTTWTLYDG
                     AALTTPVEPVRLGSYTAYLCEKRLYLLNKGFTSADLLAFIKKLDDDADFNPNRVIVFG
                     SNMASAMQHELDQAVRGYTNKKEIELNVIIRV"
BASE COUNT         2254 a         2549 c         2307 g         2513 t
ORIGIN      
        1 ataacaaaat gccgtctgaa gcagcatttt cctgtttcag acggcatttt gttatatcaa
       61 tcagccggat tatatgccga acagtgcctt aacttctgtc caatagaaca ggcaggcaat
      121 ggagcataat gacagtacaa tgcctgccgt attgattgtg ttgatttttt ccttaaagaa
      181 caatgcgccc gaaagcgtcc ctaaaacaat cacaccgata ttcataccgg caaaaaccag
      241 cgtgggactg tccttcatca tttggtgcgc agtgatgtag gttacgatgt tcataaaatt
      301 caaaccgccc aagagtatgc cgccgaccac gctctcaacg cgccatctga ccgatttggc
      361 aaacaggcag gcaaacatca gcacacccgc cagcgcaaat gcaaccagca ggttgcccgc
      421 aaatgccgtg ccgcttttgg caatctgttt gaacaggatg tcgataatgc cgtaacccgc
      481 ccacacaccg agcagcaata acgcctgccg ccacgcgctg cctgattttt tgccgctgtc
      541 gcttttccaa agcaggcaga acagtgcggc aaaggcgagg cacagtccga tcagtttgcc
      601 ttcactgagt ttttcgccaa acagcgtcaa agcggcaaca atcggtaaaa acagcgacaa
      661 acgctgcgcc gcgtcggatt tgacgatacc ggcggattct acggatttgc ccataatgac
      721 aaatacggac ggcagcagca cgcccaaagc ggcaaacagc ggccacgtcg gcaaaaatgc
      781 accgatattg ccgacatccg gcttcaatac cgacagggtc agtatgaccg cgaccacata
      841 attgacggcg accgcctgcg cgatgtcgat ttttttcttc cttgccattt tcagcaaaat
      901 ggaaaccgac acgctgaaca cgatgctgat taacaggtag gccatgctct atatccattt
      961 ctcgggcgca accgccctgc cgtcgttgaa gcggataaag ccggcgatga tgcggtagaa
     1021 ataccagacg gtcgttgcaa taataaccag cacgccgatg ccgaaacggg cagtcagcgc
     1081 gcccaaaaga taaagccaaa atgtgcgcca aaaggtttta atcaggtatt cggtatgtgc
     1141 ggcatagaca atgctgtcca aactgttccg tttcatatag gcgagaatca cgccgactat
     1201 gggcatgaac tgcatgattg cacccaaacc gaacaggatg taggcggcga ggatatagtt
     1261 gcgcccgctt ccgctgccgg acggttcgta agcatggcgc gaacgtgaag cctgccgttc
     1321 aaactcccgt ctgaacgcct gttcgcggcg catctgttcg ccgtaaaact gctccctgcg
     1381 cgcctgttcg cggcggaatg cctcttcctg ccgcccgcgt tcctcatgcc gtctgaagga
     1441 cgcgtcgtat tgcgtccgtt ttgacaggtc ggaaagcgta tcgtaagccc gttggatttc
     1501 tttgaacttt tcttccgcct gcagattgcc cggattgcgg tcgggatggt atttcatcgc
     1561 cagcttgcgg taggcgcgtt tgatttcgtc cgcccccgcc tgcggcgaca cgcccaatac
     1621 ggcatacaaa tctttgtcca tattgctcct tatacttccc ggtcaaaccg ctgcctgtat
     1681 tgttttcggg ttcaaaatat ttaaatcatc ggggcggata ccgataatga acccgcgttt
     1741 tccgccattg atatagatgg tttccaaatc catcaccgac tgttcgacgt aaatatccaa
     1801 ttttgtccgg atgccgaacg gcgttgtgcc gccgaccaga tagcccgtcc acttgtttgc
     1861 ctgcgcaggc gcggcgggtt cgatgtgttt cgcacccaaa tgccgcgcca gattgcgggt
     1921 tgaaatctgt ttgtcgccgt gcatcaagac aatcggcccc ttaccgtttt catcttgcaa
     1981 aacaatggtt ttaatgacca agtgttcgtc tttgccgaac agtcgggcaa actgcgccgt
     2041 gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt tttcacgcaa
     2101 aacgcgtacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc atgcaaaaca
     2161 tggaaatcgg tcaagcgcaa tgttaccata tatccccttt atcgaaggaa accgatatgg
     2221 atatccggta tttcggcaca acgccccgct attccgaagc ggtttgcgcg ggcggcttga
     2281 ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag actgccgacg
     2341 tgcttgccca aaccgaccgc tggctggcgg aatgcggctc ggacaaggca cacgttttgg
     2401 atgcggtcat ctacctgcgt gatatgggcg actatgcgga aatgaacggc gtgtgggacg
     2461 cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagcgcgg ctcgcccgtc
     2521 cggagtggcg cgtcgaaatc aaaataaccg ccgtgaagag agacgcggca actgcataaa
     2581 cagatttcct tatatttcag agtacgcaaa tgatgaaaac cttactgccc tttttcggcg
     2641 tggcgcgcgt cagcggcgaa gaccgccaaa ccttcctgca cggacagctt tccaacgaca
     2701 tcaataatct tcagaccggg caggcgtgtt acgcgactta caacacgccc aaaggccgcg
     2761 tcatcgccaa tatgattgtc gtcaaccgtg gagacgattt gctgctgatt atggcgcaag
     2821 atttgcttga agcaacggtc aaacgcctgc ggatgtttgt gttgcgcgcc aaagccgttt
     2881 ttgaaatcct tgaagattac gccgtcggcg cggaattggc agcatccgcc gaaccgcttg
     2941 ccgcgcaaga acccagtctc gcctttacct ccgaatgcgt ctcagacggc atctgttccg
     3001 tcatcctgca ccatcggggc attttgcaca tcgcccccga aaccgcccta cccccttatg
     3061 acgctgccgc cgaaaacgcg tggcggctgc acgaaatccg cagcggttat ccgtggatat
     3121 gcgcggctac caaagaaacc gccgtcgccc aaatgctcaa ccagcacatc atcggcggcg
     3181 tacacttcaa aaaaggctgc tacccgggac aggaaatcat cgcgcgcgcc cagtatcggg
     3241 ggcaggtcaa acgcggcttg gcggtattgt cgggaaattc ggcagccgaa gcgggcatcc
     3301 tgctgacggc ggacggcgaa gaagccggca tcgtactcga cagcgttcaa gattcggaaa
     3361 actttaccgc ccttgccgtg attaaattct ccgccgcgca aaaagaactc accgtcccaa
     3421 acggcggtat cttcaaagcg gttcatctct tctttaaaac caaaaatgca gaataagccc
     3481 tctaatgccg tctgaagccc ttcagacggc attgccgccc ctgttttcgg ttacaatgcc
     3541 gcatccctaa tttttcaaac caccgccatg tccaaaaaaa ccaagcaaga actcgaaaac
     3601 aacaaactca gcaaacgcct gcgccacgcc gtcggcgaca cgattaacga tttcaacatg
     3661 atcgagccgg gcgacaaaat catggtctgc ctctccggcg gcaaagacag ctacgccctg
     3721 ttagacatcc tgcgccggct ccaagccagc gcgccgattg attttgaact ggtcgccgtc
     3781 aatctcgacc aaaaacagcc gggcttcccc gaagaagtat tgccgaccta tctcgaaagc
     3841 atcggcgttc cctacaaaat cgttgaagaa gacacttact ccaccgtcaa acgcgtgttg
     3901 gacgaaggca aaacgacttg ttcgctgtgc agccgcctgc ggcgcggcat cctctaccgc
     3961 accgcaaaag aattgggctg caccaaaatc gccttgggac accaccgcga cgacatcctc
     4021 gccaccatgt ttttaaatat gttttacggc ggcaaactca aagccatgcc gcccaagctg
     4081 gtaagcgaca acggcgaaca catcgtcatc cgcccgctgg cgtatgtgaa agaaaaagac
     4141 ttaatcaaat acgccgaatt gaaacagttc cccatcatcc cgtgcaacct ctgcggctcg
     4201 cagcccaacc tacaacgcca agtcatcggc gatatgctgc gcgattggga caaacgcttc
     4261 cccggccgta tcgaatcgat gttctccgcc ctgcaaaacg tcgtcccttc ccaccttgcc
     4321 gataccgaac tctttgactt tgccggattg gaacgcggtc aaaatctgaa acacggcggc
     4381 gatttggctt ttgacagcga aaaaatgccc gaacgctttt cagacggcag cgaagaagat
     4441 gagagcgaaa tcaaaatcga gccgcaaaaa gccgaacgca aagtcatcaa cattctggcg
     4501 aacaagccga aaacctgcgg cgcgtaaacc tccaccgtca aaaatgccgt ctgaagcccg
     4561 tttggcgttt cagacggcat tttaaaatcc cgaagcagac ggcttccaaa catttgtttc
     4621 tcagtgtccg ttttcagaat gcccgcctgc tggcatattt tgcccgatca gttcggctat
     4681 cctctcgccg tcaaactgcg ttttgaacac caccttgatt tctttggaaa tctcggcaaa
     4741 cagcttttcg gcatgtttga ttttccgctc ttcgcttttt cgcaaatctt ccgccccgtc
     4801 agtccctttg gcttcaatca caaagttcag aatctcgccg cttttggttt tcacgatata
     4861 ggcaaagtcg ggcgaatacg tgccgccgcc ggcaacaggg attttgatgg agtttctcgg
     4921 tattttggta aatacgatta cgccttcaat ttggttgttg gcgacatttt catgttctat
     4981 atccgaatcg tagaaaatct cgccgaagag atagccggcg gcaggccggt gctccgtatc
     5041 ttcaaatctg cccaaatctg cttttttcac cgcgcgcggt ttgccgtctt tattggtaaa
     5101 tttggtcgga tggattttgc tgccgacaag ccggtaatcc agttcgaatt tatggaagga
     5161 atgatgaagc aaaaaccggt tgaagccgtt tttgatttgg gcgatggtct gcatattcaa
     5221 aaaatcgcca atgttcagtt cgtcgcggac gcggtaaaac gcctgatgca aagtctgcat
     5281 ctggattttt gccgtttgtg ccagtttttc cagaaactca cggtaagtca ttgtgttgaa
     5341 acggataaaa tcttcatctt caatactgtc tatgcggcgg gaaagcataa gcccgttgtt
     5401 gatataagct tcgtttaccg ccgtgcatat gcctgcctgc gggaatttgg cggcgttttc
     5461 acgcaaatag gcggtaaata aatcgacaaa ttcggcttca tctttgattt tgtactgcaa
     5521 aacggcttta tggtgaatca gctcccacaa ggctttgagt tcttcatatt tgccttcgcg
     5581 catgatgatg gtgtctttgc cctcgtcttt ggcgttgctg actttgcctt tgtccaaacc
     5641 tttggggaag gcttcgggat aggcggcttt taatttgtca tagccgtctt cggcaaagtt
     5701 ttcattgtcg tcaatgatgc cgtctgaaaa cagttggttt accaatacca gcggtttgat
     5761 atcggggtat ttttgcaata ttttttgttt cagctcttcg gtaaactttt tggagatttc
     5821 ttcctgaaaa gaattgtcgt tgatttcgcc gacaagctgc ttcacaaagt ctttttcgct
     5881 gctatcgaca aaataattca gtttgtacgg tacatcgcgc acccgcgcca tcagctcgtt
     5941 taccggcagg cgcaggccgc gtccgacttc ttgcagcttg gaagtcgtgc tgccgctgga
     6001 acgcagtttg caaatctgga aaacgttggg attgtcccag ccttcgcgca gcgtccattt
     6061 ggaaaaaata aagcggcgcg ggttgtccaa agacagcagt ttttccttat cgtgcaggat
     6121 ttcattgatt tcctgctcga ttctatcgtc gctgtctgta ttgtctttgg aaaaatagcc
     6181 accgtggcag gcggatacat cgtccaacgt cttttgcagg taatcgcggt aaaacgggtc
     6241 gctttccgtt ttcagacggc gtgccgcttc cgcgcgaatc cagctttcaa atttatcttt
     6301 caggctgcct gaaagctcgt tgccgctgcg gtagcccgcg atatcgtcaa taaaaaacag
     6361 cgtcagcggc ttgattttgg gctgtggcgc gcgttctgcc aaaagcgcgc gttccagctt
     6421 gaaatgttcg gcaaccgccc gctgcatcat cgcatcctgc accgtttgcg aataggaata
     6481 agggttgatg acggcacccg ttttcaactc caagccgttg cttaacacca ccacggtttt
     6541 attcattttg tcgattttca aatccgaaat agccggatgg atttgcgcca aatcttcgcc
     6601 ttttgccagt ttgaacgtct gctttttgtc cttttcgttt aattcaaatt tcgcttcttt
     6661 gccgtccgac gacaccagtt ttaccgccgc atccatgccg ccctgcattt cttcctgaaa
     6721 cacgcgcacg cctttgacca gcccgtcgtt aaacgcgtct actgccgtca aacggtaaag
     6781 caagttgtaa tattcatcgt taaatgttgc accgtagcgc aaaatatatt gcggttttaa
     6841 gcgtttgata ttgccccacg ttttcgcgct atctcgggtc gggaatttat gcggttcgtc
     6901 cacaatcata aacgggcgca cggcagccaa tgcatcaacg ggattgtcaa acaaatcctt
     6961 caatgccttg tcgcccgtat cgttcatgga cgacgaatta accatgcccg cgttaatcag
     7021 cagcacatga atttcctttt tgttttccgc tttgacaaat tgctcaatcg ttatgggcgc
     7081 attggacttt ttgcccttat tctttttcgc gctttccacc acataggttt tcaggcgtac
     7141 gccttcataa tcgccgccga aatcctgttc aaaatgctct gccaaagcct tgctttgcaa
     7201 aaactgctgt gttcccgcct taatggacaa agtcggcacg accacgataa atttgaacac
     7261 gcccagccaa cggtgcagct cgaacatggt ttgtgtgtag gtataggttt tgcccgtgcc
     7321 cgtttccatg gaaatatcaa ggatattttg gtcgtccgaa cggtcgggga atcggccgtc
     7381 tataccgttt tggctttgca ctttcaggat attgtttgcg tattgttttg cagcaaacaa
     7441 aagttcggga ttttcgtctg ccgtccgata tttgggcgtt gccccgtcaa acacgcccaa
     7501 aaccgccgaa accgcccgca tttggtgcgg ctggtttttc tcgtaattaa aaccgctcat
     7561 gaatgtttct ccatgaagtc attgttgtat cgtccttccc ccgcaagggg aatccaagcc
     7621 gaaaagcagc ctgcactttc ggattccggt tacaccctga taatcacatt caactcaatc
     7681 tcttttttat tggtataacc gcgaaccgcc tggtcgagtt cgtgctgcat ggcgcttgcc
     7741 atattgctgc cgaatacaat cacacggttg ggattgaaat ccgcatcgtc gtccagcttc
     7801 ttgataaacg ccagcaaatc ggcggaagta aaacccttgt taagcagata cagccgtttt
     7861 tcgcacagat acgccgtata actgcccaac cgcacaggct caaccggcgt ggtcagtgcc
     7921 gccccgtcat acagcgtcca ggtggtcaga agcgtttgca gctgttcttc gcttaattca
     7981 tcgttaagcg gcaaatccgg ttgttcgggc gaaaaatcct tgtctggatg ctgcctgaaa
     8041 ttgtctgccg tttgaaagat tttgaagccc gaatcgcccg tgtaatcggg atgttcgacg
     8101 cggattttgg cggcggcttt ttctatgcgg gctttggtga tgtcgaagat ggtcgggtag
     8161 cctgctttac gggcttcgga tttttcagcg gttttttcgg gaagctgtac acagatatag
     8221 cggcggttac cgttttgtcc ttcggcgtta agctgcatca cggcgtgggc ggttgtgccg
     8281 ctgcctgcga agaagtctag gattaggtca ttactctttg aacttattga aactaaaaat
     8341 ttaatcaatt gactaggctt ggggaaggta aatattttgc taccaaataa atctgtgatt
     8401 tcttttgtgc cttctttagt cattccgata ttttcaggta gcgtcctact aaaaatagcc
     8461 aaatattcgg ctactgcatc ggctaatgta ccaacatttt caggtaatct actgcttaca
     8521 gctactttgc caaaatcctc gccagctttt ttcatgtcgt catctttaaa gtaacgcata
     8581 actggattgc tgatatttaa gaaatcataa tcatcaggga aaacgatttt tcctttatta
     8641 taataatctt gaaatgtatc tttggttaca cgccaagttg catttggatt tgctggatat
     8701 ttttttcctg tcttgggatc aaccattgtg aaaaaactat ttggcctttc cgccgcagtt
     8761 gtttgtttcg ttaagtcgtg ggtacgccaa ggacgatcgg ggaaatcatc agtctcataa
     8821 tagcgtcgtt ccttgccttt agttgctgca ataaattggc aagattttgc gaatacaaat
     8881 atccattcat aatcctgcga aataccaaaa ggcacatctg atttagctgt tctttttcgc
     8941 caaggcaatt gtgcaacaaa attcccttcc ccaaacactt catcacacaa caatttcaac
     9001 tgcgccgctt cgttatcgtc aatcgagata aaaatcacgc cgtcgtcctt taacagttcg
     9061 cgggcgatat acaggcgcgg atacataaag gtcagccacg cgctgtgcga gtttgagcct
     9121 ttgtcggtga aatctaaaat ccgcgcggct tcgtcttcgt caatattcgc caggagggca
     9181 agttcggcgg gtgtgaattt gcggtcgtcc tgatagacaa agccgtccga tccggtgttg
     9241 tagggcgggt cgatgtaaat catcttcacg ctgtttgcgt aggcgttttt taagtgtttc
     9301 aacacttcca gattatcgcc gcgaatcagc agattttggc tgcctgcatt ttcgggcttg
     9361 gcgttgtgcg ttttgtcttc acttatcagg gtttcgggcg gcaggttgcg aagcaggcgg
     9421 gcatatgatt tgcccagcca gttcatttcg taaaattcgc gtccgatgtc ggtctgtggc
     9481 gcaatttcgg cttgtaatct gtcgataagg aaatttccgt ctgcgtcaaa acaggcggga
     9541 aacagttttt tgagctgttc gagttgggta gagttggcgg taatgccgtc tgaagtgtag
     9601 attgcctcag tgttcgcccc ggc
//
LOCUS       DAWTCI010000077         9291 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWTCI010000077 DAWTCI010000000
VERSION     DAWTCI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.077"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            39..1695
                     /locus_tag="WM481_001778"
                     /pseudo
     CDS             39..1695
                     /locus_tag="WM481_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="WM481_001779"
     CDS             complement(2178..3590)
                     /gene="trkA"
                     /locus_tag="WM481_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFA9081042.1"
                     /translation="MKILILGNGQVGSTVAQNLAAITNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(3688..5210)
                     /locus_tag="WM481_001780"
                     /pseudo
     CDS             complement(3688..5210)
                     /locus_tag="WM481_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            complement(5517..6323)
                     /locus_tag="WM481_001781"
     CDS             complement(5517..6323)
                     /locus_tag="WM481_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFA9081043.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKVSSSEDLKKMNKVGVVTGHTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPAKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEGGQAAK"
     gene            complement(6375..6626)
                     /locus_tag="WM481_001782"
     CDS             complement(6375..6626)
                     /locus_tag="WM481_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081044.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            6728..7498
                     /locus_tag="WM481_001783"
     CDS             6728..7498
                     /locus_tag="WM481_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081045.1"
                     /translation="MDTNNQDKEPNWEKRLENLRQTRRMNTAPAYVPLTKPSEVGKEE
                     PIPKLDDANRDRVLKAYLKKWQEEHNSAVADCGEEVETDPMIVLQENWLNAQASLQMP
                     VSEKHIESRRRIRFDAKKDSAEFETAQIEGGENADAAESGTAADGVAENGEGGAEEAL
                     MPVQINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGMIRTAVQKQMALFT
                     YQLQQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            7644..8813
                     /gene="metZ"
                     /locus_tag="WM481_001784"
     CDS             7644..8813
                     /gene="metZ"
                     /locus_tag="WM481_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFA9081046.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTIAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGGH
                     VVSSRSLFGTTVGFINNIVTKFGIGVSRVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALRIAQWLQE
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITNPWTTTHGRMQPEEKLAAGIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            8908..9114
                     /locus_tag="WM481_001785"
     CDS             8908..9114
                     /locus_tag="WM481_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFA9081047.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAVLF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT         2128 a         2280 c         2713 g         2170 t
ORIGIN      
        1 aatgttaagc agaaacgatt gtctcaaaaa aagcaacttt ggataaatac aatcaagaat
       61 tggctgaact aggtgaaaat gaaaagaaaa aagaagaact agaaaaaaag taaaacagca
      121 agaaaacaaa attcagatca gaaaaaggca catagaacgt ctaaaagaag acatagaaaa
      181 ggttgcggta ctcgaagaag aaatcaaaag atctgaaaag ttcttcgatg aaaacggttg
      241 gcttaaagac agcattatcg acgacaaaac aaaaaacgcc gtcaacctca aaatgagcaa
      301 cggcgcgcgt tggacggtta ccaatgattc aatgctgaaa gagttggatt tgtcggaaga
      361 cgcgcaggtc gaatttagcg acaacaataa attcgtcaaa gtgtccgtga gcaagctcaa
      421 gggcgatggc ggcgtgttca aaatgtatgg cgacatcgtc aaaggagaat cggacaagct
      481 gattacccga aaaggcagcg aagggacgca catcatcgaa tatatggacg atgccaaggc
      541 aaaaacgacg ggaagggaat atctgaaact ggtcgaaaac aagggcaatc aagaagacaa
      601 taaagcatca aataaagcat catacaaatt gaatgtccgc tgtacggaac agggagggtg
      661 gtgttttgcc ttgggtgagt cgggtgcttc caaaaaggtc aatatttcca cagacggtaa
      721 gcgggacttc tacctctacc cggataccct gacgccgggc gcatcgagca gcgtcctttt
      781 cggcgaggcg ttgtatcagt tgaacgcggt ttccgatgaa acgctggtgc agcgtatggg
      841 cgaaatccac gccgacggaa cgccgcagga agacaacaac gtttggatca aacgcgtcgg
      901 cggaaaattc tccggcagcc gcagcgatta ccgcgtgggc ggttacggca accgctattg
      961 gggctttgcc ggcggcttca acaggacggg gttcggcgat aaatggattc attacaaagg
     1021 gctgatgctc cgccacctcc aatcgtccta cgcttccgaa gactacgtcg gcagcggcaa
     1081 aatcttacgg cagggcggca ggtgtttatt ccgcctggct caaccgggaa agcagggctt
     1141 attatgattt ggtcgccaac atcgcccgat ataagggcag ctacgggctg accaattacg
     1201 ccggcaaacg ggtcgaatcg gacgaggcgc gcctgaatgc ctatatgctt tccgccgaag
     1261 ccggtaggcg gatggaaaaa caagacggcg gcaaaaacct atcggtggca gccggaagtg
     1321 cagttgtctt actggtttac gcgcggctac ggctttccgc tgtcaaacgg gctgtctgcc
     1381 gagacggaca acttccgcag cctgatgggg cggttcggct ttcgggcggg cgtggacggt
     1441 ttggacggcg gcaggctgaa catctacggc aagctgatgt acaaacgcga gtttatcggc
     1501 acgatccgcc acaggttcaa cggctctgcc gtggaggaat tcaaacaccg gggcggctgg
     1561 ttggaatacg gcttgggcgt ggtgcgccgc aatgccggaa acggacggca gctttatttt
     1621 gaggcgcaaa ggtcttcgat gcacacgatg cgccagaatt ggcaggtcaa tatgggcgtg
     1681 cgcagtatgt tctgatgcgc ggatacgcct gaaaacaatc cggaacggtc agacgcgttc
     1741 cggattgttt ttttgtcggg gcggtttgct gccatatcaa aacaggcggg cgatgccgtc
     1801 tgaaacttcg tcattcccgc gaaagcggga atctagaacc tcaaactttc agataatctt
     1861 tgaatattgc tgttgctcaa tggtttggat tcccgcaacc tttcgtcatt cccgtgaaaa
     1921 cgggaatcta gaatctctaa agcttcagct aacctttgaa tattgccgct ttccaatggt
     1981 ctagattccc gcctgcacgg gaatgacgat ttggaaatta cccgaaaccc aaaaacaact
     2041 gaaaccgaac aggccggatt cccgctacgc gggaatgacg gggggcggat gccgtctgaa
     2101 gcggtgggct gaagcccact ctactattat ttttgcttcc ataccgcttg gcggcaatgt
     2161 gtccggaatg gagcggttta tccgaaaaag cccattttga cttggatgag tttctccagt
     2221 tcgttcagga tgcgccggcg cgagacgaaa aagatgatgt ggtcaccgtc ttggatgacg
     2281 gtttcggtat ggtgtcccat aatggtttcg ccggttccgg cgcggacgac ggcggcaatg
     2341 tggcagcctt cgggccattt gatgccgctg atgcgcctgc cgatgatggc ggaagttttt
     2401 ttgtcgccgt gcgcgacgac ttcgatggct tccgccgtgc cgcgccggat ggggtggacg
     2461 gcaacgatgt cgccgcgccg gatgtgggcg agtatcgagc cgatggtgat gaggtggggg
     2521 gagacgacga tgtcgatttt gttgccttcg agcaaatcga cgtagcttga gcggttgacg
     2581 atgccgatga cgcgcttcgc gccgaggttt ttcgccaaaa gggcggacat aatgttgctt
     2641 tcgtcgtcgt tggtcagggc gcagaatacg tcgatttcgt cgatgtattc gttgtcgagc
     2701 agggtttcgt cggttgccga accttgcagg acgagggtgt tgtcgaggtt ttcggctatc
     2761 cattcggcac ggcgcggccg gcattcgatg attttgacgt tgtatgcgtg ttcgagctgc
     2821 ttggcgaggc ggtagccgat gttgccgccg ccggcaatca tgatgcggcg ggtgctggtt
     2881 tctttggggc gcaattcggg tatgaccgcc ccgatgtttt cggcggcggc ggcaaacagg
     2941 atttcgtcgc cttcgatgat gacggtttgc ggcgcgggga cgatgaggcg gttgttgcgg
     3001 taaacggcgc agatttggca gtcggccccg tcgggcaaat cttgggcgat gtcggcaatg
     3061 ctgcgtccga caagcagccc gccgcgccgc gcctgtatga tgaccatccg cacgcggtcg
     3121 tctgcaaaac gtaaaacctg caatgcgccc gggcagtcta tcaggccggc aagctgttcg
     3181 gtaacgagct gttcggggct gatggtttcg gttatgccga atatggaaag gctgccgttt
     3241 tcgttgtttt cgagcttggg gctgaggtat tcgaggtatt cgctggaacg gacgcgcgcg
     3301 atgcggccgg ggatgttgaa caggtcggcg gcaactttgc aggcgacgat gttggtttcg
     3361 tcgctgcggg agagcgcgag cagcaagtcg gcatcttccg cgccggcgcg ttctaatgtg
     3421 aagggggatg cgccgttgcc gaaaacggtt tggacatcga ggcggctgcc tgtttcctgc
     3481 aatgcttttt cgtcgatgtc gataacggtt acgtcgttgt tggtgatggc ggcaaggttt
     3541 tgcgcgacgg tagaacctac ctgcccgttg cctaaaatga ggattttcac ggtatgggtc
     3601 gccgggtgat gaaatggtgg attgtaccgc aaccggcggc ggaaatgccg tctgaaggca
     3661 gattttgcgt tcagacggca ttgcgcttca agactcgacg gggatgatgc cgattttcgc
     3721 ctgccatttg cgcggggcgg tggcgtggat ggattcgcct ttgctgtcca cggcgacggt
     3781 tacgggcata tctttgactt caaattcgta aacggcttcc atacccaatt cggggaacgc
     3841 caagactttg gaagatttga tggcttttgc cacgaggtat gccgcgccgc cgactgccat
     3901 gaggtacacg gccttgttgt cggcgatggc ttcgcaggtg gccgcgccgc gctcggattt
     3961 gccgatcatg cccaagaggc cggtttgttt gagcatttgg cgggtaaatt tgtccatgcg
     4021 ggtggctgtg gtcggacctg cgggaccgac gacttcatcg ccgaccggat cgacggggcc
     4081 gacgtagtaa atcaggcggt tggtgaaatc gacgggcaac tcctcgcctt tgtcgagcat
     4141 attgacgagg cgtttgtgcg cggcatcgcg gccggtgagg attttgccgt tcaacagcaa
     4201 tacgtcgccg gttttccagc ttgccacttc ttctttggtc agcttatcga catcgacgcg
     4261 tttgccgttg tcggggctgt aagtcagatc gggccagtct tcgacgcgcg gcggcgtgag
     4321 ttcgacagga cctgagccgt ccaattcaaa ttcgacgtgg cgggtggcgg cacagttggg
     4381 aatcatggca atcggtttgg aggcggcatg ggtcgggtaa tcgaggattt tcacgtccaa
     4441 cacggtggtc agaccgccca agccttgcgc gccgatgccc agcgcgttga ccttttcaaa
     4501 gagttcgagg cgcagggctt cggtggtgga caattccgcg ccggacgcgg ctttttcctg
     4561 caattcttgg atgtcgatgt ggctcatcag ggattctttc gccatcaaca cggctttttc
     4621 gggcgtgccg ccgatgccga tgcccaagat gccgggagga caccagcccg cgcccatcgt
     4681 cgggatggtt ttcaataccc aatcgacgat gttgtcggaa gggttgagca taggagtttg
     4741 gatttgtttt cagagccgcc gccttttgcc gcgcaggtta cttcgacttt accgcccggc
     4801 acgatgctca tgtggatgac ggcgggggtg ttgtctttgg tgttttggcg tttgccggcc
     4861 ggatcggcga ggacggaagc gcgcagggtg ttgccttccc aagtgtaggc gcggcgtacg
     4921 ccttcgttaa ccatctcttc cacgctcatg tccgcatccc attgcacatc cataccgact
     4981 ttgaggaaga cggttgcgat acctgtgtct tggcagatgg ggcggttgtt ttcggcacac
     5041 atacggctgt tgaccaaaat ctgcgtcatc gcgtctttgg cggcgggatt ttcttccttc
     5101 tgccacgcct tataaagcgc gtcgatgtag tcttttggat ggtagtagct gatgaattgg
     5161 aaggcatcgc agatactttg aataaagtct tcttgcttga taacggtcat gattgtgttc
     5221 cttttcaagg ttgtggcggt aagtgtctgc tgctgtttta aacaaccttt ttgaaggctg
     5281 ttttttatca ggttgaaata gtgccagcgt ttgtacggtg aagacaatac ataattctaa
     5341 tctcttgatt atcaatttgt tttaataaaa tactgtttta ttcggatttt gatatctgta
     5401 atctttgcct gttgctttgc ggtagggaga aatcagcagg cagttcattg tggaccggtt
     5461 gatgaacaat gccgtctgaa agggcttcag acggcatttt gttcggacgg gcgggcttat
     5521 ttcgcagcct gtccgccctc tttggcaaaa tatttggcgt agatcttgtc gtattcgccg
     5581 ctttcgcgta ctttttccaa cgcatcgttc agcattttga cggttgcttc gtcgcctttg
     5641 cgtaccgcga tgccgtagtg ttcggtggtg aagtcgggca gggtaacgaa gtccattcct
     5701 ttggccgggt tgtttttcac ataattggcg atgaccgcgc tgtcgctgac cacggaatcc
     5761 aagccgccgt tttccagttc tttgataatc agggggacgt tttcgaagcg cgcgattttc
     5821 ggattgtcgt tgcccaagag tttggaaacg gagaaatcgc ccgtgtggcc ggtaaccacg
     5881 ccgactttgt tcatcttttt caaatcttcg gaagaagata cttttttgcc tttcggaacg
     5941 aggacgactt gggtgatttc aaaatacggg tcgctgaaat ccatagactg tttgcggtcg
     6001 tcggtaatgg ttacgcccga catcacaacg tccgcatcgc cgttgttcaa ggcggggaaa
     6061 aggctgtccc acggctggtg tttgaattcg attttaaaat tgcccgcctt cgccatcgcg
     6121 ttcatcaaat ccacgtcgaa accttcgaca ttgcctttcg agtctaaaga ttcaaagggg
     6181 gcaaactcgg cgttggaagc cacgcggtac actttgccgg ggttggcggc aggcgcggcg
     6241 gcatctttgc cctgaccgcc gcaggcagac agcgcgagtg cggaacaggc aagggcggcg
     6301 gcaatccatt ttttcatatt catcaaaatt cctttcggta atcaaaaagc ctaaaaaatc
     6361 agtttaacat aaaattaacg ttccttttca cggaaaaacg cataagtaac gtcgtggaac
     6421 aaatccgtat gcgggctgtc ctcatagctc aattctgcca aggcgtcctc aaaagcaagc
     6481 tgaatcgact cgaccgcgat ttcggcggtg tctgcgggaa gggcgcggat cagggctttg
     6541 aaggcggtgg acaggacgtg gttctgcata cgcagcacat cgtttgcttc ttccagatat
     6601 tccaaacgct gttcgatgct gcccatgatg tatcctcgcg aatctgttaa aatgcgccat
     6661 tatatcaaac tgcgctttgg gcgcgggttt gcccgcctgc aaagattaag aaccggaagc
     6721 cgaaaaaatg gatacaaaca atcaggataa agagccgaat tgggaaaaac gcctggaaaa
     6781 cctgcgtcag acgcgccgta tgaataccgc gcctgcctat gtgccgctga ccaaaccttc
     6841 tgaagtcgga aaggaagagc ccataccgaa attggacgat gcaaaccgag accgtgtgtt
     6901 aaaggcgtat ctgaagaaat ggcaggaaga acacaacagc gccgttgccg attgcggcga
     6961 ggaagtcgaa accgacccga tgatcgtcct tcaggaaaac tggctcaacg cgcaggcttc
     7021 cctgcaaatg ccggtttcgg aaaaacacat cgaatcgcgg cggagaatcc ggttcgatgc
     7081 caaaaaagac agtgccgaat ttgaaaccgc acagattgag ggcggggaaa acgccgatgc
     7141 ggcagaatcc ggtacggcgg cagacggggt tgcggaaaac ggcgaaggcg gtgcggaaga
     7201 ggcgttgatg cccgtccaaa tcaatatcct gaacccgaaa gccgtcaacc ggcgcgaagt
     7261 gttctgcctg tccgaacagg agctgaccga acggttgatc aaacgcctgc gcccgcatct
     7321 gaccgatacc gtcaacggca tgatccgtac ggcggtgcag aagcagatgg cgctctttac
     7381 ttatcagctc cagcaaatgc tgcacgagca ggcgggtgcg gtagtcgaag acgtgttgga
     7441 acacgatgtc cgtaaaatcc tcaacgacat caagtacgaa ttaaaataca aacgctgatt
     7501 gccatgccgt ctgaaagccg tgttcagacg gcattttcct gcccgtccga cttgaaagcc
     7561 tcatggaaca ggcgcaaaat attaataaat taaacaacgg gtgcacgccc cgttgccgaa
     7621 cggacaaaac cgaggaatca ccgatgagca aaaaacttca tccgcaaacg ctcgccatac
     7681 gcggcggcaa agaacaaacc ggataccgcg aacacaatca ggcattgttc ctgaccagca
     7741 gcttcatgtg ggacaacgcc caacacgctg ccgatttgtt ttcaaaaaaa atcaaaggct
     7801 tcacttatac ccgtaccgcc aacccgacca tcgcggcctt tgaaaaacgc atagccgctt
     7861 tggaaggcgc ggaacgcgcg gtcgccactt cgaccggcat gtccgcgatt caggcggcgt
     7921 ttttcacctt cctgcaggcg ggcggccatg tggtttccag ccgcagcctg ttcggcacga
     7981 ccgtcggctt tatcaataac atcgttacca aattcggcat cggagtgagc cgcgtgtcgc
     8041 cgactgatat aaatgaatgg aaagccgccg tcaaagccaa caccaaactg ctgtttttgg
     8101 aaacgccgtc caaccccttg ggcgaagtgg ccgacttgga agccttggcg gaattggcgc
     8161 acggcatcgg cgcgctcttg gtggtggaca acagcctgct gtcgcccgtc ggctcgcagc
     8221 ctttgaaaca cggcgcggat atttccgttt cctccgccac caaagccata gacggacacg
     8281 ggcgcgtgat gggcggcgtg ttggcgggtt cggaagaact gatggcgcag gtcgccgttt
     8341 actgcaactc ttgcggtttg gcgatgtcgc cgtttaacgc gtggcagttg ttgagcggcg
     8401 tggaaaccct gtcgctgcgt atggaaaaac agtttgacaa cgccttgaga atcgcgcaat
     8461 ggctgcaaga gcagccgcag gttcaagcag tgtactacac cggattgccc gaccatccgc
     8521 aggcggagct tatccgcaaa cagcaaaacg gcggcggcat cgtcatcggt tttgaagtcg
     8581 ccgaccaagc agccgcgtgg aaagtcgtgg acggcgtgga actcttttcc cgtaccgcca
     8641 acctcggcga cgtgcgctcg accatcacca atccgtggac aaccacgcac ggcaggatgc
     8701 agcccgaaga aaaactcgcc gccggtatcc gccccggact ggtgcgcctg tcggtcggtt
     8761 tggaatatgt tggcgatttg attgacgatt tgaaacaggc actcgcgcgc tgattgcgtg
     8821 tttcagacgg catcggcgga aaaatgcggc agatgccgtc tgaaaccctt attgcgtatg
     8881 gcatacacat caacaatagg aaaaaatatg gatgaagcgg tttttgccga ttgggcgttg
     8941 aaaatctgcc tgaccggttt gattattttt ttgggtttta tcgtttggaa tttgggcaag
     9001 gagtccaaag caggcaaatt cggcatcgcc gttttgtttt tggttttggg cttgggtgtg
     9061 ttcggttttg tgttcaaaga gttattgatt aagtttttgg tattgcccaa ataggtttgc
     9121 cgtctgtcga aatgccgtct gaaatcataa aaagtaatct gcaccctaaa agtttgattt
     9181 aaccgccaac tgattaaggt gcgggttttt atgacaaaat aaaaatcatc tttccgcaat
     9241 cattcccacg gatgcgaaag cctgtctttt tgaatcccgg ataattttac a
//
LOCUS       DAWTCI010000078         9223 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWTCI010000078 DAWTCI010000000
VERSION     DAWTCI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.078"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="WM481_001786"
     CDS             complement(102..2954)
                     /gene="gcvP"
                     /locus_tag="WM481_001786"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFA9081048.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTLPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIANRIHTLASVFADALVSDGLKVVHEVFFDTVTVDFGSK
                     EKADQVFAAALESGYNLRRVNNTQVAAAFHETSVYEDLADLYRAFTGKDTFTFADDVK
                     GRLNAELLRQDDILQHPVYNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWTEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     SVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRIAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(2985..3344)
                     /locus_tag="WM481_001787"
     CDS             complement(2985..3344)
                     /locus_tag="WM481_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081049.1"
                     /translation="MTDRPNLLRIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNVVFPLPAHPLAWLVPAGFMVLGGGFPLSLPLVALLVPTCCILARCPPLS
                     RLLCHPCPNHPMSKNSA"
     gene            3292..3522
                     /locus_tag="WM481_001788"
     CDS             3292..3522
                     /locus_tag="WM481_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFA9081050.1"
                     /translation="MISACPGFIRSRFGRSVMPSEPAGRLSQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVSQSVHDYINNLGADKPLFI"
     gene            complement(3515..4156)
                     /locus_tag="WM481_001789"
     CDS             complement(3515..4156)
                     /locus_tag="WM481_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFA9081051.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGNNNLKSDQIHANGKGYRKFAENLNQFLRKHGFR"
     gene            complement(4146..4697)
                     /locus_tag="WM481_001790"
     CDS             complement(4146..4697)
                     /locus_tag="WM481_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFA9081052.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIKDDNGLFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLTDAGIVRNRLKIDAAIF
                     NARQIQALQQEHGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRKTLKYHPKWLDAV"
     gene            complement(4701..5219)
                     /locus_tag="WM481_001791"
     CDS             complement(4701..5219)
                     /locus_tag="WM481_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFA9081053.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRINTAQNHVWADTLDDMLEAALSNAFNRLDSTRTFVPAS
                     RSGSTDKWTVYIDAFQGSYTGKTLISGYAVLPDGKNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="WM481_001792"
     CDS             complement(5219..6880)
                     /gene="pqiB"
                     /locus_tag="WM481_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFA9081054.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEIANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGGKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATRGGGLDDLQAKLADLLDKFNNLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGNPQTQNIPNELNQTLKELRITLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNNSSKDPIPKGSR"
     gene            complement(6873..8333)
                     /locus_tag="WM481_001793"
     CDS             complement(6873..8333)
                     /locus_tag="WM481_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HFA9081055.1"
                     /translation="MGLSAGGAAAQKIFLPDTKGLFILPNLPVLHPDWVYRFFSFIMP
                     PFIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRTDVPQLDKGEAAFCPR
                     CGHKLFRVGSHPFSGPPAYAAASLILMAFAYSMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKQAYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAKVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGNN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVVLYFPA
                     NILPIMISSNPAATEANTIFSGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     AARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
     gene            8518..9147
                     /locus_tag="WM481_001794"
     CDS             8518..9147
                     /locus_tag="WM481_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFA9081056.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDPELNKAISELEYMARYDAVTQDLKDLGEEPYKFDKELPHE
                     TGNKAVGWLYCAEGSNLGAAFLFKHAQKLDYTGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAQEAFAFYKVILRETFGLPEGTEAPEGMMPHRH"
BASE COUNT         2049 a         2385 c         2636 g         2153 t
ORIGIN      
        1 agttatagtc gagatacgtg tatcggtgca accggtctgc cgttgacgaa aatgccgtct
       61 gaaagtgttt cagacggcat ttgtcttcgg ggtttcaaca gtcagtcttc ataattttcc
      121 atcggcgggc agctgcacac gaggttgcgg tcgccgtaca cgtcgtccac gcggttgacg
      181 aagggccaga acttgtgttc gcgcacgaag ggcagcggga agacggcttc ttcgcgagag
      241 tacggatgcg cccattcgcc ggttatatct gcggcggtgt gcggcgcgtt gaccagtggg
      301 ttgtcgtctt tcggccattc gccgtcgatg actttctgca cttcgcggcg aatggatttc
      361 agggcggcga tgaagcggtc gagttcggct ttgctttcgc tctcggtcgg ttcgatcatc
      421 agcgtgccgg caacagggaa ggaaaccgtc ggcgcgtgga agccgtagtc catcaggcgt
      481 ttggcgatgt cggtttcggt gatgccgctt tcggctttga gcggacgcaa atcgacgatg
      541 cattcgtgcg cgatgcggcc gtttttgcct gtatacagaa tcggatagtc ttcgctcaag
      601 cgtttagcga catagttggc gttgagcaat gcccagcgtg ttgcctgttc catgccttgt
      661 ttgcccatca tggtcaggta catccaagta atcggcagga tggatgcaga accgaaggct
      721 gcggcggcaa cggaggtttg cccggcactt gcgctgtggg tgtcggtcag ggtatgcccc
      781 ggtgcgaacg gggcgaggtg ggctttcaag ccaatcggac ccatgcccgg gccgccgccg
      841 ccgtgaggga tacagaaggt tttgtgcagg ttcatgtgca acacgtccgc gccgacttcg
      901 gcgggctgca tgatgccgat ttgggcattg aggtttgcgc cgtccatgta aacctgtccg
      961 ccgttttcgt gaatgatccg gcagatgtcg cggatgcctt cttcgtacac gccgtgtgtg
     1021 gacggatagg taatcatgat ggcggacaag gcatcgcggt gttgttcggc tttggctttc
     1081 aaatcgtcaa tattgacgtt gccgtgttcg tcggtatcga cgacgacgac tttcaaaccg
     1141 agcatggcgg cggtggcggg gttggtgccg tgggcggatt tgggaatcag gcagatgttg
     1201 cggtgtgctt cgccttgggc ttcctgataa cggcggatgg cgagcatacc gctgtattcg
     1261 ccttgtgcgc cggagttggg ctggaaggaa atcgcgtcaa agccggtgat ggctttcagg
     1321 ctgttttcca tatcagcaag caattcgcgg tagccggcgg tttgcgcttc gggagcgtaa
     1381 gggtggatgt cggagaactc agtccaagta atcggcaaca tttccgcagt cgcattgagc
     1441 ttcatggtgc agctcccgag cgaaatcatg ctgcggttca tagccaagtc gcggtcttcg
     1501 agttttttca ggtagcgcaa catttcgtgt tcggtgtggt aactgttgta cacaggatgt
     1561 tgcagaatgt cgtcttgacg cagtaattcg gcgttcagac ggcctttgac atcatcggca
     1621 aatgtgaacg tatccttgcc ggtaaacgcg cggtacaaat cggccaaatc ttcgtatacc
     1681 gacgtttcat ggaatgcagc cgcaacttga gtattgttga cgcggcgcag gttgtaaccc
     1741 gactccaaag cagcggcaaa cacttggtct gctttctctt tgctgccgaa atcgacggta
     1801 acggtatcga agaagacttc gtgaaccact ttgaggccgt ctgaaaccag cgcgtcggca
     1861 aagacggaag ccagtgtgtg aatgcggttg gcgatgcgtt tcacgccttt ggggccgtga
     1921 taaacggcgt acatgcccgc caagttcgcc agcaatgcct gtgcggtaca aatattggat
     1981 gtcgcttttt cgcggcggat gtgttgttcg cgggtggaca aagccatgcg caaggcaggt
     2041 ttgcccgatg cgtctttgga tacgccgatg atgcggcccg gggcggagcg tttgaactcg
     2101 tctttaaacg cgaaataagc ggcgtgcgga ccaccaaagc ccatcggtac gccgaaacgt
     2161 tgggtgttgc ccaaagcaat atctgcaccc aattcagccg gagacttcag caaaaccaag
     2221 ctcatgatgt cggcggcaac agcaacgatt gtgcctttgg ctttcagacg gccgataacg
     2281 tcctgcaagt cttgcacgtc gccgtcttta ccgacgtatt ggaacagcgc gccgaagtat
     2341 tcgccttcat ccgcttgggc aaaatcgctg accaccagct cgaagccgaa atatttggcg
     2401 cgggttttca tcacgtccaa agtttgcgga tacacgcgcg catccacaaa gaaacgctcg
     2461 gatttcactt tgcccacgcg gtgcgccatc gccatcgctt cggcggcggc ggtcgcttcg
     2521 tccaacaaag acgcgcccgc cacagggaaa ccggtcaaat cgatgcacac ctgctggaag
     2581 ttcaacaacg cttccaaacg accttgggca atctccgcct gatacggcgt gtaggcggtg
     2641 taccagcccg ggttttccaa tacgttgcgc aaaatcacgt tcggcacgcg ggtcgggtaa
     2701 taacccaagc cgatatagga cttgttgatc acgtttttcg acgcgatgcc tttcaatttc
     2761 gccaatgcgt ccgcctcggt cagggcttcg ggcaaatcga gttcggacgg catacggatg
     2821 ctttgcggca aggtgttgcc gacaaagtcg tccatgctct tctcgccgac agcggcaagc
     2881 aacgccgctt cgtcgccgaa actcaaatgg cgcgcggcaa attcgttggg gttgaacagt
     2941 tcggacagtt tcatttctac tccttcgggt ttgtagggct gccatcaggc agaattttta
     3001 gacatcggat gattcgggca agggtggcac aaaaggcggg ataatggcgg acaacgcgcc
     3061 agaatgcagc aggtcgggac aagcagcgcc acaagcggca gcgataaggg aaagccgccg
     3121 cccaaaacca tgaaacctgc aggcaccagc caagccaagg gatgagccgg aagcggaaaa
     3181 acaacattga gcagaaccaa aatcacgaca aacagcggca gccccgccat caggttggca
     3241 cgcctgtatg ttgcgtacgg gtcggatgct tcttcaggtt cgcaatattc catgatttca
     3301 gcctgtccgg gctttatccg cagcaggttc ggacggtcgg tcatgccgtc tgaaccggca
     3361 ggccgtcttt ctcaaaaaac gccataccgc tgcaacttca tgcggctgta tggcgtaggt
     3421 tttgcacttc agacggcata tgcgtcccga agcagtttgg tgtcccagtc ggttcatgat
     3481 tatataaaca acttgggtgc ggataaacct ttgtttatct aaacccatgt tttctcaaaa
     3541 attgattcaa attttcggcg aatttccgat agcctttgcc gttggcgtgg atttggtcgg
     3601 atttcagatt attattgccc aaaatttccg cccacgcgcc gccgaacaac ggaatgccgt
     3661 attcctcgga caaatcctca tacagcggat ggtcgctcaa atgcccgaac aacgcgccca
     3721 gtgtgatgtg cggcacgccg acgaggacgg cgggaatgtt ttccttttgc acggtttcga
     3781 tgattttcgc gatattggcg cgggtctgct cctcgggaac tttgcgcaga aagtcgttgc
     3841 cgcctatgcc gacaatcaca agcttgggtt tgcgtgccaa cagcgcgggc aggcgcgata
     3901 gggcttgcgc ggacgtatcg cccgatacgc cgccgttgac aatattccaa cccgtcagtt
     3961 tttgcagttg cgcggggtag gattcgccgg ggtttgctcc gtagccgaag gtgagcgaat
     4021 cgcccaaggc aagcacggtg cttccttcgg gaattttggc gtgggttcgg gcggattttc
     4081 tgccgcaggc ggtaagaagc aacgcgcctg cgccgaggag gaaggttctt ctgttcattg
     4141 gtttttcaga cggcatcgag ccatttcggg tggtatttca gggttttacg gtaaaccgga
     4201 cagctttcgg cgtgcgcgcc tttgaggtag ccggtactca tcagaaattc gccgacgatt
     4261 tcgccgccga cgaatttgaa atgtttttta aagagtttga cccattcgtc tttgcttcgc
     4321 ggatggtgcg cgtcgagcca gttcttgaac gaaccgtgtt cttgttgcaa cgcttggatt
     4381 tgccgtgcat tgaaaatggc ggcatcgatt ttcagacggt tgcggacaat acccgcatcg
     4441 gtaagcagcc gttcaatatc ggcttcgcca aatgcggcga ccgtatcgat gtcgaaacct
     4501 tcaaatgccg tctgaaacgc ctgccgcttc ttcagcatca gcgtccaact taatcctgcc
     4561 tgattgattt ccaacaccag ccgttcaaac agtccgttgt cgtccttaat cggaaaaccg
     4621 tattgcgtat cgtggtaatg tttgttcggg ttgtcggtgt tttcgggaag tgaggcggca
     4681 aattcgcaat agttcacggt ttactcgacc atctgttgcg ccgcctgttt cagtccctgt
     4741 tcgagtgcgg cggtcatggc ggcgtagccg tcaccctgct gttcggtttc gatatggaag
     4801 ggtctgttct taccgtcggg taggacggcg tagccgctga tgagggtttt gcccgtgtag
     4861 ctgccttgga atgcgtcgat atagaccgtc catttgtcgg tactgccgct gcgtgaggca
     4921 ggaacaaagg tgcgtgtgct gtccaaacgg ttgaatgcat tgctcaacgc cgcttcgagc
     4981 atatcgtcca aggtgtctgc ccaaacatgg ttttgtgcgg tgttgatgcg gtaggggtcg
     5041 gtttgataga ccagtccgcc gcgtttgagc ggttcggcaa gacggacttc gacggcggtt
     5101 tcgccgcctt gcgttgcagg acggatgtag cggctgtcgg gcaacacgaa atattgtgtg
     5161 ctttgcacag taccgcaggc ggcaagcgtc agggcggcgg cgatggggaa aaggcgcatt
     5221 atcggcttcc tttcgggata gggtctttgc tgctgttgtt gaaaatcagt gcgttgggtt
     5281 tttctttcaa agtgttaatg acgggttgaa cgtcttttaa ggttttgtcc aaactttgca
     5341 gcgtattttg tacgtctccg tagataggcg attgaggcga tacgccttgc agggttatgc
     5401 gcaactcttt cagagtttgg ttcagttcgt tcgggatgtt ttgcgtctgc ggattgccga
     5461 ccagtttgtc aatggagctt agggcggcat tggcggattt gagtgcggac ttgagttcgg
     5521 cgagcgagcc gttcaattcg gcaacggttt tatccaatgg cagattgttg aatttgtcca
     5581 gcaaatccgc caatttggcc tgcaagtcgt ccaaaccgcc gccccgtgtg gcgatgacgg
     5641 tatcgcctgc ataaacggta tgcggtcgca gcttgggcga ggcggaaggc tgatcgttca
     5701 actcaatcat tttgccgccg gtcagcaggt tgttgctgga gatggtggcg gtcaggcctt
     5761 tgtttaaggc cgtctggaat tgttgtttcc aatgctcttt gctttgctcg tcggcattga
     5821 tttccaaacg ggaaggctcg atgcggatgc gtacgggaat ccagccgttt tcaaacaggt
     5881 gcaggctgtc attgcggtca aaataaggga catcggaaac catgccgaca ttcagccctt
     5941 tgtattcgac aggcgaaccg acggtcagtc cgcgcacgga ttgtttgaaa aacgcggtgt
     6001 agtacagcga gcggtcgtca ggcaggttgg cgatttcgct gcggctgtcg taaagcgtga
     6061 agctgtcctc gcttttgacg tttttactgt ttttggtttt cggcgaatca aatgaaatcg
     6121 cgcctgacag cagggcaggc agaggggcgg aattgagttt gatgccgctg cctgtggttt
     6181 cgatattgat gccgctttcc agccaaaaac ggctggcgga atgaatcagt ttgtcgttgg
     6241 ggctttggat gaagatggtg taatgcacgc tttggtcgga cgggtcgaaa tgcgcgcttt
     6301 cgatttgccc gaccataaag ttttcataca aaacagggct gttgacgttg aggatgcggt
     6361 cgtttttacc aatcaaattc aagcgcagcc cgctttgccc gatggcggta acgggcggaa
     6421 tgtcctgcac ttggaacacg tcttttgcct cgccgctttt gccgggtgta aaagcgatgt
     6481 acgaacccga aagcagcgta cccaaaccgg ttacgccgct ttggtcgata cgcggcttga
     6541 ccacccagaa ctgggtatcg ctgcggatga ggccggatac gtccgcattg agttgggcag
     6601 taacttccac gcctttttgg tcgtcgcgca gtttgattcg ggtaacgcgt ccgacatcga
     6661 tgctcaatac cttaatgacc gtattgttga cttcgatgcc ttccgcgctg tccatcaaga
     6721 gcgtaaccac aggccccctg ttgcggattt ccttaaccca aagccagccg ccggcaatca
     6781 gcgcgatcag cgggaccagc cagacggcgg agaggaaggt gttgtttttg cggacgcgtg
     6841 cttgagcgtg tccgtttgga ggagggctgt tgtcagtcat atttttccgt ttcgttgaaa
     6901 gcaatgccgt ctgaagcgcg tttgtcccaa agcaggcgcg ggtcgaaata ataggcggac
     6961 agcatcgtca gaatcacgac caggcagaaa tagactgccg cactgcccgg aatgacgcgc
     7021 gcggcataag tgtggaacga acacatcaaa ataataatca caaaaatatc aatcatcgac
     7081 cagcggccga ccgcttcggt gatgcggtag aggtgcgaca atttctttgc gcccgccggc
     7141 aaagcgaacc gtgccgccgc aatcaaaacc gacattgccg caatcttcag caccggcacc
     7201 aaaatactcg cgctgaaaat aaccgccgca atcagcctgt cgccctcgtc ccacatataa
     7261 gcgatgccgc taaagatggt gttggcctcc gtggcggcag gattggacga aatcataatc
     7321 ggcaggatat tggcagggaa atacaaaaca accgccgccg tcagaaacgc cgacgaaata
     7381 ctcagacttt tcggccgtcg gcggtacagt tccgcgccgc acaccccgca gggggattcg
     7441 gcactgtcgc ggaaatacag gcagcggctg caacaggttt tgccttccga tgccgtctga
     7501 accgcattat tccccgtcag ccgcccgatt tggaaataca cccaatgctg gggaaccgat
     7561 accgaagtcc gaatcagcat aaccgacagc gcgaacatca gataaaacgc cggcccgaag
     7621 cgaacctttg ccacagacga gagcttgata tacgccacca gagtggaaac aaaaaacaca
     7681 tccaccatca tcgcctgcct caagcgcacc atcacacgcg ttgccaaacg cagcgcagga
     7741 tacgcctgtt tccgtatcag cgcggcatag acatacaggc acagcagcag aaacagaacc
     7801 ggcgcgccga aagtcagcac aaacatcact tcggccaaaa aaccataatc ctgaaacacc
     7861 atcaggcgca tcatctcggg cagcgaaagg acggatgccg cacccggtat cccgacctcg
     7921 atatacgtca tactgtaagc aaacgccatc aaaatcagcg aagccgccgc ataggcgggc
     7981 ggaccggaaa aagggtggct gcccaccctg aagagtttgt gtccgcaacg gggacagaac
     8041 gccgcctctc ccttgtccaa ctggggcacg tctgtacggc agccgcattc cgggcaatct
     8101 accgtatgcg gcggcagggc ttcgtcgcgg cagttgccgc gaaggctgtg ggggatattt
     8161 tcggcaaacg gcttcatcgg gaaagtggca aatataaatg gcggcattat aaaggaaaaa
     8221 aagcgataca cccaatcagg atgcaaaacc ggcaggttcg gcagtatgaa caaacccttg
     8281 gtgtccggca aaaatatttt ttgagcggct gcccctccgg ccgacaagcc tatttgataa
     8341 aaccccgatt ttgtaaataa tcataaaata cccgcttaaa aatataacgc aaatgatatt
     8401 ttttcgtaaa atatgtgata ataaatttcg tttatgattc tttattattt ttgtatgcgg
     8461 gcaagtccgg attttgcccg caccgcatcc ttatccctta ccgacaaagg agaacttatg
     8521 agcgaaaccg aaaaccaagc cctgacgttt gccaaacgcc tgaaggcgga taccacggcg
     8581 gttcacgaca gcgtggataa cctcgttatg tctgtccaac cgtttgtcag caaagaaaac
     8641 tacatcaaat ttttgaaact gcaatccgtg ttccacaaag cggttgatca tatctataaa
     8701 gaccccgagt tgaacaaagc catttccgag ctggaataca tggcgcgata cgatgccgta
     8761 acgcaagact tgaaagactt gggcgaagag ccttacaaat tcgacaaaga attgccacac
     8821 gaaaccggca acaaagccgt cggctggctt tattgcgccg aaggatccaa tttgggcgcg
     8881 gcatttttgt tcaaacacgc gcaaaagctg gattacaccg gcgaacacgg cgcgcgccac
     8941 ctcgctccgc atccggacgg gcgcggcaaa cactggcgcg ccttcgtcga gcacctgaat
     9001 gccctgaact taactccgga agcagaagca gaagccattc aaggcgcgca ggaagcgttt
     9061 gctttctata aagtgattct gcgtgaaacc ttcggtttgc cggaaggaac agaagccccc
     9121 gaaggcatga tgccgcacag acattaaaaa tggggctgta ctagattatc cctaaattcc
     9181 acaccgatcc cgcaggattt ttagctgccg gggcggtgtg ccg
//
LOCUS       DAWTCI010000079         9144 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWTCI010000079 DAWTCI010000000
VERSION     DAWTCI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.079"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            64..372
                     /locus_tag="WM481_001795"
                     /pseudo
     CDS             64..372
                     /locus_tag="WM481_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
     gene            complement(447..2501)
                     /gene="metG"
                     /locus_tag="WM481_001796"
     CDS             complement(447..2501)
                     /gene="metG"
                     /locus_tag="WM481_001796"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFA9081057.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVRKEHLADFTGFFIGYDNYYSTHSTENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGGETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRPKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWKNTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTASAPVE
                     EGKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     SYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(2617..4455)
                     /gene="glmS"
                     /locus_tag="WM481_001797"
     CDS             complement(2617..4455)
                     /gene="glmS"
                     /locus_tag="WM481_001797"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFA9081058.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVYSDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSPASLEPGPHSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSVCNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVVLFGLAVTLAKVRGVVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNAAEGVHVI
                     RAPRHVGELSPVVHTVPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            4727..5233
                     /locus_tag="WM481_001798"
                     /pseudo
     CDS             4727..5233
                     /locus_tag="WM481_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5537..6862)
                     /locus_tag="WM481_001799"
     CDS             complement(5537..6862)
                     /locus_tag="WM481_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFA9081059.1"
                     /translation="MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKRFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIY
                     GIPDDFISVPLPAGLRGGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSGNEGPVGALGTPLMGEYAGAIDRHYITLGAPLFVATAHPVTRKVLNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            7095..7914
                     /locus_tag="WM481_001800"
                     /pseudo
     CDS             7095..7914
                     /locus_tag="WM481_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
     gene            7911..9077
                     /locus_tag="WM481_001801"
     CDS             7911..9077
                     /locus_tag="WM481_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFA9081060.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VGIAVNDNACEPMNLTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVADSGFKDTANEADLEKLPQPLADYKAYVQGEV
                     KELAAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDACEDDFK
                     DGAKDAGFTGFHRIEHALWVEKDVSGVKETAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEAAGSKISGEEDRYSHTDLSDFQANADGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLSEADRKALQAPINALAEDLAQLRGILGLK"
BASE COUNT         1894 a         2282 c         2838 g         2130 t
ORIGIN      
        1 gggggatgcc acgccggcag cgccgttgtt ttccggccat ttgccgtgat tcgggtaatg
       61 tccggcgaaa acgaattgcg gcagatgaag ccgggtgcgg ttttgatcaa ttgcgggcgc
      121 ggcgggctgg cggatgaaaa cgcgctgcct gccgcactca aatacgggca gatcggcggg
      181 gcgggtgtcg atgttttgac ggaggaaccg ccgaggggcg gcaatccctt gctgaatgca
      241 cgattaccca atctgattgt tacgccgcat accgcgtggg cgagccgcga ggctttggat
      301 cggttgtttg aaatattgtt ggcgaacatt cacgcctttg tgaaaggcga ggcgcaaaac
      361 cgcgtggttt gaacctgtcg ggattgcgga aaaaaacgcc gtctgaacgc ccaaggggtt
      421 cagacggcgt tttttgagat ttctgcctaa ccgactttgt cgcccggctg agcgcccgca
      481 tccacgtcaa gcagcttcag tttgccgtct gccgtggcgg cggagaggat cataccttcg
      541 gatacgccga attttgccat tttgcgtggg gcgaagttgg cgactgcgat gaccatgcga
      601 ccgttcaatt cggcaggatt tgggtaagac gcggcgatgc cggagaagat gatgcgtttt
      661 tcaaaaccga aatcgaggtc gaatttcaaa agtttggtgc tgccttcgac ggcttcgcag
      721 ttcaatactt tggcgacgcg catatcgatt ttcataaagt cgtcgaagcc cgcctgttcg
      781 gcgacttttt cgtatttacc ctcttcaacg ggcgcggatg ccgtctgaat gctttgtttg
      841 ttggcttcga tcaaatcgtc cacttgtttt tgctccactc gttgcattaa atgttcgtat
      901 ttgttgatgg cgtgttcgcc tagggtttcg cgcgtgttct tccaggtaat cgcgtccaga
      961 ttgaggaaac gcgcggcgtt ggcggcggtt tgcggcaaca cgggggcgag gtaggcggtc
     1021 agcatggtga aggcgttgat gagttcgctg catacttcgt gcaggcgttc gtcttgacct
     1081 tcttgtttgg cgagctccca cggtttgttg gcatcgacgt attcgttgac aatgtctgcc
     1141 aatgccatga tgtcgcgcag ggcgcgggcg tattcgcggt tttcgtattg ctcggcgatg
     1201 gtgtcgcttt cggcggcgag ttttgccagc aatgcgctgc ctgaaacgtc tttcggacga
     1261 ccttcaaagc gtttggcgat gaaacccgaa gcgcgggcgg cgatgttgac gtatttgccg
     1321 acaaggtcgc tgttgacgcg cgaaataaag tcttgcaggt tcaaatcgat gtcttcgatt
     1381 ttgctgttga gcttggcggc gatgtagtag cgcatccact cggggttcag gccttgttcc
     1441 aaataggatt tggcggtgat gaaggtgccg cgcgatttgg acattttttg tccgtccacg
     1501 gtcaaaaagc cgtgtgcgta cacgccggtc ggggcgcggt ggccggagaa atgcagcatg
     1561 gcgggccaga acagggcgtg gaaatagagg atgtctttgc cgatgaagtg gtacatctcg
     1621 gtttggctgt cggctttgaa gtattcgtcg aaatcgatgc cgatgcggtc gcacaggttt
     1681 ttaaacgacg ccatatagcc gacgggcgcg tccagccaaa cgtagaagta tttgcccggc
     1741 gcgtcgggga tttcaaaacc gaaatacggc gcgtcgcggg aaatgtccca gtcggacagg
     1801 gtggtttctc cgccttcgcc cagccattcc ttcattttgt tgagggcttc gggttgcaga
     1861 tggggcttgc cgtcgtgcgg gttgttgccg gaagtccatg ctttgaggaa gtcggcgcat
     1921 tcgcccagtt tgaagaagaa gtgttcggat tcgcgcaatt cgggtttcgc gccggaaacg
     1981 gcggaatacg ggttgatcag ttcggtcggg gaataggtcg tgccgcagac ttcgcagttg
     2041 tcgccgtatt ggtcttgggc gtggcatttc gggcattcgc ctttgacgaa gcggtcgggc
     2101 aggaacattt gtttttcggg gtcgaaaagc tgctcgatga cgcggctttc gattttgccg
     2161 ttggctttca gcgcgcggta aatgtcttgg gaaaactgtt tgttttcagt ggaatgggtg
     2221 ctgtaataat tgtcgtagcc gatgaaaaag ccggtaaagt cggcaaggtg ctctttgcgc
     2281 actttggcaa tcatgtcttc gggcgcgatg ccttgttttt gcgcggcaag cataacgggc
     2341 gtgccgtggg tgtcgtcggc gcagcagtag tagcattcgt ggccgcgcag tttttgaaag
     2401 cgcacccaaa cgtcggtttg gatgtgttcg accatgtggc cgaggtggat gctgccgttg
     2461 gcatagggca gggcggaggt aactaagatt ttgcgtgtca tattgtgctt tgcaaacaat
     2521 gggtaaaggc ggattatacc gcaaatcaaa cggggaaatg ccgtctgaag cctgaaaaat
     2581 cgggcttcag acggcatttt tgccaaccgg cgggatttat tcgacggtta cggatttcgc
     2641 caggttgcgc ggcttgtcca catccgtgcc gcgcgcgagg gcggtgtggt aggcgaggag
     2701 ctgcacgggg acggtatgca cgacggggga cagttcgccg acgtggcgag gtgcgcggat
     2761 aacgtgcacg ccttcggcgg cattaaaatt gctgtcgagg tcggcaaaga cgaaaagttc
     2821 gccgccgcgc gcgccgactt cctgcatatt ggctttgact ttgtccaaca ggctgtcgtt
     2881 gggcgcgatg acgacgacgg gcatgttttc gtccaccaag gcaagcggcc cgtgtttcag
     2941 ttcgccggca ggataggctt cggcgtggat gtaggtgatt tcctttagct tcaacgcacc
     3001 ttcaagggca atcgggtaat ggatgccgcg ccccaaaaac agcgcgctgg ttttcttggc
     3061 aaactgttgc gcccaagcgg caatttgcgg ttcgaggttc aaagcgtgct gcacgctgcc
     3121 gggaagctgg cggagttctt cggtgtaacg tgcttcatct tcgccggata ccacgccgcg
     3181 cactttcgcc agcgttaccg ccaaaccgaa cagcacgacc agttgcgtgg taaatgcttt
     3241 agtcgaggcg acgccgattt ccgcaccggc acgggtataa agcacaaggc tgctttcgcg
     3301 cggcagggcg gattccatca cgttgcagac ggagaggctg tggcggtgtc ccaaagattt
     3361 ggcgtatttc aaagcctcca tcgtgtccag cgtttcgccg gattgggaaa tggtaatgac
     3421 cagttggtcg gggtcggcaa tcacgctgcg gtaccggtat tcgcttgcga tttcgacgtc
     3481 ggccgggatt ttggcgatgg attccaacca atatttggcg gtcagcgcgg cgtaatagga
     3541 cgtgccgcag gcaaggattt tgacgctgcg gatgctttca aacacgcttt tggcgttttt
     3601 gccgaagttt tcggggatga atccgccgtc gaggaaaact tccgccgtgt ccgcaatcgc
     3661 gcgcggctgc tcgtggattt ctttttgcat aaagtggctg tgcggacccg gttccaaaga
     3721 ggcgggcgag agttcggata ccttgacttt gcgttcggca ggcaggccgt ttttatcggt
     3781 cagccgtttg atgccgtctg aagccagcag cgcgatgtcg ccgtcttcga ggtacgccac
     3841 gcggcgcgta aaggcgatga cggcggatac gtccgaagcg ataaaggttt catcgtcgcc
     3901 caaagcgacc aaaagcgggc agcccatacg cgccacaact aattcatcag gcttgtcttg
     3961 ggcaataacc gcgatggcgt atgcgccgtg gaaacgtttg accgcttcct gcacggcttc
     4021 aaacaatttg ccgccgtttt gcgcgtattc gtgattgatg ctgtgtgcga tgacttcggt
     4081 atccgtttgc gattcaaacc ggtatcccaa accttccaag cgtttgcgtt cgctttcaaa
     4141 gttttcgatg atgccgttgt ggacgacggc aatcataccg ccgctgatgt gcgggtgggc
     4201 gttcggctcg gtaacgccgc cgtgtgtcgc ccagcgcgta tgcccgatgc cgatgccgcc
     4261 gctgatgcct ttttcgcgtg ccgcgtcctc cataagctgc acgcgtccga cgcggcgcac
     4321 gcgcttgatt ttgccgtctg aatacacagc aatgcctgat gagtcataac cccggtattc
     4381 gaggcgtttg aggccgtcgg tcagaaaatc gacgacgttg tgatgggcgc ggatggcgcc
     4441 gacgataccg cacataaact gttccttagt atccggttga aaaaaaagat gcggacggct
     4501 tccgtaccgc accttccgtt tcggattata aaccgcctcc cgcgccggaa aacagcaaaa
     4561 tgccgtctga aggcttgggc ttgctcaaaa aagggaggga tttccccgcc tgaccgggat
     4621 gggcgttcag acggcattgc ctgccgccgg tttatagttt ttgcaaatca atattaacaa
     4681 gctgaaaaaa aacaatatac ccgcccggtc ttaatgttaa cggagtatgg aaatgaaaca
     4741 aatgcttttg gccgtcggcg tggcggcggt gttggcgggc tgcggcaagg atgccggcgg
     4801 ttacgagggt tattggcgcg aaaagtcgga caaaaaagag ggcgtgattg ccgtcaaaaa
     4861 agaaaaaggc aattacttcc ttaataaaat caacgtgttt acaggcaagg aggagtcttt
     4921 gcttttgtct gaaaaagacg gcgcgctttc gataaacacg gggatagggg aaatcccgat
     4981 caaactttcc gacgacggga aagagctgta tgtcgaacgc aggcggtatg tgaaaaccga
     5041 tgcggcgatg aaggacaaaa tcatcgccca ccagaaaaag tgcggacaaa cggcacaggc
     5101 atacctcgac gcgcgaaatg cgttgccgtc aaaccaaacg tatcagcagc gtcaggcggc
     5161 gatcgagcaa ttgaaacggc ggtttgaagc cgagtttgac gaattggaaa aagaaatcaa
     5221 atgcaacggc agaaccccgg cattgttgct ttggcagggg acaaccggga ggatgccgcc
     5281 gtccgaatcg gacgcgtggt ttccataccg gtacgggcgg gcgggaatgt ccgccttttt
     5341 ttgttcggat gcgtttgaat acccgtccga ttctgtccgt ttgcaagggg gatttccgtt
     5401 cgggcggaaa tccattccgt caaataagaa aggcgttttg tccggccggc cggcttgtgc
     5461 ctgcggtatc gcaaaacttc gccttaatgc gcccgattgg ctagggacgg gcttcggacg
     5521 gcattgtttt gccggttcac gggcggtatt cgggcttcat gccgttgggc aggagctgcc
     5581 agacgtatcc cgtggttttc tgtttgccgg caagttcgcc ggcttcgtcg ccgtaacccc
     5641 aaaaataatc cacgcgcacc gcgcctttga tcgcgctgcc tgtatcctgc gccataatca
     5701 ggcggttgag gactttgcgg gtaaccggat gggcggtggc gacaaataag ggcgcgccca
     5761 aggtaatgta gtgccggtcg attgcgccgg cgtattcccc catcagcggc gtgcccagtg
     5821 cgccgacggg gccctcattg ccgcttccgg caagctcgcg gaaaaagata tagctggggt
     5881 tttgccccaa aacttcggcg aggcgttgcg gattttgccg catataggct ttgatgccct
     5941 gcatggaggt ttgcccgagc ttgaggtagc ctttgtccgc catatagcgt ccgatggaaa
     6001 catacggatg ctcgtttttg tcggcatagc cgatgcggat gtatttgccg gacggggttt
     6061 tcagacggcc cgagccttgt atgtgcatga aaaaaagttc gacggggtct tcggcgtaac
     6121 cgagtatcgg ggctttgccg tcaagcgcgc cgccgttgat ttggtttcgc gtgtggtagg
     6181 ggaggaagcg gcttccttca aacctgccct tgattgccgt tgtgcgcgcg gtaatgggga
     6241 atcgggaaag gtcggcggta tgcgtgccgc cggcattgtc gattgtgccg ctgttttttc
     6301 ccgtctgcct gatgcggaca agggctttcc cgccccgcaa accggcaggc agcgggacgg
     6361 agataaaatc gtcgggaata ccgtaaatcg ggaagcgggc ccgttccgtc cgcctgccgt
     6421 cgcccttcag caccggctcg taatagccgg taaccgtacc tgcaaggctt ccgttgcctg
     6481 caacctgcca cggcgtgaaa tagcgttcaa aaaaccgctt tgcctgaaag gaatgcacgg
     6541 gggtttgaaa ggcttgggcg cacacatcct gccagccttg gcggtttttc aaattggcgc
     6601 agccgaggcg gaaggattgc aggcttttgg caaaatcctg cgccgcccag tggggcatgg
     6661 acaggtgcgg cacaacggta tagacggccc cgccgccggc aaccgtcgtt ccggcggggt
     6721 cggggatgcc ggccggccgg tccgggccgt tgatgacgga tgtgtcgggt tgcggaaagg
     6781 tttggatgct cctgctttgg caggcggcga ggatggcggc ggcgatgccg tacagggcgg
     6841 agcggagcag gtgttttttc ataatggaca atgttgtcgt cagtaataaa aaaagatggt
     6901 ttcgggcggc gttgcggtag ccggggagag gggattttaa cacagggcgc agccgcagcc
     6961 tgcggaactt tccgccacgc ggcaatgcag ataaaaataa cttgcatttg tatttgcaag
     7021 taatgaaaat attccgataa tatattatta atcatccttg ttcattcgcg tttacgctgg
     7081 tcgctttttt aattatgttg cgcgagggta tcgaagccgc gctcatcgtc ggcatcgttg
     7141 ccggtttttt gaaacagtcc ggacattcaa aaactgatgc ctaaggtctg gttcggggtc
     7201 gtccttgctt ctttgatgtg tttgggcatc ggatacggca tccattcggc aacgggcgag
     7261 attccccgga agcagcagga gttcgtcgtc ggcattatcg gtttggttgc cgtcgcgatg
     7321 ctgacttgtg tggttttatg gatgaaaaag gcggcgcgtt cgatgaagcg gcagcttcgg
     7381 gattctgtac aggcggcttt gaaccgtggc agcggtcaag gatgggcctt ggtcggtatg
     7441 gcgtttcttg ccgtggcgcg cgaaggtttg gagagcgttt ttttcctgct tgccgtattc
     7501 aaacagagcc cgacgtggca gatgccggcc ggcgcggtag cgggggtttt ggctgccgcc
     7561 gtgattggcg cgttgattta tcagggcggg atgcgcctga atctggcgaa gtttttccgt
     7621 tggacggggg cgtttctgat tatcgttgcc gccggtctgc ttgccggctc gctgcgtgcg
     7681 ctgcatgagg caggcatttg gaacgcgctt caggacattg tgttggaccc atcaaaatat
     7741 ttgcacgaag acagtccgtt gggtgtgctg ctcggcggat ttttcggcta taccgaccat
     7801 ccggcgcagg gctgggcctt ggtttggctg ctgtacctta ttcccgtcat gatttggttt
     7861 ttgcgcggca gcaggccgtc tgaaacttta acccgtaaag aggaattgaa atgagaaaat
     7921 tcaatttgac tgcattgtcc gtgatgcttg ccttgggttt gaccgcgtgc cagccgccgg
     7981 aggcggagaa agccgcgccg gccgcgtccg gtgagaccca atccgccaac gaaggcggtt
     8041 cggtcggtat cgccgtcaac gacaatgcct gcgaaccgat gaatctgacc gtgccgagcg
     8101 gacaggttgt gttcaatatt aaaaacaaca gcggccgcaa gctcgaatgg gaaatcctga
     8161 agggcgtgat ggtggtggac gaacgcgaaa atatcgcccc ggggctttcc gacaaaatga
     8221 ccgtaaccct gctgccgggg gaatacgaaa tgacctgcgg ccttttgacc aatccgcgcg
     8281 gcaagctggt ggtagccgac agcggcttta aagacaccgc caacgaagcg gatttggaaa
     8341 aactgcccca accgctcgcc gactataaag cctacgttca aggcgaggtt aaagagctgg
     8401 cggcgaaaac caaaaccttt accgaagccg tcaaagcagg cgacattgaa aaggcgaaat
     8461 ccctgtttgc cgacacccgc gtccattacg aacgcatcga accgattgcc gagcttttca
     8521 gcgaactcga ccccgtcatc gatgcgtgtg aagacgactt caaagacggt gcgaaagatg
     8581 ccgggtttac cggcttccac cgtatcgaac acgccctttg ggtggaaaaa gacgtatccg
     8641 gcgtgaagga aaccgcggcc aaactgatga ccgatgtcga agccctgcaa aaagaaatcg
     8701 acgcattggc gttccctccg ggcaaagtgg tcggcggcgc gtccgaactg attgaagaag
     8761 cggcgggcag taaaatcagc ggcgaagaag accgttacag ccacaccgat ttgagcgact
     8821 tccaagctaa tgcggacgga tctaaaaaaa tcgtcgattt gttccgtccg ttgattgagg
     8881 ccaaaaacaa agccttgttg gaaaaaaccg ataccaactt caaacaggtc aacgaaattc
     8941 tggcgaaata ccgcaccaaa gacggttttg aaacctacga caagctgagc gaagccgacc
     9001 gcaaagcatt acaggctcct attaacgcgc ttgccgaaga ccttgcccaa cttcgcggca
     9061 tactcggctt gaaataagcc gcaagcgttc agacggcatt tggcggcaga taccgtctga
     9121 agttttatag cggattaaca aaaa
//
LOCUS       DAWTCI010000080         8715 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWTCI010000080 DAWTCI010000000
VERSION     DAWTCI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.080"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..258)
                     /locus_tag="WM481_001802"
     CDS             complement(<1..258)
                     /locus_tag="WM481_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081061.1"
                     /translation="MNTKTELQKLLEEDISTLKETLIRVDALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYS"
     gene            complement(320..2944)
                     /gene="alaS"
                     /locus_tag="WM481_001803"
     CDS             complement(320..2944)
                     /gene="alaS"
                     /locus_tag="WM481_001803"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFA9081062.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGKEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVQEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGGQVVELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFAGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSITAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTDAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALRETVTDLTGKSDNA
                     VILLAAVNEGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDTD
                     KLPEMLASAEGWLCQKLS"
     gene            3148..4278
                     /locus_tag="WM481_001804"
     CDS             3148..4278
                     /locus_tag="WM481_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9081063.1"
                     /translation="MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSMIPNYKHLNPEMMRLMDGVDPDHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFNPEYTFKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARDLMEDE
                     FKNDNTIFPSGEDLKNSFIMVPIRPAALKFMVRQWQDVKAGK"
     gene            complement(4319..5221)
                     /locus_tag="WM481_001805"
     CDS             complement(4319..5221)
                     /locus_tag="WM481_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9081064.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTVMNVLIKEASAKFALGSGELVF
                     WRMLFSTVTLGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLTTGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQEPAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPIAFKQRLQALFRQR"
     gene            5360..5857
                     /locus_tag="WM481_001806"
     CDS             5360..5857
                     /locus_tag="WM481_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HFA9081065.1"
                     /translation="MAGMKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAP
                     AKGFRLAPSHWADVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDS
                     MYEIYLRSAVDSQRGEINTEQSKLYIENALRGWQQRWKNMDAKPDNPAFTNFLMEVMK
                     MQPLK"
     gene            complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="WM481_001807"
     CDS             complement(5919..6824)
                     /gene="mtrA"
                     /locus_tag="WM481_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFA9081066.1"
                     /translation="MDILDKLVDLAQLTGSADVQCLLGGQWSVRHETLQCEGLVHIVT
                     AGSGYLCIDGETSPRPVGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQNGTFMVKQC
                     GNGLDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESEKPLTGTVS
                     VVNALPSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNIDKM
                     VAAANMSRAQLMRRFKSQVGLSPHAFVNHIRLQKGALLLKKTPDSVLEVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            6953..7288
                     /locus_tag="WM481_001808"
     CDS             6953..7288
                     /locus_tag="WM481_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFA9081067.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            7467..7889
                     /locus_tag="WM481_001809"
     CDS             7467..7889
                     /locus_tag="WM481_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081068.1"
                     /translation="MSAVFIAPYLTAFHEQEKIFEYADLTVTAPNRSGRAIKLEADGR
                     QYRLSCYGFDSLCTGGNIGRAIRARQVKIVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FSRTEDRLVEVLAQPAVFSLKPGILLLLPAIFLRLKKM"
     gene            7995..8558
                     /gene="pgsA"
                     /locus_tag="WM481_001810"
     CDS             7995..8558
                     /gene="pgsA"
                     /locus_tag="WM481_001810"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFA9081069.1"
                     /translation="MPWNIPIFLTWLRVLLIPVLIVLFYLPFSWFSEEAVNVAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRSSVAVATVGKFKTAAQMLAIFFLLLNFPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            8639..8714
                     /locus_tag="WM481_001811"
     tRNA            8639..8714
                     /locus_tag="WM481_001811"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8672..8674,aa:Gly,seq:gcc)
BASE COUNT         2049 a         2156 c         2331 g         2179 t
ORIGIN      
        1 actataaaaa ttaactttat tctctaaggt tgacaatttt tcaaatacca aacttgtatc
       61 aaggatgggc aattctatag ttaaacctat ttcttttgcg agaatattta attgtttgtc
      121 aattaaccat cgacggacaa taggagttgc aatgctacgt acatatcttg gaggaagagc
      181 atctacacga attagtgttt cttttaaagt tgatatatcc tcttctaaaa gtttttgtaa
      241 ctctgtttta gtattcattc taacaatcca aaatataagc catttgaaca agactcaaat
      301 ggcttattaa gatttaaatt taacttaatt tctgacaaag ccaaccttcc gcgcttgcca
      361 gcatttcggg caatttgtcg gtatccgtac cgccggcttg cgccaagtcg ggacggccgc
      421 cgcctttgcc gccgacttgt tcggctgcaa atttaaccag atctcctgct ttgacttttc
      481 cggtcagggc tttggatacg ccggcgcaca gggagacttt accctcgttt actgccgcta
      541 aaagaatcac ggcgttgtcg gattttccgg tcaggtcggt tacggtttcg cgcagggcgg
      601 ctgcgtcggc ttcgatttgt gcggcaacga gtttggccgc gcccaggtct tttgcgtcgt
      661 ccaagagttt ggcgcctgcg tggacggcga gttcggcttt ggcgcgtgcc aattcttttt
      721 ccaatgcttt ggcgtgtgcc gcgcctgctt ggattttcgc cagtacgtct ttttcggttt
      781 gggctttggt ttcggcaatg atgtctttta ccaaacgctc ttgctcttgc gcccatttga
      841 gtgcgttcag gccggtgatg gcttcgatac ggcgtacgcc tgcggcaata ccgccttcgc
      901 tgatgatttt gaagaggccg atgtcgccgg tgcgtgaaac gtgtgtgccg ccgcacaatt
      961 cggtagagaa accgcccatt tgcagtacgc gtacttcgtc gccgtatttt tcgccgaaga
     1021 gcatcatggc gtcggttttt tgcgcgtctt ccatgctcat aatggctgcg ttgacggcaa
     1081 cgttggccaa gatggcttcg ttgactcggc gttctacttc ggcgatttct tcggcagtta
     1141 ccgcttgggg atgggaaatg tcgaaacggg tggattcggc ggtaaccaaa gagccttttt
     1201 gttcgacgtg tccgcccaat acgtcgcgca gggctttgtg catcaggtgg gtcgcgctgt
     1261 ggttgcgcat attggcattg cggatttcgt catccacttt ggcggtaatg ctgtcgccga
     1321 ctttcagacg gcctgaagtt tgtacgccga attgtccgaa tacggccgct ttgatttttt
     1381 gggtatcgcg tacttcaaag cggttttcgc ctgcgaagat atagccgacg tcgccgactt
     1441 ggccgccgga ttctgcatag aacggggtaa agtcgataac gacggcaccg ctgtcgcctt
     1501 cgttcaattc gacgacttgc ccgccgtctt tgtagagggc gaggactttg gattcggttt
     1561 ggcgttcgct ataaccttta aactcggtgt cttgaccgtc gtaaggcagt tgggcgttgg
     1621 ctttgaagct ttgggcggcg cgtgcgcgtg cgcgttgggc ttccatttcg cgctcgaagc
     1681 ctgcttcgtc cggttcgata ttgcgttcgc ggcagatgtc ggcagtcagg tcgtatggga
     1741 agccgtaggt atcgtagagt ttgaagataa tttcgccgcc gagtgttttg ccgcctttgg
     1801 ccagcgcgtt ttccaacaaa gccataccgg tttccagagt ttgggcaaaa cggctttctt
     1861 cgtttttcaa agcttcttcg atttgcgcct gtttttcttt caattcagga tacgcgccgc
     1921 ccatctcttg aaccaaatcg gcaacgagtt tgtggaagaa cggtttgctt tgacccagtt
     1981 tgtaaccgtg gcgcacggcg cggcggatga tgcggcgcaa tacgtagccg cgtccttcgt
     2041 tggaaggcaa tacgccgtct gcaatcagga atgagcagga gcggatgtgg tcggcgataa
     2101 ctttcaggct gggttcttcc atactgaacg gcgcgccggt ttcgcgggca acggctttga
     2161 gcaggtcttg gaacaggtcg atttcgtagt tgctgtggac gtgctgcatc acggcggcca
     2221 tacgttccaa gcccataccg gtatcgacgg acggcttggg cagcggattc atattgcctt
     2281 gttcgtcgcg gttgaactgc atgaatacgc agttccaaat ttcaatccag cggtcgccgt
     2341 cttcttcggg gctgccggga atgccgcccc agatttcttt gccgtggtcg tagaaaattt
     2401 cggagcaggg gccgcaaggg ccggtgtcgc ccatttgcca gaagttgtcg gacgcgtatt
     2461 tcgcgccttt gttgtcgccg atgcggacga tgcgttcaga cggcataccg atttcgttca
     2521 accagatgtt gtaggcttcg tcgtcttctg cgtaaacggt cgccaagagt ttgtctttgg
     2581 ggatgttgag ccattcgggg gaagtgagaa actcccaagc gaagtggatc gcgtcgcgtt
     2641 tgaagtaatc gccgaaggag aagttgccca tcatttcaaa gaaggtgtgg tggcgggcgg
     2701 tgtagccgac gttttccaag tcgttgtgct tgccgcctgc gcgtacgcat ttttgcgcgg
     2761 tggtggcgcg gctgtacggg cgtttgtcga aacctaaaaa tacgtcttta aactggttca
     2821 tgcctgcgtt ggtaaacagc agggtcgggt cgtcgtgcgg cacgaggctg gaagagcgga
     2881 cgacggtgtg gcctttggtt tcaaaaaatt ttaggaattt ttggcgcagt tcggaggttt
     2941 tcataatttt ttcaatgtct ctcaaatgtc ttgtcatggt aaaagcaggg aaaacgaacg
     3001 gcggtatatt accgcaaatc cctgtttcta gctatggaag cggcggcttt cagacggcat
     3061 tgcgggattt tgaatgccgt ctgaagccct gttaccaata tcggctataa tggccgcttt
     3121 ctccaacccg atatgcaagg aatgataatg gtcaaacatc tgccactcgc cgtcctgact
     3181 gctttgctgc ttgcagcgtg cggcggttcg gacaaaccgc ctgccgaaaa accggcaccg
     3241 gcggaaaacc gaaacgtatt gaaaatttat aactggtcgg aatacgtcga tccggaaacc
     3301 gttgccgatt ttgaaaagaa aaacggcatc aaggttactt atgatgtgta cgacagtgat
     3361 gaaacgctgg aaagcaaggt gctgaccgga aaatccggtt acgacattgt cgcgccgtcc
     3421 aatgcgtttg tgggcaggca gattaaggca ggtgcgtatc agaaaatcga taagtcgatg
     3481 attcccaatt ataaacatct caaccctgaa atgatgaggc tgatggacgg ggtcgatccc
     3541 gaccacgaat acgccgtgcc gttttattgg gggacaaata ccttcgccat caataccgaa
     3601 cgtgtgaaaa aggctttggg tacggacaag ctgccggaca accagtggga tttggtgttc
     3661 aaccccgaat acacgttcaa actcaaacaa tgcggcatca gctatttgga cagcgcggcg
     3721 gaaatttatc ccatggtgtt gaactatttg ggcaaaaacc cgaacagcag caatacggaa
     3781 gacatcaggg aggcaaccgc cctgctcaag aaaaaccgcc ccaatatcaa acgctttact
     3841 tcgtccggct ttatcgatga tttggcgcgc ggcgatacct gcgtaacaat cggtttcggc
     3901 ggagatttga acatcgccaa acgccgtgcc gaagaagcgg gcggcaagga aaaaatccgc
     3961 gtgatgatgc cgaaagaggg cgtggggatt tgggtggatt ctttcgtgat tccgaaagat
     4021 gcgaaaaacg tcgccaacgc gcacaaatac atcaacgact tcctcgatcc ggaagtgtcg
     4081 gcgaaaaacg gcaatttcgt tacctacgcg ccttcgagca agccggcgcg cgatttgatg
     4141 gaggacgaat ttaaaaacga caatacgatt ttcccgagcg gggaagattt gaaaaacagc
     4201 tttatcatgg tgcctatccg gccggcggca ttgaagttta tggtgcgcca gtggcaggat
     4261 gtgaaggcgg ggaaataaag cccgatatgc cgtctgaagg atgttcggac ggcatttttt
     4321 atctttggcg gaagagggct tgcagccgct gtttgaaggc aatggggcgg atgctgctca
     4381 aaatgccgct gaggataatg atgcacatgc cgagtatttc ctgccagaaa agctcttcgc
     4441 ccagaaaaaa tgcggcagac agggcggaaa agacgacggt catataggaa agcgaggcaa
     4501 ccgtgaattt gtcgccgact ttgtaggcgc gcgtcatcga cagttgggca atcagcgcgg
     4561 acacgccgat gcccgacaga taaaccgccg atggaaagga cagggtgtgc cagccggtca
     4621 gcgtcgccca aaccgacgac atcgccacgc cggttgcgga aaggtaaaac acgacgcgcc
     4681 agccgggttc gcccgccaaa gacagttcgc gcactttcaa atacgcccag ccggacatcg
     4741 cgccgcccgc cagcccggcg agtgccgccg gttcctgacc gctgcggaac gagggattaa
     4801 gcagcaatac cacgccggca aaaccaagga gcagcaccgc ctgcgtgtaa acggaaatcc
     4861 gttctttcaa aatcaggaag gaaaataccg ccaaaaaaat cgacgaggtg taactcaggg
     4921 taacgccggt tgtcaaaggc agatgcgtta ccgcgtaaaa cagcagcagc atcgcccccg
     4981 tcccgaccat actgcggttt aagtggtttt tccaatgggg cgtgcggaag gtgtcgcgcc
     5041 gcaatacggc ggcagcaccg agcgtaacgg ttgaaaacag catgcgccaa aagaccaatt
     5101 cgccgctgcc gagggcaaat tttgccgatg cctctttaat caatacgttc ataacggtga
     5161 agcaggccgc cgccaccagc atccagcccg atcctaaaat gtcttttttt gcggtatcca
     5221 taaacggtcg ttgcgataag gacggtcgga ttgtaaacct tgcggcaagg cttgtggaat
     5281 gtgtttttgc ctgcttctga tgccgaaatt ttatttttct tgccgaacaa tttgttttct
     5341 caaggcaaac ttgattataa tggcgggcat gaaaaaatac cttatccctc tttccattgc
     5401 ggcagtcctt tccggctgcc agtctattta tgtgcccaca ttgacggaaa tccccgtgaa
     5461 tcctatcaat accgtcaaaa cggaagcacc tgcaaaaggt tttcgcctcg ccccttcgca
     5521 ttgggcggat gttgccaaaa tcagcgatga agcgacgcgc ttgggctatc aggtgggtat
     5581 cggtaaaatg accaaggttc aggcggcgca atatctgaac aacttcagaa aacgcctggt
     5641 cggacgcaat gccgtcgatg acagtatgta tgaaatctac ctgcgttcgg cggtagacag
     5701 ccagcgcggc gaaatcaata cggaacagtc caagctgtat atcgagaatg ccttgcgcgg
     5761 ctggcagcag cgttggaaaa atatggatgc caaacccgat aatcccgcat ttaccaactt
     5821 tttgatggaa gtgatgaaga tgcagccctt gaaatgacgc ggtacgcaaa tgccgtctga
     5881 aagctttttc agacggcatt tgcgtttgaa gccccgattt atttttgccc gccttctttc
     5941 cggtattgcc ccggcgaaac gtgatattgc cgtttgaacg ccttgccgaa atgcgtttcc
     6001 gactgaaagc ccaccgacag cgcgacctcc aaaaccgaat ccggggtttt cttcagcagc
     6061 aatgcgcctt tttgcaggcg gatatggttc acaaaggcgt gcgggctgag tccgacttgg
     6121 cttttgaagc ggcgcatcag ttgcgcgcgc gacatattgg cggcggcaac cattttgtca
     6181 atattccatt cgtcttccgg tttgtctatc accttttgga tcaaatgtcc caaacgtttg
     6241 tcctgccaac ctttcaatac gcccgagagt tcgacatcct tatcctgttc gagataggcg
     6301 cgcaggataa gcaccagcag gacggacggt aatgcgttga ccacggaaac cgtccccgtc
     6361 aaaggttttt cgctttccag ttgcagcatt gaaaccacat actgcaaact tggatgggca
     6421 atgttcagaa aaacggtttc cggcagcccg ttcatcaaat cggcgtgggt gtcgtagcgg
     6481 aaacgggcgc aaaacaggct catatccagc ccgttgccgc actgtttgac cataaatgtg
     6541 ccgttttgcc gtatgtccgg ttgtaaactt tctccgtatt ttccgtcgtg gctcaacaca
     6601 tgacccaagc cgcgcgggaa aaatacaata tcgcccgtgc cgaccggacg cggggaagtt
     6661 tcgccgtcga tgcagagata accgctgccc gccgtaacaa tgtgtaccag cccttcgcat
     6721 tgcaaggttt catgccgtac cgaccattgt ccgcccaaaa ggcactgcac atccgcactg
     6781 cccgtcaatt gggcgagatc gaccagtttg tccagaatgt ccataaatct ttttgaacca
     6841 taaaatgaga tgattaaacg agaaacacaa ctgaataatt gcaataatac gcacatcaaa
     6901 aacagatacg caagcgcgta tcagggttaa acgcaagaga aaggaaaaga aaatgtttaa
     6961 agattggaaa gaacataccg cattggttaa aaaatcattc ggcgagctgg gtaaggcgca
     7021 tcctaaaatg ctgcaagcct acggcgcatt ggaacaggcc gccgccgccg aagcactcga
     7081 tgccaaaacg cgcgaactga ttgccatcgc cgttgccatc accacccgtt gcgagagctg
     7141 catcagcgtt cacgccgccg ccgccgccaa agccggtgcg accgacagcg agatcgcagg
     7201 tgcattggca accgccattg ccctgaatgc gggtgctgct tacacttacg ccctgcgtgc
     7261 attggaagcg gttgaaacgc aaaaataatc cgtttcggat aagaaatgcc gtctgaaaat
     7321 atttcagacg gcattttgcc tgttatgctt cccgatattt tgcagacaac atatgcagcc
     7381 gtttttttca gacggcattt gagaaacatg gaacaagtat gaaatatcac cgcttggcat
     7441 tgtttgccgc cataagctgc ctgttgttgt ccgccgtttt tattgcacct tacctgacgg
     7501 catttcacga acaagaaaaa atatttgaat atgcggattt gaccgttacc gcccccaacc
     7561 gcagcggacg ggcaatcaaa ttggaggcgg acgggcggca gtaccggctt tcctgctacg
     7621 ggttcgacag tttgtgcaca ggcggcaata tcggcagagc catcagggcg cggcaggtta
     7681 agattgtttt aagcgaaacc gtcggcaaag gttttttaaa cggcgttctg ttggaatacc
     7741 gcaacagtgg cagtgtctat agcaataaag atttttcccg cacggaagac cgccttgtcg
     7801 aagtgttggc acaacctgcc gttttcagcc tgaaaccggg tattttgctt ttattgcccg
     7861 ccattttcct gcgtttgaaa aaaatgtgaa aacagattgg cggggcgggg gaatgactat
     7921 gcggcaattt tacgttttgg gttatgagga taaccgttat aatcataatt ttacagtccg
     7981 cacagagaaa accgatgcct tggaacatcc ccatcttcct cacatggttg agggtcttgc
     8041 tcattcccgt actgattgtc cttttctacc tgccgttctc atggttttca gaagaagcag
     8101 tcaatgttgc cgccgccgtc atttttgccg ttgccgcctt gaccgactgg tttgacggat
     8161 ttttggcaag gttgtggaaa cagacttccg atttcggcgc attcctcgac cccgtcgccg
     8221 acaagctgat ggttgccgta tcgctgctgc tgctggtcaa actcgaccgg acctatgttt
     8281 tgttcgcgat gattatcatc ggcagggaaa ttaccatttc cgcattgcgc gaatggatgg
     8341 cgcaaatggg aaaaaggagc agcgttgccg tcgccaccgt cggcaagttc aaaaccgccg
     8401 cgcaaatgct ggcgattttc tttttgctgc tgaattttcc cgatttttac ggatttaatc
     8461 ttgttgttat cggcaacata ttgatgttta tcgcatcttt gctgacagtc tggtcgatgc
     8521 tgtattatct gaaaatggcg tggaaagaaa ttgcctgaaa aaaacataaa aatagcttga
     8581 cggtaaaaac ataatccata ataattgcgt cttcttcgat gtcggagagt gaaatcgggc
     8641 gggaatagct cagttggtag agcgcaacct tgccaaggtt gaggtcgcga gttcgagact
     8701 cgtttcccgc tccaa
//
LOCUS       DAWTCI010000081         8019 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWTCI010000081 DAWTCI010000000
VERSION     DAWTCI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.081"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            332..1009
                     /locus_tag="WM481_001812"
     CDS             332..1009
                     /locus_tag="WM481_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFA9081070.1"
                     /translation="MTFFKPFTVVLTASALALSGCVADPVTGQQSPNKSAMYGLGGAA
                     VCGIVGALTHSGKGARNSALACGAIGAGVGGYMDYQEQRLRQNLAGTQIEIQRQGNQI
                     RLVMPESVTFATGSAALGGSAQYALNTAAQTLVQYPDTTLTINGHTDNTGSDAVNNPL
                     SQHRAQAVAYYLQTRGVAASRLTVYGYGSHMPVASNATVEGRAQNRRVEILINPDQRA
                     VNAARHM"
     gene            complement(1283..2053)
                     /gene="xth"
                     /locus_tag="WM481_001813"
     CDS             complement(1283..2053)
                     /gene="xth"
                     /locus_tag="WM481_001813"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFA9081071.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPSLPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVTSEMAAVLKDVRIDLETRALERPSDHAPVAAEFDL"
     gene            complement(2113..2394)
                     /locus_tag="WM481_001814"
     CDS             complement(2113..2394)
                     /locus_tag="WM481_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9081072.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSCHLNRLRAGGLVDFTRYHRIIEYRLVSEDAATILRTIRDLENKRAA"
     gene            2647..2898
                     /locus_tag="WM481_001815"
     CDS             2647..2898
                     /locus_tag="WM481_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081073.1"
                     /translation="MDIFRESRPFCATGCPTPTSGSAFHHRRRRYGQDRIGRAFLTMH
                     RILDKENQIHTDMALALPAVVKDAVAIWADMDALDRCSG"
     gene            2873..3067
                     /locus_tag="WM481_001816"
     CDS             2873..3067
                     /locus_tag="WM481_001816"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081074.1"
                     /translation="MHWIGVPADRSVTAVSFGYRSSNGSNAEMPKSRLPEMLFFLKHH
                     DLHEKCRLKNISDGIPSLRQ"
     gene            complement(3129..4010)
                     /gene="nadC"
                     /locus_tag="WM481_001817"
     CDS             complement(3129..4010)
                     /gene="nadC"
                     /locus_tag="WM481_001817"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFA9081075.1"
                     /translation="MPSENPLFALPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPCVRFQAEIQDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     ANHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(4086..5024)
                     /locus_tag="WM481_001818"
     CDS             complement(4086..5024)
                     /locus_tag="WM481_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081076.1"
                     /translation="MDAYPEAEAPLQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQKRLKHIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQTMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPGFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDNVLSDRLISDIGLPLGSR"
     gene            5226..6338
                     /gene="nadA"
                     /locus_tag="WM481_001819"
     CDS             5226..6338
                     /gene="nadA"
                     /locus_tag="WM481_001819"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFA9081077.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDHETAGRL
                     KDEIKALLKRKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVAVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKHALTGGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6390..7898
                     /gene="nadB"
                     /locus_tag="WM481_001820"
     CDS             6390..7898
                     /gene="nadB"
                     /locus_tag="WM481_001820"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFA9081078.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIGKHVADTLEAGAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NHNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIIQIRRRPNIRVYERQMALDVQTES
                     GAACGLTVLDCRTQETYRIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     IENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQSGERFMPHYDRRAELAPR
                     DIVARAIAAETAKQTQDFVSLDISRQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPSGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQG
                     IADGQAFQIEPFQRPSESPSAETDIFSNDLQNTFSRPVLQTFNQRHLGILRNDTDLHR
                     AIDQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
BASE COUNT         1744 a         2358 c         2258 g         1659 t
ORIGIN      
        1 tcctaaaccc accatccgcg cctgccgcac gtacacgtcg acaaaccgaa aatatggctg
       61 ctcggcggca tcgtcggcgc gtggggctgc cataaatcgg acaccatttc tccctgcccg
      121 tttcggcatt attgccgata ccgggtgcag gctcggtcgg ctacgatgtg aaggtacgtt
      181 tttttgcgga tgtggctgcg gtttcggcgc aaatcacgat aaacccgcgt tgtttcggga
      241 aaaatcgggt aatttcaggt taattgcact ttttcagacg gcatgattgc ttatagttgt
      301 acggtttgac atatgcacgg aaggaaacgc catgactttc ttcaaaccct ttaccgtcgt
      361 gctgaccgca tccgcactcg cgctttccgg ctgcgttgcc gaccccgtaa ccggacagca
      421 gtccccaaac aaatccgcca tgtacggttt gggtggcgcg gcagtgtgcg gcatcgtcgg
      481 cgcactgacc cacagcggca aaggcgcacg caattccgcg cttgcctgcg gcgcaatcgg
      541 cgcaggcgtg ggcggctata tggactacca agagcagcgt ttgcgccaaa accttgccgg
      601 cacgcaaatc gaaatccaac gccaaggcaa ccaaatcagg ctggtgatgc ccgaaagcgt
      661 taccttcgcc accggcagcg cggcgttggg cggcagtgcg caatacgccc tgaacactgc
      721 cgcacagacg ctggtgcagt atcccgacac gacgctgacc atcaacgggc acaccgacaa
      781 cacaggttcc gatgcagtca acaatccgct ttcgcaacac cgcgcccaag cggttgccta
      841 ctatctgcag acgcgcggcg tggcggcttc gcgcctgacg gtttacggct acggttcgca
      901 tatgccggtc gcgtccaacg ctacggttga aggccgcgcg caaaaccgcc gcgtcgaaat
      961 cctcatcaac cccgaccaac gcgccgtcaa cgccgcacgg cacatgtaac gcgccctgca
     1021 acgccaatgc cgtctgaatg tttttcagac ggcatttccg tattcgcaag cacagccgta
     1081 ccccgactgt aatgctatgg ataacgtctc gccgtttgcc gggaaaccgc agattgcagc
     1141 cggtggcgtg caagtgggcc caaccttgca gacgttaccg ccataccgcg atacccctgt
     1201 aacctcatcg tttaaacaga tattcaatca aaatgccgtc tgaaaccctg cgaagtttca
     1261 gacggcattt gcctttcccg aattacaaat caaattctgc cgccacgggc gcgtggtcgc
     1321 tcggacgctc cagcgcgcgc gtctccaaat cgatgcggac atccttcaaa acggccgcca
     1381 tttcggatgt aaccaatata tggtcgatac gcaggcccag tttgcgttgg aacatcgcgc
     1441 cgcgatagtc gaaccaggta tagaacgcgc cttcggggtg gacttggcgc aggctgtcgg
     1501 tcaatcccaa atccagcaga ttttgaaacc actgccgttc gacggacgaa cagtggattt
     1561 tttcatacca tttttcaggg tcgtaacagt ccgcatcggc aggcgcaata ttgaaatcgc
     1621 ccagcagtac cagtttgccg tggcgggtca tttcgtcgcg gacaaactcc gtcagtgcgg
     1681 caaaccactg ttccttatat ttgaatttgg ggctgtccaa agcctcgccg ttgacgcaat
     1741 agacattgat gacgcgcacg ccgcctacgg tcgccgcaat cacgcggcgt tgcgggtcat
     1801 ccggcaggga aggcaaaccg aaatgcacgt cctgcggcac gctgcggctg acgattgcca
     1861 caccgttgta ggttttctgc ccgctccaaa cacagtgcca gcccatcatc tgcaaggcgg
     1921 cggccggaaa tttgtcctga tcgagtttga gttcctgcaa aaccaaaata tcgggcggat
     1981 tgtcggcaag caggttttgc acctgcggca gccgcacatt gagcgaattg acgttccaag
     2041 tggtgatttt cataacattt ccgaaaaatg ccgtctgaaa cgttcagacg gcaaaagaaa
     2101 aggattctaa cactatgccg cgcgtttgtt ttccaaatcg cggattgtcc gcagaatcgt
     2161 tgccgcgtct tcggaaacca ggcggtattc gataatgcgg tggtaacgcg taaagtccac
     2221 cagaccgccc gccctcaggc ggttcaaatg acaggaaacc gcggtagccg acagggataa
     2281 ggattttgcc agttcgacga tattgcgttc gctgtccaac agttggatca gtatcgccat
     2341 acgttcggga tgcgccataa gtttgagtat ggtgtggagc ggtattgtgt tcatcggtat
     2401 tcctaaaacc ttttccgccc gccaagcgga ttgacgggcg gcagatatgg atgattttga
     2461 ttatctttat aaattttatt ataaatcaat tgtttatttc tcattgtaca ggattgtatg
     2521 ttccttttcc cactattttt gcaaacaagc gtaaagacag gctttcagac ggcataaaac
     2581 tgccgcccct accctgcctc accctgttgc cgaacggaat catccgtatt ttttaagaaa
     2641 accattatgg atattttccg tgaatcaagg ccgttctgcg cgacaggctg tccaacgcca
     2701 acatccggtt cggcatttca tcatcgccgc cgacgatacg ggcaagaccg catcggtcgt
     2761 gccttcctaa cgatgcaccg catcctcgac aaggaaaacc agatacatac cgatatggct
     2821 ttagcgttgc cggcggtagt gaaagacgca gtggcgatat gggcagatat ggatgcattg
     2881 gataggtgtt ccggctgacc gctccgttac tgccgtatct ttcggctacc gttcatcaaa
     2941 tggttccaat gccgaaatgc ctaaatcgcg cctgcctgaa atgctgtttt ttctaaagca
     3001 tcacgacctt cacgaaaaat gccgtctgaa gaatatttca gacggcattc cctccctgag
     3061 gcaataaccg gattacggtt aagcggttcc ttacctgaca tatcagacag ccgcccgcac
     3121 cctgaaactc acgccacgaa atccaaacct atgtccaacg atcggctgct gtgggtcaga
     3181 tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcaggcg gtcgaagccg
     3241 atgccgcccg atgcttcgca atagacggtg tgtgggtggg cggtttgtgt gtggcagcgg
     3301 tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg ttccgcgccc
     3361 gctgcgatgg cttcgtccag ttgtgccaac gtgtccactt cgatttccac gcaggtcaac
     3421 ggtccgaccg cctgttttgc ctgccgaacg gcttgggcga tgctgccgca ataggcgagg
     3481 tggttgtctt tgatgagcac ggcgtcgtcc aaacccatgc ggtggttcgc gccgccgccc
     3541 gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg gctgcacacg
     3601 atgtctgtac cgtattcggc gacctcggca acggcacgag cggtggcggt ggcgatgccg
     3661 ctcaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagcgc gcgggcgttg
     3721 ccttcgacgg cagccagtgt ctgacctgcg cggacggctt gcccgtcttg gatttcggct
     3781 tggaagcgga cgcacggatc catcgtctga aaggcgaggc gcgccaagtc cataccggcg
     3841 ataacaccgt cttcgcggct gacgaggaaa agtttggcgg ttttgtcggg cgcgatgacg
     3901 gcggcggacg taatgtcgcc gcgcctgccc aagtcttcgc tcaaggcttg ttccaccatg
     3961 gggcgcaaca aggtgtcggg cagggcaaag agggggtttt cagacggcat ggggtttcct
     4021 ttgttaaaaa agattgatgt tgcaccgatg gggcaaaact tcatttgatg ccgtctgaaa
     4081 acgggctaac ggctgcccag cggcagtccg atgtccgaaa tcagcctgtc ggacaggacg
     4141 ttgtcgcgga agcggtaaag ctgcgcggga cggcctttgc tgcccgatac gccggtatcc
     4201 gacggctcga taaggttttg ctgctgaatc tggcggcgga agttttgctt gtgcagcaat
     4261 ctgccgctga tggcttcgac gctgttttgc agttgcagca gtgtaaatcc gggcggcatc
     4321 agttcgaaaa tcacggggcg gtatttgatt ttggcgcgca ggcgggacag ggcggtcgcc
     4381 aacacgcggc ggtggtcgtg gcgcatggtc tgccccgtga gcgcgaagtc gaagtttgcc
     4441 tgcggctcgg cggcctccgc tatcagaccg ctttcataca acatttcata gcgttgcaaa
     4501 acgtattctt ccgaccagtt ttccggttcg accccccaac acaaatgaat atgcttgagc
     4561 cgcttttggc ggacttcctc cgtgtccgcc gagtttgccc aaatgcgcag gcggctgacg
     4621 acggcgtcgc gctgcccgcc gtcggtgcgc aagtcttccc atgggaaata gccgtagcag
     4681 tcctgccatt tcgcgtcggg atgcaggatg ctatcggctg cctcgcgcac cagccccaaa
     4741 tagctgacgt acagcacggg catgccgtgt tcgttgcggc ggtgggtatc gacaaaggtg
     4801 taaagctgtt ccacatagcc cataggctgc gaagtctgct tggcgaccca cagctttacg
     4861 cccgcctgca gggaattgcg caggggggaa agcgggccgt tgggcaggag cgtgccttgg
     4921 gcgacggtca acacccgcag gccgccgtcg gtaacggcaa tcaatacggg aaccagctcg
     4981 acgatgcttt gcagcggagc ttcggcttcg gggtaggcgt ccatgctcgg ctttctgcgg
     5041 gtgggaaagg ctttattgta aaccaaagcg gtttgtcgga acgaagcggg tcggttttgc
     5101 ggtatcgcaa acctgttttc agacgacctt gcatattata ctcaagttga gcataatgta
     5161 aaaacagttt gaaaaacaga cggcaaacct tcatgccgtc tgaaaaccag ctacaaggaa
     5221 acatcatgca aaccgccgcc cgccgctcgt tcgattacga tatgcccctc atccaaacgc
     5281 cgacttccgc ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg cccgaccacg
     5341 agacggcagg tcgtctgaaa gacgaaatca aggccttgct gaagcggaaa aacgcggtct
     5401 tggtggcgca ttattacgtc gatccgctga ttcaggattt ggctttggag acgggcggat
     5461 gcgtgggcga ttcgctggaa atggcgcgct tcggcgcgga acacgaagcc ggtacgctgg
     5521 tagtggcggg cgtgcgtttc atgggcgaga gcgcgaaaat cctctgccct gaaaaaacgg
     5581 tgctgatgcc tgatttggaa gcggaatgtt ctttggattt gggttgtccc gaagaagcgt
     5641 tttcagcgtt ttgcgaccaa caccccgacc gcacggtggc ggtgtacgcc aatacttccg
     5701 cagctgtgaa agcgcgtgcc gactgggtgg taacgtcttc ggtggcgttg gaaatcgtgt
     5761 cgtatctgaa atcgcgcggc gagaaactga tttgggggcc cgaccgccat ctcggcgact
     5821 acatccgccg cgaaacgggc gcggatatgc tgttgtggca gggttcgtgc atcgtccata
     5881 acgaattcaa agggcaggaa ctggcggcgt tgaaggcaga gcatcccgat gctgtggtgc
     5941 tggttcatcc cgaatcgccg caaagcgtca tcgaactggg cgacgtggtc ggctcgacca
     6001 gcaaactact caaagccgcc gtatcgcgcc ctgaaaaaaa attcatcgtg gcaaccgatt
     6061 tgggcatcct gcacgaaatg caaaagcagg cgcccgacaa agaatttatc gccgcgccga
     6121 cggcgggcaa cggcggaagc tgtaaaagct gcgcgttctg cccgtggatg gcgatgaatt
     6181 cgctgggcgg catcaaacac gccctgacag gcggacgcaa cgaaatcctg ttggacagaa
     6241 agctgggcga agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg gcaggactca
     6301 aacgcgggga tgtgttcaac ggcatggggc ccgcctgatt tgccgtccat atcgccgttt
     6361 cagacgacct ctcaaacaag gacaacacca tgcaaaccga ttgcgacgta ttgattgccg
     6421 gaaacgggct ggcggcactg acgctcgccc tgtcgctgcc tgaatcgttc cgcatcgtca
     6481 ttttgtgcaa aaaccggctg gacgacaccg ccagccgtca tgcgcaaggc gggattgcgg
     6541 cggcgtggtc gggagaggac gacatcggaa aacacgtcgc cgatactttg gaagcgggcg
     6601 caggtttgtg cgacgaagcc gccgtccgca ccatcctgtc gcagggcaaa ccggcaatcg
     6661 aatggctgct ggcgcagggc gtggcgttcg accggaatca taacgacctg cacctgacgc
     6721 gcgaaggcgg gcatacctgc cgccgaatcg cccacgtcgc cgactacacg ggcgaagccg
     6781 tcatgcagag cctgattatc caaatacgcc gccgaccgaa catccgcgtt tacgagcggc
     6841 agatggcgtt ggacgttcaa accgaatcag gcgcagcgtg cggactgacc gtcctcgact
     6901 gccgaacgca agaaacctac cgcatccgtg cccgccatac cgtactcgca ggcggcggcc
     6961 tcgggcaaat ttacgccgcc accaccacgc cgcccgaatg cacgggcgac gccatcgcca
     7021 tggcgatacg cgcgggttgc gcaatcgaaa acctcgaatt tatccaattc caccccacag
     7081 gcttggcaag gccgtctgaa aacggccgca ccttcctgat ttccgaagcc gtgcgcggcg
     7141 aaggcggcat cctgaccaat caatcgggcg aacggtttat gccgcattac gaccgccgcg
     7201 ccgaactcgc gccgcgcgac atcgttgccc gcgccatcgc cgccgaaacc gccaaacaaa
     7261 cgcaagactt cgtctcgctc gacatcagcc gccaacccgc agcgttcgtc cgtcggcatt
     7321 tcccgtccat ccatcggcac tgcctgtccc aatgcggctt ggacatcacg cgccaagcca
     7381 tccccgtccg ccccgtgcaa cactacacct gcggcggcat ccaaaccgac cccagcggca
     7441 gaacctccct gccgcagctc tacgccttag gcgaaaccgc ctgtaccgga ctgcacggag
     7501 ccaaccgcct cgccagcaac tccctgctcg aatgcgtcgt taccgccagg cttgccgccc
     7561 aaggcatcgc ggacggacaa gcgttccaaa tcgaaccatt ccaaaggccg tctgaaagcc
     7621 cctccgccga aacagacatc ttttcaaacg acctccaaaa cacattcagc cgccctgtcc
     7681 tgcaaacgtt caaccaacgc catctcggca tcctccgcaa cgataccgac ctgcaccgcg
     7741 ccatcgacca actgcggctt tggaagcaaa accaagccga accgcacacc gcgtccgaat
     7801 acgaaaaccg caacctactc gaatgcagcc tcgccgtcgc ccaagccgca tacaggcgga
     7861 gacagaacat cggtgcgcat tttaatagtg attgttaagg ttggctggtt agaaaaaaat
     7921 cgttcaaata aatttgaata gcatggttaa gagattggca ttcagttttt atggttgcgt
     7981 aagttgggtt gaggcctttg caaaaaagcc cttccttcg
//
LOCUS       DAWTCI010000082         8017 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWTCI010000082 DAWTCI010000000
VERSION     DAWTCI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.082"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(1..345)
                     /locus_tag="WM481_001821"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="WM481_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(345..1646)
                     /gene="nuoF"
                     /locus_tag="WM481_001822"
     CDS             complement(345..1646)
                     /gene="nuoF"
                     /locus_tag="WM481_001822"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFA9081079.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVRRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFAHYIEHGGPMKEHKWGGW"
     gene            complement(2036..2509)
                     /gene="nuoE"
                     /locus_tag="WM481_001823"
     CDS             complement(2036..2509)
                     /gene="nuoE"
                     /locus_tag="WM481_001823"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFA9081080.1"
                     /translation="MLSAKSLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(2509..3765)
                     /gene="nuoD"
                     /locus_tag="WM481_001824"
     CDS             complement(2509..3765)
                     /gene="nuoD"
                     /locus_tag="WM481_001824"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFA9081081.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPGFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCADWLRVNPGPVITANHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(3755..4348)
                     /locus_tag="WM481_001825"
     CDS             complement(3755..4348)
                     /locus_tag="WM481_001825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFA9081082.1"
                     /translation="MASIQNLYETVVGVLGDQAGKVISALGEITVECLPEHYISVMTA
                     LHDHEDLHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVADIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(4361..4843)
                     /locus_tag="WM481_001826"
     CDS             complement(4361..4843)
                     /locus_tag="WM481_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HFA9081083.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(4834..5190)
                     /locus_tag="WM481_001827"
     CDS             complement(4834..5190)
                     /locus_tag="WM481_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFA9081084.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            complement(5683..7134)
                     /locus_tag="WM481_001828"
     CDS             complement(5683..7134)
                     /locus_tag="WM481_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HFA9081085.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTPFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIAGRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDIFNSVNGIERAYLPPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIRMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(7157..7777)
                     /locus_tag="WM481_001829"
     CDS             complement(7157..7777)
                     /locus_tag="WM481_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFA9081086.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSKGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACLNLLIAASVWLRYRKDGYGEH"
BASE COUNT         2099 a         2008 c         2013 g         1897 t
ORIGIN      
        1 aacttttcac accaattaat tatatttaca tcgccatgca atatcctact gcatcgcaac
       61 taccaccccg tgtccttgac gtgccaaaat aaaatcacaa ttatgcgaat aaagtttgta
      121 taaatttctc ctaacctcgt attcattatc accagatgaa taaaaaccaa tcctctgacc
      181 ttcatgatca aatactgcaa caaaatctaa gccgtttcca tatctttcaa aaaagactag
      241 aaatttagaa aatttccgac aaatctgatt aaaaagcgta ttgagtgtcg tactcttacc
      301 tttgttggct gcaccgtata gaataaaaat tttagcttcc accattacca ccctccccac
      361 ttatgctctt tcatcggccc gccgtgttcg atgtaatgcg caaactcatc acggaaatgc
      421 ttggtaaagc tgcggacagg gaagacggcg gcatcggcaa gggcgcagat ggtgcggcct
      481 gccatttggt tgccgacaga atccagcaaa tccaagtctt ccatgcggcc tttgccttct
      541 acgatgcggc ggacgatgcg gtaaagccag ccggtacctt cgcggcaggg ggtgcattgg
      601 ccgcaggatt cgtcgtagta gaaatagctt aagcgttcga gggctttgac catgcacacg
      661 tcttcgtcca tcacgataat cgcgccggaa ccgagcatcg agccggcttt ggaaatcgag
      721 tcatagtcca tattggtctg catcataatg tcggcaggca atacgggggc ggacgaaccg
      781 ccgggaataa cggctttgag ttttttaccg ccgcgcatac cgcccgccat tttcaagact
      841 tcggcaaacg gcgtgcccaa cggcacttca tagttgcccg gacgctcgac atggccggaa
      901 atacaaaata atttggtacc gcctgcattc ggaatacctt tatcggcaaa cgcctgtccg
      961 ccgtcacgga taatgaatgg aacggaggag aacgtttcgg tattgttgat ggtggtcggt
     1021 ttgccgtaca ggccgaacga ggcggggaac ggcggtttga agcgcggctg gccttttttg
     1081 ccttccaacg attcaagcaa tgcggtttct tcgccgcaaa tatatgcgcc gtagccgtgg
     1141 tgggcgaaga gttcgaattc aaaatccgaa cccaaaatat ttttacccaa aaagcctgcg
     1201 gcacgcgcct gctccaaagc ggcttcaaag cgttgataac cttcgaaaat ttcgccgtgg
     1261 atatagttgt agccggcttt cgcgcccatc gcgtaaccgg caatgatcat gccttcaatc
     1321 agggcatgag gattgaacat gatgatgtcg cggtctttaa atgtacccgg ctcgccttcg
     1381 tcggtgttgc aaaccacata tttttcgccc gggaaagaac ggggcataaa gctccatttc
     1441 aaaccggtcg ggaagcccgc accgccgcgc ccgcgcaagc cggaggtttt gacttcgtca
     1501 atcacatcgg tttgcgagat gttttcggac aggattttac gcagggcggt atagccgccg
     1561 cgtttgacgt attcgtccaa tgtccagcaa tcgggattgg cggtatccac ttggtcaaaa
     1621 atcacgcctg attggtaaat agccattttt ggtgtgcctg tttgttttcg tatcggttgc
     1681 ggccgctgtt tcagacgacc ttaaaatgtc tttgtgtatc ggcttgtaac gtcttctgaa
     1741 ataaaatcta gtttatcaaa tcgatatttt cagacgacct tcaatcgttt ttgttggatc
     1801 tcgaccactt gcttgtctgc cgtcattccc gcgcaggcgg gaatccatcc tcaatggtaa
     1861 gcaatgtctt attaaattca gaaaccgaat cttaccggtg gattcccgcc tgcgcgggaa
     1921 tgacggcatt tcagtatttc agtagggcgg attcttaaat ccgacatttt gcctttttac
     1981 ccactctgtc ggatacaagt atccgaccta cgtttaaatc gtcgtttcag acgacctact
     2041 ccaactccgc cagtttcttc tcaatcgcct cttcggtcat aaagctgcac atgctgtggt
     2101 tgttgaccag cataacgggg gcatcgccgc acgcgcccat gcattcgcct tcgacaaggg
     2161 taaatttgcc gtcgggcgtg gtttcgccgt agccgatacc gagtttttgt ttgaggtatt
     2221 cgccggtcgc cataccgccg cgcagagcgc agggcaggtt ggtacaaacg gtcagtttgt
     2281 atttgccgac aggctcaagg tcgtacatat tgtagaaagt ggcgacttca tatgcctgcg
     2341 ccggcgtgat gccgatgtag tcggcaacaa aagcgatggt ttcgggggcg agccagcctt
     2401 tttcggtttg ggcgatacgc aacgcgccca tgatggcgga acggcgttgg tcggcgggat
     2461 atttcgccaa ctcaatgtcg atttgtttta aggattttgc ggataacatt atcggtcaac
     2521 ctccccgaat acgatgtcct gcgtaccgat gatggcaacg acgtcggcga gcatatggcc
     2581 ttttgccatt tcgtccatgc cttgcaggtg ggcgaagccg ggtgcgcgga ttttcaggcg
     2641 gtagggtttg tttgcgccgt ctgaaatgat gtaaacgccg aactcgcctt tcgggtgttc
     2701 gacagcggtg taggtctcgc cctcgggaac gtgcataccc tcggtaaaga gtttgaaatg
     2761 gtgaatcagg tcttccatac ctgttttcat ttcggtacgt ttgggcggag cgaatttgtg
     2821 gtttgcggta atgaccgggc cgggattgac gcgcaaccag tcggcgcatt gtttgatgat
     2881 gcgtacggat tggcgcattt cttccatacg gcagaggtaa cggtcgtagc agtcgccgtt
     2941 gacgccgacg gggatgtcga aatccatttt gtcgtacact tcgtagggct gtgtcttacg
     3001 cacgtcccat tccacgccgg aaccgcgaag catcacaccg gtaaagcctt tttgcatggc
     3061 gcgctcggga gagacaacgc cgatgccgac ggtacgctgt ttccagatgc ggttgtcggt
     3121 caggagggtt tcgagtgtgt cgatattttt ggggaagcgt tcgcagaagg catcgataaa
     3181 gtcaagcatc gtgccttcgc gggattcgtt gagctgcttc aatactttgg cattgcggaa
     3241 tttgctgctc tcgtatttgg gcataaagcc gggtaggtcg cggtaaacgc cgccgggacg
     3301 gaagtaggcg gcgtgcatac gtgcgccgga cacggcttcg tacaagtcca tcagctcttc
     3361 gcggtcgcgg aaggcgtaga ggatggcggt catcgcgccg atgtcgaagg cgtgcgaacc
     3421 gatgcccatc aagtgattga ggatgcgcgt tacttcggcg aacatcacgc ggatgtattg
     3481 ggcgcggata ggcacatcga taccggcaag tttttctacc gccaagcaat acgcctgctc
     3541 gttgaccatc atggaaacgt agtccaaacg gtccatatag ggcagggctt gcagataggt
     3601 tttggtttcc gccagttttt cggtgccccg gtgcaggagg ccgatatgcg gatcggcacg
     3661 gacgatttgt tcgccgtcca gctccaaaat catacgcaat acgccatgcg ccgcagggtg
     3721 ctgcgggccg aagttgatgg tgtagtttct taatttattg gccaccgtag ttctcctcac
     3781 ggacgatacg cggtgtgatt tcgcgcggct caatggtaac aggttggtaa atcacgcgtt
     3841 tttgctcttc gtcataacgc atttccacat agccggaaat cgggaagtct ttgcggaacg
     3901 gatgtccgac aaagccgtaa tcggtcagga tgcggcgcaa gtcgggatgg ttgttgaaca
     3961 tgatgccgta caaatcgaag gcttcgcgct cgtaccaatc cgcgctgttg taaatatcgg
     4021 ctacggattc gactacaggg aagtcgtcgt ctgaaaccca gacgcgtacg cggatgcgtt
     4081 gattgttttt aacggaaagc aactgactga caacggcaaa gcgtttgccc tgccatgctt
     4141 cgtttttgta agtgctgtaa tcgacgccgc acaagtcaac cagaagctcg aaatgcaaat
     4201 cttcatggtc gtgcaatgcg gtcatgactg aaatatagtg ctcgggcaga cactcgacgg
     4261 taatctcgcc caaagctgaa atgactttgc ctgcctgatc gccaagcacg ccgacgacgg
     4321 tctcgtataa gttttgaatg cttgccatat cgtcctctcc ttactcgtca cgcgcgatgg
     4381 tggaagtgcg cttgattttt tgttggagct gaatcaggcc gtaaatcaga gcttccgcag
     4441 tcggcggaca acccggcaca taaacgtcca ccggcacgac acggtcggca ccgcgcacaa
     4501 cggaataaga atagtgataa tagccgccgc cgttggcaca cgaacccata gacaataccc
     4561 agcgcggctc ggcgagctgg tcgtacacgc gacgcagggc tggcgccatt ttattggtca
     4621 gcgtacctgc cacaatcatc aggtcggcct gacgtgggga cggacggaaa atgataccga
     4681 aacggtcaag gtcataacgc gccatacccg catgcatcat ttccacggcg cagcaggcca
     4741 atccgaaagt aaccggccac aatgaaccgg tacgcatata gttcaacacc gtatccgcgc
     4801 tggtggtgat gaaacctttt ttcaaaacgc cttctattcc cattccagcg cacctttttt
     4861 ccattcgtaa acaaagccta ccgtcaggac gacgataaac accagcatag accaaaagcc
     4921 gtatgcgccc aaatctttga acacgactgc ccacggcagc ataaacgcaa cctccaaatc
     4981 gaagaggatg aacaggatgg cgacgaggta atagcgcacg tcgaacttca tccttgcgtt
     5041 ttcaaaggct tcaaaaccgc attcgtaagg cgcgtctttt tcggcatagt ggcgtttcgg
     5101 gcccaaaatc gtgccgagca ggataaacag tacgccggcc gcgaggccga cgaggataaa
     5161 gacaaagacg ggaaaataag cggacaacat ggttacaccc aaatccgtta acaaaatttc
     5221 tacaataatt tcgtatttta gcgaatttca aaaaccatta aaaggtaaat atcggcaaaa
     5281 cgcccaaaaa aacccaataa atacaatcat gttatgataa cgattcttat ttgattttat
     5341 aaggtcatat aaatttttac accgtaattc gtggtatagc ggattaacaa aaaccggtac
     5401 ggcgttgccc cgccttagct caaagggaac ggttccctaa ggtgcccaag caccaagcga
     5461 atcggttccg tactatccgt actgtctgcg gcccgccgcc ttgtcctgat ttttgttaat
     5521 ccgctatata taggaataag aattcgggac aaaatgttta aaccattttg tcccgactgc
     5581 tgtgatgcgg tttttttgaa ctagcaggac gttacctaaa acctgcgttg cctaaaacct
     5641 gcgttgccta aaacctgcgt tgcctgaaac cctttaagac ggttaaatcc ctctgccgta
     5701 tttgtattct acaatcatat tatcgtcagt aatgacttcc gccccagccg aaggttccgt
     5761 catccgaatc agcatacgag agacaacctt ttgtgctgca gcatccacgg tgctgctgtc
     5821 aaatacgtgc ctgccgcttt ccggccaaat caaccgggaa aggcgttgct tgagcagttc
     5881 tttattaggg aaaactaccg gggttgccga gccgactacc atatgcccgt agcggtatgc
     5941 atagggaata ctgtgtacgg cggtagcaaa agcatgcggg ctgtgcgtgg tattaaacat
     6001 tacaatacca tccggggtaa ggtggctttg cacctgtttt aaaaattccg cactcaacag
     6061 gttagtggaa taggcacgcc agtaccaagt cgaattcatc aaaatcaggt cgaatttttc
     6121 atcaggatgg cgacgcagcc atttcctacc gtcatccaat acaatttcaa cacgtttgtc
     6181 ctgcaaaagc ggtgcgattt gcggctcgtc cgcgataagg ctacggtatg ccggattgat
     6241 ttccgcaacg atcatcgact gcatttccgg aatggcagac aagacgcgcg cccacgaacc
     6301 tgtactcaat ccaacgacga aaatgcggcg tatgccggac ttcagggagg gtggcagata
     6361 ggcacgttcg atgccgttga cactattgaa tatatcggta ttgtatgcgc cgtcgtatac
     6421 attcgcccca taaacaacct tatcaccatc tctatggtaa accgcaacaa tgccgtgttt
     6481 gttttcaatc aacctatccg gacggccagc aatattttga aagacggaat ccggcagtag
     6541 gaacatgagg atgccgaaca ttagggaaac tgctaccgac actgcattca gtcggagact
     6601 tttttggaac ggtgtacaaa acaaagggac agcagcagaa atcaaacaga tgagcaggta
     6661 aatctgttgg gtggacaaga aatcaagtat cacaaagccg ataaggaccg gacccaatgc
     6721 actgccggca acgttggcga aataaacatt ggaaacctgt cgtccggatt tgttgccatc
     6781 cgtacccaca tggtgtacaa gtgggaaaat caaccccctg acgacggcag acagggtaat
     6841 gaagataccg gcgtggtgga cgaagccgga aaaacccgtc aacaaccacg cagcacccaa
     6901 aatcaaaaaa tcggcaatac ccgcccacaa gaagcactgc ccgataaagg gaatatcaac
     6961 aaagcggctg cggcaaatcc gtttgccaaa atacgcgccg acggcgatac cggtcagaaa
     7021 acaggcaaga gtaaatgaaa atgcctgagg cacggactgt gctgcgaacg aaaacatcct
     7081 tacccacaag acttctatac ccaagctcaa taatccgcta aggaaagaaa gcatataaat
     7141 caaactagta ttcggtttag tgttcaccat atccatcctt tctgtaacgc agccatactg
     7201 aagcagcaat cagaaggtta aggcaggctg tcagcgcaat ggtttgggag agggtaaaaa
     7261 agacgtagaa aaattcggcg gcggcaagcg atccgagtgc cgcacccaaa gtgttgaaaa
     7321 aatataaggt accgatagac tcgccaacat tatgtatttt ccggttaaaa aaacaggtca
     7381 gcaagggcaa ggtcgcgccc atcataaagg taggaagcag caataagagg aaattggcag
     7441 cagcgatgat gggcaaatca gcctcaacta aaagatgccc caagccggaa atcagaccct
     7501 tgcttaccaa accgaacaga ccgatggata cttcagcgat gcaaaacagg gggatgatac
     7561 ttgaaggaaa acggtcagca atgcgtccgc cgaaatacgc acctacaccc aagccgacca
     7621 taaatacaga aataatgaca gtaatcgaac tcaaatcgat acctatgtgg ctgaatagaa
     7681 gcctctgcca gctgacctgg taaatcaggg cgcagaagcc agaggcgaaa aacaccagcg
     7741 acaatccttt cagggtttta cttgcggtcg aattcatatt ttctattttc aaataggcgg
     7801 aactataaat gaaatgtatt aatgtgtaaa catttgtatt agccgtagga aatcaccgca
     7861 ttgctcaaac tgcaggccgg gaaaataaac tcgtaagccc gcaaacggcg tatcactgtt
     7921 caaactgctg tgcggcttca tgacgttata aaaattaagg ggccgtcctg gacaactagg
     7981 ataaactcga ttttactaat tgttttaaaa tggaaca
//
LOCUS       DAWTCI010000083         8004 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWTCI010000083 DAWTCI010000000
VERSION     DAWTCI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.083"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            81..3509
                     /locus_tag="WM481_001830"
     CDS             81..3509
                     /locus_tag="WM481_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9081087.1"
                     /translation="MMSGFSPKPKTIILSLAGAFGALAFADTPNNTEQQKELNTIVVH
                     GKRSADQKGADDVYYKNVSNAYVGKEYLERYRVQSAGDVLKGLNGVYNMNTRTAGGAI
                     TPNIRGITGKGRIPVTIDGTEQTIDVWMNNYGVGDRNYLDPALFRSIAVEKSPALTRG
                     VKSGVGGAMSIRTIEAGDIVPEGQKFGFQLKTEFANNRTRPANNLNQWLGWEDYRTLP
                     LGATADGAGGGSDPITGQQSPQALVVDNFTPPQHKSGRDNWRFGGDRSYMAAAAFKTE
                     LSDGLAAYSYRNKGNYFAGRKGAEGYLNNPVYDLQKCYDEGGSDFNCKNSATFIPNMA
                     RIYHPGVEVLNSNTETKTLLLKNNWHLPGSHRLGWQYMRTDVRFGEINPFHTTYVMNM
                     EEHNETNRPKELSPQAQSIDSTIRTDTYKLGWAWKPENSRWVDLQANLWRIKTNSTRH
                     QSGGMDLSSARPDPFYDVWYWCTQRGRIPPEHVDNYSSCNDLMNDFGVNGLTKEQVLA
                     MTPNDNGQFRVISGAEQKTRVSRTGFDISNRFRLRDRLSMTLAADYQKEKLAEEVEIV
                     NSQDLFNLAGMVTGLTKLAGPRGGERREWGTNLVFDWQAADRLKISAGIRYHNFRGFD
                     TALAEGRARRDPRYQAGDGTQHYADGAYLPYFELAGDQERRDWNVVAEQLRQAYQSGD
                     AAAIAAAVQAEQAHGARYHLLKYYDGSYRTDGYVYIGNDGRAYFDGRFGGVNEAQNQP
                     LYRVRPVYVPFVNGKLDSGALPQHFHDFVSNYQEKVTNPQGLHGTHYRYWAGVGNPID
                     CIRSDAASCLAHNLSGKVTNFQVGYPEYGDDTGLGLKIIGRHYTEEQYWAQPKPIRAH
                     AWAPTIAVSYDLTDNSRLFVRYAQMSRFPSVYEVGSFYNDVAYAGMPKAPNFRFKPER
                     SRSWEIGYSFNFAPYWSRLRAGDMRLTYYRNRIQNVIETTDYFRTVQYDRKDTAGLEW
                     QSRIDTGRFFAALGATYRLKQQMCDRDTAFDYDPYGAKGVPICIEGGYGSTRGYQALQ
                     PKYSINLDAGVRLLGEKLELGLRGIYHSRVNTKQYDQLLQKDLGIIFDTTGKPHHWRS
                     SLTWDVYGRYRLHKNLNVNLGITNLTNRYYLDPMSNVAAPGPGRTVTFGLTAKF"
     gene            3724..4707
                     /locus_tag="WM481_001831"
     CDS             3724..4707
                     /locus_tag="WM481_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081088.1"
                     /translation="MSIRNKTLLAVLLCGVFGVAAAAPAGGSSQRKSQDPNSDPTTVR
                     PGTSDKQPFGAGKAGLAFSINNGGSWSKSINLDDGPVKYIRAKYQAPINGNDPYANVD
                     GTRNLQYLRLGDVKPVIGWFYNPTLGQVWYENHHNNTEVYSVRQIANPAIPAAPKFGG
                     LVIAKVPGMGGSNGNVFFGEWAPRAGNPQQISTDLNMADGKRTVWYVGDNPTGNTTGV
                     RTATYNVLGINKHTPGQNDFYTGQVTATFGSGITGTMAGRISRSSDHVDFDHAKIDNR
                     NGTFADPSQGMTGRFYGNQAAAMAGYTTRGTGNVGDDVAFGGSATDIETRK"
     gene            4978..6465
                     /locus_tag="WM481_001832"
     CDS             4978..6465
                     /locus_tag="WM481_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HFA9081089.1"
                     /translation="MNKPLSLALFCLLLPITAHADNPADERRLLDEGSRQTQQYRESG
                     WLDTEQARGEVEENDGYISIGGEIYQVGDTAEELESAIYHALNARQWHKVRQFAARYA
                     KLPRHKPALIHLADALQKRDEGDFRAAGNSFQTALEAEPDNPRLLLEAGRFYAEDNQN
                     KESAAAFEKVLKTDIPAETRPIVENYLSELGKRRRWHGQISLGYGYNSNVNQGNGINQ
                     CVWEIAGMCLMERTLPAPTDSTFSSYSATAEKTVPLKGHHGVQVRGVLYGNRYTEKDK
                     DSAAMPDYGYRNGSLYAGYAYADARSSFSLLPYFEYDFRNRHTHYRAWGADADWSRTL
                     SPHWRINSHAGAKKTGYGGQSKTYFADFKQYELGAGAEFSITPKSGLLVNFDAARKAY
                     PEKSSSSKEYTARLGAYRLFSGGTYLNAVLLYRRSLYDAASFVSDNKRRSDKQYIMMA
                     AAGFPQWNIKGVYPELRFRRTIAHSNAVYYRYRQNEWLLGFKYRF"
     gene            complement(6533..7930)
                     /gene="aspA"
                     /locus_tag="WM481_001833"
     CDS             complement(6533..7930)
                     /gene="aspA"
                     /locus_tag="WM481_001833"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFA9081090.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEAFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAESGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHRNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         1911 a         2317 c         2152 g         1624 t
ORIGIN      
        1 ataataatta aaaatttcaa ataattctta ttatgagtgt atcttaataa aattcatttt
       61 atattcttaa taaggaatac atgatgtccg gattctcccc caaacctaag accattatcc
      121 taagccttgc aggtgcattt ggcgcattgg cttttgcaga caccccgaac aataccgaac
      181 agcagaaaga actgaatacc atagtcgtcc atggcaaacg cagtgccgac caaaaaggcg
      241 cggacgacgt gtattacaaa aacgtctcca acgcctacgt cggcaaagaa tacctcgaac
      301 gctaccgcgt ccaatccgcc ggcgacgtac tcaaaggcct aaacggcgtg tacaacatga
      361 acacccgcac cgccggcggc gccatcacgc ccaacatacg cggtatcacc ggcaagggcc
      421 gcatcccggt taccatagac ggcaccgaac aaaccatcga cgtatggatg aacaactacg
      481 gcgtgggcga ccgcaactac ctcgaccccg ccctgttccg cagcatcgcg gtggaaaaaa
      541 gccccgccct cacgcgcggc gtgaaatcgg gcgtcggcgg cgcgatgtcc atccgcacca
      601 tcgaagccgg cgacatcgtg cccgaagggc agaaattcgg cttccagctg aaaaccgagt
      661 ttgccaacaa cagaacccgc ccggccaaca acctcaacca atggctgggc tgggaagact
      721 accgcaccct gccgctcggc gccaccgccg acggcgcggg cggcggttct gacccgatca
      781 ccggacagca gagcccgcag gcgttggtgg tagacaactt taccccgccg cagcacaaaa
      841 gcggccgcga caactggcgc ttcggcggcg accgcagtta tatggccgcc gccgccttca
      901 aaaccgaact ttcagacggc cttgccgcct acagctaccg caacaaaggc aactacttcg
      961 ccggtcgcaa gggtgcggaa ggctacctga ataatccggt atacgacctg caaaaatgct
     1021 acgacgaagg cggcagcgat ttcaactgca aaaattccgc caccttcatc cccaatatgg
     1081 cgcgcatcta ccaccccggg gtggaagtgc tcaacagcaa caccgaaacc aaaaccctgc
     1141 tgctgaaaaa caactggcac ctgcccggca gccatcggct cggctggcaa tatatgcgca
     1201 ccgacgtgcg cttcggagag atcaacccct tccacaccac ctatgtgatg aatatggaag
     1261 agcacaacga gaccaaccgc cccaaagaac tgtcgccaca ggcgcagagc atcgattcca
     1321 ccatccgcac cgacacctac aaactcggct gggcctggaa gccggaaaac agccgctggg
     1381 tcgatctgca ggccaacctg tggcgcatca aaaccaacag cacccgccac cagagtggcg
     1441 gcatggattt gtcttccgcc cgccccgatc ccttctacga cgtttggtat tggtgtaccc
     1501 aacgcggccg gataccgccc gagcacgtcg acaactacag cagctgcaac gacctgatga
     1561 atgatttcgg cgtcaacggc ctgaccaaag aacaggtgtt ggccatgacc ccgaacgaca
     1621 acggccaatt ccgcgtgatt tccggcgccg agcagaaaac ccgcgtcagc cgcaccggtt
     1681 tcgatatcag caaccgcttc cgcctcagag accgcctgag catgaccttg gccgccgatt
     1741 atcagaaaga gaaactggcg gaggaagtgg aaatcgtcaa ttcgcaagac ctgttcaacc
     1801 tggccggcat ggtaaccggc ctgaccaagc tggccgggcc gcgcggcggc gagcggcgcg
     1861 aatggggcac caatttggtg ttcgactggc aggccgccga ccgcctgaaa atctccgccg
     1921 gcatccgcta ccacaacttc cggggcttcg ataccgccct ggccgaaggg cgtgcccgcc
     1981 gcgacccgcg ctatcaggca ggcgatggaa cccagcatta tgccgatggt gcctatctgc
     2041 cgtatttcga actggcaggc gatcaggagc ggcgtgactg gaatgtggtg gccgaacagc
     2101 ttcggcaggc gtatcagtca ggcgatgctg ccgccattgc cgcggcagta caggcagaac
     2161 aggcgcacgg cgcccgttac catttgctga aatactatga cggctcgtat cgtaccgatg
     2221 gttatgtgta tatcggcaac gatggccgtg cctattttga cggacggttc ggcggggtta
     2281 acgaggcaca gaaccaaccg ctctatcggg tgcgccccgt ttatgtgccg tttgtcaacg
     2341 gcaagctgga cagcggcgca ttgccccagc attttcatga tttcgtcagc aattatcagg
     2401 aaaaagtgac caatccgcag gggctgcacg ggacgcatta tcgatattgg gcaggcgtgg
     2461 ggaatcctat cgactgtata agaagtgatg cggcctcttg ccttgctcat aacctatcgg
     2521 gaaaagtaac caattttcaa gtcggctatc ccgagtacgg tgatgatacc ggtctcggtc
     2581 tcaaaatcat cggtcggcac tataccgagg aacagtattg ggcgcagccc aaaccgatcc
     2641 gtgcccatgc ctgggcgccg accatcgccg tcagctacga tctgaccgac aacagccgcc
     2701 tgttcgtacg ctatgcgcag atgagccgct tccccagcgt gtatgaggtg ggcagttttt
     2761 ataacgacgt ggcctatgcc ggtatgccga aggccccaaa tttccgcttc aagccggaac
     2821 gcagccgcag ctgggaaatc ggctatagct tcaattttgc cccgtattgg agccggctgc
     2881 gtgccggcga tatgcgcctg acctactacc gcaaccgtat ccaaaatgtg atcgaaacca
     2941 cagattattt ccgcaccgtg caatacgacc gcaaggacac cgccgggctg gaatggcaga
     3001 gccgcatcga caccggccgc ttctttgccg cgctcggtgc cacctaccgc ctgaaacagc
     3061 agatgtgtga ccgcgatacc gctttcgatt acgaccctta tggtgccaaa ggcgtgccga
     3121 tctgcatcga aggcggctac ggcagtacgc gcggctatca ggccttgcag ccgaaatatt
     3181 caataaacct ggatgccggg gtgcgcctgt tgggcgagaa actggagctc ggcctgcgcg
     3241 gcatctacca cagccgggtc aacaccaagc aatacgacca actgctgcaa aaggatttgg
     3301 gcatcatctt cgataccacc ggcaagcccc accattggcg gtcatcgctg acttgggatg
     3361 tttatggccg ctaccggctg cacaaaaacc tcaacgtcaa cctcggcatc accaacctga
     3421 ccaaccgcta ctatctcgac ccgatgtcca acgttgccgc ccccgggccg ggccggacgg
     3481 ttaccttcgg gctgactgcc aagttctagc cgcagggcgg agtggggttt tcaggtagcc
     3541 tgcgggtatg gcgggaaggc tacctgaaaa cggattggat ataacaacaa acaattgaac
     3601 actccggacg cacaaaccca accggccccg cttatccgag gtaatggttt ttaccgtttt
     3661 ttccgccggt tcggcggtaa taaaacggtg gcttttgttt aactcttaat taaggaaacg
     3721 attatgtcta tacgtaataa aaccctgttg gccgtcttgc tgtgcggcgt ttttggtgtg
     3781 gctgcggctg ctccggctgg tggttccagc caaaggaaaa gccaagatcc aaatagtgac
     3841 ccgacgacag ttcgccccgg cacctctgac aagcaacctt tcggtgccgg taaggccggt
     3901 ttggcattca gtatcaataa cggtggaagt tggtcgaaat ccatcaatct ggatgatggt
     3961 ccggtcaaat atatccgtgc gaaatatcag gcaccgatta acggcaacga tccctatgct
     4021 aatgttgatg gtacgcgcaa tctgcaatat ctgcgcttag gcgatgtgaa accggttatc
     4081 ggctggttct acaacccaac gttgggtcag gtgtggtatg aaaaccacca caacaacact
     4141 gaagtgtaca gtgtgcgcca gatagccaac ccggcaatcc ccgccgcacc caagttcggc
     4201 ggcctggtaa tcgccaaagt gccgggcatg ggcggcagca acggcaacgt cttcttcggc
     4261 gaatgggcgc cgcgtgccgg taacccgcag caaatcagca ccgacctgaa tatggcggat
     4321 ggtaaacgca ccgtttggta tgtgggcgat aatcccaccg gcaacaccac cggtgtgaga
     4381 accgccacct acaatgtgct gggtatcaat aagcacaccc cggggcagaa cgatttctat
     4441 accggtcagg taaccgccac cttcggttct ggtataaccg ggacgatggc aggcagaatc
     4501 agccgcagtt ccgatcacgt cgattttgat cacgccaaaa tcgataacag aaatggtacc
     4561 tttgccgatc ccagtcaagg catgaccggc cgcttctacg gcaaccaggc ggcagccatg
     4621 gccggctata ccacacgtgg taccggtaat gtcggcgatg acgtagcttt cggcggtagc
     4681 gccaccgata ttgagacccg caaataacag ctgccatccg atttggtttg gcaacggcta
     4741 ggctcgattt aacccctgag gagtttcaag ctgccgtttt atgccgactg ccaagccata
     4801 taaatcatcc gtccgatacg gattgctgct cggtttcagg tagcccgtgt tcaacacagg
     4861 ctacctgaaa tttgatattt gtaaataaac atcgcctatc ccatacggtt tcggcctatc
     4921 ggacattttg ggacagcaat taaaacctgc gttactttat cttttcggaa ggcaaccatg
     4981 aacaaaccac tcagcctcgc cctcttctgc ctgcttctgc ccatcaccgc ccacgccgac
     5041 aaccccgccg acgaacgccg cctgctggac gagggcagcc gccaaacgca gcaataccgc
     5101 gaaagcggtt ggctggacac ggaacaggca cgcggggaag tagaggaaaa cgacggctat
     5161 atttcgatag gcggcgaaat ctaccaagtc ggcgatacgg cggaagaact ggaaagcgca
     5221 atttaccacg ccctcaatgc gcggcagtgg cacaaggtgc ggcagttcgc cgcccgctac
     5281 gccaaactgc cccggcacaa gcccgcgctg attcatttgg ccgatgcgct gcaaaaacgg
     5341 gacgaagggg atttccgcgc ggcggggaac agttttcaga cggccttgga agccgaaccc
     5401 gacaacccgc gcctgctgct cgaagcgggg cggttttatg ccgaagacaa ccaaaacaaa
     5461 gagtctgccg ccgcgtttga gaaagttctg aaaacggaca ttcccgcaga aacccgcccc
     5521 atcgtggaaa actacctgtc cgaactgggc aaacgccgcc gctggcacgg ccaaatcagc
     5581 ctcggctacg gctataacag caatgtaaac caaggcaacg gcatcaacca atgcgtgtgg
     5641 gaaattgcgg gcatgtgcct gatggagcgc accctgcccg cgccaacgga ttcgaccttc
     5701 tcatcctata gcgcgaccgc cgaaaaaacc gtcccgctca aagggcatca cggcgtacag
     5761 gtgcgcggtg tactgtacgg caaccgctac acggaaaaag acaaagattc ggcggccatg
     5821 ccggattacg gctatcgcaa cggttcgctg tacgcgggat acgcctatgc cgacgcacgc
     5881 tcctcctttt ccctgctgcc ttatttcgaa tacgacttcc gcaaccgcca cacccactac
     5941 cgcgcatggg gtgcggatgc cgactggtcg cgcacactct ccccgcactg gcgcattaac
     6001 tcacatgccg gagcaaaaaa gaccggatac ggcggacaaa gcaaaactta ttttgccgat
     6061 ttcaaacaat acgaactcgg cgcgggggcg gaattttcca tcacgccgaa aagcggcctg
     6121 cttgtaaatt tcgatgccgc acgcaaagcc tatcccgaaa aatcctcttc cagcaaagaa
     6181 tatacggctc ggctgggcgc gtataggctt ttttcgggcg gtacttattt aaacgccgtc
     6241 ctgctctacc gccgcagcct gtatgacgcg gcaagtttcg tcagcgataa caaacggcgg
     6301 agcgacaagc aatacatcat gatggctgcg gcgggttttc cgcaatggaa tatcaaaggc
     6361 gtatatcccg aactgcgttt caggcgaaca atcgcacaca gcaacgcggt gtattaccgc
     6421 taccgccaaa acgaatggct gttgggtttt aaatatcggt tttgaccagt cgggtgataa
     6481 aaaatgccgt ctgaaagggc gcttcctttc agacggcatt tgtccgtaat gtttacagat
     6541 gaggattcat caggttctcg ggggagagga tgcggttgag ttcttcttcg ctcaacaacc
     6601 cgcgttccag tacgacttcg cgcacgcctt tgccggtctg ggcgcagatt ttgccgacca
     6661 agtcgccgtt gcggtggccg atgtacggat tcagataagt caccagcccg atggagttga
     6721 aaacgtaacg ctcgcagatt tcgcggttga ccgtaatgcc tttgacgcat ttgtcggata
     6781 ggttgactgc ggcattgccc aagagggaaa tggtttcaaa catacattgg gcgatgaccg
     6841 gctccataac gtttaattgc agttgcccgg attcggcggc gaaggtaatc gtcgtatcgt
     6901 tgccgatgac tttgaagcag acttggttga cgacttcagg aatcacggga ttgactttgg
     6961 caggcatgat ggaagaaccg gcctgcaatt cgggcaggtt gatttctttc aaaccggcgc
     7021 gcggaccgga agagagaagg cgcaaatcgt tgcagatttt agagagtttg acggccgtgc
     7081 gtttcaatgc gccgtgtacc atcacatatg caccgcagtc ggaggtcgct tcgatcaggt
     7141 tttcagtcag tttgcaaggc aagccgctga cttcggagag ttttttgacc accagttcgg
     7201 cgtagccttt gggcgtgttc acgcccgtgc cgattgccgt tgcgcccaaa ttgacttcca
     7261 agagcagttg gcgggtgcgg tcaaggttga ggatttcctc ttccaacaat acttggaaag
     7321 attggaattc ctggcctgcc gtcatcggta cggcatcttg aagctgggtg cgacccattt
     7381 tcaaaacgtc tttaaacgct tcggctttgg cggcaaaggc gtttttcaat acggtcagtt
     7441 tgtcgagcaa ttcgccgatg ctgtaataca cggcaaggcg gaagcccgtg ggataggcat
     7501 cgttggtgga ttggctggcg ttgacgtgat ccatcggatt gacgatgtcg tagcggcctt
     7561 tctcatagcc caaggcttcc aatgcaaggt tggcgatgac ttcgttggtg ttcatattga
     7621 ccgaagttcc cgccccgccc tgatacacgt cggacgggaa ttggtcgagg cagcggttgt
     7681 tcagcagaac ttcgtcgcaa gccttttcaa tggcggcggc gatttcgggt tttactgcac
     7741 ccaattcgcc gtttgcctgc gcggttgctt ttttcaccat caccatactg cggacaaact
     7801 gcggcacgtc ggaaattttt tgtgtggaga ttttaaagtt ttcaatggcg cgcagggtgt
     7861 ggatgcccca atacacttcg gcgggaatct cgcggtcgcc caataaatcg tgttcgatac
     7921 ggacagtcat gtttttacct ttgtaagtcg gataattaat attgaaaaaa tgcgccatcg
     7981 gaaagatgcc gcagcaggac gaac
//
LOCUS       DAWTCI010000084         7823 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWTCI010000084 DAWTCI010000000
VERSION     DAWTCI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.084"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(2..535)
                     /locus_tag="WM481_001834"
     CDS             complement(2..535)
                     /locus_tag="WM481_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFA9081091.1"
                     /translation="MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIGRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPGSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(592..2118)
                     /locus_tag="WM481_001835"
     CDS             complement(592..2118)
                     /locus_tag="WM481_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFA9081092.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVLLDNI
                     LQIRHTPHNNGLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDNPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDKHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
     gene            complement(2230..3771)
                     /locus_tag="WM481_001836"
     CDS             complement(2230..3771)
                     /locus_tag="WM481_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFA9081093.1"
                     /translation="MIFDNNYHYNAMPIRLLVNIQDADSAGIQTASENNNGTRMGYNH
                     PMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPPDMQQAVVA
                     YTLTVALLIPLSSYLADKFGTKKVFFGSIAVFMLGSALCAASGSLFELALSRVVQGIG
                     GSMLVPIPRLTILRVYEKSKLLNAINYAVMPALIGPALGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSVAACLLLLAAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SVGIVAGALILKNWTFLIPASSGLHFAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RGTRP"
     gene            3919..4698
                     /locus_tag="WM481_001837"
     CDS             3919..4698
                     /locus_tag="WM481_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFA9081094.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKNTEYESKAVDCANRIIEFTHFLLAIGYKPEDKGEPVKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKV"
     gene            4695..5396
                     /locus_tag="WM481_001838"
     CDS             4695..5396
                     /locus_tag="WM481_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFA9081095.1"
                     /translation="MSARENILAKLKKAGALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNID
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            5393..6847
                     /locus_tag="WM481_001839"
     CDS             5393..6847
                     /locus_tag="WM481_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HFA9081096.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDVPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(6996..7415)
                     /gene="fitB"
                     /locus_tag="WM481_001840"
     CDS             complement(6996..7415)
                     /gene="fitB"
                     /locus_tag="WM481_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin FitB"
                     /protein_id="HFA9081097.1"
                     /translation="MILLDTNVISEPLRPQPNERVVAWLDSLILEDVYLSAITVAELR
                     LGVALLLNGKKKNVLHERLEQSILPLFAGRILPFDEPVAAIYAQIRSYAKTHGKEIAA
                     ADGYIAATAKQHSLTVATRDTGSFFAADVAVFNPWHD"
     gene            complement(7415..7651)
                     /gene="fitA"
                     /locus_tag="WM481_001841"
     CDS             complement(7415..7651)
                     /gene="fitA"
                     /locus_tag="WM481_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin FitA"
                     /protein_id="HFA9081098.1"
                     /translation="MASVVIRNLSEATHNAIKFRARAAGRSTEAEIRLILDNIAKAQQ
                     TVRLGSMLASIGQEIGGVELEDVRGRNTDNEVSL"
BASE COUNT         1888 a         1959 c         2230 g         1746 t
ORIGIN      
        1 ctcattctgt aaaaaacgta tgcgctccaa ggtagttttt ggcgtaaaac ggtgtggaga
       61 gtttttcggt tttgatggtt ttgccgctgc cgggggcatg gatgaattcg ccgttgccga
      121 tgtagagtcc gacgtgtgag tagcggtgtg cgccgccggt gttgaagaat acgaggtcgc
      181 cggccttgag gcggctgtcg gggattttgc ggcttgccgc cgccatgtcg cgggcggtgc
      241 gcggcagctt gacgttgagg gcgtttttgt aaacgaattg aatcatgccg ctgcagtcga
      301 agccggttgc ggtgctgctg ccgccccatt tgtagggcgt gccgatgagt ccgaggctgt
      361 ggagcatgag ttcctgcgag ccttgtgtgc ggccgatgtg gctgatgcgg acggcttgga
      421 tttgccggac tgtctgtttg ggtttcggtt ggcggtgttt gccggaggtc gtgccgcatg
      481 aggcgaggag cagtgcgccg agacagagga aaagggtttt gtcgggggaa aacatggttt
      541 tttcctttgc gggttcggat atgccgtctg aaggtgtttc agacggcatt tctataataa
      601 accttcgatg ggcagcaggg ataggatttt tgcggcgatg cgtttccaaa gtttggcttc
      661 gggttcgttc gggtaggttt ttcgggtggc gggatcgtgc cattgcaggc ggttgtgttt
      721 gtcgagggta acgcggtagg cgtattcggg tgtggtatcg gcgagggtgc gctccatctg
      781 ttctgcgatt ttggggcttt cgatgacgac gcccatttcg gtattgagcc gtgcggaacg
      841 ggggtcgagg ttgaatgagc cgatgaagat gcgtttgccg tccacaatga aggttttggc
      901 atgcaggctg gttacggagc tgccggtcag gcctttgtct tttgtggcgg ggacggcatg
      961 gttgggttgc agctcgtaga gtttgatgcc ggctttgagc agcggttttc ggtatttgac
     1021 gtagccggaa tggacggcgg caacgtcggt cgcctgtagc gagttggtca ggacggtaac
     1081 gtctatgccg tcctgcacca gttttgccag tgcgtctgtg ccggattttg tagggacgaa
     1141 atagggtgaa accagataga cgcttttttc gggctgtttg agcgcgtctt gcagcctccc
     1201 ggcaatcggc ggtttgcggc ggtcgcggtc gagtcctttt gcagggttgt cgctgatcag
     1261 gcgggtttgg acgctctgcc agtcgatgcg tcccgtctgt attttttggt agaggggcga
     1321 ctgttcgacg gtttcgcggt agcgcaggag cgcgtgtctg gatgtttcgt cgttgtatcc
     1381 gagtgcttga agacccttgc cgatgttgcc gctgcggatg atgcgcgtgg cgttgtgggc
     1441 ggaatggctt gcccagtagc ggtcgaagtc gtgcgatact tcgccgacga cgctgccggt
     1501 ggcgaggatg tccaggtcgg cgaaaacggt gtcctcaccg actttgaagt attcgtcgcc
     1561 gatattgcgt ccgccgagta tggtggcgcg gttgtcggcg gtaaaggatt tgttgtgcat
     1621 gcggcggttg aggcggggga agtcggtcag gtagccgagt gcgcgccatt tgcgtaggac
     1681 gaaggggttg aacaggcgca cttcgatatt gggatggctg tcgagggcga gcaggagatc
     1741 gtccaacccg cgcgtgttgt tgtcgtccaa cagcaggcgt acgcgcacgc cgcgttctgc
     1801 ggcaaggtac atgaggttga acagcagcct gccggaaatg tcgttgcgcc aaatgtagta
     1861 ttgcaaatcg aggctgtgtt cggcagattc gataagggcg gcgcgggcgg caaaggcttc
     1921 gtgggggtcg tcgagcaggt agatgtcgga tagcccgttg ttatgagggg tgtgccggat
     1981 ttgcaggatg ttgtccagga ggacaggttt ggaagtattg aaatgacggc tttccgtccg
     2041 ttcttccagt gggggcaacc atgaagaaca tgaacagaga aggaggcata aaagggaaat
     2101 gaggctgcgt gttttcatcg gggatatagt ttcagacggc attgcctgta ttttggggtc
     2161 tgtatgcatt gaaatgaagc atcataatct aaaggttgga atgggtaaaa gttttgtcgg
     2221 gatgcggggt taaggccgcg tgcctcttgt caggtttgca ccgtctgaaa cgtgcagacg
     2281 cttaaaaacc aacgacgagg caagggtgat gccgccgata ctgagcaggg tcatacggaa
     2341 ggcgaaatgc aggcctgaag aagccggtat cagaaatgtc cagtttttaa ggattaatgc
     2401 gccggcaaca atgcccacgc tgatggcaag ctgttggttg accgccatca ggctgttgcc
     2461 gctgccggtc tgttgcgggc gcaaatcggc gagggtcagt gtgttcatgg cagaaaactg
     2521 tagggagttg catgcgccga tcgccagcga gaggaaaatc caaatccaca gcggcgagtt
     2581 tccgtcaggc agggcgagca gcatgatgaa ggcggcaagc agcttggtgt tccaaagcag
     2641 taccgtgcgg tagccgaaac gtttcatgag cggtgcaatc agcggtttga ccagcagcga
     2701 agacagggcg acgggtgcga ccagccagcc cgacaggctt gcgccgaagc cgaaagcgac
     2761 ttgaaacatc aggggcatca gaaaaggaat cgagctgatg ccgagacggc tgaacagatt
     2821 gcccgccagt cccaaacgga aggtgcgtat cagaaacagg tcggcggaat aaatcggttt
     2881 ggacgcggtt ttcatatgtc ggaaataacg gcgtgcaaac agcagtccgc cgcacagcgg
     2941 taacagtgca aaatacggag gcagcgcgtg cgacaggctt tctgccgcaa gcagcagcag
     3001 gcaggcggca acggaaaagg tcagatagcc tttgaaatcc agagcggtat cgttgccttt
     3061 gacatcgggc atgatgttgc gtcccaatat gaaacccagc agaccgatgg gcaggttgag
     3121 caggaaaatc cagtgccacg aagcgtattc gaccagataa ccgcccgcca gaggtcccaa
     3181 cgcagggccg atcagtgcag gcatgaccgc ataattgatg gcgttgagca gcttcgattt
     3241 ttcatataca cgcaagatgg tcagacgcgg tatcggaacc agcatcgaac cgccgatccc
     3301 ctgaacgaca cgggaaagcg ccaattcaaa cagcgaaccc gatgcggcgc acaatgccga
     3361 tccgagcata aaaacggcaa tcgaaccgaa aaagactttt ttcgttccga acttgtccgc
     3421 caaataactg ctcaaaggaa tcagcagggc aaccgtcagc gtataggcaa cgactgcctg
     3481 ttgcatatcc ggaggcgatt cgttcaggtc ggcggcaatt tcgggcagtg cggtatttaa
     3541 aatggtcgca tccaacatct gcataaaaat ggcaattgcc agcagaagcg gcagccaagg
     3601 ggatggtgcg cgggcggaca gggtgttttt ttccataggg tgattgtacc ccatccttgt
     3661 gccgttattg ttttcagatg ctgtctgaat gccggcagag tcggcatcct gaatgttcac
     3721 aagcaaacga atcggcattg cattgtaatg ataattatta tcgaaaatca tcagagtaag
     3781 gtacagtaag cgttatgggg cagtttgtaa gaaaaaccgg attatttttt aaaattagac
     3841 ttgacccgca acggtcaatt acttaaagta aacgcttacc tttctacaga aaaaacgggt
     3901 ttcccgttat caaaaaatat gagcgcaacc attcccccaa aaatcatccg atacgacagc
     3961 aatccgacag atgtctattt tttcggcact tgcgtccttg atctttttat gcccgaagca
     4021 ggcatggatg ccattgccct aatcgagcag cagggcatac gcgtccattt cccgatggcg
     4081 caaagctgct gcggtcagcc tgcctattca tccggccatc cgaccgaagc cttcgatgtc
     4141 gccaaagcgc aactcgacct tttccccgaa aactggccga ttgtcgtacc gtccggctca
     4201 tgcggcggca tgatgaaaca tcactggcct accctgttta aaaatacaga atacgaatcc
     4261 aaagcggttg attgtgccaa ccgtatcatt gagtttactc atttcttgct tgccatcggc
     4321 tacaagcctg aagacaaagg cgagccagtc aaagtcgccg ttcacacttc ctgtgccgcc
     4381 cgccgagaaa tgaatgtcca tctttcaggc tggcagttga ttgacggtat ggaaaacgtc
     4441 gaacgcatcg tccacgacca cgaaagcgag tgttgcggct tcggcggcac attctccgtc
     4501 aaacaagccg atatttccgg cgcgatggta accgacaaag ttgccgcgct gaaagaaacc
     4561 ggcgcaaccg aaatcatcag cgcggactgc ggctgtatga tgaacatcgg aggcaaaatc
     4621 gccaaggacg agccggatat gccgcgtccg aagcatatcg catccttctt gttggaacgc
     4681 accggaggca aagtatgagc gcgcgcgaaa atattttggc aaaactgaaa aaagccggcg
     4741 cattgccgat ggaagagcct gcggtttttg attattaccg cgaaaagggc gtttcttggg
     4801 acagcgaagc cgagcgtctg aaacattggg ctgccgccat gcgcgcggtc aaaaccgaaa
     4861 tctattgggt gacgaaaagc aattggatgc aggttttccg cgaagcggca gaaggcaagg
     4921 gtttaaaaaa catcctgctg cccttggcga ccgaacacgg acaaattgcc cgtgccgcat
     4981 tggcgggcag caatatcgac ccgattgcct tcgagcgcga aatcgatact tggaaaaccg
     5041 agtttttcac gaacatcgat gcaggcttca gcggcgcgca atgcggcatc gcccgcaccg
     5101 gcacgctgat gctgttttcc agccctgaag aaccgcgtac tttgagcctc gttccgcccg
     5161 tgcatttctg cctgttcgat acgtccaaga tgtacaacga gtttcacaac gccgtcgaag
     5221 gcgaaaaact ggtggaaaac ggtatgccga ccaatgtatt cctgatttcc ggcccgtcca
     5281 aaaccgcaga catccagttg acgctcgctt acggtgcgca cggtccgcgt gatttggtca
     5341 tcctcgccat tttgcccgac cacatttccc ctgccgattt ggaggaaaac gcatgactac
     5401 gcaaaccatc aagttccaca tgaagccgga aactttcaaa caaaacgccg caatttccct
     5461 tcaagacaag cccttgcgta aaagcctgcg taccgcgatg gatatgctga tgaccaagcg
     5521 caaagccgtt ttgaccgacg aagaagagtt gcaaagcctg cgcgatttgt gcgaacacat
     5581 ccgtcagcgt tccctctcca aattacccgc gctgctggag cagttggaag aaaacctgac
     5641 taagttgggc gtgaaagtac actgggcgga aacccctgcc gaagcctgcc aaattatcca
     5701 cgacatcatc acagccaaaa acggcaagct gatggtcaaa ggcaaatcca tggtcagcga
     5761 agaaatcgag ctgaaccatt atcttgaagc aaaaggcatt aaggcggtgg aaagcgactt
     5821 gggcgagttt atcgtccaaa tggcaggcga aaaaccgacc catatcgtga tgcccgcaat
     5881 ccataaaacc aaagaacagg tcagcgaact gttccaccaa aacctcggca caccgcttac
     5941 agacgacgtg gaccaactga ccggattcgc ccgcaaagcc ctgcgcgata tttacagcac
     6001 tgccgatgtc ggcttgagcg gcgtaaactt cgccgttgcc gaaacgggca cgctgtgtct
     6061 ggtcgaaaac gaaggcaacg gccgcttgag tactaccgtt ccgcccgtac atatcgccat
     6121 tacgggtatt gaaaaagttg tcgccaagct gtctgatgtt ccgcctttgt acagtctgct
     6181 gccgcgttcc gccatcggtc agaacattac cacttatttc aatatgatta ccggcccgcg
     6241 ccgcagtgaa gaattagacg gtccgcagga aatgcacttg gttttgctcg acaacggccg
     6301 cagccaagct tatgccgaag accaaatgcg ccgcaccctg caatgtatcc gttgcggcgc
     6361 gtgtatgaac cattgcccgg tttatacacg catcggcggc gcggcatacg gcacaaccta
     6421 tcccggtccg attggcgaga ttatttcccc gcacctgttg ggtcttgatg ccacccgcga
     6481 cctgccgacc gcctgcacga tgtgcggcgc gtgcgtggaa gtttgtccgg tacgcatccc
     6541 gattaccgaa caaatgcaac gtttgcgcgt tgaagcacaa cgttcgccga ccgaaaccgt
     6601 gccgcacccc atccgagggc aaggcgcatc gcataccttc ggcgaacaaa tggcgtggcg
     6661 cacattcaac ggtattttca gcggtagcaa aacctaccgc gccttcggtt gggcagccac
     6721 caagttccgc aatctgaccc cgcgcaaaca gttgggttgg acgcaaaacc gcgtaccgat
     6781 gaaaccggcg aagaaaaccc tgcacgaact gatggcggaa aaaatgcgcc aaaaagaaca
     6841 ggcataaaaa gttgttcgca aaaataccgt ctgaaacccg aaaacagggc ttcagacggc
     6901 ctgaaaagca gcctgaacgc gcaaaaataa ataatgaaac ggaatgctaa gacgctttca
     6961 ggcagcctga cattaaggct gcttaaaagc gtcttttaat cgtgccacgg attgaacacc
     7021 gcgacatcgg ccgcaaaaaa tgagccggta tcacgcgtag caactgtcaa actgtgctgt
     7081 tttgcagtgg cggcaatata gccgtctgcg gcagcaatct ctttgccatg tgttttggca
     7141 taggaacgaa tttgcgcata gattgcggca accggttcat caaaaggcag aatccgcccc
     7201 gcaaataaag gcaaaatgga ttgttccaaa cgttcgtgca gcacattctt tttcttgcca
     7261 ttgagcaaca acgccacacc caaacgcaat tctgcaacag taatggcaga caaatacaca
     7321 tcttccaata tcaaactatc caaccatgcc accacacgtt cattgggttg tgggcgcaaa
     7381 ggttcggaaa tcacattcgt gtccagcaaa atcatcacaa agaaacctcg ttatcagtat
     7441 tacgaccgcg tacgtcttcc agctcaacac ctccgatttc ctgccctatt gatgccaaca
     7501 ttgaccccaa acgtacagtt tgttgtgctt tggcgatgtt atccaaaatt aagcggattt
     7561 ctgcttcggt actgcgccct gcggctcgcg cacggaattt gattgcgttg tgcgtggcct
     7621 cggataaatt tctaatcaca acagaagcca ttgcctttcc ttcctgaaaa aagtttttca
     7681 aatgctatca aaataaaaaa aatgatagca atctattttt taacgcctgc ggatgcctga
     7741 aaattcaggt ggccgcaggc gtttttttac atctaaaaaa acgtctcccc tccctctttc
     7801 tgataaataa aaaacagtcg ctc
//
LOCUS       DAWTCI010000085         7564 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWTCI010000085 DAWTCI010000000
VERSION     DAWTCI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.085"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(235..2385)
                     /gene="yccS"
                     /locus_tag="WM481_001842"
     CDS             complement(235..2385)
                     /gene="yccS"
                     /locus_tag="WM481_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HFA9081099.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYEALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLSRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSFKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIAGTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSSGTYLQKIAERLKTGETGDDIE
                     YRITRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPDMGPDDFQTALDTLRGELGTLRTRSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
     gene            complement(2502..3728)
                     /locus_tag="WM481_001843"
     CDS             complement(2502..3728)
                     /locus_tag="WM481_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFA9081100.1"
                     /translation="MNTDNLHDILDETVQVYSQKNKAESETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            complement(3892..4935)
                     /locus_tag="WM481_001844"
     CDS             complement(3892..4935)
                     /locus_tag="WM481_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFA9081101.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEA
                     ARRRAQNSESMSF"
     gene            5055..5741
                     /locus_tag="WM481_001845"
     CDS             5055..5741
                     /locus_tag="WM481_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFA9081102.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGREPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSRQRRSLMPPLQVCVEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            5741..6148
                     /locus_tag="WM481_001846"
     CDS             5741..6148
                     /locus_tag="WM481_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFA9081103.1"
                     /translation="MEIRVIKYTATAALFAFMVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPLSLEDYEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTR
                     DGKPLVERFKQEGFDCLEKQGLRRNGLSERVRW"
     gene            6197..6988
                     /locus_tag="WM481_001847"
     CDS             6197..6988
                     /locus_tag="WM481_001847"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFA9081104.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGCRIHIANRGAEKRERLGKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVGTLSRYLGG
                     SRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVCRSQEMERQYQ"
     gene            7137..7553
                     /gene="dksA"
                     /locus_tag="WM481_001848"
     CDS             7137..7553
                     /gene="dksA"
                     /locus_tag="WM481_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFA9081105.1"
                     /translation="MVKLTEQDILNWIGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVEAQERRERMKKQFAD"
BASE COUNT         1525 a         1668 c         2544 g         1827 t
ORIGIN      
        1 caggcgggaa tctagacatt caatgctaag gcaatttatc gggaatgact gaaactcaaa
       61 aaaccggatt cccactttcg tgggaatgac gggattttag gtttctgttt ttggttttct
      121 gtttttgagg gaatgacggt tcagttgcta cgcatttacc ctgcgcaaag ccttatccac
      181 tatcttgcaa cctgcctgac aatccgcccc cctttacaaa atgccgaaat tttttcaggc
      241 tgcgttttgg ggctgcctgt gcggaatttg tcggtaggcg cggtagtagg gttcgagctg
      301 ccgggcgatg agttggagct gttggaggag gatgtggctt tgtgttccgc tgctgcgggt
      361 gcggagggtg ccgagttcgc cgcgcagtgt atccaatgcc gtctgaaagt cgtcgggtcc
      421 catgtcgggc aggtgttgga agatgtgggc ggtgtgttcg gcggcaaggt ggaactgtgc
      481 ggtaaagtcg gggctgcatt cttcgtgcat ttcgctgcgg tatgcgccga gggcggagat
      541 gtagccggtc agggcgtagc cggttttgag cagggtaaag ccgggttgca ggctgtcggc
      601 gaattttgcg ggttcgctgc tcatgtcgga aagggtgctg ctgagggcgg cggtgtgttc
      661 gtgggcgcgg cggcgggtga tgcggtattc tatgtcgtcg ccggtttcgc cggttttgag
      721 gcgttcggca attttttgga ggtatgtgcc gctgctgcat acggcaaggg cggcggtgcg
      781 ttcgagcgtg aggtatttcc agtctggcca caggtagctg accgccgccc aggcaaggga
      841 tgcgccgata atggtgtcga tgatgcgcac gggcatggcg gcgtatacgt ccaaacctgc
      901 gagggagagg ctggtcagtg cctgaatggt gatgaagaag gtggaaaaac tgtatttgta
      961 ggtgcgggtc atgaaaaaga gggtggtacc ggcgatgacg atccagagtt tggtttcgac
     1021 ggagggggtg aagtagggga cgagcgagcc gacgattacg ccgagtacgg tgccggcgat
     1081 gcgttggtac acgcggcttt tggtggcggt gtagttgggt tggcagacga aaagggcggt
     1141 cagcagtatc cagtagccga ggttgaggtt gagggcttcg acgatggtgc aggcggcggc
     1201 aacgacgagg gacaggcgga cggcatggcg gaatacgcct gattcgaggt tcagctgcgg
     1261 acggattgcc tgccaggtgt ttttgaagct gccggtttcg agggcggcga tgcgggtgtc
     1321 gcccatgcgg tcgttttcgg cgggggagtc gctgtgtcgg agttggcgga actgctggtc
     1381 gacgctgccg aggttgtcga gaaggcggct caggtggcgg atgtcgggac tgtcgttgcc
     1441 gtctgaaagg aggcgcagcg attggcggca gccttcgatg gcgcgtccga ggcgtttgct
     1501 gtaaacgtag tctttgcttg cgcgcagggc ttgtgcggtg ttgcggcagg cttgtccctg
     1561 catttcgagc aggcggtgga tgcggaagat gatgtcggtg tttttgaatt tttcggacat
     1621 ctcttggtag tcgacgtggg cggagctgat gcgttcgtgg atgtcttggg cggcgaagta
     1681 gtagcgcagc attttggcgg tgcgcgggtg gcggtgtttg ccgcgcaaac ggtaaaacag
     1741 ggcggaacgg cattggttga aggcggtgat gacgccggtg ttgctcatgg cgaggtcgat
     1801 gtggcggttg cctatccagg ctgcctcatc ggggtcgaag aagtcggctt tggcttcgag
     1861 gtagccgccg agtgcttcgt aggcgttggc gacgctttct tggacggggc ggtggggcag
     1921 gatgatttgg aacaggatga tggcggtgct gtacagtacg gtgccgcata aaatcatgaa
     1981 ggggttggtc agccagtagg tttcgggggt gtaggtaagc gtggtgtagg tggcgacggc
     2041 gagtgcgccg aaggcgaagg tgcggtattt cagcccgacc gcgcctaaaa tggtaaagcc
     2101 gaaggtcatc agggtcatgg cgaggatgaa gggcagccct gtgccgaggg tgctttgcgc
     2161 cgtgagcgag gagagggtaa acagggcgac ggtggcgatg atgtttttca gccgtccggt
     2221 caggcggttg tccaaatcga ccaggccgcc ggcgatgatg ccgagtacga agggcatggc
     2281 gagcttgggt tcgcctagct gccagacgat ggaggcggcg gtaaagacac tggcgaaaac
     2341 gggaagcgag gtaatgagca gaggcttgag gagtggggtt ttcatggttt taccggttta
     2401 ttgttatgaa gtgaataaag tgtggcacat gaatggggcg gataaaatca tgccgtctga
     2461 aaacggggat gcggttttca gacggcattg ggttttgcgg atcaggaaat gaggttgaga
     2521 ccgttgaccc tgtcgtaaag gagttcgggc gttttgcctt ctttgtgcag ttggatgtgc
     2581 aatcgcaggt tgttggcgga aacggactgg cgcagggctt cttcgtaact gatgatgccg
     2641 tgacggtaca gttcgaaaag gttttggtcc atcgtctgca ttccgtcggt tttggcggtt
     2701 tccatgattt tactgatgtt catcaggtcg cccttcagga tgaagtcttg gatggcgggc
     2761 gtgttgatga gcaagtcgac aaccgccgtc ctgcccgttt tgtctttttt gagggcgagg
     2821 cgttggcaga tgatgccggt cagattgagg gcgatgtcaa tcagtatttg gttgtgctgt
     2881 tctttggggt agaagttgag tatgcgttcg agcgactgcg gcgcggtgtt ggcgtggagc
     2941 gtaaaaatgc acaggtggcc ggtttgggcg agctgcatcg cgtattccat actttccctg
     3001 ctgcggactt cgccgataca gaccacgtct ggggattggc gcatagcgtt ttgtaccgcc
     3061 gtctgccagt ttatggtgtc gacgccgatt tcgcgctggg taaagatgca gcggcgcggt
     3121 ttgtagataa actcaatcgg gtcttcgatg gtaacgatat ggccgggcag ggttttgttg
     3181 cggtgttcga gcatagtcgc catcgtggtg gatttgcccg aaccggtagg cccgacgata
     3241 atcagcagcc cgcgcggtgc gacggcgagg tctttgagtt tttcgggcaa gcccaattcc
     3301 cgcatttgcg ggatgacgtg gttgatgcgc cgcaaaacca aacctgcgct gccttggctg
     3361 tagtaggcgt tggcgcggta gcgcgtgccg ctgcgcgatt ggacggagta gttgatttcg
     3421 ccgtcgcgcc ggaatatttc cgattgttcg gcgttcatcg tcgatgcggc gatggcggcg
     3481 gtttcctcgc ccgtcagagc cttttgcggc tgcggggtta atgcgctgtt gattttcaac
     3541 gagggcggga atcctttgct gataaggatg tcggacgcgt tttgtgcttc tgcggtttcg
     3601 cacaggcggt cgagcagcgg gtggaagtgt gcgccgattt cggccggggt ttcggattcg
     3661 gctttgtttt tttgagaata cacttgaacc gtttcgtcca agatgtcgtg caggttatcg
     3721 gtattcatcg ttagcttctt ttcggtttaa gccttgcagt ttgcggcggc aggtttcaac
     3781 aggaaggcgg acgcttcctg ttcggaaggg tatgccgggc gggatgccgc gtcccgcccg
     3841 gcgtgtttgc gccttgtttt cccgccggta tggccggaag gcggttgtgt gtcagaaact
     3901 catactttcg ctgttttgcg cgcgtctgcg tgcggcttcc ggtgcgatca gcccttggcg
     3961 caccagcgat tgcagcgatt ggtccatcgt ctgcataccg ctcgcctgcc cggtttgcag
     4021 gacggagtta atctgcgtga ttttgttttc gcggatgagg ttgcggacgg cggggttggc
     4081 aatcaggatt tcgtgcgagg cgacacggcc gttgccgtcg tgcgttttca gcaggttttg
     4141 ggagatgacg gcggtcagcg attcggacag catggaacgc accatttctt tctctccggc
     4201 ggggaatacg tccacgatac ggtcgacggt ttttgccgcg ccggtcgtgt gcagcgtgcc
     4261 gaaaaccaag tgtccggttt cggcggcggt cagtgccaag ccgatggttt cggggtcgcg
     4321 catctctccg acaaggataa cgtcggggtc ttcgcgcaat gcggaactca gcgcgttggc
     4381 gaagctgagg gtgtgctggt gcagctcgcg ttggttaatc agggattttt tgctttggtg
     4441 gacgaattcg atcgggtctt cgatggtcag gatgtgtgcc ggctgggttt cgttgatgta
     4501 gttgatcatc gcggcaagcg tggtcgattt gcccgaaccg gtagggccgg taaccaatac
     4561 catgccgcgc ggcgattctg cgattttttg gaaaatgctc ggggctttca attcttccag
     4621 cgataagacg gtgctgggaa tggtgcggaa tacggcggcg gggccgcggc cggtgttgaa
     4681 ggcgttgacg cggaatcggg cgacgttggg cagttcgaac gagaagtcga cttccaagtt
     4741 ttgctggtag attttccgct ggtggtcgtt catcaccgaa gttaccatat tgccgacctc
     4801 ttccgcgctc atttcgggaa ggttgatgcg ccgcatgtcg ccgtgaaccc gaatcatagg
     4861 ggatatgccc gaactcaggt gaaggtcgga tgctttgttt ttagcgccga aggcgagtaa
     4921 gtcggtaatc tgcataatgc ggctctgttt agtataatgt ttcgattggt tggaatgatt
     4981 ctaacaacct tgattatacc gccctgactg gaggggtttc aactgtttaa tcatttttaa
     5041 ttaggggatg atctatgacg gtgttgcaag aacgttatcg tgaggtgtcc gaccgtatcg
     5101 gaaaattggt tctgcaggcg ggcagggagc cgcattccgt cagcctgatt gccgtcagca
     5161 agaccttccc ttcagacggc atccgcgaag tttacgctgc cggacagcgt gatttcggcg
     5221 agaactatat tcaggagtgg tacggcaaaa cggaggaatt ggcggatttg accgacatcg
     5281 tgtggcacgt catcggagat gtgcagtcca acaaaaccaa atttgtcgcc gaacgcgcgc
     5341 attgggtgca taccgtatgc cgtctgaaga ccgccgtccg gctgagcagg cagcgtcgct
     5401 ccttaatgcc gcccttgcag gtgtgtgtcg aggtaaacat tgcgggcgag gcggcaaaac
     5461 acggtgtcgc gcccgaagaa gcggtcgcgc ttgccgtgga agtggcgaag ctgcccaacc
     5521 ttgtcgtgcg cggactgatg tgcgttgcca aagccaacag cagtgaaacg gagttgaaaa
     5581 ctcagtttca gaccatgcaa aagctgcttg ccgacctcaa tgcggctggc gttaaggcgg
     5641 acgtgctgtc tatggggatg tcggacgata tgcctgccgc cattgagtgc ggtgcgacac
     5701 acgtccgtat cggcagcgcg attttcggga aaaggggctg atggaaattc gggtaataaa
     5761 atatacggca acggctgcgt tgtttgcatt tatggttgca ggctgccggc tggcggggtg
     5821 gtatgagtgt tcgtccttgt ccggctggtg taagccgaga aaacctgccg ccatcgattt
     5881 ttgggatatt ggcggcgaga gtccgctgtc tttagaggac tacgagatac cgctttcaga
     5941 cggcaatcgt tccgtcaggg caaacgaata tgaatccgcg caaaaatctt acttttatag
     6001 gaaaataggg aagtttgaag cctgcgggtt ggattggcgt acgcgtgacg gcaaaccttt
     6061 ggttgagagg ttcaaacagg aaggtttcga ctgtttggaa aagcaggggt tgcggcgcaa
     6121 cggcctgtcc gagcgcgtcc gatggtaaaa aattgggaat gaatttagta aggtaatttt
     6181 gaatagggta gaaataatga atgtttattt tctcggcggc ggaaatatgg cggctgccgt
     6241 tgcgggcgga ttggtcaaac aagggggatg ccgcatccat atagccaacc gtggtgcgga
     6301 aaagcgcgaa cgtttgggaa aagagttggg ggtcgaaact tcggcaaccc tgccggagct
     6361 tcattccgac gatgttttaa tccttgccgt caaaccgcag gatatggaag ccgcgtgcaa
     6421 aaatatccgc accaacggtg cattggtgct ttctgtcgca gccggattgt cggtcggtac
     6481 gctcagccgt tacctcgggg gaagccgccg cattgtccgg gttatgccga atacacccgg
     6541 aaaaatcggg ctgggcgtat ccggtatgta tgccgaagcg gaagtatcgg aaacagaccg
     6601 caggattgcc gatcgaatca tgaaatcagt cggtttgacc gtttggttgg aagatgaagc
     6661 gcaaatgcac agcattaccg gcatcagcgg cagcgggccg gcttatgtgt tttatctgct
     6721 ggacgcattg caaaatgccg ccatccgaca agggtttgat atggcagaag cacgcgcgct
     6781 cagtctggca acgtttaaag gagcggttgc ccttgccgag cagacgggtg aagatttcga
     6841 gaagcttcaa aaaaatgtaa cgtcaaaagg cgggacaacc cacgaagccg tggaagcctt
     6901 cagacggcat cgtgtcgccg aagccataag tgagggcgtt tgtgcctgtg tgtgccgttc
     6961 gcaggaaatg gaacggcaat atcaataatg taaagaaaat aaaaaaaacc aatccaaaac
     7021 gtgttatgat gcgcgttttc aaaaacgcct taggcaataa gccttataaa aatcaaagga
     7081 ataaagccgc tttgtagtgc tttgtttttt tgcggtgaac cgagagggca tacattatgg
     7141 taaagctgac agaacaagat attttgaatt ggattgggcc ggaagacgat tatatgaatg
     7201 acgaccattt ggcttttttc cgcgaattgc tggtaaaaat gcaagacgaa ctcatcgaaa
     7261 acgcctccgt tacaacaggg catctccaag aacacgaatc agcccccgat cctgccgacc
     7321 gtgccacaca ggaagaagag tacgcattgg aactccgtac ccgggatcgg gaacgaaaac
     7381 ttctcagtaa aatacaggcg accatccgca atattgatga aggggactat ggattctgtg
     7441 ccgatacggg agaacctatc ggtctgaagc ggctgctggc acgcccgaca gccactttat
     7501 ccgttgaggc ccaagaacgc cgagagagga tgaagaaaca gtttgccgac tgatggaggc
     7561 aaac
//
LOCUS       DAWTCI010000086         7381 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWTCI010000086 DAWTCI010000000
VERSION     DAWTCI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7381
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.086"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..414
                     /locus_tag="WM481_001849"
     CDS             <1..414
                     /locus_tag="WM481_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9081106.1"
                     /translation="WLSAMSARLARFVPDEGERRRLLVNIQYESSRAGLDTQIVLGLI
                     EVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRYGCTILRHYRNLE
                     KGDIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            526..1908
                     /locus_tag="WM481_001850"
     CDS             526..1908
                     /locus_tag="WM481_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFA9081107.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAIYAAS
                     LPGAEDNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PMIADVAGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTRLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMHSIWLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFAGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNRDGLQRALLQLEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            1912..2436
                     /locus_tag="WM481_001851"
     CDS             1912..2436
                     /locus_tag="WM481_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFA9081108.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYDEGYGQSQEAYQRPAQQSRQPAPDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            2809..3732
                     /locus_tag="WM481_001852"
     CDS             2809..3732
                     /locus_tag="WM481_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFA9081109.1"
                     /translation="MENQRPLLGFALALLAAMTWGMLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWHSFRLLLLGVTGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLVGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFVYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRPFKRR"
     gene            complement(3797..5185)
                     /gene="fumC"
                     /locus_tag="WM481_001853"
     CDS             complement(3797..5185)
                     /gene="fumC"
                     /locus_tag="WM481_001853"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFA9081110.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYRPVHPNDHVNRAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHSLGRLNDAL
                     KGLYELALGGTAVGTGLNSHPEYAEIAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNENCAAGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNDKSLRETAVGLGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(5329..7308)
                     /gene="tkt"
                     /locus_tag="WM481_001854"
     CDS             complement(5329..7308)
                     /gene="tkt"
                     /locus_tag="WM481_001854"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFA9081111.1"
                     /translation="MSQLANVIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQTAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     AYPAFEIPQEIYDAWNAKEKGAKLEAGWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAAKAED
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGAYVISEAQGNAQAVIIATGSEVGLAVEAQK
                     VLAGQGIAVRVVSMPSTSVFDRQDAAYQAAVLPEGLPRIAVEAGHTNGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
BASE COUNT         1427 a         1887 c         2253 g         1814 t
ORIGIN      
        1 tggttgtccg cgatgtcggc acgtttggca aggttcgtcc ccgacgaggg ggagcggcgc
       61 aggctgctgg tcaatatcca atacgaaagc agccgggccg gtttggatac gcagattgtg
      121 ttggggctga ttgaagtgga aagcgcgttc cggcagtatg cgataagcgg ggtcggcgcg
      181 cgcggtctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      241 ctgttcgata tccgtaccaa cctgcgttac ggctgcacca tcctgcgcca ttaccgaaac
      301 cttgaaaagg gcgacatcgt ccgcgcgctt gcccgcttca acggcagcct cggcagcaat
      361 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      421 acccgtgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      481 gttcagacgg cattgcaact gttgatgccg tctgaaaaat tatttatggc aagagacaac
      541 cgcatccaaa tgtttccgca cgaatggcgc gccagtacga cgctttccgg cgtgtacgcg
      601 ctgcgtatgc tgggtatgtt cctcgtgctg cccgttttgg caatatatgc cgcctcgctg
      661 cccggtgcgg aagacaataa aacgctggtc gggctggcaa tgggcattta cgggctgacg
      721 caggcattgc tgcaactgcc tttgggtatc gcttccgaca agttcgggcg caagaaaacc
      781 atttatgtcg ggctggtcgt gtttgccgcc ggcagcttcc ttgccgccgc cgccgatacg
      841 ctgcccatgc tggttgccgc gcgcgccata cagggcgcgg gggcggtcag tgctgccgta
      901 accgcgctgc ttgccgactt gacgcgcgac ggcgtgcgta cccgcgcgat ggcgatgatc
      961 ggtttgagta tcggtctgac gttttcggtc agcctggtgg ttgcgccgat gattgccgat
     1021 gtggccggcg ttcgcggact gtttatgctg accggcattc tgaccgtcat cagcatcggc
     1081 gtagtggcgt ggatgactcc tgatcccgaa gtttccaagc tgcatgagga cacgcaggcg
     1141 cagccttcgc gcataggcga agttttgaaa aaccgtaggc tgctgacgct cgatttcggc
     1201 attttcgccc tgcacgccgc gcaaatggca ttgtttaccg cgctgccttt cgcgatgacc
     1261 cggctcggtt tggaaaaaat ccagcattgg aaagtatatc tgccctcgac cattacgggc
     1321 ttggtggtga tggttccgct gattatcgtc ggcgaaacgc gcaacaagct caaacaggtt
     1381 tttgttttgg gcatcgtctg catcgcggcg gcgcagcttg gtttgctgtc cggaatgcac
     1441 tcgatttggc tgattaccgc ttatctggtt gtttacttta tcggttttaa tgtgttggaa
     1501 gcgagcctgc cgtctatggt ttccaaaatc gcgccgtccg acctgaaggg tacggcgatg
     1561 ggcgtgtaca acacgatgca gtcgctcgga ctgtttgccg gcggcgcggc aggaggcttg
     1621 ctgtttcaaa aatacggctt tgccggcgtg tttgcctttt gcagcatatt gatgctgctg
     1681 tggctggtga ttgccgtttt atcgcccgcg cccaagcccg tcaaaaacct cagttaccct
     1741 gtcggcggcg tgtggcaggg caatcgggac ggtttgcaac gcgccctgtt gcagttggaa
     1801 ggcgtggaag acatcggttt cagtttcgac gggcagaccg tctatcttaa agtgttgcag
     1861 aagggtttcg atcaggctgc cgctgaaaaa atcattacag gagtttaaaa aatgtcattg
     1921 aacaaagtca tccttatcgg ccgcctcgga cgcgatcccg aagtgcgcta tatgcccaac
     1981 ggcgaggcag tgtgtaattt cagcgtcgcc accagcgaaa cttggaacga ccgcaacggc
     2041 cagcgcgtag agcgcaccga atggcacaac atcacgatgt accgcaaact ggcggaaatt
     2101 gccgggcaat acctcaaaaa aggcgggctg gtttatctgg aaggcagaat ccaaagccgc
     2161 aaataccaag gcaaagacgg catcgaacgc accgcttacg atattgtcgc caatgaaatg
     2221 aaaatgttgg gcgggcgcaa tgaaaacagc ggcggcgcgc cttacgatga aggttacggt
     2281 cagagtcagg aggcttacca acgccccgct cagcaaagcc ggcagcccgc tcccgacgcg
     2341 ccgtcccatc cccaagaagc acccgccgcg ccgcgccgcc aacccgtgcc ggccgccgcc
     2401 ccggtcgagg acattgacga cgacatcccg ttctgaattt tacggccgga catcctcgcg
     2461 gagggaagtc ataaaggacg gaaaaacctt aaaccttacg gggcaaggtt tctctttttt
     2521 gcatttgggc cggcttgttg ccgccccctg cgttgggcgt aacgttgcga ttgcgccgtt
     2581 cgggcgtttt gaggcaagcg atgcctgccg cactcccccg ctgttttcag acggcatttt
     2641 aaaattgagg cggtgttttc aaatagtgcg aagcaatagt ctggcttcag acagtttgtt
     2701 tgaaatcaaa gggttggaat caaacgccaa acgcttgaac catcattgcc cgatgcggca
     2761 aaccggcatc gtccggcaat acggttgttt aaaaataggg aaatcaggat ggaaaaccaa
     2821 aggccgctcc taggcttcgc gttggcactt ttggcggcga tgacgtgggg gatgctgccg
     2881 attgccgtgc ggcaggtatt gaagtttgtc gatgcgccga cgctggtgtg ggtgcgtttt
     2941 acagtggcgg cggcggtatt gtttgttttg ctggcattgg gcgggcggct gccgaagcgg
     3001 cgggattttt cttggcattc attcaggctg ctgctgctcg gcgtgacggg catttcggca
     3061 aactttgtgc tgattgccca agggctgcat tatatttcgc cgaccacgac gcaggttttg
     3121 tggcagattt cgccgtttac gatgattgtt gtcggcgtgt tggtgtttaa agaccggatg
     3181 actgccgcgc agaaaatcgg tttggttttg ctgcttgtcg gtttgcttat gttttttaac
     3241 gacaaattcg gcgagttgtc gggtttgggc gcgtatgcga agggcgtgtt gctgtgtgcg
     3301 gcaggcagta tggcctgggt gtgttatgcc gtggcgcaaa agctgctgtc ggcgcaattc
     3361 gggccgcaac agattctgct gttgatttat gcggcaagtg ccgccgtgtt cctgccgttt
     3421 gccgaaccgg cacacatcgg aagtttggac ggtacgttgg cgtgggtttg ttttgtgtat
     3481 tgctgcttga atacgttaat cggttacggc tcgttcggcg aggcgttgaa acattgggag
     3541 gcttccaaag tcagcgcggt aacaaccttg ctccccgtgt ttaccgtaat attttctttg
     3601 ctcgggcatt atgtgatgcc tgatactttt gccgcgccgg atatgaacgg tttgggttat
     3661 gtcggcgcac tggtcgtggt cgggggtgcg gttacggcgg cggtggggga caggccgttc
     3721 aaacgccgct agttcgcagg caacggaaaa tgccgtctga acgaggcttc agacggcatt
     3781 ttatttgagg gaaggattag cgcggatgaa ccatatcggc agggacgacc agttcgtcaa
     3841 actcttcgcc cgtcagcaag cccaacccaa cggcagtttc gcgcagcgat ttatcgtttt
     3901 tgtaggcggt tttggcgact ttggcggcgt tttcgtagcc gattttgcgg tttaacgcag
     3961 taaccagcat cagggaatgg tgcaggaaat agtcgatttt ttccggtacg ggttcgatgc
     4021 cggcggcgca gttttcgttg aagctgttgc acgcgtcgcc caagaggcgg atggattgca
     4081 agaggttgta agcgataacg ggcatataga cgttcagctc gaaattgccc gatgcgcccg
     4141 ccataccgat ggtaacgtcg ttgccgaaca cttggcagca caccatcgtc atcgcttcgc
     4201 attgggtcgg gttgactttg cccggcataa tggacgaacc cggttcgttt tcggggattt
     4261 tgatttcgcc caagccgcag cgcgggccgc ttgccagcca gcggatgtcg ttggcgattt
     4321 tgttcaggct tgccgccagc gttttcaatg cgcccgaagc ggcaacggcg gcatcgcgtc
     4381 cgcccaaggc ttcaaatttg ttcggcgcgc tgacaaacgg caggccggac agttcggcga
     4441 gtttggcggc ggctatttca gcgtattcgg gatggctgtt caaacccgta ccgaccgccg
     4501 ttccacccaa ggcgagttcg tacaaacctt tgagcgcgtc gttcagacgg cctaagctgt
     4561 ggtcgagttg ggaaacgtag ccggaaaatt cttgtcccaa agtcagcggc gtcgcatcct
     4621 gcaagtgggt acggccgatt ttgacgatgg gggcgaaggc ttgggctttt ttgtccaatg
     4681 tatcgcgcaa ggcttttacc gccggaataa ggtggcggtt gatttcaatc gcggcggcga
     4741 cgtggatggc ggtcgggaac gcgtcgttgg tcgattgcgc gcggttcaca tggtcgttgg
     4801 ggtggacggg ccggtacgcc gccaaacccg tacctgcaat ttcgttggcg cggtttgcca
     4861 gcacttcgtt catgttcata ttggactgcg taccggaacc ggtctgccaa accactaagg
     4921 ggaactgccc gtcgagcctg ccgttcagta catcatccgc cgcctgcgta atcaaatccg
     4981 cctgttcggg cttaatcctg ccgagggaaa cattggtcgc ggcggcggct tttttcacca
     5041 acgccaaagc gtaaatcagc ggctgcggca gggtttcgcc gccgattttg aaattgttgc
     5101 ggctgcgctg ggtctgcgcg ccccaatagg cttcggacgg gacttcgaca ttgcccatcg
     5161 tgtcgtgttc ggtgcgggtg ttcatacgtt tctccttttg aaatgtgaat aagagtgatt
     5221 cgcaaatatt ataatggaga ttggcggaat gaggaagcat caaggcggga gggcaaaaaa
     5281 atgccgtctg aaaagcattc agacggcatt tgatgcggcg gctgaggttt acagcacgga
     5341 tttcaccgta tcaaccacat tgtccacggt aaagccgaat gctttgaaca gcagatcggc
     5401 aggggcggat tcgccgaagc ggttgatgcc gacgactgcg ccgttcagtc cgacatattt
     5461 gtaccagccg ttggtgtgtc cggcttctac ggcgatgcgc ggcaggcctt ccggtaggac
     5521 ggcggcttga taggcggcgt cttggcggtc gaagacgctg gtggacggca tggaaacgac
     5581 gcgcacggca ataccttgtc ctgccaatac tttttgcgct tctacagcca atcctacctc
     5641 ggaaccggtg gcaatgatga cggcttgggc gttgccttgg gcttcactga ttacgtaagc
     5701 accgcgtttg atgtcgttca gttgttgctc gctgcgtgct tggaatttca ggttttgacg
     5761 gctgaaaatc aggcaggacg ggtggtcttc ggcttttgcc gcttctgccc aagcgaccag
     5821 agattctgcg gtgtcgcacg gccgccatac gtccatattc ggaatcaggc gcagggtggc
     5881 ggtttgctcg ataggctggt gggttgggcc gtcctcgccc aaaccgatgg aatcgtgggt
     5941 gaatacgaag acggggttga ttttcatcag tgccgccatg cgtaaggcgt tgcgttcgta
     6001 ttcgctgaac atcaggaaag tcgcgccgaa gggttttaca ccgccgtgca ataccaaacc
     6061 gttcataatc gcacccatac cgaactcgcg cacgccgtag tggatgtagt tgccgccttt
     6121 gtcgcgggta acggagacgc tgtttgacca gtcggtcagg ttggacgggg tcaggtcggc
     6181 agaaccgccc accagttcgg gcagctcttt tgccaagatt tcgatgctgt tttggctggc
     6241 tttgcgggtg gcgacggttt ccgctttggc gcacacttct ttcaatgccg tctgaacgta
     6301 ctcgtcaaag ttttccggca gctttttatc catgcggcgc acgaattctg cggcttcggc
     6361 aggatatttg gcttgatatt gcgcgaacag ttcgttccat ccggcttcca gtttcgcgcc
     6421 tttttctttg gcattccacg catcgtaaat ttcttgcggg atttcaaacg caggataagc
     6481 ccaacccaaa tgtttgcgcg tggcttcgat ttcgtccgca cccaaaggcg cgccgtgggt
     6541 tttgtggctg ccttctttgt tggcgctgcc tttgccgatt aaggttttgc agcagatgat
     6601 ggacggcttg ccggtttcgg cgcgggcggc ttcgatggcc gtctgaatgg cggcggtgtc
     6661 gtggccgttt acgttgggaa cgacgtgcca gccgtagctt tcaaagcgtt gcgggatgtt
     6721 ttcggtaaac cagccgtcca ctttgccgtc gatggagata ttgttatcgt catacaaaac
     6781 aatcagtttg cccaagccca aggtgccggc gagtgagcag gcttcgtgcg atacgccttc
     6841 catcaggcag ccgtcgccca taaagacgta agtgtagtga tcgacgatgt tcaaaccgtc
     6901 tttattaaat tcggcggcaa ggattttttc tgccaatgcc atgcccactg cgttggcaat
     6961 accttgcccc aacgggccgg tcgtggtttc cacgccgtcg gtgtagccgt attcgggatg
     7021 gccgggggtt ttgctgtgca gctggcggaa gtttttcaga tcttcaatgc tcaggttgta
     7081 gccggtcagg tgcagcaggc tgtacaacag catagacgca tgaccgttgg agaggacgaa
     7141 gcggtcgcgg ttgtagaatt tgggattggc gggattgtga ttgaggaatt tcgtccacaa
     7201 tgtttccgcc atttccgcca tacccatagg cgcgccgggg tggccggaat tggctttttg
     7261 aacggcatcg gccgagagga agcggattac gtttgccagt tgagacattt tgtattttcc
     7321 ttgctggtgt ttcggataag tggataatcg gaaggcgttg attatcgccc gattcgctta
     7381 t
//
LOCUS       DAWTCI010000087         7306 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWTCI010000087 DAWTCI010000000
VERSION     DAWTCI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.087"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(571..1035)
                     /locus_tag="WM481_001855"
     CDS             complement(571..1035)
                     /locus_tag="WM481_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFA9081112.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSSTPVTERVCRL
                     EREHYISGYHAHLNPHLLGKPPLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
     gene            1257..2315
                     /gene="alr"
                     /locus_tag="WM481_001856"
     CDS             1257..2315
                     /gene="alr"
                     /locus_tag="WM481_001856"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFA9081113.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTKGLEGEESLANSAAILNIPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRISMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(2857..4212)
                     /locus_tag="WM481_001857"
     CDS             complement(2857..4212)
                     /locus_tag="WM481_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFA9081114.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKHLSAKYGVPIVNQRISVTPIAQIAAATKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSVPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDAVSLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPAKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(4227..4499)
                     /locus_tag="WM481_001858"
     CDS             complement(4227..4499)
                     /locus_tag="WM481_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFA9081115.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEILDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(4624..5361)
                     /locus_tag="WM481_001859"
     CDS             complement(4624..5361)
                     /locus_tag="WM481_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFA9081116.1"
                     /translation="MKKLSRIVFSIVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(5362..5487)
                     /locus_tag="WM481_001860"
                     /pseudo
     CDS             complement(5362..5487)
                     /locus_tag="WM481_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
     gene            complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="WM481_001861"
     CDS             complement(5499..6398)
                     /gene="prmB"
                     /locus_tag="WM481_001861"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFA9081117.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAVYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIVPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINIEDYGLEERIQLIHTDLFEGLEGTYDLIVSNPPYVDAESVGALPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            6604..7215
                     /locus_tag="WM481_001862"
     CDS             6604..7215
                     /locus_tag="WM481_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081118.1"
                     /translation="MDNRKRRQGVRCLQCQRRDGRNRFARAFLPRCVDDTRASKWQVR
                     GCSRCRADRQAGERQPRDGNGRWTAGEVVSVSGGELGEFAETKELRKAAMQYARDNFV
                     GKSYVNESSGHELKVTWQGVKHAASKSNSIELSLMVALDKVLMNAKYEGSFPNKLGRD
                     HIIAVHKYSTGVRFGNKVLKIRIIAREKFDGIKHYDHFILKDK"
BASE COUNT         1965 a         1780 c         1667 g         1894 t
ORIGIN      
        1 ctaccgccct aaagggcagg gtttcaaccg aaaaggaaac acgatgaaag catatgattt
       61 tttgaataat cggattgacg ggtttttgag ggaatttgtt accggatagc catcgggcaa
      121 gttttttgca aaatttgaat cggtcgggta aattttcaaa aaataattga cagcagataa
      181 gaaacggcgg ataattaccg ccgttgagtt gcttgatgca gcttgatttt tctcctctac
      241 ttctcctttg tagacttggc acacattcaa ccggatgtgt gcatttttta tctccgcttt
      301 ttttgaaatt taattacttt tattgtttga aattctattc tttaagaaat ttaacaagaa
      361 aaacgcttgc tttttgaccg gaaaagctgc ataattcgtt ccgttagctg gttcgagtag
      421 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt ctattgaatg
      481 tgtgcatttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg tttcatttag
      541 atgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg gattttcttt
      601 gacttcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt gcaataggat
      661 attgccgagc atatcccgat aggcagacat atcgggcaaa cgtactttaa tcaggtagtc
      721 gtattcgccc gacaccagat ggcactccat aatttgcgga attttcagca cttctttttt
      781 gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa aaaccaatgg
      841 cggttttccc aacagatggg gattgagatg ggcgtgatag ccggaaatat aatgttcccg
      901 ctccaaacgg cataccctct ctgtaacggg cgtgctggac aaccctacct tctcggcaag
      961 ctccgtcatc gggatacggg cattttgctg aagaattttg aggatgcgga aatcgatttt
     1021 atctagttct ttcatttgga ttaccttgta tttattattg attttaacaa atagagtata
     1081 aatttgggaa agcaattata tcaggaaaag caaaccgcct tcctacctga aaactgctgc
     1141 tctggcttga agacacaagg ttctttaata ttttaaaagc cttgccgttg gattataatt
     1201 ccccaattga tttcttaatt ttgctaataa acacttgttt ggtaaggaat gaatttatgc
     1261 ggcctttgaa cgtgcagatc aggttgggca accttaggca caattatcgg attttgaagg
     1321 aaatgcacgg aggcaagctg ttggcggtag tgaaggccga cgcatacgga cacggtgcgg
     1381 tcagatgtgc tttcgcgctg gcagacttgg cagacggctt tgccgtggcg acaatcgacg
     1441 agggaatcag gctgcgggag agcggcatta cccatccgat tgtcctttta gaaggcgtat
     1501 ttgaagcatc agagtacgaa gcggtcgaac aatactcgct ttggccggca gtcggaaacc
     1561 aatggcagct tgaggctttg ctttcccgcc attggaaaaa acctgtcaag gtctggttga
     1621 aaatggattc gggaatgcac cgtaccggat ttttccctca tgattacgct tcggcatatg
     1681 cggcattgaa acagtcggaa tatgtggaca gtattgtcaa gttctcgcat ttctcctgtg
     1741 cggacgaacc tgaaagcggt atgacggaaa tacagatgga agcattcgat ttgggtacga
     1801 aagggctgga aggcgaagaa agccttgcca attcggcagc tattttgaat atccccgaag
     1861 cacgcaggga ttgggggcgc gcgggcttgg cgttatacgg catttccccg ttcggaggca
     1921 gcgatgacag gctgaaaccc gtgatgaggc tttcaacccg tattttcggc gaacgcgttt
     1981 tacagccgca ctcccctatc ggttatggcg caacatttta taccagtaaa tctacgcgcg
     2041 tcggtctgat tgcctgcggt tatgcggacg gttatccgcg ccgcgctcca agcaattccc
     2101 ccgtcgccgt cgacggtaaa ttgacccggg tcatcggcag gatctctatg gatatgatga
     2161 ctatcgagct ggacgcttcg caagaaggtt tgggacacga ggtcgaactg tggggcgata
     2221 cggtcaatat caataccgtt gccgaagcgg ccggaaccat cccttacgaa ttgatgtgca
     2281 atatcaaacg tgcaaaattc acttatatcg agtaatcgag tccaaacgaa aatcccgtct
     2341 gaagcttttc agacggcata aatgcgtttt gacaaaatca gtaaaagaca ttgccaaata
     2401 gaaaatactg ataatgccca caatctgaaa ccccatcctt cccgcaaaaa cggaaaatca
     2461 aaatcagaaa cctaaaattt cgtcattccc gcgcaggcgg gaatccggtt ttttgagttt
     2521 ccgtcatttc tgataaattc tcgtcgtttt tcatttctag attcccgcat gcgcgggaat
     2581 gacaaaatta aagtttcaga atttatttga acaacaaact agattcccgc tttcgtgggg
     2641 atgacggcgg ataggttttg ttgtttcgga taaattcctg ttgttttttg gtttccagat
     2701 tcccaccttt aaaagaatga cgtaagaaag cagaaataag aacacgggga ttttttaaat
     2761 ttgcaatatt cttttctaaa gacattttac ccaatgccca taaacaaaaa tgccgcctga
     2821 agcctttcag acggcatttt cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     2881 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcac gaaccttctt ttgccggcat
     2941 tacaggcgcg tagcccaaca gaccgccgaa ctcgacgctg tcgccgacgg ttttgcccgt
     3001 taccggaata atgcgcacgg cggtggtttt gctgttgatc atgccgatgg cggcttcgtc
     3061 ggcgatgatg ccggaaatgg tgtgcgcggg cgtgtcgccg ggaacggcaa tcatgtccaa
     3121 accaacggag cagacggcgg tcatggcttc gagtttgtcc agcgtcaaca cgcctgcctc
     3181 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccgc tcaaaccgcc
     3241 gaccgcgctg gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     3301 tgctgttgtg ccgtgcgtac cgcagacgct caagcccatt tcttcaagaa tgcgcgccac
     3361 cgagtcgccg acggcggggg tcggtgccag cgacaaatcg agaatgccga acgggatatt
     3421 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtgattt tgaaagcggt
     3481 tttcttcacg acttcggcga cctcggtcag gctgaccgcg tccgaatttt ccagcgcggc
     3541 tttgaccacg cctggaccgg atacgccgac attaatcaca gcatccgctt cgcccgagcc
     3601 gtggaacgca cccgccataa acggattgtc ttccaccgcg ttgcagaaca cgacgatttt
     3661 ggcgcagccg aaaccttcgg gcgtgatttc agccgtgcgt ttgatggttt cgcctgccag
     3721 cttgaccgca tccatattga taccggcacg cgtgctgccg atattgatgg agctgcacac
     3781 gatatcggta gttttcatcg cttcgggaac ggaacggatc aacacctcat ccgaaggcga
     3841 catacctttt tgcaccagcg cggaaaagcc gccgataaag gacacgccga tggctttggc
     3901 tgccttgtcc aaagtctgcg ccacgctgac ataactgtcg gctttggtcg ccgccgcgat
     3961 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcgga
     4021 aaggtgtttc gccgttgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     4081 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     4141 ggtgcggaca tcaaaattct ggtcggcaac cattttgacg gtttctaaaa tttcgccgga
     4201 ttggatactc atcacattcc tccgactcaa atgcggtgca tcgcttggaa gatttcttcg
     4261 ttttgcatac ggatgtcaag cgcgagtttt ttgctctctt ccgcaaacaa atccaaaatc
     4321 tcctgccgcg atttagggca tttcgaggta tccaccaaga taatcatagt aaaaaaatcg
     4381 tccatcagct gttggctgat attgaggatg ttgattcggt tttccgccaa aattttggaa
     4441 acatcgtaca cgatgccgac gcggtcttta ccgatgacgg tgatgactga attgttcaca
     4501 ggcttactcc ttgcagatat ccgttaaaat ccgaaattat accaccgtcg gattttgaag
     4561 aatattgtca acaatatata catacaaaat gccgtctgaa actatttcag acagcatcaa
     4621 gattcagggt tcgattaaat aaccatcctt atcccattgg gttttcctga ccaacttgtc
     4681 atcctgataa acagcctcgc tctttttaga accatcttca taccactcca aaaccacccc
     4741 gttgcgttta tggtggcgga tagacagttc cgaaagtaat cggccgcttt catcccaagt
     4801 cagaattttg gcaggctcat cgttgaccat aaccatttcc gtcttgatac ttccatcggc
     4861 ataccattgc ttccatacgc cgttcgcctt attttgctta aactggattt cgctttcctt
     4921 gccgccgtta cggtaataac ggtatcccgt accctcactc aagccatttt tataaggcat
     4981 aaccgcagat tttttaccgt tcggatacca gttgacccat tccccgtccg gcttaccctt
     5041 gctgaagccc cccgccattt ttttctgacc attaaaatgc cacaaaatca acataccgtt
     5101 ttgcagggta ggcacaaaag atttgatttg cgttgaagca acgatataag gttcggaata
     5161 tttcttcatc gacggataat aaaaatcctg cgcgtgtgcg atacccgccg ccacactata
     5221 ttgcctgata taagcggcag aagacatcgt cgccgtcagt ttcccgttct gattaaaata
     5281 aacagaatag gtctgcgccg gcaaagcggc cgaaaaaccc aacagtacga ttgaaaatac
     5341 aatccgagat aatttcttca ttatgatagc gatataaaaa acaattaact tataaacaat
     5401 atggtaagga tttctcaacc aagcgtcaaa cccgaaacaa tgtatcgtaa aaatgccgtc
     5461 tgaaaacaaa tcgtcttcag acggcatttc cctccaactc actcttcgcc caataactgc
     5521 tcgcgcgtca agaggaaaac aaaaccgtcg cccccgctgg tttccaacca agtaaaaggc
     5581 aactccggat acgctgcttc caatacatcc ctgttatgcc cgatttccac cagcaataca
     5641 cctttaggat tcagaaactt tgccgcattc agaagaatct gcctggtggc atccaacccg
     5701 tccgccccgc tgcccaacgc caattccggt tcgtgcaaat attcctcagg caaagcccca
     5761 accgactctg catccacata aggcggattg gaaacaatca aatcataagt gccttccagt
     5821 ccctcaaaca aatcggtatg aataagctga atacgctctt ccagaccata atcttcaata
     5881 ttgatccctg caacttccaa agcatccaaa ctcacatcaa ccgcatcaat ttgggcatca
     5941 ggataatggt gcgccatctg aatggcaagg caaccgcttc cggtgcaaag atccaaagca
     6001 ttatgcacca gctcatcgta ttctatccaa ggtcgaagtc cgtcacccag caattcataa
     6061 ataaaagagc gcggtacaat cacgcgttca tctacataaa aatcaaactc cccctgccag
     6121 gcctggtgtg tcaaataagc ggcaggaatg tgttcgacag cacgacgctc aataaccgcc
     6181 agcacttcct ctttttcagc ttccaagagt tttgcatcaa gatatggggc aagcatatcc
     6241 aaaggcaaat tcaaagtatg cagaatcaaa taaaccgctt catcatgcgc attatctgtt
     6301 ccatgaccaa aaaagagccc tgcctcatta aaacggctga ctgcaaaacg taaaacatca
     6361 cggatagtcg tcaattcttg tgcggcctga ttaaacataa tatgaaccat tctgcgtata
     6421 gatgctttta attataacag aaacaacaag caaacctttt catatcgcca aataaccact
     6481 caatctaccc atacaactac ataaatgccg cgcgaagacc attgcccgaa cggaaacggc
     6541 gatggcggac ggtatgggca acctgatagg ctgggaagaa acggggcttg ttgagggcaa
     6601 gcagtggata accgcaaaag acgacaaggt gtccgatgtc tgcaatgcca acggcgggat
     6661 gggcgtaatc ggtttgcacg agcctttctc ccacggtgcg ttgacgatac ccgagcatcc
     6721 aaatggcagg tgcgcggttg ttcccgttgt cgggcggaca ggcaagctgg cgaaaggcag
     6781 ccgcgcgatg gaaacgggcg atggacggca ggtgaggttg tttccgtatc gggcggcgaa
     6841 ttgggggaat ttgctgaaac aaaggagctt cgtaaagcgg ctatgcagta tgcgcgtgat
     6901 aactttgtcg gcaaaagcta tgtcaatgaa agcagcgggc atgaactgaa ggtaacttgg
     6961 caaggtgtga aacacgctgc gtcaaaatcg aacagcatcg aactttccct tatggtcgcg
     7021 cttgataaag ttttgatgaa tgcaaaatat gaaggaagtt ttcctaacaa attagggagg
     7081 gatcatatta ttgcggtaca caaatactca accggtgtca gatttggcaa taaagtctta
     7141 aaaatcagga taatagcccg tgagaaattt gatgggataa aacattacga tcattttatt
     7201 ttgaaagaca aatgaaaaaa ccctgtccag tatatctggg atagcttatg gaagcattta
     7261 acccagcctc ggataaggct ttaaatccat catgccattt atacgg
//
LOCUS       DAWTCI010000088         7277 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWTCI010000088 DAWTCI010000000
VERSION     DAWTCI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.088"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(125..1987)
                     /gene="hscA"
                     /locus_tag="WM481_001863"
     CDS             complement(125..1987)
                     /gene="hscA"
                     /locus_tag="WM481_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFA9081119.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDADGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRFIELHTRQGVKTPVEVSAEILKTLKLRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCYLLEQNRLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATFSDGMAIDTSISRAEFHNLTQHLVMKTFEPVKQALKDAGVGKNEVK
                     GVVMVGGSTRMPHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKADGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMGNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEEFAQI
                     QRDIADLQGRLKDGKAEDIRAAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            2158..3030
                     /gene="hpnD"
                     /locus_tag="WM481_001864"
     CDS             2158..3030
                     /gene="hpnD"
                     /locus_tag="WM481_001864"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFA9081120.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKQDAVTVLYVFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDNAFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCRRVAGVVGCLIARILGFSDGKTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            3105..4418
                     /locus_tag="WM481_001865"
     CDS             3105..4418
                     /locus_tag="WM481_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFA9081121.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAILATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGIADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLRRILPHLGEPEAVRVITEKRATTATDAPPPDL
                     SWLHRHRIFPAGDYLHPHYPATLEAAVQSGFASAEACLQSLNDAV"
     gene            4500..5219
                     /locus_tag="WM481_001866"
     CDS             4500..5219
                     /locus_tag="WM481_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9081122.1"
                     /translation="MPTLTDKTILVTGASQGLGEQVAKAYAAEGATVILVARHQKKLE
                     KAYDAIVEAGHPEPFAIRFDLMSAEEKEFERFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEARSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            5385..6056
                     /locus_tag="WM481_001867"
     CDS             5385..6056
                     /locus_tag="WM481_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFA9081123.1"
                     /translation="MKTVSAAIAFAAAAVSLTGCATESSRSLEVAKVASCNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDSEDRLGSQAKTILVTHLQQTNRFNVLNRTNLSALKQE
                     SGISGKAQNLKGADYIVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSTQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVDNLVQAVDNGA
                     WQSNR"
     gene            6066..6432
                     /locus_tag="WM481_001868"
                     /pseudo
     CDS             6066..6432
                     /locus_tag="WM481_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
     gene            6429..7076
                     /locus_tag="WM481_001869"
     CDS             6429..7076
                     /locus_tag="WM481_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFA9081124.1"
                     /translation="MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPL
                     NESPDVNGTWGMLASTAAPISEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVGAVVNQIANSLTDRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK"
BASE COUNT         1617 a         2123 c         2015 g         1522 t
ORIGIN      
        1 gcccttgatg acggttttca tcgaatagac aatgcctgac ttttccatat cgatgttttt
       61 caagtgtttt cgatccttca gacggcatca ggttatatct atgccgtctg aaaccgttta
      121 agtatcaaat attatcgaca ctctgacctg tcagcgcacg ttggatgttg cggttcatgc
      181 gcttggcggc gaaattgtcg gtgctgcggc tgagtttggc gacagctgca cggatgtctt
      241 cagctttacc gtctttcaga cggccttgca aatcggcgat gtctcgctgg atttgtgcaa
      301 actcttcggc atccagcaaa tcgctgtcca actcgagggc ggcgttgacg gcatcggtca
      361 ggctttcggc ttcgaccacg gcttcggcgc gggcgcgtgc cgccatatct tcggcggcgt
      421 tgcccatgct gtctttgagc atttgggtga tggtgtcatc gtccaaaccg taggagggtt
      481 tgacttcgat ttgcgcctgt acgccggtgc tttgttcttg ggcggaaacg gacagcaaac
      541 cgtcggcatc gatttggaag gtaacgcgga tacgcgccgc acccgccgcc ataggcggaa
      601 tgccgcgcag ggtaaatttg gcaaggctgc ggcagtcgga aacgagctcg cgttcgcctt
      661 gcacgacgtg tatcgtcatc gcggtctgac cgtctttgaa ggtggtaaag tcctgcgcgc
      721 gcgcggtggg gatggtggaa ttgcgcggga tgattttttc cgccaagccg ccgtaggttt
      781 ccaaaccgag cgacaagggg gtaacgtcca acagcagcca ttcgccgtcg gctttgttgc
      841 ctgcgaggac gtttgcctgt atggctgcgc cgagcgcgac gacttcgtcg ggattgaggt
      901 tgttcagcgg ggtttgtccg aaaaaggtgg cgactgcctg ttggacgtgc ggcatacggg
      961 tcgaaccgcc gaccataacc acgcctttga cttcgttttt accgacaccg gcatctttca
     1021 acgcctgttt gaccggctcg aacgttttca tcaccaaatg ctgcgtcagg ttgtggaact
     1081 cggcgcgact gatgcttgtg tcgattgcca taccgtctga aaacgtcgcc tgaatgcgcg
     1141 cttcggtttg cgtggtcaat tgttctttgg cggcgcggac gagcgagagc aggagttggc
     1201 tgtcttgttc gttgagttgg gagagtctgt tttgttcgag caggtagcag aacaaacgat
     1261 ggtcgaagtc atcgccgccc aacgcgctgt tgccgccggt ggctttgact tcaaacagtc
     1321 ctttggtcag ttgcaacacg gatacgtcga atgtgccgcc gcccaaatcg tacacgacaa
     1381 acgtgccttc cgaggcgttg tccagcccgt aggcgattgc ggcggcggta ggttcgttga
     1441 gcaggcgcaa tacgttcaaa ccggccagac gcgcggcatc tttggtggcc tggcgttgcg
     1501 cgtcgtcgaa ataggcgggg acggtaatca ccacgccgac caaatcgccg cccaaggttt
     1561 cttcggcacg caatttgagg gttttgagga tttccgccga cacttcgaca ggcgttttca
     1621 ccccctgccg cgtatgcagt tcgataaagc gttgattgtc gccgaaacgg taaggcaggt
     1681 aatgcgtatt ttgatgcaga tcggcaagag tccgcccgat aaggcgtttg gcggagctga
     1741 cggtgttcag cgggtcggtt ttttgggcgg acagggcggt tttgccgact tcaatgccgc
     1801 cgttttccag atagcggacg acggaaggca gggtaacgcg cccgtcggca tcgggcaggc
     1861 aggcggcact gccgctgcgg acggtggcga ccaagctgtt ggtcgtaccc aaatcgatgc
     1921 ctgccgccaa acggtgccgg tgcggggcgg cggacatacc cggttctgaa atctgcaaaa
     1981 gagccataaa ttttgcctgt cggtgcggtg tgtaaaggaa ggaaggcggc attttaacag
     2041 aatttgcgcc gatagttgag caaaccgcta aggattgggc cgtgccgccg aaattgccct
     2101 ctaccgccaa ataccgataa aatccgcctt tcccgcccat cattaggaat ataaatcgtg
     2161 aaaggactcg actattgccg ccaaaaagca gaggaaagcc gctccagttt tttgtcgggc
     2221 ttccgtttcc tgactcagga aaaacaggat gcggtaacgg ttctgtatgt cttttgccgc
     2281 gaattggacg atgtggttga cgaatgttcc aaccccgatg ttgcacaggc aacattgaac
     2341 tggtggcgcg gcgatttgga caatgcgttc ggcggcgcga tgccggaaca ccccgtcaat
     2401 caggccttgc ggcaggttaa ggaaaccttc aagctgccga aatatgaact ggaagcctta
     2461 atcgacggga tgcagatgga tttggttcag gcgcgttacg gcagttttga ggaattgaaa
     2521 ctgtattgcc gccgcgtcgc gggcgtggtc ggctgcctga ttgcgcggat tttggggttt
     2581 tcagacggca aaacgctgga atacgcggat aaaatgggtc ttgcgctgca actgaccaac
     2641 atcatccgcg atgtcggcga agatgcgcgc aggggacgga tttacctgcc gatggaggaa
     2701 atgcagcggt ttgacgtacc cgcaagcgtg attttgcaat gcagcccgac gggcaatttt
     2761 gccgaattga tggcgttcca aatcaaacgc gcccgtgaaa cctaccgcga agccgtatcg
     2821 ctgctgcctg atgccgataa aaaagcccaa aaagtcggac tggttatggc ggcggtttat
     2881 tacgcgctgt tgaacgaaat cgaccgagac ggcgcgcaaa acgtcctcaa atacaaaatc
     2941 gccctccctt cgccgcgcaa aaaacgcatt gccctgaaaa cctggttatt cggattcaaa
     3001 ccgcgccccg gcacgccgga acgggcataa ggcgcatact gcccttcagg aaaacccaac
     3061 ccccgccgcc ccgaaaaacg gggtttcaac cattggagga ataaatgatg aacacgccgc
     3121 atccgcgccc gaaaatcgcc gtcatcggcg caggctgggc cggcttgtcc gccgccgtca
     3181 ccttggcgcg gcacgccgac gttaccctgt ttgaagccgg ccggcaggcg ggcggaaggg
     3241 cgcgcacact ggccggaaat accgacggtt tcggtttttt ggacaacggg cagcacattt
     3301 tgctcggcgc ataccggggc gtgttgcgcc tgatgaaaac catcggttca gacccccgtg
     3361 ccgccttttt gcgcgtaccg ctgcactggc atatgcacgg cggtttgcag ttccgcgccc
     3421 tccccctgcc cgcgccgctg catattttgg gcggcgtgct gcttgcccgg cgcgtaccgt
     3481 ccgcattcaa agccaaactg cttgccgata tgtccgattt gcagaagtcc gcacgcctcg
     3541 gacagcccga cacgacagtt gcacaatggc tgaaacagcg gaacgtgccg cgtgccgccg
     3601 taatgcagtt ttggcagccc ttggtctggg gcgcgctcaa cacgcctttg gaaaccgcaa
     3661 gcctgcgcgt gttgtgcaac gttttgtccg acggcgtgct gacgaaaaaa tccggcagcg
     3721 actatctcct acccaaacag gatttgggcg caatcgtcgc cgaacccgcc ttggcggagc
     3781 ttcaacggct cggcgcggac atccgcctcg aaacgcgcgt atgccgtctg aacaccctcc
     3841 cggacggaaa agtcctcgtc aacggcgaag ccttcgatgc cgccatactt gccaccgcgc
     3901 cctaccacgc cgccgcgctc ctgcccgaag gcacgcccga acacgttcag acggcatatc
     3961 aaaaccttcg ctaccacgcc attaccaccg tctatctgcg ctacgccgaa cccgtccgcc
     4021 tgcccgcccc gctgaccggc attgccgacg gcacggcaca atggctgctt tgccggggca
     4081 ggctcggact gcccgaaaac gaagtctccg ccgtcattag cgtttccgac cgcgtcggcg
     4141 cgtttgcaaa ccgggcgtgg gcggacaaag cccacgccga cctcagacgc atccttccgc
     4201 atttgggcga acccgaagcc gtgcgcgtca tcaccgaaaa acgcgccaca accgcaaccg
     4261 atgccccgcc gccggatttg tcgtggctgc accggcaccg tatcttcccc gccggcgact
     4321 acctccaccc gcactacccc gccacgctcg aagccgccgt acaatcaggt ttcgcgtcgg
     4381 cggaagcctg cctgcaaagc ctgaacgatg ccgtctgaaa acgccggccg gcatcgggac
     4441 gctttacaag gtgcggcaaa acgctaaaat acattaaccg tcaaacccaa aggacaacca
     4501 tgccgacact gaccgacaaa accatcttag ttaccggcgc atcgcaaggc ttgggcgaac
     4561 aggtcgccaa agcctacgcc gccgaagggg cgaccgtaat tctggtggca cgccatcaga
     4621 aaaaactgga aaaagcctat gacgcgattg tcgaagccgg acaccccgaa cccttcgcca
     4681 tccgtttcga cctgatgagt gcggaagaaa aagaattcga acggtttgcc gccaccattg
     4741 ccgaagccac gcaaggcaaa ctggacggca tcgtccactg cgccggctat ttttacgccc
     4801 tctcgccgct ggatttccaa accgtcgccg aatgggtcaa ccaataccgc atcaacaccg
     4861 tcgccccgat ggggctgacc cgcgccctgt tcccgctgct gaagcagtcg cccgacgcgt
     4921 ccgtcatctt cgtcggcgaa agccacggcg aaacacccaa agcctactgg ggcggcttcg
     4981 gcgcgtccaa agccgcgttg aactacctgt gcaaagtcgc cgccgacgaa tgggaacgct
     5041 tcggcaacct gcgcgccaac gtcctcgtcc ccggccccat caattccccg caacgcatca
     5101 aatcccatcc gggcgaagcc agaagcgaac gcaaaagcta cggggacgta ctgcccgcat
     5161 ttgtctggtg ggcaagtgcc gaaagcaaag ggcggagcgg cgaaatcgtt tacctctaaa
     5221 tccggaaaag gcaggcatcc cgcccgcgcc gcgcttccgt tttgaaaaat atccgtacct
     5281 tccgcgcgca tccgtcccgc acagacaaac accttgactt aaatttacat aaggacaata
     5341 atgataaccc gttatttccc taactggacc ttagggtaaa ttttatgaaa accgtttccg
     5401 ccgccatcgc ttttgccgcc gctgccgttt cactgaccgg ctgtgcgacc gagtcctcac
     5461 gcagcctcga ggttgcaaaa gtcgcctcct gcaatacgca atatcacggt gttcgcaccc
     5521 cgatttccgt cggaacattc gacaaccgct ccagcttcca aaaaggcatt ttctccgaca
     5581 gtgaagaccg tctgggcagc caggcaaaaa ccatcctggt aacacacctg caacaaacca
     5641 accgcttcaa cgtactgaac cgcaccaacc ttagcgcatt gaaacaggaa tccggcattt
     5701 ccggcaaagc gcagaacctg aaaggcgcag attatatcgt taccggcgat gtaaccgaat
     5761 tcggacgcag agatgtcggc gatcatcagc tcttcggcat tttgggtcgc ggcaaatcgc
     5821 aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt caatacttcc gaaatcgtct
     5881 attccacaca gggcgcgggc gaatacgcac tttccaaccg cgaaatcatc ggtttcggcg
     5941 gcacttccgg ctacgatgcg actttgaacg gcaaagtttt agacttggca atccgcgaag
     6001 ccgtcgacaa cttggttcag gctgtcgaca acggcgcatg gcaatccaac cgttaaggag
     6061 aaatgatgaa tccgaaaacc ttgggccgtt tgtcgctgtg tgcggcggtc ttggctctga
     6121 ccgcctgcgg cggcgggcat aaaaacctgt attattacgg cggttatccc gataccgtct
     6181 atgaaggttt gaaaaacgac gacacttcgt tgggcaagca gaccgaaaag atggaaaaat
     6241 actttgcgga agccgccaac aaaaaaaatg aatgccgccc cgggtgcgca cgcccatttg
     6301 ggactgctgc tttcccgttc gggagacaaa gagggcgcgt tccgccaatt tgaagaagag
     6361 aaaaggctgt ttcccgaatc gggcgtattt atggacttcc tgatgaaaac cggtaaagga
     6421 ggcaagcgat gaaacctttg attttagggc ttgccgccgt gttggctctg tctgcctgcc
     6481 aagttcgaaa agctcccgac ctcgactaca cgtcattcaa agaaagcaaa ccggcttcaa
     6541 ttttggtggt tccgccgctg aacgagtcgc ctgatgtcaa cggcacttgg gggatgctgg
     6601 cttcgaccgc cgcgccgatt tccgaagccg gctattacgt cttccccgcc gcagtcgtgg
     6661 aggaaacctt caaagaaaac ggcttgacca atgccgccga tattcacgcc gtccggccgg
     6721 aaaaactgca tcaaattttc ggcaatgatg cggttttgta cattacggtt accgaatacg
     6781 gcacttcata tcaaatttta gacagcgtga cgaccgtatc cgccaaagca cggctggtcg
     6841 attcccgcaa cgggaaagag ttgtggtcgg gttcggccag catccgcgaa ggcagcaaca
     6901 acagcaacag cggcctgttg ggggctttgg tcggcgcagt ggtcaatcag attgccaaca
     6961 gcctgaccga ccgcggttat caggtttcca aaaccgccgc atacaaccta ctgtcgccct
     7021 attcccgcaa cggtatcttg aaaggtccga gattcgtcga agagcagccc aaataagcat
     7081 atgccgtctg aacctctccg agcttcagac ggcatatgcg aatcttgata cttgcaagcc
     7141 gcaccgccca aaatcaggcg accggtttca atatccgaac aaggttcggg ttctgtattt
     7201 tgcagaaccc gaacctttta ttcaaattta aacttaattc cggcgtggtt gtatggtaaa
     7261 ttatagtgga ttaaatt
//
LOCUS       DAWTCI010000089         7153 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWTCI010000089 DAWTCI010000000
VERSION     DAWTCI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.089"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            74..223
                     /locus_tag="WM481_001870"
     CDS             74..223
                     /locus_tag="WM481_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081125.1"
                     /translation="MNAEGFCEKQTACRPDGRRNAGDRADLKAFEFAGRELANYREML
                     KRYAV"
     gene            253..3300
                     /locus_tag="WM481_001871"
     CDS             253..3300
                     /locus_tag="WM481_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFA9081126.1"
                     /translation="MSDLEAKVRITVENHTKQGFDSAAADADKAAEKMRSSGDNAAKG
                     FKAAIDSMHETMRNLHADVKAGFEAAGNQAQQASEKVRAEVGKIGSGLSGLTKLLAGL
                     ATADFAKSVLDTADAMQLINSQVRQVTSSETEYLAVQQQLLDTANRTSASLESTANLY
                     VSTSRALKDYGYTQQEILKFTEATNNAMTIGGVGAQQQAVALMQLSQALGSGVLQGDE
                     FKSISEAAPILLDTIAEYMGKSRDEIKKLGSEGKLTADVIFKAISGTSEKFGEQAAKM
                     PVTMGQALTVFSNNWQSMVSKLLNDSGTMSGIAAVIKLIADNLNLVVPIVAGFAVAVA
                     AAVAPTLALNLALLANPFGIIAVAIGTVVGLIAKFGDEIDVFGGGWSNLSDVIRAVWQ
                     IITETVGEAVGTVKSWFDGLTGRLNEGAGGWPSLFGRVMSVISSAIGAYVNVYINIFA
                     TGWMLIKEAANDMPQFFANLGKAIGNVFISAIERMINKAVGMINSMIDFANKAASMAG
                     ISGIGKLNKVRMGRMDDGGLGERIHDSLTKDRAGAMANAVRERAADIHEADALKGRGG
                     GGHAKTARKKPGANQGGGKGGKSRSGGPGAAKDPMQAWEGEIKARKLAHREMQRETLT
                     HQEWDLAREAEYWRAKLATVDANGKTGVKIREKILTLEDQLSKQSTEAKMNQAAEWEK
                     LDKHKLEMEKDAADQALADGRISQLERLDLEIEFENRRYQIAYDALQERIALAEQDPA
                     YSQAAIGKLKAQMGELGRGHERTQAKNEGKRENQRRKDAPNVMEMLQDGGRNVWQEAQ
                     QQMGQAFTAMLARAQNFRQAMNGFFKSMGQTFIQEMVTKPLTGLMRRMVEESAIYKMI
                     FGTKETLETAAAAKTAATKATETTAVVSSNAVQAASGAAASQAHIPYVGPILAVAAMA
                     AMMSAVMGLMGGGGGSQTTTTTTRIPSAAGGWDIPAGINPLTQLHENEMVLPAEHAQT
                     IREMADQQGGGSTVIINSTGGDFIHKNDLAKLLKQMKRDFKFV"
     gene            complement(3360..3839)
                     /locus_tag="WM481_001872"
     CDS             complement(3360..3839)
                     /locus_tag="WM481_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HFA9081127.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(4181..5170)
                     /locus_tag="WM481_001873"
     CDS             complement(4181..5170)
                     /locus_tag="WM481_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFA9081128.1"
                     /translation="MATITQRNGKWRVQIRMKGVSRSATFERASDAKAWAARIESQIM
                     DGIQGNAPRNTIFADLIRRYLSEVTPSKRGAREESYRIGRALKTPLAKVRLADLRPQD
                     FADWRDQRLQEVSPTSVGRELTTLSAVCEHAMKEWGLLRENPVRKISKPKKSRARTRR
                     PTEQEIADICAALLYRPNEKPKMAVQRVAVAVLFAIETAMRAGEICGLKWADVNMRRR
                     IAHLPITKNGDSRDVPLSLRAAELIEQLRGIDDTWVFSLDAKSLDVLFRRARDNCGIQ
                     GLHFHDTRREALTRLSKKVPVEVLAKISGHRDLRILLNVYYRPDMADIAKMLD"
     gene            complement(5430..5618)
                     /locus_tag="WM481_001874"
     CDS             complement(5430..5618)
                     /locus_tag="WM481_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081129.1"
                     /translation="MADGRKGFFDAIIKINIFFFPVKTPTLGAANPIAGGSTRFRFGA
                     EQMAVLYVPFCVLKHIGR"
     gene            5859..6893
                     /locus_tag="WM481_001875"
     CDS             5859..6893
                     /locus_tag="WM481_001875"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFA9081130.1"
                     /translation="MSTSLSYRDAGVGIDAGDQLVEKIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYQNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPKNTVAQIDAESWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVRSFLSGQGETVYRLGCIRER
                     QGNEHQTQVA"
BASE COUNT         1806 a         1724 c         1982 g         1641 t
ORIGIN      
        1 tattccctgc gcgtcaaacc gccttcgtgt aaagcggttg caaaaaagcc ttttattttg
       61 acagggtgca gagatgaacg ccgaaggatt ttgtgaaaaa caaaccgcct gccggccgga
      121 cgggcgccga aatgcgggcg atcgtgccga tttgaaggcc tttgagtttg ccggacggga
      181 attggcgaat tatcgggaaa tgttgaaacg ttatgccgtc tgaaaagacg gcttttttat
      241 ttggggctgt aaatgagcga tttggaagcc aaagtcagga taacggtaga aaaccatacg
      301 aagcagggtt ttgattcggc cgccgccgat gctgataagg cggcggaaaa gatgcgcagc
      361 agcggcgata atgccgcaaa gggctttaaa gctgcaatcg acagtatgca cgagaccatg
      421 cgcaatttgc acgccgatgt gaaagcggga tttgaagcgg cgggcaatca ggcgcaacag
      481 gcgtctgaaa aggttagggc cgaagtgggt aaaatcggct caggcttatc ggggctgacc
      541 aaactgctgg caggattagc aaccgcagac ttcgccaaat cggtgcttga taccgccgat
      601 gcgatgcagt tgataaacag ccaagtccga caagtcacgt cgtctgaaac ggagtatctg
      661 gccgtgcagc agcagcttct cgatacggca aaccgtacca gtgcatcgct tgaatcaacg
      721 gcaaacctgt acgtttccac aagccgcgcg ttgaaagact acggctacac gcagcaggaa
      781 attttaaaat tcaccgaggc aaccaacaac gcgatgacca tcggcggcgt tggcgcgcaa
      841 caacaggccg tcgcgctgat gcagttatcg caggctttgg gcagcggcgt attgcagggc
      901 gatgaattta aatccatttc cgaagccgca cctattttgc tggataccat tgcggaatat
      961 atgggcaaat cccgcgacga aatcaaaaag ctgggcagcg aagggaaatt gacggcggat
     1021 gtgattttca aagccatttc cggcacgtcg gagaagttcg gggagcaggc ggccaaaatg
     1081 cccgtgacga tggggcaggc tttgacggtg ttctcgaaca actggcaaag catggtttca
     1141 aaactgctga acgacagcgg cacaatgtcg gggattgccg ccgttattaa actgattgcg
     1201 gataacctta atttggttgt tccgatcgtc gcaggttttg ccgttgccgt tgccgccgcc
     1261 gttgcaccga cactggcttt gaatcttgca ctgctggcaa acccgttcgg gattattgcc
     1321 gtcgcaatcg gcacggttgt cgggctgatt gcgaaattcg gcgatgaaat cgatgttttc
     1381 ggcggcggct ggtcgaatct ttccgatgtg attcgggcgg tttggcaaat catcacggaa
     1441 accgtcgggg aagcggtggg aaccgtcaaa tcgtggtttg acgggctgac aggccggctg
     1501 aacgagggcg cgggcggctg gccgtcgttg ttcgggcgcg tgatgagcgt catctcaagc
     1561 gcaatcggcg cgtatgtgaa cgtttatatc aacatattcg caaccggctg gatgctgatt
     1621 aaagaagccg caaacgatat gccgcaattc ttcgccaatc tcggcaaggc cataggcaac
     1681 gtgtttattt ccgcgatcga acggatgata aacaaagcgg taggcatgat taacagcatg
     1741 attgactttg ccaacaaagc cgcgtcgatg gccgggatat cgggcattgg aaagctgaac
     1801 aaggttcgga tggggcggat ggacgacggc gggcttggcg agcgtattca tgacagcttg
     1861 acgaaagacc gcgccggcgc aatggcaaat gccgtccgcg aacgggccgc cgatattcac
     1921 gaagccgatg ccctcaaggg acgcggcggc ggaggacacg ccaaaaccgc ccggaaaaag
     1981 ccgggcgcaa atcagggcgg cggcaaaggg ggcaaatccc gttcgggcgg tccgggcgcg
     2041 gccaaagacc cgatgcaggc ttgggaaggg gaaatcaaag cccgaaaact cgcacaccgc
     2101 gaaatgcagc gcgaaacgct cacgcaccaa gaatgggatt tggcgcgtga ggcggaatat
     2161 tggcgggcga agctggcaac ggtggacgcc aacggcaaaa caggcgtaaa aatccgcgaa
     2221 aaaatcctga cgcttgaaga ccaattatcg aagcagtcaa ccgaagccaa aatgaatcag
     2281 gcggccgaat gggagaaatt ggacaagcac aagctggaga tggagaaaga cgcggcagac
     2341 caagccctgg cagacggacg tatttcgcaa ctcgaacgcc tggacttgga aatcgagttt
     2401 gaaaaccgcc gttaccaaat cgcctatgac gcattgcagg aacgcatcgc acttgccgaa
     2461 caagacccgg catacagtca ggcggcaatc ggcaagctca aggcccaaat gggcgaattg
     2521 gggcgggggc acgaacggac gcaggcgaag aacgagggca aacgcgaaaa ccaacgccgc
     2581 aaagacgcgc ccaacgtcat ggaaatgctg caagacggcg gcaggaacgt ttggcaggaa
     2641 gcacagcagc agatggggca ggcgttcacc gccatgctgg cgagggcgca gaacttcagg
     2701 caggcgatga acggtttctt caaaagtatg gggcaaacct ttattcagga aatggttacg
     2761 aaacctttga cgggcttgat gcggcgcatg gttgaggaat cggccattta caagatgatt
     2821 ttcggaacta aggaaacgct ggagacagcg gcggcagcca agacggcggc aactaaggca
     2881 accgagacaa cggcggttgt cagtagtaat gctgttcaag cggcttcagg cgcggcggct
     2941 tcccaggcgc atatcccata tgtcggcccg attcttgccg ttgcggcaat ggcggcaatg
     3001 atgtcggcag tgatggggtt gatgggcggc ggcggcggtt ctcaaacgac cacaaccacg
     3061 acgcgcattc cctcggcggc aggcggctgg gacatcccgg caggcatcaa cccgctgacc
     3121 caactgcacg aaaacgagat ggtcctgcct gccgaacacg cgcaaaccat ccgtgaaatg
     3181 gcggatcagc agggaggcgg cagcaccgtc atcatcaatt cgacaggagg cgatttcatc
     3241 cataaaaatg acttggcgaa gctcttgaag cagatgaaac gggactttaa atttgtctga
     3301 gaaaatgccg tccgaaaggt tcggacggcc tatgttgcgg cgtacgtcaa gctattaagc
     3361 tatttggttt tcagaggctt tttcttccat ttccgggcta cggcttcaaa ttcttgaaag
     3421 gccgtcggcg cgctcctgat acggcgtatt tcaaaaatga agctgcttag gttgtcccag
     3481 tcgcgtaaca gcatggagta ttcgaaatct ttatacattt tctcgtgcag cgccccgccc
     3541 aatacgcccg cacagacaaa ttcgcgctgg tttaaaactg tcagtatggc ttcacggtct
     3601 ttcttcttgt ccggggtatc tgatgtatag gtggcgagta tgcatccgtc tgtttttgcc
     3661 agcccgttta ctatggttat ggcttcttga agggcggcat tgttacgttc ggccatgatc
     3721 atgtcgatgg tggcgcgctt tttggcgttt tccgtattcc tcatgatgcc gtaagcggca
     3781 acaaaaacgc cgataacagt caatatcggc gttgctatct gtattaggtt gtcagtcatt
     3841 gccgtcccag ccgtccgaaa ggcggaaatc acggctttcc gaataaatat gccccattgt
     3901 catcccctta agctgatgtt gagaataaat ccttgcgtcg gaatatacgg cattgcctga
     3961 ctattttcaa gattccgcac cccgcggacc ggactataca aaaaaaccgc cttcgtgtaa
     4021 agcggttgaa gagagccttt caataaaaat gccgtccgaa gggctttcgg acggcatttt
     4081 ttatagattc ttttttaatg aacctttgaa accgaagata attaattttc ctctttattt
     4141 tcaatacgca gtgtgcccta tgtttttaaa taaaaatatt ttaatccaac atttttgcaa
     4201 tatccgccat gtcggggcgg tagtaaacat tgagtaaaat ccttaaatcc cgatgcccgc
     4261 tgatttttgc caagacctcc acaggcactt tttttgatag gcgcgtgagg gcttcgcggc
     4321 gggtgtcgtg aaaatgcagg ccttgtatgc cgcaattatc ccgcgctcgc ctgaataata
     4381 catcaaggct tttggcatcc aggctaaaca cccatgtgtc atcaattccg cgcagttgct
     4441 ctattagttc ggcggctcgc aaggataatg gcacgtcacg gctgtcgccg tttttagtta
     4501 ttgggaggtg ggcaatacga cgccgcatat tcacatctgc ccattttagg ccgcagattt
     4561 cgcctgcccg catggcggtt tcgatggcaa ataggacggc aacggcaacc cgttgcaccg
     4621 ccattttcgg tttctcatta ggccggtata ggagggcggc gcaaatatcg gcaatttcct
     4681 gctcggttgg ccgtcttgtc cttgcccggc tttttttcgg cttgctgatt ttgcgtacag
     4741 ggttttcgcg aagaagtccc cactctttca tggcgtgttc gcagacggcg gataaagtgg
     4801 ttaattcgcg tccgacgctg gtgggggata cctcctgcaa ccgttgatct cgccaatcgg
     4861 cgaagtcttg agggcgcagg tcggcaagcc gcacctttgc caaaggtgtt tttagtgcgc
     4921 gtccaatgcg gtatgattct tcccgtgcgc cccgctttga tggtgtaacc tcggataaat
     4981 accgccggat aaggtcggca aagatagtat ttcgcggggc attgccttgg atgccgtcca
     5041 taatttgcga ctctattcgc gccgcccacg ccttcgcgtc tgatgcccgc tcaaatgtgg
     5101 cggaacggga aacgcctttc atacggattt gtacgcgcca tttgccgtta cgttgagtga
     5161 tggttgccat atttattcgg gtgcaattcg ggtgcaaata taatagagca aaacagatat
     5221 aaagcggggc aaaatagggc tatattaaat ttaatgcttt gatatgtagg gataaataga
     5281 tttaaaaaga cgaaagaagt gcggacgtag aataagcgta gatgcgctcc gtccgcacca
     5341 ataaaaatat ataaatcata ttgttaatga ttattgggac aattcggtgg cacaaaatat
     5401 aagctgtctg cttttcagcg ggcggctttt tatctgccta tgtgtttcaa cacacaaaac
     5461 ggcacataaa gcaccgccat ttgttccgca ccgaatcgga agcgggtgct tccgcccgca
     5521 atcggattcg ccgcccctaa tgtcggggtt ttaaccggaa agaagaaaat attgattttg
     5581 atgatggcat caaaaaaacc cttccgcccg tctgccaaaa ggaatggtta agaaaagcaa
     5641 tgcgtctttt gatggaacgc gatatataag aaattgaggg gaagggcagg cgggtttctg
     5701 cccgttaatc ctgcggacgg ccccgccaaa cccgacacgc gcgggcatat ggcggattgt
     5761 ttacaatatt gtctaaaaat ctgaacctgc aaacgcgaaa taaggtagaa tacgccccgt
     5821 tattttaccg tccaatcccc atttaccaag gaaaaacgat gagtacttca ttgagttacc
     5881 gcgatgcggg cgtcggtatc gacgcaggcg accaactggt cgaaaaaatc aaaccgtttg
     5941 ccaaacgcac catgcgtccg gaagtattgg gggatttggg cggttttggc gcattggtcg
     6001 aaatcggcaa aaaatatcaa aaccccgtat tggtcagcgg tacggacggc gtgggtacca
     6061 agctcaaact tgccttcgat tgggacaaac acgatacggt gggcatcgac cttgttgcaa
     6121 tgagtgtcaa cgatattttg gttcaaggag ctgaaccatt gtttttcttg gattattttg
     6181 cctgcggtaa attggatgtt ccgcgtgcga ccgatgtcat caaaggcatt gcccaaggtt
     6241 gcgaagaatc cggctgcgcc ctgattggcg gggaaactgc cgaaatgccg ggtatgtatc
     6301 ccgttggtga atacgatttg gcgggttttg ccgtcggcgt ggtggaaaaa gagaatgtca
     6361 ttaccggccg cagcatcggc gcgggcgatg tggtattggg tttggcttcc aacggcgcac
     6421 attcaaacgg ctattccctt atccgtaaaa tcatcgaacg cgacaatccc gatctggatg
     6481 ccgagtttga taatggcaaa actttacgtg aagccgttat tgcgccgaca cgtctgtatg
     6541 tgaaaccgat tcttgccgct ttggaaaaat ttaccattaa aggtatggcg cacattaccg
     6601 gcggcggcat taccgaaaac gtgccgcgcg tgttgcctaa aaacacggtt gcccaaatcg
     6661 atgccgaatc gtgggaattg cccaagctct tccaatggct tcaaaaggcg ggcaatgtgg
     6721 aaacccaaga aatgtaccgg acctttaact gcggcatcgg tatggtcgtc atcgttgctg
     6781 ccgaagatgc cgatgcggtt cggtcattcc tgagcgggca gggcgagaca gtgtaccgtt
     6841 tgggttgcat ccgagagcgt caagggaacg agcatcaaac ccaagttgcc tgacttccaa
     6901 gcatcaaatg ccgtctgaat ttgtttcaga cggcatttcc gcatcttcca aacgcgtatc
     6961 cgcaacacag ttctgcaaac tgatattttg cccggtatgg cggcttaaac ttcttcatca
     7021 tatttcgata atgctataaa atgatataat cataaaatat tgtttattcc tacatcgtat
     7081 tcatcctatt ttccgaaatg tgagcgtttt atgaagcaat ccgtccataa taaaacaatt
     7141 ttaagtgttt tga
//
LOCUS       DAWTCI010000090         7149 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWTCI010000090 DAWTCI010000000
VERSION     DAWTCI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.090"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(1019..1702)
                     /locus_tag="WM481_001876"
     CDS             complement(1019..1702)
                     /locus_tag="WM481_001876"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFA9081131.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDAFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            1851..4154
                     /gene="parC"
                     /locus_tag="WM481_001877"
     CDS             1851..4154
                     /gene="parC"
                     /locus_tag="WM481_001877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFA9081132.1"
                     /translation="MNTQPHASHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGNSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFMPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDRLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRIVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNIFLGDENEKRKLIIKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTAADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYSIDAAEIPGGRGDGVPISSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRLPV
                     ALIEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4174..5898
                     /locus_tag="WM481_001878"
     CDS             4174..5898
                     /locus_tag="WM481_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HFA9081133.1"
                     /translation="MVISNPRELEKLKDRIPNLINIIRVAIVFPLMIMHILGLETGSR
                     ANLHASWTAWAFYLWLAIACWLIFFSTLNPQWQWQWQALRIPSFSAVADITMIGVLTY
                     LFGGIDSGFGILILPFVGSSCLLSYGRYPLLYASYASILLIFNALADSNINMYPLILD
                     AKTVTNTFVVVAGSYFVAMIASLSVRYIDRAGKLAHENHVAYRRIRGLNQIVLNRVQE
                     AVVVINVEHQTILFNKKAKDLLPMLEIGQHTSLFDPVAILWDKTSSRTFEHYIDTPEL
                     TARIRAVPMNKKQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIR
                     HADGGTLFLDEVADLPLSMQVKLLRAIQEKAVRRIGDATEQPVDVRIVCATHKNLEAL
                     VESGAFRQDLYYRLNVVSLNMPSLREMRENLKLPTPYLLYKHSHNNRPYTLSPAAQQM
                     LLNYSYPGNFRELENILERAVALCVGYTVQIDDLQIQDVHHKPVRTETAVPVADTLPS
                     ETAAAPSRLLPFDPDTMQIQDYLDKIERDIIGQVLKQTEGNRTQAAKRLGISFRSMRY
                     RMERLNID"
     gene            5998..6750
                     /locus_tag="WM481_001879"
     CDS             5998..6750
                     /locus_tag="WM481_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFA9081134.1"
                     /translation="MTDILIDDTATEAVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSNVIVDFTSGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEVILLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGADKG"
BASE COUNT         1725 a         2113 c         1758 g         1553 t
ORIGIN      
        1 cccggcagct aaaaatcctg cgggatcggt gtggaattta gggataatct agtacagccc
       61 ctactatttt agtagtttta ccattagaaa aaggatatga atggaattgc cttgaggtag
      121 aagtactatc cgctgctcaa tctctagcaa atatctattg caacaaggtt cgcctcatag
      181 atggtcatgt ttgggaggaa taaatgaaaa agcgtagttg gtaattttta gttgcgcagg
      241 gtgggcttca gcccaccgtt ttttcttcat tttgggttgt tgattggtgg gctaaagccc
      301 accctacgat tactaaaatc ccgtcattcc cgcgtagagc gcatcataat aaggcgggaa
      361 tccagacctt tcggtgcgaa aacttatcgg ataaaacggt ttcccctgca tcctggattc
      421 ccactttcgt gggaatgacg ggatataggt ttccctacgg acgtgttcag attcctgcct
      481 tcgcgggaat aacggcggag cagtttctgc ttttccaata aatgaccgta acttaaaatc
      541 ccgtcatccc cgcgaaagca aaaatcctgc ctgtcggatt tcggtttttt cgggaaactg
      601 atgaatcgtc attcccgcgc aggcgggaat ccagaacgcg ggacggcggc aatattcaaa
      661 ggttgtctga aaattcagag gttccagatt cccgccttcg cgggaatgac agcggagcag
      721 tttctgcttt tccgataaat gaccgcaacc taaaacccat ccttcacacg aaaacagaaa
      781 aacaaaaacc taaaatcccg tcattcccgc gataacagtt gcgtaattgc gtagggtggg
      841 cttcagccca ccgtttcttc atttttgctt gtcaattagt ggactgaagc ccaccctgcg
      901 gttactgttt cttctttttc gattattaat tggcaggctg aagccctccc tacgtttacc
      961 gtcaaaaatg ccgtctgaaa tgctcaagcc ctcagacggc attttttatt tcgcaccctt
     1021 acaggtaaaa tttctcgatg actttcaggt tgtcgtctaa tttgtacacc agcggctggc
     1081 cggtcgggat ttccaagccc atgatgtctt cgtcggaaat gccctcgatg tgttttgcca
     1141 gcgcgcgcag ggagttgccg tgcgccgcta ccaagacgcg tttgccgctc aaaatcgcgg
     1201 gggcgatttg gtcttcccaa aacggtaata cgcgctccag cgttactttc aggttttcgc
     1261 cgtcgggtac gacatcggca ggcagatggg catagcgacg gtctttgtgt gcggaaaacg
     1321 cgtcgtcttt gtccaaaagc ggcggcaggg tgtcgtagct gcgccgccag atgcggactt
     1381 gctcgtcgcc gtatttttcg gcggtttgtt ttttgtccaa accttgcagt cggccgtagt
     1441 ggcgttcgtt cagcctccat gttttgattt gcggtacgaa cagttggtcg gattcttcca
     1501 aaacgatgtt gcaggtctta atcgcgcggg tcaggacgga tgtgaaggcg atgtcgaact
     1561 catagccgtt ttctttcagt tttttaccgg cggcggcggc ctcggcaagc ccctgctcgc
     1621 tcagcttcac gtcgcgccag cctgtaaaca ggtttttcgc gttccattcg ctttgtccgt
     1681 ggcggataaa taccaattcc atatcgtctc caatgtgtga aagtgggaaa gctttattta
     1741 taacatattt tcacatttcc cgtatttgat tcagattcag gcacggcgcc cactatggtt
     1801 tgccattttg atttacaata atgtcctttg ctttacattc cgcatacaca atgaatacgc
     1861 aaccgcacgc ttcccatacc gattccaaca cgctgatgct cggccgatac gccgaacgcg
     1921 cctatctcga atacgccatg agcgtggtca aaggccgcgc gctgcctgaa gtttcagacg
     1981 gccaaaagcc cgtgcagcgg cgcattttgt ttgccatgcg cgatatgggt ttgacggcgg
     2041 gggcgaagcc ggtgaaatcg gcgcgcgtgg tcggcgagat tttgggtaaa taccatccgc
     2101 acggcaacag ttccgcctat gaggcgatgg tgcgcatggc tcaggatttt accttgcgct
     2161 atcccttaat cgacggcatc ggcaacttcg gttcgcgcga cggcgacggg gcggcggcga
     2221 tgcgttacac cgaagcgcgg ctgacgccga ttgcggaatt gctattgtcc gaaatcaatc
     2281 aggggacggt ggattttatg ccgaactacg acggcgcgtt tgacgagccg ctgcaccttc
     2341 ccgcccgctt gcctatggtg ttgctcaacg gcgcgtcggg catcgcggtg ggtatggcga
     2401 ccgagattcc gtcgcacaat ttgaacgaag tcacgcaggc ggcgattgca ctgttgaaga
     2461 aaccgacgct ggaaaccgcc gacctgatgc aatatattcc tgctcccgat tttgccggcg
     2521 gcggtcaaat catcacgccg gcggacgaat tgcgccgtat ttacgaaacc ggcaagggca
     2581 gcgtgcgcgt gcgtgcgcgt tatgaaatcg agaaattggc gcgcggacag tggcgcgtca
     2641 tcgtaaccga actgccgccg aacgccaact ccgccaaaat ccttgccgaa atcgaagagc
     2701 aaaccaaccc gaaaccgaaa gcgggcaaga agcagctcaa ccaagaccgg cttaatacca
     2761 aaaagctgat gctggattta atcgaccgcg tgcgcgacga gtccgacggc gaacatcccg
     2821 tgcgccttgt atttgaaccg aaatccagcc gcatcgatac cgataccttc atcaacacgc
     2881 tgatggcgca aacttcgctg gaaggcaatg tgtccatgaa cttggtgatg atgggtttgg
     2941 acaaccgccc cgcgcagaaa aacctgaaaa cgattttgca ggaatggctg gatttccgca
     3001 tcgttaccgt aacacgccgt ctgaaattcc gtttaaacca agtggaaaaa cggctgcaca
     3061 tcctcgaagg ccgtctgaaa gtctttctgc acatcgacga agtgattaaa gtcatccgcg
     3121 aatcggacga cccgaaagcc gatttgatgg cggcattcgg gctgaccgaa atccaggccg
     3181 aagacatttt ggaaatccgc ctgcgtcagc tggcgcgttt ggaaggtttc aaactcgaaa
     3241 aagaattgaa cgaattgcgc gaagaacaag gccgtctgaa tatctttttg ggcgacgaaa
     3301 acgaaaaacg caagctgatt atcaaagaga tgcaggcgga catgaagcag ttcggcgacg
     3361 cgcgccgcac gctggtggaa gaggccggac gcgccgtgct gacacaaacc gccgccgacg
     3421 aacccatcac gctgattttg tcggaaaaag gctggatacg cagccgtgcc ggacataatc
     3481 tcgatttgag ccaaaccgcg ttcaaagaag gcgaccgcct caaacaaacc cttgaaggcc
     3541 gcactgtttt acccgtcgtc atcctcgatt catcgggcag aacctactcg atcgatgccg
     3601 ccgaaatccc cggcggacgc ggcgacggcg taccgatttc ctccttaatc gagttgcaaa
     3661 acggcgcgaa acccgtcgcg atgttgacag gattgccgga acaacattat ttattatcaa
     3721 gcagcggcgg ctacggcttt atcgccaagc tgggcgatat ggtcggacgc gtgaaagcgg
     3781 gcaaagtggt gatgaccgca gacagcggcg aaaccgtcct gccgccggtt gccgtctatg
     3841 cctcctcgtt catcaacccc gactgcaaaa tcattgcagc caccagtcaa aaccgcgccc
     3901 tcgccttccc catcggcgaa ttgaaaatta tggcgaaagg caaaggactg caaatcatcg
     3961 gattaaacgc cggcgaatcg atgacgcata ccgccgtttc ttccgagccg gaaatcctga
     4021 ttgaaagcga aggcaggcgc ggcgcggcgc acaaagaccg cctccccgtc gccctgattg
     4081 aggcaaaacg cggcaaaaaa ggcagactgt tgcccatatc gggcagcctg aaacagcttt
     4141 cttcccccaa ataaacccgg ttccgcacat attatggtga tttccaaccc ccgcgaactt
     4201 gaaaaactca aagaccggat tcccaatctg atcaacatca tccgcgtcgc catcgttttc
     4261 ccgctgatga ttatgcacat cctcgggctg gaaaccggca gccgtgcgaa cctgcacgcc
     4321 tcatggacgg cgtgggcgtt ttacttgtgg cttgccattg cctgctggct gattttcttt
     4381 tccaccctca acccgcaatg gcaatggcaa tggcaggctt tgagaatacc gagtttcagc
     4441 gccgtggccg acatcacgat gatcggcgtg ctgacctacc tgttcggcgg catcgattcg
     4501 gggttcggca tcctgatcct gcccttcgtg ggcagctcct gcctactcag ttacgggcgt
     4561 tatcccctgc tctatgccag ctatgcatcc atcctgctga tattcaacgc ccttgcagac
     4621 agcaatatca atatgtatcc gctgattctg gatgcaaaaa ccgtcaccaa taccttcgtc
     4681 gtcgtagccg gctcctattt cgtcgctatg attgcctcgc tgtcggtcag atacatcgac
     4741 cgtgccggca aacttgccca tgaaaaccac gtcgcctacc gccgtatcag gggcttgaac
     4801 caaatcgtgc tcaaccgcgt tcaggaagcg gtcgtcgtca tcaacgtcga gcatcagacc
     4861 atactgttca ataaaaaggc aaaagatctg ctccccatgc ttgaaatcgg acagcatacc
     4921 tccctgttcg accctgtcgc catcttatgg gataaaacat cctcacgcac cttcgaacac
     4981 tatatcgaca cgcccgaact gaccgcccgc atccgcgccg tgccgatgaa caaaaagcag
     5041 aacaagctgc tcatcctcta tatccgcccg caaagcgaaa ttcaggcgga agccctgtcc
     5101 gtcaaacttg ccgcgctcgg acaactgacc gccaaccttg cccacgaaat ccgcaacccg
     5161 atgtccgcca tccgccacgc cgacggcggc actttatttt tggacgaagt ggccgacctg
     5221 cccctttcca tgcaggtcaa actcttgcgc gcgattcaag aaaaagccgt gcgccgtatc
     5281 ggcgacgcga ccgagcagcc cgtcgatgtc cgcatcgtct gcgccaccca caaaaacctc
     5341 gaagcccttg tcgaaagcgg cgcattccgc caagacctgt attaccgtct caatgtcgtc
     5401 agcctcaata tgccgtccct gcgtgaaatg cgcgaaaatt tgaagctgcc caccccctac
     5461 ctcctgtaca aacacagcca caacaaccgg ccctacacac tctcccccgc cgcgcaacag
     5521 atgctcctga attacagtta tccgggcaat ttccgcgaac tcgaaaacat cctcgaacgc
     5581 gccgtcgccc tgtgcgtcgg atatacagtg caaatcgacg acctgcaaat ccaagatgtg
     5641 caccacaaac ccgtccggac ggaaaccgcc gtccccgttg ccgatacctt gccgtctgaa
     5701 acagccgccg caccgtcccg ccttctcccg ttcgaccccg ataccatgca gatacaggac
     5761 tatctcgaca aaatcgaacg cgacatcatc ggacaagtcc tcaaacaaac cgaaggcaac
     5821 cgcacgcaag ccgccaaacg cttgggcatc agcttccgct ctatgcgcta ccgtatggaa
     5881 cgcctcaaca tagactgaca aacggcatcc gcaccatctc cgcccacccg aaaaaatgcc
     5941 gtctgaaacg gcacggggaa gcgggttcgc cccacgaccg aacggacaca atacaccatg
     6001 accgacatcc tcattgacga taccgccacc gaagccgtcc gcaccctgat acgggcattc
     6061 cccctcgtgc ccgtttccca accgcccgaa caaggcagtt acctccttgc tgaacacgat
     6121 accgtcagcc tcaggcttgt cggggaaaaa agcaacgtca tcgtcgattt tacctccggc
     6181 gcggcacaat accggcgcac aaaaggcgga ggagaactca tcgccaaagc cgtcaaccac
     6241 accgcgcacc ccaccgtatg ggatgcaacc gcaggattgg ggcgcgacag cttcgtcctc
     6301 gcctcgctcg gactgaccgt taccgccttc gagcaacatc ccgccgtcgc ctgcctgctt
     6361 tcagacggca tccgccgcgc cctcctcaat cccgaaacgc aagacaccgc cgcgcgcatc
     6421 aacctccatt tcggcaatgc cgccgaacaa atgcctgccc ttgttaaaac acaaggcaaa
     6481 cccgacatcg tttatctcga cccgatgtat cccgaacgcc gcaaaagtgc cgccgtcaaa
     6541 aaagaaatgg cttatttcca ccggcttgtc ggcgaagcgc aagatgaggt catcctcctt
     6601 cataccgcac gccaaacggc aaaaaaacgc gtcgtcgtca aacgcccgcg cctcggcgaa
     6661 caccttgccg gacaagcccc cgcctaccaa tacaccggca aaagcacccg cttcgacgtt
     6721 tacctgccct acggagcgga caagggataa cgcccataaa acaagacacc gaaaaatttg
     6781 ccgttcttat gcaaacgaga aaccggtttt tgcgtttcga ctgttttgga taagtcatca
     6841 caccttaaag tttgtcattc ccacggaagt gggaatccga ttcgttcagt tttagttgtt
     6901 ttcaagtttc aggcaacttc caaaccgtca ttcccgcgaa agtgggaatc cggaaatgaa
     6961 aggcaacagg aatttatcgt aaatgactga aaccgaacgg actagattcc cgcctgcgcg
     7021 ggaatgacgg ggcgggagga tgccgtctga aattccgtca ttcccgtgaa aacgggaatc
     7081 tagaacttct gatttttcag acgacttttg aagattgccg ctacccaatg atctggattc
     7141 ccgcttttg
//
LOCUS       DAWTCI010000091         7129 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWTCI010000091 DAWTCI010000000
VERSION     DAWTCI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.091"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(498..1082)
                     /gene="rnhB"
                     /locus_tag="WM481_001880"
     CDS             complement(498..1082)
                     /gene="rnhB"
                     /locus_tag="WM481_001880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFA9081135.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKEQAVAWHVAASTPEEIASLNILHATMLAMKRAVYGLAARPEKIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRRPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1150..3045)
                     /gene="mnmG"
                     /locus_tag="WM481_001881"
     CDS             complement(1150..3045)
                     /gene="mnmG"
                     /locus_tag="WM481_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFA9081136.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRSQA
                     DRILYKASIREMLENQENLDLFQQAVEDVTLEGERISGVITAMGAEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGLNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYAPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAIPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGRVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3218..4645)
                     /locus_tag="WM481_001882"
     CDS             complement(3218..4645)
                     /locus_tag="WM481_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HFA9081137.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDCDNHHFRGGPAYYITHGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4804..5796
                     /gene="pdxA"
                     /locus_tag="WM481_001883"
     CDS             4804..5796
                     /gene="pdxA"
                     /locus_tag="WM481_001883"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFA9081138.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAHGEGGLEVLHIPAAEAVEAGWLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINAARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            complement(6026..>7129)
                     /locus_tag="WM481_001884"
     CDS             complement(6026..>7129)
                     /locus_tag="WM481_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFA9081139.1"
                     /translation="AWLKRIFGGSETQAVPAAETSEKRSTANRSGSRANNRRQNPRRS
                     KREGSKIEVREAAGKTAGQKARADKAETRNNGNRRRNERGDRATERANEAEIQSRNVQ
                     PAAPVADAAPPETEGQTGKRRRNGSRNERGQTAPETAAVAETAVQTAENTPPEPYTAE
                     DKGSKPKSERNRRERDSRDAKERRERNNQRDRRQNGKKRNIPSAAKIEQYLNIHDTAD
                     KVRSAAAHVFGETDANAPITVSIADPLIATPVQTASSAVSNGDALIYDAAEKIRRAAA
                     DILPEGAAPKAAAQEMPSETATFTAAAEQARETAQTGGLVLIETDPAALKAWAAQPEV
                     QAGRGLRRSEQPKPSEAATVPAEEMIQVETRQG"
BASE COUNT         1373 a         1888 c         2177 g         1691 t
ORIGIN      
        1 gtcattcccg caaaaacaaa aaaatcaaaa acagaaaact gaaatatcgt cattcccgcg
       61 caggcgggaa tctaggtctg tcggtacgga aacttatcgg gaaaaacggt ttttccaacc
      121 ctgagactcc ggattcctgt tttcgcggga atccggtttt ttgagtttca gtcatttttg
      181 ataaattctt gcagctttga gtttctagat tcccgctttt gcgggaatgg cggtttggaa
      241 gttacctgaa attcaaaaaa aaaacggaaa ccggacggat tggattcccg cctgcgcggg
      301 aatgacggat tttaggtttt tttgattttc tgtttttcgc gggaatgacg gttgggaagt
      361 ttcccgaaac ttaaaaacaa ctgaaaccca aaaaactaaa ttaccgcctg cgggggaatg
      421 acggttcggg ttctttctct ttgaagttgc gatgctggaa atgccgtctg aagttcagac
      481 ggcatttttg tgccggttta aaacaaggcc tgctgcgcga gcaggtttct gacgggggcg
      541 aagtcgcggc ggtgttcggg cagcacgccg tattgtttga gggcttccag atgctgcttt
      601 gtgccgtaac ctttgtgttt gtcaaaaccg tattggggac ggcgttgcgc cagtgcgtac
      661 atttccgcat cgcgtgcggt ctttgccaaa acggatgcgg cggagatttc gatgattttg
      721 ctgtcgcctt tgacgactgc ttcggcaggg atgcctaaat gttcgggaat gcggttgccg
      781 tcgatgaata ttttttcggg acgcgcagcc aaaccgtaaa cggcacgttt catggcgagc
      841 atggtggcgt gcaggatgtt gaggcttgcg atttcttcgg gcgtggaggc ggcgacgtgc
      901 cacgcgaccg cctgttcttt aatcatttcg gcaagcgcgt cgcgtttttt ctcgctgagt
      961 tttttggagt cggtcagtcc gggcaggtcg aatgtttccg gaaggatgac ggcggcggca
     1021 aacacgctgc cgaccaaagg tccgcgtcct gcctcgtcca cgccggcggt cagtatgtgc
     1081 atgatgtttc ctgtcgggat ggtacgatgc cgtctgaaaa tggtttcaga cggcatcgta
     1141 ccgatgtgtt tatttcgcgt ctttaaaccc gcgcttcaaa tgcaccatca gcagtgcgac
     1201 ggcggcaggc gtaacgccgg aaatgcggct tgcctgcccg acggtttcgg gtttgtgctg
     1261 gttgagcttt tgttgcactt ctgccgacaa gcctttgact ctgccgtaat cgatgccgtc
     1321 gggcagtttt aaggtttcga tgtcgcggcg gctgtcgatt tcttcgtttt ggcggtcgat
     1381 atagccttgg tatttgactt ggatttcgac ttgttcgacc acttcggcgg agaggcgttc
     1441 agacggtatc gcgccttcga gcgtcatcag cgcggcgtaa tcgaggttcg ggcggcgtaa
     1501 caaatcgtgc aggttggctt cgcggctgag tttttgtccg aacacgcgga tttgttcgtc
     1561 ttcggcgagt ttttgcggcg cgtaccacgt tgttttcaaa cgttggattt cgcgttcgac
     1621 ggcttcgcgt ttttcgttga acatgcgcca ttgcgcttca cccaccaagc cgattttgta
     1681 gccgtcttcg gtcaggcgca tatcggcgtt gtcttccctg agttgcaggc ggtattcggc
     1741 gcggctggta aacatccggt aaggttcgtt caggcctttg gtaatgaggt cgtccaccaa
     1801 tacgccgagg taggcttgtt cgcggcgcag caggagcgga tcttgtccgc ggacgtattg
     1861 cacggcgttc gcgcccgcca gcaatccttg cgcggcggct tcttcatagc ctgtcgtgcc
     1921 gttgatttgc ccggcgaaaa acagtccctc aatggttttg gtttcgaggc ttgctttgag
     1981 gttgcgcgga tcgaagtagt cgtattcgat ggcgtagccg gggcgcagga tatgggcgtt
     2041 ttcaagaccc ttcatactgc gcacgagcgc gatttggatg tcgaacggca ggctggtgga
     2101 gataccgttg gggtagtatt cgtgcgtggt cagaccttcg ggttcgagga aaatctggtg
     2161 gctgtctttg tcggcgaagc ggttgatttt gtcttcgata gacggacaat aacgcggacc
     2221 cacgccttcg attttgccgg taaacatcgg gctgcggtcg aagcctgagc ggatgatgtc
     2281 gtgggtttgc gtgttggtat gcgtaatcca gcaggacact tggcgcgggt gcatttcggc
     2341 gttgccgcgc acggacatga cgggaacggg cgtgtcgccg ggctgttcgg tgagttggga
     2401 aaagtcaatc gtgcgtccgt caatacgcgg cggcgtgccg gttttcagac ggccttgcgg
     2461 cagcttcaat tcgcgcaaac gtccgccgag cgatttggcg gctgggtcgc cggcgcggcc
     2521 gccttcgtag ttttccaaac cgatgtggat tttgccggac aagaacgtgc ctgcggtcag
     2581 caccacggcg cgtgctttaa actccgcgcc catcgcggtg atcacgccgc tgatgcgttc
     2641 gccctcgagt gttacgtctt cgacggcttg ttggaaaagg tcgaggtttt cttggttttc
     2701 caacatttcg cggatggagg ctttatacag gatgcggtct gcctgcgagc gcgtggctcg
     2761 tactgccgcg cctttgctgg cgttcaggcg gcggaattgg atacctgatt tgtcggttgc
     2821 caacgccatt gcgccgccga gcgcgtcgag ttcgcgtacc aaatgccctt tgccgatgcc
     2881 gccgatagag gggttgcacg acatttgtcc gagcgtttcg atattgtgtg tgagcaaaag
     2941 cgtctgcgcg cccatacggg cggcggcaag cgcggcttcc gtgccggcgt gtccgccgcc
     3001 gacgacgatg acatcgtagg ttttggggta aatcatgtgg gtcatgtgtg tattgcctat
     3061 cggtttttca gacggcattc caagcaggat gccgtctgaa aaacgaaacg gattcaaaag
     3121 taaagggttg ggattgtacg cctgttcgcc ctgtttttac agtgcgcgga aagggaaaag
     3181 ccgcttcgcg gggaagcggc tccggtaagg gtgggattta ccaaacatcg gatttgatgc
     3241 ggcgtttcag gcccggatgt tcggaaagtt tgaactcggg gtctttgccc attttcagct
     3301 tggcggtgta atcgcgcagc agcataaacg ccaatggcga gagcagcagg atggcgacga
     3361 ggttgatcca cgccatgatg cccatcgcca tatccgccat atcccagacc aaaggcacat
     3421 tggcaaccgc gccgaaatag acccacgcca aaaccagcat acggaaaacg gcggtaatca
     3481 gccaatggct tttgatgaat tggacgttgg actcggcata ggcatagttg ccgataacgg
     3541 tggaaaaggc aaacataaac aggatgacgg cgaggaaacc cgcgccccat tgccccactt
     3601 ggctgacaat cgccgcctgc gtcagcgccg caccgctcaa atcgccataa ggctgttggt
     3661 aaatcaagat gatgaaggcg gtgcaagaac aaacgatgat ggtatcgaca aacacgccca
     3721 gcatttgaat cataccttgc gaaacagggt gtttcacttc ggcggcggcg gcggcgttcg
     3781 gcgcggaacc catacccgcc tcgttggaat acaggccgcg tttgatgccc atcatcatcg
     3841 tttgcgaaat cagaccgccg agtaagccgc ctgctgccgc gtcgaatttg aacgcgcccg
     3901 aaaaaatctg accgaacacg tccggaatca tcggaatatt ggtcaaaatg atgaaaagcg
     3961 cgataaagag gtacaaaacc gccatcaggg gcacgacgat ttccgccgct ttggatatgc
     4021 gcctgatgcc gccgaagata atcggcgcgg ttaaaatcac cagggcgacg ccgacataat
     4081 gaggctccca gccccacgcc gctttgacgg tatcggcgat ggtattggtc tgaaccgctt
     4141 caaacacaaa accgaaacag aaaatcaggc tcagggcgaa caacacgccc agccatttct
     4201 gccccagacc gtgcgtgatg tagtaggcag ggccgccccg gaaatggtgg ttgtcgcagt
     4261 cgcggacttt aaagagctgc gccagcgaag attcgacaaa cgccgaactc atgccgatta
     4321 aggcggttac ccacatccaa aacaccgcgc ccggcccgcc gactttgatg gcgattgcca
     4381 cgcccgcgat attgcccacg cccacgcggc tggcaaggcc ggttacaaat gcctgaaacg
     4441 gcgtgatgcc gtgagggtcg tccccctgtt tgcggccgcc gagcatttct ttgatgctgc
     4501 gcccgaacag gcggaattgg acaaagcccg tggttacggt gaagaaaagc cccgtaccca
     4561 aaagcatata aaccaagtat gaccacatcg gatcgttgat ggcgccgacc cagccgtgca
     4621 gccattcggt aaagttctcg ttcatatcgc ttccttaaag ttgaaacccg cacatatggc
     4681 ggtatgcaag cagggtttaa attttgtaaa cgcccattct agcagattgt caacaaaatc
     4741 agaaaaattt acatcgccgc gcgcctgtgg cgttagaatc gcgttttgtt tggagcaaac
     4801 acgatgaaac agcctgtttt tgccgttact tccggcgagc ctgccggcat cggccccgat
     4861 atttgtttgg acttggcgtt tgcacgcctg ccctgccgct gcgcggtatt gggcgacaaa
     4921 cacctgttgc gcgcgcgcgc cgaagccttg ggcaaaagcg tcgtcctgcg cgacttcgat
     4981 ccggaatcag gcggcgcgca cggagaaggc gggctggaag tgctgcacat ccctgccgcc
     5041 gaagcggttg aggcagggtg gctcaatccc gccaacgccg cctatgtgct gcaacttttg
     5101 gacaccgcgc tcgcaggcat ttcagacggc attttcgacg gcatcgttac cgcgccgctg
     5161 cacaaaggca tcatcaacgc cgcgcgcgca agcacaggtt ttttcagcgg acacaccgaa
     5221 tatctggcgg aaaaaagcgg cacggggcag gtcgtgatga tgcttgccgg caaaggcctg
     5281 cgcgtcgccc tcgtaacgac ccacctgccg ctgaaagacg ttgccgccgc catcacgcaa
     5341 ccgctgattg aaagcgtcgc acgcattttg catcacgact taaaacacaa attcggcatc
     5401 aaaaatccca aaatcctagt cgccggactc aatccccacg ccggcgaagg cggacacctc
     5461 ggacacgaag aaaccgacac cattatcccc gcgctcgaaa acctgcgccg cgaagggata
     5521 aacctcgccg gcccgtatcc ggcggacaca ttgttccaac cgtttatgct cgaaggtgcg
     5581 gatgccgtat tggcgatgta ccacgaccaa gggctgcccg tgttgaaata ccacagcttc
     5641 gggcagggcg tgaacatcac gctggggctg ccctttatcc gcacctccgt cgatcacggc
     5701 accgcgctcg atttggcggc aaccggcagg gcggactccg gcagcctgat aactgccgtg
     5761 gagaccgccg tcgagatggc gcgcggcagc ctttaaagat gataaaagac ccgtcattcc
     5821 cgcgcaggcg ggaatccgga ccttcgggcg gcggcaatat tcaaaggtta tccgaaagtt
     5881 tgaggttcta gattcccgtt ttcacgggaa tgacgaaagg tggcgggaat cacgggaatg
     5941 gcgaagtttc agacggtatt gcaggtatcc gaaaccatgc aaaaagaggt tctgcggaac
     6001 agaacctctt tttgccgccg tcggttcagc cttgccgggt ttcgacttgg atcatttctt
     6061 cggcagggac ggttgcggct tcagacggtt ttggctgttc ggaacggcgc aaaccgcgtc
     6121 cggcttggac ttcgggttgt gccgcccatg ccttcaatgc ggcagggtcg gtttcgatca
     6181 ggacgagtcc gccggtttgt gcggtttccc gtgcctgttc cgccgcagcc gtaaaggttg
     6241 cggtttcaga cggcatttcc tgtgctgcgg ctttcggtgc cgcgccttcg ggcaggatgt
     6301 cggcggcggc acggcggatt ttttccgccg catcataaat cagtgcgtcg ccgtttgaaa
     6361 cggcggaaga tgccgtctga acgggtgtgg cgatgagcgg atcggcaatg ctgacggtaa
     6421 tcggcgcgtt tgcgtcggtt tcgccgaaaa cgtgcgcggc ggcggaacgg actttgtcgg
     6481 cggtgtcgtg aatattcagg tactgctcga ttttggcggc agacgggata ttgcgttttt
     6541 tgccgttttg acggcggtcg cgctgattgt tgcgttcgcg gcgttctttg gcatcgcggc
     6601 tgtcgcgttc gcggcggttg cgttcggatt tgggcttgct gcctttgtct tctgcggtat
     6661 acggttcggg cggcgtgttt tccgctgtct gaacggctgt ttcggcaacg gcggcggttt
     6721 ccggtgcggt ttggccacgt tcgttgcggc tgccgttgcg gcggcgtttt ccggtttgac
     6781 cttcggtttc gggcggtgcg gcatctgcaa cgggtgcggc aggctgtacg ttgcggcttt
     6841 ggatttccgc ttcgttggcg cgttcggtgg cacggtcgcc gcgttcgttg cggcggcggt
     6901 tgccgttgtt gcgcgtttcg gctttgtcgg cgcgcgcttt ctgtccggca gttttgcccg
     6961 ccgcttcgcg gacttctatt ttgctgcctt cgcgtttgct gcggcgcggg ttttggcggc
     7021 ggttgttggc gcggctgccg ctgcggtttg ccgtgctgcg tttttcggag gtttcggcag
     7081 cgggtacggc ttgggtttcg ctgccgccga aaatgcgttt gagccatgc
//
LOCUS       DAWTCI010000092         7120 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWTCI010000092 DAWTCI010000000
VERSION     DAWTCI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.092"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            154..1695
                     /locus_tag="WM481_001885"
     CDS             154..1695
                     /locus_tag="WM481_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HFA9081140.1"
                     /translation="MDGRRWAVRGAFSLLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMFKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFAQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFSVAVLSVCCLFPLSAIVVKAWSAGESRRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRLVWMRGLVFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDNGEGGK
                     RTETL"
     gene            1724..2323
                     /locus_tag="WM481_001886"
     CDS             1724..2323
                     /locus_tag="WM481_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HFA9081141.1"
                     /translation="MSELSEILSYNQKFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGVDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2320..2925
                     /gene="nadD"
                     /locus_tag="WM481_001887"
     CDS             2320..2925
                     /gene="nadD"
                     /locus_tag="WM481_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HFA9081142.1"
                     /translation="MKKIGLFGGTFDPIHNGHFHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRESATYTFDTVQIFRRQFPSAQLWW
                     LMGSDSLLKLHTWKKWQLLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLSAAGVSDGIPPVAARYIRKHGLYEK"
     gene            2984..3370
                     /gene="rsfS"
                     /locus_tag="WM481_001888"
     CDS             2984..3370
                     /gene="rsfS"
                     /locus_tag="WM481_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFA9081143.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTIWGGEKPSFHAGMQKPWHAAD"
     gene            3423..3893
                     /gene="rlmH"
                     /locus_tag="WM481_001889"
     CDS             3423..3893
                     /gene="rlmH"
                     /locus_tag="WM481_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFA9081144.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDAAYALKEIKPEKRG
                     AGVNAVQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAVSILHNHPYHRE"
     gene            3967..4233
                     /locus_tag="WM481_001890"
     CDS             3967..4233
                     /locus_tag="WM481_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFA9081145.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4390..5073
                     /locus_tag="WM481_001891"
     CDS             4390..5073
                     /locus_tag="WM481_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFA9081146.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSYISAALTLFISLYNIFSSLLN
                     ILLSLNGDD"
     gene            5203..5718
                     /gene="coaD"
                     /locus_tag="WM481_001892"
     CDS             5203..5718
                     /gene="coaD"
                     /locus_tag="WM481_001892"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFA9081147.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRNTYTAAERQDMLCAITDNFPNVRIEVFQNRFLVHYAREVDAGFIVRGIRSTSDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMIKGLVGPEGWMETVKRYVPPAVYQ
                     KMIAEHHNNNA"
     gene            complement(5857..>7120)
                     /locus_tag="WM481_001893"
     CDS             complement(5857..>7120)
                     /locus_tag="WM481_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFA9081148.1"
                     /translation="YSPKKYAVALGSSSTASGEYSYASGYNSVASGNKSYAAGYASVA
                     SAEGSVVIGDSRQVKPEADQGVAVGSKATVKNKAKQRVAVGSEAKVNAERGIAIGKEA
                     KAGGKTTNTLLDGPAYYADAIAVGYQAEAGKGGAIALGKQAKATKQNGMALGVESEAA
                     GDFSTAVGNESKAKGQGGVGLGNQSKAEADFAVAVGNKAEATKENSLVIGRYARANGN
                     HSVSLGSRSEIKDGVSNSVAPGYGSVASENNVVSVAYKETPQSTELSYRKIVGVDDGV
                     NDFDAVNVRQLKAMQGQNMAELFSVRSEVRGVAASSAALSALTPLSYDANNPTQFMVG
                     FGTYKGRQAMALGLSHFVNDRFMVKVGGTLGGNKTGHMMNVGLTWKFGTSQGVPATSD
                     KVLMEQLMQDNRQLKARLEKLEARLNAM"
BASE COUNT         1516 a         1905 c         1944 g         1755 t
ORIGIN      
        1 cctgcggccc gccgccttgt cctgattttt gttaatccgc tatatttcag acggcgggag
       61 gaaggcggat acggtacgta gtcggattga aatatacggt atgtggggct tttgggtaca
      121 atcgcaacat cagtgtttca agtggggaag gcgatggatg gacggcgttg ggcggtacgg
      181 ggtgcttttt ccctgctgcc ttcggctttt ttggcggtaa tggtcgttgc gcctttgtgg
      241 gcggtggcgg cgtatgacgg tttggcgtgg cgcgcggtgc tgtcggatgc ctatatgttc
      301 aaacgtttgg cgtggacggt gtttcaggcg gcggcaacct gtgtgctggt gctgcctttg
      361 ggcgtgcctg tcgcgtgggt gctggcgcgg ctggcgttcc cggggcgggc tttggtgctg
      421 cgcctgctga tgctgccgtt tgtgatgccg acgctggtgg cgggcgtggg cgtgctggct
      481 ctgttcgggg cggacgggct gttgtggcgc ggccggcagg atacgccgta tctgttgttg
      541 tacggcaatg tgttttttaa cctgcccgtg ttggtcaggg cggcgtatca ggggtttgct
      601 caagtgcctg cggcacggct tcagacggca cggacgttgg gcgcgggggc gtggcggcgg
      661 ttttgggaca ttgaaatgcc cgttttgcgc ccgtggcttg ccggcggcgt gtgccttgtc
      721 ttcctgtatt gtttttcggg gttcgggctg gcattgctgt tgggcggcag ccgttatgcc
      781 acggtcgaag tggaaattta ccagttggtt atgttcgaac tcgatatggc gggggcttcg
      841 gcgctggtgt ggctggtgtt gggggtaacg gcggcggcag ggttgctgta tgcgtggttc
      901 ggcaggcgcg cggtttcgga taaggcggtt tcccccgtga tgccgtcgcc gcctcaatcg
      961 gtgggggaat atgtattgct ggcattttcg gtggcggtgt tgtccgtgtg ctgcctgttt
     1021 cctttgtcgg caattgttgt gaaagcgtgg tcggccggcg aatcgcggcg tgtgttaatg
     1081 gaaagtgaaa cgtggcaggc agtgtggaat actttgcgct tttcggcggc ggcggtgttt
     1141 gcggcggcgg ttttgggtgt ggtgtatgcg gcggcggcgc ggcggctggt gtggatgcgc
     1201 ggactggtgt ttttaccgtt tatggtgtcg ccggtttgtg tttcggcggg cgtgctgctg
     1261 ctttatccgg ggtggacggc ttcgttaccg ctgctgctgg cgatgtatgc gctgctggcg
     1321 tatccgtttg tggcaaaaga tgttttatcg gcctgggatg cactgccgcc ggattacggc
     1381 agggcggcgg caggtttggg cgcaaacggc tttcagacgg catgccgtat cacgttcccc
     1441 ctcttgaaac cggcgttgcg gcgcggtctg actttggcgg cggcgacgtg tgtgggcgaa
     1501 tttgcggcaa ccttgttcct gtcgcgtccg gaatggcaga cgttgacgac tttgatttat
     1561 gcctatttgg ggcgtgcggg tgaggacaat tatgcgcggg caatggtgtt gacattgctg
     1621 ttgtcggcat ttgcggtgtg cattttcctg ctgttggaca acggcgaagg cggaaaacgg
     1681 acggaaacgt tataatgcga accctttttc agaggacggc aaaatgagcg agttgagcga
     1741 aatcctttcc tataatcaga agtttgtcga gtcgggagaa tatgaaaaat actttaccga
     1801 caaatacccc gaacgcgggc tggcggtttt gtcctgtatg gatgcgcgga ttatcgggct
     1861 gctgcccgac gcgttgggtt tgaaaaacgg cgatgccaag ctgattaaaa atgccggcgc
     1921 gctggttacg cacccgtggg gttcggtgat gcggagcctt ttggttgccg tgtttgaatt
     1981 gaaggtcaga gagattatgg tcatcgccca tcacgattgc ggtatgcagg ggctgaatgc
     2041 cgaagaattc ctcgggcgcg tccgggaaag ccggattccc gaagaccgta tcgaaaccct
     2101 gcgttatgca ggtgtcgacc tcgacggctg gctgaccggt ttcgacaacg tcgaagacag
     2161 cgtgcgccac acggtggacc ttatccgcaa ccatccgctg atgccgcgcc atatcgccgt
     2221 tcacggactg gtcatccatc ccgttaccgg caaactgacg ctggttgtgg acggcagtgt
     2281 ttcagacggc atggacttat cggaaggaat ggaaacatca tgaagaaaat cggactgttc
     2341 ggcggcactt tcgacccgat acacaacggg cattttcata tcgcccgtgc ctttgccgac
     2401 gaaatcgggt tggacgcggt tgttttcctg ccggcaggcg gcccgtatca caaagacgcc
     2461 gcctccgctt ccgccgccga ccgccttgcc atggtcgaac tggcgacggc agaagacgcg
     2521 cgttttgccg tcagcgactg cgacatcgtc cgagaaagtg caacctatac ttttgatacc
     2581 gtccaaatct tccgccggca gttcccgtcc gcgcaacttt ggtggctgat gggcagcgac
     2641 agcctgctga agctgcatac ttggaaaaaa tggcagttgc tcgtgcgcga aaccaatatc
     2701 gccgtcgcca tgcggcaggg cgacagcctg caccaaaccc cgcgcgaact gcacgcgtgg
     2761 ctgggcaatg cccttcagga cggcagcgtc cgcatcttgt cagccccgat gcataatgtg
     2821 tcgtcaacgg aaatccgccg caacctgagt gccgccggtg tttcagacgg catcccgcct
     2881 gtcgccgccc gttatatccg aaaacacggt ttgtatgaaa aataaagtca aatcagtaag
     2941 gaacggctat aatgccgtct gaaaacatcc cgttaggaaa acaatgaacg aacaagaact
     3001 gcaagacctg caaaaaatgg tcggggtcgc cgtcaatgcc ctcgaagaca tcaaagccaa
     3061 agacatttcc gttctcgaaa cgcaagacaa aacttcgctg tttgccagaa tgattatcgc
     3121 cagcggcgac agtacgcgcc aagtcaaagc actggccaac aacgttgccg tcgatttgaa
     3181 agaagccggt tttgaaatcc tcagtaccga aggagacagc ggcgaatgga cgttggttga
     3241 tgcaggagac ctcgtcgtcc acgtcatgct ccctgccgtg cgcgacttct acgacattga
     3301 caccatctgg ggcggcgaga aaccgagttt ccacgccgga atgcagaagc cgtggcacgc
     3361 tgcagactga ttcccgatgc cgtctgaata ttcatacggc atttcctgta agggggaaag
     3421 cattgaacat taccgtcttg gcagtcggca ccaaaatgcc gcgttgggtt gatgaggccg
     3481 tcgccgaata cgccaaacgc ttcggacgcg acgccgccta cgcactcaaa gaaatcaaac
     3541 ccgaaaaacg cggcgcgggc gtgaatgccg tccaaggtat ggcggcggaa gaaaaacgca
     3601 tccttgaagc cattccgcaa ggcgcgttcc tcgtcgttct tgacgaacgc ggcaaagcac
     3661 cgacctccgt cgagctggcg gaacacctca aaagctggcg gcaaaacggc gaacacgtct
     3721 gcttcgtcat cggcggcgcg gacggcatga ccgaccgcct caaacaacaa gcccgcatga
     3781 tgatgcgcct gtccagcctc accctgccgc acggcatggt gcgcgtcctt ctgaccgagc
     3841 agctctaccg ggccgtttcc atcctgcaca accatcctta tcatcgggaa taaaaaggct
     3901 tttgtctgta ccccctaatc ggttaaaatc gccccgttat ttctgaacac agcaaaggaa
     3961 tccgctatgg cacgaatggt attctgcgtc aagctcaaca aagaagccga aggcatgaaa
     4021 tttccgccgc tgcccaacga attgggcaaa cgcatttttg aaaacgtatc gcaagaagca
     4081 tgggcggcgt ggacgcgcca ccaaaccatg ctgattaacg aaaaccgctt aagccttgcc
     4141 gatccgcgcg cgcgcgaata cctggctcag cagatggagc agtatttctt cggcgacggc
     4201 gcggatgccg tgcagggata cgttccgcaa taacggtttt ccgttttgaa cacaggctgt
     4261 ccgaaactgc ttcagacggc ctttaaaata cgccggacaa ctttatcttc acgcaaccgg
     4321 acaaattttg acattgggca atgttaaaat cccccgttat ttccaaccgc gctttcagga
     4381 gcagatgata tgcaacacga cgtttacgac tacaccgcgc atacggtttc taaaaacacc
     4441 gtcctgcaga aaacctaccg cctgctcgga ttttcattca ttccggcagc cgcaggcgcg
     4501 gcacttgccg ccaatgccgg tttcaatttt tacgccgcct tcggttcgcg ctggatagga
     4561 tttgccgtcg tattggcgtt tttctacggt atgatccact tcatcgaaaa aaaccgttac
     4621 agcaataccg gcgttaccct gctgatggta ttcacattcg gtatgggcgt attgatcggc
     4681 cccgtgctgc aatacgcact ccatattgcc gacggtgcga aaatcgtcgg cattgccgcc
     4741 gcgatgaccg ccgccgtctt tttaacgatg tccgcattgg cacgccgaac ccggctcgat
     4801 atgaacgcgc tcggacgctt cctgaccgta ggtgcggtca ttctgatggt cgccgtggtt
     4861 gccaatctgt ttttgggtat tcccgcactc gccctgacca tttccgccgg ttttgtcttg
     4921 ttcagttcct taatgattat gtggcaggta cgcaccgtca tcgacggcgg cgaagacagt
     4981 tacatcagcg cggcactgac actgtttatc tcgctttaca acatcttcag cagcctgctc
     5041 aacatcctgc tgtccttaaa cggcgacgac tgatgtaggc gaaacaccat gcaaaccatg
     5101 ccgtctgaaa gcacttattt tcagacggca ttttgttttg ggcatacaat acggatgcaa
     5161 acacataaaa acacagccgg ctatgaagcc gggaggaatc aaatgttacc gaatacaccg
     5221 cgccgcgccg tttatgccgg cagtttcgat ccgcccacat tggggcatct gtggatgata
     5281 cggcaggcgc aatctatgtt tgacgaactc atcgtcgcca tcggcatcaa tcccgacaaa
     5341 cgcaacacct ataccgccgc tgaaaggcag gatatgttgt gcgccattac cgacaacttc
     5401 ccaaacgtca gaattgaagt gtttcaaaac cggtttttgg tgcattacgc ccgtgaggta
     5461 gatgcgggat tcatcgtgcg cggcatccgt tccacctccg attatgaata cgaacgctcc
     5521 atgcgccata tcaacagcga cctcgcaccc gaaatatcta ccgtattcct catgccgccg
     5581 cgcgaaatcg ccgaagtgtc gtccactatg atcaaaggac tggtcgggcc cgagggctgg
     5641 atggaaaccg tcaaaagata tgtgccgccg gccgtgtacc aaaaaatgat tgcagaacat
     5701 cacaacaaca atgcctgaca gaaagttcct gtcaggcata ttcgggttca aacccgtttg
     5761 aggtattggt tacaatttat tgtatattat caacggtttg aaaattatgc actttatcat
     5821 tggcgatata ctcaataaac cgttatcgcc acaaggctac atagcattca gacgtgcttc
     5881 taatttctcc aatcgtgcct ttaattgacg attatcttgc atcaactgtt ccataagcac
     5941 cttatcactt gtagccggaa caccttggct cgttccaaat ttccaagtca agcctacatt
     6001 catcatatgt cccgtcttat tgccgcctaa ggttccgcct acttttacca taaagcggtc
     6061 attgacgaaa tgggacaatc ctaatgccat agcttgacgt cctttgtatg tgccgaaacc
     6121 gaccataaac tgcgtaggat tgtttgcatc ataggataat ggagtcagag ccgatagggc
     6181 tgcactactc gctgccacac cacgcacttc gctgcgtact gaaaatagct cagccatatt
     6241 ttgtccttgc atcgctttca actggcgtac attgacggca tcaaaatcat tgacaccatc
     6301 atcaacacct acaatttttc tataggaaag ttctgtagat tgtggtgttt ctttataggc
     6361 aacagagacg acattattct cactagctac tgaaccataa cccggtgcca cagaattgct
     6421 cacaccatct tttatttcac tacgagatcc taaactcaca gaatgatttc cgtttgccct
     6481 tgcatatcgg ccaatgacaa gtgagttttc cttagtcgct tcagccttat tccccacagc
     6541 caccgcaaaa tctgcctctg ccttagattg gttaccgaga cctacgccgc cttgcccctt
     6601 tgccttgctt tcattaccca cggcggtact aaaatcaccg gcagcttctg attctacgcc
     6661 aagtgccatt ccattttgct tcgttgcttt tgcttgtttt cccaaagcga tggcgccacc
     6721 tttgccagcc tctgcttgat acccgacggc aatagcgtcc gcataatatg ccggtccatc
     6781 taacagtgtg ttcgttgttt tgccccctgc tttggcttct ttaccaatcg caataccacg
     6841 ttctgcattt acctttgcct cagaacctac cgcaaccctt tgtttagctt tgttctttac
     6901 cgttgcttta gaacctactg caaccccttg atctgcttcc ggttttactt gccttgaatc
     6961 gccaattaca acactgcctt ctgcactggc aacagaagca tagcctgcag cataggattt
     7021 atttccactg gcaacagaat tatagcccga cgcataggaa tactctccac tggcagtaga
     7081 actagatcct aatgctactg catatttctt ggggctgtac
//
LOCUS       DAWTCI010000093         7085 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWTCI010000093 DAWTCI010000000
VERSION     DAWTCI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.093"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(19..1785)
                     /locus_tag="WM481_001894"
     CDS             complement(19..1785)
                     /locus_tag="WM481_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFA9081149.1"
                     /translation="MIERLKAVENQAEAMGRGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKLISAVLAEAMRRNGKRLMPVRQAVEMLRANGKIE
                     AARIDGETGEVIPLSENTITRALREYKLHSDQLLQPDPVSRMKSEHPNHCWQIDPSLC
                     VLYFLPRQGKDTGLRVMKEEEFYKNKPKNVVKIENDRVWRYTGTDHASGTISVRYYFG
                     GETSANLCDFFIYMMQAKKDIGKDPFRTVPRMVMLDPGSANTSAAFKNLCKPLDVHVQ
                     INKPGNPRAKGQVEKANDIVETAFESGLRFTEVHDIDQLNALSERWMRYYNGTQKHSR
                     HGMTRYQAWNKIKPEQLILPPPAEYCRELAVSAPKEAKVSADLEIRFGGRVYSVKGIK
                     GILVGKKVLVGKNPWEANGARVATYDAEGNEVWVSVPEVVFDEMGFKADAAVIGAEYK
                     APADTDAQRHRKELDKLAMGAETLEAAAAKRKGKAVPFGGKIDPYKHQEDTLAASNTL
                     FMPKQGQQMDYNKMEVAEQVLSKVEIAKRLKPRVEADGGDWKQAVSVILKHYPEGVTE
                     GRLEEAFERTRTRCRLKLLKTG"
     gene            complement(1778..2651)
                     /locus_tag="WM481_001895"
                     /pseudo
     CDS             complement(1778..2651)
                     /locus_tag="WM481_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009426280.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2688..2972)
                     /locus_tag="WM481_001896"
     CDS             complement(2688..2972)
                     /locus_tag="WM481_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFA9081150.1"
                     /translation="MATSKKAQRLLRVFLAMDAHPVIGISNKELSDGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRKQQERLNARLQELENRIY"
     gene            complement(2959..3189)
                     /locus_tag="WM481_001897"
     CDS             complement(2959..3189)
                     /locus_tag="WM481_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFA9081151.1"
                     /translation="MEKPLNFKPIPYPQTRGSTAVWFKRNGVCKTHRAKHFNLERTAV
                     EHPLRGKLKGNFGKSHEAAVKFGLKEDADGDE"
     gene            3461..3841
                     /locus_tag="WM481_001898"
     CDS             3461..3841
                     /locus_tag="WM481_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9081152.1"
                     /translation="MEKTSLFGKRLKEERIKLGLNQAEAAEKCGFSREMWGKWERGEN
                     RPSSEKLFSFSKIGIDIDYVMHGRRGETAAMPSESLSAEEKELLALFRQLGSGSRKEL
                     ADYAAFKLVVEKKAQTALGKVSNG"
     gene            4075..5115
                     /locus_tag="WM481_001899"
     CDS             4075..5115
                     /locus_tag="WM481_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFA9081153.1"
                     /translation="MKKIITHRIYPKNENRERVEPKISTHLIDLPITARRTLETRLTK
                     ALGNKSHGIEMSVVNTAENSFFQIAAAIQLKEEAEFIEDSAQFAHMLTDAQLNTNAPG
                     GILLVLKGRVGDTGKPFLCVIKAEPQDGFRTKEEDDFITIEFLEELLLTDSARLFKIG
                     FLVAETVRPLEQIQSGNYRAFLYDHLMTQTETRPAASYFYQVFLGMSIAASSRKLTQD
                     FFEWTRNFIDNSDLSDDAKLDAHEALRVTLKSAEATISVNNFAQNHLPQEKRTTYTEF
                     MVEKDFPQNAVSKDIEYIKTRLRKRRSYGFSNGVVILTPPEHTQDYMEIAPTEDGEYT
                     VVLIKGQLQQQK"
     gene            5112..5486
                     /locus_tag="WM481_001900"
     CDS             5112..5486
                     /locus_tag="WM481_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081154.1"
                     /translation="MSDIDDFKSYLESHQAAFSAWGRFVAEEIQNQLSNVISPVPVAN
                     FLKIEAKPGVKEISSALAKIGRKNYTSPQTQMTDLVGVRFVALLAEHIQIVCEIIESS
                     SQWNAKVSKDFADEIQQNPKAI"
     gene            5483..6121
                     /locus_tag="WM481_001901"
     CDS             5483..6121
                     /locus_tag="WM481_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081155.1"
                     /translation="MTIQSKHYEIRPKQAFITPENVSIPADLCCEVQVRSLLQHAYAE
                     LVHDNIYKPDGNVPKQAEREVAKSMALMETTDDLFSRTLAILKEANQPQEELLPQLSQ
                     LYQKEIGLVPEVDKKTNMIFLETFQSSISQSSILSDIRSLLNEKKYIAKRIKENAEEM
                     YFFSQPAALLVYWLIEKVGADEVWKKWPLPAYNKNLKFICTDLDKQPSHELF"
     gene            6241..6528
                     /locus_tag="WM481_001902"
     CDS             6241..6528
                     /locus_tag="WM481_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081156.1"
                     /translation="MIEFVRAKKRLLWAFVLLLAWACGYRYAADKAEAEQTALIAAYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQSTDLAAALSELDKTRNTLQE"
     gene            6602..6823
                     /locus_tag="WM481_001903"
     CDS             6602..6823
                     /locus_tag="WM481_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081157.1"
                     /translation="MPSDTPIKTVAVAEIPPVPSGLLVEYERPERPAGGSPEQLLNHA
                     VRYGGYYRKLEIQIEGWQNWHTKGRLKHD"
     gene            6816..7025
                     /locus_tag="WM481_001904"
     CDS             6816..7025
                     /locus_tag="WM481_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFA9081158.1"
                     /translation="MTDFADRASEREAEFLVEALAKHQPPSENTAGFSHCEDCGDPIP
                     EAKRKAVRGCTRCVVCHEYSQLKTK"
BASE COUNT         1759 a         1791 c         1648 g         1887 t
ORIGIN      
        1 gctgttttcg gttgaaacct aaccggtttt caaaagtttc agacggcatc ttgtccgggt
       61 gcgttcaaac gcctcttcca gcctgccctc ggtcacacct tccgggtagt gtttgaggat
      121 gaccgacact gcctgtttcc agtcgccgcc gtctgcctcg acgcggggtt ttaagcgttt
      181 ggcgatttcg accttgctca atacctgctc ggcgacctcc atcttgttgt aatccatctg
      241 ttgcccctgt ttgggcataa agagcgtatt gcttgcggca agcgtatctt cttgatgttt
      301 gtacgggtcg attttgccgc cgaacgggac tgccttgcct ttgcgtttgg cggctgccgc
      361 ctccagcgtt tccgcaccca tcgccagctt gtccagctct ttgcgatgcc gttgcgcgtc
      421 tgtatcggcc ggggctttgt attccgcccc gatgaccgcg gcatcagcct tgaagcccat
      481 ctcgtcaaaa actacttcgg gtacggatac ccaaacctcg ttaccctccg cgtcataagt
      541 ggcgacccgc gccccgtttg cctcccaagg attcttaccg accaaaactt tcttaccgac
      601 caaaatcccc ttgatgcctt ttacgctata tacccgtccg ccaaagcgga tttccaaatc
      661 cgccgagact ttcgcctctt tcggcgcgct gacggcaagc tctcggcaat attcggcagg
      721 cggcggcagg atgagctgct cgggtttgat tttgttccac gcctgatagc gggtcatgcc
      781 gtggcggctg tgtttttgcg taccgttgta gtaacgcatc cagcgttccg ataaagcatt
      841 gagctggtcg atgtcgtgta cctcggtaaa gcgcaacccg ctctcaaatg ccgtttccac
      901 aatatcgttg gctttttcca cttgcccttt ggcacgcgga ttgcccggct tgttgatttg
      961 cacatgcaca tccaagggct tgcacaaatt tttaaacgcc gccgaagtat tcgcgctgcc
     1021 cgggtcaagc atgaccatgc gcggtacggt ccggaacggg tctttgccga tgtctttttt
     1081 cgcctgcatc atgtagatga aaaaatcaca gaggttcgcg ctggtttcgc cgccgaagta
     1141 ataacgcacc gaaatcgtgc cggaggcatg gtctgtcccc gtgtaccgcc aaacgcggtc
     1201 gttttcgatt ttgacgacgt ttttcggctt gtttttataa aactcctctt ccttcatcac
     1261 ccgcagcccc gtatccttgc cctgacgcgg caggaaatac aaaacgcaca aactcgggtc
     1321 gatttgccaa caatggttcg ggtgttccga tttcatgcgg ctgacggggt cgggctgcaa
     1381 aagctggtcg gaatgcagct tgtattcccg taaagcccgg gtaatggtgt tttcagaaag
     1441 ggggatgact tccccggttt ccccgtcaat ccgcgccgcc tcgattttcc cgttggcgcg
     1501 cagcatttcc accgcctgcc gcaccggcat caaccgcttg ccgttgcgcc tcatcgcctc
     1561 cgccaaaacc gccgaaatca atttggcttc ttccggttta agctccgtct tgcccgcatc
     1621 gctgcgccgt ttgcgcgtcg gcttgacgct gaccgcctcc agcttgcggt atagcgtggc
     1681 aaggctgatg cccaattcct gcgcctgctg cttaagatat gcagagcgtg cgccgcgccc
     1741 cattgcttcc gcctgatttt cgactgcttt aagacgctca atcattgccg gattcatcgc
     1801 cttctcccgt ttcaccgccc aaccattccg gcacattgtc tgccggtgct tcggtcggta
     1861 gggcatagct ttcgcgcagt tgctcgcagt ccaaaataat ttgattgagc gtgccgacca
     1921 tcttcgcgcg gtggtcaaat ccatgcgcct cgccgtgcgc cgccatctgc tcgaacatat
     1981 cgcgcaatcg gctgatttgc gagcggatac cgacttccaa gctgccaagc tgcatcgtca
     2041 gctcgatgcc cacatccgca ggtttaggct ctttgacacc cgtctgcttt ttcgacagct
     2101 tttccgccag ctcatcgacc tttttatttt tatcggcaat caccttatct ttcgcttccg
     2161 ccgtttcacg gctttcgcgc agggcgacgc gcagctcctt aatagtcata cggtcgacat
     2221 cgtcaaaagt gttgccgttg acctcaccgc cttcggcaag ctccaacaaa gtgtcgtcgt
     2281 cttcgaccag cagctcgagc agtttggatt tgcccaacgt catcagctta ggctgcgctt
     2341 gtttcatttt cgggtcgata aaacggaggg tggcattcat cagtctttgg gattcccgcc
     2401 gtccgaggcc aactcttttt cagcgatttc ggcaaaccgc ccatgcggcg tatgctcttt
     2461 gatgatgatc agcgcgcgtc ccggctcaaa catcccttcc atcgtttggc gtccgcaaat
     2521 cgtccgcgct caatccatac cgtctcgttg taggcctcgc cgcctgaaaa cttgtccata
     2581 acctccatac tgtggatagc caattcgttt gccgttacgc cgaccgtatg tcctaaaatt
     2641 tctgttgcca tttcttttgc tcctcaaatg cgacgtcgtc gtcgcattta gtaaattcgg
     2701 ttttccaatt cttgcaagcg cgcattcagc cgctcttgct gttttctgaa ccgctctgcg
     2761 atttgtaggg ttttgatgcc gtaggcgtag ttgccgtttt caagcttgat gaccaatccc
     2821 gatgcaacca aatcatcaat atccctgctg acttgtgatg gcgtcagccc caggccgtct
     2881 gaaagctcct tattgctgat gccgattacc ggatgcgcgt ccatcgccaa aaacacccgc
     2941 aacagccgct gcgccttttt actcgtcgcc atccgcatcc tccttcagcc caaattttac
     3001 cgccgcttcg tggcttttgc cgaaattgcc tttcagcttg ccgcgcaagg ggtgttccac
     3061 cgcggtgcgc tccaaattga aatgtttcgc ccggtgcgtc ttgcacacgc cgttgcgctt
     3121 aaaccacacg gccgtgctgc cgcgcgtttg cggataggga ataggcttga aatttaaagg
     3181 tttttccata ttatttatgc ctttcgtgtg gtataatgtg tttagtttta ggcctctttg
     3241 cacgataaac atccggtctg ttgtgcagca gcagggtctt ggcttgtttt aaaataaaca
     3301 agctttttcc cgtagccggg tgtatgtgat ccatcatgag ctgtcgcgca tgagccttca
     3361 aagtgttcat tttttcgtcc tttttcgtga tgatttaggg tgtttcatgt cttgatgtgt
     3421 gaattatgag aaaaatattt ctcttttcaa ggaaatttaa gtggaaaaaa cttctctttt
     3481 tggtaagcga ttgaaagaag aaagaattaa attaggcttg aaccaagcag aagctgctga
     3541 aaaatgtggt ttttctcgtg agatgtgggg aaagtgggag cgtggtgaaa atcgcccatc
     3601 aagtgaaaaa ttattctctt tttctaaaat cggcatcgat attgattacg tcatgcacgg
     3661 cagacgcggc gaaacagccg ccatgccgtc tgaatccttg agtgctgaag aaaaagaact
     3721 gcttgccctg ttccgtcagc tcggcagcgg cagccgcaaa gagcttgctg actacgccgc
     3781 ctttaagctg gtggtagaga aaaaagccca aactgcgctt ggtaaagtga gcaacggata
     3841 aaaaatgccg tctgaagatt tcagacggca tttgggacac tctgacagtc gggctgctcc
     3901 ggtgttctga aacagatatt aagcaatagg agcggccgtg cattttaaag cgtattaaaa
     3961 accaccccga tattcaggtc atcggccaag tggttcagat ttcaaaagac cttaattaac
     4021 ttacaatagg aaaaatgatg acagaaacga attactcttt tgaagggctg gtaattgaaa
     4081 aaaattatta cccatcgtat ttatccgaaa aatgaaaata gagagcgtgt agaacctaaa
     4141 atcagtacac atttgattga tttgcctatt acggcaagaa gaacattgga aaccaggctg
     4201 accaaagctt tgggtaataa atctcatggc attgaaatgt ctgttgtcaa tacggctgaa
     4261 aatagttttt ttcagatagc ggcagccata caactcaaag aggaagcaga gtttattgag
     4321 gattccgcac aatttgccca catgttgacc gatgcacaac tgaatacaaa tgcaccgggt
     4381 ggtattttgt tggttttaaa aggtagggtt ggagataccg gtaagccgtt tttatgtgta
     4441 attaaggctg aacctcaaga tgggttccga accaaagaag aggatgactt tatcacgatt
     4501 gaattcttag aagaattatt actgaccgat tcagcaagat tattcaagat aggttttttg
     4561 gtggctgaaa cagtaaggcc gctagagcaa atacaatctg ggaattatcg agcttttttg
     4621 tatgaccatc tgatgacaca aacggaaact agaccggcag cttcctattt ctatcaagta
     4681 ttcttgggta tgagtatagc tgcttcttcc cgtaaattga cgcaggattt ttttgagtgg
     4741 acacgcaatt ttatcgataa ctctgattta agtgatgatg caaaattaga tgcgcatgaa
     4801 gcattgcgcg ttacattgaa aagtgcggaa gcaaccatta gtgtaaataa ttttgcccaa
     4861 aatcatttac ctcaagaaaa acgaacaact tatacagaat ttatggtgga aaaggatttt
     4921 cctcaaaatg ccgtaagtaa agatattgaa tatattaaaa ctcgtttacg caaacggagg
     4981 tcttacggat ttagtaacgg tgtagttatc ttgactcctc ccgagcatac tcaggactat
     5041 atggaaattg cgccaacgga agatggggaa tatactgttg tcctaattaa aggacagtta
     5101 caacaacaaa aatgagtgat attgatgatt tcaaaagcta tttagaaagc caccaagcgg
     5161 cattttccgc ttggggtaga tttgtggctg aagaaattca aaatcaatta tccaatgtca
     5221 tttctcctgt tccggttgcc aattttctaa aaattgaagc aaagcctgga gtcaaagaga
     5281 tttcttcagc tctagccaag attggccgaa aaaattacac ttcacctcaa actcaaatga
     5341 ctgacttggt cggcgtacgt tttgtggcat tgcttgcgga acatattcaa atagtttgtg
     5401 aaatcattga atcctcatct cagtggaatg ctaaagtttc gaaagatttt gcagatgaaa
     5461 ttcagcaaaa tcccaaagca atttgactat acaatccaaa cattacgaga ttcgtcctaa
     5521 acaagcattc attacacccg aaaatgtcag catccctgct gatttgtgct gtgaagttca
     5581 ggtgcgttct ttactccaac atgcttatgc agagctggtt catgacaata tatacaagcc
     5641 tgacggcaat gttccaaagc aagcagagcg cgaagtcgcg aagagcatgg ctttgatgga
     5701 aactacagat gacttattta gtcgtactct ggcaattttg aaagaagcaa atcaaccaca
     5761 agaagagctt cttccccaat tatcacagct ttatcaaaag gaaatagggc ttgttcctga
     5821 agttgataaa aaaaccaata tgatttttct agaaacattt caatccagca tttcccaatc
     5881 aagtattctt tctgacatcc gttcactttt gaatgagaag aaatatattg ccaagcggat
     5941 aaaagaaaat gctgaagaaa tgtatttttt cagtcagcca gcagctttgt tggtctattg
     6001 gctgattgag aaagttggtg cagatgaagt atggaaaaaa tggcctttgc ctgcatacaa
     6061 caaaaacctg aaattcattt gtactgattt ggataagcaa ccatcccatg aactctttta
     6121 acccccttta aaagcccatt cagacggcct ttcctaatta tgccttaatc aaacgcggca
     6181 tagcggcagt tccgcagctg gcggaaatca aaaaatccgc aaatcaggaa gggggcggca
     6241 atgattgaat ttgtccgagc caaaaaacgg ctgctttggg catttgtact tttgcttgcg
     6301 tgggcgtgcg gctaccggta cgctgccgac aaggccgaag cggaacaaac cgccctgatt
     6361 gccgcctatc ggcattcttc tatggttgcg gcggaacaat acgccttgca gcttaaaaaa
     6421 gcgcaggacg aaaggcagcg gtggtacgac ttttcccaaa aacaaagcac ggatttggcg
     6481 gccgccctga gcgaattgga caaaacgcgc aacaccttac aggagtaaac ccgtgaagcg
     6541 gcagacaaag acggcaaccg ttttaacggc cttggcacga acagcctgca cctctacaac
     6601 cgtgccttcg gataccccga ttaaaaccgt tgccgtcgcg gaaattccgc ccgttccttc
     6661 cggactgctg gtcgaatacg aacgccccga gcgtccggcc ggcggctctc ccgaacaact
     6721 tttaaaccac gccgtccgct atggcggata ctaccgaaag ctcgaaatcc aaattgaggg
     6781 ctggcagaac tggcacacga aaggccgtct gaaacatgac tgattttgcc gaccgcgcat
     6841 ccgaacgcga agccgaattt ctggtggaag ccttggcgaa acaccaaccg ccgtctgaaa
     6901 acaccgccgg cttcagccat tgcgaagact gcggcgaccc gataccggaa gcaaagcgaa
     6961 aagccgtccg aggctgtacg cgctgtgttg tctgccacga atatagtcaa ttaaaaacaa
     7021 aatagtacaa cactcgacgt tgaaggtcta accatggcat actctgcgga cttaagaaac
     7081 aaagc
//
LOCUS       DAWTCI010000094         7081 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWTCI010000094 DAWTCI010000000
VERSION     DAWTCI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.094"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(584..943)
                     /locus_tag="WM481_001905"
     CDS             complement(584..943)
                     /locus_tag="WM481_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081159.1"
                     /translation="MIHKPRYIKIVDENGDFTRVLRLHKFPDTSKVFYFEPMFWLKDG
                     RLARKDSLFEVDYIYGADGCGFLPSNLTEFRKYCRKKHQKFKDDEVLVNRYAVDFLGA
                     KEPPYDDRHVTSVKYFV"
     gene            complement(1064..2136)
                     /locus_tag="WM481_001906"
                     /pseudo
     CDS             complement(1064..2136)
                     /locus_tag="WM481_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2146..2436)
                     /locus_tag="WM481_001907"
     CDS             complement(2146..2436)
                     /locus_tag="WM481_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9081160.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLAALEKFK
                     GYTANAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(2415..3377)
                     /locus_tag="WM481_001908"
                     /pseudo
     CDS             complement(2415..3377)
                     /locus_tag="WM481_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
     gene            3563..3847
                     /locus_tag="WM481_001909"
     CDS             3563..3847
                     /locus_tag="WM481_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HFA9081161.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYAGVDRLAAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            3848..5035
                     /locus_tag="WM481_001910"
     CDS             3848..5035
                     /locus_tag="WM481_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HFA9081162.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGVRRKVFTNI
                     KGLKIPHTHIETDAKKLPKSTDEQLSAHDMYEWIKKPENVGAIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVKRHYHIAANKMGLRTLLEW
                     KVCADDPVKMASSAFSSIYTLDKKVYDLYESAEIHTVNKVKRSKWFYALPVIILLIPL
                     FVGLSYKMLGSYGKKQEEPAAQESAATEQQAVLPDKTEGESVNNGNLTADMFVPTLPE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKPQQNLMYDNWEERGKPFEGIGGGVVG
                     SAN"
     gene            5250..6195
                     /locus_tag="WM481_001911"
                     /pseudo
     CDS             5250..6195
                     /locus_tag="WM481_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
     gene            6498..7037
                     /locus_tag="WM481_001912"
     CDS             6498..7037
                     /locus_tag="WM481_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFA9081163.1"
                     /translation="MTELPWIAEARRHIGLKEIPGAKHNPTIVQWLKETGGFPGAAKS
                     WYFEDETPWCGLFVGHCLGKAGRAVIRDWYRAKAWSMSGLTKLEAPAYGCIAVKPRRG
                     GGHVFFVVGKDAEGRILGLGGNQGNMVSIIPFDPADIDGYFWPSKLIGGKPVPSSPAE
                     GRYRLSDVAATAKQGAGEA"
BASE COUNT         1991 a         1641 c         1674 g         1775 t
ORIGIN      
        1 ctccgttttg ccgaccatgg cggatgcgtc aaatgtcagc ttggatatat ccaatcctac
       61 ggcgttacgc atgggattac ccttatatat tcagaatctg tgttctttga tactactcaa
      121 tttcacaaac aagaaaaccg cccgcctatt ctcgtcatca aactttaagt ttgtggtttg
      181 ttcaggccgg acggtttcgg caaagggtag ctattccttt gccgtgtctg attttatttg
      241 ggttgcaggt tttggtaaag attcctgttg cgacccgaat gtctgatttt ttttaggcgt
      301 atctcagtcc ggaatcactc cgttagtggg tttgcggtat tgaaaaacag ttcataaaaa
      361 aggaaaaggg ggtattcgta aagattgggt aacgcaccca atctttacaa agcttccccc
      421 ttttcctttt ttccgcccta ttttcctgca cgtacaaccc ccgaacgaag cgattccgga
      481 ctgagatacg cccaaaaaaa aacagccatt ctagcagtta acccccttcg ctccgcccaa
      541 gccatcctga ggggtagtgg ctgaatttgt gattttggtt ttatcaaaca aaatatttga
      601 ctgaagtcac atggcggtcg tcatatgggg gttccttcgc acccaaaaaa tcgaccgcgt
      661 aacggtttac taaaacttca tcgtccttaa acttttggtg ctttttccgg caatattttc
      721 tgaactccgt taaatttgac ggcaagaacc cgcaaccgtc tgcaccgtaa atgtaatcaa
      781 cttcaaacaa actgtctttc cgggcaagcc gaccatcttt aagccagaac ataggctcga
      841 aataaaaaac tttcgacgtg tccgggaact tatggagacg gagaacacgt gtgaaatccc
      901 cgttttcatc tacaattttg atatatcttg gtttgtgaat catgacatcc tcagatttag
      961 tattcagaat atgattttaa aaagaacttt ctgctttacg actccgccgc cgattccttc
     1021 aaacggtttt ccgcgctctt cagttgtcgt acattaaatt ttattaggac tttccgccca
     1081 ttacgagaac ttggggcttg tccgctttcg cggactgtgc cgcctgttcc gtcctttgcc
     1141 gttcgtcctt gtaaggattg aaaggcaacc cgtttttcac atattcttta cacattatct
     1201 ttgttatttc tttcaagggt gttccttgat ttgaatagca tgtgcaatct gattttccgc
     1261 cgtctatgca tccggcgatt tgctcaaagg tttttacttg tcggactgtg ttataaatag
     1321 gcttgctttc gggcttttcg ggcaaagtcg gcacaaagtc ttcaggtttc agattgtcgg
     1381 aatgctcaaa aggcgctgtt tctgatgatg ccgtctgctc cgtcatcgtc tgcacaacgc
     1441 tttctttttg cgcttcctgc tcaatccggc tgtctgtggc tttgctgtaa acttgaaaca
     1501 tgccgtaact tttccagcct acaaacccta caaccgcaat caacgcccaa accgcccaag
     1561 gcactttttt cttgaacttt tggtgccggc ttgatgattt atagtatttg aaggcttctt
     1621 taggcggttt ccaatttgcg gcttctacgc cgcttacgcc cgcgggattg tccaacgagg
     1681 ttacgcattt ataccaataa tactgtttca tgccgattgc cttgcgttca aggtgtacat
     1741 gctttgaaac aaggttgcgg acgaatatat caagttggct cgggtgccgc gtcatcaaaa
     1801 tgacggtatg cccgtgatgg cggagttctg tcagttcctg aatatagggc ggaacgggac
     1861 ggcctgccgc gcgtaccggg taagtgtagt gcgcttcgcc aacaatcagc accgcgcctt
     1921 ccggtatgac atcacgaagc ggggcggaca tgatttgccc ttccgccagt tcgcgggcat
     1981 tgaattttcg tttgtccaat ccgtcgatat ggcagaaata aagcggccgg tctgcctccg
     2041 tgccgtcttc caatttcatt ttgaacaatc cgtcttcgtt gttcaaaatc atagggtttt
     2101 gcctgccccc atgtttcccg taaacagata aatcatgctt ctacctcatc ccggaaagac
     2161 aaacgtcagt tttttgaatg cgtgcatacc aatgaagaac gagaatgcgc cgaacaggta
     2221 gcccaacccc tgaccgaatc ccgaaattaa aagaaggttc aatatgtcgg aaggcatgga
     2281 attgatcgca tttgccgtgt agcctttgaa cttttccagt gcggcgagat acccggcata
     2341 ggttacgaat gtcagacctg ttgcaaggat gattctgaca atcagcattt tcagaagtat
     2401 gcctaaaagt ggaatcaggc cggcaagtaa tggcatttat tcccccccaa cgaaccgaaa
     2461 acgacaaaag ccgacataat gataaaggcg agcagtacgg caaaacggat tttttcggca
     2521 aacacgcaca acggttcata gcttgcccga tattgcctgc cgaaaacatg aaaggttttc
     2581 ggctgcggac atacgccgtt agacggtaaa aagttatgtg aagaccatgt tttatcgtct
     2641 gtaacctgcg gtatgcttat atcgtgaaac atgcggtccg aaggtttgcc catctcctga
     2701 caggctagga tttccggaaa ataatcgcac gaaagcccgc cgtcttcgcc ttgtttcctt
     2761 tctttgcgat gcctgccgtt tgggcggtcc ggaacggccg gggaatcggg gcttgttccg
     2821 ggctgtccgt ccgtatcggg atttgcatcg ggattcaaat cgggatcggg attgggacgc
     2881 gtgccggggt tctcatcggg gtccgggttg tttgcggggt tttccgcggg cgatacttcg
     2941 ggcggcggct gtgcgtgagg tgcttccgcg cttgcgggcg tgaggtcggg acgcgggatt
     3001 acttgaacat ccgccgtggt gttgccttgc gcgtccctgc cgaatgttgc ggcaacctga
     3061 acgggattcc cgttcctgtc cgtgacgggc cccatattca cttttgttcc gggtgcgact
     3121 tctacttttt cggaataacc gggatatccg gttgccttta tgtatttgtc gggatcggca
     3181 tcgactttca acgataaaat ctcttccggc tttttggcat ccatttcttc tttgtatttc
     3241 ggattgcggc caagggaaaa agaagcccca gctctgaaat catcaccttt attgaccgca
     3301 caacctccgc cgttccaatc aaatgtgcaa cgatttaaaa caaaattatt ccaatccaat
     3361 taagcaactt tagccaaagg caggcgaagc atcgcacttt gcgggcgaag ccgcaaacag
     3421 ccgagaagcg cgggggggga ttggcgataa gcgcgagggg gtgtccccac agcgctgccg
     3481 cgccgcgaat gcggagcaat ctttcagatt aagaaacatt tgtttaatga ggcaaacgtg
     3541 ccttttaaga aagggagagc aaatgaaatt gttggccgca ttgattccgc tcttgatgag
     3601 cgtcgcaggc cgtatattga ctgcattagg cttgatggct gttacctatg cgggtgtaga
     3661 tagattggca gcccattttc aacaggcgat aacccatagc ataacgggcg cgcctcaagc
     3721 aatgttacag cttttctata taagcggcgg tggtactgtt ctaaacattc ttttcggcgc
     3781 aatcgccttt attctgtcct ttaaacaaat gacaaaactc gcaacctcaa tcgggaagaa
     3841 aaaataaatg gcagaaatct gtttgataac cggcacgccc ggttcaggga aaacattaaa
     3901 aatggtttcc atgatggcaa acgatgaaat gtttaagcca gatgaaaacg gcgtacgccg
     3961 taaagtattt acgaacatca aaggtttgaa gataccgcac acccacatag aaacagacgc
     4021 aaagaagctg ccgaaatcaa ccgatgaaca gctttcggcg catgatatgt atgaatggat
     4081 caagaagcct gaaaacgtcg gcgcaatcgt tattgtcgat gaggcgcaag acgtatggcc
     4141 cgcacgctcc gcaggttcga aaatccccga aaacgtccaa tggctgaaca cacacaggca
     4201 tcagggcata gatatatttg tattgacaca aggtcctaaa ctcttagatc agaacttgcg
     4261 aacattggtt aaaagacatt accacattgc ggccaacaaa atgggtttgc gtaccctgct
     4321 tgaatggaaa gtatgcgcgg atgacccggt aaaaatggca tcaagtgcat tttccagtat
     4381 ctacacactg gataaaaaag tttatgactt gtacgaatcc gcagaaattc acacggtaaa
     4441 caaagtcaag cgttcaaaat ggttttatgc attgcccgtc atcatattat tgattccgct
     4501 atttgtcggt ttgtcttaca aaatgttggg cagttacgga aaaaaacagg aagaacccgc
     4561 agcacaagaa tcggcggcaa cagaacagca ggcagtactt ccggataaaa cagaaggaga
     4621 atcggtgaat aacggaaacc ttacggcaga tatgtttgtt ccgacattgc ccgaaaaacc
     4681 cgaaagcaag ccgatttata acggtgtaag gcaggtaagg acctttgaat atatagcagg
     4741 ctgtatagaa ggcggaagaa ccggatgcac ctgctattcg catcaaggga cggcattgaa
     4801 agaagtgacg gagttgatgt gcaaggacta tgtaaaaaac ggcttgccgt ttaacccata
     4861 caaagaagaa agccaagggc aggaagttca gcaaagcgcg cagcaacatt cggacagggc
     4921 gcaagttgcc accttgggcg gaaaaccgca gcagaaccta atgtacgaca attgggaaga
     4981 acgcgggaaa ccgtttgaag gaatcggcgg gggcgtggtc ggatcggcaa actgaagaaa
     5041 acggcaacag agaaaaaaga cccgtaaacc gtttgaatat agacggctta cgggtctttg
     5101 tttcgcgcaa agcaagggct aaggcagtca ggcagcaaat cccgcaatgt attaaaacag
     5161 acgcgtagaa atgccggctg cctttatcca tcctcaaaat tgaatatcat cctagccgta
     5221 tcaaggctgt ataaataagg aaaataccaa tgaatataat cgggtcggac atctcaaagg
     5281 acaccataga cgcaacattg cataaaacaa acggaagtat ccattacatt aaatttaaga
     5341 ataatgatga tggattaaaa cagtttagat tgtggataaa gggaaacaga atcagaaaag
     5401 tctatatcgg catggaggca acaggcatct attacgaaaa ggcagcagat atgctttctt
     5461 cctactatac cgtttacgtt atcaatccct taaaaatcaa ggactacgga aaaagcaggt
     5521 ttaaccgcac caaaaccgac aaagcagatt caaacctgat agcagattac ataaaaaggc
     5581 atcaagatac attgataccg tatcagatac ccaaaaacaa agcactgcaa aaactgatta
     5641 atcttaaaaa ccaattacag caacagcaga agcaaattaa aaaccgtctt catagcactg
     5701 aagaagactt cataaggaac atacatcaag acttgataga taccatacag gacaagatgg
     5761 aacaggtaaa aaatagccat atccgaacaa atcaaaaaac aaacggacaa taaccattac
     5821 cgcaatcttc aaaccatccc gagcataggc aaagacaccg catcagttct ttatgcgcaa
     5881 ctgacagaaa aacattttaa aaccgcaaac caatttgtat cctatgccgg attaagtccc
     5941 gccatcatac aatcagggac aagcgtaaga ggtcggggca gattgagccg atacggaaac
     6001 agacgattaa aaagtacgct gtatatgccc gccctttgtg cttaccgttt taacgcattt
     6061 ccgaaattaa taaataatct gaaaaaagcg ggtaagccaa agatggtaat tatcgttgcc
     6121 atcatgcgca aactggcgaa gcccgcctat tacattgtta aaaccgggca gccttacgat
     6181 gcggaaagac actgattgaa tcaataaaat tcaataaaat taaacagtta tgcaaatata
     6241 tctttgtaac cgtgcatttg catatcgtaa ataaacgtaa ataaaaataa caatataaat
     6301 cagcatgttg caactttgtt ttttattttg tgttgacggg caacatatca tctgcgcgga
     6361 aagttaccgt atcggcaaac gcgactgaaa aagacagcgt gttcggcgat gcggtgcaca
     6421 ttgtgattgc gacttaaaac atagaacgag ttgccaaccc aaagtttaca tctcgtcttt
     6481 taccgaaagg attaaaaatg acagaattac cgtggatagc cgaagcgaga aggcacatcg
     6541 gtttgaaaga aattcccggc gcgaaacaca atccgacgat tgtgcaatgg ctcaaagaga
     6601 cgggcggctt ccccggcgcg gcaaagtctt ggtattttga agacgaaacg ccgtggtgcg
     6661 gcctgtttgt cggacactgc ctgggcaaag cgggacgcgc ggtcatcagg gactggtatc
     6721 gcgccaaagc ctggtcaatg tcgggtttga cgaaactcga agcccccgca tacggctgca
     6781 tcgcggtcaa accgcgccgg ggcggcggac acgtgttctt cgttgtcggc aaagacgcgg
     6841 aaggcagaat cttgggcttg ggcggcaatc agggcaatat ggtatccatc atcccgtttg
     6901 accctgcgga cattgacggc tacttctggc cgtccaagct gattggcggc aaacctgtgc
     6961 cttcgtcccc cgccgaaggg cgttaccggt tgtcggacgt tgccgccacg gcgaaacagg
     7021 gcgcgggcga ggcgtaaatg attggggctt tgctgaaaaa ttggaagccg ctgcttattt
     7081 t
//
LOCUS       DAWTCI010000095         6983 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWTCI010000095 DAWTCI010000000
VERSION     DAWTCI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.095"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            183..629
                     /gene="yciA"
                     /locus_tag="WM481_001913"
     CDS             183..629
                     /gene="yciA"
                     /locus_tag="WM481_001913"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFA9081164.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLATP"
     gene            complement(680..1501)
                     /locus_tag="WM481_001914"
     CDS             complement(680..1501)
                     /locus_tag="WM481_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFA9081165.1"
                     /translation="MAVLITGASAGFGEAMCRTFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSCRESVENVLNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERVAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1620..2078
                     /locus_tag="WM481_001915"
     CDS             1620..2078
                     /locus_tag="WM481_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFA9081166.1"
                     /translation="MKTQISLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2221..3357)
                     /gene="lst"
                     /locus_tag="WM481_001916"
     CDS             complement(2221..3357)
                     /gene="lst"
                     /locus_tag="WM481_001916"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFA9081167.1"
                     /translation="MGLKKVCLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGKPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLNKSFNFIPTMAELKVKSMLLPKVRRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTNNLIRESSYLGDEFYVNGAIKRNFARMMVGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTALNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFRQADIPILAFDDKNQSHGKSK"
     gene            complement(3348..3608)
                     /locus_tag="WM481_001917"
     CDS             complement(3348..3608)
                     /locus_tag="WM481_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081168.1"
                     /translation="MAALFALCSILACLNAAAGKEGRNGFPAFGGGVGGVVPDVRRHI
                     FGKIDFNIRQLSEYLLKFRIFRTGTLGAYVQFVGMEFLGIWG"
     gene            3687..5912
                     /locus_tag="WM481_001918"
     CDS             3687..5912
                     /locus_tag="WM481_001918"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFA9081169.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPGANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDAAVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLIANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGNVLMQHD
                     VEAGGIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFAPLAAALTADEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQVLNA
                     L"
     gene            6209..6511
                     /locus_tag="WM481_001919"
     CDS             6209..6511
                     /locus_tag="WM481_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081170.1"
                     /translation="MKTVPTILTGILLATALPASAHGMHKSKPLAMDELPPICQQYFK
                     RTETCYNKAGNKADFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRNLNCE"
BASE COUNT         1884 a         2040 c         1547 g         1512 t
ORIGIN      
        1 aggatttggc gggattggtg ggctgaagcc cgccctacgc atccgcccta caatactcct
       61 tgcgaatctg tcccgcgccc gaccttgtat gtctcaaatg ggttaaacta cgcacttccc
      121 gacttcccgt ttcgggcagg ggtgcaggtc cgatttatat ttcaataaaa caaggaaact
      181 ttatgcagca cgaagaaggc aaccgccaac gccctcaagg cgaactgctc ctgcgtaccg
      241 tcgctatgcc gcgcgatacc aatcccaacc aagacatttt cggcggctgg attatgtcgc
      301 aaatggattt gggcggcggc atattggcgg cggaaatcgc gcgggggcgc atcgttaccg
      361 tcgccgttca ggaaatgaac ttcatccgcc cggtcaaggt cggcaacgtc gtctgctgct
      421 acgggcattg cgtccgcgtg ggcaatactt ccctccagct taaagtcgaa gtctgggtga
      481 aaactttgat gaacgattgc gttaccgaag accgccatct cgtaaccgag gcggtgttca
      541 cttatgtcgc catcgatgcg gaaggcaatc cgcgcccgat tccgaaagaa ggcaacccca
      601 aattgtcagg cttattggcc accccgtaag aatacccgta aaaatgccgt ctgaaactgt
      661 cttcagacgg cattccgtat catttgaacc aagacctgat gcgggaaaac agcgacatac
      721 tctgtttttc aaagtcttcc cgcacttcgg gcacggctgt ttttatgacc ttcattcctg
      781 caaaagtctg cgccacaggc ataatttcaa tcgtgttcac attcatatgc gccggccggc
      841 ggtacagcca caatgcggtt tccgcaatat cttcggggcg gataaattcc acaccctcat
      901 acacgcccgc caccctctcg ttatcgcctt tgaagcgcac gttggaaaac tccgtattgc
      961 cgcacaaacc cggctcgata ttggtaacgc ggatgttctt atccgccagc tctgcgcgca
     1021 aattcaggct aaactggcgc acaaacgcct tggtcgcccc gtaaacgttg ctgccggcat
     1081 aagcataatt gcctgcaatc gaacccaaat tcatcacata accgccgccg cgttccacca
     1141 tttgcggcaa aatcttgcgc gtcaggaacg tcaaacccaa aacattggtt tgaatcattg
     1201 tttcccaatc ttcaaaatcc gccttgtccg ccgtgtccaa acccaaagcc agcccggcat
     1261 tgttgatgag gcagtcgatg tcggaaaatt catcggggat gccgttcaag acgttttcca
     1321 ccgactcgcg gcacgacacg tccatttcca aagggtaaaa caaagcaccc aattcatccg
     1381 ccaaagcctg aagcctgtcc gcacggcgcg ccgcaccgat aacgcggtat cccgccccga
     1441 caaacgtgcg gcacatcgct tcgccgaagc ctgccgaagc accggtaatt aaaacagcca
     1501 ttgtttttcc tttctttctc gtttcccaaa cctgtttcag gtatcataac acccttcaga
     1561 cggcatcgcg ccggctgaaa acgacaacaa ccgtaccacc acgacaggac acattcaaca
     1621 tgaaaaccca aatcagcctt gccgcaaccg ccctcgccct attcctttcc gcctgcggaa
     1681 acggcggcgc gcccgcacag cccaaaggcg aaatttccga aaaccgcacc gccgcgttca
     1741 aatctatgat gcccgacttc tcgcgtatgg gcaaaatggt caaaggcgaa gagccttacg
     1801 atgtcgaaaa attcaaacag gcggcggcgg cgtttgccga aagcagcaaa aaaccgttca
     1861 cactttttga gtccgatccg caaggcaacg gccgcgccct gcctgccgtt tggtcggatg
     1921 gtgcaaaatt tgaagccgaa aaaacaaaat tcgccgccgc cgtcgagaaa ctcaacgccg
     1981 ccgcccaaac cggcaaactg gacgaaatca aagccgccta cggggaaacc ggcgcaagct
     2041 gcaaatcctg ccacgacagt ttccgcgcac cggaataaca tccgaaacaa aacaatgccg
     2101 tctgaagcct tcagacggca ttttctttgt catttcggca cacgcaaagc aatcaaaaat
     2161 tatcgtcaat tcaaaaaata cgccatatcc tgatttttgc cttacttact ccgccatttt
     2221 ctattttgat ttaccatgcg attgattttt atcgtcaaat gccaaaatcg gaatgtcggc
     2281 ctgacggaac agcgcataaa cgggcttgag ccaataatct tccggaaggg aagccggttt
     2341 caatgcgtaa acgtgcacat tgggaaaatc cttcatcgtc aacgccgcac cgctgaaaaa
     2401 ggtataaatt tcatacctcg tatgcgggtt tttcttaatt tcgcgcaaaa tatagtcttc
     2461 gatgacatag ggcgaattta acgcggttac gccggaaagc ccgtaggtct ggcggggatg
     2521 cggcgcgaca tattgtatgt tgaaattttt tgccgccttt tccgaaattt ccttcatctc
     2581 tttgtcgggc gaacccaaaa gtatccgcac cgtgccgccc gtttcgtccc ccgccttcag
     2641 ttcggacgca tcgaacagcg gcaggtaagt catcttgcgg cggccgtcgt ccataatgtt
     2701 tttcaaaccc ttgaatatcg tgtaatgctc gtcggaagca ttgcgggttt tggcgatgct
     2761 ccaatccccg accatcattc gggcaaaatt ccgcttaatc gccccgttta cataaaactc
     2821 atcgcccaaa tagctgctct cccgtatcag gttgtttgtg ccgtcgtcaa aggtcttgat
     2881 ttccgcatcc gggtaagtac tcaaaaaggc ggcaatactg actttttcca aactcgccaa
     2941 ataaatccgc ctgaccttcg gcagcagcat cgacttcacc ttcagctccg ccatcgtcgg
     3001 aatgaaatta aacgatttgt tcaaaccgta gggcaggtag aaaaaatacg cccgctccgc
     3061 cttatccttt atctgattga aataataatc gtatttttca ttcctgtttt cagacatcag
     3121 caccacataa aaccgctccc ccggatgctg cgccataatc ctttctgcca ccttcatctg
     3181 caatatggta tagcagaaaa tcagattgac gggtttcccc tcttcattga agagtttgtc
     3241 cttcagcagg gaaaccgcgt tcctttcccc ctgatttacc cggtcaaacg tataaaatat
     3301 cccgaagcaa aaaacaatca ggcacaacac ggtcaaacag acttttttca accccatatc
     3361 cctaaaaact ccattccgac aaattgaaca tacgccccga gtgtcccggt gcggaaaatg
     3421 cggaatttta gcagatattc cgacagttgc cgaatattaa aatcaatttt accgaaaatg
     3481 tgccgccgca catcaggcac aacgccgccg acgccgccgc caaacgcggg aaagccattt
     3541 ctcccttcct ttcccgccgc cgcgttcaaa cacgccaaga tcgaacaaag cgcgaacaaa
     3601 gccgccaaaa tatgccataa tagccgcgca cattccccgc gcaatgtgtt tcaaaagaaa
     3661 aaaccaacct acaagagaat aatcctatga ctcaaaaatc caccattgtt tatacccata
     3721 ccgacgaagc ccccgcgctg gcgacccaat cgctgctgcc gattgtgcag gcgtttgccc
     3781 gccacgccga tattgatgtc aaaactgccg acatttccct gtccggccgc attttggcgg
     3841 cattccccga atatctgacc gaagcgcagc gcgtacccga cgcgcttgcc gaattgggcg
     3901 aactggtgaa acaacccggt gcaaacgtaa tcaaactgcc gaacatcagc gcatccgtac
     3961 ctcaactgac cgccgcgatt aaagaattgc agtctaaagg ctttgccgtt cccgactatc
     4021 ctgccgaccc tcaaaccgat gaagaaaaag ccgtacgcga acgctacgac cgcatcaaag
     4081 gcagcgcggt aaaccctgtc ctgcgcgaag gcaactccga ccgccgcgcg cctaaagcgg
     4141 tgaaaaactt tgccaaaaaa aatccgcaca gcatgggcgc gtgggcgaaa gactccaaaa
     4201 cccacgttgc caccatgcaa agcggcgact ttttccataa cgaacaatcc gttaccgtac
     4261 ccgatgcgac ttccgtatcc atcgtgttca ccgacaaaca aggcaataaa aaagagctgc
     4321 gcgagcctgt tgccctgaaa gccggcgaaa tcattgacgc ggccgtaatg agcaaaaaag
     4381 ccctgctcgc cttccttgcc gaacaagtga aagacgcaaa agctaaaggc gtattgttct
     4441 cgctgcatat gaaagccacg atgatgaaag tgtccgaccc gattatcttc ggacacgccg
     4501 tcaaagtgtt cttcgcgcct gtatttgaaa aattcggcgg caaactggct gccgcaggcg
     4561 tcaacgtcaa caacggcttc ggcaacctga ttgccaatct ggacaagctg gatgcggaca
     4621 cccgcgccgc cgtcgaagct gaaatcgccg ccgtttacgc cgccaaccct gatttggcga
     4681 tggtcgattc cgacaaaggc atcaccaacc tgcacgttcc cagcgatgtc atcgttgatg
     4741 cttctatgcc tgcgatgatc cgcaattccg gccgtatgtg ggacaaaaac ggcaaagcgc
     4801 aagacaccaa agccgtgatt cccgacagca gctatgccgg tgtttaccaa gcaaccatcg
     4861 atttctgccg cgaacacggc gcattcgatc cgacaacaat gggtactgtg cccaacgtcg
     4921 gactgatggc gcaagcggcg gaagaatacg gctcacacaa caaaaccttt gaaatcgaag
     4981 ccgacggtca ggttcaagtg attgatgcgg cgggcaatgt cctgatgcag cacgacgttg
     5041 aggcaggcgg catctggcgc atgtgccaaa ccaaagacgc tcccgtcaaa gactgggtac
     5101 aactcgccgt caaccgcgcc cgcctgagca acacgcccgc cgtgttctgg ctcgacgaaa
     5161 accgtccgca cgacaagagt ctgctcgcca aagtcaaagc ctaccttgcc gaattggata
     5221 ccaacggcct cgacatccgc gtcctcgcgc ccgaagaagc cgctaaattc agcttgggcc
     5281 gtctgaaaaa cggcgaagac accatttccg taaccggcaa cgtcctgcgc gattatctga
     5341 ccgacttgtt cccgattttg gaactgggta ccagcgcgaa aatgctgtcc atcgttccat
     5401 tgatgaacgg cggcggtatg tttgaaaccg gcgcgggcgg ttctgcaccg aaacacgtcc
     5461 aacagttcct cgaagaaaac cacctgcgtt gggactcatt gggcgaattc ctcgcgcttg
     5521 ccgtatcgtt tgaacatctg gcgcaaaaaa ccggcaacgc caaagcccaa gtcctcgccg
     5581 acactttgga tgccgccacc gaaaaactgc tgttgaacga caaatcgccc aaacgcaaag
     5641 cgggcgaact cgacaaccgc ggcagccatt tctacctcac cctctactgg gcgcaagaat
     5701 tggcggcgca agacaaagat gccgaactga aagccgcatt tgctccattg gcagccgctt
     5761 tgactgccga cgaagccaaa atcgttgaag aactctctgc cgtacaaggc aaagcggtcg
     5821 acatcggcgg ctactacgca gccaatcctg aaaaagccgc acaagcgatg cgtccgagcg
     5881 caacctttaa tcaagtacta aacgccttat aagcacaaag gcaaaaatgc cgtctgaaca
     5941 ttcgtcgttc agacggcatt agttatccct atctgcctga ttatgattaa acgccgcacc
     6001 cattggatat atagtggatt aacaaaaacc agtacggcgt tgccccgcct tagctcaaag
     6061 agaacgattc tctaaggtgc tgaagcacca agcgaaccgg ttccgtacta tttgtactgt
     6121 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tataaatcat ataatcttta
     6181 gttttttacc ctacggaaag acaataatat gaaaactgtt ccaaccatcc tgaccggcat
     6241 ccttttggca accgcccttc ccgcatccgc acacggtatg cataagagca aacccctggc
     6301 tatggacgag ctgccgccga tttgccaaca atatttcaaa cgcaccgaaa cctgttacaa
     6361 caaagccgga aacaaagcag atttcgcacg caacaacacc aaattcctat tccaagccct
     6421 acctgccgcc gatttgggac agcgtaagca aatgtgccaa atcgctatgg attcgtttgc
     6481 agaaaaaacc cgcaatctga attgcgaata aagccgcatc agcaatagca atgccgtctg
     6541 aaacaaaggt tcagacggca ttctcacatc aaaacgtacc ggctgataaa acccgccttc
     6601 cccgcatccc tgacccgaac aggattaaac ggagtaacgg tttcggccat tatggcaagt
     6661 ccccatccca aaaatgacca tataaattaa taccataaaa cattgataaa aagcgtctgc
     6721 cggttgcggt ttgttgcagg gcgttacgga aaatcatcgt aaaccgatga tttttatata
     6781 gtaattgaca caacctgaac aaaaaagaac cgccctgaat cagggcggtt ttgctttgcg
     6841 gcggaaacgg caggattcga ctaaatttta ttttattgat ttaaatacat ttatttctta
     6901 taaaaattta atttaccata aaaacagcca tatacaaaaa tcttggagta actactgcat
     6961 tacactatta gaaggatgca ggg
//
LOCUS       DAWTCI010000096         6803 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWTCI010000096 DAWTCI010000000
VERSION     DAWTCI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.096"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            89..493
                     /locus_tag="WM481_001920"
     CDS             89..493
                     /locus_tag="WM481_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HFA9081171.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWHG
                     HIAEITDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DATFPAPIWANPVRKHGMTLPQNWTEQLKKLL"
     gene            533..1714
                     /locus_tag="WM481_001921"
     CDS             533..1714
                     /locus_tag="WM481_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFA9081172.1"
                     /translation="MTEPTSRRRFLKTCTAAGAGLLQACGTSATSVPPLPSSHSVVKA
                     RTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRFQ
                     RFAGTDAQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGTL
                     FFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGTTQNRLTVDV
                     PYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNTL
                     YLSGILGKQRAIVFGDFRMEKIRDLYDSSYDFSAVAKHISRTAKIPVLTGFPFGHIAD
                     KITFPLGAHTRIRMNGNGGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADIS
                     E"
     gene            complement(1816..4071)
                     /locus_tag="WM481_001922"
     CDS             complement(1816..4071)
                     /locus_tag="WM481_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFA9081173.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     AYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNARFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAPDLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            4444..5622
                     /gene="nirK"
                     /locus_tag="WM481_001923"
     CDS             4444..5622
                     /gene="nirK"
                     /locus_tag="WM481_001923"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HFA9081174.1"
                     /translation="MKRQALAAMIASLFALAACGGEQAAQAPAETPAASAEAASSAAQ
                     ATAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGSGAASAPAASAPAASAPAASASEKSVY
                     "
     gene            5800..6555
                     /locus_tag="WM481_001924"
     CDS             5800..6555
                     /locus_tag="WM481_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFA9081175.1"
                     /translation="MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNISWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKDRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGAS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCASR"
BASE COUNT         1701 a         1923 c         1709 g         1470 t
ORIGIN      
        1 tggtacaatt aacggcaatg cggacagaca aattaaacta tcagaaattg ccctaccgtt
       61 ttttaaacac tttcaggaat aaggaaaaat gaccgcccaa ccctgcccca tctgcacggc
      121 acaagatgaa gatattttgc tgcaaacccc caagctgcgc gtcatcgccg tccataacga
      181 cagcggttcg ccggcattct gccgcgtcat ttggcacgga cacattgccg aaattaccga
      241 cctttcggca gcggaacgcg gcgaattgat ggaaatggtg tacaaagtcg aagccgctat
      301 gcgccaagtg ttccggccgg caaaaatcaa cctcgccagc ttgggcaatg tcgtgccgca
      361 cctgcattgg catattatcg cccgctttga aaacgatgcg actttccctg cgccgatttg
      421 ggcaaacccc gtccgaaaac acggtatgac cctgccgcaa aattggacgg aacagcttaa
      481 aaagctgctt taaacccgcc gatgccgtct gaaaccgtat gaaagggaaa ttatgaccga
      541 accgacctcc cgccgccgtt ttctgaaaac ctgcaccgcc gccggcgcgg ggctgcttca
      601 ggcttgcggc acatccgcca catccgttcc gccccttccc tcttcccatt ccgttgtgaa
      661 agcccgaacc gtgcctctcc aaacgccacg ccgtcaaagt tcggacggca accttctgcg
      721 cgttgtcgct tcgtcaggat ttgccgaaga cactaaccgc gtcaacacag ccttaacccg
      781 cctttacaat gccggtttta ccgtaaccaa ccaacaggcg ggcagccgcc gtttccaacg
      841 gtttgccggc acggacgcgc aacgcgcggc agatttccaa gaggtcgcct ccggccgcgt
      901 cgccacgcct aaagtgctga tgggcttgcg cggcggttac ggtgcggcgc ggattctgcc
      961 gcatatcgat tttgcctcgc tcggcgcaag gatgcgcgaa cacggcacgc tgtttttcgg
     1021 attcagcgac gtatgcgccg tccaactggc attgttggca aaaggcaata tgatgagttt
     1081 tgccggcccg atggcttata gcgattttgg caaacctgcc cccggtgcgt ttacgatgga
     1141 tgcctttatc aaggggacaa cccaaaaccg cctgaccgtt gatgttcctt atatccaacg
     1201 cgccgatgtc gaaaccgaag gcacgttgtg gggcggcaac ttaagcgtcc tcgcctcgct
     1261 cgccggtacg ccttatatgc ccgacatcga cggcggcatt ttgttcctcg aagatgtcgg
     1321 cgaacagccc taccgcatcg aacgtatgct caatacgctg tatctttcgg gtattttggg
     1381 caaacagcgc gccatcgtgt tcggcgattt ccgtatggaa aaaattcgag atctctatga
     1441 ttcgtcttat gatttttccg ccgttgccaa gcatatttcg cgcacggcga aaatccccgt
     1501 gctgacgggc ttcccgttcg gacacattgc cgacaaaatc actttccctc tgggcgcgca
     1561 cacccgaatc cgtatgaacg gaaacggcgg ctattcggtc gcgtttgaag gctaccccac
     1621 actcgatgcg tccgccctga ctttggatac cctgctccca ccgccggatt tgcctatctt
     1681 ccccgaaagc ggtgttgccg atatttcgga ataaacccgc aaacggacaa atgccgtctg
     1741 aagccttcag acggcatttt cctgtaaaaa aaccgcacct gatggcaggt gcggtttcca
     1801 ttttcaggct aaaacttatt tgtcgcggcc gaatacgatt ttagtggctt ggatggcaac
     1861 gcagattgca ccgccgataa agattaagtc gggggcagtg cgtacccagc gcagggtgtc
     1921 gaggatttcc atttgcagga attcttcgct gcgggcatac cacaaaccgt gcgtgataga
     1981 ggcgtatgcc tgaatcacgc cgacaggcag caggctgatg gcaatcatac cgaccaagcc
     2041 gccgttgagc agccagaagc cccaagtcat cagtttgtcg tcaaaccgcg cgttcggttt
     2101 caagtagcgc gcaaccaaca atacgaagcc caatgccaag aaaccgtaca caccgaacaa
     2161 ggcggcgtgc gcgtgaacgg cggaagtgtt caaaccttgg atatagaaca gggaaatcgg
     2221 cggattgatc agaaagccga atacgccggc accgatcata ttccaaaaag cgactgccac
     2281 gaagcacatc agcggccaac gcaggcgttt cgcccagtcg gacaggtgtt ggtaagacca
     2341 gtgctcgtat gcctcgcggc ccagcaacac cagcggcacg acttccaaag cggagaagca
     2401 ggcgccgatt gccatagagg cggaggtaga gccggagaag tacaggtggt gcagcgtgcc
     2461 cggaacgccg cccaacataa agatggcggc agcggccaaa gtggaggcag tggcggtact
     2521 gcggcggaca aagcccatat tgtagaagac aaaggcaaag gcggcagtgg caaatacttc
     2581 aaagaagcct tccacccaca ggtggaccac ccaccaacgc cagtattcca ttacggcaat
     2641 cggggatttt tcgccataga acaggcccgg tgcgtagaac acgcccacgc cgaccataga
     2701 agcgacaaag attgccagca ggtttttgtc cacgcctttt tctttgaagg cggaaaccgt
     2761 gcagcgcaac atcaggaaca gccacaacag cagtccgacc atcaaaagga gttgccagaa
     2821 acgtcccaaa tcgaggtatt cgtaaccttg gtgtccgaac cagaagttaa attcgggggg
     2881 aaggatgtgc gtcaacgcga agaagttgcc cgcgtaagaa ccgccgacca cgatgaagag
     2941 ggcgatatag aggaagttca cgcctgcacg ttggaacttg ggatctttgc cgccgttgac
     3001 aatcggcgcg aggaacaaac ctgccgtcaa aaagccggtt gcaatccaga agatggcgga
     3061 ttggatgtgc caagtacggg tcagggcgta ggggaaccag tcggacattt caaagcccaa
     3121 cgcctcgtca atgccgtaga aaccctgacc ttcgacggtg tagtgcgcgg tcaggccgcc
     3181 cagcaatact tgtaccacaa acagggcgac cgtcaggaag acgtatttgc ccaatgcttt
     3241 ttgcgaaggg gtcagttgga ttttggaaat cgggtcttca gacggcactt ccacttcctc
     3301 gtgtttggtc aggaaggaat aaccccacat caacaaaccg atgcccatca agagcagtac
     3361 gacactggta aacgaccaca tgtagttttc agtagtcggt acattgttga tcaaaggctc
     3421 gtgcggccag ttgttggtgt aggtaaagac ctcgccggga cggttggtcg aagcagacca
     3481 agaagtccag aagaagaagt cgaacagttt ttcacgcgct tcttggcttg gcaatgtgtt
     3541 gtttttcatt gcaaagtgtt cgcgggtggt ttgcaacttg ggatcatcgc cgtaaacgcc
     3601 gtgatagtaa ggcaggatgc tttcgatggc tttcacgcgc gtatcgctga tgacgacgct
     3661 gccgtcttct ttaatacggc tttgattgcg gtattcatcg gccaagcggg ttttcagaac
     3721 ggcttgttct tcaggggaaa cttcatcgaa ttttttgccg taagcctgtt gcgcggtcaa
     3781 atccaaccag gcggacaact cacgatgcag ccagtcggcc gtccagtccg gagcctgata
     3841 tgcgccgtgt cccagaatcg aaccgacttc catgccgccg gtagtctgcc acgcagactg
     3901 acctgccaaa atatcgtctt tcgtcatcag cactttgccg gatgcggaaa cgacctgttc
     3961 ggggtaaggc ggggctttct tataaacctc gctgcccata tagccaagaa tggtaaagca
     4021 taccgccaga acggcaaaca gcaagtacca cagcttcttg tactgtccca ttttgagagc
     4081 tccttttaat aaatcagttg tttaaaattc acaaaatatg aatgttaaag attgtagcat
     4141 ggtttaccgc gcaaataaac atttgttcaa acaaactcac atatagaaca aatacatata
     4201 tgataataac tatcattatt ctttactcgg aaattgccct gcctttgcct gctctgccgg
     4261 agcccctagc aaatcagcct attcattgta atttttagta gttataaagt attagaagcg
     4321 tcattttaag ttcatatttt atgaattatt tgacttaaat taaaatgccc gcaatgggac
     4381 aaccgcataa tcacaccaag ttcttaacta atccccctac ttttcttaca aaaggaaaac
     4441 attatgaaac gccaagcatt agccgcaatg attgcttcct tattcgcatt ggccgcctgc
     4501 ggtggcgaac aagctgctca agcccctgcc gaaacccctg ccgcttccgc agaagccgca
     4561 agttccgccg cacaagctac cgccgaaacg cctgcaggcg aactgcccgt catcgatgcg
     4621 gtgaccaccc acgctcccga agtacctccc gcaatcgacc gcgactatcc tgccaaagta
     4681 cgcgtaaaaa tggaaaccgt cgaaaaaacc atgaaaatgg acgacggggt ggaataccgc
     4741 tactggacat ttgacggcga cgttccgggc cgtatgatcc gcgtacgcga aggcgatacg
     4801 gttgaagtcg aattctccaa caatccttct tctaccgttc cgcacaacgt cgacttccac
     4861 gccgcaaccg gtcagggcgg cggtgcagcc gcgaccttta ccgccccggg ccgcacttcc
     4921 acattcagct tcaaagccct gcaaccgggc ctgtacatct accactgcgc cgtcgcgccg
     4981 gtcggtatgc acatcgccaa cggtatgtac ggtctgattt tggtcgagcc taaagaaggc
     5041 ctgccgaaag tggataaaga gttttacatc gtccaaggcg acttctacac caaaggcaaa
     5101 aaaggcgcgc aaggcctgca accgttcgat atggacaaag ccgttgccga acagcctgaa
     5161 tacgtcgtat tcaacggcca cgtaggcgct atcgccggcg acaacgccct gaaagcgaaa
     5221 gcaggcgaaa ccgtgcgtat gtacgtcggt aacggcggcc cgaacttggt gtcttccttc
     5281 cacgtcatcg gcgaaatctt cgacaaagtt tatgttgaag gcggcaaact gattaacgaa
     5341 aacgtacaaa gcaccatcgt gcctgccggc ggttctgcca tcgtcgaatt caaagtcgac
     5401 atcccgggca gctacacttt ggtcgaccac tccatcttcc gcgcattcaa caaaggcgcg
     5461 ttggggcaat tgaaagtaga gggtgcggaa aaccctgaaa tcatgactca aaaattgagt
     5521 gataccgctt acgccggcag cggcgcggct tctgcccctg ctgcttccgc accggctgct
     5581 tctgccccgg cagcctctgc atccgaaaaa agcgtttatt aaatcggata cccgtcatta
     5641 gcgggacaaa ccactgccgc cgtacttcat tacgcacggc agtggttttt taacaaccaa
     5701 tctttccttt cggaagattg atttttcaga cggcattcgc ggctttttgt ttaaaatgcc
     5761 gtctgaaaaa tcaatgctaa ccgcctgtca ggaggcttta tgaagtacgt ccggttattt
     5821 ttcctcggca cggcactcgc cggcactcaa gcggcggctg ccgaaatggt tcaaatcgaa
     5881 ggcggcagct accgcccgct ttatctgaaa aaagataccg gcctgattaa agtcaaaccg
     5941 ttcaaactgg ataaatatcc cgttaccaat gccgagtttg ccgaatttgt caacagccac
     6001 ccccaatggc aaaaaggcag gatcggttcc aaacaggcag aacccgctta cctgaagcat
     6061 tggatgaaaa acggcagccg cagctatgcg ccgaaggcgg gcgaattgaa acagccggtt
     6121 accaatattt cctggtttgc cgccaacgcc tattgcgccg cacaaggcaa acgcctgccg
     6181 accatcgacg aatgggaatt tgccggactt gcttccgcca cgcagaaaaa cggctcaaac
     6241 gaacccggct acaaccgcac tattctcgat tggtatgccg acggcggacg gaaaggcctg
     6301 cacgatgtcg gcaaagaccg cccgaactac tggggtgttt atgatatgca cgggctgatt
     6361 tgggaatgga cggaagattt caacagcagc ctgctttctt ccggcaatgc caacgcgcaa
     6421 atgttttgca gcggcgcatc tgtcggggcg agcgactcgt ccaactatgc cgccttcctc
     6481 cgctacggca tccgcaccag cctgcaatcc aaatatgtcc tgcacaactt gggcttccgc
     6541 tgcgcaagcc gataacccct tcaattatag tggattaaca aaaaccagta cggcgttgcc
     6601 tcgccttgcc gtactggttt ttgttaatcc gctatattcc gccatctcta agatttacag
     6661 cgatacacgg gtaatttaag gaatgcccga accgtcattc ccgccacttt tcgtcattcc
     6721 cgcgaaagcg ggaatctgga atctcggact ttcagataat ctttgaatat tgccgttgtt
     6781 ccaaggtccg gattcccgcc cgc
//
LOCUS       DAWTCI010000097         6582 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWTCI010000097 DAWTCI010000000
VERSION     DAWTCI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.097"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            172..686
                     /locus_tag="WM481_001925"
                     /pseudo
     CDS             172..686
                     /locus_tag="WM481_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
     gene            683..1009
                     /locus_tag="WM481_001926"
     CDS             683..1009
                     /locus_tag="WM481_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFA9081176.1"
                     /translation="MTDAENGRGQDTLAHIAQSIRNILFTRIGTRLMREEYGSFIPDL
                     IDMPAGHAAIALIHQAAVTALARWKPRITVRRIQADTADLAAGKIKLTLDVTLADGGE
                     RTYRIK"
     gene            1030..2085
                     /locus_tag="WM481_001927"
     CDS             1030..2085
                     /locus_tag="WM481_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFA9081177.1"
                     /translation="MGNSRLSQLPAPAAIEETDFEGIFARKKAALTALCPESIRETVA
                     QTLELESEPLTIDLQQQAYQELLVRNRINEAVKANLLAYAQGSDLDHIAAQYGLSRKT
                     IRAADPDANPPVAAEYETDDAFRARVQAHPEKYAAGPRTAYEAHAIDAHPQITHARAV
                     RRAAGTVEVYIKTQSGTPDETILTAAREYLSAETRRPLCDNVQVTAAQPKDAAVEYSA
                     EYHPAANIQAERQTACEALDNLWRQNAHIGASVALSKIIGALDTPGVKKNHTAQPRRR
                     HRMRQRRIHPNHVHARARINEQHRPREQQPPATRTGKADRTRNCRRLPPTRPRIFTLS
                     RLRKKHRHGRGLGLCRN"
     gene            2504..3009
                     /locus_tag="WM481_001928"
                     /pseudo
     CDS             2504..3009
                     /locus_tag="WM481_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3441..4691
                     /locus_tag="WM481_001929"
     CDS             3441..4691
                     /locus_tag="WM481_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFA9081178.1"
                     /translation="MEKTQFGRHPRRRDRVLSQSRPKPRRLSQSRRHDLCTKHLLDLY
                     RALGNTNKLPDLTRTDIGITAWFPSDQIQTGWLAFDDIRERVTESAYPELYRLLTEKY
                     GRIQNVPQAEDRFILNAGNGLAVGTKQEDEIKRHVHKVFSHWANHPDAAAVGYEDRNE
                     RQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKAADTLGEAVF
                     RIKSHGETANAGALDASRLAQGLQEKADRDHTHTTAQIQGLDEKISAAVAAQFTRQTI
                     GGVDIVRFPDGTMIQTGSYRFARGGSPIGNEVVFPIAFADGNVKCFVSERHSGRVNGE
                     RQHNWLFIRAKNHAAAIITNWYESSCDWMAIGKSASGNAASPTPIVPEIPEIDEEPQR
                     ESGRSSTGLRNPRRHRGLDFPVGS"
     gene            4803..5132
                     /locus_tag="WM481_001930"
     CDS             4803..5132
                     /locus_tag="WM481_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081179.1"
                     /translation="MPRQSCHSSPFPRKRESGTQTRQESIGKNNPTAVIPAQAGIQTH
                     NVKAVYQKKPKPNALDFRLRGNDEELGSDERREQPRKKPPTPSDGIGNKNRTAETARE
                     RIAGRAR"
     gene            complement(5148..5459)
                     /locus_tag="WM481_001931"
     CDS             complement(5148..5459)
                     /locus_tag="WM481_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HFA9081180.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMKTGAR
                     T"
     gene            complement(5467..5749)
                     /locus_tag="WM481_001932"
                     /pseudo
     CDS             complement(5467..5749)
                     /locus_tag="WM481_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5831..6444
                     /locus_tag="WM481_001933"
                     /pseudo
     CDS             5831..6444
                     /locus_tag="WM481_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1771 a         2262 c         1572 g          977 t
ORIGIN      
        1 cattttttac ccacccccaa acctaaaaac cacaaccaag ccctacaccg cttgccccca
       61 cctcgccgcc gccccaaaat aacctcactc tgttggaagc ctgccggaaa cggcaaaagc
      121 cgccccgttc cgccattacg gggcggtttc ccacatcaag acgagacaga aatgaccgac
      181 cgccaaatag acaacctgat caaacccgcc accatcgccg acaccgaccc cgcgtccgcg
      241 cacaacacgg cggcattacc accgactggc tgccctatat cgtccccttt gcaggcggcg
      301 tgtcagtatg gcgcatccca agtgtcggcg aagcctgcac catcttatcc cctgccggcg
      361 aacccgagaa cggcgtagtt ttatgctgcc aagcctccga ccgatacccc gccccgtccg
      421 ccgaccccgc cgaaaccgtc gtccgatttc ccgacggcac gcacatccgc tacaaccaca
      481 acagcggcgc gatggaatta aaagccgtta caagcctgac catcgacacc ccccaaacca
      541 ccataaccgg acacctgacc gtcaaccaaa ccaccaccgc ccaagggctg ctgacctacc
      601 aaaatggcat gaacggccaa ggcggcagcc tgtccgagca cacccacccc gacgactccg
      661 gcggtacaac ggaaaaaccc caatgaccga cgcagaaaac ggacgcgggc aagacaccct
      721 cgcccacatc gcccaatcca tccgcaacat cctgttcaca agaataggaa cgcggctgat
      781 gcgcgaagaa tacggcagtt ttattcccga cttgatcgat atgcccgccg gtcacgccgc
      841 catcgccctc atccaccaag ccgccgttac cgccctcgca agatggaaac cccgcataac
      901 cgtccgccgc atacaggcag acaccgccga cctcgccgcc ggcaaaatca aattaaccct
      961 agacgtaacc cttgccgacg gcggcgaacg aacctaccgc atcaaataat aaaaacaaca
     1021 ggataaacaa tgggaaacag ccgattaagc caactccccg cgcccgccgc catcgaagaa
     1081 accgactttg agggcatctt cgcgcgcaaa aaagccgccc tcaccgccct atgtcccgaa
     1141 agcatccgcg aaaccgtcgc ccaaaccctc gaattagaat cagaacccct aaccatcgac
     1201 ctgcaacagc aagcctatca agagctgctc gtccgcaacc gcatcaacga agccgtcaaa
     1261 gccaacctct tggcatacgc acaaggcagc gacctcgacc acatcgccgc ccaatacgga
     1321 ctttcacgca aaaccatccg cgccgccgac cccgacgcga acccgccggt tgccgccgaa
     1381 tacgaaaccg acgacgcatt ccgcgcccgc gtccaagccc atcccgaaaa atacgccgcc
     1441 gggccgcgca ccgcatacga agcccacgcc atcgacgcac acccccaaat cacacacgcc
     1501 cgcgccgtgc gccgcgccgc cggcacggtg gaggtttaca tcaaaaccca aagcggcacg
     1561 cccgacgaaa ccattttgac cgccgcgcgc gaatacctgt ccgccgaaac acgccgcccc
     1621 ctttgcgaca acgtgcaagt aaccgccgcc caacccaaag acgcggcagt agagtattcc
     1681 gccgaatacc accccgccgc caatattcaa gccgaacggc agaccgcctg cgaagccttg
     1741 gataacctat ggcggcaaaa cgcccacatc ggcgcatccg tcgccctgtc taaaatcatc
     1801 ggcgcgttag acacccccgg cgtgaaaaaa aatcacactg cacagccccg ccgccgacat
     1861 cgaatgcggc aacggcgaat acatccgaat cacgtccacg ctcgagcgcg aataaatgaa
     1921 cagcaccgtc cccgcgaaca acagccccct gcaacacgca ctggcaaagc tgacagaacg
     1981 cgaaactgcc gccgtctccc gccaactcga ccccggattt ttaccctttc acgccttcgc
     2041 aagaagcatc ggcacggaag agggctggga ctttgccgaa actgacgaag cccgccgcaa
     2101 cctcatcgca ggctttgccg aaatccacgc ccgaaaaggc acgccgtacg ccatccgcgc
     2161 cctcttcccc atcttgcggc tgggcgaaat ccaaattatc gaacgcgacg gcgagttcaa
     2221 gtgggacggc tcggtcttgt tcgacggcag ccgcacattc ggcaggcgca agggtgactg
     2281 ggcggaatac cgcattgtct taacgcgccc cgtcagcatc cgccaaaccg cccgcatccg
     2341 cgccatgttg gcggaaatcg cccccttgcg gtgcgaactt accgcgctcg actaccgcaa
     2401 ccatccccac cgctggaacg gcaaaatccg ctttaacggc gaatacggtt tcggcacgac
     2461 ataacgcgcc cccgaaaatc aacaaacaaa aggaagcccc aaaatggcaa acgcaaccga
     2521 acaaaaccaa ttcgaccaag ccgtccgcct catcgaaccc ggcgacagcg tcgtcgtcgg
     2581 cccaggcgcg cccgtcaacc aaccactgca agccctcgcc aaccgcaccc ttttgctcaa
     2641 aaaccaaacc gaagcccttc agacggcatc cgacaccaaa gccgcaccat cgcactgggc
     2701 gcgcccgggc aaatcaccgc aacaagccaa aacaccgtcc caaaaaacgg ccacacccac
     2761 gccatcgaca ccgcacgcac cgaccgcgcc ggcatcgtcc gattagacaa tgccattagc
     2821 gaagccgaag acaccgccgc cacccccaaa gccgtcaaaa ccgcgatcga ccaagcccgc
     2881 gccgccgccg cgaccgccga cctcaaagtc tccctctccg acaaccaaac cgttaccggc
     2941 caaaaaacct ttaccgccga aacacaattc caaagcggca tccggctgtc cgccaacccg
     3001 acgcactgaa acggcggcta caaagcctat atcggcgcgg ataacgaaaa cgcccacatc
     3061 gtcttcggcg acgacaccct gcgcctgcac ggcgcaaata accgcatttc ctacaacaac
     3121 cacgacatct tccacaaagc caacaaaccg cgtttcaacg aagacatcga aggcaaaccg
     3181 aacacgctgt ccggctacgg catcggcaac ttcaaagtcg aaacattccg gggcgatttg
     3241 aacagcctca aaaccgacgg cgtctattcc ctgccgacgg cggtcggcag ctcgaacctg
     3301 cccgttgaaa acaccgcctg ccatatccaa gtcatcgccg gcacgcaacc cggctggtgc
     3361 aggcagttgg gctatcccgc ctacacgtcc gacgtgtacg aacgctacca gaccagcagc
     3421 gcaaacgacg actggagcgc gtggaaaaaa ctcaattcgg aaggcatccc cgccggcgcg
     3481 atcgtgtcct ttcccaaagc cgtccgaaac cccgcaggct atctcaaagc cgacggcacg
     3541 atctttgcac aaaacacctt ctcgaccttt accgcgccct gggcaacaca aacaaactgc
     3601 ccgacctgac ccgtaccgac atcggcatca ccgcgtggtt tccgtccgac caaatccaga
     3661 cgggctggct ggcgtttgac gacatccgag agcgcgtaac cgaatcggct tatcccgaac
     3721 tttaccgcct gctgaccgaa aaatacggac gcatccaaaa cgtcccgcag gcggaagacc
     3781 gctttatcct caacgcgggc aacggcttgg cggtcggaac gaagcaggaa gacgaaatca
     3841 aacggcacgt ccacaaagta ttttcacact gggcaaacca cccagacgcc gccgccgtcg
     3901 gttacgaaga ccgcaatgaa aggcaaagaa gcgcgctcgt atcgacttgg acggacggcg
     3961 acttaaacga caacggcttt ttaaccccgc gattggacag caaaatggca acgggcggcg
     4021 cagaaaaccg ccccaaagcc ctggttttaa aactgtgcat caaagccgcc gacaccttgg
     4081 gcgaagccgt gttccggata aagtcccacg gcgaaaccgc caacgccggc gcactggacg
     4141 cgtcaaggct ggcgcaaggt ttgcaggaaa aagccgaccg cgaccacacc cacaccaccg
     4201 cccaaatcca agggctggac gaaaaaatca gcgccgccgt tgccgcgcaa ttcacacgcc
     4261 aaaccatcgg cggcgtggat attgtcagat tccccgacgg cacaatgata cagaccggca
     4321 gttacaggtt tgcaagaggc ggcagcccca taggaaacga agttgtcttc cccatcgcct
     4381 ttgccgacgg caacgtcaaa tgcttcgtat ccgaacgcca ttcgggacgc gtcaacggcg
     4441 aaaggcaaca caactggctg tttatccgcg caaaaaacca cgccgccgcc attattacca
     4501 actggtacga aagcagttgc gactggatgg ccatcggcaa atccgcctcg ggaaacgccg
     4561 ccagccccac cccgatagtc cccgaaatac ctgaaatcga tgaagaaccg caaagagaga
     4621 gtggaagatc atcaaccgga ctccgaaacc cccgccgcca ccgaggcctc gacttccccg
     4681 tggggtcgta agactagcgg gcggcagggc aggctgtaga gacgggctgt agagacgggc
     4741 ttcagcccac cgttccaagc aatccgaccg aaaccggccg cgccgccaat cctgcgaaac
     4801 ctatgccccg ccaatcctgc cactcttcgc cattcccgcg aaagcgggaa tccggaaccc
     4861 aaacgcggca ggaatctatc ggaaagaata acccgaccgc cgtcattccc gcgcaggctg
     4921 gaatccagac ccacaacgtt aaggcggttt atcagaaaaa accgaaaccg aacgccctag
     4981 atttccgcct gcgcgggaat gacgaagagt tggggagtga cgaaaggcgg gaacaaccgc
     5041 gcaaaaagcc gccgactcct tcagacggca tcggcaataa aaaccgcacg gccgaaaccg
     5101 cgcgggaaag gatagccggg cgcgcccgat aagcagcggc cgcagggtta agtccttgca
     5161 cctgttttca tcgaagacaa aggcggttcg ggaaataagg gcgcaatatc aaaagttttg
     5221 cgttgcctga tttgccacaa atcataaccg gcctgcatat tcagccaaaa atcaggcgaa
     5281 gtgttgagca gttggcttaa acgaaccgcc atctcgggcg taatcgaaga ttgggcgttg
     5341 acaatgcgcg ataaggcaac ccggctgaca cccaggcgtt tcgctatatc ggtaatgctt
     5401 gcgccgttaa tatactcttt taaaacaagg cccgggtggg caggattgtg cattttcata
     5461 ttattcctag tgataatcct gataatcgac aacttcgaca ttgccgtcgt tgaaacgaaa
     5521 agtcaaacgc caatttccgt tgactttgaa ccgcccaatg tcccgacaaa ctgcccttga
     5581 ggggatgaag attccaactc ggcacagcca tatccgaagg cgtttgcgcg gcattcagcg
     5641 cggttagaag cagattcagt ttgacggaat gcccggcttg gatgcccgat agcgagcctg
     5701 ttttaaaaaa gcgttcaagc cctttgtgtt tgaaagaaac aatcatcatc ccaccccgtc
     5761 tgtatcgcca aaggatacaa aacttttatt tgattgcaaa gcgacgatat acaaaaaagg
     5821 aaaccccgaa atgaccatct atttcaaaaa cggcttttac gacgacacat tgggcagcgt
     5881 ccccgaaggc gcggttgccg tcggcggcga agagtacgcc gccctcttcg caggacaggc
     5941 gcagggcggg caaattgccg ccgattccga cggccgcccc gttttaaccc cgccgcgccc
     6001 gtccgagtac cacgaatggg acggcaaaaa atggaaaatc agcaaagccg ccgcccgttt
     6061 cgccaactcc cgtttcgcca aacaaaaaac cgccttggca ttcagcctcg cggaaaaggc
     6121 ggacgaactc aaaacagcct cttggcgggc tatccccaag tggaaatcga cagcttttac
     6181 aggcaggaaa aagaaaccct cgcgtggcag gcggacaacg acgccccgac cccgatgctg
     6241 gcgcaaatcg ccgccgcaag gggcatagaa ttggacgttt tgattggaaa ggttgtcgaa
     6301 aaacccgccc gcctggccgt tgccgtcgac gcgattatcg gaaagcggca gcaacttgaa
     6361 gatagattaa ataccatcga aaccgcgcca gcattagatg cgttagaaag ggaaatcaaa
     6421 gaatgggcgc taaacgtcgg ctgagagaat atatttatca tctactagtg gccatagacc
     6481 aactgttcaa cgctataacc ggcggcgcgg cagacgaaac cctctcaagt cgcacctatc
     6541 gcggcgcgcg gctcgcccaa caacccaaaa cccgctggaa ga
//
LOCUS       DAWTCI010000098         6573 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWTCI010000098 DAWTCI010000000
VERSION     DAWTCI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.098"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(61..258)
                     /locus_tag="WM481_001934"
     CDS             complement(61..258)
                     /locus_tag="WM481_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081181.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAV
                     NAGLEEIGNQCEFVVFGFLKD"
     gene            complement(263..553)
                     /locus_tag="WM481_001935"
     CDS             complement(263..553)
                     /locus_tag="WM481_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081182.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVTGFQFVKKG"
     gene            complement(598..1946)
                     /locus_tag="WM481_001936"
                     /pseudo
     CDS             complement(598..1946)
                     /locus_tag="WM481_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(2085..2507)
                     /locus_tag="WM481_001937"
     CDS             complement(2085..2507)
                     /locus_tag="WM481_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HFA9081183.1"
                     /translation="MTDIFTPSKRSFVMSKIHSKETKPEVLVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWHGHSCNKGHIPKSNMDFWLEKITKNRERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVEEIKDAV"
     gene            complement(2513..4156)
                     /locus_tag="WM481_001938"
                     /pseudo
     CDS             complement(2513..4156)
                     /locus_tag="WM481_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
     gene            complement(4170..4499)
                     /locus_tag="WM481_001939"
     CDS             complement(4170..4499)
                     /locus_tag="WM481_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9081184.1"
                     /translation="MAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPAPTITTKFFSI
                     SNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVPPMYAEKIGRA
                     IVDNIEC"
     gene            complement(4538..5146)
                     /locus_tag="WM481_001940"
     CDS             complement(4538..5146)
                     /locus_tag="WM481_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFA9081185.1"
                     /translation="MQVLAGIDYELSCKETYEANINAKFIQADIFELQPETLEKELGL
                     KKNDDDLILIGCSPCQYWSVIQTDKRKSEKSKSLLLEFQRFVEYFNPGYVVVENVPGI
                     LSRMKESGLDNFIKLLEEKGFTVHFGIHNTADYGIPQSRKRFTLIANRITKKSWNQSS
                     IRANGLRYAMFWEWKTAFPKLWQDIKTRRILCIVVRDYPISI"
     gene            5515..5661
                     /locus_tag="WM481_001941"
     CDS             5515..5661
                     /locus_tag="WM481_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081186.1"
                     /translation="MSRIYLPLLFPPHIVERGLLYFQQDKVRDVQKISLGRYRAKVFD
                     SENY"
     gene            complement(6038..6487)
                     /locus_tag="WM481_001942"
     CDS             complement(6038..6487)
                     /locus_tag="WM481_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFA9081187.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         1779 a         1504 c         1178 g         2112 t
ORIGIN      
        1 aaaaagtgtt tggcggtaat ggatggagag tttttcagac gaccgccgaa catccgaaaa
       61 tcagtctttc aaaaatccga atacgacaaa ttcgcattgg ttgccgattt cttccaaacc
      121 tgcgttaacc gcttcttcga agtcgtagaa ataatcggca ttggtaatta atttggtatg
      181 tccgatgtcg cccgtttcag gagagagata cagaaagtcc cctgttgata cggactggac
      241 aacatagact ttctgcattc aatcagcctt tcttaacgaa ttgaaaaccg gtgactttca
      301 gtttttgagt tttgcccgta gtgacgattt ccacgttcag gtttgcttcg atcggaaatt
      361 gggcgtttcg gaactgctcg aaattggcag agccgccgaa atcgtattca gtagtagagc
      421 tgcccaatgc gttgccttgg gagctgtcta agggtgtggc gacaatcagg cggcaatagt
      481 cgaagctctt gccttcgatt tgtccgttga attttttaac gccgacgatg tggccttgaa
      541 gttggatgtt cattttttgg tttccttgtg tgattaaacg tctttcgggc agacacttta
      601 agcccatgaa atcggtagtc ttgcgaattt gtcgtaaatg aagttgttat agctttcttc
      661 attgttgacg tgtttttgct gttcaagctg tttttcaaga ttctcgtaat attcgtacat
      721 ataataaggg tcattgtacg gtttgaatgc gggctgttca tgaatgactt gagatttcaa
      781 aaaggcgcag tcgtaggctt cgagagccaa agacttgggc agcttgtgat gactcggctc
      841 aatcagttca aacagtttgg ctttgtccaa ttcgggaaaa atgaatttca gaccgtttgc
      901 cgcacgtccg aactgttttt ttacccattc aaggcagcgg tcggctgaaa caaccttatc
      961 ttccttaacc gcgtgtatgc gcgttgcctt ttgggcgaat cgttcgcaaa tgggatatgc
     1021 gccgccgaaa tattcgcccg gattctgcaa aacttcgaaa gggataacga tgtcttttgc
     1081 tttgaattca atttcaaagc gcgtccatgt gcttgtttta tcgcccaact gcttgccttt
     1141 ttcatagacg cggacatatt tggacgattc acgggagccg ataccatagg tcttgccttt
     1201 ggtcattttg gcttcatcgt cttcttccca atccgacccc aaacattcgc ccttcggttt
     1261 gacgtgatgg cacgtaaaca aacctttatt gcggtcttct cttgcctgat tcgggctgta
     1321 ctctccgttg aaaaagtctt ttgcaatgtc aacgcgcgtt atttttggac gaatcgcatt
     1381 tgtcagaaaa tcaaaaagcc ttgattccca tccaatattt gcagcattgc agcctaccgc
     1441 cgtcaattca accaacatcg tttcacgttg gccgccgtaa tggacgcgcc cgtattgggc
     1501 attttccgtt cccatcagcc agcagccttc atagaaacgc ccgcccgaat gcttggcttt
     1561 ttcaatgatt ccgaatccga aaatttcctc catcttggcg gaagccgcgc gtatgaaatc
     1621 gtcatcatcg aacaaatcaa acgaaagtcc ataaacatgg aaaaacgtgt cttcatgaat
     1681 tgaaaatgtg atttggtcaa tgaaagccga atctgataca ccgcgcctaa gaggaacgcc
     1741 taacaggttt cctttaccgt ccgttatgta cgtttcgtaa cattcgaaga cttcctgaac
     1801 cctgcccgcc gtttcggttt ctgtgccccc ccctgttaga taaggggggg gggaagattc
     1861 gaagcggttg ccggcttcct gccgtccgcc ggcgcctccg tcatcacgcc ggcaacctcc
     1921 tttgtcatcc cttgcttatc ttccatttgc gaatcctcaa aaacgggcaa aaaaaaagcc
     1981 ctgttacttg tagaaagtaa agggcgttaa tttttgttaa tcaccccttc ttagggacgc
     2041 aatatataag ggtcttaatt aaaggaagat gccgtctgat tttttcagac ggcatctttt
     2101 atttcctcga ccaaccgatt taatcgctcc ctacaaatag ctttattttt taattcgcac
     2161 tcccaaacga caatcacttt gaaaccaatc ttttccagct cggtttcatt tttaatatca
     2221 cgttcgcgat tttttgtgat tttttccaac caaaaatcca tattgctttt cgggatatgt
     2281 cctttattac aagaatgccc gtgccaaaaa cagccgtgta taaacacgac tgttttatat
     2341 ttaggcaaaa cgatatccgg tttgcctgca tagcgcttat cgtttttccg ataacggaaa
     2401 ccttgggaaa aaagaaattt ccttaccaat acttccggtt tggtttcttt gctatggatt
     2461 tttgacatca cgaaggaacg tttggatgga gtgaaaatat cggtcataaa atttaaggcg
     2521 atgtaggttt agctcgtttt tttgtttgtt ccacttttct caataaatat gaaactaagc
     2581 tgattaaatc caaagcatcc ttgtcattaa aaatactagg agataaattt gctcttaact
     2641 catgttgcac aggatttcta aatccagtcc atgttccgca tgcaagctgt tcaagaccat
     2701 cttctaaatt ttgttccgcc tgcgttttgt tattcgttaa tagaagcata gattttttac
     2761 tcccgaaggc tttttcaatc aattctcttc cgtctatgct atgaagacct gtctgcttct
     2821 gaaccttatc ttcgaaaact ttaactgcct cgattgcagc acttaaataa tccttctttt
     2881 cattgtaaaa atcgttacag gcagtatgca aatcctgatg caaatgccgc caatgatagt
     2941 aaggatattc tggaataata ctatgtaagg cagagattac ttccgcttgt tctttatttg
     3001 taagctccga atcttccaaa cgattcaaaa taacttctac cggttctctt tccttttctg
     3061 gcaatttgtt tttccaattt tcgatatcaa ctccgctttc acctttcact tcgtaatctt
     3121 tctctttttt tcttcttttt ctccaatctt gcccgatggc tcagacgatt tccccaagga
     3181 aaacccgtaa ttttgaagtt ttaggatgct tccaatccaa tgatgtccta tctgtagcga
     3241 ttaaatcttc accatcatta tctataaaat caatacttaa ccatcctgtc agataggatt
     3301 agaaatggct ggattcgcta tcagtgaaaa actcgggcat atttaccatt ctgccgttgg
     3361 caaacaaggt aatacctttc atattattct ttaaaggttt ttccgttgta atgaacttac
     3421 cttgtattaa tccgctgtat tcatactgtt cgaattttga agataacgat gaaatattgg
     3481 ttgctagatc ctggtattcc cattcgaatt gtggggtaac aagattatat tttaggtttc
     3541 cgtcaatgat tcttggttcc gagccgttca agcttacttt gattttaaaa tcctgagccg
     3601 gaaaatcaaa taaacgggaa agatgcccca cataattatc taacgggtat ccttgttttt
     3661 tcgtcagctc ggttaaagtt atagttgttc cgttttcggt attgggttta acagactctt
     3721 tctgaaactc cggttgataa atacgctcac tttttttaat ctctgcataa tccaaagtaa
     3781 aagtaacccg ttcgtttcct tggatagtag agatttcgat tttgttgcca agcctgaata
     3841 atgccaattt accaagacct tttttacccg ttggaattct tccgcacggg gaggcttgtt
     3901 tttcttccct tctgttccga ccgattctca aataaaaatc attgatttca tcgaagctca
     3961 ttcctatgcc attatcttta ataacgattt tatgctccgg tttatcgaat aacctaactt
     4021 ccacttccgt agcacatgca tcgtaggcat tcgctatcaa ctcggcaatc gcaggaacag
     4081 tgtgcgaata catctttaca cccaaatgtt cgattacggt tggctcgaaa ctcattttca
     4141 atttttcttc agacatgttt tctccttttc taacattcga tattatcaac aattgccctg
     4201 cctatttttt ccgcatacat cggaggaacg gcattaccaa tcaaacgggc tatcttgtcc
     4261 ctactgcccg ctttaaagac atagttacga gggaacgatt gcaatgttgc accttctcgc
     4321 agggataacg ccctatcttc ttcaggatgg gcaaaccgcc cgttggaaat gctaaagaat
     4381 ttggtcgtta tggtaggtgc gggcttatcc caccatagcc taccgaacgt gtctttaaaa
     4441 ctattgtctt ttccgataaa acattccagc tgtaattcgg gaatatgcgc aaaagccaaa
     4501 cggtttcctc cgtttttagg tatcaaagcc aatcgtttca aattgatatc ggataatccc
     4561 gcacaactat gcataaaatc cgtctcgtct tgatgtcctg ccataatttt gggaaagccg
     4621 ttttccattc ccaaaacatc gcgtaccgta agccgtttgc ccgaatactt gactggttcc
     4681 agcttttttt ggttattctg tttgcaatta acgtaaatct tttacggctt tggggaattc
     4741 cgtaatcagc cgtattgtga ataccgaaat ggacggtaaa tcctttttct tccaataact
     4801 ttatgaaatt atcaagcccg ctttctttca ttctgctcag aattcccggt acgttctcta
     4861 caacaacata tccgggattg aaatattcta caaaccgctg aaactccaaa agcaaactct
     4921 tggatttttc cgattttctt ttgtctgttt gaataacact ccaatattga caaggactac
     4981 aacctataag aatcaaatca tcatcattct tttttaagcc gagttctttt tccaaggttt
     5041 caggctgcaa ttcaaaaata tcagcctgta taaatttggc atttatattg gcttcatatg
     5101 tttctttgca gctaagttcg taatctatcc cggctaaaac ctgaataccg gcggattaca
     5161 ttccatagct cattccgcct ccgccacaga aaaaatcaac cgcctttaac ttactgtttt
     5221 tcatttctat ttaaataatt agactagtga gaactaactt tatataacat ataatttatt
     5281 gcttatcaaa aataatttga gattttatta aaattcatgc atctaaataa gcagaactta
     5341 atttcctcca aacgatcgga tatcttcaaa tgcacatttc acagaaaggt cgtctgaaac
     5401 ccattttcag acgacctttt tccacttcgc aaaacaagaa ttcccacccc tctccccaaa
     5461 catcgcccaa accagtaaat tccccatatt tcaacaactc cttggaagca aaccatgtcc
     5521 cgtatctacc tacccctcct atttccgccc cacatcgtcg aacgcggcct tttgtatttt
     5581 cagcaggaca aggttcgcga tgtccaaaaa atttccttag ggcgttatcg ggcgaaggta
     5641 ttcgattcgg aaaactatta ggcatggttg aagctggata gtgatttgta cattaaggac
     5701 ggggctgcga ttgcccttat gtctaaaggt gcaaacatgc cttaaattac taagcctata
     5761 acgaattgga aacaaaatgc cgtctgaaaa catcttcaga cggcattata aaatctgttc
     5821 accttttcag atgagtaatg tacaccccta tacaattttt gctaccatgc accataaatc
     5881 cacggctaaa gataattggg tttattcttt tatccagaaa caatccagat acggatcacg
     5941 ggtcataact ataggcttaa tattacacga ttctcattcc atcaaggcgg aaaaccgcac
     6001 aaatactgaa acactatcga tcgatttgta aacaagccta cttaagtaac ttgcagtcct
     6061 tatcatttcc tttaaaataa tccagcccgt cactacacga actggcggac ttcttgcaaa
     6121 taaaggttac tagattttca ttcatcttaa taataaaagg atttttatct ttatctatgg
     6181 ctaccgcctt caacatgaat ttactgtcta aagccccgcg cgcgattcca ttcaaacgga
     6241 tacaaaagcc ttctgcctct ttaatcggca aacttggcca tttggtagat gtttgtttaa
     6301 atctcccatt ctgcagataa aacttttcca taaaatgtgc attttctaac aaggctgccc
     6361 gcactgcatt tatctttgct ttctcaacat aattgcgata gctcggataa acaattaaag
     6421 caagtacaga caatatcaag accactgata ttaattcaac cagcgtaaac ccccgattat
     6481 cagtcattac tttacttcca ataagaacag attattcaac atatttcttt gaacagactt
     6541 actatcccat tcagcagtat gcatatttcc cac
//
LOCUS       DAWTCI010000099         6232 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWTCI010000099 DAWTCI010000000
VERSION     DAWTCI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.099"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(210..1595)
                     /locus_tag="WM481_001943"
                     /pseudo
     CDS             complement(210..1595)
                     /locus_tag="WM481_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            1713..1789
                     /locus_tag="WM481_001944"
     tRNA            1713..1789
                     /locus_tag="WM481_001944"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1747..1749,aa:Arg,seq:acg)
     gene            1807..1881
                     /locus_tag="WM481_001945"
     tRNA            1807..1881
                     /locus_tag="WM481_001945"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Glu,seq:ttc)
     gene            2044..2160
                     /locus_tag="WM481_001946"
                     /pseudo
     CDS             2044..2160
                     /locus_tag="WM481_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2318..3493
                     /locus_tag="WM481_001947"
     CDS             2318..3493
                     /locus_tag="WM481_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HFA9081188.1"
                     /translation="MQNIFDPLIIRGKSLIPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKNLGLEGEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLASAETEKVV
                     EFMSVAGLPARGIRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDQLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(3835..4383)
                     /locus_tag="WM481_001948"
     CDS             complement(3835..4383)
                     /locus_tag="WM481_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFA9081189.1"
                     /translation="MSQNNPIKPWYKHVWPWVLMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPANHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(4386..5882)
                     /gene="ccoG"
                     /locus_tag="WM481_001949"
     CDS             complement(4386..5882)
                     /gene="ccoG"
                     /locus_tag="WM481_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFA9081190.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKVKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPSGLI
                     RYTTESALEHEYAEKDIKKRLLRPRVAGYGAVLAVVVAAFLVGLSTRKMVEVDILKDR
                     GVMVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETI
                     TLPVQVSTIPEYADKGSHPIEFIFQYRESGASDGKPVVLEEDATFIGE"
BASE COUNT         1622 a         1690 c         1420 g         1500 t
ORIGIN      
        1 aaccaccctg cttgtccgat gcctcgggac ggacgggcaa accgacacgg cacgcaaacc
       61 gcgtcggcaa aaatgccgtc tgaaaaaatt cagacggcat cttcagacac attacctgca
      121 aacggcaaca cattccaatc caaaccgatc aggcaatcaa tacgaacggc tgtttacata
      181 cttaccagct ttcaccaacc ggtatcgatt taaccgattt ccttaatatt tttcctgtcc
      241 gttttaaact tcgccttaaa cacatccggt aaatctttat cgaaatacca aagcccgtca
      301 tccatttcca atgcgccgcc cattccatgc agaacgactt ttcccccgtt aaaggatcgg
      361 tttcggtaat ttccacctca tgcatttttc cccaatccaa aacaggcaac ttgtgtgcaa
      421 acgccaaaac ttgttcgtca aaacggccgc acgccttatc gcatcggcct gaaacatata
      481 cgggacaagg ctcatcctct gcataaccgt ccggaaggat accggctgag gcaggacaca
      541 atccgtatgt ttccgcccac gacgacaaag cccgtctcgc cgccttttta ttggcaaata
      601 atccggtagg cggattatcc gtcacaccgt ttttcaaagc cgctgttttc gcattcaaca
      661 tgccgtctga acctttttca aacctgacgg tcgtaaatgt tttaagcaga tttttggcag
      721 atacataaca acccgaatga taacgtccga ccaattccgc tttaatctta tatgcatgca
      781 ggctgcccaa tgcgggaaaa aaacggactt cctccgtatt gcaccaatca aacgggggct
      841 tttccggaat ccaacaaagc cgcaacctcg ccatatgccc gttcaaacgt accgatataa
      901 tttacttttc ccccgccgtc gaaacaggca agcacacctg taccgtcggg caaaccgtac
      961 agccgttccc tcaaccgttc gggaagcgcg gcaggaagaa gtttcggatt cgtcaaacgg
     1021 aaacactgcc tgctccactc ctcaagcccg tgtgccgaca ggctgtattc caaataatca
     1081 cacaacgccg atacatccgc catcgcacga tgcctgtctt ccacaacaat ccccaacctt
     1141 tcgatgatac cgtccaggct gtgcttgtaa aactgcggat acagccgacg cgaaagctgc
     1201 acactgcaca aagcaggcga tgaaaatccg atacccgcac gatgaaactc atgctttaaa
     1261 aacgtatagt cgaaacggct gttgtgcgcc accagcacgc accccttcaa taccgaaaac
     1321 aacccgccgg caatttcagc aaacaccggc gcgtcggcaa ccatgccgtc tgaaatcccc
     1381 gtcagttccg ccacaaactt cggaatcggt ttttgaggat tgaccagcca ttcatacctc
     1441 tccgcccttc cctgcccaaa cttgaccaaa gccacttcgg ttaccctgtc ttcatacaga
     1501 ttgccgcccg tcgattccaa atcaaccacg gcaacaggca ttccaaaccg taaaaacgcc
     1561 ttttccagca agggccagcg ggaaacaaca atcattttat tctctttaaa ttcaaacaac
     1621 aaaccgatat tttacacttt taaggcattt catccaacaa aataattgac agaatccgat
     1681 gattaccata aaattcaaaa atttattgca gcgcacccgt agctcagttg gatagagtat
     1741 ctggctacga accagagggt cgggcgttcg aatcgctccg ggtgcgccag taagaaaata
     1801 caatacgcgc ccatcgtcta gcggttagga catcgccctt tcacggcggt aaccggggtt
     1861 cgattccccg tgggcgtgcc agattccaaa tccccgaaat aatccctcgg ggatttttat
     1921 tgcctcggca attcgttacc atatctttac ctacccactt catcagaatc tcagacgtaa
     1981 tcgaatcata ttcaaacttt tgccatgcaa accgatatcc cataaccgga tgcggtgtcc
     2041 gttcaatata gtggattaaa tttaaatcag gacaatgcga cgaagcccca gacagtacaa
     2101 atagtacggc aaggcgaggc aacgccgtac cggtttaaat ttaatccact atattttgtc
     2161 cgattggtac agatataaca gcatattcca aagctccttc tgcttttctg tttaccactg
     2221 caatatttag agtataatac caaatttgag taatggttct aaaacagtta aaaccatttt
     2281 tcatgagcct gactgattcg tatattcgga gaaactgatg cagaatattt ttgacccttt
     2341 gattattcgt ggaaaatccc ttattcccat cgtgcaaggc ggtatggggg tcggtgtttc
     2401 cgcatcgggt ttatccagcg cggtggcgcg tgaaaacggc atcggaacga ttgccagtgt
     2461 agatttgcgc caccttcacg aagacctgct cgccgaatcc caaatcaatc cgagtgaaga
     2521 gaaatatacg tctttgaact gtaccgcatt agacagggaa atccaaaaag ccaaaagtgc
     2581 ttcagaggga aaaggactga ttgcggtcaa cgtgatgaag gcggtcaaag accacgccgc
     2641 gtatgtccgc caggcttgcg aatcaggggc ggatgcggtc gtgatgggtg ccggcctgcc
     2701 tttagacctg ccggaaatga ccgagggcta tcataaggat gtcgcgctgc tgccgattct
     2761 gtccgaatcg cgcggtatta atatcgtctt gaaacgttgg atgaaaaaag gcatattgcc
     2821 cgatgcgatt gtagtcgaac atcctgccca tgcggccgga catttgggtg catcaaccgt
     2881 tgaaggcgta aacgatgcca aattcgactt caaacgcgtg attgaggaaa cgtttgaagt
     2941 gtttaaaaat ttggggctgg agggagaaaa aatcccgctt attcttgcgg gaggcatggc
     3001 aaattttgaa aaagtcaaaa ccgccctaaa gaactgggga gcctccgccg ttcaaatcgg
     3061 tacggctttt gccgttaccg aagaaggaga tgcacacctc aactttaaaa aaacgctcgc
     3121 cagtgcggaa actgaaaaag tagtcgaatt tatgtctgtt gccggtttgc cggcgcgcgg
     3181 tatccgcacc aaattcctag acagctatat caggcgcgaa ggcaaacttc aggcaaacgc
     3241 caaagccgac ccgcgccgct gtacccaagg tttaaactgc ctgaccagct gcggtctgcg
     3301 cgacgggctt tccaaagcag ggcaattctg tattgatatc cagcttgctg ccgcattccg
     3361 tggagaagtt gataaggggt tgtttttcag gggaaaagac cagcttccct tcggtaatgc
     3421 catccgcacc gtccgcgaaa cgatacaata tctgctgacg gggagcgaac ctgttgcaac
     3481 gctcggacgc tgacatcagg taggatttga tttgcaaaca aaatgccgtc cgaagggctt
     3541 tcagacggcg tttttcaggt tgtgttccgg aacagtgtcg aaaaaacgga gagaaataaa
     3601 aacgttttta tgtgaacaaa atgccttccg cattatgtcg gacatacaac acaaatgccg
     3661 tctgaagcct ttcagacggc atttttcagg ctgcctttcg gaatgatgtt aaaaaataaa
     3721 cgggatgaga tacatttatt ttcgtccgac aaatcagacc atcacgccga ataatcaaac
     3781 aatgcctgcc cgacattgca tataacaaac caatgcaatg aaaacacggc ttttttattt
     3841 gcttcctgca ttattgaaaa gtttgtccat cggggtcaaa tcgaccgcat tgccctggct
     3901 ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc gcacatacca
     3961 gtggttggcc ggcggaaggg ttttgaacac cgcctcatat tccgccctgc cgttctgcgc
     4021 gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg ggtgcatcag
     4081 cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt ttgccgcatt
     4141 catatcggga gaaatgagga cctgcacccc gatatgccgt ctgacggctt cttcatcccg
     4201 atgaagctgg atgtcgatat gcttgccatc cttataataa tcgtccgtaa ccaaatctgt
     4261 cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa aaatcggccc
     4321 cgccatcaag acccacggcc agacgtgttt gtaccaaggc ttgattggat tgttttgaga
     4381 cacggttatt ctccgataaa ggttgcgtct tcttccaaaa caaccggctt gccgtctgat
     4441 gcaccgcttt cccggtattg gaagataaac tcgataggat gactgccttt gtccgcgtat
     4501 tccggaatgg tggacacttg gacgggaagg gttattgttt cgcgcggagc cacttttata
     4561 ccgccttcag gcagcccggt cagggcgatt tcgtcaaatc ctttgacact tgcggtaatc
     4621 agctgttctt tttcgctgtt gttgatgata cgcaggctat atgcgttttc cagccaacct
     4681 ttggcgtttt cgcgcaccat tacgccacgg tctttcagaa tatcgacttc gaccattttg
     4741 cgcgtggaca aaccgaccag gaaggcggcg acaaccactg ccaacaccgc gccgtaacct
     4801 gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cggcatattc gtgttccagc
     4861 gcgctttcgg tcgtatagcg tatcaaaccg ctcggataac ccattttgtc cataatctca
     4921 tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcaggcc gttgcggatg
     4981 tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc gcccaaaccc
     5041 gcctcttcct tgttgaccgt tttcttgcgg gcgccgcgcg gttcgccgcg ctccgcgtca
     5101 taagaaacaa tcagcgtgtc cttatcgaac atcgcgcttt ggaaacgtgc gtacggacac
     5161 atatgcaggc acactttttc gcgcataata tgggcgaaga agaaggtcat aaagccgtaa
     5221 aacgctgcgg caaacatcgc gccgccgcct gctgctccgg tgaataaatc gggaacaaac
     5281 tggcggatgg ggacaaacca gccggcaaac gtgatgcccg tccacgcgca gacaaggaaa
     5341 atcaacaggt atttggtggc tttgatgcgg attttggtga aattccacgg cgatttttcc
     5401 agcttcaggc gtttgtttct atcgccttcg accaggttgt cgatccacag cataatttcg
     5461 gtgtaaaccg tttgcgggca ggaatagccg caccacaaac ggccggcaat cgtcgtccac
     5521 caaaacagcc cgaaggcgca aatcatcagc agcagggcaa ggtaaatcaa atcgcccacc
     5581 cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac gacggcctgc
     5641 cttccgctcc aattgaacca cggaatgacg taaaacacaa actgggtcgc caatacggcg
     5701 gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg ttcgccttcg
     5761 ggatggattt gaatcacgct ggcacgcgga tcgaacgttt ttttcacttt cggcgcggct
     5821 tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt ttccgtggtc
     5881 attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc gttttgcttt
     5941 tcagacgtca tttttcttgt ttttttaagg cattgtgttt caagttttga gaaaatccgc
     6001 ttttcccaaa atatattccc gctattgtac aactttatgc gccgtccgga tgtattgggc
     6061 gaatacattt cccatccgca tcaaaacgcc ctgattttac cgcaccgccc gaacaaaatt
     6121 aaaatactct taaaaaacaa agactaaaaa aaacagacac cccttatcgg cagaaccgcc
     6181 ggcgcaagct cataaacaaa cgctatcaac aatccggcac acaatctata ac
//
LOCUS       DAWTCI010000100         6008 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWTCI010000100 DAWTCI010000000
VERSION     DAWTCI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.100"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(787..3540)
                     /gene="tbpA"
                     /locus_tag="WM481_001950"
     CDS             complement(787..3540)
                     /gene="tbpA"
                     /locus_tag="WM481_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent transferrin-binding protein TbpA"
                     /protein_id="HFA9081191.1"
                     /translation="MQQQHLFRFNILCLSLMTALPAYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIRTGRHAGEIRAHEAAGRGVQSFNRLAPVDDASTYAHFIVEAECENGSYETCKTRP
                     KKDVAGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDANQKQAGSLRGNGKYAGNHKYGGLFTSGENNAPVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDAWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGYDRFGS
                     NLRHQDYYYQSANRAYSLKTPAQNNGKKINPDPNGSEKNPYWVSIGRGNVVTRQICLF
                     GNNTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHR
                     TLSWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRSGDKIKAVKIDPEKSFNKEA
                     GIVFKGDFGNLEASWFDNAYRDLIVRGYEVQIKDGKEGVKGDPAYLNAQSARITGINI
                     LGKIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGSG
                     YDQPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYY
                     TVKKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLE
                     MKF"
     gene            complement(3627..5702)
                     /locus_tag="WM481_001951"
     CDS             complement(3627..5702)
                     /locus_tag="WM481_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFA9081192.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRAAPKY
                     QDVPSKKPEARKDQGGYGFAMRFKRRNWYRAANENEVKLKESDWEQTDDDEIKNPFKQ
                     KNIINALPGNEGELLQDSSQQGKGISKVRDHHDFKYVWSGFFYKRIKITTKKDESHKI
                     IEARSGPDGYIFYKGRNPSRKLPVSGEVTYKGTWDFLTDVKANQKFTDLGNASTKSGD
                     QYSAFSGELDYIVKKEEDKKEKHKGLGLTTEITVDFEKKTLIGKLIKNNMLINNNTKP
                     TTQYYSLEAQVTGNRFSGKAMATEKGENKQHPFVSDSSSLSGGFFGPQGEELGFRFLS
                     DDGKVAVVGSAKTKDETASSGGTSGGASVSTSNGAAGTSSENKLTTVLDAVELTPNGK
                     KIKDLDNFSNAAQLVVDGIMIPLLPTESGNGQADKGENGKTAFIYETTYTPESDKKDT
                     QTGMATNGVQTVSNTAGGTSGKTKTHYKVQACCSNLNYLKYGLLTRKNSESAMQAGES
                     SSRTAVQTAQGAQSMFLQGERTDEKEIPKDGNVVYLGTWYGHIAINGTSWTREASNQE
                     NGNRAKFDVNFKDKKITGTLTAANRQEATFTIDAMIEGNGFKGTAKTGDGGFAPDQNN
                     STGTHKVHIAEAKVQGGFYGPNAEELGGWFAYPGNGQAENAQTSSGNGNSAGSATVVF
                     GAKRQELVK"
BASE COUNT         1253 a         1580 c         1492 g         1683 t
ORIGIN      
        1 tttgcaactt caaatcaatc cgcaaactga aatcccgtca ttcccgcgca gtcgtgaatc
       61 cgaacgcgtc cgcacggaaa tccgcatccc gtcattccca cgaaagtggg aatctagaat
      121 gtcaaatctc aagaaaccgt tttatccgat aagttcctat gccgacggac ctggattccc
      181 gcctgagcgg gaatgacgga ggggaacggc gcacggacat ttgccggaat cttttatgtt
      241 tccattatca tactcttttc aatgcgctat tccaggaaaa agaaaatagc ttgtgcaggc
      301 tgctttttgt ttttcagacg acctgtaatt aacggctgct tatccaatct gcttttcaaa
      361 ctacctgcaa ccgcaaaatc tgctgcaacc cgcccctccc ctgtggggga gggtttggga
      421 gagggcattt ttcaagttgc ggcaattccc ccccgctcaa aaaattccct aaccgcccca
      481 atacaagcct tgcggcttac tgccctctct ctaaccctct cccacaggga gagaggacta
      541 tgatgcccgc cggcggcttg ctattaggaa tatctgaatc gtcattccca cgaaagtggg
      601 aatctagaaa ccccaacgca gcgagaatct atcggagaaa ccgaaacccg acagacccgg
      661 atgcacgcct gcgcgggaat ggcgggtttc gggattacgg tgtatcggga aggatgggaa
      721 acggtgggga ttgtgtaaaa aacgccgtct gaagcctttc agacggcatt tgcggcgttc
      781 ggacgtttag aacttcattt ccaagctaaa tgtgtagttg cggccggggg cggcatatcg
      841 gttgtaaacg ccgacatttt tgtgttggtt gactgcgccg ccggcagttt gccgcacatt
      901 ttcccaagta acatagcggt ggttgaggag gttgtacacg cccgcacgga gggtgaagtg
      961 ttttttaacc gtgtaataac cggacacatc cacaatatac caagggcggg tacggcgcgc
     1021 ggtggctttt gtattgcggc tgttgccgtt gagcaaagcc cggctgccca acaactctgt
     1081 gatttccttg gctttggaat aagtcagcat accgttcacg ccccatttgc cttccggttg
     1141 gtcatagccc gagccgacga catagcgcga gggttggatg gcatcaaaca gatgtgattg
     1201 aatatcggtg cggtctgcgc gttttttaat atcgcggaca cggacacgat tataggcaaa
     1261 tgtagaatac caaccttcgg gcaatttatc ccatacgccg ttccaatcga ttttgcccaa
     1321 aatattgatg ccggtaatcc gcgcgctttg ggcattgagg taagccgggt cgcctttgac
     1381 tccttctttg ccgtctttaa tttgcacttc ataaccccgg acaatcaaat cgcggtaggc
     1441 attgtcgaac caacttgcct ccaagttgcc gaaatcgcct ttaaacacga tgccggcttc
     1501 tttgttgaac gatttttccg gatcgatttt gacggctttt attttatcgc ccgaccgcca
     1561 gccgtacatt tccgcaaacg agggcaggcg gaagccggtt gaagtgcggt aagtcaaatc
     1621 cagccagtcg gcaggtttga ggacgatgcc ggcgttccag gacagggtgc ggtgcgtgcc
     1681 ggtggaaacg ctgccgtcgt ccgaatgcgt gctgcggtag tcgtagcgca agcccgcgcc
     1741 gacatccgcc cacctgccca aacggacatt gtcccggacc gccgcgtaat agcttttgcc
     1801 gttgatgctg cgcggcgtgc agtccgtata agtattgttg ccaaagaggc agatttgcct
     1861 cgtaacgaca tttcccctgc ctatgctgac ccaataggga ttcttttcgc tgccgttggg
     1921 gtcggggttg atttttttgc cgttgttttg agcgggcgtt ttcaacgaat aggcgcggtt
     1981 ggcactttga taataataat cctgatggcg cagattagag ccgaagcggt cgtaaccgag
     2041 attcacgctc aggttgtggc ggattttggc ggtatcgaag gattttttga atgccgcctg
     2101 caagagcctg tggctttccc cgtaaatcac gcggtcggat ttgtaatagg aaaacggctt
     2161 gtcggcactc ggacggcaat atttgtccga accgtcggca gaacagtgcg tctgctgaaa
     2221 atggttgtcc aaaccgatgc cctgacggtc gtaagagagg cgggcataat ccgcccaagc
     2281 gtccttatcg gcattggtat agacatattc caaaccgtag cggcttttgg tgtgcgtctc
     2341 gtcgtaaaac acgcccgtac cgtattccgc gcccaccggc gcattgtttt cgccgctggt
     2401 aaacagtccg ccgtatttgt ggttgcccgc gtatttgccg ttgccgcgca aagaacccgc
     2461 ctgtttttga tttgcatcaa aaaccgcctt ggtcaggaat gccggaaccg tcatatcgcg
     2521 cgtgtcgaaa gtttgttgcg tgcgttcgag tatgccgccg atgtagtgcc gtttgttttc
     2581 aaaacgaaaa cccgggcgga acagccacga ccggctttcg tatgaaagcg gatcggcgag
     2641 gaagcggttg gggcccgtgt agtctcgggt agaaaccgtt tgacgttcgt ctttgccggc
     2701 aacatctttt ttcggtctcg ttttacacgt ttcataactc ccgttttcgc attctgcttc
     2761 aacgatgaaa tgggcgtatg tgctcgcgtc atcaaccggc gccagcctgt tgaagctctg
     2821 aacgccgcgt ccggcggctt cgtgggcgcg gatttccccc gcgtgccggc cggtgcggat
     2881 cagcaaagcc tccgcaccgc cgatgcgccc cgccagcgcg atggattggg taagcccccg
     2941 gtttttgccg gaataggcgg ttttactctg aatgccccac tgcctgcctt ccccgataac
     3001 atcgtcggcg gttttggttt gaaatgcgac cgagcccgcc aatgcgccgc tgccttgttc
     3061 gaccgagttt gagcctttgc tgatttcgac agccttgacg ttttcatact cgatttcatt
     3121 gattgcgccg ctgctgcccg ccgtcctcgt cccgcccaat gccgcctgcg cggtgtagga
     3181 ctgtatttgc gccaagccgt ccaccgtcaa ggagacgcgg tttttgtcca taccgcgtat
     3241 cgagtagccc gagcttgcgc cgcgcccctg ttcgacgacg gcaatgccgg ggtcgtaacg
     3301 cgtcaggtct cggatgtcga gtacctgttc tttgctgagg gtgtcggcgg ttttgaccaa
     3361 tttgcccaaa ccggttactt cgttatcgcg gcgggttttc tgttttttgg cttttacctg
     3421 tatggtatcc aactgttttt cctgtgcttg tccggcttgc acattttctg cataagcggg
     3481 cagcgcagtc attaaagaca ggcataaaat attgaatcgg aacaaatgtt gctgttgcat
     3541 agtgtttccc taatcttcgc tttcagacgg aatcggaagg aacgatgccg tccgaagcct
     3601 tattctcgat tgttcggcag ccgtgcttat ttcacaagct cttggcgttt cgcaccgaat
     3661 accacggtcg cgctgcctgc tgaatttcca ttgccggatg aagtttgcgc attttccgct
     3721 tgcccattgc ccggataggc aaaccatccg cccaactctt cggcgttagg cccgtaaaaa
     3781 ccgccctgca ccttggcttc tgcgatgtgc actttatgtg taccggtgct attgttttga
     3841 tccggcgcaa agccgccatc accggttttc gccgtacctt taaagccgtt gccctcaatc
     3901 atggcatcaa tggtaaaggt tgcctcctgc ctgtttgcag cggttaacgt gccggtaatt
     3961 tttttgtctt tgaaattcac gtcaaacttt gcccgattac cattttcctg attggaggct
     4021 tcgcgggtcc aacttgtgcc gttgatggca atatgcccgt accaagtgcc tagataaacg
     4081 acgtttccgt cttttggaat ttctttttca tcggtgcgct cgccttgaag gaacatactt
     4141 tgtgcgccct gtgccgtttg gacggcagtt cggctactgc tttcgcctgc ctgcatcgcg
     4201 gattcgctgt ttttgcgcgt cagcagcccg tatttcagat aattgaggtt ggaacagcag
     4261 gcttggactt tataatgggt ttttgttttg ccacttgtgc cgcccgccgt atttgaaacg
     4321 gtttgcacgc cattggtcgc catacctgtt tgggtgtctt ttttatcact ttccggcgtg
     4381 taggttgttt cgtagataaa ggctgttttg ccgttttcac ctttatctgc ctgaccgttc
     4441 ccgctttcgg tgggcaggag cggaatcata atgccgtcga caaccagttg ggcggcgttg
     4501 ctgaagttgt cgagatcttt gattttcttg ccgtttggtg tcaattcaac cgcatccaaa
     4561 accgtggtca gcttgttttc agacgacgtg cctgccgcac cgtttgatgt ggaaacgctt
     4621 gcaccgcccg aagtgccgcc actgcttgcg gtttcgtctt tggttttcgc gctgccgaca
     4681 acggcaactt ttccatcgtc gctcaaaaag cggaaaccca attcctcacc ctgcgggccg
     4741 aaaaagccgc cgctcagaga agacgagtcg gaaacaaagg gatgttgttt gttttcgcct
     4801 ttttcggttg ccatcgcctt gccgctgaag cggttgcctg ttacttgagc ctcaaggctg
     4861 taatattggg tggtgggttt agtgttatta ttgattaaca tgttgttttt aattaatttt
     4921 ccgatcaggg tttttttctc aaaatcaacc gttatttccg ttgttaatcc caaacctttg
     4981 tgcttttctt ttttatcctc ctcttttttg actatataat ccaactcccc ggaaaaagca
     5041 ctatattggt ctccggattt cgtagaagca tttcctaaat ctgtaaattt ctgatttgct
     5101 ttcacatcag ttaaaaaatc ccaagtacct ttgtacgtaa cctcccctga aacaggaagt
     5161 tttctcgagg gatttctgcc tttataaaaa atataaccgt caggaccgct tctggcttcg
     5221 attattttat gagattcgtc ttttttagtt gtaatcttaa tccgtttata aaaaaaaccc
     5281 gaccatacgt atttaaaatc gtgatggtcc ctaaccttag atataccctt accttgttga
     5341 ctggaatctt gcaataattc cccctcattt ccaggtaagg cattaataat atttttttgt
     5401 ttgaaagggt ttttgatctc atcatcatcc gtttgttccc aatcactctc tttcagttta
     5461 acctcgtttt cgtttgccgc ccgataccaa ttccgccgct tgaagcgcat cgcgaaaccg
     5521 tatccgcctt ggtcttttcg ggcttccggt tttttggaag gaacatcttg atactttggc
     5581 gcggcacgcg gggcttcggt atcgacagaa tcaagatcga aactgccgcc tccgcccaga
     5641 caagcgctca acaaaaacac gggcagcacc atagcagcct gattcaccaa tggattgttc
     5701 ataacaaacc cgattcaatt aaagaatgat aaggattatt attttattta tttttaaaaa
     5761 atttgcaaat gcttttttat atttttagta aataaaccaa aataccgtca ttcccgcgct
     5821 gcttttaaaa ccgcctgcaa ccgccaaacc tgctgcaatc cgctccctcc cctgcgcggg
     5881 ggagggctgg ggagagggca ctttccaagt tgcggcaacc tctcccaaat cccttagtct
     5941 tctaaaacac aagccttgcg gcttactgcc ctctctctag ctttctcccg cagggagaga
     6001 ggacgggg
//
LOCUS       DAWTCI010000101         5747 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWTCI010000101 DAWTCI010000000
VERSION     DAWTCI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.101"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            5..1925
                     /locus_tag="WM481_001952"
                     /pseudo
     CDS             5..1925
                     /locus_tag="WM481_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1982..2959
                     /locus_tag="WM481_001953"
     CDS             1982..2959
                     /locus_tag="WM481_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFA9081193.1"
                     /translation="MDMKRRDFLKMTAALAAAGVSPSLLTAGKEQFTVYGAPAMPSVT
                     IAVTALQGKLAKQADVSLKIWRSPDQLRAGVASGQFKVMMSPSNVGVNLRNQGQKVGM
                     VNILTNGITQLVCKGSAIASPQDLVGKKILVPFKNDMPDIVLQALLKKLKIDAHKVSI
                     TYAATPPEAVGLFPSKGYHAVILPEPMATASLLKGKTIGINVVHGFDLVKAWGQAFGT
                     KPLIPMAGIIANEEYFHAHKAQFDIFHQDLKNALNWILANRQNAAKIGKNYLPAPEPA
                     LVMGLDGARLTVSKGSEVKNEILKFYEILMQFNPELLGGKLPDNGFFLA"
     gene            3296..3433
                     /locus_tag="WM481_001954"
     CDS             3296..3433
                     /locus_tag="WM481_001954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081194.1"
                     /translation="MNEDKYRENMTWDEIENVVGTLVSEERMLKSNSFLSIKNSKNRL
                     A"
     gene            3512..4281
                     /locus_tag="WM481_001955"
                     /pseudo
     CDS             3512..4281
                     /locus_tag="WM481_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776012.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            4308..5000
                     /locus_tag="WM481_001956"
     CDS             4308..5000
                     /locus_tag="WM481_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFA9081195.1"
                     /translation="MICLENVRFEILRDPIVRDFSLNLQQGEVKALFGPSGCGKTTVL
                     RLIAGLETPKSGTIRNTFHKTGFLFQENRLPENLTAMQNIAIFMDNPDEGEIVALAAK
                     VGLTAGDLNKYPTELSGGMAKRVAFLRLLLCGCDLALLDEPFVGLDRDLRDILVAMLV
                     EKIERQGMACILVTHDRFEAARLSHEIMLLSAKGMNVQNVITLPTPLSERDSAFEEVV
                     VAREFQGIHYYE"
     gene            5192..>5747
                     /locus_tag="WM481_001957"
     CDS             5192..>5747
                     /locus_tag="WM481_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFA9081196.1"
                     /translation="MFSTVITAAVLYIATAVDLLVILLIFFARANTRKEYRDIYIGQY
                     LGSVILILVSLFLAFVLNYVPEKWVLGLLGLIPIYLGIKVAIYDDCEGEKRAKKELDE
                     KGLSKLVGIVALVTVASCGADNIGLFVPYFVTLDLVDLLVTLLVFLILIFVLVYTAQR
                     LANISGVGEIVEKFSRWIMAVIYIG"
BASE COUNT         1513 a         1367 c         1483 g         1384 t
ORIGIN      
        1 agaaatgaaa ctaaatactc tcacatgggc tttgatgacc gttttttccg ttgcgccatc
       61 ttgggcagaa caaccggcaa atactgaaga aatacaaccc gtcaaaacct tctccccgcc
      121 caaaccgatt gcaccgaccg ccgcacaagg ctatttcccc gaaaaccaat tcgaccgctc
      181 cgaccgcagc gattattact ttgttaccga aaacatagac caagccttcc gtccgctgaa
      241 ggcaaacagc agtttttacg gcaaaagctt ttacaactcc gttaccgcgc aagcactcgg
      301 ggcgaaggta tacggcgtag ccaacctcaa tcgcaccaag gcaaacggct acaaagatgg
      361 cggtgggcgc gacactgatt ggaaatacag ccgcttcaat caggctttgg tactcggttt
      421 cgtgccgtct gaaaatcaag aataccgcct cacttatctg cacgacgaca tcaacaacga
      481 ccgccagccg caggtcgtca acgacgcatt ggacaccgaa cgccacatct ccaaactcaa
      541 cgtgcgttgg ggcaatgccg atttgagcaa tacggtcagc gcggaagcag gcgtcatcaa
      601 actcaaacgc catgccgaca attactcctt gcgaccaaac aacacgccgc agcaagtgtt
      661 cgtagaactt gaccgcaaag tgtatgattt ctcgctcaaa cacgatgcgg atttcggcaa
      721 attccacaac acggcagccg tcagctaccg caacgacagc caaaacggcg aacgcaacac
      781 ccacactgcc atgtgcgatt tcctcaacgg ctaccgtttc gccgatgtgc acatcgaccg
      841 ctggtgcatt gccgatacct tatcttacaa gtttgacgac cgacacaaac tgggcttggg
      901 cttaagctac gaactcaacg aagcggacat tcgcaaaaat acggcacagc ctgaaaaccc
      961 aataaaaccc ggtttcccct tcgcctcatc acaacaaatc tggaaaaccc attacggcta
     1021 cgacttcaac ggcaaagtgc gccgtcatgc tttgtcaggc gaactcaaat acgatttcac
     1081 gccgtctgaa acgcaaaaat acagcgtttc cctcgcgcat ttggaacgca tcggcgacaa
     1141 caccgaacgc ttcaactcgc ttgctgccat cgtgcaaaac cgtatgagcg gcatgctgat
     1201 gaaccaaaat ccagccgccg ccattgcagg caatccccct gctgaaaact gaaaaacaca
     1261 accgcatcag gctcaccgcc gacagccgca acgactacta caacggctac atgaactcgc
     1321 tcgcaggcgc gggctggaac gtgggcggca cgcttgtggc ggacaaagtc aaagacctga
     1381 ttatttttga ccgcgcacac ggacaaagcg gcacagcttc caaagacggc ggcatcatca
     1441 cacgcaacgt ggacgcacga ttgtttaccg cgcaggccta cgcgcgttac aacttcaatc
     1501 cgcattgggc ggcaggcatc aaagccgcct acaactacgg acacaacgaa accgacggca
     1561 ggccgcccta tcaaatccgt ccgtttgaag ccgccgtcca agccgactac aaaaactact
     1621 ttgcccacgg cagctacaac atcggcgcgg caacacgctt tgtcgccaaa caaacgcgcg
     1681 gtgattttga tatggcaagc ggtctgggca tagacaaacg cgaagccgcc aaaggcttta
     1741 ccgttgccga cgtgtacgca ggcgtaaaca tcaaagacaa atacggcttg cgcttgggcg
     1801 tgaacaacgt attcaacaaa aaatacgtcg aatacatcag cggcgaccac gtcctcgccc
     1861 tatcccccag cgtagtgtat gcaccgggca ggacatattg gttgagtttg catgcggcat
     1921 tttaaaacca tgccgtctga aaaatacctg cattggctta ttaagaatta ggagataaaa
     1981 aatggatatg aaaagacgcg atttcttaaa aatgaccgcc gcgctggcag ccgcaggcgt
     2041 ttcgccttct ctgcttactg ccggtaaaga gcaatttacc gtgtacggcg caccggcaat
     2101 gcccagcgtt accattgccg taacggcgtt gcaaggtaaa ctggcgaaac aggcggacgt
     2161 gtcgctgaaa atctggcgtt cgcccgacca actgcgcgcg ggcgtggcaa gcgggcaatt
     2221 taaagtgatg atgagtccga gcaatgtcgg cgtaaacctg cgcaaccaag ggcagaaagt
     2281 cggcatggtg aatattttga ccaacggcat cacgcagttg gtctgcaaag gcagcgcgat
     2341 tgcctcgccg caggatttgg tcggcaaaaa aatcctcgtg ccgtttaaaa acgatatgcc
     2401 cgacatcgtg ctgcaagcct tgttgaaaaa actgaaaatc gacgcacaca aagtcagcat
     2461 tacctacgct gccacaccgc ccgaagcagt cggactattt ccaagtaaag gttaccatgc
     2521 cgtcatcctg cccgaaccga tggcaaccgc cagcctactg aaaggcaaaa ccataggtat
     2581 aaacgtcgtg cacggttttg atttggtaaa agcatggggg caggcgtttg gcaccaaacc
     2641 gctgattccg atggcaggta tcatcgccaa cgaagaatat ttccacgcac acaaggcgca
     2701 gttcgacatc ttccatcaag atttgaaaaa cgcgctcaac tggatattgg ccaaccgcca
     2761 aaacgcggcg aaaatcggca aaaactacct acccgccccc gaacccgccc tagtcatggg
     2821 cttggacggc gcacggctga cggtaagcaa aggcagcgaa gtgaaaaacg agattttgaa
     2881 gttttacgaa atcctgatgc agttcaaccc ggaacttttg ggcggcaagc tgccggataa
     2941 cgggttcttc ttggcttaaa gggaaagagg ccgtctgaaa agggtagggt ggttgtgaat
     3001 acctgattta tcatttttca gacggcctat taaggcattg tcagcggcgt gggtcggatt
     3061 cttgaatccg acatttcggc taaacggggc tgtcggatac aagtatccga cctactcttt
     3121 tcagacggcc tatcagataa tagttcgaac agtacacaaa gagccgtcgg attgcctgaa
     3181 atgttgggtc atgacccaac ctacgcttac tggggaaacg ggatcgaagt gtgaggtatg
     3241 tggcggggtt gtttaataat gaaattttat aactatttga tagggtggta atgccatgaa
     3301 tgaagataaa tatagggaaa atatgacatg ggatgaaatt gaaaatgtag taggaacttt
     3361 agtttctgaa gaaagaatgc ttaaatccaa tagcttttta agtattaaaa atagtaagaa
     3421 taggttggct taatcacaac cataacccaa cgaaaatact aaaattccaa gctaaggcta
     3481 tcctttccaa accatcccca tcaaccaaac catgattaaa accgacaaaa tccgcaaacc
     3541 gcagcctgcg ctgttttaca tcatcgacta cctttggagc ggctttgccg gtctgagcgt
     3601 ggcgatggtg gtggtggcgt tgtgggcgtg gggcagcgcc gtgttcggcg agtttatgct
     3661 gcctgcgccg gtagaggtgt ttcaaaaatc tttggattta ttgaaacatt ttcaggaaaa
     3721 tgaaatcggg atttcgctgt ggcggtcggt agtgggtatt tcggttgctt tgatagcagg
     3781 attggcggcg gggctggtgg caggcagttt taagacggcg atggcgttgc ttaagcctgt
     3841 gattacgatt ttgttagcaa tgccgccgat tatttgggtg gtgatggcgt tgttttggtt
     3901 cgggttcggc aatccgagcg tgttgtttac catcattgtg ttggttgcgc cgctgacgtt
     3961 tgcgagtgcg gcggtcggaa tggcgagcgt gaacaagcag catgaggagt tgtttgacgc
     4021 ttataaatta ggccgtctga aaaaaatccg ctatctgtat atcccgcatc tgacgggcta
     4081 tgtgatttcc agcgtcggcg tggcggtggc gatgggggtg aaggcggtga tcatggcgga
     4141 actcttaggc gcgagcaaag gcgtgggcgc gcggattgcg gacgcgaggg caatgctgta
     4201 gacttcgacg gtaatggctt atgtggtgtt ggtcatggta tttgtgtcgc tgtttgaata
     4261 cctgattcca agcctttgta aattttgttt atgccgtgga ggagatgatg atctgtcttg
     4321 aaaacgtgcg ttttgaaatt ctccgcgacc ccatcgtgcg cgatttcagt ttgaacctgc
     4381 aacagggcga agtcaaagcc ttgttcgggc cgagcggctg tggcaagacg acggttttac
     4441 ggctgattgc gggcttggaa acgccgaaat cgggcacgat acgtaatact ttccacaaaa
     4501 cgggttttct gtttcaggaa aaccgcctgc cggaaaacct gaccgcgatg cagaatatcg
     4561 cgatttttat ggacaaccca gacgaaggcg aaatcgtcgc gctggcggcg aaagtcgggc
     4621 tgactgcggg cgatttgaac aaatatccga ccgaattgtc cggcggcatg gcgaaacggg
     4681 tggcgttttt gcgcctgctg ctgtgcggct gcgacctagc cttgctggac gaaccgtttg
     4741 tcggtttgga ccgcgatttg cgcgatattt tggttgctat gctggtggaa aaaatcgagc
     4801 ggcagggcat ggcgtgtata ctggtaacgc acgaccgctt cgaagccgcg cgcctgagcc
     4861 atgaaattat gctgctttcc gctaagggca tgaacgtgca aaacgtgata accctgccca
     4921 cgccgctgtc cgaacgcgat tcggcttttg aagaagtcgt ggtggcaagg gagtttcagg
     4981 ggattcatta ttatgagtga tgaggggatt tgagtgtctg acaaacctta cggttcgttg
     5041 ccctctccct aaccctctcc cacggggaga gggttagcaa gcctcaggct tgcctcttta
     5101 gcgaaagata cgaatctgtt atccgaacgc gattcggttt ttgaaaaagc cgtggtgcca
     5161 agggggcagg ggattcatta tgaggtgctt tatgttttcg actgtgatta ctgctgctgt
     5221 tttatatatt gctacagcag tagatttgtt ggtaatacta ttaatatttt ttgctagagc
     5281 aaatactaga aaagaatatc gagatattta tatcggacaa tatttaggtt ctgtaatttt
     5341 aatattagtt agtttatttc tagcttttgt tttgaattat gttccggaaa aatgggtgtt
     5401 gggtttatta ggtttaatac cgatttactt aggtattaaa gttgctattt acgacgattg
     5461 tgagggcgaa aaaagagcta aaaaagaatt ggatgaaaaa gggttgtcaa aattagtcgg
     5521 tattgttgct ttggttacag ttgctagttg tggtgcagat aatattggac tttttgttcc
     5581 ttactttgtg actttagatc ttgtcgactt attagttact cttcttgtat ttttaatatt
     5641 gatttttgtt ttagtatata cagcacaaag attggctaat atttcaggtg ttggtgaaat
     5701 tgtagagaag tttagtcgtt ggataatggc tgttatttat attggtt
//
LOCUS       DAWTCI010000102         5710 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWTCI010000102 DAWTCI010000000
VERSION     DAWTCI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.102"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(363..518)
                     /locus_tag="WM481_001958"
     CDS             complement(363..518)
                     /locus_tag="WM481_001958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081197.1"
                     /translation="MPSESPSDGIGFPKHSAPLSSCRKQTPIRFAANAQKTQCRLKRQ
                     TGFRRHR"
     gene            complement(535..2331)
                     /locus_tag="WM481_001959"
     CDS             complement(535..2331)
                     /locus_tag="WM481_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFA9081198.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLAAKNIRIQHPDDLLDQVWTSRPAIPAETVFIH
                     DHAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDSAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESARLIEGINPSTFFKSVKSEADIARIREAMEHDGAALCGFFAEFEDIIGKGG
                     SLTEIDVDTMLYRHRSARPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVADPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIEWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2789..4042
                     /locus_tag="WM481_001960"
     CDS             2789..4042
                     /locus_tag="WM481_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFA9081199.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            4197..5708
                     /gene="lysS"
                     /locus_tag="WM481_001961"
     CDS             4197..5708
                     /gene="lysS"
                     /locus_tag="WM481_001961"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFA9081200.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLQAQYGEIGKEELDPQAVPVKIAGRMMLKRQMGKASFATIQDVTGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDLQTIRDVILFPQMRPE"
BASE COUNT         1201 a         1489 c         1678 g         1342 t
ORIGIN      
        1 cctccccggc cctcccccac gggggaggga gcagattgcg gcagatttgg cggttgcagg
       61 cggtttgatt tgcgagggaa tcggacgcgc aggcgggaat ccggatttgt ccgcgcggaa
      121 acttatattt cgtcattcca ccaatcccgc ccacagccca cttttccgcg tcattcccgc
      181 gcaggcggga atccagtccg ttcggtttcg gttatttccg ataaattcct gctgcttttc
      241 attcctagat tcccactttc gcgggaatga cgggatttca gggtaaagat ggcgggagcc
      301 ggagggctgt gaagagctgt tttcgcagga atgacgatat gcggatttgc tgttttcatc
      361 cgctaccgat gccgtctgaa gcctgtttgg cgtttcagac ggcattgggt tttttgtgca
      421 tttgccgcaa aacggatcgg tgtctgcttg cggcaagacg ataacggtgc ggaatgtttt
      481 gggaaaccaa tgccgtctga agggctttca gacggcattt ttgtaccgtg ccgtttaacg
      541 cgccagcggt tcggtgcgtt tgatcagcca cgcttttgcc gcgccttcgg tcagcggctc
      601 gaggcggcgg cggacttcgg cgtggtagcg gttgacccat tcgatttcgc cgtcggtcat
      661 cagggcggtg tccatcaggc gtgtatcgat ggggcagagg gtcagggttt caaaacagag
      721 gaagctgccg aactcggttt cctgcgggtc ggcgacggct tggttggcgg cgaggttttc
      781 aatgcggatg ccccattttc ccggacggta gaggccgggt tcgatggagg taaccatgcc
      841 ttttttcatg gcggtttcgg gcgtggcggg ggcggcgaag gcgatgcgct gcgggccttc
      901 gtggacgttg aggaaatagc ctacgccgtg gccggtgccg tgtccgtagt cgcattgcgc
      961 ctgccacagg ggtttgcggc aaatcgcgtc aatcagcggc gaggggatgt tttcggggaa
     1021 cacggcttcg gcaagcgcga tatgggcttt gagaacgagc gtgttgtcgc gtttttgttc
     1081 tgctgtgggc gtgccgacgg ggacgacgcg ggtgatgtcg gtcgtgccgc ctttgtattg
     1141 cgcgccggag tcgatgagca gcagaccgtt gccgctgatg gtgctgtggc tttcgggtgt
     1201 tgcgctgtaa tgcggcaggg cgccgttggc gttgaagcct gcgatggtgt cgaaactcaa
     1261 ggacacaaaa cccgggcgcg cgctgcggtg gcggtaaagc atcgtatcca cgtcgatttc
     1321 ggtcaggctg ccgcctttgc cgatgatgtc ttcaaactcg gcgaagaaac cgcacaatgc
     1381 cgcgccgtcg tgttccatcg cttcgcggat gcgggcgatg tcggcttcgg atttgacgga
     1441 tttgaagaag gtggacgggt tgatgccttc aatcaggcgc gcgctttcgg gcaggcgcac
     1501 gagcgtactg acggcggttt tgttcggctc gatgagcagc gcgccgccga tttgcgcgag
     1561 tttgtcggca acttgggcgt aaggctcgac cgtgatacct gcggtttgca gcgcggcggc
     1621 ggcttcggcg ttcagacggc attgttcggt aaacaggacg gcactgtctt tgccgatcag
     1681 caggaaggac acgaaaacag ggttgaaagg cacgtcgctg ccgcgcaggt tggtcagcca
     1741 agcgatgtcg tcaagcgagg aaaccaagtg gtaatccgcg cctttttccg ccataactgc
     1801 gcgcacgcgg gcgagtttct cggcggcggt ttcggagacg taggcgtggt cgtggatgaa
     1861 cacggtttcg gcggggatag cgggacggct tgtccacact tggtcaagca ggtcgtcggg
     1921 gtgttggatg cggatgtttt tggcggcgag cgattgcgcc aaagtgcgtt tgccggtgag
     1981 cgacaccata tcggaaggga tgccgacggc agcgttttcg ggcaggtttg ccgcgagcca
     2041 ttcgttgtac ggcggcactt gcccgctttt ttgcagttcg atgccgctgc ccgcaagctg
     2101 cttggcggct tgttcccaat agcggctgtc cacccatacg cccgcttcgt cggcggtaac
     2161 gacgaatgtg ccgaccgagc cggtaaagcc cgacaattcg cgccgcgcct gccaatgttc
     2221 gggcaggtat tcggacaggt gggggtcggc ggaagggatg acgagtgcat ccaagccttg
     2281 cgccttcatg gcttcgcgca atgcggacag gtaattcgat acggtattca taataattcc
     2341 tccaatcggg ttttgcggct tcagacggca tggacggaaa tctgaaatgc cgtctgaaaa
     2401 acataaccat cttatcaaat cgccagcctg ctgcaaaacg gacgggctga tcggatggtg
     2461 cgtgtttgcg cgaaaaaagc gatgtaaggt tgtttgttgt aaaattttag gcataaaatg
     2521 tcgtttcatg cctttttttg gaagtaatac ggaaaatata gtcaagtttg agcatgaagt
     2581 tctaatagtg tgtgttggca ggtatttgga ttacgttaca atacgcgctt tcatcaaaat
     2641 ttccctaccg ggcggtatcc tgtttttgaa gacggtagga tttgggtttg tttgggggca
     2701 atcccgccca aattggagca atagataggg ctgtgtccga atatgcggct ctgtgtgttg
     2761 aaacacggtt aaaaatacgg aagactttat gtccgaacaa catatttcga cttggaaaag
     2821 taaaatcaac gcattgggac cggggattat gatggcttcg gcggcggtcg gcggttcgca
     2881 cctgattgcc tcgacgcagg cgggcgcgct ttacggctgg cagatcgcgc tcatcatcat
     2941 cctgaccaac ctcttcaaat acccgttttt ccgcttcagc gcgcattaca cgctggacac
     3001 gggcaagagc ctgattgaag gttatgccga gaaaagccgc gtttatttgt gggtattcct
     3061 gattttgtgc atcctctccg ccacgattaa cgcgggcgcg gtcgccattg tcaccgccgc
     3121 catcgtcaaa atggcgattc cctcgctgat gtttgatgcc ggcacggttg ccgccttgat
     3181 tatggcatcc tgcctgatta ttttggtgag cggacgttac cgcgctttgg atcgcgtttc
     3241 caaaatcatc atcgttactt tgagtatcgc cacgcttgcc gccgccggca tcgctatgtc
     3301 gcgcggtatg cagatgcagc cggactttat cgagccgaca ccgtggacgc ttgccggttt
     3361 gggcttcctg atcgcgctga tgggctggat gcctgcgccg attgaaattt ccgccatcaa
     3421 ttctttgtgg gtaaccgaaa aacaacgcat caatccttcc gaataccgcg acgggatttt
     3481 tgatttcaac gtcggttata tcgccagtgc ggttttggct ttggttttcc ttgcactggg
     3541 cgcgtttgtg caatacggca acggcgaagc agtgcagatg gcgggcggca aatatatcgg
     3601 gcaattgatc aatatgtacg ccgttaccat cggcggctgg tcgcgcccgc tggtggcgtt
     3661 tatcgcgttt gcctgcatgt acggcacgac gattaccgtt gtggacggct atgcccgtgc
     3721 cattgccgaa cccgtgcgcc tgctgcgcgg aaaagacaaa acgggcaacg ccgaattctt
     3781 tgcctggaat atttgggtgg cgggcagcgg tttggcggtg attttctggt ttgacggcgt
     3841 aatggcgaat ctgctcaaat ttgcgatgat tgccgctttt gtgtccgccc ccgtgtttgc
     3901 ctggctgaat taccgtttgg tcaaaggtga tgaaaaacac aaactcacat caggtatgaa
     3961 tgcccttgca ttggcaggct tgatttatct gaccggtttt accgttttgt tcctattgaa
     4021 tttggcggga atgttcaaat gataacgatg ccgtctgaaa ccgcaaaccg ctttcagacg
     4081 gcattgccgc gtttatggaa aaaaatgccg acatcgcgta aaatatgcgc aaattttgtg
     4141 attcggtcag gtcgtctgaa acagattgcg cctgatttat tttttcggaa aaccctatga
     4201 gcgaacaaaa ccatccgcaa accgagccgc agttggacga aaaccaaatc atcgccctgc
     4261 gccgcgaaaa actgcacaac atccgccaac agcgcaacgc ttaccccaac gatttcaagc
     4321 gcgacagctt tgccgccgat ttgcaggcgc aatacggcga aatcggcaaa gaagaactcg
     4381 acccgcaggc cgttcccgtt aaaattgccg gccgcatgat gctcaaacgt caaatgggca
     4441 aagccagctt cgccaccatt caagacgtta ccggtcaaat ccagctttat ctgaacaaca
     4501 aaggcgtgag ccaagaagtt ttggacgact tcaaccactg ggacttgggc gacatcgtcg
     4561 gtgcggaagg cactttgttc aaaaccaacc acggcgaact aaccgtgcgt gtgtccgaca
     4621 tccgcctgct gtccaaatcc ctgcgtccgc tgcccgacaa acacaaaggc ttgagcgatc
     4681 aggaaaccaa ataccgccaa cgctacgtcg atttgattgc caatgaagaa tcgcgcaata
     4741 cctttatcaa acgcagccaa atcatccaat ctgtgcgtaa ttttatggtg ggcgagcatt
     4801 atctcgaagt cgaaaccccg atgatgcacc cgattcccgg cggcgcgaca gcgaaaccct
     4861 tcgttaccca ccacaatgcc ttggacattc cgctctacct gcgcatcgcg cccgagctgt
     4921 atctgaaacg cttggtggtc ggcggcttgg aacgcgtgtt tgaaatcaac cgcagcttcc
     4981 gtaacgaggg catgtccgtg cgccacaacc ccgaatttac gatgattgaa ttttacgaag
     5041 ccttctccga ctacgaacgt atgatgcaga tggcggaaga catcatccgc aacgcatcgc
     5101 gcacggtaaa cggcacggca aacatcactt acaacggcaa agaagtcgat ttggaaagcc
     5161 cgttcgaacg tctgaccatc ctcgaagcca tcaaaaaata caatccgcac tacaccgacg
     5221 agcagttgaa cgatgcggaa tggctgaaaa aagaaatcgt caaacacggc gaaagcctgc
     5281 cgccgtcccc gggcatcggc agcctgcaac tcgccctgtt tgaaggttgc gccgagggca
     5341 aactgtggaa tccgaccttt attgtcgatt acccggtcga agtttccccg ttggcgcgcg
     5401 cttcggatac gaaacaaggt ctgaccgaac gcttcgagct tttcgttgtc gggcgcgaac
     5461 tggcaaacgg ctattccgag ttgaacgacc ccgaagacca agccgaacgc ttcaaatcgc
     5521 aagtggcgca aaaagatgcg ggcgacgacg aagccatgca ctacgatgcc gactacatcc
     5581 gcgcgatgga attcggtttg ccgccgaccg gcggttgcgg catcggcatc gaccgcttag
     5641 tgatgctgct gaccgatttg caaaccatcc gcgatgtgat tctgtttccg cagatgcgtc
     5701 ccgagtaatc
//
LOCUS       DAWTCI010000103         5229 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWTCI010000103 DAWTCI010000000
VERSION     DAWTCI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.103"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(406..1248)
                     /locus_tag="WM481_001962"
     CDS             complement(406..1248)
                     /locus_tag="WM481_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFA9081201.1"
                     /translation="MLICNPYEVVIHGTTSSGKIFRPGDWAERLCGILSSFTKDNRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNDPANERVLLAQAIEEKHAAEKTQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDIGRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLAGGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKISAETGVTKIHLNVHVNHDRTDAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            1483..2403
                     /gene="pyrB"
                     /locus_tag="WM481_001963"
     CDS             1483..2403
                     /gene="pyrB"
                     /locus_tag="WM481_001963"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFA9081202.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     VQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELEEADCRYRALGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRTDEIHPDVD
                     AAPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            2413..2871
                     /locus_tag="WM481_001964"
     CDS             2413..2871
                     /locus_tag="WM481_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFA9081203.1"
                     /translation="MEAQKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLTLPDEI
                     AEVFRCPNPNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYNRDSVAEA"
     gene            2933..3598
                     /locus_tag="WM481_001965"
                     /pseudo
     CDS             2933..3598
                     /locus_tag="WM481_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4003..5226)
                     /locus_tag="WM481_001966"
     CDS             complement(4003..5226)
                     /locus_tag="WM481_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFA9081204.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPDAPQRYTVKQGDTLW
                     DISGKYLYSPWQWGSLWGANRDQIHNPDLIYPGQVLVLRYVGGEPRLDLEQTDGIPVV
                     KMSPDKEVSGYGIPAVNANFYRIFIRHPQIVSRKETAAAPRLLSGPEGRLLYTKGARV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAEE
                     RLKDNEYYTRTHPLITPVRTRSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMVPHEP
                     SRPVQAKIVSVFEGVGVGGQFKTITIDKGGDDGLDKGAVLSLYKRKKTMQVNLSNNLT
                     EEPKSRDTVELISTPAEEVGLAMVYHTAPKLAYAIILENISDISEGDTAANPGRDLDN
                     MPDQGRARVDSDPFQ"
BASE COUNT         1129 a         1348 c         1578 g         1174 t
ORIGIN      
        1 cgcagacagt acaaatagta cgaaaccgat tcacttggtg cttcagcacc ttagggaatc
       61 gttctctttg agctaaggcg gggcaacgcc gtactggttt ttgttaatcc actataaata
      121 gcgagcatcg attcacggtg cgctttagtg caaagcgtac aacgcgagcc tgaaccacca
      181 gccgcaatag gaaaagaaag ccgatagagt gcaatgcttg ccaacgcgca agcgagcttg
      241 caagaacgct tggctcaacg agagcaggca agacagaaag caggatagga gaggcaacgc
      301 aaaggtctcg ggctttgatt tcgccgtaaa cctgagtgcc gccttgtccg gacggggtgg
      361 ttcaggcggc gagtgccgac agggtgcaga tgaggaggag tattttcatt tggggtcgca
      421 acggaagtgg taggcgcaga tttcaaaacc gtttttgaaa tacaggcggt gcgcgtcggt
      481 acggtcgtgg ttgacgtgga cgttaaggtg gattttggta acccctgttt ccgcgctgat
      541 tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt gcggcagggt
      601 aacgatgtcg tcgatgtgga tgtggcggcc gccggcgagg gtgcaggctt cgcggaagcc
      661 gcagacagcg acggcgttgt gtttgccttc ttcaaaaatg cccagcaggc ggtagccttg
      721 ggggcgttgg gttttgttga tctgttcggt aaagcggccg atgtcggtca gggcggaacg
      781 caaaacactc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt cgcgtaaaac
      841 gtaggatgcg cccgaggcgg tctgttcctg tgttttctcg gcggcgtgtt tttcttcgat
      901 tgcctgtgcc agcaggacgc gttcgttggc agggtcgttt tgtccgccct gttcgcgttc
      961 ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa agtccatcag
     1021 gaagcggaac atctgcggat tgtcggtttc cagtttttta tcgacggcga cgcagcggat
     1081 gttgtccacc agcatggggc gcacccattt cgaataggag aggcggttgt ctttggtgaa
     1141 cgaggacagg atgccgcaca ggcgttccgc ccagtcgccg gggcggaaaa tcttgccgga
     1201 actcgttgtg ccgtggatga cgacttcgta ggggttgcag attaacatgg cggcttcctg
     1261 aaaagaaatg tctggcgcga ttatacctta tgcttatgcg ggcgtgtttg gatatgccgt
     1321 ctgaaaaata caggattcgt gcggtaaaac tttgtggagg caaatgtgcg ataatacgcg
     1381 ccgtgtcgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg acgtataacc
     1441 ggatgcccgc ctgacgcggg ttttttacgg aaggggggca aaatgcctaa tccgctttac
     1501 agacagcaca tcatctccat ttcggatttg tcgcgcgaac agttggaatg cctgcttcag
     1561 acggcattga agctgaaggc gcatccgcgc ggcgatttgt tggaaggcaa actcatcggt
     1621 tcgtgctttt tcgagccgtc cacgcgcacg aggctgtcgt ttgaaacggc ggtgcagcgt
     1681 ttgggcggta aggtcatcgg cttttcggac ggcgcgaata ccagtgccaa aaaaggcgaa
     1741 acgcttgccg ataccgcccg tatcatttcc ggctacaccg acgcgattgt ccaacgccat
     1801 cccaaagacg gcgcggcgcg cgtggcggcg gagttttcgc gcgtccccgt catcaacgcc
     1861 ggcgacggca cgaaccagca ccccagccaa accttgttgg acctggtaac gatttatgaa
     1921 acgcaggggc gtttggacaa gcttaaaatc gccatggcgg gcgacttgaa atacgggcgc
     1981 accgtgcatt cgctgtgtca ggcgttgaaa cgctggggct gcgaatttgc cttcgtctcg
     2041 ccgcccagcc tcgcgatgcc cgactacatt accgaagagt tggaagaagc cgactgccga
     2101 taccgcgccc tcggcagttt ggaagaggcg gcggaatggg cggacatcct gtatatgacc
     2161 cgcgtccagc gcgaacgttt cgacgaacag gaatttgcca aaatccaagg caaattcaac
     2221 ctcgaagcgt ctatgctcgc ccgcgccaaa ccgaacctgc gcgtgctgca ccccctgccg
     2281 cgcacggacg aaatccaccc cgatgtcgat gccgcgccgc acgcctatta tttcgagcag
     2341 gcgaccaacg gcgtttatgc gcgtatggcg atattgtcgc tggtgttgaa cgaagaagtg
     2401 tgaggaaccg atatggaagc gcaaaaactc agtgtcgaag ccatcgaaaa aggcacggtt
     2461 atcgaccata ttcccgccgg cagggggctg accatcctgc gccagttcaa acttttgcac
     2521 tacggcaacg cggtaaccgt gggcttcaac ctgcccagca aaacccaggg cagcaaagac
     2581 atcatcaaaa tcaaaggcgt gtgcttggac gataaagccg ccgaccgcct cgccctgttc
     2641 gcccccgaag cggtggtcaa caccatcgac aatttcaagg tcgtacagaa gcggcatttg
     2701 accctgcccg acgaaatcgc cgaagtgttc cgctgcccga atcccaattg cgccggccac
     2761 ggcgagccgg tcaaaagccg gttttatgtt aagaaacaca acgggcagac gcggctgaaa
     2821 tgccactact gcgaaaaaac ctacaaccgg gattcggtgg cggaagcctg acggattccc
     2881 gagcccggcg gcggaatttt gcctgccgcc tgttttgcca atctgaaatg gaatgatgat
     2941 gcacgcttct gtccaaagtc gtttcgcacc gatactttat gttttgattt tctttgccgg
     3001 ttttttgacc gcgcaaatct ggttcaatca gaaagcctat actgaagagc tgcctccgct
     3061 tctgtccgca ttgtccgccg tcgcgctggt gtggctggcg tgggcgttcg tgtcggtgcg
     3121 ttcaaaggct aaggcagaaa agttctaccg cgaaaaaatg atacagaacg aaagcatacc
     3181 cccgtcctgc acgcttcttt gcaacacttg gaacacaagc cgcaaatgct cgccctgctg
     3241 gtcaaaaacc acggcaaagg catggcggaa caggtcaggt tcaaggcgga agtgctgccc
     3301 gacgacgaag acgcgcgcac gattgccgcc gagttggcaa aaatggatat gttcgcattg
     3361 gggacggacg cggtcgcctc gggcgaaacc tatgggcgcg tgttcgccga tattttcgag
     3421 ttgtcggcgg ctttggaagg gcgcgcgttc aaagggatac tgaaactgac ggcggaatat
     3481 aaaaaacatc ttcggcgatg cctgccgttc ggaaacggcg ttggatttgg gcgcgctcaa
     3541 tcaggcgttg agggaaatct cgaaaacgcc ggaaaagcct aagcagatat tttattgaag
     3601 gtgtcccgat gtatggggag cgcacgggtg gcgcggcggc aaagcggcaa gattgggttt
     3661 ggatacccgg ttggttttat agtagattaa caaaaatcag gacaaggcgg cgggccgcag
     3721 acagtacaaa tagtacggaa ccgattcgcc cggtgcttgg acaccttagg gaatcgttcc
     3781 ctttgagccg gggcggggca acgccgtacc ggtttttgtt aattcgctat atctgtctgc
     3841 aagggagaca gccgctgtga tttccgttcg ggcggaaacc gattccatcg gataaaaggc
     3901 attttgtccg actaaatcag ggttttgcat caacgggata tgaaaacaca gcctaaataa
     3961 aaatgccgtc tgaatgctcc agacggcatt gttgccgagg ggttattgga aagggtcgga
     4021 atcgacgcgg gcgcggccct gatccggcat attgtccaaa tcccgtcccg gattggcggc
     4081 ggtgtcgcct tcggaaatat cggagatgtt ttccaaaata atggcgtaag ccagtttcgg
     4141 cgcagtgtgg taaaccattg ccaagcccac ttcttcggca ggtgtcgaaa tcagctcgac
     4201 ggtatccctg cttttaggtt cttcggtcaa attgttggag aggttgacct gcatcgtttt
     4261 cttgcgtttg taaaggctta aaactgcgcc tttgtccaaa ccgtcgtccc cgcctttgtc
     4321 tatcgtaatg gttttgaact gtccgccgac ccccacgcct tcaaacacgg aaacgatttt
     4381 tgcctgaacc gggcgggacg gttcgtgcgg caccatattg aagcggtcgg tgtcttccgg
     4441 cattttcatc aggtagtcgc cctgctgtat ttcggaaatg gcggtttcga ccaccagcgg
     4501 ctgtatcgaa cgggtgcgca ccggggtaat caaaggatgg gtgcgggtgt agtattcgtt
     4561 gtctttcagc cgttcttcag cctgtttcga gcgttgttcg agggcggagt cggtatagtc
     4621 gagggagcgc acgatgccgc tgaatgcgac ttcttgtccg aggaatttac ccgtatccgg
     4681 atcggtgatg tttttattga tgcggtaggt caggtagcgg cccggctctt tcaggccttt
     4741 ggtgtaaacc ctggcgcctt tggtgtacag cagcctgcct tccgggcccg agagcaggcg
     4801 cggcgcggca gcggtttctt tgcgggaaac gatttgcggg tgccgtataa agatgcggta
     4861 gaagttggca ttgacggcag ggatgccgta tccggacact tctttatccg gactcatttt
     4921 gacgacgggg atgccgtctg tctgttccaa atcaaggcgc ggttcgccgc caacgtagcg
     4981 caacaccaat acctggcccg gataaatcag gtcgggattg tggatttgat cccggttcgc
     5041 gccccacagg ctgccccatt gccacgggct gtacaggtat ttgccggaga tgtcccacag
     5101 ggtgtcgccc tgtttgaccg tgtagcgttg cggcgcatcc gggcgcacct ccagatttgc
     5161 cgccaaagtt tgtgttgaga atgccatgcc tgccgcgcag agcagggtta taatacgacg
     5221 ttgcataac
//
LOCUS       DAWTCI010000104         5167 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWTCI010000104 DAWTCI010000000
VERSION     DAWTCI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.104"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(393..469)
                     /locus_tag="WM481_001967"
     tRNA            complement(393..469)
                     /locus_tag="WM481_001967"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(433..435),aa:Asp,seq:gtc)
     gene            complement(502..577)
                     /locus_tag="WM481_001968"
     tRNA            complement(502..577)
                     /locus_tag="WM481_001968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
     gene            complement(617..693)
                     /locus_tag="WM481_001969"
     tRNA            complement(617..693)
                     /locus_tag="WM481_001969"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Asp,seq:gtc)
     gene            complement(726..801)
                     /locus_tag="WM481_001970"
     tRNA            complement(726..801)
                     /locus_tag="WM481_001970"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(766..768),aa:Val,seq:tac)
     gene            complement(871..2847)
                     /locus_tag="WM481_001971"
     CDS             complement(871..2847)
                     /locus_tag="WM481_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFA9081205.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPELEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVEAMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMHPNMPVYARATN
                     DDYVKTFTDMGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2993..3832
                     /locus_tag="WM481_001972"
     CDS             2993..3832
                     /locus_tag="WM481_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFA9081206.1"
                     /translation="MTATAADLDRLLPQTQCRECGYEGCLPYARAMLRGEAHNLCAPG
                     GATVVRDLAALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVITDECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSDDSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPEKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRQGYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            4031..5062
                     /gene="gap"
                     /locus_tag="WM481_001973"
     CDS             4031..5062
                     /gene="gap"
                     /locus_tag="WM481_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFA9081207.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAVSEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1332 a         1680 c         1196 g          959 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaggtctt ttctaaaata tgcagatatt cgaatgtcga
       61 atttgcctta tgattacggt tttctgtctt atccgcctta aaacgccggt attccgtttg
      121 aaccaaatta tatttgccga aacactcaaa aatctccctt acctgcccga cagacatcaa
      181 gacttcgttg ttgtaactga gaaaaatata ttgaaagtcc gcatccctaa tcaaagcctc
      241 aaataccgga accacatccg attttgatca agtccgcgct aaaacacatc gtacagcgca
      301 aaaccggatg ggaatggcaa aatccggaaa caaacatttt tcaaagcgta caaacaaaaa
      361 ataccaaccg gaggttggta tttcttaact tttggcgcgg cggacggggc tcgaacccgc
      421 gaccaccggc gtgacaggcc ggtactctaa ccaactgagc taccaccgcg catccattgt
      481 ttgcaccaat gaaaggaaac ttggtgggtg atgacggagt cgaaccgccg acattctgct
      541 tgtaaggcag acgctctacc aactgagcta atcacccgtg tataaaaaat accgacagag
      601 tcagtatttt gaaacttggc gcggcggacg gggctcgaac ccgcgaccac cggcgtgaca
      661 ggccggtact ctaaccaact gagctaccac cgcgcatcca ttgtttgcaa caatgaaagg
      721 aaacttggtg ggtgatgacg gagtcgaacc gccgacattc tgcttgtaag gcagacgctc
      781 taccaactga gctaatcacc cttgcgattt gcgaaaacgc tattagacca aataaccggg
      841 aatggcgcaa gccttatttc ggcatttcat ttatattccc tgcaagctcc agttttcaaa
      901 agagataatt tcttcttttt tgcctgcgac caccaacacg tcaccgcctt ccaatgtaaa
      961 cgaggcatcc gggttttcaa tccggccggt gcggcggcgg acgaacaaga gtttgatgcc
     1021 gtaagccgcc atcggaagcg tgccgactgt tttgccgacg gcgtatgctt ctgcagccaa
     1081 aggaaaggcg tggcggacgg tttcgccatt ttcgccgaat cctgcctcat catcactacc
     1141 gacaaacagt ccctccaacg cggcataacg gctgtggcgg atatttgcca tcgtctgata
     1201 gacgtgccgg tacgacgcgc cgttacccaa catcgcatag cctgccagca cgagtccggt
     1261 ttctttggtg tccgacacgg cttcttccgc acccatatcg gtaaacgttt tcacataatc
     1321 gtcattggtg gcgcgcgcat atacgggcat attgggatgc atggacagca cattgtctaa
     1381 aacgtgttgc gtttcgtgca tattgttgag cgtaaccacc accattttcg cccgtcccag
     1441 accggcggct tccaatactt ccctgcgttt cgcatcgccg aacgacaccg gctcgcctgc
     1501 acttctggct acctgcacgc gtgcgatgtc caagtcgagc gcgaaatacg gaatatcctc
     1561 ttgggcgagg acgcgtccga ccgtctgccc gcccctgccg aagccgacaa tcagcacatg
     1621 atcagacttg ctcatcgctt ccaccaacat gctgtgcaga tcgagcgact tcatgtccca
     1681 gcttgacttg accaagcgcc cgaccagcgc atcgctgccg cccaagagga agggcgcgat
     1741 aatcatcgac agcagaaccg ccgccgtcgc cgcctgttcc aattctggcg aaaccatatc
     1801 aagctgcccg gcaatggcca gcatcacgaa gccgaactcg ccgccttgcg cgagatacaa
     1861 agccgttttg aggctgtcgc cgaccgaatg cttcattttg aatgcaatgg caaacacaac
     1921 caatgccttc aacaccagca gcattgccaa cagcatcaat acctgccgcc agccgccgat
     1981 caatgcctga atgtccagct tcatgccgac cgtgataaag aagaagccga gcaaaatatc
     2041 gcggaacggg cgaatgtcgt cttcgacttg gaaacggtat tccgtttccg aaagcaacat
     2101 gccggcaacg aatgcgccca acgccataga caaaccttcc agctcagtca gataagccac
     2161 acccaaggtt accagcagca cattgatcat aaagagttcg gacgatttgc gttttgccac
     2221 catcctgaac catcgcgaca taattttgct gccgacgaaa aacagcagcc ccagcgtcag
     2281 cagcattttt gcaaacgcca aacccaaggc cgcccaaata tttccgtccc ctccgccagc
     2341 cagcgcggga atcagaatca tcagcggcac gacggcgatg tcctgcatca gcagcacgcc
     2401 catcgccatc tgaccgtgcg gctgccccaa ctccgtcttt tccgacaaaa tccggctcac
     2461 aatcgccgtg gacgacatcg ccaacgcgcc cgatacggca aacgcccaat tgaacggcac
     2521 gcccgtcagc atcagtatgc ccattaccga cagcatcgta acgccgacct gcaaaccgcc
     2581 cagaccgaac accagccgcc tcatcgccct cagcttgggc aatgaaaact ccaaaccgat
     2641 gctgaacatc aggaacacaa tcccgatttc gcccaaataa tccgtcgcat ggcttttcgg
     2701 aatcaggctg agcataccgg gccccgccaa aaagcccacc agcaggtagc ccagcatgga
     2761 gggaatgttg aacttgcggc acaggatcac cgtaatgacc gacaccagca gaacaaccac
     2821 aataggggca agcgaaaatt cgttcatgga ccgtccgaac aggaaaatac agaaaaatgc
     2881 cgtctgaaac ggcatacgcc gccgtattat aacaaaacac cgcgcaccat ccgaaacggg
     2941 cgcggcatac aattctgata aaatacgccc ttttgacccg caccaccgca caatgaccgc
     3001 caccgccgcc gatttagacc gactcctccc ccaaacccaa tgccgcgaat gcggctatga
     3061 aggctgcctg ccctacgccc gcgcgatgct gcggggcgag gcgcacaacc tctgcgcccc
     3121 gggcggcgca accgtcgtcc gcgaccttgc cgccctgctc ggcaaacccc ttgtcgcacc
     3181 tgccaaaacc caagccaaag cactcgcccg gatagacgaa accgcctgta tcggctgcac
     3241 cgcctgcatc cgcgcctgcc ctgccgatgc cattatgggc gcgggcaaac ttatgcacac
     3301 cgtcatcacc gacgaatgca ccggctgcgg actctgcgtc gccccctgcc ccgtggactg
     3361 catccatatg cagcccgttg ccgacaccgt cctgccgcgc gcgcgccgtt tcagcctgtc
     3421 cgacgacagc cgttttgccg ccgccgaaca cgcgcgtgcg cgctacctca aacgcaacga
     3481 acgcaaacag cgcgaagccg acgaacgcaa ggccatgctt gccgaacgcg aagccgccgt
     3541 ccgcaacgcg cgtccgcaaa cgcccgacac accggaaaaa ccggcgttca accccgccga
     3601 cctcatcgcc aaagccatgg caaaagcgca aacccaacag gatcgcctcg ccgccgccga
     3661 caaccgccaa ggctatcagg caaaacagat agccgaagcc cgcgaacgcg ccgaattgcg
     3721 ccgcgcccaa cgcgatatga aatacggcag cgacagcgaa aaagccgccg ccctcgaata
     3781 cctcaaacaa tacaaagcca aacaggaagc cgcacagaat accgcctcct gacccttacc
     3841 cgacatgccg tctgaagccg cttcagacgg cattttatca agctctccgt ccgccgtacc
     3901 cgtgccgtcc gtatcttacc gcccaccctt ccggccgtgc cgttttcaat aaaatattaa
     3961 ttacacgcca ctacaaattt gctataatcc gccccgaaaa tctacccaac cctcaacaaa
     4021 ggaacaaacc atgggcatca aagtcgccat caacggctac ggacgcatcg gccgccaggt
     4081 tttgcgcgcc atctacgact atcagattca agaccaactc caaatcgtcg ccgtcaacgc
     4141 cagcggcagc cttgaaacca acgcccatct gaccaaattc gacaccgtac acggacgctt
     4201 tgaagccgac gtatcccacg acggcggcaa cctcatcgtc aacggcgaca aaatcccctt
     4261 cttctcgacc cgcaaccctg ccgaactgcc gtggaaagaa ctcggtgtcg atttggtcat
     4321 ggaatgcacc ggcgcgttca ccagcaaaga aaaagccaaa atccacctcg aaagcggcgc
     4381 gaaaaaagtc ctcatttccg caccgggcgg cgacgatgtc gatgcaaccg tcgtgtacgg
     4441 cgtgaacgac agcgtcctga ccgccgacat gaccgtcgtt tccaacgctt cctgcaccac
     4501 caactgcctc tcgccggttg ccaaagtgtt gagcgaaagc gtcggcatcg tcaaaggcgc
     4561 aatgaccacc atccacgcac tgaccaacga ccaaaccgtt accgacgtgc gccacaaaga
     4621 cctgcgccgc gcccgcagcg gcgtggaaaa catgattccg accaaaaccg gcgcggcaaa
     4681 agccgtcggt ttggtcttgc ccgaattgaa aggcagactc gacgggcttg ccatccgcgt
     4741 gccgaccgtc aacgtatcct tggtagattt gagcttccaa gccgcgcgcg acaccacagt
     4801 cgaagaaatc aacgcactga tgaaagccgt ctcggaagca ggcgcgctca aaggcgtttt
     4861 gggctacaac accctgccat tggtttccat ggacttcaac cacaccaccg aagccagcca
     4921 cttcgacgca acgctgacca aagtcgtcga cggcaacatg gtcaaagtgt tcgcgtggta
     4981 tgacaacgaa tggggcttca gctgccaaat gctgaacacc gcacgccgta tgttcggact
     5041 tgaagtgcgc ccgctcaaat aagcaacaaa ccgtcaaaca aaatgccgtc tgaaacccga
     5101 tgttttcaag tttcagacgg cacttttcat ttttgaccgc gattttaccc ggctgtcgtc
     5161 atttcca
//
LOCUS       DAWTCI010000105         4840 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWTCI010000105 DAWTCI010000000
VERSION     DAWTCI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.105"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..154)
                     /locus_tag="WM481_001974"
     CDS             complement(<1..154)
                     /locus_tag="WM481_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081208.1"
                     /translation="MKKSLFAAALLSLALAACGGEKAAEAPAAEASSTEAPAAEAPAA
                     EAPAAEA"
     gene            627..1109
                     /locus_tag="WM481_001975"
     CDS             627..1109
                     /locus_tag="WM481_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFA9081209.1"
                     /translation="MAIAKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSSDDAFGEQDSELVRIEDAGAFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGSHGHYHH
                     "
     gene            1196..2008
                     /gene="hpnC"
                     /locus_tag="WM481_001976"
     CDS             1196..2008
                     /gene="hpnC"
                     /locus_tag="WM481_001976"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFA9081210.1"
                     /translation="MSVGHYENFPVGSLVLPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLSGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGDLTDYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEEQIAAGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(2081..2551)
                     /locus_tag="WM481_001977"
     CDS             complement(2081..2551)
                     /locus_tag="WM481_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFA9081211.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLGALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     PEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            2642..4483
                     /locus_tag="WM481_001978"
     CDS             2642..4483
                     /locus_tag="WM481_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFA9081212.1"
                     /translation="MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELTVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPAFMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKFSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         1063 a         1081 c         1440 g         1256 t
ORIGIN      
        1 gtgcttcggc ggcaggtgct tcggcggcag gtgcttcggc agcaggtgcc tcggtagagg
       61 aagcctcagc agcaggagct tcagcggctt tttctccgcc gcaggcagcc aaagccaaag
      121 acaacaaagc agcggcaaac agggattttt tcattttagt aacctttaaa atcaatttaa
      181 attattgaaa caaataggtc tttttcgacc atactacaaa ccgggttgga aattcatcgc
      241 tgaatttgcg taattatgca ccatggaata agggattgca atgtcgtaca gatgggaaaa
      301 aggtttgaat gaaaagtttt ctggcgggcg gattgataaa tcctgtaaaa cggttttgct
      361 gttaaaaatc cgatagggat aaatattata gagattaata taattcagta tcttgtatga
      421 tttattaagg cagattgggg aagtcggcag atgggtcgga atggtttttg agagtgttgt
      481 aaaaaattta cactgcaata aaataacaaa aatttacatt gaaatatgtg ccgtacagtt
      541 tatgaacagg ttaacggtaa tcggccggcg cggcggttgt gttagaatgc gcctgtaatc
      601 aatctgctgt tcctgaaagg acaaatatgg ctattgcaaa aaattccgtg gtttcgctgc
      661 attatgagat gtatgatgcc aacaatcagc ttttggataa aaccgaagaa ccgattgcgt
      721 atctgcacgg cggttacgac ggcattttcc ctttggtgga agaggcgttg cacggtaagg
      781 atgccggcga tacggtcgat gtggcgcttt cgtccgacga tgcgttcggc gagcaggatt
      841 cggagttggt ccgtattgaa gatgcgggcg cgttccctgt tgaagtcgaa gtcggcatga
      901 tgtttgaagc cgacgatcct gaaaccggcg atgttgtcgt ctatcgtgta accgatgttg
      961 ccgacggcaa ggcggtggtg gacggcaacc atcctttggc aggcatgaaa atccgcttta
     1021 aagctacggt tgaaagcgtg cgcgatgcat ccgatgagga aatcgcacac ggtcatgtcc
     1081 acggttcgca cggtcattac caccactgat tgccggcagc ttggaggtat ctgatgccgt
     1141 ctgaaaaggg tttgttccgt ttcagacggc attttgtttg atggatggag aaggtatgtc
     1201 ggttgggcat tatgagaatt ttcccgtcgg ttcgctggtt ttgccgcgca ggttgaggaa
     1261 gccggttcat gcggtgtatg cgtttgcacg gacggcggac gatatggcgg acgagggcag
     1321 tatgccgtct gaagccaggt tgtcggggtt ggagggtttg cggcgcgagt tggacgtgtt
     1381 ggcatcgggc ggccggtcgg cgcatccttt gattgcacga ttggatgccg aggcggttgt
     1441 gccgttcggt ttggatttgc agccgtttta tgatctgctc tcggcgtttt cgcaggatgt
     1501 ggttaaaacg cggtacgcgc atttcggcga tctgaccgat tattgccggc gttccgccaa
     1561 ccctgtcgga cgcattatgc tggctttata cgggaaaacg gatgcggtgt gcgtagcgca
     1621 aagcgacggt atttgtacgg ctttgcaact ggtgaatttt tggcaggatg tagctgtgga
     1681 ttggcaaaag ggcagggttt atatcccgca ggacgatttg ttgaaattcg gtgtttctga
     1741 ggaacagatc gcagcaggac gggcggatgc ggcgtttcag cggctgatgg cgtatgagtg
     1801 ccggcgtgca ttccgtatgc tgaaggcggg ttcgcctttg gcgcgcgaat tgaaagggcg
     1861 tatcggtttg gaactccgta tgattgtgtt gggggcgcag ttgattttgc agaaactgga
     1921 cgcgtgccga tacgatgtgt ttgcacaacg ccccgttttg gataagaagg attggttgat
     1981 tatgttgaaa cgcgcgttgt ggaaatgatt gggggaaaat gaatgccgtc tgaaaggggt
     2041 tgttccgctt cagacggcat tttgcaggcg gtcggtatgg tcaggcgatt tcatgcgggt
     2101 ttaacctggc ttgatcctct gcacccaagc cgatgagttt gcgcagtcgg gtcatgtaga
     2161 aattgacatc caaaccggtg ccgtagcgtt gcgccgtcca cagtgtttcc gccagtgcct
     2221 ccatcatttc gtgttcggct tccggccagc cgcgtttggc gcacagggtg tcgtggatgg
     2281 cgcgtatgcc gtgcggctgg tctatgcccg cctgttcttg gacggacaaa tgcagcgaca
     2341 tatgcaggaa ggggttgctt tcgccgtttt cgggcagcca gtcggtgtcc agatggtctt
     2401 cgatgcgttc gagataacgg tggtattcgg gatgggcttc gacaatgcgg agggctttct
     2461 gttccaacgc gcccaattgc agcggattga gccgctgctg ccacacgcgg gcgaaaaagc
     2521 ggcggacatc gtgggtattg acatcgtaca tggcgggtcc gataagatgt ttatggaaat
     2581 aggttcggat aatggcgggt tttacataaa ttaaatccta ccgtcaacct taccatttat
     2641 aatgcggaca aattatttgt ctgaaacgga ttcgattatg aaaaaactga tttgcctgtt
     2701 cgccgtattt ttgatgttgt gcggacgggc tttcgcgctg gatgcgaacg atctgctgcc
     2761 gccggaaaag gcattcgtgc cggagcttac tgttgccgac gacggcgtga acgtccgttt
     2821 caggattgcc gacggatact atatgtatca ggcgaaaatc gtcggcaaga ccgatccggc
     2881 ggatttgttg ggacagcctt ctttcagtaa gggcgaagag aaggaagacg agtttttcgg
     2941 caggcagacg gttcaccatc acgaggcgca ggttgccttt ccttatgcaa aggctgtcgg
     3001 cgaaccgtat aaattggttt tgacctatca gggctgtgcc gaagccggcg tgtgctatcc
     3061 gcccgtggat accgagtttg atatttccgg caacggcact taccatccgc aaaccgacga
     3121 accggcatcc gccaaagacc gttttttgca gccttcctct caaaacggca gcggggcgtt
     3181 gccgcccccg aagggggacg agggcggcga cggccgtttc aagctgtctt gggatacgct
     3241 caacgccaat cttttggcgt tttttctcgc cggtttgggc ctgagtttta ccgcttgtat
     3301 gtatcccctg ctgccgattg tgtccagtat cgtggtcggt gacaaaaagg cgggcaaggc
     3361 gcgggcgttt gtgctgtccg tcgtttatgt tcagggtttg gctctgactt atacgctggt
     3421 cggcattgtt gccggactga cgggcgcgct gctgaccgta tggttgcagc aggcttgggt
     3481 cgtgttggcg gcatcggctt tgatggtcgt cttggcgctg tctatgttcg ggctgttcaa
     3541 catccagctt cccaacgccg tacagtcata tttccaaaac cagagcagca ggctctccgg
     3601 cggtaaaatc gtttccgtct ttattatggg cattctgtcc gcactgattg tcggaccgtg
     3661 cgtcgccccg ccgctggcgt ttgccttggg ctatatcggt cagacgggcg atgcggtttt
     3721 gggcggtttg gcactttaca ctttggcgtt gggcaccggc gtgccgctga ttgccatcgg
     3781 cacgttcgga ggacatatcc tgcctaaggc gggcgattgg atgaatgccg tcaaatacgc
     3841 tttcggcttt atcctgcttg ccgtcgccgt ttacctcgcc acgccgcatt tgccctatta
     3901 tctcgtcgtc gcgctgtaca cgctgctgat gctggttcct gcctttatgc tgctggtcaa
     3961 cggacgcagg cagaaacgcc gtccgaaagc tgtggcattc gcattgggca gcatattgct
     4021 gataggcggc gcgtggttcg gctggcaggg cgcaaacggc aaaacgaccg cgctgcacca
     4081 tttcttgacc ctcaatccgc cggccgaagc aggcaaattt tcggaacacg gcaaaatgtt
     4141 tgccgatact gccgcgctga aggcagcgat ggatacggcg ttgaaagaac atcccgacaa
     4201 acctgtcgtt ttggattttt atgccgactg gtgcatttcc tgcaaagaaa tggcggttta
     4261 cacgctcaat cagccggaag tgcatcaggc agtcgatatg gaacgctttt tccagatcga
     4321 cgtaaccgcc aacacgcccg aacatcaggc gttgttgaaa gaatacggtc tgttcgggcc
     4381 gccgggcgtg tttgtcgtcc gcgccgacgg cagccgcagc gagccgctgc tgggttttgt
     4441 caaagcagac aagtttatcg agtggtatga acaaaaccgc tgattccgat gtttcaaatg
     4501 ccgtctgaag ttctcagacg gcatttttgt cagacaggaa aacggcaata tccgctatac
     4561 acacggaagc agtccaaaga tgtgaaaacc gtgtgtataa gtcggtttgc gtattgaaat
     4621 taaaagataa aaatacgatg attaaaaaat aggcataaga aaagataaac acagccggtg
     4681 tttgccggtg tgtgtatctt ttgtggaaaa acatcttaat gccatgtatt ttatggaaaa
     4741 taataacatg attaaaaaat agtcaatcat taaggttcag gtaggcggct gcaaccgtca
     4801 atgtctgaaa agaggtagtg ctgtttaagc atggaaagcc
//
LOCUS       DAWTCI010000106         4772 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWTCI010000106 DAWTCI010000000
VERSION     DAWTCI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.106"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            117..986
                     /locus_tag="WM481_001979"
     CDS             117..986
                     /locus_tag="WM481_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9081213.1"
                     /translation="MLRLTTLTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGKGLVVLVNGGKMSAFGPSSRLGG
                     WLHKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLN
                     NPLVAETTAWKKG"
     gene            1106..1297
                     /locus_tag="WM481_001980"
     CDS             1106..1297
                     /locus_tag="WM481_001980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081214.1"
                     /translation="MPSETRMQTASCVSDGIARYGRYRGNGGKRFDWNLPHPLPVRRF
                     QTRLSAPTVRTALFESPTP"
     gene            1316..2284
                     /locus_tag="WM481_001981"
     CDS             1316..2284
                     /locus_tag="WM481_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFA9081215.1"
                     /translation="MTAKPFSLNLANLLLPAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     KNEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIVSRLTGDRLRQSLPAVALLG
                     ASMVLLCDILGRMIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2274..3248
                     /locus_tag="WM481_001982"
     CDS             2274..3248
                     /locus_tag="WM481_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFA9081216.1"
                     /translation="MPSEKNIGFMAGSSRPLRVAFALLLVSCILFMTVNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVRSELLGIGALVLLVSAAVVWRERYRSDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3293..3919
                     /locus_tag="WM481_001983"
     CDS             3293..3919
                     /locus_tag="WM481_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081217.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDSGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVGGSASAKELERFGQLWQARVKAFLTESADDPQFVIVREL"
     gene            4003..4761
                     /locus_tag="WM481_001984"
     CDS             4003..4761
                     /locus_tag="WM481_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFA9081218.1"
                     /translation="MITIRNVSYRIGTHPILDNVSLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLAGTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTHEHKRTTVAVLHDINQAAAYADHVVAMKNGKAALTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
BASE COUNT          978 a         1437 c         1300 g         1057 t
ORIGIN      
        1 cgccttgtcc tgatttaaat ttaatccact atagattatc atttatcctt tctaaagccg
       61 ttccggtttg tccgaccggc ggctttgccc caatatcccc attttggaga caccctatgt
      121 tgcgtttgac cacattgacc ctctgcaccg tcctcgcctt gggcgcgtgt tcgccgcaaa
      181 attccgactc tgccccacaa gccaaagaac aggcggtttc cgccgcacaa tccgaaagcg
      241 cgtccgttac cgtcaaaacc gcgcgcggcg acgttcaaat accgcaaaac cccgaacgca
      301 tcgccgttta cgatttgggt atgctcgaca ccttgagcaa actgggcgtg aaaaccggtt
      361 tgtccgtcga taaaaaccgc ctgccgtatt tagaggaata tttcaaaacg accaaacctg
      421 ccggaacttt gttcgagccg gattacgaaa cgctcaatgc ctacaagccg cagctcatca
      481 tcatcggcag ccgcgccgcc aaggcgtttg acaaattgaa cgaaatcgcg ccgaccatcg
      541 agatgaccgc cgacaccgcc aacctcaaag aaagtgccaa agagcgcatc gacgcgctgg
      601 cgcaaatctt cggcaaacag gcggaagccg acaagctgaa ggcggaaatc gacgcgtctt
      661 ttgaagcggc gaaaaccgcc gcgcaaggca aaggcaaagg caaaggtttg gtggttttgg
      721 tcaacggcgg caagatgtcg gctttcggcc cgtcttcacg actgggcggc tggctgcaca
      781 aagacatcgg cgttcccgct gtcgatgaat caattaaaga aggcagccac ggccagccta
      841 tcagcttcga atacttgaaa gagaaaaatc ccgactggct gtttgtcctc gaccgcagcg
      901 cggccatcgg cgaagagggt caggcggcga aagacgtgtt gaacaatccg ctggttgccg
      961 aaacgaccgc atggaaaaaa ggataggtcg tttacctcgt tcccgaaact tatttggcag
     1021 ccggcggcgc gcaagagctg ctgaacgcga gcaaacaggt tgcggatgcg ttcaacgccg
     1081 cgaaataatg aaacggcggc attcgatgcc gtctgaaaca cggatgcaaa ccgcttcctg
     1141 tgtttcagac ggcattgccc gatacgggcg ttatcgcggg aatggcggca agcgttttga
     1201 ttggaacttg ccccatcctc tgcccgtccg gaggtttcaa acaaggcttt ccgctccgac
     1261 ggttcggact gccttgtttg aatctcctac gccgtaacgc ttttcccttc tgtttatgac
     1321 tgccaaacct ttttccctca acctggccaa cctcctgctg ccggcggtat tgtttgccgt
     1381 cagcctgtcg gtcggcgttg ccgatttccg ctggtcggat gtgttttcgc tgtccgacag
     1441 ccaacaggtc atgttcatca gccgcctgcc gcgcacgttt gcgattgtgt tgacgggcgc
     1501 gtcgatggcg gtggcgggga tgattatgca gattctgatg cgcaaccgtt ttgtcgagcc
     1561 ttctatggcg ggtgcgggcc aaagtgcggc tttgggtttg cttctgatgt ccctgctgct
     1621 gcctgccgcg ccgctgccgg tcaaaatgtc ggttgccgcc gttgccgcgc tgatcgggat
     1681 gttggtcttt atgctgctga tccgccgcct gccgccgacg gcgcaactga tggtgccgct
     1741 ggtggggatt attttcggcg gcgtggttga ggcggtggcg acgtttatcg cgtataaaaa
     1801 cgaaatgctg caaatgctcg gcgtgtggca gcagggcgat ttttcgagcg tgctgctggg
     1861 gcggtacgag ctgctttgga ttacgggcgg tttggcggtg tttgcctacc tgattgccga
     1921 ccggctgacg attttggggc tgggcgaaac ggtgagcgtg aatttgggtt tgaaccggac
     1981 ggcggtgttg tggtcgggtt tgattattgt ggcactgatt acatcgctgg tcattgtaac
     2041 ggtcggcaat attccgttta tcgggctggt cgtgccgaat atcgtcagcc gcctgacggg
     2101 cgacaggctg cgccaaagcc tgcctgcggt cgccctcttg ggcgcgtcta tggtgctgct
     2161 gtgcgatatt ttgggacgca tgattgtgtt tccgtttgaa attccggtct ctacggtttt
     2221 tggtgtgttg ggtacggctt tgtttttgtg gcttttatta aggaaacctg cccatgccgt
     2281 ctgaaaaaaa tatcggtttt atggcaggaa gcagccgtcc gttgcgggtc gcctttgcgc
     2341 tgttgctggt ttcctgcatc ctgtttatga cggtcaacgt caaaggcgat tgggactttg
     2401 tcttgcacct gcgcctgacc aagcttgccg cgctgctgat ggtcgcctat gcggtcggcg
     2461 tgtccactca actcttccaa acgctgacca acaacccgat tctgacccct tcgattttgg
     2521 gtttcgattc gctgtatgtg tttttgcaga ccttgctggt gtttacgttc ggcggcgtgg
     2581 gctatacatc cctgccgttg acgggcaaat tcggctttga actggttgtt atgatgggcg
     2641 gctcgctgct gctgttttac acgctcatcc gtcagggcgg gcgcgatttg ccgcgcatga
     2701 ttttaatcgg cgtgattttc gggattttgt tccgcagtct gtcgtcgctg ctttcgcgca
     2761 tgatagaccc cgaagaattt accgccgcgc aggcgaatat gtttgccgga ttcaataccg
     2821 tccgcagcga gcttttgggc ataggcgcgc tggtcctgct cgtcagcgcg gcggtcgttt
     2881 ggcgcgaacg ctaccgctcg gacgtacacc ttttggggcg cgaccaagcc gtcaatttgg
     2941 gcatcagcta cacgcgcaac accttatgga tactgctttg gattgccgca ttggtggcga
     3001 cggcgaccgc cgtggtcggc ccggtaagct ttttcgggct tctcgccgcc tcgcttgcca
     3061 accacttttc cccgtccgtg cgccattccg tccgcctgcc gatgacggtt tgcgtcggcg
     3121 gcatcctctt ggtcggcgga caaaccgtat tcgaacactt cttgggcatg aaggcggtat
     3181 taagcgtggt ggtcgaattt gcgggcggac tcgttttcct ctatctcgtt ttaaaacaca
     3241 aaaaatgacg gatgccgtct gaacggcagc ccgccccgaa aggacaaacc atatgacgca
     3301 agaacgttta cccgaatttt tcgaccgcgc cccgacgctg accgtacaag acccgcttgc
     3361 cgcattcctc ggcgcggccg aaaacggcat cctcacttac cgctacgccg atgccgtgcg
     3421 cctgtgcgga cattcctgcc cgaccgtcgc gggcgcgtac ctgatggtta tcaaaggcct
     3481 gaaagcactt tacggcgaag agctgcccga acgcggcggc atcgaagccg ccatgcaggg
     3541 aacgcgcgac gaaggcacgg tcggcgtaac cgcgtccgtc gtccaactcc ttaccggcgc
     3601 agcccccgaa accggcttcg gaggcatcgg aatgcaggga cgcttcgccc gccgcaacct
     3661 cttatccttt ggtgcaggcg aaatcaacgg cacactgacc ctgcgccgca aagacagcgg
     3721 caaaaccgtc gccgtcagcc tcaacgccgc cctgcaaccc tttgcacctc aaatgcgcga
     3781 catcatgccc aaagccgtcg gcggcagcgc aagcgcaaaa gaactcgaac gcttcggaca
     3841 actttggcag gcacgcgtta aagcattttt gaccgaatcg gcggacgacc cgcagttcgt
     3901 catcgtccgc gaactgtgaa cgttcagacg gcattccgaa tttcaaatgc cgtccgaacc
     3961 cccgccaaac aacaacaaac ctacgcccga caagcatccg ccatgattac catccgcaac
     4021 gtcagctacc gcatcggcac acaccccatc ctcgacaacg tcagcctcga catccccgaa
     4081 ggcggcatta ccgcgctaat cggccccaac ggtgcgggca aatccaccct gttttccttt
     4141 atggcgcggc tgcgcccgct tgaaagcggc agcatcgcct accgaggcaa aaaccttgcc
     4201 ggcaccccca ccgccgaact cgcccgcacg ctctccatcc tcacccaaga aaacagcatc
     4261 atgagccgca tcaccgtgcg cgacctgctg atgttcggcc gttaccccta ccatcaaggc
     4321 agaccgactg ccgaatgccg ccgtatcgtt aacggtgcaa tcgaagaatt ccacctgcag
     4381 gacctctccg accgctacct gaccgaactc tccggcggac aacgccaacg cgccatgatt
     4441 gcgatggtgt tctgccaaag caccgactac gtccttttgg acgaaccgct gaacaacctc
     4501 gatatgtatc acgcccgctc gctcatgcag attctgcaaa agctcacgca cgaacacaaa
     4561 cgcaccaccg tcgccgtatt gcacgacatc aaccaggccg ccgcctacgc cgaccacgtc
     4621 gtcgccatga aaaacggcaa agccgccctg accggcacac ccgaagaagt gttcaccgta
     4681 cacaacatca aagaactgtt cgacatggac gtggacgtac tcgactacga aggcaaaaaa
     4741 ctcatcgtcc accacatctg aagctcaaaa ag
//
LOCUS       DAWTCI010000107         4666 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWTCI010000107 DAWTCI010000000
VERSION     DAWTCI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.107"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            252..1286
                     /gene="pyrC"
                     /locus_tag="WM481_001985"
     CDS             252..1286
                     /gene="pyrC"
                     /locus_tag="WM481_001985"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFA9081219.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASENGARFYGIPENADTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WM481_001986"
     CDS             complement(1365..1790)
                     /gene="nusB"
                     /locus_tag="WM481_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFA9081220.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(1868..2344)
                     /locus_tag="WM481_001987"
     CDS             complement(1868..2344)
                     /locus_tag="WM481_001987"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFA9081221.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSQMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVANESGAGIGR
                     VALDYNIPIANAVLTTENDAQAIERIGEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(2391..2714)
                     /locus_tag="WM481_001988"
     CDS             complement(2391..2714)
                     /locus_tag="WM481_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFA9081222.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2882..3601
                     /gene="rnc"
                     /locus_tag="WM481_001989"
     CDS             2882..3601
                     /gene="rnc"
                     /locus_tag="WM481_001989"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFA9081223.1"
                     /translation="MKDDVLKRQAHTAIQKKLGYAFRDMSLLRRALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            3648..4571
                     /gene="era"
                     /locus_tag="WM481_001990"
     CDS             3648..4571
                     /gene="era"
                     /locus_tag="WM481_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFA9081224.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRLTDADRVVLKQLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEGDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDNKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT          999 a         1169 c         1438 g         1060 t
ORIGIN      
        1 aattgagggc ggaacagtcg gtgcaggcac cgtttgaaac caggcaaatc agctatgcct
       61 ggcgggaatc gggcagccct gaagaaaccg tattgaccgc acaaaccgcc gattccccgg
      121 atttacccgt acaggcagta ttgaacctaa cgcaccgttc ccataacggg aaacccctaa
      181 ttctcccctg acttcagacg gcataaagtc ggcatgccgt ctggaaacca aaaatctaaa
      241 aaggaacaac catgcaaacc ctgaccatca tccgacccga cgatatgcac ctgcacctgc
      301 gcgacggcga cgcgctcaaa gccgttgccc cttataccgc ccgccagatg gggcgcgccg
      361 tcatcatgcc caacctcaaa ccgccagtcg tcagcgtagc cgacgcgctt gcctacaaag
      421 cgcgcattat ggcggcgttg cccgaaggca gcgcgtttga gccgctgatg acgctttacc
      481 tgaccgacca agccacgccc gaactcgtgc gcgaagccaa agccgccggc atcgtcgcct
      541 tcaaactcta ccccgcaggc gcgaccacca attccgattc cggcgtaacc gacctgttca
      601 agctcatccc cgtgttggaa gaaatggcaa aacagggcat cctgttcctc gttcacggcg
      661 aagtaaccga ccccgaaatc gacattttcg accgcgaagc cgcctttatc gggcgcgtga
      721 tgaaacccgt tttggcgcaa gtgccgaatc tcaaagtcgt gttcgaacac atcaccaccg
      781 ccgaagccgc ccgcctcgtt ttggaagcgg gcgacaacgt tgccgcctcc gtgaccccgc
      841 aacacctcct gctcaaccgc aacgacctct tggtcggcgg tgtgcgcccc catcatttct
      901 gcctgcccgt cctcaagcgc gaaacccacc gtcaggcatt ggtcgccgcc gttaccggcg
      961 agaaggcgca caaattcttc ctcggcaccg actccgcgcc gcacgccaaa tccgccaaag
     1021 aaaacgcctg cggctgcgcc ggtatgttca gcgcgatgac cgctatcgag ctttacgccg
     1081 aagtgtttga aaaagcaggc gcgttggaca aactcgaagc cttcgcctcg gaaaacggcg
     1141 caaggttcta cggcattccg gaaaacgccg acacgattac ccttgtcaaa caaagccaaa
     1201 ccgttcccgc aagcgtccct tacggcgacg gcgaacttgt cccgatgcgc gcgggcggcg
     1261 aaatcggctg gacggtgcag tattgattga acggtcaagc aaaatgccgt ctgaaaggtt
     1321 ttcagacggc atttgtgtat tttccgattc ggattttccg agtatcaacg gcgtttgggt
     1381 tcgtctgggc ggatttgggc ggcgagtttg tcgaggatgc cgttgacgaa tttgtgcccg
     1441 tccgtgccgc cgaaggtttt ggtaacttcg atggcttcgt tgataatgac ggggtagggc
     1501 gtttcgggca tagcggaaag ctcgtggcag gcggtcagca aaacggcgcg ttcgatgggg
     1561 ttgaggtctt tttcgtccct gtcgagcagc gggcggattt tttggatgta gtccgctgcg
     1621 ttggtttgtg tgccgaagaa gagtttgttg aacaattctt cgtccgcttt ggcaaagtcg
     1681 gacatttcgc ggatgttttt agcaatttcg ggcgcggcgg tgcggttgat aagggattgg
     1741 taaacggctt gtacggcaag ctcgcgggaa cggcggcggg ctgttttcat gatttttcct
     1801 tgaaacggtt gggcggcacg gtatgccgtc tgaaacggaa agggcgcatc ggtgtacgcc
     1861 ctgtttgtta ttcttcgtct tcaaactgtt cttcgagcag aaggttgacg aggttggcgc
     1921 attctacggc gactttggcg gcatccgagg ctttttctcc aatccgttca attgcctgcg
     1981 cgtcgttttc ggtggtcagg acggcgttgg caatcgggat gttgtagtcg agtgcgacgc
     2041 ggccgatccc tgcgccggat tcgttggcaa ccagctcgaa atggtaggtt tcgccacgga
     2101 tgacgacgcc gatggcaatc agtgcgtcaa atttttcgga agaggcaaag ttcatcagcg
     2161 cgatggggat ttcaagcgcg ccgggtacgg tggcgacggt gatgttttcg tctgctacgc
     2221 ccaattcttg gagggtgcgg cagcagactt tgagcatttg gctgccgatt tcgttggtga
     2281 agcgtgcctg tacgatgccg atgcggaggt gtttgccgtc gaggttgggg gcgatggtgt
     2341 tcattgggtg tcctttggta ttcggggttt cggaatgccg tctgaaggtt tcagtcttgc
     2401 ggctgccagt cggcaacggt ttggaatgtg ccgtcttcgg caagctccca cgcgctgcct
     2461 tcgggttggg aaagcagtgc ggcggtttca gggttggttt tggtgatgtc ggcgaggctg
     2521 acgatgctga agttgtcggg gtcgtcggtg tattcgtcgg tttcgtcgcc gctgaagaaa
     2581 cgccagccgc tgtcgttttc aaaaacgggg gcttcgcggt aaaggaagcc gacgggccgg
     2641 ttttgtttgg cgacggtgtt ggtggcgatg cagcggtcga gtgccgagga aagtgcttgt
     2701 gcaaatgcgt tcattgcggg aatacgttgg gggggaaact tgcggatttt accacgattc
     2761 ccgcgttgtc ggcagacggc ggcggtttgg tggtacaatg tgcgccgttt gcagccttaa
     2821 ggtgtttctg tatttttgga gtatggaaac gcattcgggc tgttttttgc ggaagacggt
     2881 aatgaaagac gatgttttga aacggcaggc acatactgcg atacagaaaa agttaggcta
     2941 cgcgttccgc gatatgtcgc ttttgcggcg ggctttgacc cacaggagcc atcatgcgaa
     3001 gcacaacgaa cggttcgagt ttgtcggcga ttcgattttg aattatacgg tggcgcggat
     3061 gctgtttgac gcgtttccga agttgaccga gggcgagttg tcgcggttga gggcgagtct
     3121 ggtcaatgag ggcgtgctgg cggaaatggc ggcggaaatg aatgtcggcg acggtctgta
     3181 tttgggggcg ggcgagttga agagcggcgg cttcagacgg ccttcgatac tggcggacgc
     3241 gatggaggcg atgtttgccg ccgtcagctt cgatgccgat ttcaacacgg cggaaaaggt
     3301 ggtgcgccat ttgtttgcag aacgcgtccg gcgcgccgat ttccaaaatc aggcaaaaga
     3361 cggcaaaact gctttgcagg aggcgttgca ggcgcgccgt ttcgccttgc cgaaataccg
     3421 catcgaagag caaatcggcc atgccaacga cagtatgttt gtcatttcct gcgatttggg
     3481 cgaactgggt ttcgtgtgcc gtgccaaagg gacgagccgc aaggcggcgg aacaggaggc
     3541 ggcgaaagag gctttgaaat ggctggaaga gaagctgccg ctgaagaaga aaaagaaatg
     3601 aggcggcgcg tgaatatgcc gtctgaaatg tggatatgaa agcgaatatg gatattgaaa
     3661 ccttccttgc aggggaacgc gccgccggcg gataccgttg cggcttcgtg gcgattgtcg
     3721 gtcgtccgaa cgtgggcaaa tcaacgctga tgaaccatct catcggtcag aaaatcagta
     3781 ttaccagcaa aaaggcgcag acgacgcgca accgcgtaac ggggatttat accgacgata
     3841 ccgcgcagtt cgtgtttgtc gatacgccgg gctttcaaac cgaccaccgc aacgcgctca
     3901 acgacaggct gaatcaaaat gttaccgagg cgctcggcgg tgtggatgtg gtggttttcg
     3961 tcgtggaggc gatgcgcctt accgatgccg accgcgtcgt gttgaaacaa ctgcccaagc
     4021 acacgccggt cattttagtg atcaacaaaa tcgacaagga caaggcgaaa gaccgttacg
     4081 cgctggaggc gtttgttgcc caagtgcgcg ccgaatttga atttgcggcg gcggaggcgg
     4141 tcagtgcgaa acacggtttg cggattgcca acctgttgga gctgctcaag ccgtatctgc
     4201 ccgaaagcgt accgatgtat cccgaagaca tggttacgga caaatcggcg cgttttttgg
     4261 cgatggaaat cgtgcgtgaa aaactcttcc gctatttggg cgaggagctg ccttatgcga
     4321 tgaacgtcga agtggagcag tttgaagagg gagacggttt gaaccgcatc tacatcgccg
     4381 ttttggtcga caaagaaagc caaaaggcga ttttgatcgg taaaggcggg gagcgtttga
     4441 aaaaaatttc caccgaagcg cggctggata tggaaaaact gtttgataac aaagtatttt
     4501 tgaaggtctg ggtcaaagtc aaatccggtt gggcagacga cattcgcttc ctgcgcgagc
     4561 tgggtttgta gtttttcttg ctgaacttta cgcaaatgcc gtctgaacag gtttcggacg
     4621 gcattttgtt tcaatcggga atatctttgt taaaaacggg ttgata
//
LOCUS       DAWTCI010000108         4553 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWTCI010000108 DAWTCI010000000
VERSION     DAWTCI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.108"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(38..763)
                     /locus_tag="WM481_001991"
     CDS             complement(38..763)
                     /locus_tag="WM481_001991"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFA9081225.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1336)
                     /gene="fur"
                     /locus_tag="WM481_001992"
     CDS             complement(833..1336)
                     /gene="fur"
                     /locus_tag="WM481_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFA9081226.1"
                     /translation="MIVGKQAEIAYYSVYYFTGILNIMEKFSNIAQLKDSGLKVTGPR
                     LKILDLFEKHAEEHLSAEDVYRILLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGK
                     AVYELDKGDHHDHIVCVKCGEVTEFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSD
                     CQAKGKR"
     gene            1494..1871
                     /locus_tag="WM481_001993"
     CDS             1494..1871
                     /locus_tag="WM481_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFA9081227.1"
                     /translation="MNKTLILALSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALRAKQNADKQ"
     gene            1881..2690
                     /gene="dapB"
                     /locus_tag="WM481_001994"
     CDS             1881..2690
                     /gene="dapB"
                     /locus_tag="WM481_001994"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFA9081228.1"
                     /translation="MIPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIRAAAEKTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2804..3118)
                     /locus_tag="WM481_001995"
     CDS             complement(2804..3118)
                     /locus_tag="WM481_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081229.1"
                     /translation="MFHLFFVNFYFRQLCPTPNYTDWVKIKFKQFSYLKFIYGYATEN
                     QDKDIDNTLELGELKQDDEILDYGGALALIRRRYNLPTSFIIDIVCREIELEFLDQES
                     FN"
     gene            complement(3164..3322)
                     /locus_tag="WM481_001996"
     CDS             complement(3164..3322)
                     /locus_tag="WM481_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081230.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGSLTD"
     gene            complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="WM481_001997"
     CDS             complement(3330..4502)
                     /gene="lpxB"
                     /locus_tag="WM481_001997"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFA9081231.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARL
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRELVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARKTLGADVGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLALTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFGALHLLLKKDTADLAARAVLEEAGC"
BASE COUNT         1132 a         1469 c         1005 g          947 t
ORIGIN      
        1 ccactatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgta tcctgctggc
      181 agtctatcag ttccacgccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacaccctg ccgatctgaa
      301 cgccgtaaaa gccgcccgcc aacctcgttt taccgttttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagtccc atcctgattc gggcgcgccg ctgccgcaca atgcgcgaca acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttacgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaattaccg cacgcggccc gaccgcatac caaaaaaacc
      601 aaccgtcccg cgaaaaccaa ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 cgtccaaatc cctgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcggcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcgaact
     1081 gcgtcagcac gcggtaaatc gtcgccacac cgatttccac gccctcttcc agcagaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcgtgctt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgcgcaatg ttgctgaatt
     1261 tttccataat attcaatatc cctgtaaaat aatagacgct ataatacgca atttcagcct
     1321 gcttgcccac tatcacacca tagcagttcg caatagcaaa accgcaacgg cggacgggca
     1381 gtcggacaca gacagggtaa aataccgctt aagcgtatga ttatcgttcg cttttataaa
     1441 atattcaagc agtgctacac tagacatccc gatttgcaca gaaaggttct cccgtgaaca
     1501 aaaccctcat cctcgccctt tccgccctgt tcagcctgac cgcgtgcagc gtcgaacgcg
     1561 tctcgctgtt tccctcctac aaactcaaaa tcatccaagg caacgaactc gaaccgcgcg
     1621 ccgttgccgc cctgcgcccc ggcatgacca aagaccaagt cctgctcctg ctcggcagcc
     1681 ccatactgcg cgacgctttc cataccgacc gctgggacta taccttcaac acctcccgca
     1741 acggcatcat caaagaacgc agcaacctga ccgtctattt tgaaaacggc gtactcgtcc
     1801 gcaccgaagg cgacgccctc caaaatgccg ccgaagccct ccgcgcgaaa caaaacgcag
     1861 acaaacaata aggaacacac atgataccgc tcaaaatcgc catcgccggc gcaaacggcc
     1921 gcatgggacg cgtcctcgtc gaagccgtca acaaccatcc cgacaccgtc ctttccggcg
     1981 cgctcgaaca ctcaggctca gaagccctcg ggctggacgc gggctacgcc gtcggactca
     2041 aaaccggcat cgccatttca gacgacgttg acgccgttct cgcccaaagc gacgtactca
     2101 tcgacttcac ccgccccgag ccgaccctca agcacctgca aaaatgcgtt gaaaaacaag
     2161 tcaacatcat catcggcaca acaggcttcg acgatgcggg caaagccgcc atccgcgccg
     2221 ccgccgaaaa aacaggcatc gttttcgccg ccaacttcag cgtcggcgtc aacctcacct
     2281 tccacatcct cgacaccgtc gcccgcgtcc tcaacgaagg ctacgacatc gaaatcatcg
     2341 aaggccacca ccgccacaaa gtcgatgccc ccagcggcac agcattacga atgggcgaag
     2401 tcatcgccgg cgcgctcggc cgcgacctca aacaatgcgc cgtttacggc cgcgaaggcc
     2461 acactggtcc gcgcgatcct tccaccatcg gctttgccac cgtccgcgca ggcgacatag
     2521 tcggcgacca caccgccctc ttcgccaccg acggcgaacg cgtggaaatc acccacaaag
     2581 ccggcagccg catgaccttt gccgccggtg ccgtccgcgc cgcagtttgg gtaaacggca
     2641 aaacgggttt gtacgatatg caggacgtac tcgggctgaa caaccgttaa cccccataca
     2701 aaatgccgtc tgaaaagata ttgttcagac ggcattttgc cgacaggctt cgtatcggca
     2761 tatcaataaa gttgcttgtg cagcatgaca agctacggct cgtttaattg aaactctcct
     2821 gatctaaaaa ttccaactct atttcccggc aaactatatc tatgataaaa ctggtcggaa
     2881 gattatacct acgccttatt aatgccagtg cacctccata atccaagatt tcatcatcct
     2941 gctttaattc tccaagctcc aaggtattat cgatatcttt atcttggttc tccgtagcgt
     3001 atccatagat aaatttcaaa tagctgaact gcttgaattt aatttttacc cagtctgtgt
     3061 aatttggtgt aggacacaat tgcctaaaat agaaatttac gaagaacaaa tgaaacaagg
     3121 tacgtaagcg tattagtata aactataatc atatataata aaattaatca gttaacgaac
     3181 cattatattt taaccttaat cccattcggg ccagttctgt ttcatttgga attcctctta
     3241 aatacttatt gtttgatgtg ccattaatca caatttctcc cccttttttt gtaatacgtg
     3301 ctgcttcggg aaaataatcc attaaccgct caacatcccg cctcttccaa aaccgcacgc
     3361 gcggccaaat ccgccgtatc ttttttcaac agcaggtgca acgccccgaa atcctgttgc
     3421 agcgcggcaa ccttatcggg gtgttcgtac cagtccgcca atgccgccgc cagtttttcc
     3481 ggttttgctt cagattgcaa taattccggc acggcctcct tacccaacag gatattcggc
     3541 aggccgacat gcggcacttt gattttgcgt ttcacataag cataggtcag cggcgaaatc
     3601 ttgtagctga tgaccatcgg acgcttgcac aacgccacct ccaaagtcgc cgtaccgctc
     3661 gttaccagca ccgcatccgc cgccctgcac accgtttcag actgtctgtc ggttaccgtc
     3721 agcgccaatc cggcaaactc cggccgctgc aaaacttccg ccaaacgccg cttcgtcgcc
     3781 tccgttgcgg caggcagcag gaagcgggcg gcgggatagc gttccaacaa caataatgcc
     3841 gtctgaaaaa acaccggcgc catatagtcg atttcgctga cgcggctgcc gggcagcagg
     3901 gcgaatacgg ggatgccgac atccgcgccc aaagttttcc gcgccgtttc acggtcgtct
     3961 tccaagggca taagctgtgc catcggatga ccgacaaact ccgcacgtcc gcccgcatcg
     4021 agataaagct gcggctccat cgggaacagg cacagcacgc ggttgacctg atgcacgatt
     4081 ttgcccacgc gttcgcgccg ccacgcccaa accgacggac tgacataatg cagggtcgga
     4141 atgcccgccc gtttcagctt ttccgccaca cccagattaa aatcgggcgc atcgataccg
     4201 acaaagacat caggtttcaa cgacagcaaa tcccgtacca gctccctgcg tatccgtaaa
     4261 atttccggca gccgcctgac cacttcgaca aagccgcgca ccgccagccg ctcctgatca
     4321 taaaggctct cgaaaccttc cgccttcatc agttccccgc cgatgccggt taaccgtgcc
     4381 tgcgggcaac gctttcggat ggcgcgtatc aggtgcgccc ccaacaggtc gcccgacgct
     4441 tcgccgacac tgacggcaat caaagggctt tttttatcaa ccatattcgt ctgtccccac
     4501 atatagtgga ttaacaaaaa ccggtacggc gttgcctcgc cttgccgtac tat
//
LOCUS       DAWTCI010000109         4390 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWTCI010000109 DAWTCI010000000
VERSION     DAWTCI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.109"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..1212)
                     /locus_tag="WM481_001998"
     CDS             complement(<1..1212)
                     /locus_tag="WM481_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081232.1"
                     /translation="MKQKKTVQCILLGFAAASMHAQGAAAANSGTIEKTDKYTLVLAK
                     QGQENNYTLNGGTEVKPLNSLIIAANGGTNNITIKGKLADGPADAPPTIDNNSIERNI
                     NKNGYTYAWQNWSGAVMLVDQSYEGENKVTFENVTIAAHNAPAGILSDDRHKSSSLAP
                     AMLAFKGRNTINMDADSNANSSNEGILLLNNGEKMGEYRLVSEEGSTLNINIKSGKDK
                     GQGITANHYGNSDINFNKASPNITTMEFKGDVNIKIDRNGQEEAESNGFGFYSSRKLG
                     NKKQIPEGSKMEAIFRGNVDIVATPVYDEQGRPKSIGSAFAIDGKYSKVEVVGGEGKV
                     VKIKGDIFAYNGGSVSVNLANKDSYFEGEAHIGKRSFAKGKDMFALTVDADGYELTPD
                     TKSIEKKKKELN"
     gene            1409..1484
                     /locus_tag="WM481_001999"
     tRNA            1409..1484
                     /locus_tag="WM481_001999"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Glu,seq:ttc)
     gene            complement(1700..1852)
                     /locus_tag="WM481_002000"
     CDS             complement(1700..1852)
                     /locus_tag="WM481_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081233.1"
                     /translation="MPSENRSAGFVVHYYVVLQDKAQGKAAGCIVATDNEKRGRCRLN
                     RVLRRV"
     gene            1912..2718
                     /gene="thiD"
                     /locus_tag="WM481_002001"
     CDS             1912..2718
                     /gene="thiD"
                     /locus_tag="WM481_002001"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFA9081234.1"
                     /translation="MKGSFVQTLAIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCSFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDILTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVIIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="WM481_002002"
     CDS             complement(2776..3645)
                     /gene="tehB"
                     /locus_tag="WM481_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFA9081235.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRAPDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADCNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVMMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPG"
     gene            3758..4195
                     /gene="rnhA"
                     /locus_tag="WM481_002003"
     CDS             3758..4195
                     /gene="rnhA"
                     /locus_tag="WM481_002003"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFA9081236.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWQELDALVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT         1012 a         1234 c         1038 g         1106 t
ORIGIN      
        1 attcagctct ttctttttct tttcaatcga ctttgtatcg ggggttaatt catatccgtc
       61 cgcatccacg gttaaggcaa acatatcttt ccctttggca aagctccttt tcccgatatg
      121 ggcttcccct tcaaaataag agtctttgtt ggcaaggttc acgctcacgc tgccgccgtt
      181 gtaggcgaaa atatcgcctt tgattttgac aaccttgcct tccccgccga caacctcgac
      241 tttgctgtat ttcccgtcaa tcgcaaacgc gctgcctatg cttttgggcc tgccttgctc
      301 gtcataaacc ggcgttgcaa cgatatcgac gttcccacgg aaaatcgctt ccatcttaga
      361 accttccgga atctgcttct tattgcctaa tttgcggctt gaatagaagc caaaaccgtt
      421 gctttccgcc tcttcctgtc cgttcctatc gattttaatg tttacatcgc ccttgaactc
      481 cattgtggtg atgtttggag aagccttgtt gaagttgatg tctgagttac catagtggtt
      541 ggcggtaatg ccctgtcctt tatcttttcc tgatttgatg ttgatattca gcgtcgagcc
      601 ttcttcggaa acaagacgat attctcccat tttttcacca ttattaagca ataggatacc
      661 ttcgttacta gagttggcat ttgagtccgc gtccatattg atggtattgc gccctttaaa
      721 ggcaagcatc gccggcgcca agctactgga tttatgccta tcatcagaca aaatgccggc
      781 aggcgcgtta tgggcggcta tggttacgtt ttcaaaagta accttatttt ctccctcata
      841 gctttgatcc accaacatca ccgcgccgct ccaattctgc catgcgtacg tgtaaccgtt
      901 tttatttata tttctttcaa tcgagttatt gtcaatcgtc ggcggggcat cggcaggacc
      961 gtctgccaat ttgcctttta tcgtaatgtt gttcgtaccg ccgttggcgg caatgatgag
     1021 ggaatttaag ggtttgactt cggttccgcc attaagggta tagttgttct cttgcccttg
     1081 ttttgcgagg acgagggtgt atttgtctgt cttctctatc gttccactgt ttgcagcggc
     1141 cgccccttgc gcgtgcatag aagcggccgc gaagccaagt aagatgcact gaactgtctt
     1201 cttttgtttc atcggttttt ccattttgaa ggtatgtgat ttttatcatt atatgcttaa
     1261 atcattgaca cgcccccaaa aaaaatgaat tgcttgatat tccaatgttt tttatcttgt
     1321 gatacggtca gacaaacgcc ccctgacatt cgtttagacg gcatcgtatt gctaaatttc
     1381 tataagtatg tataatgtcc gtttccacgc gcccatcgtc tagaggccta ggacactgcc
     1441 ctttcacggc ggcaaccggg gttcgaatcc ccgtgggcgt gccaattcaa aaacctgctt
     1501 gtttcaagca ggttttttat tatgaatcgt tattcccgca atttttcgtc attgccgtga
     1561 aaacgggaat ctagaacctc aaactttcag ataatctttg aatattgctg ttgttctaag
     1621 gtccggattc ccgcctgcgc gggaatgacg gaattttagg tttctgtttt tgcgggaatg
     1681 acgaatgaag cgtgccggtt tatactcgcc gcaacacgcg attcagacgg catcgccctc
     1741 ttttttcatt atcagttgct acgatgcacc ccgctgcctt gccctgtgcc ttgtcctgca
     1801 atacgacata ataatgcacc acaaaccccg cgctgcggtt ttcagacggc attgcctgct
     1861 tttttacagg cagtagccct ttttatcgga cgcaatatta aggaggaaca aatgaaaggc
     1921 tcttttgtgc agacgcttgc catcgccggt tcggattcgg gcggcggtgc gggcattcag
     1981 gcggatttga aaacgtttca gatgcgcggc gtgttcggaa cgtgtgtcat caccgccgtt
     2041 accgcgcaaa ataccttggg cgtgtcggcg gttcatctcg tcccgaccga aaccatcacc
     2101 gcacaaatcc aagcaatcag ggaagacttt gacatccgcg cctacaaaat cggtatgctc
     2161 ggcacggcgg aaatcatcga atgcgttgcc gacaagctga aacactgcag ctttggcagg
     2221 cgcgtactcg accctgtgat gattgccaaa ggcggtgcgc cgctgttgca ggattccgcc
     2281 gttgcggcac tgacgcgcct gctgctgccg gacacggaca tcctgacccc caacctgccc
     2341 gaggcggaag ccttgacggg cgtgcatatc gaaaaccgta aagatgcgga acgtgcggca
     2401 aaaatcctgc ttgattacgg tgtcaaaaat gtcattatca aaggcggaca tttgaacggc
     2461 agcacaagcg gacgctgcac ggattggctg tttacgcaaa acgaaacgct ggaattggac
     2521 agcccgcgct ttccgaccgc ccacacgcac ggcacgggct gcacgttttc cgcctgcatt
     2581 accgccgagt tggcaaaagg cttagacgtt tgcaaagccg tacagactgc caaggcctac
     2641 atcacggcgg caatctcaaa ccctttggaa atcggcgcgg gacacgggcc ggtcaatcat
     2701 tgggcgtatc gggactagac gtaaaaatgc cgtctgaacg ccttttcggg ttcagacggc
     2761 atcgtccggc gcggcttatc ctggtttttt cgccagcata gttacaaatt taaaccgtat
     2821 cgggttgccg ttttcgtctt tggcgtgcat cgcgcccaat tcttctttat attcgaccag
     2881 ctcccaatcc cgataataat ccttcagctc gccctcttta aatttgaagg ggaacggcat
     2941 cgggcagggg aaatccgccg tatccattgc cgatacgatt aagttgtacc cgcccgccgc
     3001 cgtatgcgcc tgcatatcgg caatcacgtc gggcacgcgc tgcggcatca ggaacatcat
     3061 caccaccgtt gccacaatat aatcaaactc gccctgaagg gcggcggcgt tcaaatcata
     3121 ttccagcgtg cggacgttca aaccctccgc ctctgccagt tccgccacgt ttgccaacgc
     3181 ggcgggattg caatcggcag cggttacatc aaaccccttc aaacccagga acagcgcgtt
     3241 gcgcccctgt ccgcagccca tatccaacgc cctgcccgcc ggtacggtat cctgtgccgc
     3301 cgcgaccgca gaatgcgtgg cactcatccc gtattttttg tgaaaatagt ctgccgccgc
     3361 gcaatacagc gacaaacgga tttcggcatc gtccgttttc ggtttgaccg aaaacacctg
     3421 ctgcggcgca aacacgcaat cgccgctttc cgccgaccaa acctctgccg ccccgtccgg
     3481 tgcacggact tcgacatcgc cctgcaacac attcaggcag acccattccc cttcctcgga
     3541 cgaataaccc gacaacaaaa cttccggcag gttttccact ttccatacag gcatttgtcc
     3601 gaaacaaaac aactcgccac tttgacccac tatccgctcc ttcatattca aaaataaagt
     3661 tgcacattat atgcctattt caatccgccg caatctttca gacggcacga cgcacaaacc
     3721 gcttataatc acgccggaca ccacacaaag gcacgatatg gacacacccg tttacctcta
     3781 cacggacggc gcgtgcaaag gcaatcccgg cgcgggcggc tggggcgtat taatgcgcta
     3841 cggcagccgc gaaaaagaac ttttcggcgg cgaagcgcaa accaccaaca accgcatgga
     3901 gctgaccgcc gttatcgaag ggctgaaatc gctcaaacgc cgctgcaccg tcatcatctg
     3961 caccgactcg caatacgtca aaaacggcat ggaaaactgg atacacggtt ggaagcgcaa
     4021 cggctggaaa accgccgcca aacagcccgt caaaaacgac gacttgtggc aagaactcga
     4081 cgctctggtc gggcagcatc aagtcagctg gacttgggtt aaaggacacg cgggacacgc
     4141 ggaaaacgaa cgcgccgacg atttggcaaa ccgtggcgcg gcgcagtttt cttgattgcc
     4201 gcaccgacaa aaatgccgtc tgaaaccgct aatgggcttc agacggcatc gtcctccacc
     4261 gtcattcccg cgcaggcggg aatccagaac gtcgggcaac ggcaatattc aaaagccgtc
     4321 tgaaaattta aaagttctag attcccgttt tcacgggaat gacgaaaagt tgcggacagc
     4381 agaggaatga
//
LOCUS       DAWTCI010000110         4334 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWTCI010000110 DAWTCI010000000
VERSION     DAWTCI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.110"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            372..1292
                     /gene="ribF"
                     /locus_tag="WM481_002004"
     CDS             372..1292
                     /gene="ribF"
                     /locus_tag="WM481_002004"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFA9081237.1"
                     /translation="MKIRPGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAARTGKTPPCRISPLRTKLGLLEKTGCVDAAWVLRFDRNF
                     SEISAQAFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLGGRVVHGRKLGRTLNAPTANIRLPGHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDGGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRRIEADMEAAKCW"
     gene            1434..4223
                     /gene="ileS"
                     /locus_tag="WM481_002005"
     CDS             1434..4223
                     /gene="ileS"
                     /locus_tag="WM481_002005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFA9081238.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDNPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKNTVA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     GALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWPEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGSDGKTLRDKAIKAVDDTEFFPPWG
                     RARLESMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGGYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PAINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1048 a         1225 c         1163 g          898 t
ORIGIN      
        1 gattttcact gatgcttcaa aggcggatta tatcgggttc cgggcggtgt ttcaatacat
       61 agcaccgcgc ctgctgcgcg ttttatgcgt ttggcgcgtt cggcggcggg aaatttgcct
      121 acttttcccg cgtcgggcgg gcggaacggg cggcacactg tctataaacc gcaataccgt
      181 ttacaatgac cgcctgtttc accacatacc cgaacgcaac aatgtttcaa cacacgggac
      241 ggcacatcaa gcaccgccct atgtgtcgtc ctgatttgga aggggttgca cccctcccga
      301 ataaagtctg atcctgccgc cccgaaggac ggatgtccga gtggcggggt ttcaaccgaa
      361 aaggaaatac aatgaaaatc aggccggggc ggcacaacgc gcccgacttt ccgcacgggg
      421 ccgccgtaac cataggcaat ttcgacggcg tgcacctcgg acacaaacac atcctccaaa
      481 aactccgcct cgaagccgac gcacgcggat tgcccgtcgt tgccgtcatt ttcgagccgc
      541 aacccaaaga attttttgcc gcccgcacag gcaaaacccc gccgtgccgt atcagccccc
      601 tgcgtaccaa actcgggctg ctcgaaaaaa ccggttgcgt cgatgccgcc tgggttttgc
      661 gtttcgatcg gaatttttcc gaaatatccg cgcaagcatt tatcgaccgc ctgctgcgtc
      721 aaaccttgaa tacgcgctat ttgctcgtcg gcgatgattt ccgtttcggt gcggggcggg
      781 aaggctgttt tgaacttttg gcacaacagc ccgatatgca aaccgagcgc acgccttccg
      841 tcatcgtcga agacatccgc accagcagta ccgccgtccg ccaagccctt tcagacggca
      901 accttgccta tgcgaagaaa cttttggggc acgactacgt tttggggggc agggtggtgc
      961 acggcagaaa actcggacgc accttaaacg ccccgaccgc caatatccgc ctgcccggcc
     1021 accgttatgc actcggcggc gtgttcgtcg tcgaggcgga cggcgcattc ggcacgcggc
     1081 gcggcgtggc gagcttcggc ttcaatccca ccgttgatgg cggctgttct caaaagcttg
     1141 aagtccacct gttcgacttt caaggcgatt tgtacggaca acggttgaac gtccgcttcc
     1201 tgcacaaact gcgcgacgag gaaaagtttg acggtatgga agaactgaaa aggcggattg
     1261 aagccgatat ggaagccgca aagtgttggt agaaaaacct tatacaaacc atccgattgg
     1321 gctacaatca gccttttaac tgttcggacg gcacaggggt ttcccgttgt gaaatactgt
     1381 ttgaggcgca atgccgtctg aaaccgaaat attgtaacaa tagagattaa aaaatgaccg
     1441 attacagtaa aaccgtcaac ctgctcgaaa gcccgtttcc gatgcgcggc aatcttgcca
     1501 agcgcgaacc tgcgtggctg aaaagctggt acgagcaaaa acgttaccaa aaactgcgcg
     1561 aaatcgccaa aggccgtccg aaattcattc tgcacgacgg cccgccgtat gccaacggcg
     1621 acatccatat cggtcatgcc gtcaataaaa ttcttaaaga cattattatc cgcagcaaaa
     1681 cccaagccgg ttttgacgcg ccttatgtac cgggttggga ctgccacggt ttgcctatcg
     1741 aagtaatggt ggaaaaactg cacggcaaag atatgcctaa agcccgtttc cgcgaattgt
     1801 gccgcgaata tgccgccgaa cagattgccc gtcagaaaaa agactttatc cgcttgggcg
     1861 tgttgggcga ctgggacaat ccttacttga ccatggattt caaaaccgaa gccgataccg
     1921 tgcgtatgct cggcgaaatc tacaaatccg gctatctcta ccgtggtgcg aaaccggttc
     1981 agttttgctt ggattgcgga tcttcgctgg cggaagcgga agtggaatac aaagacaaag
     2041 tatcgcctgc gattgacgtt gcctatccgt ttaaaaacac cgtcgcgctt gccgccgcat
     2101 tcggcttggc aggtatcgaa ggcaaagcgt ttgccgtcat ttggacgacc acgccttgga
     2161 ctctgcctgc gagccaggcc gtgtctgccg gcgcggacgt ggtgtatcaa ttaatcgata
     2221 cgcccaaagg caaattggtg ctggcgaaag atttggcgga aggcgctttg aaacgctacg
     2281 gcttttcaga cggcatcgcc atccttgccg aaaccaccgg cgacaagctg gaaaacctgc
     2341 acatgaatca tccgttcctc gaacgcgata ttcccatgct caacggcgaa cacgttacca
     2401 ccgatgccgg taccggcttg gtgcatactg cgcctgcgca cggtttggaa gactacgccg
     2461 tctgcaataa atacggcatc gagctttaca accctgtcaa cgccgaaggc aaatacataa
     2521 gcgaaacgcc tcgtgtcgca ggcatgagcg tttgggaggc gaatcccgtc atcctgcaat
     2581 ggccggaaga aaccggcaac ctcttggcaa gcagcaaaat cgaacacagc tacgcccact
     2641 gctggcgcca caaaaccccg ctgatttacc gagcgacagg tcagtggttt gtcggcatgg
     2701 acaaagccgg cagcgacggt aaaaccctgc gcgacaaagc catcaaagcc gtggacgaca
     2761 ccgaattctt cccgccatgg ggtcgtgcgc gtttggaatc catgattgaa ggccgtcctg
     2821 actgggtggt ttcacgccaa cgctattggg gcacgccgat gactttcttt gttcacaaag
     2881 aaacgggcga gctgcatccg aactctgccg aacttttgga aaaagtcgcg caacgcatcg
     2941 aagaaaaagg catcgaggct tggttctccc tcgataaaag cgaattatta agcgcggaag
     3001 attgcgaaca ttacgacaaa ctccccgata ccatggacgt atggttcgac tcaggctcga
     3061 cgcattattc cgttgtaaaa caacgcgaag aattggaatg gccggctgac ttgtacctcg
     3121 aaggcagcga ccaacaccgc ggctggttcc aatcctctat gctgaccggt tgcgcctcat
     3181 ccatgggacg cgcaccgtat aaacagctgc tgacccacgg tttcgtggtt gaccaaaacg
     3241 gccgcaaaat gtcgaaatcc atcggcaacg tcgtcgcgcc gcaggaagtc tataacgaat
     3301 tcggcgcgga catcctgcgc ctgtgggcgg catccaccga ttacagcggc gaattggcga
     3361 tttccaaaga aatcctcaaa cgcgtaaccg aaagctaccg ccgcatccgc aataccttga
     3421 gcttcctgtt tgccaacttg agcgatttca acccgattga agatgccgtg caacaggcgg
     3481 atatggtgga aatcgaccgc tacgccttgg tattggcgcg gcggctgcaa gagcgtttgg
     3541 caggcggtta ctatccgcgc tatgccttcc acttcgccgt gaaagacatt gtttctttct
     3601 gctcggaaga cttgggcgcg ttctacctcg acatcctgaa agaccgcctc tacaccacca
     3661 aagccgacag ccgcgcccgc cgcagcgcgc aaaccgccct gtaccacatc acgcgcagcc
     3721 tggttctctt gattgcaccg attttgtgct tcaccggcga agaagcgtgg gacatcatcg
     3781 gcggcggcga agaagacagc gtcctcttcc atacttggca cgagttcccg gccatcaacg
     3841 aaaaagccga agccgaactg gtgaaaaaat ggacggcaat ccgcgaagcg cgcgaagcag
     3901 taaccgccgc catcgagcct ttgcgcgccg acaaaaccgt cggttcgtcc ttgcaggcgg
     3961 aagccgaaat taccgcgccg gaagaaatgg ccggctatct gaatgctttg ggcgaagaat
     4021 tgcgctttgc cctgctggtg tctaaagcag aagtgaaagt cggtgatgaa cttgccgttg
     4081 ccgccaaagc cagcgacggc gaaaaatgcg aacgctgctg gcattacacc cgcgatgtgg
     4141 gcgcggttgc aggttatgaa accgtctgca aacgctgtgc ggagaatgtc ggcggagaag
     4201 gcgaaacgcg ccattacgcc tgataaagtt tgagcaaatg ccgtctgaaa ccgccaaaca
     4261 gtatttcaga cggcattttt tgtgccgcga tttgtcttca taatggcgga ggggtttcga
     4321 gattgcggtg ttgt
//
LOCUS       DAWTCI010000111         4207 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWTCI010000111 DAWTCI010000000
VERSION     DAWTCI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.111"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            37..228
                     /gene="mobC"
                     /locus_tag="WM481_002006"
     CDS             37..228
                     /gene="mobC"
                     /locus_tag="WM481_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFA9081239.1"
                     /translation="MRVLAGMGNNLNQIAKALNTAAKVGTLGNVEALKATTELAALER
                     SLNSLRDFLAKEKNGWQSQ"
     gene            213..842
                     /locus_tag="WM481_002007"
     CDS             213..842
                     /locus_tag="WM481_002007"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFA9081240.1"
                     /translation="MAVPMIVQFFNRGKGGGSGPIDYLLGKDRDREEARLLRGDPEET
                     AALINSSDYAKKYTAGCLSFEESNIPAEQKHALMDSFEECIFAGLDKDQYNCLWVEHR
                     DKGRLELNFVIPNIELLSGKRLQPYYYAADRGRVDAWRTMQNLTHGYSDPDDPAKRQS
                     MTQAKDLPRNTQEAAQSITEHHRRLRSPSPIRQAKKPRRRAGNAGKGRF"
     gene            926..1567
                     /locus_tag="WM481_002008"
     CDS             926..1567
                     /locus_tag="WM481_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein B"
                     /protein_id="HFA9081241.1"
                     /translation="MSKISDLAKTFEQTSRTEAASIEQQTKADLETLRKNINEALKQS
                     EQKITADINARQLRMSKAVFKPYLWSLLGISAAGLIVIAGLFIAIWSVKNELDDLKQQ
                     RAEAERTLDLLETKTKGLTLENCPVENSKATRVCVATEKRMLDALAELESNHAAIEQR
                     MMKALTHLGERLAELEQENTSLAQQLASLAAELERQSEIQQRQSEILNQLAKR"
     gene            1599..1868
                     /locus_tag="WM481_002009"
     CDS             1599..1868
                     /locus_tag="WM481_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic plasmid protein A"
                     /protein_id="HFA9081242.1"
                     /translation="MGNEPMITENERDRRTAAWLIETYGAEAVAEAETRIAGARKPYP
                     SNIAKVLGASLPEALKRTENAAARQKLAGLRRMLDGKAVKTSQDL"
     gene            complement(1846..2124)
                     /locus_tag="WM481_002010"
     CDS             complement(1846..2124)
                     /locus_tag="WM481_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease VapD"
                     /protein_id="HFA9081243.1"
                     /translation="MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSL
                     YTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRIEQWSDFTSLVKS"
     gene            complement(2124..2309)
                     /locus_tag="WM481_002011"
     CDS             complement(2124..2309)
                     /locus_tag="WM481_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFA9081244.1"
                     /translation="MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESG
                     EHTQYKLSHIVQDPKAA"
     gene            complement(2312..2557)
                     /locus_tag="WM481_002012"
     CDS             complement(2312..2557)
                     /locus_tag="WM481_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081245.1"
                     /translation="MTRYAINRDALYSAFKDFLYSEINANPALKSATVDDLADIVLAK
                     KWRIFLPDGIKRTTAEKAAQRVLYMTNQPENQTQQEN"
     gene            complement(2554..2796)
                     /locus_tag="WM481_002013"
     CDS             complement(2554..2796)
                     /locus_tag="WM481_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFA9081246.1"
                     /translation="MAYPKLKTTKRDVTAKELAKRFGCSTRTVFRAWSQSRADYLAEN
                     SISRDKPWEHFGISRATWYRRGKPMPSETDNQSETA"
     gene            complement(2800..3582)
                     /locus_tag="WM481_002014"
     CDS             complement(2800..3582)
                     /locus_tag="WM481_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFA9081247.1"
                     /translation="MLVRSYAAAIKRRYIQVNPPHLRVFMLFDLDYEGAGLAWEDNNL
                     PMPAWAAINRENGGAHLAYALSAPVLTAEYGGRQKALRYLAALEAAYKAKLRGDVGFV
                     SLITKNPEHPHWLTLRGVPDAIRGYDLEYLADFVDLDKFKPYIGRSNVEAVGLSRNCT
                     VFNLVSRWAHKNVLAFKQQGYTVQGWLKEVHYQCMRVNGDFPVPMWEKEVKCISKSIA
                     NWVWYKFDIAASNRRFSELQAHRNSLRKTTINAGRTKIITEL"
     gene            complement(3752..4150)
                     /locus_tag="WM481_002015"
     CDS             complement(3752..4150)
                     /locus_tag="WM481_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081248.1"
                     /translation="MGLLSGQSLKLGRADSDDELLCSLFGGTRTCQKGKIPLFLTAFC
                     SKKRHFITAFRRYSMKNSGKNTHAALSELVSCFPQNAALLAGLGRAAALQALPLRLLA
                     SLNCGLENFEIRTPIGLLSDYGYFDIVVSS"
BASE COUNT         1144 a         1039 c         1129 g          895 t
ORIGIN      
        1 aaaaaaaccg tcaaattcca attcccgccc gaagtcgtgc gcgtccttgc aggcatgggt
       61 aacaacctga accaaatagc caaggccctg aacaccgccg caaaggtcgg cacgttgggc
      121 aatgtggaag cactcaaggc gacgaccgag ctggcagcgt tggaacgttc cttaaattcc
      181 ctacgggatt ttttagccaa agaaaagaac ggatggcagt cccaatgatt gtgcagtttt
      241 tcaatagggg gaaaggcggc gggagtggtc cgatagacta tcttctaggc aaagaccgcg
      301 accgagaaga agccagatta ttacgcggcg accccgaaga aaccgccgcc ctgataaaca
      361 gcagcgatta cgccaagaaa tacaccgccg gctgcctgag ctttgaagaa agcaacatcc
      421 ccgccgaaca gaaacacgcc ctgatggaca gcttcgaaga gtgtattttt gcaggcttgg
      481 acaaagacca atacaactgc ctatgggtag aacaccgaga caaagggcgt ttggaactca
      541 acttcgtgat accgaacatc gagcttttga gcggaaagcg gttacagccc tactactacg
      601 ccgccgacag aggaagagtg gacgcatggc gcaccatgca gaacctgacg cacggataca
      661 gcgacccaga cgaccccgcc aaacggcaga gcatgaccca agccaaagac ctgccgagaa
      721 acacgcagga agccgcacag agcatcacag agcatcacag acggcttaga agccctagcc
      781 ctatcaggca agctaaaaag ccgcgcagac gtgctggaaa cgctggaaaa ggtaggtttt
      841 gaaatatcac gagcgaccat cagcagcatc agcatcaaga acccggaccc aaaagggcgc
      901 aacatccgac tgaaaggcgc actgtatgag caagatttcc gatttggcga agaccttcga
      961 gcagacatca cgcaccgaag ccgccagcat agagcaacaa acgaaagcag acttagagac
     1021 gttacggaaa aatatcaacg aggcattgaa gcaaagcgag cagaaaataa ccgccgatat
     1081 aaacgcccgg cagttacgca tgagcaaggc agtattcaag ccctatctgt ggagcttgct
     1141 aggtatatcg gcggcagggt tgatagtcat agcagggctg ttcatagcga tatggagcgt
     1201 caagaacgag ctggacgact tgaaacagca gagagccgaa gcagagcgca ccctagacct
     1261 gttggaaacc aagaccaaag gtttgacact ggaaaattgc ccagtcgaga acagcaaagc
     1321 aacgcgggta tgcgtagcga ccgagaagcg aatgctggac gcgttagcgg aattagagag
     1381 caatcacgca gcaatcgagc agcgaatgat gaaagcctta acgcacttgg gcgaaaggtt
     1441 ggcagagcta gagcaggaaa acacgagttt agcgcagcag ctagcgagct tggcagccga
     1501 gttagagcgg cagagcgaaa tacagcaacg gcagagcgaa atcttgaatc aactagccaa
     1561 acgataagcc aacgacacaa acgaacccaa agcaggggat ggggaatgag ccgatgatta
     1621 ccgagaacga acgcgacagg cgaacagccg catggctgat agagacctac ggggcagaag
     1681 ccgtagcgga agcagaaacc cgcattgcgg gcgcgagaaa gccctatccg agcaatatcg
     1741 ccaaagtatt gggggctagc ctacccgaag ccctaaaacg cacagaaaac gccgcagcgc
     1801 gccaaaaact ggcagggctg cggcggatgt tggacggtaa ggcagttaag acttcacaag
     1861 acttgtgaaa tcagaccact gctcaatgcg gaacgcccga atatcgcgga cagaagacgg
     1921 aaaccaaggc agagctttta gctcgttgat ggctgaaaac aggttagcca tatcttcgtt
     1981 ttggcaggtg tacaaacttc cctgaatgcg ggtaaagccg aatttccgca gggtgtatcc
     2041 aatatccgcg taggcttggg aaatgccttt cggatggttt tgagcggtat cggcaaccac
     2101 caaatcaaaa gaaatcgcgt acattaggca gcctttgggt cttgaacgat gtgggacagt
     2161 ttgtattgcg tgtgttcgcc gctttccggc agttcaatat ttacgagcca atcgccgtcc
     2221 ggaagctgtt cggcaggcgt gccgacgatg tacatcacgc cgtaatcgcc aaaggtgcgg
     2281 attgaaccgg tggggatagt ggtttgcatg gttagttctc ctgttgggtt tggttttcag
     2341 gctggtttgt catgtacaaa acccgttggg cagctttttc ggctgttgtg cgtttgatgc
     2401 cgtcaggcag aaaaattctc cattttttgg ctagaactat atctgcaaga tcatcaaccg
     2461 ttgcgctttt caaagcagga tttgcgttaa tttccgaata cagaaaatct ttgaatgccg
     2521 agtacagagc atcgcggttt attgcgtatc ttgtcatgct gtttcgcttt ggttgtcagt
     2581 ttcagacggc ataggcttgc cgcgcctgta ccaagtggcg cgggaaatgc cgaagtgttc
     2641 ccacggttta tcgcggctga tagagttttc ggctaggtag tcggcgcggg attgcgacca
     2701 tgcccgaaaa acggttcttg tggaacagcc gaagcgtttt gccagttctt tagccgtaac
     2761 gtctcgcttg gtggttttca gttttggata ggccatattt caaagctccg tgatgatttt
     2821 tgttctgcct gcattgatgg ttgttttccg caaactattc cgatgagcct gcaattccga
     2881 aaaccgtcga ttgctggctg caatatcaaa cttgtaccaa acccagttag cgattgattt
     2941 ggagatacat ttcacttctt tttcccacat cgggacaggg aaatccccat ttacccgcat
     3001 acactggtaa tgcacttctt tcagccagcc ttgcaccgtg tagccctgct gtttgaacgc
     3061 caacacgttt ttgtgcgccc aacggctcac aaggttaaac accgtgcaat ttctgcttaa
     3121 tccgaccgct tccacgttag agcgaccgat atagggctta aacttgtcta aatccacgaa
     3181 atccgcaaga tactccaaat cgtagcccct gattgcgtca ggaacgccgc gcagcgtcag
     3241 ccaatgcgga tgttcgggat ttttcgtaat cagcgataca aagcccacat caccgcgcaa
     3301 tttcgcctta tatgctgctt caagtgcagc aagatagcgc agggcttttt gtctcccacc
     3361 gtattccgcc gtcagcacag gcgcggaaag cgcataggca aggtgtgcgc cgccgttttc
     3421 cctgttgatt gccgcccaag caggcatagg cagattattg tcttcccaag ccaaccccgc
     3481 cccttcgtaa tccaagtcaa agagcataaa cacacgcaga tgcggcggat tgacttggat
     3541 gtagcggcgt ttgatggcgg cagcgtaaga gcgcaccagc ataggcgctt ctttgaaatc
     3601 tttgcagtat ggcttgtgtg ggatacgttc ttgcaagaag aggtcgggtt gggtgtataa
     3661 ttggctcatg ttgtatctcg aaacccccgt gcagattggc gtttggcggg ggttttgctt
     3721 tgtctaagat ttgcagattg tatgcttgtt tttaagatga tacaactatg tcaaaataac
     3781 cataatcaga taacagcccg ataggggttc ttatttcaaa attttccaat ccgcaattta
     3841 gcgaagccag caggcgaagc ggtaaagctt ggagcgcagc agcgcgacct aagccggcca
     3901 gcagggcggc gttttggggg aaacatgaaa ccagttccga cagggcggcg tgcgtgttct
     3961 tccctgagtt cttcatggag tatcggcgaa atgccgtgat gaaatgccgt ttttttgagc
     4021 agaaagcagt caaaaacagg ggtattttgc ccttttgaca ggttcgagtg ccgccgaaaa
     4081 gcgaacaaag caactcatca tccgagtcag cccgaccgag tttgagactt tgacccgaca
     4141 gaagacccat ccgaatttag cccgctacat tcgggagcgg gttttggaag atggcaaagc
     4201 atccgac
//
LOCUS       DAWTCI010000112         4093 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWTCI010000112 DAWTCI010000000
VERSION     DAWTCI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.112"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            160..1241
                     /locus_tag="WM481_002016"
                     /pseudo
     CDS             160..1241
                     /locus_tag="WM481_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            1269..3698
                     /gene="hupB"
                     /locus_tag="WM481_002017"
     CDS             1269..3698
                     /gene="hupB"
                     /locus_tag="WM481_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFA9081249.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLTNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVFDPANPSPSRYLTYKATGVARSQPDPQEWVNKSTLFKLG
                     YNFNDRNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRTGVEYK
                     NELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWTA
                     DFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYDPKILASNQAKITMLIENRS
                     KYKFAYWNNVFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAHA
                     GFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKTNPELKAEKAKN
                     WELGLAGSGKAGSFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPV
                     WQNQNRTAAWVEGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALS
                     PWTAVYSLGYDAPSKRWGVNAYAARTAAKKPSDTVHSNDDLNKPWPYAKHSKAYTLFD
                     LSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSVREFGTVNRVNNKTHAGIQRFTSPG
                     RSYNFTIEAKF"
BASE COUNT         1094 a         1268 c          964 g          767 t
ORIGIN      
        1 ggtttttgtt aatccgctat aaaaaacaaa ggtagaaacg cggcaggcaa acgcaggcac
       61 gtccgatttc cgcgccgccc cataaaacag ttgacatcat ataacattta aattgataat
      121 caaaactatt attaatacat catcaagatt gagtatgcga tgaaatacaa agccctgccc
      181 ttactgccgc ttgccgccgc ccttgccgcc tgtgccgggg gggggtagcc gaaccgcacg
      241 tccccgtgtc catccccacc gccacgccgc tgcccgccgg cgaggtaacg ttatcaagcg
      301 ataacggcaa tatcgaaaac atcaacaccg ccggcgccgg aagcgcatcc gacgcgccga
      361 gccgcagcag acgctcgctc gatgccgccc cgcaaaacac atccggcatt tccattcggc
      421 agcgggaagt agaaaaagac tatttcggct ataaatcaaa agaaacatca ttcatcttca
      481 aaacacccgg cggcgcgcaa tacgcccttt catcctacgc cgaccccata accgtcagct
      541 actcctcccc cgatttcaaa attcccgacc gccacgcagg gcagcggctc gccgacggca
      601 gccgcatctt tatctgctgc agcgactccg gggctacttc gtatgcggaa attaccaaac
      661 aagattatat gaaattcggg gcgtggatag gtcccaacgg cgaaatcgac ctcttcgccg
      721 gcggcttccc cgtcggcaaa acgccgcctc ctgcgttctc ctacggcagc agcacgcccg
      781 aaaccgcatt gtcaaagggt aaaatcactt atcaggtttg gggcatccgc gtcagaaacg
      841 ggcaatttgt tacttcctct tatacgccgc cgaaaagcgg ttcttattat gggacacttg
      901 ccaatacccc cgtcctttcc tttatcaccg ccaatttcaa cagcaacaca ctggcaggca
      961 aaatcctcgg caacagcgat tacgggccgg atgtggatat tcaaaacgca acgattaccg
     1021 gtccgacctt ttccggcgat gccacgtccg gcgggaaaag cggcaaattg gaaggcaagt
     1081 tttttggcaa gttcgccagc acccgcagca gcgaagtcag cataggcggc aaaatcacct
     1141 ttgacggcga ccgctccctc gataccgtat tcggcggtgt gagttatgaa aaaaaacttg
     1201 atgacaccag tcaagatacc aaccacctta ctaaacaata aaccacctca tccctgcaaa
     1261 ggtttcttat gcccattccc ttcaaacccg tattggctgc cgccgccatc gcccaagcgt
     1321 ttcccgcctt tgcggcagac cccgcgccgc agtccgccca aacgctgaac gaaatcaccg
     1381 ttaccggcac gcacaaaacc caaaaactcg gcgaagaaaa aatccgccgc aaaaccttag
     1441 acaagctctt gaccaacgac gaacacgacc tggtgcgcta cgaccccggc atttccgtcg
     1501 tcgaaggcgg cagggcgggt tctaacggct ttaccatacg cggcgtggac aaagaccgcg
     1561 tcgccatcaa cgttgacggg ctggcgcagg cggaaagccg ctcttccgaa gccttccaag
     1621 aattgttcgg cgcgtacggc aacttcaacg ccaaccgcaa cacttccgag ccggaaaact
     1681 tttccgaagt aaccatcacc aaaggcgcgg actcgctcaa atccggcagc ggcgcattgg
     1741 gcggcgcagt caattaccaa accaaatccg caagcgacta tgtttccgaa gacaagccct
     1801 accatttggg gataaagggc ggcagcgtcg gcaaaaacag ccaaaaattc agcagcatta
     1861 ccgccgccgg caggctcttt ggtttggatg ccttattggt ttatacccgc cgcttcggca
     1921 aagaaaccaa aaaccgctcg accgagggcg atgtcgaaat taaaaacgac ggatatgtct
     1981 ttgacccggc caacccctcg cccagcagat accttaccta caaagccaca ggggttgctc
     2041 gctcccaacc cgacccgcaa gaatgggtaa acaaaagcac cctgttcaag ctgggctaca
     2101 atttcaacga ccgcaaccgc atcggctgga tttttgaaga ctcgcgcacc gaccgtttta
     2161 ccaacgagct gtctaatttg tggacgggta cgaccacgtc tgccgcaacg ggcgactacc
     2221 gccaccgcca agacgtgagc taccgccgcc gcaccggcgt cgaatacaaa aacgaattgg
     2281 aacacggccc gtgggacagc ctcaagctgc gctacgacaa gcagcgcatc gatatgaaca
     2341 cttggacttg ggacatcccg aaaaattacg atacaaacgg catcaacggc gaggtttacc
     2401 attcgttccg gcatatccgc caaaacaccg cgcaatggac tgccgatttt gaaaaacaac
     2461 tcgacttttc caaagccgtt tgggcggcgc aatacggctt aggcggcggc agaggggaca
     2521 atgccaactc ggattacagt tatttcgcaa aactgtacga ccccaaaatc ctcgcttcca
     2581 accaagccaa aatcacgatg ctgatcgaaa accggtcgaa atataaattt gcctattgga
     2641 acaatgtgtt tcacttgggc ggcaacgacc gcttccgcct gaatgcgggc atacgctacg
     2701 acaaaaacag cagcagcgcg aaagacgatc cgaaatacac caccgccatc cgggggcaga
     2761 ttccccattt gggttcggaa cgcgcgcacg cgggcttcag ctacggcaca gggttcgact
     2821 ggcggtttac caagcatctg cacttgttgg caaaatacag caccggcttc cgcgcaccga
     2881 cttcggacga aacctggctg ctgttcccac accccgattt ctacctgaaa accaatcccg
     2941 aactgaaagc ggaaaaagcc aaaaactggg aattgggcct ggcgggcagc ggcaaagctg
     3001 gcagcttcaa gctttcgggc ttcaaaacca aataccgcga ctttatcgaa ttgacgtata
     3061 tgggcgtttc gtcagacgat aaaaacaacc ccagatacgc cccgctttca gacggcacgg
     3121 cattggtcag ctcgcccgtt tggcaaaacc aaaaccgcac cgccgcctgg gtggaaggca
     3181 tagagtttaa cggcacttgg aacctcgaca gcatcgggct gcccaaaggg ctgcacaccg
     3241 gcctcaacgt cagctacatc aagggcaagg caacgcaaaa caacggcaaa gaaacgccca
     3301 tcaacgcgct ttcgccgtgg acggcggttt acagcctggg ctatgacgcg ccttccaaac
     3361 gctggggcgt caacgcctac gccgcgcgca ccgccgccaa aaagccgtcc gacaccgtcc
     3421 acagcaacga cgacttgaac aaaccgtggc cttatgccaa acacagcaag gcctatacgc
     3481 tgttcgacct ttccgcctac ctcaacatcg gcaaacaggt tacgctccgc gccgccgcgt
     3541 acaacattac caacaagcag tactacactt gggaatcttt acgcagcgtc cgcgagttcg
     3601 gcacggtcaa ccgcgtcaac aacaaaaccc acgccggcat ccaacgcttt acctcgccgg
     3661 gcaggagcta caatttcacc atcgaagcga agttctaagc tcctgatgtg ccaatgccgt
     3721 ctgaaacccg atgatttggg tttcagacgg cattttttac ggtcgcaccg ccgccccctt
     3781 tcaggcttgt accaagccca atcccgcaac tctccgtcat tcccagggaa gtgggaatct
     3841 agaaacgcaa agccacagga acttatccga aacaacagca acctaaaatt cgtcattccc
     3901 acgaaagtgg gaatccagtt cgttgagttt cagttattta aaataaattt tgaaacttta
     3961 atcccatcat tcccgcgaaa gcgggaatcc ggaacgtaaa atctaaagaa accgttttat
     4021 ccgataagtt tccgtaccga cggggctgga ttcccgcctg cgcgggaatg acggcggagc
     4081 ggtttctgtt ttt
//
LOCUS       DAWTCI010000113         3845 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWTCI010000113 DAWTCI010000000
VERSION     DAWTCI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.113"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..779)
                     /locus_tag="WM481_002018"
     CDS             complement(<1..779)
                     /locus_tag="WM481_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFA9081250.1"
                     /translation="MDTKEILGYAAGSIGCAILAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLAMSVLCLGLDQAYIREYNAAADKDILFKALFLPPLLSAAAIAAFLLSHPALPSE
                     ILFSLDDAAAGIGLVLFGLSLLPIRFLLLVLRMEGRALAFSSAQLVPKSALLLLLLTV
                     WLLHFPARTAVLTSVYALANLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIPL
                     ALDSLAYWGLTSADRLFLKKYAGLEQLGIYSMGISFGGAAL"
     gene            complement(974..2083)
                     /gene="ribD"
                     /locus_tag="WM481_002019"
     CDS             complement(974..2083)
                     /gene="ribD"
                     /locus_tag="WM481_002019"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFA9081251.1"
                     /translation="MFSDTNISMMENALRLAALGRFSTSPNPRVGCVIAHGRQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAKGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGLVLLKAAGIKTECGLLENKARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEEARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPPNSHLVTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGGGKDLFSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNGNAF"
     gene            complement(2115..2579)
                     /gene="nrdR"
                     /locus_tag="WM481_002020"
     CDS             complement(2115..2579)
                     /gene="nrdR"
                     /locus_tag="WM481_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HFA9081252.1"
                     /translation="MKCPFCSHPDTRVADSRLMEERNTVRRRRHCPGCGKRFGTLETV
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     ISSSELVEIVLEELFGQNTEAAVRFAALHKRFDSPEHFASWLAQAVKTGGKT"
     gene            complement(2608..3450)
                     /locus_tag="WM481_002021"
     CDS             complement(2608..3450)
                     /locus_tag="WM481_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFA9081253.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLVDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGISLFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     DDRVAVISTLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          794 a          988 c         1264 g          799 t
ORIGIN      
        1 aataatgccg ccccgccgaa cgaaataccc atcgaataaa tgccgagctg ttccaggccg
       61 gcatattttt tcaggaacaa acggtcggcg gatgtcagtc cccaataggc aaggctgtcc
      121 agtgcgagcg gtatgccgta gcgcagcccg cgatgcagga cggcgggcga aaacggtgcg
      181 cgccggacgg ccttcagacg gcatcggttt tgaaacagca aaaaggcggc ggcggcaagg
      241 tttgccagcg cgtaaacgga ggtcaggacg gcggtgcgcg ccggaaagtg cagcagccag
      301 accgtcagca gcaacagcag cagggcggac ttgggtacga gttgcgcgga cgaaaaggca
      361 agggcgcgcc cttccatacg caaaaccagt aagagaaagc ggatgggcag caagctcagt
      421 ccaaacagca ccagcccgat gccggcggcg gcatcgtcga gcgaaaacag gatttcagac
      481 ggcagggccg ggtgggaaag caggaaggcg gctatcgcgg cggcagacag cagcggcggc
      541 aggaacaggg ctttgaacaa aatgtctttg tcggcggcgg cattgtattc gcggatatat
      601 gcctgatcca gcccgaggca caacaccgac atcgccagtc ccgccgccgt ctgcatcaac
      661 acgatgcgcc cgatgtcgtc ggcggggaaa taccacgaca gcagcggcag gatgatgacg
      721 gctaaaatcg cgcagccaat cgatcctgcc gcgtagccga ggatttcttt tgtgtccatt
      781 tttgatgtcc ggtcggcggc gggatgctgc ctgtgccgtc tgaagccttt cttgatcgga
      841 atttgacggc tttcaggccg tcgcggctgc cggcggggtg cggcagcccg ggttgcgctt
      901 ttccgggcgg gcggcggtct gaacgggccg ttttttatcg gcgttattat atagtgaaac
      961 ggcggcaaac cctttaaaag gcgttgccgt ttttccggaa cacggttttg atgtcgtgtc
     1021 cgaggatttc ggtggaaacg ggtgtccata atggcggcgc ggaaagggcg gcgcggtttt
     1081 cgggcaggga aaacaggtct ttgccgccgc cgaggatttt gggcgaacgg tacagcacga
     1141 tttcgtccgc caaattttcc gccaaaaatg cggatgtgag ttcggagcct gcttcgacca
     1201 tgatttcgcc gaaaccttcg tcggcaagga ggcgcatcag atggtgcagg tcgattttgc
     1261 cgtctgccgt ttcagacggc atcaggatgc ggatgtgtgc gtgttcccga taggggcgga
     1321 gtttgtcttc atcgcgttcc aaggtggcga tgtaggtcgg agattgtccg tcggtaacca
     1381 aatggctgtt cgggggcagg cgcaggcggc tgtctaaaac gatgcgtgcg ggttggcgca
     1441 aagttggaaa agcgcggacg ttgagccggg gattgtctgc caacaccgtg ccgatgccgg
     1501 tcagcaccgc gcagctttcg gcacgcaaaa cctgtacgtc ggcacgcgct tcctcgccgg
     1561 taatccaaaa gctgctgccg tctgaaaggg cggttttgcc gtccagcgaa acggcgcatt
     1621 tgaggcggac aaaggggcgg cggcgttcga tgcgcgataa aaatccccgg ttgagttcgc
     1681 gtgctttgtt ttcaagtagt ccgcattccg tcttgatgcc cgctgctttg agcaggacaa
     1741 gccctttgcc tgcaaccggc gggttggggt cgcgcatggc ggcgacgacg cgtgttacgc
     1801 cggaacggag cagggcttcg gcgcagggcg gtgtgcgtcc gtaatggctg cacggttcga
     1861 gggtaacaaa ggcggtcgcg ccttttgcca tttcccccgc ctgacgcagg gcgtggactt
     1921 cggcatgggg ttcgcccgct ttgacgtgga agccctgtcc gacaatttgt ctgccgtgtg
     1981 cgataacgca gccgacgcgc gggttgggtg aagtggaaaa acgccccaaa gcggcaagtc
     2041 ggagggcgtt ttccatcatg gatatatttg tgtccgaaaa catagggata ccgtatcagt
     2101 atgggttggg ggaatcaggt tttgccgcct gttttgacgg cttgcgccag ccacgaggcg
     2161 aaatgttccg ggctgtcgaa gcgtttgtgc agggcggcga aacggacggc ggcttccgtg
     2221 ttttgcccga acagctcctc cagcacgatt tcgacaagtt cggatgagga tatgtcgcgc
     2281 tgacccgaag tgtagagcct gtgttccgtc aggcggacgg tttcgtcaat ctgttcgggt
     2341 gtcagggcgg attttcgggc ggcggcggtc aggtcgttgc ggaggcgttg tgcattgaag
     2401 ggcgaacgtt tcttgtccgg accgatgacg gcgggcattt tgagttcgac ggtttcgagc
     2461 gtgccgaagc gtttgccgca gccggggcag tggcggcggc ggcggacggt attgcgctct
     2521 tccatcaggc gggaatcggc aactcgggtg tctgggtggc tgcaaaacgg gcatttcatg
     2581 gtgtttcttc ctgatgccgt ctgaacgtca aaccgatacg ccggcggcgc gggcgatttc
     2641 caggccttct tcggcactca tatagacggg attttcggga cggtcgtgcc ggacgatgtt
     2701 gccttcgcgg aacatgacca gttcgttcac ggcaagttgg gaccacgatt catcgcgagt
     2761 cagcggcagg gtggagataa cggcgacgcg gtcgtccggc gtggttactt cggcaaaatc
     2821 gaccattaca tcgtcgtcga gcaggcgcgc tttgccgaac ggggcttggc ggacgatgta
     2881 gtgcagcagc gtgctggcgt gggcaaacag ggaaatgccg tctgaaagca tgaagttaaa
     2941 cagcccgaac ttgcggattt cgtgcgtcag ccccgcaatc gcgtcaaaca gcgtgtcgtc
     3001 gtcgggacgg gcggcaaagc gggtgcgcag gcggttgagg atgtggcaga acgcgcgttc
     3061 ggaatcggtt gtgccgacgg ggtggaaaaa ttctccttgt tcggggaaaa aatcgaccaa
     3121 atgcccgttg tgggcaaaca gccagtagcc gccccacatt tcgcgcataa agggatgggt
     3181 gttcgccagc gaggtttgac cttgcgaggc tttgcggatg tgggcgacga cgttttccga
     3241 tttaatctgg taggcgcgca cgaggtcggc gacgggggaa tttgcactcg gcttgtcgtc
     3301 atggaacagg cgcacgcctt tgccttcgaa aaagccgatg ccgaaaccgt cggcgtgatg
     3361 gtcggtaatg ccgcccctgc ggcggaagcc ttcaaaggaa aacataatat cggtcggcgt
     3421 attgcagttc atgcccagca gttgacacat agtttgtcca aatgattcag atggtcgcaa
     3481 gtattcggat tataccccga actgaaaatg ccgtctgaaa tacggcttgt tccccattat
     3541 tcccgcgaaa acagaaaaca aagacggaaa cttaagattc cgtcattccc gcgcaggcgg
     3601 gaatccgact tgtccggttt cggttgtttt tcgttccgta acttttgagc cgtcattccc
     3661 gcgcaggcgg gaatctagaa tttcaatgcc tcaagaattt atcggaaaaa accaaaaccc
     3721 ttccgccgtc attcccacga aagtgggaat ctagaaatga aaagcaacag gaatttatcg
     3781 gaaatgaccg aaactgaacg gactggattc ccgctttcgc gggaatgacg gcgacagggt
     3841 tgctg
//
LOCUS       DAWTCI010000114         3785 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWTCI010000114 DAWTCI010000000
VERSION     DAWTCI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.114"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            242..1024
                     /locus_tag="WM481_002022"
     CDS             242..1024
                     /locus_tag="WM481_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081254.1"
                     /translation="MKLRRLIMEMQLDKQTLTVVVRNSEPVELSVFAQSMMSLADDYE
                     SMCGSAGSHARLYIKEIRQGSIIAELAPLLPLAGTLFEGAGQILAYAKNFIEITDWLM
                     GKGKEPAGVTDSQIRNIANIMQPAASDKNGSIEINVNDNNGSVVNNITYNYFAANTVQ
                     NQARRILGERAEASESGDYGQMVMYFVQAAPTKETNQAVIEGIYSRPVKILIPEHIKR
                     EMFAEPYPFEKYYIVDVSVQTARGKPRLYKVTGYHGVVDGDD"
     gene            complement(1123..1524)
                     /locus_tag="WM481_002023"
     CDS             complement(1123..1524)
                     /locus_tag="WM481_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFA9081255.1"
                     /translation="MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAY
                     MHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVMIEIDPAKISRQQIRFNVSWPQY
                     LLDRVDEYTSANHETRSGFLAKAALLTMNQA"
     gene            complement(1626..1808)
                     /locus_tag="WM481_002024"
     CDS             complement(1626..1808)
                     /locus_tag="WM481_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFA9081256.1"
                     /translation="MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKD
                     LPTGTVKNIYKQAGLK"
     gene            complement(1978..2796)
                     /locus_tag="WM481_002025"
     CDS             complement(1978..2796)
                     /locus_tag="WM481_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HFA9081257.1"
                     /translation="MNQYAMRFAVIRFMPYVQTREFANIGIIITHPKSGYFDFKIEQR
                     YSRLSRFFRRFDSSVYKAATRAFAEELQRIGKLAAYSAPDQIRAMLDHLTRPREALIM
                     AAQPGVTLAPDRGQELNRLFDYFVARSFAKSQPEAELTRQIQAMLKPLQTAYPFKEST
                     IGDPSGFHASIPLVQKAENGEIRKIIKPIYFGQKDPADIYYKSDKWIAGIKRLRRGGY
                     IDRSEILFAYEPPERPDKAQEKALLDVLGDLEEQGIRLADNRSEGKIIRNFACG"
     gene            complement(2793..3491)
                     /locus_tag="WM481_002026"
     CDS             complement(2793..3491)
                     /locus_tag="WM481_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081258.1"
                     /translation="MDRAEQGVTSPFICMAENGLEYFVKGLHATRASQINEWIGGNMA
                     QALGLPVAPFELLEVGEELYEELPAKMKEIGKGICFGSLAQKGCALLEPADIPRIDTV
                     MQRQIAAFDWFVRNEDRTIGNPNLLYRNCNNSLIVIDHNCAFDTGFNPNNFLQNHIFS
                     SAFRQILEDWVLREEMELWLKSALPAYRKACDNLPPEWAWANEERDLPAAYNRSRTDE
                     TVRRIDNGTLWSIS"
BASE COUNT          901 a          872 c          981 g         1031 t
ORIGIN      
        1 cgtcctttgc cctcgcgcca aaccgattta ttctgccggt taattcgttc atcccgtttc
       61 cttcaagttg ttgtttgttt cgatgggtgt atagtactaa agtacttata tattgtaaag
      121 tacaatagta ctattatttt atatttagta tattagtatt tgattttcaa aagaatttat
      181 ttttgagatt ccgcaggcgc aaaaaaaccg cccgatattt cgggcggcgt attaaaatca
      241 gatgaaatta aggaggctta ttatggaaat gcaattggac aaacagacat tgacagtggt
      301 tgtcagaaac agcgagccgg tggaattgtc ggtatttgcg cagtctatga tgagccttgc
      361 cgatgattac gaatccatgt gcggcagcgc gggcagtcat gcgcggctgt atattaagga
      421 aatcaggcag ggcagcatta ttgccgagct tgcacccctc ttgcctttgg cgggtacgct
      481 ttttgaaggc gcggggcaga tattggcgta cgccaagaat tttattgaga ttaccgattg
      541 gctgatgggt aaaggcaaag aacccgcagg ggtaacggac agccaaatcc gcaatattgc
      601 aaatatcatg cagcctgccg catcggataa aaacgggtcg attgaaatca acgtgaacga
      661 caacaacggc tccgtcgtca ataatattac ctacaactat ttcgcggcaa acacggtaca
      721 aaatcaggca aggcggatac tgggcgaacg ggcggaagcg tccgaatcag gggattacgg
      781 gcagatggtg atgtatttcg tacaagccgc gccgacaaag gaaaccaatc aggcggttat
      841 tgaagggata tacagccgtc cggtcaaaat cctgattccc gaacacatca agcgcgaaat
      901 gtttgcagag ccttatccat ttgaaaaata ttatattgtt gacgtaagcg tacagacggc
      961 gcgcggcaag cccaggctct acaaagttac cgggtatcac ggggtagtcg atggggacga
     1021 ttgaaaaccg ccttttcggg cggctttgtc ggttttgtgt tgttttcagg ttcggcgggg
     1081 cgtgaaaaaa gcccgcgtgt gcgggcgggt tgaacgggct ggctatgcct gattcatggt
     1141 aagcagggcg gctttggcta aaaaaccgct acgggtttca tgatttgcgg atgtatattc
     1201 atccactcta tcaagaaggt attgcggcca gctgacgttg aatcggattt gctgccgact
     1261 gatttttgca gggtcgattt caatcattac ccatgttgcc ccatgataat caggttcttg
     1321 gctcaaatcg gcaatgctgc ttacggcaag gtttttgaac cctccgtcct caatcatacc
     1381 gtcaatatgc atataggcgg cagaacgggc gttggcgacg gcttcttcaa cggtgtcgcc
     1441 gcaagagaaa cagccgggaa ggtcggggat ggttacgcca tatgccgaat gttcgtcttt
     1501 gtgcagggcg gcagggataa acatttgatt tcctttaatt atgttgttca ggtttatggg
     1561 cgcgtgagat aacccgtcga ttgtttgatt agaagggggg cagcgggaaa ccccgctgcc
     1621 tgccgttact tcaaaccggc ttgcttatag atatttttta cagtacctgt cggcaagtct
     1681 tttttcgggt gcggcacggt tacacggcct ttttttgttg ggtgcttata ttgcgaatgg
     1741 ctcccggatt gtgcaacttt ataccaaccg tcttgtttga gtagggcaat aacgtctagg
     1801 ctattcaatt atcactccta atcttaatgc atggcacgcc tgccaatgtg cgtaataata
     1861 cacactaatt tttattttgt caatttttat gtgtatttgt gtgtaaaaat atctttcggc
     1921 agtagttgca gggaagggtg taaaaagccc gcataacgcg ggcggtcttg agggctgtca
     1981 gccgcaggca aagtttctga tgattttgcc ttcgcttcgg ttatcggcaa gccgtatgcc
     2041 ctgttcctcc aggtcgccca atacgtcaag caatgccttt tcttgtgcct tgtcgggacg
     2101 ctccggcggt tcgtaggcaa acaatatttc agagcggtcg atgtatccgc cgcgccgcaa
     2161 ccgttttatg ccggcaatcc acttatcaga cttgtaatag atgtcggcag ggtctttctg
     2221 tccgaaatag ataggcttga tgattttccg tatttcgccg ttttccgcct tttgcaccaa
     2281 ggggatggag gcgtgaaagc ctgacgggtc gccgattgtg ctttctttga aggggtatgc
     2341 cgtctgaagc ggctttaaca ttgcctgtat ttgtcgggta agttctgctt cgggttggct
     2401 tttagcaaag ctgcgggcga caaaataatc aaacaggcgg ttcagttcct gccccctgtc
     2461 gggggcgagg gttacgcccg gttgggcggc cataatcagg gcttcgcgcg ggcgggtcaa
     2521 atggtcgagc atggcgcgta tctgatcggg cgcggagtat gccgccaatt tcccaatccg
     2581 ctgtaattct tctgcaaagg cacgggtcgc cgctttatag acggacgaat cgaagcggcg
     2641 gaaaaaacgg ctcaatctgc tgtaacgctg ttcgatttta aagtcgaagt agccgctttt
     2701 gggatgggtt atgatgatgc cgatgttggc aaattcgcgc gtttggacat agggcataaa
     2761 ccgtatgacg gcaaaacgca tggcatattg gttcatgaga tgctccaaag tgttccgtta
     2821 tcaatgcggc ggacggtttc gtcggtgcgg ctgcggttat aggcggcggg caggtcgcgc
     2881 tcttcgtttg cccatgccca ctccggcggc agattatcac acgcttttct gtaggctggc
     2941 aatgcgcttt tcagccataa ttccatctct tcccgaagca cccaatcttc caaaatctgt
     3001 ctaaatgccg atgagaaaat atgattttgc agaaagttat ttggattgaa gccggtgtcg
     3061 aaggcgcagt tatgatcaat aacaatcaag gaattgttgc aatttctata caaaaggtta
     3121 gggttgccga ttgttctgtc ttcattacgg acaaaccaat cgaaagcggc gatttgccgc
     3181 tgcatgactg tatcgattcg cgggatgtcc gcaggctcca gtaacgcaca gcctttttgc
     3241 gccagtgagc cgaaacagat gcctttgccg atttctttca tctttgcggg caattcttcg
     3301 tacagctctt ctccgacttc caacagctca aacggagcaa cgggcaagcc caaagcctgc
     3361 gccatgtttc cgcctatcca ttcgtttatt tggcttgcac gggtggcgtg cagcccttta
     3421 acgaaatatt ccagaccatt ctccgccata cagatgaacg gcgaggtaac accttgctcc
     3481 gcacgatcca ttattgtctg tatctgcaac atttcttttc tttatcctat gtttcaacac
     3541 acaggacgac acataaagca ccgccctatg tgttgccctg attcggaagg ggttacgccc
     3601 ctcccaaaca gagtctgatc ctgccgccct aaagggcggg gtttcaaccg aaaaggaaat
     3661 acgatgaaat ttacactttg tgtaaatctg taaatccgac tttttgagat ttccccctag
     3721 aggaatttgt caatccagca cgctccacca aaatacccta ccaataacgg tcaggctgtc
     3781 taaag
//
LOCUS       DAWTCI010000115         3613 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWTCI010000115 DAWTCI010000000
VERSION     DAWTCI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.115"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..437
                     /locus_tag="WM481_002027"
     CDS             <1..437
                     /locus_tag="WM481_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFA9081259.1"
                     /translation="GAIYKVWQFVEDALRAVVPADSFEPTAQKLKLFKAGAATILFYE
                     DQNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGAGANLQHYNPLPDAAI
                     AKAWNIPENWLLRAQMVIGGIEGAAGEKVFEPVAERLKVFGA"
     gene            complement(492..947)
                     /locus_tag="WM481_002028"
     CDS             complement(492..947)
                     /locus_tag="WM481_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HFA9081260.1"
                     /translation="MNRRQFLGSAAAVSLASAASFARAHGHADYHHHHDMQPAAASAY
                     TAVRQTAAHCLDAGQVCLTHCLSLLTQGDTSMSDCAVAVRQMLALCGAVHDLAAQNSP
                     LTRDAAKVCLEACKQCAKACKEHSAHHAECKACYESCLDCIKECEKLAA"
     gene            complement(1203..2573)
                     /gene="ffh"
                     /locus_tag="WM481_002029"
     CDS             complement(1203..2573)
                     /gene="ffh"
                     /locus_tag="WM481_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFA9081261.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVSPPAI
                     ILMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAVVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLNGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKAGRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGIK
                     GMFPGL"
     gene            2795..3601
                     /locus_tag="WM481_002030"
     CDS             2795..3601
                     /locus_tag="WM481_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFA9081262.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDFPFMLHIGTSLLAYGLFGIATLLSVLSLLLHRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          790 a          895 c          972 g          956 t
ORIGIN      
        1 ggggcgcaat atataaggtg tggcaatttg tcgaagacgc gctgcgtgcc gtcgtgcctg
       61 ccgacagttt tgaaccgacc gcgcaaaaat tgaagctgtt taaggcgggc gcggcaacca
      121 ttttgtttta tgaagatcaa aatgtcgtca aaggtttgca ggagcagttc cctgcttatg
      181 ccgccaactt tcccgtttgg gcggaccagg cgaacgctat ggtacagtat gccgtctgga
      241 cgacacttgc cgcggtcggt gcaggtgcaa atctgcaaca ttacaacccc ttgcccgatg
      301 cggcgattgc taaagcgtgg aatattcccg aaaactggct gttgcgcgcg caaatggtta
      361 tcggtggtat tgaaggggcg gcaggtgaaa aggtctttga acccgttgcg gaacgtttga
      421 aagtgttcgg cgcataattt cgcggtcaaa aaaatgccgt ctgaaccccg ttcagacggc
      481 atttttcagt atcaggcggc gagtttttcg cattctttga tacagtcgag gcaggactcg
      541 taacaggctt tgcattccgc atggtgggcg gagtgttctt tacaggcttt ggcacactgt
      601 ttgcacgctt cgaggcacac ttttgccgcg tcgcgtgtca gaggggaatt ttgtgcggca
      661 aggtcgtgca ccgcgccgca taaggcaagc atctggcgca cggcaaccgc acagtcggac
      721 atagacgtgt cgccctgagt gagcagggac aggcagtggg tcaggcaaac ctgtccggca
      781 tccagacagt gtgcggcagt ttggcggacg gcggtgtagg cggatgcggc ggcaggctgc
      841 atatcgtgat gatggtggta gtcggcgtgt ccgtgcgcac gcgcgaaaga ggcggcggaa
      901 gccaaagaga cggcagcggc gctgcccaaa aattgacgac ggttcatgat tttttccttt
      961 atgtttcaac atgcaggtcc ggtataaggt acacacttac catactgccc tggccggatc
     1021 ggtacgattt tctgaataat tgcttgattt tgcaggtgtg aaattgtcgc acgagcaggc
     1081 atgttttagc tattcggaaa ttcgaggaag ggtgcatcaa cctgttttga aatgatgcgc
     1141 agacatggta acagacaaaa agccgtctga aaatcgtttt agacggcttt tgttttcaaa
     1201 cattaaagac cgggaaacat tccctttatt cccttcatgc ctttggccaa gcgcatcagt
     1261 ttgcccaaac cgttgccgct gaacatcttc atcatctgct gcatttgttc aaactgtttg
     1321 agcaatttgt tcacttcctg cacggtcgtg cccgcgccca tcgcaatacg gcgtttgcgg
     1381 ccggctttga gcagggcagg gttggcgcgt tctttagggg tcatcgagtt gatgatggct
     1441 tctactttgc ccatcgcttt ttcagccgta ccttcgggga tttgtttcga tatttgaccc
     1501 agttcgcccg gcattttcga catcaggttt tccaaaccgc ccatattgcg catttgctgg
     1561 atttgttctt taaaatcatt gaggtcaaag cctttgcctt tttgcagctt tttcgccatt
     1621 ttggcggcgg cttcttcatc aatacctttt tgaacgtctt caatcagggt caatacgtcg
     1681 cccatgccga ggatacggct ggcaagacgg tcggggtgga aaggttcgag gccgttgatt
     1741 ttctcgccga cgccgataaa tttaatcggt ttgccggtta catggcgtac ggacaatgcc
     1801 gcaccgccgc gcgagtcgcc gtccatcttg gtcaatacga cgccgttcag cggcagggct
     1861 tcattaaatg cctgagcggt atttaccgca tcctgaccca gcatggcatc gataacgaac
     1921 aaagtttcaa ccgggttgac caccgcatga agcgctttga tttcgttcat catctcttca
     1981 tcgattgcca aacggccggc ggtatcgacc atcaatacat cgtaaaaatg ttttttggcg
     2041 taatcaacgg cggcagttgc aatctcaacc ggtttttggt tggtatcgga tgggaaaaaa
     2101 tctacgccga cctgttcggc caacaggcgc agctgttcaa tcgcggcagg gcggtaaacg
     2161 tcggcggata ccaccaaaac ttttttcttc tgatcgtttt tcagcaggcg ggcgagtttg
     2221 ccgacggttg ttgtcttacc ggcaccctgc aaaccggcca tcaaaatgat ggcaggcggt
     2281 gaaaccgata aatccagtgt tttgttttcc ttgcccatca gttcgactaa ggcttggttg
     2341 accacgccga taaaggcttg gtcaggcgtc aggctgcccg ccacttcctg accgagggcc
     2401 ttttctttga cgttgttgac gaactctttg acgacaggca gggcgacatc cgcctcaagc
     2461 agggcgagtc ggacttcgcg caaggcttct ttaatattgt cttcggtcag tttggcctgc
     2521 ccccggatgt ttttgaagac attgctgaag cggccggtta aattgtctaa catacttggt
     2581 ccttggtctg aagtaagaat agcttgcccc atcaggggca ttctttgtta aaataaaatc
     2641 aaaataattt gatgcggctt gtgtgccgga cagcatatcg gcaaatccgt caaggcttgc
     2701 ccgaaacggg gattttacaa ttccaacgtt aaaagttcca atatttcata agcggccgca
     2761 tacggcgcaa cagtatagat agagaaagtc caccatgccg acagttttca tctttttgac
     2821 ggcggtttac gcaggattgg gtgcatttgc atggcactgc caacagcagg ggcgcggccg
     2881 ggattacccg tggaagacgg aattgccggt tttgggcgcg gcattgaccg tccacggcgc
     2941 ggcactgctg atgccggtca ttcaagacaa aatcatcatt atgggcttcg ggtattccgg
     3001 cagcctgatt gtttggatga tgctgtttat ttattttgcc ggcagctttt tttatccgct
     3061 gcgcggagtg cagttgctgc tgtatccttg cgccgcactg atgctgctgt caggtttggt
     3121 tttcccggga aaattctcgg gatatgaaat taccgacttt ccctttatgc tgcatatcgg
     3181 cacatcactg cttgcatacg ggctgttcgg catcgcaaca ttattgtccg ttttgagctt
     3241 gctgctgcac cggagcctgc accgcagaaa cttctccaag ctcgcaggat tcctgccgtc
     3301 gctgctcagt ttggaaaaac tcatgttcca ggccatgtgg gcaggtttca tcctgctgac
     3361 ctattccgtc gtcagcggca cattctttgc cgaagccgta ttcggcaaac ccatgacatt
     3421 tacccataaa accgtattcg gcatattgtc atggctgatt tacggaggac tgctgctcaa
     3481 acacagcatg accgcatggc gcggcaaaaa agccgccgtg tggaccatca tcggatttgt
     3541 cagccttatg attgcctata tgggcagcaa gttcgtattg gaaatcattc tgaaaagata
     3601 agaagagcca aca
//
LOCUS       DAWTCI010000116         3606 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWTCI010000116 DAWTCI010000000
VERSION     DAWTCI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.116"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(1..138)
                     /locus_tag="WM481_002031"
                     /pseudo
     CDS             complement(1..138)
                     /locus_tag="WM481_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(156..500)
                     /locus_tag="WM481_002032"
     CDS             complement(156..500)
                     /locus_tag="WM481_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081263.1"
                     /translation="MNLENYENILIKLLFHHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDPLPD
                     NMIINNFFEIND"
     gene            complement(899..1300)
                     /locus_tag="WM481_002033"
     CDS             complement(899..1300)
                     /locus_tag="WM481_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081264.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINFDKVEVIAVGYRNTGKTIKISIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDERYAAWISKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIES"
     gene            complement(1304..2053)
                     /locus_tag="WM481_002034"
                     /pseudo
     CDS             complement(1304..2053)
                     /locus_tag="WM481_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(2284..2643)
                     /locus_tag="WM481_002035"
     CDS             complement(2284..2643)
                     /locus_tag="WM481_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081265.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLDRI
                     TTNECINGIKKSIKKTN"
     gene            complement(2664..2870)
                     /locus_tag="WM481_002036"
     CDS             complement(2664..2870)
                     /locus_tag="WM481_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081266.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(2836..>3606)
                     /gene="mafB"
                     /locus_tag="WM481_002037"
     CDS             complement(2836..>3606)
                     /gene="mafB"
                     /locus_tag="WM481_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9081267.1"
                     /translation="VNPVTYAAAQQTLQGIHDLGNVSPAAQLAAASLLQDSAFAVKDS
                     INSAKQWADAHPNITATAQTALAVAEAAATVWRGKKVKLNPTKWDWVKNTGYKKPAAR
                     PMQTVDGEMAGKNKPPKPSTQQHSTHSDNNIGLPAPYVKPDTSISPTGTIQDRIRWTK
                     SKFPTEKSLNGHFKAHGKEFGDITIEDYQKMASDLLSKQTSDKILGYQTEHRRVRYDI
                     NNNIYVLANPKTFKIKTMFKPDLGKEYYDGEFKKDMGN"
BASE COUNT         1016 a          719 c          611 g         1260 t
ORIGIN      
        1 cgcataggtt accgggttta ccgcactgtc tgtaattgcc ccaactccca atccttgaaa
       61 acccgttaaa aaaggattaa ccgcaccttg gacgatgccg cgaatatcat ccatacggtt
      121 accccaccaa tttttattgg tttattggta tatcgttaat catttatttc aaaaaagtta
      181 tttattatca tattatcagg gagcgggtcg ttaaaaaaaa ctccctcaaa ttccattaaa
      241 ttagtattta tccagcacat aaacgaacca aagcgttctt tccctgagtg taaatattta
      301 aaattaaact caaaataagt ttttttgctt agaaaattta atggtttttc tatagtgaaa
      361 aaaaagccct tactcgtttt agttcgagat actattttaa ataagttttt attctcaata
      421 aaataagaat attcatttac taagttgtta tgatgaaaaa gtaattttat taaaatgttt
      481 tcataatttt ctagattcat ttctagctca atttaaaatg atttcattgt tgccgtacca
      541 atgcgaaatt taccatcaac aaaatttccc ttcactgata caggtacccc atcgcagaga
      601 tgtaggtcgg atatttgtat ccgacaaaaa tatttgacgc gtttatcgtt tccgaaatgc
      661 cgctgttaga aaatgtcgga ttcgagaatc caacctacgg ctatatccgt aaagtctgac
      721 cattgttcaa tgcggaaggc gcgaatattg cggacggaat gtagaaacca atatacttaa
      781 ttgccatgcc attctcatca aaatacctgt gctgtttaaa gtgcgtggtt cattacacga
      841 gtaattttat tttcaggctg cctgaaagaa agataggcaa cttattactc atcttacgct
      901 agctctcaat aattgaatac gttgcactag aaccaattat ttctatgaat gtttgctcaa
      961 acataaaaat atagtattta aagaattctc tattcaaagg aaaataactt tctttcgata
     1021 tccatgccgc ataacgctca tcaagaagtt cgaaaaaaga ttgaccacca ttttcatgaa
     1081 gcaaagctct atctgcatct tccgaaatta aatccataaa agtatctata aaatacgatt
     1141 catcaacaac cctaaaatga ataacatcct caatagaaat ttttatggtt ttacccgtat
     1201 ttctataacc aactgctata acttctactt tatcaaagtt tattgctgca ttatcaaaat
     1261 agaaatagcc agttgggaaa tctaagcttt ttaatttcat aatttatcgt gtatttccat
     1321 atctgatttt tatttttcgg ttattactaa ttgttatttc aagtgttggt tctccaccac
     1381 tgctatcatt acgaacatta accgcacgcc catcaggtaa attgcccgtt atcgttccat
     1441 taggacgtgt ttgtacattt cgaacattca gactattaaa atcatttagt gcgtcattgt
     1501 aatcccccac tttatcatac tgaacagcgg gaccttttgt tttactcctt tgagtagtat
     1561 tgctcaaaat gtctttgatt tgtaaatcac tgcgcacctt accggcttct atacgagctg
     1621 acacactact cgggctatag gggtcgctat caatcgcagg attaccaccg ctggtttttt
     1681 gtccgctagt ctgtttccca acctgctgta ctgtattgcc aatttcatct accgtaccta
     1741 acgccttagt ctgcatatgg cgggcagtag gttttttata accgttattt ttaacccaat
     1801 cccatttggc cgggttaagt tctacttttt taccgcccca aaccgtacct gcagcctctg
     1861 ctacggcaag ggcagtttgg gctgttgctg ttatattcgg atgggcatca gcccattgtc
     1921 tggcggaatt gatgccgtct tttaccgcaa aagcactgtc ctgtaatagg ctcgcggcag
     1981 caagttgtgc ttccggactt aaatttccta attcattaat atcttgtaaa gtttgctgcg
     2041 cagagatgta ggtcggatat ttgtatccga caaaaatatt tgacgcatct atcgtttccg
     2101 aaatgccgct gttggaaaat gtcggattcg agaatccgac ctacggctgc tgactatttt
     2161 cttgtttata taattctaca actgcaaact actatcaaat ctttttatct ctggctctac
     2221 actcagcaag aatattttta ttttacaaaa cataggctag ttatttgagt atattgtcaa
     2281 attctaattt gtttttttga tagatttttt tataccatta atacattcat tagttgtaat
     2341 tctgtcaagc gaatgtaatt tatcaatatc aaaacaatcg gtataaaccc tattcacagt
     2401 tttatcttgt atttccttat gaatacttaa tatattgaga tagtttagaa attttatttt
     2461 ctcagattga ggtaactcat caaaaccaag ccaataataa aaattgttat atgattttaa
     2521 aaaatcaaca atttttattt ttcctaaaac caaatcttcg actaactggg ttgcataaat
     2581 aatatcattt atgattttaa caggtaaatt atttgatggg taaccatggg gttccgtcat
     2641 cattggtttc tctaggtgct tatttatctg attggtaagc cttttgcgta agcttctttc
     2701 gcctccgcaa gtgtcatttc attaggacca ccatctatat tagtttctcc tgtattttgc
     2761 cactgacaaa cgtcacagat atcatagtcc ataacttcag ttccgcaaac aggacaatgt
     2821 agccatattt ctccgtcaat ttcccatgtc ttttttgaat tctccatcat aatactcctt
     2881 tcctaagtct ggtttaaaca ttgttttgat tttgaatgtt tttggattgg ccaaaacata
     2941 gatattgtta ttgatatcat agcgcactcg tctatgttcc gtctgataac ctaatatctt
     3001 gtccgatgtc tgttttgata acaaatcaga cgccattttt tggtagtctt caatggttat
     3061 atcgccaaat tcttttccat gagctttgaa atgtccattt aaagatttct cagtaggaaa
     3121 cttggatttt gtccatctga tgcggtcttg aattgttcct gtcggagaaa tagatgtatc
     3181 aggtttaaca tatggggcag gtaagccgat attgttatca gagtgtgtag agtgttgctg
     3241 cgtacttggt ttcggtggct tatttttccc ggccatttcc ccgtctacag tctgcatagg
     3301 tcgggcagca ggttttttat agccggtatt tttaacccaa tcccatttgg tcgggttaag
     3361 ttttactttt ttacctctcc aaaccgtagc tgcggcctct gctacggcaa gggcagtttg
     3421 ggcagtagct gtaatgttcg gatgggcatc agcccattgc ttggcggaat tgatactgtc
     3481 cttaaccgca aaagcactgt cttgtaatag gctcgcggcc gcaagttgtg ctgcgggact
     3541 tacatttcct aaatcgtgaa taccctgtaa agtttgctgt gctgccgcat aggttaccgg
     3601 gtttac
//
LOCUS       DAWTCI010000117         3551 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWTCI010000117 DAWTCI010000000
VERSION     DAWTCI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.117"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            421..633
                     /locus_tag="WM481_002038"
     CDS             421..633
                     /locus_tag="WM481_002038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HFA9081268.1"
                     /translation="MTNIKNMINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYG
                     RKEHGYNENYKNKLQKLYGVYAEGSA"
     gene            614..3297
                     /locus_tag="WM481_002039"
                     /pseudo
     CDS             614..3297
                     /locus_tag="WM481_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261022.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT          950 a          972 c          969 g          660 t
ORIGIN      
        1 gatataattc aaaatataaa aatgggttac atctggacat tacgaaattt ttattccttc
       61 gcctgaattc tattgtcaga ttcaaagaga cctcatcatg cgaaccacct caaccttccc
      121 tacaaaaact ttcaaaccgg ctgccatggc gttagctgtt gcaacaacac tttctgcctg
      181 cttaggcggc ggcggaggcg gcacttctgc tcccgacttt aatgcaggcg gcaccggtat
      241 cggcagcaac agcagggcaa cgatagcgga atcagcagca gtatcttacg ccggtataaa
      301 aaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg ttgcggttac
      361 agacagggat gccaaaatca aagcccccga atctgcatac cagagacttt tcaaacccaa
      421 atgaccaata ttaagaatat gatcaacctc aaacctgcaa ttgaagcagg ctatacagga
      481 cgcggggtag aggtaggtat cgtcgataca ggcgaatccg tcggcagcat atcctttccc
      541 gaactgtatg gcagaaaaga acacggctat aacgaaaatt acaaaaacaa attacaaaaa
      601 ctatacggcg tatatgcgga aggaagcgcc tgaagacaga ggcggtaaag acattgaagc
      661 ttctttggac aatgaggccg ttatagagac tgaagcaaag ccgacggata tccgccatgt
      721 aaaagaaatc ggacacatcg atgtggtctc ccatattatt ggcgggcgtt ccgtggacgg
      781 cagacctgca ggcggtattg cgcccgatgc gacgctacac ataatgaata cgcatgatgg
      841 aaccaagaac gagaaataat gtctgcagcc atccgcaatg catgggtcaa gctgggcgaa
      901 cgtggcgtgc gcatcgtcaa taacagtttt ggaacaacat cgagggcagg cactgccgac
      961 catttccaaa tagccaattc ggaggagcaa taccgccaaa cgttgctcga ctattccggc
     1021 ggtgataaaa cagatgaggg tatccgcctg atgcaacaga gcgattaggg caacctgtcc
     1081 taccacatcc gtaataaaaa catccttttc atcttttcga caggcaatga cacacaagct
     1141 cagcccaaca cactgaccct attgccattt tatgaaaaga cgctcaaaaa ggcattatca
     1201 cagtcgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1261 ctgccatgtg gcgcctgtcg gcatcctatg aagcaaccgt ccatttcacc cgtacaagac
     1321 cgattcaagt tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1381 tgcagagata cccgtggatg agcaacgaca acctgcgtac cacgctgctg acgacggctc
     1441 aggacatcgg tgcagtcgga gtggacagca agttcggctg ggggctgctg gatgcgggta
     1501 aggccatgaa cggacccgcg tcctttccgt tcggcgactt taccgccgat acgaaaggta
     1561 catccgatat tgcctactcc ttccgtaacg acatttcagg cacgggcggt ctgatcaaaa
     1621 aaggcggcag ccaactgcaa ctgcacggca acaacaccta tacgggcaaa accgttatcg
     1681 aaggcggttc tctggtgtta tcggatatgc acgtcaaaac caaaggtgca ctgatttata
     1741 acggggcggc atccggcggc agcctgaaca gccacggcat tgtctatctg gcagataccg
     1801 accaatccgg cgcaaacgaa accgtacaca tcaaaaggcg gtctgcagct gggcgacaaa
     1861 ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg acggtacggc gattaccgac
     1921 ggcaagctgt acatgtcggc acgcggcaag ggggcaggct atctcaacag taccggacga
     1981 cgtgttccct tcctgagtgc cgccaaaatc gggcaggatt attctttctt caaaaatatc
     2041 gaaaccgacg gcggtctgct ggcttccctc gacagcgtcg aaaaaacagc gggcagtgaa
     2101 ggcgacacgc cgtcctatta tgtccgtcgc ggcaatgcgg cacggactgc ttcggcagcg
     2161 gcacattccg cgcccgccgg tctgaaacac gccgtagaac agggcggcag caatctggaa
     2221 aacctgatgg tcgagctgga tgcctccgaa tcatccgcaa cacccgagac ggttgaaact
     2281 gcggtcgccg accgcacaga tatgccgggc atccgcctac ggcgcacaac tttccgcgca
     2341 gcggcagccg tacagcatgc gaataccgcc gacggcgtac gcatcttcaa cagtctcgcc
     2401 gctaccgtct atgccgacag tgccgccgcc catgccgata tgcagggacg ccgcctgaaa
     2461 gccgtatcgg acgggttgga ccacaacggt acgggtctgc gcgtcatcgc gcaaacccaa
     2521 caggacggtg gaacgtggga acagggcggt gtcgaaggca aaatgcgcgg cagtacccaa
     2581 actatcggca ttgccgcgaa aaccggcgaa aatacgacag cagccgccac actgggcata
     2641 ggacgcagca catggagcga aaacagtgca aatgcaaaaa ccgacagcat tagtctgttt
     2701 gcaggcatac ggcacgatgt gggcgatatc ggctatctca aaggcctgtt ctcctacgga
     2761 cgctacaaaa acagcatcag ccgcagcacc ggtgcggatg aatatgcgga aggcagcgtc
     2821 aacggcacgc tgatgcagct gggcgcactg ggtggtgtca acgttccgtt tgccgcaacg
     2881 ggagatttga cggttgaagg cggtctgcgc cacgacctgc tcaaacagga tgcattcgcc
     2941 gaaaaaggca gtgctttggg ctggagcggc aacagcctca ctgaaggcac actggtcgga
     3001 ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag ccgtcctgtc tgcgacggcg
     3061 ggcgtggaac gcgacctgaa cggacgcgac tacgcggtaa cgggcggctt taccggcgcg
     3121 gctgcagcaa ccggcaagac gggtgcacgc aatatgccgc acacccgccg ggttgccggt
     3181 ctgggggtgg atgtcgaatt cggcaacggc tggaacggct tggcacgtta cagctacacc
     3241 ggttccaaac agtacggcaa ccacagcgga caaatcggcg taggctaccg gttctgacgg
     3301 acagaaaaca gacagccgca aagatcacgg tctttgcggc tgtttcttat gaaaagaaaa
     3361 ccctattcca attgcctgct tctattgttt caagacttct tccaaagatt cggcatcaat
     3421 cagacgtata gcggattaac aaaaatcagg acaaggcggc gggccgcagg cagtacggat
     3481 ggtacggaac cgatccgccc ggtgcttcag caccttaggg aaccgttccc tttgagccgg
     3541 ggcggggcaa c
//
LOCUS       DAWTCI010000118         3512 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWTCI010000118 DAWTCI010000000
VERSION     DAWTCI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.118"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(299..514)
                     /locus_tag="WM481_002040"
     CDS             complement(299..514)
                     /locus_tag="WM481_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081269.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESVFL"
     gene            complement(545..3313)
                     /locus_tag="WM481_002041"
     CDS             complement(545..3313)
                     /locus_tag="WM481_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9081270.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSGENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSNKGNAMAAIGARKWLESGAS
                     AGVLYGHSRRGVAQNYRVGGGGQHIGNFGEEYLERRKQQYFVQEGGLKFNAGSGKWER
                     DLQRQYWKTKWYKKYEDPQELQKYIEEHDKSWRENLAPQYDITPIDPSGLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGAKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNAINYRFGGEYTGYYGSESEFKRAFGENSPAYKEHCDPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFAGYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVGST
                     GLAYTIRHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          808 a          867 c          871 g          966 t
ORIGIN      
        1 aaaataacaa tataaatcaa tttattacgg ttttattttt atttggtgtt gacgggcaac
       61 atatcatctg cgcggtaatg atgagatttt aggttgtggg atttatcgga aaaacagaaa
      121 cctctccgcc gccattctca cgaaagtggg aatccggaaa gctaaagcca caggaattta
      181 tcggaaatgg ctgaaattta tagtggatta acaaaaatca ggacaaggcg acgaagctgc
      241 agacagtaca aatagtacgg caaggcgagg caacgccgta cctgtttttg ttaatccact
      301 ataaaaaaac tgattcccgc ttttgcggga atgacgactc ataaattacc cgaaacaacc
      361 aaaaataacc gaaatcaaaa accggcttcc ctgcgtggga atgaaatgga tttttctttg
      421 agttgccgat gcgataaaac ggattggcat aggcttgcgg caggatgatg cgtttgggga
      481 aacaaatgcc gtctgaaggg ctttcagacg gcattgcagt ttttccacaa aacgcgggct
      541 gcctttaaaa cttgtagctc atcgtcatca agaaggtgcg tccgcgtgcg aaattggtca
      601 atacgctttt gcttgtgccg ccgtatttgc cgttgcacaa cgttttatca gcattacacg
      661 ttacgtcttc gtccttgtct ttcgggtcga acgagctgta ataacgctgc gttgccgcat
      721 cattgcccgc atcgagcgga tcgatataac gcctgtcgaa caggtttttg acttcggcgc
      781 ggaaaataag gtttttcttc ggctcgtaag cggcgtaaaa atcaaaaatc aaaggctgtc
      841 gggcaagggt ttcggtttgt ttgatggaac gcttgcccag ttgccggaca ttgctggtat
      901 ttcccccgtt ggtgccgtcg atatagcgtt cttcagccgt cgcgcggatg ctcttgccga
      961 aatagcgcat cgcgccgccc aaagtcagtt tgttgcccaa ccagcgcgta ccgacttcca
     1021 aacgtccgta atctcgcggc agggcggaaa ccctgctcag cccataacct tgtttgagtt
     1081 ggtcttcttt ggaggcattg ttgggcgatt cgctcgcatc gctgaaattg gtcggttgcg
     1141 tgcttttttg ataggcgtaa gaaaggttgg tgaaaaaacg cccataatcg taattcagct
     1201 ccagctcaaa accgtgtttg tgcactttgt ctttgaaatt gcggtgtcgg atggtgtagg
     1261 caagcccggt gctgccgacc cagctcggaa tatccccgtt caaatcccac catttcccgt
     1321 aaacgttgtg gatgtagttg tcaatgcggc tgcggtagcc gaccagtttc aatcctaata
     1381 tatcatcttg ttttaacaat ccttttttat aggtattgaa gccaaattgc caagtgtttg
     1441 cgcgctctgg ttttaaggcg gtgtgaacgc cggagtcgcc gatttgggaa aaatacattt
     1501 cttggatgtt gggcatacgg tgtgtgcgcg aatagccggc gaacggcatg aaataatcgc
     1561 cgaagtccgc actaatgctg accgaatggt tgttggcgcg ctttttgccg tattttttca
     1621 atacgggttc ataaagcccg cagctcgggt cgcaatgttc cttgtatgcc ggcgagtttt
     1681 ctccgaatgc ccgcttaaat tcgctttccg agccgtaata gcccgtatat tcgccgccga
     1741 aacggtagtt gattgcattg gtgctgtagt ttaagcggta aatgtctttt ttgagcgcgg
     1801 catcgaagta gaacgtgttg aaatattgac tgccggccgg ttggacgatg gttgattttt
     1861 ggggcagcag ccctttatcg cccttaaacc gccccaaata ggaataaagc ccgttgtcct
     1921 gatccggacc gtcgaaaaac agccccaatt cttcaggaaa gcggtttttg ccgtattcgt
     1981 tgtggaaata attgaagccc aaagtggttt gcaactcggt ttcgcggggc agccggaagg
     2041 tggcggtgtt gttgaggtcg aggattttcg cgttgttgta ggtttcaaaa tcttttaaaa
     2101 gcccccagcc tgtaaacttc gcccctttcg gatatttctg cctgcccgaa ttgtaggctg
     2161 cggtcagatt gaggttggta tacgggttca aagacaaacc gtaattgaat tgataattgc
     2221 ggttgatgat tttgcggctg ccgattttgg tgtttaaatc gcgaaattgc gccgtgtatt
     2281 tattgaatac gccgtcgtat tccaatttaa acagattgcc tgccgactgc tgcttcaggc
     2341 cggacggatc gatgggggtg atgtcgtatt gcggcgccag gttttcccgc cagcttttat
     2401 catgctcttc gatgtatttt tgcagttctt gggggtcttc gtatttttta taccactttg
     2461 ttttccagta ttgcctttgc aaatcccgtt cccattttcc gctgccggca ttgaatttca
     2521 aaccaccctc ttgtacaaaa tattgctgtt tgcgccgttc cagatattct tcaccaaaat
     2581 ttccgatgtg ctgcccgccg ccgcccacgc ggtaattttg cgccacgccg cgcctgctgt
     2641 gcccgtaaag cacaccggca gacgctccgc tttccagcca tttgcgcgca cctatcgccg
     2701 ccatcgcatt gcctttgttt gaattggtgc cggtcagacc ttttagcagc aggccgtagg
     2761 tattattgcc ctgaacgacg tcatccacgc ctaaagtccg cagattcgcc gaaccggcaa
     2821 ggctgttgat gcctgccgag ccgctgaagc tgcctttgac gacatccagt ccggcaataa
     2881 aattgctgtc gacagatgca ccgaattgag atgaaccgcc tgccctgccc gcatcggtag
     2941 aagtcgaata aaaggtctgc gtgatgccgt ccaccatcgt attgacccgc ccgaacccgc
     3001 tgtcgccgcg aatattcaaa gacacaatgc ccgagctttt atcttgctgt gtaaacgcac
     3061 cgggtatgct acgtacgatg ttgtcgaggt tttcgccgga tttgaatata tcctgacggg
     3121 tcgatacggc acgcgcatcg gtaaacactt ttttgtcttt cggtacgcgc ttcgccttga
     3181 cgtgcacgtc ttccaaaacc tgtatctgcg cctcgctgcc cgcgcgccct gcatcttctg
     3241 cataactatg atgatatagc ataacaccca taagataaaa acaaatcggc ttcaaccgga
     3301 aagaagatct catattttcc tcaacaataa acaatcaatc caacatgtct accgttttca
     3361 tcgaatccgc cctttcgacc acccggccct acgcaaccga accgtcattc ccacggaagt
     3421 gggaatctag gacgcggggt ttgggcaacc gttttatccg ataagtttcc gtgcggacag
     3481 gtccggattt ccgcctgcgc gggaatgacg gg
//
LOCUS       DAWTCI010000119         3507 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWTCI010000119 DAWTCI010000000
VERSION     DAWTCI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.119"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            13..1809
                     /locus_tag="WM481_002042"
     CDS             13..1809
                     /locus_tag="WM481_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFA9081271.1"
                     /translation="MQYKPLLLALMLVFSAPAVAAHDAAHNRSAEVKKQAKNKKEQPE
                     AAEGKKEKGKNAAVKDKKTGGKEAAKEFKKTAKNRKEAEKEATSRQSARKGREGDKES
                     KAEHKKAHGKPVSGSKEKNAKTQPENKQGKKGAKGQGNPRKGGKAEKDTVSANKKARS
                     DKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVSNSL
                     KQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEIAATKA
                     QISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKAL
                     AVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKISKDARKLLEQKG
                     NEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMS
                     NLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYS
                     TAPATVESIAPGTVSYADELDGYGKVVVIDHGENYISIYAGLSEISAGKGYTVAAGSK
                     IGTSGSLPDGEEGLYLQIRYQGQVLNPSGWIR"
     gene            1936..3411
                     /locus_tag="WM481_002043"
     CDS             1936..3411
                     /locus_tag="WM481_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFA9081272.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDNEVLPVQ
                     SIRTMAEVYGQIKANYYHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAEVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDGMVLKAVPEDYVYGMGGDPLAGIPAELKTIPMAVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERT
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAAKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKK"
BASE COUNT         1031 a          812 c         1025 g          639 t
ORIGIN      
        1 gaaagccgaa ccatgcaata caaacccctc ctgcttgccc tgatgcttgt cttttccgcc
       61 cccgccgttg ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacaggcg
      121 aagaacaaaa aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatgcc
      181 gcagtgaaag ataaaaaaac aggcggcaaa gaggcggcaa aagagttcaa aaaaaccgcc
      241 aaaaaccgca aagaagcaga gaaggaggcg acatccaggc agtctgcgcg caaaggacgc
      301 gaaggggata aggaatcgaa ggcggaacac aaaaaggcac atggcaagcc cgtgtccgga
      361 tccaaagaaa aaaacgcaaa aacacagcct gaaaacaaac aaggcaaaaa aggggcaaaa
      421 ggacagggca atccgcgcaa gggcggcaag gcggaaaaag acactgtttc tgcaaataaa
      481 aaagcccgtt ccgacaagaa cggcaaagca gtgaaacagg acaaaaaata cagggaagag
      541 aaaaatgcca aaaccgattc cgacgaattg aaagccgccg ttgccgctgc cactaatgat
      601 gtcgaaaaca aaaaagccct gctcaaacaa agcgaaggaa tgctgcttca tgtcagcaat
      661 tccctcaaac agcttcagga agagcgtatc cgccaagaac gtatccgcca agagcgtatc
      721 cgtcaggcgc gcggcaacct tgcttccgtc aaccgcaaac agcgcgaggc ttgggacaag
      781 ttccaaaaac tcaataccga gctgaaccgt ttgaaaacgg aaatcgccgc tacgaaagcg
      841 cagatttccc gtttcgtatc ggggaactat aaaaacagcc agccgaatgc ggttgccctg
      901 ttcctgaaaa acgccgaacc gggtcagaaa aaccgctttt tgcgttatac gcgttacgta
      961 aacgcctcca atcgggaagt tgtcaaggat ttggaaaaac agcagaaggc tttggcggta
     1021 caagagcaga aaatcaacaa tgagcttgcc cgtttgaaga aaattcaggc aaacgtgcaa
     1081 tccctgctga aaaaacaggg tgtaaccgat gcggcggaac agacggaaag ccgcagacag
     1141 aatgccaaaa tctccaaaga tgcccgaaaa ctgctggaac agaaagggaa cgagcagcag
     1201 ctgaacaagc tcttgagcaa tttggagaag aaaaaggccg aacaccgcat tcaggatgcg
     1261 gaagcaaaaa gaaaattggc tgaagccaga ctggcggcag ccgaaaaagc cagaaaagaa
     1321 gcggcgcagc agaaggctga agcgcgacgt gcggaaatgt ccaacctgac cgccgaagac
     1381 aggaacatcc aagcgccttc ggttatgggt atcggcagtg ccgacggttt cagccgcatg
     1441 cagggacgtt tgaaaaaacc ggttgacggt gtgccgaccg ggcttttcgg gcagaaccgg
     1501 agcggcggcg atgtttggaa aggcgtgttc tattccactg cgcctgcaac ggttgaaagc
     1561 attgcgccgg gaacggtaag ctatgcggac gagttggacg gctacggcaa agtggtcgtg
     1621 atcgatcacg gcgagaacta catcagcatc tatgccggtt tgagcgaaat ttccgccggc
     1681 aagggttata cggtcgcggc aggaagcaaa atcggcacga gcgggtcgct gccggacggg
     1741 gaagaggggc tttacctgca aatacgttat caaggtcagg tgttgaaccc ttcgggctgg
     1801 atacgttgat cggattgccg aaacggcagg ctttatgcaa atcgtgccga tatggtgcgc
     1861 tgttccgtga agtttgccgt ttgccgtacc tgtgttttcc ggtttttgga aatcaagaga
     1921 gaaagttaaa gaagaatgtc gaaacctgtt tttaagaaaa tcgcacttta tactttgggt
     1981 gcaatcagcg gcgtggccgt aagtctggcg gtgcagggtt ttgccgccga gaaggacggg
     2041 cgggataacg aagtcctgcc ggtgcaatcc atccgtacga tggcggaggt ttacggtcag
     2101 attaaggcaa actactatca tgacaaaccc gatgccgatt tgtttgaagg tgcgatgaag
     2161 ggtatggtgg ccggtttgga tccgcattcc gaatatatgg ataaaaaagg ttatgccgag
     2221 ataaaggagt ccaccagcgg cgaatttggc ggcttgggga tggaaatcgg gcaggaagac
     2281 ggttttgtca aagtggtttc gccgattgag gacacgcctg ccgaacgggc ggaggtgaaa
     2341 agcggcgatt tcattgtgaa aatcgataat gtttcgacgc gcggtatgac ggtcagcgaa
     2401 gcggtgaaaa aaatgcgggg caagccgggt acgaagatta ctttgacgct gtcgcgcaaa
     2461 aatgccgaca agccgatagt cgtcaacctg acccgtgcca ttattaaagt gaaaagcgtc
     2521 cgccatcacc tgatcgaacc cgattacggc tatatccgcg tgtcgcagtt ccaagagcgg
     2581 acggtcgaaa gcgtcaatac cgccgcaaaa gagctggtaa aggaaaataa aggaaaaccg
     2641 ctcaaggggc tggtgttgga tttgcgcgac gaccccggcg ggcttttgac cggcgcggtc
     2701 ggcgtgtcgg cggcgtttct gccgtctgaa gcggtcgtcg tcagcaccaa gggacgcgac
     2761 ggcaaagacg gcatggtact gaaagccgtt cccgaggatt atgtgtacgg tatgggcggc
     2821 gatcctttgg cgggtattcc tgccgagttg aaaacgattc cgatggcggt attggtcaat
     2881 tccggttcgg cttccgcgtc ggagattgtc gccggcgcat tgcaggacca caaacgcgcg
     2941 gtcatcgtcg gtacgcagag cttcggtaaa ggttcggttc agactttgat tcctttgtcc
     3001 aacggcagcg cggtcaagtt gacgaccgcc ctgtattaca cgccgaacga ccgttccatt
     3061 caggcacagg ggattgttcc cgatgtcgaa gtaaaagata aggaacgtac ttttgaaagc
     3121 cgcgaggcgg acctggtcgg acacatcggc aatcccttgg gcggcgagga tgtgaacagt
     3181 gaaacccttg ccgtaccgct tgaaaaagat gcggataagc ccgctgcaaa agaaaaaggt
     3241 aaaaagaaaa aggacgagga tttgtcttca aggcggattc cgaaccctgc gaaagacgat
     3301 cagttgcgta aggctttgga tttggtcaag tcgcccgagc agtggcagaa gtctttgggg
     3361 ctggcggcga aaaagccggt ttcaaataaa gataaaaaag ataagaagta ggttttgacg
     3421 atgccgtctg aaagggtttc agacggcata ttatgtttga aaggacaggt tatgagcgat
     3481 tttttaccgg aatttgaacc gtgcggc
//
LOCUS       DAWTCI010000120         3236 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWTCI010000120 DAWTCI010000000
VERSION     DAWTCI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.120"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..515
                     /locus_tag="WM481_002044"
     CDS             <1..515
                     /locus_tag="WM481_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFA9081273.1"
                     /translation="WGGKKVELNPAKWDWVKNTGYKKPAARHMQTVDGEMAGGNRPPK
                     SITSEGKANAATYPKLVNQLNEQNLNNIAAQDPRLSLAIHEGKKNFPIGTATYEEADR
                     LGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQANFETYTIDSNEKRN
                     KIKNGHLNIR"
     gene            520..879
                     /locus_tag="WM481_002045"
     CDS             520..879
                     /locus_tag="WM481_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFA9081274.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            956..1165
                     /locus_tag="WM481_002046"
     CDS             956..1165
                     /locus_tag="WM481_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081275.1"
                     /translation="MKLIDFEGNLVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKNKVLLLDKELKKIYDTWDKC"
     gene            1266..2114
                     /gene="mafB"
                     /locus_tag="WM481_002047"
                     /pseudo
     CDS             1266..2114
                     /gene="mafB"
                     /locus_tag="WM481_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            2111..2467
                     /locus_tag="WM481_002048"
     CDS             2111..2467
                     /locus_tag="WM481_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HFA9081276.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMVDWAYENIQSETVVEITENQI
                     IEYQNRGLWGLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            2641..3015
                     /locus_tag="WM481_002049"
     CDS             2641..3015
                     /locus_tag="WM481_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081277.1"
                     /translation="MIIQNEFNLYPSNMLPEGFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFAGNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
     gene            complement(3053..3236)
                     /locus_tag="WM481_002050"
                     /pseudo
     CDS             complement(3053..3236)
                     /locus_tag="WM481_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1158 a          486 c          650 g          942 t
ORIGIN      
        1 tttggggcgg taaaaaagta gaacttaacc cggccaaatg ggattgggtt aaaaataccg
       61 gctataaaaa acctgctgcc cgccatatgc agactgtaga tggggagatg gcagggggga
      121 atagaccgcc taaatctata acgtcggaag gaaaagctaa tgctgcaacc tatcctaagt
      181 tggttaatca gctaaatgag caaaacttaa ataacattgc ggctcaagat ccaagattga
      241 gtctagctat tcatgagggt aaaaaaaatt ttccaatagg aactgcaact tatgaagagg
      301 cagatagact aggtaaaatt tgggttggtg agggtgcaag acaaactagt ggaggcggat
      361 ggttaagtag agatggcact cgacaatatc ggccaccaac agaaaaaaaa tcacaatttg
      421 caactacagg tattcaagca aattttgaaa cttatactat tgattcaaat gaaaaaagaa
      481 ataaaattaa aaatggacat ttaaatatta ggtaattata tgagattaaa attaaaaaat
      541 tatgattgtt ctgactttga tttggaagat ttcccacaag acaagttaga aaatttttgt
      601 atacttttga cattatcaat aggttttgat gaaagtaatg gtgctgatta tttttatgtt
      661 tatatatata gcaccgaatg gttactttca aatatacata gaccgatgtc attaaaaaac
      721 tcaattgtta caaaccgttt taatattgaa cacatattaa aattgataaa tgatatattg
      781 gaaatatgta attcaacgtc tgaagataag tctatatcaa atttggcaaa atattttgac
      841 tgggaatttg acgattataa tctaaatatc caagactaaa gatggtggga cagcatttag
      901 accaaaaaca ggtaaacagt attttgatga caaattaaaa taaaaggaat tattcatgaa
      961 attaattgat tttgaaggga atttagttaa aattagtctc gataaagatg agttgtatat
     1021 tattcaagca attgtaggag aaatttattc aggcgtatgc gtggattgta gagattttga
     1081 gataattcat ggtgttgaaa aaaataaagt tcttcttttg gataaagaac ttaaaaaaat
     1141 atatgatact tgggataaat gttagctaga tagggtgggc actcgttgcc caccttttgc
     1201 cgtatattaa aaatcctttt caggctgcct gaaactgggt gggcatcaat gccaatccta
     1261 actgtgcgga tgaagcagga aaactgatat gggaagacga ccccgataaa aattggcggg
     1321 ctaaccgtat ggatgatatt cgcggcatcg tccaaggtgc ggttaatcct tttttaacgg
     1381 gttttcaagg agtagggatt ggggcaatta cagacagtgc ggtaagcccg gtcacagata
     1441 cagccgctca gcagactcta caaggtatta atgatttagg aaaattaagt ccggaagcac
     1501 aacttgctgt cgcgagccta ttacaggaca gtgcctttgc ggtaaaagac ggcattaatt
     1561 ccgccagaca atgggctgat gcccatccga atataacagc aacagcccaa actgcccttg
     1621 ccgtagcaga ggccgcaggt acggtttggc gcggtaaaaa agtagaactt aacccgacca
     1681 aatgggattg ggttaaaaat accggctatg aaaaacctgc tgcccgacct atgcagactg
     1741 ttgacgggga aatggcaggt gggaataaac ctattaaatc tttaccaaac agcgccgctg
     1801 aaaaaagaaa acaaagtttt aagaagttta gtagcaactg gagttcagca agttttgatt
     1861 cagtgcacaa aacactaact cccaatgcac ctggtatttt aagtcctgat aaagttaaaa
     1921 ctcgatacac tagtttagat ggaaaaatta caattataaa agataacgaa aacaactatt
     1981 ttagaatcca tgataattca cgaaaacagt atcttgattc aaatggtaat gttgtgaaaa
     2041 ccggtaattt acaaggtaag caagcaaaag attatttaca acaacaaact catatcagga
     2101 acttagacaa atgaatgaac acaacctgtt aattttctgt ttaaaagaca atgtttcaat
     2161 tagtgaatat actgaaatgg ttgattgggc ttatgaaaac attcaatctg aaacagttgt
     2221 agaaattacg gaaaatcaaa ttattgaata tcaaaatcgt ggattatggg ggcttgtttc
     2281 tgaaattacc gataattggt tatttggacc aagtgagggg gattggctaa tagataagga
     2341 aagtattttg gctgtaaaag aaaaattaca aaattcagat ttttctacag agcccttagt
     2401 gaaaaatatt attcatgtac ttgaatatgc tataaaaaat gaaaaaacag taatttttca
     2461 tttttgaaac taatctaatt tttagtaata ttgatgcaga gcaagcagca ttagatgccg
     2521 caaacatggg gagaagctat tcaatttaga attaaaaaaa caaagtgaaa atggactagc
     2581 accaccaaat tggtctaccc agtttcctaa tggtagtatt tatgatacta aggtaacgaa
     2641 atgattattc aaaatgaatt taatttatat cctagtaata tgcttcctga agggttttgt
     2701 tatcctgaaa agtatgttcg tatctctaac gatacatctt taatacctta tattcagcca
     2761 cataattttc actggtggtt tgagaattat ggaacagaag gggcagaagt agcttatata
     2821 tttagaaatt ccatcctgcc tgatttaaat cttatcccat tcgctggtaa tggagaatgg
     2881 gaagcttatt ttgatggtaa tgatgtaaca ggaaatccta gggttattgt tattaattta
     2941 gataatatag aaaaccatga attttttaat agttttgaag agtggcttga attagcaatt
     3001 aaggatactt ggtaagcagc tatctataaa gagatgaggc cgtcctggac aactaggata
     3061 aactcgattt tactaattgt tttaaaatgg aacaagaact tttatctcac tgttgttaaa
     3121 acgccgttcg cactccttta aatacggctc aaaatgctct ttgggaatgc cgtcaaactt
     3181 gcgtaaatga cgttttgccc ggttccaaaa gttcccaatt ccattaatat ggtttt
//
LOCUS       DAWTCI010000121         3076 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWTCI010000121 DAWTCI010000000
VERSION     DAWTCI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.121"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..1554)
                     /locus_tag="WM481_002051"
     CDS             complement(<1..1554)
                     /locus_tag="WM481_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFA9081278.1"
                     /translation="MPPVHEVIMKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQ
                     RKGNIYKGIITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFLGYEGGRARIQDVLKE
                     GMEVIVQVEKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAA
                     MAQLDIPNGMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESS
                     LLIRAIRDYFRPDIGEILVDNQEVYDQVAEFMSYVMPGNAGRLKLYEDHTPLFSRFQI
                     EHQIESAFSRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEE
                     VARQMRLRDLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLEL
                     SRQRLKPALGESSHAACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPV
                     DVATFLLNEKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKR
                     VAEPEEDESA"
     gene            2023..3015
                     /locus_tag="WM481_002052"
     CDS             2023..3015
                     /locus_tag="WM481_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFA9081279.1"
                     /translation="MKTHEISKGSVSLIGVAEHEAGQRLDNYLIKILKGVPKGYIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFEVVYEDDA
                     LLVVNKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGKPLVLKAEPPPDLAQFAVMLENGTKM"
BASE COUNT          595 a          791 c          971 g          719 t
ORIGIN      
        1 ggcggattcg tcttcttcag gctcggcaac gcgcttgtag ctcggttcgc cgtcttcttc
       61 tacgtcgtcg gtgcggatgc ggttgatttc gtagtgcgga ttttcgaggt ggatgttcgg
      121 aatcaggacg acgttcacat ccaaacgctc ttccattgca aacagctcgg cgcgtttttc
      181 gttcagcagg aaggtggcga catcgacggg cacttgggcg cgcacttcgc cggtgttgtc
      241 cttcatcgct tcttcctgaa ccatgcgcaa aacgtgcagg gcggtggatt cgatgccgcg
      301 gatcacgccg gtgccggcgc agcgcggaca ggcggcgtgg ctgctttcgc ccaaagcggg
      361 tttcaggcgt tggcggctca attccaaaag tccgaaacgg gagagtttgc ccatctgtac
      421 gcgggcgcgg tcttttttga gtgcgtcgcg caggacgttt tccacatcgc gctggtgttt
      481 ggggttttcc atgtcgatga agtcgatgac gaccaagccg cccaagtcgc gcaggcgcat
      541 ttgtcgggcg acttcttcgg cagcttccat attggttttg aaggcggtgt cttcgatgtc
      601 cgcgccgcgt gtggcgcgtg cggagttcac gtcgatggag acgagggctt cggtgtggtc
      661 gatgacgatc gcgccgccgg agggcaggct gacgctgcgc gagaacgcgc tttcgatctg
      721 gtgttcgatt tggaagcggg aaaacagcgg cgtgtggtct tcgtagagtt tcagacgacc
      781 tgcgttgccc ggcatgacgt agctcatgaa ctcggcaact tggtcgtaaa cttcttgatt
      841 gtccaccaaa atttcgccga tgtcgggacg gaagtagtcg cggatggcgc ggatgagcaa
      901 cgagctttcc ataaagagca ggtaggggtc gtgatgcgct tttcctgctt cttcgattgc
      961 ctgccagagt tgtttgaggt agttcaaatc ccattccaac tcttccgcgc tgcggccgat
     1021 gccggcggta cgcgcgatga tgctcatgcc gttcggaatg tcgagttgcg ccatcgcggc
     1081 tttgagttct tgacgctctt cgccttcgat acggcgggat acgccgccgc cgcgcggatt
     1141 gttcggcatc aataccagat agcgtccggc gaggctgatg aaggtggtca gcgccgcgcc
     1201 tttgttgccg cgctcgtctt tttcgacttg gacgatgact tccatgcctt ctttgagcac
     1261 gtcctgaatg cgcgcgcgcc cgccttcgta gccgaggaaa tacgaacgcg atacttcttt
     1321 aaacggcaaa aagccgtggc ggtcggttcc gtaatcgacg aaacacgctt ccagcgacgg
     1381 ctcgatgcgg gtaatgatgc ctttgtagat attgcctttg cgctgttctt tgcccagcgt
     1441 ttcgatgtcc aaatccagca ggttttgtcc gtcgacgatg gcaacgcgca gctcttcggc
     1501 ctgcgttgcg ttgaataaca ttcttttcat gatcacctcg tggacaggcg gcattcgggc
     1561 gcgccggtcc ggttcggcat tccgtaaggc tgggtttccg atgtcttcgg ataaaaccgg
     1621 taatcagttt ttgagttgaa aatccgcagg gatgcacgtt ccggggaacc gtgtgcggaa
     1681 gggtcggata aagaaggcta taaagatcga tgcggcggtc tgcctgccgc gttccgaacg
     1741 ctgcggtcgg aaaaatgggc gccggcttct tcttgttatc gtgatgcctg tgttttcggg
     1801 cggtttgcgt tcggggcttg gggctccgct gccgtcttac ttccgcgccg aaacggcaaa
     1861 atcaattcaa acttgactac gttctgcgcc tgccggctgg gaacaggcgc agggaaaatg
     1921 ctttgcggag tgcgttttta atataaaatt ccgttttaaa gtaaaccgtt tcagggggcg
     1981 cggcggacgc gctttctgct gaaacggatg ttcggattat agatgaaaac gcacgaaata
     2041 agcaaaggtt cggtcagcct gataggggtt gccgaacatg aggcgggtca acgccttgat
     2101 aactatctga taaaaatcct caagggcgtg cccaagggct atatccaccg cattatccgc
     2161 gccggcgagg tgcggttgaa caagaaacgc tgcaaacccg acagccgtat tgcggaggga
     2221 gatacggtgc ggattccgcc tgtgcgcgtg gcggagaagg aaatgccgtc tgaaaggcgt
     2281 gccgccgtac cggcgcgtgc gtttgaggtg gtgtatgagg acgacgcgct actggtggtc
     2341 aataagcctt ccggtgtggc ggtacacggt ggcagcggcg tgagtttcgg cgttatcgaa
     2401 cagttgcgcc gcgcccgtcc ggaggcgaag tatttggagt tggtccaccg tttggacaag
     2461 gatacgagcg gcttgttgat ggtggcgaag aaacgcagcg cgctcgtcaa actgcacgaa
     2521 gctatccgta acgaccaccc caaaaaaatc taccttgcgc tgggggtggg caaactgccg
     2581 gacgacaatt tccatgtcaa actgcccctg ttcaaatata ccggcgcaca aggcgaaaag
     2641 atggtacgcg tcagcgagga cgggcagtcg gcgcatacgg tgttccgtgt gttaagccgt
     2701 ttctcagacg gcattttgca cggtgtcggg ctgtcgcacc tgactttggt gcgggcaacg
     2761 ctgaaaacgg gccgtacgca ccaaatccgc gtgcatctgc aatctcaagg ctgtccgatt
     2821 gcgggcgacg aacgctacgg cgattatcag gcgaaccggc gtttgcagaa attgggtttg
     2881 aagcgcatgt ttttgcacgc gtccgagctg cacctgaacc atccgctcac gggtaagccg
     2941 ctggtgttga aggcggagcc gccgccggat ttggcgcagt ttgcggtgat gttggaaaac
     3001 gggacgaaaa tgtgaacccc gatgccgtct gaaggcttca gacggcatcg gggcgtgaaa
     3061 gtatagtgga ttaaca
//
LOCUS       DAWTCI010000122         2775 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWTCI010000122 DAWTCI010000000
VERSION     DAWTCI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.122"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            444..662
                     /locus_tag="WM481_002053"
     CDS             444..662
                     /locus_tag="WM481_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081280.1"
                     /translation="MANSNSGHSKKLRAATAAAATKAKLASGEYRQFSVQGRAEDVEL
                     ILAAVEKAGGSRVQALAKICRRYLEGLS"
     gene            complement(679..1038)
                     /locus_tag="WM481_002054"
     CDS             complement(679..1038)
                     /locus_tag="WM481_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081281.1"
                     /translation="MQVLLGEDFKRALKNYPKEDRRKIAEFIAHVQQNGLSGLPGRNK
                     SSDNVPADDPQWLEKVRFAQRHNLWHYHIGIPKYNGGRYGDLTSAYILHYTLCDGFIK
                     IIGFDRHPPFILPDIPK"
     gene            complement(1039..1578)
                     /locus_tag="WM481_002055"
     CDS             complement(1039..1578)
                     /locus_tag="WM481_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HFA9081282.1"
                     /translation="MLNAYDVADFFLSPFEEEDGEQISNLKLQKLLYYAQGYALAILN
                     RPLFAENIEHWQHGPVVPCIYRTYKKYGGSPLPAAHIEPDKYADEELVVLNRVRKEQG
                     CYTAWALRNKTHQEAPWIQTRQGEVIGIALMGEYFRHALPQTDYNFNLEKLKTAVEDS
                     FVSVPHFNGADDLEKWLEQ"
     gene            complement(1738..2442)
                     /locus_tag="WM481_002056"
     CDS             complement(1738..2442)
                     /locus_tag="WM481_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9081283.1"
                     /translation="MTNRDLKAIRIDNLKRFFENKKLPVKDKSLLSQLMSGKASFGEK
                     VSRRLEEEYGMGSLYLDSVPSEPTPVLINPDLPHEVKDIHRPMMWSSNDPLPDDDYVF
                     VPYLKESCFKGGAGAYEIPDYNGYRLPFGKSTLRRKGINPDNVFCCTLTGDSMEEKIA
                     EDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYPDETAPL
                     DSLSVIGRVFWWSVLD"
BASE COUNT          674 a          603 c          712 g          786 t
ORIGIN      
        1 tttagtaaac acttaacatc tagtcaagta aaatgtttag tgattattta ataaatactg
       61 aacattttga ttattaaaag aatttatttt tgagatttcg caggcgcaaa aaaaaccgcc
      121 tattaaggcg gttgtttagg aaagaatgcc ctctgcgcgt gtagggtatt tcgatacttg
      181 cgtttattat atcatatgat ataataaact catagattag gaaatgaaga aaccccgtat
      241 cagccgatac ggggcaacct gaaagagaaa ggaggcaatg tgattaagaa aatcctaatc
      301 tgtgtgattt tgtatttcct tgcaagctcc ccagcgtggt agggaaatac aaaacaagtg
      361 attaacgatt aaccatttgc agggcagtta aggaggctgc ccttcctctt atctttccca
      421 tactctacta taaggattta aaaatggcaa attccaacag cgggcattct aaaaaattgc
      481 gcgcggcaac ggcggcggcg gcgacaaagg caaaactggc aagcggcgaa taccggcagt
      541 tttcagtgca ggggcgtgcc gaagacgtgg agcttatcct tgccgccgtg gaaaaagccg
      601 ggggcagccg cgtacaggct ttggcaaaga tttgcaggcg gtatctcgag gggctgtctt
      661 aaggggggta agacaaggtt atttgggaat atcgggcagg ataaaaggcg ggtgtctgtc
      721 aaaaccgatg attttgataa agccgtcgca aagggtatag tgaaggatgt aggcggatgt
      781 caaatcgcca tacctgccgc cgttgtattt gggaatgccg atgtgatagt gccaaaggtt
      841 gtgccgttgg gcaaatcgga ctttttccag ccattgagga tcgtctgccg gtacgttgtc
      901 gctggatttg ttcctgccgg gtaaccctga taagccgttc tgttggacgt gtgcgataaa
      961 ttccgcaatt tttctgcggt cttctttggg gtagtttttc aacgctcttt tgaagtcttc
     1021 gccaagcagc acctgcattt actgctccaa ccatttttcc aagtcgtcgg cgccgttgaa
     1081 atgcgggacg ctgacaaagc tgtcttcaac ggctgttttg agtttttcaa gattgaaatt
     1141 gtaatccgtc tgcggcagcg catggcggaa atattccccc atcagcgcaa tccctatgac
     1201 ttcgccctgc cgggtctgta tccacggcgc ttcctgatgg gttttattgc gtaatgccca
     1261 agcggtgtag cagccctgct ctttacggac acggttgagg acaaccaact cttcgtccgc
     1321 atatttgtcc ggttcgatat gggccgcagg caatgggctg ccgccgtatt ttttgtaggt
     1381 gcggtaaatg caggggacta ccggaccgtg ctgccagtgt tcgatatttt cggcaaacag
     1441 ggggcggtta agtatggcaa gggcgtagcc ttgtgcgtaa tacaggagtt tttgaagttt
     1501 gagattggag atttgctccc cgtcctcttc ttcaaaaggg gaaaggaaga aatctgccac
     1561 gtcgtacgcg ttcagcattt tctgtttccg ttgttatttt tctgtatatt aaggccgccg
     1621 gcccattatt tcaagaaata atttgtgcaa attatccggt atcggcaggc tgtccgcgcc
     1681 gtcggcatat cgggcggata tatttgaaaa atacttgccc tgccgtttca gacggcatca
     1741 atccaacacg ctccaccaaa agaccctgcc gataacggac aggctgtcca aaggggcggt
     1801 ttcgtcggga tagaagccgc tgttgtggct gcgtatcagc acgctgttgc cgggctgccg
     1861 tatcaggtat ttgacgcgga acatgccgtc ttgggcgaag gcgtatattt tgccgtcccg
     1921 tatggcggtt tcgcccgtat ctacggcgat tgccgcgtct tctgcgattt tttcctccat
     1981 gctgtcgccg gtcagggtgc agcagaagac gttgtcgggg ttgatgcctt tgcgccttaa
     2041 cgttgatttg ccgaacggca ggcggtagcc gttgtagtcg gggatttcat acgcgcccgc
     2101 cccgcctttg aagcagctct ctttgaggta ggggacgaaa acataatcat cgtcgggcag
     2161 cgggtcgttg ctgctccaca tcatcgggcg gtggatgtct ttgacttcgt ggggcaggtc
     2221 ggggttgatg aggacgggcg ttggttcgct cggaacggaa tccaagtaca gactgcccat
     2281 cccatattct tcttcaagcc gtctgcttac cttttcgcca aaagatgctt taccgctcat
     2341 caattgagat aaaaggcttt tgtctttaac cggcaacttt ttattttcaa agaatctttt
     2401 taaattatcg atacggatgg ccttcagatc tctattagtc attttcgcgc tccattcaga
     2461 ttgggattca attttattta gtttctgcta aacaaacaaa tattaaattt tcttgcctat
     2521 gcgtttaata tttgctaaac tcattaaata tttacggtga aactacagaa tgactcattc
     2581 agaatttatt aaaacgctgt cagaacagcg tggtgcaaaa acggaatacg ctaaaaaact
     2641 tggcttatcc ctgtcttttc tatggcagat agaaaacgga cgggcagtaa cgccaaagcg
     2701 tctttataaa gacgttatgt tgttgacaaa aaataaggtt tctatttctg aattaatttc
     2761 ggagttcagg gtttg
//
LOCUS       DAWTCI010000123         2705 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWTCI010000123 DAWTCI010000000
VERSION     DAWTCI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.123"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            300..1097
                     /gene="truA"
                     /locus_tag="WM481_002057"
     CDS             300..1097
                     /gene="truA"
                     /locus_tag="WM481_002057"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFA9081284.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQAALETALARI
                     AGESVATTVAGRTDTGVHAAAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQTA
                     PGFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAALLVGEQDFS
                     SFRAAGCQAKSPVKTIYRAGLTQSAGLIRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPGAYGIVRPQIPEWL"
     gene            1614..2660
                     /locus_tag="WM481_002058"
     CDS             1614..2660
                     /locus_tag="WM481_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFA9081285.1"
                     /translation="MKKSLIALTLAALPVAATADVTLYGAIKAGVQTYRSVGHQEGKV
                     VGVGTGSEISDFGSKIGFKGQEDLGNGLKAVWQLEQGASVAGTNTGWGNKQSFVGLKG
                     GFGTIRVGSLNSPLKNTGANVNAWESGKYTGELLEISKMAGREHRYLSARYDSPEFAG
                     FSGSVQYAPKDNSGSNGESYHVGLNYRNSGFFAQYAGLFQRYGEGTKKIEYDGQTYSM
                     PSLFVEKLQVHRLVGGYDNNALYVSVAAQQQDAKLYGATRVNSHNSQTEVAATAAYRF
                     GNVTPRVSYAHGFKGTVDDANHDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGADK
                     IVSTASAVVLRHKF"
BASE COUNT          698 a          821 c          650 g          536 t
ORIGIN      
        1 gtcggcacgg gaacctgcat cccgtcattc ccacaaaagt gggaatccgg aacgaaaaat
       61 ctcaagaaac cgttttaccc gataagtttc cgcactgaca gacctagatt cccgcctgcg
      121 cgggaatgac ggttcgggca ctcctgacag ggtgaattca caggatagcg attcgtagca
      181 actgcatcca accccccgaa caactcccca aacaacgccg cgcgccccgg gcgtttgccg
      241 tttccctgca aaatctgcga tacaatgcag tctgaacatt tatccgaatc ccaaacccga
      301 tggataccgc acaaaaacaa cgctgggcaa taaccctatc ctacgacggc agccgctttt
      361 acggctggca gaaacaggcg ggcggcgtac cgaccgttca ggcggcattg gaaaccgcac
      421 tcgcccgaat cgcaggagaa tctgtcgcca ccacagttgc cggcaggacc gacaccggcg
      481 tgcacgctgc cgcccaagtc gtccactttg acacaactgc cgcccgtccc caacaggcat
      541 gggtgcgcgg cgtaaatgcc cacctgcccg aaggcattgc cgttttgcac gcccgacaga
      601 ccgcacccgg atttcacgcc cgcttcgacg catacggacg gcactaccgc tacctgctcg
      661 aatccgcccc cgtccgttcc cccctgctca aaaacagggc gggctggaca cacctcaaac
      721 tcgacatcga accgatgcgc cgggctgccg ccttattggt cggcgaacaa gacttctcca
      781 gcttccgcgc cgccgggtgc caggcaaaat cccccgtcaa aaccatctac cgcgccgggc
      841 ttacccaaag cgcaggactg atccgcctcg atttgcacgg caacgccttt ttgcaccaca
      901 tggtacgcaa catcatgggc gcgctcgttt atgtcggcag cggcaggctc agcgtcgaag
      961 gcttcgccgc actgattcaa gaacgcagcc gcctcaaagc cccaccgacc ttcatgcccg
     1021 acgggcttta cctgaccggc gtcgactatc ccggggcata cggcatcgtc cgcccccaaa
     1081 tccccgaatg gctttaaaac attcttgtcg cggagatttt gaaatcggac aaactgtcag
     1141 gcaatctttt tcaatgttga cactatagcg gattaacaaa aatcaggaca aggcggcggg
     1201 ccgcagacag tacaaatggt acggaaccgg ttcgcccggt gcttgggcgc cttagggaac
     1261 cgttcccttt gggccggggc ggggcaacgc cgtaccggtt tttgttaatc cgctataacc
     1321 cccggtttgc aatgccctcc aataccctcc cgagtaagtg ttgtaaaaat gcaaatctta
     1381 aaaattttaa ataaccatat gttataaaac aaaaaatacc cgtaatatct ctatccgccc
     1441 ttcaaaatgc acatcggatt ccacacaaaa acaggcagaa gtttgttttt tcagacagga
     1501 acatctatag tttcagacat gtaaccgccg agcccctcgg cggtaaatgc aaagctaagc
     1561 ggcttggaaa gcccggcctg cttaaatttc ttaaccaaaa aaggaataca gcaatgaaaa
     1621 aatccctgat tgccctgact ttggcagccc ttcctgttgc ggcaacggcc gatgttaccc
     1681 tgtacggcgc catcaaagcc ggcgtacaaa cttaccgttc tgtaggacat caggaaggca
     1741 aagtagttgg cgtgggaacc ggcagcgaaa tctccgactt cggttcaaaa atcggcttca
     1801 aaggtcaaga agacctcggc aacggcctga aggccgtttg gcagttggaa caaggcgcct
     1861 ccgtcgccgg cactaacacc ggctggggca acaaacaatc cttcgtcggc ttgaaaggcg
     1921 gcttcggcac catccgcgtc ggcagcctga acagccccct gaaaaacacc ggtgccaacg
     1981 tcaatgcttg ggaatccggc aaatataccg gcgagcttct ggaaatcagc aaaatggccg
     2041 gacgggaaca ccgctacctg tccgcacgct acgattctcc cgaatttgcc ggcttcagcg
     2101 gcagcgtaca atacgcacct aaagataatt caggctcaaa cggcgaatct taccacgttg
     2161 gcttgaacta ccgaaacagc ggcttcttcg cacaatacgc cggcttgttc caaagatacg
     2221 gcgaaggcac taaaaaaatc gaatacgatg gtcaaactta tagtatgccc agtctgtttg
     2281 ttgaaaaact gcaagttcac cgtttggtag gcggttacga caataatgcc ctgtacgttt
     2341 ccgtagccgc acaacaacaa gatgccaaat tgtatggagc aacgagggtt aattcgcaca
     2401 actctcaaac cgaagttgcc gctaccgcgg cataccgttt cggcaatgta acgccccgcg
     2461 tttcttacgc ccacggcttc aaaggcactg ttgatgatgc aaaccacgac aatacttatg
     2521 accaagtggt tgtcggtgcg gaatacgact tctccaaacg cacttctgcc ttggtttctg
     2581 ccggctggtt gcaagaaggc aaaggcgcag acaaaatcgt atcgactgcc agcgccgtcg
     2641 ttctgcgcca caaattctaa tctgcaaaga ttggtatcaa caaaaagcct gtcgtcagac
     2701 aggct
//
LOCUS       DAWTCI010000124         2694 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWTCI010000124 DAWTCI010000000
VERSION     DAWTCI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.124"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(250..663)
                     /locus_tag="WM481_002059"
     CDS             complement(250..663)
                     /locus_tag="WM481_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081286.1"
                     /translation="MIHLNVHYCDEIIRHAENDKYSLIGIFPDICHIPTPQAILGRLC
                     LSVSFSAEGMDIQTMKTGQIFLEIVRNDDVISALEIPSYDGSDTEENVSFMLHQTISG
                     LPVSDNDRIYVRMTTHNHILSESRPLSFSWLPYHS"
     gene            complement(660..1121)
                     /locus_tag="WM481_002060"
     CDS             complement(660..1121)
                     /locus_tag="WM481_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9081287.1"
                     /translation="MGATLTVAVAACTVSTPTSAIPVTHIKCLRINGQIKCVKPISPN
                     TTPAAEHIEHVRKNPRRKAAMDRAAARIADKIALKAGGETFVSLRMKKGFTQSELATA
                     AGLPQPYLSRIENSKQSLQDKTVQKLANALGVSPLEVRAAFERRYEYMEQA"
     gene            complement(1138..1575)
                     /locus_tag="WM481_002061"
     CDS             complement(1138..1575)
                     /locus_tag="WM481_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081288.1"
                     /translation="MELTVHFNAEQDLDRLFEKDEEAVGYLENVIAMIQADSAIFDGL
                     YKNRYFREYGEPIGPIDLEVKPILSLWGKDIKVLRVRFDSEEAAGYRIIYAPCHEKQP
                     NGTYIRRIDILAVVNKKTDEFDYQAEHPITKRIIKDYEELYSN"
     gene            complement(1688..2404)
                     /locus_tag="WM481_002062"
     CDS             complement(1688..2404)
                     /locus_tag="WM481_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFA9081289.1"
                     /translation="MPFIIDYQCLCAINSEIKMFSGEQLGQAISEAIKRKNVSQKEVA
                     DHFGVKQPSVSGWIKNGRIDKKHLDKLIDYFSDVVTPSHFGIETFRVLKSNEQSSIRF
                     PRLNAEATCGAGTINDHYIEVVDYVTVAAAWAREKLGGNLNKIQVITARGDSMEPTIE
                     NGDVMFVDTAVEAFDGDGLYLLWYIDGLKAKRLQSTVGGGLMIISDNSSYRTETVRGE
                     DLNAVRIIGRIRGAWRLSQF"
     gene            2473..>2694
                     /locus_tag="WM481_002063"
     CDS             2473..>2694
                     /locus_tag="WM481_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9081290.1"
                     /translation="MAMSIQKAVDYFGNESRLARAIGVKQPTVWAWNKKGTPPPIIRC
                     VQIEKLTGGAVNRKDLRPDDWHLIWPELAG"
BASE COUNT          645 a          582 c          643 g          824 t
ORIGIN      
        1 cgtcctttgc cctcgcgcca aaccgattta ttctgctgat taattcgttc atcccgtttc
       61 ctttaagtgg ttgtttgttt cgatgggtgt attataacta ttacctatat ttaatcaata
      121 ggcaatagtt atattttcat ccatatatac ttattgaatt gatttttaga agaaaaaagt
      181 ttgaaaaaaa accgcccgaa tggcggtgta tcatgaaaaa gaaaaccgcc ctagggcggt
      241 tcggtattgt tatgagtggt aaggaagcca agaaaaagac aaaggacggc tctcggagag
      301 aatatggttg tgtgttgtca ttcttacata aatgcggtcg ttgtctgaaa cgggtagtcc
      361 gctgattgtt tggtgtagca tgaatgacac gttttcttct gtatcactgc cgtcatatga
      421 tggaatctca agtgcagaga ttacatcatc gttgcggacg atttccagaa aaatttgacc
      481 ggttttcata gtctgtatat ccatgccttc ggcggaaaat gaaacagaca ggcacaggcg
      541 gcctagaatg gcctgcggtg tcggaatatg gcatatatcg gggaatatac ctatcaggct
      601 gtatttgtca ttttccgcat ggcggataat ttcatcgcaa tagtggacat ttaagtgaat
      661 catgcctgct ccatatattc gtaccgccgt tcgaacgctg cgcgaacttc aagcggggaa
      721 acgcctaatg cgtttgccaa tttttgtaca gtcttatctt gcagggattg tttgctgttt
      781 tcgatgcggg acagataggg ttgaggcaat cctgcggcgg ttgccaattc ggactgggta
      841 aacccctttt tcatccttaa gctgacaaat gtttccccgc ctgctttgag ggctatttta
      901 tcggcaattc ttgctgccgc cctgtccatc gctgctttgc ggcgcggatt tttccgcaca
      961 tgctcaatat gctctgcggc aggggtagtg ttagggctta tcggtttgac gcatttaatc
     1021 tgcccgttta tccgcaggca ttttatgtgg gtgacgggga tggcggatgt aggtgttgat
     1081 actgtgcagg cggccacggc aacagtgagt gttgcaccca ttttgaacca tgtgccgtta
     1141 gttggagtac agttcttcat agtctttgat aatccgtttt gtgattgggt gttctgcctg
     1201 atagtcaaat tcgtctgtct tcttgtttac aacagccaat atatctatgc ggcggatata
     1261 cgtgccgttt ggctgttttt catggcatgg tgcgtaaatt attctgtatc cggcggcttc
     1321 ttcgctgtca aatcggacgc gtaaaacttt gatgtctttt ccccataatg acaatatggg
     1381 tttcacttcc aagtcaatcg ggcctatggg ttcgccatat tccctgaagt atctgttttt
     1441 gtataagccg tcaaaaatag cagagtccgc ctgaatcatc gcaatgacat tctcgagata
     1501 accgaccgct tcttcgtctt tttcaaagag gcggtctaaa tcctgctcgg cgttaaagtg
     1561 gacggtcagc tccatgagtg ccctttttat atatatatta tatcttttta gttataccgc
     1621 gcaatatgcg gtaataaatc agttgttcaa gtattttaat ccggcacgct ccaccagaag
     1681 acgcggtcta gaactggctc aaacgccatg cgccgcgtat gcgtccgatg atgcgtacgg
     1741 cgtttaaatc ttcgccgcgc acggtttcgg ttcggtatga gctgttgtcg ctgatgatca
     1801 tcaggccgcc gccgacggtg gattgcagcc gcttggcctt aaggccgtct atataccaaa
     1861 gcaggtagag gccgtcgcca tcgaaggctt cgacggcggt atcaacgaac attacgtcgc
     1921 cgttttcgat ggtgggctcc atgctgtcgc cacgggctgt aatgacttgg attttgttga
     1981 ggtttccgcc cagtttctcc cgcgcccatg cggcagcgac ggttacataa tccacaacct
     2041 cgatatagtg gtcgttaatc gtgcctgcgc cgcaggtcgc ttcggcattt aagcggggga
     2101 aacgtatgct actttgttca ttcgatttta agactctgaa tgtttcaatg ccgaaatggc
     2161 ttggcgttac tacgtctgag aaataatcaa ttaatttatc tagatgtttt ttatctattc
     2221 gtccattttt tatccaacct gaaacgcttg gctgtttcac cccaaaatga tcggcaacct
     2281 ccttttgact gacatttttt ctcttaatcg cttctgatat tgcttgtcct aactgttcgc
     2341 ctgaaaacat tttgatctcc gaattaattg cgcataagca ttggtaatcg ataataaaag
     2401 gcataaccta taaaaggcaa tagttgtatt taatataagt attagctata atgaagctat
     2461 ttaattgagt aactggctat gagtatccaa aaagcagttg attattttgg taatgaatcc
     2521 cgacttgcac gggcgatcgg agttaaacaa ccgacggtgt gggcttggaa taaaaaagga
     2581 acgccgcccc cgatcattcg gtgcgtgcag attgaaaaat taaccggagg cgcagtgaat
     2641 cgaaaagact tacgtcctga tgactggcat ctaatctggc cggaattggc aggc
//
LOCUS       DAWTCI010000125         2642 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWTCI010000125 DAWTCI010000000
VERSION     DAWTCI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.125"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..146
                     /locus_tag="WM481_002064"
     CDS             <1..146
                     /locus_tag="WM481_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081291.1"
                     /translation="LRLLQQTACRFKAALETAAWKHYVRAIKESEPVPDAEARRKRKK
                     QAA"
     gene            172..366
                     /locus_tag="WM481_002065"
     CDS             172..366
                     /locus_tag="WM481_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081292.1"
                     /translation="MDSEYLDPQQCADILSVKKRTFLERYAPRPDFPARISVSKKRFW
                     WKKEEVEGWLDRQKEKRPMM"
     gene            complement(422..506)
                     /locus_tag="WM481_002066"
     tRNA            complement(422..506)
                     /locus_tag="WM481_002066"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Leu,seq:tag)
     gene            657..1331
                     /locus_tag="WM481_002067"
     CDS             657..1331
                     /locus_tag="WM481_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081293.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGMASIDRSV
                     LWCNDEGWKLADYLPCDDVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENAALR
                     LVCLSQQGEGLENIWEQDGNITDVSLACRSAQSGWMNVASDVRRWLNLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADTAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1324..1917
                     /gene="ribA"
                     /locus_tag="WM481_002068"
     CDS             1324..1917
                     /gene="ribA"
                     /locus_tag="WM481_002068"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFA9081294.1"
                     /translation="MSELLDHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNCSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQAEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQEQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENERYLQTKADKLGHLMSE"
     gene            2085..2267
                     /locus_tag="WM481_002069"
                     /pseudo
     CDS             2085..2267
                     /locus_tag="WM481_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            2270..>2642
                     /locus_tag="WM481_002070"
     CDS             2270..>2642
                     /locus_tag="WM481_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFA9081295.1"
                     /translation="MRNAVGLDISKLTFDATAIVGNAEYSAKFDNDSKGLNQFSDRLK
                     SLGCQNLHICMEATGNYYEEVADYFAQYYSVYVVNPLKISKYAESRFKRTKTGKQDAK
                     LIAQYCRSAQESELVKRQKPTD"
BASE COUNT          671 a          596 c          746 g          629 t
ORIGIN      
        1 aactgcgcct attgcaacaa accgcctgcc gcttcaaagc cgccctcgaa acggccgcgt
       61 ggaaacacta cgtccgcgcc atcaaagaat ccgagccggt accggatgcc gaagcgcgcc
      121 ggaagcggaa aaaacaggcg gcgtaaatat aattaagttt ggaggtttta aatggatagc
      181 gaatacctag acccgcagca atgcgcagat attttatccg taaaaaagcg tacattttta
      241 gagcggtacg caccgcgtcc ggatttcccc gcgcgaatat ccgtatcgaa gaaacgtttt
      301 tggtggaaaa aagaagaagt cgaaggatgg ctagaccgcc aaaaagaaaa acgcccgatg
      361 atgtgatttg caccacattt gcaccaagtg gtatataaca ttatgaaaga tataaaaatt
      421 ttggtgcgga cggagagact cgaactctca cacctctcgg cgccagaacc taaatctggt
      481 gcgtctacca atttcgccac gtccgcataa gggtggagat tatacagatt ttgtccgatt
      541 gcgcaaggct ttggacgtaa tagttgaggc ttatgctttg cagcggtaaa atccgctatt
      601 cgtccgcctg gcatcggaat cgggcggttt tttgttttta ttgacggaat ttgggtatgc
      661 ctgctgcttt gattaaggat tttctgctga ctcagggttt gaagctgccg cttgacgagg
      721 ttcgggcggc gtatctgacg gcgcagacgg taatggatat ggggatggct tcgattgacc
      781 gttctgtttt gtggtgcaat gatgagggtt ggaaacttgc cgattacctg ccgtgcgatg
      841 atgtccgcga agatgccctg aaacggcttt ttatggcttt ggattcggtg ttttcacgct
      901 cgacaggtgt gcggagcgcg gcggtttatg ccttgatgcc gtctgaaaac gccgccctcc
      961 ggctggtgtg cctgtcccaa cagggcgaag gtttggaaaa catatgggag caggatggaa
     1021 atattaccga tgtttcgctt gcctgccgtt cggcgcaaag cggttggatg aatgttgcct
     1081 cggatgtacg ccgttggttg aatttgggtg agctttcggg agaacgcaat catgcttcgg
     1141 cggcgcaaat ttccattccc gtttgcacgg agagcggcgg tgtattgggc gtggttcatg
     1201 tggaatttga atgcgcagag tgtgcggata cggcggcgca ggcggaatgg gtggctcttg
     1261 ccttggcttt gtccgaacct ttgaagcaac ttttgggcat cactgccgca gaaggagatg
     1321 aaaatgtctg aattattaga ccatgtcgct tcctgccgtc tgccgaccga atggggcgta
     1381 tttacgatgc acggttttga agaggcaaac gggcaggaac acgtcgcgct gaccgtcggc
     1441 aattgttcag acggcaatcc ggtgttgacg cgcatccact ccgaatgtct gacgggcgac
     1501 gcgctgttct cgagaaaatg cgactgcgga ccgcaacttg aagcggcaat gagggcggta
     1561 caggcagagg ggcgcggcat catcgtctat ctgcgtcagg aaggacgcgg catcgggctg
     1621 attaacaaaa tccgcgccta tcatctgcaa gaacaaggta tggataccgt tgaagccaat
     1681 ttggcactcg ggctgcccgt cgatgcccgc gatttccgtt tggcgcaatc tatctacgaa
     1741 tatctgggca tccgctcggt caaactgttg accaacaacc ccgaaaaaat ccaaaccctg
     1801 aaagatgcgg ggattaacgt ggtcgaacgc attcccctgc acgtcgggga aaatctggaa
     1861 aacgagcgtt atctccaaac caaagcagac aagctggggc atttgatgtc ggaataaggc
     1921 aaagttgcag ggaacgggca tcctgcgccg tctttcggga aacaggtttc catagcttga
     1981 taaagcaata agttttatca agttgcaggg tgcggatgca aacgcattgc gagcgcgggt
     2041 ttgaggcata cgcgcaaaca tcttaataca acgtattgat atttatgatt ttctctatca
     2101 tcgtccctat ttacaatgtg gaaaaatacc ttcgctgctg cgtggattcc gtgcttgccg
     2161 aaaattttgc cgattatgaa atgattttgg tcgatgacgg ttcgccggac ggctgcggga
     2221 agatttgcga cgaatatgca ggcaaatatc cgcatataag ggtaatccca tgcgtaacgc
     2281 cgtaggattg gacatatcca agctgacatt tgacgcaacg gccattgtcg gcaatgccga
     2341 atattcggca aagtttgaca acgattcaaa aggtttaaat cagttttcgg accggttgaa
     2401 aagcttggga tgtcagaatc tgcatatctg catggaggca accggcaatt attatgaaga
     2461 agttgccgac tacttcgcgc agtattacag cgtttacgta gtgaatccgc tgaaaataag
     2521 caagtatgca gaaagcaggt tcaagcgaac caaaacaggc aaacaggatg caaagctgat
     2581 agcgcagtat tgccggtcgg cgcaggaaag cgagcttgta aagaggcaga agcctacgga
     2641 cg
//
LOCUS       DAWTCI010000126         2616 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWTCI010000126 DAWTCI010000000
VERSION     DAWTCI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.126"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(46..705)
                     /locus_tag="WM481_002071"
     CDS             complement(46..705)
                     /locus_tag="WM481_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFA9081296.1"
                     /translation="MTPPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRALLEMPSEAAVADIARTYSAHYLNPNNRNMTLFPDAPPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGLCGELGLDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLGAPHLAVLDGLSELPGFLARHY
                     A"
     gene            865..1080
                     /locus_tag="WM481_002072"
     CDS             865..1080
                     /locus_tag="WM481_002072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081297.1"
                     /translation="MSVFRINMTAATVLAALSSPVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSLKAAIWMTASIPASPPAA"
     gene            1026..1569
                     /locus_tag="WM481_002073"
                     /pseudo
     CDS             1026..1569
                     /locus_tag="WM481_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            1639..1959
                     /locus_tag="WM481_002074"
     CDS             1639..1959
                     /locus_tag="WM481_002074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081298.1"
                     /translation="MRSQPEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMQSEGYPVLFVLDGEAFFPRCTT
                     SCSR"
     gene            1947..2570
                     /locus_tag="WM481_002075"
     CDS             1947..2570
                     /locus_tag="WM481_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFA9081299.1"
                     /translation="MQSLMNNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGD
                     NATADERRQFGQADRFADFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSH
                     RCFRRHWLVSPSIWWHNRRILDFMPSENRLDGIDACLNIGALERSSGCKRREERDMAG
                     QAEQMAAELDRRGTAVFFREYPNADHGNVPFYSLTDCVEYLREAWQR"
BASE COUNT          606 a          759 c          734 g          517 t
ORIGIN      
        1 aggcttcaga cggcattttt gccgcgtgcc ggatgcggaa accaatcagg cgtaatgtcg
       61 tgcaagaaaa ccgggcagtt cggacaaacc gtccaatacg gcgagatgcg gtgcgccaag
      121 gagctgttcg cgcgaatgtg cgccggtggc aacgccgact gccgccgcgc ctgcgtttgc
      181 cgccatatgc aggtcgtgcg ccgtatcgcc gacaaccaat gcctctttcg ggtcgagtcc
      241 cagttcgccg cagagtccga ataccatttc gggcgagggt ttggaggggt attcccccgc
      301 gcaggcggtg gcgagccaat agccgccggt ggcggtttgg ctgatggcgt tgtccaaacc
      361 cgcccgccct ttgcccgtgg cgacggcaag ccggaagcct tgcgctttga gcttgtccag
      421 acagggcggg gcatcgggaa agagtgtcat attgcggttg ttgggattga ggtaatgcgc
      481 ggaataagtg cgcgcgatgt cggcaacggc agcttcagaa ggcatttcga gcagggcgcg
      541 gatgatttcg ggcaggctgt atccgatcag gctgcggacg cgctccgctt cgggcggcgg
      601 aaaaccgcat tcggcgaagc tgcggcgcat ggtgtcgatg atgggttggg tcgtatcggc
      661 aagcgtgccg tcccagtcga agatgatgag tttgggcggg gtcatggcag gttggttgca
      721 gtaaaaaagc agattttatg cggaaaacgc agacgtgtcg cattttcgac aaaatttgtc
      781 ggctgagcga tatgtttttc cgaacaagcc gcgttgtgct ttattaaaat agaaccatta
      841 tcatttatac agatgggaca gtttatgtca gttttccgca tcaatatgac cgccgccacg
      901 gttttggcag cactctcgtc tccggttttt gccgcacaaa cggcggattt ggaaaccgtc
      961 catatcaaag ggcagcgttc gtacaacgcg attgccaccg agaaaaacgg cgattacagc
     1021 tcgttaaagg cagctatatg gatgaccgcc tcaatacccg cgtctccccc tgccgcctga
     1081 aagacaaaaa cgccgccgaa cccgaacaac cgcaacaccc gttacgccgc attgggcaaa
     1141 cgcgtgatgg aaggcgttga gaccgaaatc agcggtgcga ttacaccgaa atggcaaatc
     1201 catgcaggtt acagctatct gcacagccaa atcaaaaccg ccgccaatcc acgcgacgac
     1261 ggcatcttcc tgctggtgcc caaacacagc gcaaacctgt ggacgactta ccaagttacg
     1321 cccgggctga ccgtcggcgg cggcgtgaac gcgatgagcg gcattacttc atctgcaggg
     1381 atgcatgcag gcggttatgc cacgttcgat gcgatggcgg cataccgctt cacgcccaag
     1441 ctgaagctgc aaatcaatgc cgacaacatc ttcaaccgcc attactacgc ccgcgtcggc
     1501 ggcacgaaca cctttaacat tcccggttcg gagcgcagcc tgacggcaaa cctgcgttac
     1561 agtttttaaa gaccaatatg ccgtctgaaa cggcagccgc agcataatca aaccacaaca
     1621 agctgcgcgg cataccctat gcgctcacaa ccggagtatg gcattgcgaa ggaaaacaga
     1681 ccgaaccggc aggcagaccg ctttgccggt tcggttttac cgcttgccgc cagtctgacc
     1741 cacaagccga acatcatgaa acccataccg accgacacat tccaacctgc catactgccc
     1801 caagcctttg aaaccgaaat caaatccacc tgcacggggc gaatctatcg gattcagacg
     1861 gcaacactcg gcgaaatgca gtctgaaggc tatcccgtcc tctttgtcct cgacggcgaa
     1921 gcctttttcc cgcgctgtac aacatcatgc agtcgctgat gaacaacccc gttacccgaa
     1981 gcaacgcccc ctgcctgatt gtcggtatcg gctacacgac aggcagtgtg cgcgacctgg
     2041 cgcagcgtgc cgccgactac acgccgccgc ttggagacaa cgccacagca gacgaacggc
     2101 ggcagttcgg acaggcagac cgcttcgccg actttatcga cagcgaactg accgcctttt
     2161 tagaaagccg ctacaccctt aaccgtaatg aaaccgccgt attcggacac tcgttcggcg
     2221 cactgttcgg actctattcc ctgctttccc accgctgttt cagacggcat tggctcgtat
     2281 ccccctcgat ttggtggcac aacaggcgga tactcgactt tatgccgtct gaaaaccggc
     2341 tggacggcat cgatgcctgc ctcaacatcg gcgcactcga gcggagcagc ggttgcaaac
     2401 gcagggaaga acgcgacatg gcagggcagg ccgaacaaat ggcggcagag ttggacaggc
     2461 gcgggaccgc cgtatttttc cgggaatatc cgaatgccga ccacggcaat gtcccgtttt
     2521 actcgctgac cgactgcgtc gaatatttga gggaagcgtg gcaacggtag ggggaatcaa
     2581 atatatgact gctttgtttt gcatcggaaa atatag
//
LOCUS       DAWTCI010000127         2560 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWTCI010000127 DAWTCI010000000
VERSION     DAWTCI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.127"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            432..>2560
                     /locus_tag="WM481_002076"
     CDS             432..>2560
                     /locus_tag="WM481_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9081300.1"
                     /translation="MNKTLKRRVFRHTALYTAILMFSHTGGGGAMAQTHQYAIIMNER
                     KQPEVKWKDQYNQSALKDKSRERTFSHTSQKNGLGITSNFISFNNNDELVSQQSGTAV
                     FGTATYLPPYGKVSGFDTAELNKRGNAVNWIHTTRPGLAGYGYTGIRCGSTKDCPKLT
                     YKTRFSFDNPDLLKTRGGLDRHTEPSRENSPIYKLKDYPWLGVSFNLGGEGTAKDGRS
                     SSKLVSSFDENNSNSNQNLVYTTEDHRISLSNWQQETTAMAYYLNAKLHLLDKKGIKD
                     ITGKTVRLGVLKPSIDVKTQNTGLSGILNFWSKWDIKDNGQIPVKLGLPEVKAGRCIN
                     EPNPNKNTQAPSPALTAPALWFGPGQNGKVQMYSASVSTYPGSSSSRIFLQELKTQTD
                     PARPGRHSLAALNARDIKSREPNFNSRQTVIRLPGGVYRIGPTRNGIVGLNGNDGKND
                     TFGIYKDRLVTPEVDEWAKVLLPWTVRYYGNDDIFKTFNQPNNKKQSDKKQYSQKYRI
                     RTKEDDNDKPRDLGDIVNSPIVAVGGYLATSANDGMVHLFKRNGTNQRGYELKLSYIP
                     GTMERKDIENQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRRITDDQDREKHFFMFGA
                     MGLGGRGAYALDLTKIDSNNLTGVSMFDVQNDKNNNNNDSNRVKLGYTVGTPQIGKTQ
                     NGKYAAFLASGYAAKDIVSSDNTTALYV"
BASE COUNT          703 a          704 c          660 g          493 t
ORIGIN      
        1 tgccgcctcc ccggcccgcc attgccgcgc aggcggcaat ggcgggccgg gcaaacggtt
       61 gcggattgcc gaaaaccgct ttaccatgat cagcgcgcaa gggtagaata atgcggtaac
      121 cctatacatt gcatccggcc aaaggggcgt gttacctttg cgaacatccc cctttggcag
      181 ccgggcgaag ggggctttgc aaccggcaat ccggcgggcg cgggatcggg cggtttgccc
      241 aatcccgccg tttgcggcgc gcctgccgcc gacggtatcc cgcgaagcaa gatttaaggg
      301 ataaaatatg ttccaacacg cagggcggca cataaggcgc cgccctgatt cggaagggct
      361 tgcacccctc ccggacaaag cctgatcctg ccgccccgaa gggcgggggg tttgaccgaa
      421 aaggaaatac gatgaataaa actttaaaaa ggcgggtttt ccgccatacc gcgctttata
      481 ccgccatctt gatgttttcc cataccggcg gggggggggc gatggcgcaa acccatcaat
      541 acgctattat catgaacgag cgaaagcagc ccgaggtaaa gtggaaggat caatataatc
      601 aatcagcatt aaaggacaaa agcagggagc ggacatttag ccatacgagc cagaaaaacg
      661 gcctcggcat cacaagcaat tttatctcat tcaacaataa cgatgagctt gtttctcaac
      721 aaagcggtac tgccgttttt ggcacagcca cctacctgcc gccctacggc aaggtttccg
      781 gctttgatac cgccgagctg aacaagcgcg gcaatgccgt caattggatt cataccaccc
      841 ggcccgggct ggcaggctac ggctacaccg gtatccgttg cggaagtacc aaagactgtc
      901 ccaaacttac ctataaaacc cgattttcct tcgataatcc cgacttgcta aaaacaagag
      961 gcgggctgga taggcacaca gagccaagcc gcgaaaattc gcccatttac aaattgaagg
     1021 attatccatg gttgggtgtg tctttcaatt tgggcggcga gggtaccgcc aaagatggca
     1081 gatcatccag caaattggta tcttcttttg atgaaaacaa tagtaatagt aatcaaaacc
     1141 tcgtctatac gacagaagac caccgtattt cccttagcaa ctggcagcaa gaaacgaccg
     1201 ccatggccta ttatctgaac gccaagctgc acctgctgga caaaaaaggg attaaagata
     1261 tcaccggcaa aacagtgcgg ttgggtgtct tgaagccgag catcgatgtg aagacacaaa
     1321 atacggggct tagcggcatt ctaaattttt ggtctaagtg ggacattaaa gataacgggc
     1381 agattccggt caagctcggc ctgccggaag tcaaagcagg ccgctgcatc aacgaaccga
     1441 accccaataa gaatacccaa gccccttcgc cggcactgac cgcccccgcg ctgtggttcg
     1501 gccctgggca aaatggtaag gtgcagatgt attccgcttc ggtttctacc taccccggca
     1561 gttcgagcag ccgcatcttc ctccaagagc tgaaaactca aaccgacccc gcccggcccg
     1621 gccggcattc cctcgccgct ttgaatgcgc gggatatcaa atcccgcgag ccgaatttca
     1681 actcaaggca gaccgtgatc cgattgccgg gcggcgtgta ccggatcggc ccgactcgca
     1741 acgggatcgt gggtttgaat ggcaatgacg gcaaaaacga cactttcggc atctacaagg
     1801 acaggctcgt cacacctgag gtcgacgagt gggcaaaagt gctgctgcct tggacggtcc
     1861 ggtattacgg taatgacgat atatttaaaa cattcaacca accaaacaac aaaaaacaaa
     1921 gcgacaaaaa acaatacagc caaaaatacc gcatccgcac aaaagaagat gacaatgaca
     1981 aaccccgcga tttgggcgac atcgtcaaca gcccgattgt cgcggtcggc gggtatctgg
     2041 caacttctgc caacgacggg atggtgcacc tgtttaaaag aaacggcaca aaccaacgag
     2101 gctacgaact gaagctcagc tacatccccg gtacgatgga gcgtaaggat attgaaaacc
     2161 aagactccac cctcgccaaa gagctgcgcg cctttgccga aaaaggctat gtgggcgacc
     2221 gctacggcgt ggacggcggc tttgtcttgc gccgcattac agatgaccag gacagggaaa
     2281 aacatttctt tatgtttggt gcgatgggcc ttggcggcag aggcgcatac gccttggatt
     2341 tgaccaaaat cgacagcaac aacctgaccg gcgtttccat gtttgatgtc caaaacgaca
     2401 aaaataacaa taacaatgac agtaatcgcg tgaaattagg ctacaccgtc ggtacgccgc
     2461 aaatcggcaa aacccaaaac ggcaaatacg ccgccttcct cgcctccggt tatgcggcta
     2521 aagatattgt cagcagcgat aatacaaccg cgctgtatgt
//
LOCUS       DAWTCI010000128         2515 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWTCI010000128 DAWTCI010000000
VERSION     DAWTCI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.128"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(84..473)
                     /locus_tag="WM481_002077"
     CDS             complement(84..473)
                     /locus_tag="WM481_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFA9081301.1"
                     /translation="MNPPEKLLTAENPALRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHAEQAVYDHARTVYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQPAR"
     gene            complement(636..1015)
                     /locus_tag="WM481_002078"
                     /pseudo
     CDS             complement(636..1015)
                     /locus_tag="WM481_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1069..2202)
                     /gene="carA"
                     /locus_tag="WM481_002079"
     CDS             complement(1069..2202)
                     /gene="carA"
                     /locus_tag="WM481_002079"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFA9081302.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSASGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSSFRASESLHDYL
                     VRNETVAIADIDTRRLTMLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDKQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKQA"
BASE COUNT          517 a          627 c          718 g          653 t
ORIGIN      
        1 gcttttcaga cgaccttttt aaacgctcaa ttcagttaat tttaccttat tattttcaaa
       61 atctaatcaa aatcaaccgt ttgctaccgt gctggctgct tttccaattc ctgcaatacg
      121 cgcaggattt ccgccagtac atcgtttgtc tgctgcaaaa tttcgtgatt ccaaaaacgc
      181 agcacggtaa agcccaagct gttgagatat accgtccgcg cgtggtcgta tacggcttgt
      241 tccgcgtgct gcccgccgtc tgcttcgaca atcagcttgg gcgttacgca cataaaatca
      301 acaatataat tccccatcgg ctgctgtcgg cggaatttat agccgttcag acggcctgcc
      361 cgcaggtgtt gccacaattt tgcttccgcc tcgctcattt cttgacgcat ggctttggcg
      421 cgttggcgta gggcggggtt ttcggcggtc aatagtttct cgggcgggtt catttttgtt
      481 atcgggttgc tgtttgtgta agccttacag cttgttgccc tctctctaac tctttcccac
      541 agggagagag gacgggtcgg ctgttggggt taaaggggct gcaaggaaaa cagcttgtgc
      601 aggctgtttt ttattttgca taatccgtct gaaaattaat aactgctgat ttccagcagg
      661 ttgccatcgg gatcgcgcag gtaaaccgat tggattttgc ccattgcgcc tgtgcgcgct
      721 acgatgccgc ttaaaggttt gatgccgtgt gcggataatt cctgtaaaac cgtttccagt
      781 ggggcgtatc ggtcagcagg cataaatccg ctgtgccgca ggcggcgtgt tgcgcgttag
      841 gctgaatttc cgcaccgcgc ccgtgtaggt tgattttctg actgccaaac aacaaagctt
      901 tacggttgtt gccaaatgaa acttcttcca tgcccaaaac ttgtgtgtaa aacgcgatgg
      961 ttcggtcaat gtcggcaaca gtcagtacta gatggtcgag tgcgctaatt ttcatttcca
     1021 gctttctttt tcagacggca gcagcatact gttgccgtct gaaaatcatt atgcttgttt
     1081 tgcagccttc atattgccaa tgaatttgtc gaacaaatag ccgacatctt gcggacccgg
     1141 gctggcttcg gggtgtcctt ggaaacagaa cacgggtttg tcggtcagct cgatgccttg
     1201 caaggtattg tcgaacaagg atttgtgggt aatgcgtgcg ttggcgggca gggtgtcggc
     1261 atcgacggcg aaaccgtggt tttggctggt aatgacgact ttgccgctgt ccaaatcttg
     1321 cacaggatgg ttcgcaccgt ggtggctgaa gcgcattttc agggttttcg cgccgatggc
     1381 aaggctgatg agctggtgtc ccaagcagat gccgaaaatc ggtttgccgc tttccatcag
     1441 tttttgcacg gcttcgatgg cgtaggtgca aggctcgggg tcgccggggc cgttggacag
     1501 gaacacgccg tcgggattga gtgccaacac gtcttccgcg ctcgtttgtg cggggacgac
     1561 ggtcaggcgg cagccgcgcg aggcgagcat acgcaggatg ttggttttca cgccgaaatc
     1621 gtaggcgacg acgtggtaag gctgtttgtc aggggtaacg aaacctttgc ccaattccca
     1681 ttcgccttcc gtccattcgt aagtttccgt gcaggaaact tcttttgcca aatctttgcc
     1741 gaccatgctg ccgaacgcgg cgatgagttc ttgcgctttt tcaacggtgg catccgcacc
     1801 tgtcagaatc gcgccgcctt gcgcgccttt ttcgcgcaac agcatggtca ggcggcgggt
     1861 gtcgatgtcg gcgatggcga cggtttcgtt gcgtaccaaa tagtcgtgca ggctttcgga
     1921 ggcgcggaag ctgctgtgca agagcggcag gtcgcggata atcaggccgg cggcataaac
     1981 gctgcggctt tcttcatctt cggcgttggt gccggtgttg ccgatgtggg ggtaggtgag
     2041 ggtaacgatt tgtttgcagt aggacgggtc ggtcagaatt tcctgatagc cggtcatcga
     2101 agtattgaac acgacttcgc cggaagccga accttcgtaa ccgattgatg tgccgtggaa
     2161 tacgctgccg tcagcgagga cgaggagggc gggggtgctc atgatgggaa tcctgttttt
     2221 aataaaattc ttgacaatgc cgtctgaagg gacttcggca gggggcatcc ggtcggaaaa
     2281 atccggtcaa aaaaaaacac gccgcgcaag atagacgcgt aacgtgcttt tcgggcaagt
     2341 gcctataccc tgaaagcagc atattttaag acgaaacata cccgatttca agcgcaaacg
     2401 cggcaggcgg gctgatatgt tcgcgcgaaa acggaaatcc ggggcataaa accgcccgct
     2461 ccgcgccccc tatgcccgtt acgcatttcc ccacgtccgc ccggcgaaac tatgg
//
LOCUS       DAWTCI010000129         2444 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWTCI010000129 DAWTCI010000000
VERSION     DAWTCI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.129"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(161..934)
                     /locus_tag="WM481_002080"
     CDS             complement(161..934)
                     /locus_tag="WM481_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFA9081303.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
     gene            complement(1034..1657)
                     /gene="rsmG"
                     /locus_tag="WM481_002081"
     CDS             complement(1034..1657)
                     /gene="rsmG"
                     /locus_tag="WM481_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFA9081304.1"
                     /translation="MERKERLRAGIAAMGPDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLRQAAIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1752..2231)
                     /locus_tag="WM481_002082"
     CDS             complement(1752..2231)
                     /locus_tag="WM481_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081305.1"
                     /translation="MFVNEKYPYATLFAGLVFLTLPFALAVHDAFACAFGRAGLLVSV
                     SAGGFGRRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAVAWIFKLLLGSAALGGLSGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRKK"
     gene            2338..2413
                     /locus_tag="WM481_002083"
     tRNA            2338..2413
                     /locus_tag="WM481_002083"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2371..2373,aa:Thr,seq:cgt)
BASE COUNT          557 a          844 c          574 g          469 t
ORIGIN      
        1 ccttagggaa tcgttccctt tgagccgggg cggggcaacg ccgtaccggt ttttgttaat
       61 ccgctataaa tgacgatatt aagtattttt attttaatcc gccatattaa cgcacccggc
      121 caaacagcat aaaggcacgg gcagcccgat ttggattgac ctatttcccc gacaccctcg
      181 ccgccagctc gtccgccaag gcaagatacg ccttggcacc ctttgcctgc gcgtcgtaag
      241 ccatcaccgg cataccgtgg ctcggcgctt ccgcaaggcg gatattgcgc gggatgacgg
      301 tttcaaaaag caaatccccg aaatggctgc gcaactgttc gctgacttcg gcaaccagcc
      361 tgctgcggct gtcgtacatc gtgcgcacga tgcccgtgat gtccaaatcg ggattgaccg
      421 cctgacggat tttgcgcacg gtcgcaatca aatcggaaat cccttccagc gcgtaatatt
      481 cgcacaacat cggcacaatc acgccgcccg ccgccaccaa gccgttaagc gtcaacagcg
      541 tcagcgaagg cggacagtcg atcaggataa agtcgtaatc ttccgccact gccttgagcg
      601 cgtttttcaa acgcacttcc cgggcgattt cctgcaccag ctcgatttcc gcgccggcaa
      661 gcgcgcggtt cgcacccagc acagcgtatc cgccctcttt gctgcgtacc gccgccgact
      721 gcacgtccgc atcgcccaat aagacctgat aaacgccgga ctgcaaaccc gccttgtcga
      781 tgccgctgcc cgtcgtcgca ttgccctgcg gatccaaatc gaccaccagc acgcgtttgc
      841 cgcgcgatgc cagcgaagcc gccaaattta ccgtcgtcgt cgttttgccc acaccgccct
      901 tctgattggc gatggcaagg atgttcgcac tcatgtcccg cccgtgtccc gtctgatgtt
      961 gaaaaaaaga aaaacagtgc ggaattttac ctttatccgc acaaaaaagg tattcatgcc
     1021 gtctgaagcg cgctcaacgc ttgctcagga tgacgatatg gcgttccgca tccaagcccg
     1081 gcacgtcgag cctttggact ttttcgacgc acacatcctg cggcaggcgg ccgatttccc
     1141 cctgcggata tacgcccttc atcgccgccc agtagccgcc gtctttcaac aggtgcgccg
     1201 tccacgacac aaaatccgcc agttccgcaa acgcacggct ggtaaccacg tcggcacgca
     1261 cgtccgaaac cgcctccacg cgccccgaca ccacgcgcac attgtccaac cccaactcga
     1321 tagccgcctg ccgcaaaaaa gccgttttct tcgtattcgc atccaacaag gttatctgca
     1381 catccggacg gcacaccgcc gccggaatgc cgggctggcc gccgcccgaa ccgacatcca
     1441 gcatcgtctg tgcgccttcg atatagggca gcagcgtcag gctgtccaaa agatgatgga
     1501 caatcatttt ttcctcgtcg cgcagggcgg tcagattgta ggttttgttc cactttttca
     1561 acaaatccac ataggccaaa agcctgtcct gcgccgtttc cgaaatatcc ggccccatcg
     1621 cggcaatgcc tgcacgcagg cgttctttac gttccatatc tgcttcctgt ccatattcag
     1681 gcgtaatgtt agcggaaaaa catgccgtct gaaaccgcgt atcgggtttc agacggcatt
     1741 tccgttccgt cctatttttt cctgtggaac accacaggct gcaccggcgc gcgcgtcagg
     1801 cgtttcggca gcgtcagcat agacaccagc cacacggcaa acgcatattc cgacaccgcc
     1861 tccccgctca gcccgcccaa ggcggcactg cccaacaaca gcttgaaaat ccaagccacc
     1921 gccgcgcaag ccgccacgcc cgacagaaac agcgcgtaac gcgaatgccg ccgcatcatc
     1981 tttttgtacg ccagtttcgt caggcccgcc gacacaacca cattcaaaaa cgcaaacaca
     2041 ccgaacacaa accaaacagt gccgtcccaa ccgccacgcc ggccgaatcc gccggccgac
     2101 accgacacca gcagccccgc ccgtccgaac gcacatgcaa aggcatcatg caccgccaac
     2161 gcaaacggca gcgtcaaaaa caccagcccc gcaaacaggg tcgcataagg atatttctcg
     2221 ttcacaaaca tcgccgcccc ccctcttccg aagcaggccg cattataccg cgcaccgcct
     2281 tgccgcccgc ccgaaaagtt gcacaaacaa ccgtttatat atatcataac gaaaaacgcc
     2341 ggtgtagctc agtcggtaga gcagcgcatt cgtaacgcga aggtcggggg ttcgattccc
     2401 ttctccggca ccaataccaa gcacagaccc tccctcggga agcc
//
LOCUS       DAWTCI010000130         2417 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWTCI010000130 DAWTCI010000000
VERSION     DAWTCI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.130"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..759)
                     /locus_tag="WM481_002084"
     CDS             complement(<1..759)
                     /locus_tag="WM481_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFA9081306.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLKM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDSGGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAGTLTAAACPLLQRPFESDGFGLLADEADALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILNSEFARPLTIAEIARRVGLNECYLKR"
     gene            995..1966
                     /locus_tag="WM481_002085"
     CDS             995..1966
                     /locus_tag="WM481_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFA9081307.1"
                     /translation="MKPRFYWAACAVLPAACSPEPAAEKTVSAASQAASTPVATLTVP
                     TARGDAVVPKNPERVAVYDWAALDTLTEPGVNVGATTAPVRVDYLQPAFDKAATVGTL
                     FEPDCESLHRHNPQFVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLSR
                     IFGKEARVAELNAQIDALFAQKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGD
                     IGLPPVDESLRNEGHGQPVSFEYIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVC
                     GTNAWKHKQIIVMPAANYIVAGGARQLIQAAEQLKAAFEKAEPVAAQ"
     gene            1999..2322
                     /locus_tag="WM481_002086"
     CDS             1999..2322
                     /locus_tag="WM481_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFA9081308.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGNMTIREGEMAVVPKSVSHRPRSENGCSVVLIDLPD
                     PSEAA"
BASE COUNT          530 a          662 c          751 g          474 t
ORIGIN      
        1 gcgtttcagg tagcattcgt tcagcccgac gcggcgggcg atttcggcga tggtcagcgg
       61 gcgggcgaat tcgctgttca aaatatcggc ggcttcgtct atgcgccggc ggcggtagcc
      121 gttgtcgtgg cggcggaagg tgaagcgtaa taatcgggcg gagagttcca gcgcgtcggc
      181 ttcgtcggcg agcaggccga agccgtccga ttcgaacggg cgttgcagca gggggcaggc
      241 cgccgccgtc agtgtccccg cgttttgcgc cagccgttgc agggcgaatc ggcctatcgt
      301 ttgcggcgaa aacaggcgtt cgtccagtaa accttcgtcg tgccagcggc gcagtttttc
      361 cagcgaaaaa tccaaatgca gcgcgcacat gccgccgctg tctggcagca gggtttcgga
      421 tacgtccgcc agttcgccgc gtaccagcca gatttcgccg gcagacgggc ggtattccct
      481 gccgcccatt tgtaaccggt tctgccccga taccatgacg aacaaggcgc agttgtggct
      541 gaaattgtgg atttcggtgg gaaacgcacc cgttccgccg ccgcgcatcc gtgataaggt
      601 gatgcccgag tcgaagcggt tgatgcacat tttcagatgc aaaccgggct gttttgcctg
      661 cgcaatgagt gcgctgtcgg aacagccgtc caacgcccag ccggatttat cggagcggac
      721 ataggtttgg tactggcggt agatggcggc ggtgttcatg attggatagg aacgagttgt
      781 ctaacaaatg aattaaatag gaattattac caataatcaa gcgcagggat tggttggaac
      841 ggaaaaggtc gtctgaaagg gtgtttcaga cgaccttttc cgtttcggga atttgttttg
      901 ccgtatcggg aattttgcgt tttgcggcgt ggtttctgca ggttgtttgc ttaataataa
      961 acattcttat tcgtatgcaa aggaaccgca caccgtgaaa ccgcgttttt attgggcagc
     1021 ctgcgccgtc ctgccggccg cctgttcgcc cgaacctgcc gccgaaaaaa ctgtatccgc
     1081 cgcatcccaa gccgcatcca cacctgtcgc cacgctgacc gtgccgaccg cgcggggcga
     1141 tgccgttgtg ccgaagaatc ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac
     1201 gctgaccgag ccgggcgtga atgtgggcgc aaccaccgcg ccggtgcgcg tggactattt
     1261 gcagcctgca tttgacaagg cggcaacggt ggggacgctg tttgagcccg attgcgaatc
     1321 cctgcaccgc cacaatccgc agtttgtcat taccggcggg ccgggtgcgg aagcgtatga
     1381 acagttggcg aaaaacgcga ccaccataga tttgacggtg gacaacggca atatccgcac
     1441 cagcggcgag aagcagatgg agaccctgtc gcggattttc ggtaaggaag cgcgcgtggc
     1501 ggaattgaat gcgcagattg acgcgctgtt cgcccaaaag cgcgaagccg ccaaaggcaa
     1561 aggacgcggg ctggtgctgt cggttacagg caacaaggtg tccgccttcg gcacgcaatc
     1621 gcggttggca agttggatac acggcgacat cggcctgccg cccgtggacg aatctttacg
     1681 caacgagggg cacgggcagc ccgtttcctt cgaatacatc aaagagaaaa accccggctg
     1741 gattttcatc atcgaccgca ccgccgccat cgggcaggaa gggccggctg ccgtggaagt
     1801 gttggataac gcgctggtat gcggcacgaa cgcttggaag cacaagcaaa tcatcgtcat
     1861 gcctgccgcg aactacattg tcgcgggcgg cgcgcggcag ttgatacagg cggcggaaca
     1921 gttgaaggcg gcgtttgaaa aggcagaacc cgttgcggcg cagtagtttt attcaaaaca
     1981 aaccacaagg agaaccccat gcaaaacgaa accatcaacc tgaaacagca ccttgccgcc
     2041 atcaaagaat actggcagcc cgaaatcatc aaccgccacg ggttccaatt ccgcctggtc
     2101 aaacttttgg gcgattacgg ctggcacacg cacggataca gcgacaaagt gctgtttgcc
     2161 gtggagggcg acatggcggt ggacttcgcc gacggcggca acatgacgat acgcgagggc
     2221 gagatggcgg tcgtgccgaa gtcggtgtcg caccgcccgc gttcggaaaa cggctgctcg
     2281 gtggtgctga ttgatctgcc cgacccgtcc gaggccgcct gaaaacgaag tttccgaagg
     2341 aagctgagtt tctgcgaagc taaaagcagc ctacaccttc aatatgccaa aagcatagcg
     2401 gattaacaaa aatcagg
//
LOCUS       DAWTCI010000131         2405 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWTCI010000131 DAWTCI010000000
VERSION     DAWTCI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.131"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            179..355
                     /locus_tag="WM481_002087"
     CDS             179..355
                     /locus_tag="WM481_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081309.1"
                     /translation="MNPTKQSKKSYESKRVLKHVSFNTEKEANLLEFSNNLDFSKWVK
                     EKLKHELELEKLKK"
     gene            854..1696
                     /locus_tag="WM481_002088"
     CDS             854..1696
                     /locus_tag="WM481_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081310.1"
                     /translation="MQNTISVFSFKSQNVRTQILGAEPWFCLGDVAEILQIQNARQLP
                     LKDQGIQKSSVATKKGNQELLFINEPNLYRVIFRSRKAEAVKFQDWIFEEVIPQIRKT
                     GGYQITPKTTADDRTGLRRAVAALVGRKRIGYSSAYSMIHQRFNVEAVEGIPADKLPE
                     AVAYVHALTLHTGLAGEVPDREPLPAPQPALPISGNALADIAAMVYYGTRMIELGKDV
                     SAPLKQLGCKQAVTMWTVWHETRSILKRSVAALEVLRGYADKDASGRIAACLEGIYGK
                     AAAR"
     gene            complement(1897..2277)
                     /locus_tag="WM481_002089"
     CDS             complement(1897..2277)
                     /locus_tag="WM481_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFA9081311.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKPV
                     TVVPLSSTEPVPLADCHHKMSENPLPDKPHIQCRAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAVKTAVAKAFKLY"
BASE COUNT          650 a          596 c          561 g          598 t
ORIGIN      
        1 aggtgcaaat aaggtgcaaa taaaattagt ttttcccaat gggttaaaga aaaaatccga
       61 aaagagttct taaaatagcc cgaccgccgc caagccgcaa acttggcggt ttttttgttg
      121 ccctatgatt ttaaatcgag taaaatgtat ctacgattat tttcaacaag ggggaagaat
      181 gaacccaacc aaacaatcca aaaaaagcta cgaatctaag cgcgttttaa agcacgtttc
      241 atttaacact gaaaaagaag ctaatttatt ggaattttcc aataatttag acttctccaa
      301 atgggtaaaa gaaaagctga aacatgaact tgaattagaa aaactgaaaa aataatagaa
      361 atggcttgca tgaatcgtag atacaaatta taatttcaac acttgctgat tgcagataaa
      421 aaagaagccc aaaccaagct gtaacttgat ttgggcaggt ggaaagcaga gcggcaactc
      481 tctttccgcg tctatctacc atgaaataga aaggtcattc taccatgaat actgtccaag
      541 ttttaaactt tcaacaaagc tccgtccgtg cttcccccca aaatattgac gcaatagcgg
      601 aataatagta aagttcggtt cacaggtttg gtcgcctgtt tctttcgtgg ttattgtcgc
      661 gcttgcggct tttgttttga taagagttcc ctgaagggtg tatgttaaac atacaccctt
      721 gctgaaaaag ccaacgggcg gactttcggg acatctccgg atgtgctgtt tccacgagca
      781 gtcgaccaac ctgaaacgtc cgcctacctc ttcttggtcg aagagaggct ttcaaacttt
      841 cgtggagctt aaaatgcaaa acaccatttc tgtattttct ttcaaatctc aaaatgtccg
      901 tactcaaatc ttgggggctg aaccttggtt ttgccttggc gatgttgcgg aaattcttca
      961 aattcaaaat gcccgtcagt tgccattaaa agaccagggc attcaaaaat ccagcgttgc
     1021 aacaaaaaaa ggcaaccaag aattactttt catcaacgag ccgaatctct accgagtcat
     1081 cttccgttca cgcaaagccg aagccgtcaa attccaagac tggatatttg aagaggttat
     1141 ccctcaaatc cgcaaaacag gcggctacca aattactccc aaaaccaccg ccgacgaccg
     1201 taccggattg cgccgggcgg ttgccgcgct tgtcgggcgc aaacgcatag gctactcctc
     1261 cgcgtacagt atgatacacc aacgcttcaa cgtcgaagcc gtcgaaggca tccccgccga
     1321 caaactgccc gaagccgtcg cctacgtcca cgcattgaca ctgcacacgg gtttggccgg
     1381 cgaagtcccc gaccgcgaac cgttgcccgc gccgcaaccc gccctgccca taagcggcaa
     1441 cgctttggcc gacattgccg ctatggttta ttacggcaca cggatgatcg aattgggcaa
     1501 agacgtttcc gcgccgctga agcagctcgg ctgcaaacag gcggttacga tgtggacggt
     1561 ttggcacgaa acccgttcaa tcctgaaaag atccgtcgca gccctcgaag tgttgcgggg
     1621 atatgcggac aaagacgcct ccggccgcat agccgcatgt cttgaaggca tttacggcaa
     1681 ggctgcggca aggtaaacgg aaaatgccgc ctgaaggttc ggacggcatt tttattgaag
     1741 ggatgattgg ggatttgtag gcgtttgcag atacgacaag gggcgcattc cgcgccccat
     1801 cacacgcacg gacggcttgt catcgcctgt cgcccgtgcg cggctgccca tatttcggaa
     1861 cagtcttgca agccccttta agggaacggt tttattctag tacagtttga atgccttggc
     1921 aacggctgtt ttaaccgcct gaaaatcctc ttcactgatt attgggatgc aacggtcgcg
     1981 ccctttgggt ttgtatcggt ctaatcgtgc caatccgact gttgccgtca tatcgcattt
     2041 tgcccgacat tggatgtgcg gcttgtccgg taaggggttt tcactcattt tgtggtggca
     2101 gtccgccaaa gggacaggct ctgtgctgct taagggtacg accgttaccg gtttgccgtt
     2161 gtgcctgttt cgcgctatga cgacgacagg gcgtttcttg accatttccg gttcttcata
     2221 accgcgaaag tcgcacatga taactgaacg ttcccttggt tggaatttta aaggcattag
     2281 ccgctcctga cgatgacagg cggctattat agcagttctt acacaaaaaa ccgctttgtg
     2341 taaggcggtt gcaaaaaaaa gccttccaat aaaaatgccg cctgaacctt tcagagtgcg
     2401 aagcc
//
LOCUS       DAWTCI010000132         2330 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWTCI010000132 DAWTCI010000000
VERSION     DAWTCI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.132"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            83..2245
                     /locus_tag="WM481_002090"
     CDS             83..2245
                     /locus_tag="WM481_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFA9081312.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFAGNNGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPSYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRIATRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSEFSIPIKEKKNGQEVDK
                     PMEQQKKDRADEAIVRSYRLTNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHD
                     GKTVSSSSLNPSFGVIWQPREHWSFSASHNYAGRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFRQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSRSKPRFYDTHPKKLLSANPEFGAQTGRTWTASLAYRFKN
                     PNLEIGWRGRYVQKATGSILAAGQKDRDGKLENVVRQGFGVNDVFANWKPLGKDTLNV
                     NLSVNNVFDKFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          659 a          698 c          543 g          430 t
ORIGIN      
        1 taattattat tattttttct tatcctgcca aaccttaacg gtttggctta acttcccttc
       61 atacactcaa aaggacgaac aaatgaacgc cccgtttttc cgcctcagcc tgctctcgct
      121 cacacttgcc gccggctttg cccacgcggc agaaaataat gccaatgtcg cattggatac
      181 cgttaccgta aaaggcgacc gccaaggcag caaaatccgt accaacatcg ttacgcttca
      241 acaaaaagac gaaagcaccg caaccgatat gcgcgaactc ttaaaagaag agccctccat
      301 cgatttcggc ggcggcaacg gcacgtccca attcctgacg ctgcgcggta tgggtcagaa
      361 ctctgtcgac atcaaggtgg acaacgccta ttccgacagc caaatccttt accaccaagg
      421 cagatttatt gtcgatcccg ctttggttaa agtcgtttcc gtacagaaag gcgcgggttc
      481 cgcctctgcc ggtatcggcg cgaccaacgg cgcgatcatc gccaaaaccg tcgatgccca
      541 agacctgctc aaaggcttgg ataaaaactg gggcgtgcgc ctcaacagcg gctttgccgg
      601 caacaacggc gtaagctacg gcgcaagcgt attcggaaaa gagggcaact tcgacggttt
      661 gttctcttac aaccgcaacg atgaaaaaga ttacgaagcc ggcaaaggtt tccgcaatga
      721 caacggcggc aaaaccgtac cgtacagcgc gctggacaaa cgcagctacc tcgccaaaat
      781 cggaacaacc ttcggcgacg gcgaccaccg catcgtattg agccacatga aagaccaaca
      841 ccggggcatc cgcactgtgc gtgaagagtt tgccgtcagc gaaaaaaatt cacggataac
      901 tattaaacgc caagccccat cctaccgcga aaccactcaa tccaacacca acttggcgta
      961 caccggcaaa gatttgggct ttgtcgaaaa actggatgcc aacgcctatg tgttggaaaa
     1021 gaaacgctat tccgccgatg acaaagataa cggctacgca ggcaatgtaa aaggccccaa
     1081 ccatacccga atcgccactc ggggcatgaa cttcaacttc gacagccgcc ttgccgaaca
     1141 aaccctgttg aaatacggta tcaactaccg ccatcaggaa atcaaaccgc aagcgtttct
     1201 gaattcggaa ttctccatcc cgataaaaga aaagaaaaac ggtcaagaag tcgataaacc
     1261 gatggaacaa caaaagaaag accgtgcaga tgaagccatt gtccgttcct accgcctgac
     1321 caacccgacc aaaaccgata ccggcgcgta tatcgaagcc attcacgaga ttgacggctt
     1381 taccctgacc ggcgggctgc gttacgaccg cttcaaggta aaaacccacg acggcaaaac
     1441 cgtttcaagc agcagcctta acccgagttt cggcgtgatt tggcagccgc gcgaacactg
     1501 gagcttcagc gcgagccaca actacgccgg ccgcagcccg cgcctgtatg acgcgctgca
     1561 aacccacggc aaacgcggca tcatctcgat tgccgacggc accaaagccg aacgcgcgcg
     1621 caataccgaa atcggcttca actacaacga cggcacgttt gccgcaaacg gcagctactt
     1681 ccggcagacc atcaaagacg cgcttgccaa tccgcaaaac cgccacgact ccgtcgccgt
     1741 ccgcgaagcc gtcaacgccg gctacatcaa aaaccacggt tacgaattgg gcgcgtccta
     1801 ccgcaccggc ggtctgaccg ccaaagtcgg cgtaagccgc agcaaaccgc gcttttacga
     1861 tacccatcct aaaaaactgt tgagcgcgaa ccccgagttt ggcgcacaaa ccggccgcac
     1921 ttggacggcc tcccttgcct accgcttcaa aaacccgaat ctggaaatcg gctggcgcgg
     1981 acgctatgtt caaaaagcta cgggttcgat attggcggca ggtcaaaaag accgcgacgg
     2041 caaattggaa aacgttgtac gccaaggttt cggtgtgaac gacgtcttcg ccaactggaa
     2101 accattgggc aaagacacgc tcaatgtcaa tctttcggtt aacaacgtgt tcgacaagtt
     2161 ctactatccg cacagccaac gctggaccaa taccctgccg ggcgtgggac gtgatgtacg
     2221 cttgggcgtg aactacaagt tctaaaacgc acatcccgaa aaaatgccgt ctgaaagcct
     2281 ttcagacggc atctgtcctg ataatttgat atatagtgga ttaacaaaaa
//
LOCUS       DAWTCI010000133         2285 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWTCI010000133 DAWTCI010000000
VERSION     DAWTCI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.133"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..664
                     /locus_tag="WM481_002091"
     CDS             <1..664
                     /locus_tag="WM481_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081313.1"
                     /translation="ESTAPKCNINSPELQNQHSGSEESTAVIRTEDLTEDLVVYTPLP
                     PNAGNGKGGLNADAFVSADAETCGRETGEPTSPKAESDSNGNGGLSGKPKNANVPRRR
                     KTHGVPLQEIADLYNEVLGGRLPSVQVLNDTRKRAIANRWCEMLGTAAPNGKVRFGDK
                     ETGLAWFAGFFRKVAMNPFWMGENQTGFAVGFDWIFKAGNFVKILEWHPPKTNQAARG
                     RA"
     gene            661..2022
                     /locus_tag="WM481_002092"
     CDS             661..2022
                     /locus_tag="WM481_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HFA9081314.1"
                     /translation="MNRIEETEAVQSLASVGAEQNILGGILIEPTAIARCAILTPEKF
                     YQAQHRIIFRALLDMAAANEPIDIITLNDKLEARGEAENAGGLAYLIDLNQNTPSAKN
                     ISRYVGIVNDRFVERGLLKASAAIEKIAVSKDGGTVAEKLSKAADELAAAGKDAVKRE
                     TKTFGQTVEDLIGGLDKRLDGVRFGLPTGLMKLDGMTGGLPDGNLIVIAARPSMGKTV
                     LAENIARFALKQGKAVHFQSYEMSAVELARRGMAAECNIPMQNLKTGNLTQSDYANMP
                     IYVSQAKEWKFDVNCDLLNVDELCFLAKEKKLTTGLDLLVVDHLHIMPRAGRDEVAEL
                     GNISRRLKNLAAELNIPVVLVAQLNRGNTKQADKRPNMADIRGSGAIEQDANIIIMPH
                     RESYYDGNENPSIAELIIAKNRDGETGTVVCGWKGQFMKFEEEPDLAWQAPKHDEYDP
                     YSV"
     gene            2039..2269
                     /locus_tag="WM481_002093"
     CDS             2039..2269
                     /locus_tag="WM481_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081315.1"
                     /translation="MRETCFYCNHADFKTNTGTPVRGFAKCAKARNAEEKATYYPRTN
                     PCAAGAFQTASGAAVAKRTAVLGEYPPAMRRI"
BASE COUNT          601 a          561 c          709 g          414 t
ORIGIN      
        1 ggaatcaaca gccccgaaat gcaatatcaa cagccccgaa cttcaaaatc aacattcggg
       61 gagtgaggaa tcaacagccg ttataagaac agaagattta acagaagatt tagtagtata
      121 taccccctta cccccaaacg ccggaaacgg caaaggcggt ttgaacgctg acgcgtttgt
      181 ttccgctgac gcggaaacgt gcgggcggga aaccggcgaa ccgacttcgc cgaaggccga
      241 aagcgacagt aacggcaacg gcggcctttc gggaaaaccg aaaaatgcga atgttccgcg
      301 ccgccgcaaa acccacggcg taccgcttca ggaaatcgcc gatttgtaca acgaagtttt
      361 gggcggccgg ttgccaagcg tccaagtgct gaacgacacg cgcaaacggg cgattgcaaa
      421 ccgctggtgc gagatgctgg gaacggcggc gccaaacggc aaggtgaggt tcggggacaa
      481 ggaaaccggt ttggcctggt ttgccggttt cttccggaaa gtggcgatga acccgttttg
      541 gatgggcgaa aaccaaacag ggtttgcggt cggcttcgat tggattttca aggcgggcaa
      601 tttcgtcaaa atccttgaat ggcatccgcc taaaacgaac caggcggcaa ggggaagggc
      661 atgaaccgaa tcgaggaaac ggaagcggtc caatcactgg ccagcgtagg ggcggaacag
      721 aacattttgg gcggcatctt gattgaaccg acggcgattg cgcggtgcgc aatcctgacc
      781 cctgaaaagt tttaccaggc gcaacacagg attattttcc gcgctctgct ggatatggcg
      841 gcggcaaacg agcctatcga catcatcacg ctgaacgaca agctggaagc gcgcggcgag
      901 gcggaaaacg cgggtggcct ggcttacttg atagacctga accaaaacac cccaagcgcg
      961 aagaatatca gccggtacgt tgggattgtg aacgacaggt ttgtcgagcg cggcttgctg
     1021 aaggcttcgg cggcgattga aaaaatcgcg gtttccaaag acggcgggac ggtcgcagaa
     1081 aagctgtcta aggcggccga cgaattggcg gcagccggca aagacgcggt aaagcgtgaa
     1141 accaagacat tcggccagac cgttgaggat ttgattggcg gtttggacaa aaggcttgac
     1201 ggcgtgcgtt tcggattgcc taccggcttg atgaagcttg acgggatgac cggcggtttg
     1261 ccggatggaa acctgattgt gattgcggcg cgtccgtcta tgggtaaaac ggttttggcg
     1321 gaaaacattg cgcgattcgc gctgaagcag ggcaaggcag ttcatttcca aagctacgaa
     1381 atgagcgcgg tagagctggc aaggcgcggc atggcggctg agtgcaatat ccccatgcag
     1441 aacctgaaaa ccggcaatct gacgcaaagc gattacgcca atatgccgat ttacgtcagc
     1501 caagcgaaag agtggaagtt tgacgtgaac tgcgacctgc tcaacgttga cgagctttgc
     1561 tttttggcta aggagaaaaa actcactacc ggcttggatt tgttggttgt cgatcacctt
     1621 cacatcatgc caagggcagg gagggacgag gtggcggagt tggggaatat atcgcgccgt
     1681 ttgaaaaact tggcggcaga gctgaatatc cccgtcgtct tggttgccca gttgaacagg
     1741 ggaaacacaa agcaggcaga caaacgcccg aacatggcag acattcgcgg cagcggcgcg
     1801 attgagcaag acgcaaacat catcatcatg ccgcaccgcg aaagctacta cgacggaaac
     1861 gagaatccga gcattgccga gctgattatc gccaagaacc gggacggcga aacgggaacg
     1921 gtggtttgcg gctggaaagg gcaatttatg aagttcgagg aagagcctga tttggcatgg
     1981 caagccccca aacatgatga atatgaccct tacagtgtct agtgcgggag gccggtaaat
     2041 gcgtgaaacc tgtttctatt gcaaccatgc cgacttcaaa accaacaccg gcacgccggt
     2101 gcgcggtttt gcgaaatgcg cgaaggcgcg gaatgcggag gaaaaagcga cgtactaccc
     2161 gcgaaccaat ccctgcgccg ccggggcgtt tcagacggca tcgggggcgg cagtcgcaaa
     2221 aaggacggcg gtgcttgggg aatatccccc cgcaatgcgc cggatttgag cgggaaggcg
     2281 ggtaa
//
LOCUS       DAWTCI010000134         2269 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWTCI010000134 DAWTCI010000000
VERSION     DAWTCI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.134"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(158..468)
                     /locus_tag="WM481_002094"
                     /pseudo
     CDS             complement(158..468)
                     /locus_tag="WM481_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            355..867
                     /locus_tag="WM481_002095"
     CDS             355..867
                     /locus_tag="WM481_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFA9081316.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLIVLPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            947..2026
                     /gene="aroB"
                     /locus_tag="WM481_002096"
     CDS             947..2026
                     /gene="aroB"
                     /locus_tag="WM481_002096"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFA9081317.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFIQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMALERAPLTQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAVEAEMGYGVWLHGEAVAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLAHMSHDKKVSGGIMRFIGL
                     NRLGEAVITEITDTDILRRTLQPYL"
BASE COUNT          553 a          706 c          572 g          438 t
ORIGIN      
        1 tccctttgag ctaaggcggg gcaacgccgt accggttttt gttaatccgc tatagtattg
       61 ataaacatat tatcttcaat atattcaatt ggataattgt ttacctaagc aaagataatt
      121 gccttttcct gacaaattaa gtgaaatcaa cggattgtca aaacacagcc tgaaataaaa
      181 aacctccctg atttctttta tttttcctta aaatcagaaa agttcgggat ggtcgggtta
      241 tttttccaaa cgtaccgccg ccctgccgat ttcgtataaa atcccgccgt aacccgacaa
      301 gcccgaaccc tgccgccccg aaaggcgggg ggggtcaaac attaaggaac cgtgatgaaa
      361 aactttaacg gcaaactcat cctcatcgga ctgatgggcg cgggcaaaac tacgctgggc
      421 cggcaaatgg cgcagcggct ggattaccgt ttttacgaca gcgatcacga aatcgccgca
      481 gcggcgggcg ttcccatccc caccatattt gaaatggaag gcgaacaggg attccgttcg
      541 cgcgaaaccg ccatactcaa aaaactgatt gtcctgccgc atatcgtcct gtccaccggc
      601 ggcggcgcgg tgttaaaaga agaaaaccgc gcccttatcc gcaaaagcgg cacggtcgtt
      661 tatctgcacg ccccgcccga aaccctgctc gaacgcacgc gctgcgacaa cagccgccct
      721 ttgctgcaag ttgccgatcc tttggcgaaa ttgcgcgaac tctacgccgc acgcgacccc
      781 gtttaccgcc aaaccgccga ctttaccgta gaatcggcaa actgccggga aaccgtgcaa
      841 accctgctca aacgcttatc ccgataaacc ggcatatgcg ccgctccccg aaaaccaaac
      901 cgcaccccgc ccggcgggcc ggcggttcaa actttaagga acaacaatga aaacactgac
      961 cgtacacacg ccttcgcaca gctaccccat ctttatcggc aacgggctgc tgccgcaggc
     1021 gggaagcctg ctcaaaccgc atttgggcaa acgcgccgcc atcatcacca acgaaaccgt
     1081 cgccccgctc tacctcggca cgcttcagac ggcattggat gcggcaggcg tatcccattt
     1141 cagcatcatc ctgcccgacg gcgaggcgca caaaaactgg cagacgctca acctcatctt
     1201 tgacgggctg atgcaaaacc gcgccgaacg taaaaccacg ctgattgcgc tgggcggcgg
     1261 cgtgatcggc gacatggtcg gctttgccgc cgccacctac cagcgcggcg cgccgttcat
     1321 ccaaataccg accacgctgt tgagtcaggt cgactcatcg gtgggcggaa aaaccgccat
     1381 caaccacccg ctcggcaaaa atatgatcgg cgcgttttac cagccgcagg cggtgcttgc
     1441 agatttggac acgctgcaca ccctgcccgc ccgcgaactg tccgccggta tggcggaagt
     1501 catcaaatac ggcgcgctcg gcgacatcgg cttttttgaa tggctggaac agcatatgcc
     1561 cgaactgatg gcactcgaac gcgcgcccct cacccaagcc gtgtaccgct gctgccaaat
     1621 gaaggcagac atcgtcgccc aagacgaaac cgaacagggc atacgcgcat ggctcaacct
     1681 cggacacacc ttcggacacg ccgtcgaagc ggaaatgggt tacggcgtat ggctgcacgg
     1741 cgaggccgtt gccgccggct gcgtgttggc ggcgcgtttg tccgaacaac tgggcaaaac
     1801 ctccgccgca gataccgcgc ggctcgccgc cctgctcgaa gccgccggac tgccgtccgc
     1861 accgcccgtg tttgcctttg aaaaatggct ggcacatatg agccacgaca aaaaagtcag
     1921 cggcggcatc atgcgcttta tcggtctgaa ccggctgggc gaagccgtca tcaccgaaat
     1981 taccgacacg gacatcctcc ggcgcaccct gcaaccgtat ctctgatttc ctctgccgat
     2041 gtgttgccgc gcgggtttga cgcacgatga tgactttcca tcatcattct ccgcgaaagc
     2101 gggcatccgg ttattccata ttgcagggat ttatccggaa taacgacaac ctgtccgccg
     2161 tcatttccgc gcagatggaa atttggaaat tcaaaatcac aggaatttat ccggaattat
     2221 agcggattaa caaaaatcag gacaaggcgg cgaagccgca ggcagtacg
//
LOCUS       DAWTCI010000135         2265 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWTCI010000135 DAWTCI010000000
VERSION     DAWTCI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.135"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            112..336
                     /locus_tag="WM481_002097"
     CDS             112..336
                     /locus_tag="WM481_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081318.1"
                     /translation="MFIFYTVNPEHVYFPKAYIMKVFKDKGYESQCITTVSFYICNPT
                     LKQKTENEAYEYGRLFVKELMHKECNRESL"
     gene            323..1651
                     /locus_tag="WM481_002098"
     CDS             323..1651
                     /locus_tag="WM481_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFA9081319.1"
                     /translation="MKAFEDKALAVSADMADALADGGAAATAVGCPPRLIGGEQNKTP
                     NPKGAEKTENQEFQFEYFSHFVSDGKGRFIEIPLRRGRDDGAFIDQITFTIHENSMTK
                     VTGKGLVSDTEFVVRYSELLEEILGFGITKKLPFKGKFFYQSCYQFGPDNVEYGKVHY
                     GGQCETMLVELNGTGCMAALPGWENRLYEFLSKCVRPKITRIDVAHDFFNGEYTPDQA
                     MLDHDNGHYDVHNMRPKSECRGTAWRNEDGSGKTFYIGKRENSKFTRVYEKGRQFGDV
                     DSPWVRFEGDIEIPLDVLLYSGSYLGGAYPICKEIFKTEAKRMEVKVKNVNLIFDVKL
                     FHARNQVGKMVNFLRDIGWDDSRIVDELVKGVEGYPKGLQPEQYDCKNQTQKFQYIHE
                     EQKAINALNIETLFDDLIEEREYAFPQDRERQHIKDIEYEERQISDFLNN"
     gene            1665..1976
                     /locus_tag="WM481_002099"
     CDS             1665..1976
                     /locus_tag="WM481_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081320.1"
                     /translation="MFETSQVTTYTATLLGAKKFKGEIDGNKIDSCTVLVASPMPSNG
                     NAVGFTSESMKFGDSHNFEKLKNLKFPCAVDLTVAMESTGKGLVQKLLDFQVKGAAPK
                     A"
     gene            1990..2193
                     /locus_tag="WM481_002100"
     CDS             1990..2193
                     /locus_tag="WM481_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081321.1"
                     /translation="MSRYQQKFIVQELENHEFIYPDSFGDIGFTSNIKSAGKYDSYED
                     AFSSALEEIGGEFVIFGFYEKED"
BASE COUNT          736 a          444 c          534 g          551 t
ORIGIN      
        1 ggagcggata aattctcgca atctaaaacc ttatatattg cgctccataa ggggcgcata
       61 tttttaatcc ttgtaaagcc ccttaactct agtcagtgaa ggggcttaat catgtttatt
      121 ttctatactg tcaatcccga acacgtatat ttccccaaag catacatcat gaaagtgttc
      181 aaggataaag gctacgaatc ccaatgcatc acaacagttt ctttttatat ctgcaatccg
      241 actttgaagc aaaagaccga aaacgaagcg tatgaatacg gcagattgtt tgttaaagaa
      301 ctgatgcata aggaatgcaa ccgtgaaagc ctttgaagat aaagccttgg cggtgtccgc
      361 ggacatggcg gacgcgttag cggacggcgg ggctgcggca accgccgtag gctgcccccc
      421 taggctaata gggggggagc aaaataaaac cccaaatccg aagggtgcgg aaaaaacaga
      481 aaaccaagaa tttcaattcg agtatttcag tcattttgta tcggacggca aaggcagatt
      541 tatagaaata ccgttaaggc gtggcaggga tgacggagcg ttcatagacc aaatcacttt
      601 cacgatacat gaaaattcca tgacgaaagt aaccggaaag ggattggtat cagatacgga
      661 attcgttgtc cggtatagcg agctgcttga agaaatcttg ggattcggca ttaccaaaaa
      721 attaccgttt aaaggaaagt ttttctatca aagctgttat caattcgggc ccgataacgt
      781 cgaatacgga aaggttcatt acggcggcca atgtgaaacg atgttggttg aattgaacgg
      841 tacaggctgc atggctgctt tacccggttg ggaaaaccga ttgtatgaat ttttaagtaa
      901 atgcgtccga cccaaaataa cccgtatcga tgtcgcccat gattttttca acggagagta
      961 cacacccgat caggcaatgt tagaccacga taacggacat tacgacgtac acaatatgcg
     1021 gccaaaaagc gaatgtcgcg gcacggcatg gcgcaatgaa gacggcagtg gaaaaacttt
     1081 ttacatcggc aagcgcgaaa attcaaaatt tacccgtgtt tacgagaaag gcagacagtt
     1141 tggagatgtt gatagccctt gggtcaggtt tgagggggat atagaaatac ccttggatgt
     1201 cctactttat tcggggtcgt atctaggcgg cgcatatccg atttgcaagg agatttttaa
     1261 gacagaagcc aagcgcatgg aagtaaaagt taaaaacgtt aatctgatat tcgacgttaa
     1321 gctttttcat gcaaggaatc aagtcggcaa gatggttaat ttcctccgcg atatagggtg
     1381 ggacgatagc aggatagttg atgagctggt gaaaggcgtt gaaggttatc caaaaggttt
     1441 acagcctgag caatacgatt gcaaaaacca aacgcagaaa ttccagtata tccacgaaga
     1501 acagaaagcc attaacgcat tgaacattga aacactgttt gatgatttga ttgaagagag
     1561 agaatacgca tttcctcaag acagggaacg gcaacacatc aaggatattg agtacgagga
     1621 acgtcaaata tccgattttt taaacaactg aaggaaattg aaaaatgttt gaaacaagcc
     1681 aagtaaccac atataccgcc actttgttgg gcgcaaaaaa attcaaaggc gaaatcgacg
     1741 gtaacaaaat cgattcatgc accgtcttgg tagccagccc catgccgtca aacggcaatg
     1801 ccgtgggctt tacctcggaa agcatgaagt ttggtgacag ccataatttt gaaaaattga
     1861 aaaaccttaa attcccctgt gcggttgatc taaccgttgc catggaatca acaggaaaag
     1921 gcttagtgca gaaattgttg gatttccaag taaagggcgc agcacctaaa gcctaagaaa
     1981 ggctgaatca tgagcagata ccaacagaaa tttatcgtac aggaattgga aaatcacgaa
     2041 ttcatctatc ccgattcgtt tggcgatatt ggatttacgt cgaacatcaa atcagccggt
     2101 aaatatgaca gctatgaaga cgcttttagc tcggcattgg aagagatagg cggggaattt
     2161 gtaattttcg gattctacga aaaagaagat taagtttaag aggctcggca ggcggtctct
     2221 aaaaccttca caaagcccgc aaacacattt ttttaaaaat ttcgt
//
LOCUS       DAWTCI010000136         2014 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWTCI010000136 DAWTCI010000000
VERSION     DAWTCI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.136"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            442..1221
                     /locus_tag="WM481_002101"
     CDS             442..1221
                     /locus_tag="WM481_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFA9081322.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGKGFIWRAYSVVSAEYADTLEYFAVLIQDGPMSALFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKLTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            1430..>2014
                     /locus_tag="WM481_002102"
     CDS             1430..>2014
                     /locus_tag="WM481_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HFA9081323.1"
                     /translation="MAAHNKLTQKQIEAAKADGKQSKLADGGGLYLLLHPNGSKYWRM
                     RYRHGGCEKTLALGVYPAVSLKQARELARAARAQTAAGIDPVAEHHRTRPGSGRSLPE
                     IARAWYGSRQGQRADNTLEGDRRSLAYLTDYYKDNTDINDITTAGVSKFIEHLNRINI
                     PACARRTVQILAQIWDYAVQRGIITDERRNPATTA"
BASE COUNT          614 a          601 c          398 g          401 t
ORIGIN      
        1 catgtaaaac tgtatataat caattcagat ttccaaaaaa ttgtttttta tgataaaggt
       61 aagatagttt ttgaggaatt aatagaacta gataggaaag ggaaagtttt acctcaatat
      121 ctatttgatt catatttgaa aaatgtttaa tattacgaat gtgattacaa gaatggcaag
      181 atgcaacttt taaaaaagaa aaattacatt cttttgatag atatttttat tattataaac
      241 ctataaactg taggccgaaa cttttgtaaa actgtccata ctcattaccg cctagtcggt
      301 aatgcatttt ttagtagtat ttgaaaaagt atgatgataa aaacggttaa gcctaacttt
      361 ttcataaaat ccagcagagt aaggcagaaa aaaacaccca aattggctat aatcccgaca
      421 aacacactca aggacaacaa catggcagcc tcgcccgaag caaaattcac cgaagaaaag
      481 attttgtggg tcaaacacca cacgccgaaa ctcatcactt tcgccatcag ccgtcccgaa
      541 tcctaccgtt ttaaagccgg acagttctcc cgactcggtt tctacgaagg taaaggcttt
      601 atttggcgtg cctattccgt cgtttccgca gaatatgccg acacgctcga atattttgcc
      661 gtactcatcc aagacggccc catgtcggcc cttttcgcca aaatgcaaca gggcgacacc
      721 atcctgctcg ataaaaatgc cactggcttt ctcctgcccg aacgcttccc cgacggcaag
      781 gatttggtga tgctctgtac cggctcgggc atcgccccct tcctttccat tctcgaacaa
      841 cccgaaatcc ggcagcgttt cgataccgtc aacctgatac attccgtatc ttttcccgaa
      901 gaattgattt tcaacgaccg gctggccgca ttgtccgaac atcccctggt aggcgaatac
      961 ggacactcgt tccgtttcgt ccctgttacc acccgtgccg ccaacccctc gggcttaagc
     1021 ggaaaacgca ttccggaact cttaaaaaac aacagcatcg aacaggcgct gcataccaag
     1081 cttaccccgg aatccacacg gtttatgatt tgcggtaacc cggaaatggt caaagacact
     1141 ttccaaacgc tgctcgatat gggttacgcc atgcaccgca accgcattcc cggtcaaatc
     1201 atgatggaaa acggcttcta aaaacgaacg ctcccaaccc ctacccacgc ccacacacaa
     1261 aacaaaataa aaaacagcga tatacaaaaa ccaatttgca aaaacagacc tcacgcctat
     1321 actgccactc acagcaagca acacaaaaaa gcgggtacaa attagggtac aaaacacaag
     1381 cccgaacaca gaagcacccc aaccaaaccc aaaggcaaag gcaaacaaaa tggcggcaca
     1441 caacaaactg acccaaaaac aaatagaagc agccaaggca gacggcaaac aaagcaagct
     1501 ggccgacggc ggcggcttat atctgctgct gcaccccaac ggcagcaaat actggcggat
     1561 gcgctaccgg cacggcggat gcgaaaaaac cctagccttg ggcgtatatc ccgccgtcag
     1621 cctcaagcag gcccgcgaac ttgcccgcgc cgcccgggcg cagaccgccg ccggcatcga
     1681 ccccgtcgcc gaacaccacc gcacccgtcc cggcagcggg cgcagccttc ccgaaatcgc
     1741 ccgcgcgtgg tacggcagcc gtcaagggca acgcgccgac aacacactgg aaggcgaccg
     1801 ccgttcactc gcctacctga ccgactacta caaagacaac accgacatca acgacatcac
     1861 caccgccggc gtatcaaaat ttatcgaaca cctcaaccgc atcaacatcc ccgcctgcgc
     1921 ccggcgcacc gtccaaatcc tcgcccaaat atgggactat gccgtccagc gcggcatcat
     1981 taccgacgaa cggcgcaacc ccgcaaccac cgcc
//
LOCUS       DAWTCI010000137         1975 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWTCI010000137 DAWTCI010000000
VERSION     DAWTCI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.137"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            141..368
                     /locus_tag="WM481_002103"
     CDS             141..368
                     /locus_tag="WM481_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9081324.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPRMKAGGRGKPVTHYSIVKNGAEV"
     gene            365..1342
                     /locus_tag="WM481_002104"
     CDS             365..1342
                     /locus_tag="WM481_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9081325.1"
                     /translation="MSARLMGMAFKTGIPRGQRFVLVKLCDCANDEGLCYPSQETLAE
                     DTGFAETAVRQHIKWLKDNNFIKSARRQRGRERKSDIYRINVALLEKCYAEAAKRKAA
                     RQAKMWEEPLDYEPSDYEPSDFDAKNHQILSGEPSDFALRTVRFCAKNHQILSGEPSD
                     FDGSLYVEPSVEPSVEPSVEPSGSNARGARAPAGPHPAKPQTAPPETAPAAKAKKTGR
                     HETELSLLADYGITGQVAADFLQVRKAKRQPLTETAMRLIAADAEKCGMTALQAAEYA
                     IASGWGSFRADWLQNKTFGRSGNRGGPTHNQTAAVPDAGSYGDMPTTDF"
     gene            1359..>1975
                     /locus_tag="WM481_002105"
     CDS             1359..>1975
                     /locus_tag="WM481_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFA9081326.1"
                     /translation="MALRNASDFLGAYGGGVRVERRQCAEHGGYAAKSVLRGVWTGCP
                     ACRKLEAADEMAAYAETLRRGAMRDALEKRIGRSGIAPRFRNCRIENYAVSDSIPGMA
                     RAKAAAAEYAANFADVLQTGRSMIFSGRRGTGKNHLACGIAREVIAAGKSALVITVGD
                     MLRTVKDSFGGGGGEAGAVGVFVKPDLLVLDEFGAGSLSETDGRI"
BASE COUNT          452 a          517 c          684 g          322 t
ORIGIN      
        1 cggttgcaac ggggtgacgg caaatcggaa agacggctga ccgcccggac agacgggcgg
       61 ccgataaaga aaaacccgca cggggcgggt aatccccctg aattgcaggg aagcggttca
      121 ggtaacggcg aaaggcgatt atgaatcaaa aacaaacgca atgcaaacaa attgtcgatt
      181 acatccgtaa caagggatgc atcacatccc ttgaggctta tcagaacctg aaggtgacgc
      241 agcttgcggc acggataacc gacttggaag gcaggggctt cgtgtttgcc aagccgcgca
      301 tgaaggcggg cggccgcggg aagcctgtta cgcattattc gattgtcaaa aacggagcgg
      361 aagtatgagt gcgaggctga tggggatggc tttcaaaacg ggtatcccga gggggcagcg
      421 ttttgttttg gtgaagttgt gcgactgcgc caacgacgag ggcttgtgtt atccgtcgca
      481 agaaacgctg gcggaagata cgggcttcgc cgaaaccgcc gtacggcagc atatcaagtg
      541 gctgaaggat aacaatttca tcaagtccgc ccggcggcag agggggcggg agaggaagtc
      601 cgacatctac cgcatcaacg tcgccctgct tgaaaaatgc tatgcggagg cggcaaaacg
      661 gaaggcggcg cggcaggcaa aaatgtggga agaaccattg gattacgaac cgtcggatta
      721 cgaaccgtcg gattttgacg ctaagaacca tcagattttg agcggcgaac cgtcagattt
      781 tgcgctaaga accgtcagat tttgcgctaa gaaccatcag attttgagcg gtgaaccatc
      841 agattttgac ggttccttat atgtagaacc gtcagtagaa ccgtcagtag aaccgtcagt
      901 agaaccgtca ggatcaaatg cgcgcggcgc gcgcgcccct gccggaccgc accctgcgaa
      961 accgcaaacg gcgcctcccg aaaccgcacc ggcggcgaag gcgaaaaaaa ccggcaggca
     1021 cgaaaccgag ctttcgctgc ttgccgacta cggcatcacg gggcaggtgg cggcggactt
     1081 cctgcaagtc cgcaaggcaa aacggcagcc gctgacggaa acggcaatgc gcctgattgc
     1141 cgccgatgcg gagaaatgcg ggatgacggc gctgcaggcg gcggagtacg ccatcgccag
     1201 cggctggggc agcttccgcg ccgactggct gcaaaacaaa actttcggca ggtccggaaa
     1261 ccgcggcggc ccgacgcaca accaaaccgc cgccgtgccg gatgcgggaa gctacggcga
     1321 tatgccgacg acggattttt gagggggggg gttcggatat ggctttgagg aacgcgtctg
     1381 atttcttggg ggcttacggc ggcggcgtgc gggtcgagcg gaggcaatgc gcggaacacg
     1441 gcggatacgc ggcgaaaagc gttttgcgcg gcgtgtggac gggctgcccg gcctgccgga
     1501 agctggaggc ggcggacgaa atggcggcat acgcggaaac gctgcgccgc ggggcgatgc
     1561 gcgacgcgct ggaaaaacgc atcgggcgtt cgggcatcgc cccgcggttc agaaactgcc
     1621 ggattgaaaa ctacgccgtc agcgattcga tcccggggat ggccagggcg aaggcggccg
     1681 ccgccgagta tgcggcaaac ttcgccgatg tgttgcagac ggggcggagc atgatttttt
     1741 cgggcaggag gggcacgggc aaaaaccacc ttgcctgcgg catcgcccgc gaagtcatcg
     1801 ccgccggcaa aagcgcgctg gtcatcacgg tgggcgatat gctgcggacg gtcaaggaca
     1861 gtttcggcgg cggcggcggc gaggcggggg cggtcggggt tttcgtgaag cccgatttgc
     1921 tggtgctgga cgagttcggc gcgggcagtc tgtcggaaac ggacgggcgg atttt
//
LOCUS       DAWTCI010000138         1810 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWTCI010000138 DAWTCI010000000
VERSION     DAWTCI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.138"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            38..364
                     /locus_tag="WM481_002106"
     CDS             38..364
                     /locus_tag="WM481_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081327.1"
                     /translation="MTRSPFTGAGQVRGNTRTAIADNTAISKPVIPAQAGIRICRDSL
                     KSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMELKHCKEMLEQKDKEIELLR
                     KLTETV"
     gene            453..1022
                     /gene="rsmD"
                     /locus_tag="WM481_002107"
     CDS             453..1022
                     /gene="rsmD"
                     /locus_tag="WM481_002107"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFA9081328.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFASADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGMEAASRNAKRVVIADNNRQTVQTLEKNSRELGLG
                     QVQTVCSDGIAYLANLKEKFDVVFLDPPFAWQSWESLFNVLGTRLNDGAYVYIEAGRQ
                     PDKPDWLTGYREGKSGQGTFELRVFQVAE"
     gene            1145..1396
                     /locus_tag="WM481_002108"
     CDS             1145..1396
                     /locus_tag="WM481_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFA9081329.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            1493..1576
                     /locus_tag="WM481_002109"
     tRNA            1493..1576
                     /locus_tag="WM481_002109"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1527..1529,aa:Tyr,seq:gta)
     gene            1607..1680
                     /locus_tag="WM481_002110"
     tRNA            1607..1680
                     /locus_tag="WM481_002110"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1639..1641,aa:Gly,seq:tcc)
     gene            1690..1764
                     /locus_tag="WM481_002111"
     tRNA            1690..1764
                     /locus_tag="WM481_002111"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1722..1724,aa:Thr,seq:ggt)
BASE COUNT          498 a          413 c          476 g          423 t
ORIGIN      
        1 ccgacactga tgccgtctga accttcagac ggcatttttg acgcgctcgc cgtttacggg
       61 cgcggggcag gtgcggggaa atacccgtac cgccattgcc gacaacaccg caatctcgaa
      121 acccgtcatt cccgcgcagg cgggaatccg gatttgtcgg gattcgctca aatcgggcgg
      181 cggcgggatg gtgttgcaga ttaacgatgt ggataccaac agcggggaat ttgcaatcta
      241 taccgctcag gatgcatcgg taaagctgga tttgttaaaa atggaattaa aacactgcaa
      301 agaaatgttg gaacaaaaag acaaagaaat cgagttgctc cgcaagctga ccgaaaccgt
      361 ttaaacagat atgccgtctg aaaaaagttt tcaggcggca tattcttcga caggtcttgt
      421 ataataccgt ttgaactttc aggcttttga ttatggcggc aggcaaacat accaaacaca
      481 gcaaccgggt acgcattatc ggcgggcaat gccggggcag gaaattgagt tttgcatccg
      541 ccgacggact gcgcccaacc cccgacagcg tgcgtgaaaa gctgtttaac tggctgggac
      601 aggatttgac gggaaaaacg gttttggatc tcttcggagg cagcggcgca ctcggcatgg
      661 aggcggcatc gcgtaatgca aaacgggtcg tgattgcaga caacaaccgt caaacggtgc
      721 aaacccttga gaaaaacagc cgcgaactgg gtttggggca ggtgcaaacc gtctgttcgg
      781 acggcatcgc atatttggca aacctaaaag agaaattcga tgtcgtcttc cttgatccgc
      841 cgtttgcgtg gcaaagttgg gaaagtcttt tcaatgtatt gggcacacgc ctgaatgacg
      901 gcgcatatgt ttatatcgag gcaggcaggc agccggacaa acctgattgg ctgacgggat
      961 atagggaggg gaagtcgggg cagggtacat ttgaattaag ggttttccaa gtggctgaat
     1021 aatatgcgct ttgataatca tttccgggtt gtaaacattc gtttgcaacc gtccggttca
     1081 aaaaaacctt gtgctataat ccgcgcccgc ccggttttga taatttagtg gaaaaggaaa
     1141 agaaatgtcg ctttttatta ccgacgagtg catcaactgc gacgtatgcg aacccgaatg
     1201 ccccaatgat gccatttccc aaggcgagga aatttacgaa atcaacccca acctctgcac
     1261 gcagtgcgtc ggacactacg atgagccgca gtgccagcag gtttgcccgg tggactgcat
     1321 cctgattgac gaagaacatc ccgaaaccca tgacgagttg atggcgaaat acgaaaagat
     1381 tatccagttt aaataaattc tttttaaaac atcaaattat gtctgttttg aaataaaatc
     1441 aaaaaaaaac ttgacggaaa agcaagccgc taataaacta gtgttctctt ttggagggat
     1501 tcccgagcgg tcaaaggggg cagactgtaa atctgttgcg aaagcttcga aggttcgaat
     1561 ccttctccct ccaccaaaat tcttacttgg ggcagtagcg agtaatgcgg gtgtagctca
     1621 atggtagagc agaagccttc caagcttacg gtgagggttc gattcccttc acccgctcca
     1681 aacaattagg cccatgtagc tcaggggtag agcactccct tggtaaggga gaggtcggca
     1741 gttcaaatct gcccatgggc accatctctc gattattcat ttctttaagg cttagatata
     1801 taggatattg
//
LOCUS       DAWTCI010000139         1698 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWTCI010000139 DAWTCI010000000
VERSION     DAWTCI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.139"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(126..1094)
                     /gene="ispH"
                     /locus_tag="WM481_002112"
                     /gene_synonym="lytB"
     CDS             complement(126..1094)
                     /gene="ispH"
                     /locus_tag="WM481_002112"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFA9081330.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPVYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQNCEIIMIGHKGHAEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNASYLQRTWFEGKSKVGVTAGASAPEVLV
                     REVLAAIRGWGHETVREGGGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1124..1618)
                     /locus_tag="WM481_002113"
     CDS             complement(1124..1618)
                     /locus_tag="WM481_002113"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HFA9081331.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKIGAAM
                     IIGGASGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     SKKT"
BASE COUNT          371 a          502 c          433 g          392 t
ORIGIN      
        1 ggaagaaggt gctcatggga aatcccctaa atgccttggt gggaatttag gggattttgg
       61 ggatttttgc aaaggtctcg gcatttgtat tgcccaaaca tccgatgcct gcgttaacgc
      121 ctgcgtcaac gtttgcacaa atcgggtttg gtttcgccct cgcggcgcag ctccttgggc
      181 aggacgaaca cgatgctttc ttccgcgccc cccccttcgc gcacggtttc atgcccccat
      241 ccgcgtatgg ctgccaatac ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt
      301 acgccgactt tgcttttgcc ttcaaaccac gtgcgttgca ggtaggacgc gttgtccacc
      361 atatacgcat cgattccgcg cgatgccgcc acttcgcgca ggcggttgct gttggacgaa
      421 ttgggcgaac cgaccacaat cacgatgtcg cactgttccg ccagctcttt gacggcggtt
      481 tgccggttgg tcgtcgcata gcagatgtct tccttgtgcg gattgcggat attggggaaa
      541 cgcgcgttca gcgcggcgat gatgtctttg gtttcatcga ccgagagcgt ggtttggctg
      601 acataggcga gtttgtcggg gtttctgact tcgagttttg ccacatctcc gaccgtttcg
      661 accaaaagca ttttgcccgg tgcaagctgc cccatcgtgc cttcgacctc ggcgtgcccc
      721 ttatgcccga tcatgatgat ttcacagttt tgggcatcca gtcgggcgac ttccttatgc
      781 actttcgtta ccagcgggca agtcgcatca aatacccgga aaccgcgctc cgccgcttcc
      841 tgctgcaccg ccttcgatac gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact
      901 tccgccaagt cttcgataaa caccgcgcct ttttcgcgca ggttgtccac gacgaatttg
      961 ttgtggacga cttcgtggcg cacataaacc ggcgcgccga attcttccaa agcacgttcg
     1021 acaatactga ttgcccgatc cacaccggcg cagaagccgc gcggattggc aaggatgatg
     1081 gtttttccgt tcataagttt tgcattccgt gttcagacgg cattcacgtt tttttgctat
     1141 ctttgcgatg gacgatattg tcaagcaccg ccaacaccgc accgacgcag ataaagctgt
     1201 cggcaatatt aaaggcggga taaaaccaat tttgccaata aaacaataag aaatcgacga
     1261 catgaccgtg tatcaggcga tcgatgacat tgcccgacgc accgccgata atcattgccg
     1321 ccccgatttt gccgagggct gcaaactcgt cgcgcaagat ggcgcgtacc aaatacgcgc
     1381 tcaccgccac cgccagcacc aaaaaaaagt atttctgcca gccgccctga tcggcaagga
     1441 agctgaatgc cgcgcccggg ttgtacacca gcgtcagatc gaaaaacgaa gggatgacgt
     1501 tgacgcgttc ccgatactga aacgacgaca gcaccgccca cttcgaccac tggtccagca
     1561 cgatggcggc aagtgccaat acccaatagc gcgttttact tgaaacagat gaagacatat
     1621 ttttcaacag ccggataaaa gagtgccatt ctacccgaaa accccctttc ctgtacccga
     1681 aacggcaaat gccgtaat
//
LOCUS       DAWTCI010000140         1658 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWTCI010000140 DAWTCI010000000
VERSION     DAWTCI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.140"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..668
                     /locus_tag="WM481_002114"
     CDS             <1..668
                     /locus_tag="WM481_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFA9081332.1"
                     /translation="EVLSEEKDGNRLARHHKHGEIWKKPTRHEDIAAVFPFGNPIHNN
                     TMIMRRSVIDGGLRYDTGRDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVSSKH
                     SVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQTKAAAYELPEKDLPEEDFERAR
                     RFLYRCFKRTDTPPSGAWLDFAADGRMRRLFTLRQYFGILYRLIKNRRQARSDSAGKE
                     QEI"
     gene            668..1507
                     /locus_tag="WM481_002115"
     CDS             668..1507
                     /locus_tag="WM481_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFA9081333.1"
                     /translation="MQNHVISLASAAERRAHIADTFGSRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWEQALDEGLPYIAVFEDDVLLGEGAEQFLA
                     EDTWLQERFDPDSAFVVRLETMFMHVLTSPSGVADYGGRAFPLLESEHCGTAGYIISR
                     KAMRFFLDRFAVLPPERLHPVDLMMFGNPDDREGMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRRLNRKQQWRDSPANTFKHRLIRALTKIGREREKRRKRREQTIGKIIV
                     PFQ"
     gene            1519..>1658
                     /locus_tag="WM481_002116"
     CDS             1519..>1658
                     /locus_tag="WM481_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081334.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEEN
                     RAA"
BASE COUNT          403 a          458 c          461 g          336 t
ORIGIN      
        1 tggaagtttt gtcggaagaa aaggacggca accggctggc gcggcaccac aaacacggcg
       61 aaatttggaa aaagccgacc cggcacgaag acatcgccgc cgttttccct ttcggcaacc
      121 ccatacacaa caacacgatg attatgcggc gcagcgtcat tgacggcggt ttgcgttacg
      181 acaccgggcg ggattgggcg gaagattacc aattttggta cgatgtcagc aaattgggca
      241 ggctggctta ttatcccgaa gccttggtca aataccgcct tcacgccaat caggtttcat
      301 ccaaacacag cgtccgccaa cacgaaatcg cgcaaggcat ccaaaaaacc gccagaaacg
      361 attttttgca gtctatgggt tttaaaaccc ggttcgacag cctagaatac cgccaaacaa
      421 aagcagcggc gtatgaactg ccggagaagg atttgccgga agaagatttt gaacgcgccc
      481 gccggttttt gtaccgatgc ttcaaacgga cggacacgcc gccctccggc gcgtggctgg
      541 atttcgcggc agacggcagg atgaggcggc tgtttacctt gaggcaatac ttcggcattt
      601 tgtaccggct gattaaaaac cgccggcagg cgcggtcgga ttcggcaggg aaagaacagg
      661 agatttaatg caaaaccacg ttatcagctt agcttccgcc gcagagcgca gggcgcacat
      721 tgccgatacc ttcggcagtc gcggcatccc gttccagttt ttcgacgcac tgatgccgtc
      781 tgaaaggctg gaacaggcga tggcggaact cgtccccggc ttgtcggcgc acccttattt
      841 gagcggagtg gaaaaagcct gctttatgag ccacgccgta ttgtgggaac aggcgttgga
      901 cgaaggctta ccgtatatcg ccgtatttga agatgatgtc ttactcggcg aaggcgcgga
      961 gcagttcctt gccgaagata cttggctgca agaacgcttt gaccccgatt ccgcctttgt
     1021 cgtccgcttg gaaacgatgt ttatgcacgt cctgacctcg ccctccggcg tggcggacta
     1081 cggcgggcgc gcctttccgc ttttggaaag cgaacactgc gggacggcgg gctatattat
     1141 ttcccgaaag gcgatgcgtt ttttcttgga caggtttgcc gttttgccgc ccgaacgcct
     1201 gcaccctgtc gatttgatga tgttcggcaa ccctgacgac agggaaggaa tgccggtttg
     1261 ccagctcaat cccgccttgt gcgcccaaga gctgcattat gccaagtttc acgaccaaaa
     1321 cagcgcattg ggcagcctga tcgaacatga ccgccgcctg aaccgcaaac agcaatggcg
     1381 cgattccccc gccaacacat tcaaacaccg cctgatccgc gccttgacca aaatcggcag
     1441 ggaaagggaa aaacgccgga aaaggcgcga acagacaatc ggcaaaatta ttgtgccttt
     1501 ccaataaaag gagaagagat ggacatcgta tttgcggcag acgacaacta tgccgcctat
     1561 ctttgcgttg cggcaaaaag cgtggaagcg gcccatcccg atacggaaat caggttccac
     1621 gtcctcgatg ccggcatcag tgaggaaaac cgggcggc
//
LOCUS       DAWTCI010000141         1637 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWTCI010000141 DAWTCI010000000
VERSION     DAWTCI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.141"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(57..>1637)
                     /locus_tag="WM481_002117"
     CDS             complement(57..>1637)
                     /locus_tag="WM481_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HFA9081335.1"
                     /translation="GVFSHDLWNPAEPAVYTAVEALAGSPTPLVAHLFGQTDFGIPPV
                     YLWVAAAFKHLLSPWAADPYDAARFAGVFFAVIGLTSCGFAGFNFLGRHHGRSVVLIH
                     IGCIGLIPVAHFLNPAAAAFAAAGLVLHGYSLARRRVIAASFLLGTGWTLMSLAAAYP
                     AAFALMLPLPVLMFFRPWQSRRLMLTAVASLAFALPLMTVYPLLLAKTQPALFAQWLN
                     YHVFGTFGGVRHIQTAFSLFYYLKNLLWFALPALPLAVWTVCRTRLFSTDWGILGIVW
                     MLAVLVLLAFNPQRFQDNLVWLLPPLALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAV
                     FLWTGFFAMNYGWPAKLAERAAYFSPYYVPDIDPIPMAVAVLFTPLWLWAITRKNIRG
                     RQAVTNWAAGVTLTWALLMTLFLPWLDAAKSHAPVVRSMEASFSPELKRELSDGIECI
                     GIGGGDLHTRIVWTQYGTLPHRVGDVRCRYRIVRLPQNADAPQGWQTVWQGARPRNKD
                     SKFALIRETGENILKTTD"
BASE COUNT          410 a          478 c          489 g          260 t
ORIGIN      
        1 aaaggtttaa tcgcgcaatt atacgcgatt tccggcactt aatccagaaa ttcggctcaa
       61 tctgttgttt ttaatatatt ttccccggtt tcccgtatca gtgcaaactt actgtctttg
      121 ttgcgcgggc gcgcaccctg ccagaccgtc tgccagcctt gcggcgcatc cgcgttttgg
      181 ggcaggcgga cgatacggta gcggcaacgg acatcgccga cgcggtgcgg caatgtgccg
      241 tactgcgtcc aaacaatccg cgtgtgcagg tcgccgccgc ctatgccgat acactcgatg
      301 ccgtctgaaa gctcccgttt taattccggg gaaaacgatg cctccatact ccggacgacg
      361 ggcgcgtggc ttttcgccgc gtccagccac ggcaggaaca gcgtcatcag caaagcccag
      421 gtcagggtaa cgcctgccgc ccagttggta accgcctgcc tgccgcgtat gtttttccgg
      481 gtaatcgccc acagccacaa gggtgtgaac agtacggcaa ccgccatcgg aatgggatcg
      541 atgtcgggaa cgtaatacgg gctgaagtag gcggcgcgtt cggcaagctt ggcgggccag
      601 ccgtaattca tggcgaaaaa gcccgtccac aggaacacgg caaacagccc gaacgccata
      661 atgccgaacc agttgacaaa agccgccgcg ccgcgcctca ggctgtccag ttgcgccgcg
      721 ccgaacaggg caagcggcgg cagcagccag acgaggttgt cttgaaaacg ctgcggatta
      781 aaggcgagca gcaccaaaac ggcaagcatc cagacaatgc ccaaaatccc ccagtcggtc
      841 gaaaacaggc gtgtgcggca aaccgtccaa accgccagcg gcagcgcggg cagtgcgaac
      901 caaagcagat ttttcagata gtaaaacaaa ctgaatgccg tctgaatgtg ccgcacgccg
      961 ccgaacgtac cgaaaacgtg atagttgagc cattgcgcaa acagcgcggg ctgcgttttt
     1021 gccaagagca gcgggtaaac ggtcataagc ggcagggcaa aggcaagcga ggcgactgcc
     1081 gtcaacatca aacgcctgct ttgccacgga cggaaaaaca tcagcacggg caggggcagc
     1141 atcagcgcaa acgccgccgg ataagctgcc gccagcgaca tcaacgtcca acccgtaccg
     1201 agcaggaaag aggcggcaat cacgcgccgg cgtgccagcg agtagccgtg cagcaccagt
     1261 ccggcggcgg caaaggcggc ggcggcggga ttgaggaaat gggcaaccgg aatcagcccg
     1321 atacagccga tatggattaa aacaacgctg cgcccgtggt gtctgcccaa aaagttgaaa
     1381 ccggcaaagc cgcaagaagt cagtccgata acggcaaaaa atacgcctgc aaagcgtgcg
     1441 gcatcatacg ggtcggctgc ccacggcgac agcaaatgtt tgaatgcggc ggcaacccaa
     1501 agatacacgg gcggtatgcc gaaatcggtt tgaccgaaca gatgggcaac caagggggtg
     1561 gggctgcctg ccagtgcttc gacggcggta tagacggcag gttcggcagg attccacaaa
     1621 tcgtgggaaa acacgcc
//
LOCUS       DAWTCI010000142         1630 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWTCI010000142 DAWTCI010000000
VERSION     DAWTCI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.142"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(<1..749)
                     /locus_tag="WM481_002118"
     CDS             complement(<1..749)
                     /locus_tag="WM481_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HFA9081336.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLEKSQIKFQSSNNLKSI
                     GFKLDSSSNTFSKFTEAANFEHIPTGAKARINAKITASVSRAGVLSGVGKLVRQGAKF
                     GTRAVPYVGTALLAHDVYETFKEDIKARGCRYDPETDKFVKGYEYANCLWYEDERRIN
                     KTYGCYGVDSSIMRLMPDRSRFPEVKQLMESQMERIARPFWDKRKEEIPKDTALKWRL
                     FPFSHCEFFWNGGGCFVRNGDNLIHRVYFKL"
     gene            complement(691..1008)
                     /locus_tag="WM481_002119"
     CDS             complement(691..1008)
                     /locus_tag="WM481_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFA9081337.1"
                     /translation="MNKPFITQAQLALYKYQPSSEYFGQSMAFIAQKEFEEFVNNVKE
                     YDILESFSYFLNKRVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNS
                     MFE"
     gene            complement(1136..1414)
                     /locus_tag="WM481_002120"
     CDS             complement(1136..1414)
                     /locus_tag="WM481_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081338.1"
                     /translation="MYCQVGNKCLEKHRAENLYFSLVVPRIQENGQIIRPEYNGSMWK
                     MSDGQPLRLSLAECSPKDNLQSGLETGRIVFGVLASVYFVSLLKKVLK"
     gene            complement(1421..>1630)
                     /locus_tag="WM481_002121"
     CDS             complement(1421..>1630)
                     /locus_tag="WM481_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081339.1"
                     /translation="INTCRKYGAKLAVVTAAPLALAAQANAALPETAKNALEAAKADG
                     MEAGWIVVGVFAALFVFSIVKRVMK"
BASE COUNT          444 a          378 c          325 g          483 t
ORIGIN      
        1 tcaagtttaa aataaactcg atgaattaaa ttatcaccgt tacgtacaaa acaaccgccg
       61 ccgttccaaa aaaattcaca atgactaaaa ggaaaaagac gccactttaa agcagtatct
      121 ttaggaattt cttctttccg tttatcccaa aaaggacgag caatcctttc catttgactt
      181 tccatcagtt gtttgacttc ggggaatctg ctgcgatcgg gcataaggcg cataatcgaa
      241 ctgtcaacgc cgtagcagcc ataggtttta ttaatacgtc tttcgtcttc gtaccaaagg
      301 caattagcat attcgtagcc ttttacaaat ttgtcggttt cgggatcgta tcggcagcct
      361 cgtgccttta tgtcttcttt gaaagtttcg tatacgtcgt gggctaaaag ggctgttccg
      421 acatagggaa ccgcccttgt gccgaatttc gcgccttggc ggacaagttt gccgaccccc
      481 gacaatacgc cggcgcggga tacgctggcg gttattttgg cgttgattcg ggcttttgcg
      541 cccgtgggga tgtgctcgaa atttgccgct tctgtgaatt tggaaaaagt gttagaactt
      601 gaatctaatt taaaaccaat tgattttaaa ttattacttg attgaaattt aatttgtgat
      661 ttttctaatc ttacaggttc agaataagaa ttactcgaac atagaattaa aatcagaact
      721 atcggtgtac tcttgataaa cgaattcatg aacagttttt ccgttctctt ctgattttct
      781 tataaaaata acagactcat cagaaaaata aatcttccaa atattatgtg cgaccctctt
      841 atttaagaaa taagagaagc tctctaaaat atcgtactct tttacgttat taacaaattc
      901 ttcaaattct ttctgagcaa taaaagccat cgattgccca aaatactcgc ttgacggctg
      961 atatttataa agtgccaact gcgcctgcgt gataaacggc ttgttcatgg ttctgccttt
     1021 caaaggttgt tttgaaagcc tgattttgac accataactt catgcgctca atccttaaac
     1081 agaaccgccc cgattaatac ggggacggca acgccgagat agaaataaaa atccatcatt
     1141 tcaaaacctt tttcagcagg gaaacaaagt aaacggacgc gaggacgccg aatactatcc
     1201 ggcctgtttc aagaccgctt tgcaggttgt ctttcggact gcattccgcc aatgaaagcc
     1261 ttagcggctg accgtccgac atcttccaca tgctgccgtt atattccggc ctgattatct
     1321 gtccgttttc ttggattctt ggtactacca agctgaaata aaggttttcg gcccggtgct
     1381 tctcaagaca tttatttccg acttggcagt acatgccgcc ttacttcatc accctcttaa
     1441 cgatggaaaa tacaaaaagc gcggcgaaaa cgcccactac aatccaaccg gcttccatac
     1501 cgtccgcttt tgcggcttcc aaagcgtttt ttgccgtttc gggcaacgct gcgtttgcct
     1561 gtgccgccaa agccagcggg gcggctgtta caacagccag ttttgcgccg tatttacggc
     1621 aggtgttaat
//
LOCUS       DAWTCI010000143         1594 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWTCI010000143 DAWTCI010000000
VERSION     DAWTCI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.143"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            165..655
                     /locus_tag="WM481_002122"
                     /pseudo
     CDS             165..655
                     /locus_tag="WM481_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            692..958
                     /locus_tag="WM481_002123"
                     /pseudo
     CDS             692..958
                     /locus_tag="WM481_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1460..>1594
                     /locus_tag="WM481_002124"
     CDS             1460..>1594
                     /locus_tag="WM481_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFA9081340.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLISPLAHAGAQREETLADDV
                     A"
BASE COUNT          389 a          382 c          437 g          386 t
ORIGIN      
        1 gtcctagatt cccacttccg tgggaatgac ggttcagcaa gcgtaggtcg gatacttgta
       61 tccgacaaaa cctttaacat tcccatcatt gcaatccatt gcagcaatgt ccaaaatgtc
      121 gaattcaaga atccggccta caaaatcatt ccgagcataa tactatgaaa taccgtcgtt
      181 tttaccgcaa tggcggcact tactttttta cggttgtaac caataaacgg cagaagattt
      241 tgaccgatga tgcggtgcgt ttggctttac ggcaggcggt aatggcggtg cgcgaacgga
      301 atccgtttga aattttggca tgggtgttga tgcccgatca tctgcatacc atatggcggc
      361 tgccggacaa tgattctgct tattcggaac gctggcggca aatcaagcgg cacagccaat
      421 atttaatcgg cggcaatctc aggctttgga aaaacgcttt tgggaacata ctatccgcgg
      481 tgaggccgat tttgcctgcc attttgatta tctgcatttc aatccggtca aacatggcta
      541 tgtaggacaa atttccgatt ggcggttttc tacgtttcac cgttatgtca aacagggtat
      601 ttatccgcat aattggggtg gcggcaatgc ggacttttct attgaatacg attgaagtaa
      661 tgtcggattc gagaatccga cctacggaaa actgaaagag catcggctgc tggacggcat
      721 tatgcgcaaa gcccgccgca accgcccgct gccggaggcg caaaccaaac gcaaccgata
      781 tttgtcgaag acccgttatg tggtcgaaca aagcttcggt acgctgcacc gtaaattccg
      841 ctatgcccgg gcggcctatt tcggactgat taaagtgagt gcgcaaagcc acctgaaggc
      901 gatgtgtttg aacctgttga aagcggccaa caggctaagt gcgcccgctg ccgcctaaaa
      961 ggcggcccgg atgcctgatt atcgggtatt caagggggat taagggggta tttgggtaga
     1021 gttaggagtg attggtggcg gaaacagccg aaaatctgtg tcagggtttc agctgtcggg
     1081 agggaaggga attttgcaaa ggtctcaaga tatagtggat taaatttaaa ccggtacggc
     1141 aaggcgaggc aacgccgtac cggtttaaat ttaatccact atatttccgc ctgctgccgc
     1201 ccgaaaagcg taatgcgctg atggtgctgc tgcacaatcc ggacgacgga taaccggtga
     1261 aaacgggaaa tgcccgggcg gtgtcgtctg tgcctgtttg gacaagcatt cttgagggcg
     1321 gtaaaatcaa ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga ttttgcgcgc
     1381 ggtttttcgc gttggaagca atcatgtctg tttgaatatt taatgccgtc tgaaacgtcc
     1441 ggacgcaccc ccgttcctta tgaaaaaacc gaccgatacc ctacccgtca atctgcaacg
     1501 ccgccgcctg ctgtgtgccg ccggcgcgct gttgatcagc ccgctggcgc acgccggcgc
     1561 gcaacgtgaa gaaacgcttg ccgacgatgt ggct
//
LOCUS       DAWTCI010000144         1488 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWTCI010000144 DAWTCI010000000
VERSION     DAWTCI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.144"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(10..269)
                     /locus_tag="WM481_002125"
                     /pseudo
     CDS             complement(10..269)
                     /locus_tag="WM481_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            457..801
                     /locus_tag="WM481_002126"
     CDS             457..801
                     /locus_tag="WM481_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFA9081341.1"
                     /translation="MNLPFRAMVSDLGGTLLTPEHLVGDLTIDTLRVLEQKGVDIILA
                     TGRNHTDMSSILGKIGAERAVMITSNGARVRDLQGNLLYSNSLPEELVLELYKTSYVG
                     NLIQIPRFCRHQ"
     gene            755..1288
                     /locus_tag="WM481_002127"
     CDS             755..1288
                     /locus_tag="WM481_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFA9081342.1"
                     /translation="MWEILFRYQDFVAINKPQGISVHRSGGEVSLTATLAAQLGVEKV
                     WLLHRLDKQAGGILLFALNPQSAAVLAAQFAERKMKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNMENIAVTRFFSIRISEKMRLFILEPHTGKTHQLRVVMKSLGSSIF
                     GDSLYGGTESETMFLYA"
BASE COUNT          453 a          309 c          305 g          421 t
ORIGIN      
        1 gtacagcccc tatttatttt gtgccttact ttcagtacca attgccaccg cacttaaatt
       61 tgttgcctga gaagatgtcc ccactgcaat tcctttcgta ccactggcag cactgctttt
      121 ccctaaggaa ataccaaaat tcgatgcatt agcagaagta cccaatgcca ctcaatttta
      181 gatgccgatg ctttatcgtt ccatgataag gagccattat taccttcttg caaagtagaa
      241 tccgccccat aagaagacaa agcactcatt aacaaaatac cagccgctat cgcggacgac
      301 ctatatacat aacattactt catatcattt cattcagtat caatagtata ggataactcc
      361 cccccccgtc aatcgaaata attcccgtat tttaatgtcg tattgttcct gtatatttag
      421 tgatgtgata agtatagttt attttttgag gtttttatga atttaccttt tcgggcaatg
      481 gtgtctgatt taggtggcac acttttaaca cctgaacatt tagtgggtga tcttaccatt
      541 gatactctga gagtattaga gcaaaaaggc gtagatatta ttttggcaac aggacgcaat
      601 cataccgata tgtcatccat tcttggaaaa atcggtgcgg aacgtgcggt tatgattaca
      661 tcaaacggtg ctcgtgtacg ggatttacag ggtaatttgc tttatagcaa tagtttgcct
      721 gaagaattag ttctcgagct ttacaaaaca tcatatgtgg gaaatcttat tcagatacca
      781 agattttgtc gccatcaata aacctcaagg catatccgtc caccgttccg gcggcgaagt
      841 cagtctgacg gcaacacttg cggcacagtt gggcgtagaa aaggtatggt tgctgcacag
      901 gttggacaag caggccggag gcatattgct gtttgccctt aatccccaaa gtgccgctgt
      961 tcttgccgca cagtttgccg aaaggaaaat gaagaaaacc tatttggctt tgtccgaccg
     1021 caaaccgtcc aaaaaacaag gatggataaa aggcggcatg gaaaaatcca ggcgcgggat
     1081 gtggaagctg acgcgcaata tggaaaatat tgccgttacc cggtttttca gtatccgcat
     1141 cagtgaaaaa atgcggctgt tcatccttga gccacatacg ggtaagacac atcagttgag
     1201 ggtggtaatg aagagtctgg gcagctctat atttggggac agcctgtatg gaggaacaga
     1261 atccgaaact atgttcctgt atgcgtgaaa aatacaattt acctatcaac aacaagaaat
     1321 cgaaattacc gcacctttga aaaatgcatg gcaggccgaa aatatctctc atgcactgga
     1381 agaattttat atggcaaaag caggttaata gatataaatc aattaaaaca atatgataaa
     1441 tatttttata aaattttttc aaaaaacggt tgaccgcaaa tctcttta
//
LOCUS       DAWTCI010000145         1347 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWTCI010000145 DAWTCI010000000
VERSION     DAWTCI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.145"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(12..209)
                     /locus_tag="WM481_002128"
     CDS             complement(12..209)
                     /locus_tag="WM481_002128"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFA9081343.1"
                     /translation="MDNAAMESFFGTLKSECFHTCKYDSVTESEAALHEYIRYYNNDR
                     IKLKLKGLSPVQYRIQSLKAA"
     gene            complement(254..832)
                     /locus_tag="WM481_002129"
     CDS             complement(254..832)
                     /locus_tag="WM481_002129"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFA9081344.1"
                     /translation="MPSVELLLGIVGLPRSTFYYQLAVQSAEGKYADLKRHIHDIYQR
                     YKGRYGYRRIAAAIRHAGIPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKVAPNI
                     LQRCFHSEKPNEKWVTDVTEFDVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPGPSEKPMLHSDQGWQYQMFFIKSS"
     gene            complement(837..1265)
                     /locus_tag="WM481_002130"
     CDS             complement(837..1265)
                     /locus_tag="WM481_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9081345.1"
                     /translation="MSDSLVRKWVARYRLHGESGIKRRKHTTKYSVEYKLEAIRPVTG
                     QGMSQKAAADQLNLPDCSVLPQWLRLYRLNGINGLKPKPKGRKPVKKQYPPQTKKADY
                     LKTKEELFAELAYLKAEAAVLKKLDALKEVRQKERNSSQG"
BASE COUNT          299 a          343 c          311 g          394 t
ORIGIN      
        1 ggacagttta atcaagcggc tttcagggac tgaattctgt actgaacagg gctcagtcct
       61 tttaatttca acttgattct atcgttgttg tagtaacgga tatattcgtg cagtgccgct
      121 tccgattcgg taacggaatc atatttgcac gtatggaaac attccgattt caacgttccg
      181 aagaaacttt ccattgccgc attgtccaag cagtttccct tgcgggacag actctgaacc
      241 agaccgttgc ctttcaactg cttttgataa aaaacatctg atattgccaa ccttgatccg
      301 aatgcagcat cggcttttca gacggccccg gtttctccgg cgcacctttc agtatctcgc
      361 cggccaaatc gaaagtcggg cgggtttgaa tacggtaact gacgatttcc ccgttaaaca
      421 aatccataat cggagaaagg tatatctttt ctccgcctac atcgaactcg gtaacgtccg
      481 ttacccattt ctcattcggc ttttctgaat ggaaacagcg ttgcaggatg ttcggcgcaa
      541 ctttgccgac ttctcctttg aacgagcggt atttgcgccg ccgtatcact gccttcagcc
      601 ccgtcttcgc catcagacgg ctgactttct tgtgattgac cggtattcct gcgtgacgga
      661 tggctgccgc aatcctccgg tagccgtatc ttcccttata tcgttgataa atatcatgga
      721 tatgtcgttt caaatcggca tatttgcctt ctgccgattg gacggccaat tggtaataga
      781 aggtgctgcg tggcagtccg acaatcccca acagcagttc aacggatggc atttccttaa
      841 ccctgcgacg agttgcgttc tttctgccgc acttctttca aggcatcgag cttttttagg
      901 acagccgctt ccgctttaag gtaagccaat tccgcaaaca gttcttcctt ggttttcaga
      961 tagtcggctt ttttcgtttg cggcggatac tgttttttca cgggctttct tcctttgggt
     1021 ttgggcttta aaccgttaat accattcaaa cggtagaggc gcaaccattg cggcaaaacg
     1081 gagcagtcgg gcagattcag ttggtctgcg gcagcttttt gggacattcc ctgccccgtc
     1141 accgggcgga ttgcctcaag tttgtattcg accgaatatt ttgtcgtatg ctttctgcgt
     1201 ttgatgccgc tttcaccgtg taatctgtat cttgccaccc atttgcgtac caatgaatcg
     1261 gaaatggaaa aatatagtga attaacaaaa atcaggacaa ggcggcgggc cgcaggcagt
     1321 acaaatggta cggaaccgat ccgcccg
//
LOCUS       DAWTCI010000146         1191 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWTCI010000146 DAWTCI010000000
VERSION     DAWTCI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.146"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(36..959)
                     /locus_tag="WM481_002131"
     CDS             complement(36..959)
                     /locus_tag="WM481_002131"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFA9081346.1"
                     /translation="MLQRTLAKSIGVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLNPYLINDTRLSSTIVTDKGLRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSPPTFEIDFAGKSYIGEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IGDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAVNNALLRAV
                     LADETAYEWVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          290 a          284 c          328 g          289 t
ORIGIN      
        1 atctgtttga cggcatttgt ttggaaaagg gggcgttatc cgcaatttct gatgttcagc
       61 tcgtgaaatg cgtcgggcaa atcatcgctg tcggcaaatt ccacccattc gtaagccgtt
      121 tcgtctgcca aaactgcgcg caaaagggcg ttgttgacgg cgtgtcccga tttgtagcct
      181 tcaaacgcgc cgacaatcgg atgtccgacg atatacaaat cgccgatggc gtcgaggatt
      241 ttgtggcgca caaactcatc gggatagcgc aagccttcag gattcaggac atccgtgtcg
      301 ccaatcacga tggcgttgtt caaattgccg cctaagccga gattgtgggc gcgcatcatt
      361 tccacttcgt gcataaagcc gaaagtgcgc gcgcgcgcga tttcgccgat gtaggatttg
      421 ccggcgaaat caatttcaaa cgtaggcggg ctgcggttga aaacaggatg gtcgaattcg
      481 atggtcagcg ttaccttaaa accgtcatac ggcgtaaatc ggacccattt gcccgcttct
      541 ttgatttcga cgggtttgag gattttcaaa aaacgctttt gcgccttttg gtctacaacg
      601 cccgcatctt gcaggaggta aataaacggc aggctggagc cgtccataat cgggatttcg
      661 ggcgcgttca actcaatcag cgcattgtcg atgccgtagg cggacagtgc ggacatgatg
      721 tgttcgatgg tgccgacgcg caggcctttg tcggtaacga tggtggatga aaggcgggta
      781 tcgttgatca aataagggtt cagcttgatt tgttcgccca tctcgccgtc caaatcggtg
      841 cggcggaagg aaatcccgct gttttcaggc gcggggtgca gggtcagcgc gacgcgttcg
      901 cccgaatgca gcccgactcc ggtaacgccg atggatttcg ccaaagttct ttgcagcata
      961 aaccgcttcc ttatcaatgg ggtaagtttc ggaataatac gataaaaccg gaaaaacagg
     1021 ctatgttttt caatagtatt tgccaatgta tccgtttcca acacgtaagc cgcatgaaaa
     1081 tgtatggcgg attaacgaaa atcaggacaa ggcggcgaag ccgcagacag tacggatagt
     1141 acgaaaaggc gaggcaacgc cgtactggtt taaatttaat tcactataca t
//
LOCUS       DAWTCI010000147         1157 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWTCI010000147 DAWTCI010000000
VERSION     DAWTCI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.147"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(51..335)
                     /locus_tag="WM481_002132"
     CDS             complement(51..335)
                     /locus_tag="WM481_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081347.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFGGKPADEARFAAVLTETCRRAVKKIGSVPD"
     gene            complement(372..635)
                     /locus_tag="WM481_002133"
     CDS             complement(372..635)
                     /locus_tag="WM481_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081348.1"
                     /translation="MADILPSDKRRQPPKTKGNPINIIEQETKRTELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(655..903)
                     /locus_tag="WM481_002134"
     CDS             complement(655..903)
                     /locus_tag="WM481_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081349.1"
                     /translation="MHGFRDLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHH
                     YLTADSYRLQERRELAEWYGRRHRQAYEAAAQNTCNKT"
     gene            complement(864..>1157)
                     /locus_tag="WM481_002135"
     CDS             complement(864..>1157)
                     /locus_tag="WM481_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFA9081350.1"
                     /translation="TARLWHIPAADMKTKHPFTVPLSDWAVEILRELHTQTGDNIHLF
                     PGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTPANPNTPCTDSATSSPTSA"
BASE COUNT          181 a          239 c          401 g          336 t
ORIGIN      
        1 ttctttaatt tgcagtgtgt tatcttcata tttcgagggt aacatatctg ctaatctggt
       61 acagacccta tttttttgac tgcccggcgg caggtttcgg taaggacggc ggcaaatcgg
      121 gcttcgtctg ccggtttgcc gccgaaaaaa ataaaatcgt aaagggtgat gccgttttcg
      181 gaatggcggt aatgcccgcc cgggcattcg ccgtctgtct gcctgtggat tttggcaatc
      241 aggcggggat agcggcaatg ggtaatgaaa cggcttgcgc cgtcttgccc gataatccat
      301 tcggggtagc ggttgaatag tgcggctctg ttcattttgt tgagggataa agcccctcgc
      361 ggggcttgtg gtcaggcaaa tttgaatatc agtgccaaca cggcggcgat ggcggtaacc
      421 agaccggtgg ccgccatcat gggataccag cgggactctt gggctatttt tacggattcg
      481 gcgttgattt tgtgcgcgtc ggcaatgatt ttggcgattt cggcatctat ttttcttaac
      541 tcggtgcgtt ttgtttcttg ttcgattatg tttattggat ttcctttcgt ttttggcggt
      601 tgccgccgct tgtcggatgg caggatgtct gccatgtatg tatgttaata ccttttaggt
      661 tttattgcaa gtgttttggg cggcggcttc gtatgcttgg cggtggcggc ggccgtacca
      721 ttcggcgagt tcgcgccgct cttggaggcg gtagctgtcg gcggtcaggt agtggtgcag
      781 gcttgagaag ccggcgcgtt ggagggcggc gcggctgatg tggccaaggg cgaggtctgt
      841 tgtgagggtg tcttttccgg cggtcaggct gatgttggtg aagaggtcgc ggaatccgtg
      901 catggtgtgt ttggatttgc cggggtgctg ccgtcatagc ccagtcgtcg gatggcgttg
      961 tgggcgaatt tgatgctgat gtggtcggga tggggggcgg gtttgcgccg tgggcggatg
     1021 cctgggaaaa ggtggatgtt gtcgccggtc tgtgtgtgca gctctcggag tatttctact
     1081 gcccagtccg acagtgggac ggtaaagggg tgtttggtct tcatgtcggc ggcggggatg
     1141 tgccataacc gggcggt
//
LOCUS       DAWTCI010000148         1144 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWTCI010000148 DAWTCI010000000
VERSION     DAWTCI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.148"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WM481_002136"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WM481_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFA9081351.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELATALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEKGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          300 a          322 c          293 g          229 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgct gctttgacta ctattttagc taaaaaattc ggcggcgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtagaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg gtcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 tgctgccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaactggcta ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacca ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gcggtaccgt
      661 agtcaccggc cgtgtagagc gaggtatcat ccacgttggt gacgagattg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca agttcaaagc
      901 agaagtgtac gtattgagca aagaagaggg cggccgccat accccgtttt tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccactga cgtaaccggc gcggttactt tggaaaaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattact gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggtctgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWTCI010000149         1142 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWTCI010000149 DAWTCI010000000
VERSION     DAWTCI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.149"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(99..422)
                     /locus_tag="WM481_002137"
     CDS             complement(99..422)
                     /locus_tag="WM481_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081352.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEHDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(425..613)
                     /locus_tag="WM481_002138"
     CDS             complement(425..613)
                     /locus_tag="WM481_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HFA9081353.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNINKVEI
                     HYIENTRTGEKTDFKFKD"
     gene            complement(681..1019)
                     /locus_tag="WM481_002139"
                     /pseudo
     CDS             complement(681..1019)
                     /locus_tag="WM481_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT          286 a          216 c          189 g          451 t
ORIGIN      
        1 tatttttatt tccagtcatt ctggctattt tatgtatatt tccattatgt ttgattatca
       61 ttttattttt tccaaggatt ttcttctata ttgatagttt atctaaagat ttcatttaaa
      121 atatcctgac taatttcttg tttatataat tctacaattt tctgtttctg tgtatttgta
      181 taaaattttt cgttggtatc tgcttgatat tttaattgtc tcaatatttc catgatttca
      241 tattctgatt tttcatcatg ttcttcaatc atgtaatcaa caaattgttt tagaatatca
      301 aaggcttcgt aactttccaa aattttaata tgtttatcgg caaaattgga catataccat
      361 tcgtcagtga ttggattggt atttaaatca tctaaaaaaa catttacatc atctaaaaac
      421 atggctaatc cttaaacttg aaatctgttt tctcaccggt tcttgtattt tcaatgtaat
      481 gtatctcaac tttgtttata ttttgaacgc gcttaaccca gccgtctttt gccaaccaac
      541 catttttagt atcggacata ggaggtattc tcgcaggagt tttgccctgt gggtttgcct
      601 taacttcttc cattgctaat tgttccttta aattattagg tattgtacga ccggttgatt
      661 tttcaacaga ttgtgtccct atttttaatg atttgtttcc ccccagccat ttccctgtct
      721 acagtctgca tatggcgggc agtaggtttt tcatagccgg tatctttaac tcaatcccat
      781 ttagtcgggt taagttctac ttttttacct ctccaaaccg tacctgcggc ctctgctacg
      841 gcaagggcag tttgggcagt agctgtaatg ttcggatggg catcagccca ttgtctggca
      901 gagttgatac cgtcttttac cgcaaaagca ctgtcctgta ataggctcac ggcggcaagt
      961 tgtgcttccg gacttaattt tcctgaatta ttaatacctt gtagagtctg ctgcgcagag
     1021 atgtagcaac tgtattttta ccccgtcggg taaaaataca gttgctacgg ctgctgggaa
     1081 agcagttact attttcccag tggctcaaaa ttaattgttt cagcagatat ttttataata
     1141 at
//
LOCUS       DAWTCI010000150         1097 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWTCI010000150 DAWTCI010000000
VERSION     DAWTCI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.150"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(246..656)
                     /locus_tag="WM481_002140"
     CDS             complement(246..656)
                     /locus_tag="WM481_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081354.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTNFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(660..>1097)
                     /locus_tag="WM481_002141"
     CDS             complement(660..>1097)
                     /locus_tag="WM481_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081355.1"
                     /translation="GNKPAKQATPNGKAGVSTQQSLQNSLVPQKNYTHTTYNGGSAIT
                     DGKYITDPLSMVRHKPDASSLDKIDSKSLFKSDVDAEKATLDAARYADKHNLWDSQNK
                     AKVPTNDVVGYTDGKPTHIINIYRRKPNKDGIRPVHGTPGKER"
BASE COUNT          331 a          193 c          168 g          405 t
ORIGIN      
        1 tgctgaacaa tgttttgtag aacttgctca aaatccatgt ttcgccattt tcctttatat
       61 atacctaatg cagcacgggt tacgatttcg tttaaatcat aaatagccat ttacgtcctc
      121 tgctaatttt cagcgagtag attgggttac caaacccaac aaaaaagcag cctgaaaaat
      181 ttcaggatgc ttgttttatg ttttttatca atgatttagg ttttaatcca gcctgcgctt
      241 gctagttaaa cagtatttac ataaaaatct tgcactgaac aaacaagcgt atcatgttca
      301 tcatgaaatg atgggcataa cgttaatcta caaatgttta catttaaaga tattttttca
      361 gtttcaatat ttttaatttg taaggttaat acatgaggat atttgtgata tagctgattt
      421 tttaattgtt cgttgtctaa tatagcaaaa ggtaattttg cacacaaata accaataact
      481 ttgtttttat gaaaaaaaac tatccaattt tcaattcctt catcccaatc aaaagtaaaa
      541 ttcgttcctt ctatgataaa tttagcacaa gatattaaat ctaacttaac ccaagtggat
      601 ttatacagac tcaatgctga tgaagttata atattatcta aattataaat tttcataaat
      661 caacgttcct tacctggtgt tccatgcaca ggtctaatcc catctttatt cggctttcta
      721 cgataaatgt taattatatg tgtcggtttt ccgtctgtat agccaactac gtcgttcgtt
      781 ggaacttttg ccttattttg actatcccat aaattatgtt tatctgcata tctagcagca
      841 tctaatgtag ctttttccgc atcaacatct gatttaaata aacttttgct atctatcttg
      901 tctagactag atgcatcagg tttgtgtcgt accatactga gagggtcagt aatatatttg
      961 ccatctgtaa tagctgatcc accattatag gtagtatgcg tataattttt ttgtggaacg
     1021 agactatttt gcaaagattg ttgtgtagaa acgccagctt tgccattagg agtagcttgc
     1081 ttcgctggct tattccc
//
LOCUS       DAWTCI010000151         1088 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWTCI010000151 DAWTCI010000000
VERSION     DAWTCI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.151"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..247
                     /locus_tag="WM481_002142"
     CDS             <1..247
                     /locus_tag="WM481_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFA9081356.1"
                     /translation="RDKAVTDDAVKDVTGNDKIETKHLPSTCRCFQIGSVKWFCGQPV
                     TRDNAGTDAVTADTTGKDKEIDTKHLPSTCRDKSSAE"
     gene            276..656
                     /locus_tag="WM481_002143"
                     /pseudo
     CDS             276..656
                     /locus_tag="WM481_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            698..>1088
                     /locus_tag="WM481_002144"
     CDS             698..>1088
                     /locus_tag="WM481_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFA9081357.1"
                     /translation="MAGKQRFCRRGIPPSDIKGKYVKSVTVEKGVVTAKMLSSGVNNE
                     IKNKKLSLWAKREAGSVKWFCGQPVKRNDAANDDVTDDAGTDNGGKGKIDTKHLPSTC
                     RDKSTAVCTKHHAPISNTSKKSAVAGYY"
BASE COUNT          334 a          343 c          248 g          163 t
ORIGIN      
        1 gcgcgacaaa gccgtcaccg acgacgccgt caaagacgtc accggcaacg acaaaatcga
       61 aaccaagcac ctgccgtcaa cctgccgttg ctttcaaatc ggttcggtaa aatggttctg
      121 cggacagccg gttacgcgcg acaacgccgg caccgacgcc gtcaccgccg acaccaccgg
      181 caaagacaaa gaaatcgaca ccaagcacct gccgtcaacc tgccgcgata aatcatctgc
      241 cgaataaggc aaattaggcc ttaaatttca aataaatcag ccatcaccgg gtattaccct
      301 cagccgtcac cgaatattac ccgaataacg gcaaatggcc cgcaaacaac ggcgctgccg
      361 gcgtggcatc cgcttcaaca atcaaaggca aatatgttca gaaagttgaa gtcgcaaaag
      421 gcgtcgtcac cgcccaaatg gcttcaaccg gcgtaaacaa agaaatcaaa ggcaaaaaac
      481 tctccctgtg ggccaggcgt caagacggtt cggtaaaatg gttctgcgga cagccggtta
      541 cgcgcaccga agccaacgcc aaagacgaca ccgtcaccgc cgccaacgac gccggcaacg
      601 gcaccaacat cgaaaccaag cacctgccgt caacctgccg cgataaatca actgccggtt
      661 gcacaaaaac acccgaatat tacctgaatc acggcgaatg gccggaaaac aacgcttctg
      721 ccggcgtggc atccccccct ccgacatcaa aggcaaatat gttaaaagcg ttacggtcga
      781 aaaaggcgtc gttaccgcca aaatgctttc aagcggcgta aacaatgaaa tcaaaaacaa
      841 aaaactctcc ctgtgggcca agcgtgaagc cggttcggta aaatggttct gcggacagcc
      901 ggttaagcgc aacgacgccg ccaacgacga cgttaccgac gacgccggca ccgacaacgg
      961 cggcaaaggc aaaatcgaca ccaagcacct gccgtcaacc tgccgcgata aatcaactgc
     1021 cgtttgcacg aaacaccacg cgccgatttc aaacacttcc aaaaaatcag ccgttgccgg
     1081 gtattacc
//
LOCUS       DAWTCI010000152          994 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWTCI010000152 DAWTCI010000000
VERSION     DAWTCI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.152"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            160..636
                     /locus_tag="WM481_002145"
     CDS             160..636
                     /locus_tag="WM481_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081358.1"
                     /translation="MEANKFEVKSLSDLIKVFAGIAADFEAAMGVKRADISTEFDEPQ
                     HEPQPPVTVAEQKGINDFAIGKEVIIRTYSAGVWFGVLKQKAGNEVILTKARRMYSWW
                     AKESISLSGVARHGIRQDGSQICGELDSVWLEAIEIIPVTGGAAESIRTALEVAQS"
     gene            633..920
                     /locus_tag="WM481_002146"
     CDS             633..920
                     /locus_tag="WM481_002146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081359.1"
                     /translation="MSYLDQPLKHGYGNGNGSGNGSGYGNGYGNGYGNGYGGVGGSGS
                     GSGYGNGKGYSNGSGNGDGDGNGNGSGNGSGNGDGDGNGNGSGNGSGYGNG"
BASE COUNT          295 a          249 c          288 g          162 t
ORIGIN      
        1 tggtgaaatc tgccgcgacg cagcagcgac atggaccact agaaaaagcg aaagcatcaa
       61 tcacaccgcc ttcactttta cagtgaaaaa agacggctta aaagagaagg taatgattgt
      121 tttgtaaaga aaccaacccc accccgaaag gaaacaaaaa tggaagcaaa taaatttgaa
      181 gtgaaaagtt tgtcagacct tataaaagtc tttgcaggca ttgctgccga tttcgaagcg
      241 gcaatgggtg taaaacgcgc cgacatttca accgaatttg acgaaccgca acatgagccg
      301 caaccgccgg taacagttgc cgagcaaaaa ggtatcaacg actttgccat cggcaaggaa
      361 gtcatcatcc gcacttattc ggcaggcgtt tggtttggtg tgttgaaaca aaaagcaggc
      421 aatgaagtga ttctgacaaa agcgcgccga atgtacagct ggtgggcaaa ggaatcaatc
      481 agcctgtcag gtgtcgcacg acacggcatc aggcaagacg gcagccaaat ttgcggcgag
      541 cttgattccg tatggctcga ggcgattgag attatcccag taacaggcgg cgcggctgaa
      601 tcaatccgca ccgcgctgga ggtcgcccag tcatgagtta tctagatcaa ccattgaagc
      661 acggctacgg caacggcaac ggtagcggca acggtagcgg ctatggcaac ggctacggca
      721 acggctacgg caacggctac ggcggcgtcg gcggtagcgg cagcggcagc ggctacggca
      781 acggcaaggg ctacagcaac ggcagcggca acggcgacgg cgacggcaac ggcaacggta
      841 gcggcaacgg tagcggcaac ggcgacggcg acggcaacgg caacggtagc ggcaacggta
      901 gcggctatgg caacggctaa accttaaaac cgcccgaagc tatatctgcc aagccgggga
      961 tagcacaaag cgatgaagta tcgaacttca aagc
//
LOCUS       DAWTCI010000153          978 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWTCI010000153 DAWTCI010000000
VERSION     DAWTCI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.153"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(33..723)
                     /locus_tag="WM481_002147"
                     /pseudo
     CDS             complement(33..723)
                     /locus_tag="WM481_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
BASE COUNT          345 a          168 c          161 g          304 t
ORIGIN      
        1 ctatataaaa taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt
       61 tctaccgtaa aacgtgtttg acgaacggga tttttattaa aaaacatctg atttctaaca
      121 aaatcagtat tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt
      181 ttccatgcgc tcaagaattg tgatttgctc gttgagacgt gccccagcga tggatcagcc
      241 agcaaaacag tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat
      301 tttaaataca caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa
      361 ccttgtgctt caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc
      421 atttgggttt tatccatttt gtctaagatt tccgcttcag aataatgtct gccataaaaa
      481 ttattcagta acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact
      541 atgccggaat ctcgccgtgc tttccaactc cgtacatgga tgttttggta agaagcgggg
      601 gtcaacaaac ataggccaag caaaaactat atttggggcg aaaccaatca aagccgcata
      661 atttatcaat ttataaagat tttttatcat aatatgtata cgcggaataa acgcatgaaa
      721 cataaaaaaa taaaatcata tgcaatttta ttgcatgaat aaatatgaat aaaatagata
      781 atgatgggag taaatacgcc atgtattttg gaagtttaaa tttattaata ataaaataat
      841 ttatcgtagc gcaaataaat cccaaaattg gaacgattag aaaaaaaatt aatacaccca
      901 taatcatgca acctatatta ataaatagct aactaattct attacagaaa ttagttagct
      961 gattaaaagt gattaaca
//
LOCUS       DAWTCI010000154          964 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWTCI010000154 DAWTCI010000000
VERSION     DAWTCI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.154"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            343..594
                     /locus_tag="WM481_002148"
     CDS             343..594
                     /locus_tag="WM481_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081360.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            591..962
                     /locus_tag="WM481_002149"
     CDS             591..962
                     /locus_tag="WM481_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFA9081361.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          339 a          154 c          204 g          267 t
ORIGIN      
        1 gcaggtacgg tttggcgcgg taaaaaagta gaacttaacc cggccaaatg ggattgggtt
       61 aaaaataccg gctatgaaac aactgttacc cgaaagactt tagatgggca aatagctggc
      121 aagaacaagc ctattaaacc ttcttcagga gattatgtct tgccggtagg cagacaaatc
      181 atagatccta ctcgtacatc tttttcacaa gctacagtta gctatcagaa gcgtggtgca
      241 aattacaact atgattctct tgtagctgca atgaacgaaa aaaaatcatg ggttggcgac
      301 agggttgatg ttgttaatat gcctgatgga gcacctacta gtatggataa cacgcgtatt
      361 atggcagcac gtgaagcagg ggtaaaagtg gaagcgaatg ttcataattt taatgaccga
      421 ttatcatcaa aagagagaat caggtttaag catgatggta ttgagcctca aacttgggga
      481 gaagctatcc agctacgaat tagaaagcaa gaaacacaaa aaggagttcc agaagggtgg
      541 agcaaaagat ttcctaacgg aagtatttat gatgtaaagg tacttaggaa atgataaaac
      601 aaaatagttt tgttccgtat cctgaagcaa tgcttcctaa aggatttaaa tatccgcaaa
      661 gttatttaaa attagctcaa tccactcatg ccattaacta cgatgaacaa tattcttttc
      721 cttggtggtt tgaaaatgca gaaagcaata tatcagaagt aattgacatt tattttgaaa
      781 taactggcat tccaaaccta ttaccttttg ctagaaacca agagtgggct gcctgttttg
      841 atatttcaga taaatcaggt aatcctaaaa ttatagtagt taatttagat aatacaaaat
      901 attacgagac ttttgaaaat tttgatactt ggctaaaaga agctgaaaat gatggttggt
      961 agca
//
LOCUS       DAWTCI010000155          959 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWTCI010000155 DAWTCI010000000
VERSION     DAWTCI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.155"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            36..197
                     /locus_tag="WM481_002150"
     CDS             36..197
                     /locus_tag="WM481_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081362.1"
                     /translation="MSFHPETAYTGGGETEPYGPSPEEIKYRQSPETAETRRMTEKQA
                     EGHIKSIIR"
     gene            266..952
                     /locus_tag="WM481_002151"
     CDS             266..952
                     /locus_tag="WM481_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081363.1"
                     /translation="MSLILSVKDESNFKPCPAGSHHATCIRIIDLGTQLVEYQNEQKR
                     QHKILVQWEIDPEGDPEMLMPDGRPYLISRRYTASLHSKSQLATDLKSWRGRDFTPEE
                     RDNFDLRNILGKPCLLSIAHQESSDGKTTYANISAISNKMKSYTPKQPDNAVFAFDLS
                     DPDWANYGLLNEKLREQIAKSPEYAEAVNGRQPPAPPQKQAQAAEGRTEYPQGNAAPA
                     EDIEDDIPFN"
BASE COUNT          293 a          284 c          252 g          130 t
ORIGIN      
        1 gtgcacggcg atgcggaagt gccggagggt aaagaatgag cttccatccc gaaaccgctt
       61 acaccggcgg cggagaaaca gagccgtacg gaccaagccc cgaagaaatc aaataccggc
      121 aaagcccgga aaccgccgaa acacggcgga tgaccgaaaa acaggcagaa ggccacatta
      181 aaagcattat caggtaacgc cgccgcgcaa ggcataaaac agcgcgagac cgatagacga
      241 aacgaccttt aacaggagac cgaaaatgtc attgatttta agtgtgaaag acgaaagcaa
      301 tttcaaacca tgcccggcag gcagccatca cgccacctgc atccgcatca tcgatttggg
      361 tacgcagctc gtcgagtacc aaaacgaaca aaagcggcag cacaaaattt tagtgcagtg
      421 ggaaatcgac ccggaaggcg atccggaaat gctgatgccg gacggcaggc cctacctcat
      481 cagccgccgg tacaccgcca gcctgcacag caaaagccaa ctggcaacag acctcaaaag
      541 ctggcgcgga agggacttta caccggaaga acgcgacaac ttcgatttgc gcaatatttt
      601 gggcaagccc tgcctgttga gtatcgccca ccaagaaagc agcgacggca aaaccaccta
      661 tgccaacatt tccgccatca gcaacaagat gaagagctac accccgaaac agccggacaa
      721 cgccgttttt gccttcgacc tgtcagaccc cgattgggcg aattacggcc tcctgaacga
      781 gaaactgagg gagcagattg ccaaaagccc ggaatatgcc gaagccgtaa acggccgcca
      841 accgccggcg ccgccgcaga aacaggccca agcggcggaa gggcggacgg aatatccgca
      901 aggcaatgcc gcgcctgccg aagacatcga ggacgacatc ccttttaatt aagcccgcc
//
LOCUS       DAWTCI010000156          909 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWTCI010000156 DAWTCI010000000
VERSION     DAWTCI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.156"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..243
                     /locus_tag="WM481_002152"
     CDS             <1..243
                     /locus_tag="WM481_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081364.1"
                     /translation="ATGANQGKISTVSDYFKNIRTRSVHPRLALGYDFGGWRVFSAPA
                     VAAGLHQSLQYYRSRHCRRFRQIHQFRRITPIPQKP"
BASE COUNT          204 a          298 c          216 g          191 t
ORIGIN      
        1 gcaaccggtg caaaccaagg caaaataagc acggtaagcg attatttcaa aaacatccgc
       61 acccgctccg tccacccccg actcgccctc ggctacgatt tcggcggctg gcgcgtcttt
      121 tccgcccccg ccgttgccgc cggcctccac caatcccttc aatattaccg atcccgccat
      181 tgccgccgat tccgtcaaat ccatcaattc cgccgaatca cgcctattcc ccaaaaacct
      241 tgatgcggcg ggctgaagcc cgccctgcaa ccctctctat gcaccccctt gcgagcccga
      301 cactacgcaa catcttgaga acccatcctg tcaagaatac ccgaaccgtc ccgatacacc
      361 gtaatcctaa aacccgccat tcccgcgcag gcgggaatcc agtccgttcg gtttcagtca
      421 tttccaaaaa ttgccgtagc gttaagtttc tagattccca ctttcggggg aatgacgcgg
      481 tgcaggtttc cgtgcggacg gattcgtcat tcccacacag acaggaatcc ggatttgtcc
      541 gcgcggaaac ttatgcgccg tcattcccgc acaggcggga attcaggtct gtcggcatag
      601 gaacttatcg gataaaacgg ttgccccaac cctgcgttct agattcccac tttcgtggga
      661 atgacgattc aggtattcct aatagcaacc cgccacaacc cacctacgcc catcctacgc
      721 atctgccata cagccctgtt ccggagcgcg cggataaggt gcgatgccgc cggcgggcat
      781 catagtcctc tctccctgtg ggagagagct agaaagctgc aaggcttgta ttttagaaga
      841 ctaagggatt tgggaaagat tgtcgaaatt cggggagccc tctcccccac gggggagggg
      901 gcaggttgc
//
LOCUS       DAWTCI010000157          867 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWTCI010000157 DAWTCI010000000
VERSION     DAWTCI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.157"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..277
                     /locus_tag="WM481_002153"
     CDS             <1..277
                     /locus_tag="WM481_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFA9081365.1"
                     /translation="AKARNQSLKDYLARNMQAAGITEIKADDGTFKASFRKSEAVVIL
                     DEAQIPAEFMREAVKTEPDKTAIRKAIESGRQVAGAKIEGRKNLQIR"
     gene            329..544
                     /locus_tag="WM481_002154"
     CDS             329..544
                     /locus_tag="WM481_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081366.1"
                     /translation="MSYLEDVKNALRVIDNLCKEALKEPESLEGYIDEIRDKADEADT
                     SLEFLKDVINYGISDLKNVIEVFEDCV"
     gene            655..>867
                     /locus_tag="WM481_002155"
     CDS             655..>867
                     /locus_tag="WM481_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081367.1"
                     /translation="MQTVATKPTAKQMLAAKRAAKESTRQERAVKRAGTVKNVDRNRL
                     SARSKAQKENIARMLSGAKVSEDEALT"
BASE COUNT          308 a          172 c          226 g          161 t
ORIGIN      
        1 ggcaaaagcg cggaatcaaa gcctgaaaga ctacttggcg cgcaatatgc aggcggcggg
       61 cattaccgaa atcaaagcgg acgacggcac ttttaaagcc tcgttccgca aatccgaagc
      121 cgtcgtgatc ttagacgaag cacaaatccc cgccgaattt atgcgtgagg ccgtcaaaac
      181 cgaaccggac aaaaccgcca tcagaaaagc gattgaaagc ggtcggcaag tagcaggcgc
      241 gaagattgaa gggcggaaga atttgcagat tagataaacc gtagaagatg ttgacgacag
      301 catcccattt tgagttaagg agcaaaaaat gagctatttg gaagatgtaa aaaacgcatt
      361 aagggtaata gataacttat gcaaagaagc attaaaagag cctgaatcgt tagagggtta
      421 tatagacgaa attagggata aagcagacga agcggatacc tctttggaat ttctaaagga
      481 tgtaataaat tatggtatta gcgatttaaa aaatgtaatt gaggtgtttg aagattgcgt
      541 ttgatattaa tggaacaaga catgaactat caatttaaat tcggcgaccc cgtgagagat
      601 acagctttat gattgggcat tgagagaacg gcatcaaaaa atcgagaaaa caaaatgcaa
      661 acagtagcaa caaaaccgac ggcaaaacag atgcttgccg ccaaacgcgc ggcgaaggaa
      721 tcaacgcggc aggaacgcgc cgtcaaacgc gcgggaacag taaaaaacgt tgaccggaac
      781 cggctgtccg cccggtcaaa agcgcaaaaa gaaaacatcg cccggatgtt gtcgggcgca
      841 aaggtatcgg aagacgaagc cctgacg
//
LOCUS       DAWTCI010000158          859 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWTCI010000158 DAWTCI010000000
VERSION     DAWTCI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.158"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            161..721
                     /locus_tag="WM481_002156"
     CDS             161..721
                     /locus_tag="WM481_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HFA9081368.1"
                     /translation="MKKIIFAALAAAAVGTASATYKVDEYHANVRFAIDHFNTSTNVG
                     GFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQPFTGHLKSADIFDAAQYPDIRFVS
                     TKFNFNGKKLVSVDGNLTMRGKTAPVKLKAEKFNCYQSPMAETEVCGGDFSTTIDRTK
                     WGVDYLVNAGMTKNVRIDIQIEAAKQ"
BASE COUNT          236 a          289 c          181 g          153 t
ORIGIN      
        1 aaaacaaagc acggataaca ccgcgctttt tcatccgccc tgttccgccc gcccgttcgt
       61 caaccgcctg caaacccacc gtttaccggc aggaaatgcc ataaatcccc gcctatgtta
      121 ggataaacgc ctttaccggc ccacacaaag gattatcatc atgaaaaaaa tcatcttcgc
      181 cgcgctcgca gcggcagccg tcggcactgc ctccgccacc tacaaagtgg acgaatatca
      241 cgccaacgtc cgtttcgcca tcgaccactt caacaccagc accaacgtcg gcggttttta
      301 cggtctgacc ggttccgtcg agttcgatca agcaaaacgc gacggcaaaa tcgacatcac
      361 cattcccgtc gccaacctgc aaagcggttc gcaacccttc accggccacc tgaaatccgc
      421 cgacatcttc gatgccgctc aatatccgga catccgcttc gtttccacca aattcaactt
      481 caacggcaaa aaacttgttt ccgttgacgg caacctgacc atgcgcggca aaaccgcccc
      541 cgtcaaactc aaagccgaaa aattcaactg ctaccaaagc ccgatggcgg aaaccgaagt
      601 ttgcggcggc gacttcagca ccaccatcga ccgcaccaaa tggggcgtgg actacctcgt
      661 taacgccggt atgaccaaaa acgtccgcat cgacatccaa atcgaagctg caaaacaata
      721 agccttgatg cgaatcagca caaatgccgt cttcatcaaa ccgccctgcc aaccgtaagc
      781 cgatatggcg gagcacaacc cgccgaaaga tattttggcg gcaggcgggg cttgccggaa
      841 agtccggaaa gtaacgacc
//
LOCUS       DAWTCI010000159          831 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWTCI010000159 DAWTCI010000000
VERSION     DAWTCI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.159"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(185..487)
                     /locus_tag="WM481_002157"
     CDS             complement(185..487)
                     /locus_tag="WM481_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081369.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPTAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSINKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(503..820)
                     /locus_tag="WM481_002158"
     CDS             complement(503..820)
                     /locus_tag="WM481_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081370.1"
                     /translation="MAGGNRPLESKNTVTTNNFFENTGYTEKVLRQASNGDYHGFPQS
                     VDAFSENGTVIQIVGGDNIVRHKLYIPGSYKGKDGNFEYIREADGKINHRLFVPNQQL
                     PEK"
BASE COUNT          242 a          165 c          112 g          312 t
ORIGIN      
        1 gcactgtcct gtaataggct cgcggcggca agttgtgctt ccggacttaa atttcctaaa
       61 tcattaatac cttgtagagt ctgctgagcg gctgtatctg atccggtcag cgtaggttag
      121 gttaaaaccc agcattttca acatcttgat gttttactat aaacctattc ttgtgcttcc
      181 tttgttagat aaatttcaaa acaaaagaca cgtatgcatt ctctaatttt atttgagata
      241 aataatgcat tgctttgttg atactgttaa ataaattact tccctcgtaa taccaattat
      301 catatgtttg ataaaaataa ttatctttct tgatataaat atctcccccc aatacaagta
      361 ttctttcatt tataaataaa tttaatatat ctattgcagt ggggacgggt aatgcggctt
      421 ctctaattcc aattcctatc aaagattccc cttgtctcga ataaatttca aaaatttcat
      481 ttaacatatt aatctctatc tactattttt caggaagttg ttggttaggg acaaataatc
      541 tatgattaat ttttccatct gcttctctga tatattcaaa atttccatct tttcctttat
      601 aagatccagg gatataaagt ttatgcctta caatattatc tccgccaact atttgtatta
      661 ctgttccatt ttcagaaaaa gcatctactg attgcggaaa accatgataa tcaccattcg
      721 atgcttgcct taatactttc tctgtataac cggtattttc aaaaaaatta ttcgttgtta
      781 cagtattttt tgattccagc ggtctattcc cccctgccat ctccccatct a
//
LOCUS       DAWTCI010000160          802 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWTCI010000160 DAWTCI010000000
VERSION     DAWTCI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.160"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            141..368
                     /locus_tag="WM481_002159"
     CDS             141..368
                     /locus_tag="WM481_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFA9081371.1"
                     /translation="MNQKQTQCKQIVDYIRNKGCITSLEAYQNLKVTQLAARITDLEG
                     RGFVFAKPKYKVGNCKNPVAHYSIAKSGIEP"
     gene            365..502
                     /locus_tag="WM481_002160"
     CDS             365..502
                     /locus_tag="WM481_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081372.1"
                     /translation="MSREQRIRGARLPVSAYMQAEGAGKAREALDGRVEKVKGVDDEA
                     V"
     gene            486..>802
                     /locus_tag="WM481_002161"
     CDS             486..>802
                     /locus_tag="WM481_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081373.1"
                     /translation="MMKPSESLRAAGRPIAYYPKLAKPLGGVNAAILFGHFFYWNDKT
                     QYESGIYRTAEEIEIETGLSVQEQRTARAKLRERGVLIETEKRIEHRIYYKLNLDAFD
                     DLM"
BASE COUNT          241 a          170 c          238 g          153 t
ORIGIN      
        1 cggttgtaac ggggtgacgg caaatcggaa agacggctga ccgcccggac agacgggcgg
       61 ccgataaaga aaaacccgca cggggcgggt aatccccctg aattgcaggg aagcggttca
      121 ggtaacggcg aaaggcgatt atgaatcaaa aacaaacgca atgcaaacaa attgtcgatt
      181 acatccgtaa caagggatgc atcacatccc ttgaggctta tcagaacctg aaggtgacgc
      241 agcttgcggc acggataacc gacttggaag gcaggggctt cgtgtttgcc aagccgaaat
      301 acaaggtcgg taactgtaaa aatcccgttg cccattactc aatcgcaaag tcaggaattg
      361 aaccatgagc cgggaacaaa gaatacgcgg agcccgcttg ccggtgtcgg cttatatgca
      421 ggcggaaggc gcgggcaagg cacgggaagc gttggacggg cgggtcgaaa aagtgaaagg
      481 ggttgatgat gaagccgtct gaaagtttga gagcggcagg caggccaatc gcgtattacc
      541 cgaaattggc aaagcctttg ggcggtgtaa atgcggcaat attattcggg catttcttct
      601 actggaacga taagacgcag tatgaatcag gcatttaccg aacagcggaa gaaattgaaa
      661 ttgaaaccgg gctgtccgtt caggaacaaa gaacggcacg ggcaaagctg agggaacgcg
      721 gcgtattgat tgagactgaa aaacgaattg aacaccgcat ttactacaaa ctgaatttag
      781 acgcttttga tgatttgatg tt
//
LOCUS       DAWTCI010000161          770 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWTCI010000161 DAWTCI010000000
VERSION     DAWTCI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.161"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            <1..>770
                     /locus_tag="WM481_002162"
     CDS             <1..>770
                     /locus_tag="WM481_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HFA9081374.1"
                     /translation="GTPIAKIEAPGGKGGLSSPTLVDKDLDGTVDIAYAGDRGGNMYR
                     FDLSNSDPNKWSAKAIFEGTKPITSAPAVSRLKDKRVVIFGTGSDLTEDDVLDTKEQY
                     IYGIFDDDKGTGTVKVTVQNGTAGGLLEQNLMQENKTLFLNKRSDGSGSKGWAVKLTG
                     GQRVTVKPTVVLRTAFVTIRKYNDGGCGAETAILGINTADGGALTPRSARPIVPEANT
                     AVAQYSGHKTTSKGKSIPIGCMEKGGKTVCPNGYVYDKP"
BASE COUNT          211 a          200 c          212 g          147 t
ORIGIN      
        1 ggtacgccga ttgcaaaaat cgaagcaccc ggcggcaaag gcgggctttc gtcccccacg
       61 ctggtggata aagatttgga cggcacggtc gatatcgcct atgccggcga ccggggcggc
      121 aatatgtacc gctttgattt gagcaattcc gatcctaata aatggtctgc aaaggctatt
      181 ttcgaaggca caaaaccgat tacctccgcg cccgccgttt cccgactgaa agacaaacgc
      241 gtggttatct tcggtacggg cagcgatttg accgaagatg atgtactcga tacgaaagaa
      301 caatatattt acggtatctt tgacgacgat aaggggacgg ggacggttaa ggtaacggta
      361 caaaacggca cggcaggcgg gctgctcgag caaaacctta tgcaggaaaa taaaacatta
      421 ttcctgaaca agagatccga cggttcgggc agcaagggct gggcggtgaa attgacaggc
      481 ggacaacgcg ttaccgtcaa accgaccgtg gtattgcgta ccgccttcgt aaccatccgc
      541 aaatataacg acggcggctg cggcgcggaa accgccattt tgggcatcaa taccgccgac
      601 ggcggcgcat tgactccgag aagcgcgcgc ccgattgtgc cggaagccaa tacggctgtc
      661 gcacaatatt ccggccataa gacaacctcc aaaggcaaat ccatccctat aggttgtatg
      721 gaaaaaggcg gtaaaaccgt ctgcccgaac ggatatgttt acgacaaacc
//
LOCUS       DAWTCI010000162          716 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWTCI010000162 DAWTCI010000000
VERSION     DAWTCI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.162"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            248..589
                     /locus_tag="WM481_002163"
     CDS             248..589
                     /locus_tag="WM481_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081375.1"
                     /translation="MTKLYAEIAEMEAQDDGTVKVWGYASSEAVDSDGEVIAAAAMKA
                     AIPDYMKFGEVREMHGSNAAGTAIEINVEDDGRTFFGAHIVDPVAVEKVKTGVYKGFS
                     IGGSVTAVCLG"
BASE COUNT          198 a          172 c          189 g          157 t
ORIGIN      
        1 aaaataggga gggatgtcgt gatatttgaa aaatcaataa acggctggaa ataccgcgcc
       61 gtaatggaaa tcagaaccac gcaaaaggag cttgccttga acagccttta tacaacagac
      121 aggattgaat agtcatgaca aaacctgcga cgagtaactg tcaatctttc gattccgtcc
      181 tcatacatcc taagcgtctt tgcaaattct gttcagttat accatttata cagacaggag
      241 caaaccaatg acgaagttat acgcagaaat cgccgaaatg gaggcgcagg acgacggcac
      301 ggtcaaagtt tggggttatg cctcaagcga ggcggtcgat tcggacggcg aagtcatcgc
      361 ggcggcggct atgaaggcgg cgattcccga ttatatgaag ttcggcgagg tgcgcgagat
      421 gcacggcagc aacgcggcgg gaacggcgat tgaaatcaac gtggaagacg acggcagaac
      481 ctttttcggg gcgcatatcg tcgatcccgt tgcagtggag aaagtcaaaa cgggcgttta
      541 caagggcttt tccatcggcg gcagcgttac cgctgtctgc ttaggttgaa tcggcattcg
      601 tacgattacg gcacaccgcc taatctgtac ctgcgtctgt tgcgcccaat accaaaggct
      661 acctgaaaat ctcccaaact tttcaggtag ccttatcacg ttttcaactg ccgcca
//
LOCUS       DAWTCI010000163          692 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWTCI010000163 DAWTCI010000000
VERSION     DAWTCI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.163"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            48..593
                     /locus_tag="WM481_002164"
     CDS             48..593
                     /locus_tag="WM481_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFA9081376.1"
                     /translation="MQNLPQQGSPAKARGKTRGGGSRRDDGGKAARIHPRRARRLPDI
                     GRRPVDATGRGMRVIRLILPYPVSANRYWRIWRNRAVRSAEAAAYKSVVRRIAQEAGA
                     MPSEGAVAVYVRLIPKANKDGGANKTVIDLDNALKVALDALQGVAYHNDRQVRRIAAD
                     YADEPVAGGGLAVEVGELDEK"
BASE COUNT          150 a          188 c          241 g          113 t
ORIGIN      
        1 tttaatgccg tcccgcgcaa ggaagggggg gtctattatt acaaatcgtg caaaacctgc
       61 cgcaacaagg cagtccggca aaagcgcgcg gaaaaacgcg cggcggcggg agccggcgcg
      121 atgacggcgg caaggctgca cggatacatc cgcgccgcgc acgccgcctg cccgatattg
      181 ggcgccggcc tgtggacgca accggcaggg gaatgcgcgt gatacgcctt atcctgcctt
      241 accccgtatc ggcaaaccga tattggcgga tttggcgcaa cagggcggtc aggagcgcgg
      301 aggcggcggc gtataagtcc gtcgtccgcc gtatcgcgca agaagcgggc gcgatgccgt
      361 ccgaaggcgc ggttgccgta tatgtgcggc tgattcccaa agcgaacaaa gacggcggcg
      421 caaacaagac ggtgatcgat ttggacaacg ccctgaaggt tgcgctggac gcgcttcaag
      481 gcgttgccta tcacaacgac aggcaggtgc ggcgcattgc cgccgattac gccgacgagc
      541 cggtcgcagg cggcggtttg gcggtggagg tgggggagtt ggatgaaaag taaaaccgaa
      601 gcggaaaaat cacatctgca aaaagtggcg gatataggtt gtattgtttg ccgcaattgc
      661 gggcggttcg gcgttcctgc cgaggtccgc ca
//
LOCUS       DAWTCI010000164          668 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWTCI010000164 DAWTCI010000000
VERSION     DAWTCI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.164"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(116..667)
                     /locus_tag="WM481_002165"
     CDS             complement(116..667)
                     /locus_tag="WM481_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFA9081377.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSLWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCIIGLV
                     GRFRLQTASGRVAKAGGAVKAGG"
BASE COUNT          184 a          261 c          125 g           98 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaagcaaag aaaccgccat tagcactaca cccccccaag
       61 ctgcggctgc caaacacatc ggctgcttct cgacgcaccc gctgaaaaat gcgccctatc
      121 cgcccgcctt gaccgcaccc cctgcctttg ccaccctgcc cgatgccgtc tgaagcctga
      181 accgccctac cagcccgatg atgcacgaca ccgccggaaa gggcatacac aaagccgtca
      241 gcacataacg cgcattcccc gtattgacca cgtccgcgcc cttcagcagc agcggcagcg
      301 cgttcgccca ctcccacagc gaaggcgcgc tgaacgccaa taccgccacc agccccaaca
      361 tccggaacac cccgatttcc aagacctgcc gccgccgcac cgaacggttg agcaaaaaca
      421 caaccgccaa cccgaaacag accaccgccg ccataccgcc gttggcgaca atctgcaaac
      481 tgttgaacgc aatatcgggc gtaaccgcca acacgccttc cgaatccggc acggcacgct
      541 gcaaatccaa cccctgcaac acattcaaca caaaaccgta gatcaaatca aaaaacacca
      601 cgcctatcgg caaagcactc aaaacccgcc acatcttcct atctgaccaa ccgttcaaaa
      661 ccttcatt
//
LOCUS       DAWTCI010000165          655 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWTCI010000165 DAWTCI010000000
VERSION     DAWTCI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.165"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(160..444)
                     /locus_tag="WM481_002166"
     CDS             complement(160..444)
                     /locus_tag="WM481_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFA9081378.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMPVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWRDLWFDII"
BASE COUNT          182 a          169 c          119 g          185 t
ORIGIN      
        1 aggtctgtcg gcacagaaac ttatcgggta aaacggtttc tttagatttt acgttctaga
       61 ttcccgcctg cgcgggaatg acggtttaga agttgcccga aacctcaaaa aaaaccgaaa
      121 ccgaacaagc cggattcccg cctgagcggg aatgacgggc taaataatat caaaccataa
      181 atcccgccaa gaaacattat tttcttcaat cagttgcaat ttccaagccc tgttccattt
      241 cttcaactgt ttttcccgag taattgcgct ctccatcgta ggatgcagtt cataccaaac
      301 cggcatagta acgttgtacc gtgatgtaaa tccttcaatc aaatgctccc tatgttggta
      361 aatacgttgc accaaatcag atgtaacgcc aatgtataac gtgccattac gttggcttgc
      421 taaaatataa accgcaggct gcatataata cccttttgaa ttatttaaat ttatattccc
      481 gcgaacacca tccggtgatt actttaaccc ttcgttatcc ccatagcttt ccatcattcc
      541 cgcaactctt cgtcattccc gcgaaagcgg gaatccagaa tctctaaagc ttcagctaac
      601 ctttgaatat tgctgttgtc ccacgttcta gattcccgcc tgcgcgggaa tgacg
//
LOCUS       DAWTCI010000166          655 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWTCI010000166 DAWTCI010000000
VERSION     DAWTCI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.166"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            complement(1..387)
                     /locus_tag="WM481_002167"
                     /pseudo
     CDS             complement(1..387)
                     /locus_tag="WM481_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          180 a          121 c          119 g          235 t
ORIGIN      
        1 atatttcgag ggtaacatat ctgctaatct ggtacagacc cgaatagtgt catgttcttc
       61 actaagagat aatttatctt cattatatgg attaccgagt tttaaaaaat ttgctgcttt
      121 ttctatagca acttgccaag ttttatattt atcgtaattt ttatcaaaac gtactccccc
      181 ttgtttattg gataaaaaag taattatctg attcatatta aaaatatttc cattatggaa
      241 aactcgtttt aaagttaaaa atttttcagc aggacataaa attatatttg tttcagcata
      301 gattattggt tcaccagaaa attcttgtga tgaatgatat atagggctaa taatttttcc
      361 gcctaaataa actccaccag ccatatagag tggattaaat ttaaaccggt acggcgttgc
      421 ctcgccttgc cgtactattt gtactgtctg cggctcgccg ccttgtcctg atttttgtta
      481 atccgctata ttatttaagc gaacttcttg gtttttgttt ggttcgttac ggtcgctccg
      541 gataatcagc cctttcgggc gcgaatgctg tgaagggtcg gcaggtttga ccgtaatatc
      601 cgcattgccg gttacggtat tgtccgcatt attaaactca aaaccgatga gttcg
//
LOCUS       DAWTCI010000167          610 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWTCI010000167 DAWTCI010000000
VERSION     DAWTCI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.167"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            58..273
                     /locus_tag="WM481_002168"
                     /pseudo
     CDS             58..273
                     /locus_tag="WM481_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038789.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a          122 c          115 g          182 t
ORIGIN      
        1 tccctaagaa ggggcgatta acaaaaatta atgccctttc ttagggacgc aatatataag
       61 gtttataccg tcgttgtccc taatgcgcaa tcagcgacat tgttgccaat tatccgaaag
      121 aaagttaagc ctgatggcat tgtatacacg gatacctttc gtagttatga tgtgcttgat
      181 gttagtgaat ttagccattt acgtaagttt aacggcattc ccaaagagca tttggggcta
      241 tatttaaaga aatgccaatg gcatttgaaa tagaatctca aatttagatt ttaagacaat
      301 tagttaatgg gaatttggtc tggttatcta gtacaacccc aaaagttttt atataaaaac
      361 agtttggtaa gttctcttcg tgaagtgctt atgttttgcc acaaaagttc cataatcatg
      421 gcggcatcac ccaagctcca tccttccaag aaaatggcat ctttgttgtg gttgaaaatc
      481 gactccatac gattttggac aagcataggg atagatgttc tgaaaaacgg cagcgcgggc
      541 attctccaac caccttccca aatcttataa tacaaacccg aagacctttg caaaattccc
      601 ctaaaatccc
//
LOCUS       DAWTCI010000168          562 bp    DNA     linear   BCT 29-MAR-2024
DEFINITION  TPA_asm: Neisseria gonorrhoeae isolate 54771
            SAMN23566291-rid13878813.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWTCI010000168 DAWTCI010000000
VERSION     DAWTCI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN23566291
            Sequence Read Archive: SRR17121102
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria gonorrhoeae
  ORGANISM  Neisseria gonorrhoeae
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001241786.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001241786.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/07/2022 00:04:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,169
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 99 of 166
            Pseudo Genes (incomplete)         :: 69 of 166
            Pseudo Genes (internal stop)      :: 31 of 166
            Pseudo Genes (multiple problems)  :: 33 of 166
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Neisseria gonorrhoeae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN23566291-rid13878813.denovo.169"
                     /isolate="54771"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:485"
                     /country="Canada"
                     /collection_date="2018"
                     /collected_by="National Microbiology Laboratory, Winnipeg"
                     /note="genotype: ST-16288"
     gene            133..>562
                     /gene="mafB"
                     /locus_tag="WM481_002169"
     CDS             133..>562
                     /gene="mafB"
                     /locus_tag="WM481_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFA9081379.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNGRGSVKNRVCAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGSVDGGFTVYQLHRTGSEIHPEDG"
BASE COUNT          159 a          134 c          138 g          131 t
ORIGIN      
        1 aactccgccc catccgtaga ggctgataac agtcatgagg ggtatggata cagcgatgaa
       61 gcagtgcgac aacatagaca agggcaacct tgattcacac tgccataacc gcttgctgcc
      121 aaggaaaaca aaatgaattt gcctattcaa aaattcatga tgctgtttgc agcggcaata
      181 tcgttgctgc aaatccccat tagtcatgcg aacggtttgg atgcccgttt gcgcgatgat
      241 atgcaggcaa aacactacga accgggtggc aaataccatc tgttcggtaa tggtcgcggc
      301 agtgttaaaa atcgggtttg cgccgtccaa acatttgatg caactgcggt cggccccata
      361 ctgcctatta cacacgaacg gacagggttt gaaggcatta tcggttatga aacccatttt
      421 tcaggacacg gacacgaagt acacagtccg ttcgataatc atgattcaaa aagcacttct
      481 gatttcagcg gcagcgtaga cggcggtttt accgtttacc aacttcatcg gacagggtcg
      541 gaaatccatc cggaggatgg at
//
